LOCUS       DAVKWZ010000001        79028 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000001 DAVKWZ010000000
VERSION     DAVKWZ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 79028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79028
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.001"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(271..1878)
                     /locus_tag="VW854_000001"
     CDS             complement(271..1878)
                     /locus_tag="VW854_000001"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEV0261318.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(2268..2855)
                     /locus_tag="VW854_000002"
     CDS             complement(2268..2855)
                     /locus_tag="VW854_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEV0261319.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            complement(2983..3432)
                     /locus_tag="VW854_000003"
     CDS             complement(2983..3432)
                     /locus_tag="VW854_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0261320.1"
                     /translation="MCFVPYYKVNHCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKEIYTYNHASRRCFKSLGFVENGATEKGRSFILELV"
     gene            complement(3437..3826)
                     /locus_tag="VW854_000004"
     CDS             complement(3437..3826)
                     /locus_tag="VW854_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEV0261321.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGRHRAYQTLLADRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            complement(3854..4480)
                     /locus_tag="VW854_000005"
     CDS             complement(3854..4480)
                     /locus_tag="VW854_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV0261322.1"
                     /translation="MKGSFALLLFVILGYMVKFYPETLVNFDQSIQTAIRGDLPDYLT
                     ILFRALTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIVTFKNIYQR
                     PRPDILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDPVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            complement(4494..5057)
                     /locus_tag="VW854_000006"
     CDS             complement(4494..5057)
                     /locus_tag="VW854_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV0261323.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTIGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     regulatory      complement(5222..5456)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(5510..6013)
                     /locus_tag="VW854_000007"
     CDS             complement(5510..6013)
                     /locus_tag="VW854_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEV0261324.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKIYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     gene            complement(6206..7036)
                     /locus_tag="VW854_000008"
     CDS             complement(6206..7036)
                     /locus_tag="VW854_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV0261325.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDISFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            complement(7021..8703)
                     /locus_tag="VW854_000009"
     CDS             complement(7021..8703)
                     /locus_tag="VW854_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0261326.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQVAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LISDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLANLDNMSIS
                     KGQVQLQNELAKETPELQSLFKLEDVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            complement(8805..9107)
                     /locus_tag="VW854_000010"
     CDS             complement(8805..9107)
                     /locus_tag="VW854_000010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261327.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEIVDRTDEVSSKHGFEVVDETDEVS
                     SKHGFEVVDETDEVSNHTYGKAKLTWFEEIFEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            complement(9114..9662)
                     /locus_tag="VW854_000011"
     CDS             complement(9114..9662)
                     /locus_tag="VW854_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEV0261328.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLAGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            complement(9677..10525)
                     /locus_tag="VW854_000012"
     CDS             complement(9677..10525)
                     /locus_tag="VW854_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEV0261329.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            10713..12062
                     /gene="trkA"
                     /locus_tag="VW854_000013"
     CDS             10713..12062
                     /gene="trkA"
                     /locus_tag="VW854_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEV0261330.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEHDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            12066..13505
                     /locus_tag="VW854_000014"
     CDS             12066..13505
                     /locus_tag="VW854_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV0261331.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSVIDAF
                     FEISSGFTTTGASILNDISVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNQTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(13631..15310)
                     /locus_tag="VW854_000015"
     CDS             complement(13631..15310)
                     /locus_tag="VW854_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV0261332.1"
                     /translation="MDKLVAAIEKQQGKFEKISTNNYMMAIKDGFIATMPLIMFSSFL
                     MIIIMIPKNFGVELPSPAIAWMRKVYMLTMGVLGIIVSGTVAKSLAGNVNRKMPPGKV
                     INDTSAMLAAICGYLVLTVTLVVDEKTGSTSLSTNYLGSQGLITSFVSAFITVNVYRF
                     CIKRDITIHLPKEVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLL
                     TPIFKGAESYPAMMLIWFMCALLWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQF
                     PYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKATITPVLFAVNEPLL
                     FGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWAFPGPLGLLIGTNF
                     QLISFVFLSLMLVVDILIYLPFCRAYDRQLLVKEDIASSNDIILEEDTSEIMPGEIDV
                     IKSKELKVLVLCAGSGTSAQLANAINEGAQLAEVRVIANSGAYGAHYDIMGVYDLIIL
                     APQVRSYYREMKVDAERLGIQIVATRGMEYIHLTKSPSKALQFVLEHYQAV"
     gene            complement(15380..16792)
                     /gene="lacG"
                     /locus_tag="VW854_000016"
     CDS             complement(15380..16792)
                     /gene="lacG"
                     /locus_tag="VW854_000016"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEV0261333.1"
                     /translation="MENLQVKALPKEFLLGAATAAYQVEGATRVDGKGTNMWDVYLQE
                     NSPFLPDPASDFYYRYEEDIALAAEHGLQALRLSISWVRIFPDIDGEPNILAVRYYHR
                     VFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGKVGC
                     IHALKPGYPIDGQKENILAAKRYDVYNNKFLLDGTFLGYYSEDTLFHLNQILEANNSS
                     FIIEDGDLEIMKRAAALNTMFGMNYYRSEFIREYKGENRQEFNSTGIKGQSSFKLNAL
                     GEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGEN
                     DIIDDSKRIRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDY
                     ETQKRYIKKSALWVKGLKRN"
     gene            complement(16802..17146)
                     /locus_tag="VW854_000017"
     CDS             complement(16802..17146)
                     /locus_tag="VW854_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV0261334.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEASGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            complement(17314..19200)
                     /locus_tag="VW854_000018"
     CDS             complement(17314..19200)
                     /locus_tag="VW854_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HEV0261335.1"
                     /translation="MVIRVFDQQKNTYSSFALEELSYYMNRVFKTNIELVEEKEADIF
                     VGLVNKEDRKDHVLISLDKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGRRHDFVP
                     ELRFEDFLDKQLSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEQFSNELVQELSDRLDKELQKRGLIHHRVGHGWTGEVLGY
                     SSKFGWESGLSISEEKKPYVAEINGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHVWLSDARNNICECENCRQELVSDQYIRILNQLDRALTSEGLDTKICFL
                     LYHELLWAPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPTPKPYLRNKIILP
                     NSLEENLSYLFEWQKTFKGDSFVYDYPLGRAHYGDLGYMKISQTIYRDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGEMLWKKTRSYEELIEEYFSALYGSNWQSVVEYLEKL
                     SSYSSCDYFNAIGSRQNDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWRNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GFHL"
     gene            complement(19202..20524)
                     /locus_tag="VW854_000019"
     CDS             complement(19202..20524)
                     /locus_tag="VW854_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV0261336.1"
                     /translation="MNNILAFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSL
                     FLILISWPQEDFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPS
                     AGIIALSSFVLMAPRFSSMVYDKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAE
                     IYRMFIQRGITIKMPSGVPDVVSKSFSALLPGFTTFVLWALVLKGLEAAGVAGGLNGL
                     LGAIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDPVWLQFTTENQEA
                     VAAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFN
                     INTAILFTFPTVLNPIMLIPFIATPTINALITYVSMAVGLVPYTTGVILPWTMPPIIG
                     GFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVGGK"
     gene            20692..21915
                     /locus_tag="VW854_000020"
     CDS             20692..21915
                     /locus_tag="VW854_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEV0261337.1"
                     /translation="MITKQSAFLQNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPYVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTAIEYVPLETKLLGFGLSIPGHYNKDSGSI
                     ITNNPIWESFNLLNVIKRFNFPFIVKNNIDCMAIGQYLFNPHNTPDNFIFLHAGLGIY
                     TSFFTKEKIGASKNPYIGEIGHTIVELNGQYCECGKKGCLQTYISDAWLIKHAQLLFK
                     NSQLTVLKSLVKTEKDIHLDTLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPAD
                     KIYINSQLLNYQPFTHEVRDKIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVA
                     FFIEHSNVLQDIISP"
     gene            22565..22735
                     /locus_tag="VW854_000021"
     CDS             22565..22735
                     /locus_tag="VW854_000021"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0261338.1"
                     /translation="MTKQVPKFTKDTAQLYTCKWLLYNKVTKMYDHTVVNHSVREYIT
                     DSISTNTIEETG"
     gene            23348..23632
                     /locus_tag="VW854_000022"
     CDS             23348..23632
                     /locus_tag="VW854_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261339.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            23625..23863
                     /locus_tag="VW854_000023"
                     /pseudo
     CDS             23625..23863
                     /locus_tag="VW854_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397100.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23959..24177
                     /locus_tag="VW854_000024"
     CDS             23959..24177
                     /locus_tag="VW854_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261340.1"
                     /translation="MMSIREQDLQDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
     gene            complement(24549..26873)
                     /gene="pflB"
                     /locus_tag="VW854_000025"
     CDS             complement(24549..26873)
                     /gene="pflB"
                     /locus_tag="VW854_000025"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEV0261341.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            27268..28329
                     /gene="dinB"
                     /locus_tag="VW854_000026"
     CDS             27268..28329
                     /gene="dinB"
                     /locus_tag="VW854_000026"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEV0261342.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIRDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKSVGLRIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGISYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILRAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSIAQKTQDASQISQIALQLYE
                     ELSEKERGVRLLGITMTGF"
     gene            complement(28476..29321)
                     /locus_tag="VW854_000027"
     CDS             complement(28476..29321)
                     /locus_tag="VW854_000027"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEV0261343.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            29577..30445
                     /locus_tag="VW854_000028"
                     /pseudo
     CDS             29577..30445
                     /locus_tag="VW854_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841243.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(30746..32635)
                     /locus_tag="VW854_000029"
                     /pseudo
     CDS             complement(30746..32635)
                     /locus_tag="VW854_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            32773..34338
                     /locus_tag="VW854_000030"
     CDS             32773..34338
                     /locus_tag="VW854_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV0261344.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYN
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKVIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            34338..35078
                     /locus_tag="VW854_000031"
     CDS             34338..35078
                     /locus_tag="VW854_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV0261345.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(35187..35402)
                     /locus_tag="VW854_000032"
     CDS             complement(35187..35402)
                     /locus_tag="VW854_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261346.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
     gene            complement(36137..37387)
                     /gene="ilvA"
                     /locus_tag="VW854_000033"
     CDS             complement(36137..37387)
                     /gene="ilvA"
                     /locus_tag="VW854_000033"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEV0261347.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIKTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(37437..37793)
                     /locus_tag="VW854_000034"
     CDS             complement(37437..37793)
                     /locus_tag="VW854_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV0261348.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEVFGDE"
     gene            complement(38031..38267)
                     /locus_tag="VW854_000035"
     CDS             complement(38031..38267)
                     /locus_tag="VW854_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261349.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMNFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKH"
     gene            complement(38374..39396)
                     /gene="ilvC"
                     /locus_tag="VW854_000036"
     CDS             complement(38374..39396)
                     /gene="ilvC"
                     /locus_tag="VW854_000036"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV0261350.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDAYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(39462..39938)
                     /gene="ilvN"
                     /locus_tag="VW854_000037"
     CDS             complement(39462..39938)
                     /gene="ilvN"
                     /locus_tag="VW854_000037"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEV0261351.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(39931..41631)
                     /locus_tag="VW854_000038"
     CDS             complement(39931..41631)
                     /locus_tag="VW854_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEV0261352.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            complement(41831..43498)
                     /locus_tag="VW854_000039"
     CDS             complement(41831..43498)
                     /locus_tag="VW854_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEV0261353.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFCGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDAVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(43501..43866)
                     /locus_tag="VW854_000040"
     CDS             complement(43501..43866)
                     /locus_tag="VW854_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV0261354.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(44023..44211)
                     /locus_tag="VW854_000041"
     CDS             complement(44023..44211)
                     /locus_tag="VW854_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEV0261355.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            complement(44327..44812)
                     /locus_tag="VW854_000042"
     CDS             complement(44327..44812)
                     /locus_tag="VW854_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEV0261356.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIVNVQIDSSILKGYRLPFEGLSYAAFFRYFIPKYVSESRVL
                     YLDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLINNGMWRAENVTQDL
                     I"
     gene            complement(45081..46625)
                     /locus_tag="VW854_000043"
     CDS             complement(45081..46625)
                     /locus_tag="VW854_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEV0261357.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLETFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMSPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            46838..47140
                     /gene="gatC"
                     /locus_tag="VW854_000044"
     CDS             46838..47140
                     /gene="gatC"
                     /locus_tag="VW854_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEV0261358.1"
                     /translation="MKITQEEVTHVANLSKLRFSEKETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEKDNYYIKVPAILDDGGDA"
     gene            47140..48606
                     /gene="gatA"
                     /locus_tag="VW854_000045"
     CDS             47140..48606
                     /gene="gatA"
                     /locus_tag="VW854_000045"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEV0261359.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQTTLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            48606..50048
                     /gene="gatB"
                     /locus_tag="VW854_000046"
     CDS             48606..50048
                     /gene="gatB"
                     /locus_tag="VW854_000046"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEV0261360.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVKLEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LDGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            50177..50737
                     /gene="efp"
                     /locus_tag="VW854_000047"
     CDS             50177..50737
                     /gene="efp"
                     /locus_tag="VW854_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEV0261361.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            50759..51148
                     /locus_tag="VW854_000048"
     CDS             50759..51148
                     /locus_tag="VW854_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV0261362.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            51141..51563
                     /gene="nusB"
                     /locus_tag="VW854_000049"
     CDS             51141..51563
                     /gene="nusB"
                     /locus_tag="VW854_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEV0261363.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(51613..51849)
                     /locus_tag="VW854_000050"
     CDS             complement(51613..51849)
                     /locus_tag="VW854_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0261364.1"
                     /translation="MDVILSAIIFGISHLILSHRDPISLLYYSLIGFFFALVYRSTDN
                     LRLTILCHSFFNFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(51862..52335)
                     /locus_tag="VW854_000051"
     CDS             complement(51862..52335)
                     /locus_tag="VW854_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261365.1"
                     /translation="MRNTKKLRQFGIFLLIILLSTYLPQTIGLYVTIILGLGADVYSL
                     ILTMGLVGSFLLLIWRLKKKKMLFIFEKKSWNWSFVFYLFATYVVYQILGNFWARYAH
                     LINHRNIHDEYFTVLFSNGQPTFLSTILSFVLPVIIGPVFEETLDRGYFMNTFFP"
     gene            complement(52353..52538)
                     /gene="briC"
                     /locus_tag="VW854_000052"
     CDS             complement(52353..52538)
                     /gene="briC"
                     /locus_tag="VW854_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HEV0261366.1"
                     /translation="MTGTNTFTVLSTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRAG"
     gene            complement(52697..53464)
                     /locus_tag="VW854_000053"
     CDS             complement(52697..53464)
                     /locus_tag="VW854_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEV0261367.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEIGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(53461..54327)
                     /locus_tag="VW854_000054"
     CDS             complement(53461..54327)
                     /locus_tag="VW854_000054"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEV0261368.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(54364..55731)
                     /gene="accC"
                     /locus_tag="VW854_000055"
     CDS             complement(54364..55731)
                     /gene="accC"
                     /locus_tag="VW854_000055"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEV0261369.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(55743..56165)
                     /gene="fabZ"
                     /locus_tag="VW854_000056"
     CDS             complement(55743..56165)
                     /gene="fabZ"
                     /locus_tag="VW854_000056"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEV0261370.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGTLTFAIGN"
     gene            complement(56162..56647)
                     /locus_tag="VW854_000057"
     CDS             complement(56162..56647)
                     /locus_tag="VW854_000057"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEV0261371.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(56650..57885)
                     /gene="fabF"
                     /locus_tag="VW854_000058"
     CDS             complement(56650..57885)
                     /gene="fabF"
                     /locus_tag="VW854_000058"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEV0261372.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(57907..58638)
                     /gene="fabG"
                     /locus_tag="VW854_000059"
     CDS             complement(57907..58638)
                     /gene="fabG"
                     /locus_tag="VW854_000059"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEV0261373.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVVAIDGGLSM"
     gene            complement(58672..59592)
                     /gene="fabD"
                     /locus_tag="VW854_000060"
     CDS             complement(58672..59592)
                     /gene="fabD"
                     /locus_tag="VW854_000060"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEV0261374.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            complement(59585..60559)
                     /gene="fabK"
                     /locus_tag="VW854_000061"
     CDS             complement(59585..60559)
                     /gene="fabK"
                     /locus_tag="VW854_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEV0261375.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVD
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWAGVVRND"
     gene            complement(60678..60902)
                     /locus_tag="VW854_000062"
     CDS             complement(60678..60902)
                     /locus_tag="VW854_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV0261376.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(60962..61936)
                     /locus_tag="VW854_000063"
     CDS             complement(60962..61936)
                     /locus_tag="VW854_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEV0261377.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGKELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(61936..62370)
                     /locus_tag="VW854_000064"
     CDS             complement(61936..62370)
                     /locus_tag="VW854_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV0261378.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(62464..63249)
                     /locus_tag="VW854_000065"
     CDS             complement(62464..63249)
                     /locus_tag="VW854_000065"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEV0261379.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            63574..64938
                     /locus_tag="VW854_000066"
     CDS             63574..64938
                     /locus_tag="VW854_000066"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEV0261380.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEDNEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREIGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            64941..65312
                     /locus_tag="VW854_000067"
     CDS             64941..65312
                     /locus_tag="VW854_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEV0261381.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            65526..66800
                     /gene="serS"
                     /locus_tag="VW854_000068"
     CDS             65526..66800
                     /gene="serS"
                     /locus_tag="VW854_000068"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEV0261382.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(66825..67724)
                     /locus_tag="VW854_000069"
     CDS             complement(66825..67724)
                     /locus_tag="VW854_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEV0261383.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEMAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(67834..68382)
                     /locus_tag="VW854_000070"
     CDS             complement(67834..68382)
                     /locus_tag="VW854_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV0261384.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(68600..69922)
                     /locus_tag="VW854_000071"
     CDS             complement(68600..69922)
                     /locus_tag="VW854_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEV0261385.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPAITSLVLSVFVAIAVFGGLKSISKVSTTVVPFMALIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGVRFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(70013..70101)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(70265..72601)
                     /locus_tag="VW854_000072"
     CDS             complement(70265..72601)
                     /locus_tag="VW854_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEV0261386.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVYFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(72705..73253)
                     /locus_tag="VW854_000073"
     CDS             complement(72705..73253)
                     /locus_tag="VW854_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEV0261387.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(73250..73552)
                     /gene="zapA"
                     /locus_tag="VW854_000074"
     CDS             complement(73250..73552)
                     /gene="zapA"
                     /locus_tag="VW854_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEV0261388.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            73639..74520
                     /locus_tag="VW854_000075"
     CDS             73639..74520
                     /locus_tag="VW854_000075"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEV0261389.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TPDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQVLLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLAVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLNFC
                     AKLHFKNTEKAKKRLER"
     gene            74524..75138
                     /gene="lepB"
                     /locus_tag="VW854_000076"
     CDS             74524..75138
                     /gene="lepB"
                     /locus_tag="VW854_000076"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEV0261390.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            75266..77632
                     /locus_tag="VW854_000077"
     CDS             75266..77632
                     /locus_tag="VW854_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEV0261391.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLSKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLKTIQKHITTIEKELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDLRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSEESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(77680..78963)
                     /locus_tag="VW854_000078"
     CDS             complement(77680..78963)
                     /locus_tag="VW854_000078"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEV0261392.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVITAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     ATAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
BASE COUNT        23824 a        17616 c        14482 g        23106 t
ORIGIN      
        1 ctgatagaga tgttcacatc ttatttcaat tcactataca atccagttcc taaaactttg
       61 ctagaaattg atttggcttt ccgaattaat ttgttcaaat tttatttcat ttcaatatac
      121 tgataaatcc attcactagc acagggcaag agggagtcgc tattgatttc aacggtagca
      181 ggtacataca gaggacccct acctcagata tactgcaata tcatcgtatg caaaaaacct
      241 ctgagatttt tctcaaaggt tctgattttg ctaattgctg ttctcaaccg ctgcagtaac
      301 aaaggcagtg tagagttctt ctggtcggtt tggacggctt gacagttcag ggtgatactg
      361 acaagctaca aagaatttat tttcaggaat ttccacgatt tctaccaaac gattgtctgg
      421 agaaactcct gaaaagacaa aacctgctgc ctcaaactgc tcacggaagg cattattaaa
      481 ctcataacgg tgacggtgac ggcgttgcac cacttcttga ttgtgataag cagcagcagc
      541 cttagagcca cgtttcaact tagacggata aagtcccaaa cgaagggttc cacccatatc
      601 ctcaatatca atctgatcac gcatgatatc aatgataggg tattttgttt ctggtgcaag
      661 ctctgcagaa ttggcacctt caagacctaa aacgtgacga gcaaactcga tacatgtcaa
      721 ctgcattccc aagcagactc ccaacattgg aacatcattt tcacgcgcat agcggatggc
      781 ttggattttc ccttctgtac cacgttgacc aaaaccacct ggtacgatga tcccgtccgc
      841 atcagacaag agttctgcta cattctctgc tgtcacatca ttggcattga cccaattgat
      901 tttaacttct acatcattga catagccaga gtgtttcaag gcttcgacca ctgagatata
      961 ggcatcttgc aactccacat acttaccaac aagggaaatc ttaacttgtt tcttgaggtt
     1021 catgaccttg tccaccatgg ctgaccattc tgtcatatcc gctgctggtg cgtctaattt
     1081 caaatgatca caaacaattt ggtccatccc ttgtgcctgc aagttcagtg gaatttggta
     1141 aaggtgttca acatccaacg attcgataac ggcttctggt gccacatcac agaactgggc
     1201 cagtttattt ttaattcctt gaccagctgg ctcttctgta cgaataacca acatatttgg
     1261 ttggattccc aagccacgca attctttgac agagtgttgg gttggtttgg ttttcatttc
     1321 accagcagcc ttgaggtaag gaagcaaggt tgtatggata tacatgacat tatccgcacc
     1381 cacatctgcc ttcatctgac gaagagcctc taggaatggc aaggactcga tatctcctac
     1441 tgttccacca acctctgtga taatgacatc agagtcggtc gttagagcgg cacgcttgat
     1501 tttttctttc aaagcatctg tgatatgagg aatgacttga acagttgccc caaggtattc
     1561 tccacggcgt tctttacgaa gaacttcact gtaaattttc ccagttgtca cgttggaata
     1621 tttgttgaga ttgatatcga tgaaacgttc atagtgaccc aagtccaaat ctgtctcagc
     1681 tccgtcatct gtcacaaaaa cttccccgtg ctggtaagga ctcatggttc ccggatcaat
     1741 attgatataa gggtcaaact tttgaatggt tactttgaga ccacgatttt tcaagagacg
     1801 gcctagactc gctgccacaa tccctttccc aatagacgat accacaccac cagttacaaa
     1861 aatatatttc gtagacatag attcctcttt ctaaaatgct caaggtcttg ttaactacga
     1921 ggggatatag aaaacaagac cctactaata agaataatct gggacgactg caccagattc
     1981 acagctaata ctcttcgaaa atctcttcaa accgcgtcaa cgtcgccttg ccgtagatat
     2041 gtgttcctga ctttgtcagt cttatctaca acctcaaaat agtgttttga gcagcctacg
     2101 gctagtttcc tagcttgctc tttgattttc attgagtata aaatacaaga agataacttc
     2161 ttgaaaaaac aaaaatagct ccctaagaac tagggagccc cgacctctaa aagaggtgcc
     2221 caaacaatat gatacctaaa aataggataa ttgtcaatag ctaactctta ttcctcgctg
     2281 gtttcagcgt cgtcatctga aaactcgtcg tcgtcttcgt tgagatccac atcttctccc
     2341 aagtcatctg gagcgatttc gttgatttct gcatcataag cttccacttc atttttttca
     2401 tcatctggat tttcatcatc gtatgaaaga gctggatctg cttcgtatgc atcttcgtct
     2461 tctggatcat ctgcattgta gtcaatggca tctgaatcac catccataaa ggcattgaca
     2521 cgttttttct tagcttttgg tgctacttca tcgtcgtcat tttcttcaag agcgatgatt
     2581 tcttcgtcga tttcgtccac accataccat gaacgaagac cccatttgtt gtccccaagt
     2641 gagatgaagc taccgtcaaa gttcaactct gtgtagaaca aaggcaaagc ttcgcggata
     2701 tcgctgtttg atgttccaag gtagttttga atttcgttta caagatcgct aaaatgcatc
     2761 tcatgatcgc gaccacgaag ttccaagata gcacgcgcta cctcaatcat agatagttca
     2821 cttttttctt gcccagcaaa tacttctaat tccaaagcgt ttctcctcat ttatactact
     2881 atcgccagag cgaacagact ctgacctcat tttatcattt actctttatt ttacgataat
     2941 tttgcagaat agtcaaaggt taagggggag aaaatggcag gattagacta attccaatat
     3001 aaaactcctt cctttttctg ttgctccatt ttccacaaat ccaagcgact tgaaacacct
     3061 cctagaagca tgattgtagg tgtagatttc cttgactctc aattctttcc atccttttac
     3121 tcgagccaat tcaatcaaag cacttagaat cttttttcca agtcctcgat gttggtaagc
     3181 ggaattccca atcacaatgg ggagattatc ctgagatagt gtaatatccc caattggaaa
     3241 ccattctccc ttctccttga cttcaatcca aaaaagctca ccatgccgat ccaaatagga
     3301 atacatggct tccaaggtcg cttgactgta aggaagcttc accccatcta cgaggtaaac
     3361 caagttcaca tcctgatacc aagcaaaagc ttcctcacaa tgattgacct tataataagg
     3421 aacaaaacac aatgcactac cttttcaaca aaagagttgc tgcttgatta aaaccatcac
     3481 accagttata ccattttgct tcatacccat cttgagctag gatacgatct tctaaatcaa
     3541 aaacagagta aatctttctt tcctcgcaag cttgcgcata gagatgatat agttcatcac
     3601 caccatctct atcccactca gcagaaatcg tatcccgatc tgccaataaa gtctgataag
     3661 ccctgtgacg cccatctgta atcagcaaac aatctccaaa ggcaagaata ctgattggat
     3721 cgacttggat tgtttctgcc gactggtaaa gcatctgaat atcttgcaac ttcttttctg
     3781 ataaatatag ttgagtcgga tgaagatctg ctatgttgac tttcatttct ttctcctcaa
     3841 gggaattcga tactcacttc tgtttgcctt taaatcgcca ttggaagcgg agcttgtcat
     3901 agaagggaaa ctcgataaac aggactccca agcccacaca gagactggca aggacgtctg
     3961 atgggtaatg aactcccaga tagactcttg ataccagcac actgactagg tagaggccaa
     4021 ggacgatttg tacgattttt ctccagactg gatctttaat ccgctgacta agaataacaa
     4081 tcaaagtacc taccatcaag gttacagcca gagaatgccc acttgggaag gaaaatccct
     4141 tctcctccac cagatgtaag atatctggcc gtgggcgctg gtagatattt ttaaaggtca
     4201 cgattaaaag acctgccaaa gccagatttc ccagcatgaa gaaactttct atcttccatc
     4261 gcttacgata aaagacaaaa gctgtaatga caacccaagt gataatcact gggatatcaa
     4321 tcagacgtgt gagggctcga aaaagaatag tcaaataatc tggtaagtct cctcgaatgg
     4381 cagtctgaat cgattggtca aaattgacca gcgtttcagg gtaaaatttg accatgtagc
     4441 caagaataac gaaaagtaaa agggcaaaac tgcccttcat taaaaatgtt tgtttatctc
     4501 tcataatgtt ttaaggttgg tttcaagaga acatacaaca accagaatga aacggaaaag
     4561 ataacacctt caatcaagtt aaaaggtaat accatggtca ttaggtagtt ggaaagtccc
     4621 aaaatttttc caatatcaaa gttagcaaac ttagcgtaca aaggaacagc ataaacatag
     4681 ttgagaacca acatggccaa ggttaaacca atagttccag ctagagagcc tagtaggaaa
     4741 cgaagggttg tccgttcctt tttccaaatc aaagcaaata cgatgacaaa aactcccaaa
     4801 gctacgatat tcatcggcaa accaatgtaa gtattcactc cttggctgtt aagaagcaac
     4861 ttcaagagtg agcgaagcaa gagcactcct agagcagcag gcaaatccat gaccaccaga
     4921 cccacaagga ctggcaagat actaaattcg atcttgagga aagatgccgc tggtaaaagc
     4981 ggaaaatcaa agtacatcag cacaaatgag atggctgata gaattgcaat ggtcgaaagt
     5041 cgacgtgtgt ttgtcataac aggttcctcc aattttctat aaaatcagaa gaagttggaa
     5101 aggattcctc tatctattct cactttttat atcccaaaag ttccctctta ctctattaaa
     5161 gaaaaacaaa gcaagtggtt acaatccggc tataaatcta tcaaaacaga caaagctatt
     5221 ctttcgtctt ctcccatcca gactatactg tcggttgtgg aatctcacca catcagcttg
     5281 cgctcgcgga cttctttaaa gggaaagaaa tagagatttt tcttttagac taggcgacaa
     5341 gctgtaggca taacttctgg ttaggcaaag cttgtcaaca aagtataaaa ggaaaagatc
     5401 atttcctagt taaagtcacc gccggtcggg aatcacaccc tgccctgaag actcttttat
     5461 cataacaaaa aacgcttgca agcgcaagca ttttgttcat tttcattttt tatttcaatt
     5521 tatccacttc ataggtatgg acgagctcaa gacctgcaaa gttctgctgg cgaagagcct
     5581 catagacaat catgcagacg gtattagaca cattgagact gcggacatgt tcatcattca
     5641 tgggaatacg gagagccttc tcaggatgtt ctcgcataaa atcttcaggt aagcccttgt
     5701 cttcacgtcc aaagagaaaa taatggtcct cgtcagtcga taaatccacc tcagaataga
     5761 ttttttccgc gaatttcgaa atcagataga gttttccctt catctgagat atgaaatctt
     5821 ctaaactctc gtaaaaataa atctctagct tatcccagta atccaatcca gcccgcttca
     5881 tcttgcggtc atcaatagga aagcccatag gcttgatgat gtggagggga gaattggtcg
     5941 cagcgcaagt acgcgcgata ttgcctgtat tttgtggaat ttgaggttca aataatacaa
     6001 tgtgatttgt catgacttgc ttcctttcac cattgcaaaa aaatagccac actgcccgga
     6061 gtcaagctca gcaaacagcg tggttaaggc atcgttaact tacctcacaa caggtttgaa
     6121 gtaaatcagc gaaactactt tcttagtata acactttcag aatcattgtc aatagaaatg
     6181 acttgatttt ttcaattttt tcaagctatt tccaagggtt gtaaaatcgt ccctgattct
     6241 gcaagataag tagtaaacta actactaaaa acaaggttgc caagagcaag gtaatatagt
     6301 ctcctttttt caaggcctga taactatacc atgtgcgttt tttctctttc ccaaagcggc
     6361 gaagctccat ggcagtcgca atggtatcaa tgcgttctag cgagctaaaa atcaagggcg
     6421 taataatgag cagattgcct ttgattcgtt gcataagaga agctttcttg gataattcca
     6481 tcccacgcgc ctcctgagac atcttgatag taaagaattc ttcctgcaaa tctggaatat
     6541 agcgcaaggt caggctgaca gaataagcaa tcttataggg cacaccaatt tgatttaaac
     6601 tggaagcaaa ctgactagga tgggttgtca tcaaaaagat aatagccaga ggaatggtgc
     6661 aaagatactt aatggccaaa tttagcagat aaaagagctc ctggctggtt agagtgtaga
     6721 caccgattcc ctgccaaatc acacttctct ctccataaag tccaacccca tactcgggag
     6781 aaaagagata gaccatcaaa acgtttaaaa cggcaaatac cgtcgcaaaa acggctacaa
     6841 aggaaatatc tttaaagcga atttctgata aatagaggag aaagactgaa aagatggcaa
     6901 tcagcaagag cattctggta tcatagctaa tcatggccgc caatgatacc agaatgaaaa
     6961 agagaagttt cccagctcct gacaagcgat gaatcacagt atctctatgc tggtaaccga
     7021 ttaatttagc ttgcatccct ctctcctttc tttgtaaaat gccgttaaat ccagtggatc
     7081 cacatctagt ttcttagcca agttaaagat ggaggtttct tttagattgg cttttactaa
     7141 cagctcagga tcgctcaaca gactggctgg aacagtatcg gcaatcaatt ctccatccac
     7201 catgacaagg acccggtctg aataatccag catcaattgc atatcatggg taatcatgac
     7261 aatggtatgc cctttttgat gtaactcttc gagaaattcc ataatctcag tatagttctt
     7321 ctgatcttga cctgcagtcg gttcatctag gagaataatt tcagctccta agaccaaaat
     7381 tgaagcaatg gtgacacgtt ttttctgacc aaatgacagg gcagaaatag gccaattacg
     7441 gaattcataa agtccacaga ttttcaaggt ttcatagact ctcgtttcaa tttccttctc
     7501 atccacacct cgcaaacgga gccctagagc cacctcatca aaaatcatat tggttgaaat
     7561 catttgatta ggattttgta gcacatagcc tactcgttcc gcccgctctg caacagaatc
     7621 gccttttata tcctgttttt cccaaagata gcgtccttcc gtctgaataa agctacttat
     7681 agccttggct agagttgatt tccctgctcc attttttccg acaatagcaa tcttttcacc
     7741 ctttttaata tctaaatgta gggattttaa aatcggtcta tcatcataag agaaagacac
     7801 gtcctctaat ttaaagagtg actgcaattc tggagtttct tttgccagtt cattctgcag
     7861 ctgaacctga ccttttgaga tagacatgtt atccagattc gctaactgtt cttccttgac
     7921 taagtccaca cctaattgac ggagagtcgt tagataaagg ggttctcgaa ttccattttg
     7981 agtcaataaa tcagtcgcaa gcaactggtc agggctccca ttaaaaagga tacgaccatc
     8041 gcttatcaag acaatccgat ccacagggcg atgcagaaca tcctccaaac ggtgctcgat
     8101 aatcagagtc gtcgtcccct cttccttatg aatctggtca atcaattcga taatatcctg
     8161 acctgacttg ggatctagat tggcgagtgg ctcatcaaac aagagaatcg gactttcatc
     8221 aatcaagaca ccagccagac tgactcgctg cttttgtcca cctgacaaat cctgaggacg
     8281 ctgatccagt aaaggaagaa ggtccagctt ttcagcccat ttataaacac gacctttcat
     8341 ctcatctagg gctgtcacat cattttccag agcaaacgcc aaatcttctg ccacagacaa
     8401 gccaataaac tgcccatctg tatcctgcaa aactgtgcta accagatgag acttatcata
     8461 gatgctcata tcaaaggcta cttgaccctt tatcaaaaat tctccatatg tctgaccctt
     8521 gtaaatattg ggaataatcc cattcaaaca ctgacccaag gtagatttac ctgacccaga
     8581 tggtccaaca attaagactt tctctccctt gtaaatggtc aagtctatcc cttgcaaggt
     8641 cggttcttgt tgtgtttcat accggaaaga gaaatccttc cactcaatta tagcttcttt
     8701 catcttactc tcttcattcg cttcttagac ttctatttta tcataaatca agcccttctt
     8761 gcagtctctt ctcttaaaat cttagcgcca aaaagattcc tatcctagct tacttgccta
     8821 actaatctat aaacatcgaa aaagactagt tgcccagcct tccccatcat tttatactct
     8881 tcgaaaatct cttcaaacca cgtcagcttc gccttgccgt aggtatggtt actgacttcg
     8941 tcagtttcat ctacaacctc aaaaccatgt tttgagctga cttcgtcagt ttcatctaca
     9001 acctcaaaac catgttttga gctgacttcg tcagttctat ccacaatctc aaaacactgt
     9061 tttgagcaac ctgcggctag cttcctagtt tgctctttga ttttcattga gtattagtcc
     9121 tttttcaaac ttcctgcacg agtttgggtt cctgcatagg caagtaagag aagagttcct
     9181 gcaatagcta cagatacacc attggcaatt cccgcaacaa tcccttgtgc aaatactttt
     9241 tctgccgctt cttgataaat cacaacatct ccaagtggtg ccaagacacc ccaaacaagg
     9301 gcatttgcaa gtagttgaat gaggttaaaa ataagaatat ctttccagtc aaaaacacca
     9361 ttgatcactc gaacgtactt tctaaaaagt cccacaacta gaccaaagag tccgctagcg
     9421 ataatccaag tccaccatag accataacca gcaagagagt ccttgattgc atgaccaatc
     9481 aacccgacaa gcaaaccgat aatcggtcca aaaataatag aaagtagcgc ttgtaccgca
     9541 tactgaagct ggatgcttgt atttggaaca ggggttggaa tgttgatcat cccgatgacg
     9601 acaaagaggg cagcgccaat tccgacagca acaacttgtt taattgtaaa tttgatttcc
     9661 atactattct cctattttat ccttctattt tctttatttc aatggtccaa gatgaaccga
     9721 cacctacatt ataggccttg gcaaaggaac cttggttgat agccaaacct aaacgataga
     9781 gagagttgat gtaaaggatg ggttgcccaa ttctcacatc tgcaaatgat ttgccataga
     9841 caacctgatt ttgatagacc agcatatcag catgatagat ggtcacttca aaacgatcac
     9901 caaattctgg ttccagcttg taaaattctt cccgtgtgat agaggtccaa agcgaaccga
     9961 aacgcacatc cagaatatca atggctccct tcaccagatg atcttctatg atggtcgcta
    10021 cgactggaag ctctacaatc tgttccacac tgagctctgg ccctacttcc tcaaaagtaa
    10081 tgtgaccact ggccagttta gcaccagtat aggcatagac atcacgaccg tggaaggtat
    10141 aagaatgctc tgtgttttga cgcctattgg ccacctcaga aatctcacga atggctacaa
    10201 tgccaacgtg tttcttgata aaggaaagcg tcccattatc tggcgtgaca atgtattgat
    10261 tttttgcagt cttggcaact acactcttac gtttcgaacc gacacctgga tcgacaaccg
    10321 atacaaacgt cgttccctca ggccagtaat ccaccgtctg aaagagacga tagctcccct
    10381 caaaaatatt ataaggcgtg atatcgtgcg tcaagtgatg gatttttaag gttggagact
    10441 cttctaaagc cactccaatc atagccgata ccgcaccatc aaccagacca aagtctgatt
    10501 gtaataccag taaattatta ttcattttat ctccttgtat atcctttatc ataccatatt
    10561 tcagagaaag tcgctaatag ctatttcaat tggttagggt atagttttac cttatagcga
    10621 attttcacta aaagctcttg ttattagcta gtaggtgcat tttttcttga aaaagggtat
    10681 gatagttaca tcatgaaaaa gtaggtttta ttatgaaaat tatccttgtc ggagggggaa
    10741 aagttggttt tgccctctgt cgctccttgg ttgcagaaaa gcatgatgtt ttgctgattg
    10801 agcacgacga agctgttctc aatcatattg tcagtcgctt tgatatcatt ggtatccttg
    10861 gtaacggggc cgattttgcc attcttgagc aagccagcgt ccaagattgt gatatcttta
    10921 tcgccctgac tgagcacgat gaagtcaaca tgattgcagc agttctagcc aagaaaatgg
    10981 gagctaaaga aactatcgtt cgggtgcgga accctgaata ttctaactct tatttcaagg
    11041 aaaagaatat tctcggtttt tctcttatcg ttaatcctga gctcttggct gcccgcgcta
    11101 tcgcgaatat cattgacttc cccaacgccc tgtctgtcga acgctttgct ggtggacgcg
    11161 ttagcctcat ggaatttgtc gtcaagtcca ccagcggtct ttgccaaatg cccatttctg
    11221 attttcgtaa aaaatttggt aatgtcattg tctgtgcgat agagagggat catcaaatta
    11281 tcattccaag cggtgacatg actgtacagg ataaagatag aatctttgtc actggtaacc
    11341 gtgtcgatat gatactcttc cataattatt ttaaatcacg cgccgtgaag agccttctca
    11401 tcgttggggc aggtagaatt gcctattatc tacttggtat tctcaaagat agtcgtatcg
    11461 atacaaaagt cattgaaatc aatcctgaaa tcgccagctt ctttagcgag aaattcccaa
    11521 atctctacat cgttcaagga gatggaaccg caaaagatat cctgctggaa gaaagtgctc
    11581 aacactatga tgccgttgcg actctaacag gtgtcgatga ggaaaatctg attacatcta
    11641 tgttccttga cagggtaggt gtacagaaaa atattactaa ggtcaatcgt accagtctcc
    11701 tcgagattat caatgcgcct gatttttcaa gtatcatcac acctaaaagc atcgctgtag
    11761 atacgattat gcactttatt cgtggtcgag ttaatgccca gtattcagac cttcaagcca
    11821 tgcaccatct agccaatggc caaattgaaa ccctgcaatt ccatatcaag gaagccaata
    11881 aaatgactgc caaacctctt tctcaactga aattgaaaaa aggggttctt attgcagcca
    11941 ttattcgaaa gggcaagact attttcccaa ctggggagga tatgttggaa gttggagaca
    12001 agctcctagt aacaaccttg ttgccaaaca tcaccaagat ttatgacttg attgcgaggt
    12061 aagaaatgaa taaaagtatg attcgttacc tcctttcaaa gttacttttg attgaagctg
    12121 ttctcctctt ggttcctgtg gctatcgctg tctattaccg tgaatcgagc caagtcttta
    12181 cagccctctt ttcgaccata gggattctcg tattgttagg cggttcaggg agtttacaga
    12241 agccaaaaaa tcaacggatt tatgccaagg agggagtctt gatcgttgcc ctctgttgga
    12301 tcctttggtc tttctttggt ggtctcccct ttgtcttttc tgggcaaatc cctagcgtta
    12361 ttgatgcctt ttttgaaatt agttctgggt ttacaactac tggagcaagt attttgaacg
    12421 acatttcggt tctcagccgt tccctcctct tctggcgaag ttttacccac ttgattggag
    12481 ggatgggagt gcttgttttt gcacttgcta ttatggacaa tgccaaaaat agccacctag
    12541 aggtgatgaa ggctgaggtt ccaggtcctg tttttggcaa ggttgtatcc aaactaaaaa
    12601 acactgccca gattctctat ctcctttatc tagctctctt ctccctcttt gtcatcatct
    12661 attatctagc tggtatgcct ctctacgata gttttgtcat tgctatgggg acagcgggaa
    12721 ctggaggctt taccgtctat aacgacggaa ttgcccacta tggcagctca ctgattacct
    12781 atctggtcag tatcggagtt ctggtttttg gggtaaattt caacctctac tactacctca
    12841 tgctccgtcg catcaaggcc ttctttggtg acgaagaact tcgggcttac ttggtcattg
    12901 tactggtttc tacaggcttg attagcctca acaccctcta cctctaccca gggttttcaa
    12961 agagctttga aatgaccttc ttccaggttt ccaacatcat tacaacaact ggttttggat
    13021 atggagatat taccaactgg cccctcttct cccagtttat cctccttttc ctcatggcaa
    13081 tcggtggctc tgctggatca actgcaggtg gactcaagat tattcgaggc ctcatccttt
    13141 caaaaattgc caaaaaccaa attttgtcaa tcctatcgcc ccaccgtgtt ttgaccctcc
    13201 atgttaatca aacggtgatt gacaaagata cccagcataa gattctcaag tactttgtca
    13261 tctatgctat gattttgcta gcgcttatct ttattgtcag cctagatagc aatgattttc
    13321 tggtcgtgac cagcgctgtc tttagctgtt tcaataatat cgggcctatt ctaggaacca
    13381 cttctagttt ctcaatcttt agtcccatct caaaaattct cctatccttt gcaatgattg
    13441 cagggcgctt ggagatttac ccaatcctcc ttctctttat gaagagaact tggtctaaga
    13501 gataaaatac aaaatccctc tttttacaat tgtgaaagag ggattcttat attaaaaaag
    13561 ggggaacgtt tttaaatcca agcctatctg taagcaaaat ctttactcaa ataaaagatg
    13621 gaaaaactta ctacacagct tggtaatgct ccaatacaaa ttgtaaggct ttacttggac
    13681 tctttgttaa atgaatatat tccattcctc tggtagcaac tatctgaata cctaatcttt
    13741 ctgcatccac cttcatctct ctataataac tccgaacttg tggggccaga ataattaaat
    13801 cataaacacc cataatatca taatgagctc cgtacgctcc tgaattcgca atcactctaa
    13861 cttctgctaa ttgagcgccc tcgttaattg cattggctaa ttgcgcactt gttccagacc
    13921 ctgcacaaag aaccagtact ttcaactcct tactttttat tacatctatc tcaccaggca
    13981 ttatttcact tgtatcctcc tctaaaataa tatcatttga gcttgcaata tcttctttca
    14041 ccagtaactg tctatcatac gctctacaga atggcaaata aatcaatatg tcgacaacta
    14101 acatcaaaga taaaaataca aaggagataa gttgaaaatt cgttccaatt aacaatccca
    14161 agggaccagg aaaggcccaa ggtaactgga tataaaatcc attcattcca aagaaatcaa
    14221 taaatacctt tcctagaaat acattcactg gtggagtcat caaaaaaggg acaaaaagat
    14281 agggattcaa aataacaggc ataccaaata gaagaggttc atttaccgca aataaaacag
    14341 gagtaattgt agctttacct accgatttta attgtttaga ccgcataaag aaaatcaaaa
    14401 taaatggtac aacaaaggta gcccccgttc ctccaatagc agcgatataa ttcccgaaat
    14461 taggtgttaa agaatgataa gggaactgcc catttgcaag aagttgagca ttctcttcca
    14521 tattgctcag ttgtaaagct gtaacagcag gtaagacaat agatggtcca tgaattccaa
    14581 caaaccaaag caaagcacac ataaaccaaa tcaacatcat agcaggatat gattctgccc
    14641 ccttaaaaat aggagtcaat agttgttgaa atacttgggc aaaaggaaca tctaaactaa
    14701 aacgagatac aatatctaac aaaccactaa taagtaaaac aaaagaaaaa ggaaaaatat
    14761 ctctaaaagc ttgtgatata gccccaggaa cttccttagg taaatgaata gtaatgtctc
    14821 gcttaataca gaatcggtaa acatttacag taataaaggc actgacaaac gaagttatca
    14881 atccttgaga tcctaaatag tttgtcgaca aacttgtaga tcccgttttc tcatcaacta
    14941 caagcgttac agttaataca agatagccac atatggccgc caacattgca gaagtatcat
    15001 ttattacctt tccaggaggc atttttctat taacatttcc agccaatgac tttgcaactg
    15061 tccctgaaac aataataccc aaaactccca tggttagcat atacactttc ctcatccagg
    15121 caatagctgg actcggtaac tctactccga aatttttagg aatcataata ataatcatca
    15181 aaaagcttga aaacataatt aaaggcatag tagcaataaa tccatcttta atagccatca
    15241 tataattatt agtagaaatt ttttcaaatt tcccttgttg cttttcaatg gcagcgacta
    15301 acttatccat aaaaactcct cctgtaaaaa atttaagctc atagttaaaa tatattaaac
    15361 atcagtcaaa tcgctaacct taattccgtt ttagcccttt tacccaaaga gcacttttct
    15421 taatatatct cttctgtgtt tcataatcaa caaagaaaag accatatcgc ttattgtagc
    15481 cattcgccca agaaaattga tcttgcaaag accatataaa atagccttga atattgactc
    15541 ctctatctcg agcctctaaa actttgtgta aatgttgctc aatataacgg attctcttac
    15601 tgtcatcaat aatatcattc tcgccctctg gcttaacttc tttaagggct gtaccatttt
    15661 cagttaaata aatgaccgga tgttgaggat attcttcttt gatacgaagc aacatatcaa
    15721 ataacccttg aggataaata ttccaatccc aatctgttgt cggaatacca ggttttttta
    15781 caaattcacc tagagcattt aatttaaaag aagactgtcc ttttattcct gttgaattaa
    15841 attcttgtct attttcacct ttgtattcac gaataaattc tgaacgataa taattcatcc
    15901 caaacatcgt attaagagct gcagctctct tcataatttc taaatcacca tcttcaataa
    15961 taaagctaga attattagct tccaatattt gattcaagtg aaaaagcgtg tcctcactgt
    16021 agtagcccaa aaaagttcca tctaatagaa atttattatt ataaacatca taccgtttag
    16081 ctgccaaaat attttctttt tgcccatcaa taggatagcc tggctttaaa gcatgaatac
    16141 aacctacctt tccctcaatc cctaattgat gaaattcgag gactgcaaga gcatgcgcca
    16201 acaacatatt atgattcgct tgaattgctt cagataattg aaaatgatga tttggaggaa
    16261 actgacctcc tatatattga cctgcagcaa gagacatcag ttcattgatt gtaaaccaat
    16321 gcttgacttc tgtaaattct tggaaacaaa agcgagcata tcgtatgaaa cgatcaatat
    16381 tctctctatt caaccaatcc cctgtttcta acattttctg aggcgaatca aaatgatgta
    16441 aagaaacaaa cggaatcaca ttatgtttta agcaagactg aaagactcta tggtaataac
    16501 gaacagctaa tatattaggc tccccatcta tatcaggaaa tatacgaacc caagaaatag
    16561 ataaacgcaa agcctgcaaa ccatgttctg ccgccaaagc tatatcctct tcgtaacgat
    16621 aataaaaatc actagccgga tctggtaaga acggactgtt ttcttgcaaa taaacatccc
    16681 acatatttgt tcctttgcca tctaccctag ttgcaccctc tacttgataa gcagcggtag
    16741 cagctcctaa taaaaactcc ttcggtaagg ctttaacctg taaattttcc atgactaatc
    16801 ctcattgcaa accaatatgt tcttcaatct ttcgaattcg ttcatattca tcaataaaaa
    16861 acttgacctg atcatacaat agcatcgttg tcatcaaggt atcttgacca tgaatcatga
    16921 taaaactcac ttcaaaatta tctccagatg cctcctccgc taataaatta gtctgttctc
    16981 gatgggcaga cactattgag tcgtttgcag actcaaccaa ttctcttgcc tgttcaaaat
    17041 atccatctct cgcaaacgcc aaagcatcca tcaaatctga ccttgcatct cctgcgtaag
    17101 cgacaattgt aaaaccaagc atatttattt cttcttttga tttcatcttt cttcctcctc
    17161 aatcttgatc catcttttac acatcattat taaaatgact tcgctacgat ataaatagaa
    17221 agtcgaccct aaatatctta aaaatcctta aatttcataa tatttaggat caatcttctc
    17281 tgaatacctt ggttcatttt ctatacttat gatttataag tggaaaccag cgtaattttt
    17341 tgctacttca attacacgat aaacatccaa attagattgg aatagcagtt cgtgcccacg
    17401 gatataattc aacacatttc tccattcatc ttgagcttgc cttgtttttc cggttgcttg
    17461 aagatataaa gccttcgcca tcttaacaac atattcacga tgataactga gctgtttcca
    17521 ttcatccttt tgactattta ataacttaga aatattttcc tcaataattg gcaaaaaatt
    17581 atcagctaga ttgtaagcta tataataatg attcgctaaa acatcatttt gacgactgcc
    17641 aattgcatta aaataatcac atgaggaata actggataat ttttctaaat attcaacaac
    17701 agactgccaa tttgacccat acaaagcaga aaagtattct tcaatcaatt cttcataact
    17761 tcttgtcttc ttccagagca tttcccccat aacataatta ggaaaattat gagggaatcc
    17821 ggcacgtaat tcttgacacg aaatgtaacc gttcaaatgt aggttagaaa gataagatac
    17881 atctctgtaa atggtttgac taattttcat atagcctaaa tcgccataat gagcacgccc
    17941 taaaggatag tcatatacga aactatctcc tttaaatgtt ttttgccact caaaaagata
    18001 agataaattt tcctcaagag aattcggaag tataatttta ttacgcagat aaggtttagg
    18061 cgtaggtatg gaattgtcaa aatctacatc tgcataactc atttcaaatg ttcttgtaat
    18121 cggtgcaaac atcatggtaa agcgttcagg attatctaat ttttctttct gaggcgccca
    18181 tagcaactca tggtaaagca gaaaacaaat ctttgtatct aatccctcac tcgttaaagc
    18241 cctatccaat tgattgagaa tacgaatata ctgatccgaa accaattctt gtctacagtt
    18301 ttcgcattca caaatattat tacgagcatc cgacaaccat acatgtaagt agttaacatc
    18361 aggtcttttc ttggcataat ccttgataat ttctaccatc ttatcagcta catctggatt
    18421 tgaaaaatcc aggctggtta aaatcggagc cgtattaaac aattctcgtt tcccgtttat
    18481 ttcagcgaca tagggtttct tctcctctga aatactaaga cctgattccc agccaaattt
    18541 tgaagagtaa cctaaaactt cacctgtcca tccatgacca acacgatgat gaataagacc
    18601 tcttttttgc aattctttat ccaacctatc actcaattct tgtactaatt catttgaaaa
    18661 ttgttcttta tttagatatg gattaaattc atgttcatac caacgtttca aaaaagagta
    18721 aggattttca aactggatga aaaaactgtt catcccaatc ttaggtagcc aatcaatgaa
    18781 atctagtata ttttcaaatg aatccgctcc ctctatacat actcccctat gatagtaact
    18841 ggctgtttca tctatagata gctgtttatc taaaaaatct tcaaatcgta actctggaac
    18901 aaagtcatgt ctgcgccctg gtctagtata cacaacccca aattcatgaa acattcggta
    18961 aattccaata agtaaaccta caattgtatt agactcaatt cttcccttac ccttgtctaa
    19021 tgagataaga acatggtctt ttctgtcctc tttattgact aatcctacaa aaatatccgc
    19081 ttccttctcc tcgacaagct ctatgttagt cttaaagacc cgattcatat agtaacttaa
    19141 ttcctctaag gcaaagctag aataagtatt tttctgttga tcaaatactc tgataaccat
    19201 cttatttccc tccaacagta gctttttctt tttcaagatt gcgtttatct gcaattttga
    19261 agaatggata ataaattgct acagaaacca aaatcaaaac aacttgtaat agagctcctc
    19321 gccaactagc ccctgttgca aggaagcctc ctataatcgg tggcattgtc cacggaagga
    19381 ttacacctgt tgtatagggt actaatccta cagccattga tacataggta atcaaggcat
    19441 tgattgtagg agtagcaata aacggaatca gcataatcgg atttaaaact gttggaaacg
    19501 taaatagaat agctgtattg atattaaaaa tagacggtat aatagctagc ttacctaatg
    19561 ttttattcgc acgactctta ctaaatagga agagacaaat cgcaagacca atagtcgctc
    19621 caccgccacc aataaataca aataaatctt taaacggtaa tgtaataatg tgttggagtg
    19681 tttgtcctgc agccacagct tcttggtttt ctgtagtaaa ttgtaaccaa actggatcta
    19741 caaaagcatt taaaacttgt cccccattaa ctccacagaa ccaaaagaat gagtttacaa
    19801 taacacatag aatcatacct ggaagcgttc ctgcaattaa cttaagcggt gttccaacaa
    19861 ttgcacctag gagtccgttg agacctcctg caactcctgc cgcttcaaga ccttttaaga
    19921 ccaaagccca caaaacaaaa gtagtaaagc caggtaaaag agctgaaaat gatttactta
    19981 ccacatctgg gacaccactt ggcattttta tcgtaattcc gcgctggata aacatacgat
    20041 aaatctctgc tgtaaccaat ccaatagtaa tcgccataaa caaagaagat gcattcaggc
    20101 tagaaaatgg tattgcgccg ccaaataact gcttgacctg ctccccattt ttatcataaa
    20161 ccatactcga aaaacgaggt gccatcaata caaaactgga taaggctatg atccctgccg
    20221 acggaccatc tgtaccatat ccttccgaca acctgtaggc aataccgaaa caagcgacca
    20281 aggagataat cgctactgtt gactgattca tagttgtcaa gatacttagc aatccaacac
    20341 tattcagcca atttgtaaaa tcctcttgag gccaactgat tagaataaga aataaagatc
    20401 cgactaaaat caaaggcatt gccatcataa atccttcacg aatagctttc aaatgccgtt
    20461 ggttgccaac tttttcacca aacggagcga cttttgtttc taaaaacgct aaaatattgt
    20521 tcatcaccgg aactcctttt tttatttttt ataaattaaa tataccacta acagaaagcg
    20581 ttgtcaatat attttttgtt gtttttacaa tatttttttc tttgtataga ataataattt
    20641 taattgattt tttcttatgt ttaatataaa ataaaccaaa ggaggaaaca aatgattaca
    20701 aaacaatcag cctttttaca aaatagagcc actatactgg agtttctata tcgaaatcca
    20761 gcaacatccc gtacagatat tgttaatgaa acaggactta ctccagcaac aacgactaac
    20821 atcataaaag agctaagcga acagtccctc atatacgaaa ctggtgatga atttagcgag
    20881 ttttccggat ctggaagacg tagaaaaact atttctatca cagataatat tccgtatgtt
    20941 gttggaggta ttgaaataaa cgtcctaggt atctttctca gtctatgtga cttacaaggg
    21001 aaaactcttt tcgagacaga aattttgaat gaagattatc ctatttcaga aatcaattcc
    21061 accattacca atatgataaa aacagctata gagtacgtcc ctttggaaac aaaattactt
    21121 ggatttggct tatcaatacc tggacattat aacaaagact ccggaagtat cattacaaac
    21181 aaccccatat gggaatcttt taatttatta aatgtaatta aaagattcaa ttttcctttt
    21241 attgtaaaaa ataatatcga ttgtatggct ataggacaat acctttttaa tccacacaat
    21301 acccccgata actttatttt cctacacgct ggattaggta tttacacttc ctttttcaca
    21361 aaagaaaaaa taggagcctc taaaaatcct tatatcggag aaattggaca caccattgtc
    21421 gaattgaatg ggcaatattg tgaatgcgga aaaaaaggtt gtttacaaac atatatttcg
    21481 gatgcttggt taatcaaaca cgcccaatta ttatttaaaa attcccaact aactgtacta
    21541 aaaagccttg taaagactga aaaagacatt catttagaca cccttttaac ggcttataat
    21601 ttaggcgact ccgctttacg tcaacaaatt gataaaggag tcaatttatt agccacttct
    21661 attgcaaatc tcctcctcat caatcctgct gataaaatct atatcaacag tcaattgctt
    21721 aattatcaac ctttcactca tgaagtcagg gataaaatcc aagaccagct ccacttcgtt
    21781 ccctttactc gtaatataga aattgaaatt ttaccttaca acaaacatcg tggaagtata
    21841 ggagcttgtg cattagctat cgtcgctttt ttcatagaac atagcaatgt attacaagat
    21901 attatttcac cttaatatat tagaaatcta tagacctgtt taaatcaact ataacctgta
    21961 gtagatatct cgtatttaga caatatgaaa acaagacgac ttccatatag gaaaccgcct
    22021 tctctctatg ttgagtgatt tatattaaaa taacttttct tctagctgca ttttattatt
    22081 ataaaaacat tcatcataac cctgaactta aataacgatt tttattcaag atacatactc
    22141 ctagaataaa ctttatatga aattctcatt tttgttttta caattctcct tagttaaatc
    22201 ttgtttaata tatgttttac atatagtatt tagcgccaca tagtactgaa ctctctccaa
    22261 aaacggttat tcctctttga atagggcgtt atcacaagaa aagcatctcc acgtttcaac
    22321 ttcatatggc tcaaaaacaa tcaattgatg ctaaaacctg tacctagatg tttcggttca
    22381 taaaaccatg aaactgtaaa agtggatgaa attgatagcg atagtcaaat caagaggcat
    22441 cataactcta aaaagtcaca atatataagt tcatcctcgg aaaaatatca ttctaattgt
    22501 tgaaatgcct acatgaaaag aaacgtcaaa tgctcatgaa acaacgaata caggtatcaa
    22561 aactatgaca aaacaagtcc ctaaatttac taaagacact gctcaacttt acacctgtaa
    22621 atggttgttg tataataaag ttacaaagat gtacgaccac actgttgtaa atcatagtgt
    22681 tcgcgaatat attactgata gcatttctac aaatacaatt gaagagactg gataggcttt
    22741 aaacgaaata tgtcagtgtt ttggcattcc tagtcttcat atcatttaaa gaattgtata
    22801 gacaaaattt ttttccaata cagacactcg taacaactgc ttcatttttc taccaacata
    22861 tttaggaaca ggataagata gaagaagatt aatccatagc tcagttctat accaatctaa
    22921 gacaaataag ctaaaaaaac gattgataat aagcaaatag attccaaatt ttctctatct
    22981 gctcatttta ataaacaata ctagtgtaac tatccttcca gtcagaaact tgtcaaatca
    23041 caccgaaaac tcttctaaaa tttatctcgt taggcaatca agcaaaaact cgacgatagt
    23101 acaaacatta tcatacagaa ttgactgcct aaattatata ctttagtaag gttttcggat
    23161 aagaaaaaag gttcatttta catttctaaa cattcttttc taagatgaaa aacagaattt
    23221 ttcgattgtg atttaaagca acaagaagat tttcagtatc atcctataga tacgagctaa
    23281 ttaagaaaaa ctacattttt gaatataaac tacaataata taaactaaat tttataggag
    23341 gaagacaatg gattggtacg attatatgat acaggcatcc aaacaatcac aattcaacgc
    23401 aagccattgg tttcgctatt tgcgaaaagt tatttttgaa gactattctt atttaacaaa
    23461 ccaagatgta gaaaagttgc tagactccaa agaactaacc cgttttcaaa aaattagctt
    23521 gaagtatgcc tttcaagagc atactccaac tcataaatat gtgatttcat taaataaacc
    23581 tgctaagtta accaatgttc aaaaattgat ggagaaatac aaacatggat aaaatgaaac
    23641 cggtcttcca agccctaaat aagaaattaa ttcaggaaaa tctgacttta acaattatgg
    23701 ttatgtctta gaatatcatg gtttacgtgc cacacaagat gttgatgctt tttatgacca
    23761 aaatcagaaa atcaatgaaa ttattgctcg tgtaggaaaa cagtttaatc ttaacatcca
    23821 cgaagagtta tggctcaaca atcatgttgc aaagcaaata tgaacaaaca acctccctta
    23881 agtttatgcg aatccttgta ctcatttgag aatctgaccg ttttagtagt ccctattgag
    23941 tatgtgttag gaatgaagat gatgagtatc agagagcagg atttgcaaga tattggtgca
    24001 attattaaat acaaaaactt tcattcacca ttcgatacct ttaaatattt aaaagacatg
    24061 ggatttgata ctattgacct ctctgttcta ttagaaggtt ttagttatgc ttatggtatg
    24121 gattggttag aaaaattttt taaagaaaat caagataaac ttcgcgaatt ttattaacag
    24181 tatcaaacaa gatgattgtc aattagccag tcgtcttatt tttatgatga aatgaaatga
    24241 aataaaatcg gaacaaatca cttagagaat tccagtcaat ttctagcact gtttgagccg
    24301 tttcagcgga ctatagtaaa atgaaataag aataggacaa atcgatcagg acagtcaaat
    24361 cgatttctaa gaatgtttta gaagtaaagg tgtactattc tagtttcaat ctattatatt
    24421 tcagattcgg tccactgttc cttcccaaaa acattgcata ttgcgccgaa ggaattgctc
    24481 acactatccc aagcacaaaa ccctcagtcg attgaccgag ggttcctttt cattattcaa
    24541 aaacttgatt agctcaatgc atccaaggca tcatccattg aaagaacttc gtggaagaca
    24601 cgttgtgtca attcagtttt ttgttctgga gtgaggtatt tagtatttac acagtatcca
    24661 gagatacgta cgataacgtc ttcacctgac atgatttttt cgtaaacatc gttcaagtcc
    24721 ataacgttca agttaacgtg ttgtccaccg ttttcgaagt aaccatcaag gattgttacc
    24781 aagttatcaa cttgttcatc acgagtctta ccaagagcgc gaggtgatac ttgtgtagtc
    24841 aatgagatac cgtcagctgc ataactaaag tcaaggctag aaagtgagtt caagttttgc
    24901 aaccaaccac ctttagcttt gttagatggg ttagcacctg gtgagaagaa ttcaagttta
    24961 gacaagttca cagaaccatc ttcgttgagg tatacacctt tgtgaactgg tgagttacca
    25021 gtttgtttag agtaagcaac gttagatgtg attgtcaaaa gtgatactgt agcttctgcg
    25081 tctttgtata gtttgtggct acgtagacga gttgtgtaag cttcgatcaa ccattctgcc
    25141 aattcgtttg aacgtgggtc atcttcaccc cagcgtgggt agtcaccgat tgtttcgtaa
    25201 tcgtagatgt agccatcttc gtcacggatt ggtttaactg tagcgtattt gatagctgac
    25261 aatgtatcaa cagtgttagc aaatccacag ataccgaatc ccatgttcgc acgttgttta
    25321 gttggcaaga aggccatttg aacagcttcg tagttgtacc tatcagtcat gtagtggatg
    25381 atgttcaagg catctacgta agtgtcagtc aaccagtcaa gagatttttc aaagttcgct
    25441 ttaactgatt caaattcaag aacttcgtca cggattggtt cgatatcaaa tactttgtag
    25501 tctttgtgaa catcgtcgta accaccattc aaaccagtaa gaagggcttt aagaacgttt
    25561 acacgagcac cgaagtactg gatgttgtgg cgttgttctt cattttctgg atcaagtgga
    25621 gacacacagc atgagataca gctcatttca ccatatccgt ctttagccat tgttgttaca
    25681 ccttcgtatt ggatagaaga gtgtttgtgg ctcatgtgca tacagtagcg acggaagttg
    25741 tatggcaatt tgtcagtcca aagaactgtc aagtttggtt ctggtgagtt accgatgttg
    25801 tcaagagtgt tcaagaaacg gtagtccatc ttagtaacac ggtgacgacc gtcgttaccc
    25861 ataccagcca tagaagttgt gataaaggtt gggtcacctg agtacaattg gtcataagct
    25921 ttggtacgag caaatttaac tgtacgaagt ttcataacga aatcatcaac gaattcttgg
    25981 atttctgatt cagtaaatgt accacgagca aggtcacgtt ctgcaaagat gtccaatacg
    26041 attggtacac gacctagaga tgtagcagca ccgttaatca cacggcagac agccatgaaa
    26101 gcaatgttaa cccattggat tgcttctttc acgttcatcg ctggtttgcg aacatcaacc
    26161 ccgtaaaggt cacccaggcg aacaacttgt tgcaatgctt ggtattgaag gtttacttct
    26221 tcacgaagac ggattgtttc ttcatcgatt tctttgattg cattccagtc gtttactttt
    26281 tcttgcatca agtagtctgc accgtaaaga gcaagacgtg cgtaaacacc gatgatacgt
    26341 ccgcgtgagt atgcatctgg aagaccagtt acagtgtgtg catgacgagc gcgacgaata
    26401 tttgaagtgt aggcacggaa aataccgtcg ttaactgttg ttacatattt agtgaagatt
    26461 tcgtgaacag ctgggtctgg ttcgtatcca ttttctttta aagtagtttc agccatacgg
    26521 ataccacctt ttggcatgaa gttcaatttg aagagttcat cattttggat accaaagata
    26581 acttcatttt ctttgtcgat aaatccagca gggatatcag cgatagatgt tggacgagtg
    26641 tccattggga aacgagtttc ttcgtagtgt gctttagttt cttctacaat tttcttgatg
    26701 tgaagtgaac gctctgttgg tcctgcaagg aagctttcgt ctccatcata aggtgtgtag
    26761 ttagcttgta caaagcgtga tacacttgct ttttctttcc aatctacgcc tttgaagcct
    26821 tcccaagctt tgtcaaaaat atcttgtgct tcaacaactg tcttaacaac catattaatg
    26881 tcctcttttt tctagtaaca gtcatctgtt acattcataa gacaagtata ccataaagta
    26941 accgatttca acaagtgcaa aatccctatt tttacacttt cttttctaaa acagtctata
    27001 ttttatttca aactgtatta tatttttgaa aaaaataaag tccttttttc tttttttcag
    27061 aaaaaagggt ataataaaag aaaataagca gtaacactca atggaaatcg aaaagcaaac
    27121 taggaagcta gccgcaggct gctcaaagca ctgctttgag gttgtaaata gaactgacga
    27181 agtcagctca aaatactgtt ttgaggttgc agatggaagc tgacgtggtt tgaagagatt
    27241 ttcgaagagt ataaaaaggt gctaggcatg ttgatttttc ctttgttaaa tgatttgtca
    27301 agaaaaatca tccatattga catggatgcc ttttttgctg cagtggaaat cagggataat
    27361 cctaaactca gaggaaaacc tgtcattatt ggaagcgacc ctcggcaaac aggtggacgg
    27421 ggagtcgttt ctacctgtag ttatgaggca agagcttttg gtgtccattc tgccatgagt
    27481 tccaaggaag cttatgaacg ttgtccccag gctgtcttta tctcagggaa ttatgagaaa
    27541 tacaagtctg tgggactccg gattcgagct atttttaagc gctatacaga tttgattgaa
    27601 cccatgagta ttgacgaagc ctatttggat gtgacagaaa ataaactcgg tatcaagtca
    27661 gcggtcaaaa ttgctcgcct cattcaaaaa gatatctggc aagaactcca tctaactgct
    27721 tctgccggta tttcctataa caagttctta gctaaaatgg cgagtgatta tcaaaaacct
    27781 catggtttga cagtgattct acctgaacag gctgaggatt ttctcaaaca aatggatatt
    27841 tccaaatttc atggagtagg aaaaaagaca gtagaacgtc ttcatcaaat gggtgttttt
    27901 actggtgctg atttacttga agttcctgag gtgaccctaa tagaccgttt tggtagacta
    27961 ggctatgatc tgtatcgaaa ggctcgtggc attcacaact ctccagtcaa atccaatcgc
    28021 atccgtaaat caatcggcaa ggagaaaacc tacgggaaga ttctccgtgc tgaggaagat
    28081 atcaaaaaag agctgactct tctatcagaa aaagtcgctc tcaatctaca tcaacaagaa
    28141 aaagctggaa aaattgtcat tttgaaaatc cgctacgagg acttttcaac tcttaccaaa
    28201 cgaaaaagta ttgctcaaaa aacacaagat gctagtcaga taagccaaat agccctgcaa
    28261 ctctatgaag aattaagtga gaaagaaaga ggtgtccgcc tattggggat taccatgact
    28321 ggattttaaa gctcaatgaa aatcaaagag caaactagga ggctagccgc aggctgctca
    28381 aaacactgtt ttgaggttgc agataaagct gacgcggttt gaagagattt tctaagagta
    28441 taaaaccttg aagagttgcc ccttcaaggt ttttcttata caaataaacg gacaaaactg
    28501 taaagtagca aaacactacc aagcacgata cggtatttac caaaaagggt gaagtcgtgt
    28561 tttttcacat agctggtcaa gaagcgaata gccaccatgc tgaccgcaaa agctactccc
    28621 atcgcgacca agagcaaaaa caattgccca aagctcaaga gttctccggc tttcacaaat
    28681 ttgaaaatct ttaaggcact agctccaaac ataacgggaa tcccaagata gaaggtaaat
    28741 tctgtcacaa ctgaacgact ggttccattt aacaaaccac cgacaatcgt tgcacctgaa
    28801 cggctagtcc ctggtaaaag agcaagaact tggaagagtc cgatatagaa agcggtcgta
    28861 taaggaagct tgtccaactc tgttacactt ggctcgatag cacgcgcttt attgcgcttt
    28921 tccaaataga tgaaggcaac cccgtagata atcaacatga gagcaactga aaccatgtta
    28981 tggaagtggg tatcaaacca atcatcaaat ttaaagacac caagtaaagg caaagtggca
    29041 atcaagacct tcaaccatag tctccaagtc ttacgaactt cctgtttgtc cttagtcggt
    29101 ttaaaaggat tgagcttgtt aaaataaatc accataactg ctaaaatagc accaagctga
    29161 atcacgacat taaacatgga cataaaggct tcattttgat tttggtattg gataaactcc
    29221 tctgctaaaa tcaagtgacc tgtactggaa atcggcaacc attccgtaat tccttcaaca
    29281 atcccgaaga agatagattt taaaatttca ataagataca tatatttctc cttatattat
    29341 atttaacgca gttagctact gccaaagccc aagtggtata cttggaataa gccactgtgg
    29401 attagttcat ttcctccaat cgcttgacga tttccgaaag gctctaacca cagctttttg
    29461 tttcaattgg taatgagttt gataacgcat agacgtttta tctcgtctaa ggtaacaagg
    29521 caaaaagttc gtggataacc aagtagactt gaaatactat atctggaggt actcccatgc
    29581 tttatgtggg cattgatgtc gctaaaaata aacacgatgt tacagccttg aatgttcgag
    29641 gaaaactgtt cttaaaccac tcactttttc aaataataaa gctggttttg aactcttaga
    29701 tctgtctttt cgacagctca accaagactg tctcatcgct cttaaacttc tttctgaccc
    29761 caatcgtgaa caatttcaac acgataatcg gcaagtagaa ctaaaaatac tggctagaca
    29821 tattcatcgt ctcaagaaaa aacagtctga ttggaaagta caatacactc gttgtcttga
    29881 tatcatcttt cctgagttgg ataaaatcgt tggaaagcat tcagaatata cctaccaact
    29941 cttgacacgc taccctaatc ctcagaaaag gcttgaggca agatttgata agctgataga
    30001 aattaagcga ttgaccgctt ctaaaattca gaatatcctc tcagtcgcac ctcgttctat
    30061 cggaacaaca tctcctgctc gtgaattcga aatcatcgaa atcatcaaac attacaagag
    30121 gctcattgac aaggcggaaa catgtgtcaa tgacttgatg gctgagttca actcggccat
    30181 cacgacggtt actgggattg ggggtcgttt aggggcggtc attttagccg agattcgaaa
    30241 tattcatgcc tttgataatc ctgctcaatt acaagctttc gctggactgg attcttctat
    30301 ttatcagtca ggtcagattg atttagctgg aagaatggtc aaacggggtt cccctcatct
    30361 gcggtgggca ctcatacaag ctgccaaagc atgcgctcgc ttttcacctg cttttaaggc
    30421 ctatcttaag actaagttag aataaggaaa acattacaat gtagccatca tccaccttgc
    30481 aaaaaaactt atccgaaccc tgttttatat cctaaaaaag agctgccatt tgacgaacaa
    30541 aaagtgatgt aatttctgaa attaacacag gattaccttt gaaaaaatgg actttttcaa
    30601 gggctttttg ccgtgctcta aaatcatcgc caaatcatca aattgaaata agaaatagtt
    30661 tagataaaac tattgacttt tcttatagtt tgtctttctt ctatcttcca ttatatcata
    30721 ctttttcagt ctatggtagc tctctttaaa aggctccgat actgccgcca cctccgcctc
    30781 cagagaagcc accgccagaa cttccacttc cagaagatac ggagtaggta cttgctgtat
    30841 ttgcgacact agcataatgg ctcatttgcg cgcttgaatg ataaaacata ctgtgccagc
    30901 cataagctac atagagattg atatctggat tttcaacttg aatctgatga accttcatca
    30961 aatgactaac cttgtccgca tagccaaata aggttgcata gaccaagaga cgattccaaa
    31021 caacaatact ttccaattca gccttatcca gtcgtgcaat ctcacgtaac atattttcaa
    31081 aactggtcca gagatagtag acctcagctc ccgcttcatt tagaacacca tcacgattat
    31141 ctagtcgaag cttccaataa tagaaaacag acaaaactaa ccctagaaaa ccaagtattg
    31201 gcaaagggag gtaaagatag ccatgaacgt ctaaactgta caagaacaaa ccaaatccga
    31261 taaatagggg caagatagtc aagacaccca tacccacttg caaagccttt tccaaaccag
    31321 ttaaaggacg atagtaatct gggagccccc agaaggaaac tcgttttctc actccttctt
    31381 gcatctggtt caatacctct tcaaaagaag atttgagttg aagccctctt gcttgaatcc
    31441 gtttttcatc agaaactttg gctctacgat aaagactatc agataccttg taatccgcaa
    31501 acaaattgga aagagtttct tcttttttac ctgaaaaagc tagatttagg cagtctttct
    31561 caaagcttga caaaccatct tcttttacta gcctcaaacc aactgcatct ccttctgaaa
    31621 tgatagagac attcccacgg tctatcacat ctagcaaggt agcttgaata agttgatcaa
    31681 aggtgaattt tccagctccc ttgaccaagg gactcacttc ctccaaggag gtcgagtaga
    31741 ctgcttctga taaaaccata ggctctaatt ccattggggg ttcatagaga cgatgatttt
    31801 tagcatattt gattgaagga gcggtctttc ttctatagat aaaatagaaa cagatgctca
    31861 ataacaagga gatggaaagg atcgaaggga agatccaagt aagtagttgt ttactttgat
    31921 ctttttcttt aacgatcgaa tcctctatct tattaaactc ttctaaacga ttccctttca
    31981 agccctgatc cctagcgcta gcaaaatcag ttcgcggcca gtaggcatgc aattcaactc
    32041 cacgcttagc cggaagattg tctaaacgaa tagtataatc aaggttactc ttttcaaccg
    32101 ttctctctct aaaaagtttc cctgtatgga aaaagagttt ctcagccccc ttgtctcccc
    32161 ttacatgaaa ttcaaacttt tcaatagact ctgaactatc tgtcagaggt taccaattta
    32221 attcagcgat atcatcataa aggaaaagta aattttttaa gttccagacg aggtcaactt
    32281 caactatgtc gccctcctga cctggattat agactctcac agtataacca tccgcttctt
    32341 ctgttacttc gctagtcaca tctgctagtt ctgcaccgtt tttcgcggcc tgaatctttg
    32401 gatgagggtc aatgtcaaac ccgctaggca tcttaccagc acgtccaagt cccacgattt
    32461 ggcccttaaa gtcctcctca aactggtaaa ctatcttctg tctaaactct gccgtattgt
    32521 ctgcatgaat atacaaatcc ccttgataag agtttatctt gaaatcaatg gcaaaaacag
    32581 agagtggaag aaggcaaaac aagcctaaca ccagtaagaa aaaagttttt ttcatcaact
    32641 aagccccctt tttatctttc ttatcattat agcatttttt cgtaagtaaa ggcttactta
    32701 tgttgaaaaa ttacgcattt ttcgataaaa taggagacag ttatttttta atagatcaga
    32761 ataggagaaa tcatgagaaa aatatactta tctattttca caagtctctt gctgatgcta
    32821 ggacttgtca atgttgctca agccgatgaa tatttacgca tcggtatgga agcagcatat
    32881 gctcccttta actggaccca ggatgatgat agcaacggag ctgtcaaaat cgatgggacc
    32941 aatcagtatg ccaacggata cgatgttcaa atcgccaaga aaatcgctaa ggacttaggt
    33001 aaagaacctt tggttgttaa aaccaagtgg gaaggtctag tccctgccct tacttctggt
    33061 aagattgaca tgattatcgc aggtatgagt ccaactgcag aacgcaaaca agaaattgcc
    33121 ttttcgagca gttactatac tagcgaacca gttttgcttg tcaaaaaaga ttctgcctac
    33181 gcaagtgcta aatctttgga tgactttaac ggtgcaaaaa tcacttctca acaaggggtc
    33241 tacctttata acttgattgc acaaatccca ggtgctaaaa aagaaacagc catgggagac
    33301 ttcgctcaaa tgcgacaagc tcttgaggct ggtgtcattg atgcttatgt ttctgaacgt
    33361 ccagaagcac tgactgctga agctgcgaac tctaagttca agatgattca agtagaacct
    33421 ggtttcaaaa ctggggaaga agatacagct atcgctatcg ggcttcgtaa aaatgacaat
    33481 cgtattagcc aaatcaatgc cagcattgaa accatttcaa aagatgacca agttgccttg
    33541 atggatcgta tgatcaagga acaacctgcc gaagctacaa caactgaaga gactagcagt
    33601 agtttcttta gccaagttgc taaaattctt tctgaaaact ggcaacaact cttgcgtggt
    33661 gctggtatca ctcttttaat ctctatcgtc ggaaccatca taggtctcat tattggactt
    33721 gccattggtg tcttccgtac tgctcctctc tctgaaaaca aagtcattta cggcctacaa
    33781 aaactagtcg gctgggttct caatgtctac attgaaattt tccgtggtac gccaatgatt
    33841 gttcaatcga tggttatcta ctatggaact gcccaagctt tcgggatcaa ccttgaccgt
    33901 acactggctg ctatcttcat cgtttcaatc aataccggtg cctacatgac tgaaatcgtc
    33961 cgtggtggta tcctagcagt tgacaaggga caatttgaag ctgcgactgc tcttggtatg
    34021 acccataacc agaccatgcg taagattgtc ctacctcagg tagtccgtaa catcctacct
    34081 gcaactggta atgaatttgt catcaatatc aaagatacat ctgtattgaa cgttatctct
    34141 gttgtcgaac tttatttctc aggaaatacc gtggcaacac aaacctatca atacttccag
    34201 acatttacaa tcatcgccgt gatttacttt gtcctcacct tcaccgtaac acgtatccta
    34261 cgctttatcg agcgcagaat ggacatggat acctacacta caggtgctaa ccaaatgcaa
    34321 acggaggatt tgaaataatg acacaagcaa tccttgaaat taaacacctc aaaaaatcct
    34381 atggacaaaa cgaagtgcta aaagacattt cactcactgt ccacaaggga gaggtcatct
    34441 ctatcatcgg aagctctgga agcggaaaat cgaccttcct acgctccatt aacctacttg
    34501 aaacaccaac tgatggacaa atcctttatc atggacaaaa cgtcctcgaa aaaggctatg
    34561 acctcacgca ataccgtgaa aagttgggga tggttttcca atcctttaac ctctttgaaa
    34621 atctcaatgt tcttgaaaac acaatcgtcg ctcagacaac tgtcctaaaa cgcgaacgca
    34681 cagaagctga aaagattgcc aaagaaaacc tggaaaaggt cggcatggga gaacgctact
    34741 ggcaagccaa accaaaacaa ctctcaggtg gtcaaaaaca acgtgtggcc atcgctcgtg
    34801 ccctctccat gaatccggac gctattctct ttgatgaacc aacatcagct ctcgatccag
    34861 aaatggttgg agaagtcctc aaaatcatgc aggacctggc tcaggaaggc ttgaccatga
    34921 ttgtcgtaac ccatgaaatg gaatttgccc gtgatgtctc tcaccgtgtt atctttatgg
    34981 ataagggcgt gatcgctgaa gaaggtaaac cagaagacct cttcaccaat cctaaagaag
    35041 accgaacaaa agagttcctt caacgctatc tcaaataaaa agaaaaggct gcatcaaccg
    35101 tgcagtcttt ttgctgtcct caaaatgaaa aggcagattc tctatctcta ctacttgaag
    35161 gtcaagaatc cgccgttatc caaagattaa tcttcaaatt tttcataatt tttttcatag
    35221 aaatcaatta ggcctagggc tgtttcaaat gaaatatttt ttacttttgc acgaccctgc
    35281 gccagagcaa tgatagacat ttcacgcgca ttcgtttctt tagagatacg gtagcctgtg
    35341 attttcttat cacgaaccca gccaacaact gattctactt tttcaaagtt tgacttagcc
    35401 atgtttttct cctattttct tctttataat atttaattgt atacgttttt actacttttg
    35461 tcaataaaaa aaacatatat tcaacgaaaa taattagttc agaattttct tacttccttt
    35521 ttaaatccga taatatatag gttttgctag cctctacacc ataaatattt gctaataaaa
    35581 atactgtatt attaccctct taaggtgcga aattattcaa gttcttaaaa aatgcttctc
    35641 tatttccgta agatagctta tttcttcgat ttaagaactg aagatgatag ttctaacctt
    35701 tagaaatcca agccttcact agctcaaaaa ctcctatctt tcttcctcaa taaatcatct
    35761 gagagatagg tattccttat gtccttcaaa ttcatcactc ctttggatat tgaggtgcta
    35821 gcctagaagc agacacccga ttatctaaac catccttaat agagatgaca agacagataa
    35881 gctcagttaa ttacttatat atctttttaa tatagtatat ttgtcaacaa aaagaaatca
    35941 aatcatacct tatactcaat gaaaatcaaa gagcaaacta ggaagctagc cgcaagttgc
    36001 tcaaaacact gttttgaggt tgcagataga gctaacgtgg tttgaagaga ttttcgaaga
    36061 gtattaaaga cagtgtgact atgcttgtca atagatagga aatcaaaatg aaggtatgat
    36121 atttttttat tagtcctcag acaagcatat tataaagcgt ttcattacca tttaagttaa
    36181 tataagctgg atcaaaacct tccattctac gaatcaaacc tgcataatca tgcttatctg
    36241 ctaaagcgat cccaattaat actgggcctg ttcccttgct agctcgtttg atatactcaa
    36301 aacgtgtgat atcatcattt ggccccagga tatcatttac aaactcacgc aaagctcctg
    36361 gacgttgtgg gaaattgacc acaaagtaat gtttgatacc atcataaatc aaggcacgct
    36421 cttccatttc tggcatacgg ttgatatcat tatttcctcc agaaatgata caacaaatgg
    36481 ttttcccctt aatatattca gctaaaacct ctaaagaggc gatactagcc gctccagcag
    36541 gttctgcgac tatcccttgc ttagagtaaa ggtcaatcaa ggtttcagaa atcaatccct
    36601 catcgacacc taccaaagtt ttaatatgtt gacgagttgc ttcataggtc aactgaccta
    36661 ccttttgcac agcaatccca tcagcaaatt tatcaatttc cttgagtttt actggacctc
    36721 cagcctcaaa ggcagctttc atggaacgcg ctccattcgc ctctactccg ataacctcaa
    36781 tctctggact tgtttccttg atataggtag aaaccccggc aatgagaccg ccaccaccaa
    36841 caggaaccaa gacagcatca aaatcaatcg attcttttcg agcttcttct aaaatctcat
    36901 aagcaactgt tccttgacct gcttgaacat gagcatcatc aaaaggatca ataaaggtac
    36961 gattttcaga gactgtaaat tcttgagctg ctttggctga ggcatcaaag gtatctccaa
    37021 ctagtttaat agttacaaaa tccccaccaa aaaagcgaac ctgaccaatc ttttgttgtg
    37081 gcgtagtaat gggcataaag atagtagcag gaattttcat ttcattacaa gtataggcta
    37141 ctccctgcgc atgatttccc gcagaagcgc agactacccc acgttcacgt tcttccttgc
    37201 tgagctggga aatggcataa taggcaccac gaattttaaa ggagcgaaca cgctgggcat
    37261 tttctttttt caaataaatc ttagcaccat acttctccga taaataatga tcgtaatcca
    37321 gtggagtatt cacaaccaca ccgttcaaga ccttgtgagc cttgattata tcttttgaac
    37381 ttaacatata aaacctcttt ttctataaaa aacagtcact aatgactgtt ttttagttat
    37441 tcatctccga aaacttcagt aacactttca tggtatttat gagccatacg aatcgaaaac
    37501 tctttaggaa gttcataacg atttgtggaa atccaatcat ataaccattc taccaaactt
    37561 tcttcactat cattacgatg gaagataaaa tagtttaaaa tttctttata ttctacatca
    37621 ttcaaatcca acgtgtctac aaattgacta cgaggaacaa aattagaatc taattttctt
    37681 agttgatctg tcaattctcc caattgtaaa tccaactgaa aaaatagttt ttgcagttgc
    37741 ctatcagttt catcacatcc tgtcttgata gaaaacaaat tggtaatatt catatgattt
    37801 caacccctaa ataagtacat ctaatatccc acgaatagct gaagctaaag ttttcgcagt
    37861 ccagtcattt aaacccgatt ctttcaatcc agatgcaata atttgctcta tttgatataa
    37921 accgccatct atcgtctcat ttattctgcg aagaggacct tcaatatagg ataaagcttc
    37981 tccgaatcta acagctgatt taggtcctaa tgatttacca actatatttg ttaatgtttt
    38041 ttatttctca aaatccattt cgctatataa cgagcagatt tagtcgcacc agctctctca
    38101 aacggagaat gaatttcaag accttcttct ctagctattt gtgccaattt gaagaattgt
    38161 tcaaagttca tatgcaacct accatcttca aaattagcat catcataaat gttaattaca
    38221 gcattttcat catacgacca atctgcttta ttttggtctg ctagcactat taccgacggt
    38281 gtaattacat tatacaacta gaaccaataa taacgatgca atttttctca taatcttttt
    38341 cataaaatca tctccagcgc tatatatttc taattagtta tagattttga atgcatcatc
    38401 gtcgttttta ccaacgaatg gcattgcttt acgcaattct gcaccaactt tttcaatttc
    38461 aaggttagct gcttgttcac ggtaagcagt caattttgga cgtccagctt tatagtcatt
    38521 tacaaagtca tttgcaaatt taccattttg gatgtctgcc aagacagcct tcatattttc
    38581 tttaacttgt tcagtgatta cacgtggacc tgatacatag tcaccgtatt cagcagtgtt
    38641 tgaaatagat tgacgcattt tcttgaatcc accttcgtag atcaagtcaa cgatcaattt
    38701 catttcgtga agaacttcaa agtaagccaa ttctggagcg taacctgctt ctgtcaagac
    38761 ttcgaaacct gcttcgataa gggcagtcaa accaccacaa agtacagctt gttcaccaaa
    38821 caaatcttct tcagtttctt ctttgtaagt tgtttcaaga agacctacac gagccgctcc
    38881 aacaccttta caccagtcca tagcaatgtt tttagcattt cctgttgcat cttggtatac
    38941 tgcataaaga gctggaacac caaatccttc ttcgtaagta cgacgtacca agtgtcctgg
    39001 tcctttagga gcacacatga agacatctac atccgcagga actttgataa attcaaagtg
    39061 gatgttgaaa ccatgggcaa atccaactgc gtttccagct tccaagtttg gagcgatttc
    39121 tgcttcgtac aattcttgtt gaatttcgtc tggcgccaag atcatgataa catcagccaa
    39181 cttagtagct tctgctactg tgtaagcatc aaatccatct tcttttgctt tatcaaaaga
    39241 tttacctgga cgtacaccga taataacgtc acgacctgaa tcacgcaagt tttgagcatg
    39301 cgcatgccct tgtgaaccat aaccgataac ggcgattttt ttaccgtcaa gtgctgctac
    39361 tttaacatct ttttcatatt ccatttgaac tgtcatagct tttctctctt ttctattatt
    39421 tattgccttt taggctggtt taataaattt aagttggatt tttaatcgcg ggtaaatcca
    39481 gttgcacccg ttcgagcaat attgcgaata ccgtatgggc gaatgactcg caatagggct
    39541 tcgctctttt ctgcatttcc cgtcatctga atggtaatcg agcttggcgc tacgtctact
    39601 actgttgcac ggaaaggttg aataatcgct aaaatctcag ctctcttctc agctggcgct
    39661 gacatcttaa ccaaaatcac ctcgcgctcc aaatgaggct tgtctgtaat atctcgaatg
    39721 cgaatcacat caatctgacg attgagctgt ttgatgattt gctccacttc atcatgagaa
    39781 gcaacatcaa taataatagt gatacgcgat acattcggat cttctgttgc tccaacagag
    39841 atgctttcaa tattaacctg acgacgagat aggacacctg taaagcgatt gaggactcct
    39901 gaacgatttt gtagttttgc tgttaacatt ctacgcatgg aactgcaccc ccaacatctc
    39961 atgattactc ttaccagccg gtaccattgg taacacctgt tccttacgag aaatatctac
    40021 ctcaattagc ataggaacat cctcagtgat gacttcaagg tcttgagcca aggtctcagg
    40081 attgtcaaac ttatagtttt taataccata agcctgcgcc atcaattgga aatcaggaag
    40141 ggtatcaaag accgactctg atgttctgcc ttcatagaag gattcctgcc actggcgaac
    40201 cattccaagt gaatgattgt tcagcataac caccttgatt ggcaccttgt aaatattcaa
    40261 aatagccaac tcctggttgg tcatttggaa accaccatcc ccaacaaaca agactacttc
    40321 cttatctggg ttagcaattt tagcaccgat tgctgctgga attccaaagc ccattgttcc
    40381 caaaccacct gaagtcacta actgacgttc attttggtag ggataatact gagctgtcca
    40441 catttggtgt tgaccaacgt ctgttaccac aatggcatct ccattcgtca attcaccaat
    40501 tcgttcaata actgcttgcg gttgaaccac acgctctttc ttatcataag aacgaacacg
    40561 attcttgtct ttagtgactt tctcaatcca cttttcagtg ttgttgtgaa ctgttggttc
    40621 tgctagcaac atttgcaagg ccttcttagc atctccaact acaggaatgt ctgcactgat
    40681 aatcttgcca atctcagctg ggtcaatatc aatgtgggca accttagcat tcttagcgaa
    40741 agtcttagga ttccccgtca aacggtcatc gaaacgagaa ccaatactaa tcataaagtc
    40801 cgcttccgtc atagcaatat ttgctgcgaa tgacccgtgc atgcctccca ttccaagaaa
    40861 gagtgggtga ctcgttgcaa tcgttccttg tcccaaaaga ctggttacca ctggaatttg
    40921 atagcgttct gcaaattcat ttagttccgt agcagcctca gcataactaa ttccaccacc
    40981 agctaacaag actggctttt tagccttgga caattgcttc aagattttct tgatttgcat
    41041 atcattcggc tcaagagtcg gctgataact tggtaaattc acttctggtg aataaatgaa
    41101 gtctgtttct aaagcagata tgtcttttgg taggtcaatt acaactggcc ctggacggcc
    41161 tgtagttgcg atatggacag cttccgtaat gatacgcgga atatcagctg tctcacgaac
    41221 ttggtaattg tacttagtga ttggcatggt aattcccacg atgtctgcct cctgaaaggc
    41281 atccttccca atccctgctc gcgccacctg acctgtaaag accaaaaggg gaacgctatc
    41341 gctcatggca tccgcaatcc ctgtaatggc atttgttgct cctggtccac tagtgacgac
    41401 ggcaacaccc aactttccag ttgatttggc ataaccttca gcttcatgca aacaaccttg
    41461 ctcatggcgc cctagaatgt ggcgaatgcc tttaaaatta tatatcgcat cataaaaagg
    41521 caaaaccgca ccaccaggat aaccaaagat ggtatcaact cctaaatcac gaagtgtttc
    41581 caaaactagg tccgaccccg tcttaggaga ttctaaactg attttctcca ttattcccct
    41641 ttctttcctc ttaaaaataa cttgttacta tcataccatt ttttcaaaat ttttcaagat
    41701 aaaagaagaa attttctgaa ttttctattt taacgtttat ttatgaatgt tatttttgtt
    41761 tttattaaaa agataccgat ttcatttact aaaaagaaaa aagaactgat ttctcagtcc
    41821 ttcattaatc ttattccaca ctaaataggt atgggtaaac aggttgttga ccttggtgaa
    41881 tctcgacttc aacgtcttcg aattcttcta cgatttcttg agcgatttca ttggcaagtt
    41941 cttcgcttcc gtcttcacct acatagaagg ttacgatttc actgtcttca tccaacatat
    42001 gtttcaaggt ttcagtcaat gtttggtgca tatcagggtt tgacacaaga atttttccat
    42061 ccaccatacc taaattatcg ttttcatgga tttctaagcc atcgatcgtt gtatcacgca
    42121 cggctgttgt gacgcttccg ctaacgacat cgctaagagc agctgtcata cgctcttggt
    42181 tttcttcaat ggacttgctt ggatcaaagg caagaagact tgtcatacct tgaggaagag
    42241 tgcgagcctc taccactacc gctggttgct ccaaaacttc tgccgcagat tgagctgcca
    42301 tgaagatgtt cttgttgttt ggcaagaaga tgatgttacg ggcattaacc tgttcaacag
    42361 ccttgataaa gtcttctgtt gaagggttca tggtttgacc gccttcgata acataatcca
    42421 cgccttgaga acagaagata tctgctagac ctttaccagc caccacagca atcaaagcat
    42481 actctttttc ttcagccgac ttgataactt gagtagcttc tttctcaacc tgtgcttcgt
    42541 gttggttacg catattgtca acttttacct tgaccaagct accatatttg agaccttctt
    42601 gcataacaag tcctggatct tctgtatgaa catggacttt gacaatttca tcatcgttaa
    42661 caacaaggag agaatctcca agctcatcca agtagttacg gaattcatcg tagtcaaaat
    42721 ctttagcata ggttggacct tgcttaagag ctaccatgat ttcagtacag taaccaaacg
    42781 tgatgtcctc agtcgctacg tgaccagcta cagacttatg atgctctaca ttgatcatct
    42841 cactcatgtt ggcaggagtc gctacaaagt cctcagatgc aatatattcg ccagtaaggg
    42901 ctgaaaggaa accttcgtag atgaagacca atccttgacc acctgagtcc acaacgccaa
    42961 cttctttcaa tactggaagc atgtctggtg ttttagctag agctgtttta gcaccttcca
    43021 aggctgcgcg catgacttca acagcgtcat ctgtttgctc agcttttttc ttagcaccga
    43081 tagcagctcc acgagaaact gttaaaatcg ttccttcaac aggtttcatc actgccttat
    43141 aggcaacttc cacacctgat tggaaggcca gagccaagtc ttgacctgtt aactcgtctt
    43201 tatccttgat agcttgggaa aatccacaga aaagctgaga cgtaatcact cctgagttcc
    43261 cacgcgcacc catcaaaagc cctttggcaa gaatgctcgc tacttctcca actgtagaag
    43321 ctggcttgtc tgcaacttct ttagcaccat tttcaatggt cattcccata tttgtcccag
    43381 tatctccatc tggaactgga aagacgttta atgaattgac atattcagct tgcttattca
    43441 agcgagttga tgcagcctgc accatttctt gaaataagct agtagtaatt tttgacacgg
    43501 ttattctcct acaactttga tattttgaat gtagacattt acagtctgag cagtaattcc
    43561 aagctggttt tccaagctaa aggcaacacg ctcttgaatg ttttttgaca cttcactaat
    43621 ctttgttccg tagcttaaca cggtatatac atcaactgca atactgccat cttcggctgc
    43681 ctttacgacg acacctttag aataattttc cttacctagc agggcttgga aattatcttt
    43741 gagggcattt ttactagcca taccgaccac accaaaaatc tcagttgctg ctccacctac
    43801 gacggttgca atcacttcat ctgttagttc gatttgacca tcttttgtat taatttttac
    43861 agtcatcctt tttacctcaa ctagttgata ctctatttta tcatatttca gcccaagtgt
    43921 aaaagcagaa tgatgtatca gcggatattt actctatttt tcaaacgatg ttatacccac
    43981 aataaaagaa aaaagaccct aaggtctcct ttgcttttat tattaaacgc gttcaacttt
    44041 acctgatttc aaagcacgag ctgaagccca aactttttta ggtttaccat cgataagaac
    44101 agtaactttt tgaaggtttg gttttacggc acgttttgtt tggttcatcg cgtgtgaacg
    44161 gttgtttcct gatacagtct tacgacctgt aaagtaacat actttagcca ttgtgttttc
    44221 ctcctattag atctaatata gcggatgtgc tagcaccaca taccgtacta tgttatcaca
    44281 ctttcttgtt ttttgcaagg gaattggaag attttttatt tgtgtcttaa atcaggtctt
    44341 gcgtgacatt ttctgctctc cacatgccat tgttgattaa cagaacacca gaattaaaat
    44401 tatgtgtata aaaatcatct ctaactgcag ctaagggtat agccgtcaag tccaaatccc
    44461 acagctcatc tatcgatttt cttacaacaa tatctgaatc caaatacagt acacgagact
    44521 cgcttacata ctttggaata aaatacctaa aaaaagccgc atatgaaagt ccctcaaagg
    44581 ggagacgata acctttcaga atactactgt caatctgaac attcacaatc tcactattca
    44641 aagtctctag tcttttttcc atcaattgga accattctcg cggaaggtca tcattaaaaa
    44701 cataaaactt aagattataa tgatgaacac aaagagattt tattgttgtt tcaactttat
    44761 ccatataagc attatctgca cctaagacaa tcgctttttt ctcttctttc actctttatc
    44821 ttatttcttt ttattcccat catattattc ccatcatatg tttctacgta accattattt
    44881 tcgcctattc gttcgtaaaa ccataccagt ggagatttta gatgaagtcc cattacggtt
    44941 tacaattttt acattacgac acggagtttt acaaatcgat ttcatttgcc aaacgtagtt
    45001 agtgaggcag ttagctagtt cgccaaatag cgactagcgt ccaacaattt ggaactttag
    45061 ttccaattgt tggtactgag tcacatcttc tcctctaact ctacgtctgg atacttgtca
    45121 gcaaaccagc ggagggcaaa gtcattttca aagagaaaga ctggttggtc aaaacggtct
    45181 ttggctaaga tattgcgact tgacgacatc cgttcatcca agtcctcagg cttgatccaa
    45241 cgaacggtct ttttacccat tgggctcatg accacttccg cattgtattc gccttccatg
    45301 cggtgtttaa agacttcaaa ctggagttga ccaacagctc ccagcatgta ctcgcctgtt
    45361 tggtaattct tataaagctg aacggctcct tcttgcacca attgctcaat ccccttgtgg
    45421 aaggattttt gcttcataac attcttagca gaaactttca tgaaaatttc aggagtaaag
    45481 gttggcagtg gttcaaattc aaacttgttt ttaccaactg tcaaggtatc cccaacttga
    45541 taagtaccgg tatcgtaaac cccgataata tcacctgcaa cggcattggt cacattctca
    45601 cgactttccg ccataaactg agtaacatta gacagtttag cccccttacc agtacgaggg
    45661 agattaacac tcatgccgcg ctcaaattcg ccagatacga tacggacaaa ggcaatacgg
    45721 tcacggtgac gagggtccat gttggcttgg attttaaaga caaagcctga gaaatccttg
    45781 tcataaggat ccacaatttc accgtctgtt ttcttgtgac catgtggttc tggagcaaac
    45841 ttgaggaagg tttcaaggaa ggtctgcaca ccaaagtttg tcagggctga accgaaaaag
    45901 acaggcgtca attctccagc cagaatagct tcctctgaaa actcattccc ggcttcattt
    45961 aaaagctcaa tatcatcctt gacttgctcg tagaaaggat tgctaccaaa aagtttgtcc
    46021 ccatcttcta gactggcaaa acgctcatcc cctttataaa gctctaaacg ttggttatag
    46081 aggtcataca agccctcaaa ggctttcccc atcccgatag gccagttcat agggtagcta
    46141 gcaatgccca agatttcttc caattcttgt aagagatcca aaggctcacg accgtcacgg
    46201 tccagcttgt tcataaaggt aaagactgga atgccacgat gtttcacaac ctcaaacaat
    46261 ttcttggttt gagcctcgat ccccttggca gagtccacga ccatgaccgc agcatccacc
    46321 gccatcaagg tacgataggt atcttctgag aagtcctcgt gccctggcgt gtctaagata
    46381 ttcacgcgct tgccgtcgta gtcaaattgc ataacagatg aagtaacaga aatcccacgt
    46441 tgcttctcga tatccatcca atcagattta gcaaaagtcc ctgttttctt cccttttacc
    46501 gtaccagcct cacgaatttc acccccaaag tagagtaact gctcagtgat ggttgttttc
    46561 cccgcgtccg ggtgggagat aatggcaaag gtacgacgtt tcttaatttc ttcttgaata
    46621 ttcataagtt ctctttcttt gattctctat ttttcttgtt tcaatagctg agaatgattt
    46681 ttacattgga ttttaccatt cctttcaaca ctccattata tcggatttta gcattttttt
    46741 caatttctat ttcttttcac ttccccctcc cttatttata ggaaaatatg gtaaaataga
    46801 acagactaaa aatcatcatt tcacgaaagg atgcaagatg aaaattacgc aagaagaggt
    46861 aacacacgtt gccaatcttt caaaattaag attctctgaa aaagaaactg ctgcctttgc
    46921 gaccaccttg tctaagattg ttgacatggt tgaattgctg ggcgaagttg acacaactgg
    46981 tgtcgcacct actacgacta tggctgaccg caagactgta ctccgccctg atgtggccga
    47041 agaaggaaca gaccgtgatc gcttgtttaa aaacgtacct gaaaaagaca actactatat
    47101 caaggtgcca gctatcctag acgatggagg agatgcctaa tgacttttaa caataaaact
    47161 attgaagagt tgcacaatct ccttgtctct aaggaaattt ctgcaacaga attgacccaa
    47221 acaacacttg aaaatatcaa gtctcgtgag gaagctctca attcatttgt caccatcgct
    47281 gaggagcaag ctcttgttca agctaaagcc attgatgaag ctggaattga tgctgacaat
    47341 gtcctttcag gaattccact tgctgttaag gataacatct ctacagacgg tattctcaca
    47401 actgctgcct caaaaatgct ctacaactat gagccaatct ttgatgcgac agctgttgcc
    47461 aatgcaaaaa ccaagggcat gattgtcgtt ggaaagacca acatggacga atttgctatg
    47521 ggtggttcag gtgaaacttc acactacgga gcaactaaaa acgcttggga ccacagcaag
    47581 gttcctggtg ggtcatcaag tggttctgcc gcagctgtag cctcaggaca agttcgcttg
    47641 tcacttggtt ctgatactgg tggttccatc cgccaacctg ctgccttcaa cggaatcgtt
    47701 ggtctcaaac caacctacgg aacagtttca cgtttcggtc tcattgcctt tggtagctca
    47761 ttagaccaga ttggaccttt tgctcctact gttaaggaaa atgccctctt gctcaacgct
    47821 attgccagcg aagatgctaa agactctact tctgctcctg tccgcatcgc cgactttact
    47881 tcaaaaatcg gccaagacat caagggtatg aaaatcgctt tgcctaagga atacctcggt
    47941 gaaggaatta acccagaggt taaggaaacc attctaaatg ccgctaaaca ctttgaaaaa
    48001 ttgggtgcta ttgtcgaaga agtcagcctt cctcactcta aatacggagt tgccgtatac
    48061 tacatcatcg cttcatcaga agcttcatca aacttgcaac gcttcgacgg tatccgttac
    48121 ggctatcgcg cagaagatgc aaccaacctt gatgaaatct atgtaaacag ccgaagccaa
    48181 ggttttggtg aagaagtgaa gcgccgtatc atgctgggta ctttcagtct ttcatcaggt
    48241 tactacgatg cctactataa gaaggctgga caggtccgta cacttatcat tcaagatttc
    48301 gaaaaagtct tcgcggatta cgatttgatt ttgggtccaa ctgctccaag tgttgcctat
    48361 gacttggatt ctctcaacca tgacccagtt gccatgtact tagccgacct attgaccata
    48421 cctgtaaact tggcaggact gcctggaatt tcgattcctg ctggattctc tcaaggtcta
    48481 cctgtcggac tccaattgat tggtcccaag tactctgagg aaaccattta ccaagctgct
    48541 gctgcttttg aagcaacaac agactaccac aaacaacaac ccgtgatttt tggaggtgac
    48601 aactaatgaa ctttgaaaca gtcatcggac ttgaagtcca cgtagagctc aacaccaatt
    48661 caaaaatctt ctcacctact tctgcccact ttggaaatga ccaaaatgcc aacactaacg
    48721 tgattgactg gtctttccca ggagttctac cagttctcaa taaaggggtt gttgatgccg
    48781 gtatcaaggc tgctcttgcc ctcaacatgg acatccacaa aaagatgcac tttgaccgca
    48841 agaactactt ctatcctgat aaccccaaag cctaccaaat ttctcaattt gatgaaccaa
    48901 tcggatataa tggctggatt gaagtcaaac tagaagacgg tacgaccaag aaaatcggta
    48961 tcgaacgtgc ccacctagag gaagacgctg gtaaaaacac ccatggtaca gatggctact
    49021 cttatgttga cctcaaccgc caaggggttc ccttgattga gattgtatct gaggcagata
    49081 tgcgttcgcc agaggaggcc tatgcttatc tgacagccct caaggaagtt atccagtacg
    49141 ctggcatttc tgacgttaag atggaagaag gttctatgcg tgtggatgcc aacatctccc
    49201 ttcgccctta tggtcaagaa aaattcggta ccaagactga attgaagaac ctcaactcct
    49261 tctcaaacgt tcgtaaaggt cttgaatacg aagtccaacg ccaggctgaa attcttcgct
    49321 caggtggtca aatccgccaa gaaacacgcc gttacgatga agcgaataaa gcaaccattc
    49381 tcatgcgtgt caaggaaggg gctgcagact accgctactt cccagaacca gacctacccc
    49441 tctttgaaat ttctgacgag tggattgagg aaatgcggac tgagttgcca gagtttccaa
    49501 aagaacgtcg tgcgcgttat gtatctgacc ttggtttatc agactacgat gctagtcagt
    49561 tgactgctaa taaagtcact tctgatttct ttgaaaaagc tgttgctcta gatggtgatg
    49621 ccaaacaagt ctctaactgg ctccaagggg aagtcgctca gttcttgaat gctgaaggta
    49681 aaacactgga acaaatcgaa ttgacaccag aaaacttggt tgaaatgatt gccatcatcg
    49741 aagacggtac tatttcatct aagattgcca agaaagtctt tgtccatcta gctaaaaatg
    49801 gcggtggcgc gcgtgaatac gtggaaaaag caggtatggt tcaaatttca gatccagcta
    49861 tcttgatccc aatcatccac caagtctttg ccgataacga agctgctgtt gccgacttca
    49921 agtcaggcaa acgtaacgcc gacaaggcct ttacaggatt ccttatgaag gcaaccaaag
    49981 gccaagccaa cccacaagtt gcccttaaac tacttgcaca ggaattggcg aagttgaaag
    50041 aaaactagac agaacaaaac cagccctaag gttggttttt tcttctctac caactcccaa
    50101 taactatttt ggctttattt ccagagtatt ttatggtaaa atgaagagta ataatattta
    50161 ttaaagaggt aaaaacatga ttgaagcaag taaattaaaa gctggtatga cctttgaaac
    50221 agctgacggc aaattgattc gcgttttgga agctagtcac cacaaaccag gtaaaggaaa
    50281 cacgatcatg cgtatgaaat tgcgtgatgt ccgtactggt tctacatttg acacaagcta
    50341 ccgtccagag gaaaaatttg aacaagctat tatcgagact gtcccagctc aatacttgta
    50401 caaaatggat gacacagcat acttcatgaa tacagaaact tacgaccaat acgaaatccc
    50461 tgtagtcaat gttgaaaacg aattgcttta catccttgaa aactctgatg tgaaaatcca
    50521 attctacgga actgaagtga tcggtgtcac cgttcctact actgttgagt tgacagttgc
    50581 tgaaactcaa ccatctatca aaggtgctac tgttacaggt tctggtaaac cagcaacgat
    50641 ggaaactgga cttgtcgtaa acgttccaga cttcatcgaa gcaggacaaa aactcgttat
    50701 caacactgca gaaggaactt acgtttctcg tgcctaatct ctagaaagag gtcattctat
    50761 gggaattgaa gaacaacttg gcgaaatcgt tatcgcccca cgtgtacttg aaaaaatcat
    50821 tgctatcgct actgcaaagg tagagggtgt tcactctttt tcaaacagat cagtgtctga
    50881 taccctttca aaactttcac tcggccgtgg catttatctt aaaaacgtgg acgaagaact
    50941 cacagcagat atctatctct accttgagta cggagtaaaa gttcctaagg tagcggttgc
    51001 tatccagaaa gctgtcaaag atgccgtccg taatatggct gatgtagaac tcgctgctat
    51061 caatattcac gttgcaggta tcgtcccaga taaaacacca aaaccagaat tgaaagatct
    51121 atttgacgag gacttcctca atgactagtc cactattaga atctagacgc caactccgta
    51181 aatgcgcttt tcaagctctc atgagccttg agttcggtac ggatgtcgaa actgcttgtc
    51241 gtttcgccta tactcatgat cgtgaagata cggatgtaca acttccagcc tttttgatag
    51301 acctcgtttc tggtgttcaa gctaaaaagg aagaactaga taagcaaatc actcagcatt
    51361 taaaagcagg ttggaccatt gaacgcttaa cgctcgtgga gagaaacctc cttcgcttgg
    51421 gagtctttga aatcacttca tttgacactc ctcagctggt tgctgttaat gaagctatcg
    51481 agcttgcaaa ggacttctcc gatcaaaaat ctgcccgttt tatcaatgga ctgctcagcc
    51541 agtttgtaac agaagaacaa taaaaagaaa aagtgtttga tagatatcaa acactttttt
    51601 ctttgcctcc cactatctaa aaaaatgata atagatataa ttgtaaacaa aaatccagat
    51661 aggttttgca tgattgagaa agttaaaaaa actatggcag agaatcgtta atctcagatt
    51721 gtcggtagaa cgataaacaa gggcaaaaaa gaaaccaatc agactataat ataataaact
    51781 aattggatct ctgtgagata gtatcaaatg gctaatccca aagatgatag cagataggat
    51841 aacatccaaa tagtacttgg actagggaaa gaaggtattc ataaaatacc ctctatcaag
    51901 agtctcctca aaaacaggac cgatgattac aggcaggaca aaagataaga tagtcgataa
    51961 aaaggttggt tgtccatttg aaaaaagcac ggtaaaatac tcatcatgaa tattcctatg
    52021 attaatcaaa tgagcatagc gtgcccaaaa attaccgaga atctgataaa ccacataagt
    52081 tgcaaataag tagaagacaa atgaccagtt ccagctcttt ttctcaaaga taaagagcat
    52141 ctttttcttt tttaacctcc aaattaatag aaggaaactt cccactaatc ccattgttaa
    52201 aataagagaa tagacatcag ctcctaaccc taaaatgatc gtcacataca atccaattgt
    52261 ttgtggtaaa taggtagata gtaaaataat aagcaaaaat attccaaatt gtcttagttt
    52321 ttttgtgttt ctcatcgtac ttttttgaaa gattaccctg ctcggaagcc gtacttccaa
    52381 gcatctatat aagaattaag tgccccttgc ctcatatagg gagcaaattc tctataatat
    52441 aaccatctac tatatccatc ttcccaaaca gcaagaccac ctgaagtttg ctccaagtcc
    52501 tcagttgaaa gaactgtaaa tgtatttgta cctgtcattg caagtacctt cttaaaatag
    52561 attgttgtag gctcacattt atagtatatt tctttttttg tctattttat agcccatctc
    52621 ctcaactggc aatttttcga cctgaattac atttttccat aaaaaatgag acctttctag
    52681 tctcatttag tcattcttag tattttctaa atcgttgata gcgttcttcc agcaactctt
    52741 ctagcggttt ttgtgaaagt ctagccagct ccgtttggag ttcttttttg acactcttaa
    52801 tcagttcttt actagaaagt cctatttcag aaatcacctt atccaccacg tccatttcta
    52861 acagttcatg cgaagtgatt ttcatcagtt ctgctgcttc catagcgcga gtaccgtcct
    52921 tccataaaat ggaagcaaag ccttctggac tgagaatggc atagatagaa ttttccagca
    52981 tccagacacg gtccgcgaca gctagagcca gagccccgcc tgaaccacct tcaccgataa
    53041 taatggcgat aataggaact ttcaggtcac tcatttccat gagattgcga gcgatagctt
    53101 ccccttgacc acgttcttcc gctccgacac caggataagc acctgctgta ttgataaagg
    53161 tcacaactgg acggccaaat ttctcagcct gtttcatcaa ccgcagtgcc tttcggtagc
    53221 cttctggatg tggttggcca aaattccgtt tgaggttgtc ttgcaaactc ttgccttttt
    53281 ggataccaac cactgttaca gcttggtctc caagccaacc aataccacca acaactgcac
    53341 catcatcacg aaaagaacgg tcaccatgta attggataaa ttcatcaaaa atgcctgtcg
    53401 caaagtccaa ggttgtcaag cgactctgct cacgcgcttc tctgactatt tttgcaatat
    53461 tcatctagga ctccctccat gcaatctgac taggctagca atcgtatctg gtaagtctct
    53521 tcttttgaca atagcatcca caaagccatg ttctaatagg aattctgcct tttggaaatc
    53581 ctcaggcaag ctttcacgaa ccgtattttc aatcacacga cgcccagcaa aaccaaccaa
    53641 gctctgtggt tcagccagaa tgatatcgcc ttccatagcg aaagaagctg tcacaccacc
    53701 agtcgttgga tctgtcaaaa tggtcaggta aaagagacca gcatttgaat gacgtttaac
    53761 cgccgcagag atcttagcca tctgcatgag actcatgatt ccttcctgca tacgggctcc
    53821 accagaggct gtgaatagga caactggcaa tttttcgaca gtcgcatact caaacaaacg
    53881 agtgattttt tcacctacaa ccgtacccat agaagccatg ataaagttag aatccataat
    53941 cccaagagcc acagtctgac ctttaataag agcagttcct gtcacaacgg cttcatgcag
    54001 acctgttttt tcacgcatag atgccagttt cttttggtaa ccagggaaat gcaagggatc
    54061 cttgctttca atccctgtaa acaattcttt gaaggttccc atatcaatcg tcaaagccaa
    54121 gcgttcttgg gcagaaatac gaaaggtata gctacagtgc ggacagatac gttcacttcc
    54181 cagatccttc tgatagatgg tatgcttaca gcctggacac tgggaaaata attcatctgg
    54241 aacctctggc ttagcttgag gtttttccct aactgaacga ttgggattga ttcgaatata
    54301 cttatctttt ttactaaata gagccattga ttcccctttt cggtttaaac tcttaaagtc
    54361 attttattct ttttcttgat atttaggtaa gaaggtttcc atcaagaagg aagtatcata
    54421 atccccagca atgacattgc gatctgaaat gaggtcaagc tggaaatctg cattggtctg
    54481 cactccttca atctctaatt catagagggc acgttgcatt ttcatcaagg cgtcaaaacg
    54541 attttcgccg tgtactatga ttttggcaat catactatca taataaggcg gaatggtata
    54601 acctggataa actgctgaat ccacgcgcaa gccaactcca ccacttggca gatagagatt
    54661 agtaatctta cctggacttg gagcaaagtt aaaggctggg ttttctgcat tgatacgaca
    54721 ctcgatggca tgaccgcgta ggacaatatc ttcttgctta acagacaaag gctgacctgc
    54781 cgcaatgcaa atctgttcct taacgatatc aacacctgaa acaaactctg ttactggatg
    54841 ttctacctga acacgagtat tcatctccat gaaatagaaa ttgctacttg cttcatcaag
    54901 aagaaattca atggttcctg cattctcata gccaacaaac tctgccgctc gaacagcagc
    54961 agcacctatt tcatgacgca gcgtttttcc gattgcaatc gagggacttt cttccaaaac
    55021 cttttggtta ttcctttgaa gagaacaatc ccgttcaccc aagtgaatca catgtccatg
    55081 ctcatcacct aggatttgaa cctcaatgtg ccgagctgga tagataaccc gttctatgta
    55141 catggcacca ttgccataat tggccttggc ctcactagag gcagtttcaa aggcagaaac
    55201 gaggtcatct ggtttttcaa ccttacgaat ccctttacca cctccacctg ctgaagcctt
    55261 gagcataaca ggatagccaa ttttttcagc aacaatcaaa gcttcttcag agttatgcac
    55321 ttctccatct gaacctggta taacaggcac acctgcttta atcatctgag cacgcgcatt
    55381 gatcttatcc cccatcatat ccataacatg accagatgga ccgataaact tgatacctac
    55441 ttcttcacac atggtcgcaa atttggaatt ttcactgaga aatccaaaac cagggtgaat
    55501 agcttctgcc tcagtcaaga ctgcagctga tagaactgca ttaatattga gataagactc
    55561 tgttgccttg ccaggaccaa tacaaactgc ttcatctgcc aaaagcgtat gaagagcttc
    55621 cttatcagca gttgaataaa ccgctaccgt cgcaatcccc aattcacgtg ccgcacggat
    55681 aatacgaacc gcaatttcac cacgattggc aattaaaatt tttcgaaaca tggagaacct
    55741 ccttagttcc caattgcaaa agtaagggta ccactggctg caagcttgcc atccacttca
    55801 gcctttgctt caaccacagc tatggtgcca cgacgtttta caaaagtcgc tgtcataacc
    55861 aattggtcgc ctggtacaac ttgcttcttg aacttaacct tgtccatacc agcgtaaaag
    55921 accagttttc ctttattttc aggttttgat aactccaaca caccggcagt ttgcgccaag
    55981 gcttccataa tcagaacacc tggcataact gggtattgag gaaagtggcc gttaaagaaa
    56041 ggctcgttga tggtcacatt tttgatagca acaatggtat cctcgctcac ttccaagaca
    56101 cggtccacta gaagcatagg ataacggtgg ggaagagctt ctttgattcc ttgaatatcg
    56161 atcatttgat acgtaccaat cctttaccaa actcaaccat ttcttcgtta gagacgagaa
    56221 tttccgttac cacaccatcc ttaggagctg ggatttcatt catgactttc atggcttcga
    56281 taattaccaa tgtttgacct tttttgacac tatcaccaac tgtaacgaag gcaggtttat
    56341 ctggtccagc agccaagtaa accactccaa caagtggact ctctacaaga tttccctcag
    56401 tagccacact tgcttcagct ggagctggaa cttcttctgc tacagtctct gctggagcag
    56461 atgtaggagc tactggactc ggtgttgcta gaacgggtgc tggagcgact tgagttgcaa
    56521 cttcaggcac aggtctcgct tcattcttgc taaactgcaa ctcatccgtc ccatttttat
    56581 aagaaaattc tctcaaactt gactggtcaa attgagtcat caagtcttta atatcgttta
    56641 aattcatact tatctattct cccaacgttt gaaagcaaga actgcattgt ggcctccaaa
    56701 accaaaagta tttgaaatag cgtatggaat ttctttctcc aagccttgtc cataaacgac
    56761 attagcttcg atataatctg atacttcact tgtcccagct gtcattggta caaagttatg
    56821 acgcatagct tcgatggtga cgatagcttc tactgcaccc gcagccccca gcaaatgtcc
    56881 tgtaaaagac ttggttgatg atacaggtac ttccttacca agaacagcta cgatagcacc
    56941 actttctcct ttttcattgg caggagttga cgttccgtga gcattgacat aggctacttg
    57001 ctctggagaa atctcagctt cttccaaggc tagtttgatg gccttgatag ctccctgacc
    57061 ttctggatgt ggagaagtca tgtggtaggc atcacaagta tttccgtaac caaccacttc
    57121 agccaggata gtagctccac gtttttcagc gtgttcaaga ctttctagaa ccaacatccc
    57181 tgaaccttca cccataacaa acccattgcg atccttatca aatgggatcg aagcacgagt
    57241 tggatcctct gtagtagaga gagctgttaa ggcttggaaa ccagcgatgg caaaaggtgt
    57301 gatagaagct tctgttcctc ccaccaacat cacatcttgg aaaccaaact taatagagcg
    57361 gaaggcatcc ccaatcgcat catttgatga agagcaggca gtattgatag atttacaaac
    57421 accatttgca ccaaaacgca tggctacatt cccagaagcc atatttggta aagcttttgg
    57481 aagagtcatt ggtttgacac gtttgggtcc tttttcatga aggcgaagta cctgatcttc
    57541 aatttccttg attccaccaa taccagatgc aacgataaca ccaaaacgat ccctattaag
    57601 agcctctaca tcaagattgg catgatttac agcctcttgg gctgcataca aggcatataa
    57661 agaatagtta tcaaaacggt tggtatcttt ttttacaaag tatttatcga acggaaaatc
    57721 ttggatttct gccgcattat gcacatcaaa gtcactatga tcaaattttg taatgccacc
    57781 aatgccgatt ttcccagttg ctaaactatt ccaaaattct tctggtgtat ttccgattgg
    57841 agatgttact ccataacctg ttaccactac tcgatttagt ttcattcttt tcacctctag
    57901 ctttcgctac atacttaagc caccatcaat ggcaaccact tgaccagtta gataatcttg
    57961 gcctgctaaa aatactgtca aatctgcaac ctgctctgcc tgcccaaatt ctttcatcgg
    58021 aatctgagct agtgtagctt ccttaatctt atctgataag atagctgtca tatcagactc
    58081 aatcattcct ggagcaatca cattgactcg tatattccga ctagcgacct cgcgtgccac
    58141 agacttggta aagccaatca agccagcctt agaagcagca tagttagctt gaccaatatt
    58201 ccccatcaaa ccaacaacac tagacatatt aatgatagca ccttctctgg ctttcatcat
    58261 cggtttcaag actgattgtg tcatattaaa ggcaccagtc agattgacct tgagcacttt
    58321 ttcaaaatct gcttctgtca tcttgagcat aagagtatct tgggtaatcc ctgcattgtt
    58381 gaccaaaaca tctactgaac ccagttctgc aatagcttga tcaatcatac gcttagcgtc
    58441 tgcaaaatct gatacatctc ctgaaatggg aaccaccttg ataccatagt ttgaaaactc
    58501 agcgagcaat tcttctgaga ttgccccacg actgtttaag acaatgttgg ctcctgcttg
    58561 agcaaacttg tgggcgatgg caagaccaat tccacgactc gaacctgtaa taaagatatt
    58621 tttatgttct agtttcattt ttttcctttc aaaacttcta cttattttag tctatttttc
    58681 taaaagtgct actaaactcg cttgatcttc cacatgagct aagtgagcag tttgatcaat
    58741 ttttttaaca aaacctgaca agactttccc cggtccaatc tcgataaagt tgcttatgcc
    58801 tgcttcttgc atgaccccaa tactttcata gaaacgaacg ggttccttga cctgacgcgt
    58861 caagagctga gcaatgtcct ctttttgcat cacagcagct tctgtattgc cgactagggg
    58921 acaagtaaaa tctgaaaaac ttacctgagc tagagtttca gctagtttct ggctagcagg
    58981 ctcaaggaga gcggtgtgaa agggacctga caccttaaga ggaatcaagc gtttggcacc
    59041 tgcttcttgc aaaagttcaa ccgctcgatc aactgcaacc acttctccag caatgacgat
    59101 ttgtgcaggt gtgttatagt tggctggagt aaccactcca agttcagaag ctttttgaca
    59161 ggcttcttca atgacctcta ctggcgtatt gagaactgct accatcttgc cagagtcagc
    59221 aggagccgct tcttccatat aggctccacg cttagctacc aaggcaaccg catcttcaaa
    59281 atccaaggcg ccgcttgcca ccaaggcaga gtattctcca agagacaaac cagcaaccat
    59341 atcaggctga tagccctttt cttgcaataa acggtagata gcaaccgaag tcgctagaat
    59401 ggctggttgc gtatagcggg tctgattgag tttgtcttct tccgtatcga tgagataacg
    59461 taaatcataa ccgagcacct gactcgctcg atcaatcgtt tctttgacaa tcggatactg
    59521 atcatagaaa tcccgtccca tccctagata ctgggcacct tgaccagcaa ataaaaaggc
    59581 tgttttagtc atttcttaca actcctgccc agcgagaggc ttcttcttga attttcttag
    59641 cggctccgta atacaaatct tttaggattt cttcagctgt ttcttcttta gaaacaagcc
    59701 ctgcgatttg acctgccatg acagagccac catccacatc accgtgaaca actgctttgg
    59761 ctagggcacc tgctcccatt tgttcaaaga tttctaaatc aggatcttcc tgcttaaagg
    59821 catctttttc agccagttca aaatctctag tcaactgatt tttaatagca cgaacagcat
    59881 gaccaaagtg ctgagctgaa atcgtagtat caatatccct tgcttttaaa attttctcct
    59941 tgtagtttgg atgggcattc gactcttttg caactacaaa ccgtgtcccc acctgtacag
    60001 cctctgcacc tagcataaag ccagccgcag caccttcacc atccgcaatt cctcctgcag
    60061 caataacagg aatagatata gctgtggcta cctgtcgcac caaggtcatg gttgttaatt
    60121 taccgatatg ccccccagct tccattcctt ctgcaataac agcgtctgca ccgatttttt
    60181 ccatgcgttt agctaaagcg acactaggaa caacaggaat aacgattatc ccagcttcat
    60241 ggaaacgttc catatacttg cttggatttc ctgctcctgt tgtgacaact ttaacacctt
    60301 cttcaataac gagatccacg atatcttcca caaagggaga taagagcatg atgttgaccc
    60361 caaagggttt atcagtcaat gatttgattt tatcaatatt ggccttgaca acttctttcg
    60421 gggcatttcc cccaccgata attcctaatc ctccagcctt ggaaacagcc cctgccaaat
    60481 caccatcagc aacccaggcc atccctcctt ggaaaatagg atagtcaatc ttcaataatt
    60541 ctgtaatacg cgttttcata gtgcctccaa ccttccttgc ttacgtaata gttcgatttc
    60601 accataattt gacagtcaaa ctattaccta aacaagaggg agtgggtttc tccctactcc
    60661 ttctactaat attctgctta ttttgcttgc tcttcaacgt aagcaaccaa gtcaccaact
    60721 gttttcaagt cattttctgc ttcgatttgg atatcaaaag catcttcgat ttctgagatt
    60781 acttggaaca agtccaatga atctgcgtcc aaatcatcaa aagttgattc aagtgttact
    60841 tctgatgcgt cttttccaag ttcttcaacg ataatttctt gtactttttc aaatactgcc
    60901 atgataggac tcctttaaaa taaatagttt ttttataaca atgtgttcac cacatgatta
    60961 cctaaattgt aagaatgagc gtgccccagg tcaagcctcc accgaagcct gatagaagaa
    61021 cagtctggct accatctaaa gggatgagac cttgttctac acactctgaa agtaaaatcg
    61081 ggatactggc tgcactggta ttgccatatt ccatcatatt ggctggaagt ttggctcggt
    61141 caacaccaat ttttctagcc atcttatcca aaatacggtc attggcttga tgaagtagca
    61201 gataatccaa gtctgtcacc tctataggag attcatcaat agtctgcttg atagacttgg
    61261 ctacatctcg aatggcaaaa tcaaagactg tgcgtccatc catcttcaaa aacgaatctg
    61321 cactttcttg atctgaaaat ggagaatgca aacctgaatg cccataagtt aaacactcgc
    61381 tgcgacttcc atcgctattg agactctcag ctaagaaatg ctcttgctcg ctagcttcta
    61441 acaagacacc accagcacca tctccaaaca acacagctgt tgatcgatcc gaccaatcga
    61501 ctgccttaga gagggtttca ctaccaatca ccaagccttt ttgaaagcga ccagaagcga
    61561 taaacttttc agcagttgaa agagcaaata caaatccact gcaagccgcg gttaagtcaa
    61621 aagcaaaggc tttattagcg ccaatattag cttgaacacg agcagctgta gagggcatca
    61681 tcgaatctgg agtaatggta gctaggatga taaaatccag ttcttttcct gttattccag
    61741 cttttgccat cagtttctta gcaacctctg tagccaaatc actggtagat tctgttcttg
    61801 aaatatgcct ttgtcgtatt cccgttcgac ttgaaatcca ctcatcattg gtatccataa
    61861 tctgagccaa gtcgtgattt gtaaccactt gctctggcac ataatgagca acctgactta
    61921 tttttgcaaa agccattatt tcaaatcctc caaaaattgg taaagattag tcaaaccttt
    61981 acccatgaca gcaatttctt cctcgctcat gccatcaata attttttcta ccatggcctt
    62041 gtggaagcgt ttatgcagcc tatgaatcaa gcgacccttc tttgtcaaat gcagatgcac
    62101 cacacgacga tcctgttctg accgaactcg ctcaatgtag cccttacgct ctaaattgtt
    62161 caaacttgtc gtaacagttc caagagttac catcaactct tttgacactt gacttggagt
    62221 cacgtctgga gccttaccga tgacatcaat cgtatgcatt tctttgatgg agatatcctt
    62281 aaaacgacta cctctcaagt tcacttcctc aattacaagg acattgttaa atatagatgt
    62341 taaatattca ttgattcgtt ggtagtccat ttttcatatc cctccttctt caaaaaactt
    62401 ttacaatcaa aacatttgac agaaagaatt atatcaaaat tcaaagaatg atgcaagtat
    62461 tttttatttt cctgtaaatt taggtcttct tctctccgaa tgagctcgca ctccttcttt
    62521 gaaatcctct gtttgagcta aggatttctg taggttcagt tctaaagtag cgtaaccctg
    62581 ccagtcttta aattggctct cccaaaccaa cttcttaatg gcagcatagg aattacttga
    62641 agcacgtctt aattttttaa gaagctgttc tctcgtcttt tcaagttttt cagcttcaga
    62701 aacgcgataa accaaccccc attctagggc tttttctgcc gttaaagcct ctcctgtcat
    62761 agctaattga gcagcacgcg tcacaccgat actgcggctc aagagatgaa ttccccctgc
    62821 atctggagcc aaaccaacac caacaaaggc ttggataaac ttagccttat ccgtcgccaa
    62881 acagaaatct gccgcaacag ccatattcgc tgcggcacct gcaacagcac cgtcaacttc
    62941 cattaaaaca ggtttggcta tttgcttgat tttataagaa atcgtattga ccaactctgc
    63001 gatttttgtc aatgatggaa tatcatcctc atccactgcc cgcttcatct ctaccaaatc
    63061 tcccccaact gagaagacct ttccattggc attgattaag ataaaatgca cagctggatt
    63121 ttcttctgcc aaagtcagag cttctaaaat ctcctcacac atgggaatat gaaaaccatt
    63181 tgcgacctca ggacggttca aggtaaggat tgccaagtcc tcttcaagct gataaataat
    63241 gtgttccatc agggctcctt tttattttat aagcagttta tactcttcga aaatctcttc
    63301 aaaccacgtc agcttccatc tgcaacctca aagcagtgct ttgagctgac ttcggctagc
    63361 ttcctagttt gctctttgat tttcattgag tgttaaatta tttcactgtc aaagtatatc
    63421 ttttttagga gcagaaagca agaaaaaatc gactgaaagt gtctcagccg ataagaaagg
    63481 ctcgctttag gctagaaagc atcttatttt cttcattgaa aaaagccttt ctttctgcta
    63541 taatcgtata aaatacttac tttaggagtt cttatgaaag ttgttaaatt tggaggtagt
    63601 tctcttgcct ctgctagtca attagaaaaa gttttaaaca tcgtcaaaag cgattcagag
    63661 cgtcgttttg tagtcgtttc tgcgcctggt aaacgcaatg ctgaagatac taaggttacg
    63721 gatgccctga ttaaatacta ccgcgactat gttgcgggta acgatattag caagagccaa
    63781 agctggatta tcgaccgcta tgctgctatg gttagtgaat tgggactaaa accagctgtg
    63841 ctagaaaaaa tttctaaaag cattcacgcc ttggccactc ttcctattga agacaatgaa
    63901 tttctctacg atactttcct agcagccggt gaaaataaca atgccaaatt gattgctgcc
    63961 tactttaacc aaaatggtat cgatgcacgc tatatgcacc ctagagaagc tgggattgtg
    64021 gtcacaagtg aacctggtca cgctcgcatc attccatcaa gttatgacaa gattgaagaa
    64081 ttgacaaaca ccaatgaagt ccttgtcatt cctggtttct ttggtgtcac taaggaaaat
    64141 caaatctgta ctttctcacg tggaggttct gatattacag gttctatcat tgctgctggt
    64201 gtcaaagccg acctctatga aaactttacg gacgttgatg gtatctttgc agcccaccct
    64261 ggtattatcc accaaccaca ctcgattcct gagttgacct accgtgaaat gcgcgagttg
    64321 gcctatgcag gcttctcagt ccttcatgac gaggctcttc ttcctgccta ccgtggaaaa
    64381 attcctctgg ttatcaagaa taccaacaac cctgaccatc caggtactcg tatcgttcta
    64441 aaacacagta atgatgaatt tccagttgtg ggaattgctg gtgactcagg ctttgtcagc
    64501 attaatatgt cgaaatacct catgaaccgt gagattggat ttggccgcaa ggttctgcaa
    64561 atcctggaag aacttaacat cggttgggaa catatgccaa caggtatcga cgatctttct
    64621 atcattctcc gttctcgcca actaactccc atcaaggaag aagaaatcct gcgtcagttg
    64681 gttcaaaagg ctaaagtaga tcatgcagaa atcgaacacg acctttctat cattatgatt
    64741 gttggtgaaa aaatgaagag ccacatcgga gtgactgcta ctgcgacacg cgctctatct
    64801 gaaaacaaaa tcaacatcca gatgatgtct caaggttcta gtgaagtttc tattatgttt
    64861 gttgtcaata aagaccaaga gaaagcagca attaaagccc tctacaatgc cttttttgga
    64921 gaaagtaagg aagactaagc atggcccaat cacttaacaa aacagtactc ctcagtacga
    64981 caggcacttc ctacctctct atcgctggga aagttgggaa attccttgtc ggagatcagg
    65041 ctttggaatt ttacccagat gtcaatgtcg aacaatttat ccagattcct tggagccata
    65101 tcaatcagat tggagccaat gtcactggac gcaaaatcag ccgccacttc gaagtcttta
    65161 cagacaaagg aaaattcctc tttgcttcaa aagactcagg tgccattctc aaaattgcac
    65221 gcgaaaaact aggcaatgac aaggtcgtca aacttccgac cctgattcag acaattagcc
    65281 aaaaatttaa aaatctattt gcaaaaaagt agaaacttta gtataatcat agcaacggat
    65341 aagtaggtta tcttcttctt tcagaaagtc tgcgggtact gcgagcagat aggagggata
    65401 ggtgaaattc taccgttagt aacaattaca tacacaatca agtgcacacc tgtgaagttg
    65461 gatggaaccg tggccctgcc actccaacgt tttgtcaggc gtgctttttt cataaaggag
    65521 ttcttatgtt agatatcaaa cgtattcgta cagattttga agctgtcgca gaaaaattag
    65581 ctacacgtgg tgtagatgct gctgtcttga atgaaatgaa agaaatcgat gctaaacgtc
    65641 gtaacatctt ggtcaaggtt gaaactctca aagcagaacg taacacagtt tctgctgaga
    65701 ttgcccaagc taagcgcaac aaggaaaata cagatgacaa gattgctgcc atgcaaaatc
    65761 tatctgctga ggttaaagcc ttggatgctg aattggcaga aatcgatgct aaattgacag
    65821 aatttacaac gactcttcca aatatcccag ctgacagcgt tcctgttggg gctgacgaag
    65881 acgacaatgt ggaagttcgc cgttggggta ctccacgcga gtttgacttc gaacctaaag
    65941 ctcactggga tctcggtgaa gaccttggta ttcttgactg ggaacgcggt ggtaaggtaa
    66001 caggcgctcg cttcctcttc tataaaggcc tcggtgctcg tttggaacgt gctatctaca
    66061 actttatgtt ggatgaacat ggaaaagaag gctatactga agtcatcaca ccttacatag
    66121 tcaaccatga ttctatgttt ggtactggtc agtatccaaa atttaaggaa gatacttttg
    66181 aactcagcga taccaacttt gtcttgattc caactgctga agttcctctg acaaactact
    66241 accgtgatga aatcttagac ggcaaagatc ttccaatcta cttcactgcc atgagtccgt
    66301 cattccgttc tgaggctggt tctgccggtc gtgatacgcg tggcttgatc cgtttgcacc
    66361 aattccacaa ggttgaaatg gtcaaatttg ccaaaccaga agaatcttac gaagaattgg
    66421 aaaaaatgac agccaacgct gaaaacattc ttcaaaaact caaccttcca taccgtgtcg
    66481 ttgctctctc tactggagat atgggcttct cagctgcgaa gacttacgac ttggaagtat
    66541 ggattccagc acaaaacaat taccgtgaaa tctcaagctg ttcaaacaca gaagatttcc
    66601 aagcccgtcg tgcccaaatc cgttaccgtg atgaagcaga tggcaaggtg aaactccttc
    66661 ataccttgaa cggttctgga cttgcagttg gacgtacagt ggctgcaatt cttgaaaatt
    66721 accaaaatga agatggttct gtgaccatcc cagaagcact tcgtccatac atgggtggag
    66781 ctgaagtcat caaaccataa aaaataaggt ttagctattt ctagctagac cttttttcgt
    66841 aaccaaatca gataagcacc tagtacaaag aataaaatag ttaggcatat aatggtttca
    66901 gccaatacca ggtaatccag aaatggaagt ttcaaaattc cctgagccat cttgagcgag
    66961 gtcgctgtga taatggttgg gaaggtgagg gctgagaagg ctggttgaaa accttgtttt
    67021 aaaatgttgg gcagacgagt taaaacaaag aaaaagaagg attgagaagc caaaatcatg
    67081 acaatcaaga cccaagtcgg caggctggtt cctcctactc gaactagaga agccaagagt
    67141 agagagaaag gagcacagta gattccttct tgtccaagca aggctagtgg gagtggatgt
    67201 ttctttaaat cgctataaat aaggggatag agatagaagg tcaagagaaa accaaaactc
    67261 aaggtcgcat aggccatttc gataatacct accagaggat aggtcaaggc agccactgct
    67321 atccccacat agagaaccgt ccaacttgga gtggcatgaa ccctccgccc tggacaagca
    67381 aacttgatgg taaaaccagc aatcaaggtc aaatccaaga gaaatgaaaa ccaccaaatc
    67441 ccttgtgcta ccaaaggaag ataagagaat acgcgaaaga cataggtcga taaaatcatc
    67501 ccagccatag gaaaggttgc cattcctgac aaaagagggg gcttggtcaa ttcttgcttg
    67561 gtttctttcc aattaaagag atgcagaatt agaaagtaaa tccataaaac caaaccagtc
    67621 agactaaaaa gatgggatag aactggcaac gtatctaaaa taagatttcc agctcctgcc
    67681 aaacctagca aacaacctga aaatactaag gggagttttt tcatcctaac ctccaataat
    67741 catgttagtt tcagtataac ataaaagcgc ttaaatgagg atttaaaaaa acgaagtccg
    67801 attatttcag acttcatttt actcagatat gaattaggca taaggttgca attctggatt
    67861 aattggtgta ttagctaagt tgttggcata gttacagagg attgctaggc tgacaccaaa
    67921 aaccacatcc aaggcatttt gttgagtgta gccagcttct aaaaactcag acaaggcttc
    67981 atctcctaca cgacccttgg tattgataac tgccaaggta aacttagcta gggtatccaa
    68041 tttaggatct gtttcaattg gagtacgatt gcgaagagct tgaatcaagt catcattcat
    68101 ctggatttgt ttgatggaaa aggctgtgtg acctgcgaca cagaaggcac aaccattggt
    68161 cacggctgcc gtgatttgca ccacttcacg ctcaacgggt gtcaggctgt tgcgacggtg
    68221 gatagatgag acaatttggt aggcttctaa aacagtcggg gcattggcca agagaccgat
    68281 taggttggga atatagccat tgttgtcttt ttctactgtt tcaagaattt ctttcacttc
    68341 tgctggtgct gactctactg tatggatagt aaatgttgtc ataagatacc tcttttctta
    68401 ttattgacac taatattatt ggaaaatctt ataaaatcct gattcctaag tttatctaag
    68461 ataaagcttt attctctcat aagattttcg ttgttatatt agtttatcac acttccaatc
    68521 acttgcataa tatatattat atatcaggct gataaaaatt atttataggc aaaaaaatca
    68581 cacgagctgt gtgattccat tatttgtcaa aatacttttt agtttcagca ataacgactg
    68641 gcgacaagac caagagggca atcaagtttg gcagagccat caaggcgtta acgatatctg
    68701 cgataatcca gaccatatcc aactcgataa atcctcctaa caagaccatg agcacaaaaa
    68761 ccacacggta gagccagata aagcgaaccc caaagaggaa ctcaaaacag cgttctccgt
    68821 aatagttcca acctagaatc gttgtaaagg caaaaagtac aaggaagatg gtcaagagag
    68881 caggcccaaa gtgtgaaaag actgttgaga aagctgactg agtcaaggca accccattca
    68941 agtcaccgct ccaaactcca gttaccaaga tggtcaaacc agttagagta caaatgatga
    69001 gggtatcaat aaaggttcct gtcatggaaa tcaaaccttg ctctactggt tcatttgtct
    69061 tggcagctgc agctgcaata ggagcagaac ccagaccaga ttcgtttgag aacacaccac
    69121 gcgccacacc attttgaata gccatccgaa cgctagcacc agcaaatcca cctaccgcag
    69181 caaggggact aaaagctgag gtaaagacta aagcgattgt gccagggatt tttccgatat
    69241 taaagaaaat aactgtaaga gttcctaaga tataaatgag ggccataaaa ggaacaacag
    69301 tagttgaaac cttagaaata gacttgagtc caccaaagac tgcaatcgct acaaagacag
    69361 acaagacgag agatgtgatg gctggcgaaa tcgtcgttgt attttggata gattctgtaa
    69421 tcgagttgac ttgggtgaag gttccgattc ccaagagagc aaccaatact cctgctactg
    69481 caaacaaaac agcaagtggt cgccactttt ctcccatccc tagaaggata tagtgcatgg
    69541 gacctcccgc tactgcacca tggtcgtcct tggtgcggta tttgatggcc aagagtcctt
    69601 ccgcatactt ggtagccatt ccaaagaaag ccgccatcca catccaaaat agagctcctg
    69661 gtccaccaac cttgatagcc gtcgcaactc ctatgatatt tcctgttcca acagttgatg
    69721 ccaaggctgt acacagagct gcaaaactgg atacatcacc atgtccctta tcctggataa
    69781 aaataagctg aaaggccttg ggtagacgca aaacctgcaa gagtcctagc cgcatggtta
    69841 ggtaaatccc tgttccgacc aataaaatca agaggggcgg tccccaagca aaagcatcga
    69901 ttgatttaag caattctaac atttccttct cctatcgttt caaccccaaa agaaagagca
    69961 catgcaagat acatgtactc tggaatgctt agataaatgc taaaaagcgg tctatcctag
    70021 ctctgtcctt ttacctgaga gtttgagcag ttgcctgcct tgccccttcg gtgcctttac
    70081 ggtctctcca gagttccgtc catttacagt catggaaaat caaacgattc cccacttcta
    70141 ttaaacttca ttcggtgttg gtatttaatt gattctaatt ttacaaaaaa tgttggcttt
    70201 tgtcaatgtg tttattagta aaaattagtt caacagtttt tactttataa agtccagaat
    70261 actgctatcc tttaaaagtg acaatagtcg caccactgcc tccagcattt tgtggggcat
    70321 agccgaaact cttgacatgt ttgtttcttt gcaagtattt ggtaactcct tcacggatga
    70381 ctcctgttcc gataccatgg atgatatcaa cttgagccat attgttaagc aaggcttggt
    70441 cgatgaaggt atctagctca ttcatggctt cttcatagcg cttgcctcga agatccagtc
    70501 tagcttgagg tcctcgccca gaagttcgtt tcacaacatt gacctgtttc ttcttgactg
    70561 gtttttcttg ctgggcttga acaagatcaa actctttctc ttccaaggtc atcttaatca
    70621 agccaacttg ggcttcccag cggccgtcct tgagttgact ggtcaaggta ccacgctgac
    70681 cataactgag aaccacgata tcatctccca cctttggagc tcgttttttc ttggcctttt
    70741 gaaggacctt gtttttagac aagtccactt tttcaggagc caattttttc aacttggcct
    70801 tggcttcaat gatttcgtgg ggcttgagtt gggatttact gtggagattt ttgagaatct
    70861 ggtcactttc acttagggcc atatccacaa tctcagcagc ctgttcacgc gccttgttaa
    70921 gctcggtttc cttttcacga ttaagctcgt tgtagagttt ttttagagca cggttcatct
    70981 tgagattttc ttgctccacc tcacggatat tgtccaagcg tttgcggctt tccagcgtct
    71041 gctcttctaa ttgctcaatg atacgattga cgtcattgtc ctgatcaatc tgctgactgg
    71101 catctcctac gataacttca gataggccta gacgtttggc aatttcaaag gcattacttc
    71161 ggccaggaac accctgcata aagcgatagg tcgggcgaag agttgcagta tcaaactcca
    71221 tactggcatt ttgcacaaag gctgtctcaa taccgtaggc cttgagttct ggatagtggg
    71281 tcgtcgccat ggtcttgatt tgacgcaggc gaaggtcctc cagaatagcc atggcaaggg
    71341 ctgctccctc ttggggatca gtaccagccc ccaactcatc caaaagtaag agtgaatgtt
    71401 ggttgacctt gccaagaata tccacgatat tggtcatatg gctagagaag gtagacaagc
    71461 tctgctcaat agactgctca tctccaatat cagcaaagat ttcttcaaaa ataccaacac
    71521 gacttccctt gtctgctaaa atcggcaatc ctgactgggc catgacctgt gtcaagccca
    71581 gagttttgag catgatggtc ttcccacctg tattgggacc tgtaatgaca atagctgtta
    71641 aatcttgacc aaaatagaca tcatttgcga cggcattttt gaccaaagga tggcagacat
    71701 ggagcagttg aatctcttga ttttctgaca gctgaggcac gactgcttgt ctttcttgga
    71761 taaatcgaac cttggcacga atcaagtcca gatgaccgat aatccaagcg tcattagcaa
    71821 tctcagccgc atgagggcgg acacgctcag aaatttcttg gagaatgcga agcatttcat
    71881 agcgctcatc tgctcgcaga ctagcaattt cttcgctcag tttgactacc tcacgtggtt
    71941 cgatatagac ggtgtttcca ctagcagaaa tatcatgaac gacacctgca atcttattgc
    72001 ggtaggtgtt tttgactggt aaaacctgac ggccatttct gctagcaaca attccttctg
    72061 tcaacatctg cgctttttgc ttgagcaagt cttgtaaaac atcgcgtacc tgactctcgc
    72121 tatcatgtat ttttcgacgg attcgcgcca attcttcact ggcaaaattt tcaatgaaac
    72181 ccgcatcatt aaaggcctga agatttcctt gtaattgcgg aaaatcatgt aatttctcaa
    72241 accaaagggc taattcttcc aagctgacat tttccagatt ggcgtaaaaa ctttgaagtt
    72301 ctcggctggc aagaagcacg cgtttcaaga gtaggaactc ctcgatattg agatccgctc
    72361 ccatctccaa cctcttgcag actcctgcaa tttccttagt tgagagaata gtaaaatgcg
    72421 gttgctcgac gaaaagagcc tgcatttcct tcatctcagc aaaagcctgt ttgattttat
    72481 ctgctttggc agtcggagcc agttgtctca attgctccaa gccctgctcg gtcaacaaat
    72541 gaggctcaaa caaggccttg accttatcga actctaatgt ttctaatatt ttcttattca
    72601 tctttatttc cttgtctttg aatgtctagg tgtggtagct ttttacattc tgatagattc
    72661 gagtcaaatc tgtaaacaaa gggctaggaa aactcctgcc ctttttatcc gattaaattt
    72721 gtcacccaga tttgtttgag ccaactggtt gttatcggta tgctctggat gatgtgtttt
    72781 gcgacgatac tcttttcaag aggattttgt ataactgcca tggggatggt cgccaagatt
    72841 gtcaaggcca tttgcaagac aaataaggtc accaacatgg acaagatacc tgctgaaact
    72901 tggaacaact taccacccag ttttttgcta ggaatcaagt gtaagagaag accaagcaaa
    72961 cgaccaatgc tatagacaat cccaaataca agcaagtagc cgatgcctgc ataaaagacc
    73021 ttatccagct gaaagagttg atccgatggg aaaaagaaag tcccctgacc ttcctgcgaa
    73081 tttgcataag ggagcaataa atggaattgc tctccaagcc ccttataaaa ctggccagcc
    73141 ataaaagccg atgccatggc tgaaatcagg taataaacct gtaagagcag gcctctccga
    73201 tagccgatat aaaatcccca aaccaagacc aatagaagaa ggaatgaaat cataaggaat
    73261 cctcaatctt gctctgttct tgcttacaag tcacaagctt gtgacggagt tcttctagct
    73321 cttgctcctt atcgtcaaat tcaatctcac ggctgagctg agttgataaa cagttgactg
    73381 ccaacaaaag agcgattgtt tcatcatctg cgctaggcat ttgttcttta attgcttggt
    73441 atttttctgt cgcaacctta gcgatttcct ccataaaaag gttatcatgt tcgcttgtca
    73501 aggttaacga ttttttcccg aatgtaaatt tgaatcgatt tagatttgcc ataaaattca
    73561 cctcacgata ttataccaaa attcgctaat tttgtcagtt tttacaaatt ttactgcttt
    73621 tgtggtacaa tagaaactat ggcaagtatc acactcacac caagcgaaaa ggatattcag
    73681 gcttttcttg aacactatca aaccagtctg gctcctagca agaatcccta tatccgctac
    73741 tttttgaaac tacctcaagc aacggtttct atctatactt ctggaaaaat cttgcttcag
    73801 ggtgaagggg ctgaaaaata cgccagtttc tttggctatc aagctgtaga gcaaaccagc
    73861 ggacaaaatc ttcctttaat tgggacagat gaggtgggaa atggttccta ctttggtggg
    73921 cttgcagttg tggctgcctt tgtcacacct gaccagcacg actttttacg aaaactcggt
    73981 gtgggggatt ctaagactct gaccgaccaa aagatccgtc agattgctcc tattctcaag
    74041 gaaaaaattc agcaccaggt actccttctc tcacccagca agtacaacga ggtcatcgga
    74101 gaccgctaca atgctgtttc ggttaaggtt gccctccata atcaggctat ctatctcctc
    74161 cttcaaaaag gtgttcagcc tgagaaaatt gtgattgatg cctttaccag tgctaaaaat
    74221 tatgacaagt acttggcaca agaggccaat cgtttcagca atcctatcag cttagaagaa
    74281 aaggctgagg gcaaatactt ggctgtcgca gtttcttctg tcattgcgcg tgatctcttt
    74341 ctggaaaatc ttgaaaatct gggacgagaa ctgggttatc agcttccaag tggagctgga
    74401 acggcttctg acaaggtggc tagccagatt ttgcaagcct atggtatgca gggactcaat
    74461 ttctgcgcca aattgcactt taaaaacact gaaaaagcga aaaaacgctt agaaaggtaa
    74521 gttatgaatt catttaaaaa tttcttaaaa gagtggggac tgttcctcct aattctgtca
    74581 ttactagctt tgagccgtat ctttttttgg agcaatgttc gcgtagaagg acattccatg
    74641 gatccgaccc tagcggatgg cgaaattctc ttcgttgtaa aacaccttcc tattgaccgt
    74701 tttgatatcg tggtggccca tgaggaagat ggcaataagg acatcgtcaa gcgcgtgatt
    74761 ggaatgcctg gcgacaccat tcgttacgaa aatgataaac tctacatcaa tgacaaagaa
    74821 acggacgagc cttatctagc agactatatc aaacgcttca aggatgacaa actccaaagc
    74881 acttactcag gcaagggctt tgaaggaaat aaaggaactt tctttagaag tatcgctcaa
    74941 aaagcccaag ccttcacagt tgatgtcaac tacaacacca actttagctt tactgttcca
    75001 gaaggagaat accttctcct cggagatgac cgcttggttt cgagcgacag ccgccacgta
    75061 ggtaccttca aagcaaaaga tatcacaggg gaagctaaat tccgcttctg gccaatcacc
    75121 cgtatcggaa cattttaaga aacctaagag gccgagaatc accaatctca gcctcttctt
    75181 ctatcgtgag aaaatgattg gtaactatct aaacttacca gaacagaaac acctcaactc
    75241 tcacctattc atgcaaagga attttatgga agtttatttt tcaggaacca ttgaacggat
    75301 tatttttgaa aatcccagca atttttatcg catcctcctc ctagaaatcg acgatacgga
    75361 cgcagaggat tttgatgatt ttgaaatcat tgtcacagga accatggctg atgtaattga
    75421 gggcgaagac tatacttttt gggggcaaat tgtccagcac tccaagtatg gagaacaact
    75481 gcaaatcagt cgttatgatc gcgcaaaacc aactagtaag ggcttggtca agtacttttc
    75541 aagtagccat ttcaagggga ttggtctcaa gacagctcag aaaatcgtgg atacctatgg
    75601 cgaaaatacc attgacgaaa ttttgcaaca cccagaaaag ttagaaggca tcgcaggact
    75661 ctctgccaaa aatcgcgagg ctttcgtctc cactctccgt ctcaactacg gaacggagat
    75721 ggttttgtcc aaactagcca actacggcat tcccaacaaa ctagcctttc agattcaaga
    75781 cttttacaag gaagaaaccc ttgatgtggt tgaaaattat ccctaccagc tggttgagga
    75841 tatcaagggt ttgggcttta ccattgctga ccaactagct gaggaactag gcatcgaaag
    75901 tcaggctcct gaacgcttcc gcgccggtct agttcacagt ctttttcagg cctgtatgga
    75961 aacaggggac acctatgttg aagcacggga tttgctggaa caaaccctta ctctccttga
    76021 gtcttcccgt cccgtggaac tagaccccag ccaagttgcc caagagctct cctacctgat
    76081 cgaagaagac aaggttcagc agattgatac caagatcttt gacaacagcc tctttttcgc
    76141 tgaggaaggc atccgcagtc acttggttcg tatccttgaa aaaggaaaac agaaaagtca
    76201 tgatttaaaa accattcaaa aacatatcac tactatcgag aaagagcttg gaattgagta
    76261 tgataacatt caaaaacagg ctatctgtga tgctatccag aataaggtct ttatcctgac
    76321 aggtggacct ggtactggta agacaactgt tatcaatggg atcatcgctg tttacgccct
    76381 gttagaggga cttgacctca ggaagaaaag caacctgccc attcttcttg ctgctccaac
    76441 tggtcgagct gctcgtcgca tgaatgaatt gacaggtttg cctagcgcga ccatacaccg
    76501 ccacttggga atgacaggtg acgatgatac cagtcatctg gaagattacc tagatgctga
    76561 ctttatcatc gtggatgaat tctctatggt ggatacttgg ctggccaatc aactcttctc
    76621 caatatctct tctaacagta agatcctcat cgtaggtgac agtgatcagc taccttctgt
    76681 cagtcctgga caggttctag cggatctgct tcatattcct ttgattcctc agactcgctt
    76741 ggaaaaaatt tatcgacaaa gcgaagaatc aaccatcgtc accctagcta gtcagattcg
    76801 acagggcatc ttgccagctg atttcaccca aaaaaaagct gaccgttcct actttgaaat
    76861 tgctagtggc catattcctg ccaccattga aaaaatctta ggtgccgccc tcagaaatgg
    76921 tattcctgcc cgtgatatcc aagttctggc tcctatgtac cgagggacgg cagggattga
    76981 tgctatcaat cagctcatgc aagacctgct caatccccca caaaaagatc aactcagttt
    77041 tgaagctcct cagtgccact atcgtaaggg agacaaggtc attcatttgg ttaacgatgc
    77101 tgaaatcaat gtctttaatg gggatttagg agctatcaca gacctgattc ctggtaaata
    77161 caccgaatcg aaacaagacg agattgtcat tgattttgat ggtaatgaag tctcttaccc
    77221 ccgtaacgaa tggtacaaga ttcgcttggc ctatgccatg agtattcata agtctcaggg
    77281 aagtgagttc ccagttgtca tcctaccgat tactagtgct agccgacgta tgctggagcg
    77341 aaatctcatc tacacagcca ttacacgtgc caaaagcaaa ctcatcttac taggcgaatt
    77401 acaagccttc gactatgcta cccaacacat cggaactgcc cgaaaaacct atctgattga
    77461 acgcttcagt gatctactgg agaatgttga agaaaagcaa caagctgtct ctgaaacagt
    77521 cacatcaagt gcctctgaac aatcctacat cctaaccgaa gaaaactggg acagcattcc
    77581 agccatgatt gggattacag acacagacct caaagagatt tttggaaaat agtgcatcag
    77641 aaaaaccacc aattcaggtg ggttttctgt ttattaagat tattttactg ttgctgtgct
    77701 tgtaatcaat tcaacagctt ttttgatagt gatatcatgt ttcaacatgt cagctgaaag
    77761 caagttttga acttgtgcaa cttccatgtt gtagtctgct gccaattgct caacttcttt
    77821 ttggatttct tcttctgaag catcaaatcc ttcagctttg gcaactgctt cgataacaag
    77881 gttagtctta gtacgtgact cagcttctgc ttggtattgg ttgtgaaggt cttcttgagt
    77941 agttccagtg atttggaagt acatgtcagg gttgatccct tgacgttgca agttcccaag
    78001 gaattcattt actgaacggt gaacttcttc gtggatcatt tcttctggaa gttctacgat
    78061 ttcagcattt tctacagctg tatcaattgc tgcaccttca actgcatctt tgtaagcttc
    78121 ttctttagca gtagccaatt ctttgcggta tttttctttc aagtcagcaa gtgtttcaac
    78181 ttcttcatca atgtcttttg caagttcatc gtcaagagct ggaacttctt tagcttttac
    78241 ttcgtggata gttgtcacga atttagcttc tttacctgca aggtcttctg cttggtagtc
    78301 ttctgggaat gttacgataa catcaacggt ttcgccagct gagtgaccta ccaattggtc
    78361 ttcgaaacca gggatgaatt gacctgaacc aagtccaagt gagaagtttt caccttttcc
    78421 accgtcaaat tcaacaccgt cgatagaacc aacgaagtcg atcacaacag tgtcgccgtt
    78481 ttcagcagca gcttccttga taaccaattc agccagattg ttgcgttcgc gttcgatacg
    78541 ctcttcaaca tcagcgtcag ttacttcttt ttctacatca actgatactt caaggttttt
    78601 gtagtcaccc aattttactt caggttttgt aacgacttca gcagtgataa cccagtcttg
    78661 acctttttcc attgaagtta cgtcaatttt tggttgggca accacttcaa gaccagcttc
    78721 ttttacagct gcttcataag cgtttggcaa aagagcgttc ataacgtctt ggtaaagtga
    78781 ttcttcacca aattttttat cgaagatagg acgtggaagg tgacctttac ggaaacctgg
    78841 aacattaaga gatttcttca ctgacttgaa gacacggtcc aattctggtt tgatttggtc
    78901 ttgagagata gtgaaagtca agacaccacg gtttgtttct ttgttttcaa atgatacaga
    78961 cattctgtca tttctcctta aaatttttta aatacagtct attataacat tttctataaa
    79021 taaaatct
//
LOCUS       DAVKWZ010000002        76869 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000002 DAVKWZ010000000
VERSION     DAVKWZ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 76869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76869
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.002"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            101..2230
                     /locus_tag="VW854_000079"
     CDS             101..2230
                     /locus_tag="VW854_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEV0261393.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRAQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYTAVKELFKRL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVTVWVKKIDTEREKVNLSLLAPNETD"
     gene            2217..2666
                     /locus_tag="VW854_000080"
     CDS             2217..2666
                     /locus_tag="VW854_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEV0261394.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQKLGMEVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPLKLYRCQSCQQRYQRKRRIDTKRYRCGLCRGKLLLINQPED"
     gene            2725..2997
                     /locus_tag="VW854_000081"
     CDS             2725..2997
                     /locus_tag="VW854_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV0261395.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(3351..3542)
                     /locus_tag="VW854_000082"
     CDS             complement(3351..3542)
                     /locus_tag="VW854_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261396.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFIGTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            3969..4727
                     /locus_tag="VW854_000083"
     CDS             3969..4727
                     /locus_tag="VW854_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0261397.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFDEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            4729..6717
                     /locus_tag="VW854_000084"
     CDS             4729..6717
                     /locus_tag="VW854_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0261398.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTVTYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQTKVVITVLV
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSSESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYKIKEKEVFRYTYFAVANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNEGVKEQKALTLNDHQFSVKEEFTKDFIVNHVPNQF
                     NILTADYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVNASEAEQLKVAEEYEKYLQKF
                     NAQLNTEGNYVYGSTLADASAQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFLHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(6760..7404)
                     /locus_tag="VW854_000085"
     CDS             complement(6760..7404)
                     /locus_tag="VW854_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEV0261399.1"
                     /translation="MLRAGVLDANDGIISIAGVVIGVASATSNIWIIFLSGFAAILAG
                     AFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYASYIQNGEFKTSAQLL
                     TNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSDYR
                     IPATVLIVGVALLLTGYTSARLGKAPTKTAMIRNLAIGLLTMGVTFLLGQLFSI"
     gene            complement(7430..8305)
                     /locus_tag="VW854_000086"
     CDS             complement(7430..8305)
                     /locus_tag="VW854_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV0261400.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNRENASLLGKAPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVKKSSLTKELKEKILHYHNQ
                     KFSPDKKQASTNFKPAGQSIEQRSEAINLRLENGYYEIDTVLLTRAKNYCLLVLTDRK
                     SRHQIIRLIPNKSAEVVNQALKLILKQHKILSITADNGTEFNRLFDVFSEEHIYYAHP
                     YASWERGTNENHNRLIRRWLPKGTKKMTPKEVAFIEKWINNYPKKCLDYKSPREDFWM
                     ANLNLKFSVRNKSRN"
     gene            9012..10487
                     /locus_tag="VW854_000087"
     CDS             9012..10487
                     /locus_tag="VW854_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV0261401.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTDKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSILLIGEQMNSEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEEVIELSEYARREINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDESVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
     gene            complement(10453..11168)
                     /locus_tag="VW854_000088"
                     /pseudo
     CDS             complement(10453..11168)
                     /locus_tag="VW854_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            11255..11392
                     /locus_tag="VW854_000089"
     CDS             11255..11392
                     /locus_tag="VW854_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261402.1"
                     /translation="MRYQTHYQLKQKLWLEPFGNRQAIGGNELIHSGLFQVYHLGFGS
                     S"
     gene            11423..12283
                     /gene="speE"
                     /locus_tag="VW854_000090"
     CDS             11423..12283
                     /gene="speE"
                     /locus_tag="VW854_000090"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEV0261403.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQSFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVE
                     DILEEEEGKK"
     gene            12280..13539
                     /locus_tag="VW854_000091"
     CDS             12280..13539
                     /locus_tag="VW854_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEV0261404.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLLDPASLGPRTVGKTNIGCIFTGV
                     KDGVKKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            13539..14666
                     /gene="nspC"
                     /locus_tag="VW854_000092"
     CDS             13539..14666
                     /gene="nspC"
                     /locus_tag="VW854_000092"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEV0261405.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDADLEELLGITDHIVF
                     NSERQLRKHGARCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFEAQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            14663..15748
                     /gene="aguA"
                     /locus_tag="VW854_000093"
     CDS             14663..15748
                     /gene="aguA"
                     /locus_tag="VW854_000093"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEV0261406.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAELVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPVVRQVVTE
                     EDLPGYIYEEGEEERYAGERLAASYVNFYIANKAVLVPQFEDVNDQVAIDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            15758..16633
                     /gene="aguB"
                     /locus_tag="VW854_000094"
     CDS             15758..16633
                     /gene="aguB"
                     /locus_tag="VW854_000094"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEV0261407.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(16814..17623)
                     /locus_tag="VW854_000095"
     CDS             complement(16814..17623)
                     /locus_tag="VW854_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV0261408.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHIAAIHQAIEKGVKLVLCTGR
                     PLFGVLPYYKKLGLDLQNEYVIVNNGCSTHQTSDWGLVDWQELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKLRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            17895..18116
                     /locus_tag="VW854_000096"
     CDS             17895..18116
                     /locus_tag="VW854_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HEV0261409.1"
                     /translation="MILMTKNINLTNEELELIQGGADPYGKNPNGRYDWEIEPVLTLP
                     VHGFCPRGTYDLGYIGGGNHLCKGSAARF"
     gene            18170..18841
                     /locus_tag="VW854_000097"
     CDS             18170..18841
                     /locus_tag="VW854_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0261410.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETVEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(18893..19090)
                     /locus_tag="VW854_000098"
     CDS             complement(18893..19090)
                     /locus_tag="VW854_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0261411.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSRQSY
                     FQNQFKKISGYTPLQYRKEKR"
     gene            19083..19991
                     /locus_tag="VW854_000099"
     CDS             19083..19991
                     /locus_tag="VW854_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV0261412.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            19988..20449
                     /locus_tag="VW854_000100"
     CDS             19988..20449
                     /locus_tag="VW854_000100"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEV0261413.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            20439..21326
                     /locus_tag="VW854_000101"
     CDS             20439..21326
                     /locus_tag="VW854_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV0261414.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLALAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHSVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            21329..23224
                     /gene="cbpE"
                     /locus_tag="VW854_000102"
                     /gene_synonym="pce"
     CDS             21329..23224
                     /gene="cbpE"
                     /locus_tag="VW854_000102"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEV0261415.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDNRITNSERLWDNLYGYDKVLQT
                     ASEKGVSVIQNITQGDAHFQFGDMDIELYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHLDTKNSNTKNFIKNLSPEIIVQ
                     TSSSPIGSYDSGQEINREYISWLESMGIQHINAASKDYDATVFDIRQDGLVNISTSYK
                     PIPSFQAGWHKSSYGNWWYQAPDSTGEYAVGWNEIKGEWYYFNQTGILLQNQWKKWNN
                     RWFYLTDSGVAAKNWKKIAGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQY
                     KGQWYYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWL
                     KKANDWYFYKTDGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVP
                     IEKSATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSV
                     NKSESEQ"
     gene            23304..24434
                     /locus_tag="VW854_000103"
     CDS             23304..24434
                     /locus_tag="VW854_000103"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEV0261416.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDEVKVGDNDTLSA
                     QVAAMVQADLLVLLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            24444..25706
                     /locus_tag="VW854_000104"
     CDS             24444..25706
                     /locus_tag="VW854_000104"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEV0261417.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHEDKAASFLPRLEQ
                     VLVADRKEAGLEPIQFRLDSKASQFVSGQAAQAQDFDTEFLDYILAVKVVSSLEEAIA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVTGDGQIRE"
     gene            25710..26507
                     /gene="proC"
                     /locus_tag="VW854_000105"
     CDS             25710..26507
                     /gene="proC"
                     /locus_tag="VW854_000105"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEV0261418.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQARPSDEILLANRSQVKVDAFIAN
                     FGGQTSNNEEIFEEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSEIFCQLLAKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMEAVHQAYKRTQELGK"
     gene            26727..27365
                     /locus_tag="VW854_000106"
     CDS             26727..27365
                     /locus_tag="VW854_000106"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEV0261419.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
     gene            27362..28252
                     /locus_tag="VW854_000107"
     CDS             27362..28252
                     /locus_tag="VW854_000107"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEV0261420.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEIAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQGEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            28292..28609
                     /gene="yabA"
                     /locus_tag="VW854_000108"
     CDS             28292..28609
                     /gene="yabA"
                     /locus_tag="VW854_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEV0261421.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            28612..29481
                     /gene="rsmI"
                     /locus_tag="VW854_000109"
     CDS             28612..29481
                     /gene="rsmI"
                     /locus_tag="VW854_000109"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEV0261422.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQILKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFDS
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQGVEEKDEEDLFVEIQTRIQQGVKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            29708..30226
                     /locus_tag="VW854_000110"
     CDS             29708..30226
                     /locus_tag="VW854_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEV0261423.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            30335..30982
                     /locus_tag="VW854_000111"
     CDS             30335..30982
                     /locus_tag="VW854_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEV0261424.1"
                     /translation="MDFEYFYNREAERFNFLKVPEILVDREEFRGLSAEAIILYSILL
                     KQTGMSFKNNWIDKEGRVFIYFTVEEIMKRRNISKPTAIKTLDELDVKKGIGLIERVR
                     LGLGKPNIIYVKDFMSIFQVKENDLQKSKNLTSEVKDFNLRSKENELQEVKNLDSNYI
                     ENNKSKYSKREYSFGENGLGTFQNVFLAAEDISDLQIIMNSQLENYIRLPAKLES"
     gene            complement(30971..31775)
                     /locus_tag="VW854_000112"
     CDS             complement(join(30971..31405,31407..31775))
                     /locus_tag="VW854_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEV0261425.1"
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFEEMLTVLKTAYQLKHAKGG
                     RKPKLSLEDLLMTTLQYMREYRTYEEIAADFGIHESNLIHRSQWVEVTLVQSGFTISR
                     TPLSSEDTVMIDATEVKINRPKKQLANYYGKKKCHAMKAQTIVTSQGRIVSLDITVNY
                     CYDMKLFKMSRRNIGQAGKILADSGYQGLTKIYPQAQTPRKSSKLKPLTVEDKACNHA
                     LSKERSKVENIFAKVKTFKMFSTTYRNHRKRFGLRMNLIAGIINHELGF"
     gene            31770..32024
                     /locus_tag="VW854_000113"
     CDS             31770..32024
                     /locus_tag="VW854_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV0261426.1"
                     /translation="MHRTTIPYFVSQEVYWKVRNIEAEAIRRNCERGAIFSGKIKYHE
                     DSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            32269..33603
                     /gene="trmFO"
                     /locus_tag="VW854_000114"
     CDS             32269..33603
                     /gene="trmFO"
                     /locus_tag="VW854_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEV0261427.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVANHPLIEVVRDEITELPTDVITVIATGPLTSDALAEKIHALNDGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            33687..34424
                     /locus_tag="VW854_000115"
     CDS             33687..34424
                     /locus_tag="VW854_000115"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEV0261428.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            34433..34990
                     /gene="frr"
                     /locus_tag="VW854_000116"
     CDS             34433..34990
                     /gene="frr"
                     /locus_tag="VW854_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEV0261429.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASELGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            35050..35904
                     /gene="cvfB"
                     /locus_tag="VW854_000117"
     CDS             35050..35904
                     /gene="cvfB"
                     /locus_tag="VW854_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEV0261430.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            35913..36128
                     /locus_tag="VW854_000118"
     CDS             35913..36128
                     /locus_tag="VW854_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEV0261431.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            36214..37182
                     /locus_tag="VW854_000119"
     CDS             36214..37182
                     /locus_tag="VW854_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEV0261432.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(37160..37339)
                     /locus_tag="VW854_000120"
     CDS             complement(37160..37339)
                     /locus_tag="VW854_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261433.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLRPLDKTFSLYNWIFLSQKYI
                     LLQANFLKISRVQLQ"
     gene            37375..37875
                     /locus_tag="VW854_000121"
     CDS             37375..37875
                     /locus_tag="VW854_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0261434.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVMDFVYAYPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            37878..38138
                     /locus_tag="VW854_000122"
                     /pseudo
     CDS             37878..38138
                     /locus_tag="VW854_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(38505..39616)
                     /gene="ald"
                     /locus_tag="VW854_000123"
                     /pseudo
     CDS             complement(38505..39616)
                     /gene="ald"
                     /locus_tag="VW854_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003072563.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
     gene            39793..40362
                     /locus_tag="VW854_000124"
     CDS             39793..40362
                     /locus_tag="VW854_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0261435.1"
                     /translation="MESIFVKLAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKSNGQFIGTIDLHKIDSVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKDNPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            40430..41080
                     /locus_tag="VW854_000125"
     CDS             40430..41080
                     /locus_tag="VW854_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEV0261436.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDSSTEKEVKKEEKEEPLEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSTSKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHRETNGKFKSVDELKTVSGIGAKTIEKLKDYVTVD"
     gene            41064..43304
                     /locus_tag="VW854_000126"
     CDS             41064..43304
                     /locus_tag="VW854_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEV0261437.1"
                     /translation="MLQWIKNIPIPLIYLSFLLLWFYYAIFSASYLALLGFVFLLVCL
                     FIQFPWKSAGKVLIICGIFGFWFVFQNWQQSQASQNLADSVERVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVTSRPLVFGQPNAWFLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIEKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDKENVGDL
                     SEVTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMTVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            43493..43681
                     /locus_tag="VW854_000127"
     CDS             43493..43681
                     /locus_tag="VW854_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261438.1"
                     /translation="MTVTKSYKYDWNTVWEYSTNYHDHQYAWIPSWSRYDSYSEYKVG
                     GGWNYGRYEVINYYSGGY"
     gene            43714..44301
                     /locus_tag="VW854_000128"
     CDS             43714..44301
                     /locus_tag="VW854_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0261439.1"
                     /translation="MLQLTHVTLKTRQVILQDVDFTFKKGRVYGLLAINGSGKTTLFR
                     AISNLIPISSGNIAAPPSLFYYESIEWLDGNLSGMDYLRLIKNIWKSGLNLRDEIAYW
                     EMSDYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDVCDRVVTIHQGQIEEV"
     gene            44305..45486
                     /locus_tag="VW854_000129"
     CDS             44305..45486
                     /locus_tag="VW854_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261440.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILALFVSVLGVTFYFNSQTANSVS
                     LERELETRLVDRERVINEYEAKLSQMSDTSSEEYQFAKSNLELQKNLLKRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLSKQIDNVDLNWSMGMVLLPCLIILLLVGILFIERWGSSRKKEVFK
                     V"
     misc_feature    45646..45777
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            45793..46323
                     /gene="infC"
                     /locus_tag="VW854_000130"
     CDS             45793..46323
                     /gene="infC"
                     /locus_tag="VW854_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEV0261441.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            46356..46556
                     /gene="rpmI"
                     /locus_tag="VW854_000131"
     CDS             46356..46556
                     /gene="rpmI"
                     /locus_tag="VW854_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEV0261442.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            46608..46967
                     /gene="rplT"
                     /locus_tag="VW854_000132"
     CDS             46608..46967
                     /gene="rplT"
                     /locus_tag="VW854_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEV0261443.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            47025..47405
                     /locus_tag="VW854_000133"
     CDS             47025..47405
                     /locus_tag="VW854_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV0261444.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            47655..48455
                     /locus_tag="VW854_000134"
     CDS             47655..48455
                     /locus_tag="VW854_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEV0261445.1"
                     /translation="MNPTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHGRGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            48466..49404
                     /locus_tag="VW854_000135"
     CDS             48466..49404
                     /locus_tag="VW854_000135"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEV0261446.1"
                     /translation="MTTNRLQVSLPGLDLKNPIIPASGCFGFGQEYAKYYDLDLLGSI
                     MIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAEKLPWLEREYPNLPIIA
                     NVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGLLIGQDPDLAYDVVKAA
                     VEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVGMRFDLKTRKPILANGT
                     GGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEMYLAGASAIGVGTANFT
                     NPYACPVIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            49446..51479
                     /locus_tag="VW854_000136"
     CDS             49446..51479
                     /locus_tag="VW854_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEV0261447.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKEKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKIAEKEWVYDS
                     YSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKIAEKEWVYDSHSQAWYYFKSGGYM
                     TANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLGSD
                     GKWLGGKATNKNAAYYQVVPVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAIT
                     ISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIPVASHLSDMEVGKKY
                     YSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAE
                     EHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILG
                     ATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(51540..53195)
                     /gene="pavA"
                     /locus_tag="VW854_000137"
     CDS             complement(51540..53195)
                     /gene="pavA"
                     /locus_tag="VW854_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEV0261448.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTESLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQENKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            53300..53797
                     /gene="ybeY"
                     /locus_tag="VW854_000138"
     CDS             53300..53797
                     /gene="ybeY"
                     /locus_tag="VW854_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEV0261449.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRNTDRPTDVISLEYKPELEIAFDEEDLLENSELAEMMSEFDAYIGEL
                     FISSDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            53778..54173
                     /locus_tag="VW854_000139"
     CDS             53778..54173
                     /locus_tag="VW854_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEV0261450.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFALTGIFTAIKEERNMRKHAVTAL
                     VVILAGFGFQVSRIEWLFLLLSIFLVVAFEILNSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            54190..55089
                     /gene="era"
                     /locus_tag="VW854_000140"
     CDS             54190..55089
                     /gene="era"
                     /locus_tag="VW854_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEV0261451.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMLREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            55138..55962
                     /gene="mutM"
                     /locus_tag="VW854_000141"
     CDS             55138..55962
                     /gene="mutM"
                     /locus_tag="VW854_000141"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEV0261452.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQIHPARPSQTLTAEEATAIHDQTIAVLGQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            55962..56567
                     /locus_tag="VW854_000142"
     CDS             55962..56567
                     /locus_tag="VW854_000142"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEV0261453.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQAVDADAVVHQLQKPGG
                     RLFEALVQHFGQEIILENGELNRPLLASLIFSNPEEQKWSNQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            56554..57753
                     /locus_tag="VW854_000143"
     CDS             56554..57753
                     /locus_tag="VW854_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter PmrA"
                     /protein_id="HEV0261454.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            57753..57902
                     /gene="rpmG"
                     /locus_tag="VW854_000144"
     CDS             57753..57902
                     /gene="rpmG"
                     /locus_tag="VW854_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV0261455.1"
                     /translation="MRVKINLKCSSCDSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            57942..58175
                     /gene="secG"
                     /locus_tag="VW854_000145"
     CDS             57942..58175
                     /gene="secG"
                     /locus_tag="VW854_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEV0261456.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            58277..60631
                     /gene="rnr"
                     /locus_tag="VW854_000146"
     CDS             58277..60631
                     /gene="rnr"
                     /locus_tag="VW854_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEV0261457.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEAKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTIIQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVPSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSHSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            60594..61061
                     /gene="smpB"
                     /locus_tag="VW854_000147"
     CDS             60594..61061
                     /gene="smpB"
                     /locus_tag="VW854_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEV0261458.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            61077..61937
                     /gene="tehB"
                     /locus_tag="VW854_000148"
     CDS             61077..61937
                     /gene="tehB"
                     /locus_tag="VW854_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEV0261459.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            62010..62963
                     /gene="coiA"
                     /locus_tag="VW854_000149"
     CDS             62010..62963
                     /gene="coiA"
                     /locus_tag="VW854_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEV0261460.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            62982..64784
                     /gene="pepF"
                     /locus_tag="VW854_000150"
     CDS             62982..64784
                     /gene="pepF"
                     /locus_tag="VW854_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV0261461.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQIEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGIYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYVDLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            64786..65499
                     /locus_tag="VW854_000151"
     CDS             64786..65499
                     /locus_tag="VW854_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEV0261462.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGSLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            65566..66507
                     /gene="prsA"
                     /locus_tag="VW854_000152"
     CDS             65566..66507
                     /gene="prsA"
                     /locus_tag="VW854_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEV0261463.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(66623..66964)
                     /locus_tag="VW854_000153"
                     /pseudo
     CDS             complement(66623..66964)
                     /locus_tag="VW854_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            67377..67472
                     /locus_tag="VW854_000154"
                     /pseudo
     CDS             67377..67472
                     /locus_tag="VW854_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(67725..68345)
                     /locus_tag="VW854_000155"
     CDS             complement(67725..68345)
                     /locus_tag="VW854_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV0261464.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLHTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(68345..68827)
                     /locus_tag="VW854_000156"
     CDS             complement(68345..68827)
                     /locus_tag="VW854_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEV0261465.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDRMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(68827..69669)
                     /locus_tag="VW854_000157"
     CDS             complement(68827..69669)
                     /locus_tag="VW854_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEV0261466.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDFKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(69764..70564)
                     /locus_tag="VW854_000158"
     CDS             complement(69764..70564)
                     /locus_tag="VW854_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEV0261467.1"
                     /translation="MRKQIHPLILFSFFGIMMFILIINRPLNDNQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            70708..72087
                     /gene="glmU"
                     /locus_tag="VW854_000159"
     CDS             70708..72087
                     /gene="glmU"
                     /locus_tag="VW854_000159"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEV0261468.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDIEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            72097..72642
                     /locus_tag="VW854_000160"
     CDS             72097..72642
                     /locus_tag="VW854_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV0261469.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            72654..72968
                     /locus_tag="VW854_000161"
     CDS             72654..72968
                     /locus_tag="VW854_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEV0261470.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            72985..73677
                     /locus_tag="VW854_000162"
     CDS             72985..73677
                     /locus_tag="VW854_000162"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEV0261471.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNAQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            73754..74245
                     /gene="rocS"
                     /locus_tag="VW854_000163"
     CDS             73754..74245
                     /gene="rocS"
                     /locus_tag="VW854_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEV0261472.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            74343..74933
                     /locus_tag="VW854_000164"
     CDS             74343..74933
                     /locus_tag="VW854_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEV0261473.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            74942..76705
                     /locus_tag="VW854_000165"
     CDS             74942..76705
                     /locus_tag="VW854_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEV0261474.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT        23125 a        14089 c        17097 g        22558 t
ORIGIN      
        1 ttttgagcag cctgcggcta gcttcctagt ttgctttttg attttcattg agtatgaaaa
       61 ggattggaga aagccttcca gcttgtggta taatagattt atggataaaa aatatgaaaa
      121 aatctctcag gatttgggag tgacgttaaa gcaaattgat accgttctaa gtttgacagc
      181 tgaaggggcg actattccct ttatcgcgcg ttatcgcaag gacatgactg gtagtctgga
      241 tgaggtggcg attaaggcta ttattgattt ggataaaagt ctgacaaatc tcaatgaccg
      301 taaggaagct gtcttagcta agattcaaga acaaggtaag ttgaccaagg aattggaaga
      361 agctatctta gttgccgaaa aattagcaga cgttgaagaa ctctatcttc cttataagga
      421 aaagcgtcgt accaaggcaa ccattgcccg tgaagctgga ctctttcctc ttgctcgctt
      481 gattttgcag aatatagttg acttagagaa agaagctgaa aagttcgtct gtgaaggatt
      541 tgcgactggc aaggaagcct tgaccggtgc agttgatatt ttggtcgaag ccttatcgga
      601 agatgtgacc ttgcgttcta tgacttatca ggaagtgctg agacactcta aactcacttc
      661 tcaagccaag gatgaaagtc ttgatgaaaa gcaggttttt cagatttatt atgatttttc
      721 agagacagtt ggaactatgc aaggctatcg taccttggct ctcaatcgtg gggagaaact
      781 tggtgtcttg aagatcggtt ttgaacatgc gacggaccgt attcttgcct tctttgctac
      841 tcgtttcaag gtgaaaaatg cttatattga tgaagttgtt cagcaatccg ttaagaaaaa
      901 ggtcttgcct gctattgagc gtcgtattcg gacagaatta actgagaaag ctgaagaggg
      961 agctatccaa cttttttctg acaatctgcg caatctcctc ttggttgctc cactgaaagg
     1021 gcgcgtggtt cttggatttg acccagcctt tcgtacaggt gccaagttag ctgtcgtgga
     1081 tgcaacagga aaaatgctga caactcaggt tatttatcct gttaaaccag catcagctcg
     1141 tcaaatcgaa gaagccaaga aagatttagc agatttaatt ggtcaatacg gtgtagagat
     1201 tattgccatt ggaaatggaa cggccagtcg tgaaagtgaa gcttttgtag cggaagttct
     1261 gaaagatttc cctgaagtca gctatgttat cgttaatgaa agtggtgctt ctgtctattc
     1321 tgccagcgaa cttgctcgtc aggagtttcc agacttgacc gttgaaaaac gctctgccat
     1381 ttctatcgcc cgtcgtttgc aagatcctct tgcggaattg gtcaaaatcg atcctaagtc
     1441 aattggtgtc ggtcaatacc aacacgatgt cagtcagaag aaactatctg agagtctgga
     1501 ctttgttgtc gatacagtgg ttaaccaagt tggtgtcaat gtcaatacag ctagcccagc
     1561 tcttctttca cacgtagctg gactcaacaa aactatctct gaaaatattg tcaaataccg
     1621 cgaggaagaa ggaaaaatca cttcacgcgc ccaaatcaag aaagttcctc gtctgggagc
     1681 caaggccttt gagcaggctg ctggtttcct tcgtatccct gaaagtagca atatccttga
     1741 taatacagga gttcacccag agaattacac tgccgttaag gaactcttca aacgtttaga
     1801 tattaaagac ctaaacgaag aagcccaaag caaactcaag tccctttcag tcaaggaaat
     1861 ggctcaagag ctagacctcg gtccagaaac ccttaaagat atcattgcag atcttctcaa
     1921 accaggtcga gatttccgtg attcctttga cgcacctgtg cttcgccaag atgtcctaga
     1981 tatcaaagac ctagtggtag gtcagaagct agaaggtgtt gtgcgtaacg ttgttgactt
     2041 cggtgccttc gttgatatcg ggattcacga ggacggcttg attcatattt ctcatatgag
     2101 tcgcaagttt atcaaacacc ctagtcaagt ggtggcagtt ggagatttgg tgaccgtttg
     2161 ggtcaagaaa attgatactg aacgtgaaaa agttaatctg tcgctcctcg ctccaaatga
     2221 aactgactga ttatgtcaag caggtttcac tagaagactt cggcagacct tttatccatc
     2281 atgtccagtg gaataggcgt ctacgttcga caggtgggcg atttttcccc aaagatgggc
     2341 atctggattt taatcccaag gtttatcaga aactgggtat ggaggttttt aggaaaattg
     2401 tccgacatga actctgtcat tatcaccttt atttccaagg gaagggctat caacacaagg
     2461 atcgggattt taaggaactt ttgaaagcag tggatggatt acggtttgta ccatccttgc
     2521 ccaatagtaa ctctaaacca ctcaagcttt atcgttgtca atcctgccag caaaggtatc
     2581 agcgcaagcg taggattgat accaaacgct atcgctgtgg actttgtcga ggtaaattgc
     2641 ttctgataaa tcagcctgag gactgatgaa agccgagcct ccccgtgata tactatgtct
     2701 aacctagcag aaagaggaaa tatcatgaac agtaaatttt ataaaatgag acgcaatcgc
     2761 atgatttcag gagttctggc aggtctgtca gacaagtgga attttgatgt taccctagtt
     2821 cgtttcttgt ttgctatttt tacagttgca aactttggac ttggcgtgat tatctatatc
     2881 atcctagctt ctatcatgcc taccaaggaa gaaatcgaag cagaaatgta cggaacaggc
     2941 ccacgtaaac gcaaagaagc ccaagccatt gacgacaatg aaggctggtt ttggtgaggt
     3001 tctacagaag atttggaata agcaaaaagc tttaaatcaa tagtctaaac tgcttgattt
     3061 aaagcttttt tacttttata ctcaataaaa atcaaagtgc aaactaggaa gccagccaca
     3121 ggctgtaatt tagtcggcaa tgtgaagttg acatagtttg cactttgatt tttgaatagt
     3181 attaataata tttcgccagc ttcatccaca aactatagtg gattggaact agaataggac
     3241 acctctactt ctaaaacatt gttagaaatc gatttgacta tcctgatcga tttgtcctgt
     3301 tcttatttca ttttattata aaagcagtaa tttaaactca gttttttaat ctatttttgc
     3361 tgctgagaca atacattttt ggaaaaatgg tggatgctga taacaactga taaccagcaa
     3421 ataacataag cagtgccggt aaattctgta ccgataaagt gtgtgtgttc aactaagagc
     3481 tgaccgatga atggagagag cagatagaga agtccgatga gtatatgttc tttgtgtttc
     3541 attaaagcgc accttctttc cttataggtt gaacgacttg caagtgttaa attgaatggt
     3601 atgttatcta ggtaacctta ctaaaatgtt agttcccgct tcctcctttc aaagtaagcc
     3661 ttttcacact tatattttac aactttaaaa ataggaaatc aaatcatttt ctaagtaatt
     3721 ttgcttatat ttcacccaat cttacaaaaa tgtaagatta aagtctcttt tgtaaggtta
     3781 ggttatggca agcggtcttt ttttgatgta aactagactc ataaagaaca aaggagtaac
     3841 atcatgaaac aaatcttaca taagaaaact agaaaaccaa gtcacaaaga aattgagcgc
     3901 gttcagctgg gatgcactat gatgcaagct acatttcatt tgatgggata ttaagaaagg
     3961 agattctcat gacactttta gatgtaaaac acgttcaaaa aatttataaa acacgttttc
     4021 agggcaacca agtagaagcc ctcaaggata ttcactttac cgtcgaaaag ggtgactacg
     4081 ttgccatcat gggtgagtct ggttctggta aatcaactct tctcaatatt ctagctatgc
     4141 tggataaacc aagtcgtggt caggtttact tgaatggaac tgacaccgca accattaaaa
     4201 attcacaggc ctctagcttc cgtcgtgaaa agttgggctt tgtcttccaa gactttaact
     4261 tgctagatac tctatctgta aaggacaata tcttgcttcc gcttgtcttg tcaagaagac
     4321 ctattacaga gatgatgaag aaattggtgg tgacagctga gaatctggga ataaaccaat
     4381 tacaagagaa gtacccttac gagatttctg gtggtcagaa acagcgtgta gcagtagccc
     4441 gcgccatcat cacagaacct gaaattctcc ttgcggacga accgacagga gctcttgact
     4501 ccaagtcatc tgcagcctta cttgatgtct ttgatgaaat caatgagcgc ggccaaacca
     4561 ttctcatggt aacccactca acagcagcag ctagcagagc taaacgtgta ctctttatca
     4621 aagacggcat tctttacaat caaatctacc gtggagagaa gacagaacgt cagatgttcc
     4681 aagaaatctc tgataccttg actgtcatgg caagcgaggt gaattagtat gtttcgatta
     4741 accaataagt tagcggtatc gaacttgatt aaaaaccgca aactctacta tccttttgcg
     4801 ctggctgttc tcttggcagt cactgtcacc tatctctttt actctctaac cttcaatcct
     4861 aagattgcgg aaatccgtgg aggaacaacc attcaggcta cacttggatt tggtatgttt
     4921 gtcgtcaccc ttgcgtcagc cattatcgtt ctctatgcca atagttttgt catgaagaac
     4981 cgttccaagg aactaggaat ttatggcatg ttgggcttgg agaagcgtca tcttatcagt
     5041 atgaccttta aggagttagt ggtatttggg attctaactg ttggagcggg tatcggtatt
     5101 ggagccttgt ttgacaagtt aattttcgct ttcctgctca aactaatgaa attgaaggtt
     5161 gagctggttg ctaccttcca gacgaaagtt gtcattacag tgcttgttgt cttcggtttg
     5221 attttcctag gcctcatgtt cctgaatgcc cttcgaatcg cccgtatgaa tgccctccag
     5281 ctctctcgtg agaaagctag tggagagaaa aaaggtcgct tccttcctct ccaaaccatt
     5341 cttggttcca taagtttagg aattggctat tatcttgccc ttacggtaaa agatcctctt
     5401 acagccttaa caaccttctt catagctgtt ttactggtta tctttgggac ttatctcttg
     5461 tttaatgcag ggattaccgt tttcctccaa atcttaaaga aaaataagaa atactattac
     5521 caaccaaata acctcatatc tgtttctaac ttgattttcc gtatgaagaa aaatgcagtt
     5581 ggactagcca ccatcgctat cttgtcaaca atggttttgg taaccatgtc agcagcgaca
     5641 agcattttca attcctcaga aagctttaaa aaagttctaa atcctcatga ttttggggtt
     5701 tcagggcaaa atgttgaaaa agaagacttg gacaaactct tgagccagtt tgcaagtgac
     5761 aaaggttata agattaaaga aaaagaagta tttcgttaca cttactttgc tgttgcgaat
     5821 caagaaggaa ccaagttaac tatttttgaa aaaggacaaa accgtgtcca accaaaaaca
     5881 gttttcatgg tatttgacca aaaagattat gaaaatatga ctggtcaaaa actgtctcta
     5941 tcaggaaatg aggtcggact ctttgcaaag aatgagggag ttaaagaaca gaaagctcta
     6001 actctaaatg atcatcaatt ttctgtaaaa gaagaattta ctaaagattt tattgtcaac
     6061 catgttccaa atcagtttaa tattttgact gctgattaca attaccttgt tgtacctgat
     6121 ttacaagcct ttttggatca attcccagat tcggctatct ataatcagtt ttacggtggt
     6181 atgaatgtaa atgccagtga agcagaacaa ctcaaggtcg ctgaggagta tgaaaaatac
     6241 ttacaaaagt ttaatgctca attaaacact gaaggtaact atgtgtatgg tagcactcta
     6301 gcagatgcta gtgctcagat gagtgccctc tttggtggtg tcttctttat cggtattttc
     6361 ttatccatta tctttatggt cggaaccgtt ctggtcatct actacaaaca aatttctgaa
     6421 ggatatgaag accgtgagcg ctttattatc ttgcagaaag tcggtttaga tcaaaagcaa
     6481 atcaagcaaa ccatcaacaa acaggtttta actgtattct tccttccttt gctctttgcc
     6541 ttcctacatc tagcctttgc ctaccatatg cttagtctga ttttaaaagt gattggtgta
     6601 ctggatacga ctatgatgtt gattgtgacc ttgtctatct gcgctatctt cctcatcgcc
     6661 tatgtgctga ttttcatgat tacttcaaga agttatcgta agattgtcca aatgtaaaaa
     6721 aagatacctc gacttcaaaa tcgaggtatt tcttgttttc taaatgctga aaagttgtcc
     6781 gagcagaaag gtaactccca tagttaagag accaatagca aggttccgaa tcatagctgt
     6841 tttggttggg gcttttccaa gtctagcact tgtgtagcca gtgagaagaa gggctacacc
     6901 gacgataagg acggtagcag ggatgcggta atcacttgga aaaatggtca ctgacagcat
     6961 tggaggcaaa cttctaagga aaaaggcaac gaagctagaa atggcagcgt gccaaggatt
     7021 ggtaaattct tcatactcaa tcccatattt ttcctctacc agagccttga gtggattttt
     7081 aagaaagatc ttattagtca agagttgggc agaagttttg aattctccat tttggatata
     7141 agaagcatag agggattttt tggctagttc cctatcttgg tctagcaaga gtttttctcg
     7201 cgaaacggca gcttcctcgg tatcttttgg agttgaaacg gatacatatt ctccaccagc
     7261 cattgaaaag gcaccagcta agatagccgc aaaacctgat aaaaagataa tccagatatt
     7321 gctcgtggca ctggcaactc cgataaccac accagcaatg gaaataattc catcgttagc
     7381 atcaagaaca cccgcacgca ggatatttaa acgacctgca aaatttgaat caatttcgtg
     7441 atttgtttct gacgctaaat ttcaagttca agttagccat ccagaagtct tctctgggtg
     7501 acttgtagtc caagcatttt ttaggatagt tgttaatcca cttttcgatg aatgcgactt
     7561 ctttgggagt cattttcttg gttcccttag gtaaccatct acgaatgagc ctgttgtgat
     7621 tctcattagt tcccctttcc caagaggcat aggggtgcgc ataatagatg tgctcctcag
     7681 aaaatacatc aaacaagcga ttgaattccg ttccattatc tgccgtgatg gaaagaatct
     7741 tgtgttgttt taagatgagt tttagagcct gattgaccac ctcagcactt ttatttggaa
     7801 tcaatcggat gatctgatgt ctactctttc gatccgtcaa gacaagcaag cagtagtttt
     7861 tcgctctcgt aagtagaacc gtatcaatct cataataccc attctccaag cgaagattga
     7921 tagcttcaga ccgctgttcg atggattgac cagcaggttt aaagttggtg ctagcctgtt
     7981 tcttatcagg cgaaaatttt tggttatgat agtggagaat cttttccttt agttccttgg
     8041 tcaagcttga tttcttgacc gagcgcttgc gattgttttc ataagactgt tgagcatagt
     8101 cggcagaata aacctctttg aagcgccctt ttccaagaca ttgtcggact gtcccacgct
     8161 tgatttcagt gtggatagtt tgaggagctt ttccaagtag agaggcattt tctctatttg
     8221 atttcccttc ttttttccat ctttcgatta agcgacggct atcgattgtc aaatgtttgg
     8281 cttttgtagt ataattgtct tgcatctctg tgcctttctt gtgtttgtgg ttgaacaaca
     8341 agtataacac agaggtgttt tcttatacct acaagagcta tcagctagtt gaaccatcta
     8401 atttttagga gggctgggtg gctaacttca ttatagaact ttcatttacg aacatatagt
     8461 aaaatgaaac aagaacagaa caaatcgatc aggacagtaa aatctatttc taacaatgtt
     8521 ttagaagcag aggtgtacta ttctagtttc aatctactat atttttgttt tttatcaaaa
     8581 aatactttac aagttcttaa aaacatgata tagtaataaa gcttagaaaa tgagatgatg
     8641 ttttctagca aatataaacc cgagtaaaaa atgcctacgg acaggcaggg ttgaatgccg
     8701 aagcgtggtt gaaaagccac attattgata gggttaaaag cctactttta taagttgatg
     8761 ttaggacact tgtcctaatt cataaatttt tagtgtggtg aaagcacacg tcatcttgtg
     8821 aaacgatcaa taaagtacgt aatatttgct actagagagt taggaaacat cgggaacaga
     8881 catactcaac agaaaccaaa ataaacacgt cagaagattg cagagcaggt gaaaacctgc
     8941 tcttttttca tgagtcaacc tttagttcct tagttttcat aaggtcctaa aaatattgaa
     9001 aggagtatgt tttgaaagag ttagatcaaa accaagcccc aatttatgag gccttggtga
     9061 agttacgcaa gaaaaggatt gttccctttg atgttccagg tcacaagcgt ggacggggaa
     9121 atccagaact tgtcgaactc ttaggagaaa aatgtgtagg cattgatgtc aattcgatga
     9181 aacccttgga taatttaggc catcctattt cgattattcg tgatgcagag gagctggctg
     9241 cagatgcttt tggagctagc catgcctttc taatgattgg tggaacaact tcatcggtgc
     9301 agactatgat tctggcaacc tgcaaggcag gagataagat tattctgcca cgaaatgtcc
     9361 ataaatctgc tatcaatgcg ttggttctat gtggtgccat tcccatctat atcgagatga
     9421 gtgtagatcc taagattggt atcgctttag gtcttgaaaa tgaccgagta gcacaggcca
     9481 taaaggacca tccagatgct aaggctatcc taatcaacaa tcctacttac tacggcatct
     9541 gttcagacct aaaggggttg acagaaatgg ctcatgaagc tggcatgatg gttttagtag
     9601 atgaagccca cggagcgcat ttgcatttca ctgataaact tccaatttct gctatggatg
     9661 caggggctga tatggcagca gtttccatgc ataagtctgg tgggagtttg acccaaagct
     9721 ccattttact tatcggggag cagatgaatt ctgaatacgt tcgtcagata attaacctga
     9781 cccagtctac atctgcctct tacttgttga tggctagttt ggatatttca cgtcgcaact
     9841 tggcccttcg tggtaaagag tcgtttgagg aagtcattga gctatctgag tatgcccgcc
     9901 gtgaaatcaa tgctatcggt ggctactatg cctactcaaa agagttaata gacggtgttt
     9961 cggtttgcga ttttgacgta actaagctgt cagtttacac tcagggtatt ggcttaacag
    10021 gtatcgaggt ttatgacctc ttgcgagacg aatacgacat tcagatcgag tttggtgata
    10081 tcggcaatat cttggcctat atttccatcg gcgaccgcat ccaagacatc gagcgcttgg
    10141 ttggtgctct ggctgatatt aagagactct attcaagaga tggaaaagat ttgatagcag
    10201 gagaatatat tcagcccgag ttagtgctgt ctccgcaaga agccttctat tcagaaagaa
    10261 aaagtttgac cttagatgag tctgttggac aggtctgtgg agaatttgtc atgtgctatc
    10321 ctccagggat tcctatcttg gctcctggtg aacgtattac acgagaaatt gtagactata
    10381 tccaattcgc caaggaacgt ggttgctccc tccaagggac ggaagatcca gaggtcaatc
    10441 atatcaacgt tattaagaga aagacaaact ataagaaaag tcaatagttt tatctaaact
    10501 atttcttatt tcaatttgat gatttggcga tgattttaga gcacggcaaa aagcccttga
    10561 attagaagcg gtcaatcgct taatttctat cagcttatca aatcctgcct caatcctttt
    10621 ctgaggatta gggtagcgtg tcaagagttg gtaggtatat tctgaatgct ttccaacgat
    10681 tttatccaac tcaggaaaga tgatatcaag acaacgagtg tattgtactt tccaatcaga
    10741 ctgtttttct tgagacgatg aatatgtcta gccagtattt ttaggtctac ttgccgatta
    10801 tcgtgttgaa attgttcacg attggggtca gaaagaagtt taagagcgat gccatgagcg
    10861 tctttcttat ccgttttagt cttgcgaagt gataatgatt tggcaaattc cttgatgagc
    10921 aaaggattgt aggtgtaaac tttatatcct tgttcatgca ggaagttcag tagattaaag
    10981 gcataatgtc cagtatcttc aagagcgatg agacagtctt ggttgagctg tcgaagagac
    11041 agatctaaga gttcaaaacc agctttatta tttgaaaaag tgagtggttt aagaacagtt
    11101 tttccttgaa cattcaaggc tgtaacatcg tgtttatttt tagcgatatc aatgcccaca
    11161 taaagcatgg gagtacctcc agatgtagta tttcaagtct acttggttat ccacgaactt
    11221 tttgccttgt taccttagac gagataaaac gtctatgcgt tatcaaactc attaccaatt
    11281 gaaacaaaag ctgtggttag agcctttcgg aaatcgtcaa gcgattggag gaaatgaact
    11341 aatccacagt ggcttattcc aagtatacca cttgggcttt ggcagtagct aactgcgcta
    11401 aatataatat aaggagaaat agatggattt atggttttct gaagttcata ctccagatgt
    11461 caaattgtct ctgagaacag ccaagcaact ttacgctgga aaaagtgaat ggcaggatat
    11521 cgaagtcttg gatacgccag cttttgggaa aatactgatt ttaaatggcc atgtcttgtt
    11581 ctcagatgcg gatgatttcg tctacaatga aatgaccgtt cacgttccca tggctgtcca
    11641 cccaaatcca aagaaagtat tggttattgg gggtggtgac ggcggtgttg cccaagtatt
    11701 aacactctat cctgaactgg aacaaatcga tatcgtggaa ccggatgaga tgttggtcga
    11761 ggtctgtcgt gagtatttcc cagactttgc tgcagggcta gatgatcctc gtgttaccat
    11821 ttactaccaa aatgggctac gctttttgcg aaactgcgaa gatgattacg atattatcat
    11881 caacgatgcg acagatccat ttggccatac ggaaggactc tttaccaagg aattctacgg
    11941 caatagttat cgagctctga aggaagacgg catcatgatt tatcagcatg ggagtccctt
    12001 ctttgacgag gatgagtcgg cctgccgaag catgcaccgc aaggtcaatc aatcctttcc
    12061 aatcagtcgg gtttatcagg cccatattcc aactagccca gctggctatt ggttgtttgg
    12121 atttgcatcg aaaaaatacc accctgtcaa agattttgac aaggaaggtt ggaaaaaacg
    12181 ccagcttttc acagaatact acactgcaaa cttacacgtg ggagccttta tgttgcccaa
    12241 gtatgttgag gacattttag aagaagagga aggaaaaaaa tgagtcgttt actagttatt
    12301 ggttgtgggg gcgttgccca agttgctatt tcaaagattt gtcaagatag cgaaacattt
    12361 acagagatta tgattgctag ccgtaccaag tcaaaatgtg atgacttgaa agctaaactg
    12421 gaaggcaaaa caagtacaaa gattgagaca gctgctcttg atgctgacaa ggttgaagaa
    12481 gtgattgctc tgattgaaag ctacaaacca gaagctgttt tgaatgtagc tctgccttat
    12541 caagatttaa ccattatgga tgcttgtttg gcaacaggtg ttcactatat cgatacagcc
    12601 aactacgaag cagaagacac agaagaccct gagtggcgtg ctatctacga aaaacgttgt
    12661 aaggaacttg gttttacagc ctactttgac tactcatggc agtgggctta tcaagagaaa
    12721 ttcaaagaag caggcttgac tgctcttctt ggttctggtt ttgacccagg tgtaactagt
    12781 gtcttttcag cttatgccct caaacactat tttgatgaaa tccattatat cgacatttta
    12841 gactgtaatg gcggtgacca cggttatcca tttgcaacca actttaatcc agaaattaat
    12901 ctccgtgagg tttctgcgcc gggttcttac tgggaagatg ggaaatgggt cgaagtcgaa
    12961 gctatgtcta tcaagcgtga gtatgatttc cctcaagttg gacaaaaaga tatgtatctc
    13021 cttcaccatg aagaaatcga atcattggcc aagaacattc caggtgtcaa acgcattcgt
    13081 ttctttatga cttttggtca atcttacttg acgcacatga aatgtcttga aaatgttgga
    13141 ctccttcgta cggataccat taactttaac ggccaagaaa ttgttccaat tcaatttttg
    13201 aaagccttgc ttctagatcc tgctagcctt ggcccacgta ctgtaggtaa aaccaatatt
    13261 ggatgtatct ttacaggtgt caaagacggt gtcaaaaaga ctatctatat ctacaatgtc
    13321 tgcgaccatc aggaatgtta cgcagaggtt ggttcgcaag ctatttctta tacgacagga
    13381 gttccagcca tgattgggac aaaattagtc atgaacggaa cttggaaaca agctggagtg
    13441 tataaccttg aggagttaga tccagatcca ttcatggaag ctttgaatga gtatggtttg
    13501 ccatgggttg tggttgaaaa tccacaaatg gtggactaat gaagttagaa caagtaccaa
    13561 caccagccta tgttattgac ttggccaagt tagaagctaa ttgccgcatt ctacaatatg
    13621 tacaagaaga ggccggttgc aaggtcttgc ttgcccagaa ggcatattcc ctctacaaaa
    13681 cttatccctt gattagccag tatctatcag gtacgacagc tagtggactc tatgaggcca
    13741 aattggcaag ggaagaattt cctggtgaag tccatgtatt tgcgcctgct ttcaaggatg
    13801 cagacttgga ggaattgctt gggataacag accatatcgt ctttaactca gagagacagt
    13861 tgcgtaagca tggggctcgt tgtcgagagg ctggtgtcag tgttggtttg cgcctcaacc
    13921 ctcagtgttc aactcaagga gatcacgcgc tctatgaccc ttgtgcacca ggttctcgct
    13981 ttggagttac tatagacaag attccgagtg atttgctaga tttggttgac ggacttcatt
    14041 ttcataccct ttgcgagcag ggagcagatg atttacaaac aactttgaaa gcagtagaag
    14101 aacaatttgg tccctactta catgaggtaa aatggctcaa tatgggtggt ggtcatcata
    14161 ttacaagaga aggttacgat gtggatttgc tgatttcaga aatcaagcgt atccgaaaaa
    14221 cttacaatct tgaaatctat atcgagcctg gtgaagccat tgcgcttaat gcgggttatt
    14281 tagcaactga ggtattagat attgtagaaa acggtatgga aatcttggtt ttagacgcct
    14341 ctgcgacctg ccatatgcct gatgtacttg agatgcccta tcgtccacct ttgagaaatg
    14401 gctttgaggc acaggaaaaa gcccatacct acagactttc ttctaatacc tgtctgactg
    14461 gcgatgtgat tggtgattat agttttgaaa atccagtcca aatcggagac agactttatt
    14521 ttcaagacat ggccatttat tcttttgtca aaaataatac ctttaatggt attggattgc
    14581 caagtctcta tctcatggac gaacagggag actgtagctt actcaaagct tttggctatc
    14641 aagactttaa agggagatta tcatgatgga cagtccaaaa aaattaggct atcacatgcc
    14701 agcagagtac gaaccccatc atggtaccct catgatatgg ccgactcgac caggatcatg
    14761 gccttttcaa ggaaaggctg ctaaaagagc atttactcag atcatcgaga ccatagcaga
    14821 aggggaaaga gtctatcttt tggtggagca ggcctatcta tctgaagccc aatcctatct
    14881 tggagacaag gttgtttatt tagacattcc caccaatgat gcctgggcgc gtgatactgg
    14941 cccaaccatt ctcgtcaatg ataaaggtaa gaaattagcc gtggattggg ccttcaatgc
    15001 ttggggaggc acctatgatg gtctttatca agattatgaa gaggatgacc aagtagccag
    15061 tcgttttgct gaggctttgg aaaggcctgt ctatgatgct aaaccttttg tactggaagg
    15121 aggcgcaatc catagcgatg gtcaaggaac tattctcgta actgaaagtt gcttgcttag
    15181 tcctggtcgc aatcctaact tgactaaaga ggagattgaa aacacattat tagaaagtct
    15241 tggtgctgaa aaagttattt ggcttcctta tggtatttat caggatgaaa ccaatgaaca
    15301 cgtcgataat gttgctgcct ttgttggtcc tgctgagctt gttttggctt ggacagatga
    15361 cgaaaatgat ccccagtatg ccatgtcaaa agcagatctc gaactcttag aacaggaaac
    15421 agatgcaaaa ggttgtcact tcaccattca taaattgcct atccctgtag ttcgacaagt
    15481 tgtgacagaa gaagatttgc caggctacat ctatgaagaa ggagaagaag agcgatacgc
    15541 aggtgaacga ctagcagctt cctacgtaaa cttttatatc gccaacaagg ctgtcttggt
    15601 tccacagttt gaggatgtaa acgaccaagt ggccatagat atcctcagca agtgtttccc
    15661 agaccgtaaa gttgtcggaa taccagccag agatattctc ttaggtggtg gtaatatcca
    15721 ctgtatcacc caacaaattc cagaatagga gaaaaagatg agaaatgtaa gagttgcaac
    15781 cattcagatg caatgcgcta aggatgtggc aacaaatatc caaaccgcag agcgtttagt
    15841 acgtcaggct gctgagcaag gagcccaaat tattctcttg cccgagttgt ttgaacatcc
    15901 ctatttctgt caggaacgtc agtatgacta ctaccagtat gcccaatctg tagcggaaaa
    15961 tactgccatt cagcatttta aggtgattgc taaggaacta caagttgttt taccaatcag
    16021 tttctatgaa aaagatggta atgtcttgta taactctatt gccgtcattg atgcagatgg
    16081 ggaagtgctg ggcgtttatc gaaagaccca cataccagat gaccattatt atcaagaaaa
    16141 attctatttc acgcctggta acactggttt caaggtctgg aatactcgct atgctaagat
    16201 tggtatcggt atctgttggg atcaatggtt ccctgaaaca gcgcgctgtc ttgcattgaa
    16261 tggtgctgaa ttgctctttt atcctacagc tatcggttca gagccaattt tggatacaga
    16321 tagttgtggt cactggcaac gtactatgca agggcacgca gcagcgaata ttgttccagt
    16381 catcgcagcc aatcgttatg gtttagagga ggttactcct agtgaggaaa atggcggaca
    16441 gagttccagt cttgacttct acggttcctc ctttatgacg gatgaaacag gagctattct
    16501 agaacgagct gaaagacaag aagaagctgt tctgttagct acttatgacc tagacaaggg
    16561 agcaagtgaa cgcctaaact ggggcttgtt tcgagataga agaccagaaa tgtatagaca
    16621 aattacagat tagtgtggga gaaatgagag attcattctg ctagactaac ttcttattag
    16681 caactataag atactatggc atctagtaaa tcgattttta tgattcgcta ttcttgtcta
    16741 ttgattagtc cgtattttaa aatattagca aaaaagcaaa tagcagtaac ttctgtctat
    16801 ttgcttttct tttttataga atatatttct caatagcacg cgcaacgccg tcttcttcgt
    16861 tgcttgaggt aacggcatcc gcaagagatt tgacataatc gctggcattt cccattgcaa
    16921 tcccaagccc tgcaaactgg agcatttcga tatcgttatt agcatcgccc atggccataa
    16981 tctctgagga atcaatcttc aaaatctcag ctagtcgtga aagagcagta gcctttgtcg
    17041 ttccaagcgg cattgcttca taaatgacag gctgcgaacg aactccactg aatcgttggc
    17101 aaagctcttc agcaaaacgc tgctcaaaat cgtctgtttg ttcttttgtt cctaaaaaca
    17161 taccttggaa catccgtaac tttccactag tcgcttcttc aagagaaatt tcagtcaggt
    17221 ctgaaaatac tagtttagca tcattttcaa taacttgatt gggtttgcca ccgagaacaa
    17281 aataatgtga ctcgtcaaaa agtgtcaact gaacatcact cttttcagca aggtcataga
    17341 ggtattcgat gtcagctgga ctaagttctt gccagtcaac caagccccag tcgctagtct
    17401 ggtgagttga acaaccgttg ttaacaatca catactcatt ctggaggtcg agtcccagtt
    17461 ttttgtagta ggggaggaca ccgaaaaggg ggcgacccgt acagagaacc agtttgacac
    17521 ctttttcaat ggcttggtga atagcagcga tgtgagcctg tgggatttcc ttggcttcat
    17581 tgaggagggt tccatccata tccaaggcta gtagtttaat cataggtctt ccttctttat
    17641 cttttgctat tattatagca tattttggag aagaaattga tagaaagctt gagactaatt
    17701 gattttatag tttaagatgt tttgatgaca attcatgatt tgaagaggat atttggcaaa
    17761 gatatgctat actatgtttg tcaatgttgc aactagacaa attaaaaaac caacttaata
    17821 taatagtttt tttgtaagta ggtatgagta tcagattact caactaatct gaagaataat
    17881 ggaggaaata tatcatgatt ttaatgacaa aaaatataaa tctaacaaat gaagaattag
    17941 agctgataca aggtggagca gatccatatg gtaaaaatcc taatggtagg tacgattggg
    18001 aaatagaacc agtattaact ctgccggttc atggattttg tcccagaggc acctatgatt
    18061 taggatatat tggaggaggc aatcatcttt gcaaaggaag tgctgcgaga ttttaagtaa
    18121 aatttattag taatatgaag aaacaagtgg agaaagcaga gaatttaata tgaaaaaacg
    18181 ggctattcaa attttactag cattgtcctt aattttttac aaatcaactt ggttttggag
    18241 agttttcaat cacctcgcaa agccctatct accagcaagt cgtgaatttt ttcagattct
    18301 gcttttgatg gagagcggag ttcttttctt agcggtcatc tatctactgg tttttgcagg
    18361 aaagaaaatt tttcatttca agtggcagct gaggtacttc atctaccttt tactgggcta
    18421 catcatttca tatatgtctg acttcctctt ttcgtatttc atatccctgt cttcaaatca
    18481 gatttctttg aatgaaacgg tagaaatgat ggggagacag gagttccctt atttcttgct
    18541 catcgtttgc ttcatcgccc ctattgctga ggaattgatt tatcgaggtg tgcttatgac
    18601 aacctttttc aaaaactcac cttggtacgg agatgttttg ctttctgcta ttattttcgg
    18661 ttatattcat atcaattttg ctttaactcc tcttgctttt ttcatttatg ctagtggagg
    18721 tcttatttta gctctattgt atcgcatgac taaaaatctc tactatccaa tactagttca
    18781 tattctcatc aatatcactg ccttctggga tgtttggtta ctcctatttt caggaagtta
    18841 gcttactaaa aaaatgtcgg aattttccgg cattttcttt tttcacaaat agtcaacgtt
    18901 tttctttccg atactgaagt ggtgtgtagc cacttatttt tttgaattga ttttgaaaat
    18961 aagattggcg tgagaaaggc agatagtgaa gatagttaag aagaatagga tgttcttttt
    19021 tcctttttgg aaaacttcta aaatatggta taatgaaaag ataaagaagt tgggggtaga
    19081 agatgaacat tcaacaatta cgctatgttg tggctattgc caatagtggt acttttcgtg
    19141 aagctgctga aaagatgtat gttagtcagc cgagtctgtc tatttctgtt cgtgatttgg
    19201 aaaaagagtt gggctttaag attttccgtc ggaccagctc agggactttc ttgacccgtc
    19261 gtgggatgga attttatgaa aaagcgcaag aattggttaa aggatttgat atttttcaaa
    19321 atcagtatgc caatcctgaa gaagaaaaag atgaattttc cgttgctagc cagcactatg
    19381 acttcttacc accaactatt acggcctttt cagagcgcta tcctgactat aagaacttcc
    19441 gtatttttga atcaactact gttcaaatat tagatgaagt ggcgcaaggg catagtgaga
    19501 ttgggattat ctacctcaac aatcaaaata aaaaggggat tatgcaacgg gttgaaaagt
    19561 taggtctgga ggtcatcgaa ttgattcctt tccataccca tatttatctc tgtgagggtc
    19621 atcctttagc tcagaaagag gaattagtca tggaggattt agcggattta ccaacggttc
    19681 gtttcactca agagaaagac gagtaccttt attattcaga gaactttgtc gataccagcg
    19741 ctagctcaca gatgtttaat gtgacagacc gtgccacctt gaatggtatt ttggagcgga
    19801 cggacgccta tgcgacaggt tctggatttt tagatagtga cagtgttaat ggcattacag
    19861 ttattcgtct caaggataac ctagataacc gcatggtcta tgttaaacgt gaagaagtgg
    19921 agcttagtca agctgggact ctcttcgtag aagtcatgca agaatatttt gatcaaaaga
    19981 ggaaatcatg aaaaaaagag caatagtggc agtcattgta ctgcttttaa ttgggctgga
    20041 tcagttggtc aaatcctata tcgtccagca gattccactg ggtgaagtgc gctcctggat
    20101 tcccaatttc gttagcttga cctacctgca aaatcgaggt gcagcctttt ctatcttaca
    20161 agatcagcag ctgttattcg ctgtcattac tctggttgtc gtgataggtg ccatttggta
    20221 tttacataaa cacatggagg actcattctg gatggtcttg ggtttgactc taataatcgc
    20281 gggtggtctt ggaaacttta ttgacagggt cagtcagggc tttgttgtgg atatgtttca
    20341 ccttgacttt atcaactttg caattttcaa tgtggcagat agctatctga cggttggagt
    20401 gattatttta ttgattgcaa tgctaaaaga ggaaataaat ggaaattaaa attgaaactg
    20461 gtggtctgcg tttggataag gctttgtcag atttgtcaga attatcacgt agtctcgcga
    20521 atgaacaaat taaatcaggc caggtcttgg tcaatggtca agtcaagaaa gctaaataca
    20581 cagtccaaga gggtgatgtc gtcacttacc atgtgccaga accagaggta ttagagtatg
    20641 tggctgagga tcttccgcta gaaatagtct accaagatga ggatgtggct gtcgttaaca
    20701 aacctcaggg aatggttgtg cacccgagtg ctggtcatac cagtggaacc ctagtaaatg
    20761 ccctcatgta tcatattaag gacttgtcgg gtatcaatgg ggttctgcgt ccagggattg
    20821 ttcaccgtat tgataaggat acgtcaggtc ttctcatgat tgctaaaaac gatgatgcgc
    20881 atctagcact tgcccaagaa ctcaaggata aaaagtctct ccgcaaatat tgggcgattg
    20941 ttcatggaaa tctgcctaat gatcgtggtg taattgaagc gccgattggc cggagtgaaa
    21001 aagaccgtaa gaaacaggct gtaactgcta aagggaagcc tgcagtgacg cgttttcacg
    21061 tcttggaacg ctttggcgat tatagcttag tagagttgca actggagaca gggcgcactc
    21121 atcaaatccg tgtccacatg gcttatatcg gccattcagt cgctggtgat gaggtctatg
    21181 gtcctcgcaa gactttgaaa ggacatggac aatttcttca tgccaagact ttaggtttta
    21241 ctcatccgag aacaggtaag accttggaat ttaaagcaga tatcccagag atttttaagg
    21301 aaaccttgga gagattgaga aagtaagaat gaaaaagaaa ttaactagtt tagcacttgt
    21361 aggcgctttt ttaggtttgt catggtatgg gaatgttcag gctcaagaaa gttcaggaaa
    21421 taaaatccac tttatcaatg ttcaagaagg tggcagtgat gcgattattc ttgaaagcaa
    21481 tggacatttt gccatggtgg atacaggaga agattatgat ttcccagatg gaagtgattc
    21541 tcgctatcca tggagagaag gaattgaaac gtcttataag catgttctaa cagaccgtgt
    21601 ctttcgtcgt ttgaaggaat tgggtgtcca aaaacttgat tttattttgg tgacccatac
    21661 ccacagtgat catattggaa atgttgatga attactgtct acctatccag ttgaccgagt
    21721 ctatcttaaa aaatatagtg ataatcgtat tactaattct gaacgtctat gggataatct
    21781 gtacggttat gataaggttt tacagactgc ttcagaaaaa ggtgtttcag ttattcaaaa
    21841 tatcacacaa ggggatgctc attttcagtt tggggacatg gatattgagc tctataacta
    21901 tgaaaatgaa accgattcat cgggtgaatt aaagaaaatt tgggatgata attccaattc
    21961 cttgattagt gtggtaaaag tcaatggcaa gaaaatttac cttgggggcg acttagataa
    22021 tgttcatgga gcagaagaca agtatggtcc tctcattgga aaagttgatt tgatgaagtt
    22081 taatcatcat ttagatacaa aaaattctaa tacaaaaaat tttataaaaa atttgtctcc
    22141 tgagataatt gttcaaactt cctcctcacc aattggttcg tatgatagcg gtcaagaaat
    22201 aaatagggaa tatattagtt ggcttgaatc aatgggaatt caacatataa atgcagccag
    22261 caaagactat gatgcaacag tttttgatat tcgacaagac ggtttagtaa acatttcaac
    22321 ttcctacaag ccgattccaa gttttcaagc tggttggcat aagagttcat atggaaactg
    22381 gtggtatcaa gcgcctgatt ctacaggaga atatgctgtc ggttggaatg aaattaaggg
    22441 tgaatggtat tactttaacc aaacgggtat cttattacag aatcaatgga aaaaatggaa
    22501 caatcgttgg ttctatttga cagactctgg tgttgctgct aaaaattgga agaaaatcgc
    22561 tggaatctgg tattatttta acaaagaaaa ccagatggaa attggttgga ttcaagataa
    22621 agagcagtgg tattatttgg atgttgatgg ctctatgaag acaggttggc ttcaatataa
    22681 ggggcaatgg tattactttg ctccatcagg ggaaatgaaa acgggctggg taaaagataa
    22741 agaagcctgg tactatatgg attctactgg tatcatgaag acaggtgaga tagaagttgc
    22801 tggacatcat tactatctag aagaatcagg agctatgaag caaggctggc ttaaaaaggc
    22861 aaatgattgg tatttctaca agacagatgg ttcacgagct ataggttgga ttaaagacaa
    22921 ggataaatgg tacttcttga aagaaaatgg tcaattactt gtgaatggta agacaccaga
    22981 aggctatact gtggattcaa gtggtgcctg gttagtggat gttccgatcg agaaatctgc
    23041 tacaattaaa actacaagtc attcagaaat aaaagaatcc aaagaagtag tgaaaaagga
    23101 tcttgaaaat aaagaaacga gtcaacatga aagtgttaca aattcttcaa ctagtcaaga
    23161 tttgacatcc tcaacttcac aaagctctga aacgagtgta aacaaatcgg aatcagaaca
    23221 gtagtagaaa agaaggtttt agggccttct ttttcctatc aactcttttc tatttcctgt
    23281 tattcatgtt ataatggata aatatgaata atcggagtga gactatgaaa tacaaacgga
    23341 ttgtctttaa ggtgggtact tcttctctga caaatgagga tggaagttta tcacgtagta
    23401 aggtaaagga tattacccag cagttggcta tgctgcacga ggctggtcat gagttgattt
    23461 tggtgtcttc aggtgccatt gcggctggtt ttggagcctt aggatttaaa aagcgtccga
    23521 ctaaaattgc tgataaacag gcttcagcag cggtagggca ggggcttttg ttggaagaat
    23581 atacaaccaa tcttctcttg cgtcaaatcg tttctgcaca aatcttgctg acccaagatg
    23641 actttgtgga taagcgtcgt tataaaaatg cccatcaggc tttgtcggtt ttgctcaacc
    23701 gtggggcaat tcctatcatc aatgagaatg atagtgtcgt tattgatgag gtcaaggttg
    23761 gggacaatga cactctaagt gcccaagtag cggcgatggt ccaagcagac cttttggttc
    23821 tcttgacaga tgtggacggt ctctatactg gaaatcctaa ttcagatcca agagccaaac
    23881 gcttggagag aatcgagacc atcaatcgtg agattattga tatggctggt ggagctggtt
    23941 cgtcaaacgg aactgggggt atgttaacca aaatcaaggc tgcaactatc gcgacggaat
    24001 caggagttcc tgtttatatc tgctcatcct tgaaatcaga ttccatgatt gaggcggcag
    24061 aggagaccga ggatggttct tactttgttg ctcaagagaa ggggcttcgt acccagaaac
    24121 aatggcttgc cttctatgct cagagtcaag gttctatttg ggttgataaa ggggctgcgg
    24181 aagctctctc tcaacatgga aagagtcttc tcttatctgg tatcgttgaa gcagaaggag
    24241 tcttttctta cggtgatatc gtgacagtat ttgacaagga aagtggaaaa tcacttggaa
    24301 aaggacgcgt gcaatttgga gcatctgctt tggaggatat gttgcgttct caaaaagcca
    24361 agggtgtctt gatttaccgt gacgactgga tttccattac tcctgaaatc caactacttt
    24421 ttacagaatt ttagaggtaa actatggtga gtagacaaga acaatttgaa caggtacagg
    24481 ctgttaaaaa atcgattaac acagctagtg aagaagtgaa aaaccaagcc ttgctagcca
    24541 tggctgatca cttagtggct gctactgagg aaattttagc ggctaatgcc ctcgatatgg
    24601 cagcggctaa ggggaaaatc tcagatgtga tgttggatcg tctttatttg gatgcagatc
    24661 gtatagaagc gatggcaaga ggaattcgtg aagtggttgc cttaccagat ccaatcggtg
    24721 aagttttaga aacaagtcag cttgaaaatg gtttggttat cacaaaaaaa cgtgtagcta
    24781 tgggtgtcat cggtattatc tatgaaagcc gtccaaatgt gacgtctgat gcggctgctt
    24841 tgactcttaa gagtggaaat gcggttgttc ttcgtagtgg taaggatgcc tatcaaacaa
    24901 cccatgccat tgtcacagcc ttgaagaagg gcttggagac gactactatt catccaaatg
    24961 tgattcaact ggtggaggat actagccgtg aaagtagcta tgctatgatg aaggccaagg
    25021 gctatctaga ccttctcatt cctcgtggag gagctggctt gatcaatgca gtggttgaga
    25081 atgcgattgt acctgttatc gagacaggga ctgggattgt ccatgtctat gtggataagg
    25141 atgcagacga agacaaggcg ctgtctatca ttaacaatgc taaaaccagt cgtccttctg
    25201 tttgtaatgc catggaggtt ctgctggttc atgaagacaa ggcagcaagc ttccttcctc
    25261 gcttggagca agtgttggtt gcagatcgaa aagaagctgg gttggaacca attcaattcc
    25321 gcctagatag caaagcaagc cagtttgttt caggtcaagc tgctcaagca caagactttg
    25381 ataccgagtt tttagactat attctagctg ttaaggttgt gagcagttta gaagaagcga
    25441 ttgcgcatat tgaatcccac agtacccatc attcggatgc cattgtgacg gaaaatgctg
    25501 aagctgcagc atactttaca gatcaagtgg actctgcagc ggtgtatgtt aatgcctcaa
    25561 ctcgtttcac agatggagga caatttggtc ttggttgtga aatggggatt tctactcaga
    25621 aattgcacgc gcgtggtccc atgggcttga aggaattgac tagctacaag tatgtggtca
    25681 ctggtgatgg acagataagg gagtaagaga tgaagattgg atttatcggt ttggggaata
    25741 tgggtgctag tttagccaag tctgtcttgc aggctagacc gtcagatgag attctccttg
    25801 ccaatcgtag tcaagtcaag gtggatgctt tcatcgctaa cttcggtggt cagacttcta
    25861 acaatgaaga aatattcgaa gaagcagatg tgatttttct aggtgttaag cctgctcagt
    25921 tttctgaact gctttctcaa taccagacca tccttgaaaa aagagaaagt cttcttttga
    25981 tttcgatggc agctggattg accttagaaa aactagcaag tcttatccca agccaacacc
    26041 gaattattcg tatgatgcct aatacccctg cttctatcgg gcaaggagtg attagttatg
    26101 ccttgtctcc taattgcagg gctgaggaca gtgagatctt ttgtcagctt ttagccaagg
    26161 ctggtctctt ggttgaatta ggagaaagct taatcaatgc agcgacaggt cttgcaggtt
    26221 gtggaccagc ctttgtctat ctctttattg aggccttggc agatgcaggt gttcagacgg
    26281 gattaccacg agaaacagcc ttgaaaatgg cagcccaaac tgtggtagga gctgggcaat
    26341 tggtcctaga aagccagcaa catcctggag ttttgaaaga tcaagtttgt agtccaggcg
    26401 gttcgactat tgctggcgta gcaagcttag aagcgcatgc ttttcgaggc acagtcatgg
    26461 aggcagttca tcaagcctat aaacgaacac aagaactagg taaataagag gtagctttga
    26521 ctgcctcttt tatggtggtt gatactcttc gaaaatcaaa ttcaaactgc gtcagcttca
    26581 ccttgccgta ctcaagtaca gcctgcggct agcttcctag tttgctcttt gattttcatt
    26641 gagtatgaaa tgagaagaca aaaagattgt cacaaacccc tatttttttg atagaataga
    26701 agtagtaaaa aagaaatgag ttagacatgt caaaaggatt tttagtctct cttgagggac
    26761 cagagggagc aggcaagacc agtgttttag aggctctgct accaatttta gaggaaaaag
    26821 gagtagaggt gttgacgacc cgtgaacctg gcggagtctt gattggggag aagattcggg
    26881 aagtgatttt ggatccaagt catactcaga tggatgctaa aacagagcta cttctctata
    26941 ttgccagtcg cagacagcat ttggtggaaa aagttcttcc agcccttgaa gctggcaagt
    27001 tggtcatcat ggatcgtttt atcgatagtt ctgttgccta tcagggattt ggtcgtggct
    27061 tagatattga agccattgac tggctcaatc agtttgcgac agatggcctc aaacccgatt
    27121 tgacactcta ttttgacatc gaggtggaag aagggctggc tcgtattgct gctaatagtg
    27181 accgcgaggt taatcgtttg gatttggaag ggttggactt gcataaaaaa gttcgtcaag
    27241 gctacctttc tcttctggat aaagagggaa atcgcattgt caagattgat gctagtctcc
    27301 ctttggagca agttgtggaa actaccaagg ctgtcttgtt tgacggaatg ggcttggcca
    27361 aatgaaacaa gatcaactaa aggcttggca accagctcag tttgaccgtt ttgtccgtat
    27421 cttagaacaa gaccagctca atcacgccta tctcttttca ggtttctttg gaagcttgga
    27481 aatagcgcaa tttttagcta agagcctctt ttgtacggat aaagttggcg tcttaccatg
    27541 tgagaaatgc cgaagttgca agctgattga acagggagaa tttcccgatg tcaccttgat
    27601 taagccagtc aatcaggtca tcaagacaga acgcattagg gaattggtgg gacagttttc
    27661 tcaagcaggg attgaaagcc agcaacaggt ctttattatc gagcaagcgg ataaaatgca
    27721 tcccaacgca gccaattctc tgctcaaggt catcgaagaa ccccagagtg aagtttatat
    27781 tttcttcttg actagcgatg aggaaaagat gttaccgaca atccgaagtc ggactcagat
    27841 cttccacttt aaaaagcaag aagaaaaact tatcttactc ttagaacaaa tgggacttgt
    27901 taagaaaaaa gcgactcttt tagctaagtt tagtcaatcg cgagctgaag cagaaaagtt
    27961 ggctaatcag gcaagttttt ggaccttggt cgatgaaagt gaacgcctgc tgacttggtt
    28021 agtagctaag aaaaaagaaa gttatctaca ggttgccaaa ttagccaact tggcagatga
    28081 taaggaaaaa caggatcagg ttttacggat tcttgaagtt ctctgcgggc aggacctctt
    28141 gcaggtaaga gtaagagtga ttctacaaga tttactagaa gctagaaaaa tgtggcaagc
    28201 taatgtcagc tttcaaaatg ccatggaata tctggtcttg aaagaaatat aaactcaaaa
    28261 atgaatgata aagaaaggaa agggctgttt tatggacaaa aaagaattat ttgacgcgct
    28321 ggatgatttt tcccaacaat tattggtaac cttagccgat gtggaagcca tcaagaaaaa
    28381 tctcaagagc ttggtagaag aaaatacagc tcttcgcttg gaaaatagta agttgcgaga
    28441 acgcttgggt gaggtggaag cagacgctcc cgtcaaggcc aagcatgttc gcgaaagtgt
    28501 ccgtcgtatt taccgtgatg gatttcacgt atgtaatgat ttttatggac aacgtcgaga
    28561 gcaggacgaa gaatgtatgt tctgtgatga gttgttatac agggagtaag catgcaaatt
    28621 caaaaaagtt ttaaggggca gtctccctat ggcaagctgt atctagtggc aacgccgatt
    28681 ggcaatctag atgatatgac ttttcgtgct atccagatct tgaaagaagt ggactggatt
    28741 gctgctgagg atacgcgcaa tacaggtctt ttgctcaagc attttgacat ttccaccaag
    28801 cagatcagtt ttcatgagca caatgccaag gaaaaaattc ctgatttgat tggtttcttg
    28861 aaagcagggc aaagtattgc tcaggtctct gatgccggtt tgcctagcat ttcagaccct
    28921 ggtcatgatt tggttaaggc agctattgag gaagaaattg cagttgtgac agttccaggt
    28981 gcctctgcag gaatttctgc cttgattgcc agtggtttag cgccgcagcc acatatcttt
    29041 tacggctttt tgccgagaaa atcaggccag caaaagcaat ttttcgactc gaaaaaagac
    29101 tatcctgaaa ctcagatttt ttatgaatca cctcatcgtg tagcagacac gttggaaaat
    29161 atgttagaag tctacggtga ccgctccgtt gtcttggtca gggaattgac caaaatctat
    29221 gaagaatacc aacgaggtac tatctctgag ttattagaaa gcattgctga aacgccactc
    29281 aagggcgaat gtcttctcat tgttgagggt gccagtcagg gtgtggagga aaaggacgag
    29341 gaagacttgt tcgtagaaat tcaaacccgc atccagcaag gtgtgaagaa aaaccaagct
    29401 atcaaggaag tcgctaagat ttaccagtgg aataaaagtc agctctacgc tgcctaccac
    29461 gactgggaag aaaaacaata aagggagaca ggatgtaata attctgtctg tttctgttta
    29521 acttaattag tgatgataat ataaagatgt atcacttggt atagaagctt tggtattaag
    29581 ttttttatta agcccatacg gaataccgat ggttggagca gcagttatag cgttcttaga
    29641 aggtataaat agaaaaataa ggtcatttta aatcaaagga ttgataaatc agaaagaagg
    29701 tgattttttg cgaacatacg aaaataaaga agaactaaaa gctgagatag agaaaacatt
    29761 tgagaaatat attttagaat ttgataatat tccagaaaat ttaaaagata agagagctga
    29821 tgaagttgac agaactccag cagaaaacct tgcttatcag gttggttgga ccaacttggt
    29881 tcttaaatgg gaagaagatg aaagaaaggg gcttcaagta aaaacaccat cggataaatt
    29941 taaatggaat caacttggtg aattatatca gtggttcaca gatacctacg ctcatttatc
    30001 tctgcaagag ttgaaagcaa aattaaatga aaatattaat tctatctctg caatgattga
    30061 ttcgttgagt gaggaagaat tatttgaacc gcatatgaga aagtgggctg atgaagcgac
    30121 taaaacagcg acttgggaag tgtataagtt tattcatgta aatacggttg caccttttgg
    30181 aactttcaga actaaaatca gaaaatggaa gaagatagta ttataaatta tatttttaac
    30241 tttaaaaaat ttcataaaaa tggttaccaa aggcgataga agaaaaacta tcgtcttttt
    30301 ctttgcaaat ttttaagaag ggaggtgatc ttgcatggac tttgaatatt tttataacag
    30361 agaagcggaa agatttaact tcttaaaagt accggagata ttagttgata gagaagaatt
    30421 tcggggctta tcagcagaag caattatcct ttattccata cttcttaaac agacaggaat
    30481 gtcatttaag aataactgga tagacaagga aggcagagta tttatctatt ttactgtcga
    30541 agaaattatg aaaagaagaa atatctcaaa gccaactgcc ataaaaacat tagatgagct
    30601 tgatgtaaaa aaaggaatag gactgatcga aagagtaagg cttggacttg gtaagccgaa
    30661 catcatttat gttaaagact ttatgagtat atttcaggta aaagaaaatg acttacagaa
    30721 gtcaaaaaac ttaacttcag aagtaaaaga ttttaacctc agaagtaaag aaaatgaact
    30781 tcaagaggtt aagaaccttg actctaacta tatagagaat aataagagta agtatagtaa
    30841 gagagaatat agttttggtg aaaacggact tggaacattt caaaatgtgt ttttagctgc
    30901 tgaagatata tcggatttac aaatcataat gaactcacag cttgagaatt acattagact
    30961 tcctgcaaaa ctagaatcct agttcatgat tgataatacc agcaatcaaa ttcattcgta
    31021 atccgaagcg tttacgatga tttcgatagg ttgttgaaaa cattttaaac gtttttactt
    31081 tggcaaagat gttctcaacc ttgcttctct ccttagatag cgcatggtta caggctttat
    31141 cttcaactgt tagcggtttg agtttgctgg atttacgtgg agtttgtgct tgaggatata
    31201 tcttcgtgag tccttgataa ccactgtcag ccaagatttt accagcttgt ccgatatttc
    31261 tgcgactcat tttgaacaac ttcatatcat aacaatagtt cacagtgata tccaaagaaa
    31321 caattctccc ttgacttgtg acaatcgttt gagccttcat agcgtggcat ttctttttac
    31381 cataataatt cgctaattgt ttttttaggg cgattgattt ttacttccgt cgcatcaatc
    31441 attaccgtgt cctcagaact gagaggagtt cttgaaatcg taaaaccact ttgaacaaga
    31501 gttacttcaa cccattggct ccgatggatt aagttgcttt cgtgaatacc aaaatcagcc
    31561 gcaatttctt cataagtgcg gtattctcgc atatattgaa gagtggtcat aagaaggtct
    31621 tctaggctta atttaggttt tcgtccacct tttgcatgtt taagttgata agctgttttt
    31681 aatacagtta acatctcttc aaaagtcgtg cgctgaacac caacaagacg cttaaatcgt
    31741 gcatcagtta gttgtttact tgcttcatca ttcatagaac tactatacca tattttgttt
    31801 cgcaggaagt ctattggaaa gtaagaaata ttgaagctga ggctattaga agaaattgtg
    31861 agcgtggtgc tattttttca ggtaaaataa aatatcacga agattcacag tttaaaggag
    31921 atcactatgt tgaatgttat gctgttttag ataatacggt tatagcaaga gatagaataa
    31981 cagtccctat cgatccgtta tgtggaaaag attttataga gtagcatata attgattctt
    32041 aactggaata ctcactatct ctttacatca agaaaatgac taaacaggga agtttgcctt
    32101 cttccctttt tttgttatac tagtagaaga aaaaatagaa agatttgtgg gagtgaaaaa
    32161 gtcctgtgga ctttttcagc ctgagccaag aaactcgaaa gctcgtaagt ctgattggct
    32221 ttttcaatgt gaatcttaac ttcatactcc caaagaggta ttagtgtcgt gtctcaatct
    32281 tatatcaatg ttatcggtgc tggtttggca ggttctgaag cagcctacca aatcgcagag
    32341 cgtggtattc cagttaaact ttatgaaatg cgtggtgtca agtcaacacc tcagcacaaa
    32401 acagacaatt ttgctgagtt ggtttgttcc aattccttgc gtggagatgc tttgacaaat
    32461 gctgttggcc tccttaagga agaaatgcgt cgcttgggtt ctgtgatttt ggaatctgct
    32521 gaggctacac gtgttcctgc aggtggtgcc cttgcagtgg accgtgatgg tttctctcaa
    32581 atggtgaccg aaaaagttgc caaccacccc ttgattgaag tggttcgtga tgaaattaca
    32641 gaattgccga cagatgttat tacggttatc gctactggtc ctttgacaag tgatgccttg
    32701 gctgaaaaga ttcatgccct taatgacggt gatggttttt atttctacga tgcggcagcg
    32761 cctattatcg atgtcaacac tatcgatatg agcaaggtct acctcaaatc acgttatgat
    32821 aagggagaag cggcctacct caatgcccct atgaccaagc aagaatttat ggatttccat
    32881 gaagctttgg tcaatgcaga agaagcaccg cttaattctt ttgaaaaaga aaagtacttt
    32941 gaaggatgta tgcctatcga agtcatggcc aaacgtggca ttaaaaccat gctttatggt
    33001 cctatgaaac cagttggtct tgagtatccg gacgactaca caggacctcg tgatggagaa
    33061 tttaaaacac cttatgcggt tgtccagctc cgtcaggata atgcagctgg tagcctatac
    33121 aatattgttg gtttccagac ccacctcaaa tggggagaac aaaagcgtgt cttccaaatg
    33181 attccgggtc ttgaaaatgc ggagtttgtc cgttatggtg tcatgcatcg caattcttac
    33241 atggattcac caaatcttct tgagcagact taccgttcca agaaacaacc aaatctcttc
    33301 tttgctggtc agatgacggg ggtggaaggc tatgttgagt cggcggcttc gggcttagtt
    33361 gcgggaatta acgcagctcg tctcttcaag gaagaaagcg aggttatttt ccccgagacg
    33421 acagcgattg gaagcttagc tcattacatt acccatgccg acagcaaaca tttccaacca
    33481 atgaatgtca attttgggat catcaaggag ttggaaggcg agcgtatccg tgataagaag
    33541 gctcgttatg aaaaaattgc agagcgtgcc cttgccgact tagaggaatt tttgactgtc
    33601 taattttttt gaaagaattg ctcatgatac tataaaaatc ttagaaattg tgataaaata
    33661 ggtaggatga aagaaggaga gtgaaaatgg cgaatcccaa gtataaacgt attttaatca
    33721 agttatcagg tgaagccctt gccggtgaac gtggcgtagg gattgatatc caaacagttc
    33781 aaacaatcgc aaaagagatt caagaagttc atagcttagg tatcgaaatt gcccttgtta
    33841 ttggtggagg aaatctttgg cgtggagaac ctgcagcaga agcaggtatg gaccgtgttc
    33901 aggcagatta cactggaatg cttgggactg ttatgaatgc tcttgtgatg gcagattcat
    33961 tgcaacaagt tggggttgat acgcgtgtac aaacagctat tgctatgcaa caagtggcag
    34021 agccttatgt ccgtggacgt gcccttcgtc accttgaaaa aggccgtatc gttatctttg
    34081 gtgctggaat tggttcacca tacttctcga cagatacaac agcggccctt cgtgcagctg
    34141 aaatcgaagc agatgccatc ctcatggcta aaaatggcgt cgatggtgtg tacaatgccg
    34201 atcctaagaa ggacaagaca gccgttaagt ttgaagaatt gacccaccgt gatgttatca
    34261 acaaaggtct tcgtatcatg gactcaacag cctcaaccct ctcaatggac aacgacattg
    34321 acttggttgt cttcaacatg aaccaatcag gcaacatcaa acgtgtcgta tttggtgaaa
    34381 atatcggaac aacagtttca aataatatcg aagaaaagga ataagaaaga atatggctaa
    34441 cgtaattatt gaaaaagcta aagagagaat gacccaatct caccaatcac ttgctcgtga
    34501 atttggtggt atccgtgctg gtcgtgccaa tgcaagcttg cttgaccgtg tacatgtaga
    34561 atactatgga gtcgaaactc ctcttaacca aatcgcttca attacgattc cagaagcgcg
    34621 tgttttgttg gtaacaccat ttgacaagtc ttcattgaaa gacatcgaac gtgccttgaa
    34681 cgcttctgaa cttggtatca caccggctaa tgacggttct gtgattcgct tggttatccc
    34741 agctcttaca gaagaaactc gtcgtgacct tgctaaagaa gtgaagaagg tcggcgaaaa
    34801 tgctaaagtg gctgtccgca atatccgtcg cgatgctatg gacgaagcta agaaacaaga
    34861 aaaagcaaaa gaaatcactg aagacgaatt gaagactctt gaaaaagata ttcaaaaagt
    34921 aacagacgat gctgttaaac acatcgacga catgactgct aacaaagaga aagaactttt
    34981 ggaagtctaa aaataaacag aaaaactcag ttggcattgc tggctgagtt ttattcgaaa
    35041 gaaggaaata tgaatacaaa tcttgcaagt tttatcgttg gactgatcat cgatgaaaat
    35101 gaccgttttt actttgtgca aaaggatggt caaacctatg ctcttgctaa ggaagaaggt
    35161 caacatacag taggggatac ggtcaaaggt tttgcataca cggatatgaa gcaaaaactc
    35221 cgcctgacaa ccttagaagt gactgccact caggaccaat ttggttgggg acgtgtcaca
    35281 gaggttcgta aggatttggg tgtctttgtg gatacaggcc ttcctgacaa ggaaatcgtt
    35341 gtgtcactcg atattctccc tgagctcaag gaactctggc ctaagaaggg cgaccaactc
    35401 tacatccgtc ttgaagtgga taagaaagac cgtatctggg gcctcttggc ttatcaagaa
    35461 gacttccaac gtcttgctcg tcctgcctac aacaacatgc agaaccaaaa ctggccagcc
    35521 attgtttacc gtctcaagct gtcaggaact tttgtttacc taccagaaaa taatatgctt
    35581 ggttttattc atcctagcga gcgttacgca gagccacgtt tggggcaagt attagatgcg
    35641 cgcgttattg gtttccgtga agtggaccgc actctgaacc tctccctcaa accacgctcc
    35701 tttgaaatgt tggaaaacga tgctcagatg attttgactt atttggaaag taatggcggt
    35761 ttcatgacct taaatgataa gtcatctcca gacgacatca aggcaacctt tggcatttct
    35821 aaaggtcagt tcaagaaagc tttaggtggt cttatgaagg ctggtaaaat caagcaggac
    35881 cagtttggga cagagttgat ttagggaggc ttatgagaaa atcattttac acttggctca
    35941 tgaccgagcg caatcctaaa agtaacagtc ccaaagcaat tttggcagac ctcgcttttg
    36001 aagaggcagc ttttccaaaa catacagatg attttgatga ggtcagtcgc tttttggagg
    36061 agcatgccag tttctctttt aacctaggag attttgacag catttggcag gaatatctag
    36121 aacactagca tttattcatt gggtttgggc tagtaatttc tccatccctc tgctataata
    36181 aaaagaaata aaaggattag agaggttctt tatttgaagg aacattcaat agacattcaa
    36241 ctgagtcatc cagatgacct gtttcatctt tttggttcca atgaacgcca tcttcgtttg
    36301 atggaagaag agcttgatgt tgtgattcat gctcgtacgg agattgtcca ggttttggga
    36361 gaagagtctg cctgtgagga agcccgtcaa gttattcagg ctttgatggt cttggtaaat
    36421 cgtgggatga ccgttggtac gccagatgta gtcactgcga ttagcatggt caaaaatgat
    36481 gaaattgaca agtttgtcgc cctttacgaa gaagaaatta tcaaagataa tactgggaaa
    36541 cctatccgtg tcaaaacact agggcaaaag ctttatgtgg acagtgtcaa acagcatgat
    36601 gtgacctttg gaattgggcc agcaggtaca gggaagacct tccttgcagt gaccttggca
    36661 gtgactgccc ttaaacgtgg gcaagtcaag cgaattatcc taactcgtcc agcggtggaa
    36721 gcgggagaga gtcttggatt tcttccgggt gatcttaagg agaaggtgga tccttacctt
    36781 cgtcctgttt acgatgcctt gtatcaaatt cttgggaaag accaaacgac tcgtctcatg
    36841 gagcgtgaaa ttatcgaaat tgcgcccctt gcctatatgc gtggccggac cttggatgat
    36901 gcctttgtca ttctcgatga ggcgcaaaac acgaccatca tgcagatgaa gatgttcttg
    36961 acgcgtttag gctttcattc taagatgatt gtcaatggag atatcagtca gattgacctg
    37021 ccacgtaatg tcaagtccgg tttgattgat gctcaagaga aactcaagaa catccatcag
    37081 attgactttg ttcatttttc agccaaggat gtggttcgcc atccagttgt cgctcagatt
    37141 atccgagcct atgaatattc tactgaagtt gcacacgact gattttgagg aagttcgcct
    37201 gcaaaagaat atacttttga gagaggaaaa tccagttgta taggctaaag gttttatcca
    37261 aaggtctgag acaacgatta ggcacgatgg aaagaacttt tatgtggctg atgacgatca
    37321 gtgcatcttc ctgtgtcata atcacagggc acaagaaagt aggaatttga aaagatgatt
    37381 gaccaactat ctaagtatta cagttgtagg atactaactg aaaaggatat tccaagtatt
    37441 ttatctttat atgaaagtaa tcctctgtat tttcagcatt gtccaccaga accaaatttt
    37501 gcaactgtaa aagaggacat gctttgtcta cctgaaggta aagctaaggc tgataagttt
    37561 tttgttggat tttggaatgg atctgacctt gtggctgtta tggattttgt ctatgcatat
    37621 cctgatgagg agactgtttt tattggtttg tttatggttg atcaagccta tcagaggaaa
    37681 gggattggta gtcatattgt gacagaagca ctagcttatt ttgctaagaa ctttcgaaag
    37741 gcacgtttgg cttatgttaa gggaaatccg caatctcagc atttttggga aaagcagggc
    37801 tttaaatcaa ttggatgcga ggttaagcaa gaactctata cggttgttat cgctgaacag
    37861 agcctagaag attagaaatg gcatcaagta agaactattt ggaatttgtt ttggaacaat
    37921 tatcaggatt agatgatgtg acttaccgtt ccatgatggg ggagtatatt ctttacttcc
    37981 gcggcaagat tattggcggc atttatgacg atcgcttttt agttaaaccc gtgcaagcag
    38041 tcttagataa gattgaccaa tcttcttttg agtttccata caaaggtgcc aaagaaatga
    38101 tttgagtgga agaacttgat aataagatgt ttctataaga cctgatttta gctatgtata
    38161 accaactgcc aacgcccaaa cctaaaaaga aaaagcaagg gtgaacgaag taaaaaagaa
    38221 gtctgctaag gccctgtctt tgcacgggta aaattttata tataaaaaga agctgggact
    38281 aaagagctca gcttcctttg gtttatataa ttgtcattac aagacgaagt ggttgggcga
    38341 aactctgttg actttattca atttagagtt tcttatgcac aattgagtct ggaacgaaag
    38401 tctccagttg caaagtatac agtacaataa accaacgatg taatagctga tgacacaaag
    38461 cacagtgggt aggacttgcg aagtcaccct tttcttttta aaatttatac taaatcattg
    38521 atatcagtgt agtcacgatt aagtccttga gcaactggta ggttagtcaa gtaaccttga
    38581 taagtagtca caccttgacg caagccttca tcttcagaga ttgcttgtgc gaatcctttg
    38641 ccagccaaag cttcgatata aggaagagtg acattggtta gggcgatggt tgaagtgcgg
    38701 gcaaccgcac cagggatatt ggcaacggca tagtggagaa caccgtgttt ttcatagacg
    38761 ggttcatcgt gcgttgtcac acggtcagct gtttcgataa ggccaccttg gtcaacagca
    38821 acgtcaacga tacagagcct ggacgcattt gtttgaccat ctcatctgtc accaattccg
    38881 gtgcttttgc accagggatg agaatggctc caatcaccac atcagcatct ctcacacttg
    38941 cttcaatgtt gaatgaatta gacataagag tttgaatttg acttccaaag acttcttcta
    39001 gaactgagag acgcttggca ctaatatcta aaatagtcac ttgagcacca agaccaaggg
    39061 cgatgcgggc agcatgtgta ccgacgacac catcaccgat gatagttact tttccttttg
    39121 gaacacctgg tacaccacca agtagaacac cagagccacc agcttgctta gtaaggaagt
    39181 gagctccgat ttgaacagcc atacgacctg caacctcact cataggaacg aggagcggta
    39241 gttgtccttg attgtcacga acagtttcat aggcaattcc tgttgttttt gctgctaaca
    39301 tagcatctgc taattctgga gcagcggcca tgtgcaagta ggtgaagaga agaagatcgt
    39361 cgcgcaagta accgtattca gaacttaaag gttcttttac tttcacaacc aactctgctg
    39421 cccaagcttc accagcagta gcgacaatct cagctccttg cttttgatag tcagcatcag
    39481 taaagccaga accgagacca gcatttgttt cgataaggac acgatgacca cgactaacta
    39541 agctatgaac acctgcaggt gtgagggcga cacggttttc gttattttta atttcttttg
    39601 ggattccgat taacattgag ataacctacc tttcaattga cggtcttgtt ttggttgtca
    39661 cattccagtt cataaatcaa aaatgtgacg gtttcattgt atatgaaacc gcttcaaaaa
    39721 tcaagaaaaa cttgtcatcc caattttttt atgctagact agtgaaaatc aagctctaat
    39781 ggagggaaaa gtatggaatc aatttttgta aaacttgccc agtatccgtc tatagaaacg
    39841 gagcgtttat tgcttagacc tgtaactttg gatgatgcgg aagcaatgtt tgactatgcc
    39901 tcggacaagg gtaatacacg ttacactttt ccaaccaatc aaagcttgga agaaaccaag
    39961 aataacattg ctcagttcta tttggctaat cccttgggac gttggggaat agaactaaaa
    40021 agcaatggtc agtttattgg aaccattgac ttgcacaaga ttgattctgt tcttaagaag
    40081 gcagctattg gctacattat caataaaaag tattggaatc aaggattaac gacagaagcc
    40141 aatcgtgctg tgattgagct agcttttgag aagataggga tgaataagtt gactgccctt
    40201 cacgataagg acaatcctgc gtcaggaaag gtcatggaga aatcaggcat gcgtttttcc
    40261 catgcagaac catatgcttg tatggaccag catgaaaaag gccgaatcgt gacaagagtt
    40321 cattatgtct tgaccaagga agactatttt gcaaataaat aagcagttga aaagaaattt
    40381 ttcgactgtt ttttcttcct cttacgaata atctaagaga ggagaaaata tggaagcaat
    40441 tatcgagaaa atcaaagagt ataaaatcat cgtcatctgt actggtctgg gcttgcttgt
    40501 aggaggattt ttcctgctaa aaccagctcc acaaacacct gtcaaagaga cgaatttgca
    40561 ggctgaagtt gcagctgttt ccaaggactc atcgaccgaa aaggaagtga agaaggaaga
    40621 aaaggaagaa ccccttgaac aagatctaat cacagtagat gtcaaaggtg ctgtcaaatc
    40681 gccagggatt tatgacttgc ctgtaggtag tcgagtcaat gatgctgttc agaaggctgg
    40741 tggcttgaca gagcaagcag acagcaagtc gctcaatcta gctcagaaag ttagtgatga
    40801 ggctctggtt tacgttccta ctaagggaga agaagcagtt agtcaacaga ctggttcggg
    40861 gacagcttct tcaacaagca aggaaaagaa ggtcaatctc aacaaggcca gtctggaaga
    40921 actcaagcag gtcaaaggac tgggaggaaa acgtgctcag gatattatcg accatcgtga
    40981 aacaaatggc aagttcaagt cagtagacga actcaagaca gtctctggca ttggtgctaa
    41041 gaccatagaa aagctaaaag actatgttac agtggattaa gaacatcccc attcccctaa
    41101 tttacctgag ttttttgtta ctttggtttt attacgctat tttctcagca tcctatcttg
    41161 ctttgttggg ctttgttttt ctgctagtct gtctctttat ccaatttccg tggaaatctg
    41221 ctggtaaagt tctaataatt tgcggaatct ttggattttg gtttgttttt caaaattggc
    41281 aacagagtca agcgagtcaa aatctggcgg attctgttga aagggtacgg attttacctg
    41341 atactattaa ggttaatggt gatagtctat cctttcgtgg caagtctaac ggtcgtgctt
    41401 tccaagtcta ttataaactc cagtccgagg aggagaaaga agcctttcaa gctttaaccg
    41461 acctgcatga gataggacta gaagggaagc tttcggagcc agaagggcag agaaattttg
    41521 gtggctttaa ttaccaagcc tatctgaaga ctcagggaat ttaccagact ctcaatatca
    41581 aaacaatcca gtcacttcaa aagattggca gttgggatat aggagaaaac ttgtccagtt
    41641 tacgtcgaaa ggctgtggtt tggattaaga cgcactttcc agaccctatg cgcaattaca
    41701 tgacaggact cttgctggga catctggaca ccgactttga ggagatgaat gagctttatt
    41761 ccagtctagg aattatccac ctctttgccc tatctggtat gcaggtaggt tttttcatga
    41821 atggatttaa gaaacttctc ttgcgattgg gcttgaccca agaaaagttg aaatggctga
    41881 cttatccctt ttcccttatc tatgcgggac taactggatt ttcagcatcg gttattcgca
    41941 gtctcttgca aaagctactg gctcaacatg gggttaaggg cttggataat tttgccttga
    42001 cggtgcttgt cctctttatt gtcatgccaa actttttctt gacagcagga ggagtcttgt
    42061 cctgcgctta tgcttttatc ctgaccatga ccagcaaaga aggggagggg ctcaaggctg
    42121 ttactagtga aagtctagtc atctccttgg gcatattgcc cattctatcc ttctattttg
    42181 cggaatttca accttggtct atccttttga cctttgtctt ttcctttcta tttgacttga
    42241 ccttcttacc gctcttgtct attttatttg tcctttcctt tctctatcca gtcattcagc
    42301 tgaactttat ctttgaatgg ttggagggca ttattcgctt ggtgtcacag gtgacaagta
    42361 gacctctggt ctttggacaa cccaatgcat ggtttttaat cctattgtta atttccttgg
    42421 ctttggtcta tgatttgaga aaaaacatta aaaagctaac ggtattgtgc ttattgatta
    42481 cagggctctt tctcctgacc aagcatccac tggaaaatga aatcaccatg ctggatgtgg
    42541 ggcaaggaga aagtattttc ctacgggatg taactgggaa aaccattctc atagatgtag
    42601 gtggtaaggc agaatcttat aagaaaatcg aaaaatggca agaaaagatg acgaccagca
    42661 atgcccagcg aaccttgatt ccctatctca aaagtcgagg agtagctaag attgaccagc
    42721 taattttgac taacacggac aaggagaatg ttggagattt gtcagaggtg accaaggctt
    42781 tccatgtagg ggagattcta gtatcaaaag acagtctgaa acagaaggaa tttgtggcag
    42841 aactacaggc gactcaaaca aaggtgcgta gtatgacagt aggggagaac ttgcccattt
    42901 ttggaagtca gttagaagtt ctatctccaa ggaaaatggg agatggagga cacgatgata
    42961 ccctagttct gtatgggaaa ttcttggata agcaatttct cttcacggga aatttggagg
    43021 agaaaggaga gaaggacttg ctgaagcact atccagactt gaaagtaaat gttttgaaag
    43081 ctagccaaca tggcaataaa aaatcatcaa gtccagcctt tctagaaaaa ctcaaaccag
    43141 agcttactct tatctcagtt ggaaagagca atcgaatgaa actcccccat caggaaacat
    43201 tgacacgact ggaaggtatc aatagcaaag tttatcgaac tgaccagcaa ggagctatac
    43261 gttttaaggg gttggatagt tggaaaatcg aaagtgttcg ataggaagga taaatgttgt
    43321 agattagtga aataaactaa aaatttgttg cataataatg ataaaaatgg tataatgaaa
    43381 acgtattcaa tattgaggat ataaaatcat taaaaatcat taaaaattag caaaaatcgt
    43441 ttaattagtt agtttataat ctattggtct tcttcagtcc agtgtatctg ctgtgacagt
    43501 cactaaaagt tacaagtatg attggaatac ggtttgggaa tatagtacca actatcacga
    43561 ccatcagtat gcttggattc cgtcatggtc tcgttatgac agctattctg agtataaagt
    43621 tggcggaggc tggaactacg gtcgttatga ggtcataaac tattacagcg gaggctatta
    43681 attcttaaag agtgagaaaa aggagggcta gatatgttgc agcttactca tgtgacctta
    43741 aaaacgcgac aagtcatctt gcaagatgtg gatttcacct ttaaaaaggg tagggtttat
    43801 ggtcttcttg ctatcaatgg ctctggaaag acgaccctat tccgtgccat tagcaattta
    43861 attcccataa gtagtggaaa tatcgcagcc cctccttctt tattttatta tgagagtatt
    43921 gaatggctgg atggaaactt aagtgggatg gactaccttc gtcttatcaa aaacatctgg
    43981 aagtcaggtc tgaacttgag ggatgaaatc gcctattggg aaatgtctga ctatatcagt
    44041 cttcccattc gcaagtattc cttaggcatg aagcaacgct tggtgattgc catgtatttc
    44101 ctcagtcagg ccaaatgctg gctcatggat gagattacaa atggcttaga tgagtattat
    44161 cgacagaagt tttttgatag gctagcacaa atcgatagac aagaacagct ggttctttta
    44221 agttcccact ataaggaaga gttggttgat gtctgcgata gagtagtaac cattcatcag
    44281 gggcagatag aagaggttta gtttatgaaa gatgttagtc tatttttatt gaaaaaagtt
    44341 ttcaaaagcc gcttaaactg gattatctta gctttatttg tatctgtact cggtgttact
    44401 ttttatttta atagtcagac tgcaaactca gtcagcttgg agagagagtt ggaaactcgt
    44461 cttgtagacc gtgagagagt catcaatgaa tatgaagcga aactttccca aatgtctgac
    44521 accagctcgg aggaatacca gtttgctaaa agtaatttag aattgcaaaa aaatcttttg
    44581 aagcgaaaga cagaaattct gactttatta aaagaagggc gctggaaaga ggcctactat
    44641 ctgcagtggc aagatgaaga gaagaattat gaatttgtat caaatgaccc gactgctagc
    44701 tctggcttaa aaatgggggt tgaccgcgaa cggaagattt accaagccct gtatcccttg
    44761 aacataaaag cacatacttt ggagtttccg acccacggga ttgatcagat tgtctggatt
    44821 ttagaggtta tcatcccaag cttgtttgtg gttgcgatta tttttatgct aacacaacta
    44881 tttgcagaaa gatatcaaaa tcatctggac acagctcact tatatcctgt ttcaaaagtg
    44941 acatttgcaa tatcctctct tggagttgga gtgggatatg taactgtgct gtttatcgga
    45001 atctgtggct tttcttttct agtgggaagt ctgataagtg gttttggaca gttagattat
    45061 ccctacccaa tttatagctt agtgaatcaa gaagtaacta ttgggaaaat acaagatgta
    45121 ttatttcctg gcttgctctt agctttctta gcctttatcg tcattgtgga agttgtgtac
    45181 ttgattgctt actttttcaa gcaaaaaatg cctgtcctct ttctttcact cattgggatt
    45241 gttggcttat tgtttggcat ccaaaccatt cagcctcttc aaaggattgc acatctgatt
    45301 ccctttactt acttgcgttc agtggagatt ttatctggaa gattatctaa gcagattgat
    45361 aatgtcgatc taaattggag catggggatg gtcttacttc cttgcctgat tatccttttg
    45421 ttagtgggga ttctatttat cgaaagatgg ggaagttcac gtaaaaagga agtttttaaa
    45481 gtttagtttt cctataggga agctaaagaa aaactaacat agggagagaa tcaacttggt
    45541 tctctctttt tgattcgaaa accaaaccaa aatacaaaca caaacttttc aaaaaataac
    45601 tttttatctt gacaagagct agaaaacttg gtatcatata aaagttgaga aaagcagaag
    45661 tgagagcttc tcgccttgtg acattaagtt gcctggccct acggatgaaa agtttcgaag
    45721 aaacgctatc ataacgtgcg ggcttgtata tttacgagtc cgctattgtt tttctctaat
    45781 aaaacaaaag aggtgaaaac catagcaaag caagacttat tcatcaatga tgagattcgt
    45841 gtacgtgaag ttcgcttgat tggtcttgag ggagaacagc taggcatcaa gccactcagt
    45901 gaagcgcaag ctttggctga taacgctaat gttgacctag tattgattca accccaagcc
    45961 aaaccgcctg ttgcaaaaat tatggactac ggtaagttca aatttgagta ccagaagaag
    46021 caaaaagaac aacgtaaaaa acaaagcgtt gttactgtga aagaagttcg tctaagtcca
    46081 acaattgaca agggtgactt tgatacaaaa cttcgtaatg cacgtaagtt ccttgaaaaa
    46141 ggaaataaag ttaaggtatc tattcgcttt aagggtcgta tgattaccca taaagagatt
    46201 ggtgcaaaag ttttagccga gtttgctgaa gcaactcaag atattgccat catcgaacaa
    46261 cgtgctaaaa tggatggacg tcaaatgttc atgcaattgg cgccagcgac tgacaaaaaa
    46321 taattgtcag aaagttaaat aaaggagaaa aaatcatgcc aaaacaaaaa acacaccgcg
    46381 catcagctaa acgtttcaaa cgtacaggtt ctggtggact taaacgtttc cgtgcttaca
    46441 cttctcaccg tttccacgga aaaactaaga aacaacgtcg tcatcttcgt aaagcatcta
    46501 tggtgcattc aggagattac aaacgtatca aagcaatgct tactcgcttg aaataatagc
    46561 ttgactgtaa gtaaacaatt ctaggaaata tttggaggaa ataaaacatg gcacgtgtta
    46621 aaggtggcgt tgtatcacgc aaacgtcgta aacgtattct taaattagca aaaggttact
    46681 atggagctaa acacatcttg ttccgtactg caaaagaaca agtaatgaac tcttactact
    46741 atgcataccg tgaccgtcgt cagaaaaaac gtgacttccg caaattgtgg atcactcgta
    46801 tcaatgcggc agctcgtatg aacggacttt catactcaca attgatgcat ggtttgaaat
    46861 tggctgagat cgaagttaac cgtaaaatgc ttgctgactt ggctgttaac gatgcagtag
    46921 ctttcacagc tcttgcagat gcagctaaag caaaacttgg taaataacaa ctgatgagac
    46981 tggaacagga ttgtccagtc tttttaaaaa taaaggagaa aagtatggct tcaaaaatgc
    47041 tacacacttg cttacgagta gaaaatcttg aaaaatcaat tgcattctat caagatgctt
    47101 ttggttttaa agaattgcgt cgcagagatt ttccagacca tgccttcacg attgtctatc
    47161 taggtcttga gggtgatgat tatgagttgg agttgactta taactacgat cacggtccgt
    47221 atgtggttgg tgatgggttt gcccatatcg ccctcagtac acctgatctt gaggcacttc
    47281 atcaagaaca cagtgcaaaa ggctatgaag tgactgagcc aaatggtcta ccaggaacta
    47341 caccaaacta ttactttgtc aaagaccctg atggatataa ggtcgaagtc attcgtgaaa
    47401 aataatttcg tgcaagttaa gtagaatatt cgttttctac ttgacttttt ttagctgaaa
    47461 gagtatacta ataaaaattc aacctttaag gggagtccag agagactcac aaggtgtcag
    47521 ataaaagaat agtgcaattt tctagaggag actttttgag tgtgctctct tgtgttgtac
    47581 gattttaact gaggccttgc actagcaagg tcttttcttt atctggtccc cttaaaattt
    47641 aaggaggaaa agttatgaat cccacatgta agaagcgttt gggtgtcatt cggttggaaa
    47701 ccatgaaggt ggttgcacaa gaggaaatcg cgccagcaat ctttgaatta gttctagaag
    47761 gagaaatggt tgaagccatg cgagcaggcc aatttcttca tctgcgtgta ccggacgatg
    47821 cccatctctt acgtcgtcct atttcaattt cgtctattga caaggcaaac aagcagtgtc
    47881 acctcattta tcggattgag ggagctggga ctgcaatttt ttcaacctta agtcagggag
    47941 acactcttga tgtgatgggg cctcagggaa atggttttga cttgtctgac cttgatgagc
    48001 agaatcaggt tctccttgtt ggtggtggga ttggtgttcc acccttgctt gaggtggcca
    48061 aggaattgca tggacgtgga gtgaaagtag tgacagtcct cggttttgct aataaggatg
    48121 ctgttatttt gaaaacggaa ttggctcagt atggtcaggt ctttgtaacg acagatgatg
    48181 gttcttatgg catcaaggga aatgtttccg ttgttatcaa tgatttagat agtcagtttg
    48241 atgctgttta ctcgtgtggg gctccaggaa tgatgaagta tatcaatcaa acctttgatg
    48301 atcacccaag agcctattta tctctggaat ctcgtatggc ttgtgggatg ggagcttgct
    48361 atgcctgtgt tctaaaagta ccagaaagcg agacggtcag ccaacgcgtc tgtgaagatg
    48421 gtcctgtttt ccgcacagga acagttgtat tataaggaga aaattatgac tacaaatcga
    48481 ttacaagtgt ctctacctgg tttggatttg aaaaatccga ttattccagc atcaggctgt
    48541 tttggctttg gacaagagta tgccaagtac tatgatttag accttttagg ttctattatg
    48601 atcaaggcga caacccttga accacgtttt gggaatccaa ctccaagagt ggcagagacg
    48661 cctgctggta tgctcaatgc aattggcttg caaaatcctg gtttagaggt tgttttggct
    48721 gaaaagctac cttggctgga aagagaatat ccaaatcttc ctattattgc caatgtagct
    48781 ggtttttcaa aacaagagta tgcagctgtt tctcatggga tttccaaggc aactaatgta
    48841 aaagctatcg agctcaatat ttcttgtccc aatgttgacc actgtaatca tggacttttg
    48901 attggtcaag atccagattt ggcttatgat gtggtgaaag cagctgtgga agcctcagaa
    48961 gtgccagttt atgtcaaatt aaccccgagt gtgaccgata tcgttactgt cgcaaaagct
    49021 gcagaagatg cgggagcaag tggcttgacc atgatcaata ctctggttgg aatgcgcttt
    49081 gacctcaaaa ccagaaaacc aatcttggcc aatggaacag gtggaatgtc aggtccagca
    49141 gtctttccag tagccctcaa actcatccgc caagtagccc aaacaacaga cctgcctatc
    49201 attggaatgg ggggagtgga ttcggctgaa gctgccctag aaatgtatct ggctggggca
    49261 tctgctatcg gagttggaac agctaacttt accaatcctt atgcctgtcc tgtcatcatc
    49321 gaaaatttac caaaagtcat ggataaatac ggtattagca gtctggaaga actccgtcag
    49381 gaagtaaaag agtctctgag gtaaactgca atcaatctgt tcttgatttt ttattagttt
    49441 gtaatatgaa tttaggagaa ttttggtaca ataaaataaa taagaacaga ggaagaaggt
    49501 taatgaagaa agtaagattt atttttttag ctctgctatt tttcttagct agtccagagg
    49561 gtgcaatggc tagtgatggt acttggcaag gaaaacagta tctgaaagaa gatggcagtc
    49621 aagcagcgaa tgagtgggtt tttgatactc attatcaatc ttggttctat ataaaagcag
    49681 atgctaacta tgctgaaaat gaatggctaa agcaaggtga cgactatttt tacctcaaat
    49741 ctggtggcta tatggccaaa tcagaatggg tagaagacaa gggagccttt tattatcttg
    49801 accaagatgg aaagatgaaa agaaatgctt gggtaggaac ttcctatgtt ggtgcaacag
    49861 gtgccaaagt aatagaagac tgggtctatg attctcaata cgatgcttgg ttttatatca
    49921 aagcagatgg acagcacgca gagaaagaat ggctccaaat taaagagaag gactattatt
    49981 tcaaatccgg tggttatcta ctgacaagtc agtggattaa tcaagcttat gtgaatgcta
    50041 gtggtgccaa agtacagcaa ggttggcttt ttgacaaaca ataccaatct tggttttaca
    50101 tcaaagaaaa tggaaactat gctgataaag aatggatttt cgagaatggt cactattatt
    50161 atctaaaatc cggtggctac atggcagcca atgaatggat ttgggataag gaatcttggt
    50221 tttacctcaa gtttgatggg aaaatagctg aaaaagaatg ggtctacgat tcttatagtc
    50281 aagcttggta ctacttcaaa tccggtggtt acatgacagc caatgaatgg atttgggata
    50341 aggaatcttg gttttatctc aaatctgatg ggaaaatagc tgaaaaagaa tgggtctacg
    50401 attctcatag tcaagcttgg tactacttca aatctggtgg ttacatgaca gccaatgaat
    50461 ggatttggga taaggaatct tggttttacc tcaagtctga tggcaaaatg gctgaaaaag
    50521 aatgggtcta cgattctcat agtcaagcct ggtactactt caaatctggt ggctacatgg
    50581 cgaaaaatga gacagtagat ggttatcagc ttggaagcga tggtaaatgg cttggaggaa
    50641 aagctacaaa taaaaatgct gcttactatc aagtagtgcc tgtcacagcc aatgtttatg
    50701 attcagatgg tgaaaagctt tcctatatat cgcaaggtag tgtcgtatgg ctagataagg
    50761 atagaaaaag tgatgacaag cgcttggcta ttactatttc tggtttgtca ggctatatga
    50821 aaacagaaga tttacaagcg ctagatgcta gtaaggactt tatcccttat tatgagagtg
    50881 atggccaccg tttttatcac tatgtggctc agaatgctag tatcccagta gcttctcatc
    50941 tttctgatat ggaagtaggc aagaaatatt attcggcaga tggcctgcat tttgatggtt
    51001 ttaagcttga gaatcccttc cttttcaaag atttaacaga ggctacaaac tacagtgctg
    51061 aagaattgga taaggtattt agtttgctaa acattaacaa tagccttttg gagaacaagg
    51121 gcgctacttt taaggaagcc gaagaacatt accatatcaa tgccctttat ctccttgccc
    51181 atagtgccct agaaagtaac tggggaagaa gtaaaattgc caaagataag aataatttct
    51241 ttggcattac agcctatgat acgacccctt acctttctgc caagacattt gatgatgtgg
    51301 ataagggaat tttaggtgca accaagtgga ttaaggaaaa ttatatcgat aggggaagaa
    51361 ctttccttgg aaacaaggct tctggtatga atgtggaata tgcttcagac ccttattggg
    51421 gcgaaaaaat tgctagtgtg atgatgaaaa tcaatgagaa actaggtggc aaagattagt
    51481 actataagtg aatatgattt gagtgaatag taagttaaaa atcctgattt caagtaaaat
    51541 caggattttt tcatggatgc aatttttttg gagtctggtg tgacgcggag ggtcttttgt
    51601 cctgtgtaag tgacaaagcc gggttttcca ccagttggtt tattgagttt tttgacttca
    51661 atcatatcta cctgcaccag attcgacagg cgcccttgag agaagtaggc agctaactct
    51721 gctgcgtctg tcttgactgc atcagatggg tcaagatttc ctgagatgac aacatggctt
    51781 ccaggaatgt ccttagcatg gaaccaaagt tcctccttgc gggccatttt aaaggtcaat
    51841 tcctcatttt gaagattgtt tcgtccgaca tagatgatgg ttttgccatc gcttgctaga
    51901 tattgttcta gttttttgcg tttctggatt ttctcccgtt gtcttctgcg gataaaacct
    51961 gtttgaatca attcttcacg gatttcagcg atttcttcca gtccagcttg gttgaggacg
    52021 gtttctacac tttccagata gagaatagtg gctttggttt cttcaatcaa atcagtcaag
    52081 tatttgacag cttctttgag tttctgatac cgtttaaaat agcgttgggc attctggttg
    52141 ggagtcagag ccttatcaag cgcaatcatg ataggttggt tggtatagta gttgtctagg
    52201 ataacctggt cttggtcgtt aggcacttgg tggaggaagg ttgtcagcaa ttctcctttt
    52261 tgacgaaatt cttcagcgtt gtctgtcgcc agtaactctt tttcctgttt tttgagtttg
    52321 tgtcggtttt tctgaagttc attttcaaca cgacgaatca gttcactggc ctgctgtttg
    52381 acgcggtcgc gctcagcctt atccttatag taggtgtcca acaaatcaga aagatttgca
    52441 aaaggctctc ccacctgatt tgcaaaagga actggactga aggaagtctc agtcaagcat
    52501 ggcttgtttt cttgattgaa aaaatttcgg aaagcggaaa gtttttcact aaccagtatc
    52561 ctttccaatt catttgccgt atcgcgtccc agaccttgaa agaggctttg aagatttttt
    52621 gctgttagtt cttgggtttg caggatttca aagagctttt catccttgat agtaaaagga
    52681 ttgagagatt ctgtacttgg cggagcgata taggtcgatc ctggaagtaa ggtgcggtag
    52741 ctattttgtg aaaaaccgac gtgtttgata acttcgagga ttttatgact gcttttatcg
    52801 accagtagaa tattactgtg tttccccata atttcaataa tcaaggtagc ctggatatgg
    52861 tctccaatct cgtttttatt ggaaactgta atttccacaa tacggtcatt ttccacttgc
    52921 tcaatcgact caatcagggc cccctgcaaa tactttctca aaaccatgat aaaggtagaa
    52981 ggttgagctg gattttcaaa agtcgtttgg gtcagctgaa tgcgtccaaa aactggatgg
    53041 gcagaaagga gcaggcgatg gctttggcga ttgctgcgga tttgcaagac caactcttgt
    53101 tcaaaaggct gattgatttt ctggatgcga ccattcacta attcgcttcg caattcctca
    53161 actatgtggt gtaaaaaaaa tccgtcaaat gacatcgttc tctccttgtg attgtattcc
    53221 atagtattat atcaaaaagg tagaataaaa tcatggaaat gtggtataat aaagccaagt
    53281 aaagagaaaa gagaagcaca tgtatattga aatggtagat gaaactggtc aagtttcaaa
    53341 agaaatgttg caacaaaccc aagaaatttt ggaatttgca gcccaaaaat taggaaaaga
    53401 agacaaggag atggcagtca cttttgtgac caatgagcgt agtcatgaac ttaatctgga
    53461 gtaccgtaac accgaccgtc cgacagatgt catcagcctt gagtataaac cagaattgga
    53521 aattgccttt gacgaagagg atttgcttga aaattcagaa ttggcagaga tgatgtctga
    53581 gtttgatgcc tatattgggg aattgttcat ctctagcgat aaggctcatg agcaggccga
    53641 agaatatggt cacagctttg agcgtgagat gggcttcttg gcagtacacg gctttttaca
    53701 tattaacggc tatgatcact acactccgga agaagaagcg gagatgttcg gtttacaaga
    53761 agaaattttg acagcctatg gactcacaag acaataaacg aaaatggaaa aatcgtgact
    53821 tgatatccag tttagaattt gctttgacag gtatttttac tgctatcaag gaagaacgca
    53881 atatgcgaaa acacgcagtg acggctctag tggtcatcct tgcaggtttt ggttttcagg
    53941 tgtcacgaat cgaatggctc tttctcctat tgagcatttt cttggtagta gcctttgaga
    54001 ttctcaactc tgctattgaa aatgtggtgg atttggccag tcactatcac ttttccatgc
    54061 tggctaaaaa tgccaaggat atggcggctg gcgcggtatt agtggtttct ctttttgcag
    54121 ccttaacagg cgcattgatt tttctcccac gaatctggga tttattattt taaacagtaa
    54181 gaggaaatta tgacttttaa atcaggcttt gtagccattt taggacgtcc caatgttggg
    54241 aagtcaactt ttttaaatca cgttatgggg caaaagattg ccatcatgag tgacaaggcg
    54301 cagacaacgc gcaataaaat catgggaatt tacacgactg ataaggagca aattgtcttt
    54361 atcgacacac cagggattca caaacctaaa acagctctcg gagatttcat ggttgagtct
    54421 gcctacagta cccttcgcga agtggacact gttcttttca tggtgcctgc tgatgaagcg
    54481 cgtggtaagg gggacgatat gattatcgag cgtctcaagg ctgccaaggt tcctgtgatt
    54541 ttggtggtga ataaaatcga taaggttcat ccagaccagc tcttgtctca gattgatgac
    54601 ttccgtaatc aaatggactt taaggaaatt gttccaatct cagcccttca gggaaataac
    54661 gtgtctcgtc tagtggatat tttgagtgaa aatctggatg aaggtttcca atatttcccg
    54721 tctgatcaaa tcacagacca tccagaacgt ttcttggttt cagaaatgct tcgcgagaaa
    54781 gtcttgcacc taactcgtga agagattccg cattctgtag cagtagttgt tgactctatg
    54841 aaacgagacg aagagacaga caaggttcac atccgtgcaa ctatcatggt cgagcgcgat
    54901 agccaaaaag ggattatcat cggtaaaggt ggcgctatgc ttaagaaaat cggtagcatg
    54961 gcccgtcgtg atatcgaact catgctagga gacaaggtct tcctagaaac ctgggtcaag
    55021 gtcaagaaaa actggcgcga taaaaagcta gatttggctg actttggcta taatgaaaga
    55081 gaatactaag tagaggtagg ctcatgcctg cttcttgttt ttacagaagg aggacttatg
    55141 cctgaattac ctgaggttga aaccgtttgt cgtggcttag aaaaattgat tataggaaag
    55201 aagatttcga gtatagaaat tcgctacccc aagatgatta agacggattt ggaagagttt
    55261 caaagggaat tgcctagtca gattatcgag tcaatgggac gtcgtggaaa atatttgctt
    55321 ttttatctga cagacaaggt cttgatttcc catttgcgga tggagggcaa gtatttttac
    55381 tatccagacc aaggacctga acgcaagcat gcccatgttt tctttcattt tgaagatggt
    55441 ggcacgcttg tttatgagga tgttcgcaag tttggaacca tggaactctt ggtgcctgac
    55501 cttttagacg cctactttat ttctaaaaaa ttaggtcctg aaccaagcga acaagacttt
    55561 gatttacagg tctttcaatc tgcccttgcc aagtccaaaa agcctatcaa atcccatctc
    55621 ctagaccaga ccttggtagc tggacttggc aatatctatg tggatgaggt tctctggcga
    55681 gctcagattc atccagctag accttcccag actttgacag cagaagaagc gactgccatt
    55741 catgaccaga ccattgctgt tttgggccag gctgttgaaa aaggtggctc caccattcgg
    55801 acttatacca atgcctttgg ggaagatgga agcatgcagg actttcatca ggtctatgat
    55861 aagactggtc aagaatgtgt acgctgtggt accatcattg agaaaattca actaggcgga
    55921 cgtggaaccc acttttgtcc aaactgtcaa aggagggact gatgggaaaa atcatcggaa
    55981 tcactggggg aattgcctca ggtaagtcaa ctgtgacaaa ttttctaaga cagcaaggct
    56041 ttcaagcagt ggatgccgac gcagtcgtcc accaactaca gaaacctggt ggtcgtctgt
    56101 ttgaggctct agtacagcac tttgggcaag aaatcattct tgaaaacgga gaactcaatc
    56161 gccctctcct agctagtctc atcttttcaa atcctgaaga gcaaaaatgg tctaatcaaa
    56221 ttcaagggga gattatccgt gaggaactgg ctactttgag agaacagttg gctcagacag
    56281 aagagatttt cttcatggat attcccctac tttttgagca ggactacagc gattggtttg
    56341 ctgagacttg gttggtctat gtggaccgag atgcccaagt agaacgctta atgaaaaggg
    56401 accagttgtc caaagatgaa gctgagtctc gtctggcagc ccagtggcct ttagaaaaaa
    56461 agaaagattt ggccagccag gttcttgata ataatggcaa tcagaaccag cttcttaatc
    56521 aagtgcatat ccttcttgag ggaggtaggc aagatgacag agattaactg gaaggataat
    56581 ctgcgtattg cctggtttgg taattttctg acaggagcca gtatttcttt ggttgtacct
    56641 tttatgccca tcttcgtgga aaatctaggt gtagggagtc agcaagtcgc tttttatgca
    56701 ggcttagcaa tttctgtctc tgctatttcc gcggcgctct tttctcctat ttggggtatt
    56761 cttgctgaca aatacggccg aaaacccatg atgattcggg caggtcttgc tatgactatc
    56821 actatgggag gcttggcctt tgtcccaaat atctattggt taatctttct tcgtttacta
    56881 aacggtgtat ttgcaggttt tgttcctaat gcaacggcac tgatagccag tcaggttcca
    56941 aaggagaaat caggctctgc cttaggtact ttgtctacag gcgtagttgc aggtactcta
    57001 actggtccct ttattggtgg ctttatcgca gaattatttg gcattcgtac agttttctta
    57061 ctggttggta gttttctatt tttagctgct attttgacta tttgctttat caaggaagat
    57121 tttcaaccag tagccaagga aaaggctatt ccaacaaagg aattatttac ctcggttaaa
    57181 tatccctatc ttttgctcaa tctcttttta accagttttg tcatccaatt ttcagctcaa
    57241 tcgatcggcc ctattttggc tctttatgta cgcgacttag ggcagacaga gaatcttctt
    57301 tttgtctctg gtttgattgt gtccagtatg ggcttttcca gtatgatgag tgcaggagtc
    57361 atgggcaagc taggtgacaa ggtgggcaat catcgtctct tggttgtcgc ccagttttat
    57421 tcagtcatca tctatctcct ctgtgccaat gcctctagcc cccttcaact aggactctat
    57481 cgtttcctct ttggcttggg aaccggtgcc ttgattcccg gggttaatgc cctactcagc
    57541 aaaatgactc ccaaagccgg catttcgagg gtctttgcct tcaatcaggt attcttttat
    57601 ctgggaggtg ttgttggtcc catggcaggt tctgcagtag caggtcaatt tggctaccat
    57661 gctgtctttt atgcgacaag cctttgtgtt gcctttagtt gtctctttaa cctgattcaa
    57721 tttcgaacat tattaaaagt aaaggaaatc tagtgcgagt aaaaattaat ctcaaatgct
    57781 cctcttgtga cagtatcaat tacctaacca gtaaaaattc aaaaacccat ccagacaaga
    57841 ttgaggtttt aaagtattgt cccaaggaaa gaaaagtaac cctacatctt gaatctaagt
    57901 agattttatg gtaaaataat agggatttta aggagtttga tatgtataac ctattattaa
    57961 ccattttatt agtattatct gttgtgattg tgattgcaat tttcatgcaa ccaaccaaaa
    58021 accaatccag caatgtattt gatgccagtt caggtgattt gtttgaacgc agtaaagctc
    58081 gcggttttga agctgtaatg cagcgtttga cagggatttt agtctttttc tggctagcca
    58141 ttgccttagc attgacggta ttatcaagta gataagaaaa taatgggcag gactaggtct
    58201 ttgcctcttt ttatttttaa aggatgtttg agaaggtttt acagtaaaag aaaattaaaa
    58261 aatctagaaa gaaaatatga aagatagaat aaaagaatat ttacaagaca agggaaaggt
    58321 gactgttaat gatttggctc aggctttggg aaaagacagt tccaaggatt ttcgtgagtt
    58381 gattaaaacc ttgtccttaa tggaaagaaa gcaccagatt cgctttgaag aagatggcag
    58441 tctgaccttg gaagccaaga aaaaacatga gattaccctc aaggggattt ttcatgccca
    58501 taaaaatggt tttggttttg tcagcctaga aggggaagag gacgaccttt ttgtagggaa
    58561 aaatgatgtc aactatgcta ttgatggtga taccgtcgag gtagtgatta agaaagtcgc
    58621 tgaccgcaat aagggaacag cagcagaagc caaaattatt gatatcctag aacacagttt
    58681 gacaacagtt gtcgggcaaa tcgttctgga tcaggaaaag cctaagtatg ctggctatat
    58741 tcgttcaaaa aatcagaaaa tcagtcaacc gatttatgtt aagaaaccag ccctaaagtt
    58801 agaaggaaca gaagttctca aggtctttat cgataaatac ccaagcaaga aacatgattt
    58861 ctttgtcgcg agtgttctcg atgtagtggg acactcaacg gatgtcggaa ttgatgttct
    58921 tgaggtcttg gaatcaatgg acattgtatc cgagtttcca gaagctgttg ttaaggaagc
    58981 agaaagtgtg cctgatgctc cgtctcaaaa ggatatggaa ggtcgtctgg atctaagaga
    59041 tgaaattacc tttaccattg acggtgcgga tgccaaggac ttggacgatg cagtgcatat
    59101 caaggctctg aaaaatggca atctggagct tggagttcac atcgcagatg tttcttatta
    59161 tgtgaccgag gggtctgccc ttgacaagga agcccttaac cgtgcgactt ctgtttacgt
    59221 gacagaccga gtggtgccaa tgcttccaga acgactatca aatggcatct gctctctcaa
    59281 tccccaagtt gaccgcctga cccagtctgc tattatggag attgataaac atggtcgtgt
    59341 ggtcaactat accattatac aaacagttat caagaccagt tttcgtatga cctatagcga
    59401 tgtcaatgat atcctagctg gcgatgaaga aaagagaaaa gaatatcata aaattgtacc
    59461 aagtatcgaa ctcatggcca agcttcatga aactttagaa aacatgcgtg tgaaacgtgg
    59521 agccctcaat tttgatacca atgaagcgaa gattttagtg gataaacaag gtaagcctgt
    59581 tgatatcgtt cttcggcagc gtggtattgc cgagcggatg attgagtctt ttatgttgat
    59641 ggctaatgaa acagttgccg aacatttcag caagttggat ttgcctttta tctatcgaat
    59701 tcacgaggag cctaaggctg aaaaggttca gaagtttatt gattatgctt cgagttttgg
    59761 cttgcgcatt tatggaactg ccagtgagat tagtcaggag gcacttcaag acatcatgcg
    59821 tgctgttgag ggagaacctt atgcagatgt attgtccatg atgcttcttc gctctatgca
    59881 gcaggctcgt tattcggagc acaatcacgg ccactatgga ctagctgctg actattatac
    59941 tcactttacc agtccaattc gtcgttatcc agaccttctt gttcaccgta tgattcggga
    60001 ttacggccgt tctaaggaaa tagcagagca ttttgaacaa gtgattccag agattgcgac
    60061 ccagtcttcc aaccgtgaac gtcgtgccat agaagctgag cgtgaagtcg aagccatgaa
    60121 aaaggctgag tatatggaag aatacgtggg tgaagagtat gatgcagttg tatcaagtat
    60181 tgtcaaattc ggtctctttg tcgaattgcc aaacacagtt gaaggcttga ttcacatcac
    60241 taatctacct gaattttatc atttcaatga gcgtgatttg actcttcgtg gagaaaaatc
    60301 aggtatcact ttccgagtgg gtcagcagat ccgtatccgt gttgaaagag cggataaaat
    60361 gactggagag attgattttt cattcgtacc tagtgagttt gatgtgattg aaaaaggctt
    60421 gaaacagtct agtcatagtg gcagagggcg tggttcaaat cgtcgttcgg ataagaagga
    60481 agacaagaga aaatcaggac gctcaaatga taagcgtaag cattcacaaa aagacaagaa
    60541 gaaaaaagga aagaaacctt tttacaagga agtagctaag aaaggagcca agcatggcaa
    60601 agggcgaggg aaaggtcgtc gcacaaaata aaaaggcacg ccacgactat acaatcgtag
    60661 atacgctaga ggcagggatg gtcctgactg gaactgaaat caagagtgta cgagctgctc
    60721 gaattaatct caaggatggc tttgctcaag tgaaaaatgg agaagtttgg ctgagtaatg
    60781 ttcatatcgc gccttacgaa gagggcaata tctggaacca ggaaccagaa cgtcgtcgta
    60841 aactcctgct ccataaaaag caaattcaaa aattggaaca agagaccaaa gggacaggaa
    60901 tgaccttagt tccccttaag gtctatataa aagatggcta cgctaagctt cttttaggac
    60961 ttgccaaagg gaagcatgac tatgacaaac gggagtctat caaacgtcgt gagcaaaatc
    61021 gagatatcgc gcgtgtgatg aaagctgtta atcagcgata aaaagaggaa ttgaaaatgg
    61081 aaaaattagt tgcctataaa cgcatgcctt tgtggaataa acaaacaatg cctgaagctg
    61141 ttcagcaaaa gcacaataca aaagttggga cttgggggaa aattactgtc ttgaagggag
    61201 ctctcaagtt tattgaattg acagaagaag gggaagttct agctgaacac ctctttgaag
    61261 caggggcaga caatccaatg gcccaacctc aagcctggca ccgagtggaa gctgccacag
    61321 atgatgtgga atggtacttg gaattttatt gtaaacctga ggattatttt gctaaaaaat
    61381 acaataccaa tcctgttcat tcagaggtcc tagaggccat gcagacagtg aaacaaggga
    61441 aagctttgga tttaggttgt ggtcaggggc gtaattctct ttttctagcc cagcaagatt
    61501 ttgatgtgac ggctgtagat caaaatggac tagctcttga aatcttgcaa agcattgtgg
    61561 agcaggaaga tttggacatg cctgttggcc tttacgatat caattcagct agcattgaac
    61621 aagagtatga ttttatcgtt tcaacagttg ttctcatgtt tctacaagcg gaccgcattc
    61681 cagctattat tcaaaatatg caggagaaaa ccagtgttgg tggttacaac cttatcgttt
    61741 gtgccatgga cacggaggat tatccttgct cggttaactt cccattcacc tttaaagaag
    61801 gagaactggc agactattac aaggattggg aattggttaa gtacaatgaa aatccaggcc
    61861 atttgcatcg tcgcgatgag aatggcaatc gtattcaact acgctttgcg accttactag
    61921 ctaagaaaat caagtaaaca cacatgaaga ttaggaattt tcctgatctt ttttcttttt
    61981 tacgaatgat atagaaaagg agggaattca tgtttgttgc gagagatgct aggggagaat
    62041 tggtaaatgt gttagaggat aaacttgaga agcaagcata cacctgccca gcttgtggag
    62101 gccagctcca tttgcgtcaa ggaccaagtg tacggacgca ttttgcccat aaatccttaa
    62161 aagactgtga ttttttcttt gaaaatgaaa gtccagaaca cctggccaat aaggaatccc
    62221 tctatcactg gttgaaaaaa gagacaaagg ttcaattaga gtacccgctt tcagaactta
    62281 aacagattgc ggatgtattt gtaaatggca atctagctct agaagttcag tgtagtccct
    62341 tgcctcagaa agtccttaaa gagcgaagtg agggctatcg tagtcagggt taccaagtac
    62401 tgtggttgct gggtcaaaaa ctgtggctca aggagcgttt gactcgtcta cagcaaggtt
    62461 ttctttattt cagtcaaaac atgggctttt atgtttggga attagacaag gaaaaacaag
    62521 ttttaagact caaatacctg atttaccagg atctccgcgg taaactccat tatcaaatca
    62581 aggaattttc ctatggtcaa ggtagtttat tggaaatatt gcgtcttccc tataagagac
    62641 aaaaaatatc tcattttaca gtttctgagg acaaggacat ctgtcgctat atccggcaac
    62701 aactttatta tcaaaatctc ttttggatga aagaacaagc agaagcctat caaaagggag
    62761 aaaatatcct gacttatgga ctgaaagaat ggtatccaca aattcgacca atagtgggca
    62821 aatttttcca gattgaacaa gacttgacta gctattatca gcacttttat acctattacc
    62881 aaaaaaatcc tcaaaatgat tggcaaaagc tttatccacc agccttttat cagcaatatt
    62941 tcttgaaaaa tatggtagaa tagaaaggat ggaggaatct aatggtatta caaagaaatg
    63001 aaataaatga aaaagataca tgggatctat caacgatcta cccaactgac caggcttggg
    63061 aagaagcctt aaaagattta acagaacaat tggagacagt agcccagtat gaaggccatc
    63121 tcttggatag tgcggataac ctactagaaa tcactgaatt ttctcttgaa atggaacgcc
    63181 agatagagaa gctttacgtt tatgctcata tgaagaatga ccaggataca cgtgaagcta
    63241 agtatcaaga gtactatgcc aaggccatga cactctacag ccagttagac caagcctttt
    63301 cattctatga gcctgaattt atggagatta gcgaaaagca gtatgctgac tttttagaag
    63361 ctcaaccaaa gctgcaggtt tatcaacact attttgacaa gctcttgcaa ggcaaggatc
    63421 acgttctttc acaacgtgaa gaagaattat tggctggagc tggagaaatc tttggttcag
    63481 caagtgaaac cttcgctatc ttggacaatg cggatattgt gttcccttat gtcctagacg
    63541 atgatggtaa agaagttcag ctatctcatg ggatttacac acgtttgatg gagtctaaaa
    63601 aacgtgaggt tcgccgtggt gcctatcaag ctctttatgc gacttacgaa caattccaac
    63661 acacctatgc caaaaccttg caaaccaatg ttaaggtgca aaattaccgt gctaaagttc
    63721 gtaactacaa gagtgctcgt catgcagccc tcgcagcgaa ttttgttcca gaaagtgttt
    63781 atgacaattt ggtagcagca gttcgcaagc atttgccact cttacatcgc tatcttgagc
    63841 ttcgttcaaa aatcttgggg atttcagatc tcaagatgta cgatgtctac acaccgcttt
    63901 catctgttga atacagtttt acctaccaag aagccttgaa aaaagcagaa gatgctttgg
    63961 cagtcttggg tgaggattac ttgagccgtg tcaaacgtgc cttcagcgag cgttggattg
    64021 atgtttacga aaatcaaggc aagcgttcag gtgcctactc tggtggttct tacgatacca
    64081 atgcctttat gcttctcaac tggcaggaca atctggacaa tctctttact cttgttcatg
    64141 aaacaggtca cagtatgcat tcaagctata ctcgtgaaac tcagccttat gtttacggag
    64201 attactctat ctttttggct gagattgcct caactaccaa tgaaaatatc ttgacggaga
    64261 aattattgga agaagtggaa gacgacgcaa cacgctttgc tattctcaat aacttcctag
    64321 atggtttccg tggaacagtt ttccgccaaa ctcaatttgc tgagtttgaa cacgccattc
    64381 accaagcaga tcaaaatggg gaggtcttga caagcgattt cctaaataaa ctctacgtag
    64441 acttgaacca agagtattat ggtttgagta aggaagacaa tcctgaaatc caatacgagt
    64501 gggctcgcat tccacacttc tactataact actatgtata tcaatattca actggatttg
    64561 cggccgcctc agccttggct gaaaaaattg tccatggtag tcaagaagac cgtgaccgct
    64621 atatcgacta cctcaaggca ggtaagtcgg actatccact taatgtcatg agaaaagctg
    64681 gtgttgatat ggagaaggaa gactacctca acgatgcctt tgcagtcttt gaacgccgtt
    64741 taaatgagtt tgaagccctt gttgaaaaat taggattggc ataaaatggt tgaatcgtat
    64801 agtaagaatg ctaaccataa catgcgtcgt cctgtcgtca aagaagaaat tgtagacttg
    64861 atgcgtcagc gtcaaaagca ggtcacaggt tccttgaaag aattggaaga ctttgcccgc
    64921 aaggaaaata ttcctattat tccccatgaa acggttgctt atttccgttt tcttatggaa
    64981 accatgcagc ctaaaaatat tctggaaatt gggacggcta tcggtttttc agctctcttg
    65041 atggctgaac atgcgccaaa tgctaagatt acaactattg atcgtaatcc agaaatgatt
    65101 ggttttgcca aggaaaattt tgcccagttt gacagtcgca agcaaatcac tctcctagag
    65161 ggagatgcgg tggatgtctt atctacactg acagagtctt atgatttcgt ctttatggat
    65221 tctgccaagt ctaaatacat cgtctttctg ccagaaatcc tcaaacattt ggaagttggt
    65281 ggtgtggttg tcttggatga tatttttcaa ggtggtgatg ttgccaagga tattatggaa
    65341 gtccgtcgtg gtcagcgaac catttatcga ggccttcaaa aattatttga tgcaacctta
    65401 gacaatccag aactcaccgc aacattagtg cctttaggag atggtattct catgcttcgt
    65461 aaaaatgtag cagatgttca actgtctgaa agcgaatgat tttcagaaaa atttaagaaa
    65521 aaatagtaaa atagatagag taacacttat ctcaaaggag tagacatgaa gaaaaaatta
    65581 ttggcaggtg ccatcacact attatcagta gcaactttag cagcttgttc gaaagggtca
    65641 gaaggggcag accttatcag catgaaaggg gatgtcatca cagaacatca attttatgag
    65701 caagtgaaaa acaacccttc agcccaacaa gtcttgttaa atatgaccat ccaaaaagtt
    65761 tttgaaaaac aatatggctc agagcttgat gataaagagg ttgatgatac tattgccgaa
    65821 gaaaaaaaac aatatggcga aaactaccaa cgtgtcttgt cacaagcagg tatgactctt
    65881 gaaacacgta aagctcaaat tcgtacaagt aaattagttg agttggcagt taagaaggta
    65941 gcagaagctg aattgacaga tgaagcctat aagaaagcct ttgatgagta cactccagat
    66001 gtaacggctc aaatcatccg tcttaataat gaagataagg ccaaagaagt tctcgaaaaa
    66061 gccaaggcag aaggtgctga ttttgctcaa ttagccaaag ataattcaac tgatgaaaaa
    66121 acaaaagaaa atggtggaga aattaccttt gattctgctt caacagaagt acctgagcaa
    66181 gtcaaaaaag ccgctttcgc tttagatgtg gatggtgttt ctgatgtgat tacagcaact
    66241 ggcacacaag cctacagtag ccaatattac attgtaaaac tcactaagaa aacagaaaaa
    66301 tcatctaata ttgatgacta caaagaaaaa ttaaaaactg ttatcttgac tcaaaaacaa
    66361 aatgattcaa catttgttca aagcattatc ggaaaagaat tgcaagcagc caatatcaag
    66421 gttaaggacc aagccttcca aaatatcttt acccaatata tcggtggtgg agattcaagc
    66481 tcaagcagta gtacatcaaa cgaatagtcc aaatcaatga gtcagggaaa aaactcgact
    66541 tcaggaaaaa atgaagcaaa cattcccaca ataaaacgca tagtacaagg tttgtactga
    66601 ccccaaaagt tagacaatta atttatctga aggatttagt tctgtattgc acagggctaa
    66661 gtccttttag ttttacctta attctcttat tgttgtaata atcaatatag tctataatgg
    66721 cttgttccaa ttgattaagt gatttaaatg ttttctcata gccataaaac atttcggatt
    66781 ttaaaatgcc aaagaaagat tccatcctac cgttgtcttg gctgtttcct ttgcgtgaca
    66841 tagatgcttg aattccctta ctctctagga agcgatgata agaatcgtgt tgatattgcc
    66901 agccttggtc actatggaga atcgtattct cgtagtgctt ctctttgaat gcctgttcca
    66961 acattaacga tcaatcaatt taatcatgta cctaatatta gaattgttta tcccaaattt
    67021 atttgaaagc ttctctaagc tatatccttg ttttctaagt tcatagatct gaactttatc
    67081 atcataagtt aatttcataa taaaaacacc ccaaaagtta gattttttct gtctaacttt
    67141 tggggtgtag ttcatgtaca cctgatatga tgcgttttat aattttaaag actttttgac
    67201 cagcctcatt tttttaactt gatactcagt gaaaagcaaa gattaaacta ggaagctagc
    67261 tgtaggctgc tcaaagaaca gctttgaggt tgcagataaa acttgtgagg taaccaacat
    67321 atataatgtg aagctgacgt ggtttgaata gattttagaa gggtatgagt ctggaagttt
    67381 taatggataa tgcaagattc catagaatgg gtaagctaga gttcttatgt gaagagtttg
    67441 ggcataaact tttacctttt cctccctact catcttagta tagaaaagtg aatctaaaat
    67501 agtacataac tgcttctaaa acattcttat aaattgattt aaattctcaa atcatattat
    67561 tcagttctta tttcattttg ctctacaatc ctgttgagaa gacacgtgtt catatcaaaa
    67621 aggtattggc aagttgcaat acctttttac aaggttcttt tgtcttattt ttgtttcaac
    67681 tgactatatc tcctatggtt ctagttcaga aggctaggct ataattatga ttgataagaa
    67741 gtatcattcc aagtattgag agtgaatgtt tcaaaatcat gggtttctat aatggtcagg
    67801 ctggcatttg ctagaccgcc atctttacga agaagtggtt ctttatagcc taggagagta
    67861 cgaagactgg cagtaagatt ggcgccgtgt ccgacaatta gaatacgctc agctggacta
    67921 tcttttaatg atttgataaa ttggatggtc cgctgagttg tactatagag ggattcggct
    67981 ccgaacattc gagtgtcaaa ttgagcaaga tttgaacgaa aagcctggat ttgttgcggg
    68041 taaatagctt ccagggttgc aattttcaaa ccttctaact tcccaagttg ccattcacgg
    68101 agattaggaa cgatttctaa agaacagggg gtatggagtt gactttggat aatctcagca
    68161 gatttgaccg ctcgaggtaa atcacttgaa taaatctgat caaaaggaat ttccttgaga
    68221 tactggccaa gtcgttttag ggtttcaatg gattcaggaa gaagaggaga atcaccgcta
    68281 gcaccttgaa aacgaccttc ttggttccag agggtacgac cgtggcggac aaagtagagt
    68341 ttcattactt gatgtcctcc aaaatatcta caaagtctgc ctttacaaag ctagccaagt
    68401 cttgtggcgc gacgataatg ctgtgtccga cttcgcctgc agagacaatc attcgatcca
    68461 aatctagagc aattttatcg ataaaaatgg gataattgtg tttctgacga attccgacag
    68521 gattattggc tccatgaatg taaccagttg ttttttctaa gtccttttgt ggaatcatgc
    68581 tcactttttt attgccagaa attttagcta gtttcttttc agacaagtgc tgagtgatag
    68641 ggacaattcc gataatcggt ccggtcttgt ctcccaaaag cgccaaggtt ttgaaaatct
    68701 gatctcgttc ataaccttga ggaagctctc cttctagggc attgatttga attccctgat
    68761 gagggatagc tgctttagat aggatttgtt ccaccaatgt ttttttgatt ttaacttttt
    68821 ttgccattat ttatatttat cctccaattg actcatccaa ataccaagcc agattcccag
    68881 cgcaaagaag aaggcgatga tgacataacc gacaagtgaa agtcctgtgt attggatact
    68941 ttcagcgttt cctgcatttg gaattaagat caaaagggta cttgatagga cgataccgat
    69001 gatgaaatga tagacgcgtg agtggtagtt gtttaaggca taatccatca attttgaaaa
    69061 aacgatgaga gttgcacctg caccaattcc aatcggaaag aaggttccca agagatcaaa
    69121 agttttaaaa ccagtcaaca taggagcata gagtcccaaa atcaaaagta aatttgatgg
    69181 gctgaggcca ggaaccaaga cgccaagggc caatagtgcg cctgctagga cgaagttaag
    69241 aaagctggcg cttaaggttc caacgacaaa atttaaggca tagagtccta atccagaaat
    69301 gataaaggtt gtccataacc aagctaaatc aatcttgtct cggtcagatt ctcgagttga
    69361 ttctttgagg aggctaggaa ctgtaccgat aatagctccc gcaaagctcc ataatacaaa
    69421 gacctgataa ttttcaagca ggtattcaat cgggtaggaa aataagccga ttcctagaag
    69481 cataccgatg gcaactggaa taaagtacaa aacattttct ttaaagtctt taaagggatg
    69541 ggccagaaag ccaatcattc gttcatagat tcctaagatt gctgctagaa cccctccgga
    69601 aattcccggt aggataaatc caagagcaat tacaatccct ttaataacgc gtgctaacca
    69661 tgagagcata tatttcctcc aactatttct atttcaaaaa atccttacta tattgtatca
    69721 caatcagaca caaaaagaaa aagcaaatga taaacaaatg cttttaaggt tttaaaaaaa
    69781 gttttcgaaa ggtttcttct ttatttttta agggagagat aacgttgata tctaaatcgt
    69841 ggtcaaagcc ggcaattttt cctttagatg tgtattggtg aatatcataa tctaaatcag
    69901 ttttaggact gctctccaaa aatcctgagt ctgagccgta ggacggaatc caaacagagg
    69961 taaacttgcc tgtatcaata ctgtgttctt ccatgaagta gacaccaacg tagatgccga
    70021 tgtttttagc acctagtgat gctagttttg ctcgaaagtt ttcgacacct tcgttcatat
    70081 tagacatggt tttgtcttcc acgtcaagcc aatagtaact agggctgtaa ggagaggcag
    70141 cattgtagaa aacttcggca gccttttcca tttcttggac actttttcca gctacataag
    70201 cgtagacagc aactgggaca ttccgctttt gaagttcagt gatatgactc ttataggcct
    70261 tgtctattcc attgataaat gaagcatcat tttcttttgt tgtttgagca ccactgtgaa
    70321 cacgaacaat agcacctgaa atattttgtg agagggcatc gtagttgatt tcctcaggac
    70381 gctgccagcc agagaggtca ataatcggtt tgtctaagtg tttcaaagcc tgtgcttcaa
    70441 tctgtgctat attggatttt gttttaaacg attggttgtc attaagtggg cgattgatga
    70501 ttaaaatgaa catcataatc ccaaaaaaac taaataaaat aagtggatga atttgttttc
    70561 tcatatctta taattctacc ctaaaaatca aaaaaaatca aaaaaatggg ttaaggaaga
    70621 gactttagag cattttttca ttcaagagtg cggaatgatt tgaaatatgg tataataaaa
    70681 gggaatttct acagaaaaga gaagattatg tcaaattttg ccattatttt agcagcgggt
    70741 aaagggactc gcatgaaatc tgatttgcca aaagttttgc acaaggttgc gggtatttct
    70801 atgttggaac atgttttccg tagtgtggga gctatccaac ctgaaaagac agtaacagtt
    70861 gtaggacaca aggcagaatt ggttgaggag gtcttggctg gacagacaga atttgtgact
    70921 caatctgaac agttgggaac tggtcatgca gttatgatga cagaacctat cttagaaggt
    70981 ttgtcaggac acaccttggt cattgcagga gatacccctt taatcactgg tgaaagcttg
    71041 aaaaacttga ttgatttcca tatcaatcat aaaaatgtgg ccactatctt gactgctgaa
    71101 acggataatc cttttggcta tggacgaatt gttcgtaatg acaatgctga ggttcttcgt
    71161 attgttgagc agaaggatgc tacagatttt gaaaagcaaa tcaaggaaat caacactgga
    71221 acatacgtct ttgacaacga gcgtttgttt gaggctttga aaaatatcaa taccaataac
    71281 gctcaaggcg aatactatat tacagacgtc attggtattt tccgtgaaac tggtgaaaaa
    71341 gttggcgctt atactttgaa agattttgat gaaagtcttg gggtaaatga ccgtgtggcg
    71401 cttgcgacag ctgagtcagt tatgcgtcgt cgcatcaatc ataaacacat ggtcaacggt
    71461 gttagctttg tcaatccaga agcaacttat atcgatattg atattgagat tgctccggaa
    71521 gttcaaatcg aagccaatgt taccttgaaa gggcaaacga aaattggtgc tgagactgtt
    71581 ttgacaaacg gtacttatgt agtggacagc actatcggag caggagcggt tattaccaat
    71641 tctatgattg aggaaagtag tgttgcagac ggtgtgacag tcggtcctta tgctcacatt
    71701 cgtccaaatt caagtctggg tgcccaagtt catattggta actttgttga ggtgaaagga
    71761 tcttcaatcg gtgagaatac caaggctggt catttgactt atatcggaaa ctgtgaagtg
    71821 ggaagcaacg ttaatttcgg tgctggaact attacagtca actatgacgg caaaaacaaa
    71881 tacaagacag tcattggaga caatgtcttt gttggttcaa attcaaccat tattgcacca
    71941 gtagaacttg gtgacaattc cctcgttggt gctggttcaa ctattactaa agacgtgcca
    72001 gcagatgcta ttgctattgg tcgcggtcgt cagatcaata aagacgaata tgcaacacgt
    72061 cttcctcatc atcctaagaa ccagtaggag cctatcatgg agtttgaaga aaaaacgctt
    72121 agccgaaaag aaatctatca aggaccaata tttaaactgg tccaagatca ggttgaatta
    72181 ccagaaggca agggaactgc ccaacgggat ttgattttcc acaatggggc tgtctgtgtt
    72241 ttagcagtaa cggatgaaca aaaacttatc ttggtcaagc agtaccgcaa agctatcgag
    72301 gcagtctctt acgaaattcc agccggaaaa ttggaggtag gagaaaacac agcccctgtc
    72361 gcagctgccc ttcgtgaatt agaggaagaa acagcctata cagggaaatt agaactcttg
    72421 tacgattttt attcagctat tggcttttgt aatgagaagt taaaactata tttagcaagc
    72481 gatttgacaa aagtggaaaa tccgcgtccg caggatgagg atgaaacctt ggaagtcctt
    72541 gaagtgagct tagaagaagc gaaagaatta atccaatcag gtcatatctg tgatgccaag
    72601 acaattatgg ctgttcagta ttgggagttg cagaaaaaat agaggaggtc agtatgggta
    72661 aatctttatt aacggatgaa atgattgaaa gagctaatag aggcgaaaaa atttcaggtc
    72721 ctcctttgct agatgataat gaggagacta agattttacc aacctcttct tcccgttttg
    72781 gttatgccaa tcctaaggat catggtttta gccaggaaac cttgaaaatt caggtcgaac
    72841 catctattca taaaagccgt cgcattgaaa ataccaagag aaatgtcttc aattctaagt
    72901 tgaataaaat cttattcgcg gtcatctttc tcttgatttt gcttgtttta gcaatgaaac
    72961 ttttgtaata gaaaaggaat tgaaatgaaa ataggaatta ttgctgctat gccagaagaa
    73021 ctggcttatc tggtccagca tttagacaat gcccaggagc aagttgtttt ggggaatacc
    73081 tatcatacag gaaccattgc ttctcatgaa gtcgttcttg tagaaagtgg aattggtaag
    73141 gtcatgtctg ctatgagtgt ggcgattttg gctgatcatt tccaggtgga tgcccttatt
    73201 aatacgggtt cagctggggc agtagcagaa ggtatcgctg ttggggatgt cgtgattgct
    73261 gacaaattag cctatcatga cgtggatgtc acagcttttg gctatgctta tggacaaatg
    73321 gcgcaacaac cgctttattt cgaatcagac aaaacctttg ttgctcaaat ccaagagagt
    73381 ttatctcaat tggaccaaaa ctggcatctt ggtttgattg ctacaggaga tagttttgtt
    73441 gcaggaaatg acaagataga agcgattaag tcccatttcc cagaagtttt agccgtggag
    73501 atggaggggg cagctattgc tcaagcagcg catgccctca atctcccagt cttagtcatc
    73561 cgagctatga gtgacaatgc caaccatgaa gcaaacatct tttttgatga gtttattatc
    73621 gaagctggac gtcgctctgc ccaagtcttg ttgacctttt tgaaggcttt agattaagcg
    73681 gaaatttgac agtttttcta gcttatgata agatttaagt aaagaaaagc tagaaaacgt
    73741 ttcagaggat attatgagta ttgaaatgac cgtcagtgag attgcagagg tcttaggatt
    73801 atctcgccaa gcaatcaata accgtgtcaa agaattacca gaagaagaca cagataaaaa
    73861 tgacaaaggg gtaacagttg ttaccagaag tggcttgatt aagctagaag aaatctataa
    73921 aaaaacgatt tttgaagatg agcctgtcag tgaagatgtc aaacaacgtg aactgatgga
    73981 gattctagtg gatgagaaga atgcagaaat tcttcgtctt tatgaacaat taaaagccaa
    74041 ggatcgtcag ttatcagaaa aagacgagca gatgcgtatc aaagaccgtc agattgctga
    74101 gaaagacaaa caattagatc agcaacaaca attgaccctt caagccatga aggatcaaga
    74161 aaatcttaag ctagagctgg accaagcaaa agaagaagtc caatccacta aaaaaggctt
    74221 ttttgctcgt ttatttggag gataatcaag gagctgttta ggttaaatgc taacctgatg
    74281 gcttcttttg tttgtgaata gtatagttga ttagaacaga gacagtagag gagaggtaca
    74341 gaatggaacg attagaagat tacattgctt ttgatttaga atttaatcag cacgaaggac
    74401 aaacacactt gattcaagta tcggcagtgc gttttagaga tggtcgggaa atcgatgcct
    74461 atgattccta tgttcatacc agcgtccctc taaaaagctt tatcaatggt ttgaccggga
    74521 ttacagctga gaccttaaaa gatgctcctc cagttgagaa agtcttagaa gaattccaag
    74581 agtttgtggg ttctttgctg atgataggct acaatgctgc taaaagtgat ttacctattt
    74641 tagcagagca tggtttagac tatagccaac agtacaaggt ggatttgtat gatgaagctt
    74701 ttgaacgtcg gagttcagat ttacatggta ttgccaatct caaattgcaa actgttgcct
    74761 catttcttgg atttcaaggt cagtctcata atagtttaga ggatgctcgg atgacggcgc
    74821 gtgtttacga atccttctta gagtcggatc aagctaggga attgttagag acagaaagta
    74881 gcttatcaaa caatcctttt ggtggcttgg acttgtctca attatttgac taggagtgcc
    74941 tatgaaacct gaaaaaccta aaaaattggg tcttaaacag atttacctca atcttgataa
    75001 aatcctcttt ctatttttct tgatttttat gatagtggag tatttgtggg taccattcaa
    75061 ttcatggttg gctagcttct tattagatca gacaggttat ctttttattt cctataacaa
    75121 tgtcttagcc atccttactc attctccatt gattagtcta gcttttttag ccttgattgt
    75181 aattaacctt ctggttgctt attttcaaat aggactcctc tttattggtg ctcgtcatct
    75241 cctctataat gaaaagcgaa ggctaattga gtatatccgc aaggttttcc atgaaagttt
    75301 tggatttatg aaaaagctaa cttgcgctaa agccttgttt atctttttct atatagctat
    75361 gctttttcct tttatacgga aaatgttgaa gatttattat cttaataaaa tcgtcattcc
    75421 agagtttatc caagcttatt tagaagataa atattggatg tggtggctga gcatcctact
    75481 tctatctctt atctttctct atgtctctgt cagattgatg tttgcccttc cgaagattgt
    75541 atatgatcag ctgactgttc gagaagcagt gatgtttagc ttggagaaaa ccaagaagag
    75601 agttactttt tatgcttgga atttatttta catcttgctc aaagcacatt tattatttta
    75661 tcttcctttg attcctttat tattggctca aactttggta gatgatatca ctcagagaga
    75721 gtctctgatt cttggtattt tgaattttgt cgtgattaaa aatctctatt atatggctct
    75781 gacttatttt ttggttaaat ttgtttcatt tttgacagga aaggagctgg atatgcttcc
    75841 taggagagaa aaagatcaca tcgtgcgatg gggtgtcatg acttgtgcca gtcttttctt
    75901 tgccttagaa ggttatattt atctggaggc tcccatggtt catctacctc aacttatttc
    75961 tcaccgaggg gtttccaatg caaatgggat tcaaaataca gtagagtcct tggaaactac
    76021 agcacaactc aaaccagact tggtggagac ggacgtgcag gaaacaaaag atgggcagtt
    76081 tgtcatgatg catgatgcta acttgaaaaa tctagcaggt atcaataaaa gtcctcaaga
    76141 cttaaacttg gaggagctta aagggattga tatttttgaa aatggctacc agactaaaat
    76201 ttcaagcttt gaagattatc tcagtcgagc caacgaactt ggtcaaaaat tactaattga
    76261 aattaaaacc agtaaaaaag atagtccaga catgatgaac cgctttttag cccgttatgc
    76321 tgcaaagctc aagatttatg gacatcaaat ccagtcttta gactaccatg ttgtcgaaaa
    76381 agtaagacag tatgatgccg aactgccagt ttatttcatc atgccctaca attctgtctt
    76441 tcctaaaaca agagcgacag gatatactat ggagtactca accttggatg aatattttgt
    76501 aagcaaacta tggacaacgg atcagaaact ttatgtctgg actgtcaatg attcagaagc
    76561 tatcagcaaa tctcttcact taggagtaga tggagtgatt acagatgatt tggaaaaagt
    76621 tcagcgggaa atagaagtag cccaagaaga tccagaatat acggatttgc tcttgaaaaa
    76681 agcattggaa ttctttgaat tttagtaata aaaaagagga acggttgttc ctcccatgat
    76741 aggtaagtat tcttgtgaat gcttgctttt ttgtggttgc aatccgttta cgagaattgt
    76801 ttgtattgtt cttgtttcaa tttactttat agtgctttga agttggaatg ctacactata
    76861 acttataaa
//
LOCUS       DAVKWZ010000003        74211 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000003 DAVKWZ010000000
VERSION     DAVKWZ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 74211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74211
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.003"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            246..1295
                     /locus_tag="VW854_000166"
     CDS             246..1295
                     /locus_tag="VW854_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEV0261475.1"
                     /translation="MLRRNSFLFTATKDGAILRSAKFNYQLNDVSIVIYAFKSGDEFT
                     KEMDNLDVLLSSALGISLSSKEIYATHVEYVFVYRQPERLHVTSLPLEDDSLKIKIYD
                     DFVIDLRKNFSMLISGASGSGKSFFTYYYLTRFISQTVNGKHAKIYVIDPKLSDLYKL
                     CQRSKMPIAHYGTSNEDAFRILRHYIKELDRRMLIYNQSDLFDGIGIDLGFSPLLLVI
                     EEYSSLIASMDSKAKKDFENMVAIVAQKARSLSMGVCIVMQQPRSDSLSTNIREQLVN
                     AIFLGAPTRESSQMMFGTTDVPKVKKDKGVGLYSTDREPPKEFHSPMFDRDVFETIFS
                     IWKFASKDYMKDEDV"
     gene            1473..1736
                     /locus_tag="VW854_000167"
     CDS             1473..1736
                     /locus_tag="VW854_000167"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261476.1"
                     /translation="MKETVVVLAISTKKERGWIKVSTLNDCWSDLGMHFDKSKFGAVF
                     SAPGLYEVEVINNASFGQNPQYEATQCRKIGSFSELVELAKIK"
     gene            1860..2903
                     /locus_tag="VW854_000168"
     CDS             1860..2903
                     /locus_tag="VW854_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEV0261477.1"
                     /translation="MNKEVLNRAPSNSPITIHQMSNKSYSKFQEEVSLKYGFIGLKLD
                     KLSLTAEVSEEFHSEILSGNFTLYDYFGVVEPNLNRNGELASYKGQFFNDEKENWYIE
                     YTPVASIKMNKRPLKIEFTPSKVSKKNFLIVFHRMFPYMFNIAISTFHLAYDFERDLS
                     ALRVNWPKVMYRPIYKGMKLETMDFGAPKGNYHLTAYNKLKERMDSGDMAEIEIYQQY
                     DNLWRIEYKFYNEGNIKKELKNGLPFLAKIPVYIENFKGLEFNNLGVNEKIYLFALRN
                     KPELFAESDKRTVAKYKKLAESISEVNLNVFFQNALDFVEIFNQEPCLINFFEFMNSM
                     LQGDIPKLTINQE"
     gene            2919..3101
                     /locus_tag="VW854_000169"
     CDS             2919..3101
                     /locus_tag="VW854_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEV0261478.1"
                     /translation="MKDTISNKDLISMGYRPSTANAIIHQARELLVSRGYTFYNRKRL
                     MVVPKSVVKELLGMEL"
     gene            3103..4230
                     /locus_tag="VW854_000170"
     CDS             3103..4230
                     /locus_tag="VW854_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV0261479.1"
                     /translation="MAYIEYKQRGKKRLWSFSIRERSKSLLHKSGFKTKREAKIEAEK
                     VLHKLNTGSVLSSSMTLSELYNEWLDLKILPSNRSVVTKKKYLMRKKVIERLFGNKPV
                     SQIKPSEYQKIMNEYGETVSRNFLGRLNSCIRASIQMAIADKVIIEDFTAYVELFSSK
                     SGQKVEEKYLHAESDYQKVLVYLKNKFDYQKSIVPYVIYFLFKTGMRFSELIALTWDE
                     VDELNEQLKTYRRYNTAIHKFTPPKNNTSIRLVPITSDMLSLLKTLKILQLKTNKELN
                     IDNENNLIFQHFGYVYDVPDIATVNKAIKVMLKELQIFPLITTKGARHTYGSYLWHNN
                     IDLGVIAKILGHKDISMLIDVYGHTLEEKISEEFMAVKSLL"
     gene            complement(4313..4385)
                     /locus_tag="VW854_000171"
     tRNA            complement(4313..4385)
                     /locus_tag="VW854_000171"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4350..4352),aa:Lys,seq:ctt)
     gene            complement(4649..5920)
                     /locus_tag="VW854_000172"
     CDS             complement(4649..5920)
                     /locus_tag="VW854_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEV0261480.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYTARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
     gene            6190..6429
                     /locus_tag="VW854_000173"
     CDS             6190..6429
                     /locus_tag="VW854_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261481.1"
                     /translation="MKREVISNGNDGPSQEILIFTKQIRHWILSDQVISGKRKLFFRE
                     DTPKEILDLYENIKSKLDFAYQEVYSNNGLKKYEK"
     gene            6594..6773
                     /locus_tag="VW854_000174"
     CDS             6594..6773
                     /locus_tag="VW854_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261482.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSGPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            6916..7089
                     /locus_tag="VW854_000175"
     CDS             6916..7089
                     /locus_tag="VW854_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEV0261483.1"
                     /translation="MTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERPITILHGELN
                     KYTERGIGKQAGL"
     gene            7126..7578
                     /locus_tag="VW854_000176"
     CDS             7126..7578
                     /locus_tag="VW854_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEV0261484.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            7869..8339
                     /locus_tag="VW854_000177"
     CDS             7869..8339
                     /locus_tag="VW854_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEV0261485.1"
                     /translation="MATYGFLDVLEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNAEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDTLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            8358..9446
                     /locus_tag="VW854_000178"
     CDS             8358..9446
                     /locus_tag="VW854_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEV0261486.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQLKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            9427..9855
                     /locus_tag="VW854_000179"
     CDS             9427..9855
                     /locus_tag="VW854_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV0261487.1"
                     /translation="MELEISDFQGCKIALFCGDKLLTILRDDKDDIPCPNMWELPGGG
                     REGDESPFECARREVYEELGIHLDEDCLLWSKIYPSVIFKGKKSVFMVGQLRQEQFDN
                     IIFGDEGQGYQLMNVEEFLSSSQVVPQLQERLKDYLKVSD"
     gene            9990..10943
                     /locus_tag="VW854_000180"
     CDS             9990..10943
                     /locus_tag="VW854_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000970787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEV0261488.1"
                     /translation="MMETWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNV
                     DRFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARISELTDFMDLGELKIGTTAL
                     AEEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKM
                     SIFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNP
                     GMENIHVAAGDLLKGVEIEADVIVANILADILIHLTEDAYRLVKDEGYLIMSGIIKDK
                     WDMVRELAESAGFFLETHMVQGEWNACVFKKTKDISGVIGG"
     gene            10945..11688
                     /locus_tag="VW854_000181"
     CDS             10945..11688
                     /locus_tag="VW854_000181"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEV0261489.1"
                     /translation="MQQYFVKGSAISPVTIENKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLTQLDQ
                     FDSIIVAYEESGKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     VGLGPRILRAETAPLYALSALSVLVELEK"
     gene            11699..13495
                     /gene="pepF"
                     /locus_tag="VW854_000182"
     CDS             11699..13495
                     /gene="pepF"
                     /locus_tag="VW854_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV0261490.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVVADEEVLDRLGELPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKNSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            13742..14296
                     /locus_tag="VW854_000183"
     CDS             13742..14296
                     /locus_tag="VW854_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEV0261491.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLFDHLGNPSPEIRDDLSNQGL
                     HYLSKEKDTTDFSSQYGWVHAFAHGADLLTEVVCHPDFPINRIHEVFDILGQLFKRMS
                     IRFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVDFPIEEREDFYKFSNFRSCLVE
                     VYVQLDQRNSLQDDLKEAIQSFQY"
     gene            14407..15075
                     /locus_tag="VW854_000184"
     CDS             14407..15075
                     /locus_tag="VW854_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEV0261492.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLGN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
     gene            complement(15379..15468)
                     /locus_tag="VW854_000185"
                     /pseudo
     CDS             complement(15379..15468)
                     /locus_tag="VW854_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(15465..15998)
                     /gene="queF"
                     /locus_tag="VW854_000186"
     CDS             complement(15465..15998)
                     /gene="queF"
                     /locus_tag="VW854_000186"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEV0261493.1"
                     /translation="MKRQSALVVFSGGQDSTTCLFWTMQHYETVEAVTFAYGQRHHLE
                     IQIAREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
     gene            complement(16304..16618)
                     /gene="trxA"
                     /locus_tag="VW854_000187"
     CDS             complement(16304..16618)
                     /gene="trxA"
                     /locus_tag="VW854_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV0261494.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            complement(16635..16856)
                     /locus_tag="VW854_000188"
     CDS             complement(16635..16856)
                     /locus_tag="VW854_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV0261495.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            complement(16955..17335)
                     /locus_tag="VW854_000189"
     CDS             complement(16955..17335)
                     /locus_tag="VW854_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV0261496.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            complement(17479..18432)
                     /locus_tag="VW854_000190"
     CDS             complement(17479..18432)
                     /locus_tag="VW854_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV0261497.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIESYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYGQ"
     gene            complement(18500..19708)
                     /gene="sstT"
                     /locus_tag="VW854_000191"
                     /pseudo
     CDS             complement(18500..19708)
                     /gene="sstT"
                     /locus_tag="VW854_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            19847..20407
                     /locus_tag="VW854_000192"
     CDS             19847..20407
                     /locus_tag="VW854_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEV0261498.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            20464..21372
                     /locus_tag="VW854_000193"
     CDS             20464..21372
                     /locus_tag="VW854_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV0261499.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            21324..21914
                     /locus_tag="VW854_000194"
     CDS             21324..21914
                     /locus_tag="VW854_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEV0261500.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            21917..23023
                     /gene="yqeH"
                     /locus_tag="VW854_000195"
     CDS             21917..23023
                     /gene="yqeH"
                     /locus_tag="VW854_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEV0261501.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGIAKVAVWAPEGVAVVTRKAII"
     gene            23237..23548
                     /gene="yhbY"
                     /locus_tag="VW854_000196"
     CDS             23237..23548
                     /gene="yhbY"
                     /locus_tag="VW854_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEV0261502.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            23600..24229
                     /locus_tag="VW854_000197"
     CDS             23600..24229
                     /locus_tag="VW854_000197"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEV0261503.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKV
                     GTSYPVIWVDVPLMDISSSMVRDFLAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            24229..24822
                     /gene="yqeK"
                     /locus_tag="VW854_000198"
     CDS             24229..24822
                     /gene="yqeK"
                     /locus_tag="VW854_000198"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEV0261504.1"
                     /translation="MSYQDYINCSREALLEKMAEFLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSNQEFLALIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIANLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKEN"
     gene            24823..25323
                     /locus_tag="VW854_000199"
     CDS             24823..25323
                     /locus_tag="VW854_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV0261505.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLGKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            25336..25689
                     /gene="rsfS"
                     /locus_tag="VW854_000200"
     CDS             25336..25689
                     /gene="rsfS"
                     /locus_tag="VW854_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEV0261506.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            25792..26538
                     /locus_tag="VW854_000201"
     CDS             25792..26538
                     /locus_tag="VW854_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV0261507.1"
                     /translation="MIMATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLE
                     LACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGK
                     YDFVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHE
                     NAEDFAMLWDTYEDEVPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQ
                     AGFKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            26548..27645
                     /locus_tag="VW854_000202"
     CDS             26548..27645
                     /locus_tag="VW854_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEV0261508.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGSVDILDRLGIDSLAFGTEEVLD
                     YQKIADLYTEKGAEMEKFVKNLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILMQARESDLPEAIHVLGFTEKGRQHLKSLKGQVSLVSRIGKEPWDA
                     MTQKVDQIYQLGKPSIAEQNFGRVPIRIETN"
     gene            27883..28137
                     /locus_tag="VW854_000203"
     CDS             27883..28137
                     /locus_tag="VW854_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEV0261509.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMAQVRAGSTQVHVIEE"
     gene            28141..28395
                     /locus_tag="VW854_000204"
     CDS             28141..28395
                     /locus_tag="VW854_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEV0261510.1"
                     /translation="MLLKFTEDAWADYCYWQNQDKKTLKRINKLIKDIQRDPFTGIGK
                     PEPLKYDYQGAWSRRIDAENRLIYMMDGDSVAFLSFKDHY"
     gene            28586..30199
                     /locus_tag="VW854_000205"
     CDS             28586..30199
                     /locus_tag="VW854_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEV0261511.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            30329..30955
                     /gene="gmk"
                     /locus_tag="VW854_000206"
     CDS             30329..30955
                     /gene="gmk"
                     /locus_tag="VW854_000206"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEV0261512.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            30980..31294
                     /locus_tag="VW854_000207"
     CDS             30980..31294
                     /locus_tag="VW854_000207"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEV0261513.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            31360..33756
                     /locus_tag="VW854_000208"
     CDS             31360..33756
                     /locus_tag="VW854_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEV0261514.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            33769..34704
                     /locus_tag="VW854_000209"
     CDS             33769..34704
                     /locus_tag="VW854_000209"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEV0261515.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEELMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            34697..36010
                     /gene="rsmB"
                     /locus_tag="VW854_000210"
     CDS             34697..36010
                     /gene="rsmB"
                     /locus_tag="VW854_000210"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEV0261516.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLLAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNALLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANDSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            36025..36765
                     /locus_tag="VW854_000211"
     CDS             36025..36765
                     /locus_tag="VW854_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEV0261517.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDAE"
     gene            36762..38741
                     /gene="pknB"
                     /locus_tag="VW854_000212"
     CDS             36762..38741
                     /gene="pknB"
                     /locus_tag="VW854_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEV0261518.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            38856..39614
                     /locus_tag="VW854_000213"
     CDS             38856..39614
                     /locus_tag="VW854_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV0261519.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            39616..40065
                     /locus_tag="VW854_000214"
     CDS             39616..40065
                     /locus_tag="VW854_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV0261520.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            40127..40753
                     /locus_tag="VW854_000215"
                     /pseudo
     CDS             40127..40753
                     /locus_tag="VW854_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            40821..41069
                     /locus_tag="VW854_000216"
     CDS             40821..41069
                     /locus_tag="VW854_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261521.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRTYSGNLILQELITVNGAYKATYIDELSSN"
     gene            41200..42372
                     /locus_tag="VW854_000217"
     CDS             41200..42372
                     /locus_tag="VW854_000217"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEV0261522.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIVLYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            42372..43646
                     /locus_tag="VW854_000218"
     CDS             42372..43646
                     /locus_tag="VW854_000218"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEV0261523.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(43682..44647)
                     /locus_tag="VW854_000219"
     CDS             complement(43682..44647)
                     /locus_tag="VW854_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV0261524.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(44628..46082)
                     /locus_tag="VW854_000220"
     CDS             complement(44628..46082)
                     /locus_tag="VW854_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV0261525.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLIHFRETGVKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDKNWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFQGQYYAIVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            46458..48341
                     /locus_tag="VW854_000221"
     CDS             46458..48341
                     /locus_tag="VW854_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV0261526.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSIPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     AQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTADYASVAPVATGSVAKGDAVIE
                     VKI"
     gene            48490..49377
                     /locus_tag="VW854_000222"
     CDS             48490..49377
                     /locus_tag="VW854_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV0261527.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEVVARNNAGGRIENLVYYTIGTGIGAGVLQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIEFNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(49697..50314)
                     /locus_tag="VW854_000223"
     CDS             complement(49697..50314)
                     /locus_tag="VW854_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEV0261528.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(50729..50942)
                     /locus_tag="VW854_000224"
                     /pseudo
     CDS             complement(50729..50942)
                     /locus_tag="VW854_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(51427..51507)
                     /locus_tag="VW854_000225"
                     /pseudo
     CDS             complement(51427..51507)
                     /locus_tag="VW854_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            51623..52516
                     /locus_tag="VW854_000226"
     CDS             51623..52516
                     /locus_tag="VW854_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0261529.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLKVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            52513..53712
                     /locus_tag="VW854_000227"
     CDS             52513..53712
                     /locus_tag="VW854_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0261530.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHIQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINDYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(54125..54892)
                     /locus_tag="VW854_000228"
     CDS             complement(54125..54892)
                     /locus_tag="VW854_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261531.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAVYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVEELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(54909..55604)
                     /locus_tag="VW854_000229"
     CDS             complement(54909..55604)
                     /locus_tag="VW854_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0261532.1"
                     /translation="MSLLAFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            complement(55609..55974)
                     /locus_tag="VW854_000230"
     CDS             complement(55609..55974)
                     /locus_tag="VW854_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV0261533.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            56114..56587
                     /gene="nrdR"
                     /locus_tag="VW854_000231"
     CDS             56114..56587
                     /gene="nrdR"
                     /locus_tag="VW854_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEV0261534.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            56588..57757
                     /locus_tag="VW854_000232"
     CDS             56588..57757
                     /locus_tag="VW854_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEV0261535.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLGHPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKETTIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEKAVLRIRER
                     EQKAKTQKQNQTAPAKTNIPKWSNPEYKNTSSAEELEEMERQTLELLAKLDNGGD"
     gene            57758..58654
                     /gene="dnaI"
                     /locus_tag="VW854_000233"
     CDS             57758..58654
                     /gene="dnaI"
                     /locus_tag="VW854_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEV0261536.1"
                     /translation="MESVGDVLKRQPSRFHYQDLVQKIMKDPDVAAFVQQESLNQDEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVMNHGYADVSYEETPELIAAEKEAA
                     IKKRLNLINFPSSLKNVSFLDVYRDDVQRLTVLKRMIEFVNDYPNNLKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSDVLILD
                     DIGAEQSTVWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            58651..59364
                     /locus_tag="VW854_000234"
     CDS             58651..59364
                     /locus_tag="VW854_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEV0261537.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            59378..60688
                     /gene="der"
                     /locus_tag="VW854_000235"
     CDS             59378..60688
                     /gene="der"
                     /locus_tag="VW854_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEV0261538.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            60779..61114
                     /locus_tag="VW854_000236"
     CDS             60779..61114
                     /locus_tag="VW854_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261539.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            61255..61600
                     /locus_tag="VW854_000237"
                     /pseudo
     CDS             61255..61600
                     /locus_tag="VW854_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            61785..62576
                     /locus_tag="VW854_000238"
     CDS             61785..62576
                     /locus_tag="VW854_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261540.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLTIAIITYFLGTFSPLGWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYIVPYMVLEKYEDNV"
     gene            62569..63210
                     /locus_tag="VW854_000239"
     CDS             62569..63210
                     /locus_tag="VW854_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261541.1"
                     /translation="MFKNFNNIWLKRKIVLLLRIVLMMILINYLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLLLLSLLVMLIVSRLEEKFKKG"
     gene            63214..63846
                     /locus_tag="VW854_000240"
     CDS             63214..63846
                     /locus_tag="VW854_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV0261542.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKILAGYIKLDKGKVLQDGKVYGVKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDIEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQM"
     gene            63874..64161
                     /locus_tag="VW854_000241"
     CDS             63874..64161
                     /locus_tag="VW854_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261543.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWIAFFFLPWGSPILGGEIDLFS
                     IQKVLLVFGILSILMALLSKKVSLFVFGLICCLSLWINLFILFAILPILGN"
     gene            64307..66820
                     /gene="secA"
                     /locus_tag="VW854_000242"
     CDS             64307..66820
                     /gene="secA"
                     /locus_tag="VW854_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEV0261544.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYTQRYDVITADRDL
                     APEIQAMIKRTIGRVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            66901..67932
                     /locus_tag="VW854_000243"
     CDS             66901..67932
                     /locus_tag="VW854_000243"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV0261545.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            67934..68965
                     /locus_tag="VW854_000244"
     CDS             67934..68965
                     /locus_tag="VW854_000244"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV0261546.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            69004..69372
                     /locus_tag="VW854_000245"
     CDS             69004..69372
                     /locus_tag="VW854_000245"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEV0261547.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVIASVILEENHES"
     gene            69362..70465
                     /locus_tag="VW854_000246"
     CDS             69362..70465
                     /locus_tag="VW854_000246"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEV0261548.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVNGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVRVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHIKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKPYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            70484..72499
                     /gene="recG"
                     /locus_tag="VW854_000247"
     CDS             70484..72499
                     /gene="recG"
                     /locus_tag="VW854_000247"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEV0261549.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQTLKSENRVQGSGLVLDWSQEKVTAVKASLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            72815..73795
                     /locus_tag="VW854_000248"
     CDS             72815..73795
                     /locus_tag="VW854_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEV0261550.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHETMNVFVNDQVYNWLCGSEIPFKYLK"
     gene            complement(74117..>74211)
                     /locus_tag="VW854_000249"
     CDS             complement(74117..>74211)
                     /locus_tag="VW854_000249"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="lysyl-tRNA synthetase"
                     /protein_id="HEV0261551.1"
                     /translation="FEVVDRTDEVSNHTYGKAKLTWFEEIFEKY"
BASE COUNT        22004 a        14004 c        16838 g        21365 t
ORIGIN      
        1 aacaaacggc ttctttgtct gatcattatt ttttaaatac atctaaattg agaagtattc
       61 gctggtttac tataggattt ttgagcatat tgtccctttc tagtagtatt ctttttaagg
      121 gatggtttct tcaaatgatt acattatccg ttggattgat tataacttcg tatattgaaa
      181 gaaaaatcaa gggatgggct cagcagattg aaccgctgtt aattgtcaga gaaaaccttc
      241 tatttatgct tcgcagaaat tcctttcttt ttacagcaac aaaagatggc gctattcttc
      301 gttcagctaa atttaattac caattgaatg atgtttcaat tgtaatctat gcatttaagt
      361 ctggggatga attcacgaaa gagatggaca atttagacgt tctcctaagt agtgctctag
      421 gaataagcct atcgtcaaaa gaaatttatg cgactcatgt tgaatatgtt tttgtatata
      481 gacagcctga acgtttacat gttacctccc tccctcttga agatgatagc ctgaaaatta
      541 aaatctatga tgattttgtt atcgatttga gaaaaaactt ttccatgctt atatcgggtg
      601 cctccggtag tggtaaatct ttttttacat attattactt gactcgtttc atatctcaga
      661 ccgtaaatgg gaaacatgca aaaatatatg tgattgaccc aaagctgtct gacctttata
      721 agctttgtca gcgttctaag atgccgatag cccattatgg aacgagtaat gaggatgcct
      781 ttagaattct gcgtcactat atcaaggagc tagatagacg tatgctaatc tacaatcaaa
      841 gtgacttgtt cgatggtatt ggcatagact taggtttttc tcctttactt ctggtaatag
      901 aggagtattc atcgttaatt gcttcaatgg atagtaaagc taaaaaagat tttgaaaata
      961 tggttgctat cgttgctcaa aaagcaaggt ctttatctat gggggtctgc attgttatgc
     1021 aacaaccccg ttccgatagc ctttcaacaa atattcgtga acaattggtc aatgctatat
     1081 ttcttggagc cccaactcgc gaatctagcc agatgatgtt tggcacaaca gatgtaccca
     1141 aagttaagaa ggataaagga gttgggcttt attctacaga tagagagcct cctaaggaat
     1201 tccattcccc gatgtttgat agagatgtgt ttgagacaat tttttcaatt tggaaatttg
     1261 cttctaaaga ttatatgaag gatgaagatg tatgagaatt ttaagcgttt tgaagatgaa
     1321 taaaattctt ttattcattt tcaaaatgcc attgtgcagc cggcgaacaa caaggccggt
     1381 cggacgtaac gtccgaataa atgtatttat gtccaatact gattgacgtt aaaagctgta
     1441 tattatttta aataaggaag gattttttaa caatgaaaga aactgttgtt gttctagcta
     1501 tttctactaa gaaagagcga ggctggatca aggtctccac ccttaatgat tgttggagtg
     1561 acctaggtat gcatttcgat aaatccaaat ttggagctgt cttctctgct ccaggccttt
     1621 atgaggttga agtgattaat aatgcttcat ttggacagaa tccccaatat gaggcgactc
     1681 aatgtagaaa aatcgggagt ttttccgaat tagttgaact ggcaaaaatt aaataattta
     1741 tatttgggta aggctggacc tgccagcttt gctggcaagt ggagcgtggt cagtggaacg
     1801 gcagtcttgc cctaaaattt aagtatatag aatgttttgt tttttgaagg agaaatttca
     1861 tgaataaaga agttttaaat cgggcccctt ctaatagccc gataacgata catcaaatgt
     1921 ctaataagag ttactctaaa tttcaagagg aagtatctct gaagtatggg tttatcggtc
     1981 tgaaattgga taagctaagt ttgaccgctg aagttagtga ggaatttcat tctgagattc
     2041 ttagtgggaa cttcacctta tatgattatt tcggtgtagt tgagccgaat ttaaatagaa
     2101 atggtgaact agctagttat aaagggcaat tctttaatga tgaaaaagag aattggtata
     2161 tcgagtatac ccccgttgcg tcgattaaga tgaacaaaag acctttaaag attgaattta
     2221 ctccttcaaa agtttcaaaa aagaattttt taattgtctt tcatagaatg tttccgtaca
     2281 tgtttaacat cgcaatctct acttttcatt tagcctatga ttttgagcga gatttatcgg
     2341 ctttacgtgt aaattggcca aaagtaatgt atcgtcctat ttataagggg atgaaacttg
     2401 agactatgga ttttggtgct cctaaaggta attatcatct aactgcttat aataaactta
     2461 aagaacgtat ggatagtgga gatatggcag aaatagaaat ttatcagcaa tatgataatc
     2521 tatggaggat tgagtataaa ttctacaatg aaggcaacat aaaaaaagaa ttaaaaaatg
     2581 gattaccatt cttagctaaa attccagtgt atattgaaaa tttcaaaggc ttagaattta
     2641 ataatttagg agttaatgag aagatttatc ttttcgctct tcgtaataaa cctgaattat
     2701 ttgcagaatc tgataaacga acggtagcta agtataaaaa acttgccgaa agtatctctg
     2761 aagttaattt gaatgtcttt tttcaaaatg ctttagattt tgttgaaatc tttaatcaag
     2821 agccttgtct gataaatttt tttgagttta tgaattctat gttacaaggc gatattccaa
     2881 agttaacaat aaaccaagaa taataagagg ggaacagtat gaaagatacg atttctaata
     2941 aggatttgat ttccatgggc tatagaccat caacagcaaa tgctattatc catcaggcga
     3001 gagaattact tgtatcacga ggctatacat tttataatcg caaacgtttg atggttgttc
     3061 caaaaagtgt tgtgaaagag ttgttgggaa tggaattgtg aaatggctta tatcgagtat
     3121 aaacagcgtg ggaagaaaag gctttggtcc ttttctataa gagaaagaag caagagccta
     3181 ctccataaaa gcggatttaa aacaaaacga gaagctaaaa tagaagcgga gaaagttctt
     3241 cataagttaa atactgggag tgtcttaagc tctagtatga ctttatcgga gctttataat
     3301 gaatggcttg atttaaaaat tttacctagc aatagaagtg tagttactaa aaaaaaatat
     3361 ctcatgagaa agaaggtcat cgaaaggtta tttggaaata agcctgtatc acaaattaag
     3421 cctagcgagt atcaaaaaat tatgaatgag tatggagaga ctgtatcgag aaatttctta
     3481 ggaagattga attcttgtat tcgggcaagt atacagatgg ctattgctga taaggtgata
     3541 atagaggatt ttactgctta tgttgagttg ttctcttcta agagtggaca aaaggttgag
     3601 gaaaagtatt tgcatgctga gtcagattat caaaaagttt tagtatattt gaaaaataag
     3661 tttgattatc aaaaatctat tgtaccgtat gtaatttatt tcctttttaa aactggcatg
     3721 cgtttctccg aattgattgc tctaacttgg gatgaagttg acgagcttaa tgagcaactt
     3781 aaaacttatc gtcgttacaa cactgctatt cataagttta ctccacctaa aaacaatact
     3841 tcaataagat tagttcctat tacttcagat atgctttcct tgttaaaaac attgaaaata
     3901 ttgcaattaa aaacaaataa agaattaaat attgataatg aaaataattt gatttttcag
     3961 cattttggct atgtctacga tgttccagat attgcaacag tcaataaagc tataaaagta
     4021 atgttgaagg aattgcaaat ttttccattg attactacca aaggagctag acacacttat
     4081 ggtagttatt tatggcataa taatattgat ttgggagtta tagcgaaaat tttaggacat
     4141 aaagatatct ccatgttgat tgatgtttat gggcatacct tggaggaaaa aattagtgag
     4201 gagtttatgg ctgtgaaaag tttgttgtaa aaacacggca aattgcacga caatttttaa
     4261 aaggaaagca aaaaaagccc tatattataa ggcttttcct gttgtattta tatgccccct
     4321 acagggatcg aacctgtgac ccacggatta agagtccgct gctctgccag ctgagctaag
     4381 gaggcaaaga aaaagctgta ttggtgccga aacttcacgg tttgtattga acccgcgcaa
     4441 ttaagcaggt gggcaactcg ctctaactga agctgtttcc gtgtgagacg gcctacatgc
     4501 tgttagaaga cttttgtttc cctaataata caaaaaatag tcggtcaaca cttaagtgtg
     4561 aagtcgtaca ccacagcgtt tctatactta tatgatacca ctttttcaaa aaaaatcaag
     4621 aggaaagtgc aattttttga aaaggatttc agatttttcg caaacgatcg atagcttcct
     4681 ttctttgagc caaagcccgt tcatatttac cagtatcttc tgcttggaaa tagtgatgat
     4741 tacgaatttt ttctggcaga tagtcttgct tgacccaatt tccaggatag ttgtgtggat
     4801 agagatagtc ttgggcattc cccagttcct tgcttccact gtagtgccca tcacgcaggt
     4861 gtcgcggaat aggcaagtgc cctgatgttt tgaggtcagc aagtgcctta tccatagcta
     4921 cataggctga gttggatttt ggagaaaggg ccaaatcaat cacgacattg gcaatgagaa
     4981 tgcgggcttc ggggaaacca atcctttgtg cagcatccag agcagtcacg gtatgaatct
     5041 gggcctcagg attggctaag ccgatatctt cataggcgat aacagtcaag cgacgagcga
     5101 gactaggcag atccccagcc tcaatcaagc gggcagtata gtggagactg gcatccacat
     5161 ctgagccacg aatagacttt tgcagggcag atagaacatc atagtgaccg tctccatcct
     5221 tgtccatagt gatgtagctt ctctgaagac tattttccat gatgtctaag gtgatgtggc
     5281 gaatgccctc gtcattctca ggggtagata gaacagccaa gtccagtgag ttaaaggcag
     5341 agcgaaggtc tccgtttgta gaggttgcga tgaaatccag cgcatcctca tctagttcta
     5401 ttggaaaatc aaaaccacgt tcagggttac ttagagctat ctgcagggcc tctttgacgt
     5461 cttggttaga cagaggttcc aactcgaaaa tttgaactcg gctacgaatg gcaggagtga
     5521 cagagaagaa aggattttca gtcgttgctc caatcatgat gaccagtcca ctttccaaga
     5581 gaggcaagag gaagtcttgc ttggtcttat ctagtcgatg aatttcgtct agcaatagga
     5641 cgagaccacc agaaaattta gcttcttccg agatttcttg cagtcgcttt ttactatcaa
     5701 ctgtcgcatt gaaagttcga aaggcatact tggtcgttcc agcgatggca gaggcaatac
     5761 tggttttgcc gattccagga gggccatata gaatcatgga ggacaggcgg ttggcttcca
     5821 ccatgcggcg gatgattttt ccaggtccga ccagatgctc ctgaccgatg acctggtcga
     5881 tggttttagg gcgcatgcga agcgcgagat tgtctggcat agcagtcctt tctaacgtgg
     5941 atttgagtag aatgactcat ctactatatc ttactatgtc cattctagcc tattagttgt
     6001 gatatttcaa tggttttgta ataatcaggg agtgagatag aatctttttt actatttaat
     6061 ttaatgagga gaggtatcag catgagagat ttgattttcc ttaaagctca aaataggata
     6121 gttgagccta aagaagtgga agtgctgact tgagaaataa tattgctgaa tgtcattgta
     6181 tgaactataa tgaaaagaga ggtgataagc aatggaaatg atggaccaag tcaggaaatt
     6241 ttgattttta caaaacaaat tcgtcattgg attcttagtg atcaagttat ttcagggaaa
     6301 agaaaacttt tcttccggga agatactcca aaagagattt tagatttgta cgaaaacatt
     6361 aaatctaaac ttgattttgc ttaccaagaa gtttattcta ataatgggtt gaaaaaatat
     6421 gaaaaataga ataattgatg tttttgaagt agtaaatcgc atccttgtaa taactgttga
     6481 aaatcctaat ttcgaagact tgagagttaa tcatactcaa tgaaaatcaa agagcaaact
     6541 aggaaactag ccgcaggctg tacttgagta cggtaaggcg acgttgacgt ggtttgaatt
     6601 tgattttcga agagtatcag tttgtaaaaa ttggagataa aaaatataga gtacgcagtg
     6661 gtcctatgat tcattcaact ccgcctcaat ctgtcttaga tcgagatact tttacaattg
     6721 actatacaga tgatgaattg ctcgataaag aagcagtttt tactactcat tagaaaaata
     6781 ggtctgaata aattggtttc ccttagaacc acatctaggg gcttttatgt ataatagaat
     6841 aataagtatc actcctttac aatacgtgta acacgtgtta taatggtgtt gtcaggaggt
     6901 aaaagcgcta tacctatgac acaaaaagag atggttaaac tccttacggc ccatggttgg
     6961 ataaagacta gaggcggtaa aggctcccat attaaaatgg agaagcaagg ggaaagacct
     7021 atcacaatcc ttcatggtga gctgaataag tacactgaaa gagggatagg aaagcaagct
     7081 gggttgtaaa attcagtccc tgctttttga tatggaggta gtgaaatgtt agttacgtat
     7141 ccagctttat tttattatga cgacacagac ggaacagaag cgacttattt tgtccatttc
     7201 ccagattttg aatactcagc tacacaagga gaggggattt ctgaggcttt ggctatgggg
     7261 tcggagtggc tagggataac tgttgcagat ttgatagaga gtgatggaga gttacctcag
     7321 ccatcagata tcaatagctt gtctttaatt gataatgatc cttttaaaga tgatgaagat
     7381 ttcgtgtcaa cctatgacct tgataaatct tttatttcta tggtatctgt tgatgtatca
     7441 gaatacctag gaagtcagga acccattaaa aagactttga ccataccaaa atgggcagat
     7501 aagttgggac gagaaatggg acttaacttt tctcagactt tgacagacgc tattgcagat
     7561 aagaaagttc aagcctaaga ttaagcgtgg tatcgtggta atagtcaata aaaaagcacg
     7621 tccacttgtg ctagttactc gcctattgat tttatagatt tattagccct ttcgcgggct
     7681 ttttatattg atttttatga gaaatgaaga aatcagactt ttaagaaaat cactgatacc
     7741 aagggtttaa atgaggtaat atggtataat taggacataa aataattttg tggtaagatg
     7801 gtagtatcta ttttagcata tttccgagca atcggggcga ttaaagagtc gcatagaaag
     7861 aggacaaaat ggcaacatac ggatttttag atgttttaga ggaagagttg gacaagaact
     7921 ttccctttga ctttgagatt agctgggaca aacgcaacca tgcggtagaa gtgagttttc
     7981 tattggaagc acaaaatgct gcaggtgtgg agatggtgga tgaagacgga gaggtttcat
     8041 cagatgacat tctttttgag gaagcggtcc ttttctacaa tcctgctaag tcaaccgtca
     8101 atgcagaaga ctatttaacg gttatccctt atctacctaa aaaaggattt tctcgcgagt
     8161 ttttagctta ttttgccctt ttcctcaaag atactgcgga agttgggcta gataccctca
     8221 tggacttttt ggaagatcca gaagcagagg aatttgttat ggaatggaat caagaagtct
     8281 ttgaagaagg aaaagttggc ttggaagagg gagaatttta tccttatcca agatactagg
     8341 agttggttgg aggtgttatg aagaaaattg ggacttattt ggtttatgtg ctagctgttg
     8401 tctttattat gctagctttt gcttgtggaa caatcgcatt tgcagagttg gggtattccg
     8461 cagttttagc ctttactttt ggttatgcct tcgctcttct aagcatgtat ttaatcttta
     8521 ttcttcatga gctgggtcat gctttttgtg gctacttgac aggctatcgg ctggtggctt
     8581 ttggattagg acattttatt ttgaccaaaa agtcaggcag gtttcatctt agtagaacag
     8641 ccattctgaa aaatgttggt gctcaataca ttggtttaaa agaggatgaa agcgatcaaa
     8701 gaatcatcct gatgctttca ggaggcttga tggttcatct cggcttgata ttattggcga
     8761 tagtgtttgg atttttgaca agaagctggt attttgcagg gacttggatt tttcttaatt
     8821 tatctttctt cctaaataac attttgccag tcgacatcac cgatggagca aaaatttggg
     8881 aattactaca acaccctgaa aataccaaat acgcctacct ggtattgaga cattctgctc
     8941 aaactttgct ggctcctcaa gaatatgatt tgaaagactt tgttcaagca gttcctgagg
     9001 atgcacgagg gagttttgcg gatggtgtat taggtatgca aggtgaagtt tccattttgg
     9061 aagggaagga agaagtagct aaacagcagt ttcaagcatt acttgagagg acaactactc
     9121 cgatgatgca gacagttgcc caactatccc tgcttcatat cgccttgttg gaaggagatt
     9181 ttgagcaagc ggagcaatat gctagtattc gacaggtcaa atcgtttctg tctctcaaac
     9241 tgtccaatct tcaagtgata caagcctggt atcaattaaa ggtgaagaaa gatgtagatc
     9301 gaactcgtaa ggctctcaag attgctagac agaaaatgga tagtagtcga atgttgaggg
     9361 acgagaaacg ctattatgaa aacttgttag cagagctgga aaaggaaatg ttagaaggaa
     9421 ctgaagatgg aactagaaat ttctgatttc caaggttgca agattgccct cttttgtggg
     9481 gataagctac tgacgatttt gcgtgatgac aaggacgata tcccttgtcc caacatgtgg
     9541 gagttgccag gtggtggccg tgaaggggac gaaagccctt ttgaatgcgc tcggcgtgaa
     9601 gtttatgagg aactgggaat tcatttagat gaagactgtc tgctttggag caagatttat
     9661 cctagcgtaa tttttaaagg caagaaatcg gtctttatgg ttggtcagct aagacaagaa
     9721 caatttgaca atatcatctt cggagatgaa ggacagggct atcagctgat gaatgttgag
     9781 gaatttctta gttccagtca agttgtgcct cagttgcaag agagattaaa agattattta
     9841 aaagtaagtg attagaaagg atggttcagt taaatttcta aactgaaccc gccctaaaca
     9901 ctgtgccaaa aagataaact tctcttagac acaagcgtct tcagagaatt tcctattttg
     9961 gttttgtgtt ttacgggctt ggtatcttaa tgatggaaac atggcaagag ttaaaagtta
    10021 cagtgaagcg tgagggagag gagttggttt ccaatctctt gattgagctg ggagcgcaag
    10081 gtgttgcgat tgaagacagt atggactatg tgggaaatgt tgaccgcttc ggcgagattt
    10141 tcccagaggt cgagcagcaa gaagaaatcg tagtgacagc ctactaccct gacacggtgg
    10201 atgtgacagt ggttgaggcg gacttgcagg ctcgcatatc agaattgacg gattttatgg
    10261 atttgggaga gttaaaaata ggtacgactg ccttggctga ggaagactgg gcagataact
    10321 ggaagaaata ctatgagcca gctcgtatca ctcatgattt gaccatcgtt ccctcttgga
    10381 cagactatga ggcgactgcg ggagaaaaaa ttatcaagct ggatcctggc atggcttttg
    10441 gtacgggaac ccacccaact accaagatga gcatttttgc cttggaacag gttcttcgtg
    10501 gtggcgaaac ggtgctagat gtgggaactg gttcaggggt tctctctatt gccagctcgc
    10561 ttctgggggc taaagaaatt ttcgcctatg acctggatga tgtggcagtt cgtgtggctc
    10621 aggaaaatat tgagctcaac cctggaatgg aaaatatcca tgtagcggca ggcgatttgc
    10681 ttaaaggcgt ggagattgaa gcagatgtca tcgtggcaaa catcttggcg gatatcctca
    10741 ttcatctgac agaggatgct tatcgcttgg ttaaggatga aggttacctg ataatgagtg
    10801 gcattatcaa ggataagtgg gacatggtgc gcgagttggc tgaatcagct ggatttttcc
    10861 tcgaaactca catggttcaa ggggaatgga atgcctgtgt ctttaagaaa accaaggata
    10921 tctctggtgt gattggaggc tagcatgcag cagtattttg taaaaggcag tgctatctct
    10981 cctgtcacta tcgagaacaa ggaaaccagt aagcatatgt ttcaggttat gcgcttgaaa
    11041 gaagaggatg aggttacctt agtctttgat gatggcatca agcgcttggc gcgcgtgctg
    11101 gatatggaaa atcgtcagtt tgagttggtc caagaattaa atgacaatgt ggaactacca
    11161 gttcaagtga ccatcgcatc tggatttccc aagggagaca agctggagtt catcgctcaa
    11221 aaagtaaccg aactgggtgc tagccaaatc tgggcctttc ctgcagactg gtcagttgcc
    11281 aagtgggatg gcaagaaatt gggtaaaaaa gttgaaaaac tagaaaaaat tgcccttgga
    11341 gcagccgagc aaagcaagcg taatattgta ccaagtatta aacttttcga gaaaaaagca
    11401 gattttctaa ctcagctgga ccagtttgac tctatcatag tagcctatga agaatcaggt
    11461 aaagaaggag aagccgctgc gcttctgcaa gcagtcactg gtcttgaaac aggagccaaa
    11521 ctgctcttta tctttggtcc agaaggcggt ctctcacctg cagaaatcga aagttttgaa
    11581 gctaagggag ccgttttggt aggcctaggt cctcgtattt tgcgagcaga aacagcaccg
    11641 ctttacgcct tatcagccct tagtgtttta gtagaattag agaaataaga ggaagaaaat
    11701 ggaacaaaaa caccgttcag aatttccaga gaaggaactc tgggacttaa cagccctata
    11761 ccaagaccgt gaggatttct tgcgtgcaat cgagaaagct cgtgaagaca tcaaccaatt
    11821 tagccgtgac tacaagggta atcttcatac ttttgaggat ttcgagaagg cctttgcgga
    11881 attggaacag atctacattc agatgagcca tattggcaac tatgctttta tgcctcagac
    11941 gacggactat agcaatgacg aatttgccaa tattgcccaa gctgggatgg aatttgaaac
    12001 agatgccagc gtagccttga ccttctttga cgatgccttg gtggtagcag acgaggaagt
    12061 cttggaccgt ttgggtgaat tgcctcactt gacggcagct attcgtcagg ccaaaatcaa
    12121 aaaagcccac tatctagggg ctgatgtgga gaaggccttg accaatctcg gtgaagtttt
    12181 ctacagtccg caggacattt atactaagat gcgagctggg gattttgaaa tggctgactt
    12241 tgaagcccat ggcaagacct ataaaaacag ctttgtcacc tatgagaatt tctaccaaaa
    12301 ccatgaggat gctgaggttc gtgagaaatc cttccgttcc ttctcagagg gacttcgtaa
    12361 gcaccaaaat acggctgcag cagcctatct ggctcaggtc aagtctgaaa aactcttggc
    12421 tgatatgaag ggatacgact ctgtatttga ttatcttcta gctgaacaag aagtggaccg
    12481 tgtcatgttt gaccgccaga ttgacctcat catgaaggac tttgcaccag tcgctcagag
    12541 atacctcaag catgttgcca aggtaaatgg tcttgaaaag atgacctttg cagactggaa
    12601 attggacttg gacagcgccc tgaatcctga agtgactatt gacgatgcct atgatttggt
    12661 catgaagtcg gtagaacctt tggggcaaga atattgtcag gaagttgctc gttaccaaga
    12721 agagcgctgg gtggactttg ctgctaacag tggcaaggat tccggtggtt atgcggcgga
    12781 cccatatcgc gtacaccctt atgtactcat gagctggaca ggccgtttga gcgatgtcta
    12841 taccttgatt catgaaatcg ggcattctgg tcaattcatc ttttcagaca atcaccaaag
    12901 ctatttcaac gcccacatgt cgacctatta tgttgaagca ccgtcaacct tcaatgaatt
    12961 gctactcagt gattacttag agaaccagtc taatgaccca cgtcaaaaac gcttcgctct
    13021 ggctcatcgc ttgacagaca cctacttcca taatttcatc acccacctct tggaagccgc
    13081 cttccagcgt aaggtgtata cattgattga agaaggggag acctttggag caagcaagct
    13141 caacagcatt atgaaggaag ttttgacaga tttttggggc gatgccattg aaatcgacga
    13201 cgatgcagct ttgacttgga tgcgccaagc tcactactat atgggcttgt atagttacac
    13261 ttactctgct ggtcttgtca tctcgacagc tggttacctt catcttaaaa actcagaaac
    13321 tggagctgaa gactggctca atctcctcaa atcaggtggt agcaagacac cacttgagtc
    13381 agccatgatt atcggtgctg atatttcaac agacaaacca ctccgtgata ccatccaatt
    13441 cttgtctgac acagttgacc agattatcgc ctacagtgct gagttggggg agtaagggga
    13501 atagaaaaat tttctagaat gatgtttatg ggcttgttaa gcgtcattct atttgtctta
    13561 gttgtagcta ttttcaaaaa tgttgttgaa ggtcgtgatg ttaaatttga acttactgta
    13621 gaactggtat tgatagttat ttacgtcatt ttatatcaga ttgtatttaa ttgagaattc
    13681 aatattgagt catttaagac tgcctttttg aacagttgct agaaatgtag aggtattgac
    13741 catgtatcaa gatttactaa gaaaaatagc agaagaaaaa ccaaattata accaggaaga
    13801 aatccagtgg ttgtttgatc atttgggaaa tccttctcca gaaattcgtg atgacctttc
    13861 aaatcaaggc ttgcactatc tttcaaaaga aaaggataca acagatttct caagtcagta
    13921 tggttgggtt catgcttttg cacatggagc cgatttactg acagaggtgg tttgtcatcc
    13981 agactttcct ataaacagaa ttcatgaagt atttgacata cttggccaac tatttaaaag
    14041 aatgtcgatt cgctttacag atgatgagga ttggcgttta gcaagagtga tctatgaacc
    14101 tattttacaa gggaagttgg agcaagaaca agtagcttct tggataaaaa ctgttgattt
    14161 tccgatagaa gaaagggagg atttttacaa attttccaac tttagatcct gtctggtgga
    14221 agtctatgtc caacttgacc agagaaatag tttacaagat gacctgaaag aagctatcca
    14281 gtcttttcaa tactagagat tagctaatag ttctatgatt ttcaaaaaat ttccaatcaa
    14341 ttttcttgaa accctttcct acttttgata gactagtatc tagtttttga aaaaaggaga
    14401 aagaaaatga aaaaatttgt tgctgagtta atcggtacgt tcatgcttgt gttcgttggg
    14461 acaggagctg ttgtttttgg aaatggtctt gatggccttg gtcaccttgg aatcgccttt
    14521 gcctttggtt tggcaatcgt ggtggcagcc tactcaatcg gaactgtttc aggtgctcac
    14581 ttgaacccgg ctgtttcgat tgctatgttt gtaaacaaac gtttgtcatc ttcagaactt
    14641 gtaaactaca tccttggtca ggttgttgga gctttcatcg cttctggcgc agtcttcttc
    14701 ctcttgggta attcaggtat gtcaactgct agtcttggtg aaaatgcctt ggcaaacggt
    14761 gtcactgtct ttggtggttt cttgtttgaa gtcatcgcaa ctttcttgtt tgtcttggtt
    14821 atcatgactg tgacttcaga aagcaagggc aatggcgcga ttgctggttt ggtaatcggt
    14881 ttgtcattga tggcgatgat tcttgtcgga ttgaagatta ctggactttc agtaaaccca
    14941 gctcgtagct tggcaccagc tgtcttggta ggcggcgcag cccttcaaca agtttggatt
    15001 ttcatccttg caccaatcgc tggtggagtt cttgcagccc ttgttgcaaa aaatttcctt
    15061 ggaacagaag aataattgaa actcaaaaag ccttgctcct catcttgagg aacagggctt
    15121 tttcgtatga tactcttcga aaatctcttc aaaccacgtc agcttcacct tgccgtaggt
    15181 atggttactg acttcgtcag ttctatctgc aacctcaaaa cagtgtttta agctgacttc
    15241 gtcagttcta tccacaacct caaaacagtg ttttgatctg acttcgtcag ttctatccac
    15301 aacctcaaaa cagtgtttta agctgacttc gtcagttcta tctgcaacct caaaacagtg
    15361 ttttaagctg acttcgtcag tttcatctac aacttcaaaa cactgctttg agcaacctgc
    15421 ggctaacttc ctagtttgct ctttgatttt cattgagtat gagtttagcg gttgtcaatt
    15481 ttctctggat aaaggtcgtg ttggaagagg cgttgttctg ccaagccctc atacttagtt
    15541 ccttgcttac cgtagttgta gtaggggtcg attgaaatgc caccgcgcgg agtgaatttt
    15601 ccccagactt ctaaatagcg agggtctagc aagttgacca agtctttccc gatggtgttg
    15661 atacagtttt cgtggaaatc tccgtgattt cggtagctaa atagatatag tttgagggat
    15721 tttgactcga cacagagctt gtcaggaatg taggaaatat gaatcgtcgc aaagtctggt
    15781 tgagcagtga tttgtcccag cagagacata tcgaggatat ggtgacgaat gccctgttcc
    15841 ttagcgattt ctctagcaat ttgaatttcg aggtgatgac gttggccgta ggcaaaggtg
    15901 acagcttcga ctgtttcata gtgttgcatg gtccagaaaa ggcaggttgt tgaatcttga
    15961 ccgccactaa agacgaccaa ggctgattga cgtttcatag tactccttcc aaaatgggaa
    16021 atgttcagag cacgcaaaaa gctcccatta gggagctaaa aaataccaaa tcgaggtttt
    16081 tttagcgatg gcatatccca aacatcgtaa tattctactt atatagtaaa atgaaataag
    16141 aacaggacaa atcgatcagg acagtcaaat cgatttctaa caatgtttta gaagtagagg
    16201 tgtactattc tagtttcaat atactatagt ctagcatatt ttttgaaaaa tggcaaaggg
    16261 caagaaaaaa gagaccaaag aaagtacttg gtctctcgtt tgattagctc aattcagcaa
    16321 tgatggcctt gatttgttct gctgtgtgaa cacctgcaac ctgtttgaca acttggccgt
    16381 cttttttgaa gagaagagtt ggaatagaca tgattccaaa agcacgagct gtgtttggat
    16441 tttcatcaac gtccatttta acgattttca agacatcttc tgaaagttct tcagacaatt
    16501 tgtccaagat tggaccttgc atacgacatg gaccacacca agttgcccag aagtctacta
    16561 agaccaaacc gtcttttgtt tcttgttcga atgttgcatc tgtaattgct tttgccattg
    16621 tatttctcct ttttttagtt atattggctt aaatcttgtt tcatgagata gaagaagata
    16681 tctccataag tcccatggta gtccaaatta tgacccttgt aagttaattt ttggacaggg
    16741 tagtagtctg cgacgccgat aaggcaagct tgttgcgaac gttcaaagtc ttcataagac
    16801 tcgaaagtca cagttctttc gttcttgctg gcatctatat aggtaatttc aatcatattt
    16861 aaaactcctt tgtttaatgc taactttatt ttactcctta taaaagagaa tgtcaagaaa
    16921 aatgattgcg cacgcaactt tttttaaaat catcttaaat caagaaatcc aaacctgctt
    16981 ccaagctttc ttcgacagtc ttttgtagcg aggccagtgt cttttgccca tcatttgtca
    17041 ggcagataaa actagagcgt ctatcttgat ggcaacacat gcgactgagt agaccgcaat
    17101 ttttagcttc caagcgagcc accatcctag aaactgcgct cgggctcaga tgaagcttat
    17161 ctggcaggtc aatctggcgt agagattttt cttcagccaa gtccagatag tagagcaggt
    17221 agaactcttt caaggtcaga ctttgctcgc tctgttgggc aatggtctct tccaagagac
    17281 tttcaatttc tttctgacgc cgattgaagt caaaccattt ttccaaatag gtcatagtgt
    17341 ctcctttctt tttagagtca taaaatagaa gaaagtccat taacgggcag tctctgcgtc
    17401 acaagatgat tgcgcatgca ataattatac tacttttcaa gaatgctggc aagctctgtt
    17461 ttttagtggt tttatttttt actgtccata tttttggtaa aaatcaactt ttacttggat
    17521 gaaggttttg gcttcacgta ggagttgaag aagggtggcg cgggtttcaa actcttctct
    17581 tgtcttgggc agactgcggt tccggaagac ttccagataa ctttcaattt catctagcaa
    17641 atcagaagca ggattggtct ggctcagttg accagcaatt tttgaaaaga gctgcgctaa
    17701 aatcaggctt tcactggcgg caaggtgaca agtgttaatc tgttgggcca tgtttctcag
    17761 gatacgactt tgtcgttgtc tcatctcaaa gtagtggata tggtagtcgg tctggtgaaa
    17821 gaggtggtca gagtgatcca aatagaccag tctgagggct tctttcaaaa gcgtgtctaa
    17881 ttctgctacc agctgtgctc ggttgcgtcc gtctcctctg gataaatagt atttgaagcg
    17941 ctggaggata tcttttaact tttcttccac cagcgtgtgg tagtgctgga tttcctcttc
    18001 tcgtgaaggc atatagagat taacaagcaa ggcaaatcct gtaccaatag caaagagaag
    18061 gaattcattg actagaaggt ctggagaggt tgactcttga accaagagat ggctaaccaa
    18121 aacagtgctt ggtgtgatgc caatttccca gcccatcttg taggctaaag gaacgtagaa
    18181 ggccagatag aggccgagac tccagatatg aaatccgctc aagtgaaaag ctagaacacc
    18241 gatagccaga gctagaagca tagaaaaaag acgattgcga gccagtttta aagtacttct
    18301 acgcgtatca gataggctca agagagcgat aattccagcc gaaactgctg acgaaagatt
    18361 gagaaaataa gcaagcaggc aggcaagaca ggtagctaag atgagcttgg tcgtacgttg
    18421 gctaatagac ataagaattt cctaataagt tagaataaaa gcgtaaaaga caagacatga
    18481 gcaggcttgc cttgatgagt tattttttac gggttgctgc gtattcggca acggcggtaa
    18541 agaggacatc tgtagaagag ttaagggctg tttcacatga gtcttggatg acaccaatca
    18601 caaaaccaac cccaacaatt tgtatggcaa tatcgttaga aataccgaaa aggctacaag
    18661 caactgggat aagaaggagg gaacctccgg caatacctga agcatcacag gctgagatag
    18721 ctgctaccac actgaggaca aaggctgtgg caaagtcaac aggaattcca agagtgttaa
    18781 ctgtaacaag ggtcaaaacg ttaatggtaa tcgctactcc agccatattg atagtagaac
    18841 cgagtgggat agaaacagaa taggtatctg ggttgagtcc aaggtcatgg cagagtttca
    18901 tgttgacagg aatgttagtc gcagaactac gagtgaaaaa ggctgtcaca ccgctgacac
    18961 ggaggcagtt ccaaactaga gggtaaggat tgcgtctcat aaagaagaag gcaatcaaag
    19021 ggttgaccac aggggcaaca aaaagcatag tcgttactaa tagaaccaat aaaataccgt
    19081 agttggcaag gcttccgact cccttgtcag aaatggtttt aaaaacaaga ccaaggattc
    19141 caaatggagc cagattgatg atccattcga caattttaga agtcacgtca gcgatagttt
    19201 ttagcaattc tttactattt ttactggctt ctctcatagc gattccaaaa atgactgccc
    19261 aagataagat tctaatatag ttagcagtaa gcagggcgtt gactgggttg tcaaccagtt
    19321 tgagcaagag gttgctgagg acctgcccaa tcccatctgg tggtgcaatt tcagtattgg
    19381 cactatttag ggtaatttca atagggacga tgaaacttgc tagtacagct acaagagcag
    19441 cggcgaaagt ccctatcata ggatatacaa gaaaacaaca gttttcatat tgctatcttg
    19501 tcccttttga tgttggaaag ggcattggca acgagggcaa agactaggat aggagcaaca
    19561 gcttttagac ctccaacgaa taaatccccg agtagcccaa tccctgagag attaggaagg
    19621 gtcagtccta ggattcccca caaagcatac caatcaagat acgcttgaca aggcttgcct
    19681 tattccaagc atgaatgatt cttttcataa taatctcctt tttgtgtagt gattatgatt
    19741 atagtataaa tgatagacaa aatcaagaat tttctgtcta ttttttgaat atttatggag
    19801 aatgagactg atgaaaatat ggtataatga aataaaggag ttttatatgc aaaaatttat
    19861 tcaggcttat attgaaaagc tagatgtgac aaccattatc gagaatattc taaccaaggt
    19921 catttctctt ttactgcttt tgattgtatt ttatattgct aaaaaaatgc ttcataccat
    19981 ggtgcagaga attgtcaaac cttctctaaa aatgtctcgt catgatgtcg gacgccaaaa
    20041 aaccatctca cgtttactag aaaatgtgtt taattatacg ctatatttct ttttactcta
    20101 ctgcattttg tcgattttag gtttgccagt ttctagtttg ctggctggag ctggtattgc
    20161 tggggtagcg attggtatgg gagcccaagg ctttctgtct gatgtcatca atggcttttt
    20221 catcctcttt gaacgtcaac tggatgtggg agatgaggtc gttctgacaa atggaccgat
    20281 tactgtatcg ggtaaggttg tcagtgtggg aattcgtacg acacagcttc gtagcgagga
    20341 gcaagccctt cactttgtcc ctaaccgaaa tatcacagtt gttagcaatt tctcacgcac
    20401 agactagacc tgttatttta agtaatttgt ggtacaatag agggagttta ataaggagaa
    20461 aagatggttt tagaaaagca gttgggcaat ggttgtacct ggatagacct agacctagga
    20521 aagttgaata aactagaaga cctttctgaa atttacggtt tggacaagga aaccattgaa
    20581 tacgcactgg atagaaacga gcgcgcccac atggattacc accgtgaaag tgggacggtt
    20641 acctttatct ataatgtcct agacgtaaaa aaggacaagg cctactatga gacttttccc
    20701 atgaccttta ttgtcgagca tcgtcgcctg attaccatta gtaataccaa gaacgcctat
    20761 gtcattgaac agatgactcg ttatctggag agccatgaca cgctttcgat ttataagttt
    20821 ctctttgcca gtctggaaat tatcagcaat gcctactatc ctgtcattga gcagatggac
    20881 aagagtaggg atgaggtcaa tgacctcttg cgccagcgaa ctaccaagaa aaacctcttt
    20941 gccctgtctg atttggagac tggtatggtt tatctgacgg cagctgccaa acaaaatcgg
    21001 attttgttag agcatattca aggtcatgcc ttgtatcgta gttttgatga gattgagaga
    21061 gaacagtttg atgatgccat gattgaggct catcagctgg tatccatgac agacctaatc
    21121 tctcagattt tacagcagct ttcagcctct tacaacaata ttctaaacaa taatctgaat
    21181 gacaatttga caaccttgac tatcatttca gtcttgctag ctgttttggc agtcgtgaca
    21241 ggctttttcg gaatgaatgt tcccttacct ttaacagatg agccccatgc ttggctctat
    21301 atcagtttgg ctagtgcagg tttgtggatt gttttatcct tgttactaag gaaaattgcg
    21361 aaaaaaagtt aagaaaagga gccagaatgg cgattgaaaa ttatatacca gattttgctg
    21421 tggaagcagt ctatgatctg acagtcccaa gcctgcaggc gcagggaatc aaggctgttt
    21481 tggtcgattt ggataatacc ctcattgctt ggaacaaccc tgatggaacg ccagagatga
    21541 agcaatggct acatgacctt cgggacgcgg gtattggcat tatcgtagtg tcaaataaca
    21601 ccaaaaaacg cgttcaacga gcagttgaga aatttgggat tgattacgtt tactgggcct
    21661 tgaagccctt cacatttggt attgaccgtg ctatgaagga attccactat gacaaaaagg
    21721 aagtggtcat ggttggcgac cagctcatga cagatatacg agcagcccac cgtgcaggga
    21781 ttcggtcaat tttagtcaaa cccttggtcc aacatgactc aatcaaaacg cagattaacc
    21841 gaactcgtga gcgtcgtgtt atgagaaaaa tcactgaaaa gtacggaccg attacatata
    21901 aaaaaggaat ttaactatgg aagaaattct ctgtattggt tgtggagcaa ccattcagac
    21961 gacagataaa gctggtcttg gttttacccc ccagtcggca cttgaaaaag gtttggagac
    22021 tggcgaagtc tattgccaac gctgtttccg tctccgccac tacaatgaaa tcacagatgt
    22081 ccagttgacg aacgatgatt tcctcaagct cttgcacgag gtgggagaca gtgatgcctt
    22141 agtggtcaat gtcattgata tctttgattt taatggatct gtcatcccag gtttaccacg
    22201 tttcgtctcg ggcaatgatg tcctcttggt aggaaataaa aaagatatcc ttcctaagtc
    22261 agttaagtct ggtaagatta gccagtggct catgaaacgt gcccatgaag aaggtcttcg
    22321 tccagtcgat gtggtcctaa cttcagcaca aaataaacat gccattaagg aagtcattga
    22381 caagattgaa cactaccgta agggccgcga tgtctatgtg gtcggtgtga ccaacgttgg
    22441 aaaatcaact ctaatcaatg ctattatcca agaaatcacg ggtgatcaga atgtcatcac
    22501 tacttcacgc ttcccaggta caaccttgga caaaatagag attccgcttg acgacggatc
    22561 ttatatttac gatacgccgg gaattatcca ccgccaccag atggctcact acttgacggc
    22621 caaaaacctc aagtatgtca gtcctaaaaa ggaaatcaag cctaagacct atcagcttaa
    22681 tcctgagcaa accctatttt taggtggttt gggacgcttt gactttatag caggagaaaa
    22741 gcaaggattt actgctttct ttgataatga actcaaactc catcgtagca agcttgaagg
    22801 agctagtgct ttctacgata agcacctggg aactcttctg acaccaccaa atagcaagga
    22861 aaaagaagat ttcccaaggc tagtccagca tgtctttacc attaaagata agacagacct
    22921 agtcatctca ggcctaggct ggattcgtgt aacaggcata gcaaaagtcg ccgtctgggc
    22981 accagaaggc gtcgccgtcg tcacacgaaa agcaattatt taaaacaaaa tcgctgagcg
    23041 aacaaagtga gctcatatag cgatagttat tgccgtggtg tgattgccaa tctgtagatt
    23101 ggcaaggggc acaattgaga ccaaggctca attgtgaggt caggaaatcc atttttcaaa
    23161 gatgaaatct ttggaaaatt agttccgacc gtccctcacc acctaaaata actatcaaaa
    23221 aacaaggaag aaaattatgt cattaacatc aaaacaacgt gccttcctca acagccaggc
    23281 acacaccctc aaacctatca tccaaatcgg gaaaaatgga ctcaacgacc aaatcaaaac
    23341 cagcgtccgt caagctcttg atgcgcgtga attaatcaag gttactctct tacaaaacac
    23401 agatgaaaac atccacgaag tagctgaaat tttggaagaa gaaatcggtg tggatacagt
    23461 ccaaaaaata ggacgcatct tgattttgtt taagcaatct agcaagaaag aaaatcgcaa
    23521 gatttctaag aaagtcaaag aaatctaaga ccgaaactcc aaataactgt ttttatagag
    23581 aaataaaggg gactagccta tgacaatcga actattgact ccctttacca aggtagagtt
    23641 ggagccagaa atcaaggaga aaaaacgcaa acaagttggg attttagggg ggaattttaa
    23701 ccctgttcac aatgcccatc tcattgttgc ggatcaagta cggcaacagt tgggactgga
    23761 tcaagttctt ctcatgcctg aataccaacc tcctcacgtt gataaaaagg aaaccatccc
    23821 tgaacaccat cgtctcaaga tgcttgagtt ggcaattgag ggaattgacg gcctagtcat
    23881 tgaaaccatt gagttggagc gcaagggtat ttcctacacc tacgacacca tgaagatttt
    23941 gacagagaag aatccagata cggattatta ctttatcatc ggtgccgaca tggttgacta
    24001 tctgcctaag tggtaccgaa ttgatgaact ggttgacatg gttcagtttg tgggggttca
    24061 gcgtccacgc tacaaggtag ggacttccta tccagttatc tgggtggacg taccgctcat
    24121 ggatatctcg tccagcatgg tgcgggactt ccttgcccaa ggtcggaaac ccaactttct
    24181 cctacctcag ccagtgctag actacatcga gaaggagggg ctctactgat gtcctatcaa
    24241 gactatatca actgctcccg tgaggccttg ttggaaaaaa tggcagagtt tctacctgag
    24301 aaacgtctaa cccattgtct aggtgtggag cgtgccgcta tggaattagc tcagcgcttt
    24361 ggtgtagatg ttgagaaagc aagtctagca ggtcttcttc atgactatgc taaaaagttg
    24421 tcaaatcagg aatttctagc cttgattgac cgttaccagc tagaccctga cctcaaaaac
    24481 tggggcaata atgtctggca tggtatggtc ggaatttaca aaattcagga agatttggat
    24541 ttgcatgatt cagaaatcct gcgagccatt gaagtccata cagtcggtgc tggtcaaatg
    24601 acagacctag ataaggtcat ctacgtcgca gactatatcg agcacaatcg agcctttcct
    24661 ggagtagatg tggcgcgtga gattgcaaat ctatcgctca ataaggcggt ggcctacgaa
    24721 acagctcgta ccgtggagta tctagctcat cagggattcc ccatctatcc ccaaaccctt
    24781 gaaacctata acgcctttgt gcactatttg aaagagaact aaatgaagac ggcttttata
    24841 atcattgatg ttcagaatgt tttagtagaa actgggttcc agacaaaaag tctattggga
    24901 aaaatttctt atttacaaaa ccaggctaga agcaagaata ttgaaattat ctatgttcaa
    24961 catattgaga actctgaagc tcaaacatca gaagattggc agttatctgc gcttttaaat
    25021 cgaaaacctg ctgaaaaggt ctttcagaag aagtataaca gtattttcaa agaaactggc
    25081 ttaaaagaat acttggataa gcaggggatt gaaaaattag ttttatgtgg tatgcagaca
    25141 gaatattgtg tggatacctc tgtcaaggtt gcctttgaat atggctatca gcttattgtt
    25201 ccagaaggtg ctgtcacaac ctttgatggg gatgacattc cagcagaaac gattaatgaa
    25261 ttttatgagg acatttggga ggagcgcttt gcagatgttc tagattacaa acatattttc
    25321 taaaaagagg attaaatgaa cgaaaaagaa ttactagaac tagtcgtgaa agcggctgat
    25381 gagaaacgtg cggaggatat cctcgcactt gatgtacaag atttgactag tgtgacggac
    25441 tactttgtca tcactagctc aatgaatagc cgtcagttgg acgctatcgc agctaatatc
    25501 cgtgaaaaag tagctcaagc aggctttaaa ggtagccatg tcgaaggcga tgcagctgga
    25561 ggctgggtct tactggacct tggtgctgtt gtcgtgcata tcttttcaga agaaatgcgt
    25621 gcccattata atctagagaa gctatggcat gaggcgaatt cagtagatat ttcagaagct
    25681 cttgcttaga agatgaactc agttgtagtc aactgggttt ctttgcttat tttagaaaaa
    25741 ttgatagaaa ggtaaagggc tagtggtgtt taccatggag ctcttagtat catgattatg
    25801 gcgacttatg aaacctttgc ggcggtctat gatgcggtca tggacgatag tttatacgac
    25861 aaatggacga atttttctct gcgtcatttg cctaagacca aggagagaaa gaaactcttg
    25921 gaattggctt gtgggacagg aattcaatca gtgcgctttt ctcaggctgg ttttgatgtg
    25981 actggacttg acttgagtgc ggatatgttg aagattgcgg agaagagagc aacttcagcc
    26041 aagcaaaaga ttgcttttat agaaggtaat atgctgaatt tgtccaaggc agggaaatac
    26101 gattttgtca cgtgttattc ggactctatc tgctatatgc aggatgaggt ggaagtaggg
    26161 gacgtcttta aggacgtgta caatgcgctg aatgaagagg gagttttcat ctttgacgtg
    26221 cactcgacct accagacaga tgaagtattc cctggctatt cctaccatga aaatgcggaa
    26281 gattttgcca tgctttggga tacatatgag gacgaagttc ctcactccat cgtgcatgag
    26341 ctgactttct ttatcaagga ggctgacggt tcctttagtc gccacgatga agtacatgag
    26401 gagaggacct atgagatctt gacctatgat atcttgctgg aacaggctgg atttaagtcc
    26461 ttcaaactct atgcagactt tgaggataag gagccaacag aaactagcac ccgttggttt
    26521 tttgtggcgc agaagtagga gattagcatg accatcacag gtattatcgc ggagttcaat
    26581 ccttttcata atggccataa atacctgctg gatcaggcag agggactgaa aattgttgcc
    26641 atgtcaggga atttcatgca gcgtggagag cctgctattg tggataagtg gacaagggcc
    26701 cagatggcgc tggaaaatgg cgcagacttg gtagtggaat tgcccttttt agtcagtgtt
    26761 caggcggcag attttttcgg tcaaggatct gtggatatct tggatcgctt gggcatagat
    26821 agcctcgctt ttgggacaga agaagttcta gattatcaaa aaatcgctga cttatacaca
    26881 gagaaaggtg ctgagatgga gaaatttgtg aaaaatctgc ctgattccct ttcctatccc
    26941 cagaaaaccc aagctatgtg gaaggaattt gctggtcttg atttttcagg taataccccc
    27001 aatcatgttc ttgctctggc ctatgccaag gcggttgcag gacggaatat caagctgcat
    27061 ccgattcagc gtcagggggc aggttaccat tctgtgaata aagatgtgga ctttgcctcg
    27121 gcgacagccc tccgtcaaca tcagaaggac caagatttct tagaacgttt tatgccttct
    27181 gttgccctat ttgagcaggc cagtaaggtg atctgggaag actattttcc cttgctccgc
    27241 tatcaaatct tgtcaaatcc cgacctaacc accatctatc aggtcaatca agaaatggct
    27301 gtgcgcatta aagaagctat taaaacagcc cagtctgtgg aagaattggt cgagttagtg
    27361 actaccaaac gttacaccaa ggcgcgtgtc agacgcctat tgacctatat tttgatgcag
    27421 gctagagaaa gtgacttgcc agaagccatt catgtccttg gatttactga aaaaggtagg
    27481 caacatctca agtctttgaa aggtcaggtc agtctagtca gccgaattgg caaagaacct
    27541 tgggatgcta tgactcaaaa ggttgaccag atttaccaac taggaaagcc aagtatagca
    27601 gagcaaaatt ttggcagagt gccaattaga atagaaacaa actaagtcta ctgaaaggta
    27661 ggctttttga gattttttac gaataatact cttcgaaaat caaattcaaa tcgcgtcaac
    27721 gtcgccttgc cgtactcaag tacagcctgc ggctagtttc ctagtttgct ctttgatttt
    27781 cattgagtat aaatagatag aaaagaaaat cttgtacaag ttcctgacaa tttctttctt
    27841 ttcgtgtata atagtggaaa agagctaaaa cgaggtatgg ttatggaagc agtcctttac
    27901 tcaacattcc gaaatcattt aaaggactac atgaagaagg taaatgatga atttgagcct
    27961 ttgacggtgg tcaataaaaa tccagatgag gacattgtag ttctttcaaa gagcgaatgg
    28021 gacagtattc aggaaaccct gagaatcgcc caaaataagg agttatcaga caaggtttta
    28081 cgaggaatgg ctcaagtccg tgctggaagc actcaggtcc atgtgattga ggagtgatga
    28141 atgctactca agtttacaga agatgcctgg gcagattatt gctactggca aaaccaagat
    28201 aaaaaaacac taaaaagaat caataaatta atcaaggata ttcaacgcga tccatttaca
    28261 ggaataggaa aaccagagcc actcaaatat gattaccaag gagcctggtc acggcgtatt
    28321 gatgcagaaa atcgcttgat ttatatgatg gatggagata gcgtggcttt cttgtccttt
    28381 aaagatcatt actaagtcta ctgtaaagta ggctttttct caagtctcta ctaaactact
    28441 atttctaaac aacaagttta tttttgcaaa tgtgttccaa attccccaaa acatattcta
    28501 ttttaggttt gtgaaaatca cttttttatg gtagaatgta aaagaatgta tgtcattcgg
    28561 aataacaata aaatgaggta aaaacatgga aatcatgtcg cttgcgattg ctgtttttgc
    28621 cgtcatcatt ggtttagtca ttggatatgt cagcatctca gctaagatga aatcatctca
    28681 ggaagctgca gagttgatgc ttttaaatgc tgaacaagaa gcaactaatt tacgtggaca
    28741 agctgagcgt gaagcggatt tacttgttaa tgaagccaaa cgtgaaagca agtctcttaa
    28801 aaaagaagca ctattggagg ccaaagaaga agccagaaaa taccgtgaag aagtggacgc
    28861 tgaattcaaa tcagaacgtc aagaactcaa acaaatcgaa agtcgtttga cagagagagc
    28921 tactagcctt gaccgtaagg acgacaattt gacgagtaaa gaacaaacac ttgaacaaaa
    28981 agaacaaagt atttctgata gagcgaaaaa ccttgatgcg cgtgaagagc aattagagga
    29041 agtcgaaaga caaaaagaag cagaactaga gcgtattggt gctctgtctc aggcagaagc
    29101 acgagatatt atcttggctc agacagagga aaacttgacc agggagattg ccagtcgcat
    29161 tcgcgaagct gagcaagagg tcaaggaacg ttctgacaaa atggccaagg acatcttggt
    29221 tcaagctatg caacgtatcg ctggtgaata tgtagcggag tcaacaaact caacggttca
    29281 tctgccagac gatactatga agggacgcat tattggtcgt gaaggtcgta acattcgtac
    29341 ctttgaaagt ttgacagggg tcgatgtgat tatcgacgat acaccagaag tggtgacctt
    29401 gtcaggattt gatccgattc gtcgtgagat tgcccgtatg actatggaaa tgttgctcaa
    29461 agatggtcgt atacatccag ctcgtatcga agagttggtt gagaaaaacc gtcaagagat
    29521 tgacaataag attcgtgaat acggtgaggc tgctgcctat gaaattggtg cgccaaacct
    29581 tcatccagac ttgatgaaga ttatgggacg tttgcagttc cgtacttcat atggacaaaa
    29641 tgttttgcgc cattcgattg aggttgctaa gttggctggt atcatggcga gcgaacttgg
    29701 tgaaaatgcg gctcttgccc gtcgtgctgg attccttcac gatatcggga aagccattga
    29761 ccatgaggtt gaaggtagcc acgttgaaat cggtatggaa ttggcccgta agtacaagga
    29821 acccccagtt gtggtgaata cgattgctag tcaccacgga gatgttgaag ctgagagcgt
    29881 gatagcagtt atcgtcgctg cagcagatgc cttgagcgca gcccgtccag gtgctcgtag
    29941 tgagtctctt gaaagctaca tcaagcgtct ccatgatttg gaagaaattg ctaacggctt
    30001 tgaaggagtg caaactagct ttgcccttca agcaggacgt gaaattcgta tcatggtcaa
    30061 tccaggaaaa atcaaggacg acaaagtcac aatcttggct cacaaagttc gtaagaaaat
    30121 tgaaaacaat ctcgattatc caggaaatat caaggtaacc gtgattcgcg agcttcgtgc
    30181 agtagattat gctaaataaa taagaaagag cagttgaaaa attactgctt ttttgttaca
    30241 ctagatagaa agactgtaaa ggataatctg gctttttgcc attattctag agaaatgtta
    30301 tttcacagat tgactaaaag gagacacaat ggcagaccga ggcttactaa tcgttttttc
    30361 tggtccttca ggggttggaa aaggaacggt tagaagagag atttttgaga gttctgaaaa
    30421 ccaatttcaa tattctgtat cgatgacgac acgcgcacaa cgtcctggag aagtggacgg
    30481 tgttgactat ttcttccgta ctcgtgaaga atttgaagag ctgattcgtc aaggacagat
    30541 gttggaatac gcagaatatg tcggcaacta ctatggaact cctctgacct atgtcaatga
    30601 aaccttggac aagggaatcg atgttttcct tgaaattgaa gttcagggtg ctcttcaggt
    30661 caagaaaaag gttccagatg ctgtctttat cttcctgaca ccaccagatt tggatgaatt
    30721 gcaagatcgc ttggtaggtc gtggaacaga tagtgcagaa gtgattgccc aacgaatcga
    30781 aaaggccaag gaagaaattg ccctcatgcg tgagtatgat tatgcgattg tcaacgatca
    30841 ggtgccccta gctgctgaac gtgtcaaatg tgtgattgaa gcagaacact tctgtgtgga
    30901 tcgtgtcatt ggtcactatc aggagatgtt accaaaatct ccaactaccc gataaaattt
    30961 agaaaatagg tacaagcaaa tgatgttaaa accctctatt gataccttgc tcgacaaggt
    31021 tccttcaaaa tattcactcg taatcttgga agcaaaacgt gcccacgaat tggaagcagg
    31081 tgccccagca actcaaggtt ttaagtctga aaaatcaact cttcgcgctt tagaagaaat
    31141 cgaatcagga aacgttacaa ttcacccaga tccagaagga aaacgtgaag cagtgcgtcg
    31201 ccgtatcgaa gaagaaaaac gccgcaaaga agaagaagaa aagaaaatca aagagcaaat
    31261 tgctaaagaa aaagaagatg gtgaaaaaat ttaaggttgg ggggactcaa tcttattttt
    31321 tctattgcaa gaatgtactg acaaggagga ggtgagacga tggccctagc taagattatc
    31381 gtagatgtgc ccttgatgca gacggaccag ccctatagtt acaggattcc ggaggaattt
    31441 gagggaatgc tggaagttgg gatgcgggtt catgtgcctt ttggtaaggg gaatcgcctg
    31501 attcaaggga ttgttcttgg tttggagtcc caatcagatg gagaagagat ggagcaagat
    31561 ttaaaagata ttgcagaggt gctggatttt tctcctgttc tcacgccaga acaactctgg
    31621 ctggctgagg agttacgcaa atctgtcttc tcctacaaaa tttctatcct caaggctatg
    31681 cttccaggat ttctgaattc tagctatgac aagattctct atcctctgga aggcttgagt
    31741 caggaagaac gagtgcgcct gtttggttca gaagattcgc tagccttttc ttcactagac
    31801 cttgccaagc aagctgaaat gatgcgtttg actagaaaag gcctgcttgg tctggaatat
    31861 caggcagtcg atcaaaagaa ggtcaagacc cagtcttggt atgaggttga ccatgctcaa
    31921 ttagaaggtg ttgagatttc tacacgtgcc aagaaaaagt tggaactgag agattatctg
    31981 ctgtctcatc cagagagcgc ttccttggct agcttgttag agtcctactc gcgagagcaa
    32041 gtcaacttct ttgtggatca aggtgctgtt accatagtcc aaaaggaagt tcaacgctcg
    32101 gctgcttatt ttgaaggaat tgaagcaagt agacctttgg agttaaatcc agaacaaaga
    32161 caggcgcgtg atgcggttgt gagttctatt ggcagttctc aacctccctt tctacttcaa
    32221 gggattacag gaagtgggaa gaccgaggtt tacttgcaga ttatccaagg tgccctggat
    32281 aagggcaaga cagcgatttt gctggtacct gagatttcct tgactcctca gatgaccgag
    32341 cgttttatag cacgttttgg ggacaaggta gccattcttc actcaggatt atccaatggt
    32401 gaaaagtatg atgaatggcg caaggtggag cgtggcgatg cccaagttgt tgttggtgcc
    32461 agatctgcta tctttgctcc tctgaaaaat ctgggtgtca tgattattga tgaagagcat
    32521 gaagcggctt ataagcagga cagcaatccc cgttaccatg ccagagaggt agctatttta
    32581 cgggctcagt ataatcaagc aactctggta cttggatctg caacgccgag cttagagagc
    32641 cgggcacgtg ctggcaaagg cgtctatcaa cacttacgtc taacccaacg tgccaatcct
    32701 ttggctacaa tccctgaggt tcaagtgatt gactttcgag actatatcgg acaaaatgag
    32761 acgtcaaact ttacgcctcc tttgctagag gctatccaag accgtctggt taaaaaagag
    32821 caggtggttc tcatgctcaa tcgccgtggt tattctagct ttgtcatgtg tcgggagtgt
    32881 gggacggtgg atacttgtcc caactgcgat atttctctga ccttgcatat ggataccaag
    32941 actatgaact gccattattg tggtttctcg aaggatattc ctcaggtctg tcctaactgt
    33001 aagagccgca gtattcgtta ctatgggacg ggaactcaga aggcttatga tgagctagca
    33061 gaactctttc cccaagctcg cattttgagg atggatgtgg atacgactcg aaagaaaggc
    33121 agtcaccaag ccttgcttga ccagtttggg cgaggggaag cggatatttt acttggtact
    33181 cagatgattg caaagggttt ggattttccc aatgttaccc tagtcggagt tctaaatgcg
    33241 gatacggcct tgaatctgcc tgatttccgt tcttctgaga gaaccttcca gctcttgact
    33301 caggtggcag gtcgagcagg tcgggctgaa aaagcaggtc aagtcttgat acagtcctac
    33361 aattcgcagc actatgctat tcgatttgcc aaggatcaag attacgaagg cttttatgcc
    33421 tatgaaatgg gaatcagacg acaactcggc tatccacctt actatttcac gattggtatt
    33481 accctttctc acaagaaaga agaagaggtt ttcaaacgtg cctatgaagt catgaacatt
    33541 ttgcggtcag gcttgtcaga gaccagtccc attctggggc caacgccaaa accgattgcc
    33601 cgtacccaca acctctatca ttaccagatt ttaattaaat accgtttaga agatgagctg
    33661 gggccgaccc tcaaccaggt cttggccttg actcaagaac gggaaaatag tgagctccgt
    33721 ctcagcattg accatgagcc gcagcaattt ttataagaag gagaagatat gacaaaacta
    33781 atctttatgg ggacccccga cttttcagca acagtcttaa aaggactttt gacagatgac
    33841 cgttacgaaa ttctagccgt tgtgacccag ccagaccgtg ctgttggtcg taaaaaagtt
    33901 atccaagaaa ccccagtcaa gcaggctgcc aaggaagcag gactatctat ctaccaacct
    33961 gaaaaattat ctggaagtcc agagatggaa gaacttatga agctaggagc agatggaatt
    34021 gtgactgctg cttttgggca gtttctccca agcaaactcc ttgatagcat ggactttgct
    34081 gtcaacgttc atgcctccct ccttcctaga caccgtggtg gtgcgcctat ccattatgcc
    34141 ttgattcaag gggatgagga agctggtgtg accatcatgg aaatggttaa ggaaatggat
    34201 gcaggagata tgatttctcg tcgcagcatt ccgatcacag atgaggacaa tgttggcacc
    34261 ttgtttgaaa aattggcgct agttggtcgt gatttgcttt tggacactct gcctgcctat
    34321 attgctggtg atatcaaacc tgaaccgcag gatacgagtc aggttacctt ctctccaaat
    34381 ataaagccag aggaagaaaa actggactgg aacaaaacca atcgtcaact ctttaaccaa
    34441 attcgtggaa tgaacccctg gcctgttgcc catactttcc ttaagggcga ccgctttaag
    34501 atttatgaag ccctaccagt agaaggtcag ggaaatccag gtgagattct ctctatcggc
    34561 aagaaagaat tgattgtcgc aacggctgaa ggggctctat ccctcaaaca agtgcagcca
    34621 gctggtaagc ctaagatgga cattgcttcc ttcctcaacg gagttggacg tacattgact
    34681 gtaggagaac gatttggtga ctaaagtaga aacggctaga agtttagctc tagcagtgct
    34741 agaggatgtt tttgtgaacc aagcatactc aaatatcgcc ttaaataagc atctaaaggg
    34801 gagtcaactt ttggcagcag acaagggctt agtgaccgag ctggtctatg gaacggtagc
    34861 ccgtaaactg actctggaat ggtacctatc ccactttatc gaagacagag accagttaga
    34921 cagctggctc tatgtccttc ttctcatgag tgcctaccaa ctccgctatt tggacaagat
    34981 tccagatcat gctgtggtca atgaagcagt ggaattagcc aaactccgta aaaaaggcag
    35041 tgaaaaattg gtcaatgccc tccttcgccg tatcttgcgt gaaggctggc cagatattgc
    35101 tagcatcaag cgaaaaaaca aacgtgactc cattgcctat tctctcccag tttggctagt
    35161 tgccaaactc aaggaagaat acggagagga gagagcaaaa gccatctttg aaagcctgtt
    35221 agtgcgaaac aaagccagta ttcgtgtaac agacctaagt cgaaaagagg aaatccaagc
    35281 cttgttggag gcgaatgatt cccttttagc aaccactggt ctggttaagg agcaggggca
    35341 ttttgcaggg cataatttgt ttgcggatgg agccattacc atccaagacg agtccagtca
    35401 gctggttgct ccgacgcttg atttacaagg tgatgagcga gtgcttgatg cctgtgcggc
    35461 tccaggtggg aaaacagccc atatagcctc ttatcttacg acaggtcagg ttactgctct
    35521 ggacttgtac gaccacaagt tggatttaat tcaagaaaat gcccaacgtc tgggagttga
    35581 agatcgggtt caaactcaaa aattggatgc cagaaaggtg catgagtttt ttgaccagga
    35641 ttcctttgat aagattttgg tggatgctcc ttgttcagga atcggtcttt tgcgccgaaa
    35701 accagatatc aaatacaata aagaaacggc agatttcgcg tccttgcagg aaattcagtt
    35761 agaaatatta ggtagtgttt gtcaaatact aggcaaaggt ggtataataa cttatagcac
    35821 ctgcactatc gtctcagagg agaattttca agtcgttaag gcctttttag agagtcatcc
    35881 cgagttcgag caggtaaaac tagaacatga atgtaaggat atcatgaaag acggctgtat
    35941 cctcattaca cctgaattgt atggaagtga tggattcttc atcagtcaat ttcgcaagat
    36001 atcggattag gaaggaactg acacatggaa atttcattat taacagatgt tggtcagaaa
    36061 cgaacaaata accaagacta tgtcaaccac tatgtcaata gagctggacg taccatgatt
    36121 attttagctg atgggatggg aggtcatcgt gcagggaata tcgctagtga aatggcggtc
    36181 acagacctgg gtgtagcttg ggttgatacc cagatcgata cagtcaatga agtgcgtgaa
    36241 tggttcgccc attacctaga aattgaaaat caaaagattc accagcttgg tcaggatgaa
    36301 gcttacagag gcatgggaac tactttggaa gtccttgcta ttattgataa tcaggctatc
    36361 tatgctcata ttggtgattc gcgtatcggc ttgattcgtg gagaagaata ccatcagttg
    36421 acgagcgatc attccttggt taatgaattg ctcaaggctg gtcaattgac accagaagag
    36481 gcagaagctc atccgcaaaa aaatattatc acccagtcta ttgggcaaaa agatgaaatt
    36541 cagcctgatt ttgggacagt tatccttgag tcaggtgact atctcttgct caatagtgac
    36601 ggcttgacca acatgatttc aggcagtgag attcgtgata ttgtaaccag tgatattcct
    36661 ttagcagata aaacggagac acttgttcgt tttgctaaca atgcaggagg tttagacaac
    36721 attacggttg cccttgtttc tatgaacgag gaggatgcag aatgatccaa atcggcaaga
    36781 tttttgccgg acgctatcgg attgtcaaac agattggtcg aggaggcatg gcggatgtct
    36841 acctagccaa agacttaatc ttagatgggg aagaagtggc agtgaaggtt ctgaggacca
    36901 actaccagac ggacccgata gctgtagctc gttttcagcg tgaagcgaga gctatggcag
    36961 atctagacca tcctcatatc gttcggataa cagatattgg cgaggaagac ggtcaacagt
    37021 atcttgcaat ggagtatgtt gctggactag acctcaaacg ctatatcaag gaacattatc
    37081 ctctttctaa tgaagaagca gtccgtatca tgggacaaat tctcttggct atgcgcttgg
    37141 cccatactcg aggaattgtt cacagggact tgaaacctca aaatatcctt ttgacaccag
    37201 atgggacggc caaggtcaca gactttggga ttgctgtagc ctttgcagag acaagtctga
    37261 cccagactaa ctcgatgttg ggctcagttc attacttgtc accagagcag gcgcgtggtt
    37321 cgaaggcgac tgtgcagagt gatatctatg ccatggggat tattttctat gagatgttga
    37381 caggccatat cccttatgac ggggatagcg cggtgaccat tgccctccag catttccaga
    37441 aacccctgcc gtccgttatt gcagaaaatc catctgtacc tcaggcttta gaaaatgtta
    37501 ttatcaaggc aactgctaaa aagttgacca atcgctaccg ctcggtttca gagatgtatg
    37561 tggacttgtc tagtagcttg tcctacaatc gtagaaatga aagtaagtta atctttgatg
    37621 aaacgagcaa ggcagatacc aagaccttgc cgaaggtttc tcagagtacc ttgacatcta
    37681 ttcctaaggt tcaagcgcag acagaacaca aatcaatcaa aaacccaagc caggctgtga
    37741 cagaggaaac ttaccaacca caagcaccga aaaaacatag atttaagatg cgttacctga
    37801 ttttgttggc cagccttgta ttggtggcag cttctcttat ttggatacta tccagaactc
    37861 ctgcaaccat tgccattcca gatgtggcag gtcagacagt tgcagaggcc aaggcaacgc
    37921 tcaaaaaagc caattttgag attggtgagg agaagacaga ggctagtgaa aaggtggaag
    37981 aagggcggat tatccgtaca gatcctggcg ctggaactgg tcgaaaagaa ggaacgaaaa
    38041 tcaatctggt tgtctcatca ggcaaacaat ccttccaaat tagtaattat gtcggccgga
    38101 aatcttctga tgttatcgcg gaattaaaag agaaaaaagt tccagataat ttgattaaaa
    38161 ttgaggaaga agagtcgaat gagagtgagg ctggaacggt cctgaagcaa agtctaccag
    38221 aaggtacgac ctatgacttg agcaaggcaa ctcaaattgt tttgacagta gctaaaaaag
    38281 ctacgacgat tcaattaggg aactatattg gacggaactc tacagaagta atctcagaac
    38341 tcaagcagaa gaaggttcct gagaatttga ttaagataga ggaagaagag tccagcgaaa
    38401 gcgaaccagg aacgattatg aaacaaagtc caggtgccgg aacgacttat gatgtgagta
    38461 aacctactca aattgtcttg acagtagcta aaaaagttac aagtgttgcc atgccgagtt
    38521 acattggttc cagcttggag tttactaaga acaatttgat tcaaattgtt gggattaagg
    38581 aagctaatat agaagttgta gaagtgacga cagcgcctgc aggtagtgta gaaggcatgg
    38641 ttgttgaaca aagtcctaga gcaggtgaaa aggtagacct aaataagact agagtcaaga
    38701 tttcaatcta caaacctaaa acaacttcag ctactcctta aaagcagatg gataatcaaa
    38761 atgagtaatc aatctttgtc atgatttcat ggcaaagatt ttttttgagt ccagatttgt
    38821 gatagaatag agggagttga taaaaggagg aaagcatgga agaatcaaaa gaattaaatg
    38881 ccgtcattga tgtgattatg ctagcgggga ctattctcct taaaagtggc tcagaaatcc
    38941 atcgtgtaga agataccatg attcgaatcg cgcattcgca ggggattgtg gattgcaatg
    39001 tccttgccat gcctgccgct atctttttct ctattgaaaa taccaatatt tcgcgcatga
    39061 agcgcgtgac ctcctcttct tataacatcg aaaaagtctg cgatgtgaac cagatttctc
    39121 gtcagctagt tggggggcag attgacttag aaacagcctt taagcaattg acggccttgc
    39181 aagctcaacc ccttccctat actaagttgc aggtaactct ggctgcgacc tttagtgctc
    39241 ctttcttttc agttatgttt agcggaaata tctacgacgc acttggggca ggagtggcga
    39301 ccttatttgg ttttgccttt tccctctatg tggaaaaatt tatccgaatt ccctttgtga
    39361 cagcctttgc tggagccttt gtctttggga taattgccca gttttgggct cgctacacag
    39421 gttttccttc aacggcagat ttgattatag ctggtgcggt catgccgttt gtaccaggta
    39481 ttgccttgac caatgcggtc cgtgatatta tgaccaacca cataaactct ggtatgagta
    39541 agatgtttga atccctgctc attaccctcg ctttaggggc aggaacttct gtcgccttgg
    39601 tattgatgaa ctaatatgac actaacaacc tttttattac aagcagtagc aagttttctt
    39661 gccattatca cttttttaat cgtactcaat gtgcaacggt ctatgctctt acctggaggg
    39721 attttgggca tgactgtctg gctaatctat ctcttgctca aggaaccgac caatgtcatt
    39781 gtagctacct tcattgcagc cattattggt tcttgtgtca gccagatttt aagtattctt
    39841 tataagacac ctgctgtggt ctttatcttg gccattttgg caccgctggt tccaggttat
    39901 ctctcctacc gaacaactgc cttttttgtg acaggggact ataataaagc actggcaagt
    39961 gcgaccttgg ttgtcatgtt ggctctggta atctctattg gaatggctag cggaacagtg
    40021 attctcagac tgtatcatta tataaaaaca catcgagtat cgtagacttt acagaaataa
    40081 aagaattttc tgaaaaatga gataaataaa ttaacaacgc tttctatatg tgcgagaata
    40141 ccgcacttat gaagaaatta cggctgattt tggtatccac gaaagcaact taatccgtcg
    40201 gagccaatgg gttgaagtaa ctcttgttca aagtggtgtt acgatttcaa aaactcatct
    40261 tagtgctgag aatacggtga ttgtggatgc aacagaggta aaaataaatc gtcctaaaaa
    40321 aatcaactag ccaattattc tggtaaaata gaaatgtcat tctatggaac ctcaagcgat
    40381 tgtcacaagt caagggagaa ttgtttcttt ggatatcgct gtgaactatt gtcatgatat
    40441 gaagttgttc aaaatgagtc gcagaaatat cggacaagct ggtaaaatct tggttgacag
    40501 tggttatcaa gggctcatga agatatatcc tcaagcacaa actccacgta aatccagcaa
    40561 actcaagccg ctaacagctg aagataaagc ctgtaaccat gcgctatcta aggagagaag
    40621 caaggttgag aacatctttg ccaaagtaaa aacgtttaaa atgatttcaa caacctatcg
    40681 aaatcatcgt aaacgcttcg gattacgaat gaatttgatt gttggtatta tcaatcatga
    40741 actaggattc tagttttgca ggaagtctac tattttcctt attgtctgta agtctactga
    40801 ccttgttgtt tatcccagtc atggtttcta gttcgggctc agagtttcaa agtggatggc
    40861 aagagcatca attgattgct gagaaggtta gtaaaacact tgacaagaca tttgataagg
    40921 atgtcagaga aattccgacc agtcagtttt atcaaaaatt tgtagatgag atgggaagga
    40981 cttactcagg aaatttgatc ctccaggagc tgataactgt gaatggagct tataaagcta
    41041 cttatatcga tgaactctct agcaactaat cattctttaa aatgatgacc aataagaggc
    41101 ttgatttggt gaatcaagtc ttttttctct cttttactgc catttgtgat aaaatagtat
    41161 agaacgattt tttacatgaa tgataaaaca gaggtaaata tgacaatcgg tattgataag
    41221 attggttttg cgaccagtca atatgtcttg aaattacaag acttagcaga agcgagggga
    41281 attgaccctg aaaaattaag taaaggactc ttactcaagg aattgagtat tgcgccccta
    41341 actgaggaca tcgtgacctt ggcggccagt gctagtgact ctattttaac tgagcaagaa
    41401 agacaagaag ttgacatggt cattgtagct accgagtcag gaattgacca gagtaaggct
    41461 gcggccgtct ttgtgcatgg cttgctgggc atccagccct ttgctcgtag tttcgagatt
    41521 aaagaagcct gctatggggc gacagctgcc ctccattatg ccaaattgca tgtggaaaat
    41581 tctccggagt ccaaggtctt ggtcattgcc agtgatattg ccaaatatgg tattgaaact
    41641 ccgggagaac ccactcaggg tgccggaagt gtggctatgt tgattacaca aaatccacgc
    41701 atgatggcct ttaataatga caatgtagct cagacccgtg acatcatgga tttctggcga
    41761 ccaaattact cgacaactcc ttatgtaaat ggtgtctatt ctacccaaca atacttggat
    41821 agtttgaaaa cgacttggct tgaatatcaa aaacgctacc agcttacttt ggatgatttt
    41881 gcggctgttt gtttccactt gccttatcct aaattagcgc taaaaggctt gaaaaaaatc
    41941 atggataaga gcctgcctca agagaaaaaa gacctcttac aaaagcattt tgaccagtct
    42001 attctctaca gtcaaaaggt ggggaatatc tacacaggtt cacttttcct tggacttttg
    42061 tctctcttgg aaaatacaga tagcttgaaa gctggggata aaatcgtcct ttatagttac
    42121 ggaagtggag ctgtggctga gttcttcagt ggtgaattgg ttgaaggata tgaagcttat
    42181 ttggataaag accgcttgaa caagctcaac caacgaactg ccctatccgt tgcagactat
    42241 gaaaaggtct tttttgagga agtaaacttg gatgaaacaa actctgccca gtttgctggc
    42301 tatgaaaatc aagattttgc cttggttgaa attctcgacc accaacgccg ttatagcaag
    42361 gttgaaaaat aatgaagata agttggaatg gattttctaa aaaatcatac caagagcgcc
    42421 tcgagctgct aaaagctcag gcgctcctta gtcctgagag acaagctagt ctggagaagg
    42481 atgaacagat gagtgtgact gtggcagacc agctgagtga gaatgtggtg ggaacttttt
    42541 ctctgcctta ttcgctggtt ccggaggtac ttgtcaacgg tcaggaatac accgttccct
    42601 atgtgacaga agaaccctct gtggttgcgg cggccagcta tgccagcaaa atcatcaagc
    42661 gtgcaggtgg ttttactgca caagtccatc agcgccagat gattgggcag gtagcccttt
    42721 atcaagttgc taatcctaaa ctagcgcaag agaagattgc cagcaagaaa gcggagctct
    42781 tggagcttgc caatcaagcc tatccttcta tcgttaaacg tgggggtggg gcgcgtgatc
    42841 tgcatgtcga gcagataaaa ggcgaaccag actttctcgt tgtttatatt catgtcgata
    42901 cccaggaagc catgggtgcc aatatgctca acaccatgct ggaagccttg aaaccagtct
    42961 tagaagaact cagtcaggga cagagtctca tgggaatcct gtccaactac gcgaccgatt
    43021 ctctggtgac tgcaagctgt cgcatcgcct ttcgctactt gagccgccaa aaggatcaag
    43081 gacgagagat tgcggagaaa attgcgttgg ctagtcagtt tgcgcaggct gatccttacc
    43141 gagctgctac tcataataaa ggaattttta atggtattga tgcgattttg attgccactg
    43201 gtaatgactg gcgtgccatc gaagctgggg cccatgcctt tgccagtcga gatggacgtt
    43261 atcaaggtct tagttgctgg acgctggacc ttgaaagaga agaattggtc ggtgagatga
    43321 ccctgcccat gcctgtagcg actaagggtg gctctatcgg cctcaaccca cgtgtagctc
    43381 tcagtcatga tctactagga aatccttctg ccagagaatt agcccagatt atcgtgtcca
    43441 tcggtcttgc ccaaaatttt gcagccctca aagctttggt aagtacgggc atccagcaag
    43501 gccacatgaa actacaggcc aaatccctag ctctcctagc tggggctagt gaatctgaag
    43561 ttgctcccct agtagagcgc ctcatctcag ataaaacctt taacctagag acagcccagc
    43621 gctatctcga aaatttaaga tcataaaaaa ctcagacgaa tcggtctgag ttttcttgtg
    43681 tttaaatact ttttcctggt aatagcgtaa ctggtaagaa gtaaccagtt gtggcgactt
    43741 ccttgccttc aatcttttgc aagagaagat caatagtgag acaagcaatc tcttccaaag
    43801 gttgcttgat agtagccaat tgagggtagt aattttcgat aaagtaggtc ccatcatagc
    43861 cgatgacctt gagctctttt gggacagaaa tgcccaattc ttgagcgatt ttaatgacca
    43921 gaatagctgt caaatcatcc gaagcaaaaa tggcatctgg tttttcccgg gtcaagatat
    43981 tcttgatttc catttctttt ctgacgggag aaaagtcact ggaaacattg ataataggag
    44041 cttttgggag tacggatgca aaaccagcgt ggcgcagtcc ggttggcgaa ttggaattgt
    44101 cattccctgt aatcatgatg atagactggg cacctgtctt gaccaaggtt tgggcagcaa
    44161 gaaccccacc agcatagttg tcagaggaga cgacagggat gtctggcgat aggtttcggt
    44221 caaaggaaat aatcggcgct gtcacacgat tgtagtcttc gattcctagg ttgtgactac
    44281 cagaaatgat gccgtccacc tgattggctt ccaacatttc gatgtattcg cgttccttct
    44341 cagaatcatg ttcactgttg cagatgatgg tcttgtaacc atttttgaag agttggtgtt
    44401 ccaatttatc aatcaattct gcatagaaaa cattggaaat attggggaaa atcaagccga
    44461 ttaacttagc tgattttcct tgcagactac gagccaggtt gttgggttta tagcccaatt
    44521 ctctcatggc ttcattgact ttttggatgg ttttctcaga tagataccct tttttattga
    44581 taacccgaga aacggtagta ggactgacgc ctgcaagttt ggcgacatca gttagttttg
    44641 cgaccataat ctaattcata gtaagttcca gttgggtttc cagatttgat caggatacca
    44701 ttttggtccg catgtgggaa gacacgacca gaaaatactt tttctccttt attgatgaaa
    44761 atttcaaaga cagagttatc gatgaagatt gtagcagtag tagcctgatt ctcgataggg
    44821 caagaacgag ttgtcccaaa ttcttgggca tactgttctc cagcctggct acgatccact
    44881 gtcacttgac cgtttacaag gtcaaagttg attgaaagtc ccttaccttc tttatcagca
    44941 agtaagacaa tctcgctctg gctattagct tccaagttga gttcaagttc gtaagtgttc
    45001 ttggtttggg aacggtttga gaaggcttct tcagaagcac gaaggtcctt aatagcagcg
    45061 actggatact ggtagagctt gtcgtctttg atagtgagtt ccttgaccaa agagaaggtt
    45121 ccttggtggt caaaacggtc agatgggtaa gaaacatctg gcaaaccaag ccagctaact
    45181 gctagagcac gcccatcagg agcgttgaag gcttgagttg cataggcttc gaaaccgtaa
    45241 tccatgtttt gaagttgaga cacatctacc attttggcat ttttagggtc aaaggaagcc
    45301 ccaatcttat acatatttgg aaagatatta tcgtagtcta gaactttctt atccaatcct
    45361 tgtggacagt agagaaggac aggctgttcc tctacaaaga ccagattagg acattccatc
    45421 atgtaggcag tacggtcgtt agcaaagtca aggtcgccaa ctgcttgcca gtttgtgtag
    45481 tcgttattga cagccttgta gagacggacg aagccttttt tctccaagtc ttgtccgccg
    45541 acaatggcat aatattgacc ctgaaagtta aaaatttgtg gatcgcggaa gtggtcagta
    45601 gagtctgctg gctggtcaat caagatcttg tcaatctttg taatcttacc ctccttgtcc
    45661 atcaaagcac cgatctggta tgggtgacgg atccagtttt tatcgcgaac atttcctgta
    45721 taaaatagga ataagttatc gccaaattgc atggcagaac cagagtaggc accgtggcta
    45781 tctaatggag tatctggtaa aactttgact ccagtttctc taaagtgaat caaatcatca
    45841 ctttctagct gtgcccaaga ttttaaaccg tgggctgcac caaaaggaaa attctggtaa
    45901 aagaggatcc acttgccatc aaagtaagaa aagccatttg ggtcgttgag aagtcctgtt
    45961 tttggctcaa catggtaatg agtatgccat ggagattgtg ccatattttc ctttatatgt
    46021 tgaatttctt cttgcgtcca atcttgataa agtctgtaac gacgctcagt tgtccattcc
    46081 attcattcct ccaaaaatct tatcactttt aatagtaact gttttcgata aaaaatgcaa
    46141 gccttttata ttaaaaaaga gaaaaaactg tcaaacgatt atcataaaat aaatacagga
    46201 aatcaatcaa atttttacga aaatgtgaaa gataatgaaa taaaagactt tttaaccaag
    46261 gtttcaaatt tatttttcaa ggaattcctg atgaaacagt caaaaacaat caatctaaag
    46321 cttgcccact aagtggatag aatatttttc tgcaaaacgc ttgacatatt tttaaaacat
    46381 gataaaataa tagtcgtaag ggcgaataaa atcgctttca aacaagaaca aaatgttata
    46441 taaggagatt tttgcaaatg aacaatcagg aaattgcaaa aaaagtcatc gatgccttgg
    46501 gcggacgtga aaatgtcaat agtgttgccc actgtgcgac tcgtctacgt gtcatggtca
    46561 aagatgaaga gaaaatcaat aaagaagtga ttgagaactt ggaaaaagtt caaggtgctt
    46621 tctttaactc agggcaatac caaattatct ttggtacagg tacagttaac aaaatgtacg
    46681 atgaagttgt tgtacttggc ttaccaacat catctaagga tgacatgaaa gcagaagttg
    46741 ctaaacaagg gaactggttc caacgtgcta tccgtacttt cggtgatgtt ttcgttccaa
    46801 tcatcccagt tatcgtagcg acaggtctct tcatgggtgt gcgtggtctt ttcaacgctc
    46861 ttgaaatgcc acttccaggt gactttgcaa cttacacaca aatcttgaca gatacagcct
    46921 tcatcatctt gccaggtttg gttgtgtggt caaccttccg tgtatttggt ggaaatcctg
    46981 ccgttggtat cgttcttggt atgatgcttg tctctggctc acttccaaac gcttgggcag
    47041 ttgctcaagg tggtgaagta acagcgatga acttctttgg tttcatccct gttgttggtt
    47101 tgcaaggttc cgttcttcca gccttcatca tcggggttgt cggagctaaa tttgaaaaag
    47161 ctgtccgcaa ggttgttcca gatgtcattg acctcttggt aacaccattc gtgacacttt
    47221 tggtcatgtc tatccttgga ctctttgtca ttggaccagt tttccacgtt gttgaaaact
    47281 acatccttat tgctacaaaa gcgattctta gcattccatt tggtcttggt ggtttcttga
    47341 ttggtggggt tcaccaattg atcgtcgtgt caggtgtgca ccacatcttc aacttgcttg
    47401 aagtgcaatt acttgctgct gaccatgcta acccattcaa cgctatcatc acagctgcta
    47461 tgacagctca aggtgctgct actgttgcgg ttggtgttaa aacaaaaaat ccaaaactga
    47521 aaacacttgc tttcccggct gctctttctg ccttcctagg tattacagag cctgctatct
    47581 tcggggtgaa cttgcgcttc cgtaaaccat tcttcctttc attgattgct ggtgcaatcg
    47641 gtggtggatt ggcttctatc cttggacttg ctggtactgg taatggtatc accatcatcc
    47701 ctggtacaat gctttatgtt ggtaacggac aacttccaca ataccttctt atggtagctg
    47761 tatcatttgc ccttggtttt gctcttactt acatgtttgg ttacgaagat gaagtagacg
    47821 caactgcagc tgcaaaacaa gctgaagtgg ctgaagaaaa agaagaagtt gcgccagcag
    47881 ctcttcaaaa tgaaacactt gtaactccta tcgtcggtga tgttgtcgct cttgctgatg
    47941 tcaatgaccc agtcttctca agtggagcta tgggacaagg tatcgctgtg aaaccaagcc
    48001 aaggcgtggt ctatgcacca gctgatgctg aagtttcaat tgcctttcca acagggcacg
    48061 cttttggttt gaaaacaaga aatggtgctg aagttttgat tcacgttggt attgacactg
    48121 tatctatgaa cggtgacggt tttgaagcaa aagttgctca aggtaataag gtgaaagctg
    48181 gcgatgttct tggaacattt gactcaaaca aaatcgctgc agctggactt gatgatacaa
    48241 caatggttat cgttacaaat acagctgact acgcttcagt agctccagtc gcaacaggtt
    48301 cagttgcgaa gggggatgct gtgatcgaag tgaaaatcta atcaatcctc tctaatgtga
    48361 aaacgaacaa atgacatgtt tgttcgtttt tattagatag taagatttac agaggaaaat
    48421 ctaaaaaata gagacattta gactttcgaa gtatgctata ataaagaaaa taaaaacaag
    48481 aggtttatca tgacaaaatt atatggaagc ttggaagcgg gcggtacaaa gtttgtctgt
    48541 gctgtcggtg atgaaaactt taacgtcgta gaaaaaacac aatttccaac aacaactcca
    48601 atcgaaacaa tcgataaaac cattgagttc ttctcaaaat tcgataacct tgctggtctt
    48661 gcagttggtt catttgggcc gattgatatt gacaaaaact caaaaactta tggctttatc
    48721 acgacgactc caaaaccaaa ctgggcaaat gtggacttgc ttggtgccct tcgtcgcgcc
    48781 ctaaacgtgc caatgtactt cacaacagac gtaaacagct ctgcttacgg tgaagtggtt
    48841 gcccgtaaca atgctggtgg tcgtatcgaa aacttggttt actacacaat cggtacaggt
    48901 atcggtgcag gtgtcctcca acgtggtgag tttatcggtg gtgtgggtca ccctgaaatg
    48961 ggtcattatt atgttgctag acacccaatg gatattgaaa aagagtttaa gggtgtttgt
    49021 cctttccata agggatgtct agaaggttat gcagctggtc caagtttgga agctcgtaca
    49081 ggtgtacgtg gggaaactat tgaattcaac aaccctgttt gggatgttca agcctactat
    49141 atcgctcaag ctgcggttaa tgcgacagtg actttccgcc cagacgtgat tgtctttggt
    49201 ggaggggtca tggctcaaca acatatgctg gaccgtgtcc gtgagaaatt tacatctctt
    49261 cttaatggtt acctaccagt accagatgtg cgtgactata tcgtgacgcc agcagtcgca
    49321 ggaaatggtt ctgccacact tgggaacttt gttcttgcaa aagaagtttc aaaataaagc
    49381 acaaaatccg cttgggaaac caaacggatt ttgtggttga aaacaatatt tttgagcctt
    49441 tccttaagtg attacggacg gtagcgactt tcttcgaaat tccataccta aactttgaga
    49501 cactaggctc aaagtttccg aatacctgaa accattagat ttcaggtgtt tctatcacgg
    49561 cgggaaggct aggtaagtcc tcgcctcact atcgaaaaat atgcatgatt tccataccaa
    49621 agtatattta gtatcaagct atagttaatc tacgctttaa aaatccactt gggaaaccaa
    49681 acggattttt tacgtgtcaa agcatttgcc agaaaaagtc aataatatag gtcagaccgt
    49741 aggtataaac cacgagggta aagggtttgg tcagaatgat gatagtcatg tagcgcttga
    49801 aactcatctt ggtcagggca gccagcatac agagaaagtc agctgggcta atgggccaaa
    49861 tcatcataaa aataaagaag cggtcaaaac gattgccctt atctagccag ccgatgtact
    49921 tgtcgtaggt gcgcttgctg acgacagact ggacaaaggc agctccgtat aggcgcacca
    49981 gataaaagat aatggcacag ccaatcacga tgccgatata gttgtagata gtcccgatga
    50041 tgtgcccgta gataaagacc ccagccaccg aggtcaaggc ccctggaatg atagggacga
    50101 cagtctgtaa aatctgtaaa aagataaaga gaggtggacc ccagatgcct gcctgctgga
    50161 taaaggcaga gagggtttcc ttggattgta aaatcccagc ctgataagcc caaatacaga
    50221 aaatcaaact cccaacccca ccgacaatcg atgagatatt gataactcgc tgcagaaggg
    50281 gagaaacagc tttcatttgt ttatcctgtg acatgtaaac tcctcataga tagtatattt
    50341 ctactatacc atattttgga ggagaaaggg ggaatgagtc ttttttgata aggaatagaa
    50401 agtaaatagc ccttcgagac ttagaatagg tctgtcaact aaaggttata tgcattctag
    50461 gtttcgaata atcgcaattc cataaattta aaagcattct aagtttcgaa tagcttatct
    50521 gagataattt tgaggcattc gcgctttcga atagtcattt tccctaaagt ttagatgtat
    50581 tctgaagcta gaaagactaa aatctaaaag tagtaagagt ctctctctga tggacgtaat
    50641 ttatggctag tatgaatcct ttgaattgtt agttaatttt aatattaatc agttctgaaa
    50701 taattactaa atatagttag atggtgaatt aattattgct aaatttcaag ttcaagttag
    50761 tcatccagaa gtcttctctg ggggacttgt agttcaagca ttttttagga tagttgttaa
    50821 tccacttttc gatgaatgcg acttcctttg tagtcgtttt cttagttccc tgaggtaacc
    50881 atctatgaat gaacttgttg tgatttttat tagatcctat agtggtgtgc ataatagata
    50941 taatagattg aaactagaat agtacgcatc tacttctaaa acattgttag aaatcgattt
    51001 gactgtcccg atctatttgt cctgttttta tttcatttta ctatataatc cttagaaaat
    51061 atatgggaca agcggttgaa ttccgttccg tccattagta gataaaggat tactccttct
    51121 tgaaatgaca gatgaacttg tttcttataa aaatgttgtc tgaaactatt cgcttttttc
    51181 cagaagtttt agaggaagaa aactttatga ggaaaaagga gttactcaaa taacaattca
    51241 ggattaaaaa tagacagttg aggagcggaa ggtataaatt gagtttgcta ctgtataatg
    51301 gatttatcac taatactctt cgaaaatctc ttcaaaccac gttagcttcg ccttgccgta
    51361 gatatagtta ctgacttcgt cagttctatc cacaacctca aaacagtgtt ttgagctgac
    51421 ttcgtcagtt ttatctgcaa cctcaaagct gtactttgag cagcctacgg ctagcttcct
    51481 agttcgctct ttgcttttca ttgagtataa aagtgctcca ggccatattt tacacagcaa
    51541 tagtcttcta aaaatggaca aaaacattgt aaaggctatc aaaaaggagt ataatgagtg
    51601 caagaaattt cggaggtgaa agatgctaga agtaagaagt ctagagaaaa gttttggctc
    51661 caagcaagtt ttgtttggta ttgactttca agcgcgacca ggtcgtattt tgggactagt
    51721 cggaaaaaat ggtgctggaa aaacaacgat tttccatagt attttgaaat tcctagaata
    51781 tcagggagaa atcggtctgg atggtcagga tattcgtcag gagacctatg ctcggattgg
    51841 ttatctgcct gaagaacgca gtctcatgcc taaattgaca gttcttgaac aagttcgcta
    51901 cttggcgact ctaaaaggca tggatgccaa agaagtcaaa gaaaaactcc ctcaatggat
    51961 gaagaggttg aaagtgaaag gaaagctgac agataaaatc aagagtctgt caaaaggaaa
    52021 tcagcagaag attcagctca ttattactct gattcatgaa ccagacctga ttatcttgga
    52081 tgagcctttt agtggattgg acccagttaa tacagaattg ctcaaacaag tcatttttca
    52141 ggaaaaagag cgcggagcaa ccattatctt ttctgaccat gtcatgacca atgttgagga
    52201 actttgtgac gatattctga tgatccgaga tggccgtgtg gtcttgcacg gaccagttca
    52261 ggatgtccgc aatcaatacg ggaaaacgcg tctctttgtt tcaagtgaac gaagcaagga
    52321 agaattggaa aatcttcctc atgtcaaaca ggtgagcttg accaaacaag gcagttggaa
    52381 attgattttg gaggatgaga gcgctggaag ggaactcttc ccaatcttga ctcaaggtca
    52441 atatatcgca acatttgacc aacaagcgcc aactatcgat gaaatcttta aactagaatc
    52501 aggagtggaa gtatgagaaa tatgtgggtt gtaatcaagg aaacctatct tcgacatgtc
    52561 gagtcatgga gtttcttctt tatggtgatt tcgccgttcc tctttttagg aatctctgta
    52621 ggaattgggc atatccaagg ttcttctatg gctaaaaata ataaagtggc agtagtgaca
    52681 acagtgccat ctgtagcaga aggactgaag aatgtaaatg gtgttaactt cgactataaa
    52741 gacgaagcaa gtgccaaaga agcaattaaa gaagaaaaat taaaaggtta tttgaccatt
    52801 gatcaagaag atagtgttct aaaggcagtt tatcatggcg aaacatcgct tgaaaatgga
    52861 attaaatttg aggttacagg tacactcaat gaactgcaaa atcagcttaa tcgttcaact
    52921 gcttccttgt ctcaagagca ggaaaaacgc ttagcgcaga caattcaatt cacagaaaag
    52981 attgatgaag ccaaggaaaa taaaaagttt attcaaacaa ttgcagcagg tgccttagga
    53041 ttctttcttt atatgattct gattacctat gcgggtgtaa cagctcagga agttgccagt
    53101 gaaaaaggca ccaaaattat ggaagtcgtt ttttctagca taagggcaag tcactatttc
    53161 tatgcgcgga tgatggctct gtttctagtg attttaacgc atattgggat ctatgttgta
    53221 ggtggtctgg ctgccgtttt gctctttaaa gatttgccat tcttggctca gtctggtatt
    53281 ttggatcact tgggagatgc tatctcactg aataccttgc tctttatttt gatcagtctt
    53341 ttcatgtacg tagtcttggc agccttccta ggatctatgg tttctcgtcc tgaggactca
    53401 gggaaagcct tgtcgccttt gatgattttg attatgggtg gtttttttgg agtgacagct
    53461 ctaggtgcag ctggtgacaa tctcctcttg aagattggtt cttatattcc ctttatttcg
    53521 accttcttta tgccgtttcg aacgattaat gactatgcgg ggggagcaga agcatggatt
    53581 tcacttgcta ttacagtgat ttttgcggtg gtagcaacag gatttatcgg acgcatgtat
    53641 gctagtctcg ttcttcaaac ggatgattta gggatttgga aaacctttaa acgtgcctta
    53701 tcttataaat agaagagcct tgcgaaagcg aggtttttaa aggtgaaaag ggtggaatca
    53761 cgaacctaat tatgtacaca ttatgtaaac aagttgcttg aaaactcaat tgtatagtga
    53821 actgaatctg gaataggata atattacttc taaaacattc ttacaaattg atttgaattc
    53881 caaaatccca tcgttccgtt cttatttcat tttgcgatat agtgaaagga aataagtcag
    53941 ctcacaaaaa acgcataata tcaaggattt tttgaactgc cccccaaaag ttagattttt
    54001 tctgtctaac ttttgggggg cagttcatat tcaacacttg atactatgcg ttttctaatt
    54061 ttaaagactt tttgcctggt ctcttttgtt ctatgagtat gttacatatc taggtaaaaa
    54121 ttatttaaag caaattaacc ttatttctca agatgtagta ggttccgaga taaaagatag
    54181 ctatgaatat gagttcttca acaatggcta tacctgctcc catatagaaa tggtcattga
    54241 gtcctaccag tgaattgaca tagaaattag aactaagatt gaagaaaagt tcaataaatc
    54301 caatgacgat ttggatacca atgtagactg caacagcgag tgctgtacgg tattcattga
    54361 aaagctgtcc aatggaaata gccaggtaga tgcagaggat tcctgaaatg gtatttagta
    54421 ggaaggatat acctgtaaga aagatctgag ggagatgtgt ttctacaaat gtaatcacat
    54481 aagaaagagg aatccattct ggagctgtta aagccagaat aataacagca cttagagcca
    54541 atacagcggt gctaatcaat gaccagataa aggcaccgat tagtttggct gtgatgatat
    54601 ggtgttcaga aactggcaag gtcaaagtca gatagccttg tcggtcgtag acactacctt
    54661 tgaagcgttt aataatcaag aaaatggttg aaatcgcaag tgtaagcatc aagccaccaa
    54721 agactgtagc tagaaaaagt agcatagtag cctgactttc ttggtaaggt agatttttaa
    54781 agccttgtgt ctgtattccg atgagggcag aaaggactag cacggctgcg tagagggcta
    54841 aataccactt gttaacattt ttaaattcgt agtgaactaa attccaaaac ataaataatc
    54901 tcctttgctt aggccttaaa ttcctgacgg aagagttggt caatggattc acctgactcg
    54961 tagcgaatat catctacatt tccttgacgg acgacttttc cgtcttttag gaagacaatt
    55021 tcatccaaga ttggctcgat atcagaaatc aagtgggtag aaatcaaaac ggtagaagtt
    55081 ggtgagtagt tgttgataat ggtattgagg atataagcac gggctgctgg atccacccca
    55141 ccaatgggtt catccaaaac atagagacga gcatcacggc tcataaccaa aatcagttga
    55201 accttttctt tgtttccttt tgatagtttc ttgagacgac tattttcatc aatgcccagg
    55261 tctgcaagta gatgatgggc gcgttcaaga ttgaaatctt tatagaaggt cttgaagtag
    55321 gttagggctt ctttgacctt catttgctca ttgagatagg tcgtatcagg caaataagct
    55381 acaacggcct tggttgctgg gcttgggtcc atgtcgttga tgaggacacg tccttgatct
    55441 ggttgtaaga ggccattaat tagtttaatc agggttgttt ttcctgagcc gtttggccca
    55501 agaaggccga caatttttcc agctggaatg tcaagagaaa cattttcaag ggctggtgtt
    55561 gctccatatg atttggatac attttcaaat gctagtaatg acataggctt aaactccttt
    55621 aatataatca ctgactacgc ctggtagttc ttctttttca tagccaaaat gggtcatgga
    55681 ggaaacgaag tgttccaatt cttcttctga taattgtttg cgtgactggg cgattagctc
    55741 cttatcctta gtcacaaatc gtccagttgt tcgcttgctg tagacaaatc cttctcgttc
    55801 aaggtctgat aaggctcttt ggatggtatt gggattgaca ccagcctcgc tagctagctc
    55861 cctcacggtt ggaagttgtt gattgggttc cagtgtatgg gaaacaatct gaagcttgat
    55921 tttttccata atctgtaaat agatgggttt tttgttgtca aatgtccagg acatcttggt
    55981 ctcctttctc ttaccctttt gtcttaatta aataatacaa caaaacaaaa aacttgtcaa
    56041 gcaaaaagaa gaattgtttt gggaatcgct taaaaaatgg tataatgaaa agaaaacgat
    56101 aaggagggaa aggatgcgtt gtccaaaatg tggggctacc aagtcaagtg ttatcgatag
    56161 tcgccaagca gaagaaggga acaccattcg tagaagacgt gagtgcgacg aatgccaaca
    56221 ccgttttaca acctacgaac gagtagaaga aagaacctta gtggttgtta aaaaagatgg
    56281 cacacgggaa caattctcca gagataaaat ctttaatggg attatccgct cagcccagaa
    56341 acgtcctgtg tcaagtgatg aaatcaacat ggtagtcaat cgtatcgaac agaaactccg
    56401 tggtcgaaat gaaaatgaaa ttcaaagtga ggacattggt tcactcgtca tggaggagtt
    56461 ggctgaattg gacgagatta cctatgtacg ttttgctagt gtctatcgta gttttaaaga
    56521 tgtcagtgag ttagagagct tgctccaaca aatcacccag tcctctaaaa agaaaaagga
    56581 aagataaatg aagccaattg accgtttttc ttatctaaag aataatcggg tgtcgcaaga
    56641 tacctcatct ctggtacagt gctacctccc gattatcggt caggaggcac tgagccttta
    56701 tctttatacg attagttttt gggataatgg tagaaaggaa tatctttttt caagtatcct
    56761 caatcatctt aactttggaa tggatagact gataaaatca ttgaaaatct tatctgcttt
    56821 taatctcttg actctctatc aaaaggggga tgtttatcag ctagccctcc atgctcctct
    56881 atctagtcaa gacttcttgg ggcatcctgt ttatcgcaga ctcttagaga aaaagattgg
    56941 ggactctgct gtggaggatt tgaaagttga aagtgctgat ggtgaagaaa tacctgtctc
    57001 actcaatcaa gtctttccag aattggcaga actaggcagt caagaagacc ttggtctcaa
    57061 gaagaaagtg gccaatgatt ttgacttgga acattttcgc cagctgatgg ctcgagatgg
    57121 acttcgtttt gcggatgagc agtccgatgt cttaaacctc tttgctattg ccgaggagaa
    57181 gaaatggact tggtttgaaa cctatcaatt ggccaagtca acagctgttt ctcaggttat
    57241 ttcaaccaaa cgcatgcgtg aaaaaattgc tcaaaaacca gtttcctctg actttagtct
    57301 taaggaaaca accattatca aagaagccaa aagtaaaact gccttgcagt tcttggcaga
    57361 aatcaagcaa acacgcaagg gaaccattac ccaaacagaa agagaactct tgcaacagat
    57421 ggctggcttg ggcttgctgg acgaagtcat caatatcatt cttttattga cctttaataa
    57481 ggtcgattcg gcaaatatca atgagaaata tgccatgaag gtagccaatg actatgccta
    57541 tcaaaagatt cattcggcag aaaaggcagt cttgcgcatc cgtgagcgag aacagaaagc
    57601 aaaaacacaa aaacagaatc agactgcccc agcaaaaacc aatattccta aatggagtaa
    57661 tcctgaatat aaaaatacgt catccgctga ggaattagaa gagatggaac gccaaaccct
    57721 agaattatta gcaaaattag ataacggagg tgattagatg gaaagtgtcg gagatgtact
    57781 caaacgtcaa ccaagtcgtt ttcactatca agatttagtt cagaaaatca tgaaggatcc
    57841 tgatgttgcg gcctttgtcc agcaagaatc cctcaatcag gacgagttga accgtagtat
    57901 ttccaagttt aaccaataca tcactgagag agataaattc ctccgaggag atacagatta
    57961 tattgccaaa ggctacaagc ctatattagt catgaaccat ggctatgcgg atgtttctta
    58021 tgaagaaact cctgaactaa tcgcagctga aaaagaagcg gctatcaaga aacgtctcaa
    58081 cttaattaat tttccatcta gcctgaaaaa tgtcagtttt ttagatgttt atcgtgatga
    58141 tgttcagcgc ttaactgttc taaaaagaat gatagaattt gttaacgatt accccaataa
    58201 tttgaaaggt ctttacttgt atggagactt tggtgtgggt aaaagtttca tggtggctgc
    58261 cctagctcat gatttatcag aaaaacgtgg tgtatcatcc actctcctcc actatcctag
    58321 ctttgtcatt gatgtcaaaa atgctatcag tgatggcaat gttaagacct tggtggatga
    58381 gattaagctg tctgatgtcc tgattttaga tgatattggc gccgagcaat caaccgtttg
    58441 ggtgcgtgac gaaatcctgc aggtcattct ccaatatcgg atgcaggaaa atttaccgac
    58501 ctttttcaca tccaacttca actttgaaga tttggagaag cattttgcga aagtgaaaca
    58561 tggaaatgac gaaacctggg aagccagacg ggtcatggaa cgcatccgtt atttggctga
    58621 ggagactcgt ttagaaggag taaaccgtcg atgacagaaa ccattaaatt gatgaaggct
    58681 catacttcag tgcgcaggtt taaagagcaa gaaattcccc aagtagactt aaatgagatt
    58741 ttgacagcag cccagatggc atcatcttgg aagaatttcc aatcctactc tgtgattgtg
    58801 gtacgaagtc aagagaagaa agatgccttg tatgaattgg tacctcaaga agccattcgc
    58861 cagtctgctg ttttccttct ctttgtcgga gatttgaacc gagcagaaaa gggagcccga
    58921 cttcataccg acaccttcca accccaaggt gtggaaggtc tcttgattag ttcggtcgat
    58981 gcagctcttg ctggacaaaa cgccttgttg gcagctgaaa gcttgggcta tggtggtgtg
    59041 attatcggtt tggttcgata caagtctgaa gaagtggcag agctctttaa cctacctgac
    59101 tacacctatt ctgtctttgg gatggcactg ggtgtgccaa atcaacatca tgatatgaaa
    59161 ccgagactgc cactagagaa tgttgtcttt gaggaagaat accaagaaca gtcaactgag
    59221 gcaatccaag cttatgaccg tgttcaggct gactatgctg gggcgcgtgc gaccacaagc
    59281 tggagtcagc gcctagcaga acagtttggt caagctgaac caagctcaac tagaaaaaat
    59341 cttgaacaga agaaattatt gtagaaagtg agaaattatg gccctaccaa ctattgccat
    59401 tgtaggacgt cccaatgttg ggaaatcaac cctatttaat cggatcgctg gtgagcgaat
    59461 ctccattgta gaagatgtcg aaggagtgac acgtgaccgt atttatgcaa cgggtgagtg
    59521 gctcaatcgt tcttttagca tgattgatac aggaggaatt gatgatgtcg atgctccttt
    59581 catggaacaa atcaagcacc aggcagaaat tgccatggaa gaagcagatg ttatcgtctt
    59641 tgtcgtgtcc ggtaaggaag gaattaccga tgcagacgaa tacgtagccc gtaagcttta
    59701 taagacccac aaaccagtta tcctcgcagt taacaaggta gacaaccctg agatgcgaaa
    59761 tgatatatat gatttctatg ctctcggttt gggtgaacca ctgcctatct catctgtcca
    59821 tggtatcggt acaggggatg tactagatgc gattgtggaa aaccttccaa atgaatatga
    59881 agaagaaaat ccagatgtca ttaagtttag cctgattggt cgtccaaacg ttggaaaatc
    59941 aagcttgatc aatgctatct tgggcgaaga ccgtgttatt gctagtcctg ttgctggaac
    60001 aactcgtgat gccattgata cccactttac agatacagat ggtcaagagt ttaccatgat
    60061 tgatacggct ggtatgcgta agtctggtaa ggtttatgaa aatactgaga aatactctgt
    60121 tatgcgtgcc atgcgtgcta ttgaccgttc agatgtggtc ttgatggtca tcaatgcgga
    60181 agaaggcatt cgtgagtacg acaagcgtat cgcaggattt gcccatgaag ctggtaaagg
    60241 gatgattatc gtggtcaaca agtgggatac gcttgaaaaa gataaccaca ctatgaaaaa
    60301 ctgggaagaa gatatccgtg agcagttcca atacctgcct tacgcaccga ttatctttgt
    60361 atccgctttg accaagcaac gtctccacaa acttcctgag atgattaagc aaatcagcga
    60421 aagtcaaaat acacgtattc catcagctgt cttgaacgat gtcatcatgg atgccattgc
    60481 catcaaccca acaccgacag acaaaggaaa acgtctcaag attttctatg cgacccaagt
    60541 ggcaaccaaa ccaccaacct ttgtcatctt tgtcaatgaa gaagaactca tgcacttttc
    60601 ttacctgcgt ttcttggaaa atcaaatccg caaggccttt gtttttgagg gaacaccgat
    60661 tcatctcatc gcaagaaaac gcaaataaaa aagtagaatc tggaatgaca gttccggatt
    60721 tttttgatat aataaaataa tagaaaacgc tatcaaaaga aagagggtga tgtcggtgat
    60781 ggaacatttg tttaaattct tacttttagc accgtacttt tattttgata actggattga
    60841 gaaggccaac agaaatagta agttttttcc aattttttat tatttttact ggttttacat
    60901 ccccttctat tctcttttta gccttgcttg gacagttgtt tcagttctgt ttttcaatac
    60961 cgtcttgaga aatgtgacag atatcaagtt atggggcatt tggtttcttt ttattctgct
    61021 agctattggt atgaattggt taacttattc ctgtttcaaa gaaatgtttc gcttgagaca
    61081 ggaactaggg aagtctaagg gtggaaggca ttgatttata tcggtttcta tttgtaggag
    61141 gtggccttat ggtacctcta aaatcattta ttacacgcta ttccaaggtt tatattggta
    61201 ataacattta atagaacgaa actaaagaaa acaatttaaa agaggaggtt tgtgatggaa
    61261 cacttgttta aaatgataat cttattaccc tgtttttact ttttcagctg gattgacaag
    61321 gataatagag aaagtaaatt tttcccaatt tttcccaatt ttttactatt tttactggat
    61381 ttacatcacc ctctatgctc tttttagcct tgcttggaca gttttttcag ttctgttttt
    61441 caatatcgtc ttgagaaatt tgacagatat caagttatgg ggtatttggc tattattact
    61501 acttatcgct tttgctagtg attggttagc ctatgtcttt ttcaaaaaaa tgcttgactt
    61561 gagacgggaa ctagggaagt ctaaaggcgg aaggcattga tttatactct tcgaaaatct
    61621 cttcaaacca cgtcaacgtc gccttggatt atatatgtaa ctgacttcgt cagtcttatc
    61681 tacaacctca aagcagtgct ttgagcaacc tgcggctagt ttcctagttt gctctttgat
    61741 tttcattgag tattatatta ctttctattt gtaggaggtg gcttatgaag attcctctct
    61801 taactttggc aaggcataaa tttgtttatg tcttgcttac tttgcttttt cttgctttgg
    61861 tttatcgtga cgttttgatg acttatttct tttttgatat tcatgcgccc gatctagcta
    61921 aattcgatgg acaagcaatt aaaaatgact tattaaaatc agcattagat tttcgtattc
    61981 tccagttcaa tctaggtttt tatcaatcat ttattattcc aatcatcatt gttttgctag
    62041 gttttcaata tattgagctg aaaaataaag ttttacgatt gagtattgga agagaagtga
    62101 gctatcaagg gttaaaaaga aagttgactt tgcaagttgc aagtatccct tgtttgatat
    62161 atttagtgac tgtgctgaca attgcaatta taacctattt ccttgggact ttttctcctc
    62221 ttggatggaa ttctctattt tctgatggaa gtggtttaca aagactccta gatggagaga
    62281 taaaaagcta tttgttcttt acttgtgtcc tactaatcgg tattttcatc aatgcaatct
    62341 attttttaca aatagttgat tatgtgggga atgtgactcg ttcggcaatc acctatttga
    62401 tgtttctttg gcttggttct atgctgctct atagtgcctt gccttactat atggttccta
    62461 tgacgagttt gatgcaagct agctatgggg atgtaagttt gatgaaactc tttactcctt
    62521 atatccttta tattgtccct tatatggtgc ttgaaaaata tgaagataat gtttaagaat
    62581 tttaacaata tttggctaaa gagaaagatc gttttactac ttcgtatagt tctgatgatg
    62641 attttgataa actatctatt gtcaacagcg gttcaaaagc aggatgctgt tatctttttc
    62701 aagagagaat tgatttcaat cttttcctat aatgactatt ctgaagcgaa tttagaaatc
    62761 cccaaactct tgttaaacct ttcgcttttc atggtaggat ggctctctgt cattttactt
    62821 gaaagtgatt tggcagacca ttaccatcac ttgattcgct atcaatcaag ctcctttttc
    62881 gattatacaa ggaaacgatt ggttgtcatt tctaaatttt ttactcaaga tttgtttgtc
    62941 tggtttcttg gtttacttcc gctaggaatt catttcaaaa cagtcgcact tttcttttta
    63001 cttgctcagt taatgatgtt gtacttacta ctgtcttatc tgatagcact gattagtgcg
    63061 ggcgctggtt tttctttttt tctctatttt ttggcatttg tgggacaaga atggatgatg
    63121 gatcatattg taacagtgta tttactactc ttaagtttat tagttatgtt gattgttagt
    63181 cgcttggaag agaaatttaa gaaaggataa acgatgagac ttgaaattat aaatggacag
    63241 aaaatttatg ggaaaagacc tattttaaat cagttgaatt tggtgtttca atcaggaaaa
    63301 atttatggac ttaaaggtga taatggatct ggcaagacgg ttcttttaaa gatacttgct
    63361 ggttatatta agcttgacaa aggaaaagtt cttcaagatg gtaaagttta cggggtaaaa
    63421 aatcattata ttcaggatgc aggaatttta attgaaaaag tcgagttttt atctcattta
    63481 tccctgagag aaaatttgga actgttaagg tatttttcat ctaaagttac ggaaaaaaga
    63541 attgcctatt ggattcaata ctatgattta caggaatttg aagacattga ataccgtcat
    63601 ttatccttag gaacaaagca aaaaatggcc ttgattcaag cctttatttc ctctccttct
    63661 atactctttc tcgatgaacc tatgaatgct ttggatgaga agagtgtgag gttaaccaaa
    63721 caggtcattt tatcttacct gaaaaaagaa aatggtctgg ttatcctgac gtcgcacata
    63781 tcggaagata tttcagacct ttgtacagat gtattagttg tcgaaaatgg acatatacaa
    63841 atgtaaagga tatacaatcc taggagatgg cttatggcac atctaaaatc atttattaca
    63901 cgctattcta aggtctatat tggtttagtt ctgttgatct ggattgcttt cttctttctt
    63961 ccttggggta gtccgattct ggggggggag attgacctct tcagcataca gaaagtcttg
    64021 ctagtttttg gcattctgtc cattttgatg gccttgctgt ccaagaaagt cagtctcttt
    64081 gtttttggat tgatctgctg tctttctctt tggattaact tatttatcct atttgctatt
    64141 ttgccgattc ttggcaatta aagtatcata aaagtcagag aggttagctt ggaaactaac
    64201 ctctttttcc ttttcaaaat ggggattctt ccttgaaaat aatcagtaat tgtgctaaaa
    64261 ttaaaggaac attctaaaat attcggaatt taaagtaagg aaaaacatgg ctaatatttt
    64321 aaaaacaatt atcgaaaatg ataaaggaga aatccgtcgt ctggaaaaga tggctgacaa
    64381 ggttttcaaa tacgaagacc aaatggctgc tttgactgac gaccaactaa aagcaaaaac
    64441 agttgaattt aaggaacgtt atcaaaatgg agaatcactg gattcattgc tttacgaagc
    64501 atttgcggtt gtccgtgaag gtgccaaacg tgtcctaggt ctcttccctt ataaggttca
    64561 ggtcatgggg gggattgttc ttcaccatgg tgacgtgcca gagatgcgta caggggaagg
    64621 gaaaaccttg actgcgacca tgccggtata cctcaatgcc ctttcaggta aaggggttca
    64681 cgtagttacg gttaatgaat acctgtcaga acgtgacgcg actgagatgg gtgaattgta
    64741 ctcttggctt ggtttgtcag tagggattaa cttggctacc aaatctccaa tggagaaaaa
    64801 agaagcctat gagtgtgata ttacttactc aactaactca gaaatcggat ttgactacct
    64861 tcgtgacaac atggtcgttc gcgctgaaaa catggtacaa cgtccgctta actatgcctt
    64921 ggtcgatgag gttgactcta tcttgattga cgaggctcgt acacctttga ttgtatcagg
    64981 tgccaatgcg gttgaaacca gtcagttgta tcacatggca gaccactatg taaaatcttt
    65041 gaacaaagat gactacatca tcgatgtgca gtctaagact attggtttgt ctgattcagg
    65101 gattgacagg gctgaaagct acttcaaact tgaaaacctc tatgacatcg aaaacgtggc
    65161 tttgacccac tttatcgata acgcccttcg tgccaactac atcatgcttc tcgatattga
    65221 ctatgtggtg agcgaagagc aagaaatctt gattgtcgac caatttacag gtcgtaccat
    65281 ggaaggtcgt cgttattctg atggattgca ccaagctatt gaagccaaag aaggtgtgcc
    65341 aatccaggat gaaaccaaga catctgcctc aatcacgtac caaaaccttt tccgtatgta
    65401 caagaaattg tctggtatga cgggtacagg taagactgag gaagaagaat tccgtgaaat
    65461 ctacaacatt cgtgttattc caatcccaac aaaccgtcct gttcaacgta ttgaccactc
    65521 agaccttctt tatgcaagta tagaatctaa gtttaaagcg gttgtcgaag acgttaaggc
    65581 tcgttaccaa aagggtcaac ctgtcttggt tggtacagta gcggttgaaa ctagtgacta
    65641 catttctaag aaattggttg cagctggtgt tcctcacgaa gtcttgaatg ccaaaaacca
    65701 ctatagagaa gcccaaatca tcatgaatgc tggtcaacgt ggtgccgtta ccatcgcaac
    65761 caacatggcg ggtcgtggta ccgacatcaa gcttggtgaa ggtgttcgtg aacttggagg
    65821 actttgtgtt attggtacag aacgtcatga aagtcgtcgt atcgataacc agcttcgtgg
    65881 acgttcaggt cgtcaaggag atccaggtga gtcacaattc tacctatctc ttgaagatga
    65941 tttgatgaaa cgttttggtt ctgaacgctt gaagggaatc tttgaacgct tgaacatgtc
    66001 tgaagaggcg attgagtctc gcatgttgac gcgtcaggtt gaagcagctc agaaacgtgt
    66061 cgaaggaaat aactacgata cccgtaaaca agtccttcaa tacgacgatg tcatgcgtga
    66121 acaacgtgag attatctata ctcaacgtta tgatgtcatt actgcagacc gtgacttggc
    66181 acctgaaatt caggcaatga tcaaacgcac gattggtcgt gtcgttgacg gtcatgcgcg
    66241 tgctaaacaa gatgagaaac tagaggcaat tttgaacttc gctaagtata acttgcttcc
    66301 tgaagattct attacgatgg aagacttgtc aggcttgtct gataaggcca tcaaggaaga
    66361 gcttttccaa cgtgccttga aggtttacga tagtcaggtt tcaaaactac gcgatgaaga
    66421 agcagttaaa gaattccaaa aagttttgat tctacgagtg gtggataaca agtggacaga
    66481 tcatatcgat gcccttgatc aattgcgtaa cgcggttgga cttcgtggct atgctcagaa
    66541 caaccctgtt gttgagtatc aggcagaagg tttccgtatg tttaatgata tgattggttc
    66601 gattgagttt gatgtgacac gcttgatgat gaaagcacaa attcatgaac aagaaagacc
    66661 acaggcagaa cgtcatatca gtacaacagc gactcgcaat atcgctgctc accaagcaag
    66721 tatgccagaa gatttggatt tgagccagat tggacgcaat gaactttgcc catgtggttc
    66781 tggtaagaaa tttaaaaact gtcacggtaa aagacaataa aatgagatag tttagaggcg
    66841 gatatcttgt gaaaagtaaa tttttactgg gtatccgttt gctttataag gagatgagtt
    66901 atggtattta cagcaaaaag ctctaaaata aatatagaag aagttcgtgc cttgtcaaaa
    66961 ttagaaggtc aggctttgga gaggaaatca cagcgagatc aagagctaga agccattata
    67021 cgtggagaag accagcgaat tctcttggta atcgggccat gctcatctga caacgaagaa
    67081 gctgtccttg aatacgctaa gcgtttggca gtcctacaag aagaagtggc agatcgtatc
    67141 tttatggtta tgcgtgttta tactgccaaa ccccgtacca acggagatgg ctataagggc
    67201 ttgattcacc agcctaacgc gacagaagcg cctagtctta tcaatggaat caaagccgtt
    67261 cgccatcttc actatcgtgt catcacagaa acagggatga caactgctga tgaaatgctt
    67321 tatcctgaaa accttccgct tgtagatgat ttgatttctt acatggcagt tggtgcccgt
    67381 tcagttgaag accagcaaca ccgctttgtg gcaagtgggg caggattttc tactggtttt
    67441 aaaaatccaa cctctggaaa tctcaatgtc atgtttaatg ggatttatgc tgctcaaaac
    67501 aaacaaagtt tccttttctt aggaaaagaa gtagaaacaa ctgggaaccc gctttcacac
    67561 gctattcttc gtggtgctct taatgagtat ggaaaaaata ttcccaacta ctattatgac
    67621 aatttaattg ataccattgc ccagtatgag aaaatgggct tggaaaatcc ttttatcatc
    67681 attgatacca atcatgacaa ttctggtaag cagtatattg aacagatccg aattgtccgc
    67741 cagaccttga ttaaccgtgc ttggaatgaa aaaattaagc agttcgttcg tggttttatg
    67801 attgagtctt atctggaaga tggtcgacaa aatgagccag aagtatttgg taagtctatc
    67861 acagaccctt gcctgggttg ggataacaca gaagctcttg tcagagaaat ttacaaaacg
    67921 ttaggagaat aagatggcat ttattgaaaa aggtcaagaa atcgatatgg aagtcatcaa
    67981 ggctgaaacc caattgtctg cggaagcctt gagactcaag gaaagccgtg acagggaatt
    68041 ggcagatatt atttcagggg aagatgaccg tattctcttg gtgattggtc cttgctcttc
    68101 tgataatgaa gaggcggtct tggaatatgc tcgccgttta tctgccttgc aaaagaaggt
    68161 agcggataag attttcatgg tcatgcgcgt gtatactgct aagcctcgta ccaatggaga
    68221 cggctataaa ggattggttc accagccaga tacttctaag gctccaagcc tgattaacgg
    68281 cttgcaggct gtgcgccagt tgcactaccg cgtgattaca gagactggtt tgacaacggc
    68341 agatgagatg ctttatccgt caaatctgat cttggtggat gacttggtca gctaccatgc
    68401 cgttggagct cgttctgtgg aagaccagga gcaccgcttt gtggcttctg ggattgatgc
    68461 accagtaggg atgaaaaatc caacctcagg aaatttgggt gttatgttta acgccatcta
    68521 tgctgctcaa aacaagcaaa ccttccttta tcatgggcag gaagttgaga catcaggtaa
    68581 tcctttggcc catgttatcc tccgtggagc agtcaacgag tatggcaatt atatgccgaa
    68641 ttactactat gaaaacctac tccaagccat tgaacgctat gaaaccatgg gacttgaaaa
    68701 tccttttatc ctcattgaca ccaaccatga taactcaggc aagcaatata tggagcagat
    68761 tcgaattgtt cgccagacct tgcagaatcg tgattggaat gagaaaatta aaaagacggt
    68821 tcgaggattt atgattgaat cttacctagc agatggtcgt caaaaccaac cagagatctt
    68881 tggttgctct attactgacc cttgcctagg ttgggaaaat acagaggcct tggtagaaga
    68941 gatttatgtt accttgacaa aataagtgaa aaggatggag ttggggaatc tcaactcctt
    69001 ttgatgagaa tgatagttgg acacggaatt gacatcgaag aattggcttc gatagaaagc
    69061 gcagttacac gacatgaagg atttgctaag cgtgtactga ccgctcagga aatggagcgc
    69121 ttcaccagtc tcaaaggacg caggcaaata gaatatttag ctggtcgctg gtcggctaag
    69181 gaggcctttt ccaaggctat gggaacgggc attagcaagc tcggttttca ggatttggaa
    69241 gtcttgaaca atgaacgtgg ggcgccttat tttagtcagg caccattttc aggaaagatt
    69301 tggctgtcta tcagccacac cgatcagttt gtgatagcca gtgtcatttt ggaggaaaat
    69361 catgaaagct agtccacata gaccaaccaa ggctctgatt catctgggag ctattcgaca
    69421 aaatattcag caaatggggg ctcatatccc tcaaggaacg ctcaagttgg ctgtggtcaa
    69481 ggccaatgct tatggtcatg gagctgttgc cgttgccaag gcaattcaag atgatgttaa
    69541 tggcttttgc gtttccaata tcgatgaagc cattgaactc agacaagctg gactcagcaa
    69601 gccaatcctc attttaggag tttctgaaat cgaagctgtt gctctagcta aagaatatga
    69661 cttcaccttg acagtggctg gactggagtg gattcaagca ctcttagata aggaagtgga
    69721 cctaactgga ttgacagtcc acctcaagat tgattcaggg atgggacgga ttggttttag
    69781 agaggccagt gaggttgagc aggctcaaga tttgctccaa caacacggtg ttcgtgttga
    69841 aggaatcttt acccactttg ctactgctga tgaggaatca gatgactatt ttaatgccca
    69901 gttagaacgg tttaaaacta ttttagctag tatgaaggaa gttccagagc tggttcatgc
    69961 tagcaattct gcaacgactc tttggcatgt agagactatt ttcaatgcgg ttcgtatggg
    70021 agatgccatg tatggcctca atccaagtgg agcggtcttg gatttgcctt atgatttgat
    70081 accggccttg accttggagt ctgctctggt tcatatcaag acagttccag ctggtgcttg
    70141 catgggctat ggagcaactt atcaagcgga tagcgagcaa gtcatcgcga ccgtgccaat
    70201 cgggtatgca gatggatgga caagagacat gcaaaatttc tctgtcttgg tagatggcca
    70261 agcttgccca attgtcggca gggtttcgat ggaccaaatc actattcgat tgcctaagcc
    70321 ttacccactc ggaaccaagg taaccttgat tggctccaat ggggataagg aaatcactgc
    70381 aactcaggta gcgacctacc gcgtaaccat taactatgag gtggtttgcc tcctcagcga
    70441 ccgtattccg agagaatatt attagaaaag aaaggagtgg agcatgaatc tacatcaacc
    70501 cttgcatgtc ttgcctggtg tgggaccaaa gtcagcagaa aaatacgcca aactaggaat
    70561 tgaaaacttg caagatctct tgctctactt tcctttccgt tatgaagact tcaaaaccaa
    70621 gcaggtgctg gagctggaag acggtgagaa ggcagttctt tctggtcagg tagtgactcc
    70681 tgctagtgtc cagtattatg gtttcaagcg caatcgcctg cgttttagtc tcaagcaggg
    70741 agaggtcgtt tttgcggtga atttctttaa ccagccctat ctggctgata aaatagagtt
    70801 gggagcaacc cttgctgtct ttggaaaatg ggaccgcgct aaggctagtc tgactgggat
    70861 gaaggttctg gctcaggtag aagatgacct ccagcctgtc tatcgtctgg ctcagggaat
    70921 cagtcaggcc agtctggtca aggtcatcaa gacggctttt gatcagggac tggacctctt
    70981 gatagaagaa aatctgcccc agtctttact agacaaatac aaactcatgt cccgttgtca
    71041 ggcagtccga gctatgcatt ttccaaagga tttggcagaa tacaagcagg ctcttcgccg
    71101 tatcaagttt gaggaactct tttatttcca aatgcagctg cagacgctca agtctgaaaa
    71161 tagagttcag ggaagtggtc tggttctgga ttggtctcag gaaaaagtga cagcagttaa
    71221 agcaagtctt ccttttgccc tgacccaagc tcaggaaaag agtttgcagg aaattttaac
    71281 tgatatgaag tccgaccacc acatgaatcg tctcctacaa ggagatgtgg ggagtggaaa
    71341 aacggtagtc gctggcttgg ccatgtttgc ggcagtgaca gcaggttatc aggctgccct
    71401 aatggtacca acagaaatcc tcgcagagca acactttgag agtttacaga acctttttcc
    71461 caatttgaaa ctggctctct tgacaggttc cttgaaagct gcagaaaaga gagaagtctt
    71521 ggagaccatt gccaagggtg aggctgattt gattatagga actcacgctc tgatacaaga
    71581 tggggtggag tatgctcgtc ttggtttgat tattatcgat gagcagcacc gttttggtgt
    71641 agggcaaagg cgtattttac gggaaaaagg tgacaatcca gatgtcctca tgatgacggc
    71701 gactcccatt ccacggacgc ttgccatcac agcctttgga gatatggatg tttccattat
    71761 cgaccagatg ccagcaggtc ggaagcctat tgtgacgcgc tggatcaaac atgagcaact
    71821 acctcaggtc ttgacttggt tagaggggga aattcaaaaa ggttcccaag cctatgtcat
    71881 ctctcctttg attgaagaat cagaagctct agatttgaaa aatgccattg ccttatcaga
    71941 ggagttgacg actcattttg caggcaaggc agaggtggct cttctacatg gtaggatgaa
    72001 gagtgacgaa aaagaccaga tcatgcagga tttcaaggag agaaagacgg atattctggt
    72061 ttcgacgacg gttattgagg ttggggtcaa cgttcccaat gcgactgtca tgattatcat
    72121 ggatgccgat cgcttcggtc tcagtcaact tcaccagctt agaggtcgtg tcggtcgggg
    72181 ggacaagcag tcctacgctg ttctcgttgc caatcccaag acggattctg ggaaagaccg
    72241 catgcgcatc atgacagaaa cgaccaatgg atttgtcctt gcggaggaag atttgaaaat
    72301 gcgtggttct ggtgagattt ttggaaccag acagtcagga cttccagagt tccaagtggc
    72361 tgatattatc gaagattttc cgattttaga agaagcaaga aaggttgcta gctacattag
    72421 ttctatagaa gcttggcaag aagatccaga gtggcgcatg attgcccttc atctggaaaa
    72481 gaaagaacat ctggattaag ctttctctaa ggaaaactta agctagtttt taggtttggc
    72541 tcttatacta aagtcatcaa aaagaaacga ggactctcat atgacagtaa cgattaaagt
    72601 aaattaccaa accactttcc aaaagaagga agcaaaaaac tagtataaac agaagagaga
    72661 gcgaaatgct ctttttcgtt tctaaaacta ctttcagccc atcatcctaa aagtaaagaa
    72721 tctaaattca ctttctattt atccttcttt cttgcattga ttacatagat atgctacagt
    72781 tgtggtaacg attacaaaat aaaaggagca tgctatgaaa aatccagctt tgctagaaga
    72841 aattaagacc tatagaggaa gggatgaggt tccggaagac tttgatgctt tctgggatgg
    72901 ggaagtgaaa aatgtttcca cgcttccatc ctaccacttg gaggaaagag atttccacat
    72961 tcctcaagtc aagtgctatg agttaacatt tgaaggaagc aaggaaggaa aggtctatgc
    73021 acgcattgtt cttccaaaga gtgaggagaa ggttccacta atcttccatt ttcatggtta
    73081 tatgggacgt ggctgggact gggccgacat gctgggcttc accgtagctg gttacggtgt
    73141 tgtttccatg gatgtgcggg gccagtcagg ttactcacaa gacggcttgc gttctccttt
    73201 aggaaatacc gtgaaggggc atattatccg tggtgctatg gaaggtcggg accacctctt
    73261 ttataaggat gtttatctgg atatttacca gttggtcgaa attgttgcta gtctgtctca
    73321 ggttgatgag aagcgtcttt ctagctatgg tgcctcacaa ggaggggctc tagctctagt
    73381 tgcagcagcg ctcaatcctc gaattcagaa aacagttgcc atttatccct tcttgtcaga
    73441 cttcagacgg gtgattgaga ttggtaatac tagcgaggct tacgacgaac ttttccgtta
    73501 tttcaagttt cacgacccct tccatgaaac agaggaggaa atcatggcga cccttgccta
    73561 tatcgatgtc aaaaatcttg cccatcgtat ccaaggtgag gttaagatga ttacgggctt
    73621 ggacgacgat gtttgctatc ccattaccca gtttgcgatt tataatcgtc tgacctgcga
    73681 taaaacctat cgtatcatgc ctgagtatgc tcacgaaacc atgaatgtat ttgtcaatga
    73741 ccaagtctac aactggctct gtggaagtga gattcctttt aaatatctaa aataaggagt
    73801 cgactctaag cacaaaatct taaaaattac aaacacgcat agtatcaggg gattaagaaa
    73861 acttgatact atgcgtttta tcatggaaat atagtaaaat gaaacaagaa caggacaaat
    73921 cgatcaggac agtcaaatcg atttctaaca atgttttaga agtagatgtg tactattcta
    73981 gtttcaatct attatattta tagaattttt tgttgctaga tttgtcaaat tgcttaaaat
    74041 aatttttttc agaaagcaaa agccgatacc tatcgagtag ggtagttctt gctatcgtta
    74101 ggcttgtctg taggtgttaa tacttttcaa aaatctcttc aaaccacgtc agcttcgcct
    74161 tgccgtaggt atggttactg acttcgtcag ttctatccac aacctcaaaa c
//
LOCUS       DAVKWZ010000004        71144 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000004 DAVKWZ010000000
VERSION     DAVKWZ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 71144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71144
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.004"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            123..2015
                     /gene="pepO"
                     /locus_tag="VW854_000250"
     CDS             123..2015
                     /gene="pepO"
                     /locus_tag="VW854_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEV0261552.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
     gene            complement(2138..2767)
                     /locus_tag="VW854_000251"
     CDS             complement(2138..2767)
                     /locus_tag="VW854_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV0261553.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(3107..3349)
                     /locus_tag="VW854_000252"
     CDS             complement(3107..3349)
                     /locus_tag="VW854_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261554.1"
                     /translation="MMRFEEIFEEYKNPQDTFFYPLVYKENTYEKTAISIFALLMLGV
                     CCLFLFSQQSYKKLVQYYANDQNLPSRITYSEYSDK"
     gene            3760..5982
                     /locus_tag="VW854_000253"
     CDS             3760..5982
                     /locus_tag="VW854_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEV0261555.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRVIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            6012..6455
                     /locus_tag="VW854_000254"
     CDS             6012..6455
                     /locus_tag="VW854_000254"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEV0261556.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(6494..6908)
                     /locus_tag="VW854_000255"
                     /pseudo
     CDS             complement(6494..6908)
                     /locus_tag="VW854_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720030.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6927..7340)
                     /locus_tag="VW854_000256"
     CDS             complement(6927..7340)
                     /locus_tag="VW854_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261557.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFEWLLLSGLIFFLA
                     QGLLLFLLIVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMS
                     IITACHSTFYYMTADYLRENYPDFYDKHISLWECL"
     gene            complement(7442..8119)
                     /locus_tag="VW854_000257"
     CDS             complement(7442..8119)
                     /locus_tag="VW854_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEV0261558.1"
                     /translation="MVVGIAIASGYLNSRLDKFVDWGSWTALVPFGLISVTNVGISML
                     STRFTGKLSKWGNYFGIVNTLLFGAIDYILGNKAAIITYPVTFLIYTFAIKKWEASQE
                     GRPNQMSQKQVKLAAIIISIIAFLFAFVTNYIGYEGKMDLLAYVTTIAFALSLIANAL
                     NALKLTTQWGFWLIYNFVQLTKAGIQGNFANIGKYIFYILNAIGALFVWNDEEVRYIE
                     GNSNKEY"
     gene            complement(8318..8968)
                     /locus_tag="VW854_000258"
     CDS             complement(8318..8968)
                     /locus_tag="VW854_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEV0261559.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     NFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            9080..9922
                     /locus_tag="VW854_000259"
     CDS             9080..9922
                     /locus_tag="VW854_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV0261560.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQIGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            9998..10435
                     /locus_tag="VW854_000260"
     CDS             9998..10435
                     /locus_tag="VW854_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEV0261561.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            10503..10805
                     /locus_tag="VW854_000261"
     CDS             10503..10805
                     /locus_tag="VW854_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261562.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(10896..11969)
                     /locus_tag="VW854_000262"
     CDS             complement(10896..11969)
                     /locus_tag="VW854_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEV0261563.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDKLAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILEAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            12125..12802
                     /locus_tag="VW854_000263"
     CDS             12125..12802
                     /locus_tag="VW854_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0261564.1"
                     /translation="MHKILLVEDDQVIRQQVGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            12795..13769
                     /locus_tag="VW854_000264"
     CDS             12795..13769
                     /locus_tag="VW854_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEV0261565.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLLLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(13818..15761)
                     /gene="thrS"
                     /locus_tag="VW854_000265"
     CDS             complement(13818..15761)
                     /gene="thrS"
                     /locus_tag="VW854_000265"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEV0261566.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(15819..15995)
                     /locus_tag="VW854_000266"
     CDS             complement(15819..15995)
                     /locus_tag="VW854_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEV0261567.1"
                     /translation="MTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMENF
                     LSDKEKQLNVRTRK"
     gene            complement(16002..16142)
                     /locus_tag="VW854_000267"
     CDS             complement(16002..16142)
                     /locus_tag="VW854_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEV0261568.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDFKWTVEEDLLALSNDIGN
                     FQ"
     gene            17012..17641
                     /locus_tag="VW854_000268"
     CDS             17012..17641
                     /locus_tag="VW854_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV0261569.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTSDFPEEFKSLAKDQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKGISQISYADYAIGLVDELEKGHHIKEHISLLEK"
     gene            17782..18051
                     /gene="rpsO"
                     /locus_tag="VW854_000269"
     CDS             17782..18051
                     /gene="rpsO"
                     /locus_tag="VW854_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEV0261570.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            18866..19480
                     /locus_tag="VW854_000270"
     CDS             18866..19480
                     /locus_tag="VW854_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEV0261571.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(19772..20521)
                     /locus_tag="VW854_000271"
     CDS             complement(19772..20521)
                     /locus_tag="VW854_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEV0261572.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            20654..22990
                     /locus_tag="VW854_000272"
     CDS             20654..22990
                     /locus_tag="VW854_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEV0261573.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNLAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRRSMLRALPSALMVVFSVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTEQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            23092..23769
                     /locus_tag="VW854_000273"
     CDS             23092..23769
                     /locus_tag="VW854_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEV0261574.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAERILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            23756..24553
                     /locus_tag="VW854_000274"
     CDS             23756..24553
                     /locus_tag="VW854_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEV0261575.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSCGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQEDDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIATLLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            24570..25673
                     /locus_tag="VW854_000275"
     CDS             24570..25673
                     /locus_tag="VW854_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV0261576.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVAVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQEIDFYQRSGVFLLKKDESNLE
                     ELYQLALQRREESPLIGQLAILNQASANKLFPGLQGFDRLLYASGGARVDGQLLVTRL
                     LEVSHVKLVKEKVTLTPLASGYQIGEEEFEQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEDYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDEPLLQQME
                     EAALPHYPVLARVTSRAERVGIRAYTSDFSPFFGQVPELAGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPENYPIENYVKRVKSE"
     gene            25974..26993
                     /gene="galE"
                     /locus_tag="VW854_000276"
     CDS             25974..26993
                     /gene="galE"
                     /locus_tag="VW854_000276"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV0261577.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTDTLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            26995..27960
                     /locus_tag="VW854_000277"
     CDS             26995..27960
                     /locus_tag="VW854_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV0261578.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTHYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIVNFSDFPLTIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKNKVKRL"
     gene            complement(28011..28217)
                     /locus_tag="VW854_000278"
     CDS             complement(28011..28217)
                     /locus_tag="VW854_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEV0261579.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            28269..28745
                     /locus_tag="VW854_000279"
     CDS             28269..28745
                     /locus_tag="VW854_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEV0261580.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            28754..29425
                     /locus_tag="VW854_000280"
     CDS             28754..29425
                     /locus_tag="VW854_000280"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEV0261581.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            29556..29894
                     /locus_tag="VW854_000281"
     CDS             29556..29894
                     /locus_tag="VW854_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEV0261582.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            29897..30526
                     /locus_tag="VW854_000282"
     CDS             29897..30526
                     /locus_tag="VW854_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEV0261583.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITRHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWNPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            30767..31918
                     /locus_tag="VW854_000283"
     CDS             30767..31918
                     /locus_tag="VW854_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV0261584.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            32230..32979
                     /gene="truA"
                     /locus_tag="VW854_000284"
     CDS             32230..32979
                     /gene="truA"
                     /locus_tag="VW854_000284"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEV0261585.1"
                     /translation="MTRYKATISYDGYAFAGFQRQPHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVAADFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            32969..33742
                     /locus_tag="VW854_000285"
     CDS             32969..33742
                     /locus_tag="VW854_000285"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV0261586.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            33720..34181
                     /locus_tag="VW854_000286"
     CDS             33720..34181
                     /locus_tag="VW854_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV0261587.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
     gene            34408..35745
                     /locus_tag="VW854_000287"
                     /pseudo
     CDS             34408..35745
                     /locus_tag="VW854_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(35873..36076)
                     /locus_tag="VW854_000288"
     CDS             complement(35873..36076)
                     /locus_tag="VW854_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0261588.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(36069..37151)
                     /locus_tag="VW854_000289"
     CDS             complement(36069..37151)
                     /locus_tag="VW854_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV0261589.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(37277..38059)
                     /locus_tag="VW854_000290"
     CDS             complement(37277..38059)
                     /locus_tag="VW854_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEV0261590.1"
                     /translation="MVYTSLSSKDGNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(38059..39402)
                     /locus_tag="VW854_000291"
     CDS             complement(38059..39402)
                     /locus_tag="VW854_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEV0261591.1"
                     /translation="MNLKTTLGLLAGRSSHFILSRLGRGSTLPGKVALQFDKDILQNL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILEAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(39501..40817)
                     /locus_tag="VW854_000292"
     CDS             complement(39501..40817)
                     /locus_tag="VW854_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEV0261592.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQSLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            41010..42236
                     /locus_tag="VW854_000293"
     CDS             41010..42236
                     /locus_tag="VW854_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEV0261593.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPKVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GIVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            42435..44009
                     /locus_tag="VW854_000294"
     CDS             42435..44009
                     /locus_tag="VW854_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV0261594.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LNEKQTGFVIRNKGDK"
     gene            44274..45062
                     /gene="codY"
                     /locus_tag="VW854_000295"
     CDS             44274..45062
                     /gene="codY"
                     /locus_tag="VW854_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEV0261595.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            45062..45637
                     /locus_tag="VW854_000296"
     CDS             45062..45637
                     /locus_tag="VW854_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV0261596.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            45718..45803
                     /locus_tag="VW854_000297"
     tRNA            45718..45803
                     /locus_tag="VW854_000297"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45752..45754,aa:Leu,seq:aag)
     gene            46086..47216
                     /gene="ugpC"
                     /locus_tag="VW854_000298"
     CDS             46086..47216
                     /gene="ugpC"
                     /locus_tag="VW854_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEV0261597.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(47363..47509)
                     /locus_tag="VW854_000299"
     CDS             complement(47363..47509)
                     /locus_tag="VW854_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261598.1"
                     /translation="MEIKEQTRKLAVSCSKYIFEVADKTDEISNHTYGKATLTWFEEI
                     FEEY"
     gene            47717..48475
                     /locus_tag="VW854_000300"
     CDS             47717..48475
                     /locus_tag="VW854_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV0261599.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            48563..49075
                     /locus_tag="VW854_000301"
     CDS             48563..49075
                     /locus_tag="VW854_000301"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEV0261600.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKTTIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            49257..50201
                     /locus_tag="VW854_000302"
     CDS             49257..50201
                     /locus_tag="VW854_000302"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEV0261601.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYRYL"
     gene            50210..50887
                     /locus_tag="VW854_000303"
     CDS             50210..50887
                     /locus_tag="VW854_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEV0261602.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEISPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFIN
                     AMDLWKD"
     gene            50985..51743
                     /locus_tag="VW854_000304"
     CDS             50985..51743
                     /locus_tag="VW854_000304"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEV0261603.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            51781..53285
                     /gene="lytC"
                     /locus_tag="VW854_000305"
                     /pseudo
     CDS             51781..53285
                     /gene="lytC"
                     /locus_tag="VW854_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
     gene            53781..54299
                     /locus_tag="VW854_000306"
     CDS             53781..54299
                     /locus_tag="VW854_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEV0261604.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            54429..54935
                     /locus_tag="VW854_000307"
     CDS             54429..54935
                     /locus_tag="VW854_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEV0261605.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQDQEKNLYIIGG
                     KQIFQAFEPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            54935..55105
                     /locus_tag="VW854_000308"
     CDS             54935..55105
                     /locus_tag="VW854_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261606.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            55137..56369
                     /gene="clpX"
                     /locus_tag="VW854_000309"
     CDS             55137..56369
                     /gene="clpX"
                     /locus_tag="VW854_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEV0261607.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            56378..56965
                     /locus_tag="VW854_000310"
     CDS             56378..56965
                     /locus_tag="VW854_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEV0261608.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            56976..57356
                     /locus_tag="VW854_000311"
     CDS             56976..57356
                     /locus_tag="VW854_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEV0261609.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            57408..58298
                     /gene="rapZ"
                     /locus_tag="VW854_000312"
     CDS             57408..58298
                     /gene="rapZ"
                     /locus_tag="VW854_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEV0261610.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            58295..59272
                     /locus_tag="VW854_000313"
     CDS             58295..59272
                     /locus_tag="VW854_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEV0261611.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIHPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            59269..60180
                     /gene="whiA"
                     /locus_tag="VW854_000314"
     CDS             59269..60180
                     /gene="whiA"
                     /locus_tag="VW854_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEV0261612.1"
                     /translation="MSFTVAVKEEILGQHHLSWHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(60214..61179)
                     /locus_tag="VW854_000315"
     CDS             complement(60214..61179)
                     /locus_tag="VW854_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV0261613.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEFAITTSK
                     GSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSAV
                     DWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKLE
                     ITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYAI
                     GDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            61315..62172
                     /locus_tag="VW854_000316"
     CDS             61315..62172
                     /locus_tag="VW854_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEV0261614.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYTSTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            62159..62938
                     /locus_tag="VW854_000317"
     CDS             62159..62938
                     /locus_tag="VW854_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEV0261615.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            62962..64314
                     /locus_tag="VW854_000318"
     CDS             62962..64314
                     /locus_tag="VW854_000318"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEV0261616.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLT
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            64388..64762
                     /locus_tag="VW854_000319"
     CDS             64388..64762
                     /locus_tag="VW854_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEV0261617.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            64764..65612
                     /locus_tag="VW854_000320"
     CDS             64764..65612
                     /locus_tag="VW854_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV0261618.1"
                     /translation="MKLAVFTDSSAYLSAEILQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVENVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            65889..66656
                     /locus_tag="VW854_000321"
     CDS             65889..66656
                     /locus_tag="VW854_000321"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEV0261619.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVFDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            66668..67852
                     /locus_tag="VW854_000322"
     CDS             66668..67852
                     /locus_tag="VW854_000322"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEV0261620.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQNKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQARGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGDILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            67849..69720
                     /locus_tag="VW854_000323"
     CDS             67849..69720
                     /locus_tag="VW854_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV0261621.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELERAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKREVSGSSAETDLTMNFETSRIGKKVIEFQDVSFAYENKPILQD
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTAGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKAEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(69863..71047)
                     /gene="mntE"
                     /locus_tag="VW854_000324"
     CDS             complement(69863..71047)
                     /gene="mntE"
                     /locus_tag="VW854_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEV0261622.1"
                     /translation="MKQSISNLKLAERGAIISISTYLLLSAAKLATGHLLHSSSLVAD
                     GFNNISDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFLVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEDRF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKETYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
BASE COUNT        20831 a        13272 c        16258 g        20783 t
ORIGIN      
        1 tgcgttttat catggaaagg ttttttataa tagctaaaaa ataatagtta aatggttatt
       61 aattgcattc cctagtgatt tttgttaaga taaatgcaaa tacaaatgaa agcgagaaca
      121 agatgacacg ttatcaagat gatttttatg atgctatcaa tggagaatgg caacagacag
      181 ctgaaatccc agcagataag tctcaaacag gaggttttgt tgatttagac caggaaattg
      241 aagacctgat gctggcgaca acagacaagt ggttagcagg tgaagaagtg cctgaggatg
      301 ctatcttgga aaactttgtc aaataccacc gcctagttcg tgattttgac aagagagaag
      361 ctgacggtat cacacctgtc ttaccactcc ttaaagaatt ccaagaattg gagacttttg
      421 cggattttac agctaaacta gcagagtttg agcttgcagg aaaaccaaac ttccttcctt
      481 ttggtgtatc gccagacttt atggatgcta gaatcaatgt tctatgggct agcgctccaa
      541 gcacaatctt gccagatacg acctactatg cagaagaaca tcctcagcgc gaagaactct
      601 tgactctttg gaaagaaagc agcgcaaatc tcctcaaggc ttatgatttc tctgatgaag
      661 aaattgaaga cttgctagaa aaaagacttg aattggaccg ccgagttgcg gcagtggtgc
      721 tctctaatga agaaagttca gaatatgcta aactctatca tccatattct tacgaagatt
      781 tcaagaaatt cgcgcctgcc ctacctttgg atgacttctt caaagcagtt attgggcaat
      841 taccagacaa ggttattgta gacgaggaac gtttctggca agcagcagag caattctaca
      901 gtgaggaagc ctggtctctc cttaaagcaa ccttgatttt gagtgttgtc aatctttcaa
      961 ccagctattt aacagaggat atccgtgttt tgtctggtgc ctacagccgt gccctttctg
     1021 gagttccaga ggcaaaagat aaggtcaaag cagcttatca tctagcacag gaacctttca
     1081 agcaagccct ggggctttgg tacgcccgtg agaagttctc tccagaagcc aaggcggatg
     1141 tggagaaaaa agtggcaacc atgattgatg tctataagga gcgtctgctt aagaatgact
     1201 ggctcactcc agaaacctgt aaacaggcta tcgtgaagct caatgtgatc aaaccttata
     1261 ttggctatcc agaagaattg cctgcacgtt acaaggataa ggtagtgaat gaaactgcca
     1321 gtctttttga gaatgctcta gcctttgcgc gtgtggaaat caagcacagt tggagtaagt
     1381 ggaaccagcc tgtagactat aaggaatggg gcatgcctgc tcatatggtc aatgcctact
     1441 acaatcctca gaagaacctg attgtctttc cagcggccat tttacaggcg cctttctatg
     1501 acttgcatca gtcatcttct gctaactacg gtggtattgg ggcagtgatt gcccatgaaa
     1561 tttcccacgc ctttgatact aacggggctt cctttgacga aaatggtagc ctcaaggatt
     1621 ggtggacaga gagcgactat gctgccttca aggagaaaac acaaaaagtc attgaccaat
     1681 ttgatggaca ggattcttat ggagcaacca ttaacggtaa attgactgta tcagaaaacg
     1741 tggctgactt gggaggaatc gcagcagcgc ttgaagcagc taagagagaa gcagacttct
     1801 cagcagaaga gttcttctac aacttcggtc gcatctggcg catgaaaggt cgaccagaat
     1861 ttatgaaact tttggctagc gtcgatgtgc acgcaccagc caaactccgt gtcaatgtgc
     1921 aagtaccaaa cttcgacgat ttctttacaa cctatgatgt caaagaagga gacggaatgt
     1981 ggcgttcacc agaggagcgc gtgattattt ggtaatacaa aaaatctagt catcagaaaa
     2041 aaggcatcca atcacgtgtg aaaaccttga taggatgcct tttgtaatag aaagatttgc
     2101 tggatagttt acttaaattt cgattgtcat catcttgcta gaaaaaggga ttgaaggcct
     2161 tttcgtgagc gatagtagta gctggaccat gtcctggata gacatcgtag tttggtaggg
     2221 tgaagagttg ggtctggata ctatgaagga gttgctccat gctaccagtc ggaaggtcgg
     2281 tccgtccgat agtttcgcgg aatagagcat ctcccgtcaa gactaggtga gcatcaggaa
     2341 agactaggga aacaccaccg atagagtgcc ctggggtcgg tagaacctta aaacgaaatt
     2401 cctctagttg gtattcttcg tgaaagacaa aggtgtgttc tgcaggtttt gtgaccacat
     2461 ctgccatatc atcgtggcga gggagaccgg agagattatc gacaggagtg tagagccagc
     2521 tggcttcgct ctctgcgata tagacaggag gattgccaaa cgtctcgcga accaagtcca
     2581 gactcatgat atggtcataa tgggcgtggg tcaagagaat agcacagatc ggtttgttga
     2641 tcttctcgat tgtctgacga atggcttccc aatgactacc aggatcgacg acgatgaggt
     2701 gcttttcgcc ttctagataa taggtatttt cataggcaac aggattcacg gttttatgga
     2761 ttttcatact agctccaatc tcaaagaatg tactaatact cttcgaaaat ctcttcaaac
     2821 cgcgtcaact ttatctgcaa cctcaaagca gtgctttgaa cagcctgcgg ctagctttct
     2881 agtttgctct ttgattttca ttgagtataa attaagtata gcacagttag ggagaatagg
     2941 taaggattta aaatgaactt aaaaaccagc atgactagat gaagacaggc tggttatcaa
     3001 tttatcaata ttggaaaggt gtcaattgcc cttcataggt tgcataaact ccgtcattag
     3061 cttgtttgat agacgtgatg tttagagtgc taccgtagtt ggcttctcat ttgtcgctat
     3121 attcactata agttatccta ctgggcaggt tctggtcgtt agcatagtat tgaacgagtt
     3181 ttttatagct ttgctggctg aataggaaca ggcagcaaac tcctaacatt aggagagcaa
     3241 agatagaaat agcagttttt tcataggtat tctccttata aactaaagga tagaagaaag
     3301 tatcttgagg atttttatac tcttcaaaaa tctcttcaaa ccgcatcaac gtcgccttgc
     3361 cgtatatatg tgactgactt tgtcagtctt atctacaacc tcaaagcagt gctttgaaca
     3421 gcctgcggct agtttcctag tttgctcttg gattttcatt gagtattaat tccaaaatcc
     3481 agtcaaggta ccagtgtatg tgatacgagt tccaccgttt atctttcgta tcttcaatgg
     3541 aattggggaa gtcagaaagt ctttgggtta caatatggtt tccgttcttc tatctctcct
     3601 tctatcacct aagtttaaaa tctaacttcg tagtatatta tggaccaata attcagaatt
     3661 atcaagcaat tttgaattat ttttttctta ttctgttcca atctagtttt catattctta
     3721 aaaaagtgat aaaatggtag gaagaattgg agagtagaga tgccgaaaga agtgaattta
     3781 acaggcgaag aagttgtcgc tttaaccaaa gaatatttaa cggaagagga tgttcatttt
     3841 gtccataagg ccttggtcta tgctgttgaa tgccacagtg gtcaatatcg caaatcaggc
     3901 gagccttata tcattcaccc tatccaagtg gcaggtattt tagctaagct aaagctggat
     3961 gctgtaacag tagcttgtgg attcttgcat gatgtggtgg aagatacaga tgcgaccttg
     4021 gacgatttgg aaagagagtt tggtcctgat gtgcgggtga ttgttgacgg agttaccaag
     4081 cttggcaagg tcgagtacaa atcaatcgag gagcaattag cggaaaatca tcgcaagatg
     4141 ctcatggcca tgtctgagga catccgcgtt attttggtca aactgtctga ccgcttgcac
     4201 aatatgcgga ccctgaaaca tcttcgaaaa gacaagcagg agcgtatttc caaagaaacc
     4261 atggaaatct atgccccact tgcccatcgt ttggggattt ccagtgtcaa atgggaatta
     4321 gaagacttgt ctttccgtta tctcaatcca acggagtttt acaagattac ccatatgatg
     4381 aaggaaaagc gcagggagcg tgaggccttg gtggatgagg tagtcacaaa attagaggag
     4441 tatacgacag aacgtcactt gaaagggaag atttatggtc gtcccaagca tatttactca
     4501 attttccgca aaatgcagga caagagaaaa cggtttgagg aaatctatga tctgattgct
     4561 attcgttgta ttttagatac ccaaagtgat gtttatgcca tgcttggtta cgtgcatgaa
     4621 ttttggaaac cgatgccagg tcgcttcaaa gactatatcg ccaaccgcaa ggccaatggt
     4681 tatcagtcta tccatacgac tgtttatgga ccaaaagggc cgattgaatt ccagattcga
     4741 accaaggaaa tgcacgaggt ggctgagtac ggggttgcgg ctcactgggc ttataagaaa
     4801 ggtataaagg ggcaagttaa cagcaaggaa tcagctattg gaatgaactg gatcaaggag
     4861 atgatggagc tccaagacca ggctgatgat gctaaggaat ttgtggactc tgttaaggaa
     4921 aactatttgg ctgaggagat ttacgttttt accccagatg gagctgtccg ttcccttccc
     4981 aaagattcag gaccgattga ttttgcctac gaaatccata ccaaggtcgg tgaaaaagca
     5041 actggtgcca aggtcaatgg ccgcatggtt ccactgacaa ccaagttaaa gacaggggat
     5101 caggttgaaa ttatcgccaa cccgaactcc tttggaccta gccgtgactg gctcaatatg
     5161 gtcaagacta gcaaggcgcg caataagatt cgccagttct ttaaaaacca agataaggaa
     5221 ttgtctgtca acaagggtcg tgagatgctg atggctcagt tccaagaaaa tggctatgtg
     5281 gcaaataaat ttatggacaa gcgccacatg gatcaagttc tgcaaaagac cagttacaag
     5341 acagaagact ccctctttgc ggccattggt tttggggaaa tcggtgcgat taccgtcttt
     5401 aaccgtctga ctgaaaagga acgccgtgag gaagagcgtg ccaaggccaa ggctgaggca
     5461 gaggagcttg tcaaaggtgg cgaggtcaag gttgaaaata aagaaactct caaggtcaag
     5521 catgaggggg gagtggttat tgaaggtgct tctggtctcc tagtgcggat tgctaagtgt
     5581 tgtaaccccg tgcctggtga cgatattgtt ggctacatta ccaagggtcg tggtgtggct
     5641 attcaccgtg tggactgtat gaacctgcgt gcccaagaaa actacgagca acgtctcctt
     5701 gatgtggaat gggaagacca gtactctagc tcaaataagg agtatatggc ccatatcgat
     5761 atctacggtc tcaaccgtac aggactgttg aacgatgtac tgcaagttct ttcaaataca
     5821 accaagaata tttcaacggt caatgcccaa ccaaccaagg atatgaagtt tgctaatatc
     5881 catgtgtcct tcggtattgc caacctctct acactgacta cggttgtcga taaaattaag
     5941 agtgtgccag aagtttactc tgtcaaacgg accaacggct agttgtccta gctcttacta
     6001 gaaaggctat tatgaaaatc attatccaac gggttaaaaa agcccaagtg agtatagaag
     6061 gccagattca gggaaaaatc aatcagggac ttttattgct agttggtgtt ggaccagagg
     6121 accaagagga agatttggac tatgctgtga gaaaactggt caatatgcgg attttttcag
     6181 acgcagaagg caagatgaac ctgtctgtca aagatattga aggagaaatc ctctctattt
     6241 ctcagtttac cctctttgcg gatactaaga aaggcaatcg tccagccttt acaggggcag
     6301 ctaaacctga tatggcatca gacttctatg atgctttcaa tcaaaaatta gcgcaagaag
     6361 tgcccgttca gacaggtatc tttggagcag atatgcaggt tgagctggtt aataacggac
     6421 ctgttaccat tatcctagat actaaaaaga gataagaaag accaagccca gtcggcttgg
     6481 tctttctcat cgatcataaa atactccaaa aagaaatcgg ttcttgatat gcttggggga
     6541 agtctctttt caggctttgg cagatgcgat aggaagggat gagatgtcct aggatgagga
     6601 gagttccctg aaggaaaagt ggaatatcac ttaaagacac caaggagaga atcatcaggc
     6661 tatcccatag gagaatttgt aaagcaaaac gccagaatct cggcctaatc tgggaataga
     6721 gttgactaaa gtggtcacaa agttggtttg aaagataggt caatagcaca ggatgaaaga
     6781 aaatgagcca accactataa atcaaaatcc agtcccaagt aagcccccct tttaaatgtc
     6841 aaaaatgcca gcatgattcc atcaatgaca aggagttgaa tggttttgat gaagatgatt
     6901 tttttcatgc taaaacctcc ttttccttag agacactccc ataaagagat gtgtttgtcg
     6961 taaaagtctg gatagttttc tcttaggtag tcagctgtca tataataaaa agtagaatgg
     7021 caagcagtga taattgacat aagagatgaa aaacgctgag aactaatgaa ggctaagaga
     7081 acaaaaaata gaaaatcaat ggtgagaacc cctaaatagt agaagcgaat ctttttattg
     7141 atttgaggat aaatctcagc gaattgatgt ttgagtcgga cgatcaagag aaatagcagg
     7201 agtccttgag caaggaagaa aattaaacca gagagcaaga gccactcaaa agatgtctcg
     7261 agtctagtga taaaaaatgc aaatagtagc aaatcaataa ctgcaattgc tataaaatgg
     7321 attctataga gttttttcat gacaagacct tcctctttta tctaacttca ttctactcca
     7381 aaagaatggg agttacaact aaaatgataa aaatagcaga agggagattc tcttaagttg
     7441 gctagtattc tttatttgag tttccttcta tgtatctaac ttcttcatca ttccagacaa
     7501 ataaagctcc gattgcattg aggatataaa agatgtattt accgatattg gcgaagtttc
     7561 cttgaatacc agcttttgtc agctgaacga aattgtaaat caaccaaaag ccccactgag
     7621 ttgttagttt caatgcattc aaagcattgg caatgaggga cagtgcaaag gcaatagttg
     7681 ttacgtaggc aaggagatcc atcttgccct catatccgat atagttggtc acaaaggcaa
     7741 agaggaaggc gatgatggaa atgatgatgg ccgccaattt tacctgtttt tggctcattt
     7801 ggttgggtct gccttcttgc gaagcttccc acttctttat agcaaaggta taaatgagga
     7861 aggtgacggg ataggtaatg atggccgcct tatttccaag gatataatca atagcaccga
     7921 acaaaagggt attaacaata ccaaagtaat ttccccattt gcttaatttc cccgtgaaac
     7981 gagtggacaa catggaaatc ccaacgttgg ttacggaaat caatccaaag ggtacaagag
     8041 ctgtccatga tccccagtct acaaatttat cgaggcgtga gttgagataa ccagatgcaa
     8101 tcgcaatccc aacgaccaaa gcaaccccga agaggtcaaa tcatttagat gtagcaaaaa
     8161 tttttagtga ttttttcata ggttaaacta cctttctttt tttcaaatat tctcccattt
     8221 tttttcaaat attctaccac caaatgaaag taaaataaaa tgatagaaat aaaaccctga
     8281 aaataaaact ttcagggttg gtaggggact tgagaaatta gttgattttc tcgacataga
     8341 gttgttttgc aatgtccata ttcacttgta aatcctcgtc gttactgagg atagtgaagg
     8401 tattgctgaa gccatcaaac tgcttgactt ggagctggtc accgatgtga agtgagtgct
     8461 tgtccagata atggagaatg tcaaaattat cgtgcacccg agtcaggcgg taggcgccag
     8521 cttccttgat atcagctagt gggaggttat tgatttcaac gaggagttct cccttggcag
     8581 gaatagttcc tccgtggggg caggttttag ggaaacctag cagtttatct agtctttcca
     8641 cgaacaggtc agagacagtg tgttccaaga cctcagcttc ctcgtgaatc tggtcacttg
     8701 tatagtctaa atgatgaact agaaaaactt caatcaagcg gtgcttacga tagagctcag
     8761 agactagttt gagaccgagg tcagtcagta gatagccaca ttccttgtcc tttaggatga
     8821 ggttttcact tttcattcgt ttgatcattt cagttacggc agggggagag acttgcatgc
     8881 gagccgcaat ttccttgttg gtaatcttat gcaggtctat gccaatttca taaatacatt
     8941 ttagatagtc ttctttgttt ggggtcattc gtttcctctt gtctaatatc tttatctagt
     9001 atatcaaaaa aagctagatt tctctagctg tgacttttta tgttatactt attgcaagag
     9061 aaaaaacgag gagatggata tgacgaaaat agctcttctt tcagatattc atggaaatac
     9121 caccgccttg gaggctgttt tggcagatgc tcggcagata ggagtggatg aatactggct
     9181 tttgggagat attctcatgc cagggacagg acgtagaagg attttggact tgttggatca
     9241 actaccgatt acggctagag ttttgggaaa ctgggaagac agtctttggc atggtgtccg
     9301 taaggaattg gacagtactc gccccagtca acgctatctc ttgcgccagt gccagtatgt
     9361 tttagaggaa atttccctag aagaaattga agtgctccac aatcaacctc ttcaaattca
     9421 tcgtcagttt ggggatttga cggtgggaat tagccatcat ctgcctgata agaactgggg
     9481 gcgagagttg attcatactg gcaaacaaga ggagtttgac cgcttggtga ctcatcctcc
     9541 ctgtgatatt gctgtttatg gacatattca ccagcagttg cttcgttacg ggactggtgg
     9601 gcaattgatt gtcaatccgg gttcgattgg ccaacctttc tttttggacg ctcagctacg
     9661 gaaggacttg cgggcccagt atatgatttt ggagtttgat gacaaaggcc tggtagatat
     9721 ggacttccga cgggtagact acgatgtggc agcggaattg cagctggcta aagacctgag
     9781 acttccctat tttgaggttt actatgaaag tctggtcaat gggatccacc atactcatca
     9841 tcaggaattt ttgagagaat tggctcagaa agagggctgc gatcgggagt tagacgactg
     9901 gttgaaaagt ggtaacgatt gacattacaa ctaaaaaggg tataataata gaaaaagaag
     9961 agtagaggca gggtagcctc gtaaaaagga gaagaggatg caaattccaa gtagatttac
    10021 cattgcgact catatgctga taatcattgc cctcgagggg aaggaaagca aggtgaccag
    10081 tgattttctg gctgctagtg tcggggtcaa tcctgtcatt atcagaaaga tcttgtccca
    10141 gttgaagaag gcagagctga tttcagtagc gcgtggaacg ggcggaacag agattgtcaa
    10201 ggaccttaag gatattagtc ttttagatgt ttatcaggcg gtcgaatgtc ttggtaagac
    10261 aggtcaactc ttcagttttc atgacaatcc gaatccaaat tgccctgtag gagctcatat
    10321 tcatgatgtt ttggatcaaa aattggagag aattcagttg actatggagg cagaacttgg
    10381 tcaaaccagt ctagaaaaag tcgtggccga tgcagagagt cagatgaagg attaagaggg
    10441 ataaatgccc tcttttttct ttggataatt atgataaaat gtagctatta aaggagtaag
    10501 aaatgtatct cgttatagga attgtattag cctttatagt gtcattttgg aaggataata
    10561 gaagtttgtg gaatccagta ttatttttat tatccctcat ttcaagttat ttttatctat
    10621 cctacctttt ttataaaaat ggatacgaga atgttcagtt agctttttat gtatttgcct
    10681 ttgtcttact tccttttttg ctttttctaa gtggcatctt tttaatctat aatggggtca
    10741 ttttgttgaa gcgagaggga cgttccaagc cccattatct ctcaatgctc tttgattttt
    10801 attgactatt agattctatt taaaaagatt tagaatgcac atgaaagtat gtattctagg
    10861 tcttttgttt tgtatacatt taattttcaa gagtattatc tatttttcca tgcctggtaa
    10921 cgagtatcaa tcaataactg ggtaaggcga cccaaggttt ctctttcttc tggagtgagg
    10981 gattgagctt ggacaaagag atgatgaatg tattcaagcg cctttaagga agccaagtca
    11041 ttgatagagc taataccagc ctcaagaata gtgccaaaga agaggtcgaa gtagagctga
    11101 agttcttcgt cagggactgt ggccacccat tctttaaagg ttgtgtctac ttgctggcta
    11161 tcactgttgg tcttatccag ttggacgaag tgcttgtcct caatctgcca actaaaggta
    11221 tcgtgctggg cgatgccacc cagggcagta ctctgaacga tgatttggtg agcaggaatt
    11281 tccagcatca taccgataat ggaaccttgt ggaatgaata tcttgcttct atccattatc
    11341 ctttgataac ccgcagtctg tgtcaattct tgatggagac caggtgcatc aaatgtataa
    11401 actgctgtga tctgattttg caaactttgc tcaatttggc tagcagcata gatagcgaga
    11461 tttcctccct tggaatgccc agccagaata actttttgct taggatgatg ggcaaaaaag
    11521 ttctttaaat agcgaagggc gtgcttttga gcaggaattt ccttcatata ggtcaggtgg
    11581 aaatcttcct tccagccaat gatactgtca tctgtcccac gaaagacaat cagataggta
    11641 tcgaggctga cacgataagt catagccgca aattgctttt gcagttcagg gtcgatgtcg
    11701 ttgataaaat gggagagttt gcaatttttg aagcgcttgt gttgagccaa tttatctaat
    11761 aattgaaggc gatttttgct agtaagcatg gtgggctctc ttggaacctg aggtgctaga
    11821 tctaaaagac gttgaggaag tgtggagacc agattatcaa aggagaggta ggtgatttct
    11881 attaaggtta gaatgtctaa ctcattcaag ggaaggtcgt aataagaatc atatgcgaca
    11941 tctttcagat agtcaaaaat attggccata agagctcctt tctttttatt tatcttagca
    12001 aatttccctc aaattagcta gtagcattgc ctgtttgtac tggctaaaaa caggctattt
    12061 caaattcagt ttcagaccat ctggcatgga aaaatctgtt ataataatgg aaaaggagaa
    12121 gcgcatgcac aagattttat tagtggaaga tgatcaggtc attcgtcaac aggttgggaa
    12181 aatgctctct gaatggggat ttgaagtggt cctggtagaa gactttatgg aagttttgag
    12241 tctatttgtt cagtcggaac ctcatctggt cctcatggat attggtttgc ccttgtttaa
    12301 tggttatcac tggtgtcagg aaatccgcaa gatttccaag gtacctatca tgtttctttc
    12361 ttcgagagac caggctatgg atattgtcat ggcaatcaat atgggggcgg atgactttgt
    12421 gaccaagcct tttgaccagc aggttctttt agctaaggtt cagggcttgt tgcgtcgttc
    12481 ctatgagttt gggcgtgatg agagtttgct ggaatatgct ggtgttatcc tcaataccaa
    12541 atccatggat ttacattatc aagggcaagt cttgaatttg accaagaacg aattccagat
    12601 tttacgcgtg ttatttgagc atgcaggcaa catcgtagca cgtgacgacc tgatgcggga
    12661 actttggaac agtgactttt tcattgatga taataccctc tctgtcaatg tggctcgttt
    12721 gcgtaaaaag ttggaggagc agggattggt aggatttatc gagaccaaga aaggaatagg
    12781 gtacggattg aagcatgctt gattggaaac aattttttct agcctatcta cgctcccgta
    12841 gtcgtctttt tatctatctg ctttctttgg catttcttgt cttactcttt cagtttttat
    12901 ttgccagtct aggaatttac ttcctctact ttttcttctt gtgttgcttt gtaaccatct
    12961 tatttttcac ttgggacata ttggtggaaa cgcaggtcta tcgccaggaa cttctctatg
    13021 gagagaggga agccaagtct cctttggaaa tagctttagc agaaaaatta gaagcgcgtg
    13081 agatggaact ctatcagcag aggtcaaaag cagaaagaaa actgacggat ttgctggatt
    13141 actatacctt gtgggtccat cagataaaga cccccattgc agccagtcaa ctcttagttg
    13201 cagaagtggt cgaccgccaa ctgaagcagc agctagaaca ggaaattttc aaaatcgact
    13261 cctataccaa cctagtttta cagtacctgc gtttagaaag tttccatgat gatttgctct
    13321 taaagcaggt tcaaattgag gacttggtca aggaaataat tcgtaaatat gctcttttct
    13381 ttattcaaaa aggcttaaat gtcaatctac atgaccttga taaagaaatc gtgacggata
    13441 aaaagtggct gctagtggtt attgagcaaa tcatctcaaa cagtctcaag tacaccaagg
    13501 aaggtggtct ggagatttat atggatgacc aagagctttg tatcaaagat acgggaatcg
    13561 ggataaaaaa cagtgatgtc ctccgagtat ttgaacgtgg cttttcagga tacaatggcc
    13621 gtttgaccca gcagtcctct ggacttggcc tttatctatc taagaaaatt tctgaagaac
    13681 tggggcacca gattcgtatc gagtctgagg tcggaaaagg aacgacagtg cggattcagt
    13741 ttgctcaagt gaacttagtc cttgagtaaa aatagatgag aaaaagcctc cagattggag
    13801 gcttttatgc tagactctta tttctcaacg cgtgatttgt tggcgatatc agctaggata
    13861 gcttgaacaa aattatcaac tgagacagtt tgtgtttctt tttggccgta gcgacgaacg
    13921 ttgactgttt cgtcttccat ttctttgtct ccaacaatta attggtaagg aatcttgctg
    13981 gtttgtgaag cacggatctt gaactgcatt ttttcattgc gctcatctac gtctgcacgg
    14041 acaccgcggt cacggagttt cttggccact tcccaagcgt agtccacgtg tttttcgtta
    14101 gatactggga tgagggttac ttggtgtggt gccagccatg ttgggaaggc ccccttgtag
    14161 ttctcaatca agatagctgt gaagcgttcc atagttgaga taaccccacg gtggatcatg
    14221 actggacggt gatcttcgcc atcagctccg atgtatttga ggtcgaagcg ttctggcaac
    14281 aagaaatcaa gttggatagt agaaagggtt tcttcttttc caagggcagt tttaatctgg
    14341 atatccaatt ttggtccgta gaaggctgct tcaccttcgg cttcaaagta gtccacgccc
    14401 atttcatcaa gagctgcacg aagcatggtt tgggcatttt cccacatctc atcgttatca
    14461 aagtacttat gagtatcttg agggtcacga agagagaggc ggaagcggta gtcagtcaag
    14521 ttgaagtctt cataaacatc gataatcaac tgaagggcac gttggaattc ttcttggatt
    14581 tgttctggag taacgaatag gtgaccgtcg ttgagtgaca tttcacgtac acgttgaagg
    14641 ccagtgaggg caccagattt ttcgtaacgg tgcatcatac cgatttcagc gatacggatt
    14701 ggcaattcac ggtaagagtg aacatggtgt ttgaaaactt ggatgtggtg cggacagttc
    14761 attggacgaa ggacaaattc ttccccgtca cccatgtcca tggttgggaa catgtcttct
    14821 tggtaatgat cccagtgacc agaagtcttg taaagctcaa cagaagcaag tggtggagtg
    14881 tagacgtgtt ggtagccaga agccaactct ttgtttacga tgtagcgttc caattcacga
    14941 cggatagtcg caccatttgg taaccagaat ggcaaacctt gtcccacttc ttgtgaaatc
    15001 ataaagaggt caagctcttt accaagttta cggtggtcac gttccttagc ttcttcacgc
    15061 atttgaaggt agtttttcaa gtctttcttg tcaaaccaag ctgtaccata gatacgttgc
    15121 atcatagcgt tgtcgctgtt tccacgccag tacgcaccag ctacatggag aaggtggaag
    15181 atttggatac gacctgttga tggaacatga ggtccacggc agaggtctac atattcaccc
    15241 tgacgataga tagtcaaacc gccttcgtct tctgagtgtt cttcaatcaa ttccaacttg
    15301 taagggtcat ttttgaagat ttcacgtgcc tcgtctttag tcacttcttc acgaatagat
    15361 gggaagtttt ctttgacgat tttttgcatt tcttcttcga tacgaggaag gtcttcgtta
    15421 gagatttgac cagctgtgtt gtcagtatcg tagtagaaac catcttcgat ggctggacca
    15481 actcccaagt gaatgtctgg gaaaagacga cgagctgctt gggcgaacaa gtgagctgct
    15541 gagtgacgca agattggaag ggcatcttcg tgatcaggtg tcacaatttc gatgcttcca
    15601 tcttcagtga tagcgcgagt agtgtcgatg agtttgccgt tgaatttacc agccaaggct
    15661 tttttagcta gggaattgct gatagattgg gcaatttcaa aagttgttac gccagattcg
    15721 aattcacgaa cagcgccatc tgggaaagta atgttaatca tgatgttctc cttttttatt
    15781 atttcatagt ttctaagcat tgatttttta tcagcagact acttccgagt cctaacgttc
    15841 aattgctttt ctttatcaga gaggaagttt tccatttccg tcagaatgtc tatatccaaa
    15901 cgttgagaaa gttctaatag ccaccagata ttttctgaaa gtttttgttc cagtgtgtag
    15961 ggtgtttcat catagtagcg tccttgcttt gtcatcacca gtcattggaa atttccaata
    16021 tcattagata aagccaagag gtcttcttct accgtccact tgaaatcatg atgcttaact
    16081 tccagttcgt gataagcttg tcggattgcc caactgcgct ccactaattc ttgcaactcc
    16141 ataattagta tcctaataaa aaaatgcgac atcctcgaaa ggacgtcgca acgtggttcc
    16201 accttcattt atgtacctca aaagagaggg acacctctga tcggctctaa cgtggccacc
    16261 gttttatgtt ttcataaaaa actcaagagt agtatcagtt tagacttcct atgcgtttcc
    16321 agcaaccacg cactctctag tagaaaggga ctaagtgact tgtctctatg gattattata
    16381 gcatgattgt gagatttttc aaggattttg tgaaagtttt ttgtttttca ttttttgtag
    16441 aatatgcttg atgctaatac cagaatccat gccgaccaga gcaactgttt catagattag
    16501 caagtaaatt agtataaact caccaaaggt tatgatagta aagtaaaaga gcagtccgtt
    16561 accaactaac catcaaagtg agaaacggaa acggtagcta tagagtaacg atataatcaa
    16621 gattaccaga ggtgtaaaga agagaagatt gtttatatag agttccttga gtgtaagggg
    16681 gtcagaaaca actagtaaga acaactcata aagtggtcaa taaaagaata atatgaccag
    16741 atagtagggg agatgggaat agaatctacg catgataatc acctcgtttt aaaaaaacag
    16801 acaacttaga tctcttgcta tgattattat agcatgttta aaacaagaaa gtaaatttgt
    16861 tgacaaagaa gatttttctt ttcactagaa tcatagttag ttttcttggt agaaatattt
    16921 tgataataat taagcaagac tattgacatt gattattaag agtgtcacaa ttattgtaac
    16981 taaaataatt acaacaagag aggtataaaa aatgaaacta gcagttattg cagcaaatgg
    17041 acaagcgggg aaagcaattg ttgaagaagc agtcaaacga ggacatgaag taacggctat
    17101 tgttcgttct gaaaacaaaa gtcaagctga gtctattatt aaaaaagatt tatttgagtt
    17161 gacaaaagat gatcttactg gatttgatgc tgttatttca gcgtttggtg cttatacacc
    17221 agatacgcta ccgctccaca gcaaatcaat cgaactattt aatcagcttc ttgctggaac
    17281 acagacacgt tttctagttg tagggggagc aggaagtctt tatatagatg aaacaaaaac
    17341 gacacgactt ttagatacgt ctgattttcc tgaagaattt aagtcactag caaaagatca
    17401 agcggatgaa ttggatcttc ttcgtacaaa aaataatctc aactggactt ttgtcagtcc
    17461 tgctgtagat tttattcccg atggtgaaaa aacaggaaac tacattctag caggagaaat
    17521 ttttactact aatgaaaaag gaataagcca gattagttat gcagattatg ctattggatt
    17581 ggtagatgaa cttgaaaaag gtcatcatat aaaagaacat atctctttac ttgaaaaata
    17641 ataactatga gagtattaat gtttgacaga aaatttaaat cttggtaaaa tagggattgt
    17701 cgtaaagaca aataacttct tcttggttac aggcatgcca acctgtcact cggatgaagc
    17761 caaataaaaa ggagaaacat catggcaatc tcaaaagaga aaaaaaatga aatcattgca
    17821 caatatgcac gtcacgaagg tgatacaggt tcagtagagg ttcaagttgc tgtccttact
    17881 tgggaaatca accaccttaa cgaacacatc aaacaacaca aaaaagacca cgctacttac
    17941 cgtggattga tgaaaaaaat cggtcgccgt cgtaacttgc ttgcatactt gcgtaaaaac
    18001 gacgttaacc gttaccgtga gttgatcaac tctctaggac ttcgtcgcta attcaagata
    18061 caaatgctgt caaaagcaca aagcaaaaat aggaaaattg acgaagaaac ttcagtttct
    18121 aggagatttt atcttttttg ccaagtgctt aggccgtgtt caattgagca tatcttgata
    18181 atgaagctac tctaaattgg gtagcttttt tgcgttgtca ccttacctcg ctatactcaa
    18241 gtataatctt tggttacggt tcctagcact gtaaggtaaa ataaaccagc ttttctccct
    18301 tcggggagat tttttttgtt tcactgaaag tttcgtacga tcttcgcctc gtcgcctagt
    18361 ctataaacga tttatctagc aagaatcatg atgctaaggg cgtcaaaaat ccgtatgaaa
    18421 atagggaaat gacacagtgt tcgatgagca caaggagttt catctttttc actaggattt
    18481 tagcccgggc tcaaatcagc tctctgactt cagagggctt ttttattgag gttttatcgg
    18541 taacaatttt ggagactaca aaaacctcaa acggtatcag ctaattacac cataaaggtg
    18601 cgcagttact cggcttttca agccgagtag ctgtatgcaa gccccctaga agagcccact
    18661 ctttacaaag taaagtatag tatgttatac tttacatgga agtagtcact gaattccagt
    18721 tagaaattac tttgtaacta cgttttgagg aggagtaaaa tgctttccta cgttcgacat
    18781 tacccactag cgatagctaa attaatgtgt ctgtgctctc ctaaaatctg ctgatttatt
    18841 actgactaat acaggagttt tttttatggg acagacaatc atatctgcta ttggtgttta
    18901 tatttccacc agtatcgatt atttaattat tttaattatt ttatttgcac agctatcaca
    18961 gaataaacag aaatggcata tttatgcggg gcaatatcta ggcacaggct tacttgtagg
    19021 ggcgagttta gttgctgctt atgtcgttaa tttcgtgcct gaagaatgga tggttggatt
    19081 gcttggttta atccctatct atttagggat tcgctttgca attgttggag aagatgcgga
    19141 agaagaagag gaagaaatta ttgaaagatt agaacaaagc aaggcaaatc aactgttttg
    19201 gacagttaca ttgctgacaa ttgcgtctgg cggagataat ttaggtatct atatacctta
    19261 ttttgcttcg ttagattggt cacagaccct cgtggccttg cttgtgtttg taatcggcat
    19321 aattatcttt tgcgagatta gtcgggtgtt atcctctatt ccgttaatat tcgagacaat
    19381 tgaaaaatac gagcgaatca ttgtgccctt agtattcatt ctacttggac tatacatcat
    19441 gtatgaaaat ggcacgatag agacttttct gatcgtgtag atttttttgt ttcactagga
    19501 ttttagcccg agctcaaatc agctctctga ttttcagagg gctttttcat tgagtataaa
    19561 tggacaatct taaagttact ctattgtaga gtggcttttg atttgattag gagattctta
    19621 taaatatagt aaaatgaaat aagaatagga caaatcgatc aggacagtca aatcgatttc
    19681 taaaaatgtt ttagaagtag aggtgtacta ttctagttcc aatctactat ataaatgaaa
    19741 accttagctg tttggccaag gttcatttct tctatgcaag ttcttctctt ttcttatctg
    19801 ggttccagat gaagcttgcg ataaagctaa agatgatggt taggatagcg aggataatag
    19861 caaggatgag ggcagggatg cggataaacc accagagtgg atttggtttt ttatcattgt
    19921 gccattgttt cgtttcttcg tccagacgtt ggaattctgt ttgaatacga ttggcgactg
    19981 tttcaatgcc ttcatcattc attttcttga tatctgagat atcgattgga tttccaaagt
    20041 tcatatcgac acgttcacgg ctaatcaagc ccttcaaagt catgggaccg gtgtaggtaa
    20101 ccggcatgat acggaccttg gccattttgg caatcagtgc tgcgcccccc ttgacatcgt
    20161 ttgagtggcg gctaccactt ggaaacatga tgagagagcg gtcacttttt ttgagaacgt
    20221 tgataggata tttgatggct gaggcgctgg gattttcacg gtcgatggga aaggcgccac
    20281 acatacgaat ccaccaacca aagatacggt tggtaaagag ttcttttttt gccataaaga
    20341 tgaactgttt tggcttggtc gcaaaggcca tataaacagg atcccaccag gtacggtgag
    20401 gcgcaactaa aatataattt tcatcttgat taggaatttt atcagtatta tgatagtgag
    20461 cattgccatt gatggaccat aagagcaata caactaatcc acgcaaataa gtataaaaca
    20521 tgcgatctcc ttcgattgtt ttcttgttat tattatacct tatcaaagga gggctggcaa
    20581 acttttccct tgactagata catatttagg atgaaattag aattctgtta aaaaaaatga
    20641 tataatagaa tttatggata aaaataagat tatgggatta acccaaagag aagtcaagga
    20701 aagacaggct gagggtttgg tcaatgactt taccgcatca gccagtacca gcacttggca
    20761 aatcgttaaa cgaaatgtct ttaccctttt taacgctttg aacttagcca ttgctttggc
    20821 tcttgccttt gtgcaggctt ggagcaatct ggtcttcttt gctgttatct gctttaacgc
    20881 tttttctggg attgtgaccg agctacgagc caaacacatg gtggacaagc tcaatctcat
    20941 gaccaaggaa aaggtcaaaa ccatccgtga tggtcaggaa gttgctctta atcctgaaga
    21001 attagtgcta ggagatgtca ttcgtttgtc tgcaggagag cagattccta gtgatgcctt
    21061 ggttttggaa ggctttgcgg aagtcaatga agccatgtta acgggagaaa gtgatttggt
    21121 gcaaaaggaa gttgacggct tacttttgtc aggaagtttc ctagccagtg ggtcagtttt
    21181 atctcaagtt caccatgtcg gtgcagacaa ctatgctgcc aaactcatgc ttgaggctaa
    21241 gaccgttaaa cccatcaact cccgtatcat gaaatcgctg gacaagttgg ctggttttac
    21301 tgggaagatt atcattccct ttggtctggc tctcttgctg gaagccttgc ttttaaaagg
    21361 cctgcctctc aagtcatccg ttgtaaactc gtcgacagct cttttgggaa tgttgcctaa
    21421 gggaattgcc cttttgacca ttacttcgct cttgactgca gtgattaagt tgggcttgaa
    21481 aaaggtcttg gtgcaggaga tgtactctgt tgagaccttg gcgcgcgtgg atatgctctg
    21541 tctggacaag acgggcacca tcacccaagg aaagatgcag gtggaggctg ttcttccact
    21601 gacggaaact tacggtgaag aggctattgc cagcatcttg actagctaca tggcccatag
    21661 tgaggataag aatccaactg cccaagccat tcgccagcgt tttgtgggag atgttgctta
    21721 tcctatgatt tccaatcttc ccttctccag cgaccgcaag tggggggcta tggagttaga
    21781 aggcttgggg acagttttct taggggcacc tgagatgttg ctggattctg aagtcccaga
    21841 agccagggag gccttggaga gaggatcacg tgtcttggtc ttagctctca gtcaggagaa
    21901 attagaccat cacaaaccac agaaaccatc tgatattcag gctctagcct tgctggaaat
    21961 cttggacccc attcgagagg gagcagcaga gacgctggac tatctccgtt ctcaggaggt
    22021 gggactcaag attatctctg gtgacaatcc agttacggtg tccagcattg cccagaaggc
    22081 tggttttgcg gactatcaca gctatgtaga ttgctcaaaa atcaccgatg aggaattgat
    22141 ggccatggcg gaggagacag ctattttcgg acgtgtttcc cctcatcaaa agaaactcat
    22201 catccaaacg ttgaaaaaag cgggtcatac aacggctatg acaggggacg gggttaatga
    22261 tatcttggcc cttcgtgagg cggattgttc tatcgtgatg gcggagggag atccagcaac
    22321 ccgtcagatt gccaatctgg ttctcttgaa ctcagacttt aatgatgttc ctgagattct
    22381 cttcgagggt cgtcgcgtgg tcaataacat tgcccacatc gccccgattt tcttgataaa
    22441 gaccatctat tccttcctgt tagcagtcat ctgtattgcc agtgctttac taggtcggtc
    22501 agagtggatt ttgattttcc ccttcattcc gatccagatt accatgattg accagtttgt
    22561 ggaaggtttc ccaccattcg ttttgacttt tgagcgaaat atcaaacctg tcgagccaaa
    22621 cttcctcaga agatccatgc ttcgtgccct accaagtgcc ctcatggtcg tgtttagcgt
    22681 tctttttgtg aaaatctttg gaacgagtca aggttggtct gagttagaaa tctcaactct
    22741 cctctattat ctcttggggt caattggttt cttatccgta tttagagcct gcatgccatt
    22801 cactctatgg cgagtcctct tgattgtctg gtcagtagga ggcttcctag ccacagctct
    22861 cttcccaaga atccaaaaac tgcttgaaat ttcaaccttg acagaacaaa cgttgcctgt
    22921 ttatggtgtc atgatgttgg tctttacagt gattttcatc ctgactagtc gctatcaagc
    22981 tagaaaataa agaaagactg caatctgtgg attgcagttt ttttaggtac aagattgcca
    23041 gccgaaatgt ggtataataa gaggtaatag agttttggaa agtgagagaa gatgatttca
    23101 aagagattag aattggtagc ttcctttgtg tcacaggggg ctattttact agatgtggga
    23161 agtgaccatg cttatctgcc tatcgagttg gttgagagag gccaaatcaa aagcgctatt
    23221 gcaggtgagg tggtggaagg tccctatcag tctgcggtta aaaatgttga ggctcacggc
    23281 ctaaaggaga aaatccaagt ccgtttagcc aatggcttgg cagcttttga agagactgat
    23341 caagtgtctg tcattaccat tgctggcatg ggtggtcgtt tgattgctag gattttagaa
    23401 gaaggtttgg ggaagttagc taatgtagag cgtttgatcc tccagcccaa taatcgtgaa
    23461 gacgacttgc gtatctggct acaggatcat ggattccaga ttgtagcaga aagaatctta
    23521 gaagaagctg gaaagtttta tgagattttg gtggtggaag caggacaaat gaagctatca
    23581 gccagtgatg ttcgctttgg tcccttcttg tccaaagaag tcagtccagt atttgtccaa
    23641 aaatggcaaa aagaagctga gaagctagag ttcgccctcg gacaaatccc agaaaaaaat
    23701 ctggaagaac gtcaagttct agtagataag attcaagcta tcaaggaggt gctccatgtt
    23761 agcaagtgaa gtgatccaag cttatgaagc cttttgccct caggaatttt ctatggaggg
    23821 agacagctgt ggtctgcaaa ttggcacttt agacaagggt atccaaaggg tcatggttgc
    23881 cctcgatatt cgtgaagaga cggtggctga agccattgaa aagggtgtag acttgattat
    23941 cgtcaagcac gcgcctatct ttcgtcctat caaggacttg cttgccagcc gtccgcaaaa
    24001 tcagatttac atcgacctga ttaagcatga catcgcagtt tatgtcagcc ataccaatat
    24061 tgatatcgtt gaaaatgggc tcaatgactg gttttgtcag atgctaggaa tcgaggagac
    24121 gacttatctg caggaaacag gtccagaacg tggaattgga cgtattggga atattcagcc
    24181 tcagacattt tgggaattgg cccaacaggt caagcaagtc tttgacctag atagccttcg
    24241 aatggtgcat tatcaagagg atgatttgca gaagcctatt tcaagagtag caatttgtgg
    24301 tggaagcgga cagtctttct ataaggatgc tttggcaaag ggggcagatg tctatatcac
    24361 tggtgatatc tactaccata ctgctcagga tatgctgtct gatggcttgt tagcattgga
    24421 tccaggtcac tatatcgaag tgctttttgt ggaaaaaatc gcaacactcc ttactcaatg
    24481 gaaagagaaa aaaggctggg aactagagat tttacctagt caagcatcga ccaatccttt
    24541 ccgtcatatc tagttagaaa gtgaagacaa tgaaaaaagt tgccattatt ggagcaggaa
    24601 ttgtgggagc aacagctgcc tactacctct cgagagaaag tgacctagag gtggccgttt
    24661 ttgaccatga gcaaggtcaa gccaccaagg ccgcagcagg aattatcagt ccttggtttt
    24721 ccaaacgccg taataaagcc tggtacaaga tggcgcgctt gggggctgat ttttatgtgg
    24781 atttattagc tgatttagag aaatcaggac aagaaatcga cttttaccag cgttcgggag
    24841 tctttctctt gaaaaaggat gaatccaatt tggaagaact ttatcaactg gccctccagc
    24901 gcagagaaga atctcccttg atagggcaat tagccattct gaaccaagcc tcagctaata
    24961 aattattccc tggtttgcag ggatttgacc gcctgctcta tgcttctggt ggagcgagag
    25021 tagatggcca acttttagtg actcgtttgc tggaagtcag tcatgtcaag ctggtcaaag
    25081 aaaaagtgac tctgacaccg ttagcatcag gctaccagat tggtgaagag gagtttgagc
    25141 aggttatttt ggcgacggga gcttggttgg gggacatgtt agagccttta ggttatgaag
    25201 tggatgtccg tcctcaaaaa ggacaactac gagattatca gcttgcccaa gacatggaag
    25261 attaccctgt tgtcatgcca gaaggggagt gggatttgat tccctttgca ggtgggaaat
    25321 tatccttggg cgctacccat gaaaatgaca tgggatttga tttgacggta gatgaaccct
    25381 tgctccaaca aatggaggag gcggccttgc ctcattaccc agttttagct agagtgactt
    25441 caagggctga gcgtgtggga atccgtgcct ataccagtga tttctctccc ttctttgggc
    25501 aggtgccaga attggcaggt gtctatgcag ccagtggact aggttcatca ggcctcacaa
    25561 ctggtcctat cattggttac catctagccc aactgatcca agacaaggag ttgaccttgg
    25621 accctgaaaa ctacccaatt gaaaactatg tcaaacgagt aaaaagcgaa taatactcaa
    25681 tgaaaatcaa agaacaaact aggtaactag ccgcgggtta ctcaaagcac tgctttgagg
    25741 ttgtggatag aactgacgaa gtcagctcaa aacactgttt tgaggttgta gatagaactg
    25801 acgaagtcag taaccatacc catggcaagg cgacgttgac gtggtttgaa ttggattttc
    25861 gaagagtata agcattttac tgaaatttta tcaggtagtt taggatggca aatgacattc
    25921 cctgtcaaaa atggtaaaat aagaaaaaat aatctgagaa tcgaggaaaa aagatgcaag
    25981 aaaagatttt ggtaactggt ggtgccggtt ttattggaac ccacactgtt attgaattga
    26041 tccaagcagg ccatcaagtt gttgtggtgg ataatcttgt caatagcaat cgtaagagtt
    26101 tagaagttgt tgaaagaatc acaggagttg agattccttt ctatgaggca gatattcgtg
    26161 acacagatac cctcagagat attttcaagc aggaagaacc gactggtgtc attcactttg
    26221 ctggtttgaa ggctgttggc gaatcaacac gtatccctct tgcctactat gacaacaata
    26281 tcgctggaac tgtcagcctt ttgaaagcca tggaagaaaa caactgtaaa aacatcatct
    26341 tcagttcttc tgcgacagtt tacggggatc cgcacacagt gcccatcttg gaagatttcc
    26401 cactttcagt gaccaaccca tacggtcgta ctaagctcat gctagaggaa attttgactg
    26461 atatttacaa agcagactca gaatggaatg ttgtcttgct tcgttacttt aacccaatcg
    26521 gagcccatga gagtggtgat ttgggagaaa atccaaacgg tattccaaac aatctcttgc
    26581 catatgtgac tcaagtagcc gttggaaaat tagagcaagt gcaagtgttt ggagacgatt
    26641 acgatacgga agatggaaca ggtgttcgtg actatatcca cgttgtcgat ttggctaagg
    26701 gtcacgttgc agctttgaaa aaaatccaaa aaggttcagg actaaacgtt tataaccttg
    26761 gaactggtaa aggttactca gttcttgaaa ttatccaaaa catggaaaaa gcggtgggcc
    26821 gtcctattcc ttaccgcatc gtagaacgtc gcccaggtga tatcgctgcc tgctactcag
    26881 acccggcaaa agctaaagca gaactcggtt gggaagcaga actcgacatc acccaaatgt
    26941 gtgaagacgc atggcgttgg caaagcaagc atccaaatgg atttgaagac taagatgatg
    27001 atttcaatca tcgtcccttg tttaaacgaa gaggaagtac ttcctctttt ttatcaggct
    27061 ctggaagctt tacttccaga tttggaaaca gaaatcgagt atgtctttgt cgatgatgga
    27121 tcaagtgatg ggaccttgga actcttaaag gcctatcggg agcaaaatcc ggcagtccat
    27181 tatatttctt tctctcgaaa ttttggcaaa gaagcagccc tttatgcagg cttgcaatat
    27241 gcgacaggag atttggtggt ggtgatggat gcagacctcc aagatcctcc tagtatgttg
    27301 tttgagatga aaaatgtact agacaaaaat gtagacttgg actgcgttgg gacacggaga
    27361 actagtcggg agggagaacc cttctttcgc agtttctgtg ctgttctctt ttatcgcctc
    27421 atgcaaaaaa tcagcccagt agctctgccg tcgggtgtcc gtgattttcg tatgatgaga
    27481 aggtctgtgg tcgatgccat tttaagcttg actgagtcca atcgtttttc taagggactc
    27541 tttgcctggg tcggctttaa aacccactat ctggactatc caaatgtcga aaggcaggct
    27601 ggcaagacca gttggagttt taggcaactc tttttttact ccattgaagg gattgttaat
    27661 ttttcagatt tccctttgac tatagccttt gtagctggtc tcctatcttg ttttctttct
    27721 ctgctgatga ccttttttgt tgtggttcgg accctcattt tgggcaatcc gacatctggt
    27781 tggacctctc tgatggctgt tattctcttt cttggaggca ttcaactctt gaccattggg
    27841 attctcggta agtatatcag taagatttat ttagagacta aaaaaagacc actttatctt
    27901 atcaaagaaa aaagtgacct tcctgatttt acagaaaaaa ataaagtgaa aagactataa
    27961 ttttacatgg aaatgtgcta aactagaggg agtggattat cgccattgat ttaggcttcc
    28021 tggtttccaa tcagggcgcg agttgggcaa tttttaacag cctctaagac atcctgacta
    28081 ggagaaattt ctttttccag ttggtcaggg tcatcgtaaa aacgcacgat tccattatcg
    28141 tggtaatcaa ataaatcaga ataagtttgg caaagcccac aggcgataca tcgttcaggt
    28201 ataagtgtga ttttcatgtt catatttata ttgtaataag aaaagtaaaa aaaaacaagg
    28261 agtaaggtat ggcaaaagaa ccgtggcaag aagatatcta tgatcaagaa gaatcaagag
    28321 cagagcgtcg gcatcgaaac cacggagggg cggataggat ggctaatcgt attttgacga
    28381 tcctagctag tattttcttt gtaattgtgg tggtgatggt catcgttctc atctatctat
    28441 catcgggggg gagtaatcgc acagcagcct taaaagactt tcatgattct gatgcaagtg
    28501 tagtacaaat ctcatcttca agcagttctc agcctgagca gagttcagag ccagaatcta
    28561 cttctagtag ttcagaagaa gctgctaatc ctgaaggaac gattaaagtt ctcgcaggag
    28621 aaggggaagc agctattgcc gctcgtgcag gaatctccat tgctcagtta gaggccttga
    28681 atcctgggca catggctaca ggatcttggt ttgctaatcc aggtgatgtt ataaaaataa
    28741 aataggagcc gatatgaaaa caattcaaat tgctattgat ggtcctgctt ccagcggtaa
    28801 gagtacggtc gcaaagatta ttgctaagga ttttggattc acctaccttg atacaggagc
    28861 tatgtatcgt gcagcgacct atatggctct taagaaccaa ttaggagttg aagaagtcga
    28921 agcccttcta gccttgttgg accagcatcc aatcagcttt ggacgttcag aaactggaga
    28981 ccagcttgtt tttgtaggag atgtggatat tacccatcct atccgtgaaa atgaagtgac
    29041 caatcatgtt tctgctattg cagcaattcc tgaagtgcgt gagaaactgg tttctctcca
    29101 acaagaaatt gcccagcaag gcgggattgt tatggatggt cgcgatattg gaactgttgt
    29161 attgccgcaa gcagaattga aaattttcct agtagcttct gttgatgaga gagcagagcg
    29221 tcgttacaag gaaaatattg ccaagggaat tgaaacagac cttgaaaccc taaaaaagga
    29281 aattgctgcg cgtgactaca aggatagtca tcgtgagact tctcctctca aacaagcaga
    29341 ggatgctgtc taccttgata caactggttt gaacattcaa gaagtagttg aaaaaatcaa
    29401 agcagaagct gaaaaaagaa tgtaaatgta gaaaagccga tagattgata tcggtttttt
    29461 tctaaatttg tcaaaatgct gattttaggg tataatagtt gctgttcgag aaattttgat
    29521 tttcaaagaa tagtgaatga tgataaggag aaaccatgaa caatttacca aattgtccaa
    29581 aatgtaactc agagtatgtc tacgaagacg gtgccctact ggtttgccca gagtgtgctc
    29641 atgagtggaa tcctgctgaa gttgcagaag tagaagaagg tcttgtcgct atcgatgcca
    29701 acggaaataa attgactgat ggtgatacag taactctcat caaggacttg aaagtaaaag
    29761 gtgcgccaaa agatttgaaa caagggacgc gcgtgaaaaa tatccgcatc gtagaaggcg
    29821 accacaatat cgactgtaag attgatggtt ttggtgccat gaaacttaaa tcagagtttg
    29881 tgaggaagat ttaagcatgt caaaacacta taaacttgta ttttatagcc gtatcttctt
    29941 gtttctagcg gcttttacgg gagtttatct tgaaattact aggcatggtg gttttgggat
    30001 gcttctctat tacacggttc tgtccaacct tttggtaact atttttaccc tttatcttct
    30061 aaaggttatg agccgtgtag gtgaaaattg gcaaaggcca agtctcttgc gcttaaaagg
    30121 tggggtcacc atgagtatca tgattacctg tgtgatttac catttcctct tggcgcccat
    30181 tgcgactaat ttctataccc tagaaaattt cctttgccac tatatcgttc ccatctggtt
    30241 tttagcggat accctctttt ttgacaaaca gggtcaatac aagatttgga atccagcagt
    30301 gtggaccatt ttaccctttc tgtatatgat gtttgctctt tttaatggct tggttctaaa
    30361 actcaatatt ccaaatgcca aggataatcc tttcccttac ttctttttga atgtgaacaa
    30421 gggttggaat gttgtgttta agtggtgtct gattatcttt gttgcctata tggtagcagg
    30481 atttattttc tactttatca agcaaatcaa gagaaagtca tcctaatact cttcgaaaat
    30541 ctcttcaaac cacgtcagtt tccatctgca acctcaaaac actgttttga gcagcctgcg
    30601 gctagcttcc tagtttgctc tttgattttc attgagtata agatacaagg gattcatttt
    30661 cgaatttaag aaggagtcta caaaccaatg aggcttcttc ttttcttgct tatttgataa
    30721 ctatcaaaaa aagtagaaga ttaagagaaa tatagaaaga gatttcatga aacaattttt
    30781 agaacgggcc agcattttgg ctctctccct cgttttgatt acctcctttt ccatttcgag
    30841 tgccctacca gccatgtttg actattatca gggttattct aaggaacaaa ttgagctctt
    30901 ggtgagcttg ccttcctttg gaatcatgat gatgttactg ctaaatggtt tcttagaaaa
    30961 aatatttcct gagcgcttac agattagttt gggcttgctg attttatcat tgagcggtac
    31021 agctcccttc tggtaccaag cctatccctt tgtctttgga acacggcttc tctttggttt
    31081 gggtcttggg atgatcaatg ccaaggccat ttctattatc agtgaacgct accaaggaaa
    31141 aaggcgaatt cagatgttag ggctacgcgc ttctgcagag gtcgttggag cttctctcat
    31201 taccttggcc gtcggtcagt tgttggcctt tggttggaca gctatctttc tagcctatag
    31261 tgctggattt ttggtgctgc ccctttatct gctctttgtc ccttatggaa aatcaaagaa
    31321 agaagtcaag aaaagagcga aggaagcaag tcgtttaact cgagaaatga aaggcttgat
    31381 ttttacctta gctatcgaag cggcagttgt agtttgtacc aatacagcta ttaccatccg
    31441 tattccaagt ttgatggtgg aaagaggatt gggggatgcc cagttatcta gttttgttct
    31501 tagtatcatg cagttgatcg ggattgtggc tggggtgagt ttttctttct tgatttctat
    31561 ctttaaagag aaactgctcc tctggtctgg tattaccttt ggcttggggc aaatcgtgat
    31621 tgccttgtct tcatccttgt gggtggtagt agcaggaagt gttctggctg gatttgccta
    31681 tagtgtagtc ttgacgacgg tctttcaact tgtctctgaa cgaattccag ctaaactcct
    31741 caatcaagca acttcatttg ctgtattagg ctgtagtttc ggagccttta cgaccccatt
    31801 cgttctaggt gcaattggct tactaactca caatgggatg ttggtcttta gtatcttagg
    31861 aggttggttg attgtaatct ctatctttgt catgtaccta cttcagaaga gagcttagga
    31921 ttgattccta agtttttctt ttgatactca atgaaaatca aagagcatag ttgattgagt
    31981 ttggaatagt acgctgtagc tgctaaaata tttctagaaa ttaatttgac tttcctaata
    32041 gagttgttca tatcttattt caatctacta tagaaagcta gccgcaggtt gctcaaaata
    32101 gtgttttgag gttgcagatg gaaactgacg tggtttgaag agattttcaa gagtataaga
    32161 tttttatacc catacaatat ttgttttagg tccttgttta cagactaatt tcttgataaa
    32221 atagaggtta tgacgagata taaagcaact atttcctatg atggttatgc ctttgctggc
    32281 tttcagcgcc agcctcatgc gcgtagcgtt caggaagaaa ttgaaaaaac cttgaccaga
    32341 ttaaataaag ggcaaaccat tactgttcac ggtgctggta ggacagatag tggggttcat
    32401 gccctgggac aggtcattca ttttgacctg ccttatcaga tggatgagga gaaactccgt
    32461 tttgccttgg atacccagtc tcctgaagat attgatgtga tttcgattga gcttgtggca
    32521 gctgattttc attgccgtta tgccaagcat agcaagacct atgagtttat tgtggataga
    32581 ggacgtccca aaaatccgat gcgccgtcac tatgccaccc actttcccta cccactcgat
    32641 gtggaacgaa tgcagattgc aatcaaaaag ctagagggaa cccatgattt taccggtttt
    32701 acagcctctg ggactagtgt agaggataag gttcgcacca tcacagaagc tagtttaata
    32761 gtcgatgaga caggacaatt tttgaccttt accttttcag gaaatggttt cttgtataaa
    32821 cagattcgca atatggtggg gactcttctc aaaatcggta acaaccgcat gccagtagag
    32881 cagattgatc tcatcttgga gaagaaggac aggcaacttg caggtcccac tgcagcacca
    32941 aatggtttgt atttaaagga gattcgttat gaagaataat cgtattttag cactttctgg
    33001 aaatgatatt tttagtggtg gtggactgtc agctgatttg gctacctata ccttgaacgg
    33061 cttgcatggg tttgtagcag tgacttgttt gacagccttg acagaaaaag gatttgaagt
    33121 ctttccaact gatgatacca tttttcaaca tgaattagat agcttgcgtg atgtggaatt
    33181 tgggggaatt aagattggtc ttctccctac tgtcagtgtg gctgagaagg ccttggactt
    33241 tatcaaacaa cgcccaggag tacctgtggt gttggatcct gtcttggtct gcaaggaaac
    33301 gcatgatgta gctgtcagtg agctctgcca agagttgatt cgctttttcc cttatgtcag
    33361 tgtgattacg cctaatctcc cagaagcaga attattatcc ggtcaggaaa ttaaaacctt
    33421 ggaagacatg aaaactgcag cgcagaaatt gcatgattta ggagcgccag cagtcattat
    33481 caagggaggc aatcgtctta gtcaggacaa ggctgtggat gtcttttatg atggacagac
    33541 ctttactatc ctagaaaatc cagttatcca aggccaaaat gctggtgcag gttgtacctt
    33601 tgcctctagc attgccagtc acttgattaa aggtgataaa cttttgccag cagtagaaag
    33661 ctctaaggct ttcgtttatc gtgctattgc acaagcagat cagtatggag taagacaata
    33721 tgaagcaaac aaaaacaact aaaatcgccc ttgtatccct attaaccgcc ctttctgtgg
    33781 ttctaggtta tttcttaaaa atcccaacac ctacaggaat tctaactctt ttagatgctg
    33841 gtgtcttctt tgcggccttt tactttggta gtcgtgaagg agcggtagtc ggaggactag
    33901 caagtttctt gattgacctc ttatcaggct accctcagtg gatgttcttt agcttggtca
    33961 accatggctt gcagggattt ttcgcaggat ttaaaggaaa aagtcagtgg ttaggcctta
    34021 ttttagcaac tattgccatg gtaggaggct acgccttggg ttctactttg atgaatggct
    34081 gggcagcagc cctcccagaa attctaccaa atttcatgca aaatatggta gggatgattg
    34141 taggatttat tcttagtcaa agtatcaaga agattaagta atactcttcg aaaatctctt
    34201 caaaccacgt cagcttccat ctgcaacctc aaaacactgt tttgagcagc ctgaggctag
    34261 ctttctagtt tgcactttga ttttcattga gtataaagag gttgagtaaa aagtatttca
    34321 aataagaaaa acgcatagta tcaggtgttg aatgaactgc cccccaaaag ttagacagaa
    34381 aaaatctaac ttttggggtg ttttattatg aaactaactt atgatgataa agttcagatc
    34441 tatgaactta gaaaacaagg atatagctta gagaagcttt caaataaatt tgggataaat
    34501 aattctaatc ttaggtacat gattaaattg attaatcgtt acggaataga gttcgtcaaa
    34561 aaaggaaaaa atcgttacta ttctcctgat ttaaaacaag aaatgattaa tcaagtctga
    34621 catgaaggct ggactaaaga tagagtttct cttgaatacg gtctcccaag tcgtacgata
    34681 cttcttaact ggctagcaca atacaggaaa aacgggtata ctattgttga gaaaacaaga
    34741 gggagagtac ctaaaatggg acgtaagcca aaaacgagac ctgaagagag gacagaatta
    34801 gaatatcttc aagcagaaaa tgagtacctg agagcggaga atgccatcct aaaaaagtta
    34861 agagaactcc gattgaagga ggaaaaagag aaagaagaaa gacagaaatt gttcaagaat
    34921 taatgactga gttttcgtta gttcttcttc taaaatccat taaactagct cgttggacct
    34981 actactatca cttgaaacag ctagacaaaa tagataagga ccaagagctt aaagctgaaa
    35041 ttcaatccat ttttatcgaa cacaagggaa attatactta tcgccgggtt catttagaac
    35101 taagaaatcg tggttatctg gtaaatcata aaagagttca aggcttgatg aaagtactca
    35161 atttacaagc taaaatgcga cagaaacgaa aataaaggag acgttggtaa gaagacagag
    35221 aatctcattc aaggccaatt tgaaggctct aaaacaatgg aaaagtgcta cacagatgtg
    35281 acagaatttg ccattccagc aagtactcaa aagctttact tatcaccagt tttagatggc
    35341 tttaatagcg aaattatcgc ctataatctt tcaacttcac ccaacttaga acaagtacaa
    35401 acaatgttgg aacaggcatt cacagagaag cactacgaga atacgattct ccatagtgac
    35461 caaggctggc aataccaaca cgattcttat catcggttcc tagagagtaa gggaattcaa
    35521 gcatccatgt cacgcaaggg aaacagccca gacaatggta tgatggagtc cttctttggg
    35581 attctgaaat cggaaatgtt ttacggttat gagaagtcgt ttcagtcgct taagcaattg
    35641 gaacaagcca ttatagacta tattgattat tacaacaaca agagaattaa ggtaaaacta
    35701 aaaggactta gccctgtgca atacagaact aaatccttcg gataaattaa ttgtctaact
    35761 ttttgggggg cagttcattc tacacccact attatgcgtt ttattgtggg aagattttcc
    35821 tcattttcta gtgaaattga actttttcta acatcttttg atagatgact tcttaatcaa
    35881 ttatcatttc cattgttgtt tcttggggtt tcatcccttg tctttgataa aagcggagag
    35941 caccctcgtt atcattccag acacttaggg ttagattatt gcagccaatt tctttagcat
    36001 aatagcagaa aaatgacaat aaaatagccc ctagatattt tgaaaggaaa gcataggggc
    36061 tatatagttt aatcaaaata aagcaaatct ttgctggtgc ttgtaaagag gtcgaagcca
    36121 tccttggtaa caacaccgca gtcttcaata cgaacaccga ctttaccagg gatatagata
    36181 cctggttcaa cagagaagca catgccttct tcgatgacca tgtcgtttcc ttccatgata
    36241 gatgggaatt catggacatc cataccgata ccatgcccga gacggtggtt gaagtactca
    36301 ccataaccag ctttttcgat gacctcacgg gcagcgcggt ccacttcatg agcagtcaca
    36361 cctggcttga taaagtcaag agcagcttgt tgggcttcaa gagtcaagtt gtaaatatct
    36421 ttcttgaatt ggtctggttt gccgacagcg actgtacgag tcatatctga cgcatagcca
    36481 ttgaccagaa cacccaggtc aaagaggaga agagcatcat tttcaacctt attagctgct
    36541 ggaatgccgt gtggattcgc agcattatca ccagtcaaga ccatggtatc aaagctcatt
    36601 tcataacctt cacgtttcat ggcaaagtcg atttgtgcga tgatatctgt ctcagtctta
    36661 tcaagagaaa tattgtcaaa accaacatga acagccttgt cagcataaag acctgcaacc
    36721 atcatttttt gcacttcatc agctgatttg atgaggcgca tgcgttggat acgaggagtg
    36781 aggttgtcaa actcagcagt ctcaaaaact gttttcaaac catggtattt ggtcaagatg
    36841 agattgtcaa actcaacagc gacacgtttg aagtcaagtt gtggaagagc atgtttgatt
    36901 ttttgccatg gattttcaga atcgacatag cccactactg ggaaggaaac ggtgctactt
    36961 gcacgttcta cttcaagagc tgggacaaag aggagaggtt cctgatccgc taggacaaag
    37021 aggaacattt ggcgttcatg gggatcactg taaaaaccag tgaggtaatt gattgtgacg
    37081 gggtcagata cgacagcgac gtctagtttt tctgattcaa gatatgttag gatttgttgt
    37141 aatttagaca tatgctacct tctttctacc cctctatttt ggcaaaaaat gagagaaaat
    37201 gcaagggaga agggtaaaag aaaagacaaa aaactccgac aagataacgg agttctttga
    37261 tatcattttc ctttgtttaa gaaaagtcag ccttgctttt gacgtcactg tactcttcag
    37321 cgatttcttg gctgaggata tcctcatagg cagggagttg gatatcctga ccatatttct
    37381 tcttgagggc agtagtaact aggcgataag ccagattggc attacgagag aggataggcc
    37441 cgtggaagta ggaaccaaag acattcttat aatgaacccc ttcaccgacc ttttcttcgt
    37501 tgtttccatt tccatagaca acctgtccca gcggtttttg gtcatcagag aggaaggtac
    37561 ggccttggtg attttcaaat ccatagtagg tttcatcgaa atcttcattg tgaatcttga
    37621 tgtcaccgat aaaacggtta ttggtctggt tgagcgtgta gtgtcccatg acccctagcc
    37681 cttcgatacg ttttcctgaa gcttcaacat aatattgacc caatagttgg aaaccaccgc
    37741 agatagccag aactacaccg tcgttttgga tgtagttgtc aatgctctct tttttagcag
    37801 gtaggtcgtc tgcaatgata ctttgttcaa agtcttgacc accaccgaaa aaggcgatgt
    37861 cgtagtgatt ttcatcaaag tcatcatgga gagaaacgat gtcaacggtc acatgggctc
    37921 ccagtttttc agccacatac ttgagcatga ggatgtttcc attgtccccg taggtattca
    37981 tgagatttcc gtagaggtgg gcaatgttga gctgataggg gtaattgcca tcttttgagg
    38041 aaagtgaagt ataaaccatt agttcatctc ctttctaaca atctgacgac tagccagcag
    38101 ttcacgaaat tccagcatgg cagtataagt tgccagaata taggcatgct tgcagtcttg
    38161 attctcaatg gtcttgagaa cttgctccag attactcgtt tcagtgattt tctcagctgg
    38221 atagccagtc actcggaggc gacgagcgat ttcagaatga cgaacaccac cagcgttgat
    38281 ttcaggaatg tccatgtcag tgatttgttc aaagtctgca tcccagatcc agctagtgtc
    38341 aattccatct gcatagttgg cattaaggag gacagatagg ctaaatggat aaggtgctag
    38401 tttgatcatt tcgatagctt gggttgcacc gactggattt ttaatcaaga caagggtaca
    38461 ttccttgtca ccgatatgaa aggtttcttg gcgtccaaag acagcacggc tcttgtcaaa
    38521 tccctgtttg atgagttgcg aatcggcacc taggaaacgg gcgatggcca cagcagctag
    38581 ggcgttatag atattataga gcccgccgat ttggataccg tattcttggc cgtctatgac
    38641 aaagcgagag cgattgttgg tcaactcaac cagttttgtc aaacgatagt cgagatcagg
    38701 acgtttgcat ccacaacctt cacagatata ggcacccaag tttgcatagg tattatgctc
    38761 atatttgagg atgccttggc agtcaggaca gagaatccct tcggtattgt agtgagccag
    38821 ttgggctggt cctttttcca agtcaaaacc aaaatactct atagggtttg gaatagttgg
    38881 cttgtagaaa agtggactgt ctccgttaag gagaacagta gcagttggaa ctttccgaat
    38941 ggcttccaat atcatgttat aggtagtata gatttcaccg aaacggtcca tctggtcacg
    39001 gaagatatta gtaatgacaa aaagactagg ctggatatag tcacagatac gagatagact
    39061 ggcttcgtca atttcgagga cggcaatatt tttcccagtt tttgaagatt tggctgttag
    39121 gaaggttgtt gcaatccctg taatcatgtt ggcaccgctt gggttggtta gaacttgacc
    39181 ataaacctct tttaaaatgc cgacagtgag ggcagttgtc agggtttttc catttgttcc
    39241 agtgacaacg acaatctcgt agttcttagc taggttttgt aaaatatctt tatcaaattg
    39301 aagggcgact ttacctggga gcgtacttcc acggccaaga cggcttaaaa tgaagtggga
    39361 agaacgccca gcaagaaggc ccaaagtagt ttttaagttc atgtttctat tatatcataa
    39421 aagaggagaa agacagattt gagattccgc agaagtaaaa acagcccaaa gagggctgtt
    39481 cgatacgatt aaatctcaac ttaaatcgca aacaagtcat tcaagttctc agccaaggtt
    39541 gggtgagtga agatttgttt tgtgaagtaa gtgtaaggaa tcttgttgtc catagcaaca
    39601 gtgatgatgt tgatgatttc ttgagaacct tctgagaaga tgcttgctcc aagaatttct
    39661 tttgtttcag tattgacaac agctttgaag gcaccgcgaa ggtctccatt tacgtgacca
    39721 cgaggcattg ctgcaacggg gatttcctta acagcgtatg gaagtttcaa atcagctgct
    39781 tggctttcag tcaaaccaac ttgtgaaagt gcaggtgtga tgaacatagt atttggcaca
    39841 ttgagacggt cttcaagtgt gtagctgcca tctccagcaa ggtagctgta aacaacacgg
    39901 aagtcatcaa gtgaaatgta agtaaattga aggccaccgt tgacatctcc aactgcaaag
    39961 acaccaggaa cgtttgtttg acagtgtttg tctactttaa tagcaccacg ttcagttagt
    40021 tcaatatctg tattttcaag ttgaagtggt tctacatttg gtttgcgtcc agttgcgtag
    40081 agaagggcgt cgaaacggta agtttcgtct tcagttacga caagcacttg gtcaccatcg
    40141 tttttgattt cagtagtatg gatattttga agcaattcaa tgccatcttc ttccatgtat
    40201 tgtttagcaa gagctgcgat ggaaggttct gcacgaggta ggaatgtatc caaggcatct
    40261 aggactgtga ccttgcttcc aagtttgttg taaaggccgg caaattcaag accgatattt
    40321 ccgccaccaa ggattccaag tttttcaggt aatttgtcca agctttggat acctgttgag
    40381 tcaaagatgt ttttgcttgt agcaagtcca gggattggca agacgtttga aacagcacca
    40441 gtgttgatga cgattgtttc agcagtcagt tctttctttt catcaccagc ttggatttcg
    40501 atgactttat ttgaaaggaa gtgagcttcc gcatcaaaga tatctacgcc tgtaccagca
    40561 acagtcgcat agtttttacc gttgaggcga ccagtgatcg tgtttttagt agcaatgact
    40621 tcttcaaaag acaagtcctt ttcagcagca actagcaagg ttttagttgg gatacaacca
    40681 atgttgatac aagttccacc gtacatagcc ttgctgcgtt caacgagggc aacttttttg
    40741 ccagctgaag ccaatttacc tgctagtgtt ttaccagctt taccaaatcc aataacaatt
    40801 aaatcgtatg ttaacattta tcttcctcct attcgaattt cattctagca caagaaaaaa
    40861 acttttgcac aattctgcta cggaaaagac agaaaaagat aaaaacgctt tccagacaaa
    40921 aaactagtaa ttttttgaca agcttgataa actaaaatcc tatctatttt tatataagat
    40981 agaataaatg ttcgaaaata ggaggtttta tgaaatcgaa tcgttatatt attgcctttg
    41041 ctggggtcat tttacactta atgctgggtt cgacttatgc ttggagtgtt tatcgtaacc
    41101 ctattattga aaaaacggga tgggatcagg cttctgttgc cttcgccttt agtctagcaa
    41161 tcttttgttt gggtttatcg gctgcattta tggggcgttt ggtagaaaaa tttggtccga
    41221 aagtcatggg gagtctatct gcttttctat acgcaggtgg aaatatctta acaggatttg
    41281 caatagaccg tcaggaactg tggttgttgt atctagctta tggcatttta ggtgggcttg
    41341 gtttgggagc aggctatatt acccctgtgt caacgattat aaaatggttt cctgataaac
    41401 gtggtctcgc aacaggttta gcgattatgg ggtttggttt tgcttcttta ttgactagtc
    41461 ccatagcgca acaccttatc gcaggggtag ggattgtaga aactttttat attttaggag
    41521 caagttactt tattatcatg ctcctagctt cacaattcat taagcgtcca aatgagcaag
    41581 agcttgcaat tttatcttct tcagggaaag aaaaaacagc ctctttgacg caaggaatgg
    41641 ctgcaaatca ggctctaaaa agcaatcggt tttatatgct ttggattatt ttctttatca
    41701 acatagcttg tggtttaggc ttaatttcag cggcatcgcc aatggcacag gagatggctg
    41761 gcttgtctac aagtcatgca gcagtaatgg tgggtgtttt ggggattttc aatggatttg
    41821 gtcgcttgct ctgggcgagt ttgtctgact atatcggtcg ccctctaacc tttagtatat
    41881 tactgcttgt caatcttttc ttttctctct cactttggct ctttacagat tccgttttat
    41941 ttgtagttgc tatgtctatt ttgatgactt gctatggagc tggtttttct ttgattccag
    42001 cttatctcag tgatattttt ggaaccaagg aattggccgc tctgcatggt tatattttaa
    42061 cagcttgggc aatggctggt ttagcgggac ctattttatt agcagagact tataaaatgg
    42121 ctcattcgta cacacaaacc ttgttcgttt ttctcatttt atacagtatc gccttggctt
    42181 tgtcttatta tctaggtcgt tcaatcaaaa aagaaagtca aaaaccgctt acatgatttt
    42241 ctgtagaaat ggggctatct tttgctctgg tcttgtgtta tactagatag gttgcaaaga
    42301 aaacagtact tttcttttgt ggaaaagaag caacacgatt ttatatctag ttatatgaaa
    42361 atacgtcata aaaagaaaag tataaactaa gccctatagg gtggcttcgc accaccttta
    42421 gaaagaagaa taacgtgaaa tttaatgaat taaacttgtc tgctgatttg ctagcagaaa
    42481 ttgaaaaagc tggttttgta gaagctagtc ctatccaaga acaaactatt cctttggccc
    42541 ttgaaggcaa ggatgttatc ggtcaagctc agactggtac aggaaaaact gcagcctttg
    42601 gcttgcctac ccttgaaaaa atccgtacag aagaagcgac tatccaagct ttggtcatcg
    42661 ctccaactcg tgaactagct gtccaaagtc aagaagaact cttccgcttt ggtcgtagta
    42721 agggagtcaa agtccgttca gtatatggcg gatcaagcat tgaaaaacaa attaaggctc
    42781 ttaaatctgg tgcccatatc gtggtgggaa ctccaggtcg cctcttggac ttgattaaac
    42841 gcaaggcctt gaaattacaa gacattgaaa cccttatcct tgacgaagcg gatgaaatgc
    42901 ttaacatggg cttccttgaa gacatcgaag ccattatttc ccgtgtacct gagaaccgtc
    42961 aaactttgct tttctcagca actatgccag atgccatcaa acgtatcggt gttcagttta
    43021 tgaaagcccc tgaacatgtc aagattgcgg ctaaggaatt gacaacagaa ttggttgacc
    43081 agtactatat ccgtgttaag gaacaagaaa aatttgacac catgactcgt ctcatggatg
    43141 tggcacaacc agaactcgct attgtatttg gtcgtaccaa acgccgtgtg gatgaattga
    43201 ctcgtggttt gaaaattcgt ggcttccgtg cagaaggaat tcatggcgac ctagaccaaa
    43261 acaaacgtct tcgtgtcctt cgtgacttta aaaatggcaa tcttgatgtt ttggttgcga
    43321 cagacgttgc agcgcgtggt ttggatattt caggtgtgac ccatgtctac aactacgata
    43381 ttccacaaga tcctgagagt tatgttcacc gtatcggtcg tacaggtcgt gctggtaagt
    43441 caggtcaatc tattactttt gttgctccaa acgaaatggg ttaccttcaa atcattgaaa
    43501 acttgactaa gaaacgcatg aaaggtctca aacctgcaag tgtagaagaa tccttccaat
    43561 caaaaaaaca ggtagctctc aagaaaatcg aacgtgattt tgcagatgaa accattcgtg
    43621 ccaactttga gaaatttggt aaggatgctc gcaaattggc tgctgagttt actccagaag
    43681 aattggcaat gtatatcttg agtctgacag tccaagaccc agatagcctt ccagaagtgg
    43741 agattgcacg tgaaaaacca ctaccgttta aaccatcagg taatggtttc ggtggtaaag
    43801 ctaagggagg tcgtggaggc cgtcgtgggg acgaccgtcg agagcgtgat cgccgtggca
    43861 atggtcgccg tgatgagttc aaaaaaggaa gtcgtggcaa cgatcgtttt gataaggaaa
    43921 aacgttaccg taaggataat aaaaaaccac gcaatacttt aaacgaaaag caaacaggct
    43981 ttgttattcg taacaaaggt gataaataag aaaaagcggt tcaaaatgaa tcgctttttt
    44041 atataaggag accgaatgtg aaaaagacaa ataaaattat tatattatct tttataatat
    44101 tatgatacac aaaaataggc aacttgtcaa tagaaaagga acaatttaat taaaaatatt
    44161 ctggttttta attaaattgt aagtcaatgc aagtcaataa ggaattttca gataattatt
    44221 gaaaaagaga cgtttttatg ttataatgat agcgattaaa ttcgaggtga aaaatggcac
    44281 atttattaga aaaaactaga aaaattacat caattttgaa acgctcagag gagcagttgc
    44341 aggatgagct tccttacaac gctattacgc gtcagttagc ggatattatt cattgcaatg
    44401 cctgcattat caatagtaag ggacgtctgc ttggctattt tatgcgttat aaaacaaata
    44461 cagatcgcgt agagcaattc ttccaaacta agattttccc agatgactac gttcaagggg
    44521 ctaatatgat ttacgaaaca gaagcaaact tacctgttga gcatgatatg agtattttcc
    44581 ctgttgagag tagagatgat tttccagatg gcttgacgac tattgcaccg attcatgtat
    44641 cggggattcg ccttggttct ttgattattt ggcgtaatga taaaaaattc gaagatgagg
    44701 acttggttct tgttgagatt gccagtaccg ttgttgggat tcagcttctt aacttccaac
    44761 gtgaagaaga tgagaaaaat attcgtcgtc gtactgctgt caccatggcg gtcaataccc
    44821 tttcttactc cgaactccgt gctgtttcag caattttagg ggaattaaat ggaaatgaag
    44881 ggaagttgac tgcgtcagtg attgcagatc gtatcggaat cactcgctct gtgattgtca
    44941 atgctcttcg taaacttgag tctgcgggga ttattgaaag tcgctcactt ggaatgaagg
    45001 gaacctatct taaggtcttg atttcagata tttttgaaga agtgaagaaa agagattact
    45061 aatgacaaag gctttaattt cgattgatta tacagaagat tttgttgctg atagtgggaa
    45121 actaacagca ggtgctccag ctcaggcgat ttcggatgcc atcagcaagg taactcgatt
    45181 agcttttgaa cgaggagatt atatcttctt tactattgat gctcatgaag aaaacgattg
    45241 tttccatccg gaaagtaagt tatttcctcc tcataatctg attgggacga gtggacggaa
    45301 tttatatgga gatttgggga tcttttatca agagcatggt tcagacagtc gtgtcttttg
    45361 gatggataaa cgccattact cagctttttc agggactgac ctagatattc gtttgagaga
    45421 gcgtagagtg tctaccgtta tcttaacagg tgtcttgacg gatatctgtg tcctacatac
    45481 agctatagat gcctataatc taggatatga catcgagatt gttaaaccag ctgttgcttc
    45541 catctggcct gaaaatcatc aatttgccct aggtcatttc aaaaatacac tcggagctaa
    45601 gttagtagat gaaaatctaa atgaactttc tgagtaacct ggttggtgaa aatgaaaaaa
    45661 tttgaaaaaa gtgttgacaa gcatcccaaa agttgatata ctagtaaagt aatcgacgcg
    45721 gggatggcgg aattggcaga cgcgcaggac taaggatcct gtgaccgctt taggtcgtga
    45781 gggttcaagt ccctctctcc gcataggaaa agagctagct aaagctagct cttttgtatt
    45841 tctctaaaaa attagaatcc agtcaaatgt agtggagaat cagcgaaaat atgggaaaac
    45901 tgatccttgt ttctgctttt gtcatagaag aataataaag attatcttct tcaaattctc
    45961 cgatattctc taaagttttg tgcaagttgc acagaacttt tttatttttt tggtcatctt
    46021 gccatagaaa tataaagcgt tttcatctat aatataatta tcaaaagata aaaggagttc
    46081 acctcatggt agaattgaat cttaaaaata tttacaaaaa atatccaaac agcgaacact
    46141 attcagttga agatttcaac ttgaacatca aagataaaga atttatcgtt ttcgtaggac
    46201 cttcaggatg tggtaaatca actacactcc gtatgattgc tggtcttgaa gacattacag
    46261 aaggtactgc atctatcgat ggcgtggttg tcaacgacgt agctccaaaa gaccgtgata
    46321 tcgccatggt attccaaaac tacgctcttt acccacacat gactgtttat gacaacatgg
    46381 ctttcggttt gaaattgcgt aaatacagca aagaagacat taacaaacgt gttcaagaag
    46441 cagctgaaat acttggattg aaagaattct tggaacgtaa accagctgac ctttcaggtg
    46501 gtcaacgtca acgtgttgcc atggggcgtg cgattgtccg tgatgcgaaa gtattcttga
    46561 tggacgaacc tttgtcaaac ttggatgcca aacttcgtgt atcaatgcgt gctgaaatcg
    46621 ctaaaattca ccgtcgtatc ggagctacaa ctatctatgt aactcacgac caaacagaag
    46681 cgatgacact tgcagaccgt atcgttatta tgtcagctac taagaaccct gctggtacag
    46741 gtactatcgg acgtgtagaa caaatcggta ctcctcaaga agtttacaaa aatccagtta
    46801 acaaatttgt tgcaggattc atcggaagcc cagctatgaa tttcatcaac gtgaaattgg
    46861 ttggtagcga aattgtttct gacggtttcc gtttgaaagt gccagaagga gcattgaaag
    46921 ttcttcgtga aaaaggctac gaaggaaaag aattgatctt tggtatccgt ccagaagacg
    46981 tgaatgcaga acctgctttc cttgaaacat tcccagactg tgttgtaaaa gcgactatct
    47041 ctgtatcaga actgcttggt tcagaatctc acctttactg ccaagttggt aaagacgagt
    47101 ttgttgcaaa agttgatgct cgtgactact tgcaaacagg tgcaacagtt gagcttggat
    47161 ttgacttgaa caaagcacac ttcttcgatg tagaaactga aaaaacaatc tactaaaata
    47221 aataaaattc aaagcactac aagaaaagat atctctttat caattgtagt ggagagatat
    47281 cagttaatct agggagagaa acaaaatgct tctctccttt ttgctaggga agtcatatta
    47341 tgcatctata ttgtgatgct ctttaatact cttcgaaaat ctcttcaaac cacgtcaacg
    47401 tcgccttgcc gtacgtatga ttactgattt cgtcagtttt atctgcaacc tcaaagatgt
    47461 actttgagca gcttacggct agtttcctag tttgctcttt gatttccatt gagtattatt
    47521 tgtgggtacc atctacaagt gaagctatat gcgtaaacta cgtgagcaat tgaattcgaa
    47581 ctagagaggt aataataaat ttatgctata gttatggtga cttgtatgct tttgattcta
    47641 gtttatcaaa taatagatta gaattgtcag ataatatcat tttgtgttat aatgaagaaa
    47701 aaacagaggt gttcaaatgt cagaagcagg tcataagttt ttagcaaaat tggggaaaaa
    47761 acgcttacgt ccaggtggaa agcgtgccac agattggtta attgcagaag gaggattttc
    47821 aaaagaaaag agaatactag aggttgcgtg taatagggga actacagcaa ttgagttggc
    47881 acagcgtttt ggttgcaaga taactgctgt tgatatggat gctcaagctt tagaagtggc
    47941 taaaaaatct gctggaacgg caggtgttgc tcatttaatc agttttgaaa gagcaaatgc
    48001 aatgaaactt ccttatcaag atgctagttt tgatattgtt ataaatgaag ctatgctgac
    48061 tatgcaagcc gatcaagcta agaaaaaatg tgtaatggaa tatctaaggg tattaaaacc
    48121 tggaggtctt ctcttgacac atgatgtact tcttaaggaa gctaaagagt ctatcagaca
    48181 ggaattatca caagcaattc atgtaaatgt aggtccttta actcaagatg gttgggaaca
    48241 ggtgatgata gaatcaggtt attgtgatgt gaaagcattg actggtgaaa tgacattaat
    48301 gaaattatcg ggtatgattt atgacgaagg tttgctagga actttgaaaa tttgtgtaaa
    48361 tgcttgtaaa aaggagaata gaaagcagtt tttaactatg tataaaatgt ttgctaagaa
    48421 taaacagaaa ttgggcttta ttgcgatggc tagttataaa tcgtcaaaac gttagataat
    48481 tattgaagtt aacttttcct tttttctttc ttaaaaaata tgctataata gagagtaaaa
    48541 aactttgaaa gaaagaaaaa agatgaattt aaaagattac attgcaacaa ttgaaaatta
    48601 tccaaaggaa ggcattacct tccgtgatat tagtcctttg atggctgatg gaaatgctta
    48661 tagctacgct gttcgtgaaa tcgttcagta tgctactgac aagaaagtcg acatgatcgt
    48721 gggacctgaa gctcgtggat ttatcgtggg ttgtccagtt gcctttgagt tgggaattgg
    48781 ttttgcgcct gttcgtaagc caggtaaatt gccacgcgaa gttatttctg ctgactatga
    48841 aaaagagtac ggtgtcgata ccttgactat gcacgcggat gccattaagc caggtcaacg
    48901 tgttcttatt gtagatgacc ttttggcgac aggtggaact gttaagacaa ctatcgagat
    48961 gattgaaaaa cttggtggtg ttatggcagg ttgtgccttc cttgttgaat tggatgaatt
    49021 gaacggccgt gaaaaaattg gtgactacga ttacaaagtt cttatgcatt attaatgaaa
    49081 acagtcccta gggctgtttt ctctacacta ggatataaaa atagactata actagttaga
    49141 gaaaaactat aattgaaaac tatatcttct tacagtataa taaaaggact aagtgtttga
    49201 gatttgtctt caaacatatg caattattcc tgaaagagta cagttaggag agggttatgc
    49261 cgattcgaat tgataaaaaa ttgccagctg ttgagatttt acggacagag aatatctttg
    49321 tcatggatga tcaacgtgct gcccaccaag atatccgtcc tttgaagatt ttaattttaa
    49381 atctcatgcc acagaaaatg gtcacagaga cccagttgtt gcgccacttg gctaatacac
    49441 ccctacaact ggatattgat tttctctata tggagagcca ccgttctaaa acaactcgtt
    49501 cagagcacat ggagaccttc tataaaactt ttcctgaagt caaggatgag tattttgatg
    49561 ggatgatcat cacgggtgct ccagttgagc atttaccatt tgaggaagtg gactattggg
    49621 aggaatttag acagatgctt gagtggtcta agactcatgt ctattcgacc cttcatatct
    49681 gttggggggc tcaggctggg ctttatctgc gctatggtgt agaaaaatac cagatggaca
    49741 gtaagctatc aggtatttat cctcaggaca ccctaaaaga gggtcacctt ctatttagag
    49801 gctttgatga tagctatgta tcccctcatt cacggcacac ggagatttct aaggaagagg
    49861 tcttaaacaa gaccaatctc gagattttat cagaaggacc tcaggttggg gtttctattt
    49921 tggccagtcg tgatttacga gaaatttata gttttggtca tttggagtat gaccgtgata
    49981 ccttggcaaa agagtatttt cgagatcgtg atgcaggttt cgacccacat attccagaaa
    50041 attactttaa agatgatgat gttaatcagg taccttgtct ttgttggtct tcatctgcag
    50101 ccctcttttt cagtaattgg gtaaaccatg cggtctatca ggagacgcct tttgactgga
    50161 gaaagataga agatgatgca tctgcatata ggtatttata agaggaatta tgacatattt
    50221 agacgctttt aaatcaggta ccttggtttt accgagtgcc ctgctcttgc attttaagga
    50281 actctttcct tctagcgacg attttctggt ttggcaattt ttctatttgc aaaatacgac
    50341 aggcttagaa gaaatatcgc caagccagat tgctgaaagg attggcaagg aaatttcgga
    50401 tgtcaaccag tccatttcta atctgacgga aaggggactg ctccagtatc gtactatcga
    50461 attaaatggc gaaattgaat tgctctttga tgctagtttg gccttggaac gtttggatga
    50521 cctgtttgga gcagttcatt caagttcaga ccagctaaca ccacaaaacc agctcaagga
    50581 tttggtggaa accttccagc aggagttggg acgattgttg acgccttttg agattgagga
    50641 tttgaccaag acactaaagg aagatggaac cagtgctgac ttgattaagg aggctcttcg
    50701 tgaagctgtt ttgaacggaa aaccaaactg gaagtacatt caggcgattt tgagaaactg
    50761 gcgccatgaa ggaatcaaga gtgtggctca aattgaggcc aagagagcag aaagagaagc
    50821 aagcaatcct cagttgacac aggtatctgc agatttcata aatgccatgg atctctggaa
    50881 ggattaatcc atgcaagtag gcttgaaatc cgagtaagat ttgcaagctg tgtataattg
    50941 tgatagaata aatagaaaat aaattgaaaa aagaggtatg tgaaatgtca cgtaaaccat
    51001 ttatcgctgg taactggaaa atgaacaaaa atccagaaga agctaaagca ttcgttgaag
    51061 cagttgcatc aaaacttcct tcatcagatc ttgttgaagc aggtatcgct gctccagctc
    51121 ttgatttgac aactgttctt gctgttgcaa aaggctcaaa ccttaaagtt gctgctcaaa
    51181 actgctactt tgaaaatgca ggtgctttca ctggtgaaac tagcccacaa gttttgaaag
    51241 aaatcggtac tgattacgtt gttatcggtc actcagaacg ccgtgactac ttccatgaaa
    51301 ctgacgaaga tatcaacaaa aaagcaaaag caatctttgc gaacggtatg cttccaatca
    51361 tctgttgtgg tgaatcactt gaaacttacg aagctggtaa agctgctgaa ttcgtaggtg
    51421 ctcaagtatc tgctgcattg gctggattga ctgctgaaca agttgctgcc tcagttatcg
    51481 cttatgagcc aatctgggct atcggtactg gtaaatcagc ttcacaagac gatgcacaaa
    51541 aaatgtgtaa agttgttcgt gacgttgtag ctgctgactt tggtcaagaa gtcgcagaca
    51601 aagttcgtgt tcaatacggt ggttctgtta aacctgaaaa tgttgcttca tacatggctt
    51661 gcccagacgt tgacggtgcc cttgtaggtg gtgcgtcact tgaagctgaa agcttcttgg
    51721 ctttgcttga ctttgtaaaa taatcagtaa gtagcaaaag ctaggtggaa cagcattcag
    51781 atgtctgtta cattttttat aggagagaaa gattgaaaac aaaaattgga ttagcaagta
    51841 tctgtttact aggcttggca actagtcatg tcgctgcaaa tgaaactgaa gtagcaaaaa
    51901 cttcgcagga tacaacgaca gcttcaagta gttcagagca aaatcagtct tctaataaaa
    51961 cgcaaacgag cgcagaagta cagactaatg ctgctgccca ctgggatggg gattattatg
    52021 taaaggatga tggttctaaa gctcaaagtg aatggatttt tgacaactac tataaggctt
    52081 ggttttatat taattcagat ggtcgttact cgcagaatga atggcatgga aattactacc
    52141 tgaaatcagg tggatatatg gcccaaaacg agtggatcta tgacagtaat tacaagagtt
    52201 ggttttatct caagtcagat ggggcttatg ctcatcaaga atggcaattg attggaaata
    52261 agtggtacta cttcaagaag tggggttaca tggctaaaag ccaatggcaa ggaagttatt
    52321 tcttgaatgg tcaaggagct atgatgcaaa atgaatggct ctatgatcca gcctattctg
    52381 cttattttta tctaaaatcc gatggaactt atgctaacca agagtggcaa aaagtgggcg
    52441 gcaaatggta ctatttcaag aagtggggct atatggctcg gaatgagtgg caaggcaact
    52501 actatttgac tggaagtggt gctatggcga ctgacgaagt gattatggat ggtgctcgct
    52561 atatctttgc ggcctctggt gagctcaaag aaaagaaaga tttgaatgtc ggctgggttc
    52621 acagagatgg taaacgctat ttctttaata atagagaaga acaagtggga accgaacatg
    52681 ctaagaaaat cattgatatt agtgagcaca atggtcgtat caatgattgg aaaaaggtta
    52741 ttgatgagaa cgaggtggat ggtgtcattg ttcgtctagg ttatagcggt aaagaagaca
    52801 aggaattggc gcataacatt aaggagttaa accgtctggg aattccttat ggtgtctatc
    52861 tctataccta tgctgaaaat gagaccgatg ctgagaatga cgctaaacag accattgaac
    52921 ttataaagaa atacaatatg aacctgtctt accctatcta ttatgatgtt gagaactggg
    52981 aatatgtaaa taagagcaag agagctccaa gtgatacaga cacttgggtt aaaatcatca
    53041 acaagtacat ggacacgatg aagcaggcgg gttatcaaaa tgtgtatgtc tatagctatc
    53101 gtagtttatt acagacgcgt ttaaaacacc cagatatttt aaaacatgta aactgggtag
    53161 cggcctatac gaatgcttta gaatgggaaa acccttatta ttcaggagaa aaaggttggc
    53221 aatatacctc ttctgaatac atgaaaggaa tccaagggcg cgtagatgtc agcgtttggt
    53281 attaagcgat gatttgaaag agggatgtga tagtagcacc ctctttttct ttgttttatg
    53341 atagttcatc ctcgagtaaa ttcaagatct tgctcggaaa tgaagcttat atagtagatt
    53401 gaatatagac aaataccttg tgattggtaa aacattttag aaattcattt acctttccta
    53461 atcgacttgg tttcatctta tttcaatcta ttatagtatt ggggaatttc ttcaaaccac
    53521 atcagcttgg tcagttctac ctgcgacctc aaaacttgtg ctttggtcaa gctgggttta
    53581 gtttcctagt ttgctgatgg atttccattg actataagca tccaaccctc tttttgtctt
    53641 ctaaagaatt cttaaattat cagtctattg caacttttct catataagtt ctttgtcttg
    53701 ctatttgttt tccttagtag tatactaagg tagtaatcat taagaagtgg ttacaaaaaa
    53761 taatgaatga ggtaaagaaa atggtagaat tgaaaaaaga agcagtaaaa gacgtaacat
    53821 cattgacaaa agcagcgcca gtagcattgg caaaaacaaa ggaagtcttg aaccaagctg
    53881 ttgctgattt gtatgtagct cacgttgctt tgcaccaagt gcactggtat atgcatggtc
    53941 gtggtttcct tgtatggcat ccaaaaatgg atgagtacat ggaagctctt gacggtcaat
    54001 tggatgaaat cagcgaacgc ttgattacac tcggtggaag cccattctct acattgacag
    54061 agttccttca aaatagtgaa atcgaagaag aagctggtga ataccgtaat gttgaagaaa
    54121 gcttggaacg tgttcttgtt atctaccgtt acttgtcaga acttttccaa aaaggtttgg
    54181 atgtcactga tgaagaaggt gacgatgtga caaacggtat ctttgcaggc gctaaaactg
    54241 aaacagataa aacaatttgg atgcttgcag ccgaacttgg acaagcacct ggtttgtaag
    54301 aaataaggaa acatagataa atctgtagga aatttcttac agattttttc tattttgtaa
    54361 gctattccaa accagtcttt ttttgagttt tttgataaaa tagtactatc agtgaaaagg
    54421 atggaagcat gactaagaaa atcgtagcta tttgggccca ggatgaagag ggtgtgattg
    54481 gtaaggaaaa tcgtctgcct tggcatttgc cagcagaatt gcagcacttt aaagaaacaa
    54541 ctctgaatca tgctatcttg atggggcgtg tgacctttga tgggatgggg cgtcgcttgc
    54601 ttccaaaacg ggaaaccctg attttgacgc gtaatccgga agaaaagata gatggggttg
    54661 ctacttttca ggacgtccag tctgttcttg actggtatca ggatcaagaa aagaatctct
    54721 acattatcgg tgggaagcaa atttttcagg cttttgaacc ttaccttgat gaagtgattg
    54781 tcactcacat tcatgctcgg gtggaaggag atacctattt ccctgaagag cttgacttgt
    54841 ctctttttga gacagtttca agcaaatttt acgccaaaga tgagaagaat ccttatgatt
    54901 ttaccatcca ataccgcaag agaaaggaag tctaatggaa cggagtttat ttggtctatt
    54961 cacggctttt ctgtgtttca tctgctttct ggcaggagca caggcttttc gtaaaaagcg
    55021 ttacgggctt tctatcctcc tttggttaaa tgcctttacc aatctggtca atagtattca
    55081 tgctttttac atgaccttat tttagataga atgacagtat agaatggaag gaaatcatgt
    55141 ctacaaatag aaaaaatgat atgatggttt attgctcatt ttgtggcaaa aaccaagaag
    55201 aagtacaaaa aataattgct ggcaacaatg cttttatttg taatgaatgc gtggagttag
    55261 ctcaggaaat cattcgagaa gaattggttg aggaagtctt ggcagacttg tctgaggtgc
    55321 caaaaccaat tgaactcctc catatcttga accactatgt aattggtcaa gatcgtgcca
    55381 agcgtgcctt ggcagtggcg gtttataacc actacaaacg catcaatttc cacgatacac
    55441 gcgaagagtc agaagatgtg gatttgcaga agtcaaacat tttgatgatt ggcccaactg
    55501 gttcagggaa aactttcctt gcccagacct tggctaagag cttgaatgtg ccttttgcta
    55561 ttgcggatgc gacagctctg acggaggctg gttatgtggg tgaggacgtg gaaaatatcc
    55621 tcctcaaact cttgcaggtt gctgacttta acatcgaacg tgcagagcgt ggcattatct
    55681 atgtggatga aattgacaag attgccaaga agagtgagaa tgtgtctatc acacgtgatg
    55741 tttctggtga aggggtgcaa caagcccttc tcaagattat tgagggaact gttgctagcg
    55801 tgccgcctca aggtggacgc aaacatccac aacaagagat gattcaagtg gatacaaaaa
    55861 atatcctctt catcgtgggt ggtgcttttg atggtattga agaaattgtc aaacaacgtc
    55921 tgggtgaaaa agtcatcgga tttggtcaaa acaataaggc gattgacgaa aacagctcat
    55981 acatgcaaga aatcatcgct gaagacattc aaaaatttgg tattatccct gagttgattg
    56041 gacgcttgcc tgtttttgcg gctcttgagc aattgaccgt tgatgacttg gttcgcatct
    56101 tgaaagagcc aagaaatgcc ttggtgaaac aataccaaac cttgctttct tatgatgatg
    56161 ttgagttgga atttgacgac gaagcccttc aagagattgc taataaagca atcgaacgga
    56221 agacaggggc gcgtggactt cgctccatca tcgaagaaac catgctagat gtcatgtttg
    56281 aggtgccgag tcaggaaaat gtgaaattgg ttcgcatcac taaagaaact gtcgatggaa
    56341 cggataaacc gatcctagaa acagcctaga ggtgactatg gaacttaata cacacaatgc
    56401 tgaaatcttg ctcagtgcag ctaataagtc ccactatccg caggatgaac tgccagagat
    56461 tgccctagca gggcgttcaa atgttggtaa atccagcttt atcaacacta tgttgaaccg
    56521 taagaatctc gcccgtacat caggaaaacc tggtaaaacc cagctcctga acttttttaa
    56581 cattgatgac aagatgcgct ttgtggatgt gcctggttat ggctatgctc gtgtttctaa
    56641 aaaggaacgt gaaaagtggg ggtgcatgat tgaggagtac ttaacgactc gggaaaatct
    56701 ccgtgcggtt gtcagtctag ttgaccttcg tcatgacccg tcagcagatg atgtgcagat
    56761 gtacgaattt ctcaagtatt atgagattcc agtcatcatt gtggcgacca aggcggacaa
    56821 gattcctcgt ggtaaatgga acaagcatga atcagcaatc aaaaagaaat taaactttga
    56881 cccaagtgac gatttcatcc tcttttcatc tgtcagcaag gcagggatgg atgaggcttg
    56941 ggatgcaatc ttagaaaaat tgtgaggaaa agaaaatggc aaaaacaatt catacagata
    57001 aggccccaaa ggctatcgga ccctatgttc aaggaaaaat cgttggcaac cttttgtttg
    57061 ctagcggtca agttccccta tcccctgaaa ctggggaaat tgtaggagag aatatccaag
    57121 aacagacaga gcaagtcttg aaaaacatcg gtgctatttt ggcagaagca ggaacagact
    57181 ttgaccatgt tgtcaaaaca acttgcttct tgagcgatat gaacgacttt gttcctttta
    57241 atgaggttta ccaaacggcc ttcaaagagg aattcccagc tcgttcagct gtggaggtag
    57301 ctcgtcttcc tcgtgatgta aaagtcgaaa ttgaagtcat cgcagagatt ggataagcta
    57361 gttgaagttt ggtgttgcca aacttctttt gatataagga gaaaaagatg acaaagaaac
    57421 aacttcactt ggtgattgtg acagggatga gtggcgcagg gaaaactgta gccattcagt
    57481 ccttcgagga tctaggttat ttcaccattg ataatatgcc gccagctctc ttgcctaagt
    57541 ttttgcagct ggttgaaatt aaggaagaca atcctaagtt ggccttggta gtggatatgc
    57601 gtagccgttc tttcttttca gagattcaag ctgttttgga tgagttggaa aatcaagatg
    57661 gtttggattt caaaatcctc tttttggatg cggctgataa ggaattggtc gctcgttaca
    57721 aggaaaccag acggagtcac ccactagcag cagacggtcg tattttagat ggaatcaagt
    57781 tggaacgtga actcttggca cctttgaaaa atatgagcca aaatgtggtg gatacgactg
    57841 aactcactcc acgtgagctg cgcaaaaccc ttgcagagca gttttcagac caagaacaag
    57901 cccagtcttt ccgtatcgaa gtcatgtctt tcggatttaa gtatggaatc ccgattgatg
    57961 cggacttggt ctttgatgtc cgtttcttgc caaatcccta ttatctacca gaactgagaa
    58021 accaaacggg tgtggatgaa cctgtttatg attatgtcat gaaccatcct gagtcagaag
    58081 acttttatca acatttattg gccttgattg agccgattct gccaagttac caaaaggaag
    58141 gtaagtccgt tttgaccatt gccatgggat gtacgggtgg acaacaccgt agtgtggcat
    58201 ttgctaaacg cttggcgcag gacttatcca agaattggtc tgttaatgaa gggcatcgcg
    58261 acaaagaccg cagaaaggaa acggtaaacc gttcatgaga aaaccaaaga taacggtgat
    58321 tggtggaggg actggaattc ccgtcattct aaaaagtctg cgggaaaaag atgtggaaat
    58381 cgcagctatc gtgacggtgg cagatgatgg tggttcttca ggtgaactcc gaaaaaatat
    58441 gcaacagttg acaccgccag gtgatcttcg taatgtcctt gtggccatgt cggatatgcc
    58501 taagttttat gagaaggtct ttcagtatcg gttctctgag gatgccggag cctttgctgg
    58561 ccatccattg ggaaatctca tcattgctgg cttgtcagaa atgcagggtt caacctataa
    58621 tgccatgcag ttattgagca aatttttcca tacaacaggg aaaattcatc cttccagtga
    58681 ccatcctttg acccttcatg cagtctttca ggatgggaca gaagtggctg gagagagtca
    58741 tattgtagac catcgaggca taattgacaa tgtctatgtg accaatgccc taaacgatga
    58801 tacgcctctg gccagccgtc gagtagtgca gaccatcctt gaaagtgaca tgattgtcct
    58861 agggccaggt tccctcttta cctctatttt gcccaatatc gtgattaagg aaattgggcg
    58921 ggctcttttg gaaaccaagg cagaaattgc ctatgtctgc aatatcatga cccaacgtgg
    58981 ggagacggaa cactttacag atagcgacca cgtggaagtc ttgcatcgtc accttggtcg
    59041 cccttttatc gacactgtct tggtgaatat tgaaaaagtg cctcaggaat acatgaattc
    59101 caaccgtttt gatgaatact tagtgcaagt ggaacacgat tttgtaggtc tttgtaagca
    59161 agtttcgcgc gtgatttcat ctaacttcct tcgtctggaa aatggcggtg ccttccacga
    59221 tggagatttg attgtggacg agttgatgcg cattatacag gtgaaaaaat gagtttcaca
    59281 gtagcagtaa aagaagaaat cctgggccaa caccatctga gctggcatga attatctgcc
    59341 attatcaaaa tgtctggtag catcggtctc tcgacttcgg gcttgacttt gtcagttgtg
    59401 acagaaaatg ccaaactggc ccgtcacctc tatgagtcct ttctccattt ctatgaaatc
    59461 aaatctgaaa ttcgtcacca ccaacggagc aatcttcgca agaatcgcgt ctataccgtt
    59521 tttacagatg aaaaggtgca ggatttgtta agtgatttgc acttggcaga ctctttcttt
    59581 ggcctggaaa caggtattga tgaggcgatt ttatcggatg aggaagcagg tcgtgcctat
    59641 ctctgtggcg ctttcttggc aaatgggagc attcgtgacc ctgagtcagg caagtaccag
    59701 ttggaaatca gttctgttta tctggaccac gcgcaaggaa ttgcctccct tctccaacag
    59761 tttttactgg atgccaaggt gcttgagcgc aagaaggggg ctgtgaccta tctccagcga
    59821 gcagaagaca ttatggactt cttgatagta ataggagcca tgcaggcacg tgatgatttt
    59881 gagcgggtta agattttgcg agaaacccgt aacgacctca atcgggctaa taatgccgag
    59941 acagctaata tcgctcggac agtttctgcc agcatgaaga ccattaacaa tatcagtaaa
    60001 atcaaagata tcatgggctt agaaaatttg ccagtagatt tgcaggaagt ggcgcaactg
    60061 aggattcagc acccagacta ctctatccag cagttagcag atagtctcag cacccctctg
    60121 accaaaagtg gtgtcaatca cagactcaga aaaatcaata agatagcgga tgaattataa
    60181 aaccacgaat cctatgtgac tcgtggttct tttttataaa ctggtagagt gttttggttg
    60241 tactttttgt tcagggtcaa tgtagttgat agcgttgttg acagcagttg gagcttctcc
    60301 gaggcctgtc gcaatcagat caatttttcc gtcatagtag cagcagtcac cgatagcata
    60361 gatacctgct tggctggatt cctgtttgct gttgacgata atcttgtgac ggttgaggtc
    60421 gagcccccag tttttaaggt taccgacaga agatttgaaa ccatagttga caaagaggtg
    60481 gtctagatca atggtttcag tttcatcaga tttgactttt gtgatttcaa gtttatcaag
    60541 tgtttttcca tttccaagga gttggctagg ggcgaatggt gtcttgatgg ttacagatga
    60601 ttcttgcaag gcttgaacac tgtgttccaa ggcacggaaa ttatctctgc ggtgaacaag
    60661 ggtagttggt gcgatttttt caaaagccaa agcccaatcc acagccgagt ctcccccacc
    60721 aagaatcgtc actttcttac cagcgtattg ctgaatgtta gaaacgtggt agtggatatt
    60781 ttcatagccc tcaacccctt caagttccag cggacgtggt ttgaaggcac cgccacccat
    60841 agcgatgata actgttttag tcaggtgact tcctttagaa gttgtgatgg caaattcttc
    60901 ttgtttgtca atctcaagaa ccgtttcatt gagatgaata ggggtatcaa atccatttag
    60961 ctgttcaatc aagcggttag tcaactcttc tccagtcagg tttgggaagc ctggtacgtc
    61021 taggatttcc ttttcagggt agagaatagc aggttgtcca cctagctggg gaagagagtc
    61081 gatgatttga accttggctt ggcgtaggtg ggcataaaag gctgcaaaaa gcccgacagg
    61141 accaccaccc acaatggtaa tatcatagag ttgagacata gtttctcctt tgttttttct
    61201 agtcagttta ttttatcata tttttgttca atgtaaaatg gactaggata gggaaaaaaa
    61261 tgccagaaaa tggtataata ggacgaaagt gttttgatag ggaggatatc agggatgaat
    61321 tttcaacaat tatccaatct gcaatattgg accagtttgt ttgcaagtcc atggacgata
    61381 gctatcaatc tgattgatat tttgattgtt gcttatattt tataccattt tacaaaagct
    61441 attgcaggaa ccaagattat gattttggta cgtggagttt tggtgtttat tttagctcaa
    61501 atccttgcaa atatgattgg tttgactacg atttcttggt taatcaatca aattattact
    61561 tatggggtta ttgcggcggt tgttatcttc tctccagaga ttcggactgg tttggaacgt
    61621 ttgggaagag cgacagattt cttttccaat gcccctatta gtgctgagga acagatgatt
    61681 cgtgcctttg ttaagtctgt tgaatacatg agtcctcgta aaatcggggc cttggttgct
    61741 attcagcgtg tacgtacctt gcaggagtat acttcgacag gaattccctt ggatgctaag
    61801 atttctgcag aacttctcat taacattttt attcccaaca ctcccctaca tgatggtgcg
    61861 gtgattatca aagaagaacg tatcgctgtg acgtctgcct atctgccctt gacaaaaaac
    61921 acagggattt ccaaggaatt tgggaccaga caccgggcgg ctatcggttt atcagaagtc
    61981 tcagatgcct tgacttttgt cgtatcagag gaaacgggag gaatttcgat aacctataat
    62041 ggaaggttta agcacaacct aacacttgat gaatttgaaa cagaattacg tgaaatctta
    62101 cttccaaaag aggaagtggg tcttagtttt aaagaacgat tgctaggagg atggaaacat
    62161 gaaaaaaaat agtttatata tcatatcctc actctttttt gcttgtgtct tatttgtcta
    62221 tgctacggcg acgaattttc aaaacagtac cagtgctagg caggtaaaaa cggaaaccta
    62281 tactaataca gtaacaaatg tccctattga catacgctat aatagtgata agtattttat
    62341 tagcggtttt gcttcagaag tatcagtggt cttgactggt gcaaatcgcc tatcgctagc
    62401 tagtgaaatg caagaaagta cacgtaaatt caaggttact gctgacctaa cagatgccgg
    62461 tgttggaacg attgaagttc ctttgagcat tgaagattta cccaatgggc tgaccgctgt
    62521 ggcgactccg caaaaaatta cagtcaagat tggtaagaag gctcagaagg ataaggtaaa
    62581 gattgtacca gagattgacc ctagtcaaat tgatagtcgg gtacaaattg aaaatgtcat
    62641 ggtgtcagat aaagaagtgt ctattacgag tgaccaagag acattggata gaattgataa
    62701 gattatcgct gtcttgccaa ctagcgaacg cataacaggt aattacagtg gttcagtacc
    62761 tttgcaggca atcgatcgca atggtgttgt cttaccggca gttatcactc cgtttgatac
    62821 aataatgaag gtgactacaa aaccagtagc accaagttca agcacatcaa attcaagtac
    62881 aagcagttca tcggagacat cttcgtcaac gaaagcaact agttcaaaaa cgaattaaaa
    62941 atagaaaagg attttataac aatgggtaaa tattttggga ctgatggagt ccgtggagaa
    63001 gctaacctag aactaacacc agaattagcc tttaaactag gacgttttgg aggctatgtt
    63061 cttagtcaac atgaaacgga agcgccgaaa gtctttgtag gacgtgacac acgtatttca
    63121 ggggaaatgc tggaatcggc cttggtggca ggtctccttt cagtagggat tcacgtatac
    63181 aaacttggtg tccttgcaac accagcagta gcttacttgg ttgaaactga aggagcaagt
    63241 gccggtgtca tgatttctgc tagccacaac ccagcccttg ataacggaat caagttcttt
    63301 ggcggtgatg gcttcaaact agatgatgaa aaagaagcag aaattgaagc cttgctagat
    63361 gctgaggaag acactcttcc tcgtccaagt gcagaaggct taggaatttt ggtagattat
    63421 ccagaaggct tgcgtaagta tgaaggatac cttgtttcaa ctggaactcc tcttgatgga
    63481 atgaaggttg ccttggatac agctaatgga gcagcttcta ccagtgcccg tcaaatcttt
    63541 gcagaccttg gtgcccaatt gacggttatc ggggaaacac cagacggtct taacatcaac
    63601 cttaatgttg gttcaacaca tccagaagcc cttcaagaag tggtcaaaga aagtgggtca
    63661 gctattggtt tggcctttga tggagacagt gaccgcttga ttgctgttga tgagaatggt
    63721 gacatcgtcg atggtgacaa gattatgtac atcatcggaa aatacctttc tgaaaaagga
    63781 caattgactc aaaatacaat tgtgacaact gttatgtcta accttggttt ccacaaggcc
    63841 ttgaatcgcg aaggtattaa caaggcagtt actgcagttg gtgaccgcta cgttgttgaa
    63901 gaaatgagaa aatcaggtta caaccttggt ggtgaacagt ctggtcacgt tatcttgatg
    63961 gattacaata ccacaggtga tggtcaatta tcagcagttc aattgactaa aatcatgaag
    64021 gaaactggta agagcttatc agagttggcg gcagaagtaa cgatttatcc acaaaaatta
    64081 gttaatatcc gagtggaaaa cgtcatgaag gaaaaggcca tggaagtgcc agctatcaag
    64141 gccatcatcg agaagatgga agaagaaatg gcggggaacg gccgtatcct tgttcgtcca
    64201 agtggaacag aacccctctt gcgtgttatg gcagaagcgc ctacaacaga agaagtagac
    64261 tactatgttg ataccatcac agatgtagtt cgtgctgaaa ttgggattga ctaaatccta
    64321 gaaaactaga tgaaaataaa aaattatggt acaatagctt ataatattgt agccgatgga
    64381 gaaagacatg aatcttaaac gtgaacaaga atttgttagt cagtatcatt ttgatgcgcg
    64441 taattttgaa tgggaaaatg aaaatggagc tcctgaaacc aaggtggatg tgaatttcca
    64501 attgctccaa catgaccaag aaaatcaagt gacttccttg attgtcatct tgagctttat
    64561 gattgtattt gataagtttg tcattagtgg aacaatctca caagtaaatc atatcgatgg
    64621 ccgtattgtc aatgaaccaa gtgaattgaa ccaagaagag gtggaaacct tggctcgtcc
    64681 atgtttgaac atgctcaatc gtttgactta cgaagtgact gaaattgcat tagacttacc
    64741 aggaattaat ttggagttct aatatgaaat tagctgtttt cacagattcc tctgcttatc
    64801 tcagtgcaga gatcttgcaa agagaagact tgtttgtctt ggatattcct gtcaatattg
    64861 atggtgagga atatgtcgaa ggcatcaatc tgtctgctga ggaattttac caaaaaatgg
    64921 ctcaggcttc tgaattgcct aagaccagtc aaccaagtat tgccaagtta gatgaaatct
    64981 taacttcgct caaagaacaa ggctatacac atgccttggg gcttttccta tcttctggaa
    65041 tttcaggttt ttaccaaaat atccagtata tggtcgatga ctatgagggc ttaaccattg
    65101 ctttcccaga cactttgatt acaagtgctc ccctaggcat catggttgaa aacgtcttta
    65161 attggcgtga tcagggcgat gattttgcca gcattcagga taagctagcc attcaaatca
    65221 gccgtacgtc agcctttatc atggtagatg acttggatca tttggtaaaa ggtggacgcc
    65281 tttcaaatgg ggctgccatt ttgggcaatt tgcttagcat taagccaatc ctttatttta
    65341 acgatcaagg tgtgattgaa gtttacgaaa aagttcgtac tgaaaagaag gccaccaagc
    65401 gcttaattga aattatcaag gaaacaacgg cttcaggtca ataccgggtc attgtcattc
    65461 atgggaatgc tcctgaaaag gctgaagaat tgcgtcagca cttgcttgat tttggcttgg
    65521 gttcggatgt ttcacttgct acatttggta gtgtcattgg aacgcaccta ggagcaggaa
    65581 gtattgctct gggttatatt ccagtgattt agttggcgct tctaggctag ttaagatgcc
    65641 ttgtatctta gaaattgaac acggactacc tgcctcttga aaaaagatgc ctgtcttgcc
    65701 tttcgtggaa agtcagtgcc attccctatt tttcagaggc agctaaggtc ctttgtatct
    65761 tgataattga acacgcctgg aaccctgtgt gaaaaagata gttcttccaa gaagtagaca
    65821 cttcttgatt ggaattccta ttttcacttt gtgttcttac ggactttgta tcttagacag
    65881 gagtagagat gagtattcga gtaattattg ccggttttaa gggaaagatg ggccaggctg
    65941 cttgtcagat ggtattgact gatccagact tggacttggt agcagttttt gatccttttg
    66001 agtctgagtc agaatggcag ggtattcctg ttttcaagga taaggctgat ttagctggtt
    66061 ttgaagcgga tgtctgggta gattttacta ctccagctgt tgcctacgaa aatacacgtt
    66121 ttgctcttga aaatggcttt gctccagtag ttggaacgac tggtttcacg agtgaagaaa
    66181 ttgcagagtt aaaagaattt tctcgtgccc aagacttggg tggcctgatt gcccctaact
    66241 ttgccttggg tgctgtctta ctcatgcaat ttgcgacgca ggctgccaaa tatttcccaa
    66301 atgtggagat tattgagctc catcatgaca agaaaaagga tgctccgagt ggaacagcca
    66361 ttaaaacagc tgagttgatg gcagaggttc gagagtccat tcagcaaggc gcagcagatg
    66421 aggaagagct gattgctggt gctcgtggtg ctgactttga tggtatgcgc atccactcag
    66481 ttcgtttgcc aggcttggta gctcatcaag aagtcatctt tggcaatcag ggagaagggt
    66541 tgaccctccg tcatgactcc tatgatcgca tctccttcat gacaggagtc aatttgggaa
    66601 ttaaagaagt tgtcaagcgt catgagcttg tctatggatt agaacactta ttatgagatt
    66661 aacgcaaatg ccttctgaat ttcagaaggc tttaccagta ttagaaaaaa ttaaagaagc
    66721 aggctttgag gcttattttg ttgggggctc tgttcgagat gcccttctcc atagccctat
    66781 ccacgatgtg gatattgcga cgtcttctta tccagaagag accaagcaga tttttccgcg
    66841 aacagccgat atcggaatcg agcatggaac cgtcttggtc ttagatgggg atgaggagta
    66901 tgaggtaaca acctttcgga cagaggatgt ctatgtggac tatcgcagac ccagtgcggt
    66961 ttcctttgtg cgctcgctag aagaagacct caaacgccgt gatttcacag tcaacgcctt
    67021 tgccttggat gagacaggag aaatcgttga cttgttccat ggtttagaag atttggaaaa
    67081 gcaagtcttg cgagcagttg gagtggctag tgagcgtttc aacgaagatg ctttacggat
    67141 tatgcgtggt ttccgttttc aggctagtct tggttttgca cttgagccag aaacatttaa
    67201 agctatgaag accttgacgc cgcttttgga gaaaatttct gtagagcgta ccttcgtcga
    67261 gtttgataaa ctcttgctgg ctccattttg gagaaggggc ttggcttcca tgattgagag
    67321 tcaagcttat gactatctcc ctgatatggc atctagccag aacaagctca acagactgtt
    67381 tgatttggag actgatttta cttttgaatc ctctgaacaa gcctgggcgg ctttactatg
    67441 ggctttggag attgaaaatg cgcagtcatt tttgaaatct tggaagacct cacgccagtt
    67501 tgccaagcaa gttcaggatt tgctgattat tttggctctg cgtgaaaatg gagaattgag
    67561 caagcgagat tgttatcgct ttgacataga tttgctttta caggctgaaa atcttcgtca
    67621 agctcgagga aaagaagtca acccacaagc catcacagaa aaataccaaa gcttgactat
    67681 ccatgacaag aaagagattc agattaatgg cgatattttg atcaaggaat atggctatca
    67741 gccaggccca gacttgggag agattttaac agagattgag tttgccattg tcgatggaga
    67801 attggagaat aatcgtgaag ccatccatgc ttacctgagg gagaaaaaat gagtgatttt
    67861 atcgttgaaa aactaagtaa atccgttggt gacaagaccg tttttaggga tatttccttt
    67921 attatccatg acttagacag aattggttta atcggtgtca atgggactgg caagaccacc
    67981 cttttggacg tcctttctgg tgtttctggc tttgatgggg atgtcagtcc tttttcagct
    68041 aaaaatgatt accagattgg ttacttgact caggatcctg attttgatga tagaaagaca
    68101 gttttggata cggttctatc tagtgaactc aaggaaatcc agctcattcg tgagtatgaa
    68161 ttgattatgc tcgactatag tgaggacaag caggcgcgtt tggaacgtgt catggcagag
    68221 atggactctc tccaagcttg ggaaattgag agtcaggtca agaccgttct tagcaaattg
    68281 ggcattcaag acttatctac tcctgttggg gaattgtcag gtggtctgag aagacgggta
    68341 cagttggcac aagtcttact tggcaaccac gacctcttgc ttttggatga gccgaccaac
    68401 catctggata ttgcgattat tgagtggctg accctctttt tgaaaaattc taagaagacc
    68461 gtccttttta tcactcacga tcgttatttc ttagacgctt tgtcaacacg gattttcgag
    68521 ttggagcgag caggcttgac agaatatcag ggcaattacc aggactatgt tcgccttaag
    68581 gcagaacagg acgagcgcga tgcagctcta cttcataaaa aagaacaact ctacaaacaa
    68641 gaactggcct ggatgcgcag acaaccgcag gcacgtgcga ccaagcaaca agctcgtatc
    68701 aatcgtttcc atgatctgaa aagggaagtt tcaggcagta gtgctgagac agacttgact
    68761 atgaactttg aaaccagtcg gattgggaag aaagtcatcg agtttcagga tgtttccttt
    68821 gcctatgaaa ataagcccat tttgcaagat tttaatctct tggtgcaggc caaagaccgt
    68881 atcggaatcg ttggggacaa tggtgttgga aaatcaaccc tacttaacct gattgcagga
    68941 agtcttgagc cgacagcagg acaagttgtg attggggaaa ctgttcgcat cgcctatttc
    69001 tctcagcaaa ttgagggttt ggatgaaagc aagagagtta tcaattactt gcaggaagtg
    69061 gcagaagagg tcaagaccag tggtggttct acgacttcca tcgctgagtt gctggagcaa
    69121 ttcctcttcc cacgttcgac gcatgggacc ttgattgaga aattgtcagg gggtgagaaa
    69181 aaacgtcttt atctcctcaa actgcttttg gaaaaaccaa atgttcttct tttagacgag
    69241 ccaaccaatg acctagatat tgcaactttg acagtcttag agaatttctt gcaaggtttt
    69301 gcaggtcccg ttttaacagt cagtcacgac cgctatttct tggataaggt agcgaccaag
    69361 attctcgctt ttgaggatgg caagattcgt cctttctttg gtcattacac cgactatctt
    69421 gatgaaaaag cttttgaaac agatatggcc aatcaagtgc aaaaggccga aaaggaaaaa
    69481 gtggtcaagg ttcgagaaga caagaaacgc atgacctacc aagaaaagca ggagtgggca
    69541 agtattgaag gtgatattga aaccttggaa aaacgtatcg ctgctattga agaggaaatg
    69601 caggctaacg gctctgactt tggtaagctg gctactctcc aaaaagaatt ggatgagaaa
    69661 aatgaagcac tccttgaaaa atacgaacgc tatgagtatc tcagtgaatt tgatagttaa
    69721 gactattcga aaatcaaagt gcaaactatg tcggcttcac attgccgact caagtacagc
    69781 ctgtggccgg cttcctagtt tgcactttga cttttattga gtataaaaga atagaaaaat
    69841 cccatctcat gaacggattt ctctattctt tttttgtttc ttgatgaaag atattttgcc
    69901 aggtttcgtg gcgacgccag atactggtat ggatgatacc atctaactca tagcagatga
    69961 gtttggtttt ttgactgatg gaagtaattt gaatgtcctt gatagcagaa tttaactctt
    70021 ttttggtctt ataagtctct ttatccatct gctctccatc ttggcgaata tagacaaaat
    70081 catcagtcaa gagttcttct agttggtttc cttggtcaat caattgttca cgcataagca
    70141 attttttata gacattgtct aaaatttcat cctcagggat aggtgctggt tcgatatgga
    70201 catcggtatc aaagacgcca aaacgatcct ccagcataga ctcgacctga tccgcgattt
    70261 catggctttc aaaaacagac aagtcaggat tcatctctag tgtaatatcc aggtagatgt
    70321 tgctaccgta ggtgcgacct ctttgcgatt tgaccttgct gattttggga atttccatga
    70381 tagccttttg gtagtcctcg agcaggcggt cgtcaaagcc atctgaaaga ctaaaggaag
    70441 actcgatgaa gatatcatag gcagtcttca agataaagaa agtgatgatg atagcaacca
    70501 gtttatccac aatcggataa ttgaaactac tagctaggat ggcaatggcg gttccaagtg
    70561 aggtaacagc gtcagaaaga ttgtccttag cagctgcctt cagcgccttg gagttggatt
    70621 tcttactgag gcgagtattg tagagataaa ctagaaacat aatcgctgca gatataattc
    70681 ctagagttgc acccagagga tcaatgaccg tttcttcacg actgagaatc ttctgaatgg
    70741 tatccctcag cacatcaaag ccaacataaa acatgatgat ggaagtgatt aagctagcca
    70801 aatcttcaat cttccaatgg ccaaaacggt ggtcccggtc tgcaggttgg cgcgccattc
    70861 gaatgccaat caagagggcc acatttccaa tgatgtccga gatgttgtta aaaccatcgg
    70921 ccaccaaact ggatgaatga agaagatggc cagtagccaa ttttgctgct gataagagta
    70981 gataggtaga aatactgata atggccccac gctcagctaa cttgagattt gagatagatt
    71041 gcttcattca acttcctttc tagtcacata gcattctacc attatactgg ttttcaagag
    71101 aaaattcaaa taactaagaa gagattagaa attgatctca taaa
//
LOCUS       DAVKWZ010000005        57934 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000005 DAVKWZ010000000
VERSION     DAVKWZ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 57934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57934
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.005"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(144..1106)
                     /locus_tag="VW854_000325"
     CDS             complement(144..1106)
                     /locus_tag="VW854_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEV0261623.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKNYMEG"
     gene            1172..2560
                     /locus_tag="VW854_000326"
     CDS             1172..2560
                     /locus_tag="VW854_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV0261624.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRVGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAADKVAKKTPAQQDLIGQVDALIDMLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            complement(2595..3047)
                     /locus_tag="VW854_000327"
     CDS             complement(2595..3047)
                     /locus_tag="VW854_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV0261625.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            3209..3503
                     /locus_tag="VW854_000328"
                     /pseudo
     CDS             3209..3503
                     /locus_tag="VW854_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3659..4873
                     /locus_tag="VW854_000329"
     CDS             3659..4873
                     /locus_tag="VW854_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV0261626.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            5018..5152
                     /gene="rpmH"
                     /locus_tag="VW854_000330"
     CDS             5018..5152
                     /gene="rpmH"
                     /locus_tag="VW854_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEV0261627.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            5436..6317
                     /locus_tag="VW854_000331"
     CDS             5436..6317
                     /locus_tag="VW854_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEV0261628.1"
                     /translation="MVAQDPKAQDSTKPTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVAGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATPEY
                     KLENTPGGDKGGNTGSSDANANAGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKDKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGV
                     QAIVMVTVPKRPVAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALASLGLV
                     VATSGFALLGRKTRRRK"
     gene            6622..7395
                     /locus_tag="VW854_000332"
     CDS             6622..7395
                     /locus_tag="VW854_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEV0261629.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVTQMNIVGFDKPTIE
                     RALELVDEYDQLYATIGWHPTEAGTYTEEVEAYLLDKLKHPKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKILVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            7395..7955
                     /gene="rnmV"
                     /locus_tag="VW854_000333"
     CDS             7395..7955
                     /gene="rnmV"
                     /locus_tag="VW854_000333"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEV0261630.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINAQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVRHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            8027..8926
                     /locus_tag="VW854_000334"
     CDS             8027..8926
                     /locus_tag="VW854_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0261631.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLDYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMPQIIEKEAYSINDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTQIENYFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALRVERDFSLAESFYQSSKTFAK
                     LIGDGFLVKKLTEEWQEDVKKYL"
     gene            9246..9542
                     /locus_tag="VW854_000335"
     CDS             9246..9542
                     /locus_tag="VW854_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEV0261632.1"
                     /translation="MKQTVKKLALVASIAATLGGGVSVASAAVQYPEGGVWTYGSGNG
                     GAYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
     gene            9619..11649
                     /locus_tag="VW854_000336"
     CDS             9619..11649
                     /locus_tag="VW854_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV0261633.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETRFTYATYGEGKLPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSIYYQGLFTDYL
                     QNFSSESVEVTSQKHYLPQVRLAFTEIGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRDLMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            11651..12292
                     /locus_tag="VW854_000337"
     CDS             11651..12292
                     /locus_tag="VW854_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0261634.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            12285..12443
                     /locus_tag="VW854_000338"
     CDS             12285..12443
                     /locus_tag="VW854_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261635.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQILVHGYL
                     LDFASIEG"
     gene            12829..13701
                     /gene="rsmA"
                     /locus_tag="VW854_000339"
     CDS             12829..13701
                     /gene="rsmA"
                     /locus_tag="VW854_000339"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEV0261636.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPDLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            13703..14581
                     /gene="rsgA"
                     /locus_tag="VW854_000340"
     CDS             13703..14581
                     /gene="rsgA"
                     /locus_tag="VW854_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEV0261637.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKLVPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            14592..15248
                     /locus_tag="VW854_000341"
     CDS             14592..15248
                     /locus_tag="VW854_000341"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEV0261638.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTPVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGEVNERVQTLRKQLD
                     "
     gene            15211..15873
                     /locus_tag="VW854_000342"
     CDS             15211..15873
                     /locus_tag="VW854_000342"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEV0261639.1"
                     /translation="MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLE
                     EDLPLALAVGDFDSVTEEERQVIQKRAQHFVQARPEKDDTDLELALLTIFKQNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMHQIEIEDGQNLITYCPEGISQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            15875..17131
                     /locus_tag="VW854_000343"
     CDS             15875..17131
                     /locus_tag="VW854_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEV0261640.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVAVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYEVGDKDEIERCRKSLLASVKRFARDIRNKYI
                     APPRTTNFGVLFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            17121..18062
                     /locus_tag="VW854_000344"
     CDS             17121..18062
                     /locus_tag="VW854_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEV0261641.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEIYSQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMSNKIFAMDNRSFYKPDLD"
     gene            18329..19156
                     /gene="purR"
                     /locus_tag="VW854_000345"
     CDS             18329..19156
                     /gene="purR"
                     /locus_tag="VW854_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEV0261642.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            19223..20473
                     /locus_tag="VW854_000346"
     CDS             19223..20473
                     /locus_tag="VW854_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEV0261643.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERAAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     IWLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            20599..21393
                     /gene="pflA"
                     /locus_tag="VW854_000347"
     CDS             20599..21393
                     /gene="pflA"
                     /locus_tag="VW854_000347"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEV0261644.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTRQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(21468..22394)
                     /gene="yidC"
                     /locus_tag="VW854_000348"
     CDS             complement(21468..22394)
                     /gene="yidC"
                     /locus_tag="VW854_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV0261645.1"
                     /translation="MKSIKRFAFSAMGVAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(22472..22750)
                     /locus_tag="VW854_000349"
     CDS             complement(22472..22750)
                     /locus_tag="VW854_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEV0261646.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            22784..23524
                     /locus_tag="VW854_000350"
     CDS             22784..23524
                     /locus_tag="VW854_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEV0261647.1"
                     /translation="MTIITSKANSVVKNAKKLRQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRDQLATFSQTVWVSEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVATGILIFHLS"
     gene            23580..24263
                     /locus_tag="VW854_000351"
     CDS             23580..24263
                     /locus_tag="VW854_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEV0261648.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYLLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            24376..25368
                     /locus_tag="VW854_000352"
     CDS             24376..25368
                     /locus_tag="VW854_000352"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEV0261649.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            25433..25972
                     /gene="rsmD"
                     /locus_tag="VW854_000353"
     CDS             25433..25972
                     /gene="rsmD"
                     /locus_tag="VW854_000353"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEV0261650.1"
                     /translation="MKIVSGIYGRRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYTKEQIVADIEKMAERELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            25962..26450
                     /gene="coaD"
                     /locus_tag="VW854_000354"
     CDS             25962..26450
                     /gene="coaD"
                     /locus_tag="VW854_000354"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEV0261651.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPIENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATCLVRGLRNASDLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACYVPESILEEIRNEK
                     KD"
     gene            26434..27471
                     /locus_tag="VW854_000355"
     CDS             26434..27471
                     /locus_tag="VW854_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEV0261652.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHSDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            27529..27759
                     /locus_tag="VW854_000356"
     CDS             27529..27759
                     /locus_tag="VW854_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEV0261653.1"
                     /translation="MVQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTDENTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            27783..29066
                     /gene="murA"
                     /locus_tag="VW854_000357"
     CDS             27783..29066
                     /gene="murA"
                     /locus_tag="VW854_000357"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV0261654.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            29059..29247
                     /locus_tag="VW854_000358"
     CDS             29059..29247
                     /locus_tag="VW854_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV0261655.1"
                     /translation="MNKKSSYVINRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            29286..30110
                     /gene="endA"
                     /locus_tag="VW854_000359"
     CDS             29286..30110
                     /gene="endA"
                     /locus_tag="VW854_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEV0261656.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTNLSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            30401..31732
                     /locus_tag="VW854_000360"
     CDS             30401..31732
                     /locus_tag="VW854_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEV0261657.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPIIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(31930..32000)
                     /locus_tag="VW854_000361"
     tRNA            complement(31930..32000)
                     /locus_tag="VW854_000361"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31966..31968),aa:Cys,seq:gca)
     gene            32416..36027
                     /gene="rpoB"
                     /locus_tag="VW854_000362"
     CDS             32416..36027
                     /gene="rpoB"
                     /locus_tag="VW854_000362"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV0261658.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVIAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            36060..39737
                     /gene="rpoC"
                     /locus_tag="VW854_000363"
     CDS             36060..39737
                     /gene="rpoC"
                     /locus_tag="VW854_000363"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEV0261659.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGAIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            39848..40261
                     /gene="ndk"
                     /locus_tag="VW854_000364"
     CDS             39848..40261
                     /gene="ndk"
                     /locus_tag="VW854_000364"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEV0261660.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEIIQNVVHGSDSEESAKREIALWF"
     gene            complement(40771..41049)
                     /locus_tag="VW854_000365"
     CDS             complement(40771..41049)
                     /locus_tag="VW854_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComA"
                     /protein_id="HEV0261661.1"
                     /translation="MKKFDNYIIEKPCDSNSDKLQKILIIENLVDDILQFPLRINNSV
                     GEIFLLQPFQKKTIFIPCYFEEDIVKVKDDDKVEWNLLEFQKFRAFLA"
     gene            complement(41190..41822)
                     /locus_tag="VW854_000366"
     CDS             complement(41190..41822)
                     /locus_tag="VW854_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0261662.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLAYLNLDKRIFELSGGESQRVALAKVILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDRLK"
     gene            complement(41824..43839)
                     /locus_tag="VW854_000367"
     CDS             complement(41824..43839)
                     /locus_tag="VW854_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV0261663.1"
                     /translation="MKKLFILISNLLASLFFVWVFTIWTDTYVSHYYPNVVVRDSSPE
                     TTFQHVATRLEKLAEETDSFIAIQHQDPNSEGTTVFSYTTFGDGKLPDGLQEKNLEDA
                     QSSSVETNYFVFDGHLDIHLLREELSQLGLTNMHLTIPSKLSTLMAIFSNGFQLISLL
                     IFILTFVALTLISQISQLRSSGIRLISGEKRWSIFLRPVGEDLKGIAVGFSLAGVLAI
                     LMQKILSLPTQSLMTIGAGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVR
                     GIISLILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEVWSQERQLITLSISREGTS
                     PGFDEQAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNLTTSTEWHDY
                     SPNGNVLIVTPHYLERQNIPVDTTIKQKMNHLNVGEFVLLLPEHLRSEEEHYKSVFED
                     DLTSRMSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQ
                     SILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVM
                     LAGAVLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLG
                     FVASVFLQVEIGVAFLVLLLFTGLSLLQLHVQMQKENKMSMLVLKGG"
     gene            complement(43942..44238)
                     /locus_tag="VW854_000368"
     CDS             complement(43942..44238)
                     /locus_tag="VW854_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEV0261664.1"
                     /translation="MLKFRKVVASSLAVLAFSLALPVLAVSHRGGEWTYGGHHDPNNW
                     GAFSNYYHGSQYHWAYVGSNGRNNQKTVYAGARSAAYAFVNTNFGEQVTFDAGW"
     gene            44898..45761
                     /locus_tag="VW854_000369"
     CDS             44898..45761
                     /locus_tag="VW854_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0261665.1"
                     /translation="MNTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEIKSNLSNFKQLSARLLDDRNYENLEYIYRIEIERS
                     TFLTLEDRTYLEWIKSIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELDTV"
     gene            46144..46764
                     /locus_tag="VW854_000370"
     CDS             46144..46764
                     /locus_tag="VW854_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261666.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            46850..47293
                     /gene="tsaE"
                     /locus_tag="VW854_000371"
     CDS             46850..47293
                     /gene="tsaE"
                     /locus_tag="VW854_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEV0261667.1"
                     /translation="MYIKNEEELQALGERLGHLLAKNDVLILIGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            47283..47801
                     /locus_tag="VW854_000372"
     CDS             47283..47801
                     /locus_tag="VW854_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0261668.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTGE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQVSGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            47809..48825
                     /locus_tag="VW854_000373"
     CDS             47809..48825
                     /locus_tag="VW854_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV0261669.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            48910..50166
                     /locus_tag="VW854_000374"
     CDS             48910..50166
                     /locus_tag="VW854_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEV0261670.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRPLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQVLDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            50221..51387
                     /gene="recA"
                     /locus_tag="VW854_000375"
     CDS             50221..51387
                     /gene="recA"
                     /locus_tag="VW854_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEV0261671.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEASEQDTENKKDEPKKEEAVNEEVTLDLGDELEIEIEE"
     gene            51693..53063
                     /locus_tag="VW854_000376"
     CDS             51693..53063
                     /locus_tag="VW854_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV0261672.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFAFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQSLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            53439..54395
                     /gene="lytA"
                     /locus_tag="VW854_000377"
     CDS             53439..54395
                     /gene="lytA"
                     /locus_tag="VW854_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEV0261673.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            54754..54927
                     /locus_tag="VW854_000378"
     CDS             54754..54927
                     /locus_tag="VW854_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0261674.1"
                     /translation="MYVINVCDVGKRIKELRISSNLTQDKIAEYLSLYLSLNQSMIAK
                     MEKGERNITNGFK"
     gene            54914..55120
                     /locus_tag="VW854_000379"
     CDS             54914..55120
                     /locus_tag="VW854_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261675.1"
                     /translation="MDLSNKASNLRKKLGADGEAPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMSLGR"
     gene            55166..55525
                     /locus_tag="VW854_000380"
     CDS             55166..55525
                     /locus_tag="VW854_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261676.1"
                     /translation="MKKSSVSLILIGEGDETERKADQLASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKKEMVLG"
     gene            55654..56079
                     /locus_tag="VW854_000381"
     CDS             55654..56079
                     /locus_tag="VW854_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261677.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVAKGSAEIVSIDIGTEEYALYRDLTRNHDNNKIIGKGEAASISLA
                     KKHNGILGSNNLRDVKSYVEEFSLEHMTTGDILIEAFKA"
     gene            56487..57482
                     /locus_tag="VW854_000382"
     CDS             56487..57482
                     /locus_tag="VW854_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261678.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLLFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEVPKELTPSEQLTWIAFLLTKGQKVVDVDLLSQVTGLPNSTI
                     YRCLRTFKALYWLNKQNKLYTYTVSKKELFLKSVSCLFNPIKKRILLPDGDIKQIKSV
                     SNLLYGGAYALSHSTFLAETDENISYVIWQRKFNQLSLPFSQHILKGKMLEIWKYRPF
                     VSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRIEEESEALENMILQYLGEDDAS"
     gene            57472..>57934
                     /locus_tag="VW854_000383"
     CDS             57472..>57934
                     /locus_tag="VW854_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261679.1"
                     /translation="MPANTKVIFQETFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIDEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNPEFPSMIELFSIL
                     PEYPLKKDGREIPLHFDQDASLSALLLNEDYYNILVHEKETIQGYLVLSNCG"
BASE COUNT        17343 a        10744 c        13040 g        16807 t
ORIGIN      
        1 aaaacatgtt tttgaggttg tagatgaaac tgacgaagtc agctcaaaac atgtttttga
       61 ggttgtagat gaaactgacg aagtcagtaa ccatacatac ggtaaggcga cgtgacgtgg
      121 tttgcagaga ttttcgaaga gtattagcct tccatatagt ttttcaaagc ctgtcctgtt
      181 agtccggcat tgagggcgat gaggagtttc aagcgagctt tttgggcgtt gagttcttta
      241 acaaagaaaa cgcctgcttt ttgcaactgt acgcccccac cctggtaggc ataaacaggc
      301 tcggcaatac cgttaaagca tcgtgatacc agagcgactg gaattccttt ttgcagaagg
      361 ctttctaatt tttgagccgt ttctttggga atattaccag ctccgaaggc ttggataatc
      421 aaaccgtcca agtgttctaa atccagcata tcaatcagct catctgtcat accagcataa
      481 gccgagatga tagggactaa accttgtatg tgatcaaggt caaagcgaac acgaggttca
      541 gctgttttga agtagaggat ttcctgtttc atgatgagac cgaggggacc atgtgttgga
      601 gtctggaagg tgctgacatt agtcgtatgt gttttggtga catacttggc agcgtggatt
      661 tcatcgttca taacgaccaa aactcctttg tcagcagccc tgtcatcgct ggccactcgt
      721 aaagcactta ggtaattata aacaccatca ctaccgagct cattggagct acgcatggct
      781 cctgttagaa cgataggcat atggggaact tccatggtat caaggaaata ggctgtttcc
      841 tctaaagtat cggttccgtg tgtgatcacc actccatcgt agttatctgc ttcctcttta
      901 attttctggt agaggaccag catatgtttg ggtttgatat ggggacttgg aagattaaaa
      961 aagtccaagg cgtggacttg gattccttca agtgggttgg acacatggtt catgggatta
     1021 tctgaactcg tcacaacagc gccagaagca tcggcctgca tggaaatagt tccacccgta
     1081 tgtaaaacaa ggattttctt aggcatgatt tactaactct ttctgaaatg tggtataatc
     1141 attgtatcac aaaaggggag attggaatag gatggaagtc aaagctgttt tttttgatat
     1201 cgatggaacc ttggtcaaca atcgcaagag tgttttgaaa tccactaagg acgcgattaa
     1261 gattgtcaaa gaacaagggg tactagttgg cgtagcgaca gggcgaggac ctttttttgt
     1321 taaggaattg atggacgatt tggatctgga ttttgcggta acctataatg gccagtatat
     1381 ctttagtaaa gacagagtct tgttcacgag ccctatttcc aagttacatt tgcgccatct
     1441 aattagctat gctaaaaaag agggcacgga gattgctcta gggaccaagg atgccatgtt
     1501 gggttctaaa atcatgtcct ttggtctggg ttctttttcc caacgaatta gtcgcttcgt
     1561 tccctctgtt ttaactcgga cggtgagtca gtcttttaat cgcatggtca gcaaggttgt
     1621 tccccaaaag gaagaggacc tgcttcacct gatgaatcaa cctatctacc aagttttgat
     1681 gctgatgaca ccagaagaat ctgagaaggc ggcagctgat tttgaagact tgaaattgac
     1741 acgtagcaat ccttttgcat cggatgtcat caatcaggga aattctaaat tagaaggcat
     1801 tcgccgagtt gggaaagaat atggctttga cctcaaccaa gtcatggctt ttggtgactc
     1861 agataatgat cttgaaatgc tggctggcgt cggtatgtcg gtcgctatgg gaaatggtag
     1921 tagcagtgtc aaggaagctg ccaagcacat taccactagc aatcaacaag atggaatcca
     1981 caaggccttg gaacattttg gtgttttgtc ttcagaaaaa gtctttgtca gccgtgacta
     2041 tcatttcaat aaggtcaaga ccttccacca catgatggat gaacgaaccc aagaagaacc
     2101 tcgagcttgg gatttagagg gtgcaaccca tagggttggt tttaaaatag aagaattggt
     2161 ggagtttgtt cgagcagcca gtccttctga agaagatttt ggtcaagctg tatcgcaact
     2221 tcatcaggcc cttgataagg cagcagataa agtagccaag aagacacctg ctcagcaaga
     2281 tttgataggg caagtggatg ccttgattga catgctttac ttcacctacg gcagttttgt
     2341 cttgatgggg gtggacccag aacgtatttt tgatattgtc catcaggcca atatggggaa
     2401 aatttttcca gatggtaagg ctcattttga tccagtgacc cataaaatct taaaaccaga
     2461 taattgggaa gaaaaatatg ctccagaacc tgctattaaa aaggaactgc agcgccagct
     2521 caaggcttac gaacgccata aagagagaaa taaatcgtaa acaaaaagga gctaggggct
     2581 ccttttcttt gtgattataa ggttttttct tgttctctta caaccagcaa atcgacctta
     2641 gcatggcgga gtatgtattc agatgaagag ccgaccaaga ggcgttcaaa ggcgttgaga
     2701 ccagttgcgc caacgaggat gaggtccact tcctcggcat ctggaatagt acgtgctagc
     2761 agggtctttg gatttcccat ttcaatgacg atatgaacat ctgctacccc agcatcttta
     2821 gcacgttttt cgtactcttt catcagactt tcagcgtcga cttggagttc ttcgtaaact
     2881 tcagcatcaa aggtggatac gctttggaga gcgcgtgtgt caatgacatg tgcgatggtg
     2941 agtttagcgt cgtttcgtag agcagaatga actcccttga caaaagccaa gtccgcttcc
     3001 ttagaaccat cgattgcgac catgatattt tggtaacgtt gtgccataat gaaacctcct
     3061 gaaattttct tactataatt gtaaaccttt tcactataga agtaaagcaa aaaaagtatt
     3121 tttcaaaaga atattagggc ttaaactcta tataaatata tgataaaata aaagaaagga
     3181 tagcaggaaa gaagtgaagg aagggagaat gatgaaaaac tcttttcaaa aatcaaattt
     3241 cctttattat ggaattatcc tagtttcagt cttggtagaa gtgattatga tctgtcaatt
     3301 agagagtctg gtaccgcttc tttatccaag ttttattgga tttttagttt ttcatgtact
     3361 ctaccatcta atcttatttc tagttgcaaa acgaagtggg cgttgggatt acttgatgat
     3421 atgggggctt ttcctcatgt ttaatcttct ttatgactcc tttctaggct tgcttttttc
     3481 taggtttatc ttttggtatg tgatgtgtct ttgcaaaata cctgctatct ctacgttctt
     3541 aacctcgcct ttagcaaggt ttttcttcta tacgaaacta gtaacacatt tcagtaaact
     3601 tgtcgcattc tctttctagt ggtataatgt tactatcctg atgaattgag gaaacaagat
     3661 gaaagaatat aacaagtcta gtaagttaga gcatgttgct tatgatatcc gtggtcctgt
     3721 attggaagaa gccatgcgga tgcgagcgaa tggagaaaag attttacgtc tgaatacagg
     3781 aaatccagca gaatttggct ttacagcgcc agacgaggtc attcatgact tgattatgaa
     3841 tgcgcgtgat agtgaaggct attctgactc caaagggatt ttctcagccc gtaaggccat
     3901 catgcagtat tgccagctga agaaattccc aaacgtagat attgatgata tttaccttgg
     3961 aaatggtgtc agtgagttga ttgtcatgtc catgcagggg cttttggaca atggggatga
     4021 ggtcttagtg ccaatgccag actatcctct atggacggcg gctgtcagcc tagctggagg
     4081 aaatgccgtt cactatatct gtgatgaagc tgtggaatgg tacccagata ttgacgatat
     4141 caagtcaaaa atcacttcca ataccaaggc aattgtcctt atcaatccaa ataacccaac
     4201 tggagccctt tatcctaagg aactcttgct ggagattatt gagattgccc gtcaaaacga
     4261 tttgattatc tttgcggatg aaatctatga ccgcatggta atggacggac atgtgcatac
     4321 gcctgtggcg agcttggcac cagatgtctt ctgtgtcagc atgaatggtc tgtcaaaatc
     4381 ccaccgcata gcaggtttcc gtgtgggttg gatggtcttg tctggcccta agactcatgt
     4441 taagggctat atcgaagggc tcaatatgct gtccaatatg cgcctttgct ctaacgtttt
     4501 ggcccaacaa gtcgtacaaa cttccttggg gggtcaccaa tcagtcgatg aattgcttct
     4561 tcctggtgga cgaatctatg agcaaagaaa tttcatctat aatgccattc aagatattcc
     4621 aggtttgtct gccgttaaac ccaaggcggg gctctatatc ttcccaaaaa tcgaccgcaa
     4681 tatgtaccgt atcgatgatg atgagcagtt tgtccttgat ttcttgaagc aggaaaaggt
     4741 tctcttggtt catggtcgag gctttaactg gcaggaacca gaccacttcc gtatcgttta
     4801 ccttcctcgt gttgatgagt tagcccaaat ccaagaaaag atgactcgtt tcttgaaaca
     4861 gtatcgtaga tagggcttgc attcgaaaaa gctggaaaca tttgcctaga gctattgaca
     4921 aaagtgaaag aatcagatag aatagtaaag tatgtttagt aactgatcac tttatagccg
     4981 gctaaacgaa tacaaaatct atgaggaggt attcatcgtg aaacgtactt atcaaccaag
     5041 taaacttcgt cgtgcgcgca aacacggatt ccgtaaccgt atgtcaacta aaaatggtcg
     5101 tcgcgtattg gcagctcgtc gtcgtaaagg acgcaaagtt ttggctgcat aatccaaacg
     5161 aattctatca aaaatcagta ggaactcgag tctactgatt tttatttttg taaaaaagtt
     5221 cagtagatgc aaatggattc ggaagcgatg ttacagtaga ttgaaactag aatagtacac
     5281 ctctatttct aaaacattgt tagaaatcga tttgactgtc ctgatcgatt tgtcctgtta
     5341 ttatttcatt ttactataaa gttgaagtag ctggagatgg cacagcgaca atcgtcttta
     5401 aagatggttc agctattaca attccaggaa atcaattggt agcacaagat ccaaaagcac
     5461 aagatagcac taaaccgact gctgaaaaat caactgttaa agcacctgct caaagagtag
     5521 atgtaaaaga tataactcat ttaacagatg aagaaaaagt taaggttgct attttacaag
     5581 caaatggttc agcattagac ggagcgacaa tcaatgtagc tggagatggt acagcaacaa
     5641 tcacattccc agatggttca gtagtgacga ttctaggaaa agatacagtt caacaatctg
     5701 cgaaaggtga atctgtaact caagaagcta caccagagta taagctagaa aatacaccag
     5761 gtggagataa gggaggcaat actggaagct cagatgctaa tgcgaatgca ggcggtggta
     5821 gccaggcggg tggatcagct cacacaggtt cacaaaactc agctcaatca caagcttcta
     5881 agcaattagc tactgaaaaa gaatcagcta aaaatgccat tgaaaaagca gccaaggaca
     5941 agcaggatga aatcaaaggc gcaccgcttt ctgataaaga aaaagcagaa cttttagcaa
     6001 gagtggaagc agaaaaacaa gcagctctca aagagattga aaatgcgaaa actatggaag
     6061 atgtgaagga agcagaaacg attggagtgc aagccattgt catggttaca gttcctaaga
     6121 gaccagtggc tcctaagaca acaagtgcac cgcaagcaac tgcaggaaca atgcaagatg
     6181 ttacctacca gtcacctgct ggcaaacaat tacctaacac aggttcagca tcaagtgcag
     6241 cacttgctag tcttggtcta gtggtggcaa caagtggttt tgctttgcta ggaagaaaga
     6301 ctagacgtag aaaatagaac agctagaaaa ttctattctc tacttaaagt tagattataa
     6361 gggggatttt gagaagtcat caatcctagt gatgggtgag aaaagtgaga acccaagata
     6421 atcacatact ttagctgaat aggaatattc tatcaatgta gccaatctct tctgtctcta
     6481 actgtggaat aggagatggg caatatcgga tagaaaagat agcagaatag ctctctattg
     6541 aagagaggag gggaaaccga aaaattaggt gcccctcctc ttttttggta taatagaaga
     6601 tagaaaacga ggttagaaga gatgattttt gatacacata cacacttgaa tgtagaagaa
     6661 tttgcaggtc gtgaggcaga agaaattgcc ttggctgctg agatgggtgt gacacagatg
     6721 aatattgttg gttttgataa accgacgatt gagcgtgcct tggagttggt agatgagtat
     6781 gatcagctct atgcgactat tggttggcat cctacagaag ctggtactta tacagaggaa
     6841 gttgaggctt acttgttgga taagttaaaa catcccaagg ttgtggcttt aggtgaaatt
     6901 ggcttagatt accattggat gacagcgccc aaagaggtgc aggagcaggt ttttcgccgt
     6961 cagattcagc tatctaagga cttggatttg ccttttgttg tccatacccg tgatgcgctg
     7021 gaagatacct atgagattat caagagtgag ggcgttggtc ctcgtggtgg tatcatgcat
     7081 tcattttcag ggacgcttga gtgggcagag aagtttgtgg atcttggtat gaccatttcc
     7141 ttctcaggag tggtgacttt taagaaggca actgacctcc aagaagcagc taaagagtta
     7201 cctttggaca agattttggt ggaaacagat gcgccttact tagcacctgt acccaagcgt
     7261 ggtcgtgaaa ataaaacagc ctatactcgc tatgtggtcg actttatcgc tgacttgcgt
     7321 ggtatgacga cagaagagct ggcggtagca acgactgcaa atgcagaacg aatttttgga
     7381 ttggacagca agtaatgaaa gagaaaattt ctcaagttat cgtggttgaa gggcgtgatg
     7441 atacggtcaa tctcaaacgt tatttcgatg tggagaccta tgagactcga ggttctgcca
     7501 tcaatgctca ggatatagag cggattcagc gcctgcacca acgtcatgga gtcattgtct
     7561 ttacagaccc agattttaat ggggagcgga ttcggcgcat gattatgacg gccattccaa
     7621 cagttcggca tgcctttctc aagcgagatg aagctgttcc aaagtccaag accaaggggc
     7681 gttctctggg aattgagcat gccagctatg aagacctgaa aacggctcta gctcaggtga
     7741 cagaacaatt tgaacatgag agtcagtttg acattagtcg tagcgatttg attcgccttg
     7801 gttttctagc aggggcagac agccgtaagc gtagagaata tctcggagag actctccgaa
     7861 tcggctattc caacggcaag caactcctca aacgcctaga gttgtttggg gttactttgg
     7921 cagaagtgga agaagctatg aaatcttatg agtaggaaag atgtagccgt tacaattttt
     7981 taagtttcac agtatttttc gaagcaggta gaagaggagg cgtctgatgt taattggtca
     8041 aaaaattaaa gagattcgga tagaaaaagg aattagtcgt ccagattttt gtggagatga
     8101 gcaagaactg acagttcgtc aactgtcgcg aattgaaagt ggagcttcgc aaccgagttt
     8161 gcccaagtta gactatattg ctcgccggct aggagttcca gtttatagcc ttatgccgga
     8221 tttttcagct cttccttctg cttatttaga attgaaatac cagattttac gtgaaccaat
     8281 ctatggtaaa gaagaggagt acgataagaa ggaagcgtgt ttggaagaga tttataaaac
     8341 atactttgat aatcttccta aagaagaaca attagcatgt gaagtattgc aggcgtgttt
     8401 ggatacttct agaactagaa ggcctgaata tgcagagtta atacttgagg aacatatgcc
     8461 tcagattata gaaaaagaag cttattcaat aaatgatatg ttgttgattc gtttgttttt
     8521 ttatcaaatg ctcattagaa aagatcttgc caaatttata aatcaaatcg aaaagctaat
     8581 gctctttctt ttggaacaga agaaggtaac tcaaatagag aattacttta taattagaga
     8641 tactcttatt tcaggaatgt gttgtcttga aaaggtagga gtaactgatt gttttaatga
     8701 ttatctatcg tgtttacaag aaattatgga taaaactcaa gattatcaaa agaaacctct
     8761 tgtatttatg tttttgtgga agcaagcatt aagagtagaa agagatttta gtttagctga
     8821 atcattttat cagtcttcta aaacatttgc gaagctaatt ggagatggat ttctagtaaa
     8881 gaaattgaca gaggaatggc aagaggatgt caaaaaatat ttataaacat agtgaatcag
     8941 tgacaaaaat gtccttgtcc tcgtatcaaa acagttctaa agttcgtctt tagaactgtt
     9001 tttttagata taagctaaaa ataatatgaa atagttagat tttaaggaca ttgatgtcct
     9061 tgatagtgtg ataaagatgg agtataatga ctataaaagt tcatttcagg gtgctatttt
     9121 gacaatttag agaaaaacaa ataattttag aatatcattt ggtaatataa tagtatcaac
     9181 gttgataatg gaaagaggag caagtaactc aacaatcttg taatcaaaaa tagaggagta
     9241 tcaatatgaa acaaacagtt aaaaagttag ctcttgtagc gagcattgca gcaacattag
     9301 gtggaggtgt atcagttgcc tctgcagccg ttcaataccc agaaggtggt gtttggactt
     9361 atggttcagg taacggtggt gcttactcaa actactatca cccttcaaaa taccatagtt
     9421 caacagttgt tagcagaaaa actggttcat ctgacaaggg atatgctggt gctggaggga
     9481 cttctcgtgc atggattcgt acttcttggg gagagaaagt tgcattctac tataatgttt
     9541 agaatggtat agccctattg tttagcttta cttggtcacc ttctcactaa gtgaccaagt
     9601 aactttttgg aggaaatgat gaaacgttta tttattttga tttcaatggt attggtatcg
     9661 ctttatatgg tgataacttc cgttgaccat cgagaagaga ttttatttgg taactatcct
     9721 tctgttgatg tgacaggaat gatgataaat caaccagtag ctagtcgcga agaggtgaca
     9781 gaggctttga gtcacttggc ggtagagcac aatagtctca ttgctcgtcg aatcgttgag
     9841 ccaaatgaag ctggagaaac acgctttacc tatgccactt atggtgaggg aaagcttcca
     9901 gaaggtctga ccatttcctc caaggagagt gcagaaacga gtgatttatt agggtcttac
     9961 ttgattgtat caggaagttt ggatggagtg agcttacaga ccaccttgaa agagcttggt
    10021 tatcaaggct ttgtttcgaa tggagaagat ccattttcga tagtcttact attgacggcc
    10081 acccctatgg tgctactgag tttagctatt tttctgctga cctttatgag tctgaccctg
    10141 atttatcgga tcaaatccct tcgtcaggca gggattcgct taatagctgg tgagagcttg
    10201 tttggagttg ctctcagacc agtgttagaa gatgtgagac agcttatctg ctcagtgctg
    10261 gtatccagtc ttttgggatt ggggattctc tggtatcaag gtgccttgtt tatggcaacg
    10321 gtgcaactgg tcatcattgc tcttctactt tatggattga ccttggcagg gatttctacc
    10381 ttactaagtg tcgtctatct acttggttta caggaaaata gtctggtgga tctattgaaa
    10441 gggaaactcc ctctcaaacg tatgatgaca ttgatgatgg tggggcaact cttagctgta
    10501 ttggtggtcg gatcgagtgc gacagctctc ctaccccact accgtgaaat gcaggaaatg
    10561 gagagagcta gcaataaatg gagccagtcc tcagaccgtt accgtctatc ctttggttgg
    10621 tctagtgcat ttgccgatga agaaggaacg cgtaaggata atcgtgagtg gcagacattt
    10681 actgaagaac ggttagccaa tacagactct ttttatatta tgagcaatgt tgacaatttc
    10741 tcagatggag cagaagtgga cctagatggc aatcgtctca gtgactacac accgtcaggg
    10801 aatgttatct atgtctcacc gcgctatctg atagaagaaa agattaccgt ttcttcagag
    10861 tttatggaca agatgcaaaa cttgtctgag ggagagtttg ggctgatctt gcctgagagc
    10921 ttgcgagagc agtctatcta ctaccaagga ttgtttacag attacctgca aaacttttca
    10981 tctgaaagtg tagaagtgac gagtcagaaa cactacctcc cacaggtaag gctagctttt
    11041 acagaaatag gacaggaacg tttcctctat aatgatgggt acaagacaac acgccagtac
    11101 ctaaaagatc cgattattgt agttctaacg ccgcaagcga ctggaacaag acctgttgca
    11161 gggatgttgt ggggaactac ggctaatagt gccttgaaac tagatcgata tggagacagc
    11221 atcacagctc taaaagagca aggtctgtat cacaaggttt cttacttggt aaaaagccag
    11281 ctattttttg ccaaggtact aaatgacaaa cgggtggagt tttactctct ccttattggg
    11341 acgattttga ccctgtctac ggctatcttg ttatttgatt ccatgaatct tctctatttt
    11401 gagcagttca gacgggatct tatgattaaa cgtcttgctg gtatgacaat ctatgagctt
    11461 catggcaagt atttactggc gcaaggagga gttctcttgc ttggcctagt cctatctagt
    11521 attttgacaa gagatggttt gattagcgct ctagttgtag ctttgtttac gcttaacgcc
    11581 ctcttgattt tagtaaggca ggacaaaaaa gaagaagctg gtagcatggc agtattgaaa
    11641 ggaaaataag atgattgata ttcaaggatt ggaaaagaaa tttaatgacc gcgcgatttt
    11701 ctctggtttg aatctcaagc tggagaaggg caaggtttat gccttaatcg gaaagagtgg
    11761 aagcggaaag acgacgctgc tgaatatctt gggaaagcta gaaaagatag atggaggaag
    11821 ggttctctat caggggaaag atttaaaaac cattcccact cgtgagtatt ttcgagatca
    11881 gatgggctat ctctttcaaa attttggcct cttagaaaac caatcaatca aagaaaattt
    11941 ggatttgggt tttgttggtc agaaaatctc aaaagtagaa cgtttggaaa ggcaagtggg
    12001 ggctttagaa aaagttaatc tagggtattt ggatttagaa caaaaaatct atactttatc
    12061 tgggggagag gcccaacgag ttgcccttgc taagactatt ttgaaaaatc cacccttgat
    12121 tttggcagat gaaccaacag cagctcttga tcctgaaaat tcagaggagg ttatgaatct
    12181 cttggtggat ttgaaagatg aaaatcgaat tatcatcatt gcgacccata atcccctagt
    12241 ctggaataag gctgatgaaa tcattgatat gaggaaactt gctcatgtgt gaaaaaatcc
    12301 gtattcgcag ggtatctgat tatcctagtg ccagaggtgg tttagaagat atcctcatca
    12361 tggaaaatat gaccaatcat ctccttttgg ttcaaatcct agtgcatggc tatttgcttg
    12421 attttgctag tattgaaggg taaaggcaaa agcattatcg tttgaaaaat ttacctcaaa
    12481 cggttgaact gacagtggat gatgtggagg aggatgtgga tttgacccta cccgaaaata
    12541 gaagttatca agaagctgat ttttttgata ctcaatgaaa atcaaagagc aaactaggaa
    12601 gctagccgca ggctgtactt gagtacggca aggtgaagct gacgtggttt gaagagattt
    12661 tcgaagagta tgaacgtatg tttcgagaga agcgctaagt ccctctttaa agatttccaa
    12721 gactatcttt cttcatgagg aaagatagtt ttttggtatg attttcattc ccaaaataca
    12781 aggggaatgt gttacaatag tagtaacaga taatagaaaa gagaatagat gagaattgca
    12841 gattatagcg tgaccaaggc agtgctggag cgtcacggtt ttacctttaa aaagtccttc
    12901 gggcaaaatt tcctgacgga taccaatatc cttcaaaaaa ttgtggatac ggctgaaatt
    12961 gatgaccagg tcaatgtcat cgaaatcggg ccaggtattg gtgccttgac agaatttttg
    13021 gctgagcgtg cagcccaagt catggctttt gagattgacc accgtttggt gccaattttg
    13081 gcagatactc tgcgtgattt tgataatgtg accgtagtta acgaagatat tctcaaggtt
    13141 gatttggcgc aacatatcca gaattttaaa aatcctgacc tgccaatcaa ggtagtggct
    13201 aatttgcctt actacatcac gacgcctatt ctcatgcact tgattgagag tggcattcct
    13261 ttttgtgagt ttgtggtcat gatgcagaaa gaagtagcgg accgcatttc agcccagcct
    13321 aacaccaagg cttacggtag cttgtctatc gccgtgcagt attacatgac agccaaggtt
    13381 gcctttatcg tgcctcgtac ggtctttgtg ccagcgccaa atgtggattc agccatcttg
    13441 aaaatggtgc gtcgtccaga gccagccgta gcagtagaag atgagaactt tttctttaag
    13501 gtttccaagg ctagttttac ccatcgccgc aagaccttgt ggaataactt gacaggttac
    13561 tttggtaaga ctgaagaggt caaggacaag ctgaccaagg ctttggacca ggcaggcttg
    13621 tcaccaagtg tgcgtgggga agctctcagc ttggcagaat ttgccggtct agcagacgca
    13681 cttaaagggc aaggactcta agatgcaggg acaaatcatt aaagccttgg caggtttcta
    13741 ctatgtggag agtgatggcc aggtttatca aacacgcgcg cgtgggaatt tccgtaaaaa
    13801 aggccatacc ccttatgttg gggactgggt ggatttctct gccgaggaaa attcagaagg
    13861 ctatatcctc aaaattcacg aacggaaaaa cagtctggtt cgtccgccta ttgtcaatat
    13921 cgatcaagct gtagtaatca tgtccgtcaa ggaacctgat tttaacagca atttgctgga
    13981 tcgtttcttg gttcttttgg agcacaaggg catccatccc attgtctata tttccaaaat
    14041 ggatttgttg gaagataggg gagaactgga tttttaccag cagacctatg gtgacatcgg
    14101 ctatgacttt gtgaccagta aagaggaact cctgtctttg ttaacaggca aggttacggt
    14161 ctttatgggg cagacaggtg ttgggaagtc aactcttctc aataaactcg taccagacct
    14221 caatcttgaa acgggagaaa tttcagacag tctaggtcgc ggtcgccata ccactcgagc
    14281 tgttagtttt tacaatctca acgggggtaa aatcgcagat acaccaggct tttcatctct
    14341 ggactatgaa gtatcaaggg ctgaagacct caatcaggct ttcccagaga ttgctactgt
    14401 tagccgagat tgtaagttcc gtacttgtac ccatacccat gagccgtctt gtgccgtcaa
    14461 accagctgtt gaagagggtg ttattgcaac cttccgtttt gacaattacc tgcaattcct
    14521 tagtgaaatt gaaaatcgta gagaaaccta taaaaaagtc agcaaaaaaa ttccaaaata
    14581 aggagaaacc tatgtctcaa tacaagattg ctccgtcaat tctggcagca gattatgcca
    14641 actttgaacg tgaaatcaaa cgtctagaag caactggggc agaatatgcc catatcgata
    14701 tcatggacag tcattttgta ccgcaaatca gttttggtgc aggtgtggtc gagagccttc
    14761 gtcctcatag taagatggtt ttcgattgcc acttgatggt gtcaaaccct gagcatcatc
    14821 tggaagattt tgcgcgtgca ggtgcagaca tcatcagtat ccatgtagaa gcaacgcctc
    14881 atattcatgg cgccctccaa aaaattcgtt cactcggagt taagccttca gtcgttatca
    14941 atcctggcac accagttgaa gccatcaagc acgtccttca tctagttgac caagttttag
    15001 tcatgacggt taatccaggt tttggtgggc aagcctttct gccagaaacc atggataagg
    15061 ttcgtgagtt ggttgctctt cgtgaggaaa aaggtttgaa ctttgaaatc gaagtggatg
    15121 gtgggattga tgaccaaact attgctcaag ccaaagaagc tggtgcgact gtttttgtag
    15181 caggttccta tgtctttaag ggagaagtca atgagcgagt acaaactctc agaaaacaac
    15241 tggactaggg ttgcagtttt tgcaggcgga aaccgcggtc attatcggac agattttgat
    15301 gcttttgttg gggtggatcg aggctcgctc tgggtcttgg aagaagactt acctcttgct
    15361 ctagcagtcg gagattttga ttctgtgacg gaagaagagc gacaggtgat tcaaaaacgt
    15421 gcccagcatt ttgtccaagc ccggccagaa aaagatgata ctgatttgga attggctctc
    15481 ttgaccatct ttaagcaaaa tcctcaggct caggttacta ttttcggtgc cttgggtggc
    15541 cgtattgacc atatgttggc caatgtcttt ctgcctagca atcctaagtt ggcaccctat
    15601 atgcatcaaa tagaaattga ggatgggcaa aacttgatta cttattgtcc agaaggaatc
    15661 agtcagctag aacctcgttc agactacgac tatctagcct ttatgccagt tcgggatagc
    15721 cagctgacta ttcttggagc caagtatgag ttgacagagg aaaatttttt ctttaaaaaa
    15781 gtgtacgctt ctaacgaata tatagatagg gaagtgtcgg taacttgccc agatggttat
    15841 gtggtcgtac tgcatagcaa ggacaggagg taggatggaa agtttactta ttctattatt
    15901 aattgccaac ctagctggtc tttttctgat ttggcaaagg caggataggc aggagaaaca
    15961 cttaagtaag agcttggagg atcaggcaga tcatttgtca gaccagttgg attaccgctt
    16021 tgaccaagcc agacaagcca gccagttaga ccaaaaagat ttggaagtgg ctgtcagcga
    16081 ccgtttgcaa gaagtgcgga ttgaattgca ccaaggcctg acccaagtcc gtcaagaaat
    16141 gacagataat ctcctccaaa ctagagacaa gacagaccaa cgtctccaag ccttgcagga
    16201 atcaaatgag caacgtttgg aacaaatgcg ccagacggtc gaggaaaaac tagaaaagac
    16261 cttgcagaca cgcttacagg cttcctttga gacagtttct aaacaactgg agtctgtcaa
    16321 tcgtggtctt ggagaaatgc agacagttgc ccgtgatgtc ggagctctta acaaggttct
    16381 ctctggaacc aagacgcgag ggattctggg agaattgcaa ctggggcaaa ttattgaaga
    16441 catcatgaca cctgcccagt acgaacgaga atacgcaacg gttgaaaact ctagtgaacg
    16501 agtggagtat gccatcaagt tacccggaca aggcgaccaa gaatacgtct acctaccaat
    16561 tgactctaag tttccactgg cagattatta ccgcttagaa gaagcctatg aagtaggtga
    16621 caaggatgaa atcgaacgct gtcgtaagtc actcctagca agtgtcaagc gttttgctag
    16681 ggatattagg aacaagtaca tagcaccacc tcggacgacc aattttggag ttttgtttgt
    16741 tccgacagaa ggtctctact cagaaatcgt ccgcaatccg gtcttctttg atgatttgag
    16801 acgggaagaa cagattatcg ttgcaggacc aagtacccta tcagcccttc ttaactccct
    16861 atcagttggt ttcaagaccc ttaatatcca aaagagtgcc gaccatatca gcaagactct
    16921 tgccagtgtc aagaccgagt ttggcaagtt tggtggtatt ctggtcaagg cacaaaaaca
    16981 tctccaacat gcctctggca atattgatga attattaaac cgtcgtacca tagctatcga
    17041 gcggacgctc cgtcacattg agttgtcaga aggcgagcct gcgcttgatc tactccattt
    17101 tcaagaaaat gaggaagaat atgaagatta gtcacatgaa aaaagatgag ctatttgaag
    17161 gcttttacct aatcaaatca gctgacctga ggcaaactcg agctgggaaa aactacctag
    17221 cctttacctt ccaagatgat agtggcgaga ttgatgggaa gctctgggat gcccaacctc
    17281 ataacattga ggcctttacc gcaggtaagg ttgtccacat gaaaggacgc cgagaagttt
    17341 ataacaatac ccctcaagtc aatcaaatta ctctccgcct gcctcaagct ggtgaaccca
    17401 atgacccagc cgatttcaag gtcaagtcac cagttgatgt caaggaaatt cgtgactaca
    17461 tgtcgcaaat gattttcaaa attgaaaatc ctgtctggca acggattgtc cgaaatctct
    17521 acaccaagta tgataaggaa ttctactcct atccagctgc caagaccaac caccatgcct
    17581 ttgaaacggg cttggcttat catacggcga ccatggtacg tttggcagac gctattagcg
    17641 agatctattc tcagctcaat aagagtctgc tctatgcggg gattatgttg cacgacttgg
    17701 ctaaggtcat cgagttgacg ggaccagacc agaccgagta cacggtcaga ggcaatcttc
    17761 ttggacatat cgctctgatt gatagcgaaa ttaccaagac agttatggaa ctcggcatcg
    17821 atgataccaa ggaagaagtc gttttgcttc gtcatgtcat cctcagtcac cacggcttgc
    17881 ttgaatatgg aagtccagtc cgtccacgta ttatggaggc agagattatc catatgattg
    17941 acaatctgga tgcaagcatg atgatgatgt caacagctct ggctttggtg gataaaggag
    18001 agatgtccaa taaaatcttc gctatggata atcgttcctt ctataaacca gatttagatt
    18061 gataatttaa gaaaaatgag cattttttag gataagaatg ttcgtttttt tatgtgaata
    18121 tggtataata agtaaaagac aaaaatgaat actcttcgaa aatctcttca aactagggta
    18181 gtatcgcttt gtcgtatgta tatatgcagg tatattacag ggtttgtcag ttctatcgac
    18241 aatctcaaaa cagtgttttg aaccaccagc gaccagcttt ctagtttgct ttttgatttt
    18301 ttgaataaaa atggaatagg aaatagaaat gaaattaaga agaagtgatc ggatggttgt
    18361 catttccaac tatttgatta ataatcctta taaactaact agtctcaata cttttgctga
    18421 aaagtatgag tctgctaaat catccatctc agaagatatc gtcattatca aacgcgcctt
    18481 tgaggaaatt gaaatcggtc atatccagac agtgactggg gctggcggag gtgtcatctt
    18541 cacaccgtct atttcgagtc aggatgctaa ggaaatggtt gaagacttgc gtaccaagtt
    18601 gtcagaaagt gaccgtatct tgccaggtgg ttatatctat ctgtctgatt tgcttagcac
    18661 accagccatc ttgaaaaata ttggtcgtat tattgccaaa agctttatgg accaaaaaat
    18721 tgacgcggtt atgaccgtag caactaaggg tgtgccactt gcaaatgcag ttgccaatgt
    18781 cctcaatgtc tcttttgtca ttgtgcgccg tgacctgaaa attaccgaag gttcaactgt
    18841 tagcgtcaac tatgtttcag gttcaagtgg tgaccgtatc gagaaaatgt tcctttcaaa
    18901 acgtagtctt aaggcaggca gccgtgtctt gattgtggat gacttcttga aaggtggcgg
    18961 aacggtcaac ggtatgatta gtctcttgcg cgagttcgac tcagaactgg caggtgtagc
    19021 ggtctttgcg gacaatgccc aagaagaacg tgaaaagcag tttgactaca agtcactctt
    19081 gaaggtaact aatattgatg tcaagaacca agccatcgat gttgaggttg gcaatatctt
    19141 tgacgaagat aaataagaga tagaactaaa ggttggaacg attgtcccag cctttctttg
    19201 caaacagaat agaaggaagc ttatgaaaac accatttatc aatagagaag agttagaagc
    19261 gattgttgcc gagttcccga ctccctttca cttgtatgat gagaagggga ttcgtgagaa
    19321 ggcaagagcc gtcaaccaag ctttttcgtg gaacaagggc tttaaggaat attttgcagt
    19381 taaggctact ccaactccag ctattttgaa aattctccaa gaagaaggtt gtggtgtgga
    19441 ctgctctagt tatgtagagc ttttgatgag ccataaactg gactttctgg gttctgagat
    19501 tatgttctct tccaacaaca cgccagacaa ggaatacgcc tatgcacgtg aattgggtgc
    19561 gaccattaac ttggatgcct ttgaagatat tgaacatctg gagagagcag caggtattcc
    19621 agaaatcatc tcttgtcgtt ataatcctgg aggcgttttt gaactgggga cagacattat
    19681 ggacaatcct ggggaggcta agtttggcat gaccaaggac cagctctttg aagcctttgc
    19741 tatcttgaag gaaaaaggag ccaagacttt tgggattcac tccttcctag cgtccaatac
    19801 cgtgacccat ctctattatc cagagttggc tcgtcagctc tttgaactgg ctgttgaaat
    19861 caaggaaaag ttgggcattt ggctagactt tatcaatctt tctggcggta ttggtgttaa
    19921 ttatcgtcca gaccaggagc cgaacgatat cgccttgatt ggtgagggag ttcgtaaggt
    19981 gtatgaagaa gttcttacgt cagcaggtct tggtcaggtc aagattttca ccgaattggg
    20041 tcgttttatg ctggcacctc acggtgctct agtcacaaga gtgactcata agaaggaaac
    20101 ctaccgtacc tatctaggtg tggatgcctc agcagtcaac ctcatgcgtc cagctatgta
    20161 cggagcttac catcatatta gcaacgtgac ccatccagat ggaccagctg aagcggtaga
    20221 tgtggttggt tcactctgtg aaaacaatga taaatttgca gtcaatcgcg aactgcctca
    20281 tacagaaatc ggtgatttgc tggtcattca tgatacaggt gcccacggat tttcaatggg
    20341 ctaccagtat aatgccaaat tacgttctgc ggaaatcctc tataccgaag aaggtaaagc
    20401 ccgtcaaatc cgccgtgcag agcgccctga ggactatttt gcaaccttat atggcttcga
    20461 ttttgaagaa taatctgata atagattgaa aatgaaattg aaaaacagat tgctttctaa
    20521 aaaataggca aaaatcttgt ttttccttca agtcgtgata taataaaact ataaaacgtt
    20581 ttcaaggaag gtaacgatat gtctgaagaa acaattgatt atggacaagt gacaggaatg
    20641 gtgcattcga cagaaagctt tgggtcagta gatgggcctg gtattcgctt tattgtcttt
    20701 ttgcagggct gtcacatgcg ttgccagtat tgccacaacc cagacacttg ggctatggag
    20761 tccaataagt cacgtgaacg gacggtagat gatgtcttga cagaggcctt gcgctaccgt
    20821 ggtttctggg gaaataaggg tgggattaca gtcagtggag gagaagctct cttgcagatt
    20881 gatttcctga ttgctctctt caccaaggct aaggaacaag gaatccactg taccttggat
    20941 acctgtgctc ttcctttccg taataaacca cgttaccttg agaagtttga caaactcatg
    21001 gctgtcactg acttggttct tttggatatc aaggaaatca acgaagaaca gcacaagatt
    21061 gtcactagac aaactaataa aaatatcttg gcttgtgccc agtatctatc agatattgga
    21121 aaacctgtct ggattcgcca cgtgctagtt ccaggattga cagacagaga tgatgacttg
    21181 attgaacttg gtaagttcgt caagaccctc aaaaatgttg ataagtttga aattctacct
    21241 tatcacacca tgggtgagtt caagtggcgt gaacttggaa ttccatattc cctcgaagga
    21301 gtcaaaccac caacagcaga tcgcgtcaag aacgctaaaa aactcatgga taccgaaagt
    21361 tatcaagatt atatgaaacg tgtacatgga tagaaaagaa gcctgatgga aacatcgggc
    21421 ttttgatttg caaaaagact tagcaaatca gctaagcctt tttcttctta tctcgaacgt
    21481 tgttttccag cgttgcgatt tttgtgtttt ttcttgcttg tgatagcagt tggttgttca
    21541 ggggtaacgt cttttcgtcc acttggttta gagaaagcac ttgcttttgg tgggttcttg
    21601 gctagttctt cacggacttt tttgcgaagt tttggacgaa cgatatagtt gacgataaac
    21661 tgttggagaa tcatcatgaa accaccgaca acccagtaaa gtgtgacact agctggtgag
    21721 aagagggaga agacgacgat catgagtggg ctcatgtaaa tcattttctt gatttgttct
    21781 ctttgcattt catcttctac tccgtgaagt gaaaggagcg attgaagata gtaaaggaca
    21841 ccagcacagg caaccaaaat catacttgga gaacctagag gaatgcctag gtagcttgct
    21901 tgagcaaccc cttcagtatg ttgggcagca aagtagatag cagagaagaa aggcatttga
    21961 aggaggatag ggaaacatcc tacaccacca aacatgctga tgccgtgctc tttttgagcg
    22021 gcaaagaggg cttgttgggc ttcgagtttt tcttcttgag tagttgcttc tttgagacgt
    22081 gtttggtgtg gctcaaggac atgcttgaga gcgttcatct tttcagagtg aagcgttgcc
    22141 ttccatgatt ggtagatacc aagtggtaag ataatcaagc gtacgataat ggttacgata
    22201 atgatagcga caccaaagcc tagaccttta tcagtagcga agtacttgat ggcttcagcc
    22261 ataggcgctc cgatcgtatt ccaaataaat cctgttggct gacctgtggt tttatcgaca
    22321 ttgacacagc cagtcaagac aagcaacata gccactccca tagccgagaa tgcaaaacgt
    22381 ttaatagatt tcactgtttt tattccttct ttaaaaatta tacctttcta ttctactgtt
    22441 tttttacaaa atatacaata gttctggaga cctaatttgc gattttaaag tcagagtagg
    22501 gaggaaagtt gcttagtttg acatctaagt agcttacttt tgaaaaaggt gtcggtcctt
    22561 ttcggatttc ttggataaat tttgccatga tagcagatga gtctgcttgg gctaagattt
    22621 ccactgtgcc atcgtcgtta ttccatactc gacctgtgat gccaccaatt tcaagtgcca
    22681 agctgtaaac accccaacga aagccgactc cctgtaccct accttgggca atcattctaa
    22741 ccttttgcat accaaccccc tttgattgtg ttataatatt tctatgacta ttataacctc
    22801 aaaagccaat tctgtggtaa aaaatgccaa gaaattacgc caaaaaaaat accgcaagtc
    22861 tgcctatttg attgaaggct ggcacttgtt tgaagaagct gtccaagctg gagtgacgat
    22921 tgagaaaatc tttgccctag aaaattatcg agatcagtta gctacttttt cgcaaactgt
    22981 ctgggtgtca gaggatattt tgctagattt ggcggattct cagactccac agggaattgt
    23041 ggcggtggtt caaaaagaag aagtaggaca agctgatttg agtcagggca agttcttgtt
    23101 tttggaagat gtgcaagatc ccggtaatgt aggaactatc attcgaactg cggatgcagc
    23161 aggttttaca ggagtgattg tttcagataa gtcggcagac atctacagtc tcaaaaccct
    23221 acgttccatg caaggtagtc atttccatct gcccatttac cggatgacta gtcaagcgct
    23281 tcttgacgaa actaaaaagg tagctatccc agtgctagca acaaccctat ctaaagattc
    23341 tgttgattac agagaactgc cttctataga aaattttgta ctagttatgg gaaatgaggg
    23401 tcaaggaatt agtcccctta tggctgaaag tgcagaccag ttggtccata ttagcatgaa
    23461 ggggcaggcc gagagtttga atgttgcagt tgcaaccggt attttaatct tccatttaag
    23521 ctaattttaa ctttttttgt tataatcaag gaaagatgtt cacagaaaag gagaaaagga
    23581 tgaatcacac tattatacat gaccgtgcag gtctcaatca attttacgct aaggtttatg
    23641 cctttgttgg tctgggaatc ggactatctg ctttggtatc aggccttatg ttgacggtct
    23701 ttcagtctca gttggtttac ttgttgatgc aggggcgtct ctggttgacc attgctactt
    23761 ttgcggagtt ggctttggtt ttcgttgcca gtagcatggc ttcaagaaat agtccagctg
    23821 ctcttccagt atttttactt tactccgttt taaacggctt tacccttagt tttgtggtgg
    23881 ctttctatac tccgggtaca gttttatctg cctttgtatc gagcgccctt ctcttctttg
    23941 tcatggcggc agttggtatc tttaccaaga aagacttgag tgggattggt cgagcaatga
    24001 tggcagctct cattggttta ctgattgcta tggtagtcaa tattttcttg gctagtggct
    24061 tctttgatta tatgattagc gtagccatgg ttttggtttt ttcagggttg attgcttggg
    24121 ataaccaaaa gattcgcctc gcctatgaac aatcacaagg tcgtgtagcg acaggctggg
    24181 tggtgtcaat ggctctcagc atctatcttg attttatcaa tcttttccta agtattctac
    24241 gtatctttgg tcgcaatgac taaaaggtct atgaaatcag tgttttgagt gacactgatt
    24301 ttttttaaaa ttttctcttt tcttttcttg gaaataggtg tataatacgt ttattaaatt
    24361 tttgaggagt tgtctatgaa gaaaagtttt atccatcaac aagaagaaat ttcctttgtc
    24421 aaaaacactt ttacccagta tttgaaagat aagctagaag ttgtcgaagt tcaaggtcct
    24481 atcttgagta aggtcggtga cggaatgcag gacaacctgt ctggtgtgga aaatccagta
    24541 tcggtcaagg ttctccaaat ccctgatgct acttatgaag tggtgcactc acttgctaaa
    24601 tggaaacgcc acaccttggc tcgttttggc tttggtgaag gagagggtct ctttgtccac
    24661 atgaaagccc ttcgtccaga tgaggattcc ttggatgcaa cccactctgt ttatgttgac
    24721 cagtgggact gggagaaggt tatcccaaat ggtaagcgta acatcgttta tctaaaagaa
    24781 acagttgaga agatttataa ggctattcgc ctgactgagc tagctgttga agctcgctat
    24841 gacatcgagt ctatcttgcc aaagcaaatc acctttatcc atacagaaga attggtagaa
    24901 cgctacccag acttgacacc gaaagaacgt gaaaatgcga tttgtaaaga atttggagcc
    24961 gtctttttga ttggtatcgg tggcgagttg ccagatggta aaccgcacga tggacgtgca
    25021 ccagactatg atgactggac aagcgagtct gagaatggct acaagggtct aaatggtgat
    25081 attcttgtct ggaatgagtc tttaggtgga gcctttgagt tgtcttctat gggaattcgt
    25141 gtagatgaag aaactcttag acgtcaggtg gaaattacag gtgatgaaga ccgcttggaa
    25201 ttggaatggc acaagtcttt gttgaatggc ctattcccat tgacaatcgg tggaggaatt
    25261 ggacaatctc gtatggccat gttcctactt cgcaagagac acatcggaga agtgcaaaca
    25321 agtgtttggc ctcaagaagt ccgcgatact tacgaaaata ttttgtagag aatcgaaccg
    25381 caaggttcgg ttttctttct ctttttgtct ataatttggt ataataaaca gtatgaaaat
    25441 cgtatcagga atctatggga gacgtcccct caagacacta gaaggcaaga caacaagacc
    25501 tacttcggat aaggttaggg gagccatttt taacatgatt ggtccctact ttgaaggggg
    25561 acgagtcttg gacctttatg caggtagtgg tggtttatct atcgaagcag tatcgcgtgg
    25621 catgtccagt gctgttttgg tggagcgaga ccgtaaggct cagaccatcg tggctgaaaa
    25681 tatccagatg accaaggaag ttggaaaatt tcaactcctc aagatggatg cagaaagggc
    25741 attggaacag gtatctgggg aatttgacct cgttttctta gaccctccct ataccaagga
    25801 acaaatcgta gcagatattg aaaaaatggc tgagagagag cttttttctg aagatgttat
    25861 ggttgtgtgc gagacggata aggccgttga acttccagaa gaaattgcct gtctgggtat
    25921 ctggaaggaa aagatttatg gaattagtaa ggtgacagtc tatgtcagat aagattggct
    25981 tattcacagg ctcatttgat ccgatgacaa atgggcatct ggatatcatt gaacgggcga
    26041 gcagactctt tgataagctc tatgtcggta ttttttttaa tccccacaaa caaggatttc
    26101 ttcctatcga aaatcgtaaa cgggggctag aaaaggcttt gggacatctg gaaaatgttg
    26161 aagtcgtggc ttctcatgat gaattggtgg tcgatgttgc aaaaagattg ggtgctactt
    26221 gtctagtgcg tggtttgagg aatgcgtcgg atttgcaata tgaagccagt tttgattact
    26281 acaatcatca gctgtcttct gatatagaga ctatttattt acatagtcga cctgaacatc
    26341 tctatatcag ttcatcaggc gttagagagc ttttgaagtt tggtcaggat attgcctgct
    26401 atgttcccga gagtattttg gaggaaataa gaaatgaaaa aaaagattag atggccctta
    26461 tacgtcattg cggccttgat tgtgactttc ttggcatttg tagtgccctt gccttattat
    26521 atagaggttc caggtggttc ggaagatatt cgccaagtcc ttaaagtaaa tgacacagaa
    26581 gataaggaag ctggtgccta tcaattcgtt acggttggtg ttcaacatgc cactttagct
    26641 catatgattt atgcttggtt gacacctttt acagatattc gtagtgctca ggagactaca
    26701 ggtggttctt ccgatgttga atttatgcga atcaatcaat tctacatgca aacatcacaa
    26761 aatatggcca agtatcaagg tctaaaaaca gctggtaagg atatcgaact caagtacttt
    26821 ggggtttatg tcttgaatgt gacggataat tcaaccttta aagggattct caatatctct
    26881 gatacggtca cagcagtcaa tgatcagacc tttgatagtt ccaaagactt gattgattac
    26941 gtcagttctc aaaaattagg ggactccgtc aaggtcacct atgaagagga tgggcaaacc
    27001 aagtctgcag aaggaaagat tatcaccttg gaaaatggaa aaaatggaat tggaatcggc
    27061 ttgattgatc gtacagaggt aatcagcaat gtcccaatta gcttttcaac agctggtatt
    27121 ggcggcccaa gtgctggtct catgtttagt ctagctatct atactcaaat agctcactca
    27181 gatcttcgta atggtcgtat tgttgccggt acaggtacca ttgaccgcga tggtaatgtg
    27241 ggagacattg gaggtattga taagaaggtt gtagcttcgg ctagggcagg tgctgctatt
    27301 ttctttgctc ctgataaccc tgttagcgaa gaagaacaaa aggcgcatcc ggacgcgaaa
    27361 aacaactacc aaacagccct agaagcggct aaaacaatca agacggatat gaaaatcgtg
    27421 cccgttaaaa ccctacaaga tgcgattgat tacttgaaaa acaatcccta atttttgagt
    27481 gaagtttcca aactagcgca caaacagaga agatggtata atagtcaaat ggttcaatta
    27541 ttattcactc taagtagtca tatgctcttt atttatgtga gtttttacct tttaaaggat
    27601 cttgttagat gggagaaggt tttaaaagtg acagatgaga atacaagaaa agttcgttta
    27661 ttagtagcct tttttagcat tgtcataggc tacatcctga gttctttctt tattagcctg
    27721 tatcatttgt ggcaagaagc gcttagagga ttattatgaa atcaagagta aaggaaacga
    27781 gtatggataa aattgtggtt caaggtggcg ataatcgtct ggtaggaagc gtgacgatcg
    27841 agggagcaaa aaatgcagtc ttacccttgt tggcagcgac tattctagca agtgaaggaa
    27901 agaccgtctt gcagaatgtt ccgattttgt cggatgtctt tattatgaat caggtagttg
    27961 gtggtttgaa tgccaaggtt gactttgatg aggaagctca tcttgtcaag gtggatgcta
    28021 ctggcgacat cactgaggaa gccccttaca agtatgtcag caagatgcgc gcctccatcg
    28081 ttgtattagg gccaatcctt gcccgtgtgg gtcatgccaa ggtatccatg ccaggtggtt
    28141 gtacgattgg tagccgtcct attgatcttc atttgaaagg tctggaagct atgggggtta
    28201 agattagtca gacagctggt tacatcgaag ccaaggcaga acgcttgcat ggtgctcata
    28261 tctatatgga ctttccaagt gttggtgcaa cgcagaactt gatgatggca gcgactctgg
    28321 ctgatggggt gacagtgatt gagaatgctg cgcgtgagcc tgagattgtt gacttagcca
    28381 ttctccttaa tgaaatggga gccaaggtca aaggtgctgg tacagagact ataaccatta
    28441 ctggtgttga gaaacttcat ggtacgactc acaatgtagt ccaagaccgt atcgaagcag
    28501 gaacctttat ggtagctgct gccatgactg gtggtgatgt cttgattcga gacgctgtct
    28561 gggagcacaa ccgtcccttg attgccaagt tacttgaaat gggtgttgaa gtaattgaag
    28621 aagacgaagg aattcgtgtt cgttctcaac tagaaaatct aaaagctgtt catgtgaaaa
    28681 ccttgcccca cccaggattt ccaacagata tgcaggctca atttacagcc ttgatgacag
    28741 ttgcaaaagg cgaatcaacc atggtggaga cagttttcga aaatcgtttc caacacctag
    28801 aagagatgcg ccgcatgggc ttgcattctg agattatccg tgatacagct cgtattgttg
    28861 gtggacagcc tttgcaggga gcagaagttc tttcaactga ccttcgtgcc agtgcagcct
    28921 tgattttgac aggtttggta gcacagggag aaactgtggt cggtaaattg gttcacttgg
    28981 atagaggtta ctacggtttc catgagaagt tggcgcagct aggtgctaag attcagcgga
    29041 ttgaggcaag cgatgaagat gaataagaaa tcaagctacg taatcaatcg tttactttta
    29101 gtcatcatag tactgatttt aggtactctg gctctaggaa tcggtctaat ggtaggttat
    29161 ggaatcttgg gcaagggtca agatccatgg gctatcctgt ctccagcaaa atggcaggaa
    29221 ttgattcata aatttacagg aaattaggct ggagaaccag cctttttcta aagataagga
    29281 gaaatatgaa caaaaaaaca agacagacac taatcggact gctagtgtta ttgcttttgt
    29341 ctacagggag ctattatatc aagcagatgc agtcggcacc taatagtccc aaaaccaatc
    29401 ttagtcagaa aaaacaagcg tctgaagctc ctagtcaagc attggcagag agtgtcttaa
    29461 cagacgcagt caagagtcaa ataaagggga gtctggagtg gaatggctca ggtgctttta
    29521 ttgtcaatgg taataaaaca aatctagatg ccaaggtttc aagtaagccc tacgctgaca
    29581 ataaaacaaa gacagtgggc aaggaaactg ttccaaccgt agctaatgcc ctcttgtcta
    29641 aggccactcg tcagtacaag aatcgtaaag aaactgggaa tggttcaact tcttggactc
    29701 ctccaggttg gcatcaggtc aagaatctaa agggctctta tacccatgca gtcgatagag
    29761 gtcatttgtt aggctatgcc ttaatcggtg gtttggatgg ttttgatgcc tcaacaagca
    29821 atcctaaaaa cattgctgtt cagacagcct gggcaaatca ggcacaagcc gagtattcga
    29881 ctggtcaaaa ctactatgaa agcaaggtgc gtaaagcctt ggaccaaaac aagcgtgtcc
    29941 gttaccgtgt aaccctttac tacgcttcaa acgaggattt agttccctca gcttcacaga
    30001 ttgaagccaa gtcttcggat ggagaattgg aattcaatgt tctagttccc aatgttcaaa
    30061 agggacttca actggattac cgaactggag aagtaactgt aactcagtaa aagataagcc
    30121 taaactccta tgtcacttat ggatgtagga gttcttttta ctagtttaag cagggctaga
    30181 ataggtacta ataaaaaata gtaacttcta aaactaactt ccagtttccc acaacctagc
    30241 ttttagtatg agaaaaacgc gtgaaatcag tggaaatccg tcttagacta acttccagtt
    30301 ttgggagaga gatggaagtt acgctaagaa aaaaataata tgtattggat taaattttgt
    30361 gatataataa aggataagat actattttag gagaagaact atggaagacc caagtagtca
    30421 gaatttgttg ctacaatttg ttttattgtt tatcttgacg gtattaaatg cctttttctc
    30481 agccacagaa atggccatgg tttctcttaa tcgtgcccga gttgaacaaa aggcggaaga
    30541 aggagacaga cgctacatcc gcctgctgaa ggtactagaa aatcctaacc actttttatc
    30601 aaccattcag gtaggaatca ccctgattac aatcttatca ggggcgagtt taactgatac
    30661 tctaggacgt gtgattgcct cttggcttgg gaatggcgaa acagcacaag ccgtggcaac
    30721 ttttctatcc ttggcatttt tgacctatat ttccatcgtt tttggggaat tatatcctaa
    30781 gagaatcgct cttaatctaa aggatgcctt ggcaattcgt acagcgccga ttatcattgg
    30841 gatcggaaaa ctagtcagtc cttttgtttg gcttttagct gcttcgacca atttcttgag
    30901 ccatttgact cctatgtcat tcgatgatgc ggatgaaaag atgacccgtg atgaaattgc
    30961 ctatatgctg acaaatagtg aagaaacatt ggatgctgat gagattgaga tgctacaagg
    31021 tgtcttttcg ctcgatgaac tgatggcacg agaggttatg gtgcctcgaa cggatgcctt
    31081 tatggtggat attcaggatg atagtcaagc cattatccaa agtattttaa aacaaaatta
    31141 ttctcgtatc ccggtttatg atggggataa ggacaatgta attggaatca ttcacaccaa
    31201 gagtctcctt aaggcaggct ttgtggacgg ttttgacaat attgtttgga agagaatttt
    31261 acaagatcca ctttttgtac ctgaaactat ttttgtggat gacttgctaa aagaactgcg
    31321 aaatacccaa agacaaatgg caatcttgct ggatgaatac ggtggtatgg ctggtttggt
    31381 gactttggaa gacctcttag aggaaattgt tggtgaaatt gatgatgaaa cggacaaagc
    31441 agcaatagat gttcatcaga ttggcgagga tacctacatt gttcaaggaa ctatgaccct
    31501 caatgatttc aataattact ttgatgttga actggaaagt gatgacgttg ataccatcgc
    31561 tggttattat ttgacaggag tgggaactat tccaacgact gagaaactca gctatgaatt
    31621 ggtcagccaa aacaaacagt ttatcttaac caatgataaa gtgaagaatg gacgtgttac
    31681 caaggtaaaa gtccaaatca cagaagtcga aatagaagaa gaaacagaat aaactagagg
    31741 cttttgtcac caggcgtgac gaagcttttt tcaattgatt tcaaaaggga attatgccat
    31801 ggaaagaact ctgccacaaa ctcgctccaa aagtttttaa agttatacgt atttattcga
    31861 gggaaaataa gaaaagccct tcaaattggg ctttctgttc atttgaaacc tgataaatca
    31921 agttatagaa ggcggtagac ggatttgaac cgacgatcaa gcttttgcag agccgtgcct
    31981 taccacttgg ctataccgcc ttaacgttta ttattatacc ttgaaaatga ttttcagtca
    32041 atagtttttt cagttttttg taccatagcg tgattcgaat gtgatgccaa gttttctatg
    32101 ggaaaaaatt taccaaaaac ctactgaaac cccttgaaaa atctagctaa atagggtata
    32161 atatgggtaa tcatttgtcg taggttttgt ctgaaatatt gtccagacaa ggctcacagc
    32221 agttaaatct tctgaaaaag tcagatttag ctgctctttt tgtgcttttt ttcaagatta
    32281 tgagcatttg taacagaggc ttaaagattc tgaaaattcg tcaagaggac acggtgataa
    32341 ggggtttaca accatatgac gattagaaaa gcctgattga caaggcttgg aacttattta
    32401 caaaggagaa tcatcttggc aggacatgac gttcaatacg ggaaacatcg tacccgtcgt
    32461 agtttttcaa gaatcaaaga agttcttgac ttaccaaatt tgattgaaat tcaaactgac
    32521 tcattcaaag ctttcctaga ccacggtctt aaggaagtgt ttgaagatgt attgccaatt
    32581 tcaaacttca cagacacaat ggagttggaa tttgttggat atgaaatcaa ggaaccaaaa
    32641 tacacgctag aagaagctcg tatccacgat gctagctact cagcaccaat ttttgtaacc
    32701 ttccgtttga tcaataaaga aacaggcgaa atcaagaccc aagaagtttt ctttggtgat
    32761 ttcccaatca tgacagaaat gggtactttc atcatcaatg gtggtgaacg tattatcgtt
    32821 tctcagttgg tccgctcacc aggtgtttac tttaacgaca aagtagacaa aaatggtaag
    32881 gtgggctatg gttcaactgt tatccctaac cgtggagctt ggttggaact tgaaagcgac
    32941 tcaaaagata tcacctacac tcgtatcgac cgtactcgta agattccatt tacaaccttg
    33001 gttcgtgctc ttggtttctc aggtgatgat gaaatctttg atatctttgg tgacagcgaa
    33061 ttggttcgca acactgttga aaaagatatc cacaagaatc caatggactc tcgtacagac
    33121 gaagccttga aagaaattta cgaacgcctt cgtccaggtg agcctaagac ggctgaaagc
    33181 tcacgtagct tgcttgtagc tcgcttcttt gacccacgtc gttatgactt ggcagcagtt
    33241 ggtcgttaca aaatcaataa aaaactcaat gttaaaacac gtttgctcaa ccaaaccatt
    33301 gcagagccat tggtagaccc tgaaactgga gaaatcttgg tagaagctgg tacgattatg
    33361 actcgtagcg tgattgaaag cattgaaagc catttggatg gcgacttgaa caagattgtc
    33421 tacatcccaa acgatgcagc cgttgtgact gagcctgttg ttcttcaaaa attcaaggtt
    33481 attgctccaa ctgatccaga tcgcgtcgta acgatcattg gtaatgctaa cccagatgac
    33541 aaggttcgta cggtgactcc tgcagatatc cttgctgaga tgagctactt cctcaacttg
    33601 gctgaaggac ttggccgtgt agatgatatc gaccaccttg gaaatcgtcg tatccgtgcg
    33661 gttggtgaat tgcttgccaa ccaagtacgt ttgggacttt ctcgtatgga acgtaatgtc
    33721 cgtgaacgta tgtctgttca ggacaatgaa gtcttgacac cacaacaaat tatcaatatt
    33781 cgtcctgtaa cagctgcagt taaagaattc tttggttcat cacagttgtc acagttcatg
    33841 gaccaacaca acccgctttc tgagttgtct cacaaacgcc gtttgtcagc cttaggacct
    33901 ggtggtttga ctcgtgaccg tgccggatat gaagtgcgtg acgtgcacta cactcactat
    33961 ggtcgtatgt gtccaatcga gacacctgaa ggacctaaca tcggtttgat caataacttg
    34021 tcatcttacg gacacttgaa caaatatggt tttgttcaaa caccataccg taaggttgac
    34081 cgtgaaacag gtgttgtcac gaacgaaatt gtttggttga cagctgatga agaagatgaa
    34141 tatactgtag ctcaggctaa ctctcgtctg aatgaagatg gaacctttgc tgagaagatt
    34201 gtcatgggac gtcaccaagg ggtcaaccaa gagtatccag ctaatattgt tgactacatg
    34261 gacgtttcac caaaacaggt agttgccgtt gcgacagcat gtattccttt cttggaaaac
    34321 gatgactcca accgtgccct catgggagcc aatatgcaac gtcaggctgt gccattgatt
    34381 aatcctcagg caccttacgt tggtactggt atggaatacc aagcagccca cgattctggt
    34441 gcggctgtga ttgctcagta tgatggtaaa gttacttacg cagatgctga caaggtagaa
    34501 gttcgtcgtg aagatggttc attggatgtt taccacatcc aaaaattccg tcgttcaaac
    34561 tcaggtactg cttacaacca acgcactctc gtaaaagttg gtgatgtcgt tgaaaaaggc
    34621 gatttcatcg ctgacggacc ttctatggaa aatggagaaa tggcgcttgg acaaaaccca
    34681 atcgttgcct acatgacttg ggaaggttac aacttcgagg atgccgttat catgagcgaa
    34741 cgcttggtga aggacgatgt ctacacatct gttcaccttg aagaatacga atcagaaacg
    34801 cgcgatacaa agcttgggcc tgaagaaatc actcgcgaaa ttccaaacgt tggtgaagat
    34861 gccctcaaag accttgacga aatggggatt atccgtattg gtgctgaggt taaagaaggt
    34921 gatattcttg taggtaaagt aacacctaag ggtgagaaag atctttcagc tgaagaacgt
    34981 ctcttgcacg ctatctttgg agacaagtct cgtgaagtgc gtgatacttc tcttcgtgta
    35041 ccacacggtg ccgatggtgt cgttcgtgat gttaagatct ttacacgtgt aaatggagat
    35101 gagttgcaat caggtgttaa catgttggtt cgtgtttaca tcgctcaaaa acgtaagatt
    35161 aaggtcggag ataaaatggc cggacgtcac ggaaacaaag gggttgtctc tcgtatcgtt
    35221 cctgtagaag acatgcctta ccttccagac ggaactccag tcgacatcat gttgaaccca
    35281 cttggggtgc catcacgtat gaatatcggt caggttatgg agcttcacct tggtatggca
    35341 gctcgtactc ttggtattca cattgcgaca ccagtctttg atggagcaag ttctgaagat
    35401 ctttggtcaa ctgttaaaga agcaggtatg gatagcgatg ccaagacaat cctttacgat
    35461 ggacgtacag gtgaaccatt tgataaccgt gtttctgttg gagtcatgta catgatcaaa
    35521 ctccaccaca tggttgacga taaattgcat gcgcgttcag tcggacctta ctcaactgtt
    35581 acccaacaac cactcggagg taaagctcag tttggtggac aacgtttcgg tgagatggag
    35641 gtttgggctc ttgaagccta cggtgcgtca aatgtccttc aagaaatctt gacttacaag
    35701 tcggacgata tcaacggacg tttgaaagcc tatgaagcta ttacaaaagg caaaccaatt
    35761 ccaaaaccag gtgttccaga atccttccga gttcttgtca aagaattgca atctcttggt
    35821 cttgacatgc gtgtcctaga cgaagatgac caagaagtgg aacttcgcga cttggatgaa
    35881 ggaatggacg aagatgtcat ccacgtagat gaccttgaaa aagcccgcga aaaagcagcc
    35941 caagaggcta aagcagcctt tgaagctgaa gaagctgaga aagcaacaaa agcggaagca
    36001 acagaagaag ctgctgaaca agaataagca gttcacttag aatagaaagg gaagaaatag
    36061 tggttgatgt aaatcgtttt aaaagtatgc aaatcaccct agcttctcca agtaaagtcc
    36121 gttcatggtc ttatggagaa gtcaaaaaac ctgaaacaat caattaccgt accttgaaac
    36181 cagaacgtga aggactcttt gatgaagtga tctttggtcc tacaaaagac tgggaatgtg
    36241 cttgtggtaa gtacaaacgc attcgttaca gaggaattgt ttgtgaccgc tgtggggttg
    36301 aagtaacgcg tacgaaagtt cgtcgtgagc gtatgggaca tatcgagttg aaagctcctg
    36361 tatctcacat ctggtacttc aaggggattc caagccgtat gggcttgacc cttgatatga
    36421 gccctcgtgc cctcgaggaa gttatctact ttgcggctta tgtggtgatt gatcctaagg
    36481 atacaccact tgagcacaag tctatcatga cagagcgcga ataccgagag cgcttgcgtg
    36541 aatatggtta tggttcattt gttgccaaga tgggtgcgga agccatccaa gaccttttga
    36601 agcaagtaga tcttgaaaaa gaaattgctg aactcaaaga agaattgaaa acagctactg
    36661 gacaaaaacg tgtcaaagcc atccgtcgtt tggatgtttt ggatgccttt tacaagtctg
    36721 gaaacaaacc tgaatggatg attcttaaca tccttccggt tatcccacca gatcttcgtc
    36781 caatgttgca gttggatggt ggccgttttg cctcatctga cttgaatgac ctttaccgcc
    36841 gtgttatcaa ccgtaacaac cgtttggctc gtttgcttga gttaaatgca ccaggtatca
    36901 tcgttcaaaa tgagaagcgt atgcttcaag aagcagttga cgctttgatt gacaatggtc
    36961 gtcgtggtcg tccaatcaca ggaccaggta gccgtccatt gaaatcattg agccacatgc
    37021 ttaaaggtaa acaaggacgc ttccgtcaaa acttgctcgg taaacgtgtt gacttctcag
    37081 gacgttccgt tatcgccgtt ggtccaactc ttaagatgta ccaatgtggt gtgccacgtg
    37141 aaatggcgat tgaactcttt aaaccatttg tcatgcgtga aatcgttgcc cgtgatatcg
    37201 tgcaaaacgt caaagcagct aaacgcttgg tggaacgcgg agatgagcgt atctgggata
    37261 tccttgaaga agtgattaaa gaacacccag tgcttttgaa ccgcgcaccg acccttcacc
    37321 gtttgggtat ccaagccttc gagccagtct tgattgatgg taaggctctt cgcttgcacc
    37381 cacttgtctg tgaagcctac aatgctgact ttgacgggga ccaaatggcc atccacgtac
    37441 cactttcaga agaagcacaa gcagaagctc gtatcctcat gctagctgct gagcacatct
    37501 tgaacccgaa agatgggaaa ccggtagtta ctccatctca ggacatggtt ttgggtaact
    37561 actacttgac catggaagaa gctggtcgcg aaggtgaagg aatggtcttc aaagaccgtg
    37621 acgaagcggt tatggcttac cgcaatggtt atgttcacct ccactcacgt gttggtatcg
    37681 caacagacag cctcaacaag ccttggacag aagagcaaag acataaggtc ttgcttacaa
    37741 cagttggtaa aattctcttc aacgatatca tgccagaggg gctaccatac ttgcaagaac
    37801 caaacaatgc caacttgaca gaaggtgttc cagctaaata cttcttgcca cttggtggag
    37861 ctatcaagga agctatcagc aatcttgagc tcaaccctcc attcaagaag aaaaacctcg
    37921 gaaatatcat cgctgaaatc ttcaaacgtt tccgtacgac agaaacttct gccctacttg
    37981 accgcatgaa gaacctcggt taccaccact caactcttgc aggattgaca gtgggtattg
    38041 ccgatatccc agttgttgat gacaaggctg aaatcattga agaatcacac aaacgtgtcg
    38101 aacaaatcac caaacaattc cgtcgtggta tgatcacaga cgacgagcgt tacaatgctg
    38161 ttacagctga atggcgtgct gcccgtgaaa aacttgagaa acgcttgatt gctaatcaag
    38221 atcctaagaa cccaatcgtt atgatgatgg actctggagc ccgtggtaac atctcaaact
    38281 tctcacagct tgccggtatg cgtggtctga tggctgctcc gaatggacgt atcatggaat
    38341 tgccaatcct ttcaaacttc cgcgaaggtt tgtcggtatt ggaaatgttc ttctcaactc
    38401 acggtgctcg taaaggtatg accgatacgg cccttaagac agccgactca ggttacttga
    38461 ctcgtcgttt ggtcgacgtt gcccaagacg ttatcatccg tgaggacgac tgtggaactg
    38521 accgtggtct cttgatccgt tctatcgcag aaggaaaaga gatgatcgag tctctcgaag
    38581 agcgtctcaa cggtcgttac actaagaaaa ctgttaaaca tccagaaact ggtgcagtga
    38641 ttattggtcc aaatgaattg attacagaag acaaggcgcg tgaaattgtc aatgctggtg
    38701 tggaagaagt gactatccgt tctgtattta catgtaacac tcgtcacggt gtctgccgtc
    38761 actgttacgg tatcaacttg gcgactggtg atgcggttga agttggtgaa gcagttggta
    38821 caatcgctgc ccaatctatc ggggaacctg gtacacagct tacaatgcgt accttccaca
    38881 caggtggggt tgcctcaaat accgatatca ctcagggtct tcctcgtgtc caagaaatct
    38941 ttgaagcccg caatcctaaa ggggaagcgg ttattacaga ggttaaagga caagttactg
    39001 ctatcgaaga agatgcatca actcgtacca agaaagtctt tgttaagggt gaaactggcg
    39061 aaggtgaata tgtcgttcca tttacagctc gtatgcgtgt cgaagttggg ggccaagtag
    39121 cgcgtggtgc tgctctgaca gaaggttcta tccaaccaaa acgtctcctt gcagttcgtg
    39181 atgtcttgtc agttgaaacg taccttctcg gtgaagtaca aaaagtttac cgtagccaag
    39241 gggtagaaat cggtgacaaa cacatcgagg taatggttcg tcaaatgatc cgtaaagtcc
    39301 gtgtcatgga tccaggtgat acagatcttc tcatgggtac cctcatggat atcaatgact
    39361 ttacagatgc caacaaagat gtccttatcg caggtggagt tccagcgaca ggtcgcccag
    39421 tccttatggg aattaccaaa gcctcacttg aaacaaacag tttcttgtca gcggcttcct
    39481 tccaggaaac aactcgtgtc cttactgacg cagctatccg tggtaagaaa gaccatctcc
    39541 ttggacttaa agaaaatgtt atcatcggta agatcatccc agctggtact ggtatggccc
    39601 gttaccgtaa ccttgaacca catgctgtca acgaagaaga ataccttaat cctccagtag
    39661 aggaagaagg aaatgaagaa acaacagaag tagttgtgga tactgccgtt gaaactgtgg
    39721 aagaaacagt agaataagag agaatgagag aacccgaggg ttctctcatt ctctcttctg
    39781 aaaactttct ccatttttcc acgaaatgtg gtaaaataaa agaaagaagc tgacaaagga
    39841 gacgggtatg gaacaaacat tctttatcat caaaccagat ggtgtaaaaa gagggctagt
    39901 gggtgaagtg ttaaagcgca tcgaacaacg tggatttaca atcgaaaaat tggagtttcg
    39961 ttcacaggtt tcagaagagt tgattgacca gcactatcag gacttggttg gtcagagttt
    40021 ttacccaccg attcgtgaat tcatgacttc aggtccagtt cttgtgggtg tcatttctgg
    40081 tcccaaagta atcgaaactt ggcggaccat gatgggtgca actcgtccag aagaagcttt
    40141 accaggcact attcgaggtg attttgcaaa agctgctgga gaaaatgaga ttatccaaaa
    40201 tgttgtacat ggttcagatt cagaagagtc agctaagcga gaaattgctc tttggtttta
    40261 agagtggatt ggctcaatca attggttaaa agctcatttg aatagaaagt atagtcaatt
    40321 agtttaagac atgacgcatg atatcaaact ttttagtttt tgatatggtg cgttttttga
    40381 ttaagtgact attagtttgt ctcgctccgt taggtgcgag acaaaaaaaa cacccgctat
    40441 gcgggtgtgc gtcgaaggtt ataccaaaaa aactccaaac gcgatacaat aaaggtgttc
    40501 aatccaattg taaagcgaaa ggagaaaaat atggcacaaa aggttctttt atcctgttct
    40561 tgtttcaatt gactatattt ctatactttt tcttcctttc tctgtgaaaa tcgcaagttt
    40621 ttcccacctt ttctcaatac tgaaacaaca tctttccaga acttcaataa tttctttttg
    40681 tgttaaatca ttatttaact aaatctttgg aggcaatacg ttttcaaagc gactttttta
    40741 ccataggttt tgagccttca acacagatta ctaagccaaa aatgctctaa atttttgaaa
    40801 ttctaacaaa ttccactcaa ctttatcatc atctttgact ttcacaatat cttcctcaaa
    40861 ataacatgga ataaagatag ttttcttttg aaacggttgt aggaggaaaa tctctcctac
    40921 actattattg attctgagag gaaattgcaa aatatcatct accaaatttt caattattaa
    40981 gattttttgc agtttatctg aattagaatc gcaaggcttc tcaataatat aattgtcgaa
    41041 ttttttcatt ctaacttcct ctctactatt tcttccttta tcaagaaaaa gttacctcaa
    41101 tattaataaa gtacaattct aaattaactc caattcttga ttcatgataa tatttcagta
    41161 actcatcgtg caattaaaaa caaggatttt tatttcagac gatccatcgt gaacacttca
    41221 tcagccatct cccaaattgc cggattatgt gttgcgataa tgattagcct attatcatct
    41281 cgaagagata gcaaaatctc cataatcaac tgagaagttg ctgggtctat tgaagctgtt
    41341 ggctcatctg ccagaataaa gggtggattc tttaagataa cttttgccaa ggcaacccgt
    41401 tgcgattctc cgcccgataa ctcaaagatg cgcttatcta ggttaagata agccaggccg
    41461 acctgttcta aagcctgctt ctgcctcaac cgctgttccg accgactcaa cttttgacca
    41521 atgagaccta gcttaaggtt ttcttcaata ctttggtttt caattaagcc aaagttctgg
    41581 aagaggtagc ccaattcgtg acggaaaaaa tcactcgatt tataattggc caagtcttta
    41641 cctcggtaaa aaatcgtccc atcataaggt tctaatttcc caatcatgtt catcaaggtt
    41701 gtttttccgc taccacttga accaattaag gcatagactt ttccagcctc aaatgtcatt
    41761 gaaagattcg aaaataactc tcgttctcca aaagatttac tcacctgttt aagttcaatc
    41821 atattaacct cccttcaaaa caagcatgga catcttgttt tctttctgca tttggacatg
    41881 taactgtaaa agagatagac cagtaaagag taacaagact aagaaagcaa ctcctatctc
    41941 tacctgaaga aatacactcg caacaaatcc cagtaaaaac acacccagtt gagcaaagag
    42001 ataagtgcga tggatttcta agaacctgag acctgcaatg cgtttgataa aaatggcacg
    42061 tctaaattct tcaaagtaga gcctattcat agtgttaaac agcaagattg aagttgcaat
    42121 cccaagcaca gctcctgcta gcattaccca acgttccctc tggatattat ccaataatgt
    42181 gatgtagttg tggtaacctg tttgcatttc tgagacccaa ttttcaatgc cttgtctctg
    42241 gataagctcc tgggcatcag acaattgatt aaagagaacg tagttctgta ctgcgtctat
    42301 ccaaaacaaa atggactgtg gaccagttga ttggggtgtt ataacaatga tgattggatc
    42361 tttcaaaaac tgctggtaag aaataggggt cgtattatac acaaaacgat cctgacctga
    42421 ttctaaataa cctaccgtag cagtcatttg ctgtcgttca tcttgactag acatgcgact
    42481 ggttaagtcg tcttcaaaaa cagatttata atgttcttcc tctgaacgga ggtgttcagg
    42541 cagcaataag acaaactccc ctacattaag gtgattcatc ttttgcttaa tagttgtatc
    42601 tacaggaatg ttttgacgct ccaagtaatg cggtgtgacg ataaggacat tgccattcgg
    42661 gctgtaatcg tgccattctg tagaggttgt caagtttttg gaggaagcca tgccattttg
    42721 caaagtacgg tcaattaact ggtgtctaga taagaaagcc ttttgttctg aaacagccag
    42781 atccatcaat tgataccaag ttctgagttt cctttgagct tgttcatcaa aaccaggact
    42841 cgttccttca cggctgattg atagggtaat cagttgtctt tcctgactcc aaacctcttg
    42901 tccaatccga tgctgttgcc aggcttggga atactttaag ctactcccta ttcccagcgt
    42961 tacaataata atcgctaaca gttgaccgat taaaatcaaa ctaatgattc ctctgacagg
    43021 cacttgccct tttataatct gcatcaggtg tatctttttt atcccaactg cgaagagttg
    43081 agcgaaaaac agggagatcg acaacaagat gagattataa ctgagcaagc ctgctcctat
    43141 ggtcattagg gattgcgttg gaagcgacag aattttttgc ataagaatgg cgagcacgcc
    43201 agctaaactg aaaccaacag ctatcccttt caaatcctca ccaactggtc taaggaaaat
    43261 ggaccaccgt ttctctcctg aaatgaggcg aatgcccgat gaccgtaatt ggctaatttg
    43321 actaattaaa gtcagtgcta caaaggttaa gatgaaaatc aatagactga ttaactgaaa
    43381 tccgttacta aagatagcca ttaacgtaga tagtttagat ggaattgtca ggtgcatgtt
    43441 agtcaagcca agttgactta gctcttccct tagcaagtga atatctaggt gtccatcgaa
    43501 tacaaaatag tttgtttcaa cactactact ttgagcatct tctagatttt tttcctgaag
    43561 cccatcaggc aactttccgt ccccaaaggt cgtataagaa aaaactgtag taccttctga
    43621 gttaggatct tggtgttgaa tcgcaataaa gctatctgtt tcttctgcta gtttttccaa
    43681 gcgtgtagca acatgctgaa aagttgtttc aggggaagaa tcacgtacaa caacgttggg
    43741 gtaataatgg gagacatatg tatcagtcca aatggtaaat acccaaacaa agaacaaact
    43801 agcaagcagg ttggatatga gtataaataa ttttttcata gtgcattcct tattctaaaa
    43861 cagagggcag aaatactcct gccctctata aactaacaag cttactgaat tattgataaa
    43921 agaatcttct tcaattattc tttaccatcc cgcatcaaat gtaacttgct ccccaaaatt
    43981 tgtattaaca aacgcatagg ccgcactacg tgctcctgca taaactgttt tttgattgtt
    44041 acgtccatta cttccaacat aggcccagtg atactgtgaa ccatgataat aattcgaaaa
    44101 tgctccccaa ttatttggat catgatggcc tccataggtc cactcgccac cacggtgaga
    44161 aactgccaaa acaggaagag ccaaactaaa tgccaaaacg gcaagggatg atgcaactac
    44221 ttttctaaat tttaacatat tattactcct ctgaatgaaa tattatgaaa atgagcgaaa
    44281 tgcaactata tattttaatt agaaatctca gtctgaatta tagtatccta ttttttcctc
    44341 ctttctaact gcacgttcct ttctacaggc agagacctaa ttttataaac caatcttaaa
    44401 tactttagat tttgtaactc ataattttca acagttggtt agctatgaaa gcattttatc
    44461 aaacatctcc attcttatat ctatctggta ttgttacaag ctctcatcag aatacctttc
    44521 tctaaatagt tgaattttaa agtaatataa aatcaatcag ccttcccaac atcaagacca
    44581 ttggacttct tttgcgattg cactcgaatc tccaattgtt tattcttctg aataaactgt
    44641 tggtaaggaa ttgtattact aaacataaaa acacctagca atgcaaatgt aactaatttt
    44701 ttaattccac gttttttcca tgcaatgaaa cccctttctt tttattctaa cagatatatt
    44761 ttatttagtc aagtgttttt attatatttt gaactttttt attattacta tagagaatta
    44821 tttattcctt taacttgcta ttttgaacta aaaattttat aatgaaatta agcaaacagg
    44881 aaggtttatt atctttaatg aatacactcg cagagaaatt cagattgaaa agaaaagagt
    44941 tgagactctc ccagcaaact cttgcagaag gaatttgtga acaaagccag attagtaaaa
    45001 ttgagagagg gcatttcatt ccctccgcag accttttgtt caaactctca caacgacttg
    45061 aagtgccatt agattatttt tttaatgaac aaattgaaat taaatctaac ctctctaatt
    45121 tcaagcaatt atctgctcgc ctattagatg atagaaatta tgaaaatttg gaatatattt
    45181 atagaataga gattgaacga agtacttttc taacactaga agaccgaact taccttgaat
    45241 ggattaaatc tataattgac ttctatcaat atgacagtaa gtgtgaagca atttcttcat
    45301 tggaaaatat attattaaaa gtctcttcaa atactctgat ttatttaaag gcgttgaata
    45361 ctctatctaa tttctattcc ttagtgggtc gtgaacaaga atatgaggca aactactctc
    45421 atttaataga gttgtatcag acaaaaaatt tggaccatca agagttttta tttggctaca
    45481 tcagagttcg ttacaactac gctcactacc tagtatcaaa ggaaaaatat aatgaagcca
    45541 tccaagaagc tcttgaaacg attgaactct gtaaacaaag acagacaagc taccaattgg
    45601 ctcctctact tattcttgta ggaaatgctg gagccaaatt tctagacaga gaacaagtca
    45661 aaaattatta tatagaagca agagagttat gtaagattta taacaatcct ttaatgttga
    45721 tgaagataga aaattatttg aaggaactag atactgttta gttaatcttg acattatagt
    45781 ttttctgaaa cgttaaataa cctgtaaggc tgatagttag attaatacta tcagccttat
    45841 aattatgtga ctctgagtcc gtctcgctcc gtgaggtgcg agacaaaaca accacccgtt
    45901 tgacggattt tcatgatttc aaatttatat tcttcgaaaa tctcttcaaa tcacgtcagc
    45961 tctatctgca atctcaaaac agtgttttga gcaacctgtg gctagctacc tagtttgatc
    46021 tttgattttc attgagtatt agtctctagt gtttgtttag cgtcttaaag agacatttga
    46081 gaagattaga aacagttctc tcttttctcc tagtataatg attggtagga agggagagag
    46141 aagatgaaat caatgagaat cttatttttg ttagctttaa ttcaaatcag tttgagtagc
    46201 tgtttcttat ggaaggaatg catcttgtcc tttaaacaaa gtacagcttt tttcatcgga
    46261 agcatggttt tcgtttcagg aatctgtgct ggagtaaatt atctttatac ccgtaagcaa
    46321 gaagtccata gtgtcctagc cagtaagaag tcggtgaagc ttttttacag tatgttactc
    46381 ttaattaatt tgttaggagc tgttcttgtt ttgtcagata acttgttcat caaaaatacg
    46441 ctgcagcaag aattagttga ctttttattg ccatccttct ttttcctatt tgggctagat
    46501 ttgctgattt ttttaccctt gaaaaaatac gtgcgcgatt ttcttgctat gctagacaga
    46561 aaaaagacag tgttggtgac tattttagca acacttcttt tcttaagaaa tccaatgacc
    46621 attgtctcac ttctgattta tattggactg ggcttgtttt ttgcagccta tcttgtccca
    46681 aattcggtta agaaggaagt ttccttttat ggtcatattt tccgagatct tgtattggtc
    46741 attgttacgc tcattttctt ttagagaaag cctgtttttt atggatgtta tgatgataag
    46801 tttagtagga aaatagacac aaaagaaaag tttttggtat aataagagta tgtacataaa
    46861 aaatgaagaa gagttgcaag ccttagggga gcgtttgggc catctattag caaagaatga
    46921 tgttttaatc ttaattggag aactgggtgc aggtaaaacg acctttacta aaggacttgc
    46981 aaaaggatta cagatttctc aaatgattaa aagtcctacc tatactatcg tgagagagta
    47041 tgaaggtcga cttccacttt atcacctaga tgtttatcgt attgaaggag atgctgattc
    47101 tatcgacttg gatgagttta tctttggtgg cggcgtgact gttattgagt ggggaaatct
    47161 cttaggagat gccttgccag atgcttattt agaattggaa attctaaaag aagcagatgg
    47221 acgccgttta aattttcagg caaagggttt gcgtgctgag aaattgttag aggagcttca
    47281 atatggagta tgaattgctc attagggaag cagagcccaa agatgcagct gaattagtgg
    47341 cctttttaaa tcgtgtgagt ttggagacag actttaccag cctagacgga gatggtattc
    47401 tcttgactgg tgaggagatg gaaatattcc tcaacaagca agctagttcg gacaatcaga
    47461 taaccttact tgcattctta aatggcaaaa ttgctggtat tgtaaatatt acagctgacc
    47521 agcgtaagag agtccgtcat attggagatc tcttcattgt gattggaaaa agatattgga
    47581 ataatggctt gggaagtttg ttgctagaag aagcgataga gtgggcacaa gtaagtggca
    47641 ttctgcgtcg tctccaactg actgtccaaa ctcgtaatca agcagcagtc catctttatc
    47701 aaaagcatgg ctttgtcatt gaaggtagcc aagagcgtgg ggcatatata gaagaaggga
    47761 aatttatcga tgtttacttg atgggtaaac tgataggtta gtagaaatat ggttaaaaaa
    47821 attattggaa tggtgctagc tttactttct gtaactgtag taggagtagg tgtttttgct
    47881 tatactattt atcaacaagg gacagaaacc ttagctaaaa cctataaaaa aatcggcgaa
    47941 gaaaccaagg ttattgaagc gactgaacct ctaaccattc tgttaatggg agtggacacc
    48001 ggaaatgttg aacgaactga aacttgggtc ggtagaagtg atagcatgat cttgatgaca
    48061 gtgaatccta aaacgaaaaa aacaacaatg atgagtttag agcgggatat tctgacgcgc
    48121 attgaatcag ggaatggtca ggctcatgaa gcgaaactga actcagcata tgcagatggt
    48181 ggagcagagc ttgctataga aaccattcaa aaaatgatga atatccatat tgatcgctat
    48241 gtgatggtca atatgagagg gttgcaaaaa ctagtggatg cagtaggagg tattacagtc
    48301 aataatatcc taggtttccc aatttctatc agtgaccaag aagaatttaa tactatttct
    48361 atcggtgttg gggagcaaca tattggggga gaagaagccc tagtctatgc acgaatgcgt
    48421 taccaagatc ctgaggggga ttatggtcgt caaaaacgtc aacgtgaagt tattcaaaaa
    48481 gtcatggaaa aagctctcag tttaaatagc gttggtcatt atcaagagat tctaaaagct
    48541 ttgagtgaca atatgcagac caatattgat ttgtctgcaa aaagtatccc taacttgcta
    48601 ggctataaag attcatttaa aaccattgaa actcagcagt tgcagggtga aggagagata
    48661 cttcaaggtg tttcttacca gattgtttcg agagcacata tgttggaaat gcaaaatata
    48721 ctccgacgtt ctttgggaca agaagaagtt actcagcttg aaaccaatgc ggttttattt
    48781 gaagatttat ttggcagagc acctgttggt gatgaagata attaactttt gatacaaata
    48841 aaaaaatcaa tcgtaggaaa attcctgcga ttttttcaaa aaaatacgaa tagataggta
    48901 ggaggaaaca tgaaagcaga aatcattgct gttggaacag agattttgac aggacagatt
    48961 gtcaacacca atgcccagtt tttgtcggaa aaactagctg agattggggt agatgtatat
    49021 tttcagacgg ctgtaggaga caatgaagtt cgtcccttgt ctttgcttga gattgccagt
    49081 caacgtagca gtctggtgat tttgacaggc ggtttggggc caactgagga cgacctaacc
    49141 aaacaaaccc tagctaaatt tttagggaaa gcattagtct ttgatcctca ggctcaggag
    49201 aagttggata tcttttttgc cctgcgacca gactatgccc gaacaccgaa taacgaaaga
    49261 caagctcaaa ttgtagaagg agcgattcca ctgccaaacg aaacaggact ggctgttgga
    49321 ggaaaattag aagtagacgg agtgacctat gtcgtccttc caggtccgcc aagtgaattg
    49381 aaacccatgg tcttaaacca acttctaccc aagttgatga cagggagcaa gctgtattcc
    49441 cgagttcttc gtttctttgg gattggcgag agccagctgg ttacgatttt ggctgattta
    49501 attgataatc agatcgatcc taccttggcc ccttatgcca agacaggaga agtcactcta
    49561 cgtctgtcaa caaaggctag cagtcaagaa gaggcgaatc aagtgctgga tatcttggaa
    49621 aatcaaatct tggactgcca gactttcgaa ggaatttctt tacgagactt ttgctatggt
    49681 tatggggaag aaactagttt agccagcatt gtggtagaag aactgaaaag gcaagggaaa
    49741 accatcgcgg ctgcagagag tttgacggca ggtcttttcc aagctaccgt ggcgaatttt
    49801 tctggagttt caagtatatt taagggtggt tttgtgacct atagcttgga ggaaaaatca
    49861 aggatgttgg atattcctgc caagaatttg gaagaacatg gtgtggtgtc tgaatttaca
    49921 gctcagaaga tggctgagca ggcacgaagc aagacccagt ctgattttgg aattagtttg
    49981 actggagtgg caggaccaga tagcctagaa ggacacccag ttgggacagt cttcataggc
    50041 ttggcgcaag atcaaggaac tgaggttatc aaggttaata ttggaggcag aagccgagca
    50101 gatgtacgtc acattgcggt tatgcatgcc tttaacctag ttcgcaaggc tttattaagt
    50161 gactaacttt tgatataata gtagataggt ctgaggatca ttagaatgta ggagaataga
    50221 atggcgaaaa aaccaaaaaa attagaagaa atttcaaaaa aatttggggc agaacgtgaa
    50281 aaggccttga atgacgctct taaattgatt gagaaagact ttggtaaagg atcaatcatg
    50341 cgtttgggtg aacgtgcgga gcaaaaggtg caagtgatga gctcaggttc tttagctctt
    50401 gacattgccc ttggctcagg tggttatcct aagggacgta tcatcgaaat ctatggccca
    50461 gagtcatctg gtaagacaac ggttgccctt catgcagttg cacaagcgca aaaagaaggt
    50521 gggattgctg cctttatcga tgcggaacat gcccttgatc cagcttatgc tgcggccctt
    50581 ggtgtcaata ttgacgaatt gctcttgtct caaccagact caggagagca aggtcttgaa
    50641 attgcgggaa aattgattga ctcaggtgca gttgatcttg tcgtagtcga ctcagttgct
    50701 gcccttgttc ctcgtgcgga aattgatgga gatatcggag atagccatgt tggtttgcag
    50761 gctcgtatga tgagccaggc catgcgtaaa cttggcgcct ctatcaataa aaccaaaaca
    50821 attgccattt ttatcaacca attgcgtgaa aaagttggag tgatgtttgg aaatccagaa
    50881 acaacaccgg gcggacgtgc tttgaaattc tatgcttcag tccgcttgga tgttcgtggt
    50941 aatacacaaa ttaagggaac tggtgaccaa aaagaaacca atgtcggtaa agaaactaag
    51001 attaaggttg taaaaaataa ggtagctcca ccgtttaagg aagccgtagt tgaaattatg
    51061 tacggagaag gaatttctaa gactggtgag cttttgaaga ttgcaagcga tttggatatt
    51121 atcaaaaaag caggggcttg gtattcttac aaagatgaaa aaattgggca aggttctgag
    51181 aatgctaaga aatacttggc agagcaccca gaaatctttg atgaaattga taagcaagtc
    51241 cgttctaaat ttggcttgat tgatggagaa gaagcttcag aacaagatac tgaaaacaaa
    51301 aaagatgagc caaagaaaga agaagcagtg aatgaagaag ttacgcttga cttaggcgat
    51361 gaacttgaaa tcgaaattga agaataagct gttaaagcag tggagaaatc cgctactttt
    51421 tcgatttttg attcaagttt ttagattata tatagtagct tgaaataaga tatgaacaac
    51481 tttattagga aagtcaaatt aatttctaga aatgttttag cagctacagc gtactattcc
    51541 aaactcaacc aattataata gatcgaaact agaatagtac acatctactt ctaaaacatt
    51601 gttaaaaatc gatttgactg tccttatttc attccgctat atatagtttg ctgtttcttg
    51661 tcgctcctct ggaaagctga tataatagct ttatgaataa aaaacgaaca gtggacctga
    51721 tacatggtcc gattcttccc tcgctcttaa gcttcgcctt cccaatcttg ctatcaaata
    51781 tttttcaaca gctctataac actgctgatg tcttgattgt tggacgattt cttggtcaag
    51841 aatccttggc tgcagtagga gcgacgacag cgatttttga cctgattgta ggttttacac
    51901 ttggtgttgg caatggcatg gggattgtca ttgctcgtta ttatggggct cggaatttca
    51961 ctaaaatcaa ggaagcagta gcagctactt ggattttagg tgctcttttg agcattctag
    52021 ttatgttgct gggctttctt ggcttgtatc ctctcttgca atacttagat actcctgcag
    52081 aaattcttcc tcaatcttat caatatattt ctatgattgt gacctgtgta ggtgtcagct
    52141 ttgcttataa tctttttgca ggcttgttgc ggtctattgg tgacagtcta gcagccctgg
    52201 gatttctgat tttctctgcc ttggttaatg tggttctgga tctctatttt attacgcaat
    52261 tgcatctggg agttcaatcc gcaggacttg ctaccattat ttcgcaaggt ttatcagcgg
    52321 ttctctgctt ttattatatt cgtaaaagtg tgccagaact cttgccacag tttaaacatt
    52381 tcaaatggga caaaagcttg tacgcggatc tcttggagca aggtttggct atgggcttga
    52441 tgagttcaat tgtatctatc ggcagtgtga ttttacagtt ttctgttaat acatttggtg
    52501 cagtgattat tagtgcccag acggcagctc gacgcattat gacctttgcc cttcttccta
    52561 tgaccgctat ttctgcatca atgacgacct ttgcttctca gagtctagga gctaagcgac
    52621 ctgaccgcat tgttcaaggt cttcgaatcg gcagtcgttt aagtatatcc tgggcagttt
    52681 ttgtttgtat tttcctcttt tttgccagtc cagccttggt ttccttcttg gctagttcga
    52741 cagatggtta cttgatagaa aatggaagtc tctatctgca aatcagttca gccttttatc
    52801 ccattttgag cctcttgttg atttatcgca attgcttgca gggcttgggg cagaagatcc
    52861 ttcctctagt ttctagcttt attgaactaa tcggaaaaat cgtttttgtg gttttgatta
    52921 ttccttgggc aggatataag ggtgttatcc tttgtgaacc tcttatctgg gttgccatga
    52981 cagttcaact gtacttctca ctattccgtc atcccttgat aaaagaagga aaggcaattt
    53041 tggcaaccaa agtgcaatcc tagttggatt tactgaataa aattcatttc ctctagtgaa
    53101 catcgaaaaa acttgtgttc tcttctttag tttggtgttg aaaatagttt aacagacttt
    53161 tgacttcttt tatatgatat aataaagtat agtatttatg aaaaggacat atagagactg
    53221 taaaaatata cttttgaaaa gctttttagt ctggggtgtt attgtagata gaatgcagac
    53281 cttgtcagtc ctatttacag tgtcaaaata gtgcgttttg aggttctatc tacaagccta
    53341 atcgtgacta agattgtctt ctttgtaagg tagaaataaa ggagtttctg gttctggatt
    53401 gtaaaaaatg agttgtttta attgataagg agtagaatat ggaaattaat gtgagtaaat
    53461 taagaacaga tttgcctcaa gtcggcgtgc aaccatatag gcaagtacac gcacactcaa
    53521 ctgggaatcc gcattcaacc gtacagaatg aagcggacta tcactggcgg aaagacccag
    53581 aattaggttt tttctcgcac attgttggga acggttgcat catgcaggta ggacctgttg
    53641 ataatggtgc ctgggacgtt gggggcggtt ggaatgctga gacctatgca gcggttgaac
    53701 tgattgaaag ccattcaacc aaagaagagt tcatgacgga ctaccgcctt tatatcgaac
    53761 tcttacgcaa tctagcagat gaagcaggtt tgccgaaaac gcttgataca gggagtttag
    53821 ctggaattaa aacgcacgag tattgcacga ataaccaacc aaacaaccac tcagaccacg
    53881 ttgaccctta tccatatctt gctaaatggg gcattagccg tgagcagttt aagcatgata
    53941 ttgagaacgg cttgacgatt gaaacaggct ggcagaagaa tgacactggc tactggtacg
    54001 tacattcaga cggctcttat ccaaaagaca agtttgagaa aatcaatggc acttggtact
    54061 actttgacag ttcaggctat atgcttgcag accgctggag gaagcacaca gacggcaact
    54121 ggtactggtt cgacaactca ggcgaaatgg ctacaggctg gaagaaaatc gctgataagt
    54181 ggtactattt caacgaagaa ggtgccatga agacaggctg ggtcaagtac aaggacactt
    54241 ggtactactt agacgctaaa gaaggcgcca tggtatcaaa tgcctttatc cagtcagcgg
    54301 acggaacagg ctggtactac ctcaaaccag acggaacact ggcagacaag ccagaattca
    54361 cagtagagcc agatggcttg attacagtaa aataataatg gaatatcttt caaatcagaa
    54421 cagcgcatat tattaggtct tgaaaaagct taatagtatg cgttttcttg tggagatatt
    54481 tccttcaatt tttgttacta tattaaacaa aaatcaaaaa gcaaactaga aagttatgct
    54541 caaataaaat ctaaatttga caatgtaaac cgagtcggat agctttaagt actgttttga
    54601 ggttgaagat acgatttttg ataggaactc atcaatttta gatttttaag cagcatcaat
    54661 aaattgcttc cttgttttgt cataattttt ttatttaaaa aattatgaca aaagtgtgct
    54721 attcttttta tgagaggtgt atgaatatga taaatgtatg tgataaatgt atgtgatgtt
    54781 ggaaaaagaa taaaagaact tagaatatct tcaaatctta ctcaagataa gattgctgaa
    54841 tatttgtctt tatatttgtc tttgaatcaa agcatgattg ccaaaatgga aaaaggtgaa
    54901 aggaatatca cgaatggatt taagtaataa agcttcaaat cttagaaaaa agttgggagc
    54961 tgatggtgaa gcgccgatag atatttttaa attggtacaa aagatagaaa atttgacgct
    55021 ggtattttat ggactcggaa agaatattag cggagtctgt tataaaggaa ctcagttcag
    55081 tctcattgca gtcaattcag acatgtcatt aggaaggtaa agattttctt tagcacatga
    55141 actgtatcat ctttattatg atgaggtgaa gaagagttca gtcagtctta tcttgattgg
    55201 tgaaggagat gaaactgaaa gaaaagcgga tcagcttgct tcttattttt taattttccc
    55261 atcttcactg tataggatgg ttgaggaaat cagagaaaat gccaatagaa ctcatcttga
    55321 agtagaagat attataaaat tgggtcagtt ttatggtatc agtcataaag ctatgttata
    55381 tagattgagg aatgatggat accttgatgc agaagaaatt aaaaatatgg atattagtgt
    55441 tatagagaca gcttcaagat taggctatga tacaagttta tatcgtcctt tgtcagaaag
    55501 taaaaaggaa atggtattag gataatatat taattcaact gaacaacttt tagaaaataa
    55561 cagaatttcg caagggaagt atgaggaact gttactagat gctttcagat atgatattgt
    55621 atatgggcta gatgaagagg ggggagttgt cgtttgacta gtcgtgtatt tattgatgca
    55681 gattgtattt cagtattttt atgggttggc actgaacatc ttttagaaaa gctctattcg
    55741 ggtaaaattg ttattccaca agaggtgtat gatgaaatca atatacctac aattccccat
    55801 ttaaaatcta ggatagatca gttggtagct aagggttcag ctgagattgt gagcatagac
    55861 attggaactg aagaatacgc attatataga gatttaacaa gaaatcatga taataacaag
    55921 attattggta agggagaagc ggcttctatt tccttagcga aaaagcataa tgggatatta
    55981 ggaagtaata acctaagaga tgttaaatca tatgtagaag aattttcttt agaacatatg
    56041 acaacaggag atatactgat tgaagcgttt aaagcgtaat ttattactga acaagagggc
    56101 aatcatatct ggaataatat gcttaaaaag agaaggaaaa ttggtgcaaa ttcattttca
    56161 gactatcttc gtggaagtat tcatcaaaat agacaaaaat aaatttggat aaatcgaact
    56221 cactattcag gaggcatatg agcaattcga aaaagaaaaa tgtcaaattg agcctatagg
    56281 agtagaagtg aaatagtaag tcctgcatag tggatgagag aaaagttctc cttgaagttt
    56341 tcctgaacta tcagtcgcat gtcaaacgat atgtagggta atgtgagagg ggatagcgag
    56401 tagtttttgg ttattttatc aaaaaactta tattttatta tactgaatga taaaatataa
    56461 taaaaatgat agaataagga aaaaacatga atgtcaaaaa gataatgtca atttttcaat
    56521 ccttttatgt tgatgtcagt attgaggaac tgactttgac tttaccaatc agttttgtaa
    56581 aaaggtttga gtatactcaa atgacttttc ataaggaatc atttttattg attaaagaaa
    56641 agagaagggg gagtttgagt tcatttgtta ctcaggctcg cactatgggt gaaaaagcca
    56701 atatggatgt tgttttgctg ttttcgaagt tatcagacag tgaaaaaaag caattacttc
    56761 aagctagagt tccgtttgta gactttaagg gaaacctctt cttccctcca ttgggactag
    56821 tactgaatgc gaatgatact gaagtcccta aggaattaac acctagcgaa caattaacgt
    56881 ggattgcctt tttattgaca aaaggtcaaa aagtagtaga tgttgatttg ctttcacaag
    56941 tcactggact tccaaactca acaatttata ggtgtttgag gacttttaaa gctttatatt
    57001 ggttaaacaa gcaaaataag ctttacacat atacggtgtc aaagaaagaa ttattcttaa
    57061 aatccgtgtc atgtttattt aatcccatca aaaaacggat tttattgcca gatggcgata
    57121 taaagcagat aaaatctgtt tctaatcttc tatatggtgg tgcttatgct ttgtcgcatt
    57181 caactttttt agctgaaacg gatgaaaata ttagctatgt catatggcag agaaaattca
    57241 atcagttatc cttgccattt tctcagcata ttttaaaagg aaagatgcta gagatatgga
    57301 aatatcgtcc ttttgtatct gagttttgga atgattttaa aaataatcat gataaacaat
    57361 ttgtagatcc gatttctctt tatttgacct taaaagatga tgatgaccca cgtatagagg
    57421 aagagagtga agcactagaa aatatgatat tacagtatct gggagaagat gatgccagct
    57481 aatacgaaag ttatttttca agaaacgttt gcggattttc agaactatta tgttctgatt
    57541 gggggaactg ctacctctat cgtattggat tcgcaaggat ttaaaagtcg cacaacaaaa
    57601 gattatgata tggtcatcat tgatgaagta aaaaataagg aattttatac taccttgaat
    57661 cattttttag aattgggaga gtatcaagga agtcagaaag atgagaaagc gcagcttttt
    57721 cgatttacaa caactaatcc tgagtttcct tctatgattg aactatttag tatcttacca
    57781 gaatatccat taaagaagga cggtcgagaa attcccttac attttgacca agatgctagt
    57841 ttatcagcct tattattgaa tgaagattat tataatatat tggtgcatga aaaagaaacc
    57901 attcaggggt atttggtatt gagtaattgt ggtc
//
LOCUS       DAVKWZ010000006        51656 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000006 DAVKWZ010000000
VERSION     DAVKWZ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 51656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51656
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.006"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            110..388
                     /locus_tag="VW854_000384"
     CDS             110..388
                     /locus_tag="VW854_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000397732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261680.1"
                     /translation="MEFWNDTIQTVKEWRPVNYSVAEDEDFEAVKKKAIRNFQDYLKL
                     LDENTEEGYQEVIRLFTFSKFYGEELCNDEELNKLSRDIRGRLKKFRS"
     gene            650..1927
                     /locus_tag="VW854_000385"
     CDS             650..1927
                     /locus_tag="VW854_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HEV0261681.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     IKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATPEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKFLRRQFSKKNLTDEEETMIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            2003..3487
                     /locus_tag="VW854_000386"
     CDS             2003..3487
                     /locus_tag="VW854_000386"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEV0261682.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLNF
                     NKLKDASYQEVAKLVLSAFLDDFTAEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGDKINFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNDAVKTAELMNALNSQGQYELTNFDAEILDLFAAEYATEEETAAEIKRVYESDS
                     YIEDPHTAIASAVYRKYQATTGDATKTVIASTASPYKFPVVAVEALTGKVGLTDFEAL
                     AQLHEISGVAVPPAVDGLETAPVRHKTIVAAADMQVAVEAYLGL"
     gene            3548..4828
                     /locus_tag="VW854_000387"
     CDS             3548..4828
                     /locus_tag="VW854_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV0261683.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTILSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMNLKG"
     gene            4832..5452
                     /locus_tag="VW854_000388"
     CDS             4832..5452
                     /locus_tag="VW854_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV0261684.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFALFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            5618..6760
                     /locus_tag="VW854_000389"
     CDS             5618..6760
                     /locus_tag="VW854_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV0261685.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEAPKEVASSSEVTKTV
                     IASEEVAPSTGTSVPEEQTAETSSAVAEEAPQETTPGEKQETQASPQAASAVEATTTS
                     SEAKEVASSNGATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAA
                     FKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVPESSELGDKIAEYAIQNMASRGI
                     SYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            7166..7504
                     /locus_tag="VW854_000390"
     CDS             7166..7504
                     /locus_tag="VW854_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEV0261686.1"
                     /translation="MTRYQILLFLLEHSPCNQMAVQERLKIDQAALTRHFKILETEGL
                     VERHRNPENQREVLVEAAKYAKEQLVVNPPLQHIKVKEEMESILTEFERTELSRLLNK
                     LVLGIENIEI"
     gene            7515..7873
                     /locus_tag="VW854_000391"
                     /pseudo
     CDS             7515..7873
                     /locus_tag="VW854_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861952.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
     gene            7980..8597
                     /gene="pcp"
                     /locus_tag="VW854_000392"
                     /pseudo
     CDS             7980..8597
                     /gene="pcp"
                     /locus_tag="VW854_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
     gene            complement(8625..9482)
                     /locus_tag="VW854_000393"
     CDS             complement(8625..9482)
                     /locus_tag="VW854_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEV0261687.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSSFLNP
                     LSRIHGTVLEQPFFSILGQILQAYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQRFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            9612..10754
                     /gene="tgt"
                     /locus_tag="VW854_000394"
     CDS             9612..10754
                     /gene="tgt"
                     /locus_tag="VW854_000394"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEV0261688.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            10852..12669
                     /locus_tag="VW854_000395"
     CDS             10852..12669
                     /locus_tag="VW854_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEV0261689.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAVAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQESKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPIVQTLRPLPYIHAILAAGTGILTIIVCTVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            12822..13973
                     /gene="nagA"
                     /locus_tag="VW854_000396"
     CDS             12822..13973
                     /gene="nagA"
                     /locus_tag="VW854_000396"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEV0261690.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            14136..15194
                     /locus_tag="VW854_000397"
     CDS             14136..15194
                     /locus_tag="VW854_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEV0261691.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPATFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            15345..15710
                     /locus_tag="VW854_000398"
     CDS             15345..15710
                     /locus_tag="VW854_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEV0261692.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            15786..16727
                     /locus_tag="VW854_000399"
     CDS             15786..16727
                     /locus_tag="VW854_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEV0261693.1"
                     /translation="MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDHKIASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAS
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            16675..17691
                     /locus_tag="VW854_000400"
     CDS             16675..17691
                     /locus_tag="VW854_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEV0261694.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSALLDKQCVTQMRVGLSQGKSFSEMMESLGCSSAIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEVGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            17693..17755
                     /locus_tag="VW854_000401"
                     /pseudo
     CDS             17693..17755
                     /locus_tag="VW854_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGC"
     gene            complement(17773..19218)
                     /locus_tag="VW854_000402"
     CDS             complement(17773..19218)
                     /locus_tag="VW854_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV0261695.1"
                     /translation="MKTMNKVAIYVRVSTTSQVEEGYSIDEQKAKLSSYCDIKDWNVY
                     KIYTDGGFSGSNTDRPALESLIKDAKKRKFDTVLVYKLDRLSRSQKDTLHLIEDVFIK
                     NGIEFLSLQENFDTSTPFGKAMIGLLSVFAQLEREQIKERMQLGKIGRAKAGKSMMWA
                     KTSYGYNYHKETGTVTINPAQALAVKFIFESYLRGRSITKLRDDLNEKYPKHVPWSYR
                     AVRTILDNPVYCGFNQYKGEIYPGNHEPIISKEEYDKTQSELKIRQRTAAENVNPRPF
                     QAKYILSGIAQCGYCGAPLKIMLGVKRKDGSRLKKYECHQRHPRTLRGVTTYNDNKKC
                     DSGFYYKDKLEASVLKEISKLQDDADYLDKIFSGDNAETIDRESYKKQIEELSKKLSR
                     LNDLYIDDRITLEELQSKSAEFISMRATLETELENDSALRKNKRKADMRKLLNAEKVF
                     SMDYEKQKVLVRGLINKVQVTAEDIVIKWKI"
     gene            complement(19497..20261)
                     /locus_tag="VW854_000403"
     CDS             complement(19497..20261)
                     /locus_tag="VW854_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEV0261696.1"
                     /translation="MSKENPYFEQTKQNYIEVEKLYKLGKAKHTSSKYRFLAPAVKRQ
                     SEQFLFEAKTQKRKYWKFNRGSLIFVEFGVNIGGELSNNHWAIVLDKVDSPYKKTLTV
                     IPLTSKNQIDTVLIDEIIAEYPSILLDEYIEKLHKELFAYLKYLDSNNAITEAALSDV
                     YQAYTEQFSNEIIQPKIVDDDNLKRTQSEITDIIELTQYYKKYIKRSYAKCNNLQTIS
                     KDRILKKNRLDPIGKMKVSDNTLDKINEKLKELYLF"
     gene            complement(20263..21066)
                     /locus_tag="VW854_000404"
     CDS             complement(20263..21066)
                     /locus_tag="VW854_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV0261697.1"
                     /translation="MRSSNPSERLKQLMSERNLKQVEILEKSLPYQKELGIKMGKSTL
                     SQYVNGVQSPDQHRIYLLSKTLEVSEPWLMGYDVEKKRIPDSERNSISNEQPEILPIY
                     NKLEEPRQEKVLDFAKKQLEEQKKSKASSIFDKSQDGDDYITDYVEGLVAAGHGTFQE
                     DNLHMEVRLRAEDVPEDYNTIAKVAGDSMEPMIEDNDLLFIKVTNQVDINDIGIFQIN
                     GKNFVKKLKRDYNGGWYLQSLNNSYEEIHLTENDDIRTIGEVVSVYREK"
     gene            21236..21472
                     /locus_tag="VW854_000405"
     CDS             21236..21472
                     /locus_tag="VW854_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF739 family protein"
                     /protein_id="HEV0261698.1"
                     /translation="MSNDYSKLAGKIVEKYGTQYNFAQAMGLSERSISLKMNNRVPWK
                     DFEMAKASELLDIDVNQLHEYFFTPKVHVREQIA"
     gene            21485..22210
                     /locus_tag="VW854_000406"
     CDS             21485..22210
                     /locus_tag="VW854_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HEV0261699.1"
                     /translation="MNELINVTLNENQEPVVSARQLHQTLEVKKRFSAWFKANSKLFI
                     ENEDFTSVQTGTVVNNGAIKPLQDYALTLDMAKQLAMMSRTIKGAEIRQYFIQVEKDF
                     NSPEKIMARALLMADQKVHKLEAQIEADRPKVLFADAVSASKSSCLIGELAKILKQNG
                     IDIGQNKLFQWLRSNGYLISRRGESWNQPTQKSMQLGLFELKKTNINHADGHTTVSTT
                     TKVTGKGQQYFINKFLNQEYLTG"
     gene            22283..22441
                     /locus_tag="VW854_000407"
     CDS             22283..22441
                     /locus_tag="VW854_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261700.1"
                     /translation="MKTVKKEWEPRIVNIMADGSQVDDLTGYVIPAGHSYYDIILGMH
                     KRELQKGA"
     gene            22451..22804
                     /locus_tag="VW854_000408"
     CDS             22451..22804
                     /locus_tag="VW854_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001163840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV0261701.1"
                     /translation="MQYIIRNTNENYTQMNNHSAQNSELSLQAKGLLLVLMSNKDTWR
                     PYIDELSKRSRNGRDAHRAAFDELKEAGYIRIYRKSFGRGKGIQNFPLVQDVPISDSY
                     WEYWVSNLEKELSTE"
     gene            23045..23569
                     /locus_tag="VW854_000409"
     CDS             23045..23569
                     /locus_tag="VW854_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261702.1"
                     /translation="MKKIATAMNVSVSDLFTQDTPIKKNRHSTPVNPKIYKEFIDNVN
                     QYQRLTGATYEKISNIIGKSNSYIYDVIDKQRKSTLSIKSNASLTKGSMILRQEIEKI
                     ESGKNRIPSKLTYQTIDRDSEVVFRFNGLIKSVNDLSDKELQIIVSIFDALKIPAKIS
                     KIEIRETNVFGGGK"
     gene            23621..24073
                     /locus_tag="VW854_000410"
     CDS             23621..24073
                     /locus_tag="VW854_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261703.1"
                     /translation="MPNWAEGTLKLRGRRENVASALKEMLLGNKGATLEEEYDGTLLI
                     FKNEYDYFYINGTRRAFISSKDIEIWLDDDFVIIELEDFEQAWAALADNYTEISSKFD
                     VDIKIFTFEMGMEFTQEIEISKGEIIKNIVNENFTNYSWDVPFSRLGG"
     gene            24079..24783
                     /locus_tag="VW854_000411"
     CDS             24079..24783
                     /locus_tag="VW854_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HEV0261704.1"
                     /translation="MADLTFAELQRKMQIEKQTKQGVKYPFRTAEDINNKFKSLDSGW
                     SVSFPEDDIIQKGDKLYYKAVAVAKRESDGTIEKAIGWAREEDVPIFHTQKGDVKQMQ
                     DPQWTGAVGSYARKYALQGLFAIGGEDVDEYPVEESQEQGQNNQQQQQQGNQQAQGQN
                     QVRYIDNIQYQEINDLINDIAKIKGMPFDTLANYVLSEKLKGLQDFHRVQVGDYEVLK
                     NYLTEQLAKAKAKRGN"
     gene            24786..25778
                     /locus_tag="VW854_000412"
     CDS             24786..25778
                     /locus_tag="VW854_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1351 domain-containing protein"
                     /protein_id="HEV0261705.1"
                     /translation="MVKDVTNSLTEIKVDFQPAVINVDREAIEVQVATAIAQYSGREV
                     TVDNYKEVYEERTRFNKLIGGLDTQRKDFNRQINEPAKDFDKWVKEKVIKPIEAVTDA
                     MSAGLNAIDEHERLMRVDVVRATFEDKCMVAGIEKSTFADKYDEYSLKKYFKTGKYEL
                     KKTTLDEMDGLVLSEFDALEEYKANKQAIQEQAQEYDLPADSYIRHLEDGKSLVDILK
                     MMKTDRDAEIARKEQKEAQEKAKAERLEEIAQSAKKNANANIKAYDAETGEILEQGTI
                     TPEPQNNAREVAKFEPSEPLVKLVRLELHGGLEQWENTQEYFEDNFIGFETLED"
     gene            25800..25976
                     /locus_tag="VW854_000413"
     CDS             25800..25976
                     /locus_tag="VW854_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261706.1"
                     /translation="MRCFYVSGKIADLDLGSEINAENSFMAAIEFVKRYTDLLKFDSN
                     EIKVSEVEEVQNDK"
     gene            25966..26511
                     /gene="ssb"
                     /locus_tag="VW854_000414"
     CDS             25966..26511
                     /gene="ssb"
                     /locus_tag="VW854_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV0261707.1"
                     /translation="MINNVVLVGRLTRDAELRYTQSNIAVATFTLAVNRPFKNEAGER
                     EADFINCVIWRQSAENLANWAKKGSLIGVTGVIQTRSYDNQQGQRVYVTEVVASNFQL
                     LESRNSQQNNQGHQDHHGGYQQQGYSNQGSSFQNGNSYGQQGSFVEGNTTNLVPDFTR
                     DNNPFGRPTNPLDISDDDLPF"
     gene            26537..27676
                     /locus_tag="VW854_000415"
     CDS             26537..27676
                     /locus_tag="VW854_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEV0261708.1"
                     /translation="MKFLDLFAGIGGFRIGMESAGHECIGFCEIDKFARASYKAIHDT
                     KGEIELHDITTVSDDTIRGIGSVDIICGGFPCQAFSIAGNRRGFEDTRGTLFFEIARF
                     ASILRPKYLFLENVKGLLNHENGVTFETIISTLDELGYNVEWQVLNSKNFGVPQNRER
                     VFIIGHSRKRGTRRVFPIGRANREFDFEPKIKIVGNTKNPNGTSQGTGSVVYDSNGLV
                     GTLCARDYKEPKQVAIPVLTPDRVNKRQNGRRLKKNGEPMFTLTAQDRHGILIKEATK
                     KGYAEATVGDSVNLSHPNSKTRRGRVGNQIANTLLTGESQGVVEPDFRIRKLTPRECW
                     RLQGFPDWAFDKAQEVNSNSQLYKQAGNSVTVNVISAIAQGLGGN"
     gene            27711..27914
                     /locus_tag="VW854_000416"
     CDS             27711..27914
                     /locus_tag="VW854_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261709.1"
                     /translation="MTKIEIVMVLTTLMSITWAAIVTIHTMQAIKKHKAKVDYYQKPQ
                     VQCEIARHVLKNKWYSDGGEVFR"
     gene            27911..28027
                     /locus_tag="VW854_000417"
                     /pseudo
     CDS             27911..28027
                     /locus_tag="VW854_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039279.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            28050..28799
                     /locus_tag="VW854_000418"
     CDS             28050..28799
                     /locus_tag="VW854_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEV0261710.1"
                     /translation="MKFELINDHFENAKRYNIPRAQLIIADIPYNLGNNAYASDPRWY
                     ENGDNKNGESKLAGKSFFDTDNDFKINNFFDFCSRLLKKEPKEKGKAPAMIVFHAWQQ
                     RDMIIECGKKHGFNNAYPLYFTKKSSPQALKANMKIVGAVEEATVLYRDKLPKFNNGG
                     AMILNHAPWEKDRSYPVIHPTQKPIPVLKRLIEIFTDEGDVVIDPVAGSGSTLRAAIE
                     MNRSAYGFEIKKDFYKAAQEKMLSSFQISLI"
     gene            28814..29248
                     /locus_tag="VW854_000419"
     CDS             28814..29248
                     /locus_tag="VW854_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261711.1"
                     /translation="MDKKLIGLDLTHIADGGLQEKLDKELEKVFDNILDLNTDAKAKR
                     KVTITLTMSANEERTVVDTTMEVKSKFAPQNGVATTILIGRDFDTGQVHANELKSTVP
                     GQMYFDENGEILTDIGQPVAEIEQQAETKPDIIDFNKKKVGN"
     gene            29250..29987
                     /locus_tag="VW854_000420"
     CDS             29250..29987
                     /locus_tag="VW854_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261712.1"
                     /translation="MTTENLKSVLEYVVELNEHGLEILTAADGTEYYDANKFNLKELD
                     PKHYPKTLELSTLTSLVDYLKTDLNNLKNQRLIVAVEKNDEVCVWSENDEIEHRTLLV
                     DVKARIPELSFGRFLSLEQFNIMLQSNFIDDNDRGTLLEFASALKIENGAEIEDNGVS
                     QVATVKTGVASLAKGKAPNPVTLRPYRTFSEVEQPASLFVFRIDKQANMALFEADGKH
                     WVADAVGNIAAYLKEQLADQKHITVLA"
     gene            30004..30216
                     /locus_tag="VW854_000421"
     CDS             30004..30216
                     /locus_tag="VW854_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261713.1"
                     /translation="MSDYKQRMIEEYKQLKERTNKLSLMISNYYVGTLDFKLKCPIEL
                     LETQHYTMCAYLKILEQRAEIENIEF"
     gene            30227..30628
                     /locus_tag="VW854_000422"
     CDS             30227..30628
                     /locus_tag="VW854_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261714.1"
                     /translation="MKFEFSLPRNTKLKSLNMVINSNDRQHQTDKAKVTKRIRAFAYW
                     HTSMNKDKGRAAFSPSNPCEVTVTIYSPTKSKLDPPNLYPTVKAIIDGMTDAGIWTDD
                     NHKVIKKLSFVYGGLSEEKGHYRLEFDIEEV"
     gene            30630..30836
                     /locus_tag="VW854_000423"
     CDS             30630..30836
                     /locus_tag="VW854_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261715.1"
                     /translation="MIEVNIKFDNFEAHGFYQDDTKLGKIRDAIISQMNNGHVVVLGE
                     DRGILLNPKVIKSVQFKVVEDNQI"
     gene            30885..31202
                     /locus_tag="VW854_000424"
     CDS             30885..31202
                     /locus_tag="VW854_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261716.1"
                     /translation="MMEELKQKVNEVYNWTVEDGKPQPPKQDLPQAVKDRADYFWEMA
                     EDGMTFMGAMECIFADEKPTDYDLGATKDWLPKSKEFDDWVGYSPSMAQVVIAVYLIY
                     RGN"
     gene            31204..31707
                     /locus_tag="VW854_000425"
     CDS             31204..31707
                     /locus_tag="VW854_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEV0261717.1"
                     /translation="MNVQRLIEKYKKLEGVWDAEGAELARQIFLEDLKQLDEQKPVKV
                     PQCVAEYIEFKKKNNFHVYGAMRVIEDHYDKKVPDWFYENNIEKFCLAWLNGYEVEKE
                     KRYFVKIKGNIKGNMLVYGELLKRYFFTKNFSLDNVIYSHTRKELEDANFGWVFDCPG
                     IEIEEVE"
     gene            31708..31920
                     /locus_tag="VW854_000426"
     CDS             31708..31920
                     /locus_tag="VW854_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261718.1"
                     /translation="MTKFVKIQSCYRGHTDDELINIDDISRLCLGPNILFLRTPYNLG
                     EHHISITQNSVDKLLKVLDIIGEDGK"
     gene            31917..32288
                     /locus_tag="VW854_000427"
     CDS             31917..32288
                     /locus_tag="VW854_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261719.1"
                     /translation="MRPCKYPFLGRRKKQETPSPIFSARPIFDEVPIVEEVKVEFGVE
                     ASMGRIYPETLIHLDISGYGNRVHSVHRFPGILLSVGESIQLKMLFYKRLRNFTTDRF
                     LTFRESDWKFFIRDLVNEFKH"
     gene            32361..32765
                     /locus_tag="VW854_000428"
     CDS             32361..32765
                     /locus_tag="VW854_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEV0261720.1"
                     /translation="MNKAKELLKELQDLDMDIQSRIDEINELEAGLLSSPKWSEVKVQ
                     GGQTRKVDDVYTQLVVMKEAIEQDTKEVINRKLELGRMINRLKNPKYRAVLRMTYINK
                     GTADSVCYDLNMSRTTYYRLKNEAVLALEEVI"
     gene            32846..32928
                     /locus_tag="VW854_000429"
     tRNA            32846..32928
                     /locus_tag="VW854_000429"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32877..32879,aa:Tyr,seq:gta)
     gene            34012..34524
                     /locus_tag="VW854_000430"
     CDS             34012..34524
                     /locus_tag="VW854_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEV0261721.1"
                     /translation="MAFFDCLKGGDGKLNERQRRFADEYIISGNAYQSALRAGYSEKY
                     AKARSSELLDNVGISDYIKNRMEELQDEKILTQKQILVMLSEIASGQAKETIVVTTKV
                     AELMTDPVTGKSVKVYNEIPQLVEYPTKNSDRNKALELLGKRHKMWTDKVEADVSGTV
                     VFANESDIPD"
     gene            34502..35800
                     /locus_tag="VW854_000431"
     CDS             34502..35800
                     /locus_tag="VW854_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEV0261722.1"
                     /translation="MSQTYQIKQSDIVIDLPKTVGAGYGQFWRSRNLYRVVKGSRGSK
                     KSKTTALNYVIRLLKYPWANLLVIRRYSNTNKQSTYTDFKWACNVLGVTHLFKFNESL
                     PEITIKATGQKILFRGLDDELKITSITVDVGSLCWAWFEEAYQIETEDKFSTVVESIR
                     GSLDVPDFFKQITVTFNPWNERHWLKRVFFDEETRRADTFATTTTYKCNEWLDEVDIK
                     RYEDLYHTNPRRARIVCDGEWGVAEGLIYENVTVKDFDKDELLRDSANKLCIGLDFGF
                     THDPTALCCSLINDTTKEIYVFDEAYKVGLITKEVAKMIKDKGYHRSQIIADSAESRL
                     IEELRSEHGISRIKESRKGKDSIMAGVSKLQGYTIYVHPDCKNIMDEFYSYCYQRDKE
                     GNWLNKPEDKNNHLMDALRYSLQCIEGGKATVRRRSDYGL"
     gene            35812..37281
                     /locus_tag="VW854_000432"
     CDS             35812..37281
                     /locus_tag="VW854_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEV0261723.1"
                     /translation="MYQYLTYPRDGYDEGSLKKDLIYKLITIHSTEGSHLKKLKSYYL
                     GEHAILKHTRRNVNAPNYKTVANHAKDIADTATGYFMGNPIKYNNTADGDIDELLTAF
                     DGAEIDQVDAQNALNMAIYGRAYEYIYAKEGMAELDSTSIDPENTFMVYDDSIERKPL
                     FAVYYYEVKDDTKDTTKHQAEVFTENLHYHMVLRSTDSGTTQSEEATPHNLGQIPIIE
                     YRNNHFAIGDYEQQISLIDAYNSLMGNRVNDKEQAVESILVLYGTQLADTPEDAKVAM
                     KILSEEGLLELPGDSARAEFLKNTLDESATEILRTALKEDIYTFSHVPNLTDENFAGN
                     TSGVAMEFKLMGLEMITKTKEANYKRGLRQRIAIFAHYLGMKQIALESHSIVPQFSRG
                     LPKNLLEISQIVNNLEGKVTNRQLISLLPFVEDPDAELEALEEEKKKNMEDMPMFNQD
                     NTKPEDEVEDEESGVLGEEESQSDLPADGQGRKAGRSVR"
     gene            37190..38221
                     /locus_tag="VW854_000433"
                     /pseudo
     CDS             37190..38221
                     /locus_tag="VW854_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795723.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
     gene            38581..38808
                     /locus_tag="VW854_000434"
     CDS             38581..38808
                     /locus_tag="VW854_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HEV0261724.1"
                     /translation="MAILDDLQALYDNGWDASFNYNGQVCGIFPNSIYDIVVIIADNE
                     YRASSFDDLISLQIEGKTLPEIMNEVEVQYG"
     gene            38860..39105
                     /locus_tag="VW854_000435"
     CDS             38860..39105
                     /locus_tag="VW854_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261725.1"
                     /translation="MKYRKKPVVVEAVQWNGNNHKEVIDFAENKIWFDALGNIWIATL
                     EGDMVAKKGDYIIKGVQGEYYPCKPDIFAETYEKTEE"
     gene            39107..39358
                     /locus_tag="VW854_000436"
     CDS             39107..39358
                     /locus_tag="VW854_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261726.1"
                     /translation="MLKKAKQLASQEFSRLAGREIKEEDCFVVWFSKTLQNWKALVST
                     NAITSSEPCGDYAEITHNGDKNETYVDVYAKVSNRAIKD"
     gene            39585..40109
                     /locus_tag="VW854_000437"
     CDS             39585..40109
                     /locus_tag="VW854_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEV0261727.1"
                     /translation="MSEEINATVSTESTETVDTQENVETVQEEKHERTFTRAEIGKML
                     SAERSKWEAEQEAKENEAKKLAKMNADEKQKYQLDQREQELADREKAIARKELTAEAK
                     AMLSERDLPVELVNVVDLTNAETVSESITSIQKAWEESVQKGVSERMKGSAPIKNAQT
                     VQQEVTEKWRKDFL"
     gene            40132..41157
                     /locus_tag="VW854_000438"
     CDS             40132..41157
                     /locus_tag="VW854_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEV0261728.1"
                     /translation="MAFEELNTAESRKKHLGIIEDVLAVNSYSTPLVTSSDAVTLQGR
                     SFTVATGNTTELKDYKRNKDNEFDHVEVEEKVYTLDEEKYWGRFVDQLDERDSNGQVN
                     INYVIARQAAEVVAPYLDELRFGAALGNVSDNVAMGKTAGANNAYNAVLDVSEKLDEL
                     GITKERLLFVTPSFYKAIKSEIVRLPHGDADKKVLGKGYVGELDDYTVYKVPSKFLKG
                     VNALATAPGVVTSPVQVDNTKYNDNIPGRFGELVEQLLYTGAFVLEHFKKYIITIADS
                     KPAAKPSTQGKVVNRAKAWKTGTTYKEGDTVTHADKVYVAVKDISNSSTAPDTDTTNW
                     KEKTGKK"
     gene            41167..41355
                     /locus_tag="VW854_000439"
     CDS             41167..41355
                     /locus_tag="VW854_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261729.1"
                     /translation="MKFKIKQDFYDWESNVKRLAGEELEITEERYAELADNIASNGVA
                     ISDVLEEIFPKPEFLEED"
     gene            41357..41668
                     /locus_tag="VW854_000440"
     CDS             41357..41668
                     /locus_tag="VW854_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HEV0261730.1"
                     /translation="MSIELLKKLTGEEDTQLLMLLQTRATNLILSETNRTSLTPALSL
                     LIPEVAIELHNRSGAEGERSRTEGGIAVVYGENGLSTGLLQRIRMHRLARVAGHVFEA
                     E"
     gene            41649..41987
                     /locus_tag="VW854_000441"
     CDS             41649..41987
                     /locus_tag="VW854_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261731.1"
                     /translation="MFLKQSRLKPYPMRRFEKTVTEEGVAKEGYVKEAETIRLELWPA
                     SSKLQSELYGERVNDILNANANKSATIKVKDGVCIDSQTEVTHRVISKKVYTHHQVLE
                     LERVRATRGR"
     gene            41962..42477
                     /locus_tag="VW854_000442"
     CDS             41962..42477
                     /locus_tag="VW854_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEV0261732.1"
                     /translation="MSGLLGADRLIAKCRRLASKKTGEDIVLRAVHNATIKVVQADAR
                     RLAPARDGELITSIKTRAKMDGDKAIGEVYTNLKYAPYVEFGTGPIGQASHSGISPEV
                     SVTYKSSPWYVHEDQINVGPYHFQKIGEFYKMYGQPAQPYLYPALRDNQERVSKNISN
                     YVRRKIREQIK"
     gene            42474..42842
                     /locus_tag="VW854_000443"
     CDS             42474..42842
                     /locus_tag="VW854_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261733.1"
                     /translation="MINIKPVIYKELQKVADNVTDTYPSDWENFPVVIFLEEQNKPGD
                     WFDDQEQKSSIRYKVDIFDDTSTSELAVKINQIFESLGLRRTDCQDVPDPSHLRHKVM
                     RFEGVVDLDSELVFQFRMEN"
     gene            42845..43258
                     /locus_tag="VW854_000444"
     CDS             42845..43258
                     /locus_tag="VW854_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEV0261734.1"
                     /translation="MLANGIKLAFSETKGNYQNLVGLKEVPEFGIEPEKVENTTLADT
                     VKKYEFGIGDAGELEYKFAYNNSSATAPYRVLRKAADGKKKLYFEQTYPDGTKVNFEG
                     QVSVKLGGGGVNAVIEFTLKIALQSNLTFTDGVGG"
     gene            43263..43643
                     /locus_tag="VW854_000445"
     CDS             43263..43643
                     /locus_tag="VW854_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261735.1"
                     /translation="MALKYTTWKVTDEKELKLRLTSHQAATVEEKIGMNLLKIFMPEA
                     GEEFTLPPLKVMLLLVHGALQQYEHGYSFEDVYDLYDEYVDNGGDQTTFMTEVLMPLF
                     EVSGFTPRGSKDKKTSKKKMTVVK"
     gene            43682..44032
                     /locus_tag="VW854_000446"
     CDS             43682..44032
                     /locus_tag="VW854_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261736.1"
                     /translation="MFLDIGGKPLDFWDLTVLEIREMIESYNRVKIQERKEKIIDSYI
                     LSRMITNHVSLLLSNDAKIVELWEYAPELFVEEQQAVEQERQKQALLLHKERMREFAE
                     RHNRKRKEEVNGNS"
     gene            44019..47672
                     /locus_tag="VW854_000447"
     CDS             44019..47672
                     /locus_tag="VW854_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261737.1"
                     /translation="MATLDELKVMIDAEIAPFRKKMKEVENQVKGTSDQVKNATAKVR
                     EQSNSIGSAFGKLAKFAGFAILGKKLLDVGMYSTQTALEVSAAMNQIKRQMGESSQSF
                     LKWVNDNANAMNMGVGEATNYGAVYSNLFSGFIKDTNKLSAYTAKMLQTSAVVAEGSG
                     RTITDVMERIRSGLLGNTEAIEDLGINVNVAMIESTEAFKKFANGQSWQQLDYQTQQQ
                     IRLMAILEQATAKYGNTLSNSVNGRISLFKSLMKDAALNLGNSMLPIINAIMPVLNSF
                     AMVLKNVTAKLAEFIALMFNKKATVKDGVGGAVGDMGNAMKDAAGGAGDLADAVDDAG
                     DSAGGLADNLGDSAKNAKKAAKELLGLLGFDEINILQKPKDDDAGGSGGGGKGGKGKG
                     GGGGPFKDILPEVELTDMDNKFKSIFDGLGDKLKGLFDLFKKGFDAAFRPEGIKRIKT
                     ALDQIAKTMGEIATDPRVVNAFNRMAEKIAYALGQVTGSITTIGLGIGVFLAESIANG
                     LGRQKERIIRALVALFDNVGNLSEAVGNIAQDFSSAFYDVITSTGAVRIGSAIVSTLL
                     SLTSTIVEVGSKLAGSLFKGFEKVVVTSAPKISSVFQSLLDTVAPVFESIERSVNKFG
                     DGLSRVYDEHVVPAINSIANAFNGLIDIIQILWENSWQPFAEFLSGVFGVSIEGISDL
                     LGGGLLATLGLLADAIKLVADGFTVFSDWCKENKEPIVALITTWQTINFLSWAEQAGG
                     LAGAFSLLGSKISSIVGGIKNLGLAIKALTFDKLVSFAETIYLNTLYAKDFVVNSGKT
                     IAQLGKTALELGKSALAWTAHAAKMGLATAAEFAHSVAAGVATAATWAFNAALAVLTS
                     PITWIIAAIAALIAIGVLLYQNWDTVVEFAKTAWQGLCDFISGICQAIGEFFSGLWTK
                     LQEIFEPIGQWFGEKFQQAWDAIVNIFSGIGEWFSGVFQGAWDAIVNIFTPIGSWFGQ
                     RWADVTSALANIGAWFTDMFQKAWTGLTNIFSKLGSWFGERWNDVTSALSKVASWFGD
                     IFGKAFDAVKNAFSSIGDFFKGVWDTVKSIFVNAGQMVGEAVGGAFKSAVNAVLGTIE
                     NVVNGFIGMINGVLGVVRNLPGLGWVGSVSTVSLPRLARGGIVDSPTIAMIGEAGKEA
                     VVPLENTGFIQTLGRVVSSAVVNAMAGVSPQGGFSGDGDIVIQIAGHEFGRVAIQEIN
                     KEHERAGQTLLKI"
     gene            47681..48031
                     /locus_tag="VW854_000448"
     CDS             47681..48031
                     /locus_tag="VW854_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261738.1"
                     /translation="MAQLTINGVAVKPPKYFQVGIQDIDGETGRNANGDMMRDRITTK
                     RKLDCEWGMMTQGELSQLLHAVSSEFFEVSYPDPMDGQVTKTFYVGDRTAPSYTFTEK
                     FKPWSGAKFNLVER"
     gene            48036..>51656
                     /locus_tag="VW854_000449"
     CDS             48036..>51656
                     /locus_tag="VW854_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S74"
                     /protein_id="HEV0261739.1"
                     /translation="MDALTRRQFDRAMFAKERTLAIRVGDYASRDIKEASFEYGYIKG
                     DTYKPGGTCAGSGKITFTSIITTFNKLDTLHPEIGLLVGDTYQWVKMGEYFINDIEID
                     RNRNTTTLELMDGMFKLNREYVTDLHFPAEVREVIQEICLKTGIELADDYFGISAMRY
                     HIEQVPEGKKLSFRDMLSAMTQMIGMSCFFNREGKMEIRDLTESNITINADSYFLHGL
                     TKSEIEYQIAGITCKTDKKSLTVGMKTGRSLELDNVFMTQSALNDLYYKLKNLTYYPY
                     NLNYQGHLLLEVGQWVTIQTNKKETFKVPVLSQSFTFKGGLRGRISADSKAGNDTQYS
                     YEGTITKQIKQQDGIEAKIQAQIEATDKDFDQKVDKIKKDFNDQVELAKARAEEVKRE
                     LSDTINQRFNSFDNGPLKEAKRRAEEALRNAGASSLLAQEAKRIGLDSVARLEAFKSQ
                     TTSAQMALSGDLDVLKRTIANDIRPKQAQAEAEIAKQVEALSRTKNELSGASTLLAQE
                     AKRIELDSVARLEAFKSQTTSAQTALSGDLDVLKRTIANDIRPKQAQAEAEIAKQVEV
                     LSRTKNELSGVKSAQATYEETTTRRLSELTNLANGKASKSELTQTAEELASKIASVQA
                     SGRNLFLNSLFKQDIPKTGIWTTSTYTATIDSESKYLGHKALKIIGLNPSGRDGGNPK
                     VTYPALGQFGKVIPGSTTNQDVTISFYAKANKNGIMLRSRLGNIGYKTGNVTLSTEIK
                     RYVVHIPKGWTNESKRTTNEWLFNFNQEGTVWIWMPKFEISDVDTSYSEAPEDIEGQI
                     STVESNFKQRANSLEAGVSRLTEGLRTKADISSFNVAAENIRQSVKSLETDTQNKLNQ
                     KLSQAEFEVRAGSIRQEILNATKNKASKSELTQTAEELSSKIASVQVGGRNYIRGTKR
                     MMLARGLWASGTFRPSGAGTAKTIDVSDSSVTGFDKAIRLTSSNARDQIGIAQDGFYI
                     SQGTYTMSCWVKGRRGQKVKLQTYWQANDNSGISPIFTLKDENWTKLSFTSARNRAGV
                     ASIGYVYLVNAEVGEYLDVLAPQLEDGSLATSSKEAPEDIEGQISTVESTFKQRADSL
                     EAGVSRLTEGLRTKADISSFNVAAENIRQSVKSLETDTQNKLNQKLSQAEFEVRAGSI
                     RQEILNATKDKASKSELTQTAEELASKIASVQASGRNLFLNSLFKQDISKTGIWTTST
                     YTA"
BASE COUNT        16225 a         8980 c        11808 g        14643 t
ORIGIN      
        1 atttgaagga tataagaata aaatagtgga gataaaaggt atctatagtc cgttggaaaa
       61 attcttgttt tcaacccaga gtaaaataat aattgaaaag tgagataaaa tggaattttg
      121 gaatgacaca attcaaaccg tcaaagagtg gcgaccagtc aattattcag ttgctgaaga
      181 tgaagacttt gaagctgtga agaaaaaagc aattagaaat tttcaagatt atctaaaatt
      241 acttgatgag aatactgaag aaggctacca agaagttata aggttattta catttagcaa
      301 attctatggt gaagaactgt gtaacgatga agaactgaac aaattgagta gagatataag
      361 aggaagatta aaaaaattcc gttcctaaaa agttactaaa aatcaaaatt ttattttttc
      421 aaacaatata aatggttttt tataatgttg attatgctga tttataaaga tataaaatct
      481 taaacactaa actcaaaatc cagtgtccgc aaggacgtgc cggttcgacc ccggccgccg
      541 gtatagtata gtgttaggaa cgttgttatt cttcgttcct tttttatatt atttttggta
      601 taattatagt tattcaaatt ttatttagat tgagaaagtg tagggtagta tgtcttgttc
      661 tatcgattta ttaaaacatc ggtatttgaa aaatattaaa gaaaatcctg aattgtttgt
      721 cggaattgag ttggagtatc ctgttgcaag tttagaaggg gatgctacag atgttgaagt
      781 tataaaggat ctatttcatt atttagtttc tactttggat ctcaccgtag caaaggtaga
      841 tgattttggc aatctgatcc agttagtaga tccgataagt caggatgcta ttttatttga
      901 agtttcctat acaacgattg agtttgcatt tggtaaggct gaaacgattc aagaggtcga
      961 aaatcgtttc aataattata tgaatgtaat tcagagaaag ttagctgaat caaatcatgc
     1021 tattgttggc tgtggtatcc atcccaactg ggataaaaat gagaattgtc cagtggctta
     1081 tccacgctat cagatgttga tggattattt gaatttgagt agaaatatta ttaaatcaga
     1141 tttacatcat ttccctgaat atggtacttt tatctgtggg agccaggttc agctggatat
     1201 ttcaaaaacc aactacttgc gggtgattaa tgcttttact caaattgaag cggctaaggc
     1261 ttatttattt gcaaactctg agttttcggg tgcggattgg gatacgaaaa tttcaaggga
     1321 tattttctgg gaagaatcta tgcatggtat ctatccagag aatgttgggg tcaatgctag
     1381 actccttaat gatgaagctg atttttttga ctatctaaat cattctgcga tttttactgc
     1441 ggaacgtgat gggcagacct attattttta tcctattcag gctggggact atttggctac
     1501 gcctgaaatc caagcatttg ctctgaatgg ggatgaggtt attatttacc cccaagagaa
     1561 ggattttgaa actcatcgta gttaccagta ccaagactta acgactcgag gaacagttga
     1621 gtttcgtagt gtgtgtacac agccacttga taggactttt gcttctgcag cttttcactt
     1681 gggattattg gttaatttag acaagttaga agcttactta gaaacagcac ctttctttaa
     1741 agtatttggt tatgattaca agtttttaag gagacaattt tctaagaaaa atcttacaga
     1801 tgaggaagaa actatgatta ttgaattttc caaagactta ctcctactag ctgaggaggg
     1861 actagtggtg agaaataagg aagaaatgac ctatttacag cctttgagag aagaattgag
     1921 cctataattt ctcttataaa gggagaattt tctgaaaaat catgatataa tggacgagac
     1981 tatagataaa ggatagagag taatgacatt agtttatcaa tcaacgcgtg atgccaacaa
     2041 tacagtaact gccagccaag caattttgca aggtttggcg acggacggcg gtttgtttac
     2101 accggttact tatccaaagg tagatttgaa ctttaacaaa ttgaaagatg cttcttacca
     2161 ggaagttgct aagctagttt tgtcagcatt tttagatgac tttacagctg aggagttgga
     2221 ctactgtatc aacaatgcct acgatagcaa atttgatact ccagctattg caccattagt
     2281 gaaattagat gggcaataca atttggaact tttccatggt tcaacgattg cctttaagga
     2341 tatggccttg tctattttgc catactttat gacgactgct gctaagaaac atggtttgga
     2401 gaacaagatt gttatcttga cagcgacatc tggtgacacg gggaaagctg ctatggcggg
     2461 gtttgcggat gtgcctggta ctgagattat cgtcttttat ccaaaggatg gtgtcagcaa
     2521 gattcaagag ttacaaatga ccactcaaac tggcgacaat actcatgtta ttgctattga
     2581 tggtaacttt gacgatgcgc aaacaaatgt gaagcacatg tttaacgacg tggctcttcg
     2641 tgaaaaattg actaccaaca agttgcaatt ttcatcagct aactctatga acattggtcg
     2701 tctggtgcca caaattgttt attatgttta tgcttacgct caattggtca agactggtga
     2761 gatcgtggct ggtgataaga ttaacttcac agtaccaaca ggaaactttg gaaatatctt
     2821 ggctgccttt tatgccaaac aaatcggtct cccagttggt aaattgatct gtgcttcaaa
     2881 tgacaacaat gttttgacag acttcttcaa gacacgtgtc tatgacaaga aacgtgagtt
     2941 taaggtaaca accagcccat ctatggatat cttggtatct tcaaacttgg agcgtttgat
     3001 tttccatctt ttggggaatg atgcggttaa gacggctgaa ctcatgaatg ctttgaatag
     3061 ccaaggtcaa tatgagttga ctaattttga tgcagagatt ttggacctct ttgcagctga
     3121 atatgcgact gaggaagaaa ctgcggcaga aatcaagcgt gtttatgagt cagattctta
     3181 tatcgaggat cctcatacag cgattgcctc agcagtgtat agaaaatacc aggcgactac
     3241 tggagatgcg actaagacag tgattgcttc aacagctagt ccctacaagt tcccagtagt
     3301 tgcagtagaa gcgctaacag ggaaagttgg cttgactgat tttgaagctt tggctcaatt
     3361 acatgaaatc tcaggcgttg cagtgccacc agcagttgat gggcttgaga cagctcctgt
     3421 tcgtcacaag acaatagtgg cagctgctga catgcaagta gcggttgagg cttatttagg
     3481 actttaagat agagggagta aactcggttg ggaaaccaac tgagtttctt ttcatcagga
     3541 ggagagattg tttaagaaaa ataaagacat tcttaatatt gcattgccag ctatgggtga
     3601 aaactttttg cagatgctaa tgggaatggt ggacagttat ttggttgctc atttaggatt
     3661 gatagctatt tcaggggttt cagtagctgg taatattatc accatttatc aggcgatttt
     3721 catcgctctg ggagctgcta tttccagtgt tatttcaaaa agcatagggc agaaagacca
     3781 gtcgaagttg gcctatcatg tgactgaggc gttgaagatt accttactat taagtttcct
     3841 tttaggattt ttgtccatct tcgctgggaa agagatgata ggacttttgg ggacggagag
     3901 ggatgtagct gagagtggtg gactgtatct atctttggta ggcggatcga ttgttctctt
     3961 aggtttaatg actagtctgg gagccttgat tcgtgcaacg cataatccac gtctgcctct
     4021 ctatgttagt tttttatcca atgccttgaa tattcttttt tcaagtctag ctatttttgt
     4081 tctggatatg gggatagctg gtgttgcttg ggggacaatt ctgtctcgtt tggttggtct
     4141 tgtgattttg tggtcacaat taaaactgcc ttatgggaag ccaacttttg gtttagataa
     4201 ggaactgttg accttggctt taccagcagc tggagagcga cttatgatga gggctggaga
     4261 tgtagtgatc attgccttgg tcgtttcttt tgggacggag gcagttgctg ggaatgcaat
     4321 cggagaagtc ttgacccagt ttaactatat gcctgccttt ggcgtcgcta cggcaacggt
     4381 catgctgttg gcccgagcag ttggagagga tgattggaaa agagttgcta gtttgagtaa
     4441 acaaaccttt tggctttctc tgttcctcat gttgcccctg tcctttagta tatatgtctt
     4501 gggtgtacca ttaactcatc tctatacgac tgattctcta gcggtggagg ctagtgttct
     4561 agtgacactg ttttcactac ttgggacccc tatgacgaca ggaacagtca tctatacggc
     4621 agtctggcag ggattaggaa atgcacgcct ccctttttat gcgacaagta taggcatgtg
     4681 gtgtatccgc attgggacag gatatctgat ggggattgtg cttggttggg gcttgcctgg
     4741 tatttgggca gggtctctct tggataatgg ttttcgctgg ttatttctac gctatcgtta
     4801 ccagcgctat atgaacttga aaggatagga aatgcaaaaa acagctttta tttgggattt
     4861 agacgggact ttattggact cttacgaagc gattttatca gggattgagg agacttttgc
     4921 tctgttttct attccttatg ataaggagaa ggtgagagag tttatcttca agtattcggt
     4981 gcaagatttg cttgtgcggg tggcagaaga tagaaatctg gatgttgagg tgctaaatca
     5041 ggtgcgtgcc cagagtctgg ctgagaaaaa tgctcaggta gttttgatgc caggtgcgcg
     5101 tgaggtgcta gcttgggcag acgaatcagg aattcagcag tttatatata ctcataaggg
     5161 gaacaacgct tttaccattc tcaaggactt gggggtggaa tcctatttta cagagatttt
     5221 aaccagtcag agtggctttg tgcggaagcc aagtccagaa gcggctacct atctgctaga
     5281 taagtatcag ttgaattctg ataatactta ttatataggg gatcggactc tggatgtgga
     5341 atttgcccag aatagtggga ttcaaagcat caacttttta gagtctactt atgaagggaa
     5401 tcacaggatt caagcgttag cagatatttc ccgtattttt gagactaagt gataaaaaga
     5461 ttgtgtcagt tttgtgacag agacctaaca aactatttca agtaacctag tttgttacaa
     5521 ggaatagaca gttctgttaa ataggcccga gagggctttt tttctacatt ttttgtgtta
     5581 tgatagacag gtactcattt gaaaggaatt tgaaagaatg aagaaaagaa tgttattagc
     5641 gtcaacagta gccttgtcat ttgccccagt attggcaact caagcagaag aagttctttg
     5701 gactgcacgt agtgttgagc aaatccaaaa cgatttgact aaaacggaca acaaaacaag
     5761 ttataccgta cagtatggtg atactttgag caccattgca gaagccttgg gtgtagatgt
     5821 cacagtgctt gcgaatctga acaaaatcac taatatggac ttgattttcc cagaaactgt
     5881 tttgacaacg actgtcaatg aagcagaaga agtaacagaa gttgaaatcc aaacacctca
     5941 agcagactct agtgaagaag tgacaactgc gacagcagat ttgaccacta atcaagtgac
     6001 cgttgatgat caaactgttc aggttgcaga cctttctcaa ccaattgcag aagctccaaa
     6061 agaagtagca tcaagttcag aagttacaaa gacagtgatt gcttctgaag aagtggcacc
     6121 atctacgggc acttctgtcc cagaggagca aacggccgaa acaagcagtg cagttgcaga
     6181 agaagctcct caggaaacga ctccaggtga gaagcaggaa acacaagcaa gccctcaagc
     6241 agcatcagca gtggaagcaa ctacaacaag ttcagaagca aaagaagtag catcatcaaa
     6301 tggagctaca gcagcagttt ctacttatca accagaagag acgaaaataa tttcaacaac
     6361 ttacgaggct ccagctgcgc ccgattatgc tggacttgca gtagcaaaat ctgaaaatgc
     6421 aggtcttcaa ccacaaacag ctgcctttaa agaagaaatt gctaacttgt ttggcattac
     6481 atcctttagt ggttatcgtc caggagacag tggagatcac ggaaaaggtt tggctatcga
     6541 ttttatggta ccagaaagtt cagaattagg ggataagatt gcggaatatg ctattcaaaa
     6601 catggccagc cgtggcatta gttacatcat ctggaaacaa cgtttctatg ctccattcga
     6661 tagcaaatat gggccagcta acacttggaa tccaatgcca gaccgtggta gtgtgacaga
     6721 aaatcactat gaccacgttc acgtttcaat gaatggataa acccgacttg gtaacatcat
     6781 tttgacgaat gagatctagc tttcgtgata ggaagcgatt ctcgttcgtt ttttctttgt
     6841 catactcttc gaaaatctct tcaaaccacg tcagttttat ctgaaacctc aaagctgtgc
     6901 tttgagcaac ctgcgactag ctttctagtt tactctttga ttttcattga gtatcaattt
     6961 gaatggaaaa tggaaagtta ttatcttgta ataatccaag caacattctt gcaatctatt
     7021 ttactttata tcacaattga ttggtcaact attgataagt caatggatag gaggaagaaa
     7081 tgatagagat tcaagattta ctgtatcaac tccgcttgtc tgagcaagcg agtacgcaat
     7141 tgtttgaaaa aggcttggga ttagtttgac acggtatcag attttactgt ttttgctgga
     7201 gcattctcct tgtaaccaaa tggcggttca ggagcgtttg aaaattgatc aggctgcttt
     7261 gacacggcat ttcaagattt tggaaacgga aggtttggtg gagcgtcatc gtaatcctga
     7321 aaatcagcgg gaagtgttgg tagaggctgc gaagtatgcc aaggagcagt tagtggtgaa
     7381 tccccctctg caacatatca aggttaagga agagatggaa agtatcttaa cagagtttga
     7441 gagaacagaa ctcagccgtt tattaaataa attggttttg ggtattgaaa atatagaaat
     7501 ttaaggagaa atagatgtca attattttaa caacgatcgt tgctttggag catttttaca
     7561 ttttttattt ggaaagtatt gccacgcaat cagatgcgac tagtcgtgta tttaatatgg
     7621 aaaaggaaga attggctcat ccgtcagtaa gttcattgtt caaaaatcaa ggaatttata
     7681 aggctctgct aggagtcttt ctcttgtatg tcatttattt ctcacagaat ttagaaattg
     7741 tgactatttt tgtcttattt gtgattggtg ctgcgactta cggctcttta acagcggata
     7801 aaaaattatt ttgaagcaag gtggatcagc tattttggcc ttgattagta ttttactctt
     7861 taaatacact tgaaggttga ttctaatctc gctaatcctt tttaatccag aataagggaa
     7921 atatgttata cttgttttta agaaaaaagt ctcattgaat tggttttgag gagttagaaa
     7981 tgaaagtatt agtgacaggt tttgagccct tttgaggcca ttaaaggttt accagctgaa
     8041 atccatggtg ctgaggtccg ttagctagag gtgccgacag tttttcacaa atctgctcaa
     8101 gtattggaag aagagatgaa tcgttatcaa cctgactttg tcctttgtat tgggcaagct
     8161 ggtggaagaa ctagtttgac acctgaacga gtggccatta atcaagacga tgcacgcatt
     8221 tctgataacg aagataatca accgattgac cgtcccattc gcccagatgg tgcttcggcc
     8281 tactttagta gtttgccgat taaagcgatg gttcaagcta taaaaaagag ggcttaccgg
     8341 cctctgtttc caatacggca gggacttttg tctgcagcca tttgatgtat caggctctct
     8401 atttggtaga aaagaaattt ccatatgtta aggcaggttt tatgcatatt ccttatatga
     8461 tggaacaggt ggtgaacaga cagactattc cagctatgag tttagtggat attcggcgag
     8521 ggatagaagc agcaatcggc gctatgatag aacatggaga tcaggaactc aagttggtag
     8581 gcggagaaac tcattgatag aaaaaagctt gaggggaaaa accttcaagc ttttggacgt
     8641 tttcgagcca atactgctcg gtaaaacata attttagtgc attggatata aggtaggagt
     8701 gaaaaactag caatgccaaa ggtaatccaa ttgaggaagt accaaggaag aagctgtaaa
     8761 tctaggacaa agcgctggaa cttgtagccc ttcataaagg aacggctagt ttttaggatt
     8821 cgtcttggtg ggacctgtcc taggtctaga ctataacaga gaagaaattc cacctgtgaa
     8881 taggcataat actgtggaat atagaggata tttcctacaa tgatcaagat gagacttgca
     8941 agaaagtaga gtccaaagac catgaggaaa cgctcggttt caactgatga gagatctaga
     9001 tttggaaact caggatgtag ggtgacgaat tttttggcta aaaagctact ataaaagagg
     9061 aggtaaatcc caagtaaatt agggatactc cataaaaaga gatagaaacg tttgagaagt
     9121 agggtcaaaa aggtttgaga aaagcgctcc tcatcaaaga gagctaggct gttttttaca
     9181 gatggctccg ttttagaatc tttcatgagt gtcagtgttg catagacgga actggtcaaa
     9241 agaatagtcc cgataaagga gactagtaga ggaaagaggt aggcttgaag tatttggcca
     9301 agtatgctga aaaatggctg ttctaaaaca gtcccgtgga tccgagataa gggattaaga
     9361 aaactagata agatgaccag catactggga aggatataga ggagaaagag acggggggtg
     9421 tcagcctgaa aatgttttga ctcctgacga attgttttta aatcaatttt tggatagttc
     9481 attctcttat tataccatag ttcttataca tagttcgtga cagttcctac tttttttgat
     9541 aaaatcatac agtgtgtcct tgggcacact gtatgaactg ggactgtctt tcccagcttc
     9601 ggaggtaaaa aatgtcagat tcaccaatca aatatcgttt gattaagaaa gaaaaacaca
     9661 caggagctcg tctgggagaa atcatcactc cccacggtac ctttccgaca cctatgttta
     9721 tgccagttgg gacacaagcc actgtcaaaa ctcagtcacc tgaagaattg aaggagatgg
     9781 gttcgggaat tatcctatca aacacctatc atctctggct tcgccctgga gatgaactca
     9841 ttgcacgcgc tggtggtctc cacaagttca tgaattggga ccagcctatc ttgacagata
     9901 gtggtggttt tcaggtttat tctttagcag atagccgtaa tatcacagaa gaaggagtaa
     9961 cctttaaaaa tcatctaaat ggttctaaga tgttcctatc cccagaaaaa gccatctcta
    10021 ttcagaataa tctgggttca gacatcatga tgtcctttga tgaatgtcct cagttttatc
    10081 aaccttatga ctacgttaag aaatcgatcg agcgtaccag ccgttgggct gagcgtggtt
    10141 tgaaggctca ccgtcgtcca catgaccaag gtttgtttgg aattgtgcaa ggtgcaggat
    10201 ttgaagacct tcgccgccaa tcagctcatg atcttgtcag catggatttc tcaggctact
    10261 ctatcggtgg tttggcagtg ggagaaaccc atgaagagat gaatgcggtc ttggacttta
    10321 caactcaact gctgcctgaa aataaacctc gttatctgat gggtgtggga gcgccagata
    10381 gcttgatcga tggggtcatt cgtggggtgg atatgtttga ctgtgtctta ccgactcgaa
    10441 ttgctcgtaa cgggacttgt atgaccagtc aaggacgttt ggttgtgaaa aatgcccagt
    10501 ttgctgagga ctttacgcca ctggatcctg agtgtgattg ctacacatgt aataactata
    10561 cacgcgctta ccttcgtcac ctgctcaagg ctgatgaaac ctttggtatc cgcttgacta
    10621 gctaccacaa tctttacttc ttgcttaacc tgatgaagca agtgcgacaa gccatcatgg
    10681 atgacaatct cttggaattc cgtgagtatt ttgtggaaaa atatggctat aataagtcag
    10741 gacgtaattt ctaaaatgga attgatataa aaaaatccta agttttctct taggattttt
    10801 cttctttttt tgatagaata aagtgtacaa tgaaaggaag aataaactcg tatgcgcatt
    10861 aaatggtttt ccttgattag gattataggt ttacttttgg tactcttgta tcacttcttt
    10921 cagacgatct ttcctggagg atttttcggg gtagatgtct ttttcacatt ttcaggtttc
    10981 ctgattacag ctctactcat tgaagaattt tctaagaaca atgagattga tttgatagga
    11041 ttttttagaa gacgctttta tcggattgtg ccacctgtgg ttttgatggt cttggtgacc
    11101 atgcctttta ctttcttggt tcgccaagac tatgttgctg gaattggtgg ccagattgcg
    11161 ggcgtcttag gctttatgac caacttctat gaactcctaa caggtgggag ttatgaatct
    11221 cagttcattc ctcatttgtt tgttcataat tggagtttgg cagttgaggt tcactactat
    11281 attctttggg gattggcagt ttggttctta tccaaacaag ctaaatcaaa tggtcagttg
    11341 aaggggatgg tctttctctt atctgctgtt gccttcttga tcagtttctt ctccatgttt
    11401 attggtagtt ttctagtgac ctcttattcc tctgtttatt tctccagttt aactcatgtc
    11461 tatccattct ttttaggaag tatgttagca actattgtag gcgttcgtca gacgacttcc
    11521 ctcgtcaagc agttggataa aatctgggat ttacgaaaga ctttggtagt ttttggagga
    11581 ggctttggtt tcctagttct tttgactttc tttgtcaaat tcacttatct ttttgcctat
    11641 cttatcggct tcttacttgc cagtcttgca gctcttgcca tgattctggc ggcgcgtgtc
    11701 ttacatgaaa agacacatca tatacaggag tcgaagatta tcagcttttt agcggatact
    11761 agctatgcgg tttatctttt ccattggcct ttctatatca ttttctcaca gttgacatca
    11821 aatcttcttg ctgtattact gactctgatt tgttcttatg gctttgccag tctgtcattt
    11881 tatgtattgg aaccttggat tgcaggcaag aacacaccta ttgtccaaac ccttcgtccc
    11941 ctgccttata ttcacgcaat tcttgcagca ggtacaggaa tcttgaccat cattgtctgc
    12001 acggtgacct tgttggcacc acaagtggga gcgtttgaga cagacttgac tgtcaatggc
    12061 ttgaagcaag ctgcaacaaa tattggccag accaaggtga tggcagaacg ggcagatgca
    12121 aacagtttgg gaattgctga tggcactatg ttaattggtg actcagtggc tttaagggca
    12181 aatacagcac tacagacagc tcttcctgga gcacagatta acgcgcaggt cagcgtaaca
    12241 accaagaccg caaatgaaat catgctaaat aatagccaga ataaattttt acctaagacg
    12301 gtggtcattg cgactggggt aaataatcct gagaattaca aggatgactg ggacagtatc
    12361 gtgaaaaatc ttcctaaggg acaccatatg attttggtga ctccttatga gggagataag
    12421 acaaaagaga cctatgccat cgttgagaag gctgctgcct atatgagaga attggcagag
    12481 aagacacctt acattacgat agcagattgg aatcaagttg cgaaagagca tccagaaatt
    12541 tgggctggaa cagaccaggt tcatttcggg agtgagagta gcactatcga agcaggagca
    12601 aaattgtatg cagatacgat tgccacagct ttgcagacag ctcaagacaa gccggttaaa
    12661 tcaaaataac ttgtattaaa aaagagaaga gttggagaaa tccagctctt ttaaaatatc
    12721 tccaaaaaat aggtctatac catttacaaa taaaaaagaa aggtttataa tgtaattgac
    12781 ataataaatt gtagaatcaa tcttttaagg aggttaacat tatgcctaac tatattaaag
    12841 cggatcagtt tttctaccca cacggagttc gtcgaggtgg ttacttggaa cttgtggacg
    12901 gcaagtttgg gaaacatgta gagcagattc ctgaaggggc tgaggtgatt gactatacag
    12961 gttatagcat tgccccaggt cttgtggata ctcatattca tggatatgca ggtgtagatg
    13021 tgatggacaa caacattgaa ggtacattgc atactatgag tgaaggactt cttagtaccg
    13081 gtgttaccag tttcttaccc acaactttaa cagccactta tgagcaattg cttgcagtca
    13141 ctgaaaatct tggaaaccat tataaagaag caacaggtgc taagattcgt gggatttatt
    13201 atgaaggtcc atatttcaca gaaactttta agggggcaca aaatccaact tatatgagag
    13261 acccgggtgt tgaggagttt cattcttggc aaaaagcggc aaatggcttg cttaataaaa
    13321 ttgcccttgc accagaacgt gatggggtgg aagactttgt tcgtacagtt acgggcgaag
    13381 gtgtgacggt tgctcttgga cattcaaacg cgacttttga tgaagccaaa aaagcagtcg
    13441 atgctggagc gagtgtttgg gtgcatgcct acaatggaat gcgtgggttg actcaccgtg
    13501 aattgggtat ggttggagcc atgtaccaat tgccacatac ctatgcagag ttgatctgtg
    13561 atggtcacca cgtagatcca aaggcttgcg aaattcttat caaacaaaaa ggaacagaaa
    13621 acatcgctct tatcacagac tgtatgacag ctgggggatt ggaagacgga gattatatgt
    13681 tgggagaatt cccagttgtc gttgcaaatg gaactgcacg cctcaaatcg acaggtaact
    13741 tggcaggttc tatcctcaaa ctcaaagatg gtttgaagaa tgtggtcgaa tggggaattg
    13801 cgaatccgca tgaagcagtc atgatggcca gcttcaaccc agctaaatcc gttcacatcg
    13861 atgacgtctg tggccaaatc cgtgaaggct acgacgctga cttcatcgta ttagataaag
    13921 atttggaatt ggtagcaacc tacctagatg gcgtaaaacg ttatcaagca taagagagaa
    13981 agcagaagtt agagactaac ttctgctttt ttatgcaggg tactttactg gtaaataaaa
    14041 agttaaaaaa atcacaaaaa aagcttgaag aaacaaagaa aaaggcttac aattgaacta
    14101 taaatattac aaataaaaaa acggaggagt gctttatgaa agcctatact tatgttaaac
    14161 caggacttgc ttcttttgtt gatgtagaca aaccagttat tcgcaagcca acagacgcta
    14221 ttgtgcgtat tgtaaaaacc actatttgtg gaacagacct ccatattatc aaaggggatg
    14281 ttcctacttg ccaaagtggt accattcttg gccacgaagg gattgggatt gttgaagaag
    14341 ttggggaagg agtttccaac ttcaaaaaag gtgacaaggt cttgatttct tgcgtctgtg
    14401 cctgtggtaa atgctactac tgtaaaaaag gaatttatgc tcactgtgaa gacgaagggg
    14461 gttggatttt cggtcacttg attgatggta tgcaggctga atatctacgt gtccctcatg
    14521 cagataatac tctttaccat actccagaag acttgtcaga tgaagctttg gttatgctgt
    14581 cagacattct gcctactgga tatgaaattg gtgtcttaaa agggaaagta gaacctggtt
    14641 gcagcgtagc cattattggt tcaggtccag ttggattggc tgctctttta acagcccaat
    14701 tctattcacc agctaaattg attatggtag acctagacga taaccgcttg gaaactgccc
    14761 tatcattcgg tgcgactcat aaggttaatt cttcagaccc tgaaaaagcc attaaagaaa
    14821 tttatgattt gacagatggt cgtggtgtgg atgtcgctat cgaagctgtt ggtattcctg
    14881 caacatttga tttctgtcaa aagattatcg gtgtagacgg aacggttgcc aactgtggtg
    14941 tgcatggtaa accagttgaa ttcgatttag ataaactttg gattcgcaac atcaatgtaa
    15001 caactggttt ggtatctaca aatacgactc cacaattgtt gaaagcactt gaaagtcata
    15061 agattgaacc ggaaaaattg gtaactcact atttcaaact cagtgaaatt gaaaaagcct
    15121 acgaagtctt cagtaaggca gcagaccacc atgccattaa ggtcattatc gaaaacgata
    15181 tctcagaagc ctaagtagta aaaatatttt tgtacataag taaatagaaa ttcagtcatc
    15241 catcagatgg ctggattttt tatcaaaaaa ttaagaaatg agcatatttc tttccttgtc
    15301 tggcggaatt ggttataata tacggtacaa aggaatgaat gaatatgtat cgtgttatag
    15361 aaatgtacgg agattttgaa ccgtggtggt tcttagaagg ttgggaagaa gatattgtag
    15421 caagtagaaa atttgaccag tattatgatg ctctcaaata ctacaaaact tgctggttta
    15481 gattggaaca agaatcgcct ctttataaaa gtagaagcga cttgatgacc attttttggg
    15541 acccggaaga ccaacgctgg tgtgatgaat gtgatgagta tttacaacaa taccattctt
    15601 tggctctttt gcaggatgag caggttatcc cagacgaaaa actacgctca ggctatgaaa
    15661 aacaaaccag tcaggaaagg aatcgttctt gccgtatgaa attaaaatag agaaaagtaa
    15721 cttttttgga gttgcttttt ttatttttct aactctttgc gaatagtata ggtgaggagg
    15781 taagtatggt tcaagaaatt gcacaagaaa tcattcgttc agctcggaaa aaagggacgc
    15841 aggatatcta ttttgtccct aagttagacg cctatgagct tcatatgagg gtaggagacg
    15901 agcgctgtaa aattggtagc tatgattttg aaaagtttgc agccgttatc agtcacttta
    15961 agtttgtggc gggtatgaat gtgggagaaa aaagacgtag tcaactgggt tcctgtgatt
    16021 atgcctatga ccataagata gcgtctctac gtttatctac tgtaggcgat tatcgggggc
    16081 atgagagttt ggttatccgt ttgttgcacg atgaggagca ggacctgcat ttttggtttc
    16141 aggatattga agaattaggc aagcagtaca ggcaacgggg actctatctt tttgctggtc
    16201 cggttgggag tggtaagacg accttgatgc atgaattgtc caagtcactc tttaaaggac
    16261 agcaagttat gtccatcgaa gatcctgtcg aaatcaagca ggacgacatg cttcagttgc
    16321 agttgaacga agcaatcggc ctaacctatg aaaatctaat caaactttcc ttgcgtcatc
    16381 gaccagatct cttgattatc ggagaaattc gtgacagcga gacggcgcgt gcagtggtca
    16441 gagctagttt gacaggtgcg acagtctttt caaccattca cgccaagagt atccgaggtg
    16501 tttatgagcg tctgctggag ttgggtgtga gtgaagaaga attggcagtt gttctgcaag
    16561 gagtctgcta ccagagatta atcgggggag gaggaatcgt tgactttgca agcagagatt
    16621 atcaagaaca ccaagcagcc aagtggaatg agcaaattga ccagcttctt aaagatggac
    16681 atatcacaag tcttcaggct gagacggaaa aaattagcta cagctaagca aaaaaatatc
    16741 atcaccctat ttaacaatct cttttctagc ggttttcatc tggtggagac tatctccttt
    16801 ttagatagga gtgctttgtt ggacaagcag tgtgtgaccc agatgcgtgt gggcttgtct
    16861 caggggaaat cattctcaga aatgatggaa agtttgggat gttcaagtgc tattgtcact
    16921 cagttatccc tagctgaagt tcatggcaat ctccacctga gtttgggaaa gatagaagaa
    16981 tatctggaca atctggctaa ggtcaagaaa aaattgattg aagtagcgac ctatcccttg
    17041 attttgctgg gttttcttct cttaattatg ctggggctac ggaattacct gctcccacaa
    17101 ctggatagta gcaatattgc cacccaaatt atcggtaatc tgccccaaat ttttctaggc
    17161 atggtagggc ttgtttccgt gcttgccctt ttagcactca ctttttataa aagaagttct
    17221 aagatgagtg tcttttctat cttagcacgc cttcccttta ttggaatctt tgtgcagacc
    17281 tacttgacag cctattatgc acgtgaatgg gggaatatga tttcacaggg aatggagttg
    17341 acgcagattt ttcaaatgat gcaggaacaa ggttcccagc tctttaaaga agtcggtcaa
    17401 gatctggctc aaaccctgaa aaatggccgt gaattttctc agacgatagg aacctatcct
    17461 ttctttagga aggaattgag tctcatcata gagtatgggg aagttaagtc caagctgggt
    17521 agtgagttgg aaatctatgc tgaaaaaact tgggaagcct tttttacccg agtcaaccgc
    17581 accatgaatt tggtgcagcc actggttttt atctttgtgg cactgattat cgttttactt
    17641 tatgcggcaa tgctcatgcc catgtatcaa aatatggagg taaattttta aaatgaaaaa
    17701 aatgatgaca ttcttgaaaa aagctaaggt taaagctttt acattggtgg aaatgtaagt
    17761 tactaaaatt atttatattt tccacttgat gacaatgtcc tcagctgtta cctggacctt
    17821 gtttataagc cctctaacaa gtaccttctg tttctcataa tccattgaaa acactttttc
    17881 agcgtttagc agtttcctca tatcagcctt tcttttgttc ttcctgagcg ctgaatcgtt
    17941 ttccaattca gtctcaagag tagccctcat gcttataaat tcggctgact tgctctgtaa
    18001 ttcttcaagg gtaatgcggt catctatgta tagatcgtta agtctgctca gtttctttga
    18061 tagctcctct atttgtttct tatagctctc acggtctatg gtctcagcat tgtctcctga
    18121 aaatattttg tccaggtaat cagcgtcatc ttgtagtttg cttatttctt ttagcacaga
    18181 ggcctctagc ttgtctttgt agtaaaatcc tgagtcacac tttttattgt cgttgtaggt
    18241 agtaacgcct ctcagcgttc gtgggtgcct ttgatggcat tcatattttt ttaacctgct
    18301 cccatctttc ctctttacgc ctaacataat ttttaaagga gcgccacaat atccacattg
    18361 ggcgataccg gatagaatgt acttagcttg gaatggtcta ggattgacat tctctgctgc
    18421 cgtcctttgt ctgattttta gctcagattg agtcttatcg tattcctctt ttgaaataat
    18481 cggctcatga ttacctggat aaatttctcc cttatactga ttgaaaccac aatagacagg
    18541 gttatcgagt atggttctga ccgcccgata actccaaggc acatgctttg ggtatttctc
    18601 atttagatca tccctcagct tagtaataga tctccctctc agataactct caaagataaa
    18661 tttaacagcc agagcctgag ctggattgat agtcacagtt ccagtttcct tgtgataatt
    18721 gtatccgtaa gatgttttag cccacatcat ggatttccca gcctttgccc gtcctatttt
    18781 cccaagttgc atacgttcct tgatttgttc cctttctagc tgagcaaaga cgctcaagag
    18841 tccaatcata gccttaccaa aaggagtaga ggtgtcaaag ttctcctgca agctcagaaa
    18901 ttcaatccca ttcttgatga atacatcctc aatcaagtga agcgtgtctt tttgactacg
    18961 gctaagacgg tccagcttat agactagaac tgtgtcaaat tttctttttt tagcgtcttt
    19021 gataagactt tctagcgctg gtctgtcagt attggatcct gagaaacctc catcagtata
    19081 tattttgtat acattccagt ctttaatatc gcagtagcta gagagcttgg ctttttgctc
    19141 atcgatagag tacccctcct caacctgtga ggtagtagat actctgacat atatagccac
    19201 tttattcatt gttttcattg aatttgtacc ccttttttga taaaataggt acaagaaaaa
    19261 cagcttttta atgctttttt cttgctccta gcctcacgct ctcggtcgcc aaacttctga
    19321 gcgtggggct tttttgagtt gtttccaaaa tggaaacagt tgctagataa aaagaaaagt
    19381 agccgtatca aatacggcta ccatcacgtt atggatctaa aatccaaatg taaactttat
    19441 ggagctaaac tcctaatagc tgtattgtaa tataattatt aagaaatgtc aagagattag
    19501 aaaaggtata attcttttaa cttttcgtta attttgtcca atgtgttatc agatactttc
    19561 atttttccga ttggatctaa tctatttttc tttaaaattc tatctttgct gattgtttga
    19621 aggttattac acttggcata agaacgctta atgtattttt tgtagtattg agttaattca
    19681 ataatgtcag ttatttctga ttgtgtccgt ttaaggttgt catcgtctac tatcttaggt
    19741 tgaattattt cgtttgaaaa ttgctctgta taagcttggt agacatccga taaggcagct
    19801 tcagtaattg cattattgga atctaaatat tttaggtagg caaataattc tttgtgtaat
    19861 ttttcaatat attcatcaag caaaatagaa ggatattccg caatgatttc gtctatgagt
    19921 acggtatcta tttgattttt agatgttaga ggaattactg tgagtgtttt tttataggga
    19981 ctatctacct tgtctaagac aatagcccaa tggttatttg ataattctcc gcctatattt
    20041 acaccgaact ctacgaatat cagagagcca cgattgaatt tccaatattt tcttttttga
    20101 gtcttagctt caaacaagaa ttgttcagat tgtcttttaa ctgctggtgc aagaaatcgg
    20161 tatttagaag atgtatgttt tgctttacca agtttataga gtttttcaac ttctatgtag
    20221 ttttgtttgg tttgttcaaa atatggattt tctttactca ttttatttct ctctatacac
    20281 actgacaact tccccaatag ttcggatgtc gtcattctct gtcaggtgga tttcttcata
    20341 gctattgttg aggctttgca agtaccaacc gccgttataa tcacgtttca gctttttaac
    20401 aaagttttta ccattgattt ggaaaatgcc gatgtcgttg atatctacct gattcgtgac
    20461 tttgataaag agcaggtcgt tatcttcaat cattggttcc atggagtcac cagccacctt
    20521 agcgatagtg ttgtagtctt cagggacatc ttcggcacgc agtcttactt ccatgtggag
    20581 gttgtcttcc tgaaacgttc catgtcctgc tgcaaccaag ccttctacgt aatcagtaat
    20641 gtagtcatcg ccatcttgag acttgtcaaa gatagagcta gctttggatt ttttttgttc
    20701 ttccagttgt tttttagcaa agtcaaggac tttctcctgc cttggttctt ctagtttgtt
    20761 gtagatcggc aagatttcag gctgttcatt tgatattgag ttacgctcac tatctggtat
    20821 acgttttttc tcaacatcat agcccataag ccaaggttcg cttacttcaa gtgttttaga
    20881 aagcaggtaa attctgtgtt gatcaggaga ctgtacacca ttaacatact gagaaagggt
    20941 acttttcccc atttttattc ctaattcttt ttgataaggt aatgattttt caagtatttc
    21001 aacttgtttt aaatttcttt cagacatgag ctgttttagt cgctcagatg ggttgctgct
    21061 cctcatgaaa cctcctcctt tttcctgttt ataagcctat tataaaccat cttgaacaaa
    21121 aattcaagag aaagttcata aaaaatgaat tttttgcttg actaggttca ttcaacgtga
    21181 taaaatatgt tttgtaaaac agttcatgaa aaatgaacaa gaaaggagta agctaatgag
    21241 taacgattat tcaaaacttg caggaaagat tgttgaaaag tatggcactc aatacaactt
    21301 tgctcaagca atggggctct cagagcgctc aatctcttta aagatgaata atagagttcc
    21361 ttggaaagat tttgagatgg caaaagcctc agagttgtta gatattgatg taaatcaatt
    21421 acatgaatat ttttttacac ctaaagttca tgttcgtgaa caaatagcat agaaaggagc
    21481 tcacatgaat gaactcatca acgtaaccct gaatgagaat caggagccag tagtatcagc
    21541 taggcagtta catcagacac tagaggtcaa gaaaaggttt agcgcttggt ttaaagcaaa
    21601 ctctaaatta tttatagaga atgaagattt tacaagtgtc caaacgggta cggttgtaaa
    21661 taacggagct atcaaacctc tacaagatta tgctctaact ttggatatgg ctaagcagtt
    21721 ggcgatgatg tctaggacga ttaaaggagc agagatccga caatacttta tacaagtaga
    21781 aaaagacttc aacagtcctg agaagattat ggcaagagca ttgctcatgg ctgatcagaa
    21841 agtccacaag ctagaggctc agattgaggc tgaccgtcct aaagtgctat ttgccgacgc
    21901 agtcagtgct agtaagtcat cttgtctgat tggtgaacta gctaaaattt tgaaacagaa
    21961 tgggattgac attggtcaaa acaaactttt tcaatggcta cgctctaacg gctacctaat
    22021 tagtcgtcgt ggagagtctt ggaaccaacc aacacaaaaa agcatgcagt taggtctgtt
    22081 tgaattaaaa aagacaaaca tcaatcatgc tgacggtcat acaactgtca gtacaactac
    22141 taaggtcact ggtaagggcc aacagtactt tatcaacaag tttctcaatc aggaatactt
    22201 gacaggataa aaacaaaaag cccctctgga acggcaattc cattgaggga ctaagcaaaa
    22261 tactttacga ggtaattata tcatgaaaac agtaaaaaag gaatgggagc cacggattgt
    22321 aaacatcatg gcagatggct ctcaagttga cgatctgaca ggatatgtca tccctgctgg
    22381 tcattcgtac tatgacatca ttttaggaat gcacaagcga gagttacaga aaggggctta
    22441 aatatgaggt atgcagtata taatcaggaa taccaacgag aactacactc agatgaacaa
    22501 ccactcagct caaaactcag aattgagctt gcaagctaaa gggttgctat tggtactgat
    22561 gtctaataag gatacatggc gcccttacat tgatgagctt tcaaaacgct ctaggaatgg
    22621 gcgtgacgct cacagggcag cttttgacga gctgaaagag gctggctata tccgtatcta
    22681 tcgcaagagc tttggtcgtg gcaaaggtat ccagaatttt cctttagttc aagatgtacc
    22741 aatttcagat agttattggg agtattgggt aagcaatctt gaaaaagagt tatccacaga
    22801 atagtagaag ggttcattta caacttactg actttacaaa gttgaaaagt tcaaaagttg
    22861 aattttacaa agttgaaaag ttcaaaagtt gaaaaatccg acactaataa taactaataa
    22921 ataacaagta ctaactatac aataatctaa gcctaacgga actaactaat aacaactact
    22981 aataaataac aatctagtag ttataagaat aatagaaaga ggaatttatc cacaggtaaa
    23041 aataatgaaa aaaatcgcta ccgcaatgaa tgtgagtgtt tctgatctct ttacacagga
    23101 tacccctatt aaaaaaaata gacattcaac cccagtaaat ccaaaaattt ataaagaatt
    23161 tattgataat gtaaatcaat atcaacgcct gacaggtgcg acgtatgaaa aaatttctaa
    23221 tatcataggg aagtcaaatt catatattta tgacgttatc gataaacaga gaaagagtac
    23281 acttagtata aaaagtaacg ccagtttaac taaggggtct atgattttaa gacaagaaat
    23341 tgaaaaaatc gagagtggaa agaatcgaat tccctctaaa ttaacctatc aaacaataga
    23401 tagagatagt gaagtggtgt ttagatttaa tggtctaata aaatccgtta atgatttatc
    23461 tgataaagaa ttacagataa ttgtctcaat atttgatgcc ttgaaaattc cagctaagat
    23521 ttcaaaaatt gaaattagag agactaatgt ctttggagga ggaaaatgac aaataccatt
    23581 tacaaaaaat tactcaatgt ttttaaagga ggaaagaaat atgccaaatt gggcagaggg
    23641 gactcttaaa ttaagaggca gacgcgaaaa cgttgcatca gctttaaaag aaatgctatt
    23701 aggaaataaa ggcgcaacac ttgaagaaga atacgatggc actctactaa tatttaaaaa
    23761 cgagtatgat tatttttata taaacggtac aaggcgtgcg tttatttcta gtaaagatat
    23821 tgaaatttgg ttggatgatg attttgtgat tatcgaactt gaagatttcg aacaagcatg
    23881 ggcagcatta gctgacaatt acacagaaat ttctagtaag tttgatgttg atattaaaat
    23941 tttcactttt gaaatgggta tggaatttac acaggaaatt gaaatttcaa aaggtgaaat
    24001 catcaagaat attgtaaacg aaaactttac taactattca tgggatgtgc cttttagtag
    24061 acttggagga tagataatat ggctgattta acatttgcag aattacagcg aaaaatgcaa
    24121 atcgaaaaac aaacgaaaca gggagtgaaa tatccgttta gaaccgcaga ggacataaat
    24181 aataaattta agtctttgga tagcggttgg agtgtatcat ttccagaaga tgacatcatt
    24241 caaaaaggtg acaaactgta ttataaagcg gtagctgttg ctaaaagaga aagtgatggc
    24301 acgattgaaa aagctattgg atgggctaga gaagaagatg taccaatttt tcacacacaa
    24361 aaaggggatg tgaaacagat gcaagatcca caatggacag gtgcggttgg ttcttatgct
    24421 agaaaatatg ctttacaagg tttatttgcc attggaggtg aggatgttga tgagtatcca
    24481 gtagaagaaa gccaagagca aggacagaat aatcagcaac agcaacaaca aggaaatcag
    24541 caagcccaag gacaaaatca agtaaggtat attgacaata ttcagtatca agaaattaac
    24601 gaccttataa atgatattgc aaaaattaaa gggatgccgt tcgatacgct tgctaactat
    24661 gtattatctg aaaaattaaa aggtttacaa gattttcata gagtacaagt tggtgactac
    24721 gaggtattga aaaactattt aactgaacaa ctagcaaagg caaaagcaaa gagaggtaat
    24781 taaacatggt aaaagatgta actaatagct tgacagaaat caaggtggat tttcaacctg
    24841 cagtaattaa tgttgaccgt gaggcgatcg aggtacaagt agccacagcc attgcacagt
    24901 atagcggtcg cgaggttact gttgataatt acaaagaagt ttatgaagag cgaacccgct
    24961 ttaataagct gattgggggc ttagacactc aacgcaaaga ttttaaccga caaatcaatg
    25021 agccggcaaa agactttgat aagtgggtca aagaaaaagt catcaagcct attgaggccg
    25081 taacagatgc tatgtcagca ggacttaatg cgattgatga acatgaacga ttgatgcgcg
    25141 tggatgtcgt gcgtgctaca tttgaggata agtgtatggt cgcagggatt gaaaaatcca
    25201 cattcgctga caaatacgat gagtacagcc tcaagaaata ttttaaaaca ggcaagtatg
    25261 agctgaaaaa gacaacactt gatgaaatgg atggcttagt actttcagaa tttgatgccc
    25321 tggaagaata caaggctaac aagcaagcta tccaagagca agctcaagag tatgatttgc
    25381 cagctgatag ctatatcaga catcttgaag atggtaagag ccttgttgat atcctcaaga
    25441 tgatgaaaac tgaccgagat gctgagattg cacgcaaaga gcagaaagag gctcaagaaa
    25501 aagcaaaagc tgaacgactt gaagaaattg ctcaatcggc caagaaaaat gctaatgcga
    25561 atatcaaggc ttacgatgcc gaaacaggcg agattttgga acagggtaca attacaccag
    25621 aacctcaaaa caatgcgcga gaggtggcaa aatttgagcc tagcgagcct ttggtcaaat
    25681 tagtacgtct tgaattgcac ggtggtttag aacagtggga aaatacacaa gaatattttg
    25741 aggataactt tatcggtttt gaaactttgg aggattaagt agaataagga gtcagaccta
    25801 tgagatgttt ttatgtcagc ggtaaaattg cagatcttga tttggggtca gaaatcaatg
    25861 cagaaaattc atttatggcc gctattgagt ttgtgaaacg atataccgac ttattaaagt
    25921 ttgattcaaa tgaaatcaag gtatcagaag tagaggaggt gcaaaatgat aaataacgtt
    25981 gttttagtag ggcgacttac aagagatgcc gaactgagat acacgcaatc taatattgcg
    26041 gttgctacgt ttactcttgc tgtaaaccgt ccatttaaaa acgaggctgg agagcgtgag
    26101 gctgatttta tcaattgcgt tatctggaga cagtcagctg aaaatcttgc taattgggct
    26161 aaaaaaggct ctcttatcgg agttacagga gtaattcaaa cacgtagcta tgataaccag
    26221 caaggtcaac gtgtttatgt cacagaagtt gttgccagta attttcaact gttggaaagt
    26281 cgtaacagtc agcaaaataa tcaaggtcat caagaccatc atggcggtta tcagcagcag
    26341 ggctacagta atcaaggcag ttctttccaa aatggaaata gttacgggca acaaggcagt
    26401 ttcgttgagg ggaacacaac aaatctagtt cctgatttca cccgtgataa caatccattt
    26461 ggcagaccca caaatccatt ggatattagt gatgatgatt taccgtttta gcgaaagggg
    26521 atattctagt taagttatga aattcttaga cttatttgct ggcattggag gttttaggat
    26581 cggcatggaa tcagccgggc atgaatgtat aggattttgt gaaatagaca aattcgctag
    26641 agctagttat aaagctatac acgatacgaa aggagaaatt gaattacatg acatcacaac
    26701 agtatcagat gacactattc gaggaatcgg aagtgtggac attatctgtg gaggatttcc
    26761 gtgccaagct ttctcaattg caggaaacag acgaggtttt gaagatacac gaggaacttt
    26821 gttctttgaa attgctaggt tcgcatctat tctcagacct aaatatctat tccttgagaa
    26881 tgttaaagga ttgctcaatc acgaaaatgg agttacattc gagaccatta tctcaacctt
    26941 ggatgaactg gggtacaacg tggaatggca agtgcttaac agcaagaatt tcggagtccc
    27001 ccaaaatcgg gaacgagtgt tcattattgg acattctaga aaaagaggca ccagaagagt
    27061 ttttcctatc ggaagagcaa atagagaatt tgattttgaa ccaaaaataa agattgttgg
    27121 taacactaaa aatccgaacg ggacaagtca agggactggg agcgttgttt acgactcaaa
    27181 cggtttagtc ggtacgcttt gcgctagaga ttataaagag ccaaaacaag tagctatacc
    27241 agtattgaca cctgatagag taaataaacg acagaatggt agacgtttaa aaaaaaacgg
    27301 tgagcctatg tttacgctga cggcacaaga ccgtcatggg attttaatta aagaagcaac
    27361 aaaaaaaggt tatgcagagg ctacagttgg tgatagtgta aacctatctc atccaaactc
    27421 taaaacaagg cgaggtagag ttggtaatca gatagcaaat actctcttaa ctggagagag
    27481 tcaaggtgtg gttgagcctg attttaggat tagaaagctg acacctagag aatgctggag
    27541 gttacaaggt tttcctgatt gggcttttga taaggcgcag gaggtcaact ctaacagtca
    27601 attatacaag caagcaggca atagcgtaac agtaaacgtt atttctgcaa tagcacaggg
    27661 gttaggagga aattaaccct atttaaacca atttgaaaag gaaacagaaa atgacaaaaa
    27721 ttgaaatcgt tatggtactt acaactttga tgtctatcac atgggcagcg attgttacaa
    27781 ttcacactat gcaagctatc aaaaagcaca aggcaaaagt ggattattat cagaaaccac
    27841 aagtgcaatg tgagattgca cgtcatgtac ttaaaaacaa atggtactca gatggtgggg
    27901 aggtgtttag atgaaagtat ttgatggcgc taaaatgcgt actatccgta aagaggcaaa
    27961 gcttactcag tatgatcttg cccctatggt tggcattagt caagatcgct taaagcagtt
    28021 acaaaaactt tttaagtggg gagtttgtga tgaaatttga acttatcaat gaccactttg
    28081 aaaatgctaa gcgatacaac ataccgaggg cgcaacttat cattgctgat attccttaca
    28141 acctaggaaa taacgcttat gcttctgatc caagatggta tgagaatggt gataacaaaa
    28201 acggtgagag taagttggct ggaaaatcgt tttttgacac agacaatgat tttaaaatca
    28261 ataatttctt tgatttttgc agccgtttgc ttaaaaaaga gccaaaagaa aaagggaaag
    28321 cgcctgctat gatcgtcttt catgcctggc aacagcgaga catgattata gaatgtggta
    28381 aaaagcatgg ttttaataat gcttatccgc tctatttcac aaagaaatca agccctcaag
    28441 cgctaaaggc caatatgaaa attgttggtg cggttgaaga ggcaacggta ttatatcgtg
    28501 ataaactccc taaatttaac aatggtgggg ctatgatact caatcatgcc ccgtgggaaa
    28561 aagatcgctc ttacccagtt atccacccta cgcaaaaacc gataccagtt ttgaaacgat
    28621 tgattgaaat ttttacagat gagggcgatg ttgtcattga tcccgtagca ggttctggat
    28681 caactctaag ggctgctatt gagatgaata ggtcagccta tggatttgaa attaagaaag
    28741 atttctataa ggctgcacaa gagaaaatgc tatcgtcatt tcaaattagc ttaatttaaa
    28801 gcaggaggac aatatggata aaaaacttat tgggttagac ctaacccaca ttgcagatgg
    28861 aggattacag gagaaactag acaaagagct tgaaaaagtc tttgataaca tccttgacct
    28921 aaatacagat gcgaaagcaa aacgaaaagt gactatcaca cttacaatgt cagcaaatga
    28981 agagcgtaca gttgttgata ctaccatgga ggtgaaatca aaatttgcgc ctcaaaatgg
    29041 agtagctaca acaattctta ttgggcgtga ttttgataca ggacaagtac atgctaatga
    29101 gttaaaaagt acagtacctg gtcaaatgta ctttgatgaa aacggagaaa ttctgacgga
    29161 tattgggcaa ccagtagcag aaattgaaca acaagcagaa acaaaaccag atattattga
    29221 tttcaacaaa aagaaagtag gtaactaata tgacaacaga aaatcttaaa tcagtattgg
    29281 aatacgtagt agaactaaat gagcatggtt tggaaatttt aacagctgca gatggaacag
    29341 agtattatga tgccaacaaa ttcaacctca aagaacttga ccctaaacac tatcctaaaa
    29401 ctctggagct atcaaccttg acaagccttg ttgactatct caaaacagac ctaaacaatt
    29461 tgaaaaacca acgcttgatt gtagcagttg agaaaaacga tgaggtttgt gtgtggtctg
    29521 aaaatgatga gatcgaacat cgcacattgc ttgttgatgt taaggcacgc atcccagagt
    29581 tatcttttgg ccgtttccta tcattggaac agttcaatat catgttgcaa tcaaacttta
    29641 ttgacgataa cgatcgtggc acattgctag aatttgctag cgcattgaaa attgagaatg
    29701 gggctgaaat tgaagataat ggagtatctc aagtagcaac agttaaaaca ggggtggcaa
    29761 gccttgctaa aggcaaagca cctaatccgg ttacattgcg cccatatcgt acatttagcg
    29821 aggttgagca accggcaagc ctatttgtct ttaggattga taagcaagcc aatatggctt
    29881 tatttgaggc agatggaaag cattgggtag ctgatgcagt aggaaacatt gcggcctatc
    29941 taaaagagca actagcagac caaaaacata tcacagtatt agcataagaa agtggaaatt
    30001 aaaatgagtg attacaaaca acggatgatt gaagaataca aacaattaaa agagcgcacc
    30061 aataagttaa gtttgatgat tagtaactat tacgtaggaa cacttgattt taaactaaaa
    30121 tgtcctattg agttacttga aactcaacac tatacaatgt gtgcatatct caagatcctt
    30181 gaacagcgtg cagaaattga aaacattgag ttttaaggta atcaaaatga aatttgagtt
    30241 ttctttgcct cggaatacta agctaaaatc tctaaacatg gttatcaata gtaatgacag
    30301 acaacatcaa acagataagg ctaaagttac taaacgcatt agagcttttg cttattggca
    30361 tacatcgatg aacaaggata aagggagggc tgcttttagc ccctccaacc cttgtgaggt
    30421 tacagttaca atttacagcc ctactaagtc aaaacttgac ccacctaatt tatatccaac
    30481 agtcaaggct atcattgatg gcatgactga tgcaggtatt tggacagatg ataatcataa
    30541 ggttatcaaa aagttatcct ttgtttatgg tggattaagc gaggaaaaag ggcattatag
    30601 attagagttt gatatagagg aggtttaaag tgattgaagt taatataaaa ttcgataatt
    30661 ttgaagcgca tggcttttac caagatgata ctaaactagg aaaaattaga gatgcaatca
    30721 tatctcaaat gaataatggg catgtggttg ttttagggga agatagaggt attctattaa
    30781 atcctaaagt tataaaaagt gtacaattta aggttgtaga agataaccag atctaatcgt
    30841 tttttgacct ttagagaatc tgagttattt tgaggagttg gaagatgatg gaagagttaa
    30901 agcaaaaagt taatgaagta tacaactgga cggtagaaga cgggaagccg caacctccca
    30961 agcaagattt accacaagcg gtgaaagacc gggcggacta tttttgggaa atggcagaag
    31021 atggtatgac gtttatggga gcgatggaat gcatcttcgc tgatgaaaag cctacagact
    31081 atgatttggg agctactaag gattggttgc caaaatctaa ggagtttgat gattgggttg
    31141 gctattcgcc aagcatggct caggtagtta ttgcagttta tttgatttat agaggaaact
    31201 aagatgaatg tacagcgatt aattgagaag tataaaaaac ttgagggtgt atgggatgct
    31261 gaaggagcag agctagctcg tcaaattttt ctagaagact tgaaacaact agacgaacaa
    31321 aaaccagtca aagtaccgca gtgtgtggcg gaatatatag aatttaaaaa gaaaaacaat
    31381 tttcatgttt acggtgcaat gagagtaatt gaagatcatt atgataagaa agttcctgat
    31441 tggttttacg aaaataacat cgaaaaattc tgtcttgctt ggcttaacgg ctacgaggtc
    31501 gaaaaagaga agcggtattt tgttaagatt aaagggaata ttaaaggaaa tatgttggtt
    31561 tatggagaac ttttgaaaag gtatttcttt acaaaaaact ttagtttaga caatgttata
    31621 tattcccaca ctcgtaaaga actagaagac gcaaacttcg gctgggtgtt tgattgccca
    31681 ggtattgaga ttgaggaggt ggaataaatg acaaaatttg ttaaaataca atcttgttat
    31741 agaggacata ctgatgatga gcttatcaac atagatgata ttagtcgcct ctgtctaggc
    31801 cctaatatct tgtttttaag aacaccttac aatttaggag aacatcatat ttctatcact
    31861 caaaattcag tagataaact tttgaaagta ttggatatta ttggggagga cggcaaatga
    31921 gaccttgtaa atatccattt ttgggaagaa gaaaaaaaca agaaacgccg tcgccaatat
    31981 tttctgcacg accaattttt gacgaagttc caattgtaga agaagttaag gttgagttcg
    32041 gagttgaagc tagtatgggg cgcatatatc cagaaacgtt aatacattta gatatttctg
    32101 gatacggaaa tagagtgcat tcagtacatc gcttccccgg tattttactg agtgttggtg
    32161 agtcaatcca actaaagatg cttttctata aaagacttag aaattttact acagatcgtt
    32221 tcttgacgtt tagagaatct gattggaagt tctttatccg ggacctggtc aacgaattta
    32281 agcattaaaa aaagccaaga cactctctgc ctcagctaat agttctcgca aagactatta
    32341 tatcacaaag gagacagaga gtgaacaagg ctaaagagct cttgaaagag ttgcaggatc
    32401 tggacatgga catccaaagc cgtatagacg aaattaacga gcttgaggca ggtttgctct
    32461 caagccctaa atggtccgag gttaaagtcc aaggtggaca gactagaaaa gttgatgatg
    32521 tctatactca gttggtagtg atgaaagagg ctatagagca ggatactaaa gaggtcatta
    32581 acagaaaact tgaattaggt agaatgatca ataggcttaa aaatccaaag tacagggcag
    32641 tattaagaat gacttacatc aacaaaggca ccgctgatag cgtttgttat gatttgaata
    32701 tgagtcgtac aacctactac aggttaaaaa atgaggcggt cttagctttg gaagaagtta
    32761 tctaacctca tagtgagctt atgggacttt ttggaacagc acggttctaa aaatctgtta
    32821 gaatggtagt atcaagaatt gaaaagagag gtctcagaat tggtagatgg ttacctgtaa
    32881 tgtcaggggg ctgtaatggc cttggaggtt caaatcctcc cctctccttt gagtgtttgt
    32941 gtcctagaat ggggtaggca gtaggcttag cattcatata tcactcatta acttaaaaat
    33001 ggttgcagta gtgactggac ctcgcatggt tgcgtagcta attatattcc ggataagtta
    33061 taagctagag ggtttgattc cctcagaggt tgtaaagact acaaaaaata aaaatgaagt
    33121 caaaatttaa tacgcacgca aggtagtagt cgccttgcac ttttagggct tagcctagat
    33181 aatctgtggt aactcaggaa aaggatgttt ttaaatctat caaacatcct gccagcaatg
    33241 gtcaatctaa gcaatgtaat cttaactatt tcagttttgg aataggtagg cgaagttaaa
    33301 gcaggaagat tccaacggca aggtgctgag gaaatgcaaa tgtggctgtt tggctgtgaa
    33361 acgagtctat aagaggaaag aggtatttgg ttcgaggtgc aacaagagct taataccata
    33421 tcttacaaaa attgggtgcc tcccaaaagt atgtaagatg agtcgattgt ccgcaaaaca
    33481 atcgataaca agcaggcgct gtgcattttg ttcttcaaaa gagaatgaaa cacatggcga
    33541 tgcgtgtctg tgatagatga aagatgattt ttatatttta aggctattca agatagaaaa
    33601 aactcaaaaa agcaaaagtc atcgcccgtc gtaaacgaaa gtgcacttcg gcaattagat
    33661 tgcctgctca agtctcgcaa ggatgagagt aaagtcaaag agtaaagcag cttagacttt
    33721 tagcggagtc ttcgttaatt gaaaaatggc ttagtagttt gcgatgtgag gagtgattgg
    33781 tctaaccaat cgtgcatgag tgatacaagt aggaatattt gtggacaaga taataaacta
    33841 taagttatca aaagtcactc gtttaaagca gtagtctcat gctagttaat ggatacatgg
    33901 tagacggatt aagtcctgtt tagggaattg aaacgtaggc aggttcgaat cctgtcgttc
    33961 caattgcgat tttaattcgc agagagaggt cttgaaaagg tcgcacatcg tgtggccttt
    34021 tttgattgtt tgaaaggtgg tgatggaaaa ttgaatgaaa gacaaaggcg ttttgcagat
    34081 gagtacatca tctcaggtaa tgcttatcaa tcagctttaa gagcaggata tagtgagaaa
    34141 tatgccaaag caagatcttc tgaattgttg gataatgtcg gaatttctga ttacatcaaa
    34201 aatcgaatgg aggagttgca agatgaaaaa atcttaactc aaaaacaaat acttgtgatg
    34261 ctatcagaaa ttgcgtcggg acaagcgaaa gaaacaatag tagtcacaac aaaagtagct
    34321 gagttgatga ctgatcccgt gactggtaag tctgtaaaag tctacaatga aatccctcaa
    34381 cttgtcgaat acccaacaaa gaacagcgat aggaataaag ctcttgaatt gttaggtaaa
    34441 cgacataaga tgtggacaga caaagtagag gcagacgttt ctggaacggt ggtgtttgca
    34501 aatgagtcag acataccaga ttaaacaaag tgatattgta atcgacctac ctaagacagt
    34561 aggagctggg tacggacagt tctggcgctc aagaaatctt tatcgtgttg taaaaggttc
    34621 ccgtggttcg aagaagtcca agacaaccgc tttgaattat gttatccgtc ttttgaagta
    34681 tccctgggcc aacttgcttg ttattcgtag atactcgaat accaacaagc aatcaactta
    34741 tacggatttt aaatgggcgt gtaatgtgtt gggtgtgact catttgttta aattcaatga
    34801 atctctgcct gaaataacca taaaagcgac tggtcaaaaa atcctattcc gtggtttgga
    34861 tgatgaactc aaaatcacat ctatcacggt cgatgtcggc agtctttgtt gggcatggtt
    34921 tgaggaagca tatcaaattg agactgaaga caagttcagt acagtagttg agtcaatccg
    34981 tggtagccta gatgtacctg atttctttaa acaaatcaca gtcacattta acccgtggaa
    35041 tgagaggcat tggctcaagc gtgtattctt cgatgaagag acgagacggg ctgacacatt
    35101 cgctactaca accacttata aatgcaatga gtggcttgat gaagtcgata tcaaacgcta
    35161 tgaggatttg tatcatacga accccagacg tgctagaatc gtttgtgatg gtgaatgggg
    35221 agttgctgaa ggtttaatct atgagaacgt gaccgtcaag gatttcgata aggatgaatt
    35281 gctacgagat tcagctaata agttatgtat cggtcttgac tttggtttta ctcacgatcc
    35341 aactgctttg tgttgttcgt tgataaatga cacgacgaaa gagatttatg tctttgatga
    35401 ggcgtataaa gtcggattga taaccaaaga agttgcgaag atgataaagg acaaaggtta
    35461 tcatcgctca caaatcattg ctgatagcgc agagtcacgg ctgattgaag agctcaggtc
    35521 agaacatggc atatctagaa taaaagagag tcggaaaggt aaggatagta ttatggcagg
    35581 cgtatcaaaa ttgcaaggat acactattta tgtgcatcca gattgtaaaa acatcatgga
    35641 tgaattttat agttactgct accagcgaga taaagaaggc aactggttga ataaaccaga
    35701 ggataaaaac aaccacttga tggacgcttt gcgttacagc cttcaatgta tcgaaggtgg
    35761 gaaagcaacc gtccgcagac gttctgatta tggtctatag agaggaaaga catgtaccaa
    35821 tatttaacct atccacggga tggatatgat gagggttctt tgaagaaaga cctgatttac
    35881 aaattgataa cgatacatag cactgaaggc tcgcatttga agaagcttaa aagctactat
    35941 ttgggtgagc atgctatctt aaaacacacg agacgcaacg tgaacgcacc gaattacaag
    36001 acggtagcca atcatgccaa ggatatcgca gacacggcta cgggctattt tatgggcaat
    36061 cctatcaagt ataacaatac tgctgacggt gatatcgatg aactacttac agcctttgat
    36121 ggtgctgaga ttgaccaagt agatgctcag aatgctttga atatggctat ctatggtcgt
    36181 gcttacgagt acatctatgc taaagagggt atggctgagt tggattcaac tagtattgat
    36241 ccggagaata ctttcatggt ctacgatgat agtattgagc ggaagccttt gtttgcggtc
    36301 tattactatg aagtaaaaga cgatacgaaa gacactacca agcaccaggc tgaggtcttt
    36361 accgaaaatc tgcactatca catggtgctg agaagtacag attcaggaac aactcagagc
    36421 gaggaggcaa cacctcacaa ccttggtcaa atcccaatta tcgaatatcg caacaatcac
    36481 tttgcaattg gtgactatga gcaacaaatt agcttgatag acgcttataa ttccttgatg
    36541 gggaatcgtg tcaatgataa ggaacaggct gtagagtcta tccttgtctt gtatggcacg
    36601 cagttagcag acactccaga agacgctaag gtagcaatga agattctttc tgaagaaggt
    36661 cttttggaat tgccgggcga tagtgcaagg gctgagttct tgaagaatac gctggacgaa
    36721 agtgctactg aaatcttgcg tacagctctt aaagaggaca tctacacatt tagccatgtg
    36781 cctaatttga ctgatgagaa tttcgcaggg aatacatcag gcgtagccat ggaatttaag
    36841 ctgatgggcc ttgagatgat tactaagacc aaggaagcga actataagcg aggattgcgt
    36901 cagcgtattg cgatttttgc tcattactta ggcatgaagc agattgcttt agagtctcat
    36961 tcaatcgttc cacaattcag tcgtggtttg cctaagaact tgttagaaat ctctcagatc
    37021 gtgaacaacc ttgaaggcaa agtgaccaat agacagctta tttctctctt gccgtttgtg
    37081 gaagaccctg atgctgagct agaagccttg gaagaagaga aaaagaagaa catggaagac
    37141 atgccgatgt ttaaccaaga caacacgaaa cccgaagacg aggtagagga tgaagaatca
    37201 ggagtattgg gcgaagagga aagccaatct gatttaccag cagatggaca aggccgaaaa
    37261 gcaggcagat cagttcgata aggtctatca ggaagccaag acttacttgg ataaggaagt
    37321 caataagatt tttgataagt tccaacgtga ttatggtcta agtcaggtag aagctagaca
    37381 agtcttgaag aacatgaaag acaagaaaaa tctgaatgaa cttcgtaaag tacttgaagc
    37441 gagaccgaat gatccaaata tccaaagact actggctgac ttagatagtc cggcttattc
    37501 tttccgtatg aagcgtctag aacgtttgag tgatgattta gaccgtatgc gtgaatctat
    37561 ctatcattca gaaaagacag gctcagacgc cttttatagc gacctgatga aggatagtta
    37621 ctacaaggct acctttgacc tgcagcagca gacaggacta gcatacggct tttctgggct
    37681 tcctgagagc gagattaaac atctacagtc tttcagttgg gtaggtgacg gaagtacgta
    37741 ctcaacaaac atctggaaga atacagggaa gcttacatca agcataaaag atgaattact
    37801 cataagcctt atgacaggcc gagatacacg agaaactgca caagcaattg ctgagcggtt
    37861 caatgtgggg cagaacgatg caaggcgttt ggttcggaca gaatcagcct tttttcataa
    37921 ccaaatggag ctactcaact atgaggaagc ggatatagag aagtatatct ttgtggccgt
    37981 cttagacaag cgtacatcac gcatttgtca ggagcatgac aatcaggtct atgataggga
    38041 taaggctgtc cctggtgtca attgtccgcc tatgcaccct tggtgtaggt ctactactgt
    38101 cggatacgat gaggacgcag actacagcaa gttgaagcgc agagcaagga atccagagac
    38161 aggtaaagtt gagtacgtgc ctgccgatat gacttataaa gagtggtata gcaagtatgt
    38221 ggatggcaat agagagtcta ttaaacgtaa agcgtttgat aaaactatta aagatggtat
    38281 aatagtaagt gtatcaggga ctacaattgg acacactccg cctggcaaaa taggtttgcc
    38341 taatagtgta gttcagcata atgctacaaa cggagatgtc cttggtagaa cttactatga
    38401 tgctagaggt tttaaaacga aggatgttca ttttacaaac cataaacaac cggcacgtca
    38461 tccttatgga aaaatcggag aacatgctca tgattttgta tttgatgatg aaggtaagtt
    38521 cgttagtagg agtactaggg aattaacaga tgatgaaaga aaggagaatc aagatatatt
    38581 atggcgatat tagatgattt acaagcgtta tatgataatg gatgggacgc ttcttttaat
    38641 tataatggtc aagtatgtgg cattttccct aattctattt atgatattgt tgttattatt
    38701 gcggacaacg aatatagagc atcttctttt gacgatttga tttctttaca gattgaaggg
    38761 aaaactttac cggaaatcat gaacgaagtt gaagtacaat atggctaaag cacctagaga
    38821 aatctaagtg cttttttcgt acccagaaag gattgaggaa tgaaataccg taaaaaacca
    38881 gtagtggtcg aggctgtgca gtggaacggc aataaccata aagaggtaat tgactttgca
    38941 gaaaataaga tttggtttga tgcacttggg aatatatgga ttgctacact tgaaggtgat
    39001 atggtagcta aaaaagggga ttatattatc aaaggtgtgc aaggagaata ttatccatgc
    39061 aagccggata tttttgcaga aacatacgaa aaaacggagg agtaaaatgt taaaaaaagc
    39121 aaaacaattg gcatcgcaag aattttcacg cttggcaggt cgtgaaatca aagaagaaga
    39181 ctgctttgta gtttggttta gtaagaccct gcaaaactgg aaagctcttg ttagtacgaa
    39241 cgcaattaca tcaagcgaac cttgtggaga ttatgcagaa attacgcata acggagataa
    39301 gaatgagact tatgtggatg tttacgccaa ggtttcaaat cgtgccatta aagattagga
    39361 ggtgatccaa catcttgact tgcaggaata gactgctata aattactgta aattgctata
    39421 aaccgtgtca tatttgatgc ggttttctta tgctctaacc gtatggaatc ccgtacggtt
    39481 tttatattgt ccaaactgtg ccgatgacat taaaagctgt actgttccgt cgccggacgt
    39541 aaagcgagat tatcgagtgg cgacgtaatc gctggaggac aattatgtca gaagaaatca
    39601 atgcaactgt atctactgaa tcaactgaga ctgtcgacac tcaagaaaat gttgaaacag
    39661 tgcaagaaga aaagcacgaa cgaactttca ctcgtgctga aatcggtaag atgctatctg
    39721 ccgagcgctc taaatgggaa gctgagcaag aagccaagga aaacgaagct aagaagcttg
    39781 ctaagatgaa cgctgatgaa aaacagaaat atcagttgga tcagcgtgag caagaactag
    39841 ctgaccgtga aaaggctatt gctcgtaagg aattgaccgc agaggctaaa gcaatgctaa
    39901 gtgaacgtga cttacctgtt gagttagtaa atgtagtcga tttgacaaac gcagagacgg
    39961 tatctgaatc tatcacctct atccaaaaag catgggaaga gtcagttcag aagggagtct
    40021 ctgaacgtat gaaaggtagt gcacctatca aaaatgcaca aacagtccag caagaagtca
    40081 cggaaaaatg gcgtaaagac ttcttgtaat aaaagaaaag aggaaaaata aatggcattt
    40141 gaagaattaa acacagcaga atcacgcaag aaacatcttg ggattattga ggatgtactt
    40201 gcagtaaatt catattcaac accacttgtg acatcaagcg acgcagtaac cttgcaaggt
    40261 cgctctttta cagtagcaac tggtaacaca acagagttga aagactacaa acgtaacaaa
    40321 gacaatgaat ttgatcacgt tgaagttgaa gaaaaggttt atacccttga tgaagaaaaa
    40381 tactggggtc gtttcgtaga tcaattggac gaacgtgact ctaatggtca agtgaatatc
    40441 aattatgtta ttgcccgtca ggctgcagaa gtagtcgctc catatcttga tgaactacgt
    40501 tttggtgcag cacttggaaa tgtaagtgac aatgttgcca tgggtaaaac agcaggagcg
    40561 aacaacgctt ataatgcggt tcttgatgtg tctgagaaac ttgatgagct tggaattaca
    40621 aaagaacgct tgctcttcgt cactccaagt ttctacaaag cgatcaagtc tgaaatcgtt
    40681 cgtctaccac atggtgacgc agataagaaa gtccttggaa aaggatatgt tggtgaattg
    40741 gatgattaca cagtctataa ggttccttct aaattcctga aaggtgttaa tgcccttgct
    40801 actgctccag gtgttgttac atctccagta caagtagata atactaagta caacgataac
    40861 attccagggc gatttggcga attggtagag caattgcttt acactggtgc atttgttctt
    40921 gaacacttca agaaatacat catcacaatt gcagattcta agcctgctgc taaaccatct
    40981 actcaaggta aagttgtaaa ccgtgctaaa gcgtggaaaa ctggaacaac ctacaaagaa
    41041 ggtgacacag ttactcatgc cgataaagtc tatgtagcgg ttaaggatat ttctaattca
    41101 tcaaccgcac cagacacaga tacaactaac tggaaagaaa aaactggtaa gaaataggtc
    41161 cgagttatga aatttaaaat caaacaagat ttctatgatt gggaatcaaa tgtgaaacga
    41221 ctggcaggag aggaacttga gattactgag gagcgctatg ctgagctggc tgacaatatt
    41281 gccagcaacg gtgtcgctat ctcagatgtt cttgaggaaa tctttcctaa acctgagttc
    41341 ttagaagagg attgatatgt ctatagagtt gctgaagaaa ttaacaggcg aagaagatac
    41401 tcagcttctc atgttgctcc aaacaagagc tacaaatctt atcttgtcag agactaatcg
    41461 cacatctttg acacctgctt taagtctctt aatacctgag gttgctatcg agctccacaa
    41521 ccgctcagga gcggaaggag agcgttctag aaccgaaggt ggtatagcag tagtctacgg
    41581 agaaaacggc ctgtctacgg gccttttaca gcgtatacgt atgcatagac tagcaagggt
    41641 ggcaggccat gtttttgaag cagagtagac tgaaacctta tccaatgcga cggtttgaaa
    41701 agactgtcac tgaggaaggt gtcgcaaaag aaggatatgt caaggaagct gagacaatcc
    41761 gtcttgagtt gtggccagct agtagtaaac tacagtctga attgtatggc gagcgtgtca
    41821 atgatatttt gaacgcaaat gccaacaagt cagctacaat caaagtgaaa gatggtgtgt
    41881 gtatcgatag ccagacagaa gtgactcaca gggttatttc caaaaaggtc tacacacatc
    41941 atcaagtttt ggagttagag cgtgtcaggg ctactagggg cagataggct catagctaaa
    42001 tgtagacgat tggctagtaa aaaaactggc gaggatatcg tcttacgtgc ggtacacaat
    42061 gctactataa aggttgtcca agcagatgca agaagactcg caccagcgag agatggagag
    42121 cttataacta gtatcaaaac tagggcaaaa atggacggag ataaggctat aggcgaggtt
    42181 tacaccaatc ttaaatacgc tccttacgtt gagtttggga cagggccaat aggacaagct
    42241 agccattcgg gtatctctcc agaggtcagc gtcacttata agtctagtcc gtggtatgtg
    42301 catgaagacc aaatcaatgt aggaccttac cattttcaaa aaattgggga gttctacaag
    42361 atgtatggtc aacctgccca gccttatctt tatccagctt tgagagacaa tcaagagcgt
    42421 gtgtctaaga atatttcgaa ttatgtccgt agaaagataa gagaacaaat aaaatgatta
    42481 atatcaagcc tgttatttat aaagaattgc aaaaggttgc agataatgtg accgatactt
    42541 atcctagcga ttgggagaat ttcccagtcg ttattttttt agaagaacag aacaagccgg
    42601 gtgattggtt tgatgaccag gaacaaaaat catctatccg ctataaggtg gatatctttg
    42661 atgataccag cactagtgag ttagctgtta aaatcaatca gatttttgag tctttaggtt
    42721 tgcgaagaac tgactgccaa gacgtgccag acccgtctca tttgagacat aaggtcatgc
    42781 gttttgaagg tgtcgttgat ttagactcag agcttgtttt tcaatttaga atggagaatt
    42841 aaacatgtta gcaaatggaa ttaaattggc ctttagtgaa acgaaaggca attatcagaa
    42901 tcttgtaggg cttaaggaag tacctgaatt tggtatcgag cccgaaaaag tagaaaatac
    42961 tactcttgca gatacggtga agaagtacga gtttggtatc ggggacgcag gagaacttga
    43021 gtacaagttc gcttataata attcaagcgc aacagctcct taccgtgtat tacgtaaggc
    43081 agcagatggc aagaaaaaac tctactttga acaaacctac ccagacggta ctaaggtcaa
    43141 ttttgaaggt caagtatctg taaaacttgg cggtggcggt gtcaatgccg ttatcgagtt
    43201 cacacttaag attgctttgc agtctaatct tacatttact gatggtgttg gaggttaatt
    43261 aaatggcgtt aaaatacaca acttggaaag ttactgacga aaaagagttg aagctacgtt
    43321 tgacatctca tcaagctgca actgtggaag aaaaaatcgg catgaacttg ctgaagattt
    43381 tcatgcctga agctggcgaa gagttcactt taccgccttt gaaagttatg ttgttgttag
    43441 ttcacggcgc cttgcagcag tatgaacatg ggtattcttt tgaggatgtc tatgatttat
    43501 acgatgaata cgtggacaat ggtggagacc aaacaacatt catgacagag gttttaatgc
    43561 cactctttga agtatcgggt tttactccac gaggaagcaa ggacaagaaa acttccaaga
    43621 agaaaatgac agtagtcaag taatcttaac ggtaactcag attattgaga ggctttatcc
    43681 tatgtttttg gacatcgggg gcaagcctct tgatttttgg gatttgacgg tgcttgaaat
    43741 cagggaaatg atagaaagct acaaccgtgt caaaatccaa gagcgtaaag agaagattat
    43801 tgactcgtac atactttcgc gaatgataac taatcatgtt tccttattac tgtccaatga
    43861 cgctaagatt gttgagcttt gggaatatgc gcctgagttg tttgtagaag aacagcaagc
    43921 agtagaacag gaacgacaga aacaagcact tttgttgcat aaggaacgga tgcgtgaatt
    43981 tgcagagaga cataatcgaa aaaggaagga ggaagtaaat ggcaactctt gatgaattga
    44041 aagtcatgat tgacgctgag atagcgcctt tcaggaagaa gatgaaagaa gtcgagaatc
    44101 aggtcaaagg aacatctgac caagtgaaga atgccactgc caaagttcgt gaacagtcga
    44161 actctatcgg tagtgcgttt ggtaagctag ctaagttcgc tggttttgca atccttggta
    44221 agaaattact tgatgttggg atgtattcaa cgcagactgc tcttgaagtg tcagcggcta
    44281 tgaaccaaat caagcgccag atgggcgaga gttcgcaatc tttcttaaaa tgggttaacg
    44341 ataacgccaa cgctatgaac atgggtgtgg gtgaggctac taactacggt gcggtctact
    44401 caaacttatt ttctgggttt atcaaagata ccaacaagct aagcgcctat accgctaaga
    44461 tgttgcagac atcggcagtg gttgctgaag gttcagggcg cacgattaca gacgttatgg
    44521 agcggattcg ctcaggtttg ctagggaaca ccgaagcaat tgaggaccta ggaatcaacg
    44581 tcaatgtggc tatgattgag tctactgaag cctttaagaa gttcgcaaac ggacagagct
    44641 ggcaacagtt ggattaccaa acccagcaac aaatccgcct tatggctatt ctggaacagg
    44701 ctacagccaa gtatgggaat accttgtcta attctgtaaa tggtcgtatc agcctattta
    44761 agtcgctgat gaaggacgca gcattgaacc ttggtaactc tatgttaccg attatcaatg
    44821 ccattatgcc tgtcttgaac tcttttgcga tggtcttgaa gaacgttact gctaaactcg
    44881 ctgagtttat cgctttgatg ttcaacaaga aagcaacagt gaaagatggt gttggtggag
    44941 cagttggaga catgggtaac gccatgaaag acgctgcagg cggagcagga gaccttgctg
    45001 atgcagtaga cgacgctgga gattcagcag gaggacttgc tgacaatctt ggagactccg
    45061 ccaaaaacgc taagaaagct gctaaagagt tgctaggtct tttgggattt gatgagatta
    45121 acatcttgca aaaaccaaaa gatgacgacg caggcggttc tggaggcggt ggcaaaggtg
    45181 gtaaaggaaa gggaggcggt ggcggacctt tcaaagacat cttgccagaa gtcgagttga
    45241 ccgacatgga caacaaattc aagagcattt ttgatggtct tggagataag ctcaaagggt
    45301 tgtttgacct cttcaagaaa ggttttgatg cagcatttag accagaaggt ataaaacgca
    45361 ttaagactgc cttagaccaa atagctaaga caatgggaga aatcgccact gacccaaggg
    45421 ttgtgaatgc ctttaaccga atggctgaga aaattgctta tgctttaggg caagtgacag
    45481 gctcaataac cactatcggg ctaggtatcg gtgttttcct tgccgaaagt attgcaaatg
    45541 gccttggaag gcaaaaagaa cgcattatca gggcgctagt cgctttgttt gataatgttg
    45601 gtaacctttc cgaggcagta ggaaacatag ctcaggactt ttctagtgct ttctacgacg
    45661 tcattacctc aactggtgcg gttcgtatcg gtagcgctat tgtgtcaact ctgttgagtt
    45721 tgacatctac cattgttgaa gttggtagta aattagcagg aagtttgttt aaaggttttg
    45781 aaaaagtcgt tgtgacaagc gctcctaaaa tttcatcagt cttccaaagt ttattagata
    45841 ctgttgcgcc tgtatttgag agcattgaaa ggtctgttaa caaatttggc gatggcttaa
    45901 gtcgtgttta tgatgaacat gtagtccctg ctattaactc tattgctaat gcttttaatg
    45961 ggctaattga cattattcag attctctggg agaattcctg gcaacctttt gctgagtttt
    46021 tatcaggagt attcggtgtt agtattgaag gaatttcaga tttattagga ggtggccttt
    46081 tagccacttt gggactattg gcggatgcta ttaagttagt ggcagatggt ttcaccgttt
    46141 tttctgactg gtgtaaagaa aacaaagaac ctatcgtagc tttgataaca acttggcaaa
    46201 cgattaattt cttatcatgg gcagaacaag ctggaggact tgcaggagca ttcagcttgt
    46261 taggtagtaa gatctcttcg attgttggag ggattaagaa tctaggtctt gctattaaag
    46321 cattgacatt tgataagttg gtcagttttg ctgaaacaat ctatttgaac accttatatg
    46381 caaaagattt tgtggtcaat tcaggtaaaa caattgcaca gctaggaaaa actgctttag
    46441 aacttggtaa atcagctcta gcatggactg ctcatgcagc gaaaatggga ttagcaaccg
    46501 cggcggaatt tgcacattct gttgcagcag gagtcgctac agctgcaaca tgggctttta
    46561 atgcagcgtt agcagttttg acaagtccaa taacatggat tattgcagca atcgcagctt
    46621 tgattgctat tggtgttttg ctctatcaaa actgggacac tgttgttgag tttgctaaaa
    46681 ctgcatggca aggactatgt gattttatca gtggtatttg tcaagcgatt ggcgaatttt
    46741 tcagcggtct atggacgaaa ctacaagaaa tctttgagcc gataggtcaa tggtttggcg
    46801 agaaattcca gcaagcatgg gacgccattg taaacatatt ctctggtatc ggagagtggt
    46861 tctctggtgt attccaaggt gcatgggacg ctatcgttaa tatcttcaca ccaatcggct
    46921 catggttcgg acaacgttgg gcagatgtga ctagtgcgtt ggctaatatc ggggcatggt
    46981 ttactgacat gttccaaaaa gcatggactg gcttaacaaa catctttagc aaactaggtt
    47041 cttggtttgg cgagagatgg aacgatgtta caagtgcact ttccaaagta gcaagctggt
    47101 ttggcgatat attcggaaaa gcttttgacg ctgttaaaaa tgcctttagc tctatcggag
    47161 acttctttaa aggcgtttgg gatactgtca aaagtatctt cgttaatgct ggtcagatgg
    47221 tcggcgaggc agtaggtgga gcgtttaaga gtgcggttaa tgcggttctt ggaacgattg
    47281 aaaatgtagt caatggcttc atcggaatga ttaatggagt tttaggcgtt gtcagaaact
    47341 tacctggtct aggatgggtt ggtagtgtaa gcacagttag cctccctcgt cttgcccgtg
    47401 gtggtatcgt cgatagtcca acaatcgcca tgattggtga agctggtaaa gaggcggtcg
    47461 taccacttga aaatacagga tttatccaaa cacttggacg agtagtcagc agtgcggtag
    47521 taaatgccat ggctggtgtt agtccacaag gtggattctc tggcgacggc gacatcgtta
    47581 tccaaatcgc aggccatgag ttcggacggg tagccatcca agaaataaac aaggaacatg
    47641 aacgagcagg tcaaaccttg ctcaagattt aggaggttaa atggcacaat tgacaatcaa
    47701 tggggtggct gtgaagcctc ccaaatattt tcaagtcggt attcaagata tcgatggaga
    47761 gacagggcgt aatgccaatg gcgacatgat gcgtgaccgt atcacgacca aacgcaaact
    47821 agactgtgaa tggggtatga tgactcaggg agaattaagt cagcttttac atgctgtatc
    47881 atctgaattt tttgaggtat cttatccaga ccccatggat ggccaagtca caaagacttt
    47941 ctatgtcggt gataggacag ctcctagcta tacctttact gagaagttta aaccttggtc
    48001 tggcgctaaa tttaatctgg tagagaggta agaaaatgga cgctttaact agacgacaat
    48061 ttgacagagc catgtttgcc aaggaaagga cgctggctat tcgtgttggt gattatgctt
    48121 cacgggatat caaagaggct agttttgagt atggctacat taagggcgat acttataagc
    48181 ctggtggaac ctgcgctggt agcggtaaaa ttacctttac cagtatcatt accacgttca
    48241 ataagctgga taccctgcac cctgagattg gtctactggt tggggatacc taccagtggg
    48301 tcaagatggg ggaatacttc atcaacgata ttgagattga ccgaaaccga aacactacca
    48361 cgcttgaact tatggacggt atgtttaagc tcaatcgtga gtacgtgacg gacttgcatt
    48421 tcccagctga agtacgagag gttattcagg aaatctgcct gaaaacaggc attgagttag
    48481 cggatgacta tttcggaatc agcgcgatgc gttatcatat tgagcaagtt cctgagggca
    48541 agaaactttc ctttagggat atgctgagcg ctatgactca gatgattggg atgtcttgtt
    48601 tcttcaacag agaaggcaag atggaaatcc gtgatttgac tgagtccaat atcacgatca
    48661 acgctgacag ttacttcttg catggcttga ccaagagtga gattgagtat cagatagctg
    48721 gtatcacttg taagacggac aagaagtctc tgacggtcgg tatgaagaca ggccggtctt
    48781 tggaactgga caatgtcttc atgacccaga gcgctttaaa tgacctgtat tacaaactga
    48841 aaaacctaac ttactatccg tataatctca actaccaagg gcatttactg cttgaggttg
    48901 ggcagtgggt aaccattcag accaacaaga aagagacttt taaagttccc gtgttaagtc
    48961 agagctttac ctttaaaggt ggtctgagag gtcgtatcag tgcagatagt aaggctggaa
    49021 acgataccca gtattcttac gagggtacga ttaccaagca gataaagcaa caagatggca
    49081 ttgaagcgaa aatccaagcg cagattgaag caacagataa agattttgac caaaaggtcg
    49141 acaaaatcaa aaaagacttt aacgatcaag tagaactggc caaagccaga gctgaagaag
    49201 tcaagagaga actgtctgac actatcaatc agcgatttaa tagctttgac aacgggccat
    49261 tgaaagaagc taagcgcagg gctgaagaag ccttgagaaa cgctggcgca agcagcttac
    49321 tcgctcagga agcgaagcgg attgggctgg attctgttgc tagacttgaa gcgtttaagt
    49381 cgcagactac gagcgctcag atggctttgt caggtgactt ggatgttcta aaacgaacta
    49441 tcgcaaacga tattcgaccg aagcaagcac aggctgaagc tgagattgcc aagcaagttg
    49501 aagcacttag ccggactaag aatgaactgt ctggcgcaag taccctactt gcacaggaag
    49561 ctaagcggat tgagctggat tctgttgcta gacttgaagc gtttaagtcg cagactacga
    49621 gcgctcagac ggctttgtca ggtgacttgg atgttctaaa acgaactatc gcaaacgata
    49681 ttcgaccgaa gcaagcacag gctgaagctg agattgccaa gcaagttgaa gtacttagtc
    49741 ggactaagaa tgaactgtct ggcgtgaagt cagcgcaagc gacgtatgag gagacgacga
    49801 ctcgtagact gtcagaactg accaacttgg ccaatggtaa agccagcaag tcagaactca
    49861 cgcaaacagc tgaggagcta gctagtaaga tagcgagtgt gcaggcatcc ggtcgaaatc
    49921 tattcttgaa ctcactattc aagcaggata ttccaaaaac aggaatttgg acaacgagta
    49981 catatacggc tactatcgat agcgaaagta agtatcttgg acacaaggct cttaaaatta
    50041 taggtctgaa tccatctggc cgtgatggag gtaatcccaa ggttacttat ccagctctgg
    50101 gtcaattcgg gaaagtaatt cccggaagta cgactaatca agatgtaacc attagttttt
    50161 atgctaaggc aaataaaaat ggaataatgc taagatctcg attagggaat atcggatata
    50221 aaactggaaa tgtgacattg tcgacagaaa ttaagcgata tgttgtccat attccaaaag
    50281 gttggacaaa cgaatccaag cggaccacaa atgaatggtt gttcaatttc aaccaagaag
    50341 gaaccgtttg gatttggatg ccgaagtttg aaataagcga tgtagatact tcttattcag
    50401 aagctcctga agatatagaa ggtcagattt caacagttga atccaacttc aaacaacgag
    50461 ccaactcgct cgaagctggt gtaagccgtc tgactgaagg ccttagaacc aaagccgata
    50521 tcagctcatt caacgtggct gctgaaaata tccggcaatc tgtgaagagt cttgagacag
    50581 acacgcagaa caaactaaat cagaagttga gtcaggctga atttgaggtg cgagctggct
    50641 ctatccgtca ggaaatcttg aacgcaacca agaataaagc cagcaagtca gaactcacgc
    50701 aaacagctga ggagctctct agtaagatag cgagtgtgca ggtcgggggt aggaattata
    50761 tccggggtac aaagcgcatg atgctagcca gaggattgtg ggcatcaggt acttttagac
    50821 cgtcaggcgc tgggacggca aagacgattg atgtatcaga cagttcagta actggctttg
    50881 ataaagcgat acgattgact tcaagcaatg ctagagacca aataggtatt gctcaagatg
    50941 gattttatat ctcgcaaggc acatacacga tgtcttgttg ggtcaaaggc agaagaggtc
    51001 aaaaggtcaa gctacaaact tattggcaag ccaatgataa ttcgggtatt tcgcccatct
    51061 ttacattaaa ggatgaaaat tggacaaagc tatcgtttac tagcgctaga aatagggctg
    51121 gagtcgcatc aattggctat gtgtatctcg taaatgctga ggtcggagaa tatttagatg
    51181 ttcttgcgcc ccagctggaa gacggaagtt tggcaacaag ctcaaaagaa gctcctgaag
    51241 atatagaagg tcagatttca acagttgaat cgaccttcaa gcagcgtgct gattcactcg
    51301 aagctggtgt aagccgtctg actgaaggcc ttagaaccaa agccgatatc agctcattca
    51361 acgtggctgc tgaaaatatc cggcaatctg tgaagagtct tgagacagac acgcagaaca
    51421 agctaaatca gaagttgagt caggctgaat ttgaggtgcg agccggctct atccgtcagg
    51481 aaatcctgaa cgcaaccaag gataaagcca gcaagtcaga actcacgcag acagctgagg
    51541 agctggctag taagatagcg agtgtgcagg catccggtcg aaatctattc ttgaactcac
    51601 tattcaagca ggatatttca aaaacaggaa tttggacaac gagtacatat acggct
//
LOCUS       DAVKWZ010000007        50783 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000007 DAVKWZ010000000
VERSION     DAVKWZ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 50783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50783
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.007"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(<1..516)
                     /locus_tag="VW854_000450"
     CDS             complement(<1..516)
                     /locus_tag="VW854_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000906797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0261740.1"
                     /translation="MLINRLAILLAERSMSGARLASDTGIAQSTISKITSNKSKQVDY
                     ETVNKICNTLGVTSDDFFDYSPIDYEIKYFIDEDSGDIFTFIKILDRDRPVATLEYKV
                     DLEFHINTNKPDNLVGIEEIKRNYQQVEIITVKVCLKKQNKETYDKFTKLPSNFQNIF
                     TNDLLQVVGKNI"
     gene            677..880
                     /locus_tag="VW854_000451"
     CDS             677..880
                     /locus_tag="VW854_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000916878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0261741.1"
                     /translation="MLKNNLLIVFAEKKTNASKVSNETGIAESTISNLTNNKTDVKLS
                     TLIKLCNALKVRLSDLIEFSPEN"
     gene            895..1524
                     /locus_tag="VW854_000452"
     CDS             895..1524
                     /locus_tag="VW854_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HEV0261742.1"
                     /translation="MELVYMDGKKEPYTTSEIIAECAGVTHHTIQELLRKHKADFESY
                     GIIAFEMRKLDGRGRPMKIYRLNEQQATLLITYLKNTEPVRRFKMNLVKAFFEMREEL
                     SKFRMQRALEKPKRKTLHDSIETWEAKPKHAHSTMNNLLLKAVTDRNAKQLREERGGY
                     NGIDSLTSDELEQYQAFEDMVIAMIGLNMSYQEIKAMAFRNKKTRQRSA"
     gene            1959..2411
                     /locus_tag="VW854_000453"
     CDS             1959..2411
                     /locus_tag="VW854_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261743.1"
                     /translation="MEETAYENLTRRIDRISAEMREIGEKTGLSRLSLLANQTKFIKE
                     NLHRLLWIELPELNDSYKIEAVSKSTTGMFFHPGIFEMDAMRQAFFKRQAMHFFENEA
                     EQQAYIEHAEKEYLEATITLKDILFNSKNGTQRVNKSCLIEKFEEAMQ"
     gene            2408..2608
                     /locus_tag="VW854_000454"
     CDS             2408..2608
                     /locus_tag="VW854_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261744.1"
                     /translation="MTLDLDNMTQAEFDKQMAEIKERNPNLFQFIADFVDRKVTPKEV
                     DEFLKMGRSDQVDYIKNYKARA"
     gene            2610..2825
                     /locus_tag="VW854_000455"
     CDS             2610..2825
                     /locus_tag="VW854_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261745.1"
                     /translation="MNELDLTNTQAVISMVVLIGLLLYLNHRDRKKSAQFERENQQTI
                     ETPSEDLSPDYGRYIQLAGVRVYGGMK"
     gene            2822..3115
                     /locus_tag="VW854_000456"
     CDS             2822..3115
                     /locus_tag="VW854_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261746.1"
                     /translation="MSYTMNLYPDIEKISDETYFTREIFLATCLNNTIRIAVARGVLT
                     SQLLDKETKEVVCKTLDTIIENQKAILQGVASGQITFLNKSKEEEELNESIRE"
     gene            3096..3437
                     /locus_tag="VW854_000457"
     CDS             3096..3437
                     /locus_tag="VW854_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV0261747.1"
                     /translation="MSLSENDKRVLRLIKVGAENSITGAEISLITKLAERTVQDIISR
                     LIMRYGIPIIGVRHGTFRGYFIPANKEELLDGAKTFYNQIREEEKRLTVLLNADLESY
                     KQLLKGADEYV"
     gene            3430..3711
                     /locus_tag="VW854_000458"
     CDS             3430..3711
                     /locus_tag="VW854_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEV0261748.1"
                     /translation="MFSLSQESEQDLAHGILEVVERYLEARDTVKPRLTGLISAQEVM
                     GELDIKYMTLQKWEKAGLRRYQPPLEDTRKIYYKISDIWKFLGVENGKV"
     gene            3846..4706
                     /locus_tag="VW854_000459"
     CDS             3846..4706
                     /locus_tag="VW854_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="HEV0261749.1"
                     /translation="MAIYEAKGFQSNLVYPFDKIEPFQYIELFKPLVVPEGANIEEFK
                     RTQAPYCISGKVTPEKNGSYKRNNSSLIYRDLIFLDYDDIQGTTEDFIEAVSSTLFGY
                     SYILYPTIKHSIEKPRFRLVVKSNNVMNEATYKQVVKEIADKIGLPFDMASLTWSQLQ
                     GLPVTTGDPATYQKIVEHGLDYPVPKVEPRAKQGTTERYKPRVSGQRSMTMRIIDTLF
                     NGFGDEGGRNVALTRFVGLLFNKWVDCDLETAYELTKIANSVTVEPLPIEELDRTFSS
                     IARAEYRKRG"
     gene            4750..6228
                     /locus_tag="VW854_000460"
     CDS             4750..6228
                     /locus_tag="VW854_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase family protein"
                     /protein_id="HEV0261750.1"
                     /translation="MEVREQEHPPRTMKELENRIFKAGEEWRAEHTETKVNETTGDVT
                     EKVAIPQTFTVAKILSEIVTFTFISKSNIADYSLLYIYDLDEGIYTASNDLFNLLCKT
                     FDVRIKPREWPQIKLMVRTLTKIRKPLESSNLIPVQNGIINLETKELFPFSPKYVITS
                     KISTAYHAPKRVPTDREGKTFDDWLNSIACNDSELVTLFWQIILEAINSNHTRNKFAI
                     FYGDGNNGKGTFQRFLINLIGESNISALKPAQFAEKHNLETLVGKVCNIGDEAPNEYL
                     KNPSDLMSITSGDTVLVNPKGRPAFEATFKLFNIFSGNYIPNGGNKTKGWYRRIMIVP
                     FNADFNGEKEKPWIKNEFLANDEVLEYALYKAINQEPFTHFIEPKAVKGLLEEYQEEN
                     DYLLSWVKHEYMERGWHELDVVPVFILTRSLKHYAEDMGIPKPNVYGAGKETIKHLQQ
                     LTPNRYSLRKKRVPSEDFEKLEAWDVDKIKMRTPQHSITKQE"
     gene            6579..6746
                     /locus_tag="VW854_000461"
     CDS             6579..6746
                     /locus_tag="VW854_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261751.1"
                     /translation="MKVKLFEYTRRWEKEDFEKEINDFMATVEVLDVKFSGTPSSESF
                     NSVAIVLVLYR"
     gene            6829..7359
                     /locus_tag="VW854_000462"
     CDS             6829..7359
                     /locus_tag="VW854_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261752.1"
                     /translation="MTLKTFSDKAKTFTFTYEFKDLDTAMVAGHALLGYMTGTYEVPS
                     ISITHKDKGTLVAEYVEDHKLNKTFKRICDSFKDYYNQPVDDEAFEERYKRERVLQLK
                     ESEDFENLLEKVTDYELELLDYADRLLSDKPIPMDSMTAFGTLEMLGDESISLLQKLD
                     VEGEYKGLAGYTEHLK"
     gene            7430..7933
                     /locus_tag="VW854_000463"
     CDS             7430..7933
                     /locus_tag="VW854_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261753.1"
                     /translation="MKRLAIETITKPMKLRGISKGIAELDGQRLEIDLDNLMIDFGGE
                     SFELDRIAGTKGGNRYFFLCPDCGRRCRLLYKRYLYFSCGTCLDIHKSTLNRSKTNCQ
                     YYWELALKEARKVEPGWSPRRGGYMFDSFPERPKYMKRDRYHKHYKKFLKYTKKGDRF
                     WLNGLRL"
     gene            9531..9887
                     /locus_tag="VW854_000464"
     CDS             9531..9887
                     /locus_tag="VW854_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEV0261754.1"
                     /translation="MQLKYQSLESGLLAGSSQFQTRVSTSKTNTAENKLLDTLELRDK
                     IAEQIQAIINERMEAMDMINQLEEIEEWLVLVMIYFNNLPMVKICNDLNFSKVQIYRI
                     RKKAIENLAKVKNANR"
     gene            9874..10212
                     /locus_tag="VW854_000465"
     CDS             9874..10212
                     /locus_tag="VW854_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261755.1"
                     /translation="MPIDKEETIKQAYKFLKSYHSLVKLSLGGQDGAFEAKAMELLRV
                     IEAFRDNLDDVRHEIFANLFTRRTGERLKLWQIYEALDIDKAEYERLKAEILLDFTKS
                     YCGGVLLKKC"
     gene            10866..13697
                     /gene="uvrA"
                     /locus_tag="VW854_000466"
     CDS             10866..13697
                     /gene="uvrA"
                     /locus_tag="VW854_000466"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEV0261756.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAIKASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKIQKDGYVRVRVDGEVYDVTEVLELSKSRQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLHEGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVIGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            13690..14751
                     /locus_tag="VW854_000467"
     CDS             13690..14751
                     /locus_tag="VW854_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV0261757.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLDLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASSLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            14883..15281
                     /gene="spx"
                     /locus_tag="VW854_000468"
     CDS             14883..15281
                     /gene="spx"
                     /locus_tag="VW854_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEV0261758.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            15347..15916
                     /locus_tag="VW854_000469"
     CDS             15347..15916
                     /locus_tag="VW854_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV0261759.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGFENLLKNEPSKYISDRLANGATEQ"
     gene            16003..16269
                     /locus_tag="VW854_000470"
     CDS             16003..16269
                     /locus_tag="VW854_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEV0261760.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            16273..16692
                     /gene="ruvX"
                     /locus_tag="VW854_000471"
     CDS             16273..16692
                     /gene="ruvX"
                     /locus_tag="VW854_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEV0261761.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            16708..17013
                     /locus_tag="VW854_000472"
     CDS             16708..17013
                     /locus_tag="VW854_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEV0261762.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            17221..18471
                     /locus_tag="VW854_000473"
     CDS             17221..18471
                     /locus_tag="VW854_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV0261763.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLTDQVKE
                     MEKTLLQTPAQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLACQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNLSQLNIGLEGVYQQENAALALQTFLLFMRERKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLAEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTEKLPQVELKVTGFDYQGALDERDVTGYDIVSSYREFISDFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            18468..19010
                     /locus_tag="VW854_000474"
     CDS             18468..19010
                     /locus_tag="VW854_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEV0261764.1"
                     /translation="MTPFFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQVQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDIDG
                     TYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTLDSANQRMIIGDDVKIYTVNG
                     NQIVVDDMDRDPSDQIVLTK"
     gene            19156..20688
                     /gene="cls"
                     /locus_tag="VW854_000475"
     CDS             19156..20688
                     /gene="cls"
                     /locus_tag="VW854_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEV0261765.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            20766..20909
                     /locus_tag="VW854_000476"
     CDS             20766..20909
                     /locus_tag="VW854_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261766.1"
                     /translation="MLTKEEVNDMIEFKQTHLHRFREIETFVKLCKKSLKQPSQADNP
                     RTF"
     gene            20946..22484
                     /locus_tag="VW854_000477"
     CDS             20946..22484
                     /locus_tag="VW854_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEV0261767.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSNYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVEVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            22604..24811
                     /gene="nrdD"
                     /locus_tag="VW854_000478"
     CDS             22604..24811
                     /gene="nrdD"
                     /locus_tag="VW854_000478"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEV0261768.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSNQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            24823..24963
                     /locus_tag="VW854_000479"
     CDS             24823..24963
                     /locus_tag="VW854_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261769.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            25007..25513
                     /locus_tag="VW854_000480"
     CDS             25007..25513
                     /locus_tag="VW854_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0261770.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKFQSPHDGGFWDTENFVYEDWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAAILANGGIFEDARNGVER
                     YWIEVANE"
     gene            25506..26096
                     /gene="nrdG"
                     /locus_tag="VW854_000481"
     CDS             25506..26096
                     /gene="nrdG"
                     /locus_tag="VW854_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEV0261771.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            26093..26709
                     /locus_tag="VW854_000482"
                     /pseudo
     CDS             26093..26709
                     /locus_tag="VW854_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
     gene            26970..27278
                     /gene="rpsJ"
                     /locus_tag="VW854_000483"
     CDS             26970..27278
                     /gene="rpsJ"
                     /locus_tag="VW854_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEV0261772.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            27494..28120
                     /gene="rplC"
                     /locus_tag="VW854_000484"
     CDS             27494..28120
                     /gene="rplC"
                     /locus_tag="VW854_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEV0261773.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            28145..28768
                     /gene="rplD"
                     /locus_tag="VW854_000485"
     CDS             28145..28768
                     /gene="rplD"
                     /locus_tag="VW854_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEV0261774.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            28768..29064
                     /locus_tag="VW854_000486"
     CDS             28768..29064
                     /locus_tag="VW854_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEV0261775.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            29082..29915
                     /gene="rplB"
                     /locus_tag="VW854_000487"
     CDS             29082..29915
                     /gene="rplB"
                     /locus_tag="VW854_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEV0261776.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            30019..30300
                     /gene="rpsS"
                     /locus_tag="VW854_000488"
     CDS             30019..30300
                     /gene="rpsS"
                     /locus_tag="VW854_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEV0261777.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            30312..30656
                     /gene="rplV"
                     /locus_tag="VW854_000489"
     CDS             30312..30656
                     /gene="rplV"
                     /locus_tag="VW854_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEV0261778.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            30669..31322
                     /gene="rpsC"
                     /locus_tag="VW854_000490"
     CDS             30669..31322
                     /gene="rpsC"
                     /locus_tag="VW854_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEV0261779.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            31326..31739
                     /gene="rplP"
                     /locus_tag="VW854_000491"
     CDS             31326..31739
                     /gene="rplP"
                     /locus_tag="VW854_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEV0261780.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            31749..31955
                     /locus_tag="VW854_000492"
     CDS             31749..31955
                     /locus_tag="VW854_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEV0261781.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            31980..32240
                     /gene="rpsQ"
                     /locus_tag="VW854_000493"
     CDS             31980..32240
                     /gene="rpsQ"
                     /locus_tag="VW854_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEV0261782.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            32266..32634
                     /gene="rplN"
                     /locus_tag="VW854_000494"
     CDS             32266..32634
                     /gene="rplN"
                     /locus_tag="VW854_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEV0261783.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            32712..33017
                     /gene="rplX"
                     /locus_tag="VW854_000495"
     CDS             32712..33017
                     /gene="rplX"
                     /locus_tag="VW854_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEV0261784.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            33041..33583
                     /gene="rplE"
                     /locus_tag="VW854_000496"
     CDS             33041..33583
                     /gene="rplE"
                     /locus_tag="VW854_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEV0261785.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            33601..33870
                     /gene="rpsN"
                     /locus_tag="VW854_000497"
     CDS             33601..33870
                     /gene="rpsN"
                     /locus_tag="VW854_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEV0261786.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            34155..34553
                     /gene="rpsH"
                     /locus_tag="VW854_000498"
     CDS             34155..34553
                     /gene="rpsH"
                     /locus_tag="VW854_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEV0261787.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            34745..35281
                     /gene="rplF"
                     /locus_tag="VW854_000499"
     CDS             34745..35281
                     /gene="rplF"
                     /locus_tag="VW854_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEV0261788.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            35365..35721
                     /gene="rplR"
                     /locus_tag="VW854_000500"
     CDS             35365..35721
                     /gene="rplR"
                     /locus_tag="VW854_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEV0261789.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            35739..36233
                     /gene="rpsE"
                     /locus_tag="VW854_000501"
     CDS             35739..36233
                     /gene="rpsE"
                     /locus_tag="VW854_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEV0261790.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            36205..36429
                     /gene="rpmD"
                     /locus_tag="VW854_000502"
     CDS             36205..36429
                     /gene="rpmD"
                     /locus_tag="VW854_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEV0261791.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            36574..37014
                     /gene="rplO"
                     /locus_tag="VW854_000503"
     CDS             36574..37014
                     /gene="rplO"
                     /locus_tag="VW854_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEV0261792.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            37027..38337
                     /gene="secY"
                     /locus_tag="VW854_000504"
     CDS             37027..38337
                     /gene="secY"
                     /locus_tag="VW854_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEV0261793.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            38488..39126
                     /locus_tag="VW854_000505"
     CDS             38488..39126
                     /locus_tag="VW854_000505"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEV0261794.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGVINIEVNSDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            39243..39461
                     /gene="infA"
                     /locus_tag="VW854_000506"
     CDS             39243..39461
                     /gene="infA"
                     /locus_tag="VW854_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEV0261795.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            39486..39602
                     /gene="rpmJ"
                     /locus_tag="VW854_000507"
     CDS             39486..39602
                     /gene="rpmJ"
                     /locus_tag="VW854_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEV0261796.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            39620..39985
                     /gene="rpsM"
                     /locus_tag="VW854_000508"
     CDS             39620..39985
                     /gene="rpsM"
                     /locus_tag="VW854_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEV0261797.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            40003..40386
                     /gene="rpsK"
                     /locus_tag="VW854_000509"
     CDS             40003..40386
                     /gene="rpsK"
                     /locus_tag="VW854_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEV0261798.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            40429..41364
                     /locus_tag="VW854_000510"
     CDS             40429..41364
                     /locus_tag="VW854_000510"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEV0261799.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            41376..41762
                     /gene="rplQ"
                     /locus_tag="VW854_000511"
     CDS             41376..41762
                     /gene="rplQ"
                     /locus_tag="VW854_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEV0261800.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            42027..42293
                     /locus_tag="VW854_000512"
     CDS             42027..42293
                     /locus_tag="VW854_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEV0261801.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            42303..43640
                     /locus_tag="VW854_000513"
     CDS             42303..43640
                     /locus_tag="VW854_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEV0261802.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGKEGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
     gene            43884..44576
                     /locus_tag="VW854_000514"
     CDS             43884..44576
                     /locus_tag="VW854_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV0261803.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEIIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(44679..46324)
                     /locus_tag="VW854_000515"
                     /pseudo
     CDS             complement(44679..46324)
                     /locus_tag="VW854_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(46314..47405)
                     /locus_tag="VW854_000516"
     CDS             complement(46314..47405)
                     /locus_tag="VW854_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0261804.1"
                     /translation="MSEIEIINAKKIYHDVSVIENLNITIPKGSLFTLLGASGCGKTT
                     LLRMIAGFNSIKDGEFYFDDTKINNMEPSKRNIGMVFQNYAIFPHLTVRDNVAFGLMQ
                     KKVPKEELIQQTNKYLELMQIAQYADRKPDKLSGGQQQRVTLACALAVNLSVLLMDEP
                     LSNLEAKLRLDMRQAIREIQHEVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRP
                     KELYHKPANEFVATFIGRTNIIPANLEKRSDGAYIVFSDGYALRMLALDQVEEQAIHV
                     SIRPEEFIKDESGDIEGTISDSVYLGLNTEYFIETGFASKIQVSEESTFEEDLQKGNR
                     IRLRINTQKLNIFSADGSQNLIKGVNHGT"
     gene            complement(47418..48539)
                     /locus_tag="VW854_000517"
                     /pseudo
     CDS             complement(47418..48539)
                     /locus_tag="VW854_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931989.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            complement(48756..48953)
                     /locus_tag="VW854_000518"
     CDS             complement(48756..48953)
                     /locus_tag="VW854_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV0261805.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSPLSAISDQVGFSDYKYFAK
                     VFKKYLHISPKKLKSLGRIVK"
     gene            complement(49028..49804)
                     /locus_tag="VW854_000519"
     CDS             complement(49028..49804)
                     /locus_tag="VW854_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEV0261806.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGVNALHPEDLIDYQKVFLNHHINCYF"
     gene            49926..50672
                     /locus_tag="VW854_000520"
     CDS             49926..50672
                     /locus_tag="VW854_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV0261807.1"
                     /translation="MKRLEQIIKLVSEHEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     HQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            50685..>50783
                     /locus_tag="VW854_000521"
     CDS             50685..>50783
                     /locus_tag="VW854_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEV0261808.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIY"
BASE COUNT        16160 a         9209 c        11097 g        14317 t
ORIGIN      
        1 tatattttta ccaactactt gaagtaaatc gtttgtaaaa atattttgga aattgctagg
       61 taatttagtg aatttatcat aagtttcttt gttttgtttc ttcaaacaaa ctttcactgt
      121 gataatttct acttgctgat agttacgctt aatttcctca ataccaacaa gattatctgg
      181 cttattagtg tttatatgaa attcaaggtc cactttatac tctagtgtag caaccggtct
      241 atctctatct aagattttaa taaaagtaaa tatatctcca ctatcttcgt cgataaaata
      301 ttttatctca taatctattg gagaataatc aaaaaaatcg tccgaagtaa cccctaaagt
      361 gttacagatt ttattaacag tttcataatc aacttgtttt gatttatttg aagtaatctt
      421 agaaattgtt gattgtgcaa ttcccgtatc acttgctaaa cgcgcaccac tcattgacct
      481 ttcagccaat agtatagcca atctatttat taacataata ccacctcttt ttatttatta
      541 tagcgcacac gctaataaaa gtaaaatata aaataataat ttaagtgttg acaatatgtt
      601 ttatatgttg tatcatatca ttaaattagt cattgcgcta taatgattag ttaataaaaa
      661 ataataagga gcgcctatgc taaaaaataa cttactaatt gtttttgctg aaaaaaagac
      721 taatgctagt aaagtttcca acgaaacagg tatagctgag tctacaattt caaacttaac
      781 aaacaacaaa actgacgtaa agctttctac ccttatcaag ctttgtaacg ctttaaaagt
      841 tcgtttgtct gatttaattg aattttcacc agaaaattag aaaggagcaa accaatggaa
      901 ctagtctaca tggacggaaa gaaagagccg tatactacga gtgagatcat cgctgaatgt
      961 gctggagtca ctcatcacac aatacaagaa cttttaagga agcataaagc tgattttgaa
     1021 agctacggaa ttatcgcatt tgaaatgcgt aaattagacg gccgagggag accaatgaag
     1081 atatatcgcc taaatgaaca acaagcgacc ttgctgatca cttatttaaa gaatactgaa
     1141 cctgtacggc ggttcaaaat gaacctagtc aaagcattct ttgaaatgcg tgaggagctt
     1201 tctaagttcc gtatgcagag ggcgctagaa aagccaaaaa gaaaaacctt gcatgacagt
     1261 atagagacat gggaggcaaa acccaagcac gcgcatagca ccatgaacaa cctgctacta
     1321 aaagcagtaa ctgacaggaa cgctaaacag ttaagggaag aacgtggggg ctacaatggt
     1381 attgatagct tgaccagtga cgagctggag caataccagg catttgagga tatggtaata
     1441 gccatgatcg gcttgaatat gagttaccaa gaaatcaagg ctatggcatt cagaaataaa
     1501 aaaacacgcc aaagaagcgc gtgattgcaa caaaaaaggc ttaccgagac caatcagcaa
     1561 agcctttcac actatgacta aaacgaattt aacaaaaagc aggcaagcta ttatcaaaga
     1621 ggttttagta aagatttata cctagattat agcatattta gggcaatttg accatacgga
     1681 gagcgccaac tctttaaact ggtactttct cacgctttca atttggcgag tctgagcgtg
     1741 aggaatagcg ttataagaaa caactcaaaa agccccacgt tcagaaattt ggcgaccgag
     1801 agcatgaggt tgtagacaag aaaaactgta taagaaacaa ccattcaaaa tccccacact
     1861 cgccatcgct caactttgag tgtgaggata tcctggatag taaaaggcat taaaaagccc
     1921 ttagggcaac tacacaacat agaaacagag gtaaaaatat ggaagaaaca gcatacgaga
     1981 atttaactag acgtatagac agaattagcg cagaaatgcg agaaattggc gaaaaaactg
     2041 ggcttagtag actttcgtta ttggcaaatc agacaaaatt catcaaagaa aatttacacc
     2101 gtttactttg gattgaactt cctgaattga atgacagcta taaaatcgag gcagtttcta
     2161 aaagcactac gggaatgttt ttccaccctg gtatttttga aatggacgct atgcgacaag
     2221 cattctttaa acgccaagct atgcactttt ttgagaacga agcagagcaa caggcgtata
     2281 tagaacatgc tgaaaaggag tatttagagg ctactataac cttaaaagat attcttttta
     2341 actctaaaaa tggaactcag agagtaaata aaagttgtct tatagagaag tttgaggagg
     2401 caatgcaatg acactagacc tagacaacat gacacaagca gaatttgata aacaaatggc
     2461 tgaaatcaag gagagaaatc cgaatctctt ccagtttatt gctgattttg tagatagaaa
     2521 ggtaacaccc aaagaggtag atgagttttt gaagatggga cgaagcgacc aagtggacta
     2581 catcaagaac tataaagcga gggcatgaca tgaatgaatt agatttgacc aatacacagg
     2641 cggtaatctc tatggtggta ctgattggct tactgcttta tttaaaccac cgagaccgta
     2701 aaaaaagcgc ccaatttgag cgagaaaacc aacagacgat agaaacacct agcgaggatt
     2761 taagccctga ttatgggcgt tatatccagc ttgcaggggt aagagtttac ggagggatga
     2821 aatgagttac acaatgaatt tataccccga tattgaaaaa atatcagacg aaacctattt
     2881 taccagagag atttttctag caacgtgctt gaataacaca atcagaatag cagtagccag
     2941 aggagtgcta acatctcaac ttttagacaa agaaactaaa gaagttgttt gtaaaacctt
     3001 ggatacaatc atagaaaatc aaaaagctat tttgcaaggg gtagcaagcg gacaaataac
     3061 ctttttgaat aaaagcaaag aagaggagga actgaatgag tctatcagag aatgacaaac
     3121 gagtattaag actaatcaag gtaggtgctg agaactccat aacaggggca gaaatcagcc
     3181 tgatcactaa gttagcagaa agaaccgtgc aagatattat cagccgtctg atcatgcgct
     3241 acggcattcc tatcatcggg gtacgtcatg gaacatttag aggatatttt atcccagcta
     3301 acaaagagga gttactggac ggtgcaaaaa cattttacaa tcagatacga gaagaagaaa
     3361 agcgcctaac tgtcctttta aatgctgatt tagagagtta taagcaacta ctgaaggggg
     3421 cggacgaata tgtttagcct aagccaagaa agcgagcaag acctagcgca tggcattctg
     3481 gaggtagtgg aaagatacct ggaagcgcgt gatacagtca aaccacgact gacaggttta
     3541 atatcagccc aggaagtcat gggcgagtta gatataaaat acatgacact ccaaaaatgg
     3601 gaaaaagcag ggctaagacg gtatcagcca ccgctagaag atactaggaa aatctattat
     3661 aaaatatctg acatctggaa gtttttgggg gtggaaaatg gcaaggtata gtattcatcc
     3721 ggcagataat aaagggcatt atcacaatat taaattttac aaagagcgca ggccaaccct
     3781 agaccagcta aaggaacagc aaaggcttaa aaagctgaag aaaaaacgta gaaaggggcg
     3841 ataatgtggc aatttatgag gctaaaggct ttcagagtaa tcttgtctat ccatttgata
     3901 aaattgagcc gttccagtac attgaactct ttaagccttt ggtagtccct gaaggggcaa
     3961 acattgaaga atttaaacgt acacaagcgc cctactgtat cagtgggaaa gtgacaccag
     4021 aaaagaatgg cagttacaag cgaaacaact ccagtctaat ctatcgggat ttgattttcc
     4081 ttgattatga cgatatacag gggacgactg aggacttcat agaagccgtt tctagcactt
     4141 tgtttggcta ctcttatatc ttatacccga ctatcaaaca tagcatagaa aagccccgtt
     4201 ttcgccttgt ggtgaaatct aacaacgtga tgaacgaggc aacctataag caggtagtaa
     4261 aagagattgc tgacaagatt gggctaccct ttgacatggc cagtttaacc tggtcacaac
     4321 tccaagggct accagtaaca acaggcgacc cagcaactta tcaaaagatt gtagagcatg
     4381 gacttgatta tccagtcccc aaggttgaac caagagcgaa gcaaggaact actgagcgtt
     4441 ataaacctag agtaagtggc cagaggtcaa tgactatgag gattattgac acgcttttca
     4501 acggatttgg agacgaagga gggcgcaacg tagcgctgac acgctttgtg ggattactct
     4561 tcaataagtg ggttgattgt gacctagaga ccgcctatga actgacaaag atagcaaata
     4621 gcgtgacagt tgaaccattg cctattgaag agctagaccg cacttttagc agtatagcac
     4681 gggctgagta tagaaagaga ggttagaacc atagaactag aagaattagc aaacctaaaa
     4741 agtgaaatca tggaggtcag agagcaagaa caccctccta gaacgatgaa ggagcttgaa
     4801 aatcgtatct ttaaagctgg tgaagaatgg cgggcagaac ataccgaaac taaggtaaat
     4861 gagaccacag gggacgttac tgaaaaggtt gccatacctc agacattcac tgtagctaaa
     4921 atactgagcg agattgtcac gtttactttt atcagcaaga gcaatatagc tgattatagt
     4981 ctactctata tctacgattt agacgagggt atctatacag ctagtaatga tctatttaat
     5041 ctattgtgta agacttttga tgtgagaatt aaacccagag agtggccgca gattaagtta
     5101 atggttagaa cactaacaaa gatacgaaaa cctttagaaa gttccaacct cattcccgta
     5161 cagaacggaa ttatcaactt agaaactaag gagctattcc cctttagtcc taagtatgta
     5221 attacaagca agataagcac ggcataccac gcgcctaaac gagtcccaac agatagagag
     5281 ggaaaaactt ttgatgattg gctaaactca atcgcttgta atgatagtga gctagtgaca
     5341 ctgttttggc aaattatcct tgaagctatc aactctaacc atacccgaaa taagtttgct
     5401 atcttctacg gggacggtaa caatggtaaa gggacatttc agcgctttct tatcaatctg
     5461 ataggggaaa gcaatatatc agccttaaaa cctgcacagt ttgctgaaaa gcataactta
     5521 gaaaccctgg taggtaaagt ttgtaatatc ggagatgaag ccccaaatga atacttgaaa
     5581 aatccgtctg atttaatgag tatcactagt ggcgatacag tactggtcaa ccccaaagga
     5641 cgcccagcct ttgaggctac tttcaagtta ttcaatatct tttcagggaa ttatatcccc
     5701 aatggtggca acaagacaaa aggctggtat cgtagaatta tgattgtccc ttttaatgct
     5761 gactttaacg gggagaagga aaagccttgg attaaaaatg aatttttggc caatgatgaa
     5821 gtattagagt acgcacttta caaagctatc aatcaagagc cttttacaca ctttatcgaa
     5881 cccaaagcag tcaaaggcct tctagaagaa taccaggaag aaaatgacta cttgctttca
     5941 tgggttaaac atgaatacat ggaaagaggt tggcatgagc tggacgtggt acccgttttt
     6001 atactgacca gatcactaaa gcactatgcc gaagatatgg gaatacccaa acccaatgtt
     6061 tacggggctg gtaaagaaac tatcaagcac ttacaacagt tgacacctaa tcggtattct
     6121 ctaagaaaga aaagagttcc ttcagaagat tttgaaaaac tagaggcatg ggacgttgac
     6181 aagataaaaa tgcggacacc tcaacactct atcactaaac aagaataagt gtgacgttct
     6241 ttttttgctt aaacccttga taatactgat tttcttaaaa aaagtgtgac gttgtgtgac
     6301 gttctcaaaa aagaaggtca caccccttga agccttgata tatctagctt taggggaata
     6361 ggtgtgacgt tgtgacattc tttccaactc ttatatatag agaaaaatgc agtatttata
     6421 tataggaacg ataaaacaaa agaacgtcac agggtcacag gggtggtgaa acacctagag
     6481 cctcaagggt ttgaacgtgt gacgttcttt gtcacagaag gtcacagaac acgaaaaata
     6541 ttacataatt taccttaaaa taaagaaatg acaacaacat gaaagttaaa ctatttgaat
     6601 atactagacg atgggaaaaa gaagactttg agaaagagat caatgacttc atggcaactg
     6661 ttgaagtgct ggacgttaaa ttttctggta caccatctag tgaatccttt aattcggtgg
     6721 ctatcgtatt ggtattatac agataacaaa atggggtaga tagtaccgac ccccttgtaa
     6781 tggtgtaact actagtgaca cccttaaaac agaaagagga aaaacaacat gacattaaaa
     6841 acattttcgg ataaagccaa aacatttact ttcacttacg aatttaaaga cctagatact
     6901 gctatggtag caggtcacgc gctactggga tatatgactg ggacttatga agtaccatct
     6961 atttcaataa ctcacaagga taaagggacg cttgtcgctg agtatgtgga agatcacaag
     7021 ctaaacaaga ctttcaagcg tatttgtgac agtttcaaag actattacaa ccaaccagtg
     7081 gacgatgaag catttgaaga acgttataaa cgggagcgcg tgctccaact caaagagtca
     7141 gaggactttg agaacttgct ggaaaaagta acagactacg agctggaact attagactat
     7201 gctgatcgct tgctttctga taagcctatc cctatggact ctatgaccgc ttttggtacg
     7261 ttggaaatgc tgggcgatga aagtattagc ctactccaaa agttggacgt agagggcgaa
     7321 tataaaggcc ttgctggtta tacagagcat ttgaagtaga acacagggga gcaatcccct
     7381 ttttgttatc aagtcaatag ttttgggttt ttccatatag ggggaaataa tgaagcggtt
     7441 agcaatagaa acaatcacta aaccaatgaa gctgagagga atatctaagg gtatagctga
     7501 gttggacggt caacgtttag agattgattt ggataattta atgattgatt ttggtggaga
     7561 gtcatttgaa ttagacagaa tagcaggaac taagggaggt aatagatatt tctttctttg
     7621 ccctgattgt gggagacggt gcaggttgct ttataaacgg tatttatatt ttagttgcgg
     7681 tacttgtcta gatatccaca agagcaccct gaaccgtagt aaaacgaatt gccaatacta
     7741 ctgggagctt gcactaaaag aagccagaaa ggtagaacca gggtggagtc ctagacgtgg
     7801 tggatatatg tttgatagtt tccctgaaag accaaagtat atgaagcgcg acaggtatca
     7861 caagcactat aagaagtttt tgaagtatac taaaaaagga gatagatttt ggctgaatgg
     7921 tttgagattg taggacaata tgaagtagtg tgcaatttac aaaacaaaca aatactacat
     7981 attagacggt ttgaatgtta aaaaagctag atatgtcaag gatttaagta agtagagcgt
     8041 tccaaaaaat ggtgcggtaa gcgttccgaa aaatggtaag gttttggtaa ggttgcatat
     8101 ataaaaagta taagattagt ataaggttgt gtattttaaa atactaaggt tttacaaagg
     8161 tacagggctt tttttctagt gatataacca caaggatacc acaaggtaga gagttttacc
     8221 gagggttaga aaaggtgaga ttttgcttag gtagtggcag taaatatgcc tgataaatgg
     8281 aataaagata gttgtaagat tagagtaaga tgttattagt aatttgatta gtagatacat
     8341 ttacctttct ctaagtgtta tgatcaaatt gaattagtca gtaatcaaca atactttgtg
     8401 ttgattgcat tctttattga ttgatataat caaatcgata acaatagatg aatgaaatgc
     8461 aatgttagca atagttaaca aatgatttct ctttctcttt tatgtttgaa tgacatgaga
     8521 ttgaactact ggaaaacgat aaggggataa aaaagtatgc aaataccccc cttatttttg
     8581 aacgggggta tataatgttc ggatatagga acgcgccccc cttctgtgca aaaatttccc
     8641 tttttgaaat gtttttaatt tttgtagaat gcagtattac taagtgttta gcttgatttt
     8701 ttgaccgata aagctactcc aattttataa cacagtgggg ctatcttcct ccccacgcgc
     8761 ttctatgaac ttcacactgt cattgtacat tttttctcac gggaaagcat tgaactccga
     8821 gcgaacgccg agaaagtgga tatatcctcc tatcatttat caagcagata agggcgacaa
     8881 taagccttct taattgctta caaaacgata gggagttaag acgaatttaa aagctcctta
     8941 ttttcgtttt tctccttgtc aaatagtaag gaaataagac aggaaataac atcgcttatt
     9001 ttgattttgc tccctgaaaa actgtaagga gataaggaga cttattagct tccttaattt
     9061 ggattttctc cttacaaaat gacagggaaa taagaactaa aataactccc cttatttttc
     9121 tacaaaatga taagcagata agccaataaa taacttgcct taattgcttt caaacatgaa
     9181 caagtatgaa catattccac cagtcgttga aaatacaaaa agccaaggct taccacctca
     9241 gaatgattag gttttggtta ggttttacat ttaaaaatag caaggtacag gactttattc
     9301 tagagataaa atactaagat ttcctaagat acaaatactt ttagagataa agaacgttaa
     9361 ggagggtata atcgaactcc tagaaaacgc aaaaaaagcc agcacagggc tgacttcttt
     9421 ataaattatt ccttaaaatt attataccat aaaggagtag caacttgact attaaagaaa
     9481 tagaggaata actaaaaaga gtaaagaaag gaaatgccct tatccaggaa ttgcaactta
     9541 aataccagag tttagaaagt ggactgcttg caggatctag tcagtttcaa acacgggtaa
     9601 gcacaagcaa aaccaatacc gctgaaaata aactgcttga tactttggaa ttaagagata
     9661 agatagcgga gcagatacaa gctattatta atgaacgtat ggaagcaatg gatatgataa
     9721 accagttaga agaaatcgaa gaatggttag tgttggtaat gatatacttt aataatttac
     9781 ctatggttaa aatctgcaat gatttaaatt ttagcaaggt acagatttac agaattagga
     9841 aaaaagccat tgagaacttg gccaaggtga aaaatgccaa tcgataaaga agagacgata
     9901 aaacaagcat acaaatttct aaaaagctat cactcgttgg ttaaactgag cctaggcggt
     9961 caagatggag catttgaagc caaagcgatg gagctactgc gcgtgatcga agccttcagg
    10021 gacaatctgg acgatgtacg acatgagata ttcgccaatc tctttacaag gcgaactggg
    10081 gagaggttaa agctatggca gatatacgag gcactagaca ttgataaggc tgagtatgag
    10141 cgtctcaagg ctgagatact gctagacttt actaaaagct attgtggtgg tgttttgttg
    10201 aaaaaatgtt gagatttaag cgagagaaca tggacattta tggacacata tcgccagttg
    10261 ttgaacagct cataaaatgt tgtcaggtac taacaagttc ttacacatcc cgcccgtcgt
    10321 cgaaaaacta aaaaataatc tctgggtctg aaaagttcct acatattcca ccagaacgga
    10381 gagttagaag cgtaaatgtt ggcaaatgta ggctgaggct tcttattcgc cccctatttt
    10441 gctttgtttc gtactggaca actaaaacac catggataaa ctacaagccc tcagaaacga
    10501 atctgggggc ttttgtgagg tcataatctt ataaaccacg cgcttcatgg tataatatac
    10561 ctatcagcaa ccagcaataa aaaaagcacg tttgaccgtg ctagtttctt gcctgctgaa
    10621 ctcgtcaaat cttagccctt ttgtggggct ttttttgctc ccctttttgc gtactttggg
    10681 ggagcaagta gtaaaaagta atgttagtaa tttttattaa aaagcgcgtg atataagggt
    10741 taggaagtcc taaggaaaca taaaataaga agttttttat tatactacaa aatcggcctt
    10801 ttgggtaata gaatctcact ttttttggta taatggtaag caataatgga ctagaaagaa
    10861 caaagatgca agataaaatt gtcattcatg gggcgcgtgc ccataattta aaaaatattg
    10921 atgtggagat tccgcgagac aagttggttg tcgtgactgg cttgtcaggt tcagggaagt
    10981 ccagtctggc ttttgatacc ctctatgcgg agggacaacg tcgctatgta gagagtttgt
    11041 ccgcctacgc tcgtcagttc ttgggaaata tggaaaaacc agatgtagat gccattgatg
    11101 gtctcagccc agctatttct atcgaccaga aaacgactag caaaaaccct cgctcgacgg
    11161 tgggaacaac gactgaaatc aatgactatc tgcgtctcct ctacgcgcgt gtggggacgc
    11221 cttactgtat caacgggcat ggggctatca aggcttcttc tgtggagcaa atcgttgata
    11281 aggttttgga gttacctgaa cgccagcgcc tgcagatttt ggcccctgtc atccgtaaga
    11341 aaaaaggaca gcataagagc gttatcgaga agattcagaa agatgggtat gttcgtgtcc
    11401 gtgtggatgg ggaagtctat gatgtgaccg aagtgctaga gttgtccaag agcagacaac
    11461 acaatattga tgtcgtggtt gaccgtattg ttatcaagga gggcattcgt agtcgtctct
    11521 ttgattccat tgaggctgcc cttcgtatcg cagaaggtta tgtgattatc gacaccatgg
    11581 acgactctga gttgttgttc tctgagcatt atgcctgtcc agtttgtgga tttactgtcc
    11641 cagagttaga acctcgtctc ttctctttca atgctccctt tggctcttgt agtgagtgtg
    11701 acggcttggg gattaagctg gaggtggata ctgatttggt agtgccagat gccagcaaaa
    11761 ccttacatga gggagcgctg gcaccttgga atcctatctc atccaactac tatccaaaca
    11821 tgttagagca ggccatgaaa gtctttggag tggctatgga taagcctttt gaggacctgt
    11881 cagaagaaga taagaacttg attctctacg gctcagatgg taaggaattc catttccatt
    11941 atgaaaatga atttggtggt gtgcgcgata tcgacattcc gtttgaggga gttatcaata
    12001 atatcaaacg tcgttaccat gaaaccaata gcgattacac tcgcactcag atgcgtctct
    12061 acatgaatga gctgacctgc ggaacctgtc aaggctatcg tctcaatgac caggccttgt
    12121 ctgtccgtgt gggcggccag caagggccac atattggaga aatctcagac ctgtctatcg
    12181 ctgaccactt ggacttggtg agccagttaa ctttgtctga aaatgaagcc atcattgctc
    12241 gtcccattct caaggaaatc aaggatcgtt tgaccttcct taataatgtg ggtcttaact
    12301 atctaaccct gtcacgttca gcaggaaccc tttcaggtgg ggaaagtcag cgcattcgtt
    12361 tggcaaccca gattggttcc aacctatcag gtgtcctcta tatcctagac gagccgtcaa
    12421 tcggtcttca ccagagagac aatgaccgcc tgattgccag tctgaaaaag atgcgtgact
    12481 tgggcaatac tcttatcgtg gtggaacacg acgaagatac catgcgtgaa gctgattatc
    12541 tgattgacgt tggtcctggt gccggtgttt ttggtggtga gattgttgca gcaggtacac
    12601 ctaagcaagt ggctcgcaac agcaagtcta tcacaggtca gtacttgtca ggcaaacgtg
    12661 tcatcccagt accagaagag cgccgtgtcg gaaatggtcg ttttattgaa gtgataggag
    12721 cgcgtgagaa caacttgcaa aatgtcacgg ctcgctttcc actaggaaaa tttatcgcag
    12781 tgacaggtgt gtcgggttca gggaaatcga ctctaatcaa cagcattctc aaaaaagcca
    12841 ttgcccagaa gctcaaccgt aattcagaca aacctggtaa gtttaagact attacaggga
    12901 ttgagcatgt cgaccgtttg attgatattg accagagccc tatcggacgg acgccgaggt
    12961 ctaacccagc tacctataca ggagtttttg acgatatacg tgaccttttt gctcagacaa
    13021 atgaagccaa gattcgaggc tacaagaagg ggcgcttcag tttcaacgtc aagggaggtc
    13081 gctgtgaagc ttgctcaggt gacgggatta tcaagattga gatgcacttc ttgccagatg
    13141 tttatgtggc ttgtgaagtt tgccacggaa cccgctataa cagtgaaacc ctagaagttc
    13201 actacaagga aaagaatatt tcgcaggtct tggatatgac ggtcaacgat gcggtagaat
    13261 ttttccaaca cattccgaaa attcaacgca aactccagac catcaaggat gtagggctag
    13321 gctatgtgac cttgggacag ccagctacca ccctttctgg gggagaagcc caacgtatga
    13381 agttagctag cgaactccac aaacgctcga caggaaaatc tttctacatt ctggatgagc
    13441 cgacgacagg gcttcataca gaggacattg ctcgcctgct caaggtttta gctcgctttg
    13501 tagacgatgg caatacagtc ctcgtcatcg agcacaatct ggatgttatc aagacggcag
    13561 accatatcat tgacttggga cctgagggcg gtgtcggtgg tggaaccatc atcgtaacag
    13621 gaactccaga agaagtagct gccaacgaag ccagctatac aggacactat ttgaaaggaa
    13681 agttacatca tgaataaacg cgtacaagca tttctagcta aaatgcaaga aaaagaacta
    13741 gatggtatca tcatcaacaa tcttaaaaac gtctattatt tgactggttt ttggggctca
    13801 aacggaacag tctttatcag ccgtgaccgt caggtcttag tgacagactc tcgctatatc
    13861 atcgcagcta agcaagaaac cagtggtttt gagattgtgg ctgatcgtga tgaattggct
    13921 gtcattgcag gaattgttaa ggacatgggc ttgactcgta tcggttttga agatgagatt
    13981 tcagtatctt attaccaccg tatgcaggca gcttttgcag gtttggactt gcttccacaa
    14041 actcaatttg tggaaggtct tcgtatgatt aaggatgagg cagagattgc agcgattcgc
    14101 aaggcttgtt ctatctcaga ccaagctttc cgcgatgcgc ttgactttat caaaccagga
    14161 aaaactgaga ttgaaattgc caacttcctt gatttccgca tgcgtgagtt gggagcatct
    14221 agcttatctt ttgatacgat cctagctagc ggtatcaatt cttctaaacc ccatgcccat
    14281 ccaatgcaca aaccagtgga gttgggagaa gccattacca tggacttcgg ctgtctctat
    14341 gaccactatg tcagtgatat gacccggact atctatctag ggcatgttag cgatgagcag
    14401 gcagagattt acaatacggt tctaaaagct aaccaagcct tgattgacca agctaaggca
    14461 ggcttaggtt tccgtgactt tgacaaaatc cctcgtgata ttatcattga ggcaggttat
    14521 ggcgactact ttactcacgg cattggccac ggtattggtc tggatatcca tgaggaaccc
    14581 tactttagcc agacttctac agaaactatt aagacaggta tggccttgac cgatgaacca
    14641 ggtatctata tcgaaggcaa atatggcgtt cgtatcgagg atgatatcct gattacagag
    14701 acaggttgtg aattattgac cctagctcca aaagagttga tagtcattta gttgtttgtc
    14761 aagaaaaaag tacgaaaatg acgaaaaaag tcaaaaaaaa ttaaaaatag gtcgcaagtc
    14821 ggatgttttt tatggtataa tagactaaac tgatagtaac atgtagcgaa aggggtaggt
    14881 acatgattaa aatttataca gtctcaagtt gtactagctg taaaaaagca aaaacctggc
    14941 tcaatgccca ccagttaagt tataaagaac aaaaccttgg taaagaagga attacgagag
    15001 aagaattact ggatattcta accaaaacag ataacggaat agccagcatc gtttcgtcta
    15061 aaaatcgcta tgccaaagcc cttggagtgg atattgaaga tttgagtgtc aatgaagttc
    15121 tcaatctgat tatggaaaca ccgagaattt taaagagccc aatccttgta gatgaaaaac
    15181 gcctgcaagt tggctacaag gaagacgata ttcgtgcctt cctaccacgc tctgtccgta
    15241 atgtagaaaa tgcagaagca cgtttgcgtg cagctctata aacatcaagg ctgggagcac
    15301 ttcccagtct tattctattt taatttcaaa aagaaagaag aaagaaatga aaaaaatagt
    15361 tcttgttagt ctagctttcc tttttgtcct ggttggttgc ggacagaaaa aagaaactgg
    15421 accagctaca aaaacagaaa aagatacgct tcagtcggca ttgccagtta ttgaaaatgc
    15481 tgagaagaat acagttgtaa ctaagacttt ggtcttgccc aagtcagatg atggtagcca
    15541 gcaaactcaa accattacat acaaggacaa gacttttttg agcttaacga ttcaacaaaa
    15601 acgtccagtt tctgatgagt tgaagactta tatcgaccaa catggagtgg aagaaactca
    15661 aaaagctctt cttgaagcgg aggagaagga taagtctatc attgaagctc gtaaattggc
    15721 aggtttcaaa cttgaaacaa aactattgag cgcaacggaa cttcaaacaa cgactagttt
    15781 tgattttcaa gttctggatg tcaagaaggc ttcccagttg gaacatctga agaatattgg
    15841 ttttgaaaat cttttgaaaa atgaaccaag caaatatatt tcagatagat tggcaaatgg
    15901 cgcgacagaa caatagaaaa tccaaataaa tagactgcct gaattgtaga aggtaaggaa
    15961 ttatgctata atggtactat tctaaggaaa gagggtgtta gaatgggatt tactgaagaa
    16021 acagtacgtt ttaaattgga cgattccaat aaaaaagaaa ttagcgaaac tttgacagat
    16081 gtttatgctt cgttgaacga taagggttac aacccaatta accaaatcgt aggttacgta
    16141 ttgagtggag accctgccta cgttcctcgt tataataatg cacgaaatca aatccgtaag
    16201 tatgagcgtg atgaaatcgt tgaggaattg gttcgttact acctcaaagg acaaggagtc
    16261 gatctataac ctatgagaat tatgggattg gacgtcggtt caaaaacggt aggggtggcg
    16321 attagcgatc cgcttggttt tacagctcaa gggcttgaaa tcatccagat aaatgaagaa
    16381 caaggccaat ttggttttga ccgcgttaag gaattggttg atacttacaa ggtggaacga
    16441 tttgtagtgg gcttgcctaa aaacatgaac aatacaagtg gaccgcgcgt ggaagctagt
    16501 caagcatacg gagcaaagct agaagagttt tttggtttac cagtagacta tcaggatgaa
    16561 cgcttgacaa cagtggctgc tgagcgcatg ttgattgaac aagcagatat cagtcgcaat
    16621 aagcgcaaga aagtcattga taagttagca gctcagctga ttttacaaaa ttatttagat
    16681 agaaaatttt aatataaagg agaggctatg tcacacgatc ataaccacga ccacgaagaa
    16741 cgtgaactaa tcacactagt agatgagcaa ggaaatgaaa ccttgtttga aatccttttg
    16801 acaattgatg gaaaagaaga atttggtaaa aactatgttc ttctagtacc agttaacgca
    16861 gaagaagacg aagacggaca agttgaaatc caagcttact cattcatcga aaacgaagat
    16921 ggaacagaag gcgaattgca accaatccca gaagactcag aagacgaatg gaacatgatt
    16981 gaagaagtct tcaacagctt tatggaggag tgatacagtc tgggagactg tatcagccca
    17041 actcccagaa atggggaaga gttggaacgt ccagtggatg tttttagccc atattcacat
    17101 tcatagtagc tagttattag ctaaccaggt aagaggtcgg gacgaaaatc ctggccttgt
    17161 ttttattagt aaccatactt taatgcgata attgattgga cttttgtcaa ggagatgtgt
    17221 atgtttgaag tagaagaatg gctccatagt cggattggtt tgaattttcg atcaggtttg
    17281 ggtcgaatgc agcaagcggt ggatttgtta ggaaatcctg agcagtctta ccctattatc
    17341 cacgtaacag ggactaatgg gaaaggatct accattgctt ttatgaggga attatttatg
    17401 gggcatggca aaaaagttgc gacctttacc tcccctcata tcgtctctat caatgaccga
    17461 atctgcatta atgggcagcc tatagcagac gcagacttta tccgtttgac tgatcaggtc
    17521 aaggagatgg agaaaacgct tctgcaaact cctgcccagt tgtccttttt tgaattgctg
    17581 accttggttg cttttcttta ttttagggag caggaggtgg atttggtttt attagaagtg
    17641 ggaattggtg gcttacttga cacgaccaat gtggtaactg gagagcttgc tgtcatcacc
    17701 tccattgggc ttgaccatca agaaaccttg ggtgatagtc tagaagcaat tgcagagcag
    17761 aaagctggta ttttcaaggc tggtaagaag gcagtgattg cgaaattgcc tccagaagct
    17821 aggcttgcct gtcagaaaaa agccgaatct ttagctgtta acctttatca ggcaggtcaa
    17881 gattttttaa tgctgaatgg tgatttttca agctctttac taaatctttc acagctgaac
    17941 ataggcttag aaggagtcta tcagcaggag aatgcagcct tggcgttgca aacttttctt
    18001 ctttttatga gagaaagaaa ggaagctgtt gatgaacagg ctgtaagaaa ggccttggaa
    18061 cagacccatt gggctggtcg cttggagcgt attcgcccac agatttattt ggatggtgct
    18121 cataacctcc ctgccttgac tcgcttggct gagtttatca aagaaaaaga gcaggaaggc
    18181 tatcgacctc aaatcctctt tggatccttg aaacgtaagg attatcaagg gatgttgggt
    18241 tatctgactg aaaaattgcc tcaggtggaa ctcaaggtga ccggctttga ctatcagggg
    18301 gctttggacg aaagggatgt gacaggttac gatatagttt cttcttaccg agaatttatc
    18361 agcgattttg aagaaagggc agacgctcaa gacttgctgt tcgttacagg gtctctctat
    18421 tttatctcag aagtacgggg ctacctgctg gaccgtgagc agataaattg actccatttt
    18481 ttgaacttgt tatactaggg gaattgctgg ttagagaaaa tttctctaaa ttggtagcag
    18541 aaaggaaatt catcatgaaa ttaaaaagat tcacactttc tcttgcttct ctagcaagtt
    18601 ttagtctctt agtagcttgt tcacaaagag ctcaacaggt tcaacagcct gttgctcagc
    18661 aggtccaaca gcctgctcaa cagaatacca atactgcaaa tgcaggaggt aaccaaaatc
    18721 aagcggctcc agtacaaaac caacctgttg ctcaaccgac cgatattgat gggacttata
    18781 ctggtcagga tgacggagac cgtatcactt tagtggtaac tggaacgact ggtacatgga
    18841 ctgagctcga atctgacggg gatcagaaag tcaaacaggt tacattggat tcagcaaatc
    18901 aacgcatgat tattggcgat gatgtcaaaa tttacactgt aaacggtaat caaattgtcg
    18961 tagatgatat ggatagagac ccatcggacc aaatcgtttt aactaaataa gactatgcaa
    19021 gtagaaaggg ctggattttt cagccttttt acttttacct agaaaataat cataaatact
    19081 tgccttctac cgaatacctg cgttatacta gtatcaatta cttgttttta gcattcaaac
    19141 tatcaaggag gggatatgaa atataggaaa tttcaattat tgatgtccaa gtatggcttt
    19201 agtctttcga ttatgctact tgaactttgt cttgtttttg gtctctttct ttatttagga
    19261 cgcatggctc ccattttatg gattactgtc ctcattctac tgagtatcat cacaatcatt
    19321 tcgatagtca accgtaatac gactcctgag aataaggtaa cctggttgtt agtagccttt
    19381 gtgccagtat ttggtccctt gctctatctg atgtttggtg aaaggcgatt gtccaaaaaa
    19441 gaaatcaaac aactgaagaa gctaggctct atgcatttcc aagaagcaaa tagccagcta
    19501 ctaaaagaga aattaaaaga aagtgacaag gcagcttatg gagtcatcaa gtccttattg
    19561 agtatggata ccaatgctga catctatgat caaactgcct ctacattttt tcctaacgga
    19621 gaagctatgt ggaaaaagat ggtagaagat cttaaaaagg ctgagaaatt tattttcttg
    19681 gaatattaca ttatagaaga aggtttgatg tggaatcgca tactagatat actagagcaa
    19741 aaggtagctc agggtgtaga ggttaagatg ctctatgatg atatcggctg tatggctact
    19801 ttaacaggag attatgcaca tcgacttcgt cagctgggca tcgaggccca taaattcaat
    19861 aaagttattc ctcgtttgac agtggcttat aataacagag atcatagaaa aatattgatt
    19921 gttgatggtc agatagccta tactggtggg gtcaatctgg cagatgagta cattaaccac
    19981 gtcgagagat ttggttattg gaaggatagt ggaattcgct tagacggact agcagtaaaa
    20041 gccctgacac gcttattttt gaccacttgg tacattaatc gaggagaaat tagtgatttt
    20101 gatcaatatc atttagaaaa tcattctatc ccgagtgacg gtttaaccat tccatacgga
    20161 agtggaccca agccaatttt tcgagcgcag gtagggaaaa aagtttatca gagtttaatc
    20221 aatcaagcaa cagaatcggt ctatattacg acaccttatt tgattataga ttatgattta
    20281 acagagacaa tcaaaaatgc agctatgaga ggggtcgatg ttcgaattat caccccttac
    20341 ataccagata agaagttcat tcagttagtc acgagaggag cttatcccga ccttctttct
    20401 gctggtgttc ggatttatga gtatagtcca ggttttattc atagtaagca gatgttggta
    20461 gacgaagatt ttgcggtggt ggggacaatc aatctcgact accggagctt ggtacaccat
    20521 tatgaaaatg cagtcttact ctataaaact ccttctataa gggaaatcgc ccgagatttt
    20581 cgaaatatat ttgcagattc tcaggaagtc tatcctcatt ctatcaaaac gagctggtat
    20641 caaaagcttg taaaagaaat cgcccagcta ttcgccccta tcttataaga aatctagaac
    20701 ttaggataca aaccagacct atttttcttg tcttttacga atagaaagaa agaggagggg
    20761 aattcatgct aactaaggaa gaagtgaatg atatgattga gttcaaacag acacatttac
    20821 accgctttcg ggaaattgaa acctttgtga aactatgcaa aaaatcactc aaacagccat
    20881 cgcaagctga caatcctagg actttttgat atactaagac agataaattg aacaggagaa
    20941 acgatatgag tgtgtatggt agagtagaag aagttcataa ggagaatcgt gaacctctag
    21001 aataccaaat cgaacaagaa tcgcatcatc gtgaatcaag tcgtcttcct ttggtgaaaa
    21061 ttttactatg gagtacgctt gtaacgggga taactctagg agtaccgcta ttactcgact
    21121 taatgagtgc acaagaagtg caggattttt atgcaggttg ggcgcttcat cagacaggga
    21181 agatttacag caactattat ggaagtcaag gtttgcttta ttatttgctg acttacgtga
    21241 gtcagggcgg atttttcttt gccatctttg agtggttagc cttggtagca ggaggatttt
    21301 tcctttttag atcagcggac accttgacag agcaaggaga ccaagctgga catctggtga
    21361 ctatttttta catgctagtt acaggtcttg cttttggtgg aggctatgcg actcttttag
    21421 cgcttccttt cttattcgca gcctttagtt tagttgcggc ttacctaagc aatccaagcc
    21481 atgataaggg atttgtacgg attgggctag ctttggcagg cggatttttc tttgctccct
    21541 tatcatcgct cctgtttatt gctgtagtga gtttaggctt gttggtcttt aaccttgggc
    21601 atagacgctt tgcgcatggg ttttatcagt ttcttgcagt ggctttaggt ttttcacttg
    21661 tcttttatcc aactgcctac tatagtgctg caacaggaag ttttggggat gcgattagtg
    21721 gtattcgtta tcctattgac agtattcgct ttgattttac ttctaaaatt ttagagaata
    21781 tgttttttta tggcttgttg tcccttggtt tgggatttgt ggtttgtatc tttttaggtc
    21841 tctttcaatc caaacccttt aaactatacg tcatttcggt tcctgcaagt cttgtggtaa
    21901 ttttaggttt gattttactt ttcttttcac aagagcctct gcacgcttct tatttgatgg
    21961 tcgtcttccc tgttttccta cttttattgg taaccaatat taagagtcaa cagagggggc
    22021 gtagtgctag aagaagccga agagaaacgc cagttagcct atggagtcgt ttcttcaaag
    22081 gaaatctata tctgctagtt tttgggtttg tctatctttt gtctgttcct tttttgatga
    22141 agtttgtcct ttatccagta ccttatcaag aacgtaatcg tcttgctgat ttggtaaaag
    22201 aggagacaaa tacggaagat gctatctatg catgggatga tactgcgact ctttatcgta
    22261 agagtgagcg cttgtcgcca tcggcgattt tgtccccgtt gcactataca gcaactgagg
    22321 aaaatcgtaa taagttactt aatgacttga aagaaaaaca acctaaggtg attgtggtaa
    22381 atgacaaggt ggaagtctgg tctgaagtgg aaacactctt aaaagaaaat taccaacaag
    22441 taaagactga ttactcagag tttaaagtct ataaaattaa ataaccaaat caatatcttg
    22501 tgtattttta aaaattttaa gatttttaac acaagatatt gatttttctt tttagagtgg
    22561 tataatactt tttagaaaga acattttaga aaagagcatg cgtatgattg cactagaaga
    22621 aaaaattaca attttgccaa ctctcttcgt cgagaaacga gatgggagac gtgttgtatt
    22681 tgatgtggac aagattgaca aggctctcca caaggcggct gacaaggtta tggatgtgac
    22741 acccctggtt gaaaaacgcc tcaatgatct gactgagcga attattacag aaattcatag
    22801 tcgctttcca cagggaatta agatttacga aattcaaaat atcgtagaac atgaactcct
    22861 tgaagccaaa gaatatgcgc tggctgagga gtatattact tatcggacac agagggattt
    22921 tgagcgctca aaagcgacgg atatcaactt tagtattcat aaacttctca acaaagacca
    22981 gacagttgtc aatgaaaatg ctaataaaga cagtgatgtc tttaacactc agcgtgattt
    23041 gacagcaggg attgttggga aatcaatcgg actgcaaatg cttcctaagc acgtagccaa
    23101 tgcccaccaa aagggggata tccactatca cgatttggac tacagtccct atacccctat
    23161 gaccaactgc tgtttgattg attttaaggg tatgttggaa aatggtttta agattggaaa
    23221 tgcagaggta gagagtccca agtctatcca gactgcgaca gcacagattt ctcaaatcat
    23281 tgccaacgtt gcttctagcc agtacggtgg ctgttcagct gaccgtatcg atgaaatttt
    23341 ggcgccttat gcagagaaga actatcaaaa acatctcaaa gatgcagaag agtgggtatt
    23401 gcctgaaaaa cgggaagatt acgcttggaa gaaaacgcaa aaggacatct acgatgccat
    23461 gcaatctctt gagtatgaaa tcaatactct ctttacttca aatggacaaa caccttttac
    23521 ttcgctaggt tttggtctgg gaaccagtcg ttttgaacga gaaattcaaa aagctatttt
    23581 aaacattcgc atcaagggtc ttggttcaga acaccgtacg gctatctttc ctaaacttat
    23641 tttcactctg aaaagaggcc ttaatttaga ggaaggaact cccaactacg acatcaagca
    23701 gttggctctt gagtgtgcaa ccaaacggat gtatccagat gttttatctt acgataagat
    23761 tgttgacttg acaggatcct tcaaggtgcc tatgggttgc cgttctttcc tccaaggatg
    23821 gaaggatgaa aatggtgtag aagtcaattc aggtcgcatg aatctgggtg ttgtgacggt
    23881 taatctgcct cgtattgctc ttgagtctga aggtgatatg aataagttct gggaaatctt
    23941 caacgagcga atgaatatcg cagaagatgc tcttgtttac cgtgtcgaac gcactaaaga
    24001 ggcgacacca gcgaatgctc ctatccttta tcagtacggt gcttttggcc atcgtctagg
    24061 taaagaagaa agtgttgacc agctctttaa gaatcgtcgt gcgaccgttt cgctgggcta
    24121 tatcggcttg tatgaagtag cgacagtttt ctttggtaac agctgggaaa gtaatccaga
    24181 tgctaaggaa ttcacgctag acatcattca cgatatgaaa cgccgtgtag aagagtggtc
    24241 aaaccaatat ggctaccatt tctctatcta ctcaacacca tccgaaagtc tgacagaccg
    24301 tttctgccga ctagatatag acaagtttgg ctctattcct gatatcacag acaaggaata
    24361 ctacaccaac tctttccact acgatgttcg taaaaatcca acaccgtttg aaaaattgga
    24421 ctttgagaaa gtctatccag aagcaggtgc gtcaggtggt ttcatccatt attgtgagta
    24481 tccagtcctt cagcaaaatc caaaggcctt ggaagctgtc tgggattatg cttatgaccg
    24541 tgtaggctat ctaggcacca atactccgat tgaccgttgc tacaagtgtg actttgaagg
    24601 ggattttgaa ccaactgaga gagggtttgc ttgtccaaac tgtggcaata gcgaccctaa
    24661 aacagtagat gtggtgaagc gaacttgtgg ctacctaggt aatcctcaag caagaccgat
    24721 ggtcaacggg cgtcacaagg aaatcgctgc gcgtgtcaaa catatgaatg gttcaacgat
    24781 taaaatagct gggcatcaag taacaaatta gaaagaaatg aaatgggaaa atatcaacta
    24841 gacgataagg ggcgcgcaca agtgacccgt tatcacgaga aacactctaa aggtggagct
    24901 ggtaagaaag aacgcttgct tagcttcaga gaacaatttt taaacaagaa caagaaaaaa
    24961 taaaagtgag agccagctct cgcttttctc atagtgggag gtaaggatgg aattacgcag
    25021 accaagatta gcggataaga aagctgtttt agatatgatg acagagtttg aaaaatttca
    25081 gtcgcctcac gacggcggtt tctgggatac agagaacttt gtgtatgaag actggttaga
    25141 aagcaatcag gaacaggaaa tggggattaa tctgcctgaa ggatgggttc ctgcaattca
    25201 gttagtggct ttttctgaga aaggtcaagc agttggattt cttaatctcc ggttgcgcct
    25261 cagtaacttt ctactagaag aaggtggcca cattggctac tccattcgtc catctgaaag
    25321 aggcaagggt tatgcaaaag agactctccg tcagggcttg caagttgcta aggaaaagaa
    25381 catcaagaaa gctctggtga cctgtagcgt gaataatcct gctagcagag cagccattct
    25441 agcaaatggt ggaatatttg aggatgctcg caatggagtc gagcgttatt ggatagaggt
    25501 agcgaatgaa taatccaaaa ccacaagaat ggaaaagcga ggaacttagt caaggtcgta
    25561 tcattgacta caaggccttt aactttgtgg acggcgaagg cgtgcgcaac tctctctatg
    25621 tatcaggctg tatgtttcac tgtgagggat gttataatgt tgcgacttgg tcttttaatg
    25681 ctggcattcc ctatacagca gaattagaag agcagattat ggcagacctt gcccaaccct
    25741 atgttcaagg cttgactttg ctgggagggg agccttttct caatactggc attctcttgc
    25801 ctctagttaa acgcatccga aaggaattgc cagacaagga catttggtcc tggacgggct
    25861 acacttggga agaaatgatg ttggaaactc cagataaact ggaactcttg tcactgattg
    25921 acattcttgt cgatggacga tacgatcgaa ctaagagaaa tctcatgctc cagtttcgag
    25981 gttcgtccaa ccaacgaatt atcgatgtgc aaaaatcgct caaaagtggg caagtagtga
    26041 tttgggacaa gctcaatgac ggaaaagaaa gctatgaaca ggtgaagaga gaatgaagaa
    26101 aaaggactta gtagaccaac tagtctcaga gatcgagacg gggaaagtca ggacactggg
    26161 aatatacggt catggagctt caggtaaatc aacctttgca caggaattgt accaagcttt
    26221 agattctact acagtaaatt tgctagagac agatccttat atcacctcag gacgccatct
    26281 ggtagtaccc aaggacgcgc cgaatcaaaa ggtgacagcc agtctgccag tggcgcatga
    26341 actggagagt ttgcagagag atatccttgc cttgcaggcg ggtatggatg tcttgacaat
    26401 tgaagaacct tggaaggcta gtgaggtctt gtctggagcc aaaccaattt tgattgtcga
    26461 agggatgtct gttggctttc tacccaagga actctttgaa aaaccatctg tttctacacg
    26521 gatgaggaga ccgaattaaa gcgacgcctt gctagagata cgactgtgag aaatcgcgat
    26581 gcatccttta tattggctag ccatcagatg agacgggagc agtatctgcg atactataaa
    26641 gaaactgagt ctaaggcgga tatcttagtg gaccaatcag aagataaatt tgatgtcaag
    26701 aggacttaac tatagaagaa atatagaaga aaatccctaa ttttagaaag aaaaagtagg
    26761 aaaacagttt catcccaaaa aaacgaaaaa acctaacaaa tcccttgcaa tcgcaggggc
    26821 tttgtgttat tctattatgg tgctgtaaat tacagcctta gttttgatgc aagaggttgc
    26881 gacacgctcg gttgcattgc cacgcaacac cgcgtcggtt ttcttgtgga gctagcctat
    26941 tatcttaaat agacgaaaag gagaaaaaga tggcaaacaa aaaaatccgt atccgtttga
    27001 aagcttacga acaccgtacg cttgacacag cggctgcaaa aatcgtagaa tcagctactc
    27061 gtacaggtgc acaagttgcg ggtccaatcc cacttccaac tgaacgtagc ctctacacaa
    27121 tcattcgtgc gactcacaaa tacaaagact ctcgcgaaca atttgaaatg cgtacacaca
    27181 aacgtttgat cgatatcgtt aacccaactc aaaaaacagt tgatgccttg atgaaattgg
    27241 atcttccaag tggtgtaaac gtagaaatca aactttaatc taaaatataa aagagcagag
    27301 gctggtgttt caatctaatt gaacacgggc taaactcggt gtgaaaaaga taaacttcct
    27361 cgcgtctgct agacactgtg tcagtttcct attttcacgt tgagtttgac gccctttgta
    27421 tcttagactt gagcataaaa aacgctcgtt aaaaactttt tgaataaaaa tatagaaaag
    27481 gaactatttt ctcatgacaa aaggaatctt agggaaaaaa gtgggaatga ctcaaatctt
    27541 cactgaagct ggcgaattga tccctgtaac agttattgaa gcaactccaa acgttgttct
    27601 tcaagttaaa actgttgaaa cagacggata caacgctatc caagttggtt tcgatgacaa
    27661 acgcgaagta ttgagcaaca aacctgctaa aggacatgta gcgaaagcta acacggctcc
    27721 taagcgcttc attcgtgaat tcaaaaacgt tgaaggcttg gaagttggtg ctgaaattac
    27781 agttgaaaca ttcgcagctg gagacgttgt tgacgtaact ggtacttcta aaggtaaagg
    27841 tttccaaggt gttatcaaac gccacggaca atcacgtgga ccaatggctc acggttctcg
    27901 ttaccaccgt cgtccaggtt ctatgggacc tgttgcacct aaccgcgtat tcaaaggtaa
    27961 aaaccttgca ggacgtatgg gtggtgaccg cgtaacaatt caaaatcttg aagttgtaca
    28021 agttgttcca gaaaagaacg ttatccttat caaaggtaac gtaccaggtg ctaagaaatc
    28081 tcttatcact atcaaatcag cagttaaagc tggtaaataa taaagaaagg ggaaatcagt
    28141 cacaatggca aacgtaacat tatttgacca aactggtaaa gaagctggcc aagttgttct
    28201 tagcgatgca gtatttggta tcgaaccaaa tgaatcagtt gtgtttgatg taatcatcag
    28261 ccaacgcgca agccttcgtc aaggaacaca cgctgttaaa aaccgctctg cagtatcagg
    28321 tggtggacgc aaaccatggc gtcaaaaagg aactggacgt gctcgtcaag gttctatccg
    28381 ctcaccacaa tggcgtggtg gtggtgttgt cttcggacca actccacgtt catacggcta
    28441 caaacttcca caaaaagttc gtcgcctagc tcttaaatca gtttactctg aaaaagttgc
    28501 tgaaaacaaa ttcgtagctg tagacgctct ttcatttaca gctccaaaaa ctgctgaatt
    28561 tgcaaaagtt cttgcagcat tgagcatcga ttctaaagtt cttgttatcc ttgaagaagg
    28621 aaatgaattc gcagctcttt cagctcgtaa ccttccaaac gtgaaagttg caactgctac
    28681 aactgcaagt gttcttgaca tcgcaaatag cgacaaactt cttgtcacac aagcagctat
    28741 ctctaaaatc gaggaggttc ttgcataatg aatttgtatg atgttatcaa aaaacctgtc
    28801 atcactgaaa gctcaatggc tcaacttgaa gcagggaaat atgtatttga agttgacact
    28861 cgtgcacaca aacttttgat caagcaagct gttgaagctg ctttcgaagg tgttaaagtt
    28921 gccaatgtta acacaatcaa cgtaaaacca aaagctaaac gtgttggacg ttacactggt
    28981 tttactaaca aaactaaaaa agctatcatc acacttacag ctgattctaa agcaatcgag
    29041 ttgtttgctg ctgaagctga ataatctaag gaggaaatat cgtgggaatt cgtgtttata
    29101 aaccaacaac aaacggtcgc cgtaatatga cttctttgga tttcgctgaa atcacaacaa
    29161 gcactcctga aaaatcattg cttgttgcat tgaagagcaa ggctggtcgt aacaacaacg
    29221 gtcgtatcac agttcgtcac caaggtggtg gacacaaacg tttctaccgt ttggttgact
    29281 tcaaacgtaa taaagacaac gttgaagcag ttgttaaaac aatcgagtac gatccaaacc
    29341 gttctgcaaa catcgctctt gtacactaca ctgacggtgt gaaagcatac atcatcgctc
    29401 caaaaggtct tgaagtaggt caacgtatcg tttcaggtcc agaagcagat atcaaagtcg
    29461 gaaacgctct tccacttgct aacatcccag ttggtacttt gattcacaac atcgagttga
    29521 aaccaggtcg tggtggtgaa ttggtacgtg ctgctggtgc atctgctcaa gtattgggtt
    29581 ctgaaggtaa atacgttctt gttcgtcttc aatcaggtga agttcgtatg attcttggaa
    29641 cttgccgtgc tacagttggt gttgtcggaa acgaacaaca tggacttgta aaccttggta
    29701 aagcaggacg tagccgttgg aaaggtatcc gcccaacagt tcgtggttct gtaatgaacc
    29761 ctaacgatca cccacacggt ggtggtgaag gtaaggcacc agttggtcgt aaagcaccat
    29821 ctactccatg gggcaaacct gctcttggtc ttaaaactcg taacaagaaa gcgaaatctg
    29881 acaaacttat cgttcgtcgt cgcaacgaga aataatatta aactagtcgc ttaagcaact
    29941 agtaaatccg ccagctcggt agcgctccat aggagtgcaa gccgctgtgg tacaacattt
    30001 aaaggagaaa atataaaaat gggacgcagt cttaaaaaag gacctttcgt cgatgagcat
    30061 ttgatgaaaa aagttgaagc tcaagctaac gacgaaaaga aaaaagttat taaaacttgg
    30121 tcacgtcgat caacgatctt cccaagtttc attggttaca ctattgcagt ttatgacgga
    30181 cgtaaacacg tacctgttta catccaagaa gacatggtag gccacaaact tggtgaattt
    30241 gcaccaactc gtacttacaa aggtcacgct gcagacgaca agaaaacacg tagaaaataa
    30301 ggagaacata aatggcagaa attacttcag ctaaagcaat ggctcgtaca gtacgtgttt
    30361 cacctcgtaa atcacgtctt gttcttgata acatccgtgg taaaagcgta gccgatgcaa
    30421 tcgcaatctt gacattcact ccaaacaaag ctgctgaaat catcttgaaa gttttgaact
    30481 cagctgtagc taacgctgaa aacaactttg gtttggataa agctaacttg gtagtatctg
    30541 aagcattcgc aaacgaagga ccaactatga aacgtttccg tccacgtgcg aaaggttcag
    30601 cttcaccaat caacaaacgt acagctcaca tcactgtagc tgttgcagaa aaataaggag
    30661 gtaaaatcgt gggtcaaaaa gtacatccaa ttggtatgcg tgtcggcatc atccgtgatt
    30721 gggatgccaa atggtatgct gaaaaagaat acgcggatta ccttcatgaa gatcttgcaa
    30781 tccgtaaatt cgttcaaaaa gaacttgctg acgcagcagt ttcaactatt gaaatcgaac
    30841 gcgcagtaaa caaagttaac gtttcacttc acactgctaa accaggtatg gttatcggta
    30901 aaggtggtgc taacgttgat gcactccgtg caaaacttaa caaattgact ggaaaacaag
    30961 tacacatcaa catcatcgaa atcaaacaac ctgatttgga tgctcacctt gtaggtgaag
    31021 gaattgctcg tcaattggag caacgtgttg ctttccgtcg tgcacaaaaa caagcaatcc
    31081 aacgtgcaat gcgtgctgga gctaaaggaa tcaaaactca agtatcaggt cgtttgaacg
    31141 gtgcagatat cgcccgtgct gaaggatact ctgaaggaac tgttccgctt cacacacttc
    31201 gtgcagatat cgattacgct tgggaagaag cagatactac atacggtaaa cttggtgtta
    31261 aagtatggat ctaccgtggt gaagttcttc cagctcgtaa aaacactaaa ggaggtaaat
    31321 aaccaatgtt agtacctaaa cgtgttaaac accgtcgtga gttccgtgga aaaatgcgcg
    31381 gtgaagcaaa aggtggaaaa gaagtagcat tcggtgaata cggtcttcaa gctacaacta
    31441 gccactggat cactaaccgc caaatcgaag ctgctcgtat cgccatgact cgttacatga
    31501 aacgtggtgg taaagtttgg attaaaatct tcccacacaa atcatacact gctaaagcta
    31561 tcggtgtgcg tatgggatct ggtaaagggg cacctgaagg ttgggtagca ccagttaaac
    31621 gtggtaaagt gatgttcgaa atcgctggtg tatctgaaga gattgcacgt gaagcgcttc
    31681 gacttgctag ccacaaattg ccagttaaat gtaaattcgt aaaacgtgaa gcagaataag
    31741 gagaaggcat gaaacttaat gaagtaaaag aatttgttaa agaacttcgt ggtctttctc
    31801 aagaagaact cgcgaagcgc gaaaacgaat tgaaaaaaga attgtttgaa cttcgtttcc
    31861 aagctgctac tggtcaattg gaacaaacag ctcgcttgaa agaagttaaa aaacaaatcg
    31921 ctcgtatcaa aacagttcaa tctgaagcga aataatagac tagggaagga gaaatttcaa
    31981 tggaacgcaa taatcgtaaa gttcttgttg gacgtgttgt atctgacaaa atggacaaga
    32041 caatcacagt tgtagttgaa acaaaacgta accacccagt ctatggtaaa cgtattaact
    32101 actctaaaaa atacaaagct catgatgaaa acaatgttgc caaagaaggc gatatcgtac
    32161 gtatcatgga aactcgcccg ctttcagcta caaaacgttt ccgtcttgta gaagttgttg
    32221 aagaagcggt catcatctaa tcaaacctga aaggagaaaa ctgaaatgat tcaaacagaa
    32281 actcgtttga aagtcgcaga caacagcggt gctcgcgaaa tcttgactat caaagttctt
    32341 ggtggttcag gacgtaaatt tgcaaacatc ggtgatgtta tcgtggcatc tgtaaaacaa
    32401 gctactcctg gtggtgcggt taaaaaaggt gacgttgtta aagcagttat cgttcgtact
    32461 aaatcaggtg ctcgtcgtgc tgatggttca tacatcaaat ttgacgaaaa cgcagcagtt
    32521 atcatccgtg aagacaaaac tcctcgcgga acacgtatct ttggcccagt tgcacgtgaa
    32581 ttgcgtgaag gtggcttcat gaagatcgtg tcacttgctc cagaagtact ttaattttta
    32641 ggaacaaact agtcccctag cttcaagcta gggtgccctt atgggcgtaa gaaaaatcaa
    32701 ggagaaacct aatgtttgta aaaaaaggcg acaaagttcg cgtaatcgct ggtaaagata
    32761 agggaacaga agctgttgtc cttactgccc ttccaaaagt aaacaaagtt atcgttgaag
    32821 gtgttaacat tgttaagaaa caccaacgtc caactaacga gcttcctcaa ggtggtatca
    32881 tcgagaaaga agcagctatc cacgtatcaa acgttcaagt tttggacaaa aatggtgtag
    32941 ctggtcgtgt tggttacaaa tttgtagacg gtaaaaaagt tcgctacaac aaaaaatcag
    33001 gcgaagtgct tgattaatca cgaaggaaag gagaagtata atggcaaatc gtttaaaaga
    33061 aaaatatctt aatgaagtag ttcctgcttt gacagaacaa ttcaactact catcagtgat
    33121 ggctgtgcct aaagtagata agattgtttt gaacatgggt gttggtgaag ctgtatcaaa
    33181 cgctaaaagc cttgaaaaag ctgctgaaga attggcactt atctcaggtc aaaaaccact
    33241 tatcactaaa gctaaaaaat caatcgccgg cttccgtctt cgtgaaggtg ttgcgatcgg
    33301 tgcaaaagtt acccttcgtg gtgaacgtat gtacgaattc ttggataaat tggtatcagt
    33361 ttcacttcca cgtgtacgtg acttccacgg tgttccaaca aaatcatttg atggacgcgg
    33421 gaactacaca cttggtgtga aagaacaatt aatcttccca gaaatcaact tcgatgacgt
    33481 tgacaaaact cgtggtcttg acatcgttat cgtaacaact gctaacactg acgaagagtc
    33541 acgtgcattg cttacaggcc ttggaatgcc ttttgcaaaa taatatagga ggtaaatcta
    33601 atggctaaaa aatcaatggt agctagagag gctaaacgcc aaaaaattgt tgaccgttat
    33661 gctgaaaaac gtgctgcatt aaaggcggca ggggactacg aaggtttatc taaattacct
    33721 cgcaacgcct caccgactcg tttacataat cgttgtagag ttacggggcg cccacattca
    33781 gtttaccgca aatttggtct gagtcgtatc gcttttcgcg aacttgcgca taaaggtcaa
    33841 attcctggtg taacaaaagc atcttggtaa tttaagatat caagagcgtc aaaactccaa
    33901 gtaaaaatag gaaacttgac gaagaaacta aagtttctag gaaagtttat ctttttcaca
    33961 cagagtttag cccgggttca attgggcttg ccaatttgaa cacgagctac agctttggca
    34021 aaaaagacca atttgctttg gagcattgct tctgcattaa attgtctatt tttgctcgtg
    34081 ctgttacgct ctttgtatca tgtattaact agcaagtgca acttgcaaac tactagtaag
    34141 aggagaaaaa caaaatggtt atgactgacc caatcgcaga cttcctaact cgtattcgta
    34201 atgctaacca agctaaacac gaagtacttg aagtacctgc atcaaacatc aaaaaaggga
    34261 ttgctgaaat ccttaaacgc gaaggttttg taaaaaacgt tgaaatcatt gaagatgaca
    34321 aacaaggcgt catccgtgta tttcttaaat acggaccaaa tggtgagaaa gttatcacta
    34381 acttgaaacg tgtttctaaa ccaggacttc gtgtctacaa aaaacgtgaa gaccttccaa
    34441 aagttcttaa cggacttgga attgccatcc tttcaacttc tgaaggtttg cttactgata
    34501 aagaagcacg ccaaaagaat gttggtggtg aggttatcgc ttacgtttgg taaaatcaag
    34561 atacaaagct cgtaaagaac aaagcaaaat taggaagttg gagaagtttg tttacaaaca
    34621 agccaactta tctattttgc acagttctta gagcgtgttc agttcagctc ttgaactaaa
    34681 taagtatctg aaccccgtga aaactggccg ttctggcctg acaatctaac aggagaaaat
    34741 aaacatgtca cgtattggta ataaagttat cgtgttgcct gctggtgttg aactcgctaa
    34801 caatgacaac gttgtaactg taaaaggacc taaaggagaa cttactcgtg agttctcaaa
    34861 agatattgaa atccgtgtgg aaggtactga aataactctt caccgtccaa acgattcaaa
    34921 agaaatgaaa actatccacg gaactactcg tgcccttttg aacaacatgg ttgttggtgt
    34981 atcagaagga ttcaagaaag aacttgaaat gcgtggggtt ggttaccgtg cacagcttca
    35041 aggatctaaa cttgttttgg ctgttggtaa atctcatcca gacgaagttg aagctccaga
    35101 aggaattact tttgaacttc caaacccaac aacaatcgtt gttagcggaa tttcaaaaga
    35161 agtagttggt caaacagctg cttacgtacg tagccttcgt tcaccagaac catataaagg
    35221 taaaggtatc cgttacgttg gtgaattcgt tcgccgtaaa gaaggtaaaa caggtaaata
    35281 atgttgagtg gttgatcatc aaccaccaac ctattttcca actttgtgca tagcacacga
    35341 tttaaaacta aagaggtgaa aactgtgatt tcaaaaccag ataaaaacaa actccgccaa
    35401 aaacgccacc gtcgcgttcg cggaaaactc tctggaactg ctgatcgccc acgtttgaac
    35461 gtattccgtt ctaatacagg catctacgct caagtgattg atgacgtagc gggtgtaacg
    35521 ctcgcaagtg cttcaactct tgataaagaa gtttcaaaag gaactaaaac tgaacaagcc
    35581 gttgctgtcg gtaaactcgt tgcagaacgt gcaaacgcta aaggtatttc agaagtggtg
    35641 ttcgaccgcg gtggatatct atatcacgga cgtgtgaaag ctttggctga tgcagctcgt
    35701 gaaaacggat tgaaattcta ataggaggac actagaaaat ggcatttaaa gacaatgcag
    35761 ttgaattaga agaacgcgta gttgctgtca accgtgttac aaaagttgtt aaaggtggac
    35821 gtcgtcttcg tttcgcagct cttgttgttg ttggtgacca caatggtcgc gtaggatttg
    35881 gtactggtaa agctcaagaa gttccagaag caatccgtaa agcagtagat gatgctaaga
    35941 aaaacttgat cgaagttcct atggttggaa caacaatccc acacgaagtt ctttcagaat
    36001 tcggtggagc taaagtattg ttgaaacctg ctgtagaagg ttctggagtt gccgctggtg
    36061 gtgcagttcg tgccgttgtg gaattggcag gtgtggcaga tattacatct aaatcacttg
    36121 gttctaacac tccaatcaac attgttcgtg caactgttga aggtttgaaa caattgaaac
    36181 gcgctgaaga aattgctgcc cttcgtggta tttcagtttc tgatttggca taagaaaggg
    36241 gataaaatgg ctcaaattaa aattactttg actaagtctc caatcggacg cattccatca
    36301 caacgtaaaa ctgttgtagc acttggactt ggcaaattga acagctctgt tattaaagaa
    36361 gataacgctg ctatccgtgg tatgattaca gcagtatctc acttagtaac agttgaagaa
    36421 gtaaactaat gaagttttag gggatgtgca ctgtaccatc ccctaaaact agatatagtc
    36481 atctatgatg acatcgtata ggcgagttga tgggggagac aaccttttct cccttatcgg
    36541 cgctagcatt ttacaaaaga ggagaaaata aaaatgaaac ttcatgaatt gaaacctgca
    36601 gaaggttctc gtaaagtacg taaccgcgtt ggtcgtggta cttcatcagg taacggtaaa
    36661 acatctggtc gtggtcaaaa aggtcaaaaa gctcgtagcg gtggcggagt tcgccttggt
    36721 tttgaaggtg gacaaactcc attgttccgt cgtcttccaa aacgtggatt cactaacatc
    36781 aacgctaaag aatacgcaat tgtgaacctt gaccaattga acgtctttga agatggtgct
    36841 gaagtaactc cagttgttct tatcgaagca ggaattgtta aagctgaaaa gtcaggtatt
    36901 aaaattcttg gtaacggtga gttgactaag aaattgactg tgaaagcagc taaattctct
    36961 aaatcagctg aagaagctat cactgctaaa ggtggttcag tagaagtcat ctaagagagg
    37021 tgacctatgt tttttaaatt attaagagaa gctcttaaag tcaagcaggt tcgatcaaaa
    37081 attttattta caatttttat cgttttggtc tttcgtatcg gaactagcat tacagttcct
    37141 ggtgtgaatg ccaatagctt gaatgcttta agtggattat ccttcttaaa catgttgagc
    37201 ttggtgtcgg ggaatgccct aaaaaacttt tcgatttttg ccctaggagt tagtccctat
    37261 atcaccgctt ctattgttgt ccaactcttg caaatggata ttttacccaa gtttgtagag
    37321 tggggtaaac aaggggaagt aggtcgaaga aaattgaatc aagctactcg ttatattgct
    37381 ctagttctcg cttttgtgca atctatcggg attacagctg gttttaatac cttggctgga
    37441 gctcaattga ttaaaactgc tttaactcca caagtttttc tgacgattgg tatcatctta
    37501 acagctggta gtatgattgt cacttggttg ggtgagcaaa ttacagataa gggatacgga
    37561 aacggtgttt ccatgattat ctttgccggg attgtttcct caattccaga gatgattcag
    37621 ggcatctatg tggactactt tgtgaacgtc ccaagtagcc gtatcacttc atctatcatt
    37681 ttcgtaatca ttttgattat tactgtattg ttgattattt actttacaac ttatgttcaa
    37741 caagcagaat acaaaattcc aatccaatat actaaggttg cacaaggtgc tccatctagc
    37801 tcttaccttc cgttaaaggt aaaccctgct ggagttatcc ctgttatctt tgccagttcg
    37861 attactgcag cgcctgcggc tattcttcag tttttgagtg ccacaggtca tgattgggct
    37921 tgggtaaggg tagcacaaga gatgttggca actacttctc caactggtat tgccatgtat
    37981 gctttgttga ttattctctt tacattcttc tatacgtttg tacagattaa tcctgaaaaa
    38041 gcagcagaga gcctacaaaa gagtggtgcc tatatccatg gagttcgtcc tggtaaaggt
    38101 acagaagaat atatgtctaa acttcttcgt cgtcttgcaa ctgttggttc cctcttcctt
    38161 ggtgtgattt ccattttacc gattgcagct aaagatgtat ttggtctttc tgatgttgtt
    38221 gcctttggtg gaacaagtct cttgatcatt atctctacag gtatcgaagg aatcaagcaa
    38281 ttggaaggtt acctattgaa acgtaagtat gttggtttca tggacagaac agaataaaag
    38341 tatttactga atcagtaaat gctgagggag tggaggttta aactctgaca tttgtaagag
    38401 ttggatctcc cctcttctat tttgttttta aataggggtg aaaagacttt ttgcttctat
    38461 ttaaaaataa aataaggaga tcaaatcatg aatcttttga ttatgggctt acctggtgca
    38521 ggtaagggaa ctcaagcagc aaaaatcgta gaacaattcc atgttgcaca tatctcaaca
    38581 ggtgatatgt tccgcgctgc aatggcaaat caaactgaaa tgggtgttct tgctaagtca
    38641 tatattgaca agggtgaatt ggttcctgac gaagttacaa atggaatcgt aaaagaacgc
    38701 ctttcacaag atgatattaa agaaacagga ttcttattgg atggttaccc acgtacaatt
    38761 gaacaagctc atgccttgga caaaacattg gctgaacttg gcattgaact agaaggtgtt
    38821 atcaatattg aagtgaactc tgacagcctc ttggaacgtt tgagtggccg tatcatccac
    38881 cgcgtaactg gagaaacttt ccacaaggtc tttaacccac cagttgacta taaagaagaa
    38941 gattactacc aacgtgaaga tgataagcct gagacagtaa aacgtcgttt ggatgttaat
    39001 attgctcaag gagaaccaat cattgctcac taccgtgcca aaggtttggt tcatgacatc
    39061 gaaggtaatc aagatatcaa tgatgtcttc tcagatattg aaaaagtatt gacaaatttg
    39121 aaataaagcg tttttcatac ttgcaaaaat tcgctataaa tgttatactg agatagtctg
    39181 acttataatt gttgtctctg tgtctagagg catcgaatcg aaatttatgg aggtgctttt
    39241 gcgtggcaaa agacgatgtg attgaagttg aaggcaaagt agttgataca atgccgaatg
    39301 caatgtttac ggttgaactt gaaaatggac atcagatttt agcaacagtt tctggtaaaa
    39361 ttcgtaaaaa ctatattcgt attttagcgg gagatcgtgt tactgtcgaa atgagtccat
    39421 atgacttgac acgtggacgt atcacttacc gctttaaata atcgaaaaac ttggagggat
    39481 aagaaatgaa agtaagacca tcggtcaaac caatttgcga atactgtaaa gttattcgtc
    39541 gtaatggtcg tgttatggta atttgcccag caaatccaaa acacaaacaa cgtcaaggat
    39601 aagatagaaa ggagaaaaca tggctcgtat tgctggagtt gatattccaa atgacaaacg
    39661 cgtagtaatc tcattgactt atgtttatgg tatcggactt gcaacatcta agaaaatttt
    39721 ggctgctgct ggaatctcag aagatgttcg tgtacgtgat cttacatcag atcaagaaga
    39781 tgctatccgt cgtgaagtgg atgcaatcaa agttgaaggt gaccttcgtc gtgaagtaaa
    39841 cttgaacatc aaacgtttga tggaaatcgg ttcataccgt ggtatccgtc accgtcgtgg
    39901 acttcctgtc cgtggacaaa atactaaaaa caacgctcgc actcgtaaag gtaaagctgt
    39961 tgcgattgct ggtaagaaaa aataatatag gaggtaaaag tcttggctaa accaacacgt
    40021 aaacgtcgtg tgaaaaagaa tatcgaatct ggtattgctc atattcacgc tacatttaat
    40081 aacactattg ttatgattac tgatgtgcat ggtaatgcaa ttgcttggtc atcagctggt
    40141 gctcttggtt tcaaaggttc tcgtaaatct acaccattcg ctgctcaaat ggcttctgaa
    40201 gctgctgcta aatctgcaca agaacacggt cttaaatcag ttgaagttac tgtaaaaggt
    40261 ccaggttctg gtcgtgagtc agctattcgt gcgcttgctg ccgctggtct tgaagtaaca
    40321 gcaattcgtg atgtgactcc agtgccacac aatggtgctc gtcctccaaa acgtcgccgt
    40381 gtataatcat cgcattacac tgcttttcgt ttaagaggga gtaactaaat gatcgagttt
    40441 gaaaaaccaa atataacaaa aattgatgaa aataaagatt atggcaagtt tgtaatcgaa
    40501 ccacttgaac gtggctacgg tacaactctt ggtaactctc ttcgtcgtgt acttctagct
    40561 tctctaccag gagcagctgt gacatctatc aacattgatg gtgtgttaca tgagtttgac
    40621 acagttccag gtgttcgtga agacgtgatg caaatcattc tgaacattaa aggaattgca
    40681 gtgaaatcgt acgttgaaga cgaaaaaatc atcgaactgg atgttgaagg tcctgctgaa
    40741 gtaacagctg gtgacatttt gacagatagc gatattgaaa ttgtaaatcc agatcattat
    40801 ctctttacaa tcggtgaagg ttcttctcta aaagcgacta tgactgttaa cagtggtcgt
    40861 ggatatgtac ctgctgatga aaataaaaag gataatgcac cagttggaac acttgctgta
    40921 gattctattt atacaccagt tacaaaagtc aactatcaag tggaacctgc tcgtgtaggt
    40981 agcaatgatg gtttcgacaa attaaccctt gaaatcttga caaatggaac aattattcca
    41041 gaagatgctt tagggctttc agcacgtatt ttgacagaac atcttgattt gtttacaaat
    41101 cttactgaga ttgctaagtc aactgaagtg atgaaagaag ctgatactga atctgacgac
    41161 cgtattttag atcgtacgat tgaggaactg gacttgtctg tgcgttcata caactgttta
    41221 aaacgtgccg gtatcaatac tgtgcatgat ttgacagaaa aatctgaagc agagatgatg
    41281 aaagtacgaa atcttggacg caagagtttg gaagaagtga aactcaaact cattgatttg
    41341 ggtcttggat taaaagataa ataaaggagg aatacatggc ttaccgtaaa ctaggacgca
    41401 ctagctcaca acgtaaagca atgcttcgcg atttgacaac tgaccttttg atcaacgaat
    41461 caatcgtgac aactgaagct cgtgctaaag aaatccgtaa aactgttgaa aaaatgatta
    41521 ctctaggtaa acgtggtgat ttgcatgcac gtcgtcaagc agctgctttc gtacgtaatg
    41581 aaatcgcatc tgaaaactat gatgaagcaa ctgataagta cacttctact acagcacttc
    41641 aaaaattgtt ctcagaaatc gcacctcgtt atgctgaacg taacggtgga tacactcgta
    41701 tccttaaaac tgaaccacgt cgtggtgatg cagcgccaat ggcgatcatc gaattagtat
    41761 aaaatcatca attttgttga gtgttatgat gatggagtct tgtgctctta gtctagctct
    41821 ggtctaccgc taggatttcg gtcctagcgg gaacactcat cataagttgg gatagtagac
    41881 gcttgtttac gaaattgttt ttttcttaag aacaacttcg taagcaggcg tttttgagta
    41941 ttttcgttag aattatgcta tactatttga aaagaatcct gtttaatgtt aaggtttctt
    42001 attttaagaa gaattggagt ttacttatga aagccattat aactgttgtt ggtaaagata
    42061 aatctggaat tgttgcaggt gtttctggta aaattgcaga attaggattg aatattgacg
    42121 atatctctca aactgtcttg gatgaatatt ttacgatgat ggctgttgta tctagtgatg
    42181 aaaagcaaga ttttacctat cttcgtaatg aatttgaagc ttttgggcaa actttgaatg
    42241 taaaaatcaa tattcagagt gcagcgattt tcgaagctat gtataatatc taggaggtca
    42301 tcatggatat tagacaagtt actgaaacca tcgccatgat tgaggagcaa aacttcgata
    42361 ttagaaccat taccatgggg atttctcttt tggactgtat cgatccagat atcaatcgtg
    42421 ctgcggagaa aatctatcaa aaaattacga caaaggcggc taatttagta gctgttggtg
    42481 atgaaattgc ggctgagttg ggaattccta tcgttaataa gcgtgtatcg gtgacaccta
    42541 tttctctgat tggggcagcg acagatgcga cggactacgt ggttctggca aaagcgcttg
    42601 ataaggctgc gaaagagatt ggtgtggact ttattggtgg tttttctgcc ttagtacaaa
    42661 aaggttatca aaagggagat gagattctca tcaattccat tcctcgcgct ttggctgaga
    42721 cggataaggt ctgctcgtca gtcaatatcg gctcaaccaa gtctggtatt aatatgacgg
    42781 ctgtggcaga tatgggacga attatcaagg aaacagcaaa tctttcagat atgggagtgg
    42841 ccaagttggt tgtattcgct aatgctgttg aggacaatcc atttatggcg ggtgcctttc
    42901 atggtgttgg ggaagcagat gttatcatca atgtcggagt ttctggtcct ggtgtggtga
    42961 aacgtgcttt ggaaaaagtt cgtggacaga gctttgatgt agtagccgaa acagttaaga
    43021 aaactgcctt taaaatcact cgtatcggtc aattggttgg tcaaatggcc agtgagagac
    43081 tgggtgtgga gtttggtatt gtggacttga gtttggcacc aacccctgcg gttggagact
    43141 ctgtggcacg tgtccttgag gaaatggggc tagaaacagt tggcacgcat ggaacgacgg
    43201 ctgccttggc cctcttgaac gaccaagtta aaaagggtgg agtgatggcc tgcaaccaag
    43261 tcggtggttt atctggcgcc tttatccctg tttctgagga tgaaggaatg attgctgcag
    43321 tgcaaaatgg ctctcttaat ttagaaaaac tagaagctat gacggctatc tgttctgttg
    43381 gattggatat gattgccatc ccagaagata cgcctgctga aaccattgcg gctatgattg
    43441 cggatgaagc agcaatcggt gttatcaaca tgaaaacaac agctgttcgt atcattccca
    43501 aaggaaaaga aggcgatatg attgagtttg gtggtctatt aggaactgca cccgttatga
    43561 aggttaatgg ggcttcgtct gtcgacttca tctctcgcgg tggacaaatc ccagcaccaa
    43621 ttcatagttt taaaaattaa gaaaatagga gaaattttaa gttctattta agattagacg
    43681 tgtatactat aatcattaaa taaagacctc ctaatattat ttgaaacaga taacactgaa
    43741 ttagtttgaa tttgattttc atctaatatc tttatttaat gaactcctaa actttttcat
    43801 aataatctcc ttcaaaagtc gcctgtatgg gtggctttta ttttatcatt catgatataa
    43861 tagaagcaaa cggaggacgg aaaatggtaa aagtacgatt gtatttggta cgtcatggca
    43921 agaccatgtt taacacgatt ggtcgcgcgc aaggttggag cgatactccc ttaactgctg
    43981 aaggtgaacg agggattcaa gagttaggaa tcggtttgcg agaatctgat ctacagtttg
    44041 agcgcgctta ttcgagtgat tctggtcgta ccattcagac catgggaatt atacttgaag
    44101 aacttggctt gcaggggaaa atcccttatc gcatggacaa gcgtatcaga gaatggtgtt
    44161 tcggtagttt tgatggagcc tatgatggcg atcttttcat gggcattatt cctcgtatct
    44221 ttaatgtgga ccacgttcac caattgtctt atgctgaact ggctgagggc ttggtagagg
    44281 tcgatacagc tggttgggct gaaggctggg aaaaactcag tggccgaatc aaggaaggct
    44341 ttgaaataat tgcaaaagaa atggaagatc aaggtggagg taacgccctt gttgtcagcc
    44401 atggaatgac tattggaacc attgtttatc tgattaatgg catgcatccg catggtctag
    44461 ataatggtag cgtgacgatt cttgaatatg aggacggtca gtttagggtt gaagttgtcg
    44521 gtgaccgtag ttaccgagag ctaggacgtg agaagatgga agaaggctct atttaatcag
    44581 tctagacttg cttgccatga gctagggatt tgataaggat atcaagataa gaaaaaacag
    44641 ccgagggtac tcctttcggc tgtttttgat gtggaaaact aaagtgtaat gctattgctt
    44701 ttagagattt tcataaacaa gagcaaggaa cctactgtta gaacagtcag gatagttgac
    44761 aaggctgcgg ctacaccgta atttcctctg agaacctctg tataaatagc tacagtcatt
    44821 gttcttgttt tgacattgta gaggaggata gaagtagaga gttttgaaat cattgtgacc
    44881 caagataaga tggctccaga aatgatacca gatagcatca ttggagttgt aatcttagca
    44941 aaggtattga gacgactact tcctaagctt tcagcagctt cttcaatact tggtgctatt
    45001 tgttgtaagc tagcaacaga tgagcgaata gtataaggta atcttctgac agatagagac
    45061 ataatcaaga tgaaagcagt ccctgtaatc ataagaaatc cacttccaaa tagaccagta
    45121 ttgaaggaag aaatgaaggc aatccctaga acggttcctg gtacaatata aggtaccata
    45181 ctgaggctgt caattaagtt tgtaaacaaa ttccgttttc taacggctag gtaggagata
    45241 aatgtcgcaa atagaacaac tagaactaag gcaatcaaag ggatacgaat ggtattgaaa
    45301 atagcagatc ccatacgatt gaaagctacc ttgtaactgt ttggagaata accttcaaca
    45361 aataccatac ctgatgtttt taggaaagag gtataaatta agtagatttg aggtaaaaca
    45421 gagataaaga taattccgta gactgttgca taaatggcag ccatttttcc ttttgtagtt
    45481 tttttaggct caattggatg gagcagattc atgctgaaac tgtagcggtt tgcaatgtgt
    45541 ttttggataa ggaaaattgc caaggcaatg ataatcgcca taattgcaaa agcagaattt
    45601 cctccaacct cgctaataaa ttgggtataa atcaggacag ggaaagtccg atatccttcg
    45661 ccaatcaaca taggcgttcc aaagtctgag aatgctctca taaatacaag caaggagctg
    45721 ctagtaaggt tggaactagg agaggtaaaa caaccgttac gatacgctta aatccgaagg
    45781 accccatgct ttcagctgct tcaagtagag aattgtcaat actgttcatt gttccagcaa
    45841 catatagaaa taccagtggg aatagttgca gtgtaaagac aagtacaatt cctttgaatc
    45901 cataaatatc gatagctgga agataaaggg catttgtcaa aaatttagtg atgacctcat
    45961 ttcgtcctag caagagaacc caggagtagg ctcctacgaa aggagctgac atggaagcaa
    46021 tgataatcaa tatttgtaga aatttctccc ccttgaagtc atacatagag aagagataag
    46081 ctaatagggt tcctacaact aaggaagtga cagtagcggt aatggaaacc ttgaaactgt
    46141 tgactagtgt ctcagagtag tagactttac taaagaaagt gacaaaatta gctagtgaga
    46201 attgtccttc atgtataagt gcttacttga gcacggtaac gataggataa acgagaaaga
    46261 caagataggt aagaaagatg aagaaagagg aggctgtcca aatatttagt tttttacgtt
    46321 ccatggttga ctccttttat caggttttgg gaaccatctg cagaaaagat gtttaatttt
    46381 tgcgtattga ttcgtagacg aatacgattg cctttttgta gatcttcttc aaaagttgat
    46441 tcttcactaa cttgaatttt tgaggcaaaa cctgtctcaa tgaaatactc cgtatttagt
    46501 ccaagataga cgctatcgct aatagttcct tcaatatctc cagattcatc tttgataaac
    46561 tcttcgggac gaatgcttac atgaatagct tgctcctcaa cctgatcaag agctagcatt
    46621 cgaagggcat agccatctga aaagacgata taagcgccgt cgctccgttt ttcaagattg
    46681 gcagggataa tatttgtgcg tccgataaag gttgccacaa actcattagc tggtttatga
    46741 tagagttctt ttggtcggcc gatttgttgg atcaccccat ctttcataac agcaatttgg
    46801 tctgaaatag ccatggcttc ttcttggtcg tgggttacat aaacagttgt aattcccact
    46861 tcgtgttgga tttctcggat ggcttgacgc atatccaagc gaagtttggc ctccagatta
    46921 ctaagtggct cgtccatgag gagaacactt agattaaccg ctaaggcgca tgccaaggtg
    46981 acacgttgtt gttgtccacc actgagttta tcgggctttc gatccgcata ttgagcaatt
    47041 tgcatgagtt caagatactt gttggtctgt tgaatcaatt cttcttttgg aaccttcttt
    47101 tgcataagac caaaagcaac gttgtctcgg acagtcaaat gtgggaaaat agcgtagttt
    47161 tggaaaacca tcccgatatt gcgtttgctg ggttccatat tattgatttt tgtatcatcg
    47221 aagtaaaatt ctccatcttt gatactgttg aaacctgcaa tcatacgaag aagggtcgtt
    47281 ttcccacatc ctgaagctcc aagaagggta aagagacttc cttttggaat tgtaatgttc
    47341 aaattctcaa taacagagac atcgtggtag atttttttgg cgttaataat ttcgatctca
    47401 ctcatagtga acctctttta ctgtttagat tggatatctg taaagacttc gttgtatttc
    47461 ttaacgatat ctgatttatt cttgatgaca taatcataat cttcagtgag tgttttgatt
    47521 ttgtcaattg gtttcatgtt ttcgcttgtt ttagcatttt tacgaacagg acggttagta
    47581 gtggttgtac caagtgtatc ttgtacttct tgagagataa taaaatcgat aaatttcttg
    47641 gcattttcca tatttttaga ttttttaacg atagcagcac tagcaggtag gaagacggtt
    47701 ccttcttttg gatagactac cttaatgtta gctccgtcat ttaagagttt aactgctgga
    47761 tcttcataag agagaccaac agtcatttct ccatcagcga ctgctttata gacaccagat
    47821 gaacttaaac cgattttata gtatattgaa actagaatag tacacatcta cttctaaaac
    47881 attgttagaa atcgatttga ctgtcctgat taatttgtcc tgttcttatt tcattttact
    47941 ataccatcaa taagtgtgaa aagatctttt acataagacc aagccttatc atctttgtaa
    48001 ccaccttgag cttgtagcat atttgttaat tgagcaaagg cgctagaaga gtttgctggg
    48061 tcagcagttg cgatttttcc ttttagttca ggtttgagaa ggtcgctata tccttcgatg
    48121 ttcatgcctt tagttaaatc agggtggacg attaaaacac taccatctag tgtataagga
    48181 gtagagaagc cagttgtgtt ttgatattct ttgataacat tatcattttc ttttgaaata
    48241 tagttttcaa agagttctcc gtgggtagca tattgtgtat aagaaccacc aaagataaca
    48301 tcagctacag gaacttcttt ttctgactct agttttttga aaagttctcc agtaccagct
    48361 tgaatcagtt ctattttgat accatatttt tcttcaaagg caggaatagt tgctccaatt
    48421 aagccttctg agtttggtca ataaacgact agcgaaccac cgtctccttt atcagatgaa
    48481 ctgtcatcgg cagattcatt agaagaacaa gcagcataat acatccattt ctttttcatg
    48541 atggatacct ccgttgtgtt atttaagttt attttaaaac aatgtaagcg tttttaaaac
    48601 ctacaattct attctatagt gtattgaatc tataacagta cactttgact gctaaaatat
    48661 ttctataaat taatttgact ttcctgatag agatgttcac atcttatttc aattcactat
    48721 attagagtaa aattctctac aaaaagaaga atagcctatt ttactattct tccgagtgat
    48781 ttcaatttct ttggggaaat atggagatac tttttaaata ctttggcaaa atatttatag
    48841 tctgaaaatc caacttggtc agagatagcg cttaaagggg agttgggaga ttcagccatc
    48901 ttatctatag cttgtttcag gcgatattgt aagatatatt catttagagt catggggaag
    48961 tcttctttga taaccttata tagatgcttg ttatctgcaa atagctagag cgtttcaagg
    49021 aaatgaacta gaaataacaa ttaatatggt ggttcagaaa taccttttga taatcaataa
    49081 gatcttcagg atgaagtgcg ttgactccat ccatttcata tttccgattt aataaacgat
    49141 atttgttttc accaaattga tgaaaaggga gtagttgaac ttggtcgata tttaatgagt
    49201 taaatagagt agcgaatttt tctgcatcct ctaaactatt gttaaaatta ggaataactg
    49261 ggattcttaa aacgatagtt ttattttgag aaaaagcata atgaatgttt ttaataatca
    49321 tttgattaaa aaccccagtc acttttttat gttttataga attataatgt tttaggtctg
    49381 tgtagataaa atccacatat tgaattaaat caataaattt ttcatgatca acaaaggcag
    49441 tagtttcaat ggcagtgtgt atgtgatgtt ctttagctga ttttaagatg gctttagcaa
    49501 attcaaactg agcaaatatt tcacctcctg ataaagttaa acctccaccg gattcttcgt
    49561 aaaattcttt gtcttttaat acctctgtaa taatttcttc tacagtcttt tcttcaccga
    49621 ctaaggtgaa tttctctcgt tgagcatctt tcattttttc aggtttcatt ctttgagatt
    49681 caggattaga acaccatgga cagcgcagag gacatccttt taaaaaaaca gttgtacgaa
    49741 tacccggacc gtcatgaatt gaaaagtgtt gaatattaaa aataattcct ttagatattt
    49801 ccataagagt tcctcctctt cataagtcta tcctatcaca aaaacgaaag aaaaacaatt
    49861 acgaacgaaa gtttaatttg ttgttttttg ctttttattt tggtagaata aaacggaggt
    49921 gtaaaatgaa aagattggaa caaattatta aattagtatc agaacatgaa aagatcgacg
    49981 ttaatacatt atcggaaaaa ttaaatgtat cgaaagtaac gattagaaaa gatttagata
    50041 aattagagtc aaaaggttta ttacacagag agcatggata tgctgtatta aatagtggag
    50101 atgacttaaa tgtacgtttg tcaattaact atgaaattaa gagaaaaatt gttcaggaag
    50161 cagtaaaatt ggtgtcagat aatgaaacaa taatgataga atctggatcg acctgtgctt
    50221 tacttgctga ggaaatttgc aagcaaaaaa gaaatgttac gattgtaaca aattcatttt
    50281 ttatagcaaa ttttgtgaga gcttatgatt catgtcgtat tattgttctt ggtggagagt
    50341 ttcagaagga ttcacaggtt actgtgggac ctttattaaa agaaatgata cagacttttc
    50401 atgtgcatca agcttttgtt gggacagatg gttacgataa agagatgggc tttacaggaa
    50461 aagatttaat gcgcagtgag gtagttcaat atatttcagc agcgtcggat aaagtcattg
    50521 tactaactga ctcaagtaaa tttgataaaa gaggtacagt aagaagattt gctttaagtc
    50581 aagtctatga agtaataaca gacgaaaaac tttctaaaca aaatatagct acattagaaa
    50641 atgctgggat aatggttaag gtagtttcgt aagaggttaa gtgtatgaat caagatagga
    50701 ataaactgct ttctaaaatt gcttatctgt attatattga aaacttaaat cagtcacaaa
    50761 tagcagcaaa attaggaatt tat
//
LOCUS       DAVKWZ010000008        46418 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000008 DAVKWZ010000000
VERSION     DAVKWZ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 46418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46418
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.008"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(464..2191)
                     /gene="ezrA"
                     /locus_tag="VW854_000522"
     CDS             complement(464..2191)
                     /gene="ezrA"
                     /locus_tag="VW854_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEV0261809.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2273..4219)
                     /gene="gyrB"
                     /locus_tag="VW854_000523"
     CDS             complement(2273..4219)
                     /gene="gyrB"
                     /locus_tag="VW854_000523"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEV0261810.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFIPDPKIFTETTTFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4234..4797)
                     /locus_tag="VW854_000524"
     CDS             complement(4234..4797)
                     /locus_tag="VW854_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV0261811.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEVISNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4882..5436)
                     /locus_tag="VW854_000525"
     CDS             complement(4882..5436)
                     /locus_tag="VW854_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEV0261812.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5447..6670)
                     /locus_tag="VW854_000526"
     CDS             complement(5447..6670)
                     /locus_tag="VW854_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV0261813.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6707..9157)
                     /locus_tag="VW854_000527"
     CDS             complement(6707..9157)
                     /locus_tag="VW854_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEV0261814.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLRKKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTEASLEPRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEDFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPSVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9357..10286)
                     /locus_tag="VW854_000528"
     CDS             complement(9357..10286)
                     /locus_tag="VW854_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV0261815.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELIVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARSLIQEGNFSH
                     AQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVR"
     gene            10426..10713
                     /locus_tag="VW854_000529"
     CDS             10426..10713
                     /locus_tag="VW854_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEV0261816.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10747..12081)
                     /gene="ciaH"
                     /locus_tag="VW854_000530"
     CDS             complement(10747..12081)
                     /gene="ciaH"
                     /locus_tag="VW854_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEV0261817.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(12071..12745)
                     /gene="ciaR"
                     /locus_tag="VW854_000531"
     CDS             complement(12071..12745)
                     /gene="ciaR"
                     /locus_tag="VW854_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEV0261818.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(12854..15400)
                     /locus_tag="VW854_000532"
     CDS             complement(12854..15400)
                     /locus_tag="VW854_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEV0261819.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(15523..15963)
                     /locus_tag="VW854_000533"
     CDS             complement(15523..15963)
                     /locus_tag="VW854_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEV0261820.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQAVCVVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTSESKVVLEEFRKVYPL"
     gene            complement(15938..16807)
                     /locus_tag="VW854_000534"
     CDS             complement(15938..16807)
                     /locus_tag="VW854_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEV0261821.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLDTICSFYLDKEVWYRFSELTNTEANCLAGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAESHVVTEVYQTNPNLSLIFPFVNDADQLKQAITVLRQYGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKINFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(16849..17577)
                     /locus_tag="VW854_000535"
     CDS             complement(16849..17577)
                     /locus_tag="VW854_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0261822.1"
                     /translation="MELFKTWKKNMVLYGLKSQIGTVYRNNDRTTSFYDVGNFLYLAG
                     ELDSRFWEDFVRKYGLDYKIIISENTNWQDFLHRKVGLNSFTRYSFKDKANFQVEFLN
                     NLVTHLEEGYNIVPIDNHIYNCFSTEEWSQDLQGDFESYQDFVLKGGFGFVILKNNEL
                     IAGISSGLVYRKAVEVEVATRPNEQGNGFAKKLGAAMILESLNRDMFPLWDAHNEASK
                     KVAEFLGYELSEPYEAFELEEILI"
     gene            complement(17585..18064)
                     /locus_tag="VW854_000536"
     CDS             complement(17585..18064)
                     /locus_tag="VW854_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV0261823.1"
                     /translation="MEIKKHFGVYAVCLENGKLLCIEKARGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELTNFMVHHVMALYDVEMNESAPQV
                     TTSEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVK"
     gene            complement(18075..18773)
                     /locus_tag="VW854_000537"
     CDS             complement(18075..18773)
                     /locus_tag="VW854_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEV0261824.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(18766..19032)
                     /locus_tag="VW854_000538"
     CDS             complement(18766..19032)
                     /locus_tag="VW854_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEV0261825.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTACDVLATDWVEVHD"
     gene            complement(19100..20026)
                     /locus_tag="VW854_000539"
     CDS             complement(19100..20026)
                     /locus_tag="VW854_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEV0261826.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFNLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSQAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(20101..20916)
                     /locus_tag="VW854_000540"
     CDS             complement(20101..20916)
                     /locus_tag="VW854_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261827.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(20920..21240)
                     /locus_tag="VW854_000541"
     CDS             complement(20920..21240)
                     /locus_tag="VW854_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV0261828.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            complement(21447..21527)
                     /locus_tag="VW854_000542"
                     /pseudo
     CDS             complement(21447..21527)
                     /locus_tag="VW854_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(21571..23568)
                     /gene="metG"
                     /locus_tag="VW854_000543"
     CDS             complement(21571..23568)
                     /gene="metG"
                     /locus_tag="VW854_000543"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEV0261829.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQVYVDRMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWILAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIIAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            complement(23692..24951)
                     /locus_tag="VW854_000544"
     CDS             complement(23692..24951)
                     /locus_tag="VW854_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0261830.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNVDEIANIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(24953..25654)
                     /locus_tag="VW854_000545"
     CDS             complement(24953..25654)
                     /locus_tag="VW854_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0261831.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAVYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(25638..26837)
                     /locus_tag="VW854_000546"
     CDS             complement(25638..26837)
                     /locus_tag="VW854_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV0261832.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTAVKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAQVPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            27123..28469
                     /gene="gor"
                     /locus_tag="VW854_000547"
     CDS             27123..28469
                     /gene="gor"
                     /locus_tag="VW854_000547"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEV0261833.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESVAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RINLPLHTHKVPAKLEKTAKGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
     gene            28666..29202
                     /locus_tag="VW854_000548"
     CDS             28666..29202
                     /locus_tag="VW854_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEV0261834.1"
                     /translation="MKKAHVYAIPAIGAALIAVLAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALVVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            29257..29337
                     /locus_tag="VW854_000549"
                     /pseudo
     CDS             29257..29337
                     /locus_tag="VW854_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(29406..29621)
                     /locus_tag="VW854_000550"
     CDS             complement(29406..29621)
                     /locus_tag="VW854_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEV0261835.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            29719..30039
                     /locus_tag="VW854_000551"
     CDS             29719..30039
                     /locus_tag="VW854_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261836.1"
                     /translation="MRHKFQQVLNKIHDFLNGYDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFSKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(30069..30407)
                     /locus_tag="VW854_000552"
     CDS             complement(30069..30407)
                     /locus_tag="VW854_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261837.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(30419..31138)
                     /gene="trmD"
                     /locus_tag="VW854_000553"
     CDS             complement(30419..31138)
                     /gene="trmD"
                     /locus_tag="VW854_000553"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEV0261838.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRVQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(31128..31646)
                     /gene="rimM"
                     /locus_tag="VW854_000554"
     CDS             complement(31128..31646)
                     /gene="rimM"
                     /locus_tag="VW854_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEV0261839.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            complement(31677..32465)
                     /locus_tag="VW854_000555"
     CDS             complement(31677..32465)
                     /locus_tag="VW854_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV0261840.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADVVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKCQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDEYGPELYLKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFEDVSIEAHIPVFVFCQP
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELSE"
     gene            complement(32569..32812)
                     /locus_tag="VW854_000556"
                     /pseudo
     CDS             complement(32569..32812)
                     /locus_tag="VW854_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
     gene            complement(33042..33281)
                     /locus_tag="VW854_000557"
     CDS             complement(33042..33281)
                     /locus_tag="VW854_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEV0261841.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            complement(33301..33573)
                     /gene="rpsP"
                     /locus_tag="VW854_000558"
     CDS             complement(33301..33573)
                     /gene="rpsP"
                     /locus_tag="VW854_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEV0261842.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            33768..33968
                     /locus_tag="VW854_000559"
     CDS             33768..33968
                     /locus_tag="VW854_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261843.1"
                     /translation="MNTILEKFYKEHQVKPFISPERDLDTWLLSPKPVPKRNMNLLAD
                     DSLAGDIILLWRIQFGTFTTET"
     gene            34447..34659
                     /locus_tag="VW854_000560"
     CDS             34447..34659
                     /locus_tag="VW854_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261844.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEDFQDDDEIPTII"
     gene            34743..35546
                     /locus_tag="VW854_000561"
     CDS             34743..35546
                     /locus_tag="VW854_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV0261845.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKKGGNPSLD
                     GKHPVFGQVIGGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(35602..37143)
                     /locus_tag="VW854_000562"
     CDS             complement(35602..37143)
                     /locus_tag="VW854_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV0261846.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKILLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(37145..37627)
                     /locus_tag="VW854_000563"
     CDS             complement(37145..37627)
                     /locus_tag="VW854_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV0261847.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVCFCFTPQEQSTVGVGTPGIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            complement(37627..38712)
                     /gene="rlmN"
                     /locus_tag="VW854_000564"
     CDS             complement(37627..38712)
                     /gene="rlmN"
                     /locus_tag="VW854_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEV0261848.1"
                     /translation="MKPSIHSLAHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMCQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERVNHIVVM
                     GIGEPFDNYNNVLNFFRTINDDKGMAIGARHITVSTSGLAHKIRDFADEGVQINLAVS
                     LHALNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALE
                     LAELLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGGNCVVRQEHGT
                     DIDAACGQLRFNTMKRDRQKAVAAVNP"
     gene            complement(38733..39263)
                     /locus_tag="VW854_000565"
     CDS             complement(38733..39263)
                     /locus_tag="VW854_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEV0261849.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFALVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKEQRHFVIRQK"
     gene            complement(39420..40025)
                     /locus_tag="VW854_000566"
     CDS             complement(39420..40025)
                     /locus_tag="VW854_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEV0261850.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            complement(40198..41235)
                     /locus_tag="VW854_000567"
     CDS             complement(40198..41235)
                     /locus_tag="VW854_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV0261851.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQMLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKANSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            complement(41269..42204)
                     /locus_tag="VW854_000568"
     CDS             complement(41269..42204)
                     /locus_tag="VW854_000568"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEV0261852.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDCITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            complement(42757..43947)
                     /locus_tag="VW854_000569"
     CDS             complement(42757..43947)
                     /locus_tag="VW854_000569"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEV0261853.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAADLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRTAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            complement(44162..45505)
                     /locus_tag="VW854_000570"
     CDS             complement(44162..45505)
                     /locus_tag="VW854_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV0261854.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            complement(45885..46397)
                     /locus_tag="VW854_000571"
     CDS             complement(45885..46397)
                     /locus_tag="VW854_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001852542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV0261855.1"
                     /translation="MPAEVLSLLPQSGTYTTSSSVAIAFVPEAPMPVEVAVANGTWTF
                     LGYQEVEEGSNLTFVGKWVFEAKQDPSPQPQPAPTPQPAPQPNPVPPVNPGGEQGTDN
                     NQADRHQSEQPRKPEENPPAQGPAGEKEKQATLPNAGSTAPLSLTGLVTSSLLAGLAV
                     LLLGRKRKDD"
BASE COUNT        13770 a        10145 c         8347 g        14156 t
ORIGIN      
        1 ctagaatgtc ttccaaacga gaaaactctc gtaaacaaag aggttttaga ggcctattta
       61 ccgtggacta aagttgtaca agaaaagtgt aaataagaaa tctccagatt aggaactatc
      121 cgtgagttct ctagtctgga gatttttcaa tagacttcgt tattggacgg ttaccaagtt
      181 actcaaagta cagatttgaa gctgcagata agactgacga aatcacttta atactaaatg
      241 aaaatcaaaa agcaaactaa gagcctcagg ctgtacttga gtacggtaag gcgacgctga
      301 cgtgatttga agagattttc gaagagtata aaacactgtt ttgaggtggt ggataaatcg
      361 gctgggcaaa aactcaatct caggagaaaa tgaagtaaat ctccccacaa ctatagaaaa
      421 agatttgatt ctgctcctca cacaataaaa tctttttctt ttattaaaaa cgaatcgttt
      481 cacgtgtttt ctcatatgag gtaacaaagc gattggttac accaggctct gccacttcca
      541 atgcttgaga aatcttgtca aatgaagcgt gataatcaaa ttctttttca aaaatatcta
      601 aagcttcgtt aaatgcttct tgaatgcgtt catcaaatga gcgatagcgg ttagaatatt
      661 gtaagagttg ctctgtcaaa gttgcatatt gtacaatatt ataagtttcc gtttctaaag
      721 cttccatatc attcgttgca atttcaagaa ctcgggtaac agattcaatg ttaatcattt
      781 tttgttctaa ctcaaccatt aaatcctcgg tattattgct tgccgtaaag aataacttca
      841 agaaagtttg tggaatacct ggcagattgc gtttttccat gtatcgcttg atagtatgga
      901 gacgattgac ataaacattg gccttttgac gtgcattaat atcatctttc tcaatttgtg
      961 tcaggcgctc actaactgaa atttgctcat cttcaatatc ttttagttga gtttgtaaat
     1021 cctcaagatt ttcttcaaga actgaataag cttgggttgg ttcttcttga tttgaagtta
     1081 cctcaacaat agctgcctca aaactctcta attctgtctg aatacgacga acatggcttg
     1141 cagctgtctc aggaagtaaa taggtcttgt tcaaacgtgc aatatcttcc cccaataaag
     1201 tattattctc tttcatatgt tgaagataag ttggaagagt tgcaagtaga ttttccacta
     1261 ctttctgagc agcaatttct cgagtaaaaa tatcatacaa ggcattgatt tcctcttgtg
     1321 cctgtccatt ctcatattcg gcattatcca attccaactg acgaatattc tcttggtttt
     1381 tcttgaatgc ttcataaagc aagtggaaac gcgcttcaat atccgtttca acaaaatgat
     1441 aattagcatc aattagttta cgataaccgg cttccaaatc ctgtaattga tctggcaatt
     1501 ctgtagaaag cgtcgtaacc aaggctggaa cacgatccac aatatgactt aaggccaaaa
     1561 tgtgattttc tgtattatcc aaaatcactg cggcttccac agggtcaccc gatgaattca
     1621 aggttacaaa ttgtgaaaat tcagattgga tattttctaa ttgtttttca atttcatcca
     1681 aggcttgacc atactgttct gaattttcag caactctatg ctgaagttcc tcaaataaat
     1741 ccaaagcatg aagaacacga ccactatttt tagattcttg cttctctaag tctgccaaag
     1801 cattgcgaat tgccgcaata tcttcttcaa tcaaagtaat ttgactctca atttggtcaa
     1861 tttgatgact ggccttgaga aaacgaaatg aatggttata gccttctgct tcaaagagat
     1921 tattttcaat atcggcaaaa gagttgagag ataaatcgac ccatttttga ttccattcac
     1981 ggaaagccac ttgactttgt ccaatcaagt gcatattttt tacagcttct acttcatcat
     2041 ttactggaag attgtatagt tcttcttttc tttcttctag cgcctctaat ctcccctcgt
     2101 ttcgcttacg tagaaagatt gccactacat aagccagaac taaaatgact gcaattgcaa
     2161 ccattaaata aattagttgt ccattagaca tatcaaactc cttttttact tgaaacaatc
     2221 gtaacgatta tatcatattt tttagaccaa gggaactact tctccctatt ttttagacat
     2281 caagtgtact atagacagca ttttcttcga taaactcacg acgaggctct actcgatccc
     2341 ccatcaacat atcaaagatt ttatctgctt ctgcagcatc atctacagaa actctagcca
     2401 tcaagcgatg ttcgggatcc atggttgttt cccacagctg atgatcgtcc atttcaccta
     2461 gccccttata acgctgaata gtcggtttgg tacgaccttc actataacgg gctaaagctt
     2521 cttggagttt gatttcttga tctgcacccg gctggatata ttctttaatc tcgcttccaa
     2581 ccttgacacc atagattggt ggttgggcaa tataaacata accagcttct aggattggtt
     2641 tcatataacg ataaatcaag gttaaaagaa gggtacgaat gtgggctcca tcgacatcgg
     2701 catcggtcat caaaacgagt ttttggtaac gggctttcga aacatcaaat tctgcgccaa
     2761 atcctgttcc catggctgtg aaaagactac gaatttcttc gttagctaga atcttatcca
     2821 tacttgcttt ttcaacgttc aaaatcttac cgcgaattgg aaggatagcc tgaaactcac
     2881 ggttacgacc agatttggct gatccaccag ctgagtctcc ttcgacgatg aagagttctg
     2941 tttcagcagg gttattagaa gaacagtctg ctagtttccc tggaaggttg gaaatttcca
     3001 aaccagattt tttacgtgtg acttcacgcg cacgcttggc agccacacga gccttggcag
     3061 ctaaaatccc tttttccacg atacgtttgg caatctgtgg attttccatg aggaaatcgg
     3121 agaaggcttc actgaagagg cgattggtaa tcttgaccac ttcgctattt cccaatttgg
     3181 tcttggtttg tccttcaaac tgtggatttg ggtgtttaac tgagataact gcagttaagc
     3241 cttcgcgaac atcttcccct gttaaattat cttcattgtc tttcagtaac ttattcttac
     3301 gagcataatc attgataaca cgagtcagtg ccgtacggaa accttgttca tgcgttccac
     3361 cttcatgggt atgaatatta ttggcgaaac tcatgacatt ttcatggtaa cctgttgtgt
     3421 actgcatggc tacctcaact gtaatatcat ccatctcacc gtctgtatag attggtgtat
     3481 caaagattac atccttgttc tcgttgatat attcaacgta actagcaatc ccaccttcat
     3541 aatgataatg cttggtttgt tccaaacctt ggcgcttatc agtgatagaa atttgaagac
     3601 cgcgatttag aaaggccaac tcttgaatcc gtttatttaa tttatcaaaa tcaaaggttg
     3661 ttgtttcagt aaagattttt gggtccggta tgaagtgaac cgttgttcca gttctatctg
     3721 tatccccaac cacctcaagg tctgcaacaa catgaccacg acggtattct tggtaatgaa
     3781 tcttaccgtt tttatggaca tgaacgtcta attgagtgga aagggcatta actactgacg
     3841 accccacccc gtgaagacca cctgaaacct tgtatccacc accgccgaac tttcctccag
     3901 catgaaggac tgtaaagacg gtctcaacag caggacggcc tgttttttcc tgaatatcga
     3961 ctgggatacc acgcccatca tccacaacag taatcgaatc atctggctca ataaaaactt
     4021 gaatatggct ggcaaatcct gccaaggcct cgtcaattga gttatcaaca atttcccaga
     4081 ctagatggtg aagaccttct tttgaggttg atccaatgta catccctgga cgcatacgaa
     4141 cagcctctaa gccctctaaa acttgaattt gactggcatc ataatcctgt gcctgcagat
     4201 ttttgatttc ttctgtcatc ttattccttt ttcttatatg tctaatactt gtcttaaatt
     4261 cacgatactg gtaaacaagt gggtatcaag tcctgcagct tgacctgctt cgatatcaat
     4321 cggccgatca ccaatgacaa gaccagagct aatctgatac ttttctctta aataaagcat
     4381 ggattcggga tttggctttc tcttaaagcc tgagctagaa gtcaccactt ctgtaaaata
     4441 agctgctata gaggtttttt ctaaaatttc caaaacctga tcatttcgat gagagaccaa
     4501 aaaatgacgg ccaccttgat ttgaaatgac ttccaatagg tcagaaactc cttcaaataa
     4561 aatcgggtgt tcaagctctc tggcttcatt ttccttgtac ttttctaaaa aattctctaa
     4621 attgggagcg aatgtctcaa tcgcaaaagg agtagaaacc tttaaagctt gatagacact
     4681 gtcatggtct tgtgtgatac catacagtgc caatgtttca acaaatgcag ctgttgaagt
     4741 ttcataatta tccagtaaag ttccacctaa atcccagatg taatcgtgat atttcatacc
     4801 cttcattata gcattttttt atgaaaaaag cgatgatttc cctgagtttt ccacataaaa
     4861 aacgagagaa taactcccgt tttactgatt tttaccaaag acatctcgat agagcattcc
     4921 tgttcgtaaa ctctctgcgt cccctcctag ttgctccact aactcgtagg caaaggcaag
     4981 ggctgttgaa ggaccccgac tggttgtcaa ctgaccatct accactactg tttccttgac
     5041 gtagtgacca tcaaggattt gctcttgaac gccgtcataa caagtgtatc gcttattttt
     5101 caatatctct gcttgattga gggcaattgg tgccgcacaa atggctgcta gtttcttccc
     5161 ttcttgctcg aagctttgca attcttgaat caaggtctga ttatcacgta aatgtgcaga
     5221 accaggcata cctccaggaa gaacaatcat atcatagtct gataaatctc catcaaagac
     5281 atgatctgct cttacttgga ttgcatgcga acccgttact tgctcttcaa aaccaaccat
     5341 atcacatgtg atattggctc gacgcaagac atctacaact gtcaaggctt caatttcttc
     5401 aaagccctga gctaacataa ctgctacttt taccatgatt ttctccttat ttaatttgtt
     5461 ttaatacaac ctttttccgt ttgaatggga cttttccgct cttttcttca gctagattag
     5521 tctggtaact catcgataaa agcaaaccaa caccaatcag attactgata atagctgatc
     5581 ccccttgcga aatgaaaggc aagggaatcc ccgtcaaagg aagtagtcca gtcacagcac
     5641 cgatattctc aaagatgtgg aagagcaaca tcataatcaa acctgtggaa atataagtgt
     5701 agaactggtt atttgattta agagtaatct tcaacatacg gtaaatcaac atgagataga
     5761 gggcaataac caggacagag ccaataaagc caaaatcttc tgcaataacc gtaaaaatca
     5821 tatctgactc tcgaactggg ataagcagat tcgaagcatt aaatccctga ccaaataagc
     5881 caccactccc aatggcaatc tgcccttgag cctgctggta agtcgttgtt tgggcaaact
     5941 caaagggatt gagccaagcc aaaatccgat taatttggta ggtcggcatt ccaatctggt
     6001 gaagaaaagc tcgtccgtcc ttgctaataa agatagctaa gaaaccagca actcctgtta
     6061 cagcagtcac aaatactggg ataataattt tccaagaaac ccctgataat aaaacgattc
     6121 ctgagaaaat ggctacaaaa accaaagccg tccccaagtc actttgaagt gctaaaagaa
     6181 ctaggactgg aatggtaaag agaatcatcc agaaaattaa caaaaagtcc agcggaaccg
     6241 tgcgtctcca ttccttatgt ttctttgtaa attggacaat gacacgagcc aacatgagga
     6301 tataggatat cttcataaat tctgacggtt ggaatagggt aattccattt attgataccc
     6361 agtttttggc acccgttgat gcaactaagc ttggattata aaatacaatc ggcaagatca
     6421 taagtcccaa gcctaaaata tatagaaagg gggtcacctt ccaaagaaat tctgtattaa
     6481 agagcatgac cacaaaacca atcacaagcc ccaaggcaat ccaggcgacc tgctgcccta
     6541 aaatgggcag aatattattg ggataatcat gactaacggc tatatagata gccaccacac
     6601 cgatgaccag tagaaaaaat actggcaaga gcaaactata atcgactcta gagtcgagag
     6661 aacgtttcat atatactaac cttatacttt catacaatac tatttatcaa agctcattaa
     6721 aaaatctatc aatagcctcg tcaacttcgg atcgagagat ggttttaaca gtcgcttctt
     6781 ctgctagaga tgctactatt tgtttgccgt atcgttttcc gacgattctc ctatccaaaa
     6841 taagagttaa ggaacgttgg tattcacgtc tcatacttct tcccaaagcc tgttttaaac
     6901 gaataatggc cattggcaat tgataatcat aaaaggcatt tttcccttct tgattcagtt
     6961 cttgattaat ctttttcgtc aagggttctt gaggattttg gaaaggaagc ctcggtacaa
     7021 cttgaatcac agaaggatgg cttgaaaaat caactccctc ccagaaactt gctgcaccaa
     7081 gcaagatttg ttgttcacct ttttcaaagc gtttctttag ctgatgaaca tccccatttt
     7141 tatactgggc caagtggcta actgtaagta aatccgatac tgctagaagc atgtctttag
     7201 cggtaaagag aaccaaaatc ggttgctgga aagcttgaat ttccactagt aaagcagcta
     7261 cctctctggc atagacttct aaggaggttt ctgttaccag agggaaatct ttgaccatga
     7321 ccacttcttg ttcctgtttt ccccgagatt caatcttgac aaatttagct tcaggatagc
     7381 ctaaaaggtc tgccaaagaa accctctgac taatctcaag agtagccgat actcccaaga
     7441 cttggcaact ctcaggaagt aaagaggaca gaagagtacg gcctgatttt gtagaagaaa
     7501 tctgaatttt cttttgactc gtttcagtta cttcaagcca gtaatcacct tcagctgtaa
     7561 aatagcgaac cagctcatca aagtcttcta cttccaattc tgaaaaatac tgatggagat
     7621 tgtccagaga atctaagata tttttcctag atttgccaga ctgaaattgt tctatcaagt
     7681 agagacactc aaagcgaata ctttctagta tccgttgttg aaccctgttt tcttctccta
     7741 ctaaagcctt atcaattaaa tcgataatag attgtatatc gtaggtctct tgaagcagat
     7801 tttctagagc taacaaaatc ttttggactt catcaataat cagtaaacgg tcactgacaa
     7861 attcaggatt atcttcaagt ctggttacga gataggcatg attagtcact aaaagcttgc
     7921 aagtctctgc cctttcttga ctacgtttcc aaaaatcttc cgtcacaaat aagctctggg
     7981 atgataaatt cccatcatga cgaaggtctg ctagaaaatg ttggtaacgg tagagttgcc
     8041 cgatttcatc caaatctcct gtctctgtct cagtaagcca gaccaagact tgcattttaa
     8101 agcgtctaaa taagcgattt tcatcatttt cctgcaagga atgataaaag gcatccaact
     8161 tcagataatt ttgtggtccc tttaagctat gaatatctgt atggaacact tccttgaggc
     8221 gtttaccttc ttcttccatg atttgatttt gaagaatctt tgtcggaaca ctaagaacaa
     8281 tttgtcgctc tttggattga gataaagcgg gtaagagata gccataggtt ttcccaatcc
     8341 ctgtcggcgc ttgaatcaga gagacaggtt catctttcaa tagcaagcca acctctttag
     8401 caaaactttc ttgttcctcc ctcacttcaa ggttcaacag agaaatattt ttagaaaagt
     8461 cttgagatag ttttcgtggc tccagagaag cttccgtttt cttaaaatat agaccttgaa
     8521 cttggaccaa gtctggagaa ctcaggatag attggttgcg ataagtttcc tcaataacca
     8581 ggtaggactc atataggaga gcgtcagcca tttccagcaa gcgttccaag agacctttag
     8641 gaagctgggt catctttttc cgtaaaaaaa gaagtaattc tgctgtagct tgggcatctg
     8701 aaagggctgt gtgtgcgtgt ttaagaggaa ttcctaattc tcgacacaaa atcggcaaac
     8761 tatatttttc cagttcaggg aaaaagacct gggccaattc gaccgtatca acacgagggt
     8821 ttcttagctc atagccttca aaaaataaat tttccgccaa gagattagca tcaaactgaa
     8881 cattatgggc tacaaaaatc ccatcctcca ccaagtcaaa tatttttctg gcaacttgcg
     8941 aaaaatcagg tgcttgcgcc agacgttggt ctgtcaatcc tgtcagttct ttgatatgag
     9001 catccaaggg ttcatgtgga ttgacatccg tcgtatagtg atcgacgatt tctccgtcct
     9061 caatcacgac aattcccact tggataattt tagccttact acctgtgcta gttgcctcta
     9121 aatccaccac aacatagcga ttaccaatcg ttttcattat aaaaaattat accaaaaaaa
     9181 gggccatttg gcacctgcaa acataggata attttcaaac tcaagaacac tacttttctt
     9241 aaaaatccca tcgaatagag cattctagcc tgctgatttt tgtaaaatag tgaagaaata
     9301 cgaaaaattg agagaagaaa tgtttgcatc ttccttctct caacgaatca tgtattttat
     9361 ctaaccatgt caggatcgta gtcataaaat cctgtatcaa ggaaacggtt tttagggtga
     9421 agtttacgaa cttcttcatc cagcaagaag gcttgagcat gactaaagtt gccctcttga
     9481 atcaagctac gcgcttggat aaagagtttt gcaatctcaa caaagttctg accaggagtg
     9541 tagagccctt ctagtttcca gcgagtaaag ccatgctcca ccaattctgt taatttgatc
     9601 atcaaatcaa ggtcattgtt ggcaaagata tgggtcccat gattatcttc aaaaatggaa
     9661 tagtggctct ctggatcact tggctcagcc aagaagaggt cacgtttatg cgtcttttca
     9721 tcatcgatat gtgtaaagtt atagtagttt tgcaagagtg gacgtttaga atgatggatg
     9781 acgctagcac cgtaaaccaa aacttcagca ggaatttcca aaatctctgg cattttgaaa
     9841 agttcagctg atggaatttc acgcgccaaa acagcctcag atgcgccagc cttttgtccc
     9901 cagaagttaa tctgacgact gctagttacc atggttgaag catcgtagat ggtcttaaat
     9961 gaataaccat cgcggttaac tacgtaaaag acgcctgcat ccccaatcgt aatatagtct
    10021 gtcttgattt cttccaagaa gtctaagaaa ggcttgatac ggtccatcat atcttggtgc
    10081 atgagagcat tgaccgcaac gatcaattcc ttaccagcat catgaaccaa cttagcgatt
    10141 tcacgtaatt ggtcataact aaaggtcgtt ggcagacgaa gaccaaaatc tttctcaccg
    10201 acatagatac ggtctacgcc agcttcgagt agttgttcaa cttgttcaat actttcagca
    10261 gttgctgtaa tgacaatctt ttccataaga aaaattatac cacatttctc caaaatcagc
    10321 catgctttga aaatgaaaag ggactttatt ctttttgtaa cctttctgta ataattctct
    10381 gccgaaaaaa tgataaaata ggtatgtact gttaaggaga gaatcatgcc cgtaagaaaa
    10441 ttacaatcct atgaggtaga ctatcaagaa gaattaaacc agcagcttcc tcattaccaa
    10501 gcttatacac ctgaagcgca atctgatgcc aatctcaagg aaatcctatt ttttattaat
    10561 atcgctgttt tttgtatctg tattgctatc tttagtttta tctttttagc attaaaatta
    10621 tcaactgctc ttgcctttgc cgcagcaatc ggattcagct tacttgtttt aaaagttcaa
    10681 cggtctatta tcaaacgaaa acgtagaaga taaatccaaa atatcgcccc aattggagcg
    10741 atatttttat tttttctttt tagatggtgt ctgaatggca atcttcactt caaagattgt
    10801 tcccttgggt ttattatctt tgacagtaac agttcctttt agagcatcta caatttgctt
    10861 ggctagggat aatcctaaac caaaaccacc tttttgccgg gttctagcct tgtctactcg
    10921 ataaaaacgg tcaaaaattt tctttttatc ttctgtcgaa ataccgattc cattatcaga
    10981 aacaagtaaa taaagattgc gatcggtcgc cgagataaga aaatcaattt caccatcctc
    11041 ctcagtatac ttgacggcat tatcgaaaag aatggtcatc agttgtttca gaagaagctg
    11101 atctgtgaca attgttcgat ggatacgatt ttcaaaacgg aagacacgat tattttccga
    11161 agcaatcatc tcatagtttg tgaaagttgt attaaaaaag ctagttggaa cttctgcaag
    11221 ctccggctta atcccatcat ctctccgagc taagttcagc aagcttgtcg ttaaaaaacg
    11281 catatttcgg acttcttcca aactcgatgc aatgctttcg ctcacatcca taatggtagc
    11341 ttctggctta cgaaaaaggg tctctaagcg attttgcaaa actgcgagtg gagttcgtaa
    11401 ctcatgactg gcattttcca caaaagactg ttgcttctgc atactctcaa gcaggggcct
    11461 aacactgacc ctagctagat agagactggc aagtaaagac aaaatccaga aactagccat
    11521 cacgaccaca atcaattgct catgcttttg actagcctgc tccaactgac tggtattaat
    11581 caagacagca gcatacttga tattggttga aaccgaacta atattggtct ccatcaaaat
    11641 cacacgatag atttcttcct gcccatagct attaaaaacc tgaatctggt agatatgtcc
    11701 tagttctttc ttttctaact taatcttatc caagcccaaa aatcgatttc cagaaagaag
    11761 ttgagtaaag tctttatcaa agagaatgac ttccgtattg gaactgacat taggttttat
    11821 ttctacttta ctagcgtccg ccctagcatt ttctaaatct ttaatctctt ctgttgccct
    11881 atttatagcc agctgaataa ctgcttgagg attttcactc agtccatgaa gcttatcgtc
    11941 caccgaagta taaagactcg aatgcatgac ttgtaaaata atcagagtca ttgtagaaaa
    12001 aatcagggtg aagacaccga agttgcggat aaaataacta aagtcatccg cataccatgt
    12061 ttttttaagt ttactgaaca tcttttaaaa gatacccaac actacgcaaa gtttgcaaat
    12121 tctctgcaaa agtggttccc tttaatttct tacggacttt tgaaacatag acttcgacaa
    12181 ccgaaatcgt tgtatcacta tcaaatcccc atagacggtc aaaaatctgc gtcttaggca
    12241 aaatcacatt ttgattttga aggaaataaa ctagtaagtc gaactctttc cccagcaatt
    12301 cgacaggagt atcttcaact ttaacggtat tggttgataa attaaccacg atattcccat
    12361 aagtcaaggt gttttcatta aacttccctg aacgtttgag aagggcctga atccgcattt
    12421 taagttcttc taggtagaaa ggtttggtca gataatcatc cgctcccagt tcaaatccat
    12481 gtcccttgtc atccaaactt tccttggcag tcataatcag aactggtgtc gtaattccct
    12541 tttcacgcaa ttcttttaag acttggaaac catttttttc tggcaacatc aaatccagca
    12601 aaatcaagtc atagacacca ctctcagctt cgtagagacc ttcttctcca tcaaatacct
    12661 gcataacatc cgcaaaatcg tctaaaaagt caaatactga atttgacaga cctaggtcat
    12721 cctcaaccaa taagattttt atcatgagaa actcctcctt attaaaacta ttataccaaa
    12781 tttgccttaa aaaaaactca actctctgca ttttacatga gatagctgag ttttcttttt
    12841 attttaggct tatttatgca tttccgtatt gaagaacaac tgcttcgact gcagcttttt
    12901 cacggctaat caagtcaaca cgtgctgcaa tttccttgat tcccataccg atgttacggc
    12961 taagagcaag gtcagaaagt tgcggctcaa agaactcctt gtattccgcc aagcgttgct
    13021 gagtcttaaa tacatgagca ggaaggataa caaagctatc aaagctcata tctcctccaa
    13081 gggctgcctt aatccaagcc cagttttcac gcgcccaaga ccaagctgtt ttctgagttg
    13141 cttgatgagc taggaattgg taataccaag cagacaagtc ctgtggtttg accacaaatt
    13201 tgtccttcca agaagtaatc aggttttgga tattatccgc atctgtactg tatgcaagag
    13261 ctgctgccaa ctggcgttta aagacagcat ctgttgcgtg agtataagta tcaagataaa
    13321 gtgctaacaa gtctttagtc tcatgatgtt tcatctcatt aatcagaact tgtgagcgaa
    13381 tagctgctgg gagtcctgca agattctcct tgtgtgttgc gaagatttgg ctagcgactt
    13441 gactagcttc tgcatcattt gagcgaatca tcatcgaaac agccagctga cgaaccaatt
    13501 catcctcatc tgattctccg tctttagctt caaaaccaag acggtcatag ttatgacgag
    13561 ccaatttagc aaccagtcct ttgaaggctg tttcagcatc cgttccttca tcaataaagc
    13621 gctcaagggc tgaaatcact tgagaaacag ctgaaaccac cagataagac tcttccttag
    13681 caagtttatc aagaactgga agcaagtctg cataagaaat gtgccctgcc tcagccaaca
    13741 aacgacgttc ttgaacaatt tgcagtttgc ttgtgttatc aagtgtctct agctcagcaa
    13801 gaacagcttc taacaagtct ccttgatagt cggtaatata gtgggcagta ttttcagtgt
    13861 tgagacgaag agctccttca ttttcagcaa gaagagctgc gtagccaggg atttcgatac
    13921 tttcagtttc gagtgtatca ggcaagcctt tccagttgct attgaggggc accacccaga
    13981 gacggttctt gtcttcgttc tcaccgatga agaattgttt ttgtgaaatc ttcaagacat
    14041 cattttcaac tttgacagta agaactgggt aaccaggctg ttccaaccaa gaatccatga
    14101 aggctgcgac atcacgtcct gacgcttgac caagggcatc ccaaaggtca ctaccaatgg
    14161 tgttgctgta ttggtgtttt tcaaagtagg cgtgcaaacc tttagcaaaa tcagcatctc
    14221 ctagccaacg gcgaagcatg tgcatgagac ggcttccttt ggcatagacg atagcgccgt
    14281 caaagagtgt attgatttca tctggatgtt taacttcgac gtggacagac tgaacgccat
    14341 cagtagcgtc acgttcaaga gcaagaggta ctccacctgt ttggaaatct tcaaagatat
    14401 tccagcttgg ttcgatggta tccacacaga cgtattccat catattagcg aaactttcat
    14461 tgagccaaag gtcatcccac catttcatag tcacgaggtt cccaaaccat tggtgagcca
    14521 attcatgggc cacaacaagg gcaacttgtt gacggctagc aaatgtagag ttctcatcga
    14581 caaccaagta aacttcacgg taggtcacaa gaccccagtt ttccatagca ccagctgaga
    14641 agtcaggaag ggcgatgtgg agagattgag gaattggata cttaactcca tagtaatctt
    14701 cgtaaaactc gatagagcga acagcgatat ccagtgagaa atcaagattt gaaagtggat
    14761 gtgctttggt tgagtagaca cctaccaggg taccattttt agttttagcg gtcacccctt
    14821 gcaaatcacc agcaacaaag gccaacaagt aagaagacat gcgaggtgtt gtctcaaact
    14881 tccagatacc tgtttcctta cggttttcaa catcgatttc tggcatgttt gacaaggcca
    14941 attcaccttc tgcttggtca aagcgaagag agaggtcaaa agttgctttg gcttcaggct
    15001 catccacaca tgggaaagct tcgcgcgcaa aatggctctc gaactgagta gacaagacct
    15061 ccttcttgac tccatcaact gtataataag aagggtaaat ccctgtcatg ttgtctgtaa
    15121 ttttaccaga aaaggcaaga accaattcaa cttgaccagc ctcagccaat tcgatatgaa
    15181 gggcttcatt gtcatggtca actgtaaatg gacgagcttg acctgcaact tctacagagg
    15241 tgatttccaa gtctttttgg tggagggaga tgcggtcact ctgtgcttga ccagtgatgg
    15301 tcactttccc agaaaaagtc ttggtctcac gactcaaatc taaaaataaa tcataatgtt
    15361 caggaacaaa ttgcttaata aaatgttcaa ctgcttgcat agttttctcc tattctaagt
    15421 ttaagagctg ctgctcttct tcaaacaaac ttattatatc atattttgct tgagaaaaaa
    15481 ggattggaca gcgatttcca aaactttctt ccttctaata gtctacagtg ggtagacctt
    15541 gcgaaattct tctaaaacaa ccttgctttc agatgtaaaa gtcagcccta cttcacccaa
    15601 acagtctgtg aaaaagtcct catgaacctg gcgccaatag gcaagtgatt tgtctccctc
    15661 accttcctta aaggcatgtt gcgcagaaac ttgattgaag agttcaacag aaacctttgt
    15721 aatttcgaca acgcagacag cctgattttg actatctaaa atgacatcga aggtccctac
    15781 ttggggaagt ggttcgtctt ctagtacata gaggtcatag gctgatgctg ttgctgtctt
    15841 ttctccttta aacaccaaat ccgctaaaag gtctggttca actccaaaag cccaagcatc
    15901 gatttcatct ccgatcaaag gattgatttg cttgtattta ttccacattt cttgcggtat
    15961 catgggtact cctttgtaat tttttacttt cttcttttat gtgtttaaga tgatctggat
    16021 ggtcaatctc taaatcaaaa atctctggaa tagaactgta gtggataatg cacttgatac
    16081 ccaactgatt cattttttgt atgaaagaag tattcagata gcctgctaca gcaaaattaa
    16141 tcttgttctt tcttgcttta tcctgcatat ctcttagcat atctaacatt attggacttt
    16201 ccatatcatg ccattgactg tttctcatag tcgcaaaaac aaaggaagtc aaatcattca
    16261 ttccaactac aatctttgaa atgcccgttt ccagtatact agataagtca aaatacgctg
    16321 acggtaattc aatcatcgtt ccgactttcc cagtaaagcc atactgacgc aatactgtaa
    16381 tagcttgttt taattggtcg gcatcattga caaaaggaaa aataagagac agattgggat
    16441 tggtttgata aacttctgta acgacatgtg attcagcctg aaattcatcc aaacacgcca
    16501 gtaaacgcct ggttcctcta tagccaaaca agggatgccc ttcgtcaaaa aactctctag
    16561 tccccgctaa acaattggct tctgtattcg ttaattcaga aaaacgatac caaacttcct
    16621 tatctaagta aaaggagcaa atagtatcaa gataatcttt tacaaattgc tgacaacttg
    16681 ctaatatgat gttttgatta agctctctca ataagtattc cccacgaatc atgccgacgt
    16741 ggtgaaatag ttgaggataa attttttcaa gaattttttc gccactaagg gcaagttgat
    16801 ttctcatagc cattcacctc gtcttaaaag atacaatatc atatataatt atatcaaaat
    16861 ttcctctagt tcaaaagctt cataaggttc agataactca tatcctaaaa attctgctac
    16921 ttttttggaa gcctcattat gagcatccca aagtggaaac atatctctat ttaaactctc
    16981 tagaatcatt gcagcaccaa gctttttagc aaatccattt ccttgttcgt ttggtctagt
    17041 tgcaacttcc acttcaactg ctttacggta aactaaccct gaggaaatcc cagcaatcag
    17101 ttcattattt ttaagaatca caaagccaaa tccacctttt aaaacaaaat cctgatagga
    17161 ctcaaaatcc ccctgtaaat cctgtgacca ttcttccgta gaaaagcagt tataaatatg
    17221 attatcaata ggcacaatat tgtaaccttc ctctaaatga gtaactagat tatttaaaaa
    17281 ttcaacttga aaatttgcct tatctttaaa agaatagcga gtaaaagaat ttagccccac
    17341 ttttcgatgc agaaaatctt gccaattagt attttctgaa ataataatct tataatctaa
    17401 accatatttt ctaacaaaat cttcccaaaa tctggaatcc aactcccctg ctaggtatag
    17461 aaaattccca acatcataaa agcttgttgt cctatcattg tttcggtaga cagttccaat
    17521 ttgagattta agaccataga gaaccatatt tttcttccat gttttaaata gttccattat
    17581 ccctctactt caccttccaa ttcatgtaag aagtcttgtc cagttctgga aatcctaata
    17641 attcagactt aaccttcaag actaatggcg atgcattttc ttctgtaatc tcttgaatat
    17701 ccatccaaat atatccaagt gaatcattcg caccatcaga cacagcttcc gaagtcgtaa
    17761 cttgaggtgc actctcattc atttcaacat catacaaggc catgacatgg tgaaccataa
    17821 aatttgttaa ctcttctctg acgaaaacat cgtagattcg aggattagag tagcttctaa
    17881 cagtaaatcc cgtctcttcc ataacttctc tagtcagcgt ttccgtcagt ccttcaccaa
    17941 gttgctgact gcctccaggt agatcatacc gatgttgata agggcctctc gctttttcaa
    18001 tgcagagtaa cttcccattt tctaagcaaa cagcgtagac tccaaagtgt tttttgattt
    18061 ccatccaact cctcctactt caaagaccag ccaccatcta ttgtcaagat ttgtccttgc
    18121 atggcgctcg cttttccact tgctaaaaaa agactaagct ctgctatttc ctctggctca
    18181 atccagcgct tgattggggt ttcactagcc acccagtcag ccaaaccacc tggttcaaaa
    18241 tccgcagcgg tcatagctgt cttgactgct cctggagcga tcccaaagac ctgaatccca
    18301 gcttcagcat agtctagagc caactgcttg gtgaagccag ccaaggcatg cttggatgaa
    18361 gtataggcgt gaccacctcc acctgctagg ctagaagcaa tggaacacat attgatgatg
    18421 attccctttt tattttccag catttgtgtc aaataatacc gagtcaactc tactggaata
    18481 atgtagttga tttcaaaaat ctcttgaatg tcctgcgccg tttgttccaa cagtggtttg
    18541 taatcatcca aaactccggc agtattacac aaaacatcca cctgagggca ccagtcaaaa
    18601 ataggttcca agtccaaggt caaatctctc tgtaaaaagt gaaaatcacc ctctaagagt
    18661 ggcttttcac cttggtcaac tccataaact tgatagccct tctctaaaaa gaggcgagct
    18721 tgagccaatc cgatccctga actcactcct gtaatgagta cacgtttagt catgcacttc
    18781 tacccaatcc gttgccaaaa catcacaagc tgtcgggctc cacatggaaa aaccttctcc
    18841 ttctccagac acgttgatta ggaaataagg tgtcatttca agtgcaagcc cattttgctc
    18901 gatggtatca aagagttgga catagttttc cgcacctccc caaccagttc gtacatattt
    18961 tctcttagcc tttaacccag gcagaatctc ttcaaatgtc atgtttttct cctttaattc
    19021 tacattcttc atttaattat aacaaaaaaa ccgctttaat acggcttttt gactgtgatt
    19081 tatttacatc ttcttctact tacggcaaat tattccctgc agcaagataa atttcatacc
    19141 attcttttct tgttaagcta aagtttgccg cttggctaac ttctctcaag tgcttaggat
    19201 ttgtggtacc tacaactgcc tgcatttttg ctggataacg caatatccaa gaaatggcaa
    19261 tagttgaaga ggttactcca tatttaatag ctaaacgatc aagtacttga tttaaagctt
    19321 gaaatttctc atttccaaca aaattccctt taaaataccc gaattgcaag acagaccatg
    19381 cttgaatgac cacatcgtgt aattggcaat attcaaaaat gctgccatct cgcatagctg
    19441 cttgactatc ttccatatta acatgaaaag ctgattcaaa tcctggagta aaagccgcac
    19501 tcaattgtag ctgattaaca gctaacggct gcttgacatc ttttttaagc aactccatca
    19561 tcataggatt ttgattagaa actccaaaat ctcgaacttt accttgttta taaaggagat
    19621 taaaggcttc tgctacttgg tcagattcca tcaaagcatc tggtcgatga aggagcaagc
    19681 tatctagatg atcaatcttc aatctttgca aaataccgtc tactgatttt ataatatagt
    19741 ccttagaaaa atcaaaatag gtaaattctt caatgcgaat gccacatttg gactgaatcc
    19801 acatcttttc tcttaaatct ggacgatttt ttaggacaag acctaacagt tcttcacaac
    19861 gaccacgacc ataaatatca gccaagtcga aggcattgat tccaacagaa agtgctgttt
    19921 ctacaagctc ttcaacttct tttacagact tatcttttat tctcatcatt ccgagaacaa
    19981 tttctgataa ttctttgtca tcttgaccaa gagttatgta tctcatcaaa tttttctcct
    20041 ttaatttcta acattcttcc cttcattata acaaaaaacc gctttgcaac ggctttttga
    20101 ctatacttca ctccatttta tcttcttaaa cccacggaac aagacaaaga ttccaataaa
    20161 gaggacagct aaaggaataa cttttgtaag gaaaacattt gaaattccca tccactcata
    20221 ataacggagc agagaaccca ccacaagatg ggcaataatc atactgacaa atggacgaaa
    20281 gaccgcttct ttccaattcc aaataccgat aactagcgaa atcgtaaaga cagacaaact
    20341 atcccaggga gccggaatat aaaaggctcc ttcttgtatg aagcttgcca ttcctacata
    20401 tcctaaaaca actagaagaa ctatagtccc aacaacaatg taagtgccaa ttttcatttt
    20461 aggagaatct tggactaaac ttcttcgtaa aattgtggcc acaagtccaa atccaatcag
    20521 aaaaataaga agttgcccta aaaatgtgag caaattgact gttaagagag gacctttaga
    20581 aaaatcactt agtagttgat aataacgtaa taccgccagg acaagaattg gcgtcaaaag
    20641 ggactctttg atagaactgc gaggtgctcc cttgagaatc tctttcatta tttttttagg
    20701 attcttacct agataatcct ctgcactcat gccatctcgt tctgcttctg agaaatctag
    20761 catcatcaaa tagatctgct ctctgagata gtcttcatca tagagaaatc cagcaagatt
    20821 aaaactttcc cacaactcct caaaatactt ttgattctcc tcagaaaact catgtagcaa
    20881 agcgcttgtt tcttcgtaat acttcatttt cttcatggtt taacccccat tcttaatccc
    20941 ttctactttt tgactcaaat cgtcccattg ttgccaaaag actgagacac gctcttctcc
    21001 ttctttcatt aatgaaaaat acttccgatc tggaccatct ggcgacgggc gcatgtcgcc
    21061 tcttatccat tgattttttt ctaacttttg caacaaagga taaatagttc ctggaacgat
    21121 agtatcaaat ccagcctctc gcaaagtctg aaccaactca taaccatacc gctctttttg
    21181 accaatcata tccaagacac aaccttcaag aacacctttt aatagctgag tttctttcat
    21241 cacttctccc ttctaatcta ttttgtaata cctactagta acttcaccta tagtatatca
    21301 cttctacact agtttgtaaa gcataatagt taatactctt cgaaaatctc ttcaaaccac
    21361 gtcagcgtcg ccctaccgta tgtatggtta ctgacttcgt cagtttcatc tacaacctca
    21421 aagcagtgct ttgagctgac ttcgtcagtt tcatctacaa cctcaaaaca gtgttttgag
    21481 caacccgcgg ctagcttcct agtttgctct ttgattttca ttgagtataa ataaaaaaac
    21541 agaactagcc tgaactagtc ctgtctactt ttacccaatc acacttccat ttggtacagc
    21601 tggatcaact gtgagaaggg ttaatttgcc atcatgttca gctgagagaa tcataccctg
    21661 gctgacatat tttttcatca ttttacgtgg tttgaggtta gcaatgattt gaactttctt
    21721 gccgaccaat tcttgttcat ttggatagta ttttgcaatt cctgaaagaa tctgacgatc
    21781 ttctccatca ccagcatcca agcggaattg aagcaactta tctgaacctt ctactttaga
    21841 cacttctttg acttctgcca cacggatttc aaccttgtca aagtcttcaa acttgatttc
    21901 atccttgttt agtttgagct caacttcgtc cggattccat tctttttcga ctgctggttt
    21961 attgccttcc atttgttcct tgatataggc gatttcttct tccatattta gacgtggaaa
    22021 gataggtgtt cctttggcaa ctacagtcac atctgctggg aagtcagcca aactcaagtt
    22081 ttcaagacta gaaacttctt ccaaaccaag ttgagtcaaa actgcacgac tagtttccat
    22141 cataaatggt tcaatcaagt gagcaactac acgaatgctg gctgccaagt ggctcatgac
    22201 acttgccaat tggtcacgaa gagcttcatc cttggccaag atccatggtg cggtctcatc
    22261 gatgtattta ttggtacgag agatcagagt ccagactgct tcaagcgcac gtggatagtc
    22321 aactgcttcc atgtgtgtat ggaagtctgc gattgattgt tctgcaacct cagcaagaac
    22381 atggtcaaat tcagtcacac cttctacata ggcagggatt tgtccatcaa agtacttatt
    22441 aatcatggaa accgtacggt taaggaggtt cccaaggtca ttagccaatt catagttgat
    22501 acggccgaca tagtcttcag gagtaaaggt tccgtctgaa ccaactggaa ggttacgcat
    22561 gaggtagtaa cgaagtggat ctagtccata acgctctacc aacatttcag ggtaaacgac
    22621 attccctttt gacttagaca tttttccgtc tttcatgaca aaccaaccat gggcaatcaa
    22681 acgatcaggt aatttaacat ccaacatcat aagaaggatt ggccagtaga tagagtggaa
    22741 gcgaaggatg tcttttccta ccatatggaa gactgttcca ttccagaact tgtcaaagtt
    22801 accatgttcg tcttgagcgt agccaagagc tgtcgcatag ttaagaaggg catcaatcca
    22861 aacgtagaca acgtgttttg gatttgatgg gacaggcact ccccatgtaa aggttgtacg
    22921 agataccgcc aaatcttcca aacctggctc gatgaagttg cgtagcattt cattaagacg
    22981 accatctggc gtgataaatt caggatgagc tttgaaaaat tcgaccaaac ggtcttggta
    23041 tttgctaagg cgaaggaagt atgattcttc agaaacccat tcaacctcat gacctgatgg
    23101 agcaatacca ccagtcacat ttccagcttc atcacggaaa acttctgcca gctggctttc
    23161 tgtaaagaat tcttcgtctg atactgaata ccaaccagag tattcaccca agtagatatc
    23221 atcttgagca agtaagcgtt caaagacctg tgcgacaact ttttcatggt agtcatcagt
    23281 tgtacggata aatttatcgt atgagatatc tagtaattgc cagagttctt taactccaac
    23341 cgccattcta tcaacataga cttgaggtgt aataccagct tcttccgctt tctgctggat
    23401 tttctgacca tgttcatcaa gacctgtcag ataaaataca tcgtagccca tcaggcgttt
    23461 gtaacgtgct aggacatcac atgcgatagt tgtgtaggca gaaccgatat gaagtttccc
    23521 agatggatag taaatcggcg ttgtaatata aaaattcttt tcagacataa tttttccttt
    23581 ccaggcaaat gaaacctgtt tttctaacac ttcattatat cacattttta atgaatttcg
    23641 atagggaaat ccatacaaaa acaagataga cgagtgtcca tcttgttgat cttattcata
    23701 acgaagggct tcaattggat caagtttcga tgccttgttg gctggcaaga ctccaaaaat
    23761 cataccaaca ctagccgaaa ctgcaagact aaatagggca actgggattg atactccaac
    23821 ttctatacct tctattaaac cttgcagtaa caaacctgct aaggcagtta aaccacttgc
    23881 aattgtcaag ccaattaagc cacctaacaa ggtcaaaatc atggattcaa tcaaaaactg
    23941 aattaaaata ttggcacgtg ttgcacccaa agccttacga agaccaatct cacgagtgcg
    24001 ctctgtcacc gaaaccagca tgatgttcat gacaccagtt cctccaacaa agagagaaat
    24061 ccctgcgatg gaactaataa tcgtcgtcat aaaactaaac gattgttgaa tttctgcaaa
    24121 tacaacggac tcatctgcca cctggtattc tccctgttgt aagcctgcaa gctctgtcat
    24181 ttttcgtgcc agttctggac ccagagttgg ggttaaactg gtatcattca ctcgaaagac
    24241 aatattagct atttcatcta cattaaaatt cgcagcaagg gagatattgg tagtaatagg
    24301 caagccacca aacccatata tttttgatct tttagcctcc ggactagtat aaaccccaat
    24361 gacccggtaa ctaaatccat tgacttctac aaccttgtta atagcctctt gaggagattc
    24421 aaataaacta atggacaatt cctcatctag caaaatgaca cttgcaaact ctttgaaatc
    24481 ttgctctctc agactacgac ctgcaataat ttcattctta acagcgtcca tgtaagttct
    24541 gtttccacct gtcaaattag cattctcaac ctttttatct tgataggtca agatggcatt
    24601 cgttgaattg gttacatagt aactatccac tcccttcagt ttagctgcct cttgaaccca
    24661 ggattcttgc ggttttggcg gttcaacagg aacttcctct tcctttccag aaaccgtaaa
    24721 agctgattgt ttctgagtaa aagacccatc tttacttttt ttaggagaga aaaagacgct
    24781 aatatttttc tgagatttag tcatatcttt attgacttga cgagataggg aatcacccaa
    24841 agccataatc acaacaactg atgaaacacc gataataatc ccaatcatag taagcaaaga
    24901 acgcatcttg tgagccatga tagatgaaaa ggcaaatttc agattctgca tcttagtttt
    24961 cctcctttcc taactgagca ctgtcagacg aaatgacccc atcccgaatg acaatctgac
    25021 gtttggcata gacagcaatc tcaggctcat gcgttaccat gataatggtt tttccttctt
    25081 tattcaaatc aaccaataat tgcataattt ggttacctgt tttggtatcc aaggctcctg
    25141 tcggttcatc cgctaggata atagaaggat tgtttaccaa ggcacgcgca atggctacac
    25201 gttgcttttg accaccagat aattctgaag gtaaatggtg actacgttct gtcaattcaa
    25261 ccttgtctaa atattcctca gccaacttgc gacgttttga agacgaaact cctgcgtaaa
    25321 tcaagggcaa ttctacattt tgcagagcat tgagcttcga tagaagaaag aactgctgaa
    25381 agacaaaacc gatttgttgg ttacggacct tagctagttg tttttcacca agcccagcca
    25441 cttcttgacc ttcaagataa tattctccac tggttggtgt atccaacatg ccaatcgtat
    25501 tcatcagagt ggacttacca gacccagatg gtcccatgat ggctacaaat tcaccctcat
    25561 tcacttctag attgatattt ttgagaacct gcagttcttg gtcaccatta cggtaacttc
    25621 tgaagatatt ttttagacta attagttgct tcatcagcct tcacctcttt tccttcttcc
    25681 aaggaagatg ttggattact gatgacctta gcaccgttcg ttaaaccaga agtgatttct
    25741 tgattttctg cgtcagcatt tcccaatgaa acctcaactt ttttagcctt ttgttgttca
    25801 tccacaatcc agacataatt tttactatca tccattacta gactgctaac aggaacaaga
    25861 atagccttag ttttgctttt aacctcaatg ttgacagaaa aaccttgttt caaatcacca
    25921 acctcgcctg tcacatcaat agtataaggg tatttagaac ctgtattatt cccggctgct
    25981 ggactagctg cttcaccatt gtttttagga tagtcagaaa tatagcttaa tttcccagtc
    26041 cattttttat caggatacac tttagaagta aagcttactt cttgacctac agaaaggttg
    26101 gctagattgt actcagacaa ttctcccttg acttgtaaat tttcattgct gacaatatga
    26161 accataactt gactcgcccc tgttggagat ttagaaacat tgctattgac ttcgactaca
    26221 gttccctcta gggtactgag aacagttgtt gcatccaatt gactttgagc cttgcttaat
    26281 tgcgctgcag catctgcacg cgcatcacgg gcatcaccca attgagcatc aatagaagca
    26341 acagaatttc cagccactgg agttgggctt tgcaccgttg catcttctcc tcctactggc
    26401 gctggtaact gtggaacctg agctgaagcg gcttcatttc gtgcttgatt gagttcattg
    26461 atatgacgat ctgccttagc tactgctcga ctagctgaat cataggccgc ctgcgcttct
    26521 gaactactgt acttgactaa agcctgccct tcgctgacct tatcgcccac agaaacaagg
    26581 atttcatcta aatcaccctt actagcatca aaataaacat attgttcatt ttttgctgtt
    26641 actgtccctg acaataaaac agaggatgcc acgcttcctt ccttggcaac aacaagatga
    26701 gtaggctcat cttttacagc agtctgagaa ggttgtctaa aaagtaaaat ccccccggca
    26761 cccaatacaa ctacactcgc agcaccgatt gctgcataca gttgccactt tttagcttta
    26821 ccattctttt tcttcataat gaaactcctt ttctttttta caatactttg ctattatacc
    26881 aaatttccct ccagcaaaca atgcagttca gatggaaata atggcagttc aggattaaac
    26941 aatcgttcgg aattttgctt ttctattgta ctagatatat aatacactat cttgacttat
    27001 ttggcaagct tttttgcaat tttttgtgcg tagcagattt ttgcaatcaa atcaaaaaaa
    27061 gaataaaata taactatcaa aaaagacgct ctactattta aaagattaca aaggagtttt
    27121 caatgagaga atatgatatc attgctatcg gtggaggtag cggaggaatc gctaccatga
    27181 accgtgctgg tgaacatgga gccaaagcag ccgttattga ggaaaagaaa ttaggtggaa
    27241 cctgtgtcaa cgtcggttgt gttcctaaaa aaatcatgtg gtacggggcg caaatcgctg
    27301 agactttcca tcaatttgga gaagactacg gctttaagac tactgatctt aactttgact
    27361 ttgcaaccct acgtcgcaat cgtgaagcct acattgatcg cgctcgttct tcttatgatg
    27421 gtagttttaa acgcaacggt gtagacttga ttgaaggtca tgctgaattt gtagattctc
    27481 atactgtaag cgtaaatggt gaactgattc gtgctaaaca tatcgtgatt gctacaggtg
    27541 cccatccaag tattcctaat attcctggtg ctgagctagg tggctcttct gatgatgtat
    27601 ttgcctggga agaacttcca gagtcagttg ccattctagg cgctggttat atcgccgttg
    27661 aattagctgg cgtactccac acttttggtg tcaagacaga tctctttgtt cgccgcgatc
    27721 gtcctttacg tggttttgat tcctacatcg ttgaaggttt ggtcaaggaa atggaaagaa
    27781 taaacttgcc acttcatact cacaaagtcc ctgccaagtt agaaaaaact gctaaaggca
    27841 ttaccattca tttcgaggat ggtaccagtc acacagctag ccaagttatc tgggctacag
    27901 gtcgccgtcc aaacgttaag ggcttgcaac ttgaaaaagc tggagtgact ctgaacgaac
    27961 gtggctttat ccaagtagat gaataccaaa atactgttgt tgagggaatc tatgctctag
    28021 gtgatgtaac aggtgaaaaa gaactgactc cagttgcaat caaggctggg cgtaccctat
    28081 ctgaacgtct ctttaacgga aaaacaactg caaaaatgga ttactcaact attccaactg
    28141 ttgtcttttc acaccctgct atcggaactg ttggtttgac agaagagcaa gctattaaag
    28201 aatacggtca agaccaaatc aaggtttaca aatcaagctt tgcatctatg tactctgctt
    28261 gcactcgcaa ccgtcaagaa tcccgtttca aactcataac agctggttca gaagaaaaag
    28321 ttgtcggact tcatggaatt ggctacggcg ttgatgaaat gattcaggga tttgccgttg
    28381 ctatcaaaat gggagcaacc aaggctgact ttgatgcaac tgtggcaatt cacccaactg
    28441 catctgaaga atttgtaacc atgcgttaat cgaaacaaaa gaagctgata taaatatgtc
    28501 tgcttctttt ttaatgattt tcgaatcaac aaaaaaatta tccaacttga ctagtcacga
    28561 aaatcggctg ttacagtttt tgttaccttg tttttaaaaa atggttgaca tcgactccta
    28621 tatgagtata atagttagta tacattagaa aagaggttaa ctattttgaa aaaagctcac
    28681 gtttatgcta tccctgctat tggggctgct ctcattgctg ttttggcaca aatcagtctt
    28741 ccaattggac ctgttccctt cactctgcaa aactttgcaa tcggcttgat tgctactgtc
    28801 tttagaccga gagaggctgt actttctgct ggactctatc ttcttctagg tgctatcggt
    28861 cttcctgtct ttgcaggagg tggagctggt tttcaggctt tagttggccc tactgcaggc
    28921 tatctttggt tttatctcgt ttactctgga cttacttcct ctctaaccaa cagcaagagt
    28981 ggtgttgtta agatttttct tgcaaacctc ttgggtgatg cccttgtctt tgtcggcggg
    29041 attctcagct tgcatttcct agctggaatg gcatttgaaa aagctcttgt tgtgggggtt
    29101 cttcccttta tcattccaga ccttggcaaa cttctggcta ttagttttat tagccgtccc
    29161 ctacttcaac gccttaaaaa tcaggcttac tttactaact aaaaaaggat accgagttat
    29221 catgactcaa tatccttttt ttattttgaa aacttatact caatgaaaat caaagagcaa
    29281 actaggaagc tagccgcagg ctgctcaaaa cactgttttg aggttgtgga tgaaactgac
    29341 gaagtcagta accatacata cggcaaggca aagctgatgc ggtttgaaga gattttcgaa
    29401 gagtattagt tgcctttaaa ggcatccacc atcgtttgaa attcttcatt tgagagagta
    29461 atccctttgc ccattttagt atggtctgga ctccaagcac gaatatcaaa ctttgcaggg
    29521 gcaccattaa agctcacacg gttaatttcc ttggtccaac ctttttcgtt ttcagaaaga
    29581 gtcaacaagt gctcttcgat ttcaaatgta aattctgcca ttttcttctc cttttttagt
    29641 ttcattagtt tattcgtaaa atcttgtaga ttttaggaaa attttatata atattgatat
    29701 aaaagaaggg aggccaatat gagacataaa ttccagcaag ttctaaataa aatacatgat
    29761 tttttaaatg gatatgacca acctgaccag actgaaacca actcccttac agccactatt
    29821 gaagaggcta tccagaaaca aaccgctgtt caccttatct tatctgagac aagctttaca
    29881 ggtgacatca tcaaatatga tcagcaaggc cagcaaatta tcgtgaaaaa tttttccaaa
    29941 aatgtgagcc ggattatccg tataagcgat attcaacgcc tgcgatttgt cccctcaact
    30001 gtccaaacag cccaaaaaaa tagatttaag aaagagtgag atgtagttgc ttcatcccac
    30061 tctttttctt agcgaatttg ttcaaaatgt aaatgaactg cgatatgatc tccataacca
    30121 cttctttcca agtcacgttg taaacgatag gaaatatagt gttctgcaat ggtaatgtaa
    30181 cctgcgccca ataaacgatg ttcaaccata gattgaatca tactgatagt cgcacgttcc
    30241 accttggctt cttgtaaatc caaaactacc ttcttagtga cttgagcaag attttgacgc
    30301 aaatcatctg tcaaaacata aacagtttgg gctgccttca agatggcttg gtaaatctta
    30361 tctggattaa attcagcaat ttcgccatta cgtttgatta cttgcatagg tttctccttt
    30421 attctttgtt ttctttgatt tctgccagca ttttttcttc ttctactgtc agttgataat
    30481 gttcaagtaa atccggtctg cgctcgtagg ttttctttaa actctcgtac aatcgccact
    30541 gacgaatctt ttcatggtgg ccactcatca atacatctgg cacgaccatg cctcgataat
    30601 catagggacg tgtgtactga ggatattcta aaagacctga agaaaaacta tcatcttggt
    30661 ggctagactc cttgccaatc acttctggaa tcaggcgaac tgtagcatca atcatggtca
    30721 tagctgccaa ttctccacca gtgaggacat agtcgcctag ggaaatctca tctgttacca
    30781 aggtcttaat gcgctcatca taaccctcat agtgcccaca gataaagatt agctcttcct
    30841 cttgagccaa atcttcagca taagcctgat caaactgctt tccagcagga tcgaggagaa
    30901 taacgcgcgg atttttcttt tcaatagcat caaaggaatc gaaaataggt tgtactctga
    30961 gcaacatgcc ctgaccgcct ccgtagggct catcatctac atgacgggcc ttttcagcat
    31021 tttctcgaaa attatgatac tggatatcca agagcccttt ttctcgagcc tttccaacga
    31081 ttgagtgctc cagtggagaa aacatctctg gaaagagggt taaaatatca atcttcatcg
    31141 tctaaccctt ctaagatttc cacatcgacc cgtttatttg gaatatcaac attgagaacc
    31201 actggtggga tataaggtaa aagcaaatca cgtttgcctt ttcgtttgac cacccagaca
    31261 tcattagcac ctggttgcag gatttccttg atggttccaa ccaagctatc accctcatag
    31321 acttccaaac cgataatctc gtgatagtaa aattcaccat cgtctaggtc attcaaatct
    31381 tcctcagcga ccttgagact gtatcccttg tacttttcga tagtattgat atggtacata
    31441 tctttgaatt taataatgtc aaagttcttc tgtttacggt ggctagcgat ggtcactgtt
    31501 tggacaaact gatctttttc atcaaacaaa gccagctcag ctcctttttt aaaccgttct
    31561 tctgcaaaat ccgtcacaga caagactcgc atctcaccct gtaatccctg cgtattaacg
    31621 attttcccaa cattaaagta gttcatcttg tctcctgtaa tctccttttt tccatcttat
    31681 tctgacaatt ctcgaataat agccgcaatt ttttccgatt ctgaccattg taaataatgg
    31741 tgattccctc ctaaaatgag tttagtattg gaagtccaat attctgattc tctgtactct
    31801 ttttctctat aaggctgaca aaaaacaaat acaggaatat gagcttctat agatacatcc
    31861 tcaaaatctt cctcagtaat ctctccagat atctgaaatt ctggatcttg attttccaac
    31921 tctaagcctt tttcttgcat taattcccag atttttttat tcgtttcagg actaaatgtt
    31981 gcttgagtta aattcttcaa ataaagttca ggtccatact cgtcaatcag cctcatctgc
    32041 tcttccattt ctggataagg attttctgaa aaatcagcaa acatgacttt tttagttgtc
    32101 ggttcaattg ctactaaagt ctgacactta attggtttct cgagtaattt gcaagctaaa
    32161 attccactcc aactatgtgc acaaagtata tattcagaaa tccctaattc ttcaagtact
    32221 tcataaacca catctgcaag attatctaga ttttttccag cttggtcatg aatcggactc
    32281 ctacctgtgt tcggaaaatc aattgtcaaa taaccaattg taggaggagg tttttcaagt
    32341 ataagtgaaa aattttcata acttggtagc aaacctgctc cgtttagaca aactagcact
    32401 ttcttttgct tttgataagt aacagagagg ctaccaattt ctgtagatac ttcaaacctc
    32461 ttcataaaga aatccactca ttctatataa tgaattatta aaaatcctta tcctttattt
    32521 tatcacgttc caaggatttt ctcaagttgg aggaagggga caacatctct actttccctt
    32581 caagaatcct tccaaatcat gtttatgttg gtaattaacg gctgcggttt tgtctttctc
    32641 aaagacagtc ttggtaaggt caatatgctt gatagcagca atagcgacgg tgtagtaaat
    32701 gatatcagcc agttctctgg caagttcctc gttcgaatcc tatcccttct tttcgaccag
    32761 agcgcctatt caaaacctcg gctacttctc cgacttcctc cactaacttc ataaagagac
    32821 cttcatcagt ccgagactgc tgttaatgtt cgagtaagta gtcttggaat tgcctaaacg
    32881 ttcaatcttt tatagtatat tgaaactaga atagtacacc tttacttcta aaacattgtt
    32941 agaaatcgat ttgactgtcc tgatcgattt gtcctgttat tatttcattt tactatatct
    33001 tctattccac acaaaaaagc gagacatccg tcccgccctt cttatttttc gtcaataacg
    33061 attcttactt ttttgtattc agttgggaca gagtagacaa tcgttcttat cgcagaaata
    33121 gtgcgaccct tacgaccgat tacacgaccc acatcgcttt gatcaagatt caaatgatat
    33181 tccaaaaatt ctggtgtatc ttcaatcttg atagttaagg catctggttg tgaaattaag
    33241 ggtttcacaa tcgcaataat gagattttca atcgtatcca tctgtcaacc tactttaaac
    33301 ttattttgag aatttagaat cgtggaattt tttcaatacg ccttcttttg aaaggatgtt
    33361 acgtactgtg tctgaaggtt gagctccatt agccaaccat gcaagaacgc ggtcttcttt
    33421 caaagttact tggttttcag caacaagtgg gttgtaagtt ccaactgttt cgatgaaacg
    33481 tccgtcacgt ggtgaacgtg aatctgctac gttgatacgg tagaaaggtt ttttcttaga
    33541 acccatacga gtcaaacgga ttttaactgc catttttaaa gtctcatttc tttaattttt
    33601 tatttcggtg aaatagctga gctatttagc acatgagcta tttagcacat gttctattat
    33661 agcagatttc tggcatgtgt caagaaaaaa acttgacaga ctataaattt cttaaagtat
    33721 ttagaaattt gttagaatag gaaataaatg tttgaaagat actatccgtg aatactattt
    33781 tagaaaaatt ttataaagaa caccaagtca aacccttcat atcgcctgaa cgagatttgg
    33841 atacttggct cctaagtccc aagcccgttc ccaagcgaaa tatgaactta ttagcagatg
    33901 attcactagc aggagatatc attttactat ggagaatcca gtttggtact tttaccactg
    33961 aaacatgatt ttaaaaactc ccattcgaat atgattcaaa attcgcttag aatcaatgtt
    34021 ttcccacttt ttcaaaaaat aaattttcat ctaatttaaa taactttcaa ttaagtgtga
    34081 attttgctta aaatcattaa aaaacacccc atggtgtgaa ccatgaagtg ttgtaaatgc
    34141 tgtgttgtag ctagttctta gcgtttagag aactggctag ctttacgagc tttcttaaga
    34201 tctggtttcc aaaatacttt gagtacactt atggtattga tgcccctaaa catctaaaaa
    34261 ctctggtaga aaaagggtat gctcttgtcg aaacttcttt tgactcactt gaccatctaa
    34321 atactaccat gaaaaagaat atcctgaaga agaagggagt tactggactt tctaaaatga
    34381 aatcttgcga cctggatcaa gcccttcatg agcatttttc agaagaagaa ttagctggtc
    34441 actttcatgt ccttctatgg acttttttac aatggcattg ctatcacacc caatacctat
    34501 ctaagcgcct ggttcgtaaa ctttattgca gctcttcctc taaatttcct aattgttgaa
    34561 ccaattgccc gttttatact aagttctttt cagaaaccat ttactgggga agaagttgaa
    34621 gattttcaag atgatgatga aatcccaact attatctaag ccagttctgt aaactactaa
    34681 tatttgaaat ccacttcctt ttagggtaca atggttataa atgaattttt gagaggatca
    34741 gaatgaaaaa actagcaacc cttcttttac tgtctactgt agccctagct gggtgtagca
    34801 gcgtccaacg cagtctgcgt ggtgatgatt atgttgattc cagtcttgct gctgaagaaa
    34861 gttccaaagt agctgcccaa tctgccaagg agttaaacga tgctttaaca aacgaaaacg
    34921 ccaatttccc acaactatct aaggaagttg ctgaagatga agccgaagtg attctccaca
    34981 caagccaagg tgatattcgc attaaactct tccctaaact cgctcctcta gcggttgaaa
    35041 atttcctcac tcatgccaaa gaaggctact ataacggtat taccttccac cgtgtcatcg
    35101 atggctttat ggtccaaact ggagatccaa aaggggacgg tacaggtggt cagtccatct
    35161 ggcatgacaa ggataagact aaagacaaag gaactggttt caagaacgag attactcctt
    35221 atttgtataa catccgcggt gctcttgcta tggctaatac tggtcaacca aacaccaatg
    35281 gcagccagtt cttcatcaac caaaactcta cagatacctc ttctaaactc cctacaagca
    35341 agtatccaca gaaaattatc gaagcctaca aaaaaggtgg aaaccctagt ctagatggca
    35401 aacacccagt ctttggtcaa gtgattggcg gtatggatgt tgtggataag attgctaaag
    35461 ccgaaaaaga tgaaaaagac aagccaacta ctgctatcac aatcgacagc atcgaagtgg
    35521 tgaaagacta cgattttaaa tcttaaaaac caaaaaaata cagtatccac attcggtact
    35581 gtattttttt tactctcatt cttaagttaa attattaaaa tcccatattt ggtctatcca
    35641 gccttcataa aagtctggct cgtggcagac cataaggata gatcccctgt attctttgag
    35701 agcgcgtttg agctcatcct ttgcatccac atccaaatgg ttggtcggct cgtccagcac
    35761 taaaacgttg ttttcacgat tcatcaagag acagaaacga accttggctt gctctccccc
    35821 tgataatact tgaatctggc tttcaatatg tttggttgtc aaaccacaac gggcaagggc
    35881 tgcacggact tctgcttgat taagggcagg aaaggcattc cagacagctt caagaggagt
    35941 ttggcgatta ccgccttcta cttcctgctc aaaataacca agttctaaat aatctccacg
    36001 ctccacttcc ccagcgattg gcgagataat gcccaagaga atcttcaaga gagttgtttt
    36061 tccaatacca ttagcaccaa taatcgcaac cttttgattg cgttcgaagg taagatttaa
    36121 aggcttagta agaggacggt cgtaaccaat ttgcaagttc ttggcttgga agataaagcg
    36181 ccctggtgta cgagctggtt tgaaatcaaa ggatggtttt ggtttctcac tttggagttc
    36241 gataatatcc atcttatcca atttcttttg acgagacata gccatattac gagttgcaac
    36301 acgggcttta ttacgagcca caaagtcctt gaggtctgca atctctttct gctggcgttc
    36361 gtaggctgcc tctagctgag atttcttcat agcataaact tcttggaact ggtagtagtc
    36421 accagagtaa cgcgtcagct gttgattttc cacatgatag acaatattaa taacgtcatt
    36481 gaggaatgga atatcgtgcg aaatgagaac aaaggcattc tcatagtttt ggagatagcg
    36541 cttgagccaa tcaatatgct cagcatccaa gtagttggtc ggctcgtcca acagcaagat
    36601 atcaggcttt tcaaggagaa gttttgccaa aagcaccttg gttctttgcc cacctgacaa
    36661 agaagttaca tccgtatcca tgccaaagtc cataacacca agagcacgcg ctacttcgtc
    36721 aatcttagca tccaaggtat agaaatcacg actctccaga cggtcttgaa gttctcctac
    36781 ttcttccatg agagcatcaa catccgcgcc gtcttcagcc attttcatat agaggtcatt
    36841 gatacgagct tcagctttga aaagctcatc aaaagccgta cggagaacat cacgcaccga
    36901 ctgtctttca gcaaggacag agtgctgatc caagtaacca gccgtcacat atttggacca
    36961 ctcaaccttt ccttcatctg gcagcatttt accagtcacg atactcataa aggttgattt
    37021 tccttcacca ttggcaccga ccaggccgat atgttctccc ttgaggagac ggaaggacac
    37081 atcttcaaaa attgcacggt caccaaaacc gtgactcaga tttttaactt ctaaaatact
    37141 cattttaatt ccttatcttg tttttatgta atcgtttata aaggagccaa gccagatagc
    37201 cacccaaagt gttggtccac aaatcatcaa tctcaaagac gcgattgaaa tcaaagaaaa
    37261 agtctaagat taattgcgta cactcgattc caagactcac aagaaaacta aaaagaagga
    37321 ccttttttgt tttccgcaaa tttggaaata gataaaggag ttggaaaatc agaggaaaaa
    37381 acaagaagac attgaggata ttttgtaaaa aaatccaaca taattgtcca atgtcactca
    37441 cttcgcccag tttccagaga gaattgaaag gagtcaaaag aaaaaccagg cgtccaagat
    37501 gctgaatacc tggagttccc actcccacgg tagattgttc ttgaggagta aagcaaaaac
    37561 agacaatgca aatgctatag aaaatgactc cccagaccaa aatatgatta taagtcttct
    37621 tcatcattaa ggatttaccg ctgcgactgc cttctggcgg tcacgtttca ttgtgttaaa
    37681 gcgcaattgt ccacaagctg cgtcaatatc tgtaccatgc tcttgacgaa ccacacagtt
    37741 gccccctttt ttcttaagcg tatcatagaa agccaacacg cgctctttgg gactacggct
    37801 atattggtca tgctcactaa ctgggttata aggaatcaag tttacataag acaatttctt
    37861 gatgttcttg agcaattcag ccaattccaa ggcttgttct acaccgtcat tgacttcatt
    37921 aagcatgata tattcaaagg ttacacgacg gtttgttgtc tcaatgtagt attcaatagc
    37981 agcaaagagt ttttcaatcg gaaaggcacg gttaatcttc atgatacttg aacgaagttc
    38041 attgttgagt gcgtgaagag acacggcaag attgatctga accccttcat cagcaaagtc
    38101 acgaatttta tgagccaaac ctgaggttga aaccgtgatg tgacgagcac cgatagccat
    38161 tcctttatca tcattgatag tacgaaagaa attcaagaca ttgttgtaat tatcaaaggg
    38221 ctcaccgatt cccatgacaa cgatatggtt gacgcgttca tcctgaccac gctcatcaaa
    38281 gtatttctga accagcatga tttgcgctac gatttcaccg ttattgaggt cacgctgctt
    38341 cttaatcaaa ccagaggcac agaaggtaca accgatatta cagccgacct gagtggtcac
    38401 acagacagat aaaccatagt gttgacacat gagtacagtc tcaattaaca taccgtcagg
    38461 caattcaaag agatatttga ctgtaccatc agcagactct tgcacaatac gttgtttcaa
    38521 gggattgacc acaaactggt cattgagctt agcaatcaaa tccttggaaa ggttggtcat
    38581 ttcttcaaat gactgcacac gtttacggta gagccattcc cagatttgat ctgcacggaa
    38641 tttcttttct ccctgctcca atacccattc ctgcatggtt tgatgtgcca aactatgaat
    38701 tgagggtttc atttcttctc cttattctct actcacttct gacgaatgac aaaatgacgt
    38761 tgttccttgt cctctttctg agaatgctgg tctttctgac gacgtctatt tttcttatct
    38821 gcattcgact ttcgtttagt ttgagtcggt ttctttcctt ttctagaagg tgtttcttct
    38881 tccgtcttac gcattttctt gtcaaatgat gctcgcttag gggcttcatt ttctaagaca
    38941 aaataggcac aaccataact acaatactct aaaaggtagt cttgtaaacg actgattttt
    39001 tcaagttttt cttctgttcg gtcatccttg taaaaacctc gtaggcgaag ctgttcgttg
    39061 ctccagtccc ccacgatata atcaaacttg gttaatactt ctgaaaaacg ctgattaaaa
    39121 gtcgtcacat caaaggcatc cttgatattt tcaaccaagg caaaagctat cccttccgtt
    39181 tcgaccttgt ccccgtgtaa atggaactcc ggaccaggaa acttgttata gttgtataat
    39241 tcaggtgcaa tttcttttcg catagatatc ctttttccac gattacttaa tactttattc
    39301 taccataatt tctagcagtt agcacgtttc tcataaaaat gaaaaaagtc tgacgatttt
    39361 gtcagaccag aatcttataa cctaaaaaag agaagaacaa ttcttccctc caactatcat
    39421 tatttagcag ctgcgtacaa ttcatctact ttattccagt tgattactga aaagaaagct
    39481 ttgatgtagt caggacgcac gttgcggtat ttcacgtagt aagcatgttc ccaaacgtcc
    39541 aagcccaaga ttggtttttt accttctgag attggtgtgt cttggtttgc tgttgaagtc
    39601 acttcaagtt tcccttcttt gttgacaacc aaccatgccc aacctgaacc aaaacgagtt
    39661 gttgctgctg cagtgaaggc tgcttggaat tcttcaaatg aaccaaatgt tgcatctatt
    39721 gctgctgcca gttctgctga aggagctgtt ttctcgggag tcatcaattc ccagaaaaga
    39781 gcgtggttca agtgtccgcc accattgttg ataagtgctt gacggatatc agctgggata
    39841 gattctacat cagcaagcaa ggcttcaagg tcttcaccga tttcagggtg tttttctaaa
    39901 gctgcattgg cattgttgac ataagtttga tggtgtttgt catggtgcaa atgcattgtt
    39961 tccgcatcga tgtatggttc caaagcgtcg tatgcatatg gaagttctgg taagataata
    40021 gccatctgta atacctcttt ttctttctat atgaaaatga taacgcaaac attcactttt
    40081 ttcaagtttt ttgcttatag ataaggaaat cactgacata ctttctaata aaataagcaa
    40141 atcagcaaga aacccacgac aagataatcc tgacgcgagt tgtagtttca acaaatgtca
    40201 attgacctga ctagcaatct gtaagagtgc cttttcaaaa aggaaacctt tttcataaag
    40261 acctgtctta atctgatagt ctgtctcaat caaataggaa atagcttgct tcagaaagct
    40321 caaagaaagt cctctcgaat ctcttaatgc aaacttgatt tgataaggat ttgggttacg
    40381 tcccagataa ctacctaaac tacttgcaat ctgcgattct gtttggccag actccgccaa
    40441 aatcttcacc tgagtaaaag tccgaaattg tcccagcatg actgcaatca gtttgatttc
    40501 atcttcccct tgcaaggtca agtctctcac caaatcgcgc gcctgatcca tctttttagt
    40561 cagaataaac tgagttaaat caaaaatatt gtcctgcaag gtcttgggaa ttgcgttaac
    40621 aatatcctct tcctcaataa cagaattcgc cttataggac tgtaaaaaga gaagattttt
    40681 ctggatttcg ctaaattgaa accccgactt gatgaggaga ttttcaaaag aatgattggt
    40741 aaactgcaga ccttgtttct gactccactt ttggaaatac tggcgcaatt cttgttcttt
    40801 tacttctact gcatcgaaga ccttggcatc acgcttaagt aatttgacta accgtctttt
    40861 gctatccagc tttccttctg caaagattat caacttggtt gttggagaag gattgtcaag
    40921 gtattcctca aatgacttaa gctcatcatc tgtcaaaaag cgtttcttag cagtcgtgat
    40981 atccataaaa taatctaata tcacgatttt ctcatccgca aagaaaggaa ggctgaccaa
    41041 ctccagttcc acatccttgt aaactacttc tttcatatca aagtaggcaa agttgaggtc
    41101 agcagaatca tacccaatct gtttcaacat ttgactcttc atcacttcaa actgaccctg
    41161 atctgtccct gtaaataggc tcaggctcgg taaatttgat aaagtcaact tctgactttc
    41221 ttcaatggct agcatcgtct ctcctttctt cagatttttt gatttaattt agtcaatata
    41281 gcgcaatttc ccacggaaat cttctaagct ctcgtagcct ttttccacca tgattgcttt
    41341 cagttcattg gtaatgcagt caaaagcact gacgccttct ttgtgaaggg tcgttcccac
    41401 ctgcaccata cttgctccac agaggatgtg ttcaaaggca tctcgaccag tcagaacgcc
    41461 acctgttccg ataatttgga tttgaggatt taaacgttga taaaaggcgt gaacattggc
    41521 tagagcagtc ggtttgatgt attctccacc aattccacca aaaccattct taggccgaat
    41581 aacgacagat tcgtcttcta tatagaggcc gtttccgata gagttaacgc agttgacaaa
    41641 cttgagcgga tatttgttga aaatagctgc cgcttgatca aagtgaacaa tatcaaaata
    41701 aggtggcaat ttaattccaa gaggtttggt gaagtaagca aacacttctg ccaaaatccg
    41761 gtctgttgtc tcaaaatcat aggcaatctg aggtttacct ggaacatttg gacaggaaag
    41821 atttagctca gtcagaccac gaaaatcact ctcttggact tttttcaaaa tagtatgggt
    41881 ttcctctgga gacatgccga ccagagataa gaagaaagtt cggttcgact ctttttcctg
    41941 caaatctaaa agataatcca aataatagtc taagccatta tttggcaagc ccatagagtt
    42001 gatggaacca agtggaacat cttggtagcg tggctcagga ttcccctgac ggaagtccaa
    42061 ggtcgctgtc ttagtaacaa aggttcctgc cgctgagttt ttgacctctt ctaactcctc
    42121 tatcgtcata caagccacac ctgctgcatt catcaagcaa ttgtcaaact caaaaccagc
    42181 aatttgtgtt ttcgttgata ccatgattct aatcctccag atttctttta ttgctaatat
    42241 tataccataa ttttaattct aggtttacta gattctgtgg ttctatctat ttattcggaa
    42301 aagaaagtct agtacagaag tcatgctatg gaaacagcta gtgtattgga agtttgaaca
    42361 aggcttgggt aacagacttt tgaatgaagt ccaagcaaca aggagaaatc tcaaccatga
    42421 gtccattcca tgactctgtt gctctagaaa accttgcaat agtcaagtga taacgaataa
    42481 aactttggct tgaaatatcc ccatgattat ctattacgag gagaaatctc aacctttatc
    42541 atttttaaag aaatattgta aaatgaaaca agaacaggag aaatcgatca ggacagtcaa
    42601 atcgatttct aacaatgttt tagaagttga ggtgtactat tctagtttca atctactata
    42661 catgtaaatc tctactttcg caaaatggat tttcagaaca gtatcccagt atagttaaaa
    42721 actataaaaa agagaggacg ttcccctctt aaaatcttat tttactgctt ctttcaaagc
    42781 atctacctta tccaaacgtt cccatggaag atcaatatct gtacgtccca tgtgaccgta
    42841 agccgatgtt tgacggtaaa ttggacgctt aaggtccagc atttggataa tccctgcagt
    42901 gcgaaggtca aagatttgac gagccgcttt ttcaagttga ctttcagcta ctgttcctgt
    42961 accgaaagta tcgatacgaa cagaaacagg ttgcgcaaca ccgatagcat aggccaactg
    43021 cacttctgcc ttcttagcaa ggtctgctgc aacgatattc ttggcaatat agcgagccgc
    43081 ataagaggct gaacgatcca ccttagtcgc atctttacca gagaaggcac caccaccatg
    43141 acgagagtag ccaccataag tatctacaat aatcttacga ccagtcaaac ctgagtcccc
    43201 ttgaggacca ccgattacaa aacgacctgt cggattgata aagaattttg tcttatcatc
    43261 aagataagaa gatggaataa cttctttgat gaccttgtca atcacatctt gatggatttg
    43321 ttcattagtg gcctctggat catgctgagt agaaataacg actgtatcta cacgtaccgg
    43381 acggtcattt tcatcgtact caactgtaac ttgtgatttt gcatctggac ggagatagct
    43441 aatttctcca gacttacgaa gttctgccag acgacgaacc aatttatgac tgagtgcaat
    43501 tggcaatggc atgagctctt ctgtttcatc tactgcaaat ccaaacatga gcccttggtc
    43561 acctgctcca atcaagtcca gtggatcttg atcagcattt ccacgaacct ccaaggcttc
    43621 gttaacacct tgagcgatgt caggagattg ttccaccaaa gatgggtgta ctcccaccgt
    43681 ctcagcagaa aatccatatt ctgtattggt ataaccaatc tctgcaatgg tatcacgaac
    43741 cacacggtta atatccacat aggcatttgt agaaatttca ccaaaaacgt ggacagaacc
    43801 agtatataca gctgtttcag cagcaacgtg cgcctctgga tcctttgcta aaatagcatc
    43861 caaaatcgca tctgaaattt ggtctgcaat cttatccgga tgcccctcag atacagattc
    43921 agacgtgaat aatttacgct ctgacataaa aatgtccccc cttaaaaaat attgttatag
    43981 agttacaaag tactgatagg aaaactcttc gtaataaata cctaccatct tatcgggact
    44041 atataacttt atcattatac catttttaat cgcaatttgc agtctttaat gtagtgtttc
    44101 ggtttagata gttcgttctc ataaagcaaa aagttggagc tcaatactcc aacaatttct
    44161 attaaaatgt ttggcgttta gctttacgct ctgcacgacc gcgagcgcga ttttcagcac
    44221 gcttggtttt acggcgcttt tcatcaaccg cccattgaat tttcttctta taacccggtt
    44281 tgactttttt ctttttcttt ttaaccaaac caatcatttc gatatcaagt ttatcttgtt
    44341 ttttctcacg gttggcacga cgatcacggt cataggtatc ttgaaattcc ccgtctttga
    44401 ccatcttagg actaaacttg attcccaatt tctccaactc acggatatcc gagtcatcac
    44461 ttggctgata aagggtaata gctgtacctg gtaggccatt tcgtccagta cgaccaacac
    44521 gatgaacaaa aaaagataag tcttgcggaa tggcatcatt gatgacatgg ctgacacctt
    44581 caatgtcaat cccacgcgct gctaaatctg ttgcgacaat atactcaaaa tccagatttt
    44641 gcacctgatt catgattcgc ttgcgttcac gaggggcaat atctccatgg atttttgcaa
    44701 ccttcaagcc ttgagcagtc agatatgaat gcaattcatc agcacgcgtt ttagtgttaa
    44761 caaaaatcat tgccaaatac ggctgcatca actgagtcaa ctggtaaatt tgagcattct
    44821 tatcatgtcc cttggtcgaa atcaaccaat tatcaatggt gtcagaaata accgttttgg
    44881 tcttaatttt ctccataaca ggattggata agtatttttt caagaatggt tgcagttttt
    44941 gtgggatagt cgctgagaag accatgaatt gcaagtcttt tggaagactg ccagcaatct
    45001 tatcaacagt ttccaagaat cccatatcca aggtcatatc tgcttcatca acaacaaatg
    45061 tcttggcttt atgaatagct aaatcaccag atttaaccaa gtcgtagata cggcctggtg
    45121 ttccaataac aatatgaggc tgattgcttg ccaatttctc aatctggcga gccttatccg
    45181 taccacccac ataattaacc acacgaactt cgacatctga gtgagctgaa atctgacgcg
    45241 ctacttggta aatttgagta gccaactcac gactcggtgc agtaatcact gcttgtacac
    45301 tatcgctagc ttcatctaat tgctggaaaa tcggtaacaa gaaagtatga gtcttacctg
    45361 aacctgtttt tgattctcct actaggtcac gacctgccaa aacaatagga atcaacttgt
    45421 cttgcacctc tgttggagtt gtaaatttta actccttcaa ggcttctcta atatagtttt
    45481 taaattgaaa tttcgtaaat gacataacat cctcgattct atctatctta tcaattatac
    45541 catattttga acattttcag tagtccgact catctgacct atcacaaggc tctcaatttc
    45601 tctaaatata gtaaaatgaa ataagaatag gatgaatcga tcaggacagt caaatcgatt
    45661 tctaacaatg ttttagaagc agaggtgtac tattctagtt tcaatctact atacctctct
    45721 tatactaaaa atcatacatt tgctttcttt ccaaaatgca aaaagagaag accaaattgg
    45781 tcttctctca gccttaaagc ttgcgactca gatagatttg tcaaacagtc aaacagttgc
    45841 ttaagaatct tgtaaaagtc gaaagcttct agaaaaatta gtacttaatc atctttccgc
    45901 ttgcgaccaa gaagcaacac tgctagacct gcaagaagag agctagtcac tagtccagtg
    45961 agtgaaagtg gtgcagtact acccgcattt ggcaatgtag cttgtttttc cttttctcct
    46021 gctggacctt gagctggtgg attctcttca ggttttcttg gttgttctga ttgatgtcta
    46081 tctgcctgat tattatcagt tccctgctct ccccctggat tcactggtgg aactggattt
    46141 ggttgaggtg ctggttgtgg cgttggtgcc ggctgtggtt gtggactagg atcttgctta
    46201 gcttcaaaga cccacttacc gacgaaggtt agatttgacc cctcttccac ttcttggtaa
    46261 cctaagaatg tccatgtacc atttgcaaca gcaacttcta caggcatcgg tgcttctgga
    46321 acgaaagcaa tcgctacaga agaacttgta gtatatgttc cactttgtgg gagtaagctc
    46381 aatacttctg ctggcaagtt gttgtctgcg gttgcact
//
LOCUS       DAVKWZ010000009        45736 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000009 DAVKWZ010000000
VERSION     DAVKWZ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 45736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45736
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.009"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            102..416
                     /locus_tag="VW854_000572"
     CDS             102..416
                     /locus_tag="VW854_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEV0261856.1"
                     /translation="MAKALIICAAGMSSSLMAQKTTEFLKGKGKDIVVDAISATEGGK
                     AIAAAAFDLYLVSPQTKMYFKQFEEAGSKAGKPVVQIPPQAYIPIPMGIEKMANLILE
                     NI"
     gene            534..2513
                     /locus_tag="VW854_000573"
     CDS             534..2513
                     /locus_tag="VW854_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEV0261857.1"
                     /translation="MNVLNIKEKDILQFLIKNKERFVTSKELAEYLSCSDRTVRNVLK
                     LIEKTMIIQGVRLISKQGQGYQIFFENQGAYQEFRQTYELEEDYTKTAVSKGDDRLVF
                     ILNKLLFEQVPVLFDDLADELYVSRSTLSHDFRKIRVMLSEYNLSIESRANKGVYVSG
                     EERDKRRFIINYFLENQFFKTIHCYVKFNFFDQTVPLEEFARIVLDECQEANLKLSDF
                     VLQNLVVHIALSVIRLKSGFEIKNIDCQMTDDAIERKVAQRILSKVREVTNQEFPVQE
                     IDYITLHLLAKSQQCQKNQKNISEEVLKKSLFKTFQNLGLDDMYNFSSDFQLIEGLIT
                     HLMTLQVRLESRITLNNPLVDEIKQNYSDIFFMTREILANMDKFLEWTISDDEVAYVS
                     LHFLAAMERSKESTKFNILAICATGFGAAQMLRNRLETEFGKRVEVVDVIGYYELNQE
                     KLKGIDFIVSAVDLSNLYFQIPVFKVSVFLKSDEMEMIRKAMDQMQVSSYVQSSKINK
                     FENNGFRQYFSKENFLICTESDKVNLLEKMVEGLSVGESNEFEQSLLYGIKQREELSS
                     VVFSEKIAVPHPIQPFGTEGKVSVAICKDSLLWDNQSSHVQLVFLLSPSIYGNEGLAT
                     VTKKIVSLTENDELQNQLISCNNFEDFINIFEKIK"
     gene            2523..2837
                     /locus_tag="VW854_000574"
     CDS             2523..2837
                     /locus_tag="VW854_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEV0261858.1"
                     /translation="MNQEELQVAAFEIILHSGNARSEIHEAFAKMREGSFDDAESKLN
                     QSNEIILEAHHAQTKLLQEYASGVEIKIEIIMVHAQDHLMTTMTLLEVAKEMLALYKK
                     VN"
     gene            2868..3374
                     /locus_tag="VW854_000575"
     CDS             2868..3374
                     /locus_tag="VW854_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261859.1"
                     /translation="MDKKERQKIEQQRREMALTNTFFNRYLLLRYSIALFFFGNIYWL
                     LNQFINPSPIIIFPIMLIVFSILATIEQFKLYGNRKEKLGITLMFVRIQMLISIGLLV
                     LTWTSWFKNLFPIFENNQVARLFVFVVLLLGLVLSLLDIRRIKKIYKRTDKVYQQFVQ
                     LEKNSLSL"
     gene            3454..4800
                     /gene="celB"
                     /locus_tag="VW854_000576"
     CDS             3454..4800
                     /gene="celB"
                     /locus_tag="VW854_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEV0261860.1"
                     /translation="MDQQNGLFGFLENHVMGPMGKLAQFKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVADIFSASFDKFRSLYMVANYATMGSLSLYFVLSLAYEL
                     TKIYAEEEELNMNPLNGALLALMAFVMTVPQIIFDGGMMKTVTSLKEGAVIADGWAMG
                     NGVARFGTTGIFTAIIMAIVTVLIYRMCVKHNWVIKMPEAVPEGVSRGFTALVPGFVV
                     AFVVIFINGLLVAMGTDIFKVIAIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGAN
                     IVFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLGLCLYIAFASKSEQL
                     KAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPI
                     IAQVPWPTPVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI"
     gene            5179..5355
                     /locus_tag="VW854_000577"
     CDS             5179..5355
                     /locus_tag="VW854_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261861.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            5757..7970
                     /locus_tag="VW854_000578"
     CDS             5757..7970
                     /locus_tag="VW854_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HEV0261862.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSQTGSFEDRTTQLIYNRDFGQ
                     ISLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVQTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHFFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNMSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(8192..8668)
                     /locus_tag="VW854_000579"
     CDS             complement(8192..8668)
                     /locus_tag="VW854_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEV0261863.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRSKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDHKSGPLGKRIEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            8891..12094
                     /locus_tag="VW854_000580"
     CDS             8891..12094
                     /locus_tag="VW854_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HEV0261864.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSSWTGSKAQGWSAWVDQKNSSADASTRVIEAKDG
                     AITISSHEKLRAALHRMVPIEVKKKYKLRFKIKTDNKIGIAKVRIIEESGKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVAAQLSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKE
                     VKKIPLKILASVKDTYTDRLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRTYLWEKFSNYKTSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSME
                     WMHKHVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYHWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGF
                     GLSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDS
                     KKNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYM
                     LIPNVGRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVL
                     KRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            12474..12850
                     /gene="tnpA"
                     /locus_tag="VW854_000581"
                     /pseudo
     CDS             12474..12850
                     /gene="tnpA"
                     /locus_tag="VW854_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148688.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(13237..13866)
                     /locus_tag="VW854_000582"
     CDS             complement(13237..13866)
                     /locus_tag="VW854_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEV0261865.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNKKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSALSPAYISAIKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYVTLR"
     gene            complement(13876..14877)
                     /locus_tag="VW854_000583"
     CDS             complement(13876..14877)
                     /locus_tag="VW854_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEV0261866.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITVAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEELFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(14908..15549)
                     /locus_tag="VW854_000584"
     CDS             complement(14908..15549)
                     /locus_tag="VW854_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEV0261867.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKDYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(15568..16383)
                     /locus_tag="VW854_000585"
     CDS             complement(15568..16383)
                     /locus_tag="VW854_000585"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEV0261868.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLIGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
     gene            16653..17087
                     /locus_tag="VW854_000586"
     CDS             16653..17087
                     /locus_tag="VW854_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV0261869.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            17099..18289
                     /locus_tag="VW854_000587"
     CDS             17099..18289
                     /locus_tag="VW854_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="unsaturated chondroitin disaccharide hydrolase"
                     /protein_id="HEV0261870.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            18300..18791
                     /locus_tag="VW854_000588"
     CDS             18300..18791
                     /locus_tag="VW854_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV0261871.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNATGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            18806..19585
                     /locus_tag="VW854_000589"
     CDS             18806..19585
                     /locus_tag="VW854_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV0261872.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            19572..20390
                     /locus_tag="VW854_000590"
     CDS             19572..20390
                     /locus_tag="VW854_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV0261873.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGENQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            20390..20683
                     /locus_tag="VW854_000591"
     CDS             20390..20683
                     /locus_tag="VW854_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV0261874.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIQDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            20705..22606
                     /locus_tag="VW854_000592"
     CDS             20705..22606
                     /locus_tag="VW854_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEV0261875.1"
                     /translation="MKEERRQFFERIDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWAYMLNRQEYLLQFMIGYVVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSHYRAKYTLSNWGILQTIPMLAIYHFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEMLSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     KRAWQPKFMSFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGMHY
                     IEGAYWSAEPDLPYLHRRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSENIGVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            22666..23667
                     /locus_tag="VW854_000593"
     CDS             22666..23667
                     /locus_tag="VW854_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV0261876.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(23777..24112)
                     /locus_tag="VW854_000594"
     CDS             complement(23777..24112)
                     /locus_tag="VW854_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261877.1"
                     /translation="MKSLARLLIIHVFISIFLFFALTSGAISHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(24123..24365)
                     /locus_tag="VW854_000595"
     CDS             complement(24123..24365)
                     /locus_tag="VW854_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261878.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            complement(24376..24918)
                     /locus_tag="VW854_000596"
     CDS             complement(24376..24918)
                     /locus_tag="VW854_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEV0261879.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADSIGNQVFIVLFYLM
                     IFGNIIPLLLAYKYPQEVALIYPPLILVIALIASGYVTYQMKKTGITVIEPDMLNEKE
                     SKQLHYPGLKAGLFFGLWMFFITPLLSILIDEGQDYFHSLLTIRNGVSSILGSIFFGA
                     SIQFLISRRIAKTKKNQDED"
     gene            complement(24934..25128)
                     /locus_tag="VW854_000597"
     CDS             complement(24934..25128)
                     /locus_tag="VW854_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0261880.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEDDF"
     gene            25294..26244
                     /gene="rsmH"
                     /locus_tag="VW854_000598"
     CDS             25294..26244
                     /gene="rsmH"
                     /locus_tag="VW854_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEV0261881.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            26256..26573
                     /gene="ftsL"
                     /locus_tag="VW854_000599"
     CDS             26256..26573
                     /gene="ftsL"
                     /locus_tag="VW854_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEV0261882.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            26577..28829
                     /gene="pbpX"
                     /locus_tag="VW854_000600"
     CDS             26577..28829
                     /gene="pbpX"
                     /locus_tag="VW854_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HEV0261883.1"
                     /translation="MKWTKRVIRYVTKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGVT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKAEEVPDMYGWTKETAETLAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            28831..29811
                     /locus_tag="VW854_000601"
     CDS             28831..29811
                     /locus_tag="VW854_000601"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEV0261884.1"
                     /translation="MFISISAGIVTFLLTLVEIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDILSVFGYPVHLGFFYIFFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            29951..30139
                     /locus_tag="VW854_000602"
     CDS             29951..30139
                     /locus_tag="VW854_000602"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261885.1"
                     /translation="MLEIDLTVLIDLSCSYFILLYSFEMVDRTDEVSSKHGFEVVDKE
                     KLMWFEEVFEECKKILVS"
     gene            30420..32525
                     /locus_tag="VW854_000603"
     CDS             30420..32525
                     /locus_tag="VW854_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV0261886.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRRLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELERKIENHTEDMKVTASVNDVAESVERMIG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKA"
     gene            complement(32822..33304)
                     /locus_tag="VW854_000604"
     CDS             complement(32822..33304)
                     /locus_tag="VW854_000604"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEV0261887.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(33399..34883)
                     /locus_tag="VW854_000605"
     CDS             complement(33399..34883)
                     /locus_tag="VW854_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEV0261888.1"
                     /translation="MKKQAFSSEQYLNLQRDHILDRINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQLAADAFRNQLEKNRIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTILDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLDINVTFDPYYQYDRLYRK"
     gene            35036..36646
                     /locus_tag="VW854_000606"
     CDS             35036..36646
                     /locus_tag="VW854_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEV0261889.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNEDGIGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIAAIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENTDSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKELLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEEINVQEALANSDSIFYTYQKL
                     IQIRKENSWLIRADFKLLDTADKVFAYIRKDGDRRFLVVANLSNDKQNFSVDGKVRSV
                     LIENTAAKEVLEKQVLTPWDAFCVEMTD"
     gene            complement(36724..37558)
                     /locus_tag="VW854_000607"
                     /pseudo
     CDS             complement(36724..37558)
                     /locus_tag="VW854_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547411.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(37506..38891)
                     /locus_tag="VW854_000608"
                     /pseudo
     CDS             complement(37506..38891)
                     /locus_tag="VW854_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677226.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
     gene            39067..40512
                     /locus_tag="VW854_000609"
     CDS             39067..40512
                     /locus_tag="VW854_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV0261890.1"
                     /translation="MSRRFKKSGSQKVKRSVNIVLLTIYLLLVCFLLFLIFKYNILAF
                     RYFNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSDIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNIDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDIHYYVRLNFTSFLKLIDLLGGVDVYNDQ
                     DFTSLHGKFHFPVGNVHLDSEQALGFVRERYSLADGDHDRGRNQQKVIAAILQKLTSS
                     EALKNYSMIIDSLQDSIQTNMPLETMINLVNAQLESGGTYKVNSQDLKGRGRTDLPSY
                     AMPDSNLYMMEINDSSLASVKTAIQDVLEGR"
     gene            40514..41245
                     /locus_tag="VW854_000610"
     CDS             40514..41245
                     /locus_tag="VW854_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEV0261891.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLTEAYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVASDLVIAYGAEIYYTPDVLGKLEKNRIPTLNNS
                     RYALIEFSMNTPYRDIHSALIKILMLGITPVIAHIERYDALENNEKRVRELINMGCYT
                     QVNSSHVLKSKLFGEPYKFMKKRAQYFLERDLVHVIASDMHNVDSRPPHMAEAYDLVS
                     QKYGETKAQDLFIDNPRKIVMDQLI"
     gene            41254..41946
                     /locus_tag="VW854_000611"
     CDS             41254..41946
                     /locus_tag="VW854_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein CpsC"
                     /protein_id="HEV0261892.1"
                     /translation="MKEQNTIEIDVFQLLKTLWKHKLIILLVALVTGAGAFAYSIFIV
                     KPEYTSTTRIYVVNRNQENKPGLTNQDLQAGTYLVKDYHEIILSQDVLEKVATNLKLD
                     IPVKTLTSKVQVTVPADTRIVSISVKDKQPEEASRIANSIREVAAEKIIAVTRVSDVT
                     TLEEARPATTPSSPNVRRNTLVGFLGAAAVTVITVLLIELFDTRVKRPEEVEDVLQMP
                     LLGVVPDFNKMK"
     gene            41956..42654
                     /locus_tag="VW854_000612"
     CDS             41956..42654
                     /locus_tag="VW854_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HEV0261893.1"
                     /translation="MPTLEISQAKLELAKKTEEYYNALCTNLQLSGDDLKVFSISSVK
                     AGEGKTTTSTNIAWAFAHAGYKTLLIDADMRNSVMSGVFKSRERITGLTEFLSGTTDL
                     SQGLCDTNVENLFVIQAGSVSPNPIALLQSKNFSTMLGTLRKYFDYIVVDTAPIGIVI
                     DAAIIMQKCDASILVTKAGETKRRELQKAKEQLEQTGKSCLGVVLNKFDTSVDKYGFY
                     GSYGSYGSYRKQKK"
     gene            42669..44030
                     /locus_tag="VW854_000613"
     CDS             42669..44030
                     /locus_tag="VW854_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV0261894.1"
                     /translation="MNEKLAKSSVAIVQSFLVILLTYLLSAVRETEIVSTTAIVLYIL
                     HYFVFYISDYGRNFFKRRYLIELVQTLKYILFFALAISISNFFLEDRFSISRRGMIYF
                     LLLHVFLVYMLNLFIKWYWKRAYPNFKGSKKVFLLTATSHVEKVLDRLIESDDVVGEL
                     VAVSVLDKPDFQHDDLKVVAEGEIVNFATREVVDEVFINLPSEKYNIGELVSQFETMG
                     IDVTVNLNAFDWARNKQICEMAGLNVVTFSTTFYKTSHVIAKRVIDIIGSLVGLILCG
                     LVSIVLVPLIRKDGGSAIFAQTRIGKNGRHFTFYKFRSMCVDAEDKKRELMEQNTMQG
                     GMFKVDDDPRITKIGHFIRKTSLDELPQFYNVLKGDMSLVGTRPPTVDEYEHYTPEQK
                     RRLSFKPGITGLWQVSGRSEIKNFDEVVKLDVVYIDGWTIWKDIEILLKTVKVVLMKD
                     GAK"
     gene            44062..45240
                     /locus_tag="VW854_000614"
     CDS             44062..45240
                     /locus_tag="VW854_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEV0261895.1"
                     /translation="MKKSVYIIGSKGIPAKYGGFETFVEKLTEYQKDGNIQYYVACMR
                     ENSAKSGFTADTFEYNDAICYNIDVPNIGPARAIAYDIAAVNKAIEIAKKNKDEAPIF
                     YILACRIGPFIARLKKKIQAIGGTLFVNPDGHEWLRAKWSLPVRKYWKFSEQLMVKYA
                     DLLVCDSKNIEKYIQNDYKQYQPKTTYIAYGTDTSPSILKSEDLKIRSWYQEKGLSEN
                     GYYLVVGRFVPENNYETMIREFIKSKSKKDFVLITNVEQNKFYDQLLQETGFDKDPRV
                     KFVGTVYDQELLKYIRENAFAYFHGHEVGGTNPSLLEALASTKLNLLLDVGFNREVGE
                     DGAIYWRKDNLHKVIEESEQKTIEEIKEIDILSTEQVEKRFTWDFIVNEYENLFLLGK
                     "
BASE COUNT        14096 a         7803 c         9981 g        13856 t
ORIGIN      
        1 gggaattgtc attggaaacg ctttatttta taataaaaat agataacaaa taagccaact
       61 tagcacagct ttgattggat ttaagaaaag gagaaaatat tatggcaaaa gctttaatta
      121 tttgtgcagc aggaatgtct tcatcactta tggcacagaa aacaacagag tttctaaaag
      181 gaaaagggaa agatattgta gttgatgcta ttagtgctac tgaaggaggg aaagctatcg
      241 cagcagcagc ttttgatttg tatcttgtca gccctcaaac aaaaatgtac tttaagcaat
      301 ttgaagaggc tggttctaaa gcaggaaaac cagttgtaca aatacctcct caagcatata
      361 ttcctattcc aatgggaatt gaaaaaatgg ctaatttgat tcttgagaat atttgatgaa
      421 cacatgaaat gtcaataaat atttaggtta agataaattg ttttgagttt gatataaact
      481 gatttaagtg tataatatgc ttcaatatat gaaaacatac gttttaggag ggaatgaatg
      541 tgttaaacat taaagagaaa gatatccttc aatttttaat aaaaaataag gaacgttttg
      601 ttacaagcaa agaactggca gaatatttat cctgttcaga tagaactgtc cgtaatgtac
      661 tgaaattaat tgagaagaca atgattattc aaggagtccg gttgatttca aaacaaggtc
      721 aagggtatca aatatttttt gagaatcaag gcgcttacca ggagtttaga caaacttatg
      781 aactggaaga agactatacg aaaactgcgg tttcaaaagg tgatgatcgt ttagtattta
      841 tcttaaataa attattattt gagcaagttc cagttctttt tgatgattta gctgatgaac
      901 tttacgttag tcgttcaaca ttatctcatg atttcagaaa gatacgagtg atgttatcag
      961 agtataattt gtccattgag agtagggcga ataaaggagt ttatgtctca ggcgaggaac
     1021 gtgataaacg acgttttatc ataaattatt tcctagagaa tcaatttttt aaaacaatac
     1081 attgctatgt aaaatttaac ttttttgatc aaactgttcc attagaagaa tttgctagaa
     1141 ttgttttgga tgaatgccaa gaggccaacc taaaactatc tgattttgta ctacagaatt
     1201 tagtagttca catagcattg tctgtgattc gtttaaaatc cggatttgaa ataaaaaata
     1261 tagactgtca gatgactgat gatgcaatag agagaaaggt tgcccaaaga attctttcta
     1321 aagtaagaga agtaacaaat caggaatttc cagttcagga aatagactac attactttgc
     1381 atttgttagc taagagtcaa caatgtcaaa aaaatcagaa gaatatttct gaagaagttt
     1441 taaaaaaatc tttatttaaa acatttcaga atttaggatt agatgatatg tataactttt
     1501 cttcagattt ccaactaatt gaaggattga taactcatct aatgacacta caagtacgtt
     1561 tagaaagccg gattacctta aataaccctt tggtagatga aattaaacag aattatagtg
     1621 atatattctt tatgactaga gaaattcttg caaatatgga caagttcttg gagtggacaa
     1681 tatcagatga tgaagtagca tatgtaagtc ttcacttttt agctgctatg gaacgtagca
     1741 aagagagtac taagtttaat attcttgcta tttgtgcaac aggatttggt gcagcccaaa
     1801 tgttaagaaa tcgtttggaa acggagtttg ggaagcgagt agaagttgtt gatgtaatag
     1861 gctattatga attgaatcaa gaaaaactta aaggaataga ttttatagtg tcagctgttg
     1921 atttatcaaa tctatatttt caaatcccag tttttaaagt tagtgtgttt cttaagagtg
     1981 atgagatgga aatgattaga aaagctatgg atcagatgca agtttctagt tatgtacaat
     2041 catcaaagat taacaaattt gaaaacaacg gctttagaca gtattttagt aaagaaaatt
     2101 ttttaatatg tactgaatca gacaaggtaa atttacttga aaagatggtt gagggtttat
     2161 ctgttggtga atcaaatgag tttgaacaat ctttacttta tggaattaaa cagcgcgaag
     2221 aattaagttc agttgttttt tctgaaaaaa ttgcagttcc gcatcctatt cagccctttg
     2281 gtacagaggg aaaggtttcg gttgcaatct gtaaagattc gttactctgg gataatcaaa
     2341 gttcacatgt ccagctagta tttcttctat ctccatcaat atatgggaac gaaggtttgg
     2401 caactgtgac taaaaagata gtctctctga cagagaatga tgaattgcaa aatcagctaa
     2461 tatcttgcaa taactttgaa gattttatta acatatttga gaagataaaa taggaggttc
     2521 atatgaatca agaggaactg caggttgcag catttgaaat tatcctgcat tcgggaaatg
     2581 caaggtcaga aatacatgaa gcatttgcaa aaatgagaga aggtagtttt gatgatgcag
     2641 aaagtaaatt aaatcaatca aatgagataa ttcttgaagc gcatcatgca caaaccaaac
     2701 tcttgcaaga atacgcaagt ggagtggaaa ttaaaattga aattatcatg gtacatgctc
     2761 aagatcattt aatgaccact atgacattat tagaagtagc aaaggaaatg ttagctcttt
     2821 ataagaaagt taactaaata tgtgttgtta tataaggagg aaatgatatg gataaaaaag
     2881 aacgacaaaa aattgaacaa caacgtcgag agatggctct aactaatact ttttttaatc
     2941 gatatcttct tctacgttat tcaatagcac tcttcttttt tgggaatatt tattggcttt
     3001 tgaatcagtt tattaatccc tcgcctatta ttattttccc cattatgctc atagtatttt
     3061 ctattttagc aactattgaa caatttaaac tttatggaaa taggaaagaa aagttgggaa
     3121 taacactaat gtttgttaga atacaaatgc ttatttcaat aggattactt gttttaactt
     3181 ggacaagttg gtttaagaat ctttttccga tttttgaaaa taaccaagta gcacgtttat
     3241 ttgtttttgt agttcttcta ttaggtttag ttctgagttt gttagacatt agaagaatta
     3301 aaaaaattta taaacgaaca gataaggttt atcaacaatt tgtccaattg gaaaagaact
     3361 cacttagttt ataatcctga tctagtataa tttgtatttg aaactagtct ttagaaagta
     3421 aaaattatta tgttttatta aggaggtcta tttatggatc aacaaaacgg gttgtttggt
     3481 tttcttgaaa accatgttat gggaccaatg ggcaaacttg ctcagtttaa agtagtacgt
     3541 gctatcacgg ctgcaggtat ggctgctgta ccatttacta ttgtaggatc aatgtttttg
     3601 gtattcagta ttttgccaca agctttctca ttttggccaa ttgtggcaga tattttctct
     3661 gcttcatttg ataaattcag atcactttac atggttgcaa actatgcgac tatgggttct
     3721 ctatctcttt atttcgttct atcacttgca tatgaattga caaaaattta tgcagaggaa
     3781 gaagaactca atatgaatcc tcttaatggt gccttgcttg ccttgatggc ttttgtcatg
     3841 acagtaccgc aaatcatttt tgatggtgga atgatgaaga ctgtgacaag tctaaaagaa
     3901 ggtgcagtaa ttgcagatgg atgggcaatg ggaaatggag tcgcacgttt tgggacaaca
     3961 gggattttta ccgcaatcat tatggcaatt gtgactgttc ttatttatcg tatgtgtgtt
     4021 aaacataatt gggttattaa aatgcctgaa gctgttccag aaggagtttc tcgtggattt
     4081 accgctttgg ttccgggatt tgttgttgca tttgttgtta tctttatcaa cggtcttctt
     4141 gtagcaatgg gaacagatat ttttaaagtc attgcaattc catttggttt tgtatccaat
     4201 ctgactaatt cgtggattgg tttaatgatt atttatctat tgactcaact actttggatt
     4261 gtaggtatcc acggtgcgaa cattgttttt gcatttgtta gtccaattgc tcttgccaac
     4321 atggctgaaa atgctgctgg cgggcacttc gctgttgcag gtgaattttc taatatgttt
     4381 gtaattgcag gtggttctgg tgcaacttta ggactatgtt tatatattgc ttttgcctct
     4441 aaatctgaac agcttaaagc aataggacga gcatctgtag ttccagcctt atttaatatt
     4501 aatgaaccat taatttttgg attacctatt atctataatc cagccttggc tataccattt
     4561 attttagcac caatggttac tgctactatt tattacgtag cgaattctct aaactttatt
     4621 aagccaatta tcgcacaggt tccatggcca actccagtag ggattggagc tttcttaggg
     4681 acagcagatc ttcgagctgt attagttgct ctagtatgtg catttgcagc attcctagtc
     4741 tatcttccat tcatccgtgt atatgatcaa aaattggtga aagaagagca aggtatctaa
     4801 taagtaaaaa agtatactcg atgatagaat ccatatagtt taaataatga gcgttaacat
     4861 tagcagtgaa gaggtctttt taagatattc ttctctgttt tttcatattt tttcgctatg
     4921 tagggtatga agcaaaatag aaagtagttc gaatagttga taaacaggga actgctgtta
     4981 tttaaaaaaa caataaagta aagtatgtta tcgtagaata cgatacaata gtaaaaaagc
     5041 aaaaagaatg cgttttatat ttgggtttga ggtttataaa ataggataag cgtgctgaaa
     5101 acttagatat gacaaacaaa ataaaatttt taaaagctac agagaaacat ggtataatgt
     5161 atctaaagga ggtagcatat gtccttagat atagataaag aaaagatgac aatcatggga
     5221 atagcttttg aaaaccgatc cgtttttaag agtgtttggt atgctttaag tacaaatatg
     5281 attgaaggat ggcgacctac ggttagtgat gttgaaaaat tacgagatga agctcttgct
     5341 ctggggatga cttaatgaag cgcaactgtt acgagtctta tgactatgtt gatccggata
     5401 atttatatac cgatccgggt tcttcagttc taagaaataa gcaggaagga aaaaagcacg
     5461 tgagcttgaa tatcgtatga ttgctagtaa gagtttgaaa ttatttatca atcctatctt
     5521 agttaatagt ttaaaagtag tttgtttcac aactgcataa gcttagaaaa gttgaaaagt
     5581 gataactctt taataaactc ttggtttgtt aaaagatatt ttgtccacta catttttttc
     5641 aattgactat tatttgtcat agatataaca gatagaacat tgcattcaga ataatgaaaa
     5701 acgcatcaaa aatggtgcgt tctgttttta tgctaccata aaaggaggaa aacaaattga
     5761 aaatttttaa gggagagttt tatcgaatct ctgtattaac agacaagcta gtaaggttag
     5821 aatactctca aactggaagt tttgaggata gaacgacaca acttatctat aatagagatt
     5881 ttggccaaat ttcgttagat tatatcgaga catcaaacgt actagatatt atgacggact
     5941 attttcatct gcactttaat aaaggagaat ttaacgccga aaatttattt atagaattaa
     6001 aaggaaattt tgccgtatat ggtagtcgct ggtattttgg tgaatctatt gaaacgttaa
     6061 aaggaacagc tcggactctg gataaggcag atggagcaat ctcgttagaa gatggaatta
     6121 ttagccgaaa tggtatagcc ttattggatg attctcaagg atttatttgg gatgaacaat
     6181 ctggttatat tgagagagaa aatcaaattg acctgtattt ctttgcctat gggcatgatt
     6241 atagaggagc aatcagagac ttttaccatt tgactggttc aacacccttg ttgccaagat
     6301 atgctttagg caattggtgg agtagatatt ggccttatac gtcggatgaa tacttggatt
     6361 taatagacag atttgaaaca gagaaaattc cattatctat cggtgtgtta gatatggatt
     6421 ggcatataac tgacattcca gctcgttttg gaagtggctg gacaggatat agttggaata
     6481 gaaacttaat accaaatcca gaacagttat tgcaacaact tcatgataga aagttaaaac
     6541 tctccttaaa tgtccatcct gctgatggga tacgggctta tgaagaagct tatcctcaaa
     6601 tcgcaaaacg attggggtta aatgtagaac tagaagaacc tgctattttt gattttttta
     6661 atccctcttt tagggaagcc tactttaaag atgttcatta tgagctagaa aagcagggag
     6721 tagatttttg gtggattgac tggcaacagg ggacacaagg catgctagat ccactttggc
     6781 ttttaaacca ttatcactat caggatagtt gtaaaaatgc agaaggtggt ttgattttat
     6841 caagatatgc aggtcctggt agtcaccgct accctgttgg tttttcaggg gatactatta
     6901 ttagttggaa ttccttaaga tttcaaccct attttacagc gacagcatct aatatcggtt
     6961 atagttggtg gagtcatgat atcggtggac atatgttggg ggattatgac gaagagctac
     7021 aaactagatg gctacagttt ggtgttttta gtccgattac tcgattacat agttctagaa
     7081 gtccttttaa tagtaaagaa ccttggttct tttcagaaac aacatctaag attatgaaga
     7141 aataccttcg tttgagacat cagatgattc cctatctata taccatgaat gtacagacac
     7201 atgaggaagg tgccccatta atcagtccaa tctattattt ctatccagag aatgatgaga
     7261 gctataatgt tccaaaccaa tacttttttg gaacagaact gatggtggct cccattgtag
     7321 aaaagatgga tttgacattc caatctgcaa aagtagatgt atggttccct gaaggtgaat
     7381 ggtatgactt cttttcagag aaaaaataca caggtggtgt gaagttaagt gtttataggg
     7441 acatctcgac tacgcctgtg tttgcaaaaa gtggtgcaat cattcccttg gttggttctg
     7501 agataggtat gggtgttgat ttacctgagg ttgtagattg gtatgtattc ccagggaaac
     7561 aacatttttt tgaaatgctt gaagatcaaa atggtcaaag atataaaaca agattatcaa
     7621 tcgattggga aatgggaatg gtagagttag cattacaagg agattctagt atcgttccaa
     7681 gcaatagaaa acatagaatt cattttaaag gaacgaatat gtctataatt gaattgccaa
     7741 ataagaatga tacagctaaa tttgaatgga aagataataa aaggacatct ttaaatgacg
     7801 aagtttttag actactaaag acagcttctc ttccatatga attaaaagat agattgttaa
     7861 atcaattcat caatgcgaaa aactctcatg acttaatgaa tatcttgcat catcaggata
     7921 aggaattgag agggcgtttg ttggaaatga tatttactag ccaaaactaa ttgaaataat
     7981 tagaaagtca tttgtttagt gtatatgaag attctgtatg gtattcgtta tcactggtca
     8041 cattgaaaat gtgaaataag cacctacaac atagacttta caacacttca tgtttcatat
     8101 catggggtgt ttttctattt aagtcaaagc ccatcaaaaa atctcactat tcatcaggtt
     8161 aaaggaaacc taatcatagt gagattgtga attatagtag agtttgtatc gcctcttcaa
     8221 tttgttttgg gtctgttttt gaagaaaagc gttcaaagac ttgcccatca cgaccaatga
     8281 gaaacttagc gaaattccat tcgattcgtt ttcctagtgg gccagattta tggtctttta
     8341 accagacata gagagggtct gcttccttac cgttgacctt aatcttggca aaacgtggga
     8401 aggtggtttg aaaatgtagg ctacagaagg cgttgatttc ctctgcgctg ccgggtgctt
     8461 gtcccataaa ctgattgcaa gggaaatcca atatttcgaa gccctgatct tgatagcgtt
     8521 catagagttc ttgaagtccc tggtactggg gcgttaaacc acatccagta gcagtgttga
     8581 caatcaagag aactttgcta cgatagctat ccaagggagt tgcttggttg ttttggttca
     8641 aaacggaaaa atcataaagt gaagtcatga gggcctcttt tctttttcta cattgtaaca
     8701 gtttttactt ttttcgtcga gtcggatgga agatattgtt gaagaataag ccctcaatat
     8761 gatagaaatc gtttacaaat aactttaaaa atggtatgat ttaactaaac cggtttactt
     8821 agttgctata aatctctaat ttgagattta gttcctattg attttacaat atgtttattg
     8881 gagtgtatac atgcaaacaa aaacaaagaa gctcattgtg agtttgtctt cacttgtttt
     8941 atcaggattt ttattaaacc attatatgac agttggagcg gaagaaacga ctacgaatac
     9001 cattcagcaa agccagaagg aagttcagta tcagcaaagg gatacaaaaa atttagttga
     9061 aaatggtgat tttggtcaga cggaggacgg aagcagttcg tggacaggaa gcaaagctca
     9121 ggggtggtca gcttgggtag accagaagaa tagttctgca gatgcctcaa ctcgagtcat
     9181 tgaggctaag gatggggcta tcactatctc aagccatgag aaattaaggg cagcgcttca
     9241 ccgtatggtt cctattgaag ttaagaaaaa gtataaactg cgtttcaaga ttaaaacaga
     9301 taataaaatc gggattgcca aagttcgtat cattgaggaa agtggtaagg acaagcgatt
     9361 gtggaattct gcaacgacgt caggaacaaa ggactggcag accattgaag cagactatag
     9421 cccgacttta gatgttgata aaatcaagct ggagttattc tatgaaacag gaactgggac
     9481 tgtttccttt aaggatattg agctggtaga ggtagcagcc cagctttctg aggattctca
     9541 aacagataaa cagcttgagg aaaagattga tttaccaatt ggaaaaaaac atgttttttc
     9601 tcttgcggac tatacttata aggtagaaaa tcctgacgtt gcttcagtca aaaatggaat
     9661 tttagaacct cttaaggaag ggacaaccaa tgtcattgtc agtaaagatg gcaaggaagt
     9721 gaaaaagatt cctttgaaga ttctagcctc tgttaaggat acatacacag accgtttgga
     9781 tgactggaat ggcatcatcg ctgggaatca atactatgat tctaaaaatg aacagatggc
     9841 caaattaaac caggaattgg aaggaaaggt agctgatagc ctatccagta tttcaagtca
     9901 ggcggaccgc acctatttgt gggaaaaatt ttcaaattat aaaacgtctg caaatctgac
     9961 tgccacttat cggaaattgg aggagatggc caagcaagtg accaatcctt cttctcgtta
    10021 ttatcaagat gaaactgtcg ttcgaacagt cagggattcc atggaatgga tgcataaaca
    10081 tgtctacaat agtgaaaaga gcattgttgg gaactggtgg gattatgaaa tcggtacacc
    10141 tcgtgccatc aacaatacct tgtctctgat gaaagaatac ttctctgatg aggaaattaa
    10201 aaaatataca gatgtgattg aaaaatttgt accagatccc gaacatttcc gaaagacgac
    10261 tgataaccca tttaaggctc taggtggaaa cttagttgat atgggaagag taaaagtaat
    10321 agctggttta ctgcgtaagg atgatcaaga aatttcttct accattcgct cgattgagca
    10381 agtgttcaag ttggtagacc aaggtgaagg tttttatcaa gatggatcct atatcgacca
    10441 taccaatgtt gcctatacgg gtgcttatgg gaatgttttg attgatggcc tgtctcaact
    10501 gttgccagtc attcaaaaga ccaagaatcc aatcgataaa gataaaatgc aaaccatgta
    10561 ccactggatt gataaatcgt ttgctccttt gctggtgaat ggagagctga tggatatgag
    10621 tcgtggacgc tcgatcagtc gtgcaaatag cgaggggcac gtggccgcag tagaagtact
    10681 aagagggatt caccgaatag cggatatgtc tgaaggagaa accaaacaac gtttgcagag
    10741 tcttgtgaag accattgttc aatcggatag ttattatgat gtctttaaga atttgaagac
    10801 ttataaggat atcagtttga tgcaatcctt gttaagtgat gcaggagtcg caagtgttcc
    10861 aagaacaagt tacctatctg cctttaacaa gatggataaa acagccatgt acaatgcaga
    10921 gaaagggttt ggatttggct tgtcactctt ttccagtcgt accttgaatt acgaacacat
    10981 gaacaaggaa aataaacgtg gttggtatac gagtgatggg atgttctatc tttacaatgg
    11041 cgatttgagt cactatagcg atggctactg gccaacagtt aatccatata agatgcctgg
    11101 tacaacagag acggatgcta agagagcgga tagcgataca ggtaaagttt taccgtctgc
    11161 tttcgttgga acgagcaaac tagatgatgc caatgcgaca gcaaccatgg atttcaccaa
    11221 ctggaatcaa acattgactg ctcataagag ctggtttatg ctaaaggata agatcgcctt
    11281 tttaggaagc aatatccaaa acacttcaac agatactgct gcaactacaa ttgaccagag
    11341 aaaactggaa tcaagtaatc catataaagt ctatgtcaat gataaagaag cctcccttac
    11401 agaacaagaa aaggattatc ctgaaaccca aagtgtcttt ttagaatcat ccgattcgaa
    11461 aaagaatatt ggttactttt tctttaagaa gagttcaatc agtatgagta aggctttgca
    11521 aaagggagcc tggaaggata tcaatgaagg acagtcagac aaggaagttg aaaatgaatt
    11581 tcttacgatt agtcaggctc ataagcaaaa tggagattct tatggctata tgctcatccc
    11641 taacgtgggt cgtgccacct tcaatcaaat gataaaagag ttagaaagta gcctcatcga
    11701 aaataacgaa acccttcagt ctgtttatga tgccaaacaa ggagtttggg gcattgtgaa
    11761 atatgatgat tctgtctcta ctatttccaa ccaattccaa gttttgaaac gtggagtcta
    11821 taccattcga aaagaagggg atgaatataa gattgcctac tataatcctg aaacccagga
    11881 atcagctcca gatcaggaag tctttaaaaa gctagagcaa gcagctcagc cacaagtaca
    11941 gaattcaaaa gaaaaggaaa aatctgaaga ggaaaagaac cattcggatc aaaagaatct
    12001 ccctcagaca ggagaaggtc agtcaatctt ggcaagtcta gggttcttgc tacttggggc
    12061 gttttattta ttccgtagag gaaagaacaa ctaatttgtt catcatctag atgaattaca
    12121 tgaaactgtg gaaaatagaa aaaatccgaa gaaggccaaa tatcttcgga tttttctttt
    12181 aaaaaaggat aagttgatac ttatcctttt tacttcatta tgtgactata gcgttttgaa
    12241 actagaatag gacatcataa ctgctaaaag atttctataa attcatttga ttttcctaat
    12301 caatttattc gtatcctatt tcactctact atattagtcc gtctcgcacc tcacggagcg
    12361 agacaaaaca accacccgct atgcgggtgc gcgtcgaagg ttataccaaa aaaactccaa
    12421 acgcgataca ataaaggtgt tcaatccact tgtaaagcga aaggagaaaa aatatggcac
    12481 aaaaggctca tagtttatct cacacaaagt ggaggtgttc tatcacattg tgttcacccc
    12541 taagtataga cgaaaagtca tctataatca atatagaagt agtttaggcg aaatatttca
    12601 tcgcttgtgt agttataaag gtgttgaaat gatcgagggt cacttaatgc cagaccatgt
    12661 acacatgtta gtcagtattc cactaaggct aagtgtttcg agtttcatgg gttatttaaa
    12721 aggaaaaagt gcactcatga tgtttgacaa acacgccaat ctcaagtaca agtttgggaa
    12781 tcggcatttc tgggtggaag gttattatgt aagtacagta gagcttaata aagccacaat
    12841 taagaaatat agtcaagatt aaagaaatta tccagtggat gatttcttca tgagtatgaa
    12901 aatttgagaa cgagtaaagc atgatatata gtaaaatgaa ataagaacag aacaaatcga
    12961 tcaggacagt caaatcgatt tttaacaatg ttttagaagt agaggtgtac tattccagtt
    13021 tcaatctact atagcactag ataaattgag tgtaaaagaa tatgaggatc cctttaggga
    13081 tagtggtaag taataccaaa gtctcttaaa gaggcaagtg acgagtcaag agcaataagg
    13141 cttgaacaac gtgaaagcca gcgtctttag gcgctggctg gtaatttggg cttatagctc
    13201 tgggataaac cacccgtttg acgggtggtt atgattttat ctgagtgtaa catactgttg
    13261 ggcaatctcg ctgatgcggt caaagttgcc ttgggaagcg agtttattga gttcgccacc
    13321 aattccaacg gcgtctgcac cagcagcgaa ccattgaggg atgttgttta gaccgactcc
    13381 tccggttacc attacggaaa cttgtgggat cggtgccttg attgcagaga tatatgctgg
    13441 actgagagca ctacctggga agagtttgat gatttcacta ccggcttcaa gtgcagtcgt
    13501 gatctctgtg agggtaatac agcctggaat gtacggtgtg ctgtagagat tgcacatttt
    13561 cgcagtttca gcatggaaag atggagaaac aacgtaattt gctccggcta gaatggcatc
    13621 tctagcagtt acggcatcaa gcacagtacc tgcaccgata caaacactct gatcgtcctg
    13681 atacaagtct acaagttctt tgatgatttg tcctgcatac tgattggtat aggcgatttc
    13741 aatagctttg ataccgccct tgatacaagc aatcgaggct tgtagtcctt cttccttttt
    13801 atttcctcga atgacagcga caattttctg tttttttagt tcaataatcg tatctgattt
    13861 ggtcatgtaa ttctcctaac gaatgatatc ttgtgcattt gccagtaaat tttcaatact
    13921 agttgcggaa gtggagagat ggtctcctgg aagagtgcat ttgagagttg cgctcgcaac
    13981 tgcaaagtca atggtagttt ttagggagga atgatggagt agttggtaaa gggcaccaga
    14041 tataaaggca tccccgctac caattcgttg atagactgca gtttttagtt ggacagactc
    14101 ttcaaatagt tcttctagag cataggcttg atagacattt ttgtcttgct catcactaga
    14161 gcggagggta tggaaaatgg ccttgaaacc ataggcttct tttaaaagtc gcatgcgatt
    14221 ttccacctct tctaggctag cactgtctct tggaaacatc tctagatttt ggtcatcaat
    14281 catgagagga tcaataccga agcaatagtc agtaaaacgt gcaaacttag aaaattcata
    14341 cttggcttct aggactgaaa tcatctttgt tctcagattg agatccattg aaacgacaat
    14401 tcctcggcgc ttggcttctt ccaagagtag gagaaggatc gcacggacct cttgaccgat
    14461 agctacggtg attccactaa aatgaaaatg gctaatcccc tgaaagagag aatccatatc
    14521 tagcatgttt ggccgaatct ggctgatact cgtatgctta cgatcgtaga aaacttcact
    14581 ttgacgacaa ccaaagccgt tctccaaata gtagaggccg attcgatcgc caagccgaca
    14641 gattgaactg gtatcgattt ggtgctgttt caagaatgtg agaaaacgat ctccaatctc
    14701 gttggcaggg agtgcggtaa agactttcgt tgagataccc agagcttgca aattacaagc
    14761 gatgttaatt tctgatccgc caaaataagt cgaactggca acatgatctc cgatactggt
    14821 ggcatctaat ggtgagattc gaattaatgg ttcgccaaac agtaatattt tcgtcatcca
    14881 ttagttcctt tctggtttag aatagcttta cttggctaat acttctttca aataagcagc
    14941 gacttcatca tcttggcagt tttcaaagaa gtattcttgg aagtatttgc cagatatggt
    15001 gtctttgagg aagtcttggt cgattgtttt aaggatggtc atcaaatcat tgtgggtgat
    15061 ttgtttcacc tcgtttaaga tacgagcgtt gcgttgttca gggattacac gttctcttgg
    15121 gtagccaccg cccatttctt cagcaaataa gcgttcaaac atcaatttga gggtcagttc
    15181 tgccccccag ccaaatcctt tggcataagg gatagacaag gcgttaccac cattgatttg
    15241 agaataaagg taagcgtcag ttgggtccac tgctagacca cagacaacac cagggaagct
    15301 gtttaaagca agcatagccc ctacacccgt accacagccg gtaacaacaa agtcaactgc
    15361 ctttgtattt aaaaggatgg cagccattag tccgttctgc acataagtta attgactttc
    15421 tccttcttct ccacgcatac catagttaaa taattggtag tcttttttat ctgtcgcttc
    15481 ttttagacta tcgtaaataa tgtgattctt gctagcttga ctattttcat tgattaatgc
    15541 gattttcata agatcttctt tctattttta ctcaggttgt tttccgatgt aggctaagat
    15601 accgccatct acatatagga tgtggccatt gacaaaattg ctggcatcac tagcgagaaa
    15661 gacagcaggg cctatcaaat cttcaggatt tccccaacgt gcagcaggtg tttttgcaat
    15721 gatgaactgg tcaaatgggt ggcgagaacc atcttcttgc aactcacgaa gaggtgctgt
    15781 ttgaggagtg gcaatataac ccggtccaat tccgttacat tggatattgg ctccaccgta
    15841 ttcagacgca atgttgcggg tcaacatttt caagccccct ttagcagcag cataagcgct
    15901 aactgtttca cgtcccagtt cgctcatcat cgaacaaata ttgataatct ttccatgccc
    15961 tttctttatc atagaaggaa taactgcctt tgaaacgata aatggtgcgt ttaagtcaat
    16021 atcgatgacc ttacggaaat cagcggcgct catttcgcac attggaactc ggcggataat
    16081 accagcgtta ttaacgagga tgtcaatgac accaacctct tgttcgattt gcttgaccat
    16141 ggcttggata ccgtcctcgt ctgtcacgtc acagacatat ccatgggctt cgatgccaac
    16201 ttcacgataa gcttcaatcc ctttattgac cagatcttga ttgatatcgt taaagacaat
    16261 agtagcgccg gcttcagcgt aggatttggc aatagcaaat ccaattccat aagaagcgcc
    16321 ggtgatgaga gcaatttttc cttttaaaga aaactgctca attgagaatg atgtatttgt
    16381 catttgatac ttcctttcaa atttgttaag acttctctat aaccaaatta tattctagta
    16441 aaccggtttt gtcaataaat aaatattttt atttataaaa agatttattt tccttaaaaa
    16501 tggaatattt tgaaatttct gtctattaaa atgattaaaa agataatgaa agcgctaata
    16561 ttttaaaaaa aaactattga caagataaaa ataaaggatt acaatgggga atataaagta
    16621 aaccggtaaa cctaaacaag aaaggagaaa agatgaaaat tgtacttgta gggcatggac
    16681 attttgctac agggatttat agttctttac aattgattgc aggcaatcaa gaaaatgtgg
    16741 aggcaattga ctttgtggaa ggaatgtcag cagatgaact caagcaaaaa atcttacttg
    16801 caatttcaaa tgaagaagaa gttttaatcc taagtgatct cttgggagga tcgccattca
    16861 aggtttcttc taccataatg ggagaaaatc cagccaaaac aatgaatgtt ctctcgggtt
    16921 tgaacttagc catgttaatg gaagcagtct ttgctagaat ggctcatagc tttgatgagg
    16981 ttgttaataa atcagtagtg gcggcccagg gcggagtcgt aaatggtaaa gaattgtttt
    17041 caacggatgc agaggaagag gaagaagatt tcgaatcggg tatttaaagg gtaaaagaat
    17101 gataaaaaag gttacgattg aaaaaataaa atcgcctgag cgcttcttag aagtaccact
    17161 tctgacgaaa gaagaagtcg gccaggcaat cgataaggtt attcggcagt tagaactcaa
    17221 ccttgactat ttcaaggaag atttcccgac gccagctacc tttgataatg tctatccaat
    17281 catggataac acggaatgga ccaatggttt ctggacagga gaactgtggt tggcttatga
    17341 atacagtcaa caggatgcat ttaaaaacat cgctcataaa aatgttcttt cttttctgga
    17401 tcgtgtcaat aagagagtag aattggatca ccatgatctc ggcttcttgt acacaccgtc
    17461 ttgtatggct gaatataaga taaatggaga tggagaggct agagaagcaa ccttgaaagc
    17521 tgcagataag ttgattgaac gctatcaaga aaaaggtggt tttattcaag cttggggaga
    17581 cttgggcaag aaagagcatt accgtttgat tattgactgc ttgctcaata tccaactctt
    17641 attctttgct tatcaagaaa caggcgatca aaaatactac gatattgcag aaagccattt
    17701 ctatgcttca gctaataatg taatccgtga tgacgcttcg tccttccaca ccttctattt
    17761 tgatcctgag acaggtcaac cctttaaagg tgtaacgaga caagggtata gtgatgattc
    17821 atgctgggca cgtggtcaat catggggagt ctatggtatt cctttgactt atcgtcactt
    17881 aaaagacgag tcctgctttg acttgtttaa gggtgtgacc aattatttct tgaatcgtct
    17941 gccaaaagat catgtgtcct attgggattt gatttttaat gatggtagtg atcaatcacg
    18001 agattcttca gcaacagcta tcgccgtctg tgggattcat gaaatgctaa aacatctccc
    18061 agaggtggat gctgacaaag atatttataa acatgctatg catgccatgc ttcgttcctt
    18121 gatcgaacat tatgcaaatg atcaatttac ccctggtggg acaagtctcc tccacggtgt
    18181 gtactcatgg cattcaggta aaggagtgga tgaaggcaat atctggggtg actactatta
    18241 cctagaagcc cttatccgtt tctacaaaga ctggaaccta tattggtagg aggagaaata
    18301 tgacaatgcc aaatattatt atgacccgta tcgatgaacg gttgattcat ggacaaggac
    18361 aactttgggt aaaataccta ggttgtaata cggtcattgt tgccaatgac gaagtaagca
    18421 cggacaagat gcaacaaact ctgatgaaaa cagttgtgcc agactcagtt gccatgcgtt
    18481 tcttcccttt gcaaaaggtg attgatatca ttcacaaggc taatcctgct caaacgatct
    18541 ttatcgttgt aaaggatgtg aaggacgctt taaccttggt agaaggtggt gtccctatca
    18601 aagaaatcaa tattgggaac attcacaatg ctactggtaa agagcaagtg acacgctcca
    18661 tcttcctggg tgaagaggac aaggcagccc tcaaggaatt aagccaaact catcaagtaa
    18721 catttaatac gaaaacaact ccaacaggaa atgatggagc tgttcaagtc aacattatgg
    18781 actatattta aaagaggaga tcgttatgtc gattaatgta tttcaagcga ttttaattgg
    18841 attatggaca gctttctgtt ttagtggaat gctattagga atttacacca atagatgtat
    18901 tgttctgtca tttggtgtcg gaattattct aggtgatctg cctactgctc ttgcaatggg
    18961 agctattggt gaattggctt atatgggatt cggtgttggt gctggaggta ctgttccacc
    19021 aaacccaatc ggacctggta tctttggtac cttgatggct atcactagtg ctggtaaagt
    19081 cagtccagaa gcggctcttg ccctctctac tccgattgct gtggcgattc aattcttaca
    19141 aactttcgcc tacactgtac gtgctggtgc gcctgaaacg gcgatgaagc acttgaaaaa
    19201 ccataatttg aagaaattca agttcactct aaatgcaaca atttggttgt ttgcctttat
    19261 tggatttacc ttgggttgct tgggtgccct ttcaatggat accttgttga aactcgtaga
    19321 ctacattcca ccggtattgc ttacaggttt gacagttgct ggtaaaatgc tcccagctat
    19381 cggttttgcg atgatcttgt cagtgatggc taagaaagag ttgattccct ttgtcttgtt
    19441 gggatatgtt tgtgcagctt atctaaacat cccaacaatt ggtattgcaa ttgtaggtac
    19501 tatctttgct ttgattgaat tttataacaa gccaaaaaca gcggatcatg tggtagagga
    19561 ggaagcacac gatgactgga tctaacaaat taacaaaacg tgattatctt aaaacgtctt
    19621 tgcgggcatt cttttgtcaa aatggattta actatagtaa ctatcaaggg ttgggatatg
    19681 ccaatgtgat gtatcctgct ttgaaaaaac actatggaga gaatcaggaa ggtttctacc
    19741 aagccttgga agaaaactgt gaattctata ataccaaccc acacttcctg ccttttatta
    19801 ccagcttgca tcttgtaatg ttggaaaatg gccgtccggc aaaagaaaca cgtagcatca
    19861 agatggcctt gatgggacca ttagcaggta ttggggattc tctctctcaa ttctgtttag
    19921 ctccattgtt ctcaaccatc gcagcttcgt ttgctcaaga aggcttggtt gtcggtccaa
    19981 tcttgttctt ccttgcgatg aatacgattt taacagcgat taaattgtca actggtctgt
    20041 atggatacaa actaggaacg actgtgattg ataaactaag cgagcagatg gcaacgattt
    20101 ctcgtattgc caatattatt ggtgtaaccg taattgctgg tttggcagcg acatctgtta
    20161 agattatggt accgatcacc tttgctgcag gggaagttaa agcagacgct aaacaaagta
    20221 tcgtaagtat tcagggaatg cttgataagg ttgctccagc tcttctacca gccctattta
    20281 cacttttagt ttactacttg atcaaagaaa agaaatggac aacatataaa ctcgttattt
    20341 taacagttat catcggaatt atcggaagct ggcttaagat tatagcttaa tggatacaac
    20401 attgttttat ggaatagtga ttgtcttggc agtgagtccc cttttactgt caagctttca
    20461 ttctattcgt cagcaaaagt tgcttcgcaa acagatggag caacgacaag agtatttagc
    20521 ttctttaaca tctggtgatg aagtgttgtt attgtctgga attcatggaa aaatcatttc
    20581 tatccaagat gacttggtct ccttgcagat tgcaaaaggt gtggtcatct atgtagaaaa
    20641 ggaaagtgta atgggaaaga caaaagaact gctttttaag tagttctttt tctggtaata
    20701 gataatgaaa gaagaaagaa gacaattttt tgaaagaatc gatggaaacc aatgtcgtga
    20761 ttatatcttg tcccactgtt caaaagacta tgagaaagtc aagagttccc ttgaacgctt
    20821 gatggacaat cgttttatgt ttgatagtcc ttgggatatg gagccttgtt caaaaatcca
    20881 tcaaatccag ccgatggtat gggatcaagt atttgaagat gatccagaat gggcttatat
    20941 gctcaatcga caagaatatc tcttgcagtt tatgataggg tatgtggtag aaggagataa
    21001 ggactatatt caaaagtgca agttctttct atttgattgg attgagcagg tgagagaatt
    21061 ttctcctcaa tccttgatga ctagaacctt ggatacgggt attcgttcct ttacttggtt
    21121 gaaactactc ttgctcctct tgaaatttga cttgctagag gagaaagaac tagagaaaat
    21181 tttggtcagt ctagaaaagc agattgactt tatgaaaagc cactatcgcg ccaagtacac
    21241 ccttagtaac tgggggattt tacaaacaat tccgatgctt gctatctatc atttcttttc
    21301 agataagatg gacctagaag aagcttacca ttttgcttca gaggagttga aacagcaaat
    21361 tgagacacag attttaggag atggaagcca gtttgaacag tcgattctct atcatgtaga
    21421 ggtttataaa gccttgctgg atttgtgtct cttgcttcca gacttgcaag atagttacca
    21481 agagttgctg gaaaagatgg cgacctatat tcaaatgatg acaggcttag atggacggac
    21541 tttggctttt ggtgatagcg attctacaga aacgacagaa atgttgagcc tgtctgctgt
    21601 ggttttgaac aaggaagatc ttcttaacgg tctggatgtt aaagtcgatt tgcttagcct
    21661 cttgttcctg gggcgagaaa aggtcaagcg actgcaggaa tttgaaaaga gagcttggca
    21721 gcctaagttc atgagctttg aagactctgg acatgtctgc attaaggatg aacatcgtta
    21781 tctatttttc aaaaatggtc cgctaggaag tgcccatagc catagtgacg agaatagttt
    21841 ttgcttacag tatcaaggcc aacctatttt catagatgct gggcgttatt cttatcggga
    21901 gatatatgaa cgttatctct taaagagtgc ttggagtcat tcgacctgta ttgtagatgg
    21961 gaaagctccg gaaagaatca cgggatcctg ggaatatgaa tactatcctc actccctgtt
    22021 ttgtcaccat aaagaaaggg agggaatgca ttatattgaa ggggcttatt ggtcagcaga
    22081 acctgatttg ccttatcttc acaggagaaa aatcctcatg ctagtagagg atgtctggct
    22141 cttggtggat gacatcaggt gtcaaggtca gcatgaggcg ttgactcagt ttatccttga
    22201 caaggatgtg acctatcaag atgggaaaat caatcagttg agactatgga gtgaagttga
    22261 ttttgatttg gaagatacca tcatttctcc taaatacaat gagcttgaaa gaagtagcaa
    22321 actcaccaag cgccaattct ttgagaatca gatgctggat tataccatca ttgcgcatga
    22381 gagttttgaa atcatccgtc attctgtcta ccagacagat gatcgtgaag tggaaaatgc
    22441 tctggctttt gaagtgaaaa atgatgaaac agacaagctg attctgttat taagcgagaa
    22501 tattggtgta ggtgaaaaat tgtgcctcgt tgacggaaca aaaatgcgtg gaaaatgtct
    22561 agtatatgat aaaataaatg agagaatgat tcgcttgcag tgctagaaat aggcattttg
    22621 aatagtgaat atgttataat aagtattagt aggaggtgtt ttagattgga gaagaaactg
    22681 accataaaag acattgcgga aatggctcag acctcgaaaa caaccgtgtc attttaccta
    22741 aacgggaaat atgaaaaaat gtcccaagag acacgtgaaa agattgaaaa agttattcat
    22801 gaaacaaatt acaaaccgag cattgttgcg cgtagcttaa actccaaacg aacaaaatta
    22861 atcggtgttt tgatcggtga tattaccaac agtttctcaa accaaattgt taagggaatt
    22921 gaggatatcg ccagccagaa tggctaccag gtaatgatag gaaatagtaa ttacagccaa
    22981 gagagtgagg accggtatat tgaaagcatg cttctcttgg gagtagacgg ctttattatt
    23041 cagccgacct ctaatttccg aaaatattct cgtatcatcg atgagaaaaa gaagaaaatg
    23101 gtcttttttg atagtcagct ctatgaacac cggactagct gggttaaaac caataactat
    23161 gatgccgttt atgacatgac ccagtcctgt atcgaaaaag gttatgagca ttttctcttg
    23221 attacagcgg atacgagtcg tttgagtact cggattgagc gggcaagtgg ttttgtggat
    23281 gctttaacag atgctaatat gcgccacgcc agtctaacca ttgaagataa gcatacgaat
    23341 ttggaacaaa ttaaggaatt tttacaaaaa gaaatcgatc ccgatgaaaa aactctggta
    23401 tttatcccta actgttgggc cctacctcta gtctttaccg ttatcaaaga gttgaattat
    23461 aacttgccac aagttgggtt gattggtttt gacaatacgg agtggacttg cttttcttct
    23521 ccaagtgttt cgacgctggt tcagccctcc tttgaggaag gacaacaggc tacaaagatt
    23581 ttgattgacc agattgaagg tcgcaatcaa gaagaaaggc aacaagtctt ggattgtagt
    23641 gtgaattgga aagagtcgac tttctaaaat gaaggaaaat gacttgcaat ctctgttaag
    23701 aaataaaata atcccaccta gaacaagcta ggtgggatta tttgcctata aaatgagaaa
    23761 ttatgggagc aagctcctaa atcaactgtt tttgatctac ttctttaact acttgataaa
    23821 agttatagaa gtaggccaaa cttgaaatga tggttacgac taggaatatt gaaaatttcc
    23881 attggacagg gttggttaaa agttgtggaa aggatatgag gagaaagaag agggctgcgt
    23941 tgaggacagg tatccgtttt gattgtattt tctcaagtcc tttattgagc gcaggaagaa
    24001 agaggagtag gagtagtaaa actgtatgag aaatagctcc tgaagtaagg gcgaagaaaa
    24061 ggaaaatact gataaaaaca tgaatgatca gtagtctagc tagtgatttc ataaggcacc
    24121 tcctaatcct ggtctttttt agctcttgca atacgaagtg agtcgacaat atgtatcatc
    24181 actccgaaaa agaaagctcc cagtatagtt ttaaaaatat gttttgtatt tagaagagaa
    24241 ctgataaaat ttggattttc acttgttagg gtatcaatga gtggaattat aaaaaatatc
    24301 actgttccat aaatcgaacc tgctttcaga ccaggataac gtaactgttt cttttctttt
    24361 ttcatgagtt tcctcctaat cctcatcttg atttttctta gtttttgcaa tgcgacggga
    24421 gatgaggaac tgtatgctcg ctccgaagaa aatagaaccg agaatacttg atacaccatt
    24481 tcttatagtg agaagagaat gaaaatagtc ctgaccttca tctatgagta tactgagaag
    24541 aggagttata aaaaacatcc atagaccaaa gaacaaacct gctttcagac cagggtagtg
    24601 tagttgcttg ctttctttct cattcagcat atctggttca atgactgtga tgcctgtttt
    24661 tttcatttgg taggtgacat agccagaagc gatgagggca atcactaaaa tcagaggagg
    24721 atagattaga gccacttctt gagggtattt ataggccaga aggagtggaa taatatttcc
    24781 gaaaatcatc agataaaaga ggacgataaa gacttggttc ccaatactat cggcctcacg
    24841 ccgtttgtat tcgtcaaggg gaccagaaat accgtatgtg cgtttgatca gtttttcagt
    24901 gaaggtttct tttttcatga gtttgcttct tttttaaaaa tcatcctccc aaaagagact
    24961 gttgaggtca gtttggaggc tgcgggcgag attgagacag agttccaagg ttggattgta
    25021 cttgtcgttt tcaatcatat tgatagtctg tctcgagaca ccgatatcct tggcgagttc
    25081 gagctgggaa atacccaatt ccttgcgaaa ttctttcaca cgattcatct ggtctccttt
    25141 ctgatttatg tcgtatatat ttgactatat tatagtcttt taaacataaa gtgtcaagta
    25201 tttttgacat attttttgaa gaaatagtag tctccttgtc ctatttgtct gacaagtgca
    25261 agctggtcgg atttgtggta aaatagataa gatatgacaa aagaatttca tcatgtaacg
    25321 gtcttactcc acgaaacgat tgatatgctt gacgtaaagc ctgacggtat ctacgttgat
    25381 gcgactttgg gcggagcagg ccatagcgag tatttattaa gtaaattaag tgaaaaaggc
    25441 catctctatg cctttgacca ggatcagaat gccattgaca atgcgcaaaa acgcttggca
    25501 ccttacattg agaagggaat ggtgaccttt atcaaggata acttccgtca tttacaggca
    25561 cgtttgcgtg aagctggtgt tcaggaaatt gatggaattt gttatgactt gggagtgtct
    25621 agtcctcaat tggaccagcg tgagcgtggt ttttcttata aaaaggatgc gccactggac
    25681 atgcggatga atcaggatgc tagtctgaca gcctatgaag tggttaatca ttatgactat
    25741 catgatttgg ttcgtatttt cttcaaatac ggtgaggata aattctctaa acagattgcg
    25801 cgtaagattg agcaagcgcg tgaagtgaag ccgattgaga caacgactga gttagcagag
    25861 attatcaagt tggtcaaacc tgccaaggaa ctcaagaaga aggggcatcc tgctaagcag
    25921 attttccagg ctattcgaat tgaagtcaat gatgaactgg gagcggcaga tgagtccatc
    25981 cagcaggcta tggatatgtt ggctctggat ggtagaattt cagtgattac ctttcattcc
    26041 ttagaagacc gcttgaccaa gcaattgttc aaggaagctt caacagttga agttccaaaa
    26101 ggcttgcctt tcatcccaga tgatctcaag cccaagatgg aattggtgtc ccgtaagcca
    26161 atcttgccaa gtgcggaaga gttagaagcc aataaccgct cgcactcagc caagttgcgc
    26221 gtggccagaa aaattcacaa gtaagaggga aaaagatggc agaaaaaatg gaaaaaacag
    26281 gtcaaatact acagatgcaa cttaaacggt tttcgcgtgt ggaaaaagct ttttactttt
    26341 ccattgctgt aaccactctt attgtagcca ttagtattat ttttatgcag accaagctct
    26401 tgcaagtgca gaatgatttg acaaaaatca atgcgcagat agaggaaaag aaaaccgaat
    26461 tggacgatgc caagcaagag gtcaatgaac tattacgtgc agaacgtttg aaagaaattg
    26521 ccaattcaca cgatttgcaa ttaaacaatg aaaatattag aatagcggag taagatatga
    26581 agtggacaaa aagagtaatc cgttatgtga ccaaaaatcg gaaatcgccg gctgaaaaca
    26641 gacgcagagt tggaaaaagt ctgagtttat tatctgtctt tgtttttgcc atttttttag
    26701 tcaattttgc ggtcattatt gggacaggca ctcgctttgg aacagattta gcgaaggaag
    26761 ctaagaaggt tcatcaaacc acccgtacag ttcctgccaa acgtgggact atttatgacc
    26821 gaaatggagt cccgattgct gaggatgcaa cctcctataa tgtctatgcg gtcattgatg
    26881 agaactataa gtcagcaacg ggtaagattc tttacgtaga aaaaacacaa tttaacaagg
    26941 ttgcagaggt ctttcataag tatctggaca tggaagaatc ctatgtaaga gagcaactct
    27001 cgcaacctaa tctcaagcaa gtttcctttg gagcaaaggg aaatgggatt acctatgcca
    27061 atatgatgtc tatcaaaaaa gaattggaag ctgcagaggt caaggggatt gattttacaa
    27121 ccagtcccaa tcgtagttac ccaaatggac aatttgcttc tagttttatc ggtctagctc
    27181 agctccatga aaatgaagat ggaagcaaga gcttgctggg aacctctgga atggagagtt
    27241 ccttgaacag tattcttgca gggacagacg gcattattac ctatgaaaag gatcgtctgg
    27301 gtaatattgt acccggaaca gaacaagttt cccaacgaac gatggacggt aaggatgttt
    27361 atacaaccat ttccagcccc ctccagtcct ttatggaaac ccagatggat gcttttcaag
    27421 agaaggtaaa aggaaagtac atgacagcga ctttggtcag tgctaaaaca ggggaaattc
    27481 tggcaacaac gcaacgaccg acctttgatg cagatacaaa agaaggcatt acagaggact
    27541 ttgtttggcg tgatatcctt taccaaagta actatgagcc aggttccact atgaaagtga
    27601 tgatgttggc tgctgctatt gataataata cctttccagg aggagaagtc tttaatagta
    27661 gtgagttaaa aattgcagat gccacgattc gagattggga cgttaatgaa ggattgactg
    27721 gtggcagaat gatgactttt tctcaaggtt ttgcacactc aagtaacgtt gggatgaccc
    27781 tccttgagca aaagatggga gatgctacct ggcttgatta tcttaatcgt tttaaatttg
    27841 gagttccgac ccgtttcggt ttgacggatg agtatgctgg tcagcttcct gcggataata
    27901 ttgtcaacat tgcgcaaagc tcatttggac aagggatttc agtgacccag acacaaatga
    27961 ttcgtgcctt tacagctatt gctaatgacg gtgtcatgct ggagcctaaa tttattagtg
    28021 ccatttatga tccaaatgat caaactgctc ggaaatctca aaaagaaatt gtgggaaatc
    28081 ctgtttctaa agatgcagct agtctaactc ggactaacat ggttttggta gggacggatc
    28141 cggtttatgg aaccatgtat aaccacagca caggcaagcc aactgtaact gttcctgggc
    28201 aaaatgtagc cctcaagtct ggtacggctc agattgctga cgagaaaaat ggtggttatc
    28261 tagtcggggt aaccgactat attttctcgg ctgtatcgat gagtccggct gaaaatcctg
    28321 attttatctt gtatgtgacg gtccaacaac ctgaacatta ttcaggtatt cagttgggag
    28381 aatttgccaa tcctatcttg gagcgggctt cagctatgaa agactctctc aatcttcaaa
    28441 caacagctaa agctttggag caagtaagtc aacaaagtcc ttatcctatg cctagtgtca
    28501 aggatatttc acctggtgat ttagcagaag aattgcgtcg aaatcttgta caacccatcg
    28561 ttgtgggaac aggaacgaag attaaaaaca gttctgctga agaagggaag aatcttgccc
    28621 cgaatcagca agtccttatc ttatctgata aagcagagga ggttccagat atgtatggtt
    28681 ggacaaagga gactgctgag acccttgcta agtggctcaa tatagaactt gaatttcaag
    28741 gctcgggctc tactgtgcag aagcaagatg ttcgtgctaa cacagctatc aaggacatta
    28801 aaaaaattac attaacttta ggagactaat atgtttattt ccatcagtgc tggaattgtg
    28861 acatttttac taactttagt agaaattccg gcctttatcc aattttatag aaaggcgcaa
    28921 attacaggcc agcagatgca tgaggatgtc aaacagcatc aggcaaaagc tgggactcct
    28981 acaatgggag gtttggtttt cttgattact tctgttttgg ttgctttctt tttcgcccta
    29041 tttagtagcc aattcagcaa taatgtggga atgattttgt tcatcttggt cttgtatggc
    29101 ttggtcggat ttttagatga ctttctcaag gtctttcgta aaatcaatga ggggcttaat
    29161 cctaagcaaa aattagctct tcagcttcta ggtggagtta tcttctatct tttctatgag
    29221 cgcggtggcg atatcctgtc tgtctttggt tatccagttc atttgggatt tttctatatt
    29281 ttcttcgctc ttttctggct agtcggtttt tcaaacgcag taaacttgac agacggtgtt
    29341 gacggtttag ctagtatttc cgttgtgatt agtttgtctg cctatggagt tattgcctat
    29401 gtgcaaggtc agatggatat tcttctagtg attcttgcca tgattggtgg tttgctcggt
    29461 ttcttcatat ttaaccataa gcctgccaag gtctttatgg gtgatgtggg aagtttggcc
    29521 ctaggtggga tgctggcagc tatctctatg gctctccacc aagaatggac tctcttgatt
    29581 atcggaattg tgtatgtttt tgaaacaact tctgttatga tgcaagtcag ttatttcaaa
    29641 ctgacaggtg gtaaacgtat tttccgtatg acgcctgtac atcaccattt tgagcttggg
    29701 ggattgtctg gtaaaggaaa tccttggagc gagtggaagg ttgacttctt cttttgggga
    29761 gtgggacttc tagcaagtct cctgaccctc gcaattttat atttgatgta agaatggcac
    29821 cctgatgttt cagggtgttt ttgtgtttaa atacacaatg aaaatcaaag aacaaactag
    29881 aaagctaact ttaggctgct caaaacatag tagtttgaaa ctagaatagt acacatctac
    29941 ttctaaaata ttgttagaaa tcgatttgac tgtcctgatc gacttgtcct gttcttattt
    30001 cattttacta tacagttttg agatggttga tagaactgac gaagtcagct caaaacacgg
    30061 ttttgaggtt gtagataagg agaagctgat gtggtttgaa gaggttttcg aagagtgtaa
    30121 aaaaatattg gtcagttaaa gtcaaaacta ttgacctttt ctgaccaatg tagtatatta
    30181 tgaacgtaag gtaaatgaaa gccttactag aacacttatt taaagtaaaa tagaaagagg
    30241 tgggtctatg tttcatctag aaatcttcag aagtaaagat ggtctactcc tgcttgaaaa
    30301 agaaaaacca gaaatagtac gtagggtagc ggtttagcta gtctaaattt tttaaaacaa
    30361 aggtcaaaga tagtcaatat cagtaatcat aactaagtaa acaaaaagag gtaaagaata
    30421 tgaataacaa ctttaataat tttaataaca tggatgattt atttaaccaa ttgatgggtg
    30481 gtatgcgagg atatagttct gaaaatcgcc gttacttaat taatggacgc gaagtcacac
    30541 ctgaggaatt tgctcactat cgtgcgactg gtcaattacc aggaaatgca gaaactgatg
    30601 tgcaaatgcc acaacaggca tcaggtatga aacaagacgg tgtccttgca aaactaggtc
    30661 gaaacttaac agcagaagcg cgtgagggca agttggatcc tgttatcgga cgaaacaagg
    30721 aaattcaaga aacatctgaa atcctctcac gccgcaccaa gaacaatcct gttttggtcg
    30781 gagatgcagg tgttggtaag acagcagttg tcgaaggtct agcgcaagcc attgtgaacg
    30841 gagatgttcc tgctgctatc aagaacaagg aaattatttc tattgatatc tcaggtcttg
    30901 aggctggtac tcaataccgt ggtagctttg aagaaaatgt ccaaaactta gtcaatgaag
    30961 tgaaagaagc agggaatatt atcctcttct ttgatgaaat tcaccaaatt cttggtgctg
    31021 gtagcactgg tggagacagt ggttctaaag ggcttgcgga tattctcaag ccagctctct
    31081 ctcgtggaga attgacagtg attggggcaa caactcaaga cgaataccgt aacaccatct
    31141 tgaagaatgc tgctcttgct cgtcgtttca acgaagtgaa ggtcaatgct ccttcagcag
    31201 agaatacttt taaaattctt caaggcattc gtgacctcta tcaacaacac cacaatgtca
    31261 tcttgccaga cgaagtcttg aaagcagcgg tggattattc tgttcaatac attcctcaac
    31321 gtagattgcc agataaggct attgaccttg tcgatgtaac ggctgctcac ttggcggctc
    31381 aacatccagt aacagatgtg catgctgttg aacgagaaat cgaaacggaa aaagacaagc
    31441 aagaaaaagc agttgaagca gaagattttg aagcagctct aaactataaa acacgcattg
    31501 cagaattgga aaggaaaatc gaaaaccaca cagaagatat gaaagtgact gcaagtgtca
    31561 atgatgtggc tgaatccgtg gaacgaatga taggtatccc agtatcgcaa atgggagctt
    31621 cagatatcga acgtttgaaa gatatggctc atcgcttgca agacaaggtg attggtcaag
    31681 ataaggccgt agaagctgta gctcgtgcta tccgtcgtaa ccgtgctggt tttgatgaag
    31741 gaaatcgccc aatcggcagc ttcctctttg tagggtctac tggggttggt aagacggagc
    31801 ttgctaagca attggcactc gatatgtttg gaacccagga tgcgattatc cgtttggata
    31861 tgtctgaata cagtgaccgc acagctgttt ctaagctaat tggtacaaca gcaggctatg
    31921 tgggttatga tgacaatagc aataccttaa cagaacgtgt tcgtcgcaat ccatactcta
    31981 tcattctctt ggatgaaatt gaaaaggctg accctcaagt tattaccctt ctcctccaag
    32041 ttctagatga tggtcgtttg acagatggtc aaggaaatac agtaaacttc aagaacactg
    32101 tcattattgc gacctcaaat gctggatttg gctatgaagc caacttgaca gaagatgcgg
    32161 ataaaccaga attgatggac cgtttgaaac ccttcttccg tccagaattc ctcaaccgct
    32221 ttaatgcagt catcgaattc tcacacttga ctaaggaaga cctttctaag attgtagatt
    32281 tgatgttggc tgaagttaac caaaccttgg ctaagaaaga cattgacttg gtagtcagtc
    32341 aagcggctaa agattatatc acagaagaag gttacgacga agtcatggga gttcgtcctc
    32401 tccgtcgagt ggttgaacaa gaaattcgtg ataaggtgac agacttccac ttggatcatt
    32461 tagatgctaa acatctggaa gcagatatgg aagatggcgt tttggttatt cgtgagaaag
    32521 cctaagatag aattttgagg ataaaaaaga aggagccagc tgaaaaaaaa ctggttcctt
    32581 ttgaggtacg acgggcatgt cgtatagtag aagtgtatta ttctagtttc aatttactat
    32641 agtagctcag aagtcggtac ttaaacgtgc tatatcaaaa ccagtccttg aaaaacgtgg
    32701 actggtttcg tgtttggatt attaccttga acgacatgcg ttaaaagtta gttgaaccgc
    32761 cgtatgccga acggcacgta cggtggtgtg agaggggcta gagattatcc cctactcgat
    32821 tttaaatcac atgacgttca aaggcatcat ctgaaatccc ttgttccaag atgagttttg
    32881 cccattcttt agcagagaag aggctgtggt ccttgtagtt tccgcaagat tcgatggttg
    32941 tccctgggac atcttcccaa gtagtagttt cagcgatttc cttgagcgaa tccttgataa
    33001 cagctgcgat tttagcactg gtgtgacgtc cccacataat catgtggaag cctgtgcggc
    33061 aaccaaatgg tgaacagtca atcatgccgt caatgcgggt acggatgagt ttggctaaga
    33121 ggtgctcgat agtgtgaagg ccggcggtag ggatagagtc ttcgtttggt tgcaccaagc
    33181 gaatatcata attggagatg atgtctcctt ttggtcctgt ttcttcccca atcaagcgaa
    33241 catagggtgc tttgacaatg gtgtggtcaa gttcaaaact ttcgacaata acttcttttg
    33301 acatggtaaa tcctttcagt tttcttctct cattatatca taaaggttgc tcctgagaca
    33361 gagagaaaac ctctccgagg ctggagaggt tgaaatcttt acttacgata taagcggtcg
    33421 tattggtagt atgggtcaaa ggttacgttg atatccagtt tacgaaggac attcttgtct
    33481 tcatcagtca agatgatggt tgagtgggct tcgcttcctt tgaggttgcc gagttcttcc
    33541 atagcgcggg cagcatcagg attttctgta gctgtgatag caagtgcaat caggatttca
    33601 tttgaatgaa ggcgtggatt gcggctaccg agatgatcga ttttaagacc ttggattggc
    33661 ttaacaactt caggttcgat tagttttact tctttagcga tgtcagctga ttttttgatg
    33721 gcgttgatca aggcagcggc tgtaggacca aagagttctg agttcttacc agtgacgatt
    33781 tccccatttg gcaattcaaa ggctagggct ggtccaccag tttcttctgc tttttggcgc
    33841 gcaacgacag caaccttacg gtctgcaggt gtgataccga ggtcgttcat gagcaactca
    33901 attttcttga cggcagcttc gccaactttt tcagctttga agtcaagaat tgtttgatag
    33961 taacggcgga tgatttcttg tttagaagct tcgacagcgg cctcgtcatc tgtaatagcg
    34021 aaaccaacca tgttgacacc catatctgtc ggtgaagcgt atggtgattt tccgagaata
    34081 cgttccaaca tgcgtttgag cactgggaag atttcgatat cacggttgta gttgacagtg
    34141 gtttctccat aggtttgaag atggaagggg tcaatcatgt tgacatcatc aaggtcagct
    34201 gtggcagctt cataagccaa gttaactgga tgatgaaggg gaagattcca aacagggaag
    34261 gtttcaaatt tagcgtagcc agacttgatg ccattgattt ggtcgtggta catattggac
    34321 atacacgttg ccaattttcc agaaccgggt ccaggagcgg ttacgacaat caagttgcga
    34381 ctggttttga tgtagtcgtt tttgcccatg ccttctgggg aaatgatgtg atccatatcc
    34441 gtcggatatc ctttgattgg ataatgaaga taagaatcaa ttctgttttt ctcaagttgg
    34501 ttgcggaagg catctgcagc gagttggcca gcgtattgtg taatgacaac ggaaccaaca
    34561 aaaatcccta attcattgaa tttgtcaatc aaacgaagaa cttcttggtc ataagaaatg
    34621 cctaagtcgc cacgtgcttt ggaatgttca atgttgctag cattaatggc aatcacaacc
    34681 tcaacttgct ctttcaattc ttgcaagagc ttgattttgt tgtcaggttc ataaccagga
    34741 aggacacgag cagcgtggaa atcttctaac attttgccac caaactctag gtaaagcttg
    34801 ccgtcaaatt ggttaatgcg atctaagatg tggtcgcgtt gtagatttaa atattgttca
    34861 gaactaaaag cttgtttttt cattttttta cctctggact ctattataat aaaaaattgg
    34921 aagttaggaa actacggagc taaaaaagaa attaaaaaga ttaagcaaac gcttgcacaa
    34981 aattttaaaa agtgctatta tagactatag attatgaaaa taatgaggta agaagatgca
    35041 agaaaaatgg tggcataatg ccgtagtcta tcaagtctat ccaaagagtt ttatggatag
    35101 taatgaagat ggaattggcg atttgccagg aattaccagt aagttggact atctagccaa
    35161 gctaggaatc acagcaattt ggctttctcc cgtttatgac agccctatgg atgataatgg
    35221 ctacgatatt gctgattatc aagcgattgc ggctattttt ggaaccatgg aggacatgga
    35281 tcagctgatt gcagaagcta agaagcgtga tatccgtatc atcatggact tggtggtcaa
    35341 tcatacctcg gatgagcatg cttggtttgt cgaagcctgt gaaaatactg acagccctga
    35401 gcgagactac tatatctggc gcgatgaacc caatgaccta gattctatct ttagtgggtc
    35461 tgcttgggaa tacgatgaaa agtcaggtca atactatctc cactttttca gcaagaaaca
    35521 gccggatctc aactgggaaa atgaaaaact tcgccagaaa atttatgaga tgatgaactt
    35581 ctggattgat aaaggtattg gtggtttccg tatggatgtt attgacatga ttggcaaaat
    35641 tcctgacgag aaggtagtca ataatggtcc tatgctccat ccctatctca aggaaatgaa
    35701 tcaggcgacc tttggagata aggagctctt gacagtaggg gagacttggg gagcaacgcc
    35761 agagattgcc aagctctact ctgatccaaa ggggcaagaa ttgtctatgg tcttccagtt
    35821 tgaacatatc ggtcttcagt atcaggaagg tcagcctaaa tggcactatc aaaaagagct
    35881 gaatattgct aagttaaaag aaatcttcaa caaatggcag acagagttag gagttgagga
    35941 cggctggaat tccctcttct ggaacaacca tgacctccct cgtattgtct caatctgggg
    36001 aaatgaccaa gaataccgtg aaaaatctgc caaagccttt gcgattttgc ttcatctcat
    36061 gagagggact ccttatatct accaaggtga ggagattggg atgaccaact atccgtttga
    36121 aacactggat caagtagaag atattgaatc tctcaactat gcgcgtgagg ctcttgaaaa
    36181 aggtgttccg attgaagaaa tcatggacag tatccgtgtt attggacgtg acaatgcccg
    36241 tacccctatg caatgggacg agagcaaaaa cgctggtttc tcaacaggtc aaccttggtt
    36301 ggcggttaat ccaaactacg aagaaatcaa cgttcaagaa gcactggcaa actcagattc
    36361 tattttctat acctatcaga aactcatcca gattcgtaag gaaaacagct ggttgattcg
    36421 agctgatttt aaattactcg atacggctga taaggtcttt gcttatatcc gtaaggacgg
    36481 tgaccgtcgc ttcctagtcg tggctaattt atccaatgat aaacaaaact tttcagtaga
    36541 tggaaaagtt agatctgtct tgattgaaaa cactgcggct aaagaagtac ttgaaaaaca
    36601 ggtcttgact ccatgggatg ctttctgtgt ggaaatgact gactagaatg agcaagagcc
    36661 tgggacaaaa tagttctcga ccacaaaaaa gctagagatt tccaattgtg gaactctagc
    36721 tttttaattt tgagtcattc tcttattgta ttttaggagg ttattggcca taagtaccaa
    36781 tcccatgtca attctcactt gacgcttgcc cctcagatta catctcttgt aacccaaaca
    36841 agcctttatc tgcccaaaga caggttccac atcaatcttg cgttgagcga aaatctgtct
    36901 accttgggga gataaaagcg cctgacattc tttagctttc aagttttgat agccttcgtt
    36961 catatacagt ccccttttga ggggctgatt caggttcgtc gacgtagtaa accttgattt
    37021 cttgttgaaa gtctgtctgt gttttctgat gtttggtatg gtgaaaacga taatactagc
    37081 catcaggatg tgtatagcta tcctccttgt cattatagtg ccaattcgct aagtttttag
    37141 ctgactgttt atagcctctc ttctgttcct tatcaaacat ggcatattta atcagatggt
    37201 ttacctcctt ttcatctaaa cgaaggaggt tctcttcact tccatatcca gcatctgcga
    37261 caactgtctt caagtcatgc ggataggttt caaggaatgg cagaagagtc ttggtatctg
    37321 tcggatttga aaagacatca tagtgaagaa caaattggtt ttccgtagcg atttgaagat
    37381 tataagcagc cttgggttga ctatttttca tatgttcttc cttcatccgc ataaaagtgg
    37441 catctggatc ggttttggaa aaactgttag gcccttgaaa tgtctcctga tagttctcat
    37501 atttttcagc acgtactgaa aaatcctcct ttaaactctt cataatcctt tttattttta
    37561 tgaagatatt gtttgaaaga tgtgagtttc cacggatggg tttgtggagg gatatacttg
    37621 cgtctttctt tttttgttct ggttcttgtt caaggttttt cgaatagagt tcatgattta
    37681 gtagctcctt tgtgtgatag attttgtcag cgatattgag gtagatgtca ccatcaaatg
    37741 cttttataac taatgctttt gtctttctga tgaaatagac ttcttttcct tgctcggtag
    37801 ggatatagca acgattttgg aatcggatat ggtgtccact atcgacgact ctctcccgcc
    37861 agtttagcta gaatgagatt tcgttcagag ggcttaggag cctcctcaaa gacagagagt
    37921 tttgtcttgt ttccaaactg ttcattaaag gtttggatat aggaaggcag aaaggtattg
    37981 gcttcttcca aggtatgaat cttgtttcgt tccagttcga taggcaagag agattgtagt
    38041 atctgattga gtctttcgac tctcccttta gcttgaggga tagaggttgt ctcaaggaga
    38101 attcccagtt ggtgacaggc gtatccaaat tgtgtatggg tgtcgtcctc cattttctta
    38161 gagttggagg cttgataggt aaaaacagtt ctcttatctg ttttgatttg aacaggaatg
    38221 ccgtggttgg ctaagatttg ttcgaggaca tgatagtaag cattcaaggt ctcttgtttg
    38281 tcaaaataag cgcctaggat attgccagaa gcatcatcaa tggctaggtg taagttagag
    38341 gtttctgctc caaaccaggc atgagggctg gcatccattt ggatgagttc tccagcaaat
    38401 ttctttctgg gtctactagg atgtaccttt ttagggtctt ccaggaagtt ttcagccgtc
    38461 ggtaagattg gattgtctag gggttgcttg gggttcagtt tagcttgctt tcttactctc
    38521 ttctttgtct ttctatggga cttaggcgac aggatatttt tcttatagag tatttttcta
    38581 acagtagtat cagagagctg aattccttct tcttcagcta gcaattcaca gaaatgacgg
    38641 acatttggtt tatatgtttc ataggagagg tatttcttta ggatacgttc tttgatttca
    38701 tcagggattg catgttttgg ttttcgattt ctgtttccgt gtctgaaggc ttcttttcct
    38761 ttctgttgat aggctagtag cagacgattg atttgccttt cagaaagatt gagctcgaca
    38821 caggctcgtt tctttgtttt ctttccttgg gctatagctt ttatcacaag atattttttc
    38881 gtttcattca tattcagttg gatcctttac atatgactat tctaccaaat gggacatttt
    38941 tacgttcgat ttactaaaga cattatcaca ttcgaattac acaagatgca gatagtgaaa
    39001 aaaggtgtag acattaccgt aaaaaagtga tataattgta agatgttcaa tgtataggtg
    39061 ttaatcatga gtagacgttt taaaaaatca ggttcacaga aagtgaagcg aagtgttaat
    39121 atagttttgt tgactattta tttattgtta gtttgttttt tattgttctt aatctttaag
    39181 tacaatatcc ttgcttttag atattttaat ctagtggtaa ctgcgttagt cctactagtt
    39241 gccttggtag ggctactctt gattatctat aaaaaagctg aaaagtttac tatttttctg
    39301 ttggtgttct ctatccttgt cagctctgtg tcgctctttg cagtacagca gtttgttgga
    39361 ctgaccaatc gtttaaatgc gacttctaat tactcagaat attcaatcag tgtcgctgtt
    39421 ttagcagata gtgatatcga aaatgttacg caactgacga gtgtgacagc accgactggg
    39481 actgataatg aaaatattca aaaactacta gctgatatca aatcaagtca gaatatcgat
    39541 ttgacggtca atcaaagttc gtcttacttg gcagcttaca agagtttgat tgcaggagag
    39601 actaaggcca ttgtcttaaa tagtgtcttt gaaaatatca tcgaatcgga gtatccagat
    39661 tacgcatcga agataaaaaa gatttatacc aagggattca ctaaaaaagt agaagctcct
    39721 aagacgtcta agaatcagtc tttcaatatc tatgttagtg gaattgatac ttatggtccg
    39781 attagttcgg tgtcgcgttc agatgtcaat atcctgatga ctgtcaatcg agataccaag
    39841 aaaattctct tgaccacaac gccacgtgat gcttatgtac caatcgcaga tggtgggaat
    39901 aatcaaaaag ataaattgac ccatgcgggc atctatggag ttgattcgtc cattcacacc
    39961 ttagaaaatc tctatggagt ggatatccat tactatgtcc gtttgaactt cacttctttc
    40021 ttgaaattga tcgatttgtt gggtggggta gatgtttata atgaccaaga tttcacatct
    40081 ctacacggga agttccattt cccagttgga aatgtccact tagattctga gcaggctctg
    40141 ggatttgttc gtgagcgcta ttccctagcg gatggtgatc atgaccgcgg gcgcaatcaa
    40201 caaaaggtga ttgcggctat ccttcaaaaa ttaacttcga gcgaagcgct gaaaaattat
    40261 agtatgatca ttgatagctt gcaagattct atccaaacaa acatgcccct tgagaccatg
    40321 ataaacttgg tcaatgctca gttagaaagt ggtggaacgt acaaagtaaa ttcgcaagac
    40381 ttgaagggta ggggacggac ggatcttcct tcctatgcga tgccagatag taacctctat
    40441 atgatggaaa ttaacgacag tagccttgca tctgtcaaaa ctgctattca ggatgtgttg
    40501 gagggcagat gaaatgattg atattcattc gcacattgtc tttgatgtag atgatggtcc
    40561 caagtcaaga gaggaaagta aggctctctt gacagaagcc tacaggcagg gggtgcgaac
    40621 cattgtctct acctctcacc gtcgcaaggg catgtttgaa actccagaag agaagatagc
    40681 agaaaacttt cttcaggttc gggaaatagc taaggaagtc gcgagtgact tggtcattgc
    40741 ttatggggcc gaaatttact acacaccaga tgttttgggt aagctggaaa aaaatcggat
    40801 tccgaccctc aataatagtc gttatgcctt gatagagttt agtatgaaca ccccttatcg
    40861 cgatattcat agtgccttga ttaaaatatt gatgttggga attactccag tcattgccca
    40921 catcgagcgc tatgatgctc ttgaaaataa tgaaaaacgc gttcgagagc tgattaatat
    40981 gggctgttac acgcaagtaa atagttcaca tgtcctcaaa tccaaacttt ttggagaacc
    41041 ttataaattc atgaaaaaaa gagctcagta tttcttggag cgtgatttgg ttcatgtgat
    41101 tgcaagtgat atgcataatg tggacagcag acctccccat atggcagaag catatgacct
    41161 tgtttcccaa aaatacggag aaacgaaggc tcaggacctt tttatagaca atcctcgaaa
    41221 aattgtaatg gatcaactaa tttaggagaa ataatgaaag aacaaaacac gatagaaatc
    41281 gatgtatttc aattacttaa aaccttgtgg aaacacaagc taatcatttt attagtggca
    41341 cttgtgacag gggcgggagc ttttgcatat agcattttta ttgttaagcc agaatataca
    41401 agcaccacgc gtatttacgt agtcaatcgt aatcaagaaa ataagccggg actgacgaat
    41461 caggacttgc aggcaggaac ttatctggta aaagactacc acgaaattat cctttcgcaa
    41521 gatgtattgg aaaaggtagc gacaaatttg aaattggata ttccagtaaa aacgttaact
    41581 agcaaagtgc aagtgactgt accagccgac actcgtatcg tctcaatctc tgtcaaggat
    41641 aaacaaccag aggaagccag tcgcattgct aattctatac gagaagttgc tgcagaaaag
    41701 atcatcgctg taacgcgagt atctgatgta acgacgcttg aagaagcgcg accagctacg
    41761 actccctctt ctccaaatgt tcgacgcaac accttagttg gttttcttgg agccgccgcc
    41821 gtaacagtaa ttactgttct tttgattgag ttgttcgata cccgtgtaaa acgtcctgaa
    41881 gaagttgaag atgtactgca aatgccactt ctaggagtcg ttccagattt taataaaatg
    41941 aaataggagg aagttatgcc aacgttagaa atctcacagg cgaaattaga acttgcgaaa
    42001 aagacagagg aatattataa tgctttgtgc acgaacctac agttaagtgg agatgatttg
    42061 aaagtatttt ctatcagttc tgtgaaagca ggagaaggaa aaacaacgac ttccaccaat
    42121 atcgcttggg cttttgcgca tgcaggttac aaaacgctgc tgattgatgc agacatgcgt
    42181 aactccgtta tgtcaggtgt ctttaaatca agggaaagga ttactgggct aacagaattt
    42241 ctatcaggga caacagacct gtcacagggg ctttgcgata ccaatgttga gaatctcttt
    42301 gtgattcagg ctggctctgt gtcaccaaat ccgatagccc ttcttcaaag taagaatttc
    42361 agtacaatgc ttggaacctt gcgtaaatat tttgactata tcgtcgtaga cactgctcct
    42421 atcggtatcg tgattgatgc ggctatcatt atgcagaaat gtgatgcttc tattttagtg
    42481 acgaaggcag gtgaaacaaa acgacgggaa cttcaaaaag cgaaagaaca gttggaacaa
    42541 actgggaagt cgtgtttagg agttgtgttg aataaattcg atacttcagt agacaaatac
    42601 ggtttttatg ggagttatgg gagttatggg agttacagga aacagaaaaa ataggttgag
    42661 ggatagagat gaatgaaaaa ctagcaaagt cttcagtagc tatagtccag agttttcttg
    42721 ttattttatt gacttatcta cttagcgctg tgagagaaac agagattgtt tcaacaacag
    42781 ctattgtact ttatatcctc cactattttg tcttttatat cagtgattat ggacggaatt
    42841 tctttaaaag gagatatttg attgaacttg tccagacatt gaaatatatc ctattctttg
    42901 cgctagcgat tagtatttct aattttttct tagaggatcg atttagtatt tccagacgag
    42961 gcatgattta cttcctccta ttacatgttt tcttagtcta tatgctaaac ctatttatca
    43021 agtggtattg gaagcgggct tatcccaact ttaaaggaag taagaaggtt ttcctactta
    43081 cagcaacttc tcatgtcgaa aaggtactgg atagactaat agaatcagat gatgttgttg
    43141 gggagttggt agccgtcagt gttttagata aaccagattt tcagcatgat gatttaaagg
    43201 ttgtagcaga gggggagatc gtaaactttg cgactcgtga ggtggtcgat gaagtcttta
    43261 tcaatcttcc aagtgaaaaa tacaatattg gagagcttgt ctctcagttt gaaacgatgg
    43321 gaattgatgt aacagtcaat ctaaatgctt tcgattgggc acgtaacaag caaatttgtg
    43381 agatggcagg actaaatgtt gtgacttttt ctacaacatt ttataagact agccatgtga
    43441 ttgctaagcg ggttattgat attatcggtt ccctggtagg tttgatacta tgtggtctag
    43501 tcagtattgt gctggttcct ttgattcgaa aggatggggg ctctgctatt tttgctcaga
    43561 cgcgtatagg aaaaaatggc cgccatttca ctttttacaa gtttcgctct atgtgtgtag
    43621 atgctgagga taaaaagaga gaactcatgg aacaaaatac catgcagggt ggaatgttta
    43681 aggtggacga tgatcctcgt atcacgaaaa ttggtcattt tatacggaag acgagcttgg
    43741 acgagctacc acagttttat aatgttctaa agggagatat gagtttggta ggtacacgac
    43801 caccaacagt ggacgagtat gaacactata ccccagaaca aaaacgtcgg ctaagtttta
    43861 aacctggtat aacaggctta tggcaggtca gtggacgaag tgaaatcaaa aatttcgatg
    43921 aagttgtcaa attagatgtg gtctatattg atggttggac aatctggaaa gatattgaaa
    43981 ttttattgaa gacagttaaa gttgtattga tgaaggatgg agcgaaatag tttgcttttg
    44041 tttcagacta ttaggagaaa aatgaagaag tcagtttata tcattggttc aaaagggatt
    44101 cccgctaagt atgggggctt tgaaaccttt gttgagaaat taacagaata tcaaaaagat
    44161 ggtaacatcc aatactatgt tgcctgcatg cgcgaaaatt ctgcaaaatc aggatttaca
    44221 gcagatacat ttgagtacaa tgatgctatt tgttacaaca ttgatgtgcc taatattggt
    44281 cctgctagag ccattgctta tgatattgct gctgtgaata aagcgattga aatagctaag
    44341 aaaaacaaag atgaagctcc tattttttac attctagctt gtcgtatcgg gccttttata
    44401 gctagactta agaaaaagat tcaagctatc ggaggtactc tgtttgtcaa tccagatgga
    44461 catgagtggc ttcgggctaa atggagcctg ccggttcgaa agtattggaa attttcggaa
    44521 cagttgatgg tcaaatatgc agatttattg gtctgtgata gcaaaaatat tgaaaaatat
    44581 atccagaatg actataagca atatcaacca aagacgactt atattgctta cgggacggat
    44641 acaagtccgt ctatcctgaa atccgaagat ctaaaaattc gtagttggta tcaggaaaaa
    44701 ggacttagcg aaaatggcta ttatctagtg gtgggacgat tcgtccctga aaacaactac
    44761 gaaaccatga ttcgtgaatt tatcaaatct aagtccaaga aggactttgt cctcatcaca
    44821 aatgtggagc agaataaatt ttacgaccag ttgttacagg agactggttt tgacaaagat
    44881 cctagggtta aatttgtagg tactgtctat gaccaagaat tgcttaagta cattcgtgaa
    44941 aatgcttttg cctatttcca tgggcatgag gttggaggga ccaacccttc tctactagaa
    45001 gctctagcat ccacaaaact gaatttgttg ctagatgttg gttttaaccg tgaggttggt
    45061 gaagacgggg ctatttattg gagaaaagat aatcttcata aggttattga agagagtgaa
    45121 caaaaaacaa tagaagaaat aaaagaaata gatattctat ctacagagca ggtagaaaaa
    45181 agatttactt gggattttat tgttaatgaa tatgaaaatt tatttttact agggaaataa
    45241 ttatctaagt tacaaggatt ttatttgagg aagctttgat ggaagttcat cactttctta
    45301 gacaattatc taaataactt atgttattca tgaagttttt aaaatgatag atattctccc
    45361 tttttgcaaa gatactaaaa ttatagagag gaaatatgcc cagtagttga tagtgttttt
    45421 gggtagggtt aaacgacata gttttaagac aaactggtgg agatttaaga aataagtagt
    45481 tcttattaaa tcaaaaacgt gttcttattc tatattgtca tttagtcaaa ggtatcaagt
    45541 atgactatta aaattaatta tatgtttttt gtttgtctct cttttttcgg tatagtttta
    45601 agtagttcac aggtaatagt gaatctgggt ttgtccagta tagttcagta tatagcttat
    45661 tttttattgt tgctatgtat attttttacc ttaattaaaa atagtccgga tgtaatcgct
    45721 aatagaattg cttatt
//
LOCUS       DAVKWZ010000010        45662 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000010 DAVKWZ010000000
VERSION     DAVKWZ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 45662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45662
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.010"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            666..1118
                     /locus_tag="VW854_000615"
     CDS             666..1118
                     /locus_tag="VW854_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV0261896.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            1137..2474
                     /locus_tag="VW854_000616"
     CDS             1137..2474
                     /locus_tag="VW854_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEV0261897.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2553..3437
                     /locus_tag="VW854_000617"
     CDS             2553..3437
                     /locus_tag="VW854_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV0261898.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3437..4270
                     /locus_tag="VW854_000618"
     CDS             3437..4270
                     /locus_tag="VW854_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV0261899.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAIFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4288..6381
                     /gene="nanB"
                     /locus_tag="VW854_000619"
     CDS             4288..6381
                     /gene="nanB"
                     /locus_tag="VW854_000619"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEV0261900.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISSLLLDKLSGESQTVVMKFKADKPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSNIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLREKHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSKVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6393..7496
                     /locus_tag="VW854_000620"
     CDS             6393..7496
                     /locus_tag="VW854_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV0261901.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            7670..8368
                     /locus_tag="VW854_000621"
     CDS             7670..8368
                     /locus_tag="VW854_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEV0261902.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8387..9904
                     /locus_tag="VW854_000622"
     CDS             8387..9904
                     /locus_tag="VW854_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV0261903.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADLAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFLGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPFGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            10029..11357
                     /locus_tag="VW854_000623"
     CDS             10029..11357
                     /locus_tag="VW854_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV0261904.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11609..12475
                     /locus_tag="VW854_000624"
     CDS             11609..12475
                     /locus_tag="VW854_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV0261905.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            12491..13330
                     /locus_tag="VW854_000625"
     CDS             12491..13330
                     /locus_tag="VW854_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV0261906.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13603..14055
                     /locus_tag="VW854_000626"
     CDS             13603..14055
                     /locus_tag="VW854_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV0261907.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNQFEHHKNYADLHLLIEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            14152..14496
                     /locus_tag="VW854_000627"
     CDS             14152..14496
                     /locus_tag="VW854_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261908.1"
                     /translation="MVSSKYAARASFLDGQGITVDEMSWIINGIVNGLIGRYIKLGIY
                     AAKYGISMVRSILSRAAATAAARVGLLTKIFGWILRIAVLAADVYGNFANNIAAAWDA
                     YDKIPNNGRINF"
     gene            14773..15417
                     /locus_tag="VW854_000628"
     CDS             14773..15417
                     /locus_tag="VW854_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEV0261909.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSAFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            15586..16503
                     /locus_tag="VW854_000629"
     CDS             15586..16503
                     /locus_tag="VW854_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV0261910.1"
                     /translation="MSDLKKYEGVIPAFYACYDDQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTATHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKESFL"
     gene            16521..17405
                     /locus_tag="VW854_000630"
     CDS             16521..17405
                     /locus_tag="VW854_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV0261911.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17421..18272)
                     /locus_tag="VW854_000631"
     CDS             complement(17421..18272)
                     /locus_tag="VW854_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEV0261912.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            18472..20529
                     /gene="pbp2b"
                     /locus_tag="VW854_000632"
     CDS             18472..20529
                     /gene="pbp2b"
                     /locus_tag="VW854_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEV0261913.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTKRQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            20540..21136
                     /gene="recR"
                     /locus_tag="VW854_000633"
     CDS             20540..21136
                     /gene="recR"
                     /locus_tag="VW854_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEV0261914.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            21312..22355
                     /locus_tag="VW854_000634"
     CDS             21312..22355
                     /locus_tag="VW854_000634"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEV0261915.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLVYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPKLIERLVDL
                     AKESFDKREAHLI"
     gene            22439..23812
                     /locus_tag="VW854_000635"
     CDS             22439..23812
                     /locus_tag="VW854_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEV0261916.1"
                     /translation="MKLTIHEIAQVVGVKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLMP
                     ADPIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIHQAFQGLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSIQLHNQMILSLSPDVLDTVIFYGEDIAE
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAHDQILLKGSNSMNLAKLVE
                     SLENEDK"
     gene            23799..24410
                     /locus_tag="VW854_000636"
     CDS             23799..24410
                     /locus_tag="VW854_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV0261917.1"
                     /translation="MKTSDFVKYLQRMIAITDTGLTFTKDPFDRERYEDLRDLLSEML
                     NQASDLDSEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKLLDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIEILWQVYQGQREQYLD"
     gene            24501..25205
                     /locus_tag="VW854_000637"
     CDS             24501..25205
                     /locus_tag="VW854_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEV0261918.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            25424..26797
                     /gene="ftsA"
                     /locus_tag="VW854_000638"
     CDS             25424..26797
                     /gene="ftsA"
                     /locus_tag="VW854_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEV0261919.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            26814..28073
                     /gene="ftsZ"
                     /locus_tag="VW854_000639"
     CDS             26814..28073
                     /gene="ftsZ"
                     /locus_tag="VW854_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEV0261920.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            28078..28749
                     /locus_tag="VW854_000640"
     CDS             28078..28749
                     /locus_tag="VW854_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEV0261921.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDIIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKAAQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            28759..29298
                     /locus_tag="VW854_000641"
     CDS             28759..29298
                     /locus_tag="VW854_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEV0261922.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTSVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            29298..29561
                     /locus_tag="VW854_000642"
     CDS             29298..29561
                     /locus_tag="VW854_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEV0261923.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            29558..30343
                     /locus_tag="VW854_000643"
     CDS             29558..30343
                     /locus_tag="VW854_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEV0261924.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEYL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            30352..31140
                     /gene="divIVA"
                     /locus_tag="VW854_000644"
     CDS             30352..31140
                     /gene="divIVA"
                     /locus_tag="VW854_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEV0261925.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            31488..34280
                     /locus_tag="VW854_000645"
     CDS             31488..34280
                     /locus_tag="VW854_000645"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEV0261926.1"
                     /translation="MKLKDTLNLGKTEFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     QGKPHFTLHDGPPYANGNIHVGHAMNKISKDIIVRSKSMSGFYAPFIPGWDTHGLPIE
                     QVLSKQGVKRKEMDLVEYLKLCREYALSQVDKQREDFKRLGVSGDWENPYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGEARKFVVAAELLTSLSEK
                     FGWADVQVLETYRGQELNHIVTEHPWDTAVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKVKFHSEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMVAETIEHVAQLFEEHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVVVNRPELIYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDTVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFETEDFVQLSELPEAQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SELTIVEGPAPEAALSFEDVAFTVERATGEVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFAEAVAEGFEEK"
     gene            34683..35063
                     /locus_tag="VW854_000646"
     CDS             34683..35063
                     /locus_tag="VW854_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261927.1"
                     /translation="MKLKRLALISGIVGLVGGILLLIGPFVLLGTAVNTAATTLNGGA
                     TAGAFSGVALLLNALKIANLVLGIIAIVYYKGDKRVGAAPSVLMIVSGGVSLIPFLGW
                     VGGILAIIGGSLFLATLKKFKSEE"
     gene            35358..35546
                     /locus_tag="VW854_000647"
     CDS             35358..35546
                     /locus_tag="VW854_000647"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261928.1"
                     /translation="MENNDSFTKLKESTQKLFDAQKKRLNNEDRIETTKNNVIAKHCQ
                     TVLSFLVLTSFFVKNCVK"
     gene            35597..36289
                     /locus_tag="VW854_000648"
     CDS             35597..36289
                     /locus_tag="VW854_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEV0261929.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIKFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            36342..38753
                     /locus_tag="VW854_000649"
     CDS             36342..38753
                     /locus_tag="VW854_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEV0261930.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYGFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALATTSYVYKGTRFE
                     RKAFASFPDDLLIQCFTKEGLETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLETVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAARASLNDRGDGGTGWSEANKINLWARLGDGNRAHKLLAEQLKTSTLQNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            39003..39704
                     /locus_tag="VW854_000650"
     CDS             39003..39704
                     /locus_tag="VW854_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0261931.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNSDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIHMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            39706..42414
                     /locus_tag="VW854_000651"
     CDS             39706..42414
                     /locus_tag="VW854_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV0261932.1"
                     /translation="MIKRKTYWKDLIQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEDLEQTLSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIIAKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLAFVLSLLASVLPAYLVAWRELHDEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKAL
                     YKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLIAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(42510..43001)
                     /gene="tpx"
                     /locus_tag="VW854_000652"
     CDS             complement(42510..43001)
                     /gene="tpx"
                     /locus_tag="VW854_000652"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEV0261933.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(43124..44053)
                     /gene="psaA"
                     /locus_tag="VW854_000653"
     CDS             complement(43124..44053)
                     /gene="psaA"
                     /locus_tag="VW854_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEV0261934.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(44085..44933)
                     /locus_tag="VW854_000654"
     CDS             complement(44085..44933)
                     /locus_tag="VW854_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV0261935.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(44930..45652)
                     /locus_tag="VW854_000655"
     CDS             complement(44930..45652)
                     /locus_tag="VW854_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV0261936.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
BASE COUNT        13923 a         8141 c        10020 g        13578 t
ORIGIN      
        1 acttttaata tacttgatat agttaaaaaa gatttgaaac taaattccaa attagaaaaa
       61 gacttgaaat actaaaaaaa aaaagtatac tctaattgaa aacggtaaca aaactaattt
      121 agagaatgaa atatagagta tttctctctt aaaagttttt ggtgaaacga gatgtagaaa
      181 ggagatttag ccaaagagtc tattagtgct agaataatag attagaatta ttttagaaaa
      241 acgaagtgag cagcttataa attcaagtcc ccaaatagat tcatactagt atcttttgca
      301 aaaaataaag ggcgacttcc ttcatgaata tcaatttcat ctataaggaa ggtagctaat
      361 tgaactaact tatttattct gtttgttgct agaaaaatca gacctccttg tgaagattga
      421 ggagatactt aatgaaaatc aaagaagaaa ctagcaagct agtagcagat tgcccaaaac
      481 accgctttga ggttgtagat aagactgacg tatataatcc aaggtgaagc gactgtggtt
      541 tgaagagatt ttcaaagagt ataggctaga gagtagtgtt tttatgtcct tctagtagaa
      601 aatgctagac agaagaatgg ggaacttgga taggaaaaat agattgagaa aggaggttag
      661 aagagatgat tattacaaaa attagccgtt taggaactta tgtgggagta aatccacatt
      721 ttgcaacatt aatagatttt ctagaaaaaa caggactaga aaatttaaca gaaggttcga
      781 ttgctatcga tggtaatcga ttgtttggga attgctttac ttatctagca gatggtcaag
      841 caggggcttt ctttgaaaca caccaaaaat atttggatat tcatttagtt ttggaaaacg
      901 aagaagccat ggctgttaca tcgccggaaa atgtaagcgt tacccaagaa tatgatgaag
      961 agaaagatat tgaattatac acagggaaag tggaacagtt ggttcatttg agagctggtg
     1021 aatgcctcat cacttttcca gaagatttac atcaacccaa ggttcgtata aatgatgaac
     1081 ctgtgaaaaa agttgtcttt aaagttgcga tttcttaatg tagaaagaga agaacgatga
     1141 aaaaaatgag aaagttttta tgtctagctg gaattgcgct agcggctgtt gccttggtag
     1201 cttgttcagg aaaaaaagaa gctacaacta gtactgaacc accaacagaa ttatctggtg
     1261 agattacaat gtggcactcc tttactcaag gaccccgttt agaaagtatt caaaaatcag
     1321 cagatgcttt catgcaaaag catccaaaaa cgaaaatcaa gattgaaaca ttttcttgga
     1381 atgacttcta tactaaatgg actacaggtt tagcaaatgg aaatgtgcca gatatcagta
     1441 cagctcttcc taaccaagta atggaaatgg tcaactcaga cgctttggtt ccgctaaatg
     1501 attctatcaa gcgtattgga caagataaat ttaacgaaac tgccttaaat gaagcaaaaa
     1561 tcggagatga ttactactct gttcctcttt attcacatgc acaagtcatg tgggttagaa
     1621 cagatttgtt aaaagaacat aatattgagg ttcctaaaac ttgggatcaa ctctatgaag
     1681 cttctaaaaa attgaaagaa gctggagttt atggcttgtc tgttccgttt ggaacaaatg
     1741 acttaatggc aacacgtttc ttgaacttct acgtacgtag tggtggagga agcctcttaa
     1801 caaaagatct taaagcagac ttgacaagcc aacttgctca agatggtatt aaatactggg
     1861 ttaaattgta taaagaaatc tcacctcaag attctttgaa ctttaatgtc cttcaacaag
     1921 ctaccttgtt ctatcaagga aaaacagcat ttgactttaa ctctggcttc catatcggag
     1981 gaattaatgc caacagtcct caattgattg attcgattga tgcttatcct attccaaaaa
     2041 tcaaagagtc tgataaagat caaggaattg aaacctcaaa cattccaatg gttgtttgga
     2101 aaaattcaaa acatccagaa gttgctaaag cattcttaga agcactttat aatgaagaag
     2161 actacgttaa attccttgat tcaactccag taggtatgtt gccaactatt aaggggatta
     2221 gcgattctgc agcctataaa gaaaatgaaa ctcgtaagaa atttaaacat gctgaagaag
     2281 taattactga agctgttaaa aaaggtactg ctattggtta tgaaaatgga ccaagtgtac
     2341 aagctggtat gttaactaac caacacatta ttgaacaaat gttccaagat atcattacaa
     2401 atggaacaga tcctatgaaa gcagcaaaag aagcagaaaa acaattaaat gatttatttg
     2461 aggctgttca gtagatgtaa aagactagaa aataggtggg ctagtgagct gaaaagctct
     2521 agcccaatct tgtaaaagaa gggagaagga gaatggttaa agaacgtaat ttaactcgct
     2581 ggatatttgt tttgccagct atgattatcg taggattact ctttgtttat ccgtttttct
     2641 cgagtatttt ttatagcttt accaataagc atttgattat gcctaattat aaatttgttg
     2701 gtttggctaa ctataaagct gtgctatcag atcccaactt ctttaatgcg ttctttaatt
     2761 caattaagtg gaccgttttc tcattagttg gtcaagtttt agtagggttt gtattggctt
     2821 tagctcttca cagagtacgc cacttcaaga aattatatag gaccttattg attgttcctt
     2881 gggcatttcc taccatcgtt attgcctttt cttggcagtg gattctaaac ggggtttatg
     2941 gctacttacc taatctaatc gtaaaattag gtttaatgga acatacacct gcatttttga
     3001 cagatagtac atgggcattc ctatgtttgg tgtttatcaa catttggttt ggagcaccaa
     3061 tgattatggt taatgtgctt tcagctttgc aaacagtacc agaagaacaa tttgaggctg
     3121 ctaagataga tggtgcttca agttggcagg tgttcaagtt tatcgtcttt ccacatatta
     3181 aagtggttgt aggacttcta gttgttttga gaactgtatg gatctttaat aactttgaca
     3241 ttatctacct cattactggt ggtggaccag ccaatgctac aacgacgctt ccaatttttg
     3301 cttacaacct gggttgggga actaaattgt tgggtcgtgc ttcagcagtt acagtattgc
     3361 tctttatctt cttggtggcg atttgcttta tctactttgc tatcatcagt aagtgggaaa
     3421 aggagggtag aaaataatga agaagaaatc cagtatttat ttagatattc tctcacatgt
     3481 acttttagtt ggtgcgacca tcgttgcaat tttcccattg gtatggatta tcatatcttc
     3541 tgtcaaaggg aaaggggaat taactcagta tccaacacga ttttggcctg aacagtttac
     3601 attagattat ttcactcatg ttatcaacga tttgcacttc attgataaca ttcgaaacag
     3661 tttaatcatt gccttggcta caaccctcat tgcgattatt atttctgcta tggcagccta
     3721 tggtattgtt cgattctttc ctaaattggg agcaatcatg tcgagactac tcgtcattac
     3781 ctacattttc ccaccaattt tgttagcaat tccctattca attgccattg ctaaagttgg
     3841 gttaacaaat agtttatttg gcttgatgat ggtttatcta tcttttagtg ttccatatgc
     3901 agtttggctc ttagttggat ttttccaaac agttccaatt ggaattgaag aagcggctag
     3961 aattgatggt gcaaataaat ttgttacgtt ttataaagtt gtgctaccga ttgtagcacc
     4021 aggtattgta gcaacagcta tttatacatt tatcaatgct tggaatgaat tcctgtatgc
     4081 cttgattttg attaacaata caggaaagat gacagtagca gtagcccttc gttcacttaa
     4141 tggttcagaa atactagact ggggagatat gatggcagcg tctgttattg tagttcttcc
     4201 atcaattatt ttcttctcta tcatccaaaa taagattgca agtggattat cagaaggatc
     4261 tgtgaagtag acgaaagaag gaaaaaaatg aataaaagag gtctttattc aaaactagga
     4321 atttctgttg taggcattag tcttttaatg ggagttccca ctttgattca tgcgaatgaa
     4381 ttaaactatg gtcaactgtc catatctcct atttttcaag gaggttcata tcaactgaac
     4441 aataagagta tagatatcag ctctttgtta ttagataaat tgtctggaga gagtcagaca
     4501 gtagtaatga aatttaaagc agataaacca aactctcttc aagctttgtt tggcctatct
     4561 aatagtaaag caggctttaa aaataattac ttttcaattt tcatgagaga ttctggtgag
     4621 ataggtgtag aaataagaga cgcccaaaag ggaataaatt atttattttc tagaccagct
     4681 tcattatggg gaaagcataa aggacaggca gttgaaaata cactagtatt tgtatctgat
     4741 tctaaagata aaacatacac aatgtatgtt aatggaatag aagtgttctc tgaaacagtt
     4801 gatacatttt tgccaatttc aaatataaat ggtatagata aggcaacact aggagctgtt
     4861 aatcgtgaag gcaaggaaca ttacctcgca aaaggaagta ttgatgaaat cagtctattt
     4921 aacaaagcaa ttagtgatca ggaagtttca aatattccct tgtcaaatcc atttcagtta
     4981 attttccaat caggagattc tactcaagct aactatttta gaataccgac actatataca
     5041 ttaagtagtg gaagagttct atcaagtatt gatgcacgtt atggtgggac tcatgattct
     5101 aaaagtaaga ttaatattgc cacttcttat agtgatgata atgggaaaac gtggagtgag
     5161 ccaatttttg ctatgaagtt taatgactat gaggagcagt tagtttactg gccacgagat
     5221 aataaattaa agaatagtca aattagtgga agtgcttcat tcatagattc atccattgtt
     5281 gaagataaaa aatctgggaa aacgatatta ctagctgatg ttatgcctgc gggtattgga
     5341 aataataatg caaataaagc cgactcaggt tttaaagaaa taaatggtca ttattattta
     5401 aaactaaaga agaatggaga taacgatttc cgttatacag ttagagaaaa tggtgtcgtt
     5461 tatgatgaaa caactaataa acctacaaat tatactataa atgataagta tgaagttttg
     5521 gagggaggaa agtctttaac agtcgaacaa tattcggttg attttgatag tggctcttta
     5581 agagaaaaac ataatggaaa acaggtccct atgaatgttt tctacaaaga ttcgttattt
     5641 aaagtgactc ctactaatta tatagcaatg acaactagtc agaatagagg agagagttgg
     5701 gaacaattta agttgttgcc tccgttctta ggagaaaaac ataatggaac ttacttatgt
     5761 cccggacaag gtttagcatt aaaatcaagt aacagattga tttttgcaac atatactagt
     5821 ggagaactaa cctatctcat ttctgatgat agtggtcaaa catggaagaa atcctcagct
     5881 tcaattccgt ttaaaaatgc aacagcagaa gcacaaatgg ttgaactgag agatggtgtg
     5941 attagaacat tctttagaac cactacaggt aagatagctt atatgactag tagagattct
     6001 ggagaaacat ggtcgaaagt ttcgtatatt gatggaatcc aacaaacttc atatggcaca
     6061 caagtatctg caattaaata ctctcaatta attgatggaa aagaagcagt cattttgagt
     6121 acaccaaatt ctagaagtgg ccgtaaggga ggtcaattag ttgtcggttt agtcaataaa
     6181 gaagatgata gtattgattg gaaataccac tatgatattg atttgccttc gtatggttat
     6241 gcctattctg cgattacaga attgccaaat catcacatag gtgtactgtt tgaaaaatat
     6301 gattcgtggt cgagaaatga attgcattta agcaatgtag ttcagtatat agatttggaa
     6361 attaatgatt taacaaaata aaggagaaaa acatggttaa atacggtgtt gttggagcag
     6421 ggtattttgg agctgaattg gctcgctaca tgcaaaagaa tgatggagca gagattactc
     6481 ttctctatga tccagataat gcagaggcga ttgcagaaga attgggagca aaagtagcaa
     6541 gttccttaga tgagttggtt tctagcgatg aagtagattg tgttatcgtc gcaactccaa
     6601 ataatcttca taaggaaccg gttattaagg ctgcacagca tggtaaaaat gttttctgtg
     6661 aaaaaccaat tgcgctttct tatcaagatt gtcgcgagat ggtagatgcg tgtaaagaaa
     6721 acaatgtaac ctttatggca ggacatatta tgaatttctt taatggtgtt catcatgcaa
     6781 aagaactcat taatcaagga gttatcggag acgttctata ttgtcataca gctcgtaatg
     6841 gttgggaaga acaacaaccg tcagtatcat ggaaaaaaat tcgtgaaaaa tcaggtggtc
     6901 acttgtatca ccacatccat gaattggatt gcgttcaatt ccttatgggg ggcatgcctg
     6961 aaactgtaac catgacaggt ggaaatgtgg cccatgaagg tgaacatttc ggtgatgaag
     7021 atgatatgat ttttgtcaat atggaatttt ctaataagcg ttttgctttg ttagaatggg
     7081 gttcagctta tcgttggggt gaacattatg tcttaatcca aggaagcaaa ggtgccatcc
     7141 gcttagactt attcaactgt aaaggaactc ttaagctaga tgggcaagaa agctatttct
     7201 tgattcacga atcgcaagaa gaagatgatg atcggactcg tatctatcat agtacagaga
     7261 tggatggagc aattgcttat ggtaaaccag gtaaacgtac tccattatgg ctatcatctg
     7321 tcattgataa agaaatgcgc tatctgcatg agattatgga aggagctcca gtatcagaag
     7381 aatttgcaaa acttttgaca ggtgaagctg ccctagaagc aattgctact gcagatgctt
     7441 gtacccagtc tatgtttgaa gatcgcaaag taaaattgtc agaaattgta aaataaattt
     7501 tggtattctc ctatttatag gtcgacttgc tcctctgaaa gtacttttag aggagctgtt
     7561 tgactttgct agtttttgaa actgaaatct attatactac aaactattga aagcgtttta
     7621 attttaaggt ataataatct catagaaata aagaaaagga ggaaagagga tgccacagat
     7681 tagcaaagaa gccttgattg agcaaatcaa agatggaatc atcgtttctt gtcaggctct
     7741 tcctcatgaa ccgctttata cagaagcggg aggggtgatt cccttgctgg tcaaagcggc
     7801 tgagcaaggt ggagcagtcg gtatccgagc aaacagtgtt cgcgatatca aggaaattaa
     7861 ggaagtcact aaacttccaa tcattgggat tatcaaacgt gattatccac ctcaggaacc
     7921 cttcatcacg gctactatga aagaagttga tgaattggca gaactggaca tcgaggtgat
     7981 tgctctggat tgtaccaagc gtgaacgcta cgatggtttg gaaattcaag agttcattcg
     8041 tcaggttaag gagaaatatc ctaatcagct tttgatggct gatactagta tcttcgaaga
     8101 agggctagca gctgtagaag caggaattga ctttgtcgga acaaccttat caggctacac
     8161 atcctacagt ccaaaagtag acggtccaga ttttgaattg attaagaaac tctgtgatgc
     8221 cggtgtagat gtcattgcag aaggaaaaat tcatacacca gaacaagcca aacaaatcct
     8281 tgaatatgga gtgcgaggca tcgttgttgg tggcgccatt actagaccaa aagagattac
     8341 agaacgcttc gttgctagtc ttaaataaga tgtgaggggg agttttatgt ttaaagtttt
     8401 acaaaaagtt ggaaaagctt ttatgttacc tatagctata cttcctgcag caggtctact
     8461 tttggggatt ggtggtgcac tttcaaaccc aaccacgata gcaacttatc caatactaga
     8521 caatagtatt tttcaatcaa tattccaagt aatgagctct gcaggagagg ttgtattcag
     8581 taatttgtca ctacttctct gtgtgggatt atgtattggc ttagcgaaac gagataaagg
     8641 aaccgctgcg ttagcaggag taactggtta cttagttatg actgcaacga tcaaagcttt
     8701 agtaaaactt tttatggcag aaggatctgc aattgatact ggagttattg gagcattagt
     8761 tgtcggaata gttgccgtat atttgcacaa ccgatataac aatattcaat taccttccgc
     8821 tttaggattc tttggaggtt cacgcttcgt tcctattgtt acatcgttct cttctatctt
     8881 gattggcttt gtcttctttg ttatttggcc acctttccaa caacttcttg tttctacagg
     8941 tggatatatt tctcaggcgg gtccaattgg aacttttcta tatggatttt taatgagact
     9001 ttctggagca gtaggcttac atcatataat ttaccctatg ttttggtata ctgaacttgg
     9061 tggtgttgaa actgttgcag gacaaacagt ggttggagct caaaaaatat tttttgctca
     9121 attagccgat ttggcccatt ctggattatt tacagaagga acaaggtttt ttgcaggtcg
     9181 tttctcaaca atgatgttcg gtttaccggc tgcctgttta gcgatgtacc atagtgttcc
     9241 taaaaatcgt cgtaaaaaat acgcgggttt gtttttggga gttgctttaa catcttttat
     9301 taccggtatt acagaaccaa ttgaatttat gtttctattc gtcagtccgg ttctatatgt
     9361 tgttcacgca ttccttgatg gtgttagctt ctttattgca gacgtcttaa atatttcaat
     9421 aggaaacaca ttttcaggag gtgtaatcga tttcacatta tttggaattt tgcaggggaa
     9481 cgctaagacg aattgggttc ttcagattcc atttggactt atttggagtg ttttgtatta
     9541 tattattttt agatggttca ttactcaatt caacgttcta acgccaggac gaggagaaga
     9601 agtagattct aaagaaattt ctgaatccgc agattcgact tcaaatactg cagattattt
     9661 aaaacaggat agcctacaaa ttatcagagc cttgggtgga tcaaataata tagaagatgt
     9721 agatgcttgt gtgacacgtt tacgtgtagc tgtaaaagaa gttaatcaag ttgataaagc
     9781 acttttaaaa caaattggtg cagttgatgt cttagaagtg aagggtggca ttcaagcaat
     9841 ctatggagca aaagcaatct tatataaaaa tagtattaat gaaattttag gtgtagatga
     9901 ttaagtactt actgacttaa taaaaaacag aggagagtga tggatgagta ggatgaaatg
     9961 aaatcgcata caagaaataa agaactcatt atccaagttg gatacgctta ttacatagga
    10021 gaatacaaat gaaatttaga aaattagctt gtacagtact tgcgggtgct gcggttcttg
    10081 gtcttgctgc ttgtggcaat tctggcggaa gtaaagatgc tgccaaatca ggtggtgacg
    10141 gtgccaaaac agaaatcact tggtgggcat tcccagtatt tacccaagaa aaaactggtg
    10201 acggtgttgg aacttatgaa aaatcaatca tcgaagcgtt tgaaaaagca aacccagata
    10261 taaaagtgaa attggaaacc atcgacttca agtcaggtcc tgaaaaaatc acaacagcca
    10321 tcgaagcagg aacagctcca gacgtactct ttgatgcacc aggacgtatc atccaatacg
    10381 gtaaaaacgg taaattggct gagttgaacg acctcttcac agatgaattt gttaaagatg
    10441 tcaacaatga aaacatcgta caagcaagta aagctggaga caaggcttat atgtatccga
    10501 ttagttctgc cccattctac atggcaatga acaagaaaat gttagaagat gctggagtag
    10561 caaaccttgt aaaagaaggt tggacaactg atgattttga aaaagtattg aaagcactta
    10621 aagacaaggg ttacacacca ggttcattgt tcagttctgg tcaaggggga gaccaaggaa
    10681 cacgtgcctt tatctctaac ctttatagcg gttctgtaac agatgaaaaa gttagcaaat
    10741 atacaactga tgatcctaaa ttcgtcaaag gtcttgaaaa agcaactagc tggattaaag
    10801 acaatttgat caataatggt tcacaatttg acggtggggc agatatccaa aactttgcca
    10861 acggtcaaac atcttacaca atcctttggg caccagctca aaatggtatc caagctaaac
    10921 ttttagaagc aagtaaggta gaagtggtag aagtgccatt cccatcagac gaaggtaagc
    10981 cagctcttga ataccttgta aacgggtttg cagtattcaa caataaagac gacaagaaag
    11041 tcgctgcatc taagaaattc atccagttta tcgcagatga caaggagtgg ggacctaaag
    11101 acgtagttcg tacaggtgct ttcccagtcc gtacttcatt tggaaaactt tatgaagaca
    11161 aacgcatgga aacaatcagc ggctggactc aatactactc accatactac aacactattg
    11221 atggatttgc tgaaatgaga acactttggt tcccaatgtt gcaatctgta tcaaatggtg
    11281 acgaaaaacc agcagatgct ttgaaagcct tcactgaaaa agcaaacgaa acaatcaaaa
    11341 aagctatgaa acaatagtcc ttagttattc tataaaaagt agttttttaa agaacctaag
    11401 agtgtatacc cccttttccc tctacacaga tagtgtaaga aaagggggct tttgtttaaa
    11461 atgtaagaaa ctgtcacgaa attaaaatga agttcttaca taagcgaatc ataaaaaatt
    11521 tcattttgat tttaaaacag ttcaagaaag tcaaaaaatt attctatttg aaagagaggt
    11581 gccgactgtg aaagtcaata aaatccgtat gcgggaaaca gtgatttcct acgctttcct
    11641 agcaccagta ttattcttct ttgtcatctt tgtgttggct ccgatggtga tgggcttcat
    11701 tacaagtttc tttaactact caatgactaa atttgagttt gtaggcttgg ataactatat
    11761 ccgtatgttt aaagatcctg tctttacaaa atctctgatt aacacagtta ttttggttat
    11821 tggatctgta ccagttgttg ttctattctc actctttgta gcatctcaga cctatcatca
    11881 aaatgtcatt gccagatcct tctaccgttt cgtcttcttc cttcctgttg taacgggtag
    11941 tgttgccgtg acagttgttt ggaaatggat ttatgaccca ctatcaggga ttctaaactt
    12001 tgtccttaag tcaagccaca tcatcagcca aaacatttct tggttgggag ataaaaactg
    12061 ggcattgatg gcgattatga ttattctctt gaccacttca gttggtcagc ccatcatcct
    12121 ttatatcgct gccatgggga atattgacaa ttcactggtt gaagcggcgc gtgttgatgg
    12181 tgcaactgag tttcaagttt tttggaagat taaatggcca agccttcttc caacaactct
    12241 ttatattgca atcatcacaa caattaactc attccagtgt ttcgccttga ttcagctttt
    12301 gacatctggt ggtccaaact actcaacaag taccttgatg tactaccttt acgaaaaagc
    12361 cttccaattg acagaatacg gctatgccaa cacaattggt gtcttcttgg cagtcatgat
    12421 tgctatcgta agctttgttc aatttaaagt acttggaaac gacgtagaat actaaagaaa
    12481 ggagacagct atgcaatcta cagaaaaaaa accattaaca gcctttactg ttatttcaac
    12541 aatcattttg ctcttgttga ctgtgctgtt catctttcca ttctactgga ttttgacagg
    12601 ggcattcaaa tcacaacctg atacaattgt tattcctcct cagtggttcc ctaaaatgcc
    12661 aaccatggaa aacttccaac aactcatggt gcagaaccct gccttgcaat ggatgtggaa
    12721 ctcagtattt atctcattgg taaccatgtt cttagtttgt gcaacctcat ctctagcagg
    12781 ttatgtattg gctaaaaaac gtttctatgg tcaacgcatt ctatttgcta tctttatcgc
    12841 tgctatggcg cttccaaaac aagttgtcct tgtaccattg gtacgtatcg tcaacttcat
    12901 gggaatccat gatactctct gggcagttat cttgcctttg attggatggc cattcggtgt
    12961 cttcctcatg aaacagttca gtgaaaatat ccctacagag ttgcttgaat cagctaaaat
    13021 cgacggttgt ggtgagattc gtaccttctg gagtgtagcc ttcccgattg tgaaaccagg
    13081 gtttgcagcc cttgcaatct ttaccttcat caatacttgg aatgactact tcatgcaatt
    13141 ggtaatgttg acttcacgta acaatttgac catctcactt ggggttgcga ccatgcaggc
    13201 tgaaatggca accaactatg gtttgattat ggcaggagct gcccttgctg ctgttccaat
    13261 cgtcacagtc ttcctagtct tccaaaaatc cttcacacag ggtattacta tgggagcggt
    13321 caaaggataa tactctgcga aaatcgaatg caaactacgt cagcttcacc ttgccatact
    13381 taagtattgc ctgcggttag cttcctagtt tgttcttcaa ttttcattga gtataggaaa
    13441 atcaatctat caagatacag aagtatattt tatagattta gagaatatag aggttataag
    13501 tgtctacaaa atggagggta tgcagttact ttatgaagtt ttgtcagaca cttatcaact
    13561 taagaatggt tttagttaac tatcagaaac gaaggaaaga gtatgatttt tgacgatttg
    13621 aaaaacatca ccttttacaa agggattcat cccaatttag acaaggctat cgactacctc
    13681 taccaacatc gtaaagattc attcgaatta ggaaagtatg agattgatgg agataaagtc
    13741 tttctagttg ttcaggaaaa tgtcctcaat caagttgaga ataatcaatt tgaacaccat
    13801 aagaactatg cagatttgca tttgctgata gaagggcatg aatattcgag ctacggttca
    13861 cgtatcaaag acgaggcagt agcattcgac gaagcgagtg acattggctt tgttcattgt
    13921 catgaacact acccactctt attgggttat cacaattttg cgattttctt cccaggtgag
    13981 ccacatcagc caaatggtta tgcaggcatg gaagaaaagg ttcgaaaata tctctttaaa
    14041 attttgattg attaaaaata ggatgaattg tttttttgta aagctttgac aatactctac
    14101 catgaaattg atctttgtga ggtagagaaa tgagaataaa atatttaaaa attggtatct
    14161 tctaagtatg ctgcaagagc cagtttctta gatggacagg ggattacagt tgatgagatg
    14221 tcctggataa ttaatggcat tgtgaatgga ttgattggta gatacataaa attaggtatt
    14281 tatgcagcca agtatggtat tagtatggtg cgatcgatct taagtagggc agctgcaact
    14341 gcggcagcaa gagtaggatt attgaccaag atttttggat ggattttacg aatagctgtg
    14401 ctcgcagctg atgtatatgg taattttgcc aacaatattg ctgcagcttg ggatgcatat
    14461 gataaaattc ctaacaatgg tcgtataaac ttttaaaatg cgagaatgaa agcactttgt
    14521 atttttttat tgaatatgtt agcttggaca gtgcttgcaa tgataattcg tggagggcta
    14581 gatggatttg ataggcatac ttggagtact attttaattg cttcgctgtc cgggatatat
    14641 gattataagc ccatagataa aaatagaaaa aagtccaaaa gaaaaaatag atttgttcat
    14701 ggtagggact tatgaaagct ttactgacaa aaaagaaaac agtttacaaa gaaaaatgat
    14761 ggaggagcaa acatggcaca aaaaggagta agccttatca aggcagcatt tgatacagat
    14821 aactttctca tgcgttttag tgagaaggtc ttggacatcg tgacagccaa tcttcttttt
    14881 gtcgtctctt gtttacccat cgtgacgatt ggagtggcta aaatcagcct ctacgagacc
    14941 atgttcgaag ttaagaagag cagacgggtg cctgttttta aaatctatct aagatctttc
    15001 aagcaaaatc tgaaactagg tcttcagctg ggtttaatgg agttaggaat tgtgtttctt
    15061 accctttcag atctctatct tttctggggt caaacagctc tgcccttcca attgctgaaa
    15121 gccatttgtt taggtattct gatttttctt actatcgtga tgctggctag ttaccctatc
    15181 gcggcacgtt atgacctatc ttggaaagaa attcttcaaa aaggattgat gttggctagt
    15241 tttaactttc cttggttctt cctcatgtta gccattcttg tcctcattgt gatggttctt
    15301 tatctgtccg ccttcagtct actcttaggt ggctcagcct tcctactttt tgggtttgga
    15361 ctattggtct ttatccagac tggattgatg gagaaaattt tcgcaaaata ccaataggag
    15421 ctttatttct gaaactactt tcaaaggctc caaacgctat tctataagcg agaaactaaa
    15481 atctaagtgt ctataagata ttgaaagcct tttcggtaaa gtgtatacta aagaagtaaa
    15541 gaaaaatctg ctttagcaga aaataaaaat aataggagaa aatctatgtc agatttgaaa
    15601 aaatacgaag gtgtcattcc agccttctac gcatgttatg atgatcaagg agaagtaagc
    15661 ccagaacgta cgcgtgcctt ggttcaatac ttcattgata aaggtgttca aggtctttat
    15721 gtcaatggtt cttctggtga atgtatctac caaagcgttg aagatcgcaa gttgattttg
    15781 gaagaagtca tggcggtagc taaaggtaaa ttgaccatta ttgcccatgt tgcctgcaac
    15841 aatactaaag acagtatgga acttgctcgc catgctgaaa gcttgggagt agatgctatt
    15901 gcaacgattc caccaattta tttccgcttg ccagaatact cagttgccaa atactggaac
    15961 gatatcagtt ctgcagctcc aaacacagac tacgtgattt acaacattcc tcaattggca
    16021 ggggttgctt tgactccaag cctttacaca gaaatgttga aaaatcctcg tgttatcggt
    16081 gtgaagaact cttctatgcc agttcaagat atccaaacct ttgtgagcct tggtggggaa
    16141 gaccatatcg tctttaacgg tccagatgaa cagttcctag gtggtcgcct catgggtgcc
    16201 aaagctggta tcggtggtac ttatggtgct atgccagaac tcttcttgaa actcaaccaa
    16261 ttgattgcgg ataaagattt agaaacagcg cgtgagttac agtatgctat caacgctatc
    16321 attggtaaat tgactgctac acatggaaat atgtacggtg tcatcaaaga agttttgaag
    16381 atcaatgaag gcttgaatat tggttctgtt cgttcaccat tgacaccagt gactgaagaa
    16441 gatcgtccag ttgtagaagc ggctgctgcc ttgattcgtg aaaccaagga gagcttcctc
    16501 taatctaaaa ggaggtattt atgacatact acgttgcaat tgatatcggt ggaaccaaca
    16561 tcaagtatgg tttggttgat caagaggggc aacttcttga atcgcatgaa atgccaactg
    16621 aggcgcataa gggtggacct catatcttac aaaagaccaa agatatcgta gctagttatt
    16681 tagaaaaagg cccagtagca ggtgttgcca tatcttctgc tgggatggtg gatccggata
    16741 agggtgagat tttctatgct gggccgcaaa tccctaacta cgcaggcacc cagttcaaaa
    16801 aggaaatcga agaaagcttt actattcctt gtgagattga aaatgatgtc aactgtgcag
    16861 gtcttgctga ggcagtatct ggttcaggca agggagcaag tgtgacactt tgcttgacca
    16921 ttggaacagg tatcggtggt tgcttgatta tggataggaa agtcttccat ggttttagca
    16981 attcagcctg tgaagtcggg tatatgcata tgcaggatgg agcttttcaa gacttggctt
    17041 ctacaacagc tttagtgaaa tatgtagctg aagcccatgg agaagatgtt gatcagtgga
    17101 atggccgacg tatctttaag gaagccactg aaggaaacaa aatctgcatg gaaggtattg
    17161 accgtatggt tgactatcta ggaaaaggtc tggcaaatat ttgctacgtt gccaatccag
    17221 aagtggttat tcttggtggt ggtatcatgg ggcaagaggc tatcctcaaa cctaagatcc
    17281 gcacagcctt gaaagaggct ttggtaccaa gtttagcaga aaaaacacga ttagaatttg
    17341 cccatcacca aaatacagca gggatgttgg gtgcatatta tcattttaag acaaaacaat
    17401 cctagtttgg ctcagccaaa ctaggatttt cttacacgtt tttgtctacg atagccgttg
    17461 agttttttat tttcccagta gctattaaag attttttcct tgctttcgcg attgatttcc
    17521 aaaaagtagg cataaatcaa atcgataaag aagagcatag gaagttgagc ggatattcgt
    17581 tggatatagg agggttggct gtgggtggct acaagaacag tctctgtata ggtctggcta
    17641 tctttattgg gaacacttgt aaagagtaca gtctttgccc ccatctcctt ggcatctaat
    17701 agactatcta aaatagaagg agttgagcct gaaagtgaga agcccagtac tagacaattt
    17761 tcatccatga tgctggttgt ccaggcaaag ccgtcttggt ctgtcaaagc ttcgcagacc
    17821 acacctagtc gcataaaacg taatttcatt tcacgggcga cgaggccaga actccctgtt
    17881 ccaaagaagt agatacgctc agcatcttcg attagctggg caattcgctc tagttggatt
    17941 tcgtcaatca agtcttgtgt ttgttccctc atattgctat aacttctgag gactcgtttg
    18001 gtcagtggac tgtgcttgga gacttggttg gcttgatttt ctgcctgatg ttggtattgg
    18061 aaaataaatt ctcggtagcc agtaaagcca cactttttag caaagcgggt caaagcagct
    18121 tgagaaatat gtaatttttg ggtgacttgt tgagaagata aatcatctgt aatcgtttca
    18181 gcttgcaaaa aatagcgagc gatttcttgc tctaggtctg tcatttcttc aaaatgtgaa
    18241 tcaatgatag ttgcgatatc tggtttgtcc atagggaaag ctcctttaca tgagtcatac
    18301 tggaagacta gatcagggaa tagtcacact tcattataac acataatata aggatagata
    18361 aataaaaacg catctctgtt ttaaaaacga aaaaatcgaa aaagcttctc tcttttccat
    18421 aattttctac tcaaattgtg gtacaattaa gagtaagatt ttaagttaga aatgagactg
    18481 atttgtatga gaaaatttaa cagccattcg attccgattc ggcttaattt attgttttca
    18541 atcgtcattt tactctttat gaccattatt ggtcgtttgt tgtatatgca ggttttgaac
    18601 aaggattttt acgaaaaaaa gctagcctca gctagtcaga ccaagattac aagcagttca
    18661 gcccgtgggg aaatttatga tgctagtgga aaacctttgg tagaaaatac gttaaagcag
    18721 gttgtttcct ttacgcgtag caataaaatg acggctacag acttaaaaga aacagctaaa
    18781 aagttactga cttatgtgag catcagttct ccaaatttga caaaacgcca gctggcggat
    18841 tactatttgg ctgatcctga aatctataaa aaaacagtgg aagctctccc aagcgagaaa
    18901 cgcttggatt cagatggcaa tcgtctatcc gaatcagaac tgtataacaa tgcggtcgat
    18961 agtgtccaaa caagtcaact aaactataca gaggatgaaa agaaagaaat ctatcttttt
    19021 agtcagttaa atgctgttgg aaactttgcg acaggaacca ttgctacaga tcctctaaat
    19081 gattctcagg tggctgttat tgcctctatt tcaaaggaga tgcctggcat tagtatttct
    19141 acttcttggg atagaaaggt tttggaaact tccctttctt ctatagttgg gagtgtatcc
    19201 agtgaaaaag ctggtctccc agcggaagaa gcagaagcct atcttaaaaa aggctattct
    19261 ctaaatgacc gtgtaggaac ctcctatttg gaaaagcaat atgaagagac cttacaagga
    19321 aaacgctcgg taaaagaaat ccatctggat aaatatggca atatggaaag cgtggataca
    19381 attgaggaag gtagtaaggg aaacaatatc aaactgacca ttgatttggc cttccaagat
    19441 agcgtggatg ctttgctgaa aagttatttc aattctgagc tagaaaatgg tggagccaag
    19501 tattctgaag gtgtctatgc agtcgccctt aacccaaaaa caggtgcggt tttgtctatg
    19561 tcagggatta aacatgactt gaaaacggga gagttgacgc ctgattcctt gggaacggta
    19621 accaatgtct ttgttccagg ttcggttgtc aaggcggcga ccatcagctc aggttgggaa
    19681 aatggagtct tgtcaggaaa ccagaccttg acagaccagt ccattgtctt ccaaggttca
    19741 gctcccatca attcttggta tactcaggct tacggttcat tccctatcac agcggtccaa
    19801 gctctggagt attcatctaa tacctatatg gtccaaacag ccttaggtct tatggggcaa
    19861 acctatcaac ccaatatgtt tgtcggcacc agcaatctag agtctgctat ggagaaactg
    19921 cgttcaacct ttggcgaata tggcttgggt actgcgacag gaattgacct accagatgaa
    19981 tctactggat ttgttcccaa agagtatagc tttgctaatt acattactaa tgcctttggg
    20041 cagtttgata actatacgcc gatgcagttg gctcagtatg tagcaactat tgcaaataat
    20101 ggtgttcgtg tggctcctcg tattgttgaa ggcatttatg gtaataatga taagggagga
    20161 ctgggtgact tgattcagca actgcaaccg acagagatga ataaggtcaa tatatccgac
    20221 tccgatatga gcatcttgca ccaaggtttt tatcaggttg cccatggtac tagtggattg
    20281 acaactggac gtgccttttc aaatggtgcc ttggtatcca ttagcggaaa aacaggtaca
    20341 gccgaaagct atgtggcaga tggtcagcaa gcaaccaata ccaatgcggt ggcctatgcc
    20401 ccatctgata atccccaaat cgctgtcgca gtggtctttc ctcataatac caatctaaca
    20461 aatggtgtag gaccttccat tgcgcgtgac attatcaatc tgtatcaaaa ataccatcca
    20521 atgaactaga aaggaaatta tgctttatcc aacacctatt gccaagttga ttgacagtta
    20581 ttctaagtta ccaggtatcg ggattaagac ggctacgcgt ctggcctttt atacgattgg
    20641 gatgtctgct gatgatgtca atgaatttgc aaaaaatctc ctttctgcta agagagaatt
    20701 gacatattgt tctatttgtg gacgtttgac agacgacgat ccttgttcta tctgtactga
    20761 tccgactcgt gaccagacaa caattttagt tcttgaggat agtagagatg tggcagccat
    20821 ggaaaatatc caagaatacc atggactcta tcatgtcctt catggcctca tttctcctat
    20881 gaatggtatc agtccggacg atatcaatct caagagcctt atgactcgtc ttatggatag
    20941 tgaggtttca gaagtgattg tggcgactaa tgctacagcg gatggtgaag cgacttccat
    21001 gtatctttca cgtttgctca agccggctgg tatcaaggtt acgcgtctag cacgaggtct
    21061 cgctgtggga gcggacattg agtatgcgga cgaagtgaca ctcttacgag ccattgaaaa
    21121 tcggacagag ttgtaagtgt aggcaaattt acgaactcca ttcatttata aaaaatcaaa
    21181 gaggctgaaa atcgttccta tcggcctctt tttgtatagt gtgatgagta ggctcaggtt
    21241 caagttttaa aaaaccaagc aaatatgata tactaaagag cgagtattct agtagaatta
    21301 ggacaaataa tatgaaacaa acgattattc ttttatatgg tggacggagt gcggaacgcg
    21361 aagtctctgt cctttcagct gagagtgtca tgcgtgcggt caattacgac cgtttcacag
    21421 tcaagacttt ctttatcagt cagtcaggtg actttatcaa aacgcaggaa tttagtcatg
    21481 ctccggggca agaagaccgt ctcatgacca atgaaaccat tgattgggat aagaaagttg
    21541 caccaagtgc tatctacgaa gaaggtgcag tggtctttcc agtccttcac gggccaatgg
    21601 gagaagatgg ctctgttcaa ggattcttgg aagttttgaa aatgccttac gttggttgca
    21661 acattttgtc atcaagtctt gccatggata aaatcacgac taagcgtgtt ctggaatctg
    21721 ctggtattgc ccaagttcct tatgtggcta tcgttgaagg cgatgatgtg actgctaaaa
    21781 tcgctgaagt ggaagaaaaa ttggtttatc cagtcttcac taagccgtca aacatggggt
    21841 ctagtgtcgg tatttctaag tctgaaaacc aagaagaact ccgtcaagcc ttaaaacttg
    21901 ccttccgata tgacagccgt gtcttggttg agcaaggagt gaatgcccgt gaaattgagg
    21961 ttggcctctt gggtaactac gatgtcaaga gcacgctacc aggagaagtt gtcaaggacg
    22021 ttgcctttta tgactacgat gccaagtata ttgataacaa gattactatg gatattcctg
    22081 ccaaaatcag tgatgatgtg gtggctgtca tgcgtcaaaa tgcagaaaca gccttccgtg
    22141 ccattggtgg ccttggtcta tctcgttgcg atttcttcta tacagataag ggagagattt
    22201 ttctcaacga gctcaatacc atgccaggtt tcacccagtg gtctatgtac ccactacttt
    22261 gggacaatat ggggatcagc tacccaaaac taatcgagcg tttggttgac cttgccaagg
    22321 aaagttttga caagcgcgaa gcgcatttga tataaaatga aagagagggt agaagccaga
    22381 accatcactg caaggtgact agagttctcg gacttcagcc ctttttaaag gagtagaaat
    22441 gaaattaaca atccatgaaa ttgcccaagt tgttggagtc aaaaatgata tcagtatctt
    22501 tgaggacacc cagttagaaa aagctgagtt tgatagtcgt ttgattggaa ctggagattt
    22561 atttgtgcca cttaaaggtg cgcgtgatgg ccatgacttt attgaaacag cctttgaaaa
    22621 tggtgcagca gtaaccttgt ctgagaaaga ggtctcaaat catccttaca ttctagtaga
    22681 tgatgttttg acagcctttc aatccctagc atcctactat cttgaaaaaa cgactgttga
    22741 tgtctttgct gttacaggtt caaatggcaa gacaacgact aaggatatgt tggcgcattt
    22801 actgtcaaca agatacaaga cctacaaaac acaaggcaat tacaataatg agattggcct
    22861 tccttacaca gttcttcata tgcctgaagg aacagaaaag ttggttttgg agatgggaca
    22921 ggatcacttg ggagatattc atctcttgtc tgaattggct cgtccaaaaa cagccatcgt
    22981 gaccttggtt ggagaagctc atttggcctt tttcaaagat cgttctgaga ttgctaaagg
    23041 aaaaatgcaa attgcagacg gaatggcttc aggttccttg cttttaatgc cggctgaccc
    23101 tatcgtagag gactatttgc caactgataa aaaggtggtc cgttttgggc aaggggcaga
    23161 gttggaaatc acagacttgg ttgagcgtaa ggacagtctg acctttaagg ctaatttctt
    23221 ggaacaagcc cttgatttgc cagtgactgg taagtacaat gctaccaatg ctatgattgc
    23281 atcctatgtt gccctacaag aaggagtttc agaggagcaa attcatcagg ccttccaagg
    23341 tcttgaattg acgcgtaatc gtactgagtg gaagaaagca gccaatggag cagatatcct
    23401 gtcagacgta tataatgcca atccaactgc tatgaagctg attttagaga ctttctctgc
    23461 cattccagcc aatgaaggtg gcaagaaaat tgcagtgttg gcggatatga aggagcttgg
    23521 tgaccagtct attcaactcc ataaccagat gattttgagc ctttctccag atgtgcttga
    23581 taccgttatt ttctatggag aagacattgc tgaattagcc caattggcca gtcaaatgtt
    23641 cccaattggc cacgtttact acttcaagaa aacagaagac caagaccaat ttgaagacct
    23701 agtcaagcag gtcaaagaaa gccttggggc tcatgaccaa atcctgctca aaggctctaa
    23761 ctctatgaat ctagccaagt tggtagaaag tttagaaaat gaagacaagt gattttgtca
    23821 agtatttgca aagaatgatt gccattacag atactggctt aacctttaca aaagatccgt
    23881 ttgaccgtga gcgctacgaa gacttgcgag accttttatc tgaaatgttg aatcaagcat
    23941 cagaccttga ttccgaagaa gtggcagaag tcttgaagcc aacttctgct tatgcgactc
    24001 cgttaatgga cgtccgtgct tggattgttg aggacgaaaa gatttgtctg gttaggggac
    24061 aaggagagga tagttgggct ttgccaggtg gctttggtga agtcggctat tctccaactg
    24121 aaaatattct caaggaaatt gaagaagaaa ccggttttaa agccaaagtt gaaagactgc
    24181 tagctgtttt tgataccaat cgtttccaac tacagagcaa acaatatgca aagtttgttt
    24241 ttgaatgtaa gcttctcgat ggacaattcc aagaaaatca agaaatcgct gaccttcaat
    24301 tttttgcaat tgaccaactg ccgaatttat ctgaaaaacg cattaccaag gagcaaatcg
    24361 agattctttg gcaggtttat caaggtcaga gggagcaata tcttgactaa gaagatgatt
    24421 atcgtatttc taaattcatt tttcttaagt agcatggtat aataaaatgc aggaaaattt
    24481 tgaatcatga ggaagactag atgaatttat gggatatttt ctttacgact caggcaaccg
    24541 agccgcccaa atttgacctt ttttggtatg ttagcctatt tacgctctta gccttaacct
    24601 tttatacagc ccatcgctat cgtgaaaaga aggtttacca acgatttttc caaatcttgc
    24661 agactgttca gttaatcctt ctttatggtt ggtactgggt caatcatatg ccactgtcag
    24721 aaagcctacc cttttaccat tgccgtatgg ctatgtttgt ggtactcttg cttcctggtc
    24781 agtccaaata taaacaatac tttgcattat tgggaacatt tgggacatta gcagcctttg
    24841 tttatccagt gccagatgct tacccttttc cacatatcac cattctatcc tttatctttg
    24901 gtcatttagc actcttgggg aactctctag tttatctatt gagacagtat aatgcgcgat
    24961 tgctggatgt gaagggaatt tttctcatga cctttgccct aaatgccttg atttttgtgg
    25021 tcaatttggt gacaggtggc gattacggat ttttgacaaa accgccattg gttggggatc
    25081 acggtctagt agctaattat ttacttgttt caattgtgct ggtagctact atcagtttga
    25141 ctaagaaaat cttagaattc tttttagctc aagaagcaga aaaaatgatt gcaaaggaag
    25201 cttaacacag agctttcttt tttgctctta gagagttttt acaagcagct tataaaataa
    25261 gaatttctga atagacaaac tcaaaaaatg gctgggaaat ttaggaaaaa agcaagcacg
    25321 attaaatttt ttgtgttata atattttgtg aatagctatg cctatgttta gctatggaat
    25381 aatacgaagt gcgaaacttg gaagatagag aggaagcgat gtaatggcta gagaaggctt
    25441 ttttacaggt ctagatattg gaacaagctc tgtcaaggtg cttgtggccg agcagagaaa
    25501 tggtgaatta aatgtaattg gcgtgagtaa tgccaaaagt aaaggtgtaa aggatggaat
    25561 tattgttgat attgatgcag cagcaactgc tatcaagtca gccatttccc aagcggaaga
    25621 aaaggcaggc atttcgatta aatcagtgaa tgtcggcttg cctggtaatc ttttgcaggt
    25681 agaaccaact caggggatga ttccagtaac atctgatact aaggaaatta cggatcaaga
    25741 tgttgaaaat gttgtcaaat cagctttgac aaagagtatg acacctgacc gtgaagtcat
    25801 tacctttatt cctgaagaat ttattgtgga tggtttccaa gggattcgtg acccacgtgg
    25861 catgatgggg gttcgccttg aaatgcgtgg tttgctttat acaggacctc gtactatctt
    25921 gcacaatttg cgtaagacgg ttgagcgtgc aggtgttcag gttgaaaatg ttatcatttc
    25981 accactagca atggttcagt ctgttttgaa cgaaggggaa cgtgaatttg gtgctacagt
    26041 gattgatatg ggggcaggtc aaacgactgt cgctacaatc cgtaatcaag aactccagtt
    26101 cacacatatt ctccaagaag gtggagatta tgtaactaaa gatatctcca aggttttgaa
    26161 aacctctcgc aaattagcgg aaggcttgaa actgaattac ggggaagcct atccgcctct
    26221 tgcaagcaaa gaaaccttcc aagtagaggt tattggagaa gtagaagcag tcgaagtgac
    26281 ggaagcctac ttgtcagaaa ttatttctgc acgaatcaag cacatccttg aacaaatcaa
    26341 gcaagaatta gatagaaggc gtctattgga cctccctggt ggtattgtct taatcggtgg
    26401 gaatgccatt ttaccaggta tggttgagct tgctcaggaa gtctttggcg tccgtgtcaa
    26461 gctttatgtt ccaaatcaag ttggtatccg taatccagcc tttgcgcatg tgattagttt
    26521 atcagaattt gcgggtcaat taacagaagt taatcttttg gctcagggag cgataaaagg
    26581 tgagaatgac ttaagtcatc agccaattag ttttggtggg atgctgcaaa aaacagctca
    26641 gtttgtacaa tcaacgcctg ttcaaccagc tcctgctcca gaagtagagc cggtggcgcc
    26701 tacagaacca atggcggatt tccaacaagc ttcacaaaat aaaccgaaat tagcagatcg
    26761 tttccgtgga ttgatcggaa gcatgtttga cgaataaaga ggaaaaataa attatgacat
    26821 tttcatttga tacagctgct gctcaagggg cagtgattaa agtaattggt gtcggtggag
    26881 gtggtggcaa tgccatcaac cgtatggtcg acgaaggtgt tacaggcgta gaatttatcg
    26941 cagcaaacac agatgtacaa gcattgagta gtacaaaagc tgagactgtt attcagttgg
    27001 gacctaaatt gactcgtggt ttgggtgcag gaggtcaacc tgaggttggt cgtaaagccg
    27061 ctgaagaaag cgaagaaaca ctgacggaag ctattagtgg tgccgatatg gtcttcatca
    27121 ctgctggtat gggaggaggc tctggaactg gagctgctcc tgttattgct cgtatcgcca
    27181 aagatttagg tgcgcttaca gttggtgttg taacacgtcc ctttggtttt gaaggaagta
    27241 agcgtggaca atttgctgta gaaggaatca atcaacttcg tgagcatgta gacactctat
    27301 tgattatctc aaacaacaat ttgcttgaaa ttgttgataa gaaaacaccg cttttggagg
    27361 ctcttagcga agcggataac gttcttcgtc aaggtgttca agggattacc gatttgatta
    27421 ccaatccagg attgattaac cttgactttg ccgatgtgaa aacggtaatg gcaaacaaag
    27481 ggaatgctct tatgggtatt ggtatcggta gtggagaaga acgtgtggta gaagcggcac
    27541 gtaaggcaat ctattcacca cttcttgaaa caactattga cggtgctgag gatgttatcg
    27601 tcaacgttac tggtggtctt gacttaacct tgattgaggc agaagaggct tcacaaattg
    27661 tgaaccaggc agcaggtcaa ggagtgaaca tctggctcgg tacttcaatt gatgaaagta
    27721 tgcgtgatga aattcgtgta acagttgttg caacgggtgt tcgtcaagac cgcgtagaaa
    27781 aggttgtggc tccacaagct agatctgcta ctaactaccg tgagacagtg aaaccagctc
    27841 attcacatgg ctttgatcgt cattttgata tggcagaaac agttgaattg ccaaaacaaa
    27901 atccacgtcg tttggaacca actcaggcat ctgcttttgg tgattgggat cttcgccgtg
    27961 aatcgattgt tcgtacaaca gattcagtcg tttctccagt cgagcgcttt gaagccccaa
    28021 tttcacaaga tgaagatgaa ttggatacac ctccattttt caaaaatcgt taagtaaatg
    28081 aatgtaaaag aaaatacaga acttgttttt cgagaagttg cagaggctag tctgagtgct
    28141 catcgagaga gtggttcggt ctctgtcatt gcagttacca agtatgtaga tgtaccgaca
    28201 gcggaagcct tgcttccgct aggtgttcat catatcggtg aaaatcgtgt agataagttt
    28261 ctggaaaaat atgaagcttt aaaagatcga gatgtgactt ggcatttgat tggtaccttg
    28321 caaagacgta aggtgaaaga tatcattcaa tacgttgatt atttccatgc attggactca
    28381 gtaaagctag caggggaaat tcaaaaaaga agtgaccgag tcatcaagtg tttccttcaa
    28441 gtaaatattt ctaaagaaga aagcaaacac ggtttttcga gagaggaact gctggaaatc
    28501 ttgccagagt tagccagact agataagatt gaatatgttg gtttaatgac gatggcacct
    28561 tttgaggcta gcagtgagca gttgaaagag attttcaagg cggcccaaga tttacaaaga
    28621 gaaattcaag agaaacaaat tccaaatatg cctatgactg agttaagtat gggaatgagt
    28681 cgtgattata aagaagcgat tcaattcggt tccacttttg ttcgtatagg tacatcattt
    28741 tttaagtagg agagaaccat gtctttaaaa gatagattcg atagatttat agattatttt
    28801 acggaggatg aggattcaag tctcccttat gaaaaaagag atgagcctgt gtttacttca
    28861 gtaaattctt cacaggaacc ggctctccca atgaatcaac cttcacagtc ggctgggaca
    28921 aaagagaaca atattaccag acttcatgca agacaacagg aattggcaaa tcagagtcag
    28981 cgtgcaacgg ataaggtcat tatagatgtt cgttatccta gaaaatatga ggatgcaaca
    29041 gaaattgttg atttattggc aggaaacgaa agtatcttga ttgattttca gtatatgaca
    29101 gaggtgcagg ctcgtcgttg tttggactat ttggatggag cttgtcatgt tttagctgga
    29161 aatttgaaaa aggtagcttc taccatgtat ttgttgacac cagtgaatgt tattgtaaat
    29221 gttgaagata tccgtttacc agatgaagat caacagggtg agttcggttt tgatatgaag
    29281 cgaaatagag tacgataatg atttttttaa ttcgtatgat ttataatgca gtggatattt
    29341 actccctgat tttggtagcc ttcgctgtca tgtcttggtt tccaggtgcc tacgaatcca
    29401 gtttaggtcg ttggattgta gcgttggtga aaccagtgct tgctcccttg caacgcctgc
    29461 ctttacagat agcgggtctt gatttatctg tttgggttgc gattgttttg gttcgatttt
    29521 taggagaaaa cctagtgcgt tttctggcga tgataggatg aataaaggga tttatcagca
    29581 tttctccata gaagatcgtc catttcttga caagggaatg gaatggataa agaaggtaga
    29641 agatagctat gctccttttt taactccttt tatcaatcct catcaggaga agctattaaa
    29701 gattttggcc aaaacctatg gtcttgcttg tagcagtagt ggggaattcg tatcgagtga
    29761 gtatgttcga gttttattat acccagatta tttccaacca gagttttcag attttgaaat
    29821 atctctccag gaaattgtgt attccaataa atttgaatat ttaacgcatg ctaagatttt
    29881 agggacagtc atcaatcaat tagggattga acggaaactt tttggagata tcctagtaga
    29941 tgaagaacgg gcgcagatta tgattaatca gcagtttctt cttctctttc aagatggact
    30001 aaagaaaatt ggtcgtatac ctgtttcgct ggaggaacgt cctttcaccg agaaaataga
    30061 taagctagaa cagtatcgag aactggattt atctgtgtct agttttcgat tagatgttct
    30121 tttatcaaat gttttgaaac tatctaggaa tcaagcaaac cagttgattg aaaagaaact
    30181 tgtccaagta aattatcatg tggtagacaa atcagattac actgttcaag ttggagactt
    30241 gattagtgtg agaaaatttg gtcgcttgag attacttcaa gataagggac aaacgaaaaa
    30301 agagaagaaa aaaataaccg tccagttatt attaagtaag tgaggaatag aatgccaatt
    30361 acatcattag aaataaagga caagactttt ggaactcgat tcagaggttt tgatccagaa
    30421 gaagtcgatg aatttttaga tattgtggtt cgtgattacg aagatcttgt gcgtgcgaat
    30481 catgataaaa atttgcgtat taagagttta gaagagcgtt tgtcttactt tgatgaaata
    30541 aaagattcat tgagccagtc tgtattgatt gctcaggata cagctgagag agtgaaacag
    30601 gcggcgcatg aacgttcaaa caatatcatt catcaagcag agcaagatgc gcaacgcttg
    30661 ttggaagaag ctaaatataa ggcaaacgag attcttcgtc aagcaactga taatgctaag
    30721 aaagtcgctg ttgaaacaga agaattgaag aacaagagcc gtgtcttcca ccaacgtctc
    30781 aaatctacaa ttgagagtca gttggctatt gttgaatctt cagattggga agatattctc
    30841 cgtccaacag ctacttatct tcaaaccagt gatgaagcct ttaaagaagt ggttagcgaa
    30901 gtacttggag aaccgattcc agctccaatt gaagaagaac caattgatat gacacgtcag
    30961 ttctctcaag cagaaatgga agaattacaa gctcgtattg aggtagccga taaagaattg
    31021 tctgaatttg aagctcagat taaacaggaa gtggaaactc caactcctgt agtgagtcct
    31081 caagttgaag aagagcctct gctcatccag ttggcccaat gtatgaagaa ccagaagtag
    31141 ctccagtgca tccgacaggt ccaacaccag ctacagaaac tgttgattca gcaccgggat
    31201 ttgaagcacc gcaagaatct gttacaattt tataagaaat attctgagaa caatatctta
    31261 tccttatatt cccagcgagc aggagatggt gtgagtcctg cattccctat cgataagatt
    31321 atcctctcaa aaactcaagt ctgaatctag taagatttga cgttccccac gttacgggat
    31381 aagagggaga aagactaaaa ctttttccga ataaaggtgg taccacgatt ttcgtccttt
    31441 ttggaagtcg tggtttttaa tttgttatta tttataaagg agataccatg aaactcaaag
    31501 acacccttaa tcttgggaaa actgaattcc caatgcgtgc aggccttcct accaaagagc
    31561 cagtttggca aaaggaatgg gaagatgcaa aactttatca acgtcgtcaa gaattgaacc
    31621 aaggaaaacc tcatttcacc ttgcatgatg gccctccata cgctaacgga aatatccacg
    31681 ttggacatgc tatgaacaag atttcaaaag atatcattgt tcgttctaag tctatgtcag
    31741 gattttacgc accatttatt cctggttggg atactcatgg tctgccaatc gagcaagtct
    31801 tgtcaaaaca aggtgtcaaa cgtaaagaaa tggacttggt tgagtacttg aaactttgcc
    31861 gtgagtacgc tctttctcaa gtagataaac aacgtgaaga ttttaaacgt ttgggtgttt
    31921 ctggtgactg ggaaaatcca tatgtgacct tgactcctga ctatgaagca gctcaaattc
    31981 gtgtatttgg tgagatggct aataagggtt atatctaccg tggtgctaag ccagtttact
    32041 ggtcatggtc atctgagtca gcacttgctg aagcagagat tgaataccat gacttggttt
    32101 caacttccct ttactatgcc aacaaggtaa aagatggcaa aggagttcta gatacagata
    32161 cttatatcgt tgtctggaca acgactccat ttaccatcac agcttctcgt ggtttgacgg
    32221 ttggtgcaga tattgattac gttttggttc aacctgctgg tgaagctcgt aagtttgtcg
    32281 ttgctgctga attattgact agcttgtctg agaaatttgg ctgggctgat gttcaagttt
    32341 tggaaactta ccgtggccaa gaactcaacc atatcgtaac agaacaccca tgggatacag
    32401 ctgtagaaga gttggtaatt cttggtgacc acgttacgac tgactctggt acaggtattg
    32461 tccatacagc ccctggtttt ggtgaggacg attacaatgt tggtattgct aataatcttg
    32521 aagtcgcagt gactgttgat gaacgtggta tcatgatgaa gaatgctggt cctgaatttg
    32581 aaggtcaatt ctatgaaaag gtagttccaa ctgttattga aaaacttggt aacctccttc
    32641 ttgcccaaga agaaatctct cactcatatc catttgactg gcgtactaag aaaccaatca
    32701 tctggcgtgc agttccacaa tggtttgcct cagtttctaa attccgtcaa gaaatcttgg
    32761 acgaaattga aaaagtgaaa ttccactcag aatggggtaa agtccgtctt tacaatatga
    32821 tccgtgaccg tggtgactgg gttatctctc gtcaacgtgc ttggggtgtt ccacttccta
    32881 tcttctacgc tgaagatggt acagctatca tggtagctga aactattgaa cacgtagctc
    32941 aactttttga agaacatggt tcaagcattt ggtgggaacg tgatgccaaa gacctcttgc
    33001 cagaaggatt tactcatcca ggttcaccaa acggcgagtt caaaaaagaa actgatatca
    33061 tggacgtttg gtttgactca ggttcatcat ggaatggagt ggtggtaaac cgtcctgaat
    33121 tgatttaccc agccgacctt tacctagaag gttctgacca ataccgtggt tggtttaact
    33181 catcacttat cacatctgtt gccaaccatg gcgtagcacc ttacaaacaa atcttgtcac
    33241 aaggttttgc ccttgatggt aaaggtgaga agatgtctaa atctcttgga aataccattg
    33301 ctccaagcga tgttgaaaaa caattcggtg ctgaaatctt gcgtctctgg gtaacaagtg
    33361 ttgactcaag caatgacgtg cgtatctcta tggatatttt gagccaagtt tctgaaactt
    33421 accgtaagat tcgtaacact cttcgtttct tgattgccaa tacatctgac tttaacccag
    33481 ctcaagatac agtcgcttac gatgagcttc gttcagttga taagtacatg acgattcgct
    33541 ttaaccagct tgtcaagacc attcgtgatg cctatgcaga ctttgaattc ttgacgatct
    33601 acaaggcctt ggtgaacttt atcaacgttg acttgtcagc cttctacctt gattttgcca
    33661 aagatgttgt ttacattgaa ggtgccaaat cactggaacg ccgtcaaatg cagactgtct
    33721 tctatgacat tcttgtcaaa atcaccaaac tcttgacacc aatccttcct cacactgcgg
    33781 aagaaatttg gtcatatctt gagtttgaaa cagaagactt cgtccaattg tcagagttac
    33841 cagaggctca aacttttgct aatcaagaag aaatcttgga tacatgggca gccttcatgg
    33901 acttccgtgg acaagcacaa aaagccttgg aagaagctcg taatgcaaaa gttatcggta
    33961 aatcacttga agcacacttg acagtttatc caaatgaagt tgtgaaaact ctactcgaag
    34021 cagtaaacag caatgtagct caacttttga tcgtgtctga gttgaccatc gtagaaggac
    34081 cagctccgga agctgccctt agcttcgaag atgtagcctt cacagttgaa cgtgctactg
    34141 gtgaagtatg tgaccgttgc cgtcgtatcg acccaacaac agcagaacgc agctaccagg
    34201 cagttatctg tgaccactgt gcaagcatcg tagaagaaaa ctttgcggaa gcagtcgcag
    34261 aaggatttga agagaaataa gattgaaaag tctaggcaaa attcaatttg agaagaaaag
    34321 acaactaatt ttatagtcta ttaaacgcat tgtatcacgt ttttgaatac ctgatatgat
    34381 gcgtttttta tttattttaa aaatttgcga ggtatgactt tttatactca acaagaatca
    34441 aagagaaact tagcaatcta acagtagtaa gataaaatag gaatttgata ttagggataa
    34501 gattggtaaa tagtgtaata tttttacaac aataaattta tatagttatt tctggtttct
    34561 gaaaagtatt atattttatt tcatattata caaattttta ttttataata tcagaacata
    34621 ctttttttaa aagcaaatat gatacaattt tatttgaaaa aaataaaaaa ggagatttta
    34681 ttataaaatt aaaaagactt gctttaatta gtggtatcgt cggtcttgtg ggaggaattt
    34741 tacttcttat tggtcctttt gtcttgttgg gaacagcggt aaacacagct gctacaactc
    34801 ttaatggagg agctactgca ggggcttttt caggtgtagc cttactcttg aatgccttga
    34861 agattgcaaa tcttgttctt ggtatcattg ctattgttta ctataaagga gataagcgtg
    34921 taggtgcagc tccgtctgta ctaatgattg tttctggtgg agttagtctc attccattct
    34981 taggatgggt tggggggatt cttgctatta tcggaggatc tctattcctt gcaacattga
    35041 agaaattcaa atcagaagaa taaaaggtat tttagcatga aaagaacaaa aagtttatcg
    35101 gtataggagt agctctatta tctctttctc ttctagttgc atgtggaaca taaagttcaa
    35161 agaatacttc aacaagtaat gatgagaaga cagtagcaac atccaatagt tcaaaagaaa
    35221 caatcacttt cgatacaccg gttgtaacag acgatgcgat tgaatcaata cgcacttatg
    35281 cagattatat agatctttat aaaaatattt ttgatgatta ttttactaaa gctgaggaag
    35341 gtttcaaagg cacagctatg gaaaataatg actcgtttac taaactaaaa gagtcaactc
    35401 aaaaattatt cgatgcgcag aaaaaaaggt taaataatga agatagaata gaaacaacca
    35461 aaaacaatgt gattgccaaa cattgtcaaa cagtcctttc ctttttggtt ttgactagct
    35521 tttttgtgaa aaattgtgta aaatagaata gataaacgag gggaaacctc ggaaaattta
    35581 aaggagaatc catctaatgg taaaattggt ttttgctcgc cacggtgagt ctgaatggaa
    35641 caaagctaac cttttcactg gttgggctga tgttgatttg tctgaaaaag gtacacaaca
    35701 agcgattgac gctggtaaat tgatcaaaga agctggtatc aaatttgacc aagcttacac
    35761 ttcagtattg aaacgtgcta tcaaaacaac taacttggct cttgaagctt ctgaccaatt
    35821 gtgggttcca gttgaaaaat catggcgctt gaacgaacgt cactatggtg gtttgactgg
    35881 taaaaataaa gctgaagctg ctgaacaatt tggtgatgag caagttcaca tctggcgtcg
    35941 ttcatacgat gtattgcctc caaacatgga ccgtgatgat gagcactcag ctcacacaga
    36001 ccgtcgttac gcttcacttg acgactcagt tatcccagat gctgaaaact tgaaagtgac
    36061 tttggaacgt gctcttccat tctgggaaga taaaatcgct ccagctctta aagatggtaa
    36121 aaacgtattc gtaggagctc acggtaactc aatccgtgcc cttgtaaaac acatcaaagg
    36181 tttgtcagat gacgagatca tggacgtgga aatccctaac ttcccaccat tggtattcga
    36241 attcgacgaa aaattgaacg tcgtttctga atactacctt ggaaaataaa aaattgtaag
    36301 tctagaattg atttctaggc tttttatgtt agtatggaag tatgataagg aataaaaaac
    36361 aagattatgt actggcctac aagcaaccag cttcaaccac ttacatgggt tgggaagaag
    36421 aagctttacc gataggcaat ggttctttag gagcaaaagt atttggcctt ataggggctg
    36481 aacggattca atttaatgaa aaaagtctct ggtctggagg tccacttcct gatagttcag
    36541 attatcaggg tggaaatctt caggatcagt atggtttttt agctgagatt cggcaggctt
    36601 tggagaagag agattacaat ctggctaagg aactggctga gcagcaccta attgggccaa
    36661 aaacgagtca atatgggacc tatctgtctt ttggggatat tcacattgag ttcagccagc
    36721 aaggtacgac tttgtctcag gtgacggact atcagagaca gctgaatatt agtaaggcac
    36781 ttgcgacgac ttcttatgtc tataagggaa cgcgatttga acgtaaagct tttgcgagtt
    36841 ttccagatga tctcttgatt caatgtttta ctaaggaagg gttggaaact ctagatttta
    36901 ctatagaact atccttgacc tgtgatttgg cttctgatgg aaagtatgag caggaaaaat
    36961 ctgattacaa ggagtgtaag ttggatatta ctgattctca tatcttgatg aagggaagag
    37021 ttaaggataa tgatctgcgg tttgctagtt atctagcttg ggaaacggat ggagatatta
    37081 gagtttggtc agatagggtt cagatatcag gagccagtta tgccaatctc ttcttggccg
    37141 ctaagacgga ttttgcccaa aatcctgcta gcaattatcg caagaaacta gatttagagc
    37201 aacaggtgat agacttggtg gacacagcta aagaaaaggg ctatacccaa ttgaaatcaa
    37261 ggcatatcga ggactaccaa gccttattcc agcgtgttca attggatttg gaagctgatg
    37321 ttgacgcatc cactacagat gatttgttaa aaaattataa gccacaagaa gggcaggctt
    37381 tggaggagct gttcttccag tatggacggt atttattgat tagttcgtcc agagactgcc
    37441 cagatgctct accagctaac ctacagggag tctggaatgc ggtcgacaat cctccttgga
    37501 attcggacta tcacttaaat gtcaatctgc agatgaatta ttggccagcc tatgttacca
    37561 atctcctaga gacggtcttt ccagtcatca actatgtaga tgatttgcgt gtctatggtc
    37621 gtctagcggc tgtaaagtat gcaggaatcg tctctcagaa aggtgaggag aatggttggt
    37681 tggttcatac tcaagcgact ccctttggtt ggacggcacc tggttgggat tactattggg
    37741 gttggtcacc agctgccaat gcgtggatga tgcaaaccgt ttatgaagcc tattcatttt
    37801 atagggacca agactatctc agggagaaaa tttatcccat gttgagggaa acggttcgtt
    37861 tttggaatgc ctttttacat aaggatcagc aggcgcagcg ttgggtgtct tctccgtctt
    37921 attccccaga acatgggccg atttcgattg gcaataccta tgaccaatct ctgatttggc
    37981 agttatttca tgattttatt caggctgctc aggaattggg actggatgag gacttgttga
    38041 ctgaggttaa ggagaagtct gatttactaa atcctttgca aatcactcaa tctggtcgaa
    38101 tcagggagtg gtatgaggag gaagagcagt attttcaaaa tgagaaagtg gaggcccagc
    38161 atcggcacgc ttcccatcta gtgggactct atcctggcaa tctctttagc tacaagggac
    38221 aagagtatat tgaagcggcg cgtgctagcc tcaatgatcg tggagatggc ggcacaggct
    38281 ggtccgaggc taataagatc aatctctggg cgcgtttggg agatggcaat cgagcccata
    38341 aattattggc agagcagtta aagacatcca ccttgcaaaa tctttggtgt agccatcctc
    38401 cttttcagat agatggtaat tttggtgcta ctagtggcat ggcagaaatg ttactccagt
    38461 ctcatgcagc ttatctggta cctctagctg ccctacctga tgcttggtca acaggttctg
    38521 tttcaggctt aatggcacgt ggacattttg aagtgagcat gagctgggaa gataaaaaac
    38581 tcttacagtt gaccatttta tcaaggagtg gaggagattt gcgagtttct tatccagata
    38641 ttgagaagag tgtgattaaa atgaatcaag aaaaaataaa agcgaaatgc atggggaaag
    38701 attgtatttc ggtggcaaca gcagaaggtg atcttgttca attttatttt taagaagatg
    38761 ttataaggca gtaatttgaa actgcctttt aataaggatt taagaatata ggcagtttcc
    38821 aactagttga aaaagcgtta taatgataat aggaagtaat actcaatgaa aatcaaagag
    38881 cacaaacaag gaagctagcc gcaggttgct caaaacagtg ttttgaggtt gcagatggaa
    38941 gctgacgtgg tttgaagaga gattttcgag gagtataatt tgtttgatag agggtgggtc
    39001 tgatggctta tattgagatg aaacactgtt acaagcgtta tcaggttggg gacacggaga
    39061 ttgtggccaa tcgtgatgtg aattttgaga ttgaaaaggg ggaactggtt attatccttg
    39121 gtgcttcagg tgcaggcaag tcaacagttc ttaaccttct tgggggaatg gataccaatg
    39181 atgaagggga aatctggatt gatggtgtta atattgcgga ttatagttcc caccagcgca
    39241 ccaattaccg tagaaatgat gtggggtttg tttttcagtt ttataatcta gtttctaatc
    39301 tgacagctaa ggaaaatgtg gaactggctt ctgaaattgt gacagatgcc ttgaattctg
    39361 atcaggtctt gacagatgta ggtctggctc atcgtctaaa taactttcca gcccagcttt
    39421 ctggagggga gcaacagcga gtctccattg cacgcgcggt agccaaaaat cctaaaattc
    39481 ttctttgtga tgaacctact ggagccttgg attatcagac gggcaagcag gttttgaaaa
    39541 ttctccaaga catgtctcgt caaaagggag cgacggtgat catcgtgact cataatggag
    39601 ctttggcgcc cattgctgat cgcgtgattc atatgcacga tgccagtgtc aaggatgtgg
    39661 tgctcaacca gcatcctcag gatattgaca gtttggagta ctagtatgat caagcgaaaa
    39721 acttattgga aggacttaat tcagtccttc acaggctcca aggggcgttt tttatccatc
    39781 ttgatcctga tgatgttggg atctctagcc ttagtaggcc tcaaagtaac cagtcccaac
    39841 atggaggcga cagctaatgc ttatttaaca actgctcaaa ccttggattt ggcagtcatg
    39901 tctaactatg gcttggatca agcagaccaa gaagaactaa aacagacgga gggcgcagag
    39961 gtcgagtttg gctatttgac agatgtgact atggataatg ggcaggatgc cattcgactg
    40021 tactccaaac cagagcgaat ttcaaccttt cagctaagaa agggacgact tcctcagtca
    40081 gacaaggaaa tcgctttggc cactcatttg caaggccaat acagcgtggg acaggagatt
    40141 agttttaaag aaaaagaaga gggtcattcc tctttaaaag accatactta taccattact
    40201 ggttttgtgg attcggctga aatcctctcc cagcgagata tgggctacgc aggaagtgga
    40261 agtgggactc tgacagccta tggggtgatt ttacctagtc agtttgatca gaaagtctac
    40321 aatatagctc gtttgaaata tcaagattta gcaggtttaa atgccttttc atcagcttat
    40381 gaagaaaaat ccaagcaaca tcaggaagac cttgaacaaa ctttatcaga taatggcaag
    40441 gtacgtctgc aacttttgaa aaaagaagga caagagtctc tagacaaggg gcaagagacc
    40501 cttgacaagg ctcagactaa tttgcaggaa ggcaagcgtc gtttagcagc tgctcaagct
    40561 cgtatacagg ctcaagaaag tcaactagcc ttgtttcctc aagttcagag agagcaggct
    40621 agtgctcaac ttacccaagc caagcaggaa ttgggcaagg aagaggataa actaaagcaa
    40681 gctgaacaaa atctagccca agaaaaggaa aaattagaaa aacatcagca agtcttggac
    40741 gatttggcgg agccaaggta tcaggtctat aatcgtcaga ccatgccagg tggtcagggc
    40801 tatcttatgt atagcaatgc ttcatccagt attcgagcag tgggcaatat ctttcctgtg
    40861 gtactttatg ccgtagcagc catggtgacc tttacgacca tgactcgctt tgtagacgaa
    40921 gagcgaactc atgcagggat ttttaaggcc ttgggttatc gtagtaagga tattatcgcc
    40981 aagtttctcc tttatggact agtagctggg actgtcggaa cggctctagg tagtatactt
    41041 ggtcattatt tgctagccag tgtaatttca agtgtcatta caaaaggcat ggtggtggga
    41101 gaaactcaga ttcagttcta ttggacctat agcttactag cttttgtctt gagcttgttg
    41161 gcgagtgtgt taccagccta tctggtggct tggagggaac ttcatgacga agcagcccag
    41221 cttctacttc ctaaacctcc tgtcaaagga gctaaaatct tattggagcg tatcggtttt
    41281 atctggcgtc gtctcagttt tactcataag gtaacagccc gcaacatctt tcgttataag
    41341 cagagaatgt tgatgacaat ctttggtgtg gcaggttctg tagctctgct ctttgcaggt
    41401 ttgggaatcc aatcttctgt agcaggagtt ccgtctaaac agtttcaaca aatccaacag
    41461 tatcagatgc ttgtctctga aaatcctagt gcgaccaatc aggacaaggt agagctagca
    41521 gaagtgttga aagggcagga gatactagcc taccagaaaa tctattctaa agcgctatac
    41581 aaggatttca aaggcaaagc tggtcttcaa aacattactc ttatgatgat agagaaggaa
    41641 gatttgactc cctttatcca tcttcaacat catcagcagg agctgacatt aaaagatggc
    41701 atcgttatta cagctaaact cgcccagctg gcaggtgtca aggttgggca gactttagaa
    41761 attgaaggta aggaactaaa ggtcgctgct attactgaga actacgttgg tcactttatt
    41821 tatatgagtc aggctagcta tgagcaactt tacggacagc taccccaagc caacacttat
    41881 ctggtctcat taagggatac cagtgcaact agtatcgaaa gtcaggcggg cttgcttatg
    41941 aatcaatctg cggtgtccag cgttgtccaa aatgcttcag ccattcgact cttcgactct
    42001 atcgctagct cactcaatca gaccatgacc atcttggtca tcgtatcggt tctattagct
    42061 attgtcatcc tttacaatct gaccaatatc aacgtagctg agagaatccg tgaactctcc
    42121 actatcaagg ttcttggttt tcataataat gaagtcaccc tctacattta ccgtgagacg
    42181 attgtgctgt cccttgtggg aatcgtactt ggtctgatag ctggtttcta tttacaccaa
    42241 tttttgattc aaatgatttc gcctgcgact attctctttt atccgcaggt aggctgggaa
    42301 gtctatgtaa tcccagtggc agcagtaagc atcattttga ccttgcttgg tttcttcgtc
    42361 aattattatc tgagaaaggt tgatatgtta gaagccctga aatctgtaga gtaaggtagt
    42421 tatttttagc tgattgaact tctatttact aatattcaaa aatcctccgt ttcaaagagc
    42481 agggaactct ttgtgacaga ggattttttc tatagggctt tagcagctgc aattgcggct
    42541 tcgaagtttg gctcagaatt gatattatcc acgtattcaa cgtagcgaat cgtattgtca
    42601 gtatcgagga caaagactgc gcgtgctaat aggtgccatt cgttgatcaa gagggcataa
    42661 tcgcgcccga aagaatggtc aaagtagtct gaaagcataa tggcattgtc aaggccttca
    42721 gcaccgcacc aacgtttttg agcaaaaggt aggtccattg aaacagtcaa tacgaccgtg
    42781 ttgtccagtc cagccaattc ttcattaaaa cgacgtgttt gagttgagca gatgcctgta
    42841 tcgatagaag gaacgacact caagactttt ttcttgccat caaaatcagc cagagatttt
    42901 ttagaaagat ctgttgtagt aagagaaaaa tcaagcgcct tgtcgccgac ttgtagttgt
    42961 ttacctgtaa agctcacagg atttccgaga aaagttacca taggatactc caatcttttt
    43021 tcttccattg tatctgaaac agtcagaatt ttccaatgat ttgaccggaa atgtgggcat
    43081 agaaaaaacg ccagctcaca tgagaatgac gtttttcaga ggcttatttt gccaatcctt
    43141 cagcaatctt gtcaaggttg tatttcatca tgctgtagta gctgtcgcct tctttacctt
    43201 gttctgcgat agagtcagta aagatttgtg cgtagattgg gatgtttgtg tcttgagaaa
    43261 cagttttcat tggacggtca tccacacttg attctacaaa gagtgatgga acttttgttt
    43321 ggcgaagttt ttcaaccaag gtcttgattt gttcaggagt tccttcttct tcagtattga
    43381 tttcccagat gtaggcactt gggacaccat aggctttaga gaagtatttg aatgctcctt
    43441 cgctggttac aatgagtttc ttttcagcag ggatcttatt aaatttatcc ttactttctt
    43501 tatcaagttt gtctaactta tcagtatatt ctttgagatt tttttcatag aattctttat
    43561 tgttagggtc tttggcgctc aattgtttgg cgatattttt agcaaaaata ataccgtttt
    43621 caaggttaag ccaagcgtgt gggtcttctt ttcctttttc attttgacct tcaaggtaga
    43681 taacatcaac gccgtcgctg actgcgaagt agtctttgtt ttcagttttc ttggcatttt
    43741 ctaccaattt tgtaaaccaa gcattgccac ctgtttcaag gttgataccg ttatagaaaa
    43801 tcaaatcagc ctcagaagtt ttcttaacgt cttcaggaag tggttcgtat tcgtgtgggt
    43861 cttgcccaat cggaacgata ctatgaaggt caattttgtc accagcaata tttttagtaa
    43921 tatcagcgat gattgagttt gtagcaacaa cttttagttt ttgaccagaa gttgtatctt
    43981 tttttccgct agcacatgct acaagaatga ttgcagaaag aaagagaacg agtaatgtac
    44041 ctaatttttt cattagatcc tccaatttat tagggctttg ccccttattt taacaaatgt
    44101 ttatttttca gtttcaaata tcgttgtttg ggagcgataa agaagctaat gagaaagaaa
    44161 ctagcagctg taagcacgat actagaacct gccgcaacat taaaactata gccaataaag
    44221 agtcccaaaa ctgaagcagt agctccgaag gttgaggaaa ggaaaatcat gcttttcaga
    44281 ctattagcat acagataagc agttgcagct ggggtaatca gcatggctac aatcaggata
    44341 gttccgacac tttgcatggc tgtcacagac acgagagtca ggagtaccat gagaaggtag
    44401 tgatagaaat tgacaggcat tcccatggct ttagccaaga gttcatcaaa ggaagttatc
    44461 aagagttgct tgaagaaaat ccaaattaac aagaggatgg ctgcccccac acccatagta
    44521 ataaacatat ccgtatcttg gacggccaga atattaccaa aaaggatatg gaaaaggtca
    44581 gttgaacttt tagcgacacc aatcaagatg ataccgaggg ctaagaaaga agaaaaggta
    44641 atgccgatgg cggtatcgct tttgataatc gagtttcctt tgatgtaggt aatgatgatg
    44701 gcagctagca atccaaagac aatggctccg ataaagaagt caaggcccaa gatgaaggag
    44761 agggctacac ctggtaagac agcatgtgaa atggcatctc ccatgagtga catcccgcgt
    44821 agaataatga aacatcccac agctccagct acaatcccga cgacaatagc tgttatcaag
    44881 gcattttgta ggaaatggaa tttttgcaat ccatcgataa attctgcaat cataggtcac
    44941 ctccattgaa aaagagttga ttaccgtaag cttcttttag attggtttcg gtaaaagttt
    45001 cttttgttgg accaaaggca atcacttctc gattgacaag taagacttga tcgaagtagt
    45061 ggggaatctt gctgaggtcg tggtgaacga tgagaaccgt cttcccagct tttttcaaat
    45121 ctctcagcgt attcatgatg atttcctcac tgacagagtc aatcccagca aagggttcat
    45181 ccaagaggat atagtcggct tcctgcacca aacatctggc aatcaagacc cgctggaatt
    45241 gacctccaga cagttgacta atttgacgtt cagcgtagtc agctaggccg acgatttcaa
    45301 gggcctcttg cactttcttc caatgtttag cctttaaact tcgaaagaga ggaatagagg
    45361 gaaatagtcc taacgagacg cattccttga ccttgatggg aaagttgtag tcgatattga
    45421 ttttttgttc gacataggca attcggtgta aggatttttt aacttccttg tcatcgagaa
    45481 atgcctgacc ttgatgtggg ataattccca gcataccttt taatagtgtt gatttcccag
    45541 cgccgtttgg accaatgatg ccggtaattg ttggtccatg gagcactagt gaaatatcct
    45601 taagtgccaa cgtttctttg taggagacac tgaggttttc gatacgtatc ataaacttgt
    45661 at
//
LOCUS       DAVKWZ010000011        39257 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000011 DAVKWZ010000000
VERSION     DAVKWZ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39257
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.011"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(98..2356)
                     /locus_tag="VW854_000656"
     CDS             complement(98..2356)
                     /locus_tag="VW854_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV0261937.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDTKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(2585..2815)
                     /locus_tag="VW854_000657"
     CDS             complement(2585..2815)
                     /locus_tag="VW854_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEV0261938.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            3005..3688
                     /locus_tag="VW854_000658"
     CDS             3005..3688
                     /locus_tag="VW854_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV0261939.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSEIVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            3688..4422
                     /locus_tag="VW854_000659"
     CDS             3688..4422
                     /locus_tag="VW854_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV0261940.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKVIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            4576..5433
                     /locus_tag="VW854_000660"
     CDS             4576..5433
                     /locus_tag="VW854_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEV0261941.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAVGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLTIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            5575..6447
                     /locus_tag="VW854_000661"
     CDS             5575..6447
                     /locus_tag="VW854_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEV0261942.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEPMAFSLQDQQLLKEQLEKAEVILLGPGL
                     GDNAFGEDLVKQVFAGLKQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERVSKDKLV"
     gene            6729..7412
                     /gene="rpiA"
                     /locus_tag="VW854_000662"
     CDS             6729..7412
                     /gene="rpiA"
                     /locus_tag="VW854_000662"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEV0261943.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            7426..8637
                     /locus_tag="VW854_000663"
     CDS             7426..8637
                     /locus_tag="VW854_000663"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEV0261944.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            8639..9181
                     /locus_tag="VW854_000664"
     CDS             8639..9181
                     /locus_tag="VW854_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEV0261945.1"
                     /translation="MTRYALLVRGINVGGKNKVVMAELRQELTNLGLEKFESYINSGN
                     IFFTSIDSKAQLVEKLETFFAVHYPFIQSFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVESLELKDEVLYFGKLGIFWGKFSEESYSKTAYHKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            9197..10006
                     /locus_tag="VW854_000665"
     CDS             9197..10006
                     /locus_tag="VW854_000665"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV0261946.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            10503..11525
                     /locus_tag="VW854_000666"
     CDS             10503..11525
                     /locus_tag="VW854_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HEV0261947.1"
                     /translation="MKESENDAPKLETPLREEPRLAPQTLPEASEVLENKREESKVEI
                     TEPAQDSPILAPVEETKEEAVTEKPTNTRSLTAEDLVKISKGELHLENDLIDESFYGE
                     KALDLEGDDDQDGIKNKDGKDYLGYNSHPLLADSDGDGLADGEDDNKKEWYVTDRDSL
                     LFMELAYRDDDYIEKILYHKNLFPSLYLDRQEHKLMHNELAPFWKMKKAYYTDSGLDA
                     FLFETKSDLPYLKDGTVYMLAIRGTRVNDAKDLSADLVLLGGNKPAQADDIRKVVGEL
                     AKDISITKLYMTGHSLGGYLAQIAAVEDYQKYPDFYNHVLRKVTTFSAPKVITSRTVW
                     DAKNGF"
     gene            11535..12110
                     /locus_tag="VW854_000667"
     CDS             11535..12110
                     /locus_tag="VW854_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEV0261948.1"
                     /translation="MESRKLAVSGKIKHYVVDNDNVVTPLIHNNRDIVTFTGNSRFKH
                     RSRGYFESPMNDIPNFNIGKQATLDKHGYRDPKLDKVRFFKKQALPRSFSQPSAEPME
                     NIALGKQVTQSSTVFGGDARRAVDGKVDGNYGHNSVTHTNFQSKPWWQVDLAKEETIR
                     QINIYNRTDTAQDRLANFDVILLDSSGKEIE"
     gene            12135..12647
                     /locus_tag="VW854_000668"
     CDS             12135..12647
                     /locus_tag="VW854_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HEV0261949.1"
                     /translation="MSAQITINHKKVRYVRIELEGYNALSLAEVEVFRFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGC
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            13301..14011
                     /gene="deoD"
                     /locus_tag="VW854_000669"
     CDS             13301..14011
                     /gene="deoD"
                     /locus_tag="VW854_000669"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV0261950.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHCVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKELGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(14034..14558)
                     /locus_tag="VW854_000670"
     CDS             complement(14034..14558)
                     /locus_tag="VW854_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEV0261951.1"
                     /translation="MKIAIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     HEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFSPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(14611..14847)
                     /locus_tag="VW854_000671"
     CDS             complement(14611..14847)
                     /locus_tag="VW854_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEV0261952.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLAK"
     gene            complement(14915..15835)
                     /locus_tag="VW854_000672"
     CDS             complement(14915..15835)
                     /locus_tag="VW854_000672"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEV0261953.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEEKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVEAFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            16033..16437
                     /locus_tag="VW854_000673"
     CDS             16033..16437
                     /locus_tag="VW854_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0261954.1"
                     /translation="MKILKRYILELCFILSFALPFIKGANADNGICFVETYYGFTFLM
                     EHAIVTAVFIYSFLIAFLLKKRWAKWIAAGSYCFLVLWIATEGYFFRMSLEDLIRLWT
                     SLEILTQTYQLGFYLNILLGILLIIKYFKVKQ"
     gene            16653..17234
                     /locus_tag="VW854_000674"
     CDS             16653..17234
                     /locus_tag="VW854_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV0261955.1"
                     /translation="MYYAENPDAAHDIHELRVELLGQKMTFLTDAGVFSKKIVDFGSQ
                     LLLKCLEVNQGETVLDVGCGYGPLGLSLAKAYGVQATMVDINTRALDLARRNAEKNNA
                     KATIFQSNIYEQVEGHFDHVISNPPIRAGKQVVHEIIEKSKDFLETGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            17231..18508
                     /locus_tag="VW854_000675"
     CDS             17231..18508
                     /locus_tag="VW854_000675"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEV0261956.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLVEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            18526..19188
                     /gene="deoC"
                     /locus_tag="VW854_000676"
     CDS             18526..19188
                     /gene="deoC"
                     /locus_tag="VW854_000676"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEV0261957.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASADKLVKVIIEACLLTDQEKIVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            19175..19564
                     /locus_tag="VW854_000677"
     CDS             19175..19564
                     /locus_tag="VW854_000677"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV0261958.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGNVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMVEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            19655..20707
                     /locus_tag="VW854_000678"
     CDS             19655..20707
                     /locus_tag="VW854_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEV0261959.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            20851..22386
                     /locus_tag="VW854_000679"
     CDS             20851..22386
                     /locus_tag="VW854_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0261960.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISESIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            22379..23437
                     /locus_tag="VW854_000680"
     CDS             22379..23437
                     /locus_tag="VW854_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0261961.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            23440..24396
                     /locus_tag="VW854_000681"
     CDS             23440..24396
                     /locus_tag="VW854_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0261962.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGVIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
     gene            24481..25829
                     /locus_tag="VW854_000682"
                     /pseudo
     CDS             24481..25829
                     /locus_tag="VW854_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            25958..27264
                     /locus_tag="VW854_000683"
                     /pseudo
     CDS             25958..27264
                     /locus_tag="VW854_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(27344..27985)
                     /gene="plsY"
                     /locus_tag="VW854_000684"
     CDS             complement(27344..27985)
                     /gene="plsY"
                     /locus_tag="VW854_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEV0261963.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            28121..30064
                     /gene="parE"
                     /locus_tag="VW854_000685"
     CDS             28121..30064
                     /gene="parE"
                     /locus_tag="VW854_000685"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEV0261964.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVVNTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            30484..32955
                     /gene="parC"
                     /locus_tag="VW854_000686"
     CDS             30484..32955
                     /gene="parC"
                     /locus_tag="VW854_000686"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEV0261965.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            33087..34109
                     /locus_tag="VW854_000687"
     CDS             33087..34109
                     /locus_tag="VW854_000687"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEV0261966.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGIGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            34165..34869
                     /locus_tag="VW854_000688"
     CDS             34165..34869
                     /locus_tag="VW854_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV0261967.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     STTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIIDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPVVSNVVPFSKPYSQVGIKGDPYIFKGMKLQKDILTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKHIK"
     gene            34934..35623
                     /locus_tag="VW854_000689"
     CDS             34934..35623
                     /locus_tag="VW854_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEV0261968.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            35620..36543
                     /locus_tag="VW854_000690"
     CDS             35620..36543
                     /locus_tag="VW854_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEV0261969.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            36558..37202
                     /gene="pcp"
                     /locus_tag="VW854_000691"
     CDS             36558..37202
                     /gene="pcp"
                     /locus_tag="VW854_000691"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEV0261970.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            37370..37600
                     /locus_tag="VW854_000692"
     CDS             37370..37600
                     /locus_tag="VW854_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEV0261971.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            37650..37730
                     /locus_tag="VW854_000693"
     tRNA            37650..37730
                     /locus_tag="VW854_000693"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37684..37686,aa:Tyr,seq:gta)
     gene            37740..37811
                     /locus_tag="VW854_000694"
     tRNA            37740..37811
                     /locus_tag="VW854_000694"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37772..37774,aa:Gln,seq:ttg)
     gene            37973..39175
                     /gene="rpsA"
                     /locus_tag="VW854_000695"
     CDS             37973..39175
                     /gene="rpsA"
                     /locus_tag="VW854_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEV0261972.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
BASE COUNT        11825 a         7038 c         8786 g        11608 t
ORIGIN      
        1 atcataatgg acaaagggca agctgtcaag aaattacagt tattttagta aaaaaagaag
       61 cagaaaaatt tcattttcta ctcattttct ataggattta tttttctttt tcagaacttt
      121 taacttcagc ttttttggca gatttaatct gaatgtttcc cttgctagtc ataactgctt
      181 tgagatcttt ttcgcttgga ttttcaaggt agtagtcagt gattgtgtcc tcaatatagt
      241 cttgaatagt ccgacgaagt gggcgtgctc ccatttttgt atcataacct aggtcaacca
      301 acttttcctt gaccttatca gttacatcca aacgaatgtt gttgctagag aggcgcttgt
      361 taacatctgc tagcatgagc tcgacaatct gaaggaggtt atccttgctg agagccttaa
      421 attcgataat gccatcaaaa cggttcataa actctgggct aaagaagtta ccgagttcac
      481 cgaggacaga attggtacgt ccttctctag cagcaccaaa tccaacgcta gcttcggtct
      541 ttcctgtacc tgcatttgag gtcatgataa tgatggcatc cttgaagcta acggtgcgtc
      601 cttgcccgtc tgtcaaacga ccatcgtcca agacttgaag aaacatgtgc ataacatctg
      661 ggtgagcttt ttccacttca tcgagaagga tgagagaata tggattgtgg cgaacttttt
      721 cagttaattg accagcctca tcatagccaa cataacctgg aggggcgccg accaacttag
      781 ccacactatg tttttccatg tattcactca tatcaaagcg aatcatacta tcagcagaac
      841 caaaaagttc gatagccagt tgtttggaaa gttctgtctt accgacacca gttggcccaa
      901 cgaagaggaa gcttccgatt gggcggttag gggtaccaag tccgacacga ttacggcgaa
      961 tagccttggc aatcttatcg actgcatcat cttggccaat aacatgagac ttgagatctt
     1021 cggctagatg gatgagttga gattgttctt tctctttcaa atcaccaaca gggatattgg
     1081 ttttctgctc gataatgtgc tcaatagttt tctcgctgat gataggagta tcctggtctg
     1141 tgatcttttt cttttgcatt tccttatact tggcaatctg gtcgcggaag taggccgcct
     1201 tctcaaaatc ttcttctcgt gtagcttgag acttgagatt ttcagcctca atcaagcgct
     1261 gatcaattac tttaggatcc acaaaattca aggtcaagtt catcttagaa ccagcttcat
     1321 ctaggaggtc aatggccttg tcaggcaaga agcgatcttg gatgtagcga ttggaaagag
     1381 ttgcagctgc ttcaatcgca gcatctgtat attgaacgtg gtggtaatct tcgtatttct
     1441 tttgaatccc tttgagaata gtgattgttt cgtccaccgt tggttcatcg actttaacag
     1501 gctgcatacg acgctcgagg gcagcatcct tttcaatgat acggtattca ttgagggtag
     1561 tagcaccgac tagttgcagt tctccacgag caagggctgg cttgaggata tttcctgcgt
     1621 ccatattacc atcactcgca gaaccagcac caacaatttc atggatttca tcgataaaga
     1681 ggatgatgtc ttcacgtttg cgaatttctt ccatgagttt ttgcatgcgt tcttcaaatt
     1741 gtcctcgaat ccccgttcct tgaactaagc taaccacatc cagacggatg acttgtttac
     1801 cttggagttt atgtggcaca tcgccatcga caattttctg agctagacct tcgacaacgg
     1861 ccgtttttcc gacaccaggt tcaccgataa ggacaggatt attcttggtt ctacgattga
     1921 gaatctcgat gacacggata atctcatcgt cgcgcccaat aacggggtca atgtctccac
     1981 gacgggcaat ttcagttaca ttaataccaa attcttccag caggcctttt tcttggctag
     2041 gtggcggagt ttgagcagat ccacgatttt gggaaccata accgccgttt ccaccgtaac
     2101 ctccacctga ttgggttggg ggaataggag gagtattgct agaaggtctg aaattgttta
     2161 gatcattgaa gaaatcacca aagggatcga agtcacgatt gttcagatcc gtcatacctt
     2221 tgaagaggct attgttagga tctgttttga taatcttata gcagttttga cagaggtcaa
     2281 tttgtttttg ttttccattg agattggtgt aaagatgaat tgttgagtcg ttaattttac
     2341 agttttgaca aagcatacat ctacctcatt tctttcttta gcctgacttg tttaaaggtc
     2401 aaaaatagtc aaattctata ctctcattat aacaggattg gggtgtaaag caagtaaaaa
     2461 gattgaagct ttgagaagtt gttacaatga aaatttttgt gaatttggac tataaaaaaa
     2521 tcctatttgt gagacaaaaa ggattttact tagacatgca gggacaatcc cagcttgttt
     2581 tagattagta gagaagttcg taaatttcca ttgcgattaa gtcaatgcta tcaaacgtat
     2641 acgtttcgcg atcttctacg tcacgaaggg taaagattgg gccgttttct tcgtcgtggc
     2701 tgtacgcaac ttctgcaaca acaactcctt cgcgttcaaa attacgtttt aaatttccgc
     2761 catcgtttgc cattgcttca aggcggttga tgattctaac caaatgtgat tccattttga
     2821 ttctccttca tttcttatcg ttactatttt accataaagg aggccaactt gactagaaaa
     2881 aactaagatt tctcgctaat tctgctagtt taaagatttt taaataaatt ggataaaaaa
     2941 tttaaatttt aattcaaaac atgttataat catacagtat tctattttag aaagcagtgt
     3001 gactatgaat ttttcttttt tacctaagta tttaccttat tttaactatg gggctgttgt
     3061 gacgattctt atttctatct gtgttatctt tttgggaact attttgggtg ttgtcttggc
     3121 ttttgggcaa cgttcaaagt ttaaaccgct tgtttggttg gccaacttgt acgtttggat
     3181 tttccgtggg acaccgatga tggttcaaat tatgattgcc tttgctctta tgcatatcaa
     3241 tgctccgact attcagattg gaattttagg tgttgatttt tcgcgtctga ttccagggat
     3301 tttgattatc tctatgaata gtggtgctta tgtttcggag attgttcgtg ccggaatcaa
     3361 tgcggttcca aaaggtcagc tagaggcggc ttattcgcta gggattcgtc ctaaaaatgc
     3421 gatgcgttat gtgattttgc cacaagcagt caaaaatatc ttgccagcat tggggaacga
     3481 atttatcacc attatcaagg acagctccct cttatcagct attggggtca tggagttgtg
     3541 gaatggggct acaacagttt ctacaacaac ctatctacct ttaacaccac ttttatttgc
     3601 agcattttac tacttgatta tgacctctat tctgacagta gccttgaaag cttttgaaaa
     3661 acatatggga caaggagata agaaataatg acagaaacct tgataaaaat tgaaaattta
     3721 cataaatctt ttggaaagaa tgaagtattg aagggcatca acctcgagat taaaagagga
     3781 gaagttgtcg ttatcatcgg tccttcaggg agtgggaaat ctaccttgct tcgctctatg
     3841 aatttgttgg aggaagcaac caaggggaag gttatctttg agggagtcga tattacggac
     3901 aagaagaatg acctgtttgc catgcgtgag aagatgggca tggttttcca acaatttaac
     3961 ctctttccta atatgactgt gatggaaaat attaccttgt cacctatcaa gactaaaggt
     4021 gaaagcaggg aagttgctga gaagagagcc caagagcttt tggaaaaagt tggtttgcca
     4081 gataaggcag acgcttatcc acagagtctg tcgggtgggc aacaacaacg gattgccatc
     4141 gcgcgtgggt tggctatgga accagatgtt ttgctctttg acgaaccaac ttcagccttg
     4201 gatcctgaaa tggtaggtga ggtattggct gttatgcaag accttgccaa gtcagggatg
     4261 actatggtta tcgtaacaca tgagatggga tttgcccgtg aggtggcaga tcgtgttatc
     4321 tttatggcag acggtgtggt tgttgaagat gggacacctg agcagatttt tgaacaaacc
     4381 caagaacaac ggactaaaga tttcttgagt aaggttttat aatctatttt aaaatttcaa
     4441 gacaagatta gtgaaaagtt caaccagagc tttttcttat agtttgaaac tataggataa
     4501 cctaggaaag aagtgttaga gggcactagc agtttttggt ataatggaag atatttgaaa
     4561 gaaaagagaa gtgatatgac acagattatt gatgggaaag ctttagcggc caaattgcag
     4621 gggcagttgg ctgaaaagac tgcaaaatta aaggaggaaa cagctctagt gcctggtttg
     4681 gtagtgattt tggttgggga taatccagcc agccaagtct acgttcgcaa caaggagagg
     4741 tcagctcttg cggttggttc ccgtagcgaa gtagtgcgag ttccagagac cattactcaa
     4801 gaggaattgt tagacctgat tgctaaatat aatcaggatc cagcttggca tgggattttg
     4861 gtccagttgc cattaccaaa acatatcgat gaagaggcgg ttttattaac cattgaccca
     4921 gaaaaggatg tggatggttt ccatccccta aacatgggac gtctttggtc tggacatcca
     4981 gtcatgattc cctcgacacc tgcaggaatt atggaaatgt ttcatgaata tgggattgac
     5041 ttggaaggta aaaatgcggt cgtaatcggt cgttcaaata tcgttggaaa acctatggct
     5101 cagcttcttt tggctaaaaa tgcgacagta accttgaccc actcacgaac tcataatctt
     5161 gccaaggtgg ctgctaaagc agatattctt gtagtcgcaa tcggccgcgc caagtttgtg
     5221 actgctgact ttgtcaaacc aggtgcggtt gtcattgacg ttgggatgaa tcgagatgaa
     5281 aatgggaagc tctgtggaga tgttgattat gaggcagttg caccacttgc tagccatatc
     5341 acgccagtcc ctggaggtgt cggtcctatg accattacca tgctgatgga gcaaacttat
     5401 caagcagcac ttcggacatt ggatagaaaa taagagaagc attcgattaa tagagtgtat
     5461 tttctatagc tatatctaaa atggcagaaa tgaatattaa attttagata taagattaca
     5521 aaaggaggtc tgcgtctcct ttttattgta taataaaagt gagaggaaaa aaggatgaaa
     5581 gtgattaatc aaaccttatt agaaaaagtt attattgaac gttctcgttc aagtcataaa
     5641 ggagattatg gtcgtctgct gctgcttggt ggtacttatc cttatggagg tgccatcatc
     5701 atggctgctt tagcagctgt aaaaagcggt gcaggattgg tgaccgttgg aacggacagg
     5761 gaaaatatcc cggctctgca cagccattta cctgagccta tggccttttc tcttcaagac
     5821 cagcaattgt taaaagagca attggagaag gcagaagtta tcttgctggg gcctggttta
     5881 ggagacaatg cttttggaga agatttagtt aaacaggtct ttgctggctt aaaacagaat
     5941 cagattttga ttgtagacgg aggtgccttg accattcttg ctaggacaag tttgtcattt
     6001 ccctcaagcc agcttatcct aactcctcac caaaaagaat gggaaaaatt atctggaatt
     6061 acgattgaaa agcaaaagga agatgcaacg gctagtgttc tgacttcctt ccctcaagga
     6121 acaattttgg tggagaaagg cccagctact cgtatttggg aagttggcca atctgattat
     6181 taccagttac aggttggtgg tccttatcag gcgactgggg gaatgggaga tactctggct
     6241 gggatgatcg caggatttgt aggccaattc cgacaggcca gtctctacga gcgtgtggca
     6301 gtagcgaccc atcttcattc agccatagcc caagaactat ctcaagaaaa ttatgtggtc
     6361 ttaccgacgg aaatcagcag gtatcttccc aaaataatga aaattatttg tcaacaagag
     6421 agggtaagca aagataaact tgtttaaaat agacagataa atctacttcc ttctcttaat
     6481 ttatgattct tgaaatgcgc ctgctttaag gctaaactag acgtatcaaa gttaagagtt
     6541 tgtttttgta actggaaaga gatgaacaaa tcaaatacat aaaagataaa ctcattttta
     6601 ttcgtttggt aaaaataaaa tgcatatttt taaagaaaga ttgatgaaaa atgtagattt
     6661 gatgttttta cttgaataaa aagcggtttc atagtaaaat aagaactgag aatgatggag
     6721 gtaaatcggt ggaaaatctg aagaaaatgg caggtatcaa ggctgctgag ttcgtgagcg
     6781 atggaatggt cgttggactt ggaacaggct cgactgccta ttattttgtc gaagaaatcg
     6841 gtcgtcgtat caaggaagaa ggattacaga ttacagctgt aacaacttct agtgtgacca
     6901 ctaaacaggc agaaggcctt aatatcccgc tcaagtctat tgaccaagta gactttgtcg
     6961 atgtgacggt tgatggcgcg gatgaagtgg atagtcagtt taatggaatt aaaggcggtg
     7021 gtggtgccct tctcatggaa aaggtggtcg caacaccatc aaaagaatac atttgggtgg
     7081 tggatgaaag caagctggtc gaaaaactag gtgcttttaa attgccagta gaagtggttc
     7141 agtatggtgc agagcaggtc tttcgtcatt ttgaacgagc tggctacaaa ccaagtttcc
     7201 gtgaaaaaga cggccaacgt tttgtgacgg atatgcagaa ttttatcatt gacctcgcct
     7261 tggatgtcat tgaaaatcca attgcttttg gacaagaatt ggaccatgtc gttggtgttg
     7321 tggagcatgg tttattcaac caaatggtgg ataaggtcat cgttgctgga cgagatggag
     7381 ttcagatttc aacttcaaaa aaaggaaaat agaagggggc ataagatgtc taaatttaat
     7441 cgtattcatt tggtggtact ggattctgta ggaatcggtg cagcaccaga tgctaataac
     7501 tttgtcaatg caggggttcc agatggagct tctgacacac tgggacacat ttcaaaaaca
     7561 gttggtttga atgtcccaaa catggctaaa ataggtcttg gaaatattcc tcgtgaaact
     7621 cctcttaaga ctgtagcagc tgaaagcaat ccaactggat atgcaacaaa attagaggaa
     7681 gtatctcttg gtaaggatac tatgactgga cactgggaaa tcatgggact caacattact
     7741 gagcctttcg atactttctg gaacggattc ccagaagaaa tcctgacaaa aatcgaagaa
     7801 ttctcaggac gcaaggttat tcgtgaagcc aacaaacctt attcaggaac ggctgttatc
     7861 gatgattttg gaccacgtca gatggaaact ggagagttga ttatctatac ttcagctgac
     7921 cctgttttgc agattgctgc ccacgaagac attattcctt tggatgaatt gtaccgtatc
     7981 tgtgaatacg ctcgttcgat tacccttgag cgtcctgccc ttcttggtcg catcattgct
     8041 cgcccttatg taggtgaacc aggtaacttc actcgtacgg caaaccgtcg tgacttggct
     8101 gtatctccat tttccccaac tgttttggat aaattgaatg aggctggtat cgatacttat
     8161 gctgtgggta aaatcaacga tatctttaac ggtgctggta tcaaccatga catgggtcac
     8221 aacaagtcaa atagtcatgg aattgataca ctattgaaga ctatgggact tgctgagttt
     8281 gaaaaaggat tctcattcac aaacctagtt gactttgatg ccctttacgg ccatcgtcgt
     8341 aatgctcacg gttaccgtga ttgcttgcat gagtttgatg aacgcttacc tgaaattatc
     8401 gcagctatga gagagaatga ccttctcttg attactgcgg accatggaaa tgacccaacg
     8461 tatgcaggaa cggatcacac tcgggaatat attccattgt tggcctatag ccctgccttt
     8521 aaaggaaatg gtctcattcc agtaggacat tttgcggata tttcagcgac tgttgccgat
     8581 aactttggtg tggaaactgc tatgattggg gaaagtttct tagataaatt ggtataagat
     8641 gacgcgctat gctttgctgg tgagaggtat caatgttggt ggtaagaata aggtcgtcat
     8701 ggcggagctt cgtcaagaat tgacaaactt gggactggaa aagtttgaga gctacatcaa
     8761 cagtggcaat attttcttta cttcgataga ttccaaagcc caattggttg aaaagctaga
     8821 gactttcttt gcagtccatt atccatttat tcagagcttt tctttactga gtctagagga
     8881 ctttgaggcg gaacttgaaa atctaccagc ttggtggagc agagacttgg cacgaaaaga
     8941 ttttctcttt tacactgagg gtttggatgt ggaccaagtc atcgcgacag ttgaaagttt
     9001 agagctgaaa gatgaagtgc tttattttgg aaaacttggg attttctggg ggaaattttc
     9061 tgaagaatcc tattctaaga ctgcctatca taagtacttg ctgaaggtgc ctttctaccg
     9121 ccacattact attcgtaatg ctaaaacctt tgacaaaatt ggtcaaatgc taaaaaaata
     9181 ataaaggaga cacacaatga catttttaaa caaaatccat gaaactgcta ctttcctgaa
     9241 agaaaaggga attgcagccc ctgagttcgg tctaatcctt gggtcaggac ttggagaatt
     9301 ggcagaagaa atcgaaaatc cagttgtagt agactatgct gagattccaa actggggccg
     9361 ttcaacagta gtcggtcatg ctggtaaatt ggtatatggt gaactggcag gtcgcaaggt
     9421 cttggctctt caaggtcgtt tccatttcta tgaagggaat cctctggaag tggtgacttt
     9481 cccagttcgt gtgatgaaag ttcttggatg tgaaggtgtt attgtaacca atgcagctgg
     9541 cggtatcgga tttggtcctg gtaccttgat ggctatctca gaccatatca acatgacggg
     9601 gcaaaatcca ttgatgggtg aaaacttgga tgactttggc ccacgtttcc cagatatgtc
     9661 tagggcctac acaccagaat accgtgccac tgcccatgaa gtggctaaaa aacttaatat
     9721 caagcttgat gaaggtgtct atatcggagt tactggtccg acttatgaaa caccagcaga
     9781 aattcgttcc tataagacac tgggagcaga tgcagttggt atgtctacgg ttcctgaagt
     9841 tatcgtggca gcccactctg gcttgaaagt tctgggaatt tcatgtatca ctaactttgc
     9901 ggctggtttc caagaagaac tcaatcacga agaagttgta gaagtgactg aacgtgttaa
     9961 aggtgatttc aaaggcttgc ttaaagcgat tcttgctgaa ttgtaagaaa aaagatttaa
    10021 aaggggaagt gcctctgttt tttcaggatt gactgcctat ccggattaaa gaagaaacag
    10081 aggaatacta tgagcttctt cctgctctta taactgaaag aagcggaaga ataggtatgt
    10141 ctgatctgat agccagcatt gtgaaagaca agattctagg atactagcat tagctttcta
    10201 gccaagcaga ctagtatgat aaggagagat gagaatgaat tgactttctg aatttctcag
    10261 tcttatcata tatagcacaa tgagatttcg cttgagtctg cttgtaaata aacgaaaaga
    10321 aagataagaa ataatgaaaa ttggtcaacg aattatgcgc tttggcataa aaaattaagt
    10381 atcggagttg tatctgttgt agtcggcttt gatttctagc tccagctgga atttcagcca
    10441 atgaagtaaa gcaagatgta acatttgaag tggtaatagg tgtgctagat tctaaggagg
    10501 aattgaaaga gtcagaaaat gatgctccaa aactagaaac tcctcttaga gaggagccaa
    10561 gactagctcc tcaaacgctt ccggaagcaa gtgaagttct tgaaaacaaa agggaagagt
    10621 caaaagtaga gataacagag ccagctcaag actcacctat tcttgctcct gtagaagaaa
    10681 ctaaagaaga agctgtgaca gaaaaaccaa caaatactcg ttctctaact gcagaagatt
    10741 tggtgaagat ttccaaaggg gaattgcatt tagaaaatga tttgattgat gaatctttct
    10801 atggtgaaaa agctcttgat ttggaagggg atgatgacca ggatggcatc aaaaacaaag
    10861 atggtaagga ttatctagga tataacagtc atcccttgct agcagacagt gatggggatg
    10921 gtttggcaga tggggaagat gataataaga aagaatggta tgtcacagac cgtgattctc
    10981 ttctctttat ggagttagct tatcgagacg atgattatat tgagaaaatt ttatatcata
    11041 agaatctttt ccctagtctc tatcttgacc gtcaagaaca caaactcatg cacaatgaat
    11101 tggctccttt ctggaagatg aaaaaagcct actatacaga tagtggcttg gatgctttct
    11161 tatttgagac caagagcgac cttccttatc tcaaagatgg aacggtgtac atgttggcta
    11221 ttcgtggaac gcgagttaat gacgccaagg acttgagtgc agatttggtt ttattaggtg
    11281 gaaataaacc agctcaagcg gatgatatcc gcaaggttgt tggggaatta gccaaggata
    11341 taagtattac taagttgtat atgacaggtc attctcttgg aggctaccta gctcagattg
    11401 cagcggttga agattaccaa aaatatcctg atttttataa ccatgtattg aggaaagtga
    11461 caactttcag tgctcctaaa gtcattactt ccagaactgt ttgggatgct aagaatggtt
    11521 tctgagatgt tggtttggaa agtcgtaaat tagctgttag tggaaaaatt aagcattatg
    11581 tggttgataa tgacaatgtt gtgactccct tgattcataa taatcgtgat attgttacat
    11641 ttacaggtaa ttcacgcttt aaacaccgtt ctcgtggcta ttttgaaagt ccaatgaatg
    11701 atattcctaa ctttaatatt ggtaaacaag ctaccttgga taaacatggt tatcgtgatc
    11761 cgaaattgga taaagtgcga ttctttaaga aacaggctct gcctcgatct tttagtcaac
    11821 caagcgctga accaatggaa aatattgcct taggaaaaca ggttactcaa agttcgacag
    11881 ttttcggagg agatgctaga agagctgtgg atggcaaagt cgatggtaac tatggtcaca
    11941 attctgtcac tcatacaaac ttccaatcta agccttggtg gcaagtagat ttggctaaag
    12001 aagaaaccat tcgccaaatc aatatttaca accgaacaga cactgcccag gatagattgg
    12061 caaactttga tgtcattctt ttagacagtt ctggtaaaga aattgagtga aaacgtataa
    12121 catctcctaa agatgtgtca gcacaaatta cgattaacca taaaaaagtg cgctatgttc
    12181 ggattgagct agaaggctat aatgccctca gtcttgcaga agttgaagtt ttccgcttta
    12241 tagctacgaa tgctgaaacg gcgacacaag tttctaagcc agttcaacca atcagtcaga
    12301 ctcctgtgaa agataaaaca ttgacaattc aacacagtgg agcttacatt gcccgctact
    12361 ccataacttg ggaagaagtt ccagtagata aagatggaaa ccaagttgtt cgtagtcatt
    12421 cttgggaagg aaacggttgc aaccagactg caggttttgt cctcaacctc ccaatcaaag
    12481 aaaatatgag aaatctgcga gttaagattg agaaaaagac gggcctacta tggaatagat
    12541 ggcaaacaat ctatgaaaac agaccaattt tagctcaacc ccaccgtaaa attacccatt
    12601 ggggtacgac attgaattcc aaggtgagtg acgatgatgt cttgtaatct gatggtacaa
    12661 tgacagttag tttgtctagt ttataagaaa gtactacctg agcttgaata gaactcaggt
    12721 agctctctgt gaaagaacaa aattaatact caatgaaaat caaagagcaa actaagaaac
    12781 tagccgcagg ttgctcaaag cactgctttg aggttgtaga taagactgac gaagtcagtc
    12841 acatatataa tccaaggcga cgttgacgtg gtttgaagag attttcgaag agtataaaca
    12901 gaaaggtaga gcgcgtgttc taatttgaac acgagtagaa aacttttcta aaaacaaaaa
    12961 cgaaaggatg ggtaaactgt attcgctgaa ctgaatacgg gcgactctcc tctaaatcaa
    13021 aattaagaaa ggaattgacc ccaccctaaa agtagtggga aaaagatagt tgatctagcg
    13081 agcatcgctc actgcgccca actcctattt tcccttcgct ttttgatggg tttggtatct
    13141 ttctcaatat aaaatataaa ataaagaaag gtagagcgtg tgttttgatt tgaacacgag
    13201 cggaaaactc ggaaaataga taatctgact gaaaaatcag gatttctcgt caggttccta
    13261 attttcagtc gttttcttct cgctctttgt atcataaatt atgtctatcc atattgctgc
    13321 tcagcagggt gaaattgctg ataaaattct tcttcctggg gatcctcttc gtgctaagtt
    13381 tattgcggag aatttccttg atgatgctgt ttgttttaac gaagtgcgta acatgtttgg
    13441 ttacactggt acttacaagg gtcactgtgt atctgtcatg ggaactggga tgggaatgcc
    13501 atctatttcg atttatgcgc gtgagttaat cgtagactac ggtgtgaaga aattgattcg
    13561 tgtgggaact gcaggttctt tgaatgaaga ggttcatgtt cgtgaattag ttttggcgca
    13621 ggcggctgca accaactcaa acatcgttcg taatgactgg ccacagtacg attttccaca
    13681 aattgctagc tttgatttgc ttgataaagc ctaccatatc gccaaagaac ttggtatgac
    13741 tactcacgtt gggaacgttt tgtcatctga tgtcttttac tcaaattact ttgaaaagaa
    13801 tatcgagctt ggtaaatggg gagtcaaggc tgtggaaatg gaagcagcag ctctttacta
    13861 tcttgctgcc caataccatg ttgatgcgct agctatcatg accatctctg atagcttggt
    13921 caatccagac gaagacacaa ctgcagaaga acgtcaaaat accttcactg atatgatgaa
    13981 ggttggtttg gaaaccttga ttgcagaata attatagcca aaaaggaagc cctttatgaa
    14041 ttgtaggact tccttttctt atccagaaat tgatctagct ctctctgatt tcgaagaata
    14101 gtgactttat gtgaatattc ttggcaaagt ttttggtaat tttctttttg agttttgcta
    14161 cgcccatccc aaagaatcca tctgataaac tcccactcaa agcgttcagg gcaatctgcc
    14221 gccatacttt ctctgacttt tccacggtat ttaagataac gcttaaaggc tctaaagaga
    14281 caggtcaatg gcgaaaaatt gagaaagatg atttggtcag cttcttgcat tcgttcttgg
    14341 tagtagcacc aagaataatt accatcgatg acccaagctt tatgcttggt gagaaagttt
    14401 tttatctcgg ttaacatcca ttcgtggtca ctgtcttgcc aaccaggttg aaattggagt
    14461 gtgtccatgt gcagttttgg aatggagtag tagttagata acttttctgc tagagttgac
    14521 ttaccagaac cagaatatcc gataattgcg attttcattt tctacctttt cctatttgga
    14581 gacaaaaaaa cagcctctat ggactgtttc ttatttagca agtttagctg aaagacgagc
    14641 tttatcgcgg cttgctttgt ttttgtgaat caaaccttta gtttctgctt tatcgatagc
    14701 tgagctagca gcacggaaaa gttcttcaga tgggtttgct tcgaaagctt ttatagcagt
    14761 acgcatagct gatttttgag ctgagttctt ttcgttttgt ttaacgttca attcagcgcg
    14821 tttgatagct gatttaatgt ttgccaatgt tcttacctcc atatttacta actataccat
    14881 tatatatgaa aacttacgtt ttgacaaggg aaaattactt ttttaagtaa atttcatcga
    14941 tttcatggtt cttgctttta tgaagaatca cttctgcacg atttctggtt ggttcaatat
    15001 aattttgcag atttgtgaga ttgatactga tccagacctg atgggcaaag gcttccactt
    15061 ccccaatcgg catctgagta aaacgataat agtagctatc agggtcgttt tgggctagac
    15121 tcagcatttt caagaaacgg tccagatacc aactttcaat atcatccact ccagcatcta
    15181 catagatgga aaagtcaaag aagtcagtga tatagagacg atcgttttgt ggattttgaa
    15241 agacattgat tccctcaaca attacaaaat cagcagcttt gacactttgt ttctcttcgg
    15301 gtacgatgtc gtaaacttca tgagaataga caggaatatc tacatcttgt ccatttttga
    15361 tgcggtccaa gaagttgaga agagcttcca tatcatagct ttcaggaaat cctttacgat
    15421 ttaaaatccc ctgctcaatc aaggtttgat tgggatagag aaaaccatca gttgtaacca
    15481 actcaaccgt agcatctgta aacgtacggg acagtaggat ttgaagtagg cgactggttg
    15541 tggattttcc aacggcaaca ctcccagaaa ccccaataat aaaaggttga gatttacttt
    15601 cacgttggag gaaaattcct tttgaaaagg ctaaatcttc cttagttcgc ttgtaaatct
    15661 gaatcagatg agctaagggg agatagatat ctgtaacgtc ttggagactg atttggtcat
    15721 taaaactctt gatagattcc aattcttctt ctgtcaaagg aggtgttgtc tttcgatgta
    15781 aagattgcca agtctggcgg ctgatttttt caaaatgtaa aaattcgttg gtcatttgtt
    15841 ttcccctaga tattttgtct atcataccat aaaaatctaa aaaaataaag cggtttcttg
    15901 aatgtggtaa gtcaatggta tatagtataa gtgatcaatt tattgatgaa ataggagata
    15961 gattctactg atttataagt ttataagctt ataaaggggg ctttttagtt cacaacttat
    16021 aggaggtgta ctatgaaaat cttaaaacgt tacatattgg aactctgttt tattttaagt
    16081 tttgctttac cttttataaa aggagccaat gcagataatg gtatatgctt tgtggaaacc
    16141 tattacggtt ttactttttt gatggaacat gctattgtaa cagctgtctt tatctattcg
    16201 tttttaattg ctttcttact aaaaaaacga tgggcgaaat ggattgctgc tggtagttat
    16261 tgctttttag tcctatggat tgcgacagag ggttatttct tccgcatgtc actagaagat
    16321 ttgatacgac tttggacaag tttggaaatc ctgacacaaa cgtatcagtt gggcttttat
    16381 ctaaacattt tgttaggaat tttgttgata ataaagtatt ttaaggttaa gcaatagaaa
    16441 taataatctg cctgattata gatgataaac tttttgtttt tatctggact gtgatattta
    16501 tagttctaaa tggtttatta tttataagtg ttgaaagaga ttgaagaaaa tggcttcgtg
    16561 acttgctttc ttcggtcttt ttcgttttat tatcaatttg taaacgattt acaatttgag
    16621 tcaaattatg gtaaaatagt gtcatgagta aaatgtatta tgcagaaaat cctgacgctg
    16681 ctcacgacat tcatgagttg agagtagagt tgttgggaca aaaaatgacc tttttaacgg
    16741 atgcgggtgt ttttagcaag aaaatagttg actttggaag tcaactattg ctcaagtgtc
    16801 ttgaggtcaa ccaaggagaa actgtccttg atgtaggctg tggttacgga cctttgggtt
    16861 tgtccttagc taaggcttat ggagttcagg ctactatggt tgatatcaac actcgtgcct
    16921 tggatttagc tcggagaaat gctgaaaaaa ataatgcaaa agcgacgatt ttccagtcca
    16981 acatctatga acaagttgaa ggacattttg atcatgtcat ttccaatcct cctatccgtg
    17041 cgggcaagca agtcgttcat gaaatcattg agaaaagtaa agatttcttg gaaactggtg
    17101 gggatttgac aatcgtcatc cagaaaaaac aaggggctcc aagtgctaag tccaagatgg
    17161 aggatgtgtt tggtaattgt gaaatcctta aaaaagacaa gggatactat atccttagaa
    17221 gtgtgaaaga atgagagcag ttgatttaat acaaaaaaaa cgagacggtc aagaactgac
    17281 ttcaagtgaa attgaatggc tggtagaagg ctatgtgtca ggaactgttc ctgattatca
    17341 gatgtctgcc tttgctatgg ctgtttattt taaaggaatg acgactcgtg aaatttctga
    17401 tttaacgatg aatatggtta agactgggca agagtttgac ctgtcagcca ttgatggtgt
    17461 gaaagttgac aagcattcta ctggtggtgt cggcgataag gtaaccttga ttctagctcc
    17521 tcttgttgct agcttcggtg tgcctgtagc aaaaatgagt ggtcgtggtc ttggccatac
    17581 aggcggaaca attgataaat tggagtccat taagggctat caagtagaac gtagtcaaga
    17641 ggattttatt cgtcaggttc aggatattgg agtatctgtc attgggcaat cagaccagct
    17701 ggtcaaagca gataagcttc tctatgccct tcgtgatgtg accgcaactg tcgacacgat
    17761 tcctttgatt gcgagttcgg tgatgagcaa gaaaattgcg gcaggggcgg atgctatttt
    17821 gctagacgtg actgtcggtg agggtgcctt catgaagacg gttgatgagg cgcgtgagtt
    17881 ggctcaaact atggtagatc ttggtaaggt tgttggacgg aagacagtag cagtgattac
    17941 ggatatgagc cagcctttgg gacgagcgat tggaaatcgt ctggaaatcc ttgaagcatt
    18001 ggagattttg caaggtcaag gacgtcagga tattacccac tttatttgtg aattggctca
    18061 aattatgctt ggtttggcaa atgttaacaa gactgttgaa gaagttcgcc aacatcttga
    18121 gaatggtcaa gcactggcta agtttgagga aatggtgcaa gcccaaggtg gagacttgga
    18181 agacctctat cgtcctgtaa atgtagctca tgtggtggaa atccctgctc aggaaacggg
    18241 tgtcatttca gctcttccag ctatggactt tggtctttat gccatgaggc tgggtgctgg
    18301 tcgtgcagtt aagtctgatg ccttggacta tgaaacagga attgtttttg aaaagaagat
    18361 tggagactcc gttcaaaagg gtgaaattgt tgcaaaagta tacacaaatg aaaaaattcc
    18421 acctcaacta gttacagatt tccaaaaatg tgttaaaata agtgatgaag tgaaaaaaat
    18481 ccgtgaaatt gtagaaatca tctcctaaat ggagggaaaa ccgaaatgaa attaaataaa
    18541 tatatagatc atacgctttt aaaacaagat gcaaagaaaa aacaaattga tagtttgttg
    18601 tctgaggcta gagagtatga ctttgccagt gtttgcgtta atccgacctg ggttgaacat
    18661 gctaaaaaag gacttgaagg cacagatgtt aaggtttgca cagtagtagg tttccctttg
    18721 ggagcaacaa cttcagccgt gaaagcattt gagacaaaag aagctatcca aaatggtgca
    18781 gatgagattg atatggtgat caatgttgga gctctcaaat caggtaattt agccttggtt
    18841 gagtcagata ttcgcgcagt agtggaagca agtgctgata agttagtgaa agtcattatt
    18901 gaagcttgcc ttctgacaga ccaagaaaaa attgttgttt gccaattggc ccaaaaagct
    18961 ggggctgact ttgttaaaac atctactggt ttttcaactg gtggtgctac gatagcagat
    19021 gttacattaa tgcgtgaaac agttggatct gatatgggag tcaaggctgc tggtggagct
    19081 cgttcttatg cagatgctct tgcctttgtc gaagcaggtg cgacccgtat cggaacgtca
    19141 gctggggtag ctattttaaa aggagaattg gcagatggcg actactgagt tgattgaact
    19201 ggcaattgaa accagcaaac atgcctatgt cccctattct cactttccta tcggagcagt
    19261 tttagtagcc aaagacggaa atgtttacac gggagtgaat atcgaaaatg ctagctatcc
    19321 tttgaccaat tgcggtgaga gaacagctat ttttaaggct atatctgaag gccaaagaga
    19381 attttcagaa ttgattgtct atggtcaaac tgaaaaaccg atttcaccat gtggtgcttg
    19441 tcgccaagta atggtcgaat tttttgaaca agatttaaaa gtgaccttag tcgcaaaaga
    19501 taaatcgacg gtcgagatga cggtcgggga gttacttcca tattctttta cagacttaaa
    19561 ttagtctgag tcgctctttg agtggcaagg gtctttgtga ccaatcaatc catacttgca
    19621 acatcgttgc acatcttatt taggaggttc agtaatgaac aagaaacaat ggctaggtct
    19681 tggcctagtt gcagtggcag cagttggact tgctgcatgt ggtaaccgct cttctcgtaa
    19741 cgcagcttca tcttctgatg tgaagacaaa agcagcaatc gtcactgata ctggtggtgt
    19801 tgatgacaaa tcattcaacc aatcagcttg ggaaggtttg caggcttggg gtaaagaaca
    19861 caatctttca aaagataacg gtttcactta cttccaatca acaagtgaag ctgactacgc
    19921 taacaacttg caacaagcgg ctggaagtta caacctaatc tttggtgttg gttttgccct
    19981 tcacaatgca gttgaagaag cagctaaaga acacactgac ttgaactatg tcttgattga
    20041 tgatgtgatt aaagaccaaa agaatgttgc gagtgtaact ttcgctgata atgagtcagg
    20101 ttaccttgca ggtgtggctg cagcaaaaac aactaagaca aaacaagttg gttttgtagg
    20161 tggtatcgaa tctgaagtta tctctcgttt tgaagcagga ttcaaggctg gtgttgcgtc
    20221 agtagaccca tctatcaaag tccaagttga ctacgctggt tcatttggtg atgcggctaa
    20281 aggtaaaaca attgcagccg cacaatacgc agccggtgca gatattgttt accaagtagc
    20341 tggtggtaca ggtgcaggtg tctttgcaga ggcaaaatct ctcaacgaaa gccgtcctga
    20401 aaatgaaaaa gtttgggtta tcggtgttga ccgtgaccaa gaagcagaag gtaaatacac
    20461 ttctaaagat ggcaaagaat caaactttgt tcttgtatct actttgaaac aagttggtac
    20521 aactgtaaaa gatatttcta acaaggcaga aaaaggagaa ttccctggcg gtcaagtgat
    20581 cgtttactca ttgaaggata aaggggttga cttggcagta acaaaccttt cagaagaagg
    20641 taaaaaagct gtcgaagatg caaaagctaa aatccttgat ggaagcgtaa aagttcctga
    20701 aaaataatgg atggaagtca tttcttagga agcggccaag ggccgctttt ttaagagttg
    20761 agacaaagtc attttgtctc agtaaagctt gctactagac aaactagcaa gttttattga
    20821 aataaaataa gactctgaaa ggaagagcac atggcacacg aaaatgtcat tgagatgcgt
    20881 gatattacca aggtgtttgg tggatttgtt gctaacgaca aaatcaactt gcacctacga
    20941 aaaggtgaaa ttcatgcact tttaggagaa aatggggctg gtaagtccac gctaatgaac
    21001 atgttagcag ggcttcttga accaactagt ggtgaaatcg cggtcaacgg tcaagttgtc
    21061 aatctcgact ccccatctaa agcagctagc ttgggaatcg ggatggttca ccagcacttt
    21121 atgttggttg aagccttcac agtggctgaa aacatcattt taggtagtga attgactaaa
    21181 aatggtgtgc tagatatcgc tggagctagc aaagaaatca aggctctttc tgaacgttat
    21241 ggcttagctg ttgacccttc tgccaaggta gcagatatct cagttggagc ccaacaacgt
    21301 gtagaaattt taaaaacact ttatcggggg gctgatatcc ttatctttga cgaaccaacg
    21361 gctgttttga ctccatcaga aattgatgag ttgatggcta ttatgaaaaa tcttgtcaaa
    21421 gaaggaaaat caattatctt gattacccac aaattggatg aaattcgagc agtttctgac
    21481 cgtgttacag ttatccgtcg tgggaaatca attgaaaccg ttgaaattgc aggggctacc
    21541 aatgctgatt tggcggaaat gatggtagga cgttctgttt cctttaaaac agagaagcaa
    21601 gcctctaaac caaaagaagt ggttttgtct atcaaagatt tggtggtcaa tgaaaaccgt
    21661 ggtgttccag ctgttaaaaa tctgtccttg gatgttcgtg ctggagagat tgttggtatt
    21721 gcggggattg atggaaatgg tcagtctgaa ctgattcaag ccattacagg tcttcgtaag
    21781 gttgaatctg gtagcattga gctaaaagga gattcaattg taggcttgca cccacgtcag
    21841 attacagaac taagtgttgg gcacgttcca gaagaccgtc accgtgatgg cttgattttg
    21901 gaaatgatga tatctgaaag tattgccctt caaacctact ataaagaacc acatagtaaa
    21961 aatggaattt tgaattattc aaatattact tcttatgcta aaaagctgat ggaagagttt
    22021 gatgttcgcg ctgccagtga attagttcct gcagctgcac tctcaggagg aaatcaacaa
    22081 aaagcaatta ttgctcgtga aattgatcga gatcctgatc tccttatcgt tagccagcca
    22141 actcgtgggt tggatgtcgg tgccattgag tatatccaca aacgcttgat tgaagagcgt
    22201 gataatggca aggctgtcct tgttgtcagc tttgaattgg atgagatttt aaacgtctca
    22261 gaccgtattg ccgttatcca cgatggtaag attcaaggta ttgtatcacc agaaacaacc
    22321 aataaacaag aacttggtgt cttgatggct ggtggaaact tgggaaagga gaagagtgat
    22381 gtctaaaaaa ttacaacaaa tttcggttcc cttgatttct gtatttctag gaattttact
    22441 cggagccatt gtcatgtgga tcttcggtta tgatgctatt tggggctacg aagaattgtt
    22501 ctatacagcc tttggcagtc tgcgtgggat tggagaaatc ttccgtgcta tgggtcctct
    22561 ggtcttgatt ggtcttggtt ttgcggttgc cagtcgagct ggtttcttta acgtcggact
    22621 tcctggtcag gctttggcag gttggattct cagtggttgg tttgcgctgt cgcatccaga
    22681 tatgccccgt cccttgatga ttctagcaac catcgtgatt gccttgattg ctggtgggat
    22741 tgtcggagcg attccaggta ttcttagggc ctatctaggg acgtcagagg ttattgtaac
    22801 catcatgatg aactacattg tcttgtatgt agggaatgcc tttatccatg ctttccctaa
    22861 agacttcatg caaagtacag attcgaccat tcgtgttggg gctaatgcaa cctatcagac
    22921 accttggttg gctgagttga ctggtaactc acggatgaat attggtattt tctttgccat
    22981 cattgccgtt gcagttattt ggttcatgct caagaaaaca actcttggtt ttgaaatccg
    23041 tgcagttggt cttaatccac atgcttcaga atatgctggt atttctgcca agcggactat
    23101 tatcctatct atgattattt caggtgcctt ggcaggtctt ggtggagctg ttgaaggttt
    23161 gggaaccttc cagaacgtct atgttcaagg ttcgtcatta gctatcggat ttaacggaat
    23221 ggcggttagt ttgcttgcgg ccaactcacc aattggtata ctctttgcag ccttcctatt
    23281 tggcgttctc caagttgggg ctcctggtat gaatgcggcg caggtaccat ctgagcttgt
    23341 cagcattgta acagcgtcta ttatcttctt tgtcagtgtt cattacctta tcgaacgctt
    23401 tgtcaaaccg aaaaaacaag ttaaaggagg taagtaaaga tgtctattat aaccttgctc
    23461 cccctcttgg tgtcttctat gctgatttac tcagcacctc tcatctttac aagtatcggt
    23521 ggtgttttct ctgaacgtgg tggtgtggta aacgttggcc ttgaaggaat tatggttatg
    23581 ggtgcctttt ctggagttgt ctttaacctt gaatttgcag aacaatttgg agcagcaact
    23641 ccatggctat ccttgcttgt agcaggattg gttggtagtg ttttctctat catccacgca
    23701 gcagcgacgg ttcatttccg tgcagaccat gttgtcagcg gtacggtatt gaacttgatg
    23761 gcgcctgcct tggctgtttt cttagttaaa gttctttata acaaaggaca aaccgacaac
    23821 ctaagtcaaa cttttggacg ctttgatttc ccagtcttgg caaatatccc agtgattggt
    23881 gatatcttct tcaagtcaac tagtctactt ggttatctgg cgattgcctt ctcattcctt
    23941 gcttggttta ttctctttaa gactcaattt ggtcttcgtc tccgctctgt cggtgaacat
    24001 cctcaagcag cggatacctt gggaatcaac gtctacaaga tgagatattt aggggttatt
    24061 atttcaggtt ttctaggtgg aattggcgga gcgatttatg cgcaatcaat ctcagttaac
    24121 ttctcagtga caactattgt tggacctgga tttatcgccc ttgctgcgat gatctttggg
    24181 aaatggaatc caatcggtgc tatgctttct agtctcttct ttggactttc acaaagtttg
    24241 gctgttatcg gttctcaatt gccgttccta caaggagtgc cagcggttta tcttcaaatt
    24301 gcaccttatg ttttgacaat tcttgtctta gcagccttct ttggaaaagc agtcgcccca
    24361 aaagcagatg gtatcaacta catcaaatca aaataagcat acaaaaaaac gtcagtccaa
    24421 tgactgaact accccccaaa agttagacag aaaaaatcta acttttgggg tgtttttatt
    24481 atgaaactaa cttatgatga taaagctcag atctatgaac ttagaaaaca aggatatagc
    24541 ttagagaagc tttcaaataa atttgggata aacaattcta atattaggta catgattaaa
    24601 ttgattgatc gttacggaat agagttcgtc aaaaaaggaa aaaatcgtta ctattttcct
    24661 gatttaaaac aagaaatgat taataaagtc ttacatgaag gctgaactaa agatagagtt
    24721 tctcttgaat acggcctccc aagtcgtacg atacttctta attggctagc acaatacaag
    24781 aaaaacgggt atactattgt tgagaaaaca agagggagag tacctaaaat gggacgtaag
    24841 ccacaaaaga gacctgaaga gaggacagaa ttagaacgtc ttcaatcaga aaatgagtac
    24901 ctgagagcgg agaatgccat cctaaaaaag ttaagagaac tccgattgaa ggaggaaaaa
    24961 gagaaagaag aaagacagaa attgttcaag aattaatgac tgagttttcg ttagttcttc
    25021 ttctaaaatc cattaaacta gctcgttgga cctactacta tcacttgaaa cagctagaca
    25081 aaacagataa ggaccaagag cttaaagctg aaattcaatc catttttatc gaacacaaag
    25141 gaaattatgc ttatcgccgg gttcatttag aactaagaaa tcgtgcttat ctggtaaatc
    25201 ataaaagagt tcaaggcttg ataaaagtac tcaatttaca agctaaaatg cgacagaaac
    25261 gaaaatattc ttctcataaa ggagacgttg gcaagaaggc agagaatctc attcaaggtc
    25321 aatttgaagg ctctaaaaca atggaaaagt gctacacaga tgtgactgaa tttgccattc
    25381 cagcaagtac tcaaaagctt tacttatcac cagttttaga tggctttaac agcgaaatta
    25441 ttgcttttaa tctttcaact tcgcccaact tagaacaagt gaagagtatg ttagaacagg
    25501 cctttacaga gaaatactat gagaatacga ttctccatag tgaccaaggc tggcaatacc
    25561 gacacgattc ttatcatcgg ttcctagaga gtaagggagt tcaagcatcc atgtcacgta
    25621 agggtaacag cccagacaat ggtatgatgg agtccttctt tgggattctg aaatcggaaa
    25681 tgttttatgg ctatgagaaa acatttaaat cacttaatca attggaacaa gctattgtag
    25741 actatattga ttactacaac aataaacgaa ttaaggtaaa actaaaagga cttagccttg
    25801 tgcaatacag aactaaatcc ttcggataaa ttaggctcta taatatttgt agtgggtaaa
    25861 tcccctatag atattatgga gcctattttt gtgtagaaaa aaagtcccat atgacctata
    25921 atgaaaagcg acaaaacaac tcattagaaa gattcatatg gaacaattac attttatcac
    25981 aaaattacta gacattaaag acccaaacat caagattcta gatatcatca ataaggatac
    26041 acacaaagaa atcatcgcca aactggacta cgacgcccca tcttgccctg agtgcggaag
    26101 tcaaatgaag aaatatgact ttcaaaaacc gtctaagatt ccttatcttg aaacgactgg
    26161 tatgcctact agaattctcc ttagaaagcg ttgattcaag tgctatcact gttcaaaaat
    26221 gatggtcgct gaaacttcta tcgtcaagaa gaatcaccaa atccctcgta tcatcaacca
    26281 aaaaattgct caaaagttaa ttgaaaagat ttctatgact gatattgccc atcagctttc
    26341 cacctcaact tcaactgtta ttcgtaagct caatgatttt cactttaaac atgatttttc
    26401 ttgtcttcct gagattatgt cttgggatga gtatgctttt acaaagggaa agatgagttt
    26461 cattgcgcaa gattttgata atcttaatat tatcactgtt cttgaaggca gaacacaagc
    26521 tgtcatccga aatcactttc ttcgctacga tagagccgtt cgttgtcaag tgaaaatcat
    26581 tacgatggat atgtttagtc cttactatga cttggctaaa cagcttcgct ttcaaatttc
    26641 taggctcagg ctgaaacagt ctcccagact gtttcactcc cgaatgctaa aatcgttctt
    26701 gatcgctttc acattgtaca acatcttagc cgtgctatga gtcgtgtgcg tgtccaaatc
    26761 atgaatcagt ttcatcgaaa atcccatgaa tacaaggcta tcaagcgcta ctggaaactc
    26821 attcaacagg atagccgtaa actgagtgat aaacgttttt atcgtcctac ttttcgtatg
    26881 cacttaacca ataaagagat tttaaacaag cttttgagtt attcggaaga cttgaaacac
    26941 cactatcaac tctatcaact cttgctgttt cacttccaga ataaggaacc agagaaattt
    27001 ttcgaactta tcgaggacaa tcttaagcag gttcatccta tttttcagac tgtctttaaa
    27061 accttcctca aagataaaga aaagattgtc aacgcccttc aactacacta ttctaatgcc
    27121 aaactggaag cgaccaataa tctcatcaaa cttatcaagc gcaatgcctt tggttttcga
    27181 aactttgaaa acttcaaaaa acggattttc atcgctctga atatcaaaaa agaaaggaca
    27241 aaatttgtcc tttctcgagc ttagctgact tcaacccact acagttgaca aagagccata
    27301 aattatttgt ctaactgttt gggggcagta cagaactggc gttttatttt ttaggatctt
    27361 gatgggttag gttcaatccc caagggacca aattttcagt tttatttttg atacgagcta
    27421 tattgtcctt atgacgaatg ataatcaaac tagcaagtgc taagataata gcgatgaaga
    27481 gagagtcata gttactcagg ataaaaccaa aaagtggaaa gagcagaacc ccgataacag
    27541 ccgcgatcga tgctgtgaca ctagacagtg aaatcatact gccaagatag agagctccaa
    27601 agaagataat cgcaaggtag agacagaaga taggcgcaaa tccgaaaatc actccagcac
    27661 tggttgcgac agccttacca cctttaaatc ctgcaaagat agggaaggta tggccgataa
    27721 cagccaaaag tccaaagatg agaggagaaa cgccttgtag atgaaaaata atcggaagca
    27781 gcgttgctag ggttcctttg aaaaagtcaa tcacaaaggt tgccatacca gctttcttac
    27841 ctaaaatgcg gaaggtgttg gtcgttccag tgttaccaga accatgctcg cgtagattga
    27901 tttgaaagaa tacttgtcca atccagagac cagatggaat cgaacccagc agataggcta
    27961 ggattaataa aactattgta atcatactcc tattatatca tgaattggga aagaaaagca
    28021 gagaatctct gctgaaattg tcacatcgga gaaagaaaaa ttttgcaaaa tccttggaaa
    28081 acctgtagaa tagtaaagat gaacgaatag gaggttcctt gtgtcaaaaa aggaaatcaa
    28141 tattaacaat tataatgatg atgctattca ggtgctagaa gggttggatg cggtccgaaa
    28201 acgtccaggg atgtatattg gatcgaccga tggcgctggt cttcatcacc tagtttggga
    28261 aatcgttgat aatgcagtcg atgaagcctt gtctgggttt ggtgatcgta ttgatgtaac
    28321 tatcaataaa gacggtagtc taacggttca agaccatgga cgtgggatgc cgacaggtat
    28381 gcacgctatg ggaattccaa ctgttgaggt tatctttacc attcttcatg ccggagggaa
    28441 attcggtcaa ggtggctata agacatcagg tggacttcac ggagtgggtt cttccgttgt
    28501 taacgccctt tctagctggt tagaagttga aattacccgt gatggcgcag tttacaagca
    28561 acgttttgaa aatggtggaa aacctgtcac gactttgaag aaaatcggta cagcacccaa
    28621 gtctaaaaca ggcaccaaag ttacttttat gcctgacgcg actatctttt ctacgacaga
    28681 tttcaagtac aataccattt cagagcgcct taatgaatca gcctttctct tgaaaaatgt
    28741 gaccttgtct ttaacggaca agcgaacaga tgaagcgatt gagttccact atgagaatgg
    28801 agtacaagat tttgtttctt atctcaacga agataaggaa atcttgacgc cagttcttta
    28861 ctttgaaggg gaagacaatg gttttcaagt ggaagtagcc ctccagtaca atgacggatt
    28921 ctcagataac attctatcct ttgtcaataa cgttcgcacc aaggacggtg gaacgcacga
    28981 gacaggactc aagtctgcca ttaccaaggt catgaatgac tatgcacgta aaacaggtct
    29041 tctcaaggaa aaagataaaa accttgaagg ttcagactat cgtgagggac tagcggccgt
    29101 tctttctatc ttagttcctg aagaacactt gcagtttgaa ggacagacca aggataaact
    29161 aggaagcccc ctagctcgcc cagttgtgga tggaatagtg gctgataagt tgaccttttt
    29221 ccttatggaa aatggggaat tagcttctaa cctcatccgc aaggctatca aggcccgtga
    29281 tgctcgtgaa gcagcacgta aggcgcgtga tgagagccga aatgggaaga aaaacaagaa
    29341 agataagggc ttgttgtctg ggaaattgac cccagcccaa tctaagaatc ctgctaagaa
    29401 tgaactctat ctagttgagg gggactctgc cggtggttct gccaaacaag gtcgtgaccg
    29461 caagttccag gctattctac ctcttcgtgg taaggttgtc aatacagcca aggccaagat
    29521 ggcggatatc ctcaaaaatg aagagatcaa taccatgatt tataccattg gtgcgggtgt
    29581 tggagcagac ttctctattg aagatgccaa ctatgataag atcattatca tgaccgatgc
    29641 ggataccgac ggtgcccata tccagacctt gctcttgaca tttttctacc gttacatgcg
    29701 tccgctagtc gaggcaggtc atgtctatat tgccctccca cctctttata agatgtccaa
    29761 aggtaaaggc aagaaagaag aagtggccta cgcttggacg gacggagaac tagaagaact
    29821 ccgtaaacag ttcggtaaag gcgctaccct ccaacgatac aaaggacttg gtgagatgaa
    29881 tgcggaccag ctctgggaaa caaccatgaa cccagaaaca cgtaccctca tccgtgtcac
    29941 aattgaagat ttagcgcgcg ccgaacgccg cgtcaatgtt ctcatgggag ataaggtaga
    30001 accacgccgt aaatggattg aagataatgt caagtttacg ctagaagaag cgacagtgtt
    30061 ttaatgaaag aaaataatat gttagtaaac tggaaattag agtcggatgt gaatgactat
    30121 gtgaaaaagc agtttgaaaa tcttggtttg aaaaaattgc aggattatta atgaagaatc
    30181 tgctatgagt gcttatctca aagaggcgct taagggagcg gctaagacac aaactaagac
    30241 taattttgga aaaccagatt ttcatattga aaaatacaaa caggatagtt tggttactta
    30301 tcgtaactga acacgggagg aattcccgta aaatatcttt tactgcataa aaacctactc
    30361 tacattcttt gaaaggagtt gaacacgccc tagatactgt gtgaaaaaga taaattctct
    30421 tgtgagtttg cttacttcaa gaattttcta ttttcacttg gtattttatg ggctttgtat
    30481 cttatgtcta acattcaaaa catgtccctg gaggacatca tgggagagcg ctttggtcgc
    30541 tactccaagt acattattca agaccgggct ttgccagata ttcgtgatgg gttgaagccg
    30601 gttcagcgcc gtattcttta ttctatgaat aaggatagca atacttttga caagagctac
    30661 cgtaagtcgg ccaagtcagt cgggaacatc atggggaatt tccacccaca cggggattct
    30721 tctatctatg atgccatggt tcgtatgtca cagaactgga aaaatcgtga gattctagtt
    30781 gaaatgcacg gtaataacgg ttctatggac ggagatcctc ctgcggctat gcgttatact
    30841 gaggcacgtt tgtctgaaat tgcaggctac cttcttcagg atatcgagaa aaagacagtt
    30901 ccttttgcat ggaactttga cgatacggag aaagaaccaa cggtcttgcc agcagccttt
    30961 ccaaacctct tggtcaatgg ttcgactggg atttcggctg gttatgccac agacattcct
    31021 ccccataatt tagctgaggt catagatgct gcagtttaca tgattgacca cccaactgca
    31081 aagattgata aactcatgga attcttgcct ggaccagact tccctacagg ggctattatt
    31141 cagggtcgtg atgaaatcaa gaaagcttat gagactggga aagggcgcgt ggttgttcgt
    31201 tccaagactg aaattgaaaa gctaaaaggt ggtaaggaac aaatcgttat tactgagatt
    31261 ccttatgaaa tcaataaggc caatctagtc aagaaaatcg atgatgttcg tgttaataac
    31321 aaggtagctg ggattgctga ggttcgtgat gagtctgacc gtgatggtct tcgtatcgct
    31381 atcgaactta agaaagacgc taatactgag cttgttctca actacttatt taagtacacc
    31441 gacctacaaa tcaactacaa ctttaatatg gtggcgattg acaatttcac acctcgtcag
    31501 gttgggattg ttccaatcct gtctagctac atcgctcacc gtcgagaagt gattttggcg
    31561 cgttcacgct ttgacaaaga aaaggctgag aaacgtctcc atatcgtcga aggtttgatt
    31621 cgtgtgattt cgattttgga tgaagtcatt gctcttatcc gtgcttctga gaataaggcg
    31681 gacgccaagg aaaacctcaa agttagctat gattttacgg aagaacaggc tgaggctatc
    31741 gtaactttgc aactgtaccg tttgaccaat accgatgtgg ttgtcttgca ggaagaagaa
    31801 gcagagcttc gtgagaagat tgctatgctg gcggctatta tcggtgatga aaggactatg
    31861 tacaatctca tgaagaaaga acttcgtgag gtcaagaaga aatttgcaac tcctcgtttg
    31921 agttctttag aagacactgc gaaagcaatt gagattgata cagctagtct tatcgctgag
    31981 gaagatacct acgtcagcgt gaccaaggca ggttacatca agcgtaccag tccacgttcc
    32041 tttgcggctt ccaccttgga agaaattggc aagcgtgatg atgaccgttt gatttttgtt
    32101 caatctgcca agacaaccca gcacctcttg atgttcacaa gtcttggaaa tgtcatctac
    32161 agaccaatcc atgagttggc agatattcgt tggaaggaca tcggagagca tctgagccaa
    32221 accatcacaa actttgaaac gaatgaagaa atcctttatg tggaagtact ggatcagttt
    32281 gacgatgcga caacctactt tgcagtgact cgccttggtc aaatcaaacg ggtagagcga
    32341 aaagaattca ctccatggcg gacctataga tctaagtctg tcaagtatgc taagctcaaa
    32401 gacgatacag atcagattgt agcagtggct ccgattaaac tagatgatgt tgtcttggtt
    32461 agtcaaaatg gttatgccct gcgtttcaat atcgaagagg ttccggttgt cggtgctaag
    32521 gcagcaggtg tcaaggctat gaatttgaaa gaagatgatg tcctccaatc tggctttatc
    32581 tgtaatactt cgtccttcta cctcttgacc cagcgtggaa gcttgaaacg tgtttccatt
    32641 gaggaaattc tagcaaccag ccgtgccaaa cgaggattac aagtcttgcg tgagttgaaa
    32701 aacaaaccgc atcgtgtctt cttggcagga gcagttgcag agcaaggatt tgttggcgat
    32761 ttcttcagta cggaagtgga tgtgaacgac caaactctgc ttgtccaatc caataaagga
    32821 acaatctatg aaagccgatt gcaagacttg aacttgtcag aacgcactag caatggaagc
    32881 ttcatttctg acacgatttc agatgaagaa gtttttgacg cttatcttca ggaagtagtt
    32941 actgaagata aataagtgaa atgaagaatc agttctaaga gctggttttt attggagaaa
    33001 ttttctgaaa attacaaaat atacttgcta ttttagaaaa atagtgtaga atataagaaa
    33061 taggttttta gaataaggag tggaatatga cagtaacgat tgattgggaa aacctcggtt
    33121 tttcctatat gaaattacct tatcgctatc ttgctcattt caaaaatgga caatgggatc
    33181 aaggagagct tacagaggat gcaactttgc atatttcaga gtcttctcca agtcttcact
    33241 atggacaaca agcatttgaa ggtttgaaag cttatcgtac taaggatggc agtgttcaac
    33301 tgttccgtcc tgatgaaaat gctaaacgcc tgcaacgtac atgtgaccgt ctcttgatgc
    33361 cacaagttcc gacagacatg tttgtagaag cttgtaaagc agttgtccgt gcgaatgaag
    33421 aatacgtacc accatacgga ataggtggaa ccttatatct tcgccctctt ttgattggtg
    33481 tcggagatat tatcggggta aaaccggcag aagaatacat tttcaccatc tttgctatgc
    33541 cagttggaaa ttactttaaa ggtggtttgg tcccaaccaa cttcttgatt caggatgagt
    33601 acgaccgtgc agcaccaaat ggtacaggtg cggctaaggt tggtggaaac tatgctgcaa
    33661 gtctcttacc aggaaaaatg gccaagtcac gtcatttctc agatgttatc tatctggacc
    33721 catcaactca tacaaagatt gaagaagtcg gatcagctaa cttctttgga attacagctg
    33781 ataatgaatt tgtaacacca ttgagtccat ctatcttgcc atctattacc aagtattcct
    33841 tgctttattt ggcagaacat cgcttgggat taactcctat tgagggtgat gttccaattg
    33901 ataatcttga ccgttttgta gaggcaggtg cctgtggtac agcagcggtt atttctccaa
    33961 ttggaggtat tcaacatggt gatgatttcc atgtattcta tagtgaaaca gaagtaggtc
    34021 ctgtgacgcg taaattatat aatgaattga cgggtattca gtttggcgat attgaagcgc
    34081 cagaaggttg gattgtaaaa gtagattaaa ataaaccaaa ggagattttt tatgaaatag
    34141 aaaaagtggc tcttaacagc aggagtggtc ctgagcacgt cagctatttt agtggcttgt
    34201 ggaaaaactg ataaagaagc agatgcaccg acaacatttt cttatgtcta tgcagtagat
    34261 ccagcatcat tgggctacag tatagcgact cgaacatcga cgacagacgt tattggaaat
    34321 gttattgatg gtttgatgga aaatgataaa tacggcaatg ttgctccttc tcaaaaagac
    34381 tatgatttga atagtacagg atgggctcca agctatcaag atccagcgtc ttacttgaat
    34441 attatagatc caaaatctgg ttctgccatg aaacaccttg gtattacgaa aggaaaagat
    34501 aaggatgttg tagctaaacc tggtttggat aaatataaga aattgttaga agatgctgtt
    34561 tctgagacca ctgacctaga gaaaagatat gaaaaatatg ccaaagctca agcttggtcg
    34621 acagatagtt cattattgat gccaacagct tcatctggtg gttctccagt tgtaagtaac
    34681 gtagtaccat tctcaaaacc atactcacaa gttggtatta agggggatcc atatatcttt
    34741 aaaggaatga aattgcaaaa agatattctt acaacaaaag aatataacga ggtttttaaa
    34801 aaatggcaaa aagaaaaatt ggaatccaat agcaaatacc aaaaagaact agaaaaacac
    34861 attaaataag aaatggtatt gatcttgata aaattttaaa aatactgtca ttttgaatat
    34921 aaaggagttt gatatggagt ggattagatt aataggaata gcaatcattg ttgtgggttt
    34981 tattttaaaa tttgatacaa ttgcaacagt agtcttagct ggtttggtta cagctttagt
    35041 ttcaggtgtt tctctcgttg aatttttgga gattttggga aaagaattta gcaatcagcg
    35101 agtgctcacg atttttatgg ttaccttgcc tcttgtgggg ctgtcagaaa cctttggact
    35161 caagcaacga tcaatcgatt tgattcgaaa gattaaaggt ctgacagttg gaaacttcta
    35221 tacagtttat ttctttattc gagagttagc tggtttcttt tcaattcgtc taggaggaca
    35281 ccctcagttt gtcagacctt tggttcaacc tatgggagaa gcagctgcag agtctcaatt
    35341 aggtagaaag ttaacagagg ttgaagatga gacaataaaa gcgcgtgcgg ctgcgaatga
    35401 aaattttgga aatttctttg ctcaaaatac gtttgtaggt gctgggggag tcctcttgat
    35461 agggggaaca ttagatcagt taggctatga aagcaattat gcagggattg cttctacatc
    35521 tattattgtt gctgttataa cacttattgt agtggggatt tacaattatt tatttgataa
    35581 aaaattgata tcaaaaaaga ctaggggagg agaacaaaaa tgacagagtt ggcaaagcaa
    35641 ctattagagt tgacctatat tgtgattggt tgtcaatttc tccatacagc ctattgtagt
    35701 tataaagata aaacaaaccc agttcgactt gggacatctg cattttggac tctattgtct
    35761 attacgttta taggtggttc ctatatgcca aatatgagta ttggtattat tgtaatccta
    35821 ttatcgctgt taacattgtt taagcaagtc cgtatcggaa ccttgccatc cttagatgaa
    35881 atgaaagcca atattgaatc taacaggttg aaaaataaaa tttttattcc agttatgctg
    35941 atggcaatac ttgcgttggt cttagcgcaa atgattccag aatttagcaa gatttcgatt
    36001 agccttgccg ccttgtttgc tacaatttct gttcttgtga ttaccaatag tcaccctaag
    36061 agtctgttat cagaaaataa tcgaatgact cagcaagttt caacaagtgg gattgttcct
    36121 caattattag gggctttggg ggctattttt actgtagcag gtgttggtga tgttatctct
    36181 catctgatta gcggtattgt tccttcagat agtcgcttta taggagtttt ggcctatgtt
    36241 cttggaatgg ttctattcac aatgattatg ggaaatgctt ttgcagcatt caccgttatt
    36301 acagcaggtg ttggagttcc ctttgtattt gctctgggag ctaatccaat tgtggctggt
    36361 gctcttgcca tgacagcagg ttattgtggg accttattga ccccaatggc tgctaatttt
    36421 aacgctctac cagcagcatt gatggatatg aaagatcaga atggcgttat aaaggctcaa
    36481 gcaggtgttg ctctagtaat gattgttatt cacatattct taatgtactt tctcgcattt
    36541 tagtaaagga aaataaaatg aaaatattgg ttacaggttt taatcctttt ggaggtgaaa
    36601 agattaatcc agctttggag gctgtaaaat tattaccatc tgagattaat ggggctgaag
    36661 ttcgctgggt agaaattcca acggtttttt ataagtcgtc agaagtttta gaggcagaaa
    36721 tattacgata tcaaccagat gctgtacttt gtattggaca agcaggcggc aggaccggct
    36781 taacacctga acgagtggct attaatcaag atgatgctcg catacctgat aacgaaggca
    36841 atcaaccaat tgatacaccg attcgtattg atggagcatc ggcctatttt agtagtttac
    36901 ctatcaaagc gatggtacaa gctatcaaaa aacagggact tccggcagtt gtatccaata
    36961 gtgcaggaac ctttgtttgc aatcatttga tgtaccaagc tctttattta gtggataaaa
    37021 aattccctaa tatgagagca ggtttcatgc atattccata tatgatggaa caagtagtaa
    37081 ataagccgaa tactgcagga atgagtctat gtgatattgt aagaggcata gaggtagcta
    37141 ttgaagctat tgtagactac aaagataagg atttgcagtt agtaggcggt gagactcact
    37201 gataacctaa aaaggatagt caattatgct tgtttactaa ctattagcta tgctaaatcg
    37261 attgaggttg tttacataaa actctatatc agagaagcct gatatagagt tttttcttgc
    37321 tagttttagg attttcttgt aaaatagaaa aagtgaagag aggtatgaaa tgagcaagaa
    37381 agataaaaaa atcgaaattc aagtagcgga tgccaaagtt aatgttggta aagacagttt
    37441 tgaaggttat acattgacta tcggtaaaaa agttatcgga gaaattgccg aattagacgg
    37501 acaatttgcc attataaaga atgggaatgt cgatagtttt tataaaaaat tggaaaaagc
    37561 tgtggaaatt ttgattgaaa attataattt agcaaaataa gtcttgtttt tttgaaattt
    37621 tcatgatata atagtccata ttgattgtag gagagatagc gaagaggcta aacgcggcgg
    37681 actgtaaatc cgctccttcg ggttcggggg ttcgaatccc tctctctcca tttcattaat
    37741 ggggtatagc caagcggtaa ggcaagggac tttgactccc tcatgcgttg gttcgaatcc
    37801 agctacccca gttcttaggt aataatcaag ataaaaagca aaatatctta gggtatttta
    37861 tttttataat tgaaagacgt gaatgatatg aacatgtcct tgcgggtgct taggaaaaaa
    37921 attataagta tgtcaagttt aagaaaaact tgattgttgg aggatttttt agatgaacga
    37981 atttgaagat ttgctaaata gcgttagtca agttgagact ggtgatgttg ttagtgctga
    38041 agtattgaca gttgatgcga ctcaagctaa cgttgcaatc tctggaactg gtgttgaagg
    38101 tgtcttgact cttcgcgaat tgacaaacga tcgtgatgca gatatcaatg actttgttaa
    38161 agtaggagaa gtattggatg ttcttgtact tcgtcaagta gttggtaaag atactgatac
    38221 agttacatac cttgtatcta aaaaacgcct tgaagctcgc aaagcatggg acaaacttgt
    38281 tggtcgcgaa gaagaagttg ttactgttaa aggaacgcgt gccgttaaag gtggactttc
    38341 agtagaattt gaaggtgttc gtggatttat cccagcttca atgttggata ctcgtttcgt
    38401 acgtaacgct gagcgttttg taggtcaaga atttgatact aaaatcaaag aagttaacgc
    38461 taaagaaaac cgcttcatcc tttcacgtcg tgaagttgtt gaagcagcta ctgcagcagc
    38521 tcgcgctgaa gtattcggta aattggctgt tggtgatgtt gtaactggta aagttgctcg
    38581 tatcacaagc ttcggcgctt tcatcgacct tggtggtgtt gacggattgg ttcacttgac
    38641 tgaattgtca catgaacgta acgtatcacc aaaatcagtt gtaactgttg gtgaagaaat
    38701 tgaagtgaaa atccttgatc ttaacgaaga agaaggacgt gtatcacttt cacttaaagc
    38761 aacagtacca ggaccatggg atggcgttga gcaaaaattg gctaaaggtg atgtagtaga
    38821 aggaacagtt aaacgtttga ctgacttcgg tgcatttgtt gaagtattgc caggtatcga
    38881 tggacttgtt cacgtatcac aaatttcaca caaacggatt gaaaatccaa aagaagctct
    38941 taaagttggt caagaagttc aagttaaagt tcttgaagtt aacgcagatg cagaacgcgt
    39001 gtcactttct attaaagctc ttgaagaacg tccagcccaa gaagaaggac aaaaagaaga
    39061 aaaacgtgct gctcgtccac gtcgtccaag acgtcaagaa aagcgtgatt tcgaacttcc
    39121 agaaacacaa acaggatttt caatggctga tttgtttggt gatatcgaac tttaatcaaa
    39181 ttgaaaattc acaaaatcct ttgtttacta aacaaggaat tttattggct ctttgtcaac
    39241 tgtagtgggt tgaagaa
//
LOCUS       DAVKWZ010000012        37617 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000012 DAVKWZ010000000
VERSION     DAVKWZ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37617
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.012"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(117..599)
                     /gene="greA"
                     /locus_tag="VW854_000696"
     CDS             complement(117..599)
                     /gene="greA"
                     /locus_tag="VW854_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEV0261973.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            complement(670..2325)
                     /gene="mltG"
                     /locus_tag="VW854_000697"
     CDS             complement(670..2325)
                     /gene="mltG"
                     /locus_tag="VW854_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEV0261974.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPATPTPATETVDI
                     ISDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQAVGQLQGDFKESLTAETF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            complement(2406..2900)
                     /locus_tag="VW854_000698"
     CDS             complement(2406..2900)
                     /locus_tag="VW854_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0261975.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVDLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            complement(2897..3361)
                     /locus_tag="VW854_000699"
     CDS             complement(2897..3361)
                     /locus_tag="VW854_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0261976.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQCLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKVIRLESPDELLSYFGMNGFVDEEETLLYATSQGYSMIWFNPFYLEEQ"
     gene            complement(3371..4705)
                     /locus_tag="VW854_000700"
     CDS             complement(3371..4705)
                     /locus_tag="VW854_000700"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEV0261977.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGSDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFAGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            complement(4716..5333)
                     /locus_tag="VW854_000701"
     CDS             complement(4716..5333)
                     /locus_tag="VW854_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV0261978.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPITEEKPVKPIEKQVEKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSTPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            complement(5381..8479)
                     /locus_tag="VW854_000702"
     CDS             complement(5381..8479)
                     /locus_tag="VW854_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV0261979.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVSDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQDIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPRQATQVQADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            complement(8572..9738)
                     /locus_tag="VW854_000703"
     CDS             complement(8572..9738)
                     /locus_tag="VW854_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV0261980.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVLLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDSLFEIDFDQLEKDLVEEE
                     VKLYVLCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKR"
     gene            complement(9746..10840)
                     /locus_tag="VW854_000704"
     CDS             complement(9746..10840)
                     /locus_tag="VW854_000704"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV0261981.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGRFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQTLEG"
     gene            complement(10997..11720)
                     /locus_tag="VW854_000705"
                     /pseudo
     CDS             complement(10997..11720)
                     /locus_tag="VW854_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(11745..12680)
                     /locus_tag="VW854_000706"
                     /pseudo
     CDS             complement(11745..12680)
                     /locus_tag="VW854_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(12852..14810)
                     /locus_tag="VW854_000707"
     CDS             complement(12852..14810)
                     /locus_tag="VW854_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV0261982.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKEVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGIESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            complement(15013..16635)
                     /locus_tag="VW854_000708"
     CDS             complement(15013..16635)
                     /locus_tag="VW854_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV0261983.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            16741..18186
                     /locus_tag="VW854_000709"
     CDS             16741..18186
                     /locus_tag="VW854_000709"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEV0261984.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVIFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVDIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTATYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQAYLVHRVYGLTFDVGVFLNITPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            complement(18232..18426)
                     /locus_tag="VW854_000710"
     CDS             complement(18232..18426)
                     /locus_tag="VW854_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV0261985.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTK"
     gene            complement(18527..19122)
                     /locus_tag="VW854_000711"
                     /pseudo
     CDS             complement(18527..19122)
                     /locus_tag="VW854_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
     gene            complement(19159..19671)
                     /locus_tag="VW854_000712"
     CDS             complement(19159..19671)
                     /locus_tag="VW854_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEV0261986.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERTCRHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            complement(19758..20693)
                     /locus_tag="VW854_000713"
     CDS             complement(19758..20693)
                     /locus_tag="VW854_000713"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEV0261987.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            complement(20764..21063)
                     /locus_tag="VW854_000714"
     CDS             complement(20764..21063)
                     /locus_tag="VW854_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEV0261988.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALFKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            complement(21053..21802)
                     /locus_tag="VW854_000715"
     CDS             complement(21053..21802)
                     /locus_tag="VW854_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEV0261989.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICHSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            complement(21856..22215)
                     /locus_tag="VW854_000716"
     CDS             complement(21856..22215)
                     /locus_tag="VW854_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEV0261990.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            complement(22217..23617)
                     /locus_tag="VW854_000717"
     CDS             complement(22217..23617)
                     /locus_tag="VW854_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEV0261991.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSYVEEYI
                     SWVKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDVIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            complement(23912..24151)
                     /locus_tag="VW854_000718"
     CDS             complement(23912..24151)
                     /locus_tag="VW854_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEV0261992.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            complement(24183..24653)
                     /gene="ssbA"
                     /locus_tag="VW854_000719"
     CDS             complement(24183..24653)
                     /gene="ssbA"
                     /locus_tag="VW854_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEV0261993.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            complement(24665..24955)
                     /locus_tag="VW854_000720"
     CDS             complement(24665..24955)
                     /locus_tag="VW854_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEV0261994.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            complement(25108..26454)
                     /gene="asnS"
                     /locus_tag="VW854_000721"
     CDS             complement(25108..26454)
                     /gene="asnS"
                     /locus_tag="VW854_000721"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEV0261995.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            complement(26470..26835)
                     /locus_tag="VW854_000722"
     CDS             complement(26470..26835)
                     /locus_tag="VW854_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV0261996.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLVPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGIEAGGRDNGVPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            complement(26828..28015)
                     /locus_tag="VW854_000723"
     CDS             complement(26828..28015)
                     /locus_tag="VW854_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV0261997.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAVVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYSFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTVILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            complement(28012..28500)
                     /locus_tag="VW854_000724"
     CDS             complement(28012..28500)
                     /locus_tag="VW854_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEV0261998.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISGSFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKITFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            28821..29369
                     /locus_tag="VW854_000725"
     CDS             28821..29369
                     /locus_tag="VW854_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV0261999.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKVEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIQ"
     gene            complement(29607..35087)
                     /locus_tag="VW854_000726"
     CDS             complement(29607..35087)
                     /locus_tag="VW854_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682791.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV0262000.1"
                     /translation="MKHSHKKSFDWYSMQQRYSIRKYYFGAVSVLLGTALVLGAAASV
                     QTVQAEENKQETTNSISVGRGEAATKPAEVSASNKEKTYAAPTVANPVETTPVKTGEV
                     TKPAEKVEEAKDKKEEVTHQDAIDKSKLLTALSRAKKLESKLYTEASAANLQTSIQAG
                     QSLLGKADASEAELSAAESSIQSSIIGLELRSNSNKGTVSETPVAKKANIVEAKEETN
                     PAATTDRSAVDSAVLPTSTAAKVETTSAPASTNEILKPGLSLSDARQNPAIRKEDVDR
                     GYSGFRTAGSGFRAAGPENKPILNPNNTIAFSDISQGSHSFRGIGHSRGGREIHYDVT
                     TVRRGNNINFTINYFAPGNLREFVNNNFILDKGTGYGEPTPATVITPNLRNQVKNISK
                     GGNFVSHSGYSLEAAVETNKSQTITFSLPITNPNGDLSLRLRPVEFNVDQGGSGTGTS
                     NDPYSNSNYYFETDPLYLDANPNQANPLTTSFNTKVDFQTLYIPTTKLAEGVTELVSE
                     GVEGEKSQAYRLHKFGDQYVLGLPVGNATTVTAAKPRVMKYGIGKIAATQNKTGDTNH
                     IKFYLDKDGDGVFNNQDEVILNTDIKDGERGPTGATGAQGPAGPKGADGQRGPAGPQG
                     PKGEQGNPGTPGKDGKSLIAVKNGTETKVYVEDPARPGQPLNPNQPLATITDGRNGTN
                     GQSPTITATRSVQNGKNGVLVTITPVEGRPQTTFVEDGQKGADGKTPTVTITEGQNGT
                     HTLTVHNPGSPDVTTTIRDGATGQAGRDGKDVLNGKVNPQPNQGKNGDKYINTETGDV
                     YVKNNGNWDKEGNIKGPKGDKGADGAKGEKGDQGERGLTGAQGAKGADGAVGRDGRDG
                     KDVLNGKANPEAHQGKDGDKYVNTETGDVFVKNNGNWDKEGNIKGPKGDKGADGAKGE
                     KGDRGERGLTGAQGAKGADGAAGRDGRDGRDGRDGKDVLNGKVNPEANQGKDGDKYVN
                     TETGDVFVKNNGNWDKEGNIKGSKGDKGERGEDGKTPEVTVTPGKDGHSTDITFTVPG
                     KDPVTVNVKDGENGLNGKTPKVDLLRVQGKNGNPSHTIVTFYTDENNDGKYTPGTDEL
                     LGSEMIKDGAKGADGRDGKSLLTVKDGKETKVYQEDPANPGQPLNPEKPLAVIRDGVD
                     GKSPTVTAVRKDEAGHKGVEITVDNHDGSQPTTVFVQDGAKGETGATGQDGQTPTITT
                     QRGQDGQSTVVTITTSGKDPVTFTVKDGKNGKDGRAPKIKVEDITSPSRIRRDTDAAA
                     TPTRNGIRVTVYDDVNDNGVYDEGVDKVLNSKDIYNGIDGRDGSAPTITTKDNGDGTH
                     TITVQNPDGSESTTVVKDGKDGKTANITTTENPDGSHTITVTNPDGSTKETVVKNGKD
                     GKTPKVEVTDNNDGTHTVKVTDGDGNVTNAIIKDGKDGKAATATTTENPDGSHTVTIT
                     NPDGTKNEFVVKNGRDGVDGRTPTASVRDNGDGSHTIVITNPEGVTTETTVRDGKSPK
                     VTITDEQNGTHKISVLNGDGTTTETIIKDGKSPVATVRDNQDGTYTIRVENGNGTVSE
                     TTVRDGKSLTAKVVDNGDGTHTITVVNSDGTTTTTTVRDGREPKLEVIDNNDGSHTIK
                     VTGADGKGTTTTIFDGKSPKANIVDNGDGTHTLTIVDSDGREYKSIIKDGKDGKDSVS
                     PTVTVKNNNDGTHVVTITNPDGSKTEMVIKDGKDGKCGCQDKPVPPTPNVPTPEVPVK
                     PVPAQPTPNVPTPEVPVQPTPAVSTPEVPVKPVPAVPEQPVVPTPAQPATPVNANPVA
                     PTTGKENRGDKLPETGSQSDYISVLLGSGILLSLYVGRRKED"
     gene            35303..36787
                     /locus_tag="VW854_000727"
     CDS             35303..36787
                     /locus_tag="VW854_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV0262001.1"
                     /translation="MRNLLSTKVQRQLRLMETLIQNRNWMKLHELAEKLGCTERILKS
                     DLNELRIAFPSINIQSSVNGIMIDLEVNTSVEDIYQYFLANSQSFQLLEYMFFNEGLP
                     IYRTIENLYFSSANLYRLGRNITKVLSSQFQIELSFTPSEIRGNEIDIRYFFAQYFSE
                     RYHFLDWPFPDLPEEDLTEFADFFYKITNYPMRFSIYRMYKLMIAISIHRVKNGHFID
                     LPNHFYKEYYPLLKSIPNFQETLAYFSKHFGLEMTPDTIAQIFISFLQNDIFLDPQEF
                     FNSLEDNSQARYSYQLLSQILERLSKQYKITFTNHDELIWHLHNTAFFERQEIFSTPI
                     LFEQKALTIKKFEVYFPDFMGSARQELAQYRQAIGQHDHPEQLEHLMYTILTHAENLS
                     TQLLENRPPIKVLIISNFDHAISLTFVDMLSYYCNNRFTFDIWDELKTSPEILNQTDY
                     DIIVSNFYISGITKKFICRNHLSIMNLVNHLNTLSNEIHLSNTL"
     gene            complement(36838..37566)
                     /gene="yaaA"
                     /locus_tag="VW854_000728"
     CDS             complement(36838..37566)
                     /gene="yaaA"
                     /locus_tag="VW854_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEV0262002.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQTVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
BASE COUNT        10575 a         8390 c         6894 g        11758 t
ORIGIN      
        1 agtaaccata tatacggcaa ggcgacgctg acgtggtttg aagagatttt cgaagagtag
       61 caaaatggaa aaaggagtga gtgaagcaca tcgcctcccc actccttttt ctgtttttag
      121 gctgtttttt caaccttcaa gatttttaca tcatagctac caacaggcgt ttcaattgtt
      181 gctgtgtcac ctgttttctt gccaatcaag gcctgcccaa ttgggctttc atttgaaacc
      241 ttacctgcaa aggcatccgc accagctgaa cctacgataa tataaacttc ttcttcgtcc
      301 tcgccaattt cttggatggt gactgtttta ccaatcgcta cttcgtcctg ggcaactgcg
      361 tcgctattga cgatttcagc atagcggatt tttgtttcta agctagagat ttgtccttcg
      421 acaaaggctt gttcatcctt agctgcttcg tactcactgt tttctgaaag atcaccgtat
      481 gaacgggcaa tcttaatgcg ttctaccact tctggtcgac gaaccaattt caattcttct
      541 aattcttttt caagtttctc cttttcctca agggtcatag gatatgtttt ttctgccatt
      601 tttctcaact ttcttctgat aatattttct aaagaaaatt atgtgaagta tcacataatt
      661 ttagtttgtt tagtttaatt tgctgttgac atgttcagcg acattgcggt cgtggtcttc
      721 ttgattgtta gcatagtaga ccttgccttc tgtgacatct gctacaaagt agaggttatc
      781 gctcttagtt tgattgatgc ttgactcaat cgcatccaga cttggactat cgactggacc
      841 aggcatgaga cctacatttt tataaacatt ataaggtgaa tcaatgttgg tatcaatcgc
      901 aacatcctca gctagactga tattttgccc cagttttcct tgggcataca agattgcaat
      961 attactttga agtggcatat cacgattcaa acgattgtag aatacacctg caatgagctt
     1021 acgatcttct gtcttggcac cttctttttc gaccaaggaa gcaatggtca acaactcatt
     1081 gacagtcaag tttttagatt tgatagtact atagtaagga gataggttct tatccatagc
     1141 agctaacatc tcatcaatca agctctcaat agttgtgctt tccttgatag agtatgtagc
     1201 tgggaaaagg tatccttcca aacgataacg cgcaccgctg tcttttacag gcaaactttc
     1261 cagtaaagta ggatatttcg ctactgcttg actgataaac gtctcatctt gaactttagc
     1321 caagaaagtc tccgctgtca aagactcttt gaagtcacct tgcaattgac ccacagcttg
     1381 agcaatctga tccaaggtat aaccttctgg aattgtcaaa gtcgcaagta caggttcttg
     1441 cggttcatct gttccacctt tttgcaactc tttgagtaag tcttctgtac tcatactctt
     1501 ttgcaaattg tagtaacctg ctttcaagtc ggtataattt ttatacttgg cataaaaact
     1561 aaaaatcaga ccatgcttta ccaaaccagc tttttcaagc gtcgtaccga tttcttgaac
     1621 gtttgaacct tctggaattc caaccgtcac atatttctta gaattagcat cgataggtaa
     1681 caaagaatct agcacgtact ggtaaccaaa gtaaccacct gctgagagca aggcaaggaa
     1741 tacaaggaat gaaatcacaa aagctttcac atgtgacttc ttctctttct taggcttaac
     1801 gggttttgct ccttctctac ggctacgacg tgatgtatcg ctgatgatat ccactgtttc
     1861 tgtagcaggc gtaggtgttg ctggacctgc ttgttctgct tttttctctg ccgtcgtata
     1921 ggaaacagct acccttgttg gggtttcatt gtattctctt tcaagtttct taggtctaac
     1981 aggacctgga cctggtcttg atccactttc ttccgctgga gaagaaggta catcttgact
     2041 tggatgactt ggaacaccag gagtttctct ttgaatctca tctgctggag aagctggtac
     2101 accttgactt gggtgagtag gcacggtagg agcttttctc ataatctcct ctaccgttga
     2161 caaggaatca gccatgagtt cttcagctga aggttcattt gcaggagtgc gaactactgc
     2221 ctcatcttct ttcagaactt catcatagcc ttttactttt tctaaatctc tcagaatctg
     2281 ctctttaaag cttaatttct cttcttctct tgacttttca ctcaaaagtt tttcctcctt
     2341 gttgataatc cataatatta tatcttaaaa gtccaaaaaa agcaatctat gatacttttc
     2401 ctaacttatt ttttcatttc ccagaccata tcataccatg tttcccctgc aaaggttgac
     2461 tgggactggc cttcgttgac aaatccatgt ttttcataat aggcgatgag atagtcatga
     2521 caggttagat taatgccatc tctctcatct tcaagagcaa cttctttcaa ggctgtcagc
     2581 aatttctgac ccagtccgag tccctgtgcc tccttggcaa tagacagaca ggtcacagag
     2641 atataaccac caggctcatg actatagtct tttatttctt ctgtaaaaga ctggtcttgc
     2701 agatggcggt gcaggtcaac tggtccttcg atataaccca tgattcttcc ttctttttca
     2761 gcaaccagaa aagaggtctg aatttctctc aaatgtgctt caaagacaga aggaggaatg
     2821 gcttcttcga ccgaaaaatt ttcaaattca agttcaacaa tccgatccaa atcttctaat
     2881 cttgcttgtc tgattttcat tgttcctcca gataaaaggg attaaaccaa atcatactat
     2941 agccctggct agttgcatag agcaaagttt cttcttcatc aacaaaaccg ttcattccaa
     3001 aataggaaag cagctcatca ggactctcca aacgaatcac tttgtaatcc agctcaactg
     3061 ccacctcttt caaggctgca agaagaagtg ttcccaggcc ctgtctctga tggtcagact
     3121 cgatgactaa agaatgtact tttagacatt gcggattgtc tgactgggga cttgataaaa
     3181 tatagcctaa aagttgattt tcatccctag ctagaagaaa ggtatccgca cacttacgga
     3241 tactttcttc taaaatatgg gaaagttgct gcttttcagc tggaaaagac gaggtctgaa
     3301 gtgcccctat ctcaggcaaa tcagacttgc ttgcctgaat gatcttaatt ggaatttcca
     3361 tgggaacatc ctattgaaca ttgcttgtca agttagacaa gagacgctca aatgagtatt
     3421 cataggtttg gatgtctcct gctcccataa agacgtaaac agcattgtca tggtctagga
     3481 gtggagaaac attttcaaca gtaatcactt ggtgtttttt gttgattttg ttggctaggt
     3541 cttctacctt aacgtcacca tgatctactt cacgagccga gccataaatt tgcgctagat
     3601 aaacagcatc tgcttggttt aaagcatggg caaagtcgtc caacaaggca atggttcttg
     3661 taaaggtatg cggttgaaag actgctacaa tttccttgct tgggtatttc tgacgagccg
     3721 catccaaggt cgcaataatt tctgttggat gatgggcaaa gtcatcaata atcactgtat
     3781 cattgacaat tttctcagtg aaacgacgtt taacaccggc aaatgttttc aagtgctcac
     3841 gcaccaagtt caaatcaaat cctgctgtgt aaagaagacc aataacggct gtcgcattca
     3901 tgatattgtg acgaccaaag gttggaatgt ggaattgccc caagttttgt ccacggaaat
     3961 gaacggtgaa ggttgaacca gttgttgaac gaagaagatc actagctaca aagtcactgc
     4021 cttcagcttc aaaaccataa taataaattg gtgcatcaga cgtaatctta cgcaattcag
     4081 catcttcacc atagacaaaa agacccttgg taatttgttt ggcatagtcg ttaaaggcat
     4141 tgaaaacatc ctcgagactt gtgaaataat ctggatggtc aaagtcaatg ttggtgataa
     4201 tagagtattc tgggtggtaa ggcatgaagt gacgctcata ttcgtcagat tcaaagacaa
     4261 aatatttggc attagccgaa ccacgacctg tcccatctcc aatcaagaag ctggtatctg
     4321 taatgtgaga caagacatga gacaacatac ctgtcgttga agtttttcca tgtgctcctg
     4381 ctactcccat gctaacaaag tcacgcataa agctacctag aaactcatgg taacgtttgt
     4441 agctgatacc attttggtcc gcataggcaa tttcgacgtt gttatctgga cgaaaggcat
     4501 ttccagcgat aatttccata tcaccgtcta gattcttttc atcaaaagga agaatggtaa
     4561 ttcctgcctg ctcaagaccg cgttgggtaa agtagtactt ttcaacatct gatccctgaa
     4621 ccttgtgccc catctggtgc aacatcaagg ccaaggcact catccctgat cccttaattc
     4681 cgataaaatg atatgtcttt gacatgtttt ctcccctatt ctgtcattct ggtcagattc
     4741 aactcttggg caacccgacg ttcttgttct gtttgtttac tttttttatt gtagatttgg
     4801 ctcttcttta gaaaatcata attgtttttc tttggagcag gtgttgacac ttcttcattc
     4861 ttggtaggga tagaatgaac ttcttccgcc aagatataat gagactgggt caatttttgg
     4921 ctatatttga caaattcacc aggattttcc ttttggaaag gagttgtcgg ttgattgccc
     4981 tgtctaacta gactgggctg agaatgacgt ctcgcaaggc tgaaatcctg agttaggtag
     5041 ttagcagagc gtttcttttt caagtccgca cgcgcttctt cacgcgccac ctccgcatag
     5101 ctctttcctt cttttttaac ccctaaagga gcctttttag gtttttcgac ttgcttttca
     5161 atcggtttta ctggtttttc ttcagtaata ggagcccatt ctaaataatt tttatctcga
     5221 tactcaccct tgatattact gatcagatca gactcatcat agagattcat gactggcatt
     5281 tcagtcaaca tgacctcgtc atctgacacc aatggaaatc gttcttgttt cattttctat
     5341 ttcctttcaa cacttcatta tagcgtattg tcttgatttt tcaagtgctg gcttcagaaa
     5401 ttcccaaaat ttctctaatt tctgctaggg tcagactacc acgtgactct gtgccatcca
     5461 atacttgtga caccagatgt ttcttttgtt cttggagttc ctgaattttt tcttcaatgg
     5521 ttcccttggt caccaagcga tagacctcaa ccgtttcttc ctgacccatc cgatgggcac
     5581 ggccaatggc ttgcgcttcc accgcaggat tccaccaaag gtcaaccaag atcactgtat
     5641 ctgcacctgt caggttcaga ccgaccccac cagccttgag ggaaatcaga aaggcatctc
     5701 tttctccttg gttaaaggcc ttggtcatgt cttgtctttc cttggctggg gttgaacccg
     5761 taattttaaa ggatgtcagg cccaagtctg gcagttcttg ttcaattttt tccaacattc
     5821 ccttgaactg agagaaaatc aagacacggt gtccgccgtc tgccacctgt accagtaggt
     5881 ctcggagact atctagtttg ccgctggctc cctgataatc ttccataaac agggcaggag
     5941 tgtcacatat ttgacgcaag cgcatcaaac cagataaaat ttccacacga cttcgctgaa
     6001 attcctgttc tgacacttga gccagatggt ctcgcatctg ttgtaactgg gcaaggtaaa
     6061 tagccttttg ctggtcttcc agttcatttt tataaaccac ctcaatcaag tctggcaatt
     6121 cagtcagaac ttcttctttc ttgcgtcgca tcacgaaagg cttgataaac tgagccactc
     6181 gctctgctgg caatttcata aattctttct tgcttggcaa aagtccgggc atgacgattt
     6241 ggaaaataga ccacaactca cccagatggt tttcaatcgg agttcctgac aaggcaaaga
     6301 ccgacggcac cacaaattgt ctcaaggtct gggcaatctt ggtctgggca tttttcatga
     6361 cctgagcctc atctaagaaa aggaagtcaa aggccatccc ctgataaaac tcactgtcct
     6421 gacggaaggt ggcatagcta gtcacataga tttgatggct ctcggcaaga atctcctcac
     6481 gacttgcttt caaaccatga acaacagcca catccaactg tggggcaaat ttctgaaact
     6541 catctgccca gttgtaaatc aaacctgacg gagcgagaat caaaacccga ctttcttttg
     6601 tcacttgact agtcaaaaaa gcaatggtct gaagggtttt cccaagtccc atatcatcag
     6661 ccaaaatccc accaaaacca taatgatgga gcatctgcaa ccagccaatt cccttttcct
     6721 gataatctcg caagtcagcc tgaacctgag ttgcctgtcg aggaaagtcc tctggatgtg
     6781 tcaaatcctg ggccagattc tggaattctt gtgaaaaaga aacacggtct cgcccttcaa
     6841 agagatgagc taaactgtag gccaaggatt tccgagcctg caaggtccca tcttttaatt
     6901 caaattgccc cagttcctgt agattttggc gaattttctt ggtttcttca tcgaaaaagt
     6961 aaacttgatt agacgaatca atataaaaat cctgattggc aaccaaggcc tgcatggctt
     7021 ggtcgatttc ctcctgggca atatcttgaa aatcaaactg gatttccaag agacctccct
     7081 tggaggcaat ctgcacctga ggactcgcta ggctataaag ctcttctagt ttatctgata
     7141 ggtcaacatg cccgagtttt tcaaagactg gaatgatatc atgaaaaaaa tgatagacag
     7201 actccgcttt taaggcctga cgccaagatt gaaaatcggc ttcaaagccc gcagccaaac
     7261 agacttggaa aattctttct tctaagtccg cgtcacttga aaatggtaat tcttctagct
     7321 cttgtcggct agatacctgt ctatttccat aatcaaactg aatttctaaa cgaatccgat
     7381 tatcttcttc cctgtcaaag taaaaagagg gcgcaaaagt tttgatttgt agacgttctg
     7441 gagctgaaac ggtgcccatc tggataaaaa gagtcagaca ggaggccaat ttgtctcgat
     7501 cactgctatc aaattgcagg tatttctttc cttgttgacc cacaggtaac gctttaattt
     7561 ccttgagaag acgcatctgc tggtctgtta aaaaataaac ctgaccttta tggaaaagta
     7621 ctgctccctg ataaaagaca ttgaccctag gactctcact gatttccatt tcaaaatagt
     7681 ccgagtattc tgttactgta aaggcaaata gattggcatc agcatgcata tcctgaaaaa
     7741 gcagggtttg gtagctatcc acttgatggt caaattgaaa atggggcaag gccatcagta
     7801 aattcacacc ctgctcaaaa aaggtcagag ggaaaaagag gtgccgacct tggttttgga
     7861 aaaagaggtc tgaaaccagc ccttcctccg ttagtccgtg caagaaagtc aaaagttctt
     7921 ggctggcatc atcaaaggct tcccaagaaa gagactcctc ataaatcttg ccaatcatat
     7981 acgactttct ctgctcgaca atccttaaaa aaagtggaat atcccgaatg acatagtatt
     8041 tttggctatt gatttggccg attctcagag tccacaagat atgattggtt cctgcttcca
     8101 cctgacccac agctgataac tcataggcgc attctgattt tggagataaa attcgatcca
     8161 aaaacttgcc acccaaggtc accttggttt caacagcctc tttttcttca tgaccttctt
     8221 ccagactcca caagatttcc tgaccacgct catcattttt cagaaaatgc tctagcgctg
     8281 ccaaatgcac acagtaaccc ctcttttgaa aaaaatcaca ggcacaaaaa accaaatcat
     8341 cctctaaact atagcgcagt tcttcttctg caacgcgagc gtagagccga ttgttctttt
     8401 ccttgatgat atcaacctta ccagtttcat aaagggcaac accttcgata cgaattttcc
     8461 ccggaatcaa tttagccata ttttcacctt taccttatct ttttattata ccatattttc
     8521 gcctatgaaa atagccttct aggaagactt ttctcctaga aggctggatt tttaacgttt
     8581 ggcaaaagta gccacaatcc gctgacagac ttcttgcaac agagatttgg gcatagctat
     8641 attgatgcgg gcatggagac ttccttcctc tccaaaatcc aaaccacggt tgaggataac
     8701 cttggcttca tttctcaaca actcttgcaa tgtttcatca gtcaggtcat aagctgaaaa
     8761 gtcaagccaa atcaagtagg taccttgcgg tttcatgacc ttgattttag tctcttttcc
     8821 aaatagatcc accacataat tgatgtggtc ttcaaagact tgcttgagtt cctctagcca
     8881 atctttaccg tatcgatagg cagcttctgt cgccaaataa cccaagcctg aaatttcatg
     8941 ctgattattg gccaacaggc gtttctggaa agccagtctc aacttaggat tttcaatgac
     9001 tgcataggaa ttttttgttc cagcaatatt aaatgtttta gtggcactgc tcaagacgat
     9061 agcaaaattt ttgaaggcag gattgatggt attgaaagac tggtgtttgt gaccaaagag
     9121 ggtcaaatct tggtgaatct catccgaaac taacaaaaca ccgtgttttt ggcagagttg
     9181 gccaatcttc tccaacactt ctttttccca aacacgtcca ccaggattgt gagggttgca
     9241 aagaacatag agtttaacct cctcttccac caaatccttt tcaagttggt caaagtcaat
     9301 ctcaaacaga ctatcctttt ccactaagga attagtaatc aatctacgat tattcaactt
     9361 gacactgcga gcaaagggtg ggtagacagg cgtgttaatt aaaaccgcct cgccttcttt
     9421 tgtaaaggtt tgaatagctg ttgagatggc tggtaccaca ccctcgataa agacaagagc
     9481 ctctttgtca aagttgtaac cgtattgtgt agcttcccac ttttgaactt ccttaattaa
     9541 gtcttcactg gcataggtat aaccataaac cagttggtct gcgtaagttt gcacggcttg
     9601 gcggatttca ggcaagagca caaagtccat atccgctatc caagctggta gaacttcact
     9661 atccgtttct gtttctttcc atttataggt atggtgccct aaacggttgg gcaggcttgt
     9721 aaaatcatat tttcccatct ttgtcttatc cttctaaggt ttggcgcaaa tctgcaatca
     9781 aatctctagc atcctcaatc ccaatagaca aacgcaagag gtcatctgtc aaaccataag
     9841 aatggcgtac ttctgctgga atatcagcat gagtttgagt cgttggataa gtaataagac
     9901 tttccactcc gcccaaactt tccgcaaaag agaagacctt gagactgttc aaaatatgag
     9961 gaatgcgtgt ttcatcggct actttaaagg aaatcatgcc tccacgacca gtgtagagaa
    10021 cttccttaac tgctggagaa tccttcaaaa aggcaaccac ttcttgggcg ttagctgttg
    10081 agcgctccat acgaagagac aaggtcttga gaccacgaag caactggtag ctgtcaaatg
    10141 gagacaagac tgcccctgtt gtattgagat tgtaaaaaag cttctcgtat agttctaaac
    10201 tattggtcac aaccactcca gccaagacat cattgtggcc tgctagatac ttggttgctg
    10261 aatggagaac gatatctgct ccatcttcaa tcggacgttg gtagataggg ctatagaagg
    10321 tattgtccac caccactttg gcacccttag catgagccaa ttttgctagt ttttcgatat
    10381 caaattccaa catcaaggga ttggttgggg tttcgatata gagaacatcc acatcctttt
    10441 ctaactcggc aatcaactct tcttctgtat tggcataggt aaaatggaaa cgaccttcct
    10501 gctccacttg gttaaaccag cgaaaagaac caccgtaaag atcacgcact gccaagacct
    10561 tacttcctac tggaaagacg ctaaaggcca gtacaatagc tgacatccct gagctagtcg
    10621 ctagggcata gtctgctgac tcaatagccg ccaagacttc ctcagcctta ctacgagttg
    10681 gatttttagt gcgcgtatag tcaaacccag tagatcgacc aaactctgga tgctgatagg
    10741 tcgttgaaaa atgaagcggt gtcaccaatg cacctgtcgc ttcatctgac ttaatacccg
    10801 cctgggccaa aattgtgtta atgtgtaatt ccttgctcat acaatacctc caaatctata
    10861 gtaactattg taccacttat tttcatcaac tcattttctt cttttcaaga gctagttata
    10921 gtttcaaact atataaaaag ggagtttttc ctgctccctt taatagacta taaaatggtg
    10981 aatctcaaaa gacacttcac tctatcattt gctcctgcac aaaacgagca taacgctcat
    11041 gattttccag tagttcctta tgagttcctg agccagtgat tttcccctcc tctaagaaga
    11101 aaatacaatc cacatctttt accgttgaca aacgatgcgc tataatcaca accgtcttct
    11161 cctttaatac agaatagagg ctactgataa tcgcatactc agaatccgca tcaagattag
    11221 cagtggcttc atcaaatata agaatttcag catcttttaa gtaggctcta gctatttgaa
    11281 gtctttggct ttgccccccc tgacaagagt cgtccgcgtt caccaacttc agtatctagt
    11341 ccctctttca tggagcgaat ctcatcacct agtgatacta agtctagcac tttcatcaat
    11401 tcatcatcag ttactaagcg attcaaaccg agacaaagat tgtcacgaat actgccagat
    11461 aagactgcat tattttgtga aacccaagcg attttacttc tccattcttt taagttaaaa
    11521 tcatatatac ttgattgctc cattagaata tctcctgaaa gcggtttata aaactgctct
    11581 aacaaacgca caatcgttga ttttcctgat ccagatggtc caacaaaagc aattttttgc
    11641 cccttgaaaa ttgaacaagt aatatccttt aagacaggtc gattttcatc ataaccaaaa
    11701 tagacatggt taaaattcaa cccttgtcct gataccgatt ttcctcactc aaatttttct
    11761 ttaggaactg caagcaagtt ctccagtgca actgaagatc ccttgctcct agaataaaca
    11821 gttacaaaat tagctatatt actaatagga ttaagtaatt gaaagaggta aatcaaaaac
    11881 gaaaccaagg ttcccacaga tatatatcct gcgctgaccc gataaccccc ataggttagc
    11941 atcacagcta tagccgcaaa gataaataag agagcaaacg aggtctcaaa agaagtaacc
    12001 ctatctgatt tcagtgaatt gttttgtacc ctttcaatac aattatccaa aacatcctgt
    12061 acacttttct ctgcttggtt agtcttaatt aattcatgtt cttgaatctt ttcagtcaat
    12121 tgccctgtta aaaatccgat ttcctcctgt aaacgacgac tatacttttc actgatattg
    12181 gaaaggggca agataataaa catcatacaa ggaagagtga tgaataaaag tagagaaaga
    12241 ttccaatcaa gactaaataa gactacaatg gaaccaagta ccataactaa actcagaata
    12301 atatttggga aagtcgtaat taaaaactca cgaatgacac tcgtgtcatt gacaatggca
    12361 gaagtcaact ccccacttta gctcttatca aagaaggatt tctctgcata aatcaacccc
    12421 tctatcactt ttttcctgat ttttgctatc tttttttcac ccgattgact aaacagatag
    12481 taaccaatag aagaaaacaa ggcttgacca ataaaaatca aaaacgattg aaatactttg
    12541 gaacctatat tttcaataga actcccatct attaaacctt ttaagataag gggaagcaac
    12601 aaagcaagta gactagacag aacaaggaag aaactcccca taatcacctt agtatctact
    12661 cttaataatt ttaatttcat aaatactcct tataatattt caacggataa agtcgggaat
    12721 aactcaattt gaggataaaa tctaataaat cttcctataa caaaacgcat aacatctagg
    12781 attttatata cctgatatta tgcgttttta agcacaaaga cttcttacac aaacttatct
    12841 acaattagat tttatttgac atgttttgcc aattcttctt gggctttttt attggattct
    12901 tctttttctt tcaaccattt ttctctagct tttgcatatt cgtctgttgt gacaatctta
    12961 tcttgtactt tgaggtattt atatgattca atcccttttg taccggttaa accataggca
    13021 gcagcaaatg gtacggttct tctcaatgat ggtgttcccc cacgcgaaac acttggaaga
    13081 actaaagaac tatcaatcaa ccaagcttga atatcagcat atttctcata acgtttggct
    13141 ggatcttgct ctttattagc ttcttccaac atttgagtat agacatccag tccaacttcc
    13201 ttagccttgt cattggcttc tccaggctct agtccaagat tttgtagaac tcctccacta
    13261 ttagtattaa aaatatcgag ataggttgac gggtcttgat aatcaggtcc ccaaccgcca
    13321 tgatataaat catagtcttt ctgagcagct gtttgagcaa agtagcctga actgtcaaac
    13381 tcatctgatg ttaattgctg aatgtcaatc actacattat cagaacctaa aacagattca
    13441 attgattgtt tgatagaact aactccttgt atgcctactt tatctgttac ttccacagtc
    13501 ttatccaagt ggattgggaa ttgaacaccc tttgcttcga gttctttctt agcttccgca
    13561 aacttagcct tggctttctc aggattgtag taagggtctt gaccatccgc aaagttgata
    13621 ccttgccatt ccttaccata gttgaccatc ttagaggcta caacttcacc aaagtctttt
    13681 cccttgatac tgacaaagtt tggaggaacc actaggttac gcaaaatctt tgttgcacct
    13741 tctttccctt cagactgagc cccataagat gttctgtcaa aagcaaaatt gatagcctga
    13801 cggaagtttt tattgagaac tgcttcctga gtcgatttct tttcaatgtc acttgtttta
    13861 gaagtataat tgtaagactt cctatctagg ttaaaattaa agaaatatga agttgaattt
    13921 tgcatactat atatgatatt gtttttgtat ttttctttaa tcccttcata gctggagctg
    13981 ttaggaaaaa gacgagccgt agtataagca ccagctgtaa aattacgttc cagtgattct
    14041 tggtcgctac catcatagta ggtcaatttc acatcgtcta caaagacatt cttagcatcc
    14101 cagtaattag ggtttttctt atattcaata gcagattttg agacaagtgc tttcatcaag
    14161 aaaggtccat tgtacaaaat actagatgga tccgccttcc caaaatcatc cccttttgat
    14221 ttcaggaaat ctgcattaac aggaaaaagt atcgttgcaa gtgtttttga attccagtaa
    14281 agttctggtt taaccaaagt atattgaacc gtttggtcat caagtgcctt gacaccgaca
    14341 gttgaaaagt cgcttgtttt accagtgata tagtcatcca aaccagcaac agagtcctgc
    14401 actagataca aagcttctga ttttttatca gctgcatatt gcaaacctgt cacaaaatcc
    14461 tgggcagtta caggcgcata ttcttctccc tcagaagtaa accacttggc atccttacga
    14521 agtttgtagg tataggtcaa accgtcctga gaaacagtcc aatcctctgc taatgatgga
    14581 ataatattcc catattggtc attttctaat aacccgtcta ccaaatttgc aacaatatcg
    14641 gatgttgctg cgcggttttc tgctagatag ttcaaactag atggatcact tgaataaaca
    14701 tagttatagg tttttgatgc agtgctagaa tttccacacg cgctcaataa aactcctgta
    14761 cccaggacaa gacctgccaa ggttagatat ttgctcttag actttttcat ttccagaacc
    14821 tcctgcttta aatatttgtt ttattataca actctgggtt tgtttagtca attgtttttt
    14881 ccaaaaaatc aaaaaaacct cattttctat ttttgaaaga taattttagc tattttgacg
    14941 ctaaaaaagg aataagtttc aagttctcct aaaagattgt tcaacttatt ccttttattt
    15001 ataaatgcta ccttacgaaa gcttaaattt tgctcgcagg cgatctgctt gggcttttcc
    15061 tattacctta tctaataaat aggtacgcag actcataacc atataaagtc caccccccat
    15121 ggcaccgaca agagctacat aaaagaagct ccacaaacgt ccacttggtt ggaagaaaaa
    15181 tcctaacagc cactggatgg ttcctattaa cagaaacatg actagggtca gcaaactgat
    15241 taaaatggtt cgcttcaaaa tcaccttgcg cttgacacca gttactttac aaatatcccg
    15301 atacatcaag acgttaggaa tgatgagagc aatggttgtt gaaatcaaag gaccataact
    15361 gtggaagagg gcgatggtag gtagttgcaa gactagcttg gcaatagaac catagataaa
    15421 atagagaacg gccttgcggt tgcggaacat ggcctgaagc attggagaca agaccatgta
    15481 caagcctaaa ataatagact gcaaaactgc aaagacaaat aagcccagag ccaaactatc
    15541 tggcttacca tagaagaccg tataaagagg ttctcctacc ataaccactc caaccgttgc
    15601 tggtagcaag aacataaaga gtagggtgag actgtcctga acgagacgag aagctgcttt
    15661 caagtccccc ttgacatagt tttccgtcaa aagtggcaaa ccaacactcc caatcgaaac
    15721 ccctacagaa atcaaaatca tcgtgatttt attaggattg gctgagaaat aagaaaacat
    15781 gacaaccaag tcctcattgc tgtagttggt aaaccagctc atactattga taaaggtcag
    15841 ctgatccaaa atctggaaga gctggatggc agaccctgtc aggataaaag gaatggcttc
    15901 cttaatggta tcaaccaaga gacgcttact gttaatctta tctcctgttt caaagactct
    15961 tttgagtgaa ccttcttggg caaggaaata aatcaagact gcaaaactgg ctaccatacc
    16021 gacaaaggca gcaaaggttg attgggtaac ggctgctaga taatctcctg aaccgagctt
    16081 cataatgata aaggttgcta ggagcatcca gataacacga atgacctgct cagcaatttg
    16141 gctcatagca tagggtttga ggttattcat cccttggaaa aatcctcgga taacactcat
    16201 agacgggaaa atcaagactc cccaagccaa gctttgcatg attgggatca agtctttgcc
    16261 cacgccagac aagtctgcta gccaaggagc aaagacatac aagactaaag caaaaaccag
    16321 gcctagtcct gtcataaagc ctaagaagct ccgaatcagg gcaaagctat gctcttcttc
    16381 tcgcatggta ttatacttgg caacttgctt ggccaccgca actggaatcc ccgctgttga
    16441 aaccaacaag aaccaagcat agatattgta acccatggta aagagaccat ttgccttagc
    16501 tgcataagcc cccatccaga tgtaccaagg gataatgtaa acagccccga gtaggcgact
    16561 gataaagtta ctagccgtta gccaagcagt cccccgtaac atctgggcct gctggtgatt
    16621 gttttcgtgc gacatagatt cctcattcaa ttttgataac taggaaaaac tccttatctt
    16681 ccattataaa cttttccttg ttacttgtaa atttacgact ttcggtttac aatagaaagt
    16741 atgattaaga ttgaaaccgt attagatatt ttaaagaaag atggcctttt tcgcgaaatt
    16801 attgaccaag gtcattacca ctacaactac agcaaagtta tttttgatag catcagctac
    16861 gacagccgaa aagtaacaga agacactctt ttttttgcaa aaggcgctgc ctttaaaaaa
    16921 gaataccttc tttctgctat aacacaaggt ttagcttggt atgtagctga aaaggactac
    16981 gaagtcgata tccctgtcat cattgtgaac gatataaaga aagccatgag tttgattgcc
    17041 atggagttct atggtaatcc acaagagaaa ctcaaactcc ttgcctttac tggtactaag
    17101 ggtaagacaa cagcaaccta tttcgcctat aacatcttat ctcaagggca tagacctgct
    17161 atgttgtcga ccatgaacac aactcttgat ggcgagactt tctttaagtc agcgttgaca
    17221 acccctgaga gtattgacct ctttgacatg atgaatcagg ctgtgcaaaa tgaccgtacc
    17281 cacctcatca tggaagtctc cagtcaagcc tatctagtcc atcgagtcta tggactgacc
    17341 tttgatgtag gagtctttct taacatcact cctgaccata tcggcccgat tgaacaccct
    17401 agctttgaag actatttcta ccacaagcgt ctcttgatgg aaaatagccg agcagtcatc
    17461 attaacagtg acatggacca cttctcagtc ttgaaagaac aggttgaaga tcaagaccat
    17521 gatttctatg gtagccaatt tgataaccaa atcgagaatt ccaaagcctt tagcttttca
    17581 gctacgggta aactcgctgg agattatgat atccaactca ttggcaactt caaccaagaa
    17641 aatgcagttg ctgctggact tgcttgtctc cgtctcggag caagtcttga ggacatcaaa
    17701 aaaggcatcg ctgcaacccg cgttcctggt cgtatggaag tcctcactca gaaaaatgga
    17761 gccaaggtct tcatcgacta tgcccacaat ggggatagtc tgaaaaaact catcaatgtg
    17821 gttgaaactc atcaaaccgg aaagattgct ctggttctgg gatcaacagg aaacaaggga
    17881 gaaagtcgtc gtaaggactt tggcctcctc ctcaatcaac accctgagat tcaagtcttt
    17941 ctgactgctg atgaccctaa ctatgaagac ccaatggcca ttgcagatga aattagtagc
    18001 tacatcaatc atcctgttga aaagattgcg gatcgccaag aagccatcaa ggcggcaatg
    18061 gctatcacaa atcacgaatt agatgcagtt attattgcgg gtaagggagc cgattgttac
    18121 caaatcatcc agggcaagaa agaatcctac ccaggagata cagccgtcgc agaaaattat
    18181 ttataagaat agaaaaaatc aagggaaaat gaaacccttg attttttcct attatttagt
    18241 aaaaacgttt ttcaaacctt caacggcacc ttctacagca ccttttgcgt cttctacaac
    18301 gtcttttgcc ttagcaactg ttttttcaac agctccttca agttctgtct tgctgtctcc
    18361 agtaacttta ccaaaacctt ctttgacagc gcctgttgct tgttccaatt tgttttcaag
    18421 tgacatatga ttgtctcctt tagtttattc cgatatgtgt taccggttac atatagttta
    18481 taccgaatac taaggaaagt caaacaatgt gctcagaaac aagaaatcac gctaagtaga
    18541 aagttaatct ctccttatca aaatcgatag ccaactcata ctttctatct tcattttgca
    18601 caatatagcc taagacaacc aaactgtcca caaagatatc acggcatttc tgcataagct
    18661 ggtctttttt gagaaatttc aacaaaaaag ccgtcatata cttgagtgca tactcaggat
    18721 taacatctcc taaaatgtca tagagctcct gctgtttttc tgtcaaagga tactgctgtt
    18781 tgaccttgta aaaataattg gacagggtca ttttaatgcg cgcaaagtcc gtctttcaac
    18841 taaaatagct gcattgattt gattgcgcaa ttccgtctca aaactctgct ccaacaaggc
    18901 ttgatagacc ggactagctt ctctgacaaa aatctcttga tccagttcaa gactatcaag
    18961 tgattcaagc gtaggaaaat tgaggtaata gcgcttattt tcacgtagaa tcaagcttgc
    19021 ctttatatac tcttccatga gtttatcaac tgcaacatct ggaaattgag ccttaatttc
    19081 ccgtagaatc acatcctcat actggtccag atagcggatc aattctccaa aaatggctgt
    19141 ctcgtcaaac gagatggatt aaaaatctga atcatgaaga ttcctttctt tacattgtaa
    19201 cagaggcgat gctgccaact gactaggatt ggtcccagct tggttcagag gaacaggcag
    19261 acctagttct atgtaatact ccctccaaaa atcactaatc tcctgttccc catctccaaa
    19321 tttcatagga atagtttcaa aaataggtag gatttctgct atatactggg agcagtagaa
    19381 accagctgca tctggataga aagaagcatt gtagggtgct ccaagatgtc tgcaagttct
    19441 ttccttcacc gactggatat ccatttctgg gtaaacatag aggtcgtaca aatgattgga
    19501 ctcaaagaag tctgccggtt cttgacagac aacaccagcc tgtccactag catgataaat
    19561 catcccatcc aaataaatgg caacatggct atagttacct gtggaagtct ggatggcctg
    19621 tcccatgtct gacccatctc tcacaaaaat caaatcgcca ttttctaaca tacttcactc
    19681 ctagaaaaaa aggagccgaa actcctttcg atataaggca aactagactt tccgattttg
    19741 agctgacacc caaaatctta cgcattaaag ctttcagtta attgaggtac cacttgtttc
    19801 ttacgtgaaa cggcaccagc aaggaaggca tgattgtttt caagtttgaa attgaaagcc
    19861 gcttcgacct tgtccatatt ggcaccaaga gccaatattt ctgagtttga gttgacgata
    19921 tctgtaatca tcaagacaaa gtcagagtag ccgtttgatt cgttggcagc ttgcattgca
    19981 gcttcaattt ctgcttggcg ttccaaaact tcagcgatgt caactgtgtt cacttgggca
    20041 acacggacat tatttccgtt gagttcaaaa gtcttagcat cgatgtcaat caattcttca
    20101 gcagatttgc tagccaagtt ggtaccagct ttcaacattg ccaaaccata ttcttccaag
    20161 tttacaccag ccaattcagc caattcagga gcaatgattt tatctgttgg gtgtgttgtt
    20221 ggtgatttca aaagaagggt atctgaaatc aaacctgaaa gcatcaaacc tgcaatctct
    20281 ttaggcacag ctacaccatg ttctttgaac atacggtaaa cgattgaaga cgctgatcca
    20341 actggctcca aacgcatgta aagtgggctt gcagtttcaa agttagccac acggtggtgg
    20401 tctacaacac cgtaaacttc tacttcagcg atatctgata cagattgttg gaattcattg
    20461 tggtcagtca agataacttg ctctgccccc tctgctttgg cagaagtgat aacacgtggt
    20521 gcttccacac caaaatagtt caagacaaag gctgtttctt catttggagt tccaagggca
    20581 acagcttccg tatccaaacc gtaagcttct tttgcaaggt aggcaaaagc tacagatgac
    20641 ccgatggcat ctgagtctgg attttggtga ccaaatacta gaatcttgga catgataata
    20701 cctcattttg tttattttct ttattgtagc atttttttcg ctttttgaca aaggtaagag
    20761 gagtcaaagg actccccacg attcttcaaa ataactgagt aaatttctat cttgattttc
    20821 agataaaaat tcttccttct gtggcctctt cttacgcttg aaaagagctt cagccgacat
    20881 ggcttcttcc ttactggcaa aaccttgagc atagataagt ttgactggca agcgtgctcg
    20941 tgtatatttg gctcccttcc cactattgtg gatagcgagg cgtcttctca tatcagtcgt
    21001 atagcctata tagtaggatc catcacgaca ctccagaacg tacatataag ccttatgatc
    21061 cataataaat ctcttcgatt tcgggcgtat aagagccatc atcattgtgg acaatcaaag
    21121 gaggtaagac cttaaagcca cttgttgagc catccttgat cgcctcaatc aaaagcatat
    21181 tggcttcctt ttctcttttt ggataaacaa actgcaggcg cttaggggct agattatgtc
    21241 gttttaacgt atccaaaata tccagaagtc gatcaggacg atgaaccatg gccaaacgcc
    21301 cattagactt gagaatactc tgggcactat gacagatttc ttccaaatta gtcgtgattt
    21361 cgtgtcgagc caagagataa tgttcactct cgttcagatt agaataagga tccaccttga
    21421 aatagggtgg attacacaaa atcatatcca ccttactccc ctgaatgtga gcaggcatat
    21481 ttttcaaatc atcgcagatg acctgcattt gctcctctaa tccattcaaa cggacagagc
    21541 gttcagccat atccgccaaa cgctcctgaa tctcaacaga caatatctgt gcttgagtac
    21601 gagtgctagc aaaaagcccc actgctccat tcccagcaca gaaatccaca atcaacccct
    21661 tcttaggaaa acgtggaaat cgtgataaga gaacactatc caccgaatag ctaaaaacct
    21721 ctctattttg aatgattttg atatctgtcg aaaagagctg gttaatgcgc tctcctgatt
    21781 ttaataattg ttcttcttcc atggtcctat tatagcaaat ttatattaac attacaaaaa
    21841 atataaaact ctaaactact tcttcttttt taaatggtgc agggcttctc cagtccagat
    21901 tggtagcatt cgtcgaaagg gagcaaaacc gtagttaaag cggtcgcttg aaaagcgtct
    21961 ccgtctagga aactggtact tttcttcctc caaagtgcgg atagaaagac tggctttccc
    22021 cgtaaattca tctaaatcca ctacctgaac ttgaacctct tcatcgactt tcaaggtttc
    22081 atgaatattt tcaataaatc ctgtccgaat ttctgaaatg tgaatcagcc ccgtatcacc
    22141 cgtctctaac tcaacaaagg caccgtaggg ctgaatccct gtaatacgcc cctttagctt
    22201 atcaccgatt ttcatcttag tcctcgattt caatagtttc aattacaaca tcttcaactg
    22261 gcttgtccat agctcctgtc tcaacagcag caatgacatc caagacagcg taagatgctt
    22321 catcagctaa ctgaccaaaa accgtgtgac ggcggtctag gtgaggtgtc ccaccttgat
    22381 tggcatagat ttctgcaatc ggttctggcc aaccaccacg agtaatttct ttcttagaat
    22441 aaggtaggtg ttggttttgc acgataaaga actggctgcc gttggtattt ggaccagcat
    22501 ttgccatgga aagagcacca cggatattgt aaagctcttc tgagaattca tcctcaaaag
    22561 attcgccgta gattgactcg ccacccatac cagttccagt tgggtctcca ccttggatca
    22621 taaagtcctt gataatacgg tggaaaatga caccatcata gtagccatct tttgaaagag
    22681 atacaaagtt agccactgtt ttaggagcat gttcagggaa aagcttgata cgtaagtctc
    22741 cgtgattggt cttaatagtc gcaagaggac cttctactgt ttcaatgtct acttgtggaa
    22801 aatgcaattc tttttctacc ataccaaata cttctaaggc atcaaaaatg ccatcttctt
    22861 ctaatgtttt tgtaatataa tctgcttttt ctttgatttt atcatgagaa attcccatgg
    22921 caacgctgat tccagcataa tcaaagagtt ccaagtcatt gagaccatct ccaaaaacca
    22981 tgaccttctc tggtttcaag ccaaggtgtt ccacaacctt ttccaccccc gtcgctttgg
    23041 agcctgaaat cggcacaata tcagacgaat gttgatgcca acgaaccatg cgaagtttgt
    23101 ctgagagact gtcaggcaag tgcaagtcat ctcccttatc ttcaaaagtc cacatctgat
    23161 agatatcttc tttttcatgg aaatcgggat ctacatctaa gtcgggataa attggattga
    23221 tagcttcact catcatatcg gtgcgagtcg acaacttggc atcatgactc ccaaccaagc
    23281 catactcaat tccttcttgc ttaacccaag agatatactc ctcaacatat gacttttcaa
    23341 tctgatgctg ataaatgacc tgaccttttt tatcttcgat ataagcccca ttcaaagtta
    23401 caaaaaagtc aggtttgaga tcacgaatct ctggaacaac accaaaaatg ccacgtccag
    23461 aggcgattcc tgttaaaatt cctttttcac gcaactgttt aaaaacagtg ggaattgtag
    23521 ttggaataaa ccctgtcttt gaattccgca atgtatcatc aatatcaaaa aagacaatct
    23581 tgatcttctt tgccttgtat cttaatttcg cgtccatctc actacctctt tcaatctaac
    23641 tctttccatt atatcataaa gtaggcaaat cccctatttt caaaaagttt atcattttta
    23701 ttttaatttc ttggatgaga aaaaagacat atttatgaaa aagctccatc gtgcttttaa
    23761 tgtgttctct tgttttcaaa ctcgtaaaaa gggagccact gagcctgact cgctctctca
    23821 tttcaaagct tgtgaaaaaa gacccgttgg ggtcttaatt cgctttcttg ttttcaagct
    23881 catgaaaaag agacccaacc gggtcttttc tttaatcttc gtttacgaaa ggcatcaaag
    23941 ccattacgcg agcgcgtttg atagctgttg ttactttacg ttggttttta gctgaagttc
    24001 ctgttacacg acgaggaagg attttcccac gttctgaaac gaaacggcta agaagctcag
    24061 tatctttgta atcaacatat tcaattttgt ttgctgcgat gtaatcaact tttttacggc
    24121 gtttgaatcc gccacgacgt tgttgagcca tgttttttct cctttataag tttagttgtc
    24181 catcagaatg gtaaatcatc atctgaaata tccaatgggt ttgttgctcc aaatggattt
    24241 tcattacgtg aaaagtctgg tactgaattt gtaggtgctg aatagtttgc agttggtgca
    24301 gagtaagctc cacctgtgtg accctcacgc acactacggc tttccaacat ttggaaattc
    24361 tcagccacga cctctgtcac gtagacacgt tgtccttgct ggttatcgta actacgagtc
    24421 tggatacgac ctgtcacccc gataagtgag ccttttttag cccagttagc aagattttca
    24481 gcctgttggc gccacataac gacattaata aaatcagcct cacgttcacc attttgactc
    24541 ttaaatgtac ggtttactgc aagagtaaaa gtcgcaactg ctacatttga tggggtataa
    24601 cgcaactcag cgtcacgtgt catacgccct acaagtacaa cattgttaat catagtttac
    24661 cttcttacgc gtcaattttg acgatcatgt gacgaagaat gtcagcgttg attttggaaa
    24721 gacggtcaaa ctctttaaga gctgcatcgt catttgcttc aacgttaacg atgtggtaaa
    24781 gtccttcacg gaaatcttgg atttcgtatg caagacgacg tttttcccaa gtttttgatt
    24841 caacaacagt tgcaccgttg tcagtcaaaa tagagtcaaa acgtgctacc aaagcgtttt
    24901 tagcttcttc ttcaatgttt ggacgaatga tataaagaat ttcgtattta gccattgata
    24961 tgttcctcct tttggtctaa tgaccccaag actttgcaag gggtaagtga ggttcgctca
    25021 caataaacta ttatactaga aaaaattttt ttacgcaagt aaaaacacta gaattcgaaa
    25081 aaacgccaca tgggcgtttt cctgttctta tggtttgata cggtgcaaca tacgtgggaa
    25141 tggaatagct tcacggatat gttttgttcc tgctgcgaag gttaccatac gttcgatacc
    25201 gataccaaat cctccgtgtg gaactgtacc gtatttacga aggtcaaggt agaattcata
    25261 ttctgtacga tccatgccaa gttcatccat cttagcgaca agggcatcgt aatcttcctc
    25321 acgcatagac ccaccgataa tttctccata gccttctgga gcaagcaagt ctgcacaaag
    25381 cacgcgctct ggatttccag gaactggttt catgtagaag gccttgatgg ctgctggata
    25441 gttcatgaca aatgttggca caccaaagtg gtttgaaatc caagtttcgt gtggtgaccc
    25501 aaagtcatca ccatgctcaa gatgctcgta gtcagcatct tcatcatttt catgctcttg
    25561 caagaggtca atggcttgat cgtaagtgat acgtttgaat ggctctgcaa tgtagcgttt
    25621 caagagttct gtatcacgtt ccaaggtttc caaggcttga ggcgcgcggt caagaacacc
    25681 ttgtagaaga gctttcacat aagcttcttg caagtcaagc gactcatcat gtgtcaagta
    25741 tgagtactca gcatccatca tccagaactc agtcaagtga cggcgtgttt ttgatttttc
    25801 agcacggaaa actggaccaa agtcaaagac acgaccaaga gccatagccc ctgcttctag
    25861 gtaaagctga cctgattggc tcaagtaggc tggcgttccg aagtagtcag tttcaaagag
    25921 ttctgtagaa tcttctgccg catttcctga aagaattggg ctgtcaaact tcataaaacc
    25981 gttcttgtca aagaactcat aagttgcata gataatagcg ttacggattt gcaacacagc
    26041 tacttgctta cgagagcgta gccacaagtg acggttatcc atcaaaaagt ctgttccgtg
    26101 ttcttttggt gtgattgggt agtcttgaga ttcaccgatc acttcgatgt ctgtgatgtc
    26161 caactcatag ccaaatttag aacgttcgtc ctctttgaca atacctgtca cataaacaga
    26221 cgtttcttgg ctcaagcgtt tgataacatc aaacttctca agtcccactt cttcaccaaa
    26281 tttttcgaca aagtttggtt taaaagccac accttgaaag aaggctgttc catcacgcaa
    26341 ttgtaagaaa gcgatttttc cttttcctga tttgttggca acccaagcgc caatcgtcac
    26401 ttcctgacca acatagtctt ttacgtcaat aatcgttaca cgttttgtca ttatttttcc
    26461 ttttcttttt tattctttat ggcaaaccac ctctatattg ttcccatcca ggtcaatcat
    26521 aaaagcagca tagtaaatcg gatgctcact tcgataacca ggaaccccat tgtctcgccc
    26581 acctgcctct atgccagcct cataacaagc ctgaacttct tccttatttt ctgctaaaaa
    26641 agcaaaatga acaggatctt gtgttccctg agtcagccaa aaatcaccac caggatgagg
    26701 gctgttcggg gatagaaaac taattagaga actagtctta aaagccaatt tatagtccaa
    26761 aggaacgaga aaactcctat aaaatccttc tgaaatttgt aaatccttta ccttaatctc
    26821 aaaatgatca atcattctca ctacccataa atgctttcaa gcgttcgact gcttctttaa
    26881 gcgtgtctag gtctgtcgca tagctgaggc ggacattttc tggtgctcca aatccagctc
    26941 ctgttaccaa ggccacttcg gcttcttcta agataacagt tgtaaagtct gtcacatccg
    27001 tgtagccttt catctccatg gcctttttga catttgggaa gagatagaag gccccttgtg
    27061 gtttgaccac ttcaaatcct ggcacctctg caaggagggg atagatggta ttaagacgtt
    27121 cctcaaaggc ctgacgcatg ctttctacag tatcttgctc acctgataga gcctcaactg
    27181 ctgcatattg ggctactgct gacggattcg aagttgtttg acctgcaatc ttggacatgg
    27241 cagcgataat gtctgcttct ccaacggcat aaccaatccg ccaaccagtc atggcatagg
    27301 ttttagacac accattgatg accactgttt gcttgcgaat cgcttccgat aggctagaaa
    27361 tcggtgtgaa ctcatgacca ttataaacca agcggccata gatatcatct gctaggatga
    27421 gaatatcatt ttctacagcc cagtttccaa ttgccaagag ttcctcacgg gtgtaaatca
    27481 tacctgtggg attagatggc gaattcagca ccaaaacctt ggtcttgtca gtgcgagctg
    27541 cttctaactg ctctacggtc accttaaagt gattgtcttc cttagcagaa acaaagacgg
    27601 gaacgccttc tgccatcttg acctgatctc catagctaac ccagtatggg gttgggatga
    27661 tgacttcatc acctggattg accacagcca taaagaagct atagagagaa tatttggctc
    27721 ccgcagcgac tgtcacttga tttgacgcta cagaatagcc gtaaaagcgc tcaaagtagc
    27781 tattgaccac cgccttaagc tctggcagac ctgaggttac tgtataaaaa gaagcacgcc
    27841 catctcgaat cgatgcaatg gcggcatctt ggatattttt gggagtagtg aaatctggct
    27901 cacccaaggt tagagacaaa atatctctac cctcagcctt cagtgctttg gcacgggctc
    27961 cagcagccaa agtcacactt tcttccattt ctaaaacacg gttggatagt ttcataggcc
    28021 ctccttgttg accaatgctc ctgtttcaaa atctactaga taaaaatcag atcctgactt
    28081 aacttcccag attggcttat cttgataacg gccaaaggtt atcttgtcaa tctcgccagc
    28141 tcccttttcc ttagaaaccg tttctgcttt ttcttgtgaa acaccctgat ttagctgata
    28201 aacgtaaatc ttatggtcat ctttaccaat caggacagca agcgcttctt gctgtttgtt
    28261 acgaccaaga acgctgtaat aagattccaa gccattgtat aaatcaacct gatcagcctg
    28321 ctctaatcct gcatactgct gagctaattt ttctccttca cttttagctg tttgataggg
    28381 tttcatgcta agagaaacca tatacagaaa ggaaccactg ataaccacaa acaaaatcgt
    28441 catccctaga ccatactgcc acagtagatt attttttgct ttgttttgtc tttttttcac
    28501 tcgtctattt taccatctat taagctttat tacaagtgaa tataagaata ctcttcgaaa
    28561 atctcttcaa accacgtcag ctttatctgc aacctcaaag ctgtgctttg agcaaccaat
    28621 tctatttctc ccttcaaaca aaaccgattt tgaaagtgaa acagttctta ctttttcagt
    28681 cacaaatgat tagagtttcc gaaattttac aattttcaaa taaactctga gcaaatttct
    28741 tgcaagttct tttgttttgt tgtaatatat tttataacaa cgagagagtt ctcgaaattt
    28801 taagaaaaag gagacacatc atgtctaaaa aagtattatt tatcgtcgga tcactacgtc
    28861 aaggttcttt caaccaccaa atggcgctcg aagctgagaa agcacttgct ggtaaagtgg
    28921 aagttagcta ccttgattat tcagcccttc ctctcttcag ccaagatttg gaagttccaa
    28981 cacatccagc tgtagctgct gctcgtgaag cagttctcgt tgcggatgct atctggattt
    29041 tctctccagt ctacaacttc tctatccctg gtacagtgaa aaacttgctt gactggctat
    29101 ctcgtgccct tgacttgtct gatacacgtg gcgtttctgc ccttcaagac aagtttgtca
    29161 cagtatcatc tgtagccaat gcagggcacg atcaactttt cgctatctac aaagacctct
    29221 tgccatttat ccgtacacaa ggcgttggtg atttcactgc tgcacgtgtt aatgactctg
    29281 cctgggcaga cggaaaattg gttcttgaag aaacagtcct aaactcactt gaaaaacaag
    29341 ctcaagactt ggtcgaagcg attcagtaaa aaatagaaaa ttttagaaag tctaaatcaa
    29401 ttatagccac ctaatagggt ggctttttat ttctcaatca agattttcag acaaacatca
    29461 gccatccaaa aaaaaccttg agcgttacca ctcaaggttt ttagcagtct tttccatcat
    29521 aggaaaatca ttctaagaat attctttcat tcgagatcta tcacttatca tcttctttcg
    29581 agaaattagt gactgctttt ggctgattaa tcttcttttc ttcgtcctac atataggctc
    29641 aatagaatac cgctacctaa aagaacagag atataatcag attggcttcc agtttcaggt
    29701 aatttgtccc cacggttttc tttacctgta gttggtgcta ctgggttagc atttactgga
    29761 gttgctggtt gagccggtgt tggtactact ggttgttctg gaaccgctgg tactggttta
    29821 accggtactt ctggtgttga aacagctgga gttggttgta ctggcacttc tggtgtcggt
    29881 acatttggtg ttggttgcgc tggcacaggt ttaaccggta cttctggtgt cggcacattt
    29941 ggtgttggag gaactggttt gtcttggcat ccacattttc catctttacc gtctttaatc
    30001 accatttctg tcttacttcc atctggatta gtgattgtaa caacgtgagt tccatcgtta
    30061 ttatttttaa cagttacagt tggtgaaacg ctatctttgc cgtctttacc atctttgata
    30121 atagatttgt attcacgacc atcagaatct acgattgtta atgtatgagt tccatctccg
    30181 ttatcaacga tgttcgcttt tggtgattta ccatcaaaga ttgtagttgt cgttccttta
    30241 ccatcagcac ctgtcacttt aatagtgtgt gaaccatcgt tgttatcaat aacttcaagt
    30301 tttggttctc taccatcacg aactgtagtt gttgtagttg ttccgtctga gttcacaact
    30361 gtgatagtgt gagttccatc tccattatcc acaaccttag cagttagtga tttaccgtca
    30421 cgaactgtgg tttcagaaac agtaccatta ccgttttcca cacgaatagt gtaagtacca
    30481 tcttggttat ctctaactgt tgctactggt gatttaccat ctttaatgat tgtttcagta
    30541 gttgttccgt caccatttag aacagagatc ttatgagttc cattttgttc atcagttata
    30601 gtcacttttg gtgatttacc atcacgaact gtggtttcag ttgtcacacc ttctggattt
    30661 gtaataacga ttgtatgact tccgtctcca ttatcacgaa cagatgcggt tggagtacgt
    30721 ccgtcaacac cgtcacgtcc attcttaaca acaaactcat tcttagttcc gtctgggtta
    30781 gtgattgtta ctgtgtggct tccatctgga ttttcagtag ttgttgctgt tgcagcttta
    30841 ccgtctttac catctttgat gatagcgttg gtaacattgc cgtctccatc tgtaacttta
    30901 acagtatgag ttccatcgtt gttatccgtt acttcaactt taggagtctt accgtcttta
    30961 ccgtttttaa caacagtttc tttagttgaa ccatctggat ttgtcaccgt aattgtgtgg
    31021 cttccatctg ggttttctgt tgtagtgata ttcgcagttt taccgtcttt accatcttta
    31081 acaactgttg ttgattcaga accatctggg ttttgaactg tgatagtgtg agttccatct
    31141 ccattatctt ttgtagtaat agttggagct gaaccatcac gtccatctat accgttataa
    31201 atatctttac tatttaatac tttatcgaca ccttcgtcgt ataccccatt gtcattaaca
    31261 tcatcataaa ctgtaacacg gataccgtta cgcgttggag ttgcagcagc atctgtatcg
    31321 cgtctaattc ttgaaggtga agtaatatct tctactttga ttttcggtgc acggccatct
    31381 ttaccattct taccatcttt tacagtgaag gttactggat ctttacctga tgttgtgata
    31441 gttacaacag tgctttggcc atcttgtcca cgttgagtag tgattgtagg agtttgtcca
    31501 tcctgaccgg ttgcaccagt ttctccctta gcaccatctt gaacaaagac tgtagttggt
    31561 tgtgaaccat catggttatc aacagtgatt tctacacctt tatgccctgc ttcatcttta
    31621 cgaacagctg taactgtagg tgattttcca tctactccat ctctaattac cgcaagtggt
    31681 ttttctggat ttaatggttg tcctgggtta gctggatctt cttggtaaac tttagtttct
    31741 ttaccatcct tgacagtaag caatgattta ccatctcgtc cgtccgcgcc tttagcacca
    31801 tctttaatca tttctgaacc tagaagttca tcagttcctg gtgtatattt gccgtcattg
    31861 ttttcatctg tatagaatgt cacaattgta tgagatggat ttccgttttt tccttggaca
    31921 cgaagtaaat caacttttgg agttttaccg ttcagaccat tttctccgtc cttaacattt
    31981 actgtaactg gatctttacc tggaacagtg aatgtaatgt cagtactatg gccatcttta
    32041 cctggagtta cagttacttc tggagtctta ccatcttctc cgcgttcacc tttgtcaccc
    32101 ttagagcctt tgatgttgcc ctctttatcc cagttgccgt tattcttaac gaagacgtcg
    32161 cctgtttctg tattaacgta tttatcgccg tctttacctt gatttgcttc tgggttaact
    32221 ttgccgttca acacgtcttt accgtcacgt ccatcacgtc catcacgtcc gtcacgacct
    32281 gctgctccat ccgcaccttt agctccttgc gctccagtca ggccgcgttc gcctcggtct
    32341 cctttttcgc ctttagcacc atctgcacct ttgtcacctt tagggccttt gatgttgccc
    32401 tctttatccc agttgccgtt attcttaacg aagacgtcgc ctgtttctgt attaacgtat
    32461 ttatcgccgt ctttaccttg atgtgcttct gggttagctt tgccgttcaa cacgtcttta
    32521 ccgtcacgtc catcacgacc tactgcgcca tccgcacctt tagctccttg cgctccagtt
    32581 aggccgcgtt cgccttggtc tcctttttcg cctttagcac catctgcacc tttgtcacct
    32641 ttagggcctt tgatgttgcc ttctttatcc cagtttccat tgtttttaac atagacatca
    32701 ccggtttcgg tattaatata tttatctcca tttttacctt ggtttggttg tggatttact
    32761 tttccgttta atacatcttt accatcacga cctgcttgtc ctgtagctcc atcacggatc
    32821 gtagttgtca catctggact tcctggatta tgaaccgtta atgtatgtgt gccgttttgc
    32881 ccctcagtta ttgttactgt tggagttttc ccatcagcac ccttttgtcc atcctctaca
    32941 aatgttgttt gtggacgacc ttctactggt gtaattgtta ctaatacacc atttttgcca
    33001 ttttgcacac ttcgtgtcgc tgtaattgtt ggtgattgtc cattagtacc gtttcttcca
    33061 tcagtgatag ttgctaatgg ttggtttgga ttaagtggtt gtcctggtct agccggatcc
    33121 tctacataga cttttgtttc agttccattt ttaacagcaa ttagagattt tccatcttta
    33181 cctggagttc ctggattacc ttgttctcct tttggtcctt gtggtcccgc tggtcctctt
    33241 tgtccatctg ctccttttgg tcctgctggt ccttgtgccc cagttgcacc tgttggccct
    33301 ctttctccat ctttaatatc tgtatttaaa attacttcat cttgattatt aaagacacca
    33361 tcaccatctt tatctaaata aaatttaatg tgattcgtat cccctgtttt attttgagtt
    33421 gcagcaattt taccaatacc gtatttcatt acacgaggtt tagcagctgt aactgtagta
    33481 gcattcccta caggaagtcc taaaacatac tgatctccaa atttatgaag tctgtatgct
    33541 tgtgatttct ctccttctac cccttcagaa actaactccg ttactccttc tgctaattta
    33601 gtagttggta tatataaagt ttggaaatca actttagtat taaaacttgt tgttaaagga
    33661 ttagcttgat ttggatttgc atctagatac aatggatccg tttcaaaata atagttagaa
    33721 ttactatatg gatcattact agtcccagtt ccactaccac cttggtctac gttaaattca
    33781 actggacgta aacgaagaga taaatctccg ttagggttag ttataggtaa actaaacgta
    33841 attgtttgac ttttgtttgt ttctactgca gcttctaaac tgtagcctga atgagaaaca
    33901 aagtttccac ccttactaat gtttttaact tggtttctta agtttggtgt tataacagta
    33961 gcaggtgtag gttcaccata acctgttccc ttatctaaga taaagttgtt attaacaaac
    34021 tctctaagat ttcctggcgc aaaataatta attgtaaagt taatattatt gcctctacgt
    34081 actgtcgtta catcataatg gatttcacga ccaccacgtg aatgtcctat accacgaaat
    34141 gaatgtgagc cctgactgat gtcagaaaat gcaatagtat tatttggatt taagattggt
    34201 ttattttctg gtccagctgc tctaaaacca cttcctgctg ttctaaaacc actatacccc
    34261 ctatcaacat cttccttacg gatagctgga ttttggcgag catcagaaag gctcaaacct
    34321 ggtttcaaga tttcattagt agatgctgga gctgaagttg tttctacttt agcagctgtg
    34381 ctagttggca aaacagcgct atcaacagct gaacgatcag ttgttgcagc tggattagtc
    34441 tcttccttcg cttcaactat attagctttc tttgctacag gcgtttctga tacagttcct
    34501 ttgttagagt tagaacgaag ttctagacca ataatagatg attgaataga tgactctgct
    34561 gctgataatt cagcttcaga tgcatctgct ttcccaagca agctttgacc agcttggata
    34621 cttgtttgca agttagcagc acttgcttct gtatataact tgctttctaa ctttttagca
    34681 cgcgaaagag ccgttaacaa ttttgacttg tcaatagcat cttgatgcgt tacttcctct
    34741 tttttgtctt ttgcttcttc aactttttct gctggtttag taacttctcc agttttaact
    34801 ggagtcgttt ctactggatt agctacagtt ggagctgcat aggttttctc tttattagac
    34861 gcagaaacct ctgctggttt agtagctgct tctcccctac caacagaaat actattggta
    34921 gtttcttgtt tgttttcttc cgcttgtact gtttggacac tagctgctgc acctaatacc
    34981 aaagcggtac cgagcaagac actaactgca ccaaagtaat acttacgaat agaataacgt
    35041 tgttgcatgc tataccagtc aaatgatttt ttatgtgaat gtttcatttt aaactcctag
    35101 atagtatttt atagtttaca atgtacaact atttgagaca ttaatcaata ttttctgaac
    35161 aattaatata ttctgttttt gcacttaaaa gccacttata ttattctaat ttttccgatt
    35221 actaacgaca attttcttgc tctaattttt ttatactact tagacaaaaa aagatataat
    35281 gaattatgga ggaaataccc gtatgagaaa ccttttatcc acaaaagttc aaagacaatt
    35341 acgcttaatg gaaaccctaa ttcagaatcg taattggatg aaattacatg aactagccga
    35401 aaaattagga tgtacagaac gaatcttaaa aagtgattta aatgaattac gtattgcctt
    35461 tccatctatt aacatccaat cttctgttaa cggaatcatg atagatttag aagttaacac
    35521 tagtgtagaa gatatttatc aatattttct tgctaattct caatcttttc aattactaga
    35581 gtatatgttc ttcaatgagg ggctacctat ttatcgaaca atagaaaatc tttactttag
    35641 tagtgccaac ctctatcgac tgggcagaaa tattacaaag gttctttcat ctcagtttca
    35701 aattgaatta tcttttactc catccgagat acgtggaaat gagatcgata ttcgttactt
    35761 ttttgcccaa tacttttctg agcgctatca tttcctagat tggccttttc ctgaccttcc
    35821 tgaggaagat ttaacagagt ttgctgactt cttctataaa atcacgaatt atccaatgcg
    35881 attttcaatt tatagaatgt acaaactgat gatagctatc agtatccaca gggttaaaaa
    35941 tggtcacttc attgacctcc ccaaccactt ttacaaagaa tattatcctc ttttaaagag
    36001 cattccaaat ttccaggaga cacttgctta tttctctaaa cattttgggc ttgaaatgac
    36061 tccagataca atcgctcaaa tttttatttc ttttcttcag aatgatattt tcctagatcc
    36121 tcaagaattc ttcaactctt tagaagataa cagtcaagca agatactctt accaattact
    36181 tagtcaaata ttagaacgcc tatcaaaaca atataagatt acttttacta accacgatga
    36241 gctgatttgg catttacaca acactgcttt ctttgaaaga caggaaatct tttctacccc
    36301 aatcttattt gaacaaaaag cattgacgat taaaaaattt gaagtttact tcccagactt
    36361 tatgggaagt gcgcgtcaag aactggctca ataccgtcag gcaattggac agcatgacca
    36421 tcctgaacag ttggaacact tgatgtacac catcttaacc catgctgaaa acctctctac
    36481 tcagttgtta gaaaatcgac cacctatcaa ggttttgatt atcagtaact ttgaccatgc
    36541 tatatccctt acctttgttg atatgctttc ctattactgt aataaccgct ttaccttcga
    36601 tatttgggat gaattaaaaa caagtcctga gattttaaat cagacagatt acgatatcat
    36661 cgtgtctaat ttctatattt cagggattac caagaagttt atttgccgaa atcatctgtc
    36721 aatcatgaat ttggttaatc atcttaacac cttatcaaat gagattcatt tgtccaatac
    36781 tttataagtt atcaaaaaag tcagcataat cgtgctgact tttctcttat ctgatttcta
    36841 tacttccttt acaaaaacca atccctgtgg ttgtgacaaa tcttctcggt aaacaaatcc
    36901 agcaaagttc aagcgacgtg cttcccccac agtttgtact tgattttcta ttaaagctgt
    36961 tagaaaggcc ccgcgcgctt tcttggagat agttgagtga atcttcagct gaccgcctct
    37021 atcctccatg aatttgaagg tcaccatctt tgctctgatt tccttagaaa atacagtctc
    37081 aaactctgat gacaagagag agaaaatcac ttcttccttc ttcagagctt catcatagac
    37141 tgccttccaa tggctcttca aagtcttacc agcgactttt aatttcatca aaaaatccaa
    37201 acggtgagga gccatgggtg acaaggctgg aacaacaccg tacaaagccg aggtaatgaa
    37261 gacatgattt tcaagataat cttgttccgc ctcggtcagc ttatctctct taatgttgcg
    37321 gtacataagc ccatcaaaaa gtttcaaggc tggatagtgt tgagcagttt gccttttcaa
    37381 agcttggata ttttgaaatt cttccgccgc tttctcagct gataccttgt agaaactctc
    37441 caattgactg gcagaataga gagccaaggc atcaagcacg gtctgacttt ctggttttaa
    37501 aggaattgcc tcgatacttg gcaagtctgt gttcatttct tttgctgttg ggattaaaat
    37561 tttcatattg atagtgtagc atatttttta gccacaacca agaaattcat ttgaaaa
//
LOCUS       DAVKWZ010000013        37555 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000013 DAVKWZ010000000
VERSION     DAVKWZ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37555
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.013"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(63..872)
                     /locus_tag="VW854_000729"
     CDS             complement(63..872)
                     /locus_tag="VW854_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV0262003.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGIGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
     gene            complement(874..1677)
                     /locus_tag="VW854_000730"
     CDS             complement(874..1677)
                     /locus_tag="VW854_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV0262004.1"
                     /translation="MKQLTVEDAKQIELEILNYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPIDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            complement(1687..3174)
                     /gene="tacF"
                     /locus_tag="VW854_000731"
     CDS             complement(1687..3174)
                     /gene="tacF"
                     /locus_tag="VW854_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEV0262005.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVAPEYYLLTTML
                     SEASLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            3510..4217
                     /locus_tag="VW854_000732"
     CDS             3510..4217
                     /locus_tag="VW854_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEV0262006.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKNIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            4222..5244
                     /locus_tag="VW854_000733"
     CDS             4222..5244
                     /locus_tag="VW854_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV0262007.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPEAEIVVIGRHWEKLELFSFAKECYITDNI
                     PEDLAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFENAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            5261..6130
                     /gene="licA"
                     /locus_tag="VW854_000734"
     CDS             5261..6130
                     /gene="licA"
                     /locus_tag="VW854_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEV0262008.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKITPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     IKGLASYGGSDEK"
     gene            6120..6998
                     /locus_tag="VW854_000735"
     CDS             6120..6998
                     /locus_tag="VW854_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV0262009.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAATHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFVAVANGQL
                     LGLMIVFVAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAALFLGTSLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            7010..7699
                     /locus_tag="VW854_000736"
     CDS             7010..7699
                     /locus_tag="VW854_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEV0262010.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            7785..8633
                     /gene="dprA"
                     /locus_tag="VW854_000737"
     CDS             7785..8633
                     /gene="dprA"
                     /locus_tag="VW854_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEV0262011.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLALSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            9516..10559
                     /locus_tag="VW854_000738"
     CDS             9516..10559
                     /locus_tag="VW854_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HEV0262012.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            10726..12813
                     /gene="topA"
                     /locus_tag="VW854_000739"
     CDS             10726..12813
                     /gene="topA"
                     /locus_tag="VW854_000739"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEV0262013.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQNGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            13034..13393
                     /locus_tag="VW854_000740"
     CDS             13034..13393
                     /locus_tag="VW854_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEV0262014.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            13486..14118
                     /locus_tag="VW854_000741"
     CDS             13486..14118
                     /locus_tag="VW854_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEV0262015.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYSDLEIAIMLEDIRLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLENRQTFIDQVGVTQLHGTKVVF"
     gene            14162..14818
                     /locus_tag="VW854_000742"
     CDS             14162..14818
                     /locus_tag="VW854_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEV0262016.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVHMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            14837..15871
                     /locus_tag="VW854_000743"
     CDS             14837..15871
                     /locus_tag="VW854_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEV0262017.1"
                     /translation="MNFQLAKYSLLKKFSENIEFTTPGEYGAIFKYLIENVKTDRQII
                     CSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAGNAALEEIAVALNIRQDYYQ
                     VETSIVLNETINTSEMVSRFSGIHQDGVLKNPLTYEIITPELVGVKIPLGKLSGRHAF
                     VEKLRELALDFTEEDIKPLFAKFKALADKKQEITDADTRALVAGTMVENPEGFHFDDL
                     QLQTHADNDIEALVSLANMDGEKVEFNATGQGSVEAIFNAIDKFFNQSVRLVSYTIDA
                     VTDGIDAQDRVLVTVENRDIETIFNAAGLDFDVLKASAIAYINANTFVQKENAGEMGC
                     SVSYHDMPSV"
     gene            15883..16920
                     /gene="leuB"
                     /locus_tag="VW854_000744"
     CDS             15883..16920
                     /gene="leuB"
                     /locus_tag="VW854_000744"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEV0262018.1"
                     /translation="MAKKIVALVGDGIGPEIMEAGLEVLETLAEKTGFDYEIDRRPFG
                     GADIDAAGPPLPDETLKASREADAILLVAIGSPQYDGVAVRPEQGLMALRKELNLYAN
                     IRPVKIFDSLKYLSPLKPERISGVDFVVVRELTGEIYFGDHILEERKARDINDYSYEE
                     VERIIRKAFEIARNRRKIVTSIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSA
                     AMLMITNPAKFDVIVTENLFGDILSDESSVLSGTLGVMPSASHSENGPSLYEPIHGSA
                     PDIAGQGIANPISMILSVVMMLRDSFGRYEDTERIKRAVETSLAAGILTRDIGGQAST
                     KEMMEAIIARL"
     gene            16917..17186
                     /locus_tag="VW854_000745"
     CDS             16917..17186
                     /locus_tag="VW854_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEV0262019.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRAWRIQEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            17190..17549
                     /locus_tag="VW854_000746"
     CDS             17190..17549
                     /locus_tag="VW854_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEV0262020.1"
                     /translation="MAGSSREHAAWALANYGFKVVIAGTFGDIHYNNELNNGLLPIVQ
                     PREVREKLAQLKPTDHVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            17711..18335
                     /locus_tag="VW854_000747"
                     /pseudo
     CDS             17711..18335
                     /locus_tag="VW854_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007122522.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
     gene            18498..20181
                     /locus_tag="VW854_000748"
                     /pseudo
     CDS             18498..20181
                     /locus_tag="VW854_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042409.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            20356..21342
                     /locus_tag="VW854_000749"
     CDS             20356..21342
                     /locus_tag="VW854_000749"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEV0262021.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEVGRISFIKRMHDQGLIASISVGVKDYEY
                     DFVSQLKADAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            21406..21477
                     /locus_tag="VW854_000750"
     tRNA            21406..21477
                     /locus_tag="VW854_000750"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21439..21441,aa:Arg,seq:ccg)
     gene            21660..22358
                     /locus_tag="VW854_000751"
     CDS             21660..22358
                     /locus_tag="VW854_000751"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEV0262022.1"
                     /translation="MKELQTVLKNHFAIEFTDKKLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            22349..25888
                     /gene="smc"
                     /locus_tag="VW854_000752"
     CDS             22349..25888
                     /gene="smc"
                     /locus_tag="VW854_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEV0262023.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLATLEDKRSS
                     LSQEKSDKESSLALLEENLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSHQLSQKQADQLEKLKEQLATAKEKAGQQKAELETAKEQVQK
                     LLADYQVCAKEQEEQKTSYQAQQSQLFDRLDSLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDMHYQTALEIALGASSQHIIVEDENAATKAIDLLKR
                     NRSGRATFLPLTTIKARTISSQNQDTIAASPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAEEEASLRSDEVSLKNLQDELARLTERLEAIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEELNRLSEGEWQADKEKCQERLTTITSEKNNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLDIEQEEIQRLLQEKVDN
                     LEKVDTDLLSQQADEAKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQNQLTDQYQISYTEALEKAHELENLNLAEQEVQDLEK
                     AIRSLGPVNLDAIEQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            25885..26679
                     /locus_tag="VW854_000753"
     CDS             25885..26679
                     /locus_tag="VW854_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV0262024.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFAGVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNDNVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKERSVIAYMEGL"
     gene            26679..27497
                     /locus_tag="VW854_000754"
     CDS             26679..27497
                     /locus_tag="VW854_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV0262025.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            27501..28778
                     /gene="ftsY"
                     /locus_tag="VW854_000755"
     CDS             27501..28778
                     /gene="ftsY"
                     /locus_tag="VW854_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEV0262026.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAEVEIVEQAVFQEEEIQDTVEESLDLEPVIEVSQKEVKEFPHSEEGNTEFLETVE
                     ENNSEILEAERPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEELEEL
                     LIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDESIHFQ
                     DNLTVMLFVGVNGVGKTTSIGKLAHRYKRAGKKVMLVAADTFRAGAVAQLAEWGRRVD
                     VPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGRIIKR
                     VVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAIREEL
                     NIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(28824..30311)
                     /locus_tag="VW854_000756"
     CDS             complement(28824..30311)
                     /locus_tag="VW854_000756"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEV0262027.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRSVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEELWD
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDIAYRQDGRLE"
     gene            complement(30460..31200)
                     /locus_tag="VW854_000757"
     CDS             complement(30460..31200)
                     /locus_tag="VW854_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV0262028.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(31200..33365)
                     /locus_tag="VW854_000758"
     CDS             complement(31200..33365)
                     /locus_tag="VW854_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV0262029.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALAEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            33538..34491
                     /locus_tag="VW854_000759"
     CDS             33538..34491
                     /locus_tag="VW854_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0262030.1"
                     /translation="MKNKRIFKDFQASKMNLNIYTSPLLAFVFVFIGEFVAYTLYGIS
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            34568..36556
                     /gene="uvrB"
                     /locus_tag="VW854_000760"
     CDS             34568..36556
                     /gene="uvrB"
                     /locus_tag="VW854_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEV0262031.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTIGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            36566..37129
                     /locus_tag="VW854_000761"
     CDS             36566..37129
                     /locus_tag="VW854_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0262032.1"
                     /translation="MIYLRKLKREDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKQTRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            37116..>37555
                     /locus_tag="VW854_000762"
     CDS             37116..>37555
                     /locus_tag="VW854_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0262033.1"
                     /translation="METPKEIEGKSLVHWQMWREAYDDLLPAEFQETMTLERCRLFSQ
                     KYPENTLIAIGDLKVVGFISYGNFRDETMQAGEIIALYVLKDYYGKGIAQKLMKAALT
                     ALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIK"
BASE COUNT        11659 a         6552 c         8201 g        11143 t
ORIGIN      
        1 tttctactaa aattatgttt ttgaataacc tctatcttag tagtttgtat aatccccctc
       61 aatcagtttt tgcgataagc tttaatgcta tgactatacc actcttgcat ttcttttgat
      121 ggtggtgtca tataatcgcc atacatctgg gttaaaaatt ggtcatattt tttgggaaca
      181 ggcaacatac ggccctcaaa ctcagttaaa atcagttctt taaaggtatc aactgggaag
      241 atttctttca tcccttcctt accgatccca attcctcctt catattgagg agtgttggtt
      301 acagcatttt tgactagttg atcaattttc ttgtaaaagt agcgaggatt gacaaatcgg
      361 agagcgtacc agctacataa tctaagaaaa tcttttagtt tgctatcacc gtgaactgct
      421 cgtgattttt tgatataagc tagttgacga agagccacat acttatagct cttgtcgaca
      481 atactcaagt ctgtaaatcg atcaattggg aagacatcga tgaaaaggct ggtatcatga
      541 cgcttgtact taacatggtc ttctataaca gtagaagtgt ccaaaatcga tgcgaaatta
      601 tggaagtacc aagaagatgt atcgtaggaa agaaccttgt agcgagggtg attttcttct
      661 tcaataatct tcagtaaacg ctcataatcc tcacgataaa gggaaatatc aatatcatca
      721 tcccaaggaa tcataccttt gtggcggatg gctccaagca tggttccata actgagaaaa
      781 taaggaatat catgtttctt acaagtctca tcaatatagt ccagcagggc tagttgaatt
      841 tctttaattt cttttttttc taaatattgc atcctaatcc tccaatttat aagcgtgaaa
      901 ttcatgactg tagaagcgtt tttcttctgg tggtagggtc atataatctc cataaaattg
      961 tgtcaaaata gtatcaaatt tttcaggtgc aggaaggctt aaattctcaa agggtaaatc
     1021 gattgtttta tcaaaggtac cacttgggaa gacttccttt tccttaaatt ttgaagggat
     1081 aaaagccata tattgcccat tttcacgact atatttttga atttctttct cgattttatt
     1141 tgcaaaataa cgaggagaaa ccggtcgaag gagtaaccag aaggctgttc gtatccaatc
     1201 ttttaaaagg ctatccttat agacaatatt tttatgttta ctgaaagaca gcagtttgaa
     1261 gctttccagt ttataacaag tatcaatgac cttaggatca tcaaagcgat ctatagggaa
     1321 aatatcgata aagataccag actcataggt ttttgtattt cgagtatcaa taattttagt
     1381 cgtactgtcg gttatcttga taaagttgtt aaagtagttc ttatcagttt ctaaggatag
     1441 gagcttatac ttgctttttt ccttttgaaa aatgttaata aatcgttggt agtcttctct
     1501 aggcatggat aaatcaatat cgtcgtccca agggataaag ccctcatgtc gaaccgcccc
     1561 aatcagagtg ccgtagttaa taatatagtt gatattgtgc tttttacaga gagtatcaat
     1621 ataattcaaa atttctaatt caatttgttt ggcatcttca acggttagtt gtttcatttt
     1681 aaactcctaa gattttttaa atttattttt taaggctagg acatggttta aaaattcata
     1741 gaaaatgcta tcttttgtga agacaagtag actaatataa gagatagctg atagcaagac
     1801 aatcaaacca gtattaatca aaaatggcaa attaatgacc atatccacag gatacacgaa
     1861 attaatcagg aaataaattg ctacaaagga aagtgaaaag agagagtatc gaacagtata
     1921 gctaaagata tgtcccaagt ggatgagttg ttttctatgg atgaaaatga tatagaaaac
     1981 aagtagagaa gcctctgata acatagttgt cagtaagtag tattcgggag ccacgatatg
     2041 gttgaaaaag aggagactat tcaagcccaa attgagtagc ccagcaaaga ctgtatagac
     2101 tgtgatacgt ttttcatagc cattggtaaa gagaatttgg gaaccaagaa tggtatctaa
     2161 ggccagaata atcgtacgaa aagcgaagag agaggtcaag atgccgcctc cgatatattt
     2221 ttcactaccg taaagtagga tggcatttgg tcctaaaacc atgagtccaa aactcagtgg
     2281 aatgataaag aagttaaaga ttcgactacc tctattaacc agagaaacat aggcttcttt
     2341 gtctcctttc cccagatagt aactgagacg aggcacactc actccaattg cacctgttac
     2401 aaccccagct ataacggtca caattcgctg agctatggta tagtaactaa cgttgacatc
     2461 aatccctgtt ttaacgagga agaggcgatc taaaaaagtg aagagcatat tggcattggc
     2521 aaagactaac atggctgtca gagggagaaa gagtggttta aaatcactta ggtgaatctt
     2581 aacaagtttg atgtctcttt taatccaaaa ataactaatc aggtagttaa tcagcgtcga
     2641 taaactcatc acaagtgtat agacaacaat atcgtgttca tttttaacaa ataagaaaat
     2701 agagaccagc atcaggatac ggatgaaggc agttttgtaa aagagaaaac tgtaattttc
     2761 cagagcttca ttgacccatt cgattgaaaa aatctgggca atgagttgaa tccccataac
     2821 aaggtagacc tttttgacga ttggattatc agtaaagaag agaggatagg ctaggatata
     2881 gacagcagtg gtcaaaatcg tacaagcgat gcacaaataa aaaagactag aaaaggttct
     2941 gttaagatct tttttgttat ccttgacatt actgatagcc cttaaaccgt agttatagac
     3001 accataagtt gcaaagggca agaaaaatga caaaatagtg tcgactgagt tgaagtaacc
     3061 atagtcagtt cggtccaaga cacgcgcgac ataggttcca gttaggatgg gaaaaataat
     3121 attcaagaca cgaattccca tgtaagatag agcatttaat tttatacttt tcattcaatt
     3181 tacctcgttt ttcattatat cataaagtta gctaataaga aatgaagggc agtaagtcaa
     3241 gtaatcactt tgaagtttca aatcttaagt tttaagtttt ctttaaggaa agtatattat
     3301 tctgaaggac tctaaaattt cgcagccatt tattagtaat tgctacagaa ttcctagtca
     3361 ttactagaaa tggactagtt tcttggaata atagaactgc ataattctcc tattctagaa
     3421 ggggaggacc agtatttctt ttatgatagg actagattgt ggtataatag agagaataag
     3481 tttttttagt aagacaaagg agaaaataga tgatttatgc aggaattctt gccggtggaa
     3541 ctggcacacg catggggatc agtaacttgc caaaacaatt tttagagcta ggtgatcgac
     3601 ctattttgat tcatacaatt gaaaaatttg tcttggagcc aagtattgaa aaaattgtag
     3661 ttggtgttca tggagactgg gtttctcatg cagaagatct tgtagataaa tatcttcctc
     3721 tttataagga acgtatcatc attacaaagg gtggtgctga ccgcaataca agtattaaga
     3781 acatcattga agccattgat gcttatcgtc cgcttactcc agaggatatc gttgttaccc
     3841 acgattctgt tcgtccattt attacacttc gcatgattca ggacaatatc caacttgccc
     3901 aaaatcatga cgcagtggac acagtggtag aagcggttga tactatcgtt gaaagtacca
     3961 atggtcaatt tattacagat attccaaatc gtgctcacct ttatcaagga caaacacctc
     4021 aaacattccg ttgcaaggac ttcatggacc tttatggatc tctttctgat gaagagaagg
     4081 aaatcttgac agatgcatgt aaaatctttg tgatcaaagg aaaagatgtg gctttggcca
     4141 aaggtgaata ctcaaatctg aagattacaa ccgtaacaga tttgaagatt gcaaaaagta
     4201 tgattgagaa agactagtaa aatgattaat caaatttatc aactaactaa gcctaagttt
     4261 atcaatgtca aatatcagga agaggctatt gaccaagaga atcatatcct tatccgtccc
     4321 aactacatgg ctgtctgtca tgcggatcag cgttactatc agggaaaacg tgatcccaag
     4381 attttgaata aaaagcttcc aatggcaatg attcacgagt catgtggaac cgtcatttct
     4441 gacccgaccg gaacctacga ggttggtcaa aaagttgtca tgattcccaa tcagtctcct
     4501 atgcagagtg atgaagaatt ctatgaaaac tacatgacag ggacccattt cttgtctagt
     4561 ggatttgatg gctttatgag agagtttgtt tctctcccta aagatcgtgt ggtggcttat
     4621 gatgctattg aagatacggt tgcagccatt acagagtttg tcagtgtggg catgcacgct
     4681 atgaatcgtc tattgactct tgctcatagc aagcgggagc ggatcgccgt tattggagat
     4741 ggaagtttag cttttgtggt tgccaatatt atcaactata ctttgccaga agcagagatt
     4801 gtggttattg gtcgtcattg ggaaaagttg gaactcttct catttgccaa agaatgctat
     4861 attacggata atattcctga agatttggcc tttgaccatg cttttgaatg ttgtggtggt
     4921 gatggtactg gaccagctat taatgacttg attcgctaca ttcgtcctca gggaacgatt
     4981 ctcatgatgg gagttagcga atataaagtc aatctcaata ctcgcgatgc cttagaaaag
     5041 ggcttgattt tggttgggtc atctcgttct ggtcgcattg attttgaaaa tgctatccaa
     5101 atgatggaag tcaagaaatt tgccaatcgt cttaaaaata tcctttatct agaagaacct
     5161 gtaagagaaa ttaaagatat tcatcgtgtc tttgcaaccg atttaaacac agcctttaaa
     5221 acagtgttta agtgggaagt ataagtactg gaggttaatt gtggagaaaa tcattaaaga
     5281 aaaaatttct tccttactta gtcaagaaga ggaagtcctc agtgttgaac aactgggtgg
     5341 aatgaccaat caaaactatt tggccaaaac aacaaataag caatacattg ttaaattctt
     5401 tggtaaaggg acagaaaagc ttatcaatcg acaagatgaa aagtacaatc ttgaactact
     5461 aaaggattta ggcttagatg taaaaaatta tctttttgat attgaagctg gtatcaaagt
     5521 aaatgagtat atcgaatctg cgattacgct tgattcaacg tcaatcaaga ccaagttcga
     5581 caaaattact ccaatattac aaactattca tacgtctgct aaggaattaa gaggagaatt
     5641 tgctcctttt gaagaaatca aaaaatacga atccttgatt gaagaacaaa ttccttatgc
     5701 caactatgaa tctgttagaa atgcagtctt ctccttagag aaaagactgg ctgacttagg
     5761 tgttgacaga aaatcttgtc atatcgattt ggtgcctgaa aactttatcg aatcacctca
     5821 aggacgactt tatttgattg actgggaata ttcatcaatg aatgatccaa tgtgggattt
     5881 ggctgccctc tttttagagt ctgaattcac ttcccaagag gaagaaactt tcttatctca
     5941 ctatgagagt gaccaaacac cggtttctca tgaaaagatt gctatttata aaattttaca
     6001 agatactatt tggagtctat ggactgtcta taaggaagag caaggtgaag attttggtga
     6061 ctatggtgtg aatcgttacc aaagagctat taaaggtttg gcttcttatg gaggttcaga
     6121 tgaaaagtaa aaacggagtt ccttttggcc ttctctcagg tattttctgg ggcttgggtc
     6181 taacggttag tgcttatatc ttttcgattt ttacagattt gtcacccttt gtggtggctg
     6241 caactcatga ttttttgagc atctttatct tactagcttt tctcttggta aaagaaggga
     6301 aagttcgcct ctcaattttc ttaaatattc gcaatgtcag tgttatcata ggagccttgc
     6361 tagcaggccc tatcggtatg caggccaatc tttatgcagt taagtatatc ggaagttctt
     6421 tagcttcatc tgtatcggct atttaccctg cgatttcagt tctattggct ttcttctttt
     6481 tgaagcacaa gatttcgaaa aatactgtat ttgggattgt cttgattatt ggagggatta
     6541 ttgcccagac ctataaggtt gaacaggtta attctttcta cattgggatt ctttgtgctt
     6601 tggtttgtgc tattgcatgg ggaagtgaga gtgttcttag ctcttttgcc atggaaagcg
     6661 aattgagtga aatcgaagcc ctcttaatcc gtcaagtaac ttcgttcttg tcctatcttg
     6721 tgattgtgct cttctctcat cagtcatttg ttgcagtagc caatggacaa ttgctaggtc
     6781 tcatgattgt ctttgtagcc tttgatatga tttcctattt ggcttattat atcgctatca
     6841 atcgcttgca accagccaag gctacaggct tgaacgtgag ctatgtagta tggactgtct
     6901 tgttcgcagc tcttttcttg ggaacatcat tagatatgct gaccattatg acgtcacttg
     6961 tcgtcattgc tggagtttat attattatta aagaataaag gagattcgtg tgaaagccat
     7021 tatcttagca gcgggattgg gaactcgctt gcgtcctatg actgaaaata cccctaaagc
     7081 cttggttcag gttaatcaaa aacctttgat tgaataccaa attgagtttc tcaaagaaaa
     7141 aggaatcaat gacatcatca tcatcgttgg ttatcttaaa gaacaattcg attatttaaa
     7201 agaaaaatac ggtgttcgcc tcgttttcaa tgataaatac gctgactaca ataactttta
     7261 ctctctctat cttgtaaaag aagaattggc caacagctat gttattgatg ctgacaatta
     7321 tctctttaaa aatatgttcc gcaatgattt gacacgttcg acttatttta gtgtttatcg
     7381 tgaagattgt accaacgaat ggttcttggt ctatggagat gactacaagg ttcaagacat
     7441 tattgttgat agcaaggcag gtcgcatcct tagtggtgta tccttctggg atgctccaac
     7501 tgcagaaaag attgtcagct ttatcgacaa ggcttatgca agtggtgaat ttgttgatct
     7561 ctactgggac aatatggtta aggataatat caaagagcta gatgtctatg ttgaagaatt
     7621 agaaggcaat agcatttatg agatcgatag tgtccaagac tatcgtaaat tagaagaaat
     7681 tcttaaaaac gaaaattaaa gattccaaca tctgacaaaa tagtcggatg ttttttgatt
     7741 ttttacgaat agatagatga gtagaaaaag aaatggagtt atttatgaaa atcacaaact
     7801 atgaaatcta taagttaaaa aaatcaggtt tgaccaatca acagattttg aaagtgctag
     7861 aatacggtga aaatgttgat caggagcttt tgttgggtga tattgcagat atctcaggtt
     7921 gccgtaatcc agccgttttt atggaacgtt attttcagat agacgatgcg catttgtcga
     7981 aagagtttca aaaatttcca tctttctcta ttttagatga ctgttatcct tgggatttga
     8041 gtgaaatata tgatgcgcct gtacttttat tttacaaggg aaatcttgac ctcctgaaat
     8101 tcccgaaggt agcggtcgtg ggcagtcgtg cttgtagcaa acagggagct aagtcagttg
     8161 aaaaagtcat tcaaggcttg gaaaatgaac tggttattgt cagtggtctg gccaagggca
     8221 ttgacacagc agctcatatg gcagctcttc agaatggcgg aaaaaccatt gcagtgattg
     8281 gaacaggact ggatgtgttt tatcctaaag ccaacaaacg cttgcaagac tacatcggca
     8341 atgaccatct ggctctaagt gaatatggac ctggcgaaca acctctgaaa tttcattttc
     8401 ctgcccgtaa tcgcatcatt gctggacttt gtcgtggtgt gattgtagca gaggctaaga
     8461 tgcgttcagg tagtctcatt acctgtgagc gagcaatgga agaaggacgc gatgtctttg
     8521 ctattcctgg tagcatttta gatggactat cagacggttg ccatcatttg attcaagaag
     8581 gagcaaaatt ggtcaccagt gggcaagatg ttcttgcgga atttgaattt taaaaatgac
     8641 ctaagctaga attctaagaa aaaatcaatt ttaagagaaa atgaacccaa catttccata
     8701 ataaaacgca tattagcaag tttttaacac ttgataatat gcgttttttc taagtggatt
     8761 agtagagtag aggatttttt catataatac tcttcgaaaa tctcttcaaa ctacgtcagc
     8821 ttccatctgc aacctcaaaa cagtattttg agctgacttc gtcagtctta tctacaacct
     8881 caaagcagtg ctttgagcaa cctgtggcta gcttcctagt ttgcgctttg attttcattg
     8941 agtataaggg aaagtatagt gaattgaaat aagatgtgaa caactctatc aggaaagtca
     9001 aattaattta tagaaatatt ttagcagcca aggtgcactg ttatagattc aatacactat
     9061 aatttagtgt aattgagaaa ggagaaatga ttgtgattga tgttggctag gttatgttca
     9121 atgattccta ccgtctcaaa tcttgtcagt aaggaaaaat aaattcttca aaagtagaga
     9181 ttacaaggct tgtttaagaa agaattcaaa gaccttgaca aataaaaata aaatggttat
     9241 tataagaaat ggtctaaaat agatgatgat acttttcgaa aatcttttta cgtcagctca
     9301 gctttgcctt gctgtgtttt gagcaagcta cggttagctt ccgagtttga ttttcattta
     9361 ctagaaatga aactgatgag agatatcagt agacatttga gtcaggatat tatggaaaat
     9421 gataaaaaga gctcgtgaga ttggcatatc agactactaa agtattgagt ttgttaggat
     9481 tttagcgact agttagctgg gaaaggaaga tatttgtgac aaataataaa ctgtattcgt
     9541 tgatagaatt tagaaataaa atatatgaag aattagaact ttccagaagt gatttagcga
     9601 ttttactatg tgccatgctt atcgcctcta tcggattaaa tatggattcg actcccgtga
     9661 ttattggagc catgttaatc tctcctttga tgacacctat tctgggagtg gggctctctc
     9721 tagctatatt tgattttaaa ttgttaagaa aatcttttaa aatattagct attcaaattc
     9781 ttgccagtct aatagcttca acactttatt tttatctttc tcccatttcg tatgctagtt
     9841 cggagattgt tgctagaacc tctccgacta tttgggatgt tctcattgct tttgtaggag
     9901 ggatagcagg tattattggt gctaggaaaa aagagaccaa taatattgtt cctggtgttg
     9961 ctattgcaac cgccttgatg cctcctcttt gtacagtagg ttatgctatt gcttctgcta
    10021 atctaaaatt tatcataggc tcctcttacc tattcctcat caattgtagc tttattgtca
    10081 ttgcgactta tataggtgtt aggttgatga tggttaagaa acattatttt aaagataatg
    10141 aagaagactc taaaatgcgt aggattttgc ttctagttgc tgttttgctg atgattccga
    10201 gtttcatctc tgcaacgact ttagtgagag aaacgttgaa aaaagagtcc cttaagaaat
    10261 ttatatcaga gcagtttcag gggtataata ttttgaaaaa aacctattct aaaaagactc
    10321 ataccctaaa gctaaccatt tcaggaaatt atttgacaga agaagaactc gatatgattt
    10381 caagtaagag aggtgactat ggtttaagtg atgtttctgt tcaagtttca caattgtctg
    10441 attcagaaca acttagtaag gaagagctgg tggagtattt cttccagtat atcaaggata
    10501 aggaagcaaa agaaaaggag aaagccaata aatttgatac agagtctgag gagcaataat
    10561 ttcttgagaa tagctggttt ctctcgtgag tcttctatgt atatcaaagg aagactgagg
    10621 ttttaagtat aaaacttttc ttctattata gtagctaagt cttgacaggg ttcaaaaaat
    10681 ggtttacact ttataaagtt tattactttg aaaaggtgtg atactgtggc tacggcaaca
    10741 aaaaagaaaa aatcaacagt taaaaaaaat ctagtcatcg tggagtcgcc tgctaaggcc
    10801 aagacgattg aaaaatatct aggcagaaac tacaaggttt tagccagtgt cgggcatatc
    10861 cgtgatttga agaaatccag tatgtccgtc gatattgaaa ataattatga accgcaatat
    10921 atcaatatcc gaggaaaagg ccctcttatc aatgacttga aaaaagaagc taaaaaagct
    10981 aataaagttt ttctcgcgag tgacccggac cgtgaaggag aagcgatttc ttggcatttg
    11041 gcccatattc tcaacttgga tgaaaatgat gccaaccgtg tggtcttcaa tgaaatcacc
    11101 aaggatgcag tcaaaaatgc ttttaaagaa cctcgtaaga tcgatatgga cttggtcgat
    11161 gcccaacaag ctcgtcggat cttggatcgc ttggtagggt attcgatttc gcctattttg
    11221 tggaagaagg tcaagaaggg cttgtcagca ggtcgcgttc agtccattgc ccttaaactc
    11281 atcattgacc gtgaaaatga aatcaatgcc ttccagccag aagaatactg gacagttgat
    11341 gctgtcttta aaaagggaac caaacaattt catgcttcct tctatggagt agatggtaaa
    11401 aagatgaaac tgaccagcaa tgacgaagtc aaggaagtct tgtctcgtct gactagtaaa
    11461 gacttttcag tagatcaggt ggataagaaa gagcgcaagc gcaatgctcc tttaccctat
    11521 accacttcat ctatgcagat ggatgctgcc aataaaatca atttccgtac tcgaaaaacc
    11581 atgatggttg cccaacagct ctatgaagga attaatattg gttctggtgt tcaaggtttg
    11641 attacctata tgcgtaccga ttcgactcgt atcagtcctg tagcgcaaaa tgaggcggca
    11701 agcttcatta cggatcgttt tggtagcaag tattctaagc acggtagcaa ggtcaaaaac
    11761 gcatcaggtg ctcaggatgc ccatgaggct attcgtccgt caagtgtctt taatacacca
    11821 gaaagcatcg ctaagtatct ggacaaggat cagctcaagc tatataccct tatctggaat
    11881 cgttttgtgg ctagccagat gacagcggcc gtttttgata ccatggctgt taaattgtct
    11941 caaaatggag ttcaatttgc tgccaatggt agtcaggtta agtttgatgg ttatcttgcc
    12001 atttataatg attctgacaa gaataagatg ttaccggaca tggttgttgg agatgtggtc
    12061 aaacaggtca atagcaaacc agagcaacat ttcacccaac cgcctgcccg ttattctgaa
    12121 gcaacactga ttaaaacctt agaggaaaat ggggttggac gtccatcaac ctatgcgcca
    12181 accattgaaa ccattcagaa acgttattat gttcgcctgg cagccaaacg ttttgaaccg
    12241 acagagttgg gagaaattgt caataagctc atcgttgaat atttcccaga tatcgtaaac
    12301 gtgaccttca cagctgaaat ggaaggtaaa ctggatgatg tcgaagttgg aaaagagcag
    12361 tggcgacggg tcattgatgc tttttacaaa ccattctcta aagaagttgc caaggctgaa
    12421 gaagaaatgg aaaaaatcca gattaaggat gaaccagctg gatttgactg tgaagtgtgt
    12481 ggcagtccaa tggtcattaa acttggtcgt tttggtaaat tctacgcttg tagcaatttc
    12541 ccagattgcc gtcataccca agcaatagtg aaagagattg gtgttgagtg tccaagctgt
    12601 catcagggac aaattattga gcgaaaaacc aagcgtaatc gcctattcta tggttgcaat
    12661 cgctatccag aatgtgaatt tacctcttgg gacaagcctg ttggtcgtga ctgtccaaaa
    12721 tgtggcaact tcctcatgga gaaaaaagtc cgtggtggtg gcaagcaggt tgtttgtagc
    12781 aaaggcgact acgaggaaga aaagattaaa taagaggagg ggcccgaaag taaatttcag
    12841 gctccttttg gttagaactt gacaaaatac atcattttat gcgaaactag aagaagatta
    12901 ttttatactc aatgagaatc aaagagcaaa ttagaaagct agtcgcaggt tgctcaaagc
    12961 actactttga ggttgaagat ggaagctgac ctggtttgaa gagattttcg aagagtatta
    13021 actaggagaa gttatgcgtc ttatctatct gataattggt tttttatcac tggccttggc
    13081 tattgttggg gttgttttac ccttgttgcc gacaacgcct ttccttttgt tgtctattgc
    13141 ttgtttctcc agaagttcca agcgtttcga agactggctt tatcatacca agctctatca
    13201 aacatatgta gctgattttc gcgagactaa gtctattacg cgtgaacgaa agaaaaaaat
    13261 catcgtctct atctacgtct tgatgggaat ttctatttat tttgcacctc ttttaccagt
    13321 caaaatcggt ctgggtgctt tgaccatctt tattacttat tatctcttca aggtcattcc
    13381 agacaaagaa tagttaaaac agtagttatt tgccttgata aaattgaaag catattcata
    13441 acaatatgat ataataaatt tgaagtaata ttcaaggaga atcaaatgat ttacgaattt
    13501 tgtgctgaaa atgtgacttt acttgaaaaa gcgatgcagg ctggagctcg tcggattgaa
    13561 ctctgtgata atctagcagt tggtgggaca acacccagct atggagtgac taaggcagcg
    13621 gttgaactgg cagctaacta cgatacaacc atcatgacca tgattcggcc acgtggtggt
    13681 gactttgtct atagtgactt agaaattgct atcatgctag aagacattcg tttgactgct
    13741 caggctggaa gtcaaggggt tgtatttgga gctttaactg ctgataaaaa gttggataag
    13801 cctaatctgg aaaagttaat tgctgcatca aaaggaatgg aaattgtctt tcacatggcc
    13861 tttgatgaac taagtgatga agatcaaccg gaagctattg actggctcag tcaagccggt
    13921 gtcactcgta tcctaactcg tgctggtgtg tctggcgact ccttagaaaa acgttttgtt
    13981 cactatcaca gaattttgga gtacgctaaa ggtaaaattg aaattctacc aggtgggggg
    14041 attgaccttg aaaaccgtca aacctttatc gaccaggtgg gggtaacaca attgcatggt
    14101 actaaggttg ttttttaaaa aatagaaagg aactgctagc tttgggtagc agttttcact
    14161 tatgtttgaa atttttaaat cctatcaatt taatcaagaa aaggctcatg attatggttt
    14221 tatagaaaat agcgaagtct ggacatatag ttgccagatt ttgcaaggtg actttgtcat
    14281 gactgtgtcc atcactgctg ataatgtgaa ctttcaagtc tttgaccaag aaactggtga
    14341 cctctatcct cacgttcata tggaaagcat gaggggaagt tttgtcggaa atgtccgtga
    14401 ggcttgtctg gagattcttt accagattcg gaaggcttgt tttgatgtgc aagattttat
    14461 ctgtcatcag actaagcgta tcatgactca agttcaggaa aagtatggaa accagttgga
    14521 gtatctgtgg gaaaaatcgc ctgatacagc tgtattgcgc catgaaggca atcaaaagtg
    14581 gtatgccgtc ttgatgaaaa tctcttggaa taagctggaa aagggaagag aaggacaagt
    14641 ggaagcagtc aacctcaagc atgaccaagt agctaattta ctttcacaaa aggggattta
    14701 tccagccttt catatgagca agcgctactg gattagtgtg tcccttgatg atactttatc
    14761 agatgaagaa gtactggaat tgatagaaaa aagttggaac ttaacctcta aaaaatgaaa
    14821 tattttaata attttcatga actttcaatt agctaaatat tctttactga agaaattttc
    14881 agaaaatata gaatttacca caccagggga atatggtgcc atcttcaaat acctgattga
    14941 gaatgtcaag acggatcgtc agatcatctg ttcgcctcac tgtcatgatg acctcggaat
    15001 ggcagtggca aatagccttg ctgctgtcaa gaatggtgca ggacgtgttg aagggactat
    15061 caatggtatt agggagcgag ctggaaatgc tgctttggaa gaaattgcag tggctctcaa
    15121 tattcgccaa gattactacc aagtagaaac cagtattgtc ctaaatgaga ccatcaatac
    15181 gtcagaaatg gtttctcgtt tctctggtat tcaccaagat ggagtcctta aaaatcctct
    15241 cacttatgag atcatcacac ctgaattggt tggtgttaag attccgcttg gaaaattatc
    15301 tggtcgccat gcttttgttg agaaactgag agaattggcc ctagatttta cagaagagga
    15361 tatcaaacca ctctttgcta agttcaaggc actggccgat aagaagcaag aaatcacaga
    15421 tgcagatact cgagctttgg tagctggaac catggttgaa aatccagaag gcttccactt
    15481 tgatgattta caacttcaaa ctcatgcaga taatgacatt gaagcgctcg ttagcctagc
    15541 caatatggat ggtgagaaag tcgaatttaa tgcgacaggg caaggttccg ttgaagcaat
    15601 ctttaacgct atcgataagt tctttaacca atctgttcgt ttggtgtcct acactatcga
    15661 tgcagtaaca gatggaatcg atgcccagga tcgggttttg gtcactgttg aaaacagaga
    15721 tatagaaacc atctttaatg cagcagggct tgattttgat gtgttgaagg cttctgctat
    15781 tgcctatata aacgctaata cctttgttca aaaagagaat gcaggtgaga tgggatgcag
    15841 tgtttcttac catgatatgc ctagtgtgta aaggagaagg ctatggcaaa gaaaatagta
    15901 gctctagtag gagacggaat tggcccagaa atcatggagg ctggtttaga agttctggag
    15961 actctagctg aaaaaacagg ttttgactat gagattgaca gacgaccgtt cggaggtgca
    16021 gatattgatg cagcaggacc tcccttacct gatgaaaccc ttaaggcaag tagggaagca
    16081 gatgctatcc tactagtagc tatcggtagt cctcagtatg atggagtagc ggttcgccct
    16141 gaacaaggcc tgatggctct ccgtaaggaa ctcaatcttt acgctaatat tcgtcctgta
    16201 aaaatctttg acagtctcaa gtatttgtca ccactcaaac cggaacgaat ttctggtgta
    16261 gactttgtcg tggtgcgtga attgacaggc gagatttact ttggagatca tattcttgaa
    16321 gagcgcaaag cgcgtgatat caacgactat agctatgagg aagtggagcg gattattcgc
    16381 aaagcctttg aaattgcaag aaatcgcaga aaaatcgtta ctagtatcga taagcaaaat
    16441 gttctagcga cctcaaaact ctggcggaaa gtagctgagg aagtcgcaca ggatttccca
    16501 gatgtaacct tggaacacca gctggtagac tcagctgcta tgcttatgat taccaatcct
    16561 gctaagtttg atgttattgt aacggagaat ctttttggag atattttatc tgatgaatca
    16621 agcgtcttat ctggtacact tggggttatg ccatcagcca gtcattctga aaatggacca
    16681 agtctctatg aacctattca cggttcagca cctgatattg caggtcaagg aattgccaat
    16741 cctatttcca tgattttatc agttgtcatg atgttgagag atagtttcgg acgttatgag
    16801 gatacagagc gtatcaaacg tgctgttgag acaagtctgg cggcaggaat tttaacgaga
    16861 gatataggag gtcaggcttc aacaaaggaa atgatggaag ctattattgc aaggttatga
    16921 agttagacga aaaaattact ctagtccttt tgatttggaa tgtcatcatt ttcttgattt
    16981 atggcattga caaattcaag gcaagaagaa gagcttggcg catccaagag aaaatcttac
    17041 tcattttagc ccttacttgt ggtggttttg gggcctggtt agctggaatc acttttcacc
    17101 acaagactcg aaaatggtac tttaaaacag tttggtttct tgggatggtg accacactag
    17161 tagccttata ttttatttgg aggtaatgga tggcagggtc ttcgagggaa cacgctgctt
    17221 gggctctagc gaactatggt tttaaggtcg tgattgcagg aactttcggt gacattcatt
    17281 acaataatga actcaataat ggcctgttgc caatcgttca gcctagagag gttagagaga
    17341 aattagccca gctaaaacca accgaccatg taactgtgga cttggaacaa caaaaaatca
    17401 tctcaccagt tgaagaattc accttcgaga tagatagcga gtggaaacat aaactcctaa
    17461 atagtttgga tgatatcggt attaccttgc agtatgaaga gttgattgct gcttatgaaa
    17521 aacaacgacc agcctactgg caggattaga aaaaatagaa aaggaaatat agtaaactga
    17581 aataagatgt aaacaaatga attggagctt aacatccatt tccagcaatt ttttagaaac
    17641 tacagtggac tattctggat tcaacacatt ataaaattat gacaaaacac attcacaaga
    17701 aggctacgac attttaaaag gtgagggcgg atgtatcgct tgccctacta aagttggtta
    17761 cattatcatg accagcaaca aggcagaact tgagcgtaag ttcgcagcca aagaacgtaa
    17821 gcgtaacaaa ccaggtgttg ttctctgcgg tagcatggat gaacttcgcg ctttagcgca
    17881 actcaaccca gaaattgaag cattctacta aaaacattgg gatgaagata ttcttcttgg
    17941 ttgtatcctt ccttggaaac cagaagcctc tgaaaaactc aaagcatacg gggatggccg
    18001 tgaagaactt atgactgatg tacgtggtac tagctgtttt gttatcaagt ttggaaaagc
    18061 aggtgaacaa ttggctgcca agctttggga agaaggtaaa atggtctacg cctcatctgc
    18121 ttcaatgaca aaacgattga aactcgctat gagagcaagg tgtaatggtg tctatggtcg
    18181 ataaggatgg caaactcatc ccagaacaag gaggagcacg ttcaacctca ccagcaccag
    18241 ttgtgatccg taaagggctt gacattgata aaatcatgat gcacctgtca gatactttta
    18301 actcatggga ctaccgtcag gttgagtatt attaggatag agaagaagtc tagtgttaag
    18361 gacattacag ctcataatac taggcttttt tatagaaatt cttttctttt tctataggat
    18421 atggtattct atgtagaaaa tatatgttaa taagtaatgc caatatttaa acatcattag
    18481 taaaaggagt tagattgatg aataaaagaa aagttagttt agaagatttt tataaatggt
    18541 atagtctaaa taaagaagag ttattaaata aggcaactgt tggtgaaaag tttaatgata
    18601 aattaaaaga agagtttctc caggaatggc ctttggatag gattttaaca atgtcaatcg
    18661 atgaatatgt aataggaaag ggacagcaaa ataagtcttt atgctacgct cttgagaagg
    18721 gaaaatacaa aaatctattt cttggaattt ctggtggctc agcttcaaaa tttggtattt
    18781 attggaataa aaaaacaaac aaatataaag atcaagctaa taatgagatt tcagagttgg
    18841 atcagcgatt ttcaaaatta aaatcagatt tgtatgaaat tatcaaagaa ggtattcgtt
    18901 ttaactttga aaatcctatt tttgatatga aaagatcaac aaatgaattt attggtcgtt
    18961 ctgctatggt gacaaaatta ctttgtatct attctgaggg agatcctttc tttggtgtaa
    19021 atattaatag tcagaaagaa ttttggaacc actttgtttc tcagacaaat caaggtggac
    19081 cttatctgca aaatcataaa ataattgaac tggtgtccaa aacttatcct gagttggagc
    19141 catcgaaatt aggaactatg ctttttgagt attctaagct ttttatggaa aataaaggaa
    19201 gacaatagta caatggattc atcaaacaat tttcgtcatc aattaactca atctctatta
    19261 aagtctccaa accttatcct tcgtggtgct cctggcacag gaaagactta tcttgctaaa
    19321 gaaattgcca aagaattaac ggatggcaac gaagatcaaa tcggatttgt acaatttcac
    19381 ccatcatatg attatacgga ttttgtagaa ggtttaagac cagtatcaaa tggggatgga
    19441 gctattgagt ttaggctaca ggacggtatt tttaaagatt tttgtcagaa agcaaaagaa
    19501 acccaattga ttggaggaca agataatttt gatgaggctt gggattctta cttagaatat
    19561 ataaatgttg ctgaagaaaa agaatatata acaaaaacat cttacttatc tgttaatagt
    19621 agacaaaatt tgtcagtaaa ttatgatagt ggtgttccag gatggtcact acctagcaaa
    19681 tatgtttacg agttgtataa agataaaaat tataataagc aagaatacta caaaagtggt
    19741 ggaaaaactg tcctagaaac attgagaaag agatttggtt tgaaagacta tgtttcccca
    19801 acagaaattg atactgataa gaaattcgtc ttcatcatcg atgaaatcaa tcgtggggag
    19861 atttctaaga tttttggcga actctttttc tctatcgacc ctggctatcg tggtgaaaaa
    19921 ggaatcaacc gtgaggactt attccagctg atttcctatt cttatatttt aaaagctgag
    19981 aaggctggac tgatttttcc tagtatggag cagtcagtaa atagtgaaat aggaaaagta
    20041 gctggctatg gagctcaatt gaagaagtgg tctattcgaa tccctcagaa tgcctcattc
    20101 tatagtacat tttgtaaaat gatggaaaat tcagaagaaa attttaaagc gattattgat
    20161 gaagaagtgg ggagaaaata atttcccctc tttcttttat ttttaacaca agtaaaaccc
    20221 gaacgatata ttttaattca taaaataatt tgacaaaaac tgtactttga tttataataa
    20281 attaaggaaa cgtcggaaaa ggcgtagtga tgcaagagca taggtaggtc attacaaaag
    20341 aaacgagaca tcgatatgtt aaatgaattt ccaatttttg attacgaaga tattcaattg
    20401 attccaaata aatgtgtcat taaaagccgt gcagaagcgg atacaagtgt cactttagga
    20461 aatcacacct ttaaactacc tgttgtgcca gcgaatatgc agacgatttt ggatgaaaat
    20521 gtagcagagc aactggctaa aggtggttac ttctacatta tgcaccgttt tgatgaggta
    20581 ggacgcattt cttttattaa acgaatgcac gatcaagggc tcattgcttc tatctctgtc
    20641 ggtgttaagg attatgagta tgattttgtt agccagctca aggctgatgc tcctgagtat
    20701 atcactattg atattgctca tggtcatgcg gatagcgtga tttctatgat tcaacacatc
    20761 aagaaagaat tgccagatac ctttgtcatt gctggaaatg tgggaacacc agaagctgtg
    20821 cgtgaattgg aaaatgctgg tgcagatgct actaaggtcg gaatcggtcc tggtaaggtt
    20881 tgtatcacca aggttaagac tggttttggt acaggtggtt ggcagttggc tgctctacgc
    20941 tggtgtgcca aggctgcgcg taaaccgatt atcgctgatg gaggaattcg tactcacggt
    21001 gatattgcca agtctatccg cttcggtgct agcatgatca tgattggttc cctctttgca
    21061 ggacatattg aaagtccagg gaaaacgatt gaagtcgatg gtgaacagtt caaagaatat
    21121 tatggttcag cctcacaata tcaaaaaggt gcttacaaaa atgtggaagg caaacgtatc
    21181 ttgcttcctg ctaaaggtca tctgcaagac actttaactg agatggaaca agaccttcaa
    21241 agtgctattt cttatgcagg tggacgtcag gttgctgacc ttaaacacgt tgattatgtg
    21301 atcgtgaaaa actccatctg gaatggggat gcttcccact aagacggggt ctttgaccaa
    21361 aaaacttgtt attagagcaa atttctgtta taataaaaca agtttccacc cttagtgtaa
    21421 tggatatcac gtaagattcc ggttcttgag atgggggttc gattccctca gggtggatgt
    21481 aaacattcta aaaagccttt aatagggctt ttttctttac actgctctaa atttactgct
    21541 aaaactgaga atctctgtct aaagaatttc caaatgcttt ccagtcccgt tttagagtga
    21601 ctcagggggc tttttttgat ataataaaaa ggactgttat cagttagaaa gaggttggta
    21661 tgaaagaatt acaaactgta ctaaagaacc attttgcaat cgaatttaca gacaaaaagt
    21721 tactggaaac tgcctttact catacgagtt atgccaatga gcaccgcctc ttaaaaattt
    21781 cacataatga acgcttggaa tttttaggag acgctgttct acagttattg atttcagaat
    21841 atctgtataa aaaatatcct aaaaagcctg aaggtgacct atcaaaactc cgtgctatga
    21901 ttgtccgtga ggagagttta gctggttttg cgcgtgattg ccagtttgac cagtttatca
    21961 agttgggtaa aggggaagaa aaatctggtg gtcgcaatcg tgacaccatt cttggtgatg
    22021 cctttgaagc ctttcttggt gcccttcttt tggataagga tgtggccaag gtcaaggaat
    22081 ttatctatca agtcatgatt cctaaggttg aagcaggcga atttgagatg attacagact
    22141 ataaaaccca tctccaagag ttacttcagg tcaatggtga tgtggctatt cgttatcagg
    22201 tgatttctga aacagggcct gctcacgata aggtctttga tgtagaagtt cttgttgaag
    22261 gtaagagtat cggtcaaggc caaggtcgtt ctaagaaatt agcagagcag gaagctgcca
    22321 aaaatgccgt tgagaagggg ctggattcat gtatttaaag gaaatcgaaa ttcaggggtt
    22381 caagtctttt gctgataaga ccaaggtcgt ttttgaccaa ggtgtgacgg cagttgttgg
    22441 acccaatgga tctggaaagt ccaatattac agaaagtctg cgttgggctt tgggggagtc
    22501 tagtgtcaag agtctccgtg ggggcaagat gccggatgtc atctttgctg gaacagaaag
    22561 tcgcaaaccg ctcaattatg cttctgtagt tgtgactctg gataatcatg acggatttat
    22621 caaggatgca ggtcaagaaa tcagggtgga acgccatatc tatcgtagtg gagatagcga
    22681 atacaagatt gacggcaaga aagtccgtct gcgtgatatt catgacctct tcttggatac
    22741 tggattggga cgagattcct tctctattat ttcccaaggg aaggttgagg agatttttaa
    22801 ttccaagcct gaggaacgac gagctatttt tgaagaagct gctggagttt taaaatacaa
    22861 gactcgcaga aaagaaaccg agagtaaact gcagcaaact caggataatc tggaccgctt
    22921 agaggacatt atctacgagt tggataatca aatcaagcct cttgagaagc aagctgagaa
    22981 tgcccgtaag tttttagact tggaaggaca acgtaaggct atttatttag acgttctggt
    23041 tgctcaaatc aaggaaaata aggctgaact agagtcgaca gaagaagagt tggctcaggt
    23101 tcaagaactc ttgatgagtt attaccaaaa gcgtgaaaaa ttagaagaag aaaatcaaac
    23161 tcttaaaaag caacgccaag atttacaggc tgaaatggcc aaagaccaag gcagtttgat
    23221 ggatttgacc agtctgatta gtgacttaga gagaaaatta gccctatcga aactggaatc
    23281 tgagcaagta gccctcaatc aacaggaagc acaagcccgt ttggctactt tggaggataa
    23341 gagaagttca ctcagccaag aaaaatctga taaagaaagc tctttagccc tcttggaaga
    23401 aaatctagtc caaaataatc aaaaactcaa tcgattagag gctgaattgc tagctttttc
    23461 agatgatcct gatcagatga ttgagctctt acgtgaacgc tttgtggccc ttttacaaga
    23521 agaagcagat gtctcaaacc aactgacccg tattgagaat gagttggaaa atagtcatca
    23581 gctttctcaa aaacaagcag atcaactaga aaagctgaaa gaacagttgg ctacagctaa
    23641 agagaaggct ggtcagcaaa aggctgagct tgaaactgcc aaggagcagg ttcagaaatt
    23701 attggccgac tatcaagtct gtgctaagga gcaagaggag cagaaaactt cctatcaagc
    23761 tcaacaaagt caactctttg accgtctgga tagtctcaaa aacaagcagg ctagagcgca
    23821 aagtttggaa aatatcctga gaaatcatag taacttttat gcaggtgtta agagtgttct
    23881 ccaagagaaa gatcgtctag gtgggattat tggagcagtt agtgagcacc tgacctttga
    23941 tatgcattat caaactgccc tagaaattgc gcttggagcg agtagtcagc acattattgt
    24001 agaagatgag aatgcggcaa ccaaagccat tgatttactc aaacgaaaca gatctggtcg
    24061 tgcaaccttc cttcctttga ccactatcaa ggctcgtacg atttctagtc agaaccaaga
    24121 tactattgct gcaagtccag gatttctggg catggcagat gagctggtta ctttcgatac
    24181 tagactggaa gccattttca agaatttgct agctacaacg gccatttttg ataccgtgga
    24241 gcatgcgcgt gcagcagcgc gccaggttcg ttatcaggtt cgtatggtga cattggacgg
    24301 aacagagctg cgcacaggtg gttcttatgc aggtggagct aatcgccaga ataacagtat
    24361 tttcatcaag ccagaactgg agcaattaca aaaagaaatt gctgaagaag aagcaagttt
    24421 gcgttcagat gaagtgagtt tgaagaactt gcaagatgag ctagctagat tgacagaaag
    24481 attagaagcc atcaaatctc agggagagca ggctcgtatt caggagcaag gcttgtccct
    24541 cgcctatcag cagaccagtc agcaagttga agagctagaa actctttgga aactccaaga
    24601 agaggaatta aatcgtcttt ctgagggaga atggcaagcg gataaggaaa aatgccaaga
    24661 gcgccttact acaatcacta gtgaaaagaa taatctggaa gctgagattg aagagattaa
    24721 gtctaataaa aacgccatcc aagaacgtta tcaaaacttg caggaagagc tagctcaagc
    24781 tcgtctactt aagacagaac tgcaagggca aaaacgttat gaagttgctg atattgaacg
    24841 cttaggtaag gaactagata atcttgatat tgagcaagaa gaaattcagc gccttcttca
    24901 agaaaaggtt gacaatcttg agaaagttga tacggatttg ctcagtcaac aggctgatga
    24961 agccaaaact caaaaaacaa atctccaaca aggtttgatt cgcaagcagt ttgagttgga
    25021 tgatattgag ggtcaactgg atgatattgc tagtcatttg gatcaggctc gccagcagaa
    25081 tgaagagtgg attcgcaagc aaactcgtgc tgaagccaag aaagaaaagg ttagcgagcg
    25141 cttgcgccat ctacaaaatc aattaacaga ccagtaccag attagctata ctgaagcact
    25201 agaaaaagcg catgagttgg aaaatctcaa tctggcagag caagaggtgc aggatttaga
    25261 gaaggctatt cgctcactgg gacctgtcaa tttagacgct attgaacagt acgaagaagt
    25321 tcacaaccgt ctggatttcc taaatagcca acgtgatgat attttgtctg cgaaaaacct
    25381 gcttcttgaa accatcacag agatgaatga tgaggtcaag gaacgcttta aatcaacctt
    25441 tgaggccatt cgtgagtcct ttaaagtgac cttcaagcag atgtttggtg gaggtcaggc
    25501 agacttgata ttgactgagg gcgacctttt aacagctggt gtggagattt ctgttcaacc
    25561 tccaggtaag aaaatccagt cgcttaacct catgagtggt ggtgaaaaag ctctatcggc
    25621 tcttgccttg cttttctcca ttattcgtgt caagaccatt ccttttgtca tcttggatga
    25681 ggtagaggct gcgctagacg aagccaatgt taaacgtttc ggggattatc tcaaccgctt
    25741 tgataaggac agtcaattta ttgtcgtaac ccaccgtaag gggaccatgg cagcggccga
    25801 ttctatctat ggagtgacca tgcaagaatc gggtgtttca aagattgttt cagttaagtt
    25861 aaaagattta gaaagtattg aaggatgaca attaaactag tagcaacgga tatggacgga
    25921 accttcctag atgggaatgg acgctttgat atggatcgtc tcaagtctct cttggcttcc
    25981 tacaaggaaa aagggattta ctttgcggtg gcttcgggtc ggggatttct gtctctggaa
    26041 aaattatttg ctggtgttcg tgatgacatt attttcatcg cggaaaatgg cagtttggta
    26101 gagtatcaag gtcaggactt gtatgaagcg actatgtctc gtgactttta tctggcaact
    26161 tttgaaaagc tgaaaacgtc accttatgta gatatcaata aactgctctt gacgggtaag
    26221 aagggttcat atgttctaga tacggttgat gagacctatt tgaaagtgag tcagcattat
    26281 aatgaaaata tccaaaaagt agcgagtttg gaagatatca cagatgacat tttcaaattt
    26341 acaaccaact tcacagaaga aacgctggaa gctggtgaag cttgggtcaa tgataatgtc
    26401 cctggtgtca aggctatgac aactggcttt gaatctattg atattgttct ggactatgtc
    26461 gataagggtg tagctattgt tgaattagct aaaaaacttg gtatcacaat ggatcaggtc
    26521 atggcttttg gagacaatct taatgactta catatgatgc aggttgtggg acatcctgta
    26581 gctcctgaaa atgcacgacc tgaaatttta gaattagcaa agactgtgat tggtcaccat
    26641 aaggaacggt cggttatagc ttatatggag ggcttataat ggcagatata aaattgattg
    26701 cattggactt ggacgggacc ttgctgacta ctgataaaag gctgacggat cgtaccaagg
    26761 aaaccttgaa agctgcgcgt gatcgtggta tcaaggtcgt attgacaact ggtcgtccct
    26821 taaaagccat ggatttcttt ctccatgagt tagggactga cggtcaggaa gatgagtata
    26881 ccattacttt taatggtgga ttagttcaga aaaatacagg agaaatcctt gataaaacag
    26941 tcttttcata tgatgatgtg gcacgtttgt atgaagaaac agagaaatta tcactgcctc
    27001 tcgatgccat ctcagaagga acagtttatc aaatccaatc ggaccaagaa agtctttatg
    27061 ccaaattcaa tccagctttg acctttgttc cagtggactt tgaagactta tctagtcaaa
    27121 tgacctacaa caaatgcgtg actgcctttg ctcaagaacc cttggatgca gccattcaga
    27181 agatttctcc agaattgttt gaccaatatg aaatctttaa atcacgtgaa atgttgctag
    27241 aatggtcacc aaagaatgtt cataaagcaa caggtttggc aaaactaatc agccatcttg
    27301 gaatcgacca aagtcaagtg atggcttgtg gtgacgaggc caatgacctc tctatgattg
    27361 aatgggcagg tcttggtgtt gctatgcaaa acgctgttcc tgaagtaaag gcagccgcaa
    27421 atgtagtgac gccgatgacc aacgatgagg aagctgtcgc ctgggctatc gaagaatatg
    27481 tgctaaagga gaactaagat atgggattgt ttgaccgtct attcggaaaa aaagaagaac
    27541 ctaaaatcga agaagttgta aaagaagctc tggaaaatct tgatttgtct gaagatgttg
    27601 atcctacctt cacagaagtt gaggaagttt ctcaggaaga agcagaggtt gaaattgttg
    27661 aacaagctgt gttccaagaa gaggaaatcc aagacacagt tgaagaaagt ctggatttag
    27721 agccagttat agaagtttct caaaaagaag tcaaagaatt tccacactca gaagaaggga
    27781 atactgagtt tctagagact gtagaagaaa ataattctga aattcttgaa gcagaaaggc
    27841 ctcaaacaga agaaactgtt caggaaaaat atgaccgcag tcttaagaaa actcgcacag
    27901 gtttcggtgc ccgcttgaat gccttctttg ctaacttccg ctctgttgac gaagaatttt
    27961 tcgaggaact ggaagaactg ttgattatga gtgatgttgg tgtccaagtc gcttctaact
    28021 taacggagga actacgttac gaagccaagc ttgaaaatgc caagaaacct gatgcacttc
    28081 gtcgtgtcat cattgagaaa ttggttgagc tttatgaaaa ggatggtagc tacgatgaaa
    28141 gcatccactt ccaagataac ttgacagtta tgctctttgt tggtgtgaat ggtgttggga
    28201 aaacaacttc tatcggaaaa ctagctcacc gctacaaacg agctggtaag aaggtcatgc
    28261 tggttgcagc agataccttc cgtgcgggtg cagtagctca gctagctgaa tggggccgac
    28321 gagtagatgt tccagtagta actggacctg aaaaagctga tccagccagc gtggtctttg
    28381 atggtatgga acgtgccgtg gctgaaggta tcgatattct catgattgat actgctggtc
    28441 gtctgcaaaa taaggataac cttatggctg agttggaaaa gattggtcgt attatcaaac
    28501 gtgttgtgcc agaagcacca catgaaacct tcttggcact tgatgcatca acaggtcaaa
    28561 atgccctagt acaggccaaa gaattttcga aaatcacacc tttaacggga attgttttga
    28621 ctaagattga tggaactgct cgaggaggtg tggttctagc cattcgtgaa gaactcaata
    28681 ttcctgtaaa attgattggt tttggtgaaa aaatcgatga tattggagag tttaactcag
    28741 aaaacttcat gaaaggtctc ttggaaggtt taatctaatc agaagcaaaa atcctgcaag
    28801 gcataaactt gcaggaaatt tttttattct aagcgaccat cttgacgata ggcgatatct
    28861 ggttgccaag tccatttggc accgaatttt tcaagtaggt caaagctggc ttgaggtccc
    28921 atgcttccag ctttatagtc atgaagtggg gcaccatttt catcccagag ctcttcaata
    28981 cggtcaatca acttccatga tgcaccaact tcatcccagt ggctaaagtt agttgagttg
    29041 ttatttagga catcataaat caatttctcg tatggttctg gagaagcacc agttgcagtc
    29101 gcatctgtac ggtaatcaag tgagttagga gccaagttaa attcttctcc tacttgcttc
    29161 ccatttaggc taagagagaa gccttctgtt ggttgaatat agatggtcaa aatatttgga
    29221 gcaagtggtt ctccaaagat agaatccatt tgtttaaaga cgatgttgac atgagttcct
    29281 ttttcagtca gtcgtttacc tgtacggaaa aagaaaggaa cactacggaa tcgatcgctg
    29341 tctacaaaga aggcaccaga tgtaaaggtt tcagttgttg attctggatt cacatttggc
    29401 tcgctacgat aagagatgta tttcatgcca tcaatcttac cagagcggta ttgcccacgg
    29461 ataaagtgtt ctttgagttc ttcatcagtt ggatgataga ggtttttaaa gaccttaatc
    29521 ttttcagcac gaatctcgtc ttttgtgaag cttgctggtt tgtccatggc gaggagcgaa
    29581 agaagttgta gagtgtggtt ttggaccatg tcacggaggg caccggattg gtcatagtag
    29641 ccaccacgtt cttctacacc caagcgctcc gcaaaggtaa tttgaacatt gtcgataaaa
    29701 tccttgttcc aaacgttttc aaaaatcaag tttgcaaagc gaactgcaaa gatgctttgg
    29761 atcatttcct taccaagata atggtcgata cggaaaattt gttcttcgtc aaatgttgct
    29821 aggagttcgt cattcaactt gcttgcagtt gcgtaatctg taccaaatgg tttttcaacg
    29881 atcaagcgct caaaaccttt gccatcgaca atgttttcag atttgaggtg tttggcaatg
    29941 gttccaaaga actgaggtgc catagacaag aagaagagct tattgtgttc agcttggtat
    30001 ttttcattaa gctcagcttg taattgacgc aaagcaatat aatgttccga atcattgaca
    30061 tcatggcttt gatagtagaa gtggctagca aattcttggg cttgctcggt actatctgcc
    30121 aaatcaagga tggactcgac aactacagat tcaaaatatt ccttactcca aggtctacgg
    30181 gcagttccaa taacggcaaa gtgcttggaa agattgccgg atttatatag tctaaaaagg
    30241 gaagggtaga gcttgcgttt agccaggtct ccactcgcac cgaaaattgt aacaataacc
    30301 ttagatgaca tctagctacc taatttctat ttttattcct agtcttgcta ggtatgagga
    30361 aacctcattt cataaactta attctaacat aattttccga aaaatcaaaa attccaatac
    30421 gaagtagaaa aactgcaagg aaatccttac agtttgagtt tagacgttta agaccttatc
    30481 taagaactct ttcagacgag ggtgttgtgg gttatcaaag atttggtcag gtgttccgtc
    30541 ttcaaggaac tcgccatctg cagtaaagat aacgcggttg gcaacctgac gagcaaatcc
    30601 catctcatgg gttacgataa tcatggtcat accttgctca gccaattcct tcataacgtt
    30661 aagtacgtct ccaaccatct cagggtcaag ggcagaagtt ggttcatcga agagcatgat
    30721 gtctggattc attgctaggc cacgagcgat ggccacacgt tgtttttgac cacctgatag
    30781 gctatctgga ttggcattag ctttatctgc tagtccaacc ttttcaagca actccattcc
    30841 caattcctca gcttcttcct tagtcatcaa cttgtgctca ataggagcaa aggtgatgtt
    30901 gtccaataca gacatatgag ggaagaggtt gaagtgttgg aataccatgc cgatattttc
    30961 acggacgtgg tcaacattgg ttgttttttc agttaaatca tagccgttca cagtgatgtg
    31021 accgctagtg acttcttcta aaagattgag gctacggagg aaagttgact taccagaacc
    31081 tgaaggaccg atgatacaaa caacatctcc ttcatagaac ttagtcgtaa ttccttttag
    31141 gacttcattt tttccatagt gcttgtgtaa atcatttaca tcaattttta attttgccat
    31201 taacgaatcc tcttttctaa gcgtttcgct agtctagtca aaagtgtgat aattacaaga
    31261 tagaagatag caaggattgc atacatcttg aaactttggt agttacgagc aatgataatc
    31321 ttaccagttt ggaagagttc aaccaaaccg atagcagata cgatagttgt atctttaaga
    31381 gcgataacga attggttgac aaagtttggc aacatcaatt tagttgcttg tggcaagata
    31441 atcttacgca tggtttttcc ataagagata cccaagcttc ggctggcttc catttggcca
    31501 actggaacgg cctgaatacc accacgaacg atttcagcga tataagcagc cgcattgagt
    31561 gagagggcaa tggttccagc tacaaagtcg ttaattgggc tttgttggcc tgtgatagac
    31621 tcgatgaagt ttggaattcc ccagaagatg aaggctgcaa gaatcatcaa tggaatacca
    31681 cgaataacgt caacgaaaat ctcagagatg acgcgaagag atttgtatgg gctaacgcta
    31741 aacataccga agataattcc gatgacaatg gcaatagcaa atgagataag agctagagca
    31801 agagtgatac caagaccgct aaggagttgt ttgtagttgt tttgaagcaa gccccagagc
    31861 gttgtttcgt caacagtact tgttgaagca gttgaagatt cgctagctag gtatttgtca
    31921 agaatctttt ggaattcacc gtttgcttta aggtttgcaa gtccgttgtt gaacatttca
    31981 atcagttctg gatttgctcc ttttttaacg gcaaaggctg tttcaccgat tggagttcca
    32041 gagattggag ttttcaattt ttgaccttgg ctgatagaat atttgagaac aggttcatca
    32101 tccataacgg catcaatggc accagtgttt aaactgtcat acattgaaga accatcagca
    32161 aaggttttga ttttgtaacc gtatttgctt tgattttctg ttaggaaggt ttgagaagca
    32221 gttccgtttt taacaccgac tgtctttcct tttagatctt cataagaagc aatattgctt
    32281 gattctttga caccaagaat ggtattagca gtgtagtatg attctgagaa gtcaaaagtt
    32341 gccttacgag catctgtgac agacatccca gcaatgatac catcggcttg accagcttgg
    32401 acagcactga tagcagcatc aaaaccaggg ttggtgattt caatttcaaa accttggtct
    32461 ttagcgattg ccttaatcaa ttccatatca ataccagtgt attggttgct tgaattttgg
    32521 aaaacaaaag gtgcaaaaga agaatcgctg gcaatgatat atttagcctt aacaggaata
    32581 gcttttaatc ctgcgagagt agttgtagtt ggcactgctg aagatgatga agcagtccat
    32641 ttcttgataa ttttatcaag actaccatct tttttcattt cagccaaggc ttggttaaat
    32701 tcagtaacca ggtgctcgta tttacttcct tttttcacac cgaaagcaaa acttcctaca
    32761 gcttcaccat ccatttcaat atggagatct tgaccttggt taatggcata ttcgataaca
    32821 ggtttgtcat ccatcatggc atcgatggca ccagcactca agctgttgtt cattaaatca
    32881 ccagtgtcaa atgttttaat agtaaagccg tatttatctt tgattgtttc aaggaaacgt
    32941 tgagcggcag ttccgttttt aacaccaacg gttttgccag ttaattggtc gtacttgcta
    33001 attttgtgtg actttgtagt agcaatgaca acttttgtat catagtaagt atcagacatg
    33061 gtgaagactt tttcacgttc tttagtcttt gtcatccctg ccatgatagc gtcggcttgc
    33121 ccagcttgaa ccgcattgac tgctgcgtca aatccaggat aggacatctg aatgttccag
    33181 cctttaatct cagcgacttt gttaataatg tcaacatcaa ttcctttata agtttgatct
    33241 gaatctttaa actcaaaagg tgcataggcg gtatcagaaa caatcttaat cgtttctgct
    33301 ttggcaatac ctaatgagaa aattgggaat aaaattagca aaaatgctag aaattttttc
    33361 ttcattttta gtctcctttt ccgaatattc tctcattata tcagaaaaag taaaaaaagg
    33421 caaattgtta tatttttcgt gtcagaaaat ataacattga tttttccttt tctttcttga
    33481 attgctatta aaaagtgcta taataatagt atatatagaa gaaaagagga cggggatatg
    33541 aagaacaaaa gaatatttaa agacttccaa gcttcaaaaa tgaatttaaa catttacaca
    33601 agccccttgt tagcctttgt ttttgtcttc ataggagagt ttgtggctta tactttatat
    33661 ggtattagtt tgttagctct catcggactt gctagaaatt ttggagaggc tggtcaaaat
    33721 cttgcaagct acttgcagac cttgcatcag agcttgacgg ataaaacaag tgactttcgt
    33781 ttaattttag gattactggc ctttggtttt attcttaaca ctgtgttcag atggacaaga
    33841 aaagttgaga aaagacctat tcgaaccttg ggattttata gagagaattt cctcagcaat
    33901 cttctgaaag gatttagtct aggcctggca ctttttcttc tgaccttgtt aggtttagtg
    33961 gtcttaggtc aatatcgttt ggaatccatt cacttgaatc cttattctct tgcctttgtc
    34021 gtctttacta tcccattttg gattttacag gggacagcag aagaagtggt ggcccgtgct
    34081 tggctacttc ctcaattggc ctcaagaacc aatctaaaac tagctattct tatatctagc
    34141 ctgttcttta ccctgcttca tatgggcaat tctggcctca cccctctatc tctagtaaat
    34201 ctctttttat tcggagttgc catggctctt taccttctca aaactgatac agtttggggt
    34261 gttgcaggta ttcatggtgc ttggaatttt gctcagggta atctctttgg gattttagtt
    34321 agtggtcaac cgtcaggaac gtctctgatg acctttttac cacaaggcaa tcaagattgg
    34381 ctatcaggtg gttcttttgg catagaaggt tccattatga caagtctggt attactactg
    34441 ctgattgtct atcttgctaa taaattaaag aaagaaaatg aaaggatgtg acttcggtcc
    34501 gtccttttct tcgtgaaaat actataagta tgctaaaata ggaatagcac atggagagag
    34561 gattcttatg atcaatcaca ttacagataa tcaatttaaa ctagtatcaa aatatcaacc
    34621 atcaggagat caaccccaag ctatcgagca gttggtggat aacattgagg ggggagaaaa
    34681 agctcagatt ctgatggggg cgactggaac agggaagacc tatactatga gtcaggtcat
    34741 ttctaaagtc aataaaccaa ctctggttat tgcccacaat aaaactctgg ctggtcagct
    34801 ctatggggag tttaaggaat ttttccctga aaatgcagtt gagtatttcg tatcctacta
    34861 tgattattac cagccagagg cctatgtccc ttctagcgat acctatattg agaaggatag
    34921 ttctgtcaat gacgagattg acaagcttcg ccactcagct acctcagccc ttttggagcg
    34981 taatgatgtt attgtcgtgg cctcagtctc ttgtatctat ggtttgggtt cgcccaagga
    35041 atacgctgat agtgtcgtta gtctccgtcc tggtctagag atttctcgtg ataaactctt
    35101 gaatgacttg gtcgatattc agtttgaacg taatgatatt gatttccaac gcggaagatt
    35161 tcgtgttcgt ggggatgtgg tagagatttt cccagcttcc cgagatgaac atgcctttcg
    35221 agtagaattt tttggagacg aaattgaccg tattcgtgaa gttgaggctc tgacaggtca
    35281 ggtgttggga gaagtggatc atttagcgat tttcccagcg acacactttg tgaccaatga
    35341 cgaccacatg gaagttgcca ttgcaaagat tcaggccgag ttggaggagc agttagctgt
    35401 ctttgaaaag gaaggtaaac tgcttgaagc ccagcgtttg aaacagcgga cagagtatga
    35461 tatcgaaatg ttgcgtgaga tgggctatac caatggggtt gaaaattatt ctcgccacat
    35521 ggatggacgg agcgaaggag agcctcctta tacgcttctc gacttcttcc cagatgattt
    35581 cttgattatg attgacgaga gtcatatgac catagggcaa atcaagggca tgtacaatgg
    35641 agaccgttcg cgtaaagaaa tgctggttaa ttatggtttc cgtttgccgt ctgctttgga
    35701 caatcgtcct ctccgtcggg aggagtttga gagtcacgtt catcagattg tttacgtttc
    35761 agcgacacct ggtgactatg aaaatgaaca gaccgagaca gtgattgagc aaatcattcg
    35821 tccaacggga ctcttggatc cagaggtgga agtccgtccg actatgggac agattgatga
    35881 cctcttgggt gaaatcaatg cccgcgttga aaaaaatgag cgtaccttta tcacaacttt
    35941 gaccaagaaa atggcagagg acttgaccga ctacttcaag gaaatgggta tcaaggtcaa
    36001 gtacatgcac tcggatatca agaccttgga acggacggag attatccgtg acctgcgctt
    36061 gggtgtcttt gatgtcttgg tcggaattaa cctgctccgt gaaggaattg acgttcctga
    36121 agtgagcctc gtagctattc tcgatgctga caaggaaggt ttccttcgca acgaacgtgg
    36181 actcatccag actattggac gtgctgctcg taacagtgaa ggccatgtca tcatgtatgc
    36241 agacacggtt acccagtcta tgcaacgtgc tatcgatgaa actgcccgcc gtcggaaaat
    36301 tcagatggcc tataatgaag agcatggtat cgtaccacag acaatcaaga aagaaatccg
    36361 tgacctgatt gccgtgacca aggcagttgc taaggaagag gacaaggaag tcgacatcaa
    36421 tagcctcaac aaacaagagc gtaaagaact cgtcaagaaa ctagaaaaac aaatgcaaga
    36481 agccgtcgaa gtgcttgact ttgaactggc ggctcagatc cgtgatatga tgttggaagt
    36541 caaggccttg gattagggga atagtatgat ttatttaaga aagttaaaga gagaagattt
    36601 gatgtcttta tgggaaatgg cttattcaca acttaatcca gtttggaaac agtatgatgc
    36661 tccctattat gatgattatc agtatttttc aaattttaaa gaattcgaac tacaaaaatc
    36721 agaatccatt ttaagcaact caaatcgcct tggtattttt gttgatgata aactagttgg
    36781 gactgtttcg cgttattggg tatgtaaaca aacaagatgg atggaattgg gaattggtat
    36841 ttatgataaa aaattctgga acactggtat tgggaaagtt gctatgttgc agtggataga
    36901 taggacgttt caggattact tggagttgga gcatctgggt ttgacaactt ggtcagggaa
    36961 tcttggtatg atgaaacttg ctgaaaaatt aagaatgaaa aaagaagctc atattccaaa
    37021 agttcgttat tatcaaggta aatattttga tagtattaaa tatggtattt tgagagaaga
    37081 ctgggagaaa ataaatgacg gttattatca aatcaatgga aactcctaaa gagatagaag
    37141 gtaaatccct tgttcactgg caaatgtgga gagaggctta tgatgacctt ttgcctgcag
    37201 aatttcagga gacaatgaca ttagaaagat gtcgactctt tagtcaaaag tatccagaaa
    37261 atacattgat tgcgataggt gatctgaagg tagttggttt tatcagttat ggtaactttc
    37321 gtgatgagac tatgcaagct ggtgaaatta ttgctttata tgttttaaaa gactattatg
    37381 gaaaaggtat cgcacaaaag ttaatgaaag cagctttgac tgctcttaat catttttctg
    37441 aaattttctt atgggtattg aaagataaca agcgcgccat tgctttctat caaaaaatgg
    37501 gttttacttt tgatggacaa gaaaaaatac ttgaacttgg aaagcctata aagga
//
LOCUS       DAVKWZ010000014        36901 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000014 DAVKWZ010000000
VERSION     DAVKWZ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36901
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.014"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            152..1381
                     /gene="arcA"
                     /locus_tag="VW854_000763"
     CDS             152..1381
                     /gene="arcA"
                     /locus_tag="VW854_000763"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEV0262034.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            1439..2455
                     /gene="argF"
                     /locus_tag="VW854_000764"
     CDS             1439..2455
                     /gene="argF"
                     /locus_tag="VW854_000764"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEV0262035.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNIHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            2620..3567
                     /gene="arcC"
                     /locus_tag="VW854_000765"
     CDS             2620..3567
                     /gene="arcC"
                     /locus_tag="VW854_000765"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEV0262036.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVEADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            3778..5289
                     /locus_tag="VW854_000766"
     CDS             3778..5289
                     /locus_tag="VW854_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEV0262037.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            5311..6642
                     /locus_tag="VW854_000767"
     CDS             5311..6642
                     /locus_tag="VW854_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEV0262038.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     PLYEQVCNDLKEAGYDYQSTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVKLLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            complement(7034..7321)
                     /locus_tag="VW854_000768"
                     /pseudo
     CDS             complement(7034..7321)
                     /locus_tag="VW854_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7309..7560)
                     /locus_tag="VW854_000769"
     CDS             complement(7309..7560)
                     /locus_tag="VW854_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV0262039.1"
                     /translation="MKEFQFERKQRFSLRKYAIGACSVLLGTSLFFAGMGAQPVQDTE
                     TSSALILSHYLDEQDLSEKLKSDLQWFELENKLLNLWEH"
     gene            7872..8021
                     /locus_tag="VW854_000770"
     CDS             7872..8021
                     /locus_tag="VW854_000770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262040.1"
                     /translation="MDRTDEVSSKHCFEVADRTNEVSSKHCFEVADRTDEVSSNIRQG
                     DADVV"
     gene            complement(8048..8932)
                     /locus_tag="VW854_000771"
     CDS             complement(8048..8932)
                     /locus_tag="VW854_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV0262041.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(9077..10228)
                     /locus_tag="VW854_000772"
     CDS             complement(9077..10228)
                     /locus_tag="VW854_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEV0262042.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKVLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            complement(10374..12140)
                     /locus_tag="VW854_000773"
     CDS             complement(10374..12140)
                     /locus_tag="VW854_000773"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEV0262043.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAILGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEASDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRS
                     IYDPEEFERALKWVKENVKEGFDHNREDLVLSREEKDRQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            complement(12223..15339)
                     /locus_tag="VW854_000774"
     CDS             complement(12223..15339)
                     /locus_tag="VW854_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEV0262044.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDA
                     SKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTE
                     NWDENTHVYTITVNHNGMVEMSINTEGTGPVSFPTPDKFNDGNLNIAYAKPTTQSSVD
                     YNGDPNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQR
                     LSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVE
                     VFRESDGKQSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVT
                     HTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDK
                     VVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQS
                     STDYNGFSRLAVDGNKNGDYGHHSVTHTKEDSPSWWEIDLAQTEELEKLIIYNRTDAE
                     IQRLSNFDIIIYDSNDYEVFTQHIDSLESNNLSIDLKGLKGKKVRISLRNAGIPLSLA
                     EVEVYTYK"
     gene            complement(15349..17643)
                     /locus_tag="VW854_000775"
     CDS             complement(15349..17643)
                     /locus_tag="VW854_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEV0262045.1"
                     /translation="MKLWYKKAASNWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKDCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPKTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQEREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            complement(17702..18502)
                     /locus_tag="VW854_000776"
     CDS             complement(17702..18502)
                     /locus_tag="VW854_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV0262046.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            complement(18499..19272)
                     /locus_tag="VW854_000777"
     CDS             complement(18499..19272)
                     /locus_tag="VW854_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV0262047.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            complement(19297..19767)
                     /locus_tag="VW854_000778"
     CDS             complement(19297..19767)
                     /locus_tag="VW854_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV0262048.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            complement(19758..20189)
                     /locus_tag="VW854_000779"
     CDS             complement(19758..20189)
                     /locus_tag="VW854_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV0262049.1"
                     /translation="MFNRGILVASHGNFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVNELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISSYDSKINLEEIVQNAQNSLFNVKQQLNVEEEEDLCL"
     gene            complement(20182..20622)
                     /locus_tag="VW854_000780"
     CDS             complement(20182..20622)
                     /locus_tag="VW854_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HEV0262050.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            complement(20634..21272)
                     /locus_tag="VW854_000781"
     CDS             complement(20634..21272)
                     /locus_tag="VW854_000781"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HEV0262051.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMDLMAEKFKTYGQRK"
     gene            complement(21387..22790)
                     /locus_tag="VW854_000782"
     CDS             complement(21387..22790)
                     /locus_tag="VW854_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEV0262052.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLVDNEGKLLLQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPLLTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGLTEATAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            22966..23739
                     /locus_tag="VW854_000783"
     CDS             22966..23739
                     /locus_tag="VW854_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV0262053.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTLPDQKLLDAVELFDGEWVSDIQ"
     gene            complement(24910..26415)
                     /gene="adcA"
                     /locus_tag="VW854_000784"
     CDS             complement(24910..26415)
                     /gene="adcA"
                     /locus_tag="VW854_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEV0262054.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVCLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNIAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(26425..27231)
                     /locus_tag="VW854_000785"
     CDS             complement(26425..27231)
                     /locus_tag="VW854_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV0262055.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            complement(27224..27928)
                     /locus_tag="VW854_000786"
     CDS             complement(27224..27928)
                     /locus_tag="VW854_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV0262056.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYCVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            complement(27928..28368)
                     /gene="adcR"
                     /locus_tag="VW854_000787"
     CDS             complement(27928..28368)
                     /gene="adcR"
                     /locus_tag="VW854_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEV0262057.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            complement(28523..29791)
                     /gene="dltD"
                     /locus_tag="VW854_000788"
     CDS             complement(28523..29791)
                     /gene="dltD"
                     /locus_tag="VW854_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEV0262058.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            complement(29784..30023)
                     /gene="dltC"
                     /locus_tag="VW854_000789"
     CDS             complement(29784..30023)
                     /gene="dltC"
                     /locus_tag="VW854_000789"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEV0262059.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            complement(30037..31281)
                     /gene="dltB"
                     /locus_tag="VW854_000790"
     CDS             complement(30037..31281)
                     /gene="dltB"
                     /locus_tag="VW854_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEV0262060.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMRGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQFLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            complement(31278..32828)
                     /gene="dltA"
                     /locus_tag="VW854_000791"
     CDS             complement(31278..32828)
                     /gene="dltA"
                     /locus_tag="VW854_000791"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEV0262061.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPMVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMKNPEKTAEAFFEFEGLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            complement(32849..32980)
                     /locus_tag="VW854_000792"
     CDS             complement(32849..32980)
                     /locus_tag="VW854_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEV0262062.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            complement(33294..34472)
                     /locus_tag="VW854_000793"
     CDS             complement(33294..34472)
                     /locus_tag="VW854_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV0262063.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTFIILVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
     gene            complement(34928..36204)
                     /gene="ltrA"
                     /locus_tag="VW854_000794"
                     /pseudo
     CDS             complement(34928..36204)
                     /gene="ltrA"
                     /locus_tag="VW854_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT        11235 a         7803 c         6403 g        11460 t
ORIGIN      
        1 gtataataac ataaaattgt gctaaacatc ataatttata ttattttccg agaaattata
       61 gccattgaca ttacctagga attgcgttat aacatacatg taagcggtac ccaaaatggt
      121 gccaagtcaa aatttttaag gaggaaaata catgtcttct catccaattc aggtcttctc
      181 agaaattggg aaactgaaaa aagttatgtt gcaccgtcca ggcaaggagt tagaaaactt
      241 gttgccggac tatcttgaaa ggcttctttt tgatgatatt cctttcttgg aagatgctca
      301 aaaagaacat gatgcatttg cccaagctct tcgcgatgaa ggaattgagg ttctctacct
      361 agaacaactc gctgctgaat cattgacctc tccagaaatc cgcgatcaat ttatcgagga
      421 atacttagac gaagccaaca tccgtgatcg tcaaaccaag gttgctattc gtgaattgct
      481 tcacggcatc aaggacaacc aagaattggt tgaaaaaaca atggctggga ttcaaaaagt
      541 tgaattgcca gaaattcctg acgaagctaa agatctaact gacttagttg aatcagatta
      601 tccatttgca attgacccga tgccaaacct ctatttcact cgcgacccat ttgcaacaat
      661 tggaaacgcc gtatcgctta accacatgtt tgcagacact cgtaaccgtg aaacactcta
      721 cggtaagtat atcttcaaat accacccaat ctatggcgga aaagtggatt tggtctacaa
      781 ccgtgaagaa gatacgcgta tcgaaggtgg agacgagtta gttctttcta aagacgtcct
      841 tgcagtaggt atctctcaac gtacagacgc agcttctatc gaaaaacttt tggtcaacat
      901 cttcaagaaa aatgttggct tcaagaaagt tttggccttt gaatttgcta acaaccgtaa
      961 attcatgcac ttggatactg tcttcactat ggtagactat gacaagttca ctattcaccc
     1021 agaaatcgaa ggcgaccttc acgtttactc agttacttac gaaaacgaaa aacttaaaat
     1081 cgttgaagag aaaggtgact tagctgaact tcttgctcaa aaccttggtg tagaaaaagt
     1141 tcatttgatt cgttgcggtg gtggcaatat cgtagcagct gcgcgtgaac aatggaacga
     1201 cggttctaac actttgacca tcgcacctgg tgtggtagtt gtttatgacc gcaataccgt
     1261 gaccaataag attttggaag aatacgggct tcgcttgatt aagattcgcg gaagtgaatt
     1321 ggttcggggc cgtggtggac ctcgttgtat gtctatgcca tttgaacgtg aagaagtgta
     1381 atcgctgttc ggtattcgtc aatagaaaat gtaaaaaata gaaagaggaa ataataaaat
     1441 gacaaattca gtattccaag gacgcagctt cttagcagaa aaagacttta cccgtgcaga
     1501 gttagaatac cttattggtc tttcagctca cttgaaagat ttgaaaaaac gcaatattca
     1561 acaccactac cttgctggca agaatatcgc tctcctattt gaaaaaacat ctactcgtac
     1621 tcgtgcagcc tttacaactg cggctatcga ccttggtgct cacccagaat acctcggagc
     1681 aaatgatatt cagttgggta aaaaagaatc tactgaagat actgctaaag tattgggacg
     1741 tatgtttgac gggattgaat tccgcggatt cagccaacgt atggttgaag aattggcaga
     1801 attctcaggc gttccagtat ggaacggtct aactgacgaa tggcacccaa ctcaaatgct
     1861 cgctgactac ttgactgttc aagaaaactt cggtcgcttg gaaggcttga cattggtata
     1921 ctgtggtgat ggacgtaaca acgttgccaa cagcttgctc gtaacaggtg ctatccttgg
     1981 tgtcaatatt cacatcttct caccaaaaga actcttccca gaaaaagaaa tcgttgaatt
     2041 ggcagaagga tttgctaaag aaagtggcgc acatgttctc atcactgaag atgctgatga
     2101 agcagttaaa gatgcagacg ttctttacac agacgtttgg gtatcaatgg gtgaagaaga
     2161 caaattcgca gaacgtgtag ctcttcttaa accttaccaa gtcaatatgg acttagttaa
     2221 aaaagcaggc aatgaaaact tgatcttcct acactgcttg ccagcattcc acgatactca
     2281 cactgtttat ggtaaagacg ttgctgaaaa atttggtgta gaagaaatgg aagtaacaga
     2341 cgaagtcttc cgcagcaagt acgctcgcca cttcgatcaa gcagaaaacc gtatgcacac
     2401 tatcaaagct gttatggctg ctacacttgg taacctttat attcctaaag tataatttta
     2461 gataataaac cgtctaccaa cagctatgag ggctgcgact aatagcttta gtccggtcct
     2521 cttttatata atggtaatct attatttctt ataaaatatg tgaaaaatca ttaaattgaa
     2581 atctaaacgc attctattga gtgtgataaa ggagaattta tggcaaatcg taaaattgta
     2641 gtagctttgg gaggaaatgc gattctttct tctgacccat cagcaaaggc tcaacaagaa
     2701 gctttagttg aaacagctaa gcatcttgta aaattgatta aaaatggaga tgatctgatt
     2761 atcactcacg gtaatggacc tcaagttggg aatctcttgc tccaacattt ggcatcagac
     2821 tctgaaaaga accctgcctt cccactcgac tcacttgtcg ctatgacaga aggtagcatc
     2881 ggtttctggt tgaaaaatgc tttgcaaaat gctctcttgg atgaaggcat cgaaaaaaat
     2941 gttgcctctg ttgtaacgca agttgtcgta gataaaaatg atccagcttt tgttaacttg
     3001 agtaaaccaa tcggtccttt ctattcagaa gaagaagcaa aagcagaagc cgaaaaaagc
     3061 ggagcgactt tcaaggaaga tgctggccgt ggctggcgta aggtcgttgc ctcaccaaaa
     3121 cctgttgaca tcaaagaaat tgaaaccatc cgtactcttt taaataatgg tcaagtcgtc
     3181 gtagctgcag gtggtggcgg tattcccgtc gtcaaagaaa acaatggaca tttgactggt
     3241 gtcgaagcgg ttattgataa agacttcgct tcccaacgtt tggcagaatt ggttgaggca
     3301 gacctcttca tcgttttgac aggtgtagat tatgtatttg ttaactacaa caagccaaac
     3361 caggaaaaat tggaacatgt gaatgttgcc cagctggaag aatatatcaa acaagatcag
     3421 tttgcaccag gtagcatgct tccaaaagta gaagcagcta tcgcttttgt caatggtcgt
     3481 ccagaaggaa aagcagttat tacttccctt gaaaatctag gcgccttgat tgaatctgaa
     3541 agcggaacaa ttattgaaaa aggataagtt gttttactaa taagatgtat tctatttcta
     3601 gtatctttat atcaaattag aaattattct tgaaaacatg tacaatattt caaaaaatat
     3661 tagtttttta gactttaata tggtaaaaca aatataaata gaaagcgttt tcttaaatgt
     3721 ttatttaaga aagtagttgg ttttttacac tttgttagac atcaggagga aaaacaaatg
     3781 agtgaaaaag ctaaaaaagg gtttaagatg ccttcatctt acaccgtatt attgataatc
     3841 attgctatta tggcagtact aacttggttt atccctgcgg gggcctttat agaaggtatt
     3901 tacgagactc agcctcaaaa tccacaaggg atttgggatg tcctcatggc accgattcgg
     3961 gctatgctag gtactcatcc agaggaaggt tcgctcatta aagaaacgag tgcagcgatt
     4021 gatgtagcct tcttcatcct tatggttggt ggtttccttg gcattgtcaa caaaactggt
     4081 gctcttgacg tagggattgc ctctatcgtg aagaagtata agggccgcga aaaaatgtta
     4141 attttggtac tgatgccttt gtttgccctc ggtggtacaa cttatggtat gggggaagaa
     4201 acaatggcct tctatccact ccttgtgcca gttatgatgg ccgttggttt tgatagcctg
     4261 actggtgttg caattatttt gctcggttct caaatcggct gtttagcatc tactctgaat
     4321 ccatttgcga caggtattgc ttcagcgact gcgggagttg gtacagggga cggtatcgta
     4381 cttcgtctga tcttctgggt taccttgact gctcttagta cttggtttgt ttaccgttat
     4441 gcggataaga ttcaaaaaga tccgactaag tcactggttt atagtactcg caaagaagat
     4501 ttgaaacact ttaacgtaga agaatcttca tctgtagaat ctacacttag cagcaaacaa
     4561 aaatcagttc tcttcttatt tgtgttgaca ttcatcttga tggtattgag cttcattcca
     4621 tggacagacc ttggcgttac catttttgat gactttaatg cttggttgac tggtcttcca
     4681 gttattggca atattgtcgg ttcatctact tctgcactag gtacttggta cttcccagaa
     4741 ggcgcaatgc tctttgcctt tatgggtatc ctgattggtg ttatttatgg tcttaaagaa
     4801 gataagatta tctcttcctt catgaatggt gctgctgact tgctcagtgt tgccttgatc
     4861 gtagcgattg ctcgtggtat tcaagttatc atgaacgacg gtatgattac cgatacaatc
     4921 ctcaactggg gtaaagaagg cttgagcggt ctatcttcac aagtctttat cgttgtaact
     4981 tatatcttct atctacctat gtcattcttg atcccatctt catctggtct tgccagcgca
     5041 actatgggta tcatggctcc acttggagaa tttgtaaatg tccgtcctag cttgattatc
     5101 actgcttacc aatctgcttc aggtgtcttg aacttgattg caccaacatc tggtattgtg
     5161 atgggagctc ttgcacttgg acgtatcaac attggtactt ggtggaaatt catgggcaaa
     5221 ctcgtagtcg ctattattgt agtgaccatc gcccttcttc tccttggaac cttccttcca
     5281 ttcctataaa atagtgagtg aggtgattcc atgaaaatag atataacaaa tcaagttaaa
     5341 gatgaatttc ttatatcatt aaaaaccttg atttcctatc cttcagtact caatgaagga
     5401 gaaaatggaa caccttttgg acaagcaatc caagatgtcc tagaaaaaac tttagagatt
     5461 tgtcgagaca taggtttcac tacctatctt gaccctaaag gttattacgg atatgcagaa
     5521 atcggtcagg gagcagagct tctggccatt ctctgtcatt tggatgttgt tccatcaggt
     5581 gatgaagcag attggcagac accgccattt gaagcaacta tcaaagacgg ctgggtattc
     5641 ggacgtggtg tccaagatga taaaggccct tcgctcgcag ctctctatgc agtaaaaagc
     5701 ttgctggacc aaggtattca gttcaaaaag cgcgtacgct ttatctttgg taccgatgag
     5761 gaaaccctct ggcgctgcat ggcacgctac aataccatcg aagaacaggc cagcatgggc
     5821 tttgcacctg actcatcttt tcctctgacc tatgctgaaa aagggcttct acaggtcaaa
     5881 cttcatggcc ctggatcgga tcaactagag cttgaagtag gaggcgcctt taacgttgta
     5941 ccagacaagg ccaactacca aggtcccctc tatgaacagg tttgtaacga tctcaaagaa
     6001 gctggttatg attaccaatc cactgaacaa accgtaacgg ttctcggagt gccaaagcat
     6061 gctaaggatg ctagtcaagg tatcaatgct gtcatccgac tagctaccat tcttgctcct
     6121 ctccaagaac accctgctct cagctttctt gcaacacaag caggtcaaga cggcacagga
     6181 agacaaatct ttggtgatat agcagatgaa ccttctggtc acctatcctt taatgtcgca
     6241 ggtctcatga tcaatcatga acgttctgaa atccgtattg acattcggac tcctgtctta
     6301 gctgacaagg aagaactagt aaagttgctt acaagatgtg cacaaaacta ccaactccgc
     6361 tacgaagagt ttgactatct agcgcctcta tacgtcgcaa aagacagtaa actcgttagc
     6421 acactgatgc aaatctacca agaaaagact ggcgataaca gtcctgctat ttcatccggt
     6481 ggtgccactt ttgctcgcac catgccaaat tgtgtagcct tcggcgcctt attcccagga
     6541 gcgaagcaga cagaacatca ggcaaatgaa tgtgccgttc tagaagattt gtaccgtgct
     6601 atggatattt atgccgaagc cgtctatcga cttgcaactt aatcaggcaa ctgtttctac
     6661 caaaaaaaat cgaccgatta atgaactgca ccccaaaagt tagacagaat aaatctaact
     6721 tttggggtgt tttattatga agataaagtt cagatctatg aactaagaaa gcaaggacaa
     6781 agcttcaaac agctttcaaa aagatttgtg tgtatgtttc tggtctaaag tcatctgaat
     6841 ctttgagatg agctttataa atcgcttttt tcagtttttg cactggtgtt tcgataaact
     6901 caaacttttt agccgtggta ttgcctgatt ttatagtata ttgaaactag aatagtacac
     6961 ctctacttct aaaacatttt tagaaatcga tttgactgtc ctgaacgatt tgtcctgttc
     7021 ttatttcatt ttactatatt tgagccactt cgtctttaac ggctttattc ataagctctt
     7081 gtaatttttc tttactatca attacttctg attttccgtt gtaatttatt gtaataggtt
     7141 ttaacttacc taatttctcg acacgctcat taatttgatc ttttttgaag gctgcatatg
     7201 tttttcctaa gattttttcg aaaatatatt tatcagatag cggtttgtct tcttcagctt
     7261 ggtttttgta ttaatttgaa acgtaaggaa caaatccttc atagtaacct aatgctccca
     7321 taagttcaaa agcttgtttt ctaattcaaa ccattgcaaa tcagatttca gcttttcaga
     7381 taaatcctgc tcatccaaat aatgacttaa aattagtgct gaactcgttt ctgtatcctg
     7441 tacaggctga gcacccatac cagcaaaaaa taaactcgtt cctagcaaga ccgaacaagc
     7501 tcctattgca tatttcctca aagaaaaacg ctgctttctc tcaaattgaa attctttcat
     7561 cccatctccc atcattcatt attactgtat attttgtata tcagaaatag tttgtattca
     7621 caaatctttc tagttattcc cttatcattc ctaattaagg gagataacat acaataattt
     7681 ttagttaaat gtatatcgat gttttttgtt tttcttaata aacgcaatac aaaaagagcc
     7741 tgttgccaag ctctttgtac tcaatgaaaa tcaaatagca aactaggaaa ctagccacag
     7801 gttgctcaaa acaccgtttt gaggtagtag ataagactga cgaagtcagc tcaaaacact
     7861 gttttgaggt tgtggataga actgacgaag tcagctcaaa acactgtttt gaggttgcag
     7921 atagaactaa cgaagtcagc tcaaaacact gttttgaggt tgcagataga actgacgaag
     7981 tcagctcaaa tatacggcaa ggcgacgctg acgtggtttg aagagatttt cgaagagtat
     8041 tagtctatta tttcttctca gcgcgaaggg ctgacaagat ttgtgttcgg atatcatcca
     8101 caccatttgg cgtatttggt aaaaagatgg tttgatttcc tttagaggca aaggtattca
     8161 aggtatccaa atactggttg gtcaagagga tagacatgat ttgttcttct gtcatgccaa
     8221 cattggcttc cttgagttcg gtgatagact ctgccaatcc atccacaatc gccttacgtt
     8281 gttgggcaat ccccacacca tgaaggcggt ctttttctgc ttcggcttca gctgcagtga
     8341 caattttaat cttgtcagct tccgccaatt cttgtgctgc gacccgctta cgttgcgccg
     8401 cattgatttc attcatggat tgcttaactt ctgcatccgg ttcgaccttg gtaatcaagg
     8461 ttttcacgat aatgtagccg taagtggtca tttcttctgc tacttggtgt tgaacctcaa
     8521 gggcaatctc atcttttttc tcaaacaatt catccaaggt taattttgga acagaagagc
     8581 gaagagcgtc ttcgatataa gatttaatct gagattctgg acgtatgagt ttatagtaag
     8641 catctgtcac gctctgctcg ttgacacggt actgagtcgc tacattcatc ataacgaaca
     8701 cattgtcctt ggtcttagtc tcaaccacaa tatcactttg caacaagcgc aactgaatcc
     8761 gtgctgcaat cgagtcaatc ccaaaaggca agcgaatatg aataccgcta ttagcaacct
     8821 tttggtattt cccaaagcgt tcaataatcg ccaccgactg ctgacgaacc acataaactg
     8881 tactcagtgt gactatcacc aataggagca cacaaacaat cagaaaaatc atgaaaaata
     8941 ttgccataat ggaacctcca caagtatttt tctagtatta tagcacattt aaagaaggct
     9001 gtgccgtttt tactgcgatt tttcctgaaa tgtcaataat tagaggtgaa ttgtcctatt
     9061 gtcgtccaat ctcttgctaa aataactctt tataaaaggc aatcgtttct tctaaggttg
     9121 gcataaatgg atttcctggt gcgcaggcat caatcaaggc attcttagaa aggtattcaa
     9181 agtcgaaatc tttttcttca ataccaagtt cagtcagttt cttaggaata cctactgtct
     9241 cagaaagctt ctcaatctca gcaatcgcat aatcggcaca ctcttgatct gatttacctt
     9301 ccacatgaag gcccaaggct ttggcaacat tgcggaaagc ttctggtaca cgtttagcat
     9361 tttcacgttc tataactggt agcaacatgg cacagcacac gccatgtggc aaattatata
     9421 ctgcaccgag ttggtgagcc attgagtgaa cataaccaag accagcatta ttaaagctca
     9481 taccaccaag gaagatggca ttgaccatac cttcacgcgc ttcaatatca tgtccatttt
     9541 ctacagcacg aggaagatat tccttgataa gctcaatagc accaatagac agtttcttgg
     9601 ttacatcata agcaccaggt gttaccaaag cttcaacagc gtgagtcaga gcatccatac
     9661 cagtagcagc agtcaggcct ttaggtttgg aaagcatgag ttccgggtca ttaacagaga
     9721 taagagcaag gctattcttg tcaaccatta ccatcttaac cttgcgttct tcatcagtaa
     9781 tcacatagtt aatagtgatt tctgcagaag ttcctgccgt tgtattgata gccacaactg
     9841 gcaagccttt tttagcagac ttatgaagcc cttcataatc ctgtggtttt ccaccatttg
     9901 tagccatgat agaaatacaa ctagctgcat cctgtggaga acccccgcca agactgatga
     9961 taaagtcaca gccatgttct tgcaaagcag ctaccccatc tgtgacattt ttacaagtag
    10021 ggtttggctc tacatcgcta aagatgacat attcgattcc ttctgtatcc agtggtttta
    10081 aaaccttagg caaaatatca ctgccttcga tgtacttatc tgtaaccaaa agtgcctttt
    10141 tatagccaag ttccttgata taaggaccca cttcatttac aacaccttta ccaataaggt
    10201 tgactgccgg aacataaaat gtagccatat aattttcctc ctgcgcctcc gcgctatcta
    10261 tttaacatca ttatattact ttttgtattc gttttcaatt atttgtgaat tgtttaacaa
    10321 atctttttaa aaggatagga gggacgtaag ttcacctcct atataaacaa gttttattta
    10381 tgtagtggtc ccaacaactg acatgcacga tagtctgctg attctagatc ttctgttcca
    10441 aataaggacc aatttttagg tctaaagata tcttcctcag gtacattatg catattgaca
    10501 ggaattctca acatagaagc caaggtaatc aagtctgctc caatgtgtcc atatgttatg
    10561 gctccgtgat tagctcccca attattcatg acgtcataga cagacttgaa agcacctttt
    10621 cctgtcaaac gtggagcaaa ccaagtagtt ggccatcctg gatctgtacg attatctaaa
    10681 gtatggtgaa catcttcagg aagttcaagt gtataacctt ctgcaatttg tagcactgga
    10741 ccaacccctt ttagaagatt gagacgtacc attgttactg gcatatcccc cttcgtcaag
    10801 aaacgagttg agaatcctcc tccacggaag tattcgcggt ttgctggtgg gaagtctgta
    10861 ttttcaagca tagcctgcac ttcactttct tccaactccc agaatggttt cataacaggt
    10921 ttgccatctc gagtagcttg acctgtacca tccaatgtac aagaaccaga gttgattaga
    10981 tgtaagaagc cagctgcagc acgaccctct aaagtatgtc ccgttacacg tttaacagcc
    11041 tctgggctcc aataagtacg cacatcagca aagatttgtg gagtatttgt taatagataa
    11101 ttaaagagca tagacacacc atttagtgaa tcattctctg tcgcaaatac aaatggtttt
    11161 cgaataccat tccagtcaaa ctgagtattg aggaaagttt ccataaagtc cccatttggg
    11221 aaatgatctg tccactggcg ttgaccttgg aaaccagcta ctaaagcatg gtgaccaact
    11281 gcttcttcct caaaaccaag ctcagcaagt cttgggttac caaccattaa gtcacgtcca
    11341 atcatgaaca tcttaataac aaattcccat tgtctatctt tttcttcacg gcttaaaaca
    11401 aggtcttcac ggttatggtc gaatccttct tttacgtttt ctttcaccca tttgagcgca
    11461 cgttcgaact cttcagggtc gtaaatacta cggtccatac ggcgcgtgaa ctccgtcata
    11521 tctaccgatt catttcgcat tcctaggtat tcttggaaga aatccggatt tacaatagaa
    11581 ccaccaatcc ccatcgaaac actacccatt gataggtaag cagtgtctct catcaagcca
    11641 gttgcaagag ctgcacgcgc atagcgtaaa agtttttctt tgacatcttc tggaatatct
    11701 gtgtcactag cttcctgaac atctcttcca taaatcccaa aggctggaat ccctttttga
    11761 gcatgtgaag ctagtacagc tgcaagatag acagctcctg ggcgttctgt cccattaaat
    11821 cccaaaatag catgaggaat atctggagac atatccatag tttcactacc ataacaccag
    11881 catggtgtaa ctgtaattgt tgcgcaaaca tttgattttt taaacaactc atgggaagct
    11941 gcagcctctg gtacacggcc aatagtagat ggagaaatca cgcattccac aggttcccca
    12001 tctggatatt tcaatgtgct tgaaatcaaa tctgccacac ttttagccat gttcattgtt
    12061 tgcacttcaa gtgattcgcg tacaccttga cgacgaccat caatagtcgg acgaatccca
    12121 atacgtggat gttgaatcat actattttcc tcttattttt ctgtttcttt catactacca
    12181 tctccattat atttacggta gcctgggtga taatttttaa aattacttat aagtataaac
    12241 ctctacctct gctaaactta aaggaattcc tgcgtttctc aaagaaattc taaccttttt
    12301 tcccttcagt ccttttaagt ctatggatag attattgctt tctaaactgt caatatgttg
    12361 tgtaaaaact tcataatcat ttgaatcata tataataata tcaaaatttg ataatctctg
    12421 aatttcagca tctgttctat tataaataat taacttttct aattcttcgg tttgtgctaa
    12481 atctatctcc caccatgaag gagaatcttc tttggtatga gtcactgaat gatgaccata
    12541 atctccgttt ttatttccat caactgctag acgagaaaaa ccattgtaat ccgttgaact
    12601 ctgtcgagtt tcttttgtta atgcaatatt agaaagtttc ttcggagttc tctttgaacc
    12661 atagacttca acttccgcta aacttaacgg aatagctgct gattttaatt ctattttgac
    12721 tagcttagcc cctacagaag gtacttttaa agataacaaa ccatcaacta ctttatcaaa
    12781 atgtcttcta aaaacttctt ctcctgatga agatagaaaa ataacatcaa aattagataa
    12841 acgctgaggt tcggcatctg ttctattata aatatcaact ttagaaaccg taaactcttc
    12901 tcccagatcg acctgccacc aagcgttaga atctgcctta gtatgagtca ccgaatgatg
    12961 tccgtaatct ccatctttat taccatcaac tgctaaagca gctacaccct cataattggt
    13021 tgaactttga gtagctacct tatttgtaga gactacttta tcttctgtta ttttatctat
    13081 atcctcttca gattgcttac catctgattc tctaaaaacc tctacttctg ctaaactcaa
    13141 aggcactcca ctcgttagta atttaacttt aatatacctt gctccttttt ctttgaaatc
    13201 tagactaaca gattcacccg acaaattatt aacatgtttc ttagcaactt cgtttctatt
    13261 attgtcatat agaatcacat caaaattaga tagacgttga gtctcagcat ctgtgcgatt
    13321 ataaatttta acaagcccaa ctttatccat ttttttcaaa tcgacttccc accaagaggg
    13381 attatctgcc ctagtgtgtg ttaccgaacc agagttaaaa ttaccatttc tgttaccatc
    13441 cacagctcta ttagggtctc cattgtaatc tacagaactt tgtgttgttg gttttgcata
    13501 tgctatattc aaattaccat cattaaattt atctggtgtt gggaaagaga ctggacctgt
    13561 cccctcagta tttatagaca tctctaccat tccattatga ttaaccgtaa tggtataaac
    13621 atgggtattc tcatcccaat tttctgtata agtataatga tttttatcgc cacttatatt
    13681 tatctgaggt ttatgaccag tatgcccttt taatgtaagt gttgttgtcc taaagtcatt
    13741 atctacagga ttgatggaat aatttttgct tatccagttc gctaactcta attcttcttt
    13801 cttccaactt tttgatgcat caaaatttcc tgataatgcc cacatagctg tcttatctgt
    13861 cctgtaatta ttcaataaaa tatgtaaatt atttggattt tctttaacaa cagcgtaagt
    13921 atgtggcgtc aaatctaacg ataacgactt tgtattatta gtatacatag gcaacattac
    13981 ttgctgattt ttattgatat tagcattact attataaata taccaggaat tacctacacg
    14041 ctgagcatac ccatctcctt cataaagttt tggataaagc gagtttaaat agttgacttt
    14101 actagacaat tcctcactat ttttagtcaa aatttttgca ttagggaata tagatgaaat
    14161 cttttcctta tcaattttct catgtattac aggaagaata tgatatctcc cattattata
    14221 taaaggcatt gtttcatcat tcgaataaag tccttgataa aatccgttta atgaactaat
    14281 cctaccttct ccattccaaa atacagcttt tgttctattt acaacttctt ccttacttgg
    14341 agctggattt tgtatagcat gtctaaagaa aggaataata cctttagtaa atgctggagt
    14401 tggtacatca tttgtcataa atgtatacgc ggcacattcg aaattataaa ctgtgcctcc
    14461 cccagtatat acattcatca tttccatagc aatcattgat tctggttccg atgcatagga
    14521 tctcatatcc ctagctcttc cagtttcaaa tgtgtttgta taatgttttt cccaccattt
    14581 ccatgtatct gttgatgagc cccagttatc acataagcca ctcaaccaaa atccactaac
    14641 gatagaatct gtacccgcat catctcttat tggcgtattt ttagttgcca acaccaaatt
    14701 tttatgatat ttttgactcg cttcaaagaa tgtcggatca ttcataatag tttcccagaa
    14761 ccatttttca tgatcatgcc agataaaatg cgctccatat ttggcacaaa cttccaaata
    14821 tttagcacta tgcggagcta actggttatt gtaaatccaa taattctcaa tatttaaaac
    14881 accttttaac acactatact tttggaattg ttcatctaac cactctggag gaactgtatt
    14941 acgctctcca gccgacataa taaccaagaa aactggaatg tttagacttt gagcttcctc
    15001 caacatcttg acataccatt ctctcaattc tttcgtatct cgtggaatac aacttgttgg
    15061 tttacagact tttgcaggat gaagttcaat cgctgcatat ggctttacat cttcaggaat
    15121 agcttcccat gctcccttca atgtgttccc ccaccataat ccgttaccat tatcattgcc
    15181 atacaacgga gaaagcaaca atggcgattc attattaatc gtcgttctca tttgaacacg
    15241 attatcagcc ttaactcctg ctaacaatcc aaaacataac atcccaatac atacaaacaa
    15301 tattgtgatt aattttcttt ttattttttc tttattcatg acttatacct aaaaatttaa
    15361 ctcaataatt ttttctgaat taaataccaa ttcaatattt tgtacatcag tatttttgcc
    15421 atatattctt actcttactt tttgatcttt gtttcctcct tccaatttta ggaatgttat
    15481 atccccattt ttccaagcaa acgatacctt atatcctcct cttactctga aacctttcac
    15541 ttctccttct gaccaagcag aaggtaaagc tggaattagt gataaccaat tatgatggct
    15601 ctgtactaat aattcacaaa ttccactcac caaacctaaa ttaccatcaa tttgaaatgg
    15661 tggatggtca agaaataaat tgccaagagt cgcattattt aacaaaccat taatctggtt
    15721 ataagcaggt tcaccttgat atagtctcgc aaaaaaatga atcagccatg cagcactcca
    15781 acctgtttgt gtactagcat gcaaaccact tactaaccaa ttattaatcg cttgctccct
    15841 ctcctgtgaa gataaaaaat tagcgtttga taatctccta ttgatagtga ttttagctgc
    15901 ttctgctaat tccggagttt tatgaatatc aatctcatta taaggataaa gcccaaatag
    15961 aggtgaaatg tgtctatgcc caggctctac ttcttcataa tcttctaacc attcttggat
    16021 ttgcccatta ctacctattt ttgttttagg tagtttcttt tttaactcct tcacacgact
    16081 aataaaatcc gaattgtctc ctaattgttt tgcaatgcca atgcatgaat cacaaaaata
    16141 tcttagaatt tgattatcaa ttgtagatga tagacaagca tttccttcaa taccattttt
    16201 taagcgatat ttattttccg gtgagacact tggacctgtc atcaagtagc catccacctc
    16261 aaataaataa tcttcaaaga aaagaaatgc ttcttttatc atttcaaaat gttccgtaag
    16321 aatacgctca tcttggaaat ataaatagtg ttcccaaata tgagtacata accatggaat
    16381 agttaatacc caaattgcag cccccatggc atgagattgg ggagccgtat cgccaaaacc
    16441 atccgtatta tgatgtgctg taaaacctct agctccatac attttcttag cggttagtct
    16501 tcccggttct ctcattcttt cgagcatatc aaataatgga tattctactt ctggtaaatc
    16561 acatggacct accatccaat aattcatttg agtattaata ttaatcgtat atttagaacc
    16621 ccaaattgga tttaattcat cacaccatat tccttgaaga ttggcaggta aaccattcgg
    16681 ttgactagac gatattaaca gatatcttcc ataatgaaat aacaagttag tcaagtagtt
    16741 actatacttt ttagtgtttt caagaagtag attcgttgga atgctaagac aatctttact
    16801 atagtctagt ttaaaatcaa ctctattaaa ttgctcctga tattttttta catgttcatc
    16861 tttttctgta aagtaatcaa tactactaaa ttctccctga agagaagaaa tatctatatt
    16921 tccccaataa tccgtcattg atttgagata aagaaatacc tctgtagcat tccgaataac
    16981 tattgtctct cccaatacac ttacttcacc atccgtaacc ttagaatgac atactacttt
    17041 aaactgaaca ccttttctac ctccagcaga ggccgacatt aaaattgtac ttgaatccag
    17101 tttagatact tcatcattaa accgtttatt tctacccaaa ttaatgttta aatttaatgt
    17161 gttttgaact gatgacacta tacggcaaca taaaatattc ttattaaaac tcgtaaaata
    17221 ttctcttttt atttgtaaat tacaactatt aggctcaaac acaacattag aaatagctgt
    17281 atctaaatct agctctcttt catacaatga aagagcacaa gactgaatat ctatatgctc
    17341 aatgtaaagt tccccaagta attcatagtg gctttgatct cttggggtag caaacattgt
    17401 taactttatc aattcttcgg ctttctgaat ttctccatct aaaagatatt cccgaatttt
    17461 tttaagatgc aatagtgagt ctggattatt tctatctgac tttcctctat accaaatagt
    17521 ctcatcgttt agttgaatac attcttttgt agctgaacca taaatcatac cacctaaatg
    17581 accgttccca atcggcaagg cttcattcca atttgaggca gctttcttat accataattt
    17641 cactctttac ctctcttcca aaatggaggt gatatcccaa ataccacctc cacactttct
    17701 actacttcaa aattcctaaa acaactagga taactccaat agccataaca ctaaagatta
    17761 gtacaaccgg attctttcct tttctaatta atttacacat taataaagtc aaagccatag
    17821 ggataaatcc tggaattaat tttgtaatca tttcttgaac agaaataaca agttccccct
    17881 gcttaaattc taatccaaaa ttaataccaa ccattgatgg aatcaaacca cccactagta
    17941 ctagccctaa tgctgtcgcc atactcgtaa cgcgattcaa tgttcctttt gtattatttt
    18001 cttggataag actagaaccc ttagtatacc catatttcaa accgaaatat ttaacaggaa
    18061 tattaataat attaaacaat attaaggcga taaagatacc taaagcaccg ccgtctttag
    18121 aataagacgc accaatacta aaacagatag gaactagtgt cagccagaac aaactatctc
    18181 ctagaccagc cagtggcccc atcaagccaa ctttaatacc agtaattgaa gctgcacctt
    18241 catttccttc ttgttcttcc atagcggaag taactccaag aataaatggt gccgctgttt
    18301 gatgagtatt gaaaaactca aggtgacgtt tcattgcagg agaagctgaa tctttgtctg
    18361 ggtatagttt tttcaatact ggaagaattg tatatagaaa acctaggttc tgcattctct
    18421 catagttcca tgaagaacca tacataaaac ttcgtttgaa aactttttta agttcaactt
    18481 tagttacttt attattcatc atagatcaag atcctcctca acatcactac ttgaagttgt
    18541 ttcttgtggt ttacttccaa tcatatcgta gaagtatgct accgcaatac caactagtga
    18601 gatcccaatg gttgacattc ctccatacac agaacaaatg aatccaatca acaagaatac
    18661 ccacatattt ttcttgagca tcatatttaa aagcatacca aaaccaattg aaggtagtaa
    18721 agcacctgca gagttaagtc cctgcataac gattggtgga accttagcaa acaagtctgc
    18781 cacatattcc cctccaagca taatagctag gaaaattggt acaattttaa ataacgtgat
    18841 taaaaccaaa cttgaataat gataaaaacc gatctttgaa taatctccgt ttttagcatc
    18901 attatcagct tttttcacaa aataaacatc taaagttttt gcaagaacat ccaactgttg
    18961 ggtagcaact gcaattggaa ctgctatagc gataccagca gtttctcctt gaccagataa
    19021 aataccaaat gcagtaccaa taatcgcacc tgaaatagta tctggtggag tgtaacctcc
    19081 aatacctgtt acaccgagcc aagttaattc aagagctgaa ccaataagaa caccttgggt
    19141 gaaatctcct aagactaagc caaccattgc acttgtaact aacggacggt gaatcataaa
    19201 taacggtcca ttatagtcaa tagtggcaat aatcccaact aatgtcacca gtaatgcttg
    19261 tgtgaatagc atatactgta cctccttaaa ttatttttat aaaaattttt ccaacattgt
    19321 aactttatca ttaggaacca tttgaatttc aactctcgtt cccttatcaa ctattttctt
    19381 aaaataatat ttgtcttctt cacctagagc tacacctccc gttacctttt cattttcctc
    19441 cgttttactc atctgtccta cattgaggta ctccaatttt aaatttcctt caatagaatc
    19501 atacacatct tttggatttg tatatatcag cattgttctc ttttttattg gcacagagtt
    19561 taaaacttcc acaaaccgtt ttacactaaa gaaaacaatt tttaaaccta ccggtgcaga
    19621 aatctttaaa atagattgtc gtgttttatc ttctgagatg cgatcattaa caatgataac
    19681 ttgctcaata tcatactttt ttagccacgt agtgacaact tgaccatgta ccagacggtc
    19741 atcaatacga acaaactcta tagacataaa tcctcttcct cctctacgtt aagttgttgt
    19801 ttaacattaa acaaactatt ttgagcattt tgaacaattt cttctaaatt gatttttgaa
    19861 tcataacttg atattaattc cactaggaga gggatattaa accctgttac aatatcaact
    19921 gaatccaaat ttaaaaaccg tgacaaagcc acattattag gacttcctcc aatcaagtca
    19981 gtcaaaacga taacctcttg atttgagtct aacagttcat ttacttgatt tttaaaataa
    20041 tgttcaaact ctacaatatt ctctccaggc atcaaaccta atgtcctaac tctatcattt
    20101 gttgtctcac caacaaacat ttctgcggtc atgagagctc cgctagcaaa attaccatga
    20161 ctggcaacta aaattcctct attaaacatt ttctctttca acaactactc ctttcttaag
    20221 gataatatta gcataaagtg aagtttctcc tgtagcaaca atagcataag ctttcttact
    20281 acgttcataa aagtcttctc ttctaagata agtaatcgtt ttaagatctg taccatgacc
    20341 ttcaatcatc tgtctatagg taccccatat cttaggaata tcatcacccg aaacaacgtt
    20401 cataaactga attgaactat cgacgtaact atctaatggc attaaataca gaatggaatc
    20461 taataattct ggaatattta cgccatcaca acgaattagc ttatttgcac atgaggcaga
    20521 aggataattc gcgtcagcta atactatttc atctccatgt cccatttcca ttaaagtctt
    20581 caataaatct ggagaaatat ttttcggtat atgttttaac attaatatcc tccctatttt
    20641 ctctgaccgt atgttttaaa tttttctgcc attaaatcca tctcctcatc aggaagaact
    20701 actggctctc caaaattctt agctaaacaa taaatttttg cacaatattc aacttcttca
    20761 acaatattaa atgcattcaa taaattttgt gcacctgcta aaattccatg attcgctagt
    20821 aaaactgctc tacgaccttc cattgcttta gctgcattca cagccaattc tttcgtacca
    20881 tatgttgcat actcagctac ccgaacatct ttccctgcca ctgcaatcat ataatgacta
    20941 gccggaagtg gttctctgag acaagctaat actgttgcat aagttgtatg agcatggata
    21001 attgcatcga tatcatcacg agtttgatat tgaatcaaat gcatatacca ttcgctagat
    21061 ggcaagcgtt ctccctctac aacatttcca ttaatatcca tcactacaat atcggattct
    21121 ttgatttcaa agaaatcaat acccgacggg gtaattgcca tcaattgttt ttcacgatcg
    21181 aaaacactga gattcccacc tgttcctttc gtcaaatctg tttctactag cttcttgcca
    21241 tacttaatta attcttgttt tacatctgac attcttgtcc tcctcatatc aaaacactaa
    21301 atatttttca attcaccaat tatcccccct tcttgtacaa aacttaagaa gggggaggga
    21361 ggcaaattta caaactctca atctttttaa ttcttttggg agtaataact ctctaattgt
    21421 agaaactcct caataacatg gtgagcttct tccatccctt ttaattgtcc catagctatg
    21481 agctgcacaa caatattccc cacagctgta gcctcagtca aacctgtaag aacctcttta
    21541 ccagttctat cagcaatcat ttggttaaag taactggctc tagcacctcc tccaatcaca
    21601 tatatcctct tataaacctt atgagttagt tcttctagta actctatcgc tttcctatac
    21661 gtttcagcta ggctttcata aacaatctta aatagttgtc catctgtcca ctctctagtt
    21721 tcatgatgat aagctagata ttctgtcaaa gtcttgtgca tatcagattc tgttgcaaat
    21781 tcagttgatt cagtatcaat cagaggaaga ttttcttttt ctttctccac cattgtccta
    21841 atatcatcaa aagaataggc tttccctctt cgttcaaatg aacgtcttag ttcctctatg
    21901 atccacaacc ctgtacaatt cttcagaaat gtaatcactc catctttacc gacttcattt
    21961 gtaaatccat aactgaagga ttcggtagta agaagcggtg aagtaagttc cactccaacc
    22021 aaagaccaag tacctgatga aataaataaa ctaccttctg tcttaggtac tgagacaatc
    22081 gcgcttgctg tatcatgact acaaacattc acaacaggaa tatcgcctaa accatactcc
    22141 ttttttatcc ttccaagaac atttccctct gaaacaatct caggaagtaa agatgaatcc
    22201 aattcaaata gttttaagat attctgattc cagttttgac tcctaggatc aaataattga
    22261 gttgttgaag caatgctttt ttctgtagca aacttacctg tcaagagata attaaacaaa
    22321 tctggcatta aaagaatctt attggtctta tagaaagagt caggagattc ttgacgtgcc
    22381 ttaaagagtt gaaacaaggt atttatctcc ataatctgat ttcctgtctc tgaatacagt
    22441 ttttctaatt cagtcatttc agatatttcc tttaacactc cctttgttct ttcatcacga
    22501 taatgaacag gttgtaataa cagcttacct tcattatcaa ccagtccaaa atcaactccc
    22561 catgtgtcaa taccgataga taaaatcttg taactagtat tagccaagcg gatactttca
    22621 agaattttag ctagtagaaa gtcaatatcc caagataaat gcccttttac tctaataggt
    22681 agattagaaa agcgatttat ttcttccatt actagtttat tttcagaaag gtaaccaacg
    22741 attgctcttc cagaagtcgc acctaaatct attgctaaaa ccgcttccat ttttcttctc
    22801 tcttcgtcct tgattaactt tattataatc cccacgtttt attttttcaa tatgtgaacg
    22861 cttacttgaa taatgttcaa atgttaaccc aatattcata aaaataattc aaattcctcc
    22921 aatttgtgct ataatatagt tgaaatgata aattaggagg agcttatgat aaaagatgaa
    22981 cgtgtacttg aattgattga aatcatcaaa aagaaaaaaa gaattgccgt aaaagagctg
    23041 gcagaaatca ctttctccag cacaagtacc ttacgtcgtg atttaatttt cttagaaaat
    23101 caaggtctta tcaaaagaaa gcacggatac gtgaccctgt cctctatgaa cacaattgaa
    23161 ctttctcatc aaatacggga aggagaaagt actaggcaaa aaagactaat cgctagtctc
    23221 gctaaagact ttattcggtc aggcatgtgt atttatcttg attccagtac gactgtctac
    23281 gaactctgtc cctatctttc tgaacttgat aatttgatta tttttacaaa tggtttacat
    23341 actgcacaaa ccctatctga aactgttaaa gatagctcca aaatctttat cacatctggc
    23401 gaggtcaaac atcaatcctg ttccgtagtc aactatgata aggaaaattc tttattagat
    23461 cattttaata tcgatttagc attttgttca gcaagaggta ttgatgacca atatgtttat
    23521 gaagcttctc tcagccaagc tatttcaaaa aagaatatta ttgacaaagc ccatgaaacc
    23581 atcttactga ttgatagttc taaattttac aagactggat tttttaaaat taatcccctc
    23641 tccaaataca caacctttat ttctgacacc ttgccagacc aaaaattatt agacgcagtc
    23701 gaattatttg atggagaatg ggtttctgat attcaatgaa aatcaaatag gaagctagtc
    23761 tcagcttgct caaagtacag atttgaaaac tgatgaagtc agctcgaaac actgttttga
    23821 ggttgtagat agaactgatg aagtcagtca catatatacg gaaagacgaa gctgacatga
    23881 tgtgaagaga ttttcgaaga gtattcgttc tgtctgctga ctgatttaca aatattcacc
    23941 acatgacatt attttcttag caaccccaaa aacagaggct ggacaaaaac tcaatttcag
    24001 gagaaaatgg agtaaacctt cccacaataa aacgcatagt atcaagtttt tgcacacctg
    24061 atattatgcg tttttctgat tttaaagact ttgtcgccta gaccctatat ttcaagaatt
    24121 aagtgacctt gatgatctaa atttattgtg gtatactatt tttgtcatcg gttaccgatt
    24181 acgttcctat gggttcgtga gaggaggtga aactaatgag ccttatcata gagctcgctc
    24241 ttactattat agcggatgtt atagctggaa ttatcttgta tttcgtctgc agatggctag
    24301 atagtaagga gtagaccgac tgactagcct ataaacaccc gttaaatcgc taagatacgt
    24361 caaaaaagcc cttaactacg gccatagtta ggggctttgg tgttctaatg aaccttatca
    24421 attaactaca ttttagcata taatctacaa tagttcaagc attaagtgag gttgatgatc
    24481 taaatttagt gtggtatact atttttgtca tcggttaccg attacgttcc tatgggttcg
    24541 tgagaggagg tgaaactaat gagccttata ccagagcttg ctcttactat tatagcagat
    24601 gttatagctg gaattatctt gtatttcgtc tgcaaatggc tagatggtaa gaagtagacc
    24661 gactgactag cctataaaca cccattaaat cgctaagata cgtcaaaaaa gcccttaact
    24721 atggcactag ttaggggctt tggtgttcta atgaacctta tacactaact acattctagc
    24781 atataagtcc agatatttca agagttttat ttattttttc aggttccctt agttctgaaa
    24841 ggtctataat gaagttagcc atctagtatc aaaaaaccga ctagctctta tgaactagtc
    24901 gatttctcat caatgcgcca acatttcttg ggcgatttct tggccagata ggttatctgg
    24961 gtagtaggtt ggccagttgt ccatttcttc aaagagggct tcttggcttg tgcctccaaa
    25021 gaagatatgg aaatgttctg ccttaactgg ggcgatattg tggtcactaa actgaacata
    25081 cttgaattgt ccagcgtcag catctgtggc ttcaaagagg aaacgcacgc cacgattgcc
    25141 tttcttgtaa gtcaaaattt tcttaccgac atacttgtaa gtgtatttct tgctttgtcc
    25201 accttgaaca aattccatag tattatcagt aatgttaatc ttagtcacat ctgtctgata
    25261 gccttttgta tagtaagcct tgtactcagc ctgggtcatc ttaccagtca acttagcctt
    25321 gtagtcaaag acttggtcaa acgtgccgtc ttcaaggaaa ggataaactg attgccagtt
    25381 acctgcatag tcactcaagg tgcggtcctt gacagctgca tcctcgaagt aaccattttg
    25441 gactgtcttg gtatcctctg ccttttcagg ttcgattgct gggccttctt ggtctgttgt
    25501 ttgtttcaaa gccttgaggt ttttctccat cacggaaatg tagttttctc cagccttggt
    25561 gtcctcttct gtcagacttt ctaaaggatt gaggacatca gttttgacac ctgcttcttt
    25621 tgaaagtgtg ttagcaaggg cttgtgaggc attttcttca aaatagatat aggcgatttt
    25681 atttttcttg acatactctg tcaattctgc caagcgagca gctgatggct ctgcatctgg
    25741 agaaaggcct gagattgcga cttgtttgag tccatagtcc aaggcaagat agttaaaggc
    25801 tgcgtgttga gtcacaaagc tcttttgttt tgcttgagac aagccttctg cgtaagcctt
    25861 atccaaggct tgcaattttt cgatataggc agctgcattc ttctcaaagg tctctttttt
    25921 atcaggataa tctgctgaca agctgtcgcg aatgtgctct actagtttaa tggcacgaac
    25981 tggtgataag caaacatggg gatcaaactc atggtgatga ccttcttctc catggtcatg
    26041 gtctccctct tcttcctcgc cacctggcaa gagcaacata tcgcctgtcg ccttgatggt
    26101 tttcactttt ttcttatcca aggtatctag caatttaggt acccatgttt ccatgttttc
    26161 attttcataa acgaaggtat ctgcatcttg gattttggca actgccttgg cagatggttc
    26221 gtattcatga ggttctgtcc cagcaccgat taggagttct acattagccg tatctcctgc
    26281 gacttgcttg gtaaattcat agacagggta aaaggttgtc acgatattga gtttaccatc
    26341 tgcctgtttt tgattggaac aagccactaa aaacaaggca catagactgg ctagtaataa
    26401 gctaattttt ttcacgttcg tctcctattt gataaaacgt cttactaaac tgattagtat
    26461 aaagacagtt acaaaaataa tggtaatact tgcacttgca ggtgtttctg catagtagga
    26521 aatgtaaagt cctgctacca ttcccaaaaa gccaatcgca ctggcaagca gcataaccga
    26581 tttaaagttt ttccccagac gcagggcaat actagctggc aagaccataa tggtcgatac
    26641 cagaagagct cctgctgcag gaatcataag ggcaatagcc acccctgtca ccatgttaaa
    26701 aagaatggac atggtacgaa ctggcaagcc atccacaaag gccgtatctt cgtcaaaagt
    26761 taagatatac ataggacgaa gaaagagaaa ggtcaaaatc aaaacaaccg ccgcaatgac
    26821 aaagagggaa atgacctgtt cttcactgat agtcacgatc gaaccaaaga gatattggtc
    26881 caaactcatt gaactcgagc ttttaccctt gctcatgaca atcagagaaa cagccagacc
    26941 tgttgacatg aggatagctg tcccgatttc cataaagctc ttgtaaaccg tacggagata
    27001 ctccagaaag accgccgcaa tcaagacaat ggcaatagta gaaacagttg gagaaatccc
    27061 caaaaccaga ccaaaggcta cacctgaaag tgagacgtgg ctaagggtat cactcatcaa
    27121 actctgacga cgcaagatga ggaaggttcc caataccggt gagaaaagac tcatagcaat
    27181 aaccgccaaa aaggcgcgtt gtataaagtc gtaagataat aaactaagca tggcccacct
    27241 cctggccatt ctcatgaaca ttgaaacaac gccatggcga gtcttggtta cggactagat
    27301 gaatattgcg atccgcataa tccttaactt cttcagggtc atgggtaatc atcaaaacag
    27361 ccttgccatg atgatgggcg ctgtggtgca tgagttcgta aaattcattt ttacttcctg
    27421 catccatccc cgttgtcggc tcgtctagga taaacacatc agggtcagaa gcaaacatac
    27481 gcgcaattac cgctcgctgc ttttgtcccc cagatagaga ccccaagcgt ttgtctcgat
    27541 gttcccacat gccaactgag tccagactag ccttgatatg ctcctcatca tgagcattca
    27601 aacgacggaa ccagcctttt ctcggatagc gacccgactt gacaaattca tagaccgtac
    27661 ttggaaaacc agcattaaaa ctggcaattt gttgaggaag ataggctatt ctcaatttct
    27721 taccttgcgt atttgtcttt gaaatagcca cctttccaat gcgtggttgc agaattccaa
    27781 gactagcctt gatgagcgtc gtcttagccg ctccattttc cccagtcaag gtaacaaatt
    27841 ccccactatc aacacaataa ttgatatgtt caagaacagg ctccttatca taatagaagg
    27901 acaaatcctc taccgtaata tatctcatta tttgatttct cctactaaag cagtcaaaaa
    27961 ccgctgaatc actttttgtt catttggagt aaactgagtc gccacttgtt cataggttaa
    28021 aagtgtatgc tcatggtgat ggtggtgctc ctcagcgatt ggacgagcca agtcagtcaa
    28081 ctgataaaaa atcacacgcg catctttaga atctttagat gtttccaaca tcccttcctt
    28141 gaccaaagac ttaatggcct tggtaactgc cgcctgactg acattgagac gacgggccaa
    28201 ttctgaattt gttaaagatt cctctgacaa gagcataagg atatgctcct gagtattggt
    28261 cagggccacc tcgctagtgc aatgacctat taggatttca tgctgatttt ccgcctgcaa
    28321 aatcacctca ttcaaaaaag cattgatatc ctttgctagc tgtctcatat ctgactcctt
    28381 tccttttaga cttctctttt ttaagagaaa aatactattc tttgacattt tgtttaccag
    28441 ttaattatat cacaagcaaa aaaagagtca agaaaaaacg tgaaaactag tttcattctt
    28501 gaactcttct atattatatt atctattgaa attctttgac atctccatca taagtcgccc
    28561 aatctttgct gaaaaagcgc tcattcagat ggtaagtcgg agctggtgtg ggattggata
    28621 ggaaaggatc aactgccttg tcaaaagcca accaacccaa ccaaccaagg tgaatggtgt
    28681 ccttcataaa gaaaggctcc ccgccgtcct tagaaaaatc tgctatattg gtaaaacctt
    28741 gactttctaa ctggtagcga atcttctgca ccgtttgttg gtacatatcc tctcgtagac
    28801 cagcatagtc catccatttt ttattaacag gtggaatgat aaaaatcggg tttaccttag
    28861 atttagaaaa ctgtgttaaa accaactgca agtcattata ctctggcgac ttgagatagg
    28921 taaagctttt ctgagaatcc tttaatttct tcaaatcctt cttgatctgc tcattataga
    28981 aataattttc cattcccatc tcattattgg aagtattttt ttcagcatct gctttgacaa
    29041 catcttctat tgcttgataa gaaaactggt ctggcaagat ttttaaatac ttagctacat
    29101 gcttatcgta gttaacatag cctctaaccg aaaactgacc aaaaaaggag gcttggcgtt
    29161 cattaaaacg agccaataat tcaatcattt cattgtctgc tgtcgacaat tcttctttac
    29221 ttgccaactt ctgaaccagg tccttcatag ctacgtttgg gaactgttgc agtaagcgag
    29281 tcgctgcata ttgactagcc tgatccccag attgatgttt cagaaaacta gtcaactggt
    29341 ctccattaaa atactgctgg aaggctgctg gatcatagcc atttttactg aaccactgag
    29401 gtgagataac atacacaact tgtttattct ccagctgtgg taacatctgt tgcattccaa
    29461 aatattggtt aagcgatgca gctcccccct gtcctaaaag ataaggacgg taggaacgat
    29521 tgtatttctc agctaatacc gcaggatgag caccgtcaaa acgaagccat tcactagagc
    29581 caaagaaggg aacaaaacgc acatttggat cagatagtgc tctgactttt tgacttcgct
    29641 ccttaaaact atcgatagta gtagccactg ctgaacgctt ttcagctcct agattatgat
    29701 gcatctcagt aggataaaag aaaatgagca gaaaaaccaa caaaccagcg atcaagaccg
    29761 gtccgaagat catccataag cgtttaagca ttttgtagct ccacaatacc agctatgatt
    29821 ttattagctg tattccagtc gtcacgacca aactctgtta cagggacacg aatgtcaaaa
    29881 cggttctcaa tctccacaat caactcaacc gttcccatac tatccaagac acctgcatca
    29941 aaaagatctt catccatcat gtcagaaaca tcttccataa acaactcatc aataatttca
    30001 ataacttctg atttgatatc catattttat ttccttttat tttttaaacc atagattatt
    30061 caagaatcca gaaaagatta agaatgacaa catgacaaca tggaaagtga caaccatgcc
    30121 aagaaactga atccagcgat tctcaggtag ggcagccttc cctgcttttt tccgttcctt
    30181 attgagcgtt tttttcttgc gaacccaggc atcattgatg accaagccta gtccatgaaa
    30241 gagtccatag gcgatatagt accaggtcac gccatgccaa aatcccataa tcagcatatt
    30301 tacaatgtag gccatgcttg aggttacatt acgattttta aagactttct ttctggttaa
    30361 caccatcacc attcgcataa agacaaagtc acggaaccag aaggacagac tcatatgcca
    30421 gcgattccaa aactccttta aatcccttga taaaaagggc ttgttaaagt tgatagggct
    30481 acggattccc atcaagtttg agatggccaa agcaaacata gaataacctg caaagtcaaa
    30541 gaagagctcc agaccaaaag tatacataac tgccaaggca tagagattaa agaagccacc
    30601 tgactgcaag gctaaattct tcagaggagg gagtaaggtc tctcctaaaa catgagctag
    30661 gataaactta tacaaaaagc cccgcatgat atagcggaca gattcatcca gcatatccat
    30721 caactcatct cgctcaggaa tggcctgata attttcatta aatcgcttaa agcgatcgat
    30781 tggaccactc gagaaagttg gcatgaagag aaggaaacgg aggaattccc agagggtaaa
    30841 atccttaatc actccatctc tcagctcgat gataatccca accgaacgaa aggtcaggta
    30901 agaaattccc aagaacccaa gcaaagactg cgttccattg atagctggtt gcaccttgac
    30961 aaagataatc ggaagtaggg acagaaaact aactaagtag aagacccact tgccatcctt
    31021 gctttttcga taatgcttgt agaaaagcag gagcaatatt tcccagcaaa ggtaaatacc
    31081 caaggcagct agttgattgg tctttccacc caccaacatg gtgacaataa agaagagact
    31141 taccaacact tcataccagg caaagcgttt cttgaaaaag agacctataa agatgggcaa
    31201 ggttgcagca atcacataaa caaaatactg aggattgcca tatggctcta aatgaggaag
    31261 ctgttgaaaa aactccatca tctcttattc acctcgttaa tcaatccttt gatgtcaatc
    31321 tttccatttg gagttagtgg caaactgtct cggtaaagga atttagatgg catcatatag
    31381 gacatcatga tgtctgtcag gtcttccttg atggccttgg taatatcgat atctcgctca
    31441 aactgctcac gaacaccgtc ttttaagatg acataagcca atagattttg taccttgtgg
    31501 tccttgttat agcgcggtac tgcgacagca gattcgataa agcgagactt gttgaggttt
    31561 tgagagacat cttctaactc aatgcggtaa ccgttaaact taatctggaa gtccatgcgt
    31621 ccgccgtaga gaagcaagcc ctcatctgtc atggttccca catcgcctgt gtgataggct
    31681 ggcagacctt caaactcaaa gaaggcttct gctgtttttt caggattttt catataacct
    31741 tttgaaacag ctggcccaga aacaatgatt tctccctgtt cgccatttgg cagtttattt
    31801 ccttcctcgt caatgataaa ggttggagaa tcagccttgg tatagccgat tggtaggcgt
    31861 ttgagagtcg ctaacatctc gtctgtcacg gcaactgctg acagagctac tgtcgcttct
    31921 gttgggccgt aagcattgat gatacgggca tttgggaaac gctcgcgcag tttttgagct
    31981 gttttgaccg tcaattcttc accatcaaag tagaaatgcg tgattccagg cattttctca
    32041 ctgttgaagt attcagataa catggccata tctgcaaagg atggtgttga tgtccagata
    32101 gcgattggca atgaaaagat agccgcaaag agttgcttaa agtcctgagt gatgactgaa
    32161 ggaagagtga aaagcgtacc accaagtgcc aaggtcggtg cccaatacat gacagacaag
    32221 tcaaaagaat aaggtggctg tgccagcatt tgcggacgac tcggtgtcgc aaattcctta
    32281 tccgtaatca tccagtttgt aaagctgagg agattatcat gtgaaatctg cactccctta
    32341 ggcttaccag tcgtaccaga agtaaagata atgtagtaat tatcatctcc cttgactgga
    32401 tgcgtgattt catagttgtt cccttgggca aaggcttctt gaacctgagc tagatttatc
    32461 attggtgtag aaacctgctc caagggaaag gctgaaatgg caataatcaa gcttggctct
    32521 gctacttcta aaatagctga aactcgctcc aaggccgaat ggctatcaat tggaatgtag
    32581 gcatgacctg acttagtcag cgctacaaag gttgccaaca tttcatattc ttggccacca
    32641 aaaacaacca taggagactt ttcaggcaag cctagttggt caatgactgc agccaaacta
    32701 tccgaatcag cctttaaatc gccataagtg tgttcctgcc ccaaaacatt atagacagga
    32761 tagctaggct gtgtctgagc aaaatgctca atggtttcaa tcatatctgc tattggttta
    32821 tttgacacaa tagggattct ccttcaagtt aaaattcatt atagataaag cttccttgac
    32881 cctgaccaag atagctaaag aagtaaagca gccctagaaa gataagaaaa tacaaggctg
    32941 tccgaccaag aaagaggtac aattcttttc tctgtttcat caagaaaaac cattcatttc
    33001 tgtaattttt cgctaaaata agagtgattc ttactagctt atttttctac cattgtacca
    33061 ctttatatag tatcttttca attgtttacc gtatgtttcc aatagatttc agcttatttt
    33121 aaggattatg cggtttttct atgtatattt tcaaatagag tgatcctgct tcaaaactcc
    33181 atttcaggag acaatgaagt aaatcttccc ataataaaac acacaatatc aagttttttc
    33241 aacacctgat actatgcgct tttctgattt ttaaagactt tttaaccact ctctcattta
    33301 aaataatctc gtctgatata aattaaaata gcttctatca tcagacaaat ggctgatagc
    33361 caaaaactga tgctaatacc aaaactctca gtaatatagc tcattagcaa aacaaatact
    33421 gaaaatgcta atgtagaaat cacttcaaga acggaataga cattaactaa atgattttcc
    33481 tctactgttt cctgaagaaa tacactttca ggaacttctt ttagttgcga taacatacca
    33541 actaaagctg aaaataataa aaacatctgt gcgtttggaa aatatagaat agtcagtgtc
    33601 actattgcca tagctacaag aggaaaaaga atactttccc atttagcagc aaggaacttt
    33661 tcagatagcc taaaagcaat taagccacta attataatac caatagagta tgctgtatta
    33721 gaatatcccc agtaactttc cgttttattt aataactccg ttacaaaaac aagtataatg
    33781 gaagaaaccc aaatcgtatt tgaaaaaatt tccaataaat ttgctgatac aaaaagtctt
    33841 aatctagggt ttctagcaac taacttccaa cctttgagca aaatttcaag attagtttct
    33901 gactctaaca cctccacttc agcgttagga agaaataaca tcagaaagct agaaatgata
    33961 tacaagacta aaatgataaa cgtggtaggt aacagaccaa ttgttgcaaa caagagtcca
    34021 cctaatcccc aacctatcaa ttgaacagct tcaccagtca ttgataaggc tgaattagcc
    34081 ttacccaaat cggtcgcata gcgtggcaca atagcatagg aaacgggtgc tgcaaaacca
    34141 tctagtatgg aaattgcaac aacaaataga taggtcacca aaggcgctac ggattgcatt
    34201 acggtaaaca ttcctaccag tatcgccaat aatatagtct ttccaaattg agataaagat
    34261 aaaaccctat ttagcgctaa ccttttagta accaacggaa ctaaaagact cgcaacaaaa
    34321 gaggatattc ctattaagat aggaactagt gatgtggcaa ttactgattt tgaaataatg
    34381 tatatgttag caatgattgt tactctaaag aaaatatctg ctaaatttgc aaataattga
    34441 gagactaaca acttgacaaa tgaattagac atgtagcgct ccccctttag ttacaaactt
    34501 tggaattaaa tttgattttt aatattttag cataaaatag cagaaaacaa aagtgaggtt
    34561 ttttacatga aaaaacaaaa actattgcct ttattattcc taattttagg aggaattatg
    34621 attatcgttg ttggctagta ctaagaagtt agttagttag tagaataatt acatcttcaa
    34681 tcgaaaaaga gactggaaaa agtcttaaaa atcagaaaaa cgctcaaatc ctttggcgct
    34741 ctaagctttt ctaatgatag atttagatag tcaaaaaaat atctgaaaag ttcccaggat
    34801 cgtcgtctca gcatcaaaaa tacgtgaaat ttgcataatt aaaataaatt tcaggggagt
    34861 taatcgagta ggggataatc tctagcccct ctcacaccac cgtacgtgcc gttcggcata
    34921 cggcggttca actaactttt aacgcatgtc gttcaaggta ataatccaaa cacgaaacca
    34981 gtccacgttt ttccaggact ggttttgata tagcacgttt aagtaccgac ttctgagcta
    35041 ctaattgata atggtcgccc cagccagata ccttatctgc tatccattta ggaactccta
    35101 acttaagcaa tccccataat cgtctcgatt tcttcttcca ttgcttccag ataatcactc
    35161 gtaggcgagt acgcaagcgc tcatctatgc tggcgactat acttttcata tttcccaatg
    35221 agaaatagtt tatccatcct cgaatagaca aattcagttg ctcaatacgt cttgttaggt
    35281 ctatactcca tttcctctgt gttagtttct tcaatttaag cttaaatctc cgaacactat
    35341 cttgatgtgg acgacttttc caaccatctg atgatttcca gaacccaaaa cctagatatt
    35401 tcaactctct tggtctggta atcttagtct tggtcatgtt tactttcaaa cctagccgtt
    35461 tctcaataaa acgactgact gaatacatca cacgcttagc ggctgcctcg cttccgaccg
    35521 tatcacacaa tcatctgcgt agcgcacaaa tcgaagtccc ctcttttcta attccttgtc
    35581 caattcatta agcatgatat tggataagag aggagataaa tttcctccct gtggtgtacc
    35641 aactagtgtt ttataacgtt gaccattaat gataacacct gaatgaagat acttacgaat
    35701 caaggattcc gtatctccgt cttcgataat gttatgtact aaggacatca atctatcttg
    35761 aggaactgta tcgaaaaatt tctctaggtc tatatccact atccactcat agccgtcatt
    35821 taagtattct aagagcttca tgatggcttt ttcacatgac ctatttggtc tgaaaccata
    35881 actcgtatct gagaaatggg gttcacaaat ggggctcatg acttggacaa tggcctgttg
    35941 aatcattcta tccataactg ttggaattcc tagttgacgg atgcctccgt ctggtttagg
    36001 tatctcaact ttaagaactg gttgaggctt atattttctc tgttttatca gttccttagt
    36061 caagcgccag ttttgtctga gataattatc catctcttcg atagtcattc catcaatccc
    36121 agctgagcct ttattggatt ttacttgatt gtaggcttcc agcatatttt cgcgtgataa
    36181 tatcttatct agcagttttg acatgtgcgt attcctttct tgttttggtg cctgagggac
    36241 atcttatatt ggtgaacctt catctagtca ttacgtgtca aagtccagtt ctatcagacc
    36301 actcatttta cagacacaag tgaagtagga ttacttcgat ctgtcgtttt ctctttctag
    36361 cgacagacct caaacagtct ctccccagac tgtttgagta ttcacccctt cgctccactt
    36421 ccattacgga agtttcctca ctactatggg ctcggctgac ttctcatgat tcgttgttac
    36481 tacgctttca gcgctcatga aacctcacgg gataagtcgt acatctttcc tcgtttatac
    36541 aagatatgag gacgttaaaa ggttacagtg aaaataggaa atctgacgca gagtctttag
    36601 actctagaaa gatttatctt tttcacacaa accttagtcc gaattcaatt actctcgtga
    36661 tttacacata taggttacga ctaccttttt ggactttaga gtttgaagcc tccttatccg
    36721 ctatatacgc cttactatca cgttcctgtt cgtagagcca cgatttcgct atcccttcct
    36781 ctccctgcta cctcacgata gtcaggcttg ggaatcgcta ttgggttcac cggtagcagg
    36841 tgcccaccga ggacttacac ctcagatata cgacatgccc gtcgtactac aaaaaaacga
    36901 c
//
LOCUS       DAVKWZ010000015        36052 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000015 DAVKWZ010000000
VERSION     DAVKWZ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36052
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.015"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(1..712)
                     /locus_tag="VW854_000795"
                     /pseudo
     CDS             complement(1..712)
                     /locus_tag="VW854_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(924..1283)
                     /locus_tag="VW854_000796"
     CDS             complement(924..1283)
                     /locus_tag="VW854_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEV0262064.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSILAIILVFFFLLKKRSFYYPKFIKFFWRAGFL
                     LTLIMYIEMIVELFLMK"
     gene            1375..2331
                     /locus_tag="VW854_000797"
                     /pseudo
     CDS             1375..2331
                     /locus_tag="VW854_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            2315..2872
                     /locus_tag="VW854_000798"
     CDS             2315..2872
                     /locus_tag="VW854_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV0262065.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            2865..3119
                     /locus_tag="VW854_000799"
     CDS             2865..3119
                     /locus_tag="VW854_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262066.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTHHERSVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            3131..5185
                     /locus_tag="VW854_000800"
     CDS             3131..5185
                     /locus_tag="VW854_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEV0262067.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLILPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     ERGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            5364..6224
                     /locus_tag="VW854_000801"
     CDS             5364..6224
                     /locus_tag="VW854_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEV0262068.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLGFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            6435..7145
                     /gene="ccdA2"
                     /locus_tag="VW854_000802"
     CDS             6435..7145
                     /gene="ccdA2"
                     /locus_tag="VW854_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEV0262069.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            7165..7731
                     /locus_tag="VW854_000803"
     CDS             7165..7731
                     /locus_tag="VW854_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEV0262070.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            7742..8854
                     /gene="msrB"
                     /locus_tag="VW854_000804"
     CDS             7742..8854
                     /gene="msrB"
                     /locus_tag="VW854_000804"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV0262071.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEIMLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            9122..9859
                     /locus_tag="VW854_000805"
     CDS             9122..9859
                     /locus_tag="VW854_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0262072.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            9856..11547
                     /locus_tag="VW854_000806"
     CDS             9856..11547
                     /locus_tag="VW854_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV0262073.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            11663..12226
                     /locus_tag="VW854_000807"
     CDS             11663..12226
                     /locus_tag="VW854_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001006373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEV0262074.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFGLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALTSRPKLIGGARAHYPQDAIRKF"
     gene            12248..17860
                     /locus_tag="VW854_000808"
     CDS             12248..17860
                     /locus_tag="VW854_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV0262075.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGTMGLVVLPSAGAVDPVATLALASREGVVEMEGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEEAPKEEAPKTEESPKEEP
                     KSEVKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKPAEES
                     KVEQAGEPVAPRKDEQAPVEPENQPEAPEEEKAVEETPKQEESTPDTKAEETVEPKEE
                     TKTAKGTQEEGKEGQAPVQEVNPEYKVTTGTVEKSTESELDFTIEVVPDDTKYVDEEV
                     VERQGSKGVQITKTTYETVEGVETDKVLSTTTEVKTPAVPKVVKKGTKPVEGTTVETR
                     EEVIPFETKEQEDDTLKRGTRQVVQKGVEGKKQITETYKTIRGEKTNEAPIVKETVIE
                     QPQDEIIKKGTKGLEKPTLEWVKTDKDVLKKSATASYTLNKPDGVEIKSIKVALKDNT
                     GTVIKEVTVEENNLNATLDNLKYYQGYTLSTTMVYNRGEGEETEMLEDKEVQLDLKKV
                     EIKNIKETSLMSVDDAGVETDKSLLTEKPTDVAPLYLRVTTHDNKTTRLAVSSVEEVV
                     VDGKTLYKVVAKAPDLVQRREDDTLSEEYVHYFEKQLPKVDNVYYSFNELITDMQKNP
                     TGTFKLGADLNAANTPTPSKSYVTGEFKGKLSSVDGQHYTIHNTARPLFNNIVGGTVK
                     NINLNNVNIDMPWADQIASVANVIKGGSLIENVKVKGNVLGRNWVSGFIDKIDSGGRL
                     VNVAFIGNVTSVGDGDKFLTGIVGENWKGYVEKAYVEANIKGKRAKAAGISYWSQNSG
                     DNNAVGRDGAIKKSVVKGSIDVEKPIEVGGAVGSLNYLGYIEDTVAMMKVKNGEIFYG
                     SHDIDTDPYYTGERVNRNFIVDGVSEGKSSYTYSKQQKRIKSISQEEADKKIKELGIT
                     ADKFTIIDPIVNKLNNIKQKADTYKDTQDYKADRELAYRNIEKLQPFYNKEWIVDQGN
                     KVPSNSKLLTTEVLSVTGMKDGQFVTDLSEIDKIMIHYADGTKEEKSVSPKPTSNVEQ
                     VKEYGITDLGDVVYTPNMVVKDRAQLLSDVKAKLDTITLESPEVRAITGNVGALYLEE
                     SFAEVKGNLDKLVKALVENEDHQLNSDEAAIKAFLKKVEDNKAKIMMALTYLNRYYGI
                     EYGDLNIKNIMMFKPDFYGKIPSVIDRLIRIGSQEKNLKGDRTQNAYREIIAGDTGKG
                     DLRSFLDYNMRLFTNDTDLNDWFIHSAKNVYVVEPETTNPDFKNKRHRVFDGLNNNMH
                     ARMILPLLNLKKAHIFMISTYNTMAYSSFEKYGKNTEAEREAFKERINYVAKAQQTYL
                     DFWSRLALPNVRDRLLKSQNMVPTPVWDNQAYAGIKDANRRGYGTDGKVATPIRELFG
                     PTDRWHQINWNMGAMAKVYGKPYEDEQVFFMVTNMLEDFGISAFTHETTHINDRMAYL
                     GGHGHRPGTDLEAYAQGMLQTPDKSTSNGEYGALGINMAYHRQNDGNQWYNPDPDKLQ
                     SREQIDHYMKNYNDALMMLDHLEADAVLPQLNGNTSRWFKKMDRQYRSGNSGHQFDKI
                     RELTEDEKKVAINTIDDLITNNLITKHGAPGDGTYNPTDFRSAYVNMNMMTGIYGGNT
                     STGAPGAASFKHNTFRMWGYFGYENGFVGYASSKYQNAANKENNRMLGDDFIIKKVSN
                     GRFTNLEDWKKEYYKEVKAKGEKGFTAIEIDGKQITNYAELKVLFDKAVEADLAGGGT
                     AKTVELKSKVFKALLKNSDGFTGNLFKA"
     gene            17934..19655
                     /gene="pabB"
                     /locus_tag="VW854_000809"
     CDS             17934..19655
                     /gene="pabB"
                     /locus_tag="VW854_000809"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEV0262076.1"
                     /translation="MHRKTVIDFRILGERYTFTQPIKEIKTRNVAEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDRVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLSANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANQDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGKLLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQYLLETGQVEEKVLTLADLAQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            19656..20273
                     /locus_tag="VW854_000810"
     CDS             19656..20273
                     /locus_tag="VW854_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV0262077.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGAALALELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKTLMVGVSEELKTLDLTDIAGTCSYPTLLICGSKDKPNL
                     SSMKALHRLLTDSQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            20323..21322
                     /locus_tag="VW854_000811"
                     /pseudo
     CDS             20323..21322
                     /locus_tag="VW854_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
     gene            21449..22408
                     /locus_tag="VW854_000812"
     CDS             21449..22408
                     /locus_tag="VW854_000812"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEV0262078.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDREKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPIACTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            22505..23344
                     /locus_tag="VW854_000813"
     CDS             22505..23344
                     /locus_tag="VW854_000813"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEV0262079.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKFAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(23392..23562)
                     /locus_tag="VW854_000814"
     CDS             complement(23392..23562)
                     /locus_tag="VW854_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEV0262080.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            23683..24567
                     /gene="miaA"
                     /locus_tag="VW854_000815"
     CDS             23683..24567
                     /gene="miaA"
                     /locus_tag="VW854_000815"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEV0262081.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVSHHLIDVREITESYSAFDFVSEAKMTIEDIHSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVDQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQETLYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTLEEASESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            24560..25798
                     /gene="hflX"
                     /locus_tag="VW854_000816"
     CDS             24560..25798
                     /gene="hflX"
                     /locus_tag="VW854_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEV0262082.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKFQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTILSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            25791..26414
                     /locus_tag="VW854_000817"
     CDS             25791..26414
                     /locus_tag="VW854_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEV0262083.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGFGRIFSENEEKISDDLLFEIAQIFSHQLVFEESGKIYMKPVKDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            26429..27358
                     /locus_tag="VW854_000818"
     CDS             26429..27358
                     /locus_tag="VW854_000818"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEV0262084.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            27379..28134
                     /locus_tag="VW854_000819"
     CDS             27379..28134
                     /locus_tag="VW854_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV0262085.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEPDAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(28188..29096)
                     /locus_tag="VW854_000820"
     CDS             complement(28188..29096)
                     /locus_tag="VW854_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV0262086.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            29160..29429
                     /locus_tag="VW854_000821"
     CDS             29160..29429
                     /locus_tag="VW854_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEV0262087.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLKAEAVLRREHKVELDYIEKNGEKN"
     gene            29429..29809
                     /locus_tag="VW854_000822"
     CDS             29429..29809
                     /locus_tag="VW854_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV0262088.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            29898..30128
                     /locus_tag="VW854_000823"
     CDS             29898..30128
                     /locus_tag="VW854_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262089.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKES
                     QLDYKIEKILNILGIVISVVNWIVAIALIFRK"
     gene            complement(30164..30889)
                     /locus_tag="VW854_000824"
     CDS             complement(30164..30889)
                     /locus_tag="VW854_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEV0262090.1"
                     /translation="MRLDNLLAQEKVSRKAMKQALLRGEILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMNLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQTFQKGIVFLDGTIC
                     KPARLEILSASPSLSQASITISEGKFHQIKKMFLSIGVKVTNLKRIQFGDFTLNPDLA
                     EGNYHPLNQKELQIIKNYLEMSR"
     gene            31051..32892
                     /gene="typA"
                     /locus_tag="VW854_000825"
     CDS             31051..32892
                     /gene="typA"
                     /locus_tag="VW854_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEV0262091.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            32912..33166
                     /locus_tag="VW854_000826"
     CDS             32912..33166
                     /locus_tag="VW854_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEV0262092.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     gene            complement(33429..34595)
                     /locus_tag="VW854_000827"
     CDS             complement(33429..34595)
                     /locus_tag="VW854_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV0262093.1"
                     /translation="MKITEYTKKDGSTVYRSSVYLGIDTVTGKKVKTTISGRTKRELK
                     AKALQAQIDFEKDGSTVYKTVEIKTYAELVENWLETYCHTVKKATLTIIKSRLNNYVL
                     PAFGEYKLDKLTPPVIQKQVNQWAKEYNQLGKGYQEYPQLNSLNKRILKYAVSLQVIP
                     FNPARDIIVPRRKEKEGQKLKYLDDDNLKKFLTYLEQLPNTYKNFYDTVLYKTLLATG
                     LRIRECLALKWSDIDLQNGTLDVNKTLNCEKEVTTPKTKSSIRVIDLDNKTVLMLRLY
                     KNRQAQVGREIGLTYEKVFSNSFDEYRDARALRSRLEKHLKLSDCPRLTFHAFRHTHA
                     SILLNAGLPYKEIQTRLGHSQISITMNTYSHLSKDNKKNATSFYEKAIEKLKSS"
     gene            complement(35486..35716)
                     /locus_tag="VW854_000828"
     CDS             complement(35486..35716)
                     /locus_tag="VW854_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262094.1"
                     /translation="MDYAQDYLDDILVRMAYHSSGIEGNTISLPETVSIILEKSIIVM
                     FNIANTSQRINVNAYTYFQNKNKKVQKKRQKS"
     gene            complement(35736..35948)
                     /locus_tag="VW854_000829"
     CDS             complement(35736..35948)
                     /locus_tag="VW854_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HEV0262095.1"
                     /translation="MKFHYVNKWIYIILAIVLGWIGGHHFYAGYNRKGFLYLLFSFTF
                     IPMLLALFQVISALLKTPDSNGKILV"
BASE COUNT        10890 a         6540 c         7994 g        10628 t
ORIGIN      
        1 tgcgttgagc gaaaatctgt ctaccttggg gagataaaag cgcctgacat tctttagctt
       61 tcaagttttg atagcgttcg ttcatataca gtcccctttt gaggggctga ttcaggttcg
      121 tcggcgtagt aaaccttgat ttcctgttga aagtctgtct gtgttttctg gtgcttgata
      181 tggtgaaaac gatagcacca gccatcagga tgtatgtagc tatcctcctt gtcattatag
      241 tgccaattcg ctaagttttt agctgactct ttataccctc tcttttgttt cttatcaaac
      301 atggcatatt taatcagaag gggagtgatt tcttcttgaa aatagacttg tatctgttct
      361 tgaagtttgg cggagaattt ctctgtcgtt ttcttccaca caaaactata cttgttggca
      421 ttggcttcaa tcttagttcc gtcaataaac aaacaatcta aggtcactaa ctcttccatt
      481 tttcaatttt tcgaccagag aaaatccctt gtgaataggc aaatagaaga gtagatacaa
      541 gcattttagg gtgataagac gggcgaccaa aggcatgata gaaggcgtag aagtgatgtt
      601 cctccaaggt attcaccact ttttcaatag taaagacgag atggtcttgt ggtaagaagg
      661 aactgatttc tagtggtaaa gttgtttgat ttgtgttata gtgaatatgc atgggatagc
      721 ctttctaaat ggtttattgg gcaattctat tttaccaaga ctatcgtaac catgcaaaaa
      781 aagcaacggc aaatccgttg ctttttttgg agataagaga ctattgtccc agactctttt
      841 ctttgtataa aattgtatag tgttattgtc tatactattt tgcagagaaa gcaaaaatcc
      901 ctagaaaatg cttagggatt cgactatttc attaagaaca attcaacaat catttctata
      961 tacatgataa gggttaataa gaatcctgca cgccagaaga atttgatgaa tttaggatag
     1021 taaaagctgc gttttttcaa aaggaagaaa aagacgagaa taatggctag gattgagagg
     1081 gctagcccca gtctagggag gaaattatgg gtaaaggttt tagctgtaat caggtaatat
     1141 tcaaatacca agaccggaaa agccaaatcc gcaaagtttc gtcctagttt ttttaatcta
     1201 aaaagtttgg ttaggataat gcagacgact aaggtcagta tcaataataa aatagatgct
     1261 aatttcatta aaatcatacc catattgtat cataaaaaaa agctaatgga aacgagaaac
     1321 agaggaattt ataggaaagt caggcttttg agattgtggg tgatttttgt tataatgaaa
     1381 gttataaaat cttataatac cttgaatgat tattatcgaa aactctttgg agaaaagact
     1441 tttaaagtcc ctattgatgc ggaatttgac tgtcccaatc gtgatggaac tgtggctcat
     1501 ggaggctgta ctttttgtac ggtttcaggt tctggagatg ctattgtagc cccagatgcg
     1561 cctatccgtg agcaatttta taaggaaatt gactttatgc accgtaagtg gccggatgtt
     1621 cagaagtatc tggtttattt tcaaaatttt accaacaccc atgaaaaggt ggaagttatt
     1681 cgagagcgtt atgaacaggc tatcaatgag ccaggtgtgg taggaatcaa tattggaatg
     1741 cgcccagact gtttaccaga cgaaaccatc gaatatttgg ctgagttatc ggagtgcatg
     1801 catgtgacgg ttgaattggg cttgtagact acttatgaag caacttctga cctgattaac
     1861 cgtgcgtatt cttatgaatt gtaggtggaa acggtcaagc gtttgagaaa gtatcccaag
     1921 attgagattg tttcccattt gattaatggt ttgcctggtg aaacccatga gatgatggtt
     1981 gaaaatgtcc gccgttgtgt cacgaataac gatattcaag ggattaaact gcatttgctc
     2041 catcttatga caaatactcg tatgcaacga gattaccatg agggacgttt acaattgatg
     2101 agtcaggacg aatatgtcag gggcatctgt gaccaactgg aaatcattcc caaacatatc
     2161 gtcatccatc gaatcacagg agatgcacct agggatatgc tgattgggct tatgtggagc
     2221 ctcaataaat gggaagctct caacagcatt gagatggaga tgcgacgtcg tggaagtgtt
     2281 caaggatgca aggctgtaaa acaggagttt gaaaatgaaa agaccacttg agatggcaca
     2341 tgattttttg gctgaggtcg tgacaaaaga ggatgtcgta gtggatgcga ctatgggaaa
     2401 tggtcatgac acgctttttt tagccaagct agccaagcaa gtctatgcct ttgatattca
     2461 gaagcaagcc ttggaaaaga cccaagagcg tttgcatcag gctggcttga caaatgccca
     2521 gttaatcttg caaggccatg agacactgga ccagtttgtg acaaaagcta aggcagggat
     2581 ttttaatttg ggctatttgc cgtcagctga taagtctgtc atcacccgac cgcagacaac
     2641 gattgaggca ttagaaaagc tatgtggctt acttgtcaaa ggtggacgaa ttgctattat
     2701 gatttactat ggtcatgaag gaggcgacct cgagagagat gctgtcttgg attttgtaat
     2761 ccagttgaac caacaagagt acacagctgc catttaccga actttaaacc aagtcaacaa
     2821 cccgccgttt ttagtgatga ttgaaaaatt agagagatac agacatggat aaacaatacc
     2881 tacacgaaaa gctggatgcc atgcgccaga attttgttga atcaacccac cacgaacgat
     2941 cggtgggcgt gctagaccaa gcgcatatga gcaaaaaaat gcttaaaatc aagaaaaaat
     3001 tagttgctct tgaaatggaa cggtgccaga gaaaaattga gcacaaagac tgttccaaga
     3061 ttgaccaaaa aatcaaagag cagaaggaga tatttgaatc ctgttgtaaa aaagattaag
     3121 gaggacgtgc gtggaattac tgatttacct catcctattt ttactggttt tgattgtctc
     3181 aagtacaacc aataagctcc tgcccttttt gcctctccct ttggtgcaaa ttcttttggg
     3241 aattgtgatt ggtctctttt tacccaatac cgactttcac ctcaatacgg agttgttttt
     3301 ggccctggtt atcggaccct tgcttttccg agaggctgaa gaagcagatg ttacggctat
     3361 tttaaaacac tggcgaatca ttgtttatct catatttcca gtgattttta tctcgaccct
     3421 gagtttgggt ggcttggccc atcttctttg gttcagcctt cccttggcag cttgcttggc
     3481 tgttggggca gcccttggtc ctacggactt ggtggccttt gcctctcttt cggagcgttt
     3541 tagctttcct aagcgcgtgt ccaatattct taagggcgaa ggactcttga atgatgcttc
     3601 tggtttggtg gcttttcagg tagctttgac agcttggaca actggagctt tttctctggg
     3661 gcaagctagc agttcgctca tcttttcaat cctaggcggt tttttaattg gatttttaac
     3721 agccatgacc aaccgcttcc tccatacctt cttgctaagt gtgcgcgcaa cggatattgc
     3781 cagtgaactt ttattagaat taagtttgcc tctagtgacc ttctttctgg cagaagaagt
     3841 tcatgtttca gggattattg ccgtcgtagt tgctggaatt ttaaaggcaa gtcgcttcaa
     3901 gaaaatcacg ctcctcgaag cccaagtgga tacggtgacc gagacggtct ggcatacagt
     3961 gacctttatg ctcaacggtt ctgtctttgt gattttaggg atggagttgg aaatgatagc
     4021 agaacctatc ttgaccaatc caatctataa tcctctactt ttattgctat ctctcatcgc
     4081 ccttaccttt gtcctctttg tcattcgttt tattatgatc tatggctatt atgcctatag
     4141 aacccgacgc ctcaagaaaa agctaaataa gtatatgaag gacatgtttc tcttgacctt
     4201 ttcaggtgtt aagggaacgg tgtcgattgc tacgattctc ttgataccaa gtaatctaga
     4261 acaagagtat cctcttttgc ttttccttgt tgcaggtgtg acgcttgtca gctttttaac
     4321 aggtctcttg atcttgcctc atctttctga tgaagaggaa gaaagcaagg attatctcat
     4381 gcatatcgcc attttgaatg aagtaacgct agagttggaa aaagaattgg aagacaccag
     4441 aaataaactt cccctctatg cggctattga caattatcat ggacgtattg aaaatctcat
     4501 tttaagccaa gaaaaccagg atgatcaaga agactgggct gctttgaaac tcttgattct
     4561 tagtattgaa agtgatggtt tggaacaggc ctatgaagag gggaacatta gcaatcgtgc
     4621 ttaccgagtt taccaacgtt atctgaaaaa tatagaacga ggaatcaatc gtaaacttgc
     4681 ctcaagactg acctattatt ttcttgtttc cttgaggatt ttacgttttc tccttcatga
     4741 agtttttact cttggaaaga ccttccgtag ctggaaggac aaggagcaaa gccgtctccg
     4801 tgctcttgat tatgaccaaa ttgcagagct ctatcttgcc aatacagaga tgattattga
     4861 aagtttggaa aacctgaagg gagtctacag acgctctttg attagtttta tgcaggagtc
     4921 tcgtcttcga gaaacggcta ttatcagcag tggtgccttt gtcgaacggg ttatcaatcg
     4981 tgtcaaaccc aacaatatcg atgaaatgct gagaggctat tatctggagc gcaagttgat
     5041 tttcgaatac gaagaaaaac gattaattac aactaagtat gccaagaaat tacgacaaaa
     5101 tgtaaataac ttagagaact attccttgaa ggaagctgcc aataccctgc cgtatgatat
     5161 ggtggaattg gtaagaagaa attagttaat actctttgaa aatctcttca aaccacgtca
     5221 gcgtcgcctt accgtatgta tgtgactgac ttcgtcagtc ttatctacaa cctcaaaaca
     5281 gtgttttgag cagcctgtgg ctagcttcct agtttgctct ttgattttca ttgagtataa
     5341 gattataagt gaaggagtgt gacatgaaaa agtggtggaa agagctgata gacaagcctt
     5401 tattaaaagc ttttttgcat tattatcaag catcagatag tgagttgacc agtgtcgcag
     5461 tagcctacta ttggttgatt tcgattttcc ccctgctttt ggtggtggtc aatatcctcc
     5521 cctattttca gattcctgtg ggagaatttc tgggctttat gaaagacgtc ctgcccccaa
     5581 gcctctatga aggtgtggaa aaaatagcca gagaagtctt gacgcaacct tcaacaggtt
     5641 tattgagttt ttctgtttta tcggcgttat ggagtttttc aaaatctatg aatttcttgc
     5701 aaaaagcttt taacaaggct tatggcgtcg aaaagagtcg tggacttatt tcccatcaga
     5761 tgctaagtct cctagtcagt tttggcttgc agctcctctt tgcgtttgcc ttgtttttga
     5821 tcttatttgg tcagatgatt ttggctttac ttgctcatta ttggacaaaa gatggcatta
     5881 tctatcaggc tttacaaggg ttggcaggtc ctctgattta cgccctcctc tttgctatct
     5941 tggtcatgct ttattatttt cttcccaatc tatctaacag gaaaattagc tataccctac
     6001 caggcagtgc ctttgttctc ttagtgattt taggtttact aactctgttc tctagttatc
     6061 tcaattacta tgtccatcat ttggtggatg tccgaatttt aggttccgtt cttcttgttg
     6121 ttatgatgtt ttggtttatc ttgattgcta aaattgttat cctaggcgcg gttatcaatg
     6181 ccagtatgca gagtttgaaa gatccggtct ttaaaaaaga ctaactattt atccattata
     6241 aaagcgcata gtatcaagac tttatagaac cttgatacta tgcgcttttg tgctttgaca
     6301 gttttttgta tagtttttgt ccaagatttc taaatcatct gattaaattc ctattttccc
     6361 ctatcattgt ccctgttttt tcgaaaatgg aaggagtata ataaatctat caataaaatg
     6421 ataggaggaa ctttatgggt catattttct tttttctaag tgtctttttg gcagggattc
     6481 tatccttctt ttctccttgt atcttacctt tgttaccggt ctatacagga gtgttactag
     6541 atgataagga tggtgctcag gcttctagcg gcaaattttc aatctcagtt actagtttat
     6601 tacgaactct ggcctttata gcaggaattt cctttatatt tattttgttg ggctatggag
     6661 ctggtttttt aggcgatttg ctttatgctt cttggttcca atatcttact ggggcaatta
     6721 ttatccttct tggtttgcac caaatggaga ttctacactt taaggggctt tataaggaaa
     6781 agagactaca actgcaagga caggggcaaa atggtaaggg ctatagtcag gcatttttat
     6841 tgggcttgac ctttagtttt gcttggacgc cttgcgtggg gccggttctg gggtctgttt
     6901 tggccttggc ggcttcaggt ggttcaggag cttggcaggg agctggtctc atgttggtgt
     6961 atacgctggg cttggcgcta ccattcttgc ttctagctct gacctctagt tatgttttga
     7021 aacatttccg aaaacttcat ccctatctcg gaatcctcaa aaaagtgggt ggttttctca
     7081 ttattgtgat gggactcttg gttctgtttg gaaatgcttc aattttaagt caattatttg
     7141 aataaaatgg aaaggaatat caatatgaaa aaatggcaaa catgtgttct tggagcaggt
     7201 tcgctccttt gtttgacggc ttgttcaggc aagtccgtga ctagtgaaca ccaaacgaaa
     7261 gatgaaatga agacggagca gacagctagt aaaacaagcg cagctaaagg gaaagaggtg
     7321 gctgattttg aattgatggg agtagatggc aagacctacc gtttatctga ttacaagggc
     7381 aagaaagtct atctcaaatt ctgggcttct tggtgttcca tctgtctggc tagtcttcca
     7441 gatacggatg agattgctaa agaagctggt gatgactatg tggtcttgac agtagtgtca
     7501 ccaggacata agggagagca atctgaagcg gactttaaga attggtataa gggattggat
     7561 tataaaaatc tcccagtcct agttgaccca tcaggcaaac ttttggaaac ttatggtgtc
     7621 cgttcttacc caacccaagc ctttatagac aaagaaggca agctggtcaa aacacatcca
     7681 ggattcatgg aaaaagatgc aattttgcaa actttgaagg aattagccta ggaggtgtct
     7741 tatgaatgat aagttaaaaa tcttcttgtt gctaggagta ttttttctag ccataaccgg
     7801 tttctatgtt ctattgatac gaaatgcagg gcagacagat gcctcgcaaa ttgaaaaggc
     7861 ggcagttagc caaggaggaa aagcagtgaa aaaaacagaa attagtaaag acgcagactt
     7921 gcacgaaatt tatctagctg gaggttgttt ctggggagtg gaggaatatt tctcacgcgt
     7981 tcccggggtg acggatgccg tttcaggcta tgcaaatggt agaggagaaa caaccaagta
     8041 cgaattgatt aaccaaacag gtcatgcaga aaccgtccat gtcacctatg atgccaagca
     8101 aatttctctc aaggaaatca tgcttcacta tttccgcatt atcaatccaa ccagcaaaaa
     8161 taaacaagga aatgatgtgg ggacccagta ccgtactggt gtttattaca cagatgacaa
     8221 ggatttggaa gtgattaacc aagtctttga tgaggtggct aagaaatacg atcaacctct
     8281 agcagttgaa aaggaaaact tgaagaattt tgtggtggct gaggattacc atcaagacta
     8341 tctcaagaaa aatccaaatg gctactgcca tatcaatgtt aatcaggcgg cctatcctgt
     8401 cattgatgcc agcaaatatc caaaaccaag tgatgaggaa ttgaaaaaga ccctgtcacc
     8461 tgaggagtat gcagttaccc aggaaaatca aacagaacga gctttctcaa accgttactg
     8521 ggataaattt gaatccggta tctatgtgga tatagcaact ggggaacctc tcttttcatc
     8581 aaaagacaaa tttgagtctg gttgtggctg gcctagtttt acccaaccca tcagtccaga
     8641 tgttgtcacc tacaaggaag ataagtccta caatatgacg cgtatggaag tgcggagccg
     8701 agtaggagat tctcaccttg ggcatgtctt tacggatggt ccacaggaca agggcggctt
     8761 acgttactgt atcaatagcc tctctatccg ctttattccc aaagaccaaa tggaagaaaa
     8821 aggctacgct tatttactag attatgttga ttaatactct tcgaaaatct cttcaaacca
     8881 cgtcagcgtc gccttaccgt atgtagggtt actgacttcg tcagttttat ctacaacctc
     8941 aaagctatac tttgagctga cttcgtcagt tttatttaca acctcaaagc tatactttga
     9001 gaaacctgtg gctatcttcc tagtttactt tttgattttt attgagtata agaggtcttt
     9061 cctaagcagt tagaggagaa ttttgctata ctgatactag taagtgacaa aggagagaag
     9121 catgacctac acaatcttaa tcgtagaaga tgaatatctg gtaagacaag gtttgactaa
     9181 actggtcaat gtagcagcct acgatatgga aatcatcggt caggctgaaa atggaaggca
     9241 ggcttgggaa ttgatccaaa agcaggtgcc agatatcatt ttaaccgata tcaacatgcc
     9301 tcatctaaat ggcatccagt tggccagtct ggtacgagaa acctatcctc aggttcattt
     9361 ggtcttttta acaggttacg atgattttga ttatgccttg tctgctgtca aactaggtgt
     9421 ggacgactac ctgctcaaac ccttttctcg tcaggatatt gaggaaatgt tggggaaaat
     9481 caaacaaaaa ctagacaagg aagagaaaga agagcagtta caagatttat tgactaatag
     9541 gtttgaagga aacatggccc agaaaatcca gtctcatctg gctgatagcc aatttagttt
     9601 aaagtcttta gccagtgact taggttttag tccgacctat ctgagttcct tgattaagaa
     9661 agagttgggc ttgccttttc aggattatct ggtgagagaa cgtgttaaac aagccaagct
     9721 cttgctttta actacagatc tgaagattta tgagatcgca gagaaggttg gttttgaaga
     9781 tatgaactat tttacccaac gttttaagca gattgcaggt gtgacacctc gtcagtttaa
     9841 gaagggagaa gaccgatgaa gcgttcttct cttttagtta gaatggttat ttccatcttt
     9901 ctggtctttc tcattctcct agctctggtt ggaactttct actatcaatc aagttcttca
     9961 gccattgagg ccaccattga gggcaacagc caaacgacca tcagccagac tagccacttt
    10021 attcagtctt atatcaaaaa actagaaacc acctcgactg gtttgaccca gcagacggat
    10081 gttctggcct atgctgagaa tcccagtcaa gacaaggtcg agggaatccg agatttgttt
    10141 ttgaccatct tgaagtcaga taaggacttg aaaactgttg tgctggtgac caaatctggt
    10201 caggtcattt ctacagatga cagtgtgcag atgaaaactt cctctgatat gatggctgag
    10261 gattggtacc aaaaggctat tcatcaggga gctatgcctg ttttgactcc agctcgtaaa
    10321 tcagatagtc agtgggtcat ttctgtcact caagaacttg ttgatgcaaa gggagccaat
    10381 cttggtgtgc ttcgtttgga tatttcttat gaaactctgg aagcctatct caatcaactc
    10441 cagttggggc agcagggctt tgccttcatt atcaatgaaa accatgaatt tgtctaccat
    10501 cctcaacaca cagtttatag ttcgtctagc aaaatggagg ctatgaaacc ctacatcgag
    10561 acaggtcagg gttatactcc tggtcacaaa tcctacgtca gtcaagagaa gattgcagga
    10621 actgattgga cggtacttgg cgtgtcatca ttggaaaagt tagaccaggt tcggagtcag
    10681 ctcttgtgga ccttgcttgg ggccagtgtc acatctcttc ttgtctgtct ctgcttagtg
    10741 tggttcagtc ttaaacgctg gattgctcct ttgaaggatt tgagagaaac catgttggaa
    10801 attgcttctg gtgctcaaaa tcttcgtgcc aaggaagttg gtgcctatga actgagagaa
    10861 gtaactcgcc aatttaatgc catgttggat cagattgatc agttgatggt agctattcgt
    10921 agccaggaag aaacgacccg tcagtaccaa cttcaagccc tttcgagcca gattaatcca
    10981 catttcctct ataacacttt ggacaccatc atctggatgg ctgaatttca tgatagtcag
    11041 cgagtggtgc aggtgaccaa gtccttggca acctatttcc gcttggcgct caatcaaggc
    11101 aaggacttga tttgtctctc tgacgaaatc aatcatgtcc gccagtatct ctttatccag
    11161 aaacaacgct atggagataa gctggaatac gaaattaatg aaaatgttgc ctttgataat
    11221 ttagtcttac ccaagctggt cctacaaccc cttgtagaaa atgctcttta ccatggcatt
    11281 aaggaaaagg aaggtcaggg ccatattaaa ctttctgtcc agaaacagga ttcgggattg
    11341 gtcatccgta ttgaggatga tggcgttggc ttccaagatg ctggtgatag tagtcaaagt
    11401 caactcaaac gtgggggagt tggtcttcaa aatgtcgatc aacggctcaa acttcatttt
    11461 ggagccaatt accagatgaa gattgattct agaccccaaa aaggaacgaa agttgaaata
    11521 tatataaata gaatagaaac tagctaactc ccagtcaatt ctgggagttt tatgcgtaat
    11581 tgggcaaact ttctaggttg tctatcttgc taaaaacaag aaaaaacggt atgatagaag
    11641 agtgacaaca gaggtaacag agatgaacga gacacagatt caaagagaaa cacgtcaggt
    11701 agtggaagat gttttagaaa agaccaattt gaaacagggg gccctttttg ttttaggtct
    11761 atcttctagt gaggtgctag gtggtcagat tgggaaagaa tccagtcaag agattggtga
    11821 gctgatagtg gagactatct tgggtattct aggtagtaga ggtatccatt tagcagttca
    11881 aggttgtgag catgtcaatc gggctctcgt tgttgaacgt caggtggcag agcagtttgg
    11941 tctggaaatt gtcagtgtcc atcctactct tcatgcagga ggttcggggc agttggcagc
    12001 ctttaagttt atgcaggatc cagttgaggt tgaatttatc aaggctcatg ctggattgga
    12061 tatcggagac actgcaattg gcatgcatgt caagcatgtt caggttccga ttcgccctat
    12121 tttgagagag attggtcatg cccatgtaac ggcactgact agtcgtccaa aattaatcgg
    12181 aggtgcgcgt gcgcactatc cgcaagatgc tattaggaag ttttgagaat gaataaagga
    12241 gataaaagtg tttaaaaaag accgtttttc aattcgtaag attaagggag ttgtaggctc
    12301 tgtatttctt ggaagccttt tgatggctcc ttctgtagtg gacgcagcca cctatcacta
    12361 tgtaaataaa gagattattt cacaagaagc taaagattta attcagacag gaaagcctga
    12421 caggaatgaa gttgtatatg gtttggtgta tcaaaaagat cagttgcctc aaacagggac
    12481 agaagcatct gttttgacag cttttggttt gctgactgtt gggagcttgc ttttaatcta
    12541 caagagaaag aaaattgcta gcgtctttct agttggaact atgggattgg tagttcttcc
    12601 tagtgcaggg gctgtagacc cagttgcgac cctagcactg gctagtcgag agggtgttgt
    12661 tgaaatggag ggctatcgct atgttggtta tctatcaggt gacatcctca aaacgcttgg
    12721 cttggacact gttttagaag aaacctcagc aaaacctgga gaggtgactg tagtcgaagt
    12781 tgagactcct caatcaacaa caaatcagga gcaagctagg acagaaaacc aagtagtaga
    12841 gacagaggaa gctccaaaag aagaagcacc taaaacagaa gaaagtccaa aggaagaacc
    12901 aaaatcggag gtaaaaccta ctgacgacac ccttcctaaa gtagaagagg ggaaagaaga
    12961 ttcagcagaa ccagctccag ttgaagaagt aggtggagaa gttgagtcaa aaccagagga
    13021 aaaagtagca gttaagccag aaagtcaacc atcagacaaa ccagctgagg aatcaaaagt
    13081 tgaacaagca ggtgaaccag tcgcgccaag aaaagacgaa caggcaccag tcgagccaga
    13141 aaatcaacca gaagctcctg aagaagagaa ggctgtagag gaaacaccga aacaagaaga
    13201 gtcaactcca gataccaagg ctgaagaaac tgtagaacca aaagaggaga ctaaaacagc
    13261 taaaggtacg caagaagaag gcaaagaagg tcaagcacca gtacaagaag taaacccaga
    13321 atataaagta acaacaggta cagttgagaa gtctaccgaa agtgaattag atttcacaat
    13381 agaagtagtt ccggatgaca caaaatatgt agatgaagaa gttgttgaac gtcaagggtc
    13441 taaaggagtt caaattacga aaacaactta cgaaacagtg gaaggtgtgg aaacagacaa
    13501 agtcctttct acaactacag aagtaaaaac tcctgctgtt cctaaagtcg ttaaaaaagg
    13561 aacaaaacca gttgaaggaa caacagttga aacaagagaa gaagtcattc cttttgaaac
    13621 aaaagaacaa gaagatgaca ctttaaaacg cggaacaaga caagtagttc aaaaaggtgt
    13681 agaaggtaag aaacaaatta ctgaaactta taaaacaatt cgtggagaaa aaacaaatga
    13741 agctccaata gttaaggaaa cagtaattga acaacctcaa gatgagatta tcaaaaaagg
    13801 cacaaaaggt ttagaaaaac caaccttaga atgggttaaa acagataaag atgtcttgaa
    13861 gaagagtgcg acagccagtt atacattgaa taaaccagat ggggtagaaa tcaaatccat
    13921 caaagtagct ctaaaagata atactggaac agttattaaa gaagtaacgg tagaagaaaa
    13981 taacttaaac gctactttag acaacttgaa atattatcaa ggttatacac tatccacaac
    14041 catggtctat aatcgaggcg aaggcgaaga aactgaaatg ttagaagata aagaagttca
    14101 gttagacctt aaaaaagttg aaatcaaaaa catcaaagaa actagcttga tgagtgtgga
    14161 tgatgctggt gttgaaacag ataagagctt acttactgaa aaaccgacag acgttgctcc
    14221 actttaccta cgagtgacaa ctcatgataa caaaacaaca agactggcag taagttcagt
    14281 tgaagaagtt gttgttgatg gaaaaacatt gtacaaagtg gtagctaaag ctcctgattt
    14341 agttcaacgt agagaagatg atacactaag cgaagagtat gtacattact ttgaaaaaca
    14401 attaccaaaa gtagataatg tgtactatag ctttaacgag ttaattacag atatgcaaaa
    14461 aaatcctact ggtacattca aactaggtgc agatcttaat gcagcgaata ctccaactcc
    14521 aagtaaatca tatgtaacag gtgaatttaa aggtaaatta tcaagtgttg atggtcaaca
    14581 ttatacgatt cacaatactg cgcgcccatt atttaacaat attgttggtg gtaccgttaa
    14641 aaatattaat cttaacaatg taaatatcga tatgccttgg gctgatcaaa ttgcatctgt
    14701 agcaaatgtg atcaaaggtg gtagccttat tgagaatgtt aaagtcaaag gtaatgtgct
    14761 cggcagaaac tgggtatctg gtttcattga taaaattgac agtggtggta gattagttaa
    14821 tgttgcattt attggtaatg taacttctgt tggagatggg gataagttct taacaggtat
    14881 tgttggagaa aactggaaag gatatgtcga aaaagcatac gttgaagcta atattaaagg
    14941 taagcgagct aaagctgctg gtatatctta ttggtcgcaa aatagcggag ataataatgc
    15001 tgtcggtaga gatggagcaa ttaagaaatc tgtcgttaaa ggttcaatag atgtagaaaa
    15061 accaattgaa gttggtggcg ctgttggaag tttaaattat cttggttata tagaagacac
    15121 tgttgctatg atgaaagtta agaacggtga aattttctac ggttcgcatg atatcgatac
    15181 cgatccatat tacacaggag aacgagtaaa tagaaatttc attgttgacg gtgtaagtga
    15241 aggtaaatct tcgtatacat actctaaaca acaaaagcgt attaaatcaa tatctcaaga
    15301 ggaagcagat aagaaaatta aagaattggg tatcacagca gataaattta caattatcga
    15361 tccaattgta aataaactta acaacatcaa acaaaaagct gatacttata aagatactca
    15421 agattacaaa gcagaccgtg aacttgctta tcgtaatatc gaaaaacttc aaccattcta
    15481 taataaagag tggattgtag accaaggtaa taaagttcca tcaaattcta aattattaac
    15541 tacagaagta ttatctgtta caggtatgaa agatggtcaa tttgtgactg acttatcaga
    15601 aattgataaa attatgattc actatgcgga tggtacaaaa gaagaaaaat ctgtatcacc
    15661 taagcctaca tcaaacgtag aacaagtaaa agaatatggt attacagatt taggagatgt
    15721 cgtttacaca ccaaatatgg tggttaaaga ccgtgctcaa ttactaagtg atgttaaagc
    15781 gaaattagat acgattactc ttgaatctcc tgaagttcgt gcgataacag gtaatgttgg
    15841 agcattatac ttagaagaaa gttttgcaga agtaaaagga aatcttgata aattagtaaa
    15901 agctcttgtt gaaaatgaag atcatcagtt aaatagtgat gaggcagcca tcaaagcatt
    15961 tcttaagaaa gtagaagata ataaagctaa aatcatgatg gcacttactt acctcaatcg
    16021 ttattatggt attgaatatg gtgacttaaa cattaagaat ataatgatgt ttaaaccaga
    16081 tttctatggc aagataccaa gtgttattga tagattaatt cgaattggtt cgcaagaaaa
    16141 gaacttgaag ggtgatagaa cccaaaatgc atatcgtgaa attattgcag gagatactgg
    16201 taaaggtgat ttacgtagtt tcttagatta taatatgcgt ctcttcacaa atgatacaga
    16261 tcttaatgac tggtttatcc attcagcgaa aaatgtttat gttgtagaac cagaaacaac
    16321 aaatccagat ttcaaaaata aacgtcatcg tgtttttgat ggtctgaata ataatatgca
    16381 tgcacgtatg attttaccat tattgaactt gaaaaaagca catatcttta tgatttcaac
    16441 ctataatact atggcttata gttcatttga aaaatatggt aaaaatacgg aagctgaacg
    16501 tgaagcgttc aaggaacgta tcaattatgt agctaaagct caacaaacat acttagactt
    16561 ctggtcacgt cttgcacttc caaatgttcg tgatagactt cttaagagtc aaaatatggt
    16621 tcctacacct gtatgggata atcaagctta tgcgggtatt aaggatgcta atcgtcgtgg
    16681 atatggtaca gatggtaagg tagcgacacc aattcgtgag ttatttggtc caacagacag
    16741 atggcatcaa attaattgga atatgggagc tatggctaaa gtttatggaa aaccatatga
    16801 agatgaacaa gttttcttca tggtaacaaa tatgttagag gactttggta tttcagcctt
    16861 tacacatgaa acaactcaca taaatgaccg tatggcatat cttggtggtc atggacatcg
    16921 tccaggtaca gaccttgaag catatgctca aggtatgtta caaacacctg ataaatcaac
    16981 atcaaatggt gagtatggag cattaggtat taatatggct tatcatcgtc aaaatgacgg
    17041 aaatcagtgg tacaacccag atcctgataa attacaaagt cgtgaacaaa ttgatcacta
    17101 tatgaagaac tacaatgatg ctcttatgat gttagatcat cttgaggctg atgctgtatt
    17161 accgcagtta aatggaaata cgagtcgctg gttcaagaaa atggatagac aataccgtag
    17221 tggaaattca ggtcatcagt tcgataaaat tcgtgagttg acagaggatg agaaaaaagt
    17281 agcaatcaat acaattgatg atttaattac taataaccta ataacgaaac atggtgctcc
    17341 tggtgatggt acttataatc caacggactt taggtctgct tatgttaata tgaacatgat
    17401 gactggtatt tatggtggaa atacaagtac aggtgcaccg ggtgcagcat catttaaaca
    17461 taatacattc cgtatgtggg gatactttgg atatgagaat ggatttgttg gatatgcttc
    17521 tagcaaatat caaaatgcag ccaataaaga aaataatagg atgttaggtg atgacttcat
    17581 tattaagaaa gtttcaaatg gaagattcac taaccttgaa gattggaaga aagagtacta
    17641 taaagaagtg aaggcaaaag gtgaaaaagg atttactgca attgagattg atggtaaaca
    17701 aattaccaat tatgctgaac taaaagtctt atttgataag gcggttgagg ctgatctagc
    17761 aggtggagga acagctaaaa ctgtagaact taaatcgaaa gtctttaaag cacttcttaa
    17821 gaattcagac ggattcacag gaaatctttt taaagcatag attgtattaa atagatgagt
    17881 aaaaaggtag agcagctttg tctgtatctg cctttttatg ctatacttaa agtatgcata
    17941 gaaaaacagt gattgatttt aggattttgg gggagagata cacctttacc cagcctatca
    18001 aagagataaa aacgagaaat gtagcagaag tggcagattt gctggcacaa gtggaaagct
    18061 accaagagca aggttattat gtggtggggt atgtcagcta cgaggctgcc cctgcttttg
    18121 aggaaaaatt agcagttcac aaggatcccc tactgggaga gtacctgctt tactttactg
    18181 ttcatgatag ggtagagacc tcccctattc ctctgactta tgaggatatt gatttgccct
    18241 caaattggca ggaagtgacg tctgcagcag actatgaaaa ggccattgcc cagatacacc
    18301 atcacttgag gcagggagac acctatcagg tcaactacac cgtccaactc aagcaaaagt
    18361 taagtgccaa tccttttgcc atctacaatc gtatggtggt agagcaggag gcgggctaca
    18421 atgcctatgt ggaacatgac gagatggcag tgatttccat gagcccagag ctcttttttg
    18481 agcaaaatga tcgcgagttg acaacacgac caatgaaggg gacgactcag cgtggggtaa
    18541 ctgaccaaga agatcttgaa caggccagtt ggttggaaca ggatcccaaa aatcgctctg
    18601 aaaatatgat gattgtggac ctcttgcgca atgatatgaa ccgtatttct gaagttggga
    18661 gcgagcacgt ggagcgtctg tgccaggtag agcagtattc aactgtttgg cagatgactt
    18721 cgaccatcaa gagtcagttg cgagaggatg tggaccttgt tgaaatcttc cgctcactct
    18781 ttccttgtgg ttccataacg ggtgcaccga aaattgcgac aatggagatt atcaaggact
    18841 tggagcctca accgcgtgga gtctattgtg gaacgattgg tctcttgctt ccaaatggac
    18901 gacggatttt taatgtggcc attcgtacca ttcaacttca caaaggtcaa gccatttatg
    18961 gagttggcgg agggattact tgggatagca catgggaatc tgaataccga gaggttcatc
    19021 aaaaggcagc agttctttat cgtaaacaag cccgcttcca actgattacg acaggggaaa
    19081 tcagccagaa gaacctgctg tttgaagatc aacatctgga aagactgaga aaagctagtc
    19141 gttattttgc ctttcctttt gatgcagaag acttgggaca caagattgag gaagagtgtc
    19201 aggattgtga agctaatcaa gattaccgct tgcgaatcag ccttagcaaa tctggagaga
    19261 tagaagtcaa tcgtcaagta ttaacccctc tcagtacaag cttttgtcag gcccaagtct
    19321 gccttcagga agctgctttg aatcaatcct ttacctactt taaaaccact caccgaccgc
    19381 atttgagcct aggagaacaa gagaagattt accacaataa gtcaggaaaa ctgcttgaaa
    19441 cctctatagg aaatttggtt ctgaaaatcg ctggaaaact ctacacaccg cctatccgac
    19501 ttggaatctt gccaggaatt taccgtcagt atttgctaga aacaggacag gtagaagaga
    19561 aagtcttgac cttggcagat ttagcccaag cagaagctat ttacggctgt aatgcagtga
    19621 gaggcttgta tgaactaagc cttgaggaga actagatgaa actgatattt ttgcatgggt
    19681 tagggcagtc agcagagtct tggaaagaag ttcggaatct actgacagat tatccttctg
    19741 aggctatcga gttatttcct tctggagtta gtaactatca acaagctaag gagcgagttt
    19801 atcagcacct agctcaagag acagaacctt ttgttttgat tggattgtcc ttaggtgctg
    19861 ccctggcact agaactttcc agttacgatt taccaaatct tcgagctttg attctgtcag
    19921 gatgcccgct taaattagca gggaatatcc tattttatct tcaattgctg atttttaaac
    19981 tacttcccaa aagagtattt gaaaaacagg gagcagacaa gactcttatg gtcggagttt
    20041 ctgaggaatt gaagacactt gatttaacgg atatagcagg gacttgctcc tatccaacct
    20101 tactaatttg tggcagcaaa gataaaccga atcttagttc tatgaaagct ctccatagac
    20161 tgttaacaga ttcgcagttt cagatcattc ctgatggccc tcatgtcttg aataaggcaa
    20221 aaccaaagga gtttgtagaa aagaccagaa gtttccttga attgctgaaa taagtttgat
    20281 aaaataatat tgaaatcgat atcataatta tgggagaaag aaatgaataa acgtctattt
    20341 ttaaaaatga gtctggtgac gttgccaatt ttagccttgt tttcacaacc agttttggcg
    20401 gaagaaaaca tccatttttc gagctgtaag gaagcttggg cgaatggcta ttcggatatt
    20461 cacgagggag aacctggtta ttctgccaag ttagaccgtg atcatgatgg tgtggcttgc
    20521 gaattgaaaa atgctcctaa gggtgctttt aaagcaaaac agtcaacggc tattcaaatc
    20581 aacacaagtt cagcaacaac aagtggttgg gttaagcagg acggcgcttg gtactacttt
    20641 gatggaaatg gaaatctagt gaaaaatgca tggcagggaa gctattacct gaaagctgat
    20701 ggtaaaatgg cacagagtga atggatttat gactcttctt atcaagcttg gtattatttg
    20761 aaatcagatg gttcttatgc aaaaaatgca tggcaaggag cttattacct taaatcaaac
    20821 ggtaaaatgg cacaaggtga gtgggtttat gattcttctt accaagcatg gtattacttg
    20881 aaatcagatg gttcatatgc tcgcaatgca tggcaaggaa actactattt gaaatcagat
    20941 ggtaaaatgg ctaaaggtga atgggtttat gttgccacct atcaagcttg gtattatttg
    21001 acatcagatg gttcttatgc ttacagtaca tggcaaggaa attactatct aaaatcggat
    21061 ggtaaaatgg ctgtcaatga atgggttgat ggtggacgtt attatgttgg cgctgacgga
    21121 gtttggaagg aaggtcaagc aagtacagct tcttctagta atgatagcaa tagtgaatat
    21181 tctgctgctt taggaaaggc aaaaagttat aattcgttat tccacatgtc aaaaaaaacg
    21241 tatgtataga caattaactt ctgattttga taaattttca aatgatgcag ctcaatatgc
    21301 cattgatcat ttagatgatt aacagatact ggtaggcgaa aaaattcctt ataattcact
    21361 tacgcaaacc tttgcataag tgaatgaatt ttgatatact agtactgtaa gcattttcaa
    21421 ttactacaca atgaaaaagg agaatatgat gagtcaaaag attattggga ttgaccttgg
    21481 tggaacttct atcaaatttg caatcttaac aacagcagga gaaatccaag gaaaatggtc
    21541 aatcaagaca aacattttgg atgagggaag tcatatcgtt gatgatatga ttgagtctat
    21601 tcaacatcgt ttggacttgc ttggattggc agcagcggac ttccaaggca ttggaatggg
    21661 atcaccaggt gtggttgacc gtgaaaaagg gactgttatc ggtgcctaca acttgaactg
    21721 gaaaaccctt caaccaatca aacaaaagat tgaaaaagct ttgggcattc catttttcat
    21781 cgataatgat gccaacgtag cagctcttgg tgagcgctgg atgggtgctg gagataacca
    21841 accagacgtt gtctttatga cactcggtac tggtgttggt ggcggtatcg tcgcagaagg
    21901 caaattgctt cacggtgttg ctggtgcagc aggtgagctt ggtcacatca ctgttgactt
    21961 tgaccagcca atcgcatgta cttgcggtaa gaaaggctgc cttgagacag ttgcttcagc
    22021 aacagggatt gtcaacttga ctcgtcgcta tgccgatgaa tacgaaggcg atgcagcctt
    22081 gaaacgcttg attgataacg gagaagaagt aactgctaag actgtctttg atctcgcaaa
    22141 agaaggagac gaccttgctt tgattgttta ccgtaacttc tcacgttact tgggaatcgc
    22201 ttgtgctaac attggttcaa tcctaaaccc atcaacaatc gtcatcggtg gtggtgtgtc
    22261 agctgcggga gaattccttc tacaaggtgt tcaaaaagtt tacgatgaaa atagtttccc
    22321 acaagtacgc acatctacta aattagctct tgcaactcta ggaaatgacg ctggagttat
    22381 cggagcagca tcacttgtat tgcaataaga taatgagaga ggaaaatgct aacctagagt
    22441 tagtaatgtt cctcttttat tttttatgct atactagtta ggacaatgaa tgaggtgaaa
    22501 cgaaatgaca aaagcagata cgatttttaa agagaatatt gaacgcattc taaaggaagg
    22561 tgttttctca gagcaggctc gtcctaagta caaggatggg actgttgcta attctaagta
    22621 tgtaactggt gccttttccg agtacgactt atctaaagga gaatttccta tcacaacctt
    22681 gcgtcccatt gcaatcaaat tcgccatcaa agaagttctc tggatttacc aagaccagtc
    22741 aaatagctta gaagtgctta atgacaagta caatgttcac tactggaatg actgggaagt
    22801 tggagacacg ggaaccattg gtgagcgcta tggtgccgtt gttaagaaac acgacattat
    22861 caataagctt ctcaaacagt tggaaaccaa tccttggaac cgccgcaata ttatttcgct
    22921 ctgggattac caagctttcg aagaaacaga tgggctgctc ccgtgcgcct ttcagaccat
    22981 gtttgatgtc cggcgtgttg atggggaaat ctatctggat gcgaccttga cccagcgctc
    23041 caatgatatg ctggtggccc accacatcaa cgctatgcag tatgtggctt tgcagatgat
    23101 gattgccaaa cattttggct ggaaggttgg gaagttcttc tacttcatca acaacctcca
    23161 tatctatgat aatcaatttg aacaagctca ggaattgctc cgtcgggagc cgtcaaactg
    23221 ccaaccacgc ttggttttaa atgttcctga tgggactaat ttctttgata tcaaagcaga
    23281 agattttgag ttggtggatt atgaccctgt taagccacag ttgaagtttg acctagctat
    23341 ttaaaagaat agaaaaaaga agttgagaat aatcccaact tcttttgttt cttaacgtga
    23401 tacgcggcga cgagctgctt ttttacggtt ttcttcgatg aaagctgctt tttgctcttc
    23461 tggttcgatt actttctttt taaatgcgta tactgcacct gcaacggcag cgacagttcc
    23521 tgcgacacct gttacaagac ctttagcgaa tcctttagcc atgagtcttc ctcctttata
    23581 ttctcaatca gccagcctcc tcaagaggtc acatttttct gactgacctt tttgtgttat
    23641 aataatagta acgaaaaaat gggaattttt caaggaaaaa agatgaaaac aaaaataatt
    23701 gtgattgttg gaccgactgc tgttggaaag acggctctag ctattgaagt tgcaaagcgt
    23761 tttaatggcg aagtggttag tggagatagc caacaagtct atcgaggact tgatattggg
    23821 acggccaagg ctagtccaga agagcaggca gctgtttctc atcatttaat cgatgttaga
    23881 gagataaccg agtcttactc ggcttttgat tttgtttcag aagctaagat gactattgag
    23941 gatattcaca gccgtggcaa gctagccatt atcgccggtg ggactggact ttatatccag
    24001 agcttgctag aaggttacca cctaggtggg gagactcctc atgaggagat tttagcttat
    24061 cgagctagtt tggagccata ttcagatgag gaattagccc atttggtgga tcaagcaggc
    24121 cttgagattc cccagtttaa tcgtcgtcgt gctatgcgtg ccttagaaat tgcccatttt
    24181 ggtcaggatt tggaaaatca agagaccttg tatgaaccgc tgattatctg cttggatgat
    24241 gaacgtagtc aactttatga gcgtatcaac caccgagtgg atttgatgtt tgaggctggg
    24301 cttttggatg aggccaagtg gctgtttgac cattccccta atgtacaggc tgctaaagga
    24361 attggctata aggaactctt tccttatttc cgtggagagc agaccttgga ggaagctagt
    24421 gagagtctca aacaggcgac ccgtcgtttt gccaagcgtc agctgacctg gttccgtaat
    24481 cgcatgcagg tcacctttta tcagattgga gaatctggtg tacaagaccg cattttaagc
    24541 cagatagagg agtttttaga tgattgaaac ggagaaaaaa gaggagcgag tcctgctgat
    24601 tggtgtggaa ttgcagggta tggacagttt tgacctctcc atggaagaat tggctagttt
    24661 agcgaaaacg gcaggggcag tcgttgtaga tagctacaga caaaaacgtg aaaaatatga
    24721 ttccaagacc ttcgtcggct ctggtaagtt ggaagagatt gcgcttatgg tggatgcaga
    24781 agaaatcact actgtcatcg tcaacaaccg tctgacccca aggcagaatg tcaatctaga
    24841 ggaagttctc ggtgttaagg tcattgaccg tatgcagttg attttggata tctttgccat
    24901 gcgggctcga agccatgaag ggaagttcca agtccaccta gcccaactca aatacctctt
    24961 gcctcgcttg gttggtcagg ggattatgct cagccgtcag gcagggggaa ttggttcccg
    25021 tggtcctggt gaaagccaac tggagctgaa ccgtcgtagc gttcgcaatc aaatcacgga
    25081 tatcgagcgc cagcttaagg tggttgagaa aaatcgtgcg actgtcagag aaaaacgttt
    25141 ggagtctagc acttttaaga ttggtttgat tggttatact aatgctggga aatcaactat
    25201 catgaacatc ttgaccagta agacccagta tgaagcagat gagctctttg cgactctgga
    25261 tgcgacaacc aagagtattc atctgggagg caacctccaa gtaactttga cagataccgt
    25321 tggctttatc caagatttgc cgacagagtt ggtgtccagt ttcaagtcaa ccttggaaga
    25381 aagcaagcat gtggaccttc tggttcatgt tatcgatgct agcaatcctt accacgagga
    25441 acatgaaaaa acgattctct ccatcatgaa agacctggac atggaagata ttcctcactt
    25501 gacgctttat aataaagcgg atttggtgga ggatttcacg cctacccaaa cgccatatac
    25561 cctcatttct gccaagtctg aggacagtcg tgaaaacttg caagcattat tgctagataa
    25621 gattaaggaa atttttgaag catttaccct gcgagtgcct ttttcaaagt cctacaagat
    25681 tcatgattta gagagtgttg caattctgga agaacgtgat tatcaggaag acggcgaagt
    25741 gattacaggc tacatttccg agaaaaataa atggaggtta gaagaatttt atgactgata
    25801 ttaaaacttt ggctctaaag tatggaggtt atacaagtct ggacaaggtc tatctggatc
    25861 agcttctagc tggtaaaaca gagcaggagc agttaacact tattacacct ccgccaagtg
    25921 ttgttaatgc ctactttgca gaactctatc agaaaaaaag tcctcaggct gcgacggatt
    25981 attttgcaga actcagtcag gaactgaatc tttacaatac tgagccaagt ttcaccctag
    26041 aaaataagcc ttttattcgg cttaatctgt ctggtaaatc ctttggtttt tgttatgaga
    26101 gtgacggctt tggtcggatt ttctctgaaa atgaggagaa aatctcggat gacttgctct
    26161 ttgaaattgc gcaaattttc tcccatcaat tagtctttga agagtctggt aagatttaca
    26221 tgaagcctgt caaggacgag gaagttgtca gcctagaaag tctcacagct ttgactgatt
    26281 tggaaagatt ggctgatggt cgaaaacgtc tcaaaggcta cagccaagag gatttattgc
    26341 aagaagctgc tgctttttct ggcaaacgct atttccgatc ggaaaaccgc acagccatgt
    26401 tatatattga ttaattagaa agtatcgaat ggatattcaa tttttaggaa cgggggctgg
    26461 tcagccctct aaagcccgca acgtttcaag tctcgccctg aaacttttgg acgagattaa
    26521 cgaagtttgg ctctttgact gtggagaagg tacgcaaaat cgcattctgg aaaccacaat
    26581 tcgaccacgt aaggtcagca aaatctttat tacccatctg catggagacc acatttttgg
    26641 tttgccaggt ttcctttcta gccgtgcctt tcaggccaat gaagagcaga cagatttgga
    26701 aatctacgga cctcaaggaa tcaagtcatt tgtcttaacc agccttcgtg tgtcaggttc
    26761 tcgtctgccc taccgcattc atttccatga gtttgaccaa gattctctag gtaaaattct
    26821 tgaaaccgat aaattcactg tgtatgcaga ggagctggac cacactattt tctgtgttgg
    26881 ctatcgtgtc atgcaaaagg atctagaagg gacgctggat gctgaaaaac ttaaggctgc
    26941 tggtgttccg ttcggcccgc tttttggtaa aatcaaaaac ggccaggatc ttgttttgga
    27001 agacggaact gaaatcaagg cagcagacta tatctcagcg ccacgtccag gtaagattat
    27061 cactatttta ggagatactc gaaaaacgga tgccagtgtg cgtctggctg tcaatgcaga
    27121 tgtcctagtt catgagtcca cttatggcaa gggtgatgaa aaaattgctc gtaaccatgg
    27181 tcactcaact aatatgcaag ctgcacaagt agcggtagaa gcaggtgcca aacgcctcct
    27241 actcaaccat atcagtgccc gtttcctctc aaaagatatt agcaaactca agaaagacgc
    27301 tgccacaatt tttgaaaatg tccatgtggt caaagacttg gaagaagtgg aaatctagca
    27361 gtcacagaaa ggataagtat gcctactatt ctcattaccg gagctagcgg tggtctagct
    27421 caagaaatgg tcaaactcct gcccaatgac caactcatct tgcttggtag aaataaggaa
    27481 aaattagccc aactctacgg aaattattcc catgcagaat tgattgaaat tgatattacc
    27541 gacgattcag ccctagaagc tctggtaact gatctttatc tccgctatgg caagattgat
    27601 gtcttgatta acaacgctgg ttacgggatt tttgagggat ttgaccaaat tgctgataaa
    27661 gacattcacc agatgtttga ggtcaatacc tttgccctga tgaatctgtc tcgtcacctt
    27721 gcggctcgta tgaaggaaag cagcaaaggg catatcatca acatcgtcag catggcaggt
    27781 ctaatagcta ctggcaagtc tagtctttac tcagcgacca agtttgcggc tattggtttt
    27841 tcaaatgctc tgcgactcga acttatgccc tatggagtct atgtgacaac agtcaatcca
    27901 ggtccaatcc gaacaggatt ttttgaccaa gctgacccag atggaactta tcttaaatcg
    27961 gttgaccgct tcctgctaga accagatgca gtggctaaaa agattgtcaa gattatagga
    28021 aaaaacaaac gagaactcaa tctcccgatt ttgttgaacc tagctcataa gttttatacc
    28081 ctctttccta agctagctga taagttggca ggggaaactt ttaattataa gtaaaaagaa
    28141 ccaatgtgca ggttgttgct agcctacata ttggttctta tcatttctca actatcaaac
    28201 tgaacttctt ctaggagata gtctataaag cgttcgccca tcttagatag gctggttttc
    28261 tcatgctgga tatagaccag ctcgatcggg tcatcaatat ccagtgggat agaaacgata
    28321 ttgtctccgt ttaggttgct gttcaaaatc cctgtcgcaa tggtataacc atccaaacca
    28381 atcaagagat taaagagggt ggcacggtca ctgaccacaa tggatttctt gtggtgttct
    28441 tgagaaagaa tctcttctga aaagtagaag gagttgtgcg tcccttggtc atagctgagg
    28501 taagggaaat tctccaaatc agacagtttc accttgtctt tctttgccag agggttggtc
    28561 ttgctgacaa agatatgcgg ttgcgctgtg aagagatggt gggctagcag gtgattgtca
    28621 tccagcatct tggttaaaac atcacggttg taactgttta agaagaggac cccgacctca
    28681 ctgcggaagt tcttgacgtc gtcgataatc tcccaagtcc gagtttcacg aaggaagagt
    28741 tcgtatttct ccatatcgct tttcttgagc aaagagacaa aggcattgac cacaaaggca
    28801 tagtgttgag acgaaacgct aaatagttcg cggtgggcga caggattttt atagcgttcc
    28861 tccagaagct gggtctgctc gacaacctga cgggcataag agagaaactc catcccatca
    28921 cgggtcaagg tgattccctt gggattgcgg ataaagatct caatgcccat ttcattttcc
    28981 aaatctcgca ctgcattgga gagacttggc tgagtgataa agagttgctt ggctgcctca
    29041 ttcatggagc cagtttcgac gattttgata atataatgta attgttgaat tctcatgttt
    29101 ttattgtacc atacttgcct tagaatcgct aaagaagata ggaaaataag gagcattgtg
    29161 tgagtctctt cttgaaaaaa acacaaaatt ttgctagaat gagtcttatg aaaacattct
    29221 atgatgtgca gcaattcctc aaacgatttg gtattattgt ttacatggga aaacgcttat
    29281 atgatattga actgatgaag ttggaactat ctcggattta cgatgcaggg ttgatggata
    29341 aattagacta tctaaaggca gaagcagttc ttcgcagaga gcacaaggta gaattggatt
    29401 atattgagaa aaatggagaa aagaactaat ggttacttgg attttgtggg cacttatact
    29461 agcaatgttg gcgtggatgg gctttaacta tcttcgtatt cgccgtgcgg ctaaaattgt
    29521 ggacaatgag gagtttgaag ccttgattcg tacaggtcaa ttgattgatt tgcgcgaccc
    29581 agcagaattc cacagaaaac atatccttgg tgcacgcaat attccttcaa gtcagttgaa
    29641 aactagtctt gcagcccttc gtaaagataa acctgtcctt ctctacgaaa accaacgtgc
    29701 gcaacgagtt acaaatgcag ctctttactt gaaaaaacaa ggtttttctg agatttatat
    29761 cctttcttat ggcttggatt cttggaaagg gaaagtgaag actagctaac cttatttcca
    29821 aattagtcga atgataggat gaagatattg ttttatagta taattaattt gtataaaatt
    29881 taaagagttc ttagaacatg aaagaaaaga agaaaaatcc actttttgtg ggtatcttgt
    29941 cgattattct tggtttgtta tttccaattg taggcctgat tttgggtatc ataggattgg
    30001 tcttggctat ttcataccaa aaagagtcac aattagacta taaaatagaa aagattctta
    30061 atatcttagg aattgtgatt tctgtagtta actggattgt agcaatcgcc ttaatttttc
    30121 ggaaataaaa gaaaaaagct ttagttttaa agcttttttt gttttatcga ctcatctcta
    30181 aatagttttt aatgatttgt aactcttttt ggttcaaagg gtggtagtta ccttctgcta
    30241 aatctgggtt caatgtgaag tccccaaatt ggattctctt gaggttagtc accttaacac
    30301 caatcgagag gaacattttc ttgatttgat gaaattttcc ttctgaaatg gtgatagagg
    30361 cttgactgag ggaaggactt gctgatagaa tctctagtct tgcaggttta cagatagtgc
    30421 catctaaaaa gacaattcct ttttgaaagg tttggatatg gtcaggtgtt agaagtccat
    30481 taacctcaac ttgataagtc ttatcgacat gatattgggg atggaggagt tggaagccca
    30541 agggaccgtt atcggtcaag agaaggagtc ctgttgtatc tcggtccagt cggccgacgg
    30601 catagagctt gtcagactgg atgtcagatg gaagcaagtt catgacggtc ggaagttcct
    30661 tgtctttgtt ggctgtaacg acaccagcag gtttatgaag cataagatag gtgtgttcat
    30721 agccttgaat gattcgatcc tgaaaaagga gttcttgtag tcctgtatcg atattttgag
    30781 ctagggagcg ggctgggcaa ccatcgacta gaatttcccc tctgaggagt gcttgcttca
    30841 tggcctttcg gctaactttt tcttgggcta ataaattatc taaacgcata ctgtgcctat
    30901 cttatcataa gtcgcttgct ttgaaaaggc ttgacgtgaa aagaaaagcg gagtgaaaac
    30961 atttacactt gcttgagtta tgttatttat ttgaaattat ggtataatcg ttcagttaga
    31021 aaataaattt tgaatattat agaggaaatc atgacaaaat taagagaaga tatccgtaac
    31081 attgcgatta tcgcccacgt tgaccacggt aaaacaaccc tggttgacga attattgaaa
    31141 caatcagaaa cgcttgatgc acgtactgaa ttggcagagc gtgctatgga ctcaaacgat
    31201 atcgaaaaag agcgtgggat taccatcctt gctaaaaaca ctgccgttgc ttacaacgga
    31261 actcgtatca acattatgga cacaccagga cacgcggact ttggtggaga agttgagcgt
    31321 atcatgaaaa tggttgacgg tgttgtcttg gtcgtagatg cctatgaagg aaccatgcca
    31381 caaactcgtt tcgtattgaa aaaagccttg gaacaagacc ttgtcccaat cgtggttgtt
    31441 aacaaaatcg ataagccatc agctcgtcca gcagaagtag tggatgaagt cttggaactt
    31501 ttcatcgagc ttggtgcaga tgacgaccag cttgatttcc cagtggttta tgcttcagcg
    31561 atcaacggaa cttcttcatt gtcagatgat ccagctgacc aagaagcgac tatggcacca
    31621 atctttgaca cgattatcga ccatatccca gctccagtag ataactcaga tgagcctttg
    31681 cagtttcaag tgtcactttt ggactacaat gactttgttg gacgtatcgg tatcggtcgt
    31741 gtcttccgtg gtacagttaa ggttggggac caggttaccc tttctaaact tgacggtaca
    31801 actaaaaact tccgtgttac aaaactcttc ggtttctttg gtttggaacg tcgtgaaatc
    31861 caagaagcca aagcgggtga cttgattgcc gtttcaggta tggaagacat ctttgtcggt
    31921 gaaaccatca ctccgacaga tgcagtagaa gctcttccaa tcctacacat cgatgagcca
    31981 actcttcaaa tgactttttt ggtcaacaac tcaccatttg ctggtaaaga aggtaaatgg
    32041 gtaacttctc gtaaggtgga agaacgcttg caggcagaat tgcaaacaga cgtttccctt
    32101 cgtgttgacc caactgattc accagataaa tggactgttt caggacgtgg agaattgcac
    32161 ttgtcaatcc ttatcgaaac aatgcgtcgt gagggctatg aacttcaagt atctcgtcca
    32221 gaagttatcg taaaagaaat cgacggtgtt aaatgtgaac catttgaacg tgtacaaatc
    32281 gacactccag aagaatacca agggtctgtt atccaaagcc tttctgaacg taaaggtgaa
    32341 atgttggata tgatttcaac tggtaatggt caaactcgtt tggtcttcct tgttccagcg
    32401 cgtggtttga ttggatactc aactgagttc ttgtcaatga ctcgtggtta cggtatcatg
    32461 aaccatacct tcgaccagta cttgccattg attccagggg aaattggtgg acgtcaccgt
    32521 ggtgcccttg tttctatcga tgctggtaag gctacaactt actcaatcat gtctatcgaa
    32581 gaacgtggta cgatctttgt caacccaggt actgaggttt atgaaggaat gatcatcggc
    32641 gaaaactctc gtgaaaatga cttgacagtt aacatcacga aggcaaaaca aatgaccaac
    32701 gttcgttcag ctactaagga ccaaacagct gttatcaaga cgcctcgtat cttgacactt
    32761 gaagagtctc ttgagttctt gaacgacgat gagtacatgg aagtaacgcc tgagtctatc
    32821 cgtttgcgta aacaaatcct taacaaagca gagcgtgaga aagctaacaa gaagaaaaaa
    32881 tcagctgaat aagagctaga aagagagaaa gatggtctat ttagtcctag gaattttact
    32941 gctcctactc tatgtatttg cgacaccaga aagcattaaa gggacagtca atatcgtcgc
    33001 tatggtatgt attttagtgg cactcttgat tttattggtt ctatcttttc tgaaaatttt
    33061 tcaattacca acagaaatat tcctagcaat agccatgttg atcctagctt actttagtgt
    33121 gagagacatc acactcatgc cagtcaaaaa aagtaaaaga agataaaaaa gagagtttcg
    33181 gctctctttt tgctaggaat tttataatag tttgaattgc tttttagtta acaccatctc
    33241 ttttgcttgt atagtatgaa tgggaaaaac ttgaataaaa ttcgttaatg gcatctattt
    33301 tatatttggt tagtactggt tgcatattat tttgaaatgc gctttatttc aagtataggg
    33361 ggtaaattcc ctagtatagc ggtatttgtg gcttgattca taaccgattt ataaaatttg
    33421 tggacttttt aggaactttt taatttttca atggcttttt cataaaatga agtagcgttt
    33481 ttcttgttgt ctttggacaa gtggctatat gtattcatgg ttattgatat ttgagaatgc
    33541 cctagccttg tttgtatttc tttgtaaggc agtccggcat tgagcaagat actagcgtga
    33601 gtgtggcgga aagcgtggaa cgttaaacga gggcagtcag aaagttttaa atgtttctca
    33661 agtcttgacc taagcgccct agcgtctcta tattcgtcaa aggaattaga aaatactttt
    33721 tcataagtca agcctatttc tctaccaact tgcgcttgtc tatttttgta aagtcgcagc
    33781 atgagcaccg tcttattatc caagtcaatt accctaatac ttgatttagt tttaggggta
    33841 gtgacttctt tttcacagtt tagagtctta tttacgtcta gtgtaccgtt ttgtaagtca
    33901 atatcagacc atttgagggc taaacactca cgaatacgca aaccagtcgc taaaagcgtc
    33961 ttatatagca ccgtatcgta gaaatttttg taagtgtttg gcagctgctc caggtaagtc
    34021 aagaattttt ttaagttatc atcatccaga tatttcagtt tttgcccttc tttttcctta
    34081 cgacgtggca cgataatatc acgggctggg ttgaagggga taacttgcaa ggatacggca
    34141 tatttaagta ttcgtttatt tagcgagtta agttgcggat actcttggta acctttgcct
    34201 agttgattgt actcttttgc ccattgatta acttgctttt gaataactgg gggtgttagc
    34261 ttatctagtt tgtattctcc aaaggctgga agtacatagt tatttagtct actcttgatt
    34321 attgtaagtg ttgccttttt gactgtatgg cagtatgttt ccagccaatt ctccaccagt
    34381 tcggcataag tttttatctc caccgtctta tatactgtag acccgtcttt ttcaaaatct
    34441 atttgagcct gtagcgcttt ggctttgagt tctctcttgg ttcgccctga tatagtcgtc
    34501 ttgactttct tacctgttac ggtatcgata ccaagataga cgctagagcg atagactgta
    34561 gacccgtctt tttttgtgta ttctgttatt ttcatggttt tactcctctt ccatcagcag
    34621 gcaagcaatt agaaaagatt ttgagtttat accatgcgag gggctacgag aataccccta
    34681 ttttcgattt taattagtca gacggtaaat tgtaccagaa aagggaataa ggcagatatg
    34741 ggacttatat gggcttgttt gtgatgggtg tttttgatat aatgttttcg cctcctcaat
    34801 ttagagaagg gaggtgttat atatgctaga gttgttttcc ctttttctag ctccgttact
    34861 tgttaacgtc ttatctgagc ttttcaagct atggataaag agacgtagca agtagcattt
    34921 aaccctttaa gagggtagca aaaaacccca tcgctgtaac ggtggggctt tttagttaca
    34981 tatgctagaa gcactgtttt tccctttatg ctttcattct agcatacaag ccccaatatt
    35041 tcaagagttt tatttttttt gatattgttt agagcgcttt aagcgtttct aaaatgccaa
    35101 agtgttgaaa attgtctaaa ttataccgca ttatacttca ttagagagtt atagcctgat
    35161 gacagggtat tgacttacct tttttgtttt tctggataat atggtaaact agtcatgtaa
    35221 ccgcctgaat ctttcccgtt atttcagttc aggcgatatt agacaagtca gcagcagcta
    35281 gcttgtcttt tttttgttgt agaaaactag tgtagtaaaa agaaaagtgt accaacataa
    35341 taagccgata cactgatttt tttgtaaaat ataaaagccc ttaactgata gcaggttacc
    35401 caataggcaa cttacgaaaa atacgcagac atatcagaat tacaagcgac tttctaatat
    35461 aactctatcg tttttttatt aaaaatcaac ttttttgtcg ctttttttgt acttttttat
    35521 ttttattttg aaaataagtg taggcattga catttattcg ctgggaagta ttggcgatat
    35581 tgaacattac gattatggat ttttctaaga taatgctgac ggtttcgggt agggaaatag
    35641 tgttcccttc aataccgcta gaatggtagg ccatgcgtac cagtatatcg tccaggtagt
    35701 cttgagcgta gtccatgatt ctcctttctg taaaattaaa ctaaaatttt cccattggaa
    35761 tcaggagttt ttaacaatgc tgaaattact tggaaaagtg caagaagcat tggaataaaa
    35821 gtgaaactga atagtagata taaaaatcct tttctattat atcctgcata aaaatgatga
    35881 ccacctatcc aacctaaaac gatagctaat ataatgtaaa tccacttatt gacataatga
    35941 aatttaatat ttttagcttt ggttttggca tcaaaaattt ttgtttgtgg tatttcgtta
    36001 ttattgctaa tattattttt attatctttt ttatcgtcat tttttaagtt tg
//
LOCUS       DAVKWZ010000016        35901 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000016 DAVKWZ010000000
VERSION     DAVKWZ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35901
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.016"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(156..1790)
                     /locus_tag="VW854_000830"
     CDS             complement(156..1790)
                     /locus_tag="VW854_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262096.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMLICFSNIAIHFPFEEMPIYFSSMVAILLVIAFSTSLTAFYNVFYESK
                     DLASYRPYAFKESEIIIAKGLSVLLPALTGIVPILAYFLVLYIRLAPSLWLGLPLMLL
                     SLTLLFVSVALVMVVAVHFLAQTTVFRKYQSIFSNVMIGIGVLIPLIFVFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSIEAILGLLAWIALTVFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVITVLFTSAFLPYL
                     MMISLISKIRDSQIVPDIHPPYWLPLFFVGVFIAVVNNNITSLHSIALSLERENLDFL
                     KSLPFDFARYVKVKFWIIYAVQSFLPILTLLGLSLYLGLSILSMIYLLVVWIIASVIL
                     SCHHYFKDVKNLSTNWSSITDLVKRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAINLGVGALIVLLALAIFGYHYYLSRILAEIEKR"
     gene            complement(1771..2520)
                     /locus_tag="VW854_000831"
     CDS             complement(1771..2520)
                     /locus_tag="VW854_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0262097.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLTSSDLETSLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSAPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGSVEN
                     LRKDHPDQFLESIYLSLAGRKEEVADASQGH"
     gene            2699..4153
                     /locus_tag="VW854_000832"
     CDS             2699..4153
                     /locus_tag="VW854_000832"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEV0262098.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKEQGIQPMADVVL
                     NHKAAADNREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRSKSGIYLIQGDNKGWANKELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(4243..6861)
                     /gene="alaS"
                     /locus_tag="VW854_000833"
     CDS             complement(4243..6861)
                     /gene="alaS"
                     /locus_tag="VW854_000833"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEV0262099.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGQ
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNTSQLSSKLVAIVADNAEVGAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKIVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVDNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAIAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDVKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(6883..7368)
                     /locus_tag="VW854_000834"
     CDS             complement(6883..7368)
                     /locus_tag="VW854_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEV0262100.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSQISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(7643..7882)
                     /locus_tag="VW854_000835"
     CDS             complement(7643..7882)
                     /locus_tag="VW854_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262101.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTAILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(8143..9213)
                     /locus_tag="VW854_000836"
     CDS             complement(8143..9213)
                     /locus_tag="VW854_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV0262102.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYEAFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKLEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDPQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQDS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(9210..9983)
                     /locus_tag="VW854_000837"
     CDS             complement(9210..9983)
                     /locus_tag="VW854_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0262103.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIIAGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(9980..10786)
                     /locus_tag="VW854_000838"
     CDS             complement(9980..10786)
                     /locus_tag="VW854_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0262104.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(10767..11924)
                     /locus_tag="VW854_000839"
     CDS             complement(10767..11924)
                     /locus_tag="VW854_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0262105.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFEPEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(11983..12933)
                     /gene="murB"
                     /locus_tag="VW854_000840"
     CDS             complement(11983..12933)
                     /gene="murB"
                     /locus_tag="VW854_000840"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEV0262106.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARVVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESLSVAKMYAGGFTPCKR"
     gene            complement(13045..13797)
                     /locus_tag="VW854_000841"
     CDS             complement(13045..13797)
                     /locus_tag="VW854_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEV0262107.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWLEKKR
                     SLEKADALAYGLGHGGLELIFLGLISLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            complement(13807..14538)
                     /gene="budA"
                     /locus_tag="VW854_000842"
     CDS             complement(13807..14538)
                     /gene="budA"
                     /locus_tag="VW854_000842"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEV0262108.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            14680..15951
                     /locus_tag="VW854_000843"
     CDS             14680..15951
                     /locus_tag="VW854_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEV0262109.1"
                     /translation="MKKQSKYKEVVSYLKNGIESERFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEEHVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(15971..16786)
                     /locus_tag="VW854_000844"
     CDS             complement(15971..16786)
                     /locus_tag="VW854_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV0262110.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVDAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQVGKVDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKNEGTYQSYLEKAASLTEVEE"
     gene            complement(16943..17596)
                     /gene="phoU"
                     /locus_tag="VW854_000845"
     CDS             complement(16943..17596)
                     /gene="phoU"
                     /locus_tag="VW854_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV0262111.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRIKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(17608..18366)
                     /gene="pstB"
                     /locus_tag="VW854_000846"
     CDS             complement(17608..18366)
                     /gene="pstB"
                     /locus_tag="VW854_000846"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV0262112.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(18379..19182)
                     /gene="pstB"
                     /locus_tag="VW854_000847"
     CDS             complement(18379..19182)
                     /gene="pstB"
                     /locus_tag="VW854_000847"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV0262113.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKDFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(19193..20077)
                     /gene="pstA"
                     /locus_tag="VW854_000848"
     CDS             complement(19193..20077)
                     /gene="pstA"
                     /locus_tag="VW854_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV0262114.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSATVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(20067..20984)
                     /gene="pstC"
                     /locus_tag="VW854_000849"
     CDS             complement(20067..20984)
                     /gene="pstC"
                     /locus_tag="VW854_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV0262115.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(20984..21862)
                     /gene="pstS"
                     /locus_tag="VW854_000850"
     CDS             complement(20984..21862)
                     /gene="pstS"
                     /locus_tag="VW854_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEV0262116.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(22436..23740)
                     /locus_tag="VW854_000851"
     CDS             complement(22436..23740)
                     /locus_tag="VW854_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEV0262117.1"
                     /translation="MQFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDVMDYWSLDYPSQCASLQREILEDAVTMLAEGGHLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQTFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGEI
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(23741..24514)
                     /locus_tag="VW854_000852"
     CDS             complement(23741..24514)
                     /locus_tag="VW854_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEV0262118.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSSTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRSVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(24504..24782)
                     /locus_tag="VW854_000853"
     CDS             complement(24504..24782)
                     /locus_tag="VW854_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEV0262119.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(24782..25183)
                     /locus_tag="VW854_000854"
     CDS             complement(24782..25183)
                     /locus_tag="VW854_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEV0262120.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            complement(25393..26010)
                     /locus_tag="VW854_000855"
     CDS             complement(25393..26010)
                     /locus_tag="VW854_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEV0262121.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLTLVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSRRK"
     gene            complement(26000..26773)
                     /locus_tag="VW854_000856"
     CDS             complement(26000..26773)
                     /locus_tag="VW854_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEV0262122.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKENPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(26784..27521)
                     /locus_tag="VW854_000857"
     CDS             complement(26784..27521)
                     /locus_tag="VW854_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEV0262123.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIAEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKV"
     gene            complement(27526..28656)
                     /locus_tag="VW854_000858"
     CDS             complement(27526..28656)
                     /locus_tag="VW854_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEV0262124.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(28740..29129)
                     /locus_tag="VW854_000859"
     CDS             complement(28740..29129)
                     /locus_tag="VW854_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEV0262125.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIHITEE"
     gene            complement(29145..29528)
                     /locus_tag="VW854_000860"
     CDS             complement(29145..29528)
                     /locus_tag="VW854_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEV0262126.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            complement(29529..30317)
                     /locus_tag="VW854_000861"
     CDS             complement(29529..30317)
                     /locus_tag="VW854_000861"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEV0262127.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            complement(30310..31245)
                     /locus_tag="VW854_000862"
     CDS             complement(30310..31245)
                     /locus_tag="VW854_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEV0262128.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINIADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(31375..31551)
                     /locus_tag="VW854_000863"
     CDS             complement(31375..31551)
                     /locus_tag="VW854_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEV0262129.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(31691..32398)
                     /locus_tag="VW854_000864"
     CDS             complement(31691..32398)
                     /locus_tag="VW854_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEV0262130.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESNLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            32549..33577
                     /gene="queA"
                     /locus_tag="VW854_000865"
     CDS             32549..33577
                     /gene="queA"
                     /locus_tag="VW854_000865"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEV0262131.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGSHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQRIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHSIQEHYRFFS
                     FGDAMFIY"
     gene            complement(34126..35253)
                     /locus_tag="VW854_000866"
     CDS             complement(34126..35253)
                     /locus_tag="VW854_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_135354310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV0262132.1"
                     /translation="MNTSFVHAADGIQYVRDDTRDKEEGIEYDDADNGSIIVKVATKP
                     KVVTKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGHVTEKVTVD
                     RKEATDTVIKVPAKSKVEEVLVPFTTKYEADNDLSAGQEQEITLGKNGKTVTTITYDV
                     DGKSGQVTESTLSQKEDSQTRVVKKGTKPQVLVQEIPIETEYLDDPTLDKGQEVGEIG
                     KITLTTIYAVDERDGTIEETTSRQITKEMVKRRIRRGTREPEKVVVPEQSSIPSYPVS
                     VTSNQGTDVAVEPAKAVAPTTGWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGS
                     MKVNQWFQVGGKWYYVNTSGELAVNTSIDGYRVNDNGEWVR"
BASE COUNT        10763 a         8156 c         6775 g        10207 t
ORIGIN      
        1 actataaaca acaataatag aactatatta tatttcttat tcaaaacatt cctccctata
       61 tttttgatta ccaatcttaa tcatttacaa ctacattcta acaaactata aaagcgtttg
      121 tcgaattgaa tttatcaagc aagcaaccaa ctatctcatc ttttttctat ttctgccaat
      181 atgcgtgaca ggtaataatg atagccaaaa atagcaagag caagcaagac gataagagct
      241 cctactccca agttgatggc aaggataggg gagagagact gaaccaagaa tatgctccca
      301 attacaaggg ccatcaggat tgcactataa ataaacaata aaactatggc gactatgcca
      361 tttgaacgct tcaccaggtc cgtaatgcta ctccaattgg ttgacagatt tttaacgtcc
      421 ttaaagtaat ggtggcaaga aaggatgaca ctggcaatga tccagactac aagaaggtaa
      481 atcatcgaaa ggatggacaa gcctagatat agagaaagac cgagcaaagt cagaattggt
      541 aaaaaggact gaacagcata gataatccaa aatttcactt tcacataacg agcaaagtca
      601 aagggtaaac tcttcagaaa atcaagattt tccctctcca aggacaaggc aattgaatgc
      661 aggctggtga tattgttatt gacaactgct ataaacactc ctacaaaaaa caagggtaac
      721 cagtatggag gatgaatgtc tggaactatc tgagaatctc ggattttgga aatcagactg
      781 atcatcatga gataaggaag gaaagcactt gtaaaaagca ctgtaatcac gccagtcccc
      841 tgtcccaaga gggtgaggtg gtagcgtaaa accatacgga aaaagccctt tttagtagtt
      901 gaaatcctct ccttgctgcg acgttctttt ttgaccttct cctcactgtt aagcaggatc
      961 acgtcataaa aacgaggaag gaccttcttt ttggtcaggt aaagcaagaa aacagttaaa
     1021 gctatccaag cgagcagacc caatatggct tctattgaaa aaggctccac tgctattttg
     1081 taaaagatat gaagaggata aaggagaaat ggaatgtctc taactttgtc aacaatactt
     1141 ccaaaagtcg actgtagaaa gaagacaaat attaaaggta tgagaactcc tatcccaatc
     1201 atcacattcg aaaaaataga ctgatacttt ctgaagaccg tagtttgagc caagaaatgc
     1261 actgccacta ccatcactag agccacagag acaaataata aggtcaagga cagtagcatc
     1321 aaaggcaaac ccagccatag agaaggagct agcctaatgt agaggactag aaaataagct
     1381 aggattggta caattccagt tagagctggc aaaaggacag acagtccttt agcaattata
     1441 atctctgatt ctttaaaggc atagggccta taggaggcca ggtccttact ctcataaaag
     1501 acattgtaaa aggccgttaa agaagttgaa aaggcaatca ctagtaaaat agcaaccatc
     1561 gagctaaaat aaataggcat ttcctcaaaa ggaaaatgaa tggctatatt actaaaacag
     1621 atgagcatca agagactgga aaaaatgtaa gaacttagga ctctagcaga aacatttact
     1681 tttttcccag gattcttagc ctgcttcttt cgtagattag ccagattagc ttcttgagat
     1741 gaatagagga tattgatatc aactaatttt ttaatgacct tgagacgcat ccgcaacctc
     1801 ctcttttcta ccagcaagac taaggtagat actttccaaa aactggtctg ggtggtcttt
     1861 cctcaagttc tctacactac cacaatagat caaatgcccc tttttcaaaa tagcaatccg
     1921 atcacagact tgctcggcta cctcaaggac atgagttgaa aacaagaccg tcttgccttt
     1981 ttgtgcatgt tccttcatca tttgcttcaa atcaaaggcg gcctggggat ccaaaccagt
     2041 caagggttcg tccaaaaccc aaatatcagg agcagacaag agtgccccaa tgacaaagac
     2101 tttctgacgc attccgtgag aaagagtttc aataacctga tagcggtttt cagcaaaatc
     2161 aaaaacgttc aatagcctag ctagactagt ctccaagtca gagctagtca gatcatagga
     2221 tgaagcgatc aattcccaaa attcattggc cgttaagcgt aaaaataagt caggcgagtc
     2281 tgctacgtag ccaatctttc gtttgatcgc caagcgattt tccgataact cctgaccgtc
     2341 taccaaaata cgaccactgc tgggtgaaat gatactgact agggatttta tggtggtcga
     2401 ttttccagcc ccattatgac caatcaagcc cataatctct ccattttcaa tctgcaaatt
     2461 gagattactc aaggcctctt tatcaccata caacttacta acattttgaa attcaatcat
     2521 gggacaactc ctatctttat ctgtattata catcgctata ctagcacttt gatacaaaaa
     2581 aatcaatact ttattttaag tagttaaaat agtttccttc tatcttacgc aaacgtttgc
     2641 gccgagagat actttatgat agaataaaga acaagattga caagtaagag gaaacatcat
     2701 gcaaaatcaa acactcatgc aatactttga atggtatctg ccccacgacg gtcaacactg
     2761 gacgcgtctg gctgaaaatg ctccacacct agctcatctg gggatcagtc acgtctggat
     2821 gccaccagcc ttcaaggcaa ccaacgaaaa agatgtcggc tatggggtct atgacttatt
     2881 tgacttagga gagttcaacc aaaaagggac tgtccgcacc aagtatggtt tcaaagaaga
     2941 ctatcttcaa gccattcaag cccttaaaga acagggaatt caacctatgg ccgatgtagt
     3001 tctcaaccac aaggctgctg ccgataacag ggaagccttt caggttatcg aagttgatcc
     3061 tgtagaccgt acagttgaac ttggagaacc cttcaccatc aatggctgga ctagttttac
     3121 cttcgatggt cgccaagata cctataatgg cttccactgg cattggtacc acttcaccgg
     3181 tacagactac gatgccaaac gcagtaaatc tgggatttat ctgatccaag gggacaacaa
     3241 gggctgggct aacaaggaat tggtcgataa cgaaaacgga aactacgact acctcatgta
     3301 tgccgaccta gactttaaac atcctgaagt catccaaaac atctatgact gggctgattg
     3361 gttcatggaa acgactggtg tagctggttt ccgtttggat gccgttaagc atattgactc
     3421 tttctttatg cgcaacttca tccgcgatat gaaggaaaaa tacggtgacg atttctatgt
     3481 ttttggtgaa ttttggaacc cagacaagga agccaatctg gactatctcg aaaaaacgga
     3541 agaacacttt gaccttgtcg atgttcgtct ccaccagaat ctctttgaag ccagtcaagc
     3601 tggcgcaaac tatgaccttc gtggcatttt cacagatagc ctggttgaac tcaagcctga
     3661 caaggctgtg acttttgtcg acaaccacga tacccaacga ggacaagccc ttgagtctac
     3721 cgttgaagaa tggttcaagc cagcagccta tgccctcatt ttgttacgcc aagacggcct
     3781 tccatgtgtc ttttacggag actactatgg gatttcaggc cagtatgctc aagaagattt
     3841 caaagaaatc cttgaccgcc tcctagccat ccgaaaagac ttggcctatg gagaacaaaa
     3901 tgactacttt gaccatgcta actgtatcgg ttgggtacgt tcaggtgctg aaaatcaatc
     3961 cccaatcgca gtccttatct caaatgacca agaaaacagc aagtcaatgt ttgtcggtca
     4021 agaatggact aatcaaacct ttgtagattt acttggtaac caccaaggtc aagttacaat
     4081 tgatgaggaa ggttatggac aattccctgt ctcagctaga tccgtaagtg tctgggcagt
     4141 caataccatc taatagctca taataaccaa gctaggtcca agcggatttg gcttttttgt
     4201 attcacaaaa agacctaccc aaatggatag atctttactt gattacaatt tacctgctac
     4261 tgcatccaac aattcttgga tcttagcttg gttgcttcct cctgccatgg ccatgtctgg
     4321 tttaccacca ccacgtccat cgatgattgg tgctaattct ttgacaaggt ttcctgcatg
     4381 aaggtctttt gtcttgcttg ctacaaggac attgactttg tcaccgatag cggcaactag
     4441 gacaagaaga tcagagtagt ctttttgttt ccagttatct gcaaaagtac gaagggcacc
     4501 ggcatcggat acagacactt gactagcaat gtaacgatga ccgttgactt ccttaacatc
     4561 tttgaagata tcgcctgcgg ctgcagctgc ggctttttct ttcaactcag cattttcttt
     4621 ttgaagttga cgaagttgtt cttgaagtcc ttctaccttg tgaggtactt ccttgacttg
     4681 aggtgctttc aaggttgctg cgatagcttt aagagcatcc tcttgttcac gataggcttc
     4741 aaaggcttcc ttaccagtca ctgccaagat acggcgagtt cctgaaccga ttccttcttc
     4801 tttgacaatt ttgaagagac caatctcaga agtgttgtca acatgagtac caccacaaag
     4861 ttcaatagag tagtcaccga tagtcacgac acgaacttcc ttgccgtatt tctcaccaaa
     4921 gagggccata gctcccattt ctttagcagt gtcaatatcc gtttcaactg tcttcacttc
     4981 aagtgcttcc caaattttct cgttaacttg ctgttcaatc gcacgaagtt cctcagcagt
     5041 tactgcttgg aagtgggtaa agtcaaagcg aaggaattca acttcgttaa gagatcctgc
     5101 ctgtgttgcg tggtttccaa ggatattgtg aagggcagcg tgaagcaaat gagtcgcagt
     5161 gtggtttttc atgacacggt gacggcgatt gctatcaatt gccaaggtat attcttggtt
     5221 caaggcaagc ggtgcaagga cttcaactgt atgaagggct tgaccatttg gggctttctg
     5281 aacattggtc acagtagcca caatcttacc tgactcatcc aagatttgtc cgtagtcagc
     5341 tacctgtcca cccatttcag cataaaatga cgtttccgca aagataagag aggcagttcc
     5401 ttctgaaaca gctcctactt ctgcattgtc agcaacgata gctaccaatt tagaagacaa
     5461 ttggctagta ttgtagttga agacactttc tacagtgatg ttttgaagag tttcattttg
     5521 catacccatt gagccaccct tgacagctga cgcacgcgcg cgttcttgct gttctttcat
     5581 ggctgcttca aaaccttcac ggtctacagt cataccagct tcttcagcga tttcttcagt
     5641 caattcaact gggaacccat aagtatcata gagtttaaag acatcttgac cagcaatgac
     5701 agattgacct ttttctttca agtctgccac aatgccttgg gcaaagtgtt gacctgagtg
     5761 aagggtacgg gcaaatgatt cttcttcgct cttaacgatt ttctcaataa agtcacgttt
     5821 ctcaagcact tctgggtagt agctttccat gatttttcca acagttggaa ccaatttgta
     5881 aaggaaaggc tcgttgatac ccaatttttg accatgcata gaagcacgac ggagaagacg
     5941 acgaagaaca taaccacgac cttcatttcc tggaagggca ccatcaccga tagcaaatga
     6001 aagagaacga atgtggtctg cgataacctt gaagctcatg ttgtcgccat cttggtcata
     6061 aaccttacca gacaatttct cgacttcacg gataatcggc atgaagaggt ccgtttcaaa
     6121 gttggtctta gccccttgga taacggccac caaacgctcc aaaccagcgc ccgtatcaat
     6181 gttcttatgt ggcaattcct tgtattcgct acgaggaaca gcagggtctg cgttaaattg
     6241 tgacaaaacg atgttccaga tttcaatata acggtcgttt tcaatatctt ctgcaagcag
     6301 gcgaagaccg atattttctg ggtcaaaggc ttccccacgg tcaaagaaga tttctgtatc
     6361 tggtccagaa ggtcccgcac cgatttccca gaagttgtcc tcaattggaa tcaagtgact
     6421 tggatccact cccacttcaa tccagcggtt gtaagaatct ttatcgtctg gatagtaggt
     6481 catgtaaagt ttttcagcag ggaaatcaaa ccattcaggg cttgtcaaaa gctcataagc
     6541 ccaagtgata gcttcgtcac ggaagtaatc cccgatagag aagttcccca gcatttcaaa
     6601 catggtatgg tgacgcgcag tcttccctac gttttcgatg tcgttggtac ggatagcctt
     6661 ttgggcattg gtaatacgtg gattttcagg gataatggtc ccgtcaaagt atttcttaag
     6721 ggttgctacc ccagagttga tccacaaaag agttgggtca tttacaggaa ccaaacttac
     6781 tgatggttct actgagtgac ctttggtcgc ccagaaatca agccacattt ggcgtacttg
     6841 tgcactagat agttgtttca tattgtctcc ttattcactt gtttaatgtg attggctttc
     6901 cagcatttcc acatagtcaa tcgcgacaca gagggaaatg actaggtctg cataagcgtc
     6961 ttcaagaacc gttacggtat aggtagaggt cagatggaag agttccttct taatttccgc
     7021 aatcaactga tcgcgatcat ccagcaattt gaaattcaaa tcccagatat tgccctcgat
     7081 acgaagacct agattatcaa actcatactt atctcgccag aaggtcaact tcttacgaat
     7141 gacaaaactc gagccatccc gaagctgaat ctcaaaacga ggaagcaagg tcaagatttc
     7201 tttactgatc tgactgactt gttcaccagc cgcatcatag atggtaaaag ttttgggaat
     7261 cttaaaaaat gatccctcca cctgataggc aatttctccc ctgtcatcct tgatagcgaa
     7321 gcgttcgcct ccaagacgaa acttttgttt gacaagaaat gttttcatca acacctccaa
     7381 aaatcaaaag acaagctcat atcacgaagg gcgaaaaacc gcggtaccac cttcattcaa
     7441 tgaacttgtc attctcttgt tcttatgcaa ttgtatgatt gagtagcatg acttcctatc
     7501 ttagatggct cgcagcaccg ccatttctct ggactaagac aagtgaaaat caattctcaa
     7561 ctttcttatt ataacgtttt tttaagcttg cgtcaactgg aaatgatctc cgttgaatta
     7621 gaccaattcc ctacatctct gattactttt tcaggatata ttttttctta ctgccatttt
     7681 tctttttatc ccaaattttc atattactaa acacagctac tagaatattt ccaaatataa
     7741 aggtgcctat cacccaatat atggactcag ttgttaggta ttgtcgatcc aagccatcct
     7801 ttaaatggaa tagtatagca gtttggttaa caatcataaa ggttggccag aaactttttt
     7861 tgaaaaaagt agacattttc attatttgtt gccgctttct gtaaggttaa tactcaataa
     7921 aaatcaaaaa gcaaactagg aagctagctt caagctgtac ttgagtacgg caaggcaacg
     7981 ctgacgtggt ttgaagagta taggcttagt atactactag gcaagcaaat aagcaaataa
     8041 acaaataaac aactagaata gaaaaagata gggctctaaa aactgacttc tattccttaa
     8101 aaacgaacca gcttgactga ttcgtcttct tacgtttatc tcctacttcc gatacatttt
     8161 aaactgtagg aagaggtcgc tatatttccc tgtccattta tggtcaaatt tctcataaac
     8221 ttctaggtgt ttcatggttt caacatcggg atagaaggcc ttatcttcct ttgtttcctc
     8281 tgggagcaat tccttcgctg gtaggtttgg tgttgaatag ccgacatact ccgcattttg
     8341 gagagcattt tcaggtttca acataaagtt gataaaggca taggctgagt cttgattttt
     8401 aactgttttg ggaatgacca tattgtcaaa ccaaagattg ctggcctctg tcggtaccac
     8461 ataacgtaga ttttcatttt tttctaacat ttggctggct tcaccagaga aggtcacgcc
     8521 gattgcaaca ttattctgaa tcatgtagcc cttcatctcg tccgcaacga tagccttgat
     8581 atttggagtc agtttgtaga gcttatccac tgtctcttcc aactgctgcg gatccttgga
     8641 gttgaggctg tagccgaggg aattgagtcc tagtcccagc acctcacgcg ccccatcaaa
     8701 aagcatgata gaattcttat actccagctt ccaaaggtca tcccaatgct caggcgcttc
     8761 atctaccatg gtttcgttgt agacaattcc taaggttccc cagaagtaag ggatggagaa
     8821 tttattacct gggtcaaagg actggttgag aaactctggt ccgatatttt cgattccttc
     8881 aatttttgaa taatcaagcg gaaccaagag gtcttcgtcc ttcatcttgt taatcatgta
     8941 ttcacttgga atggcaatat cgtaggtcgt tccaccctgc tttatcttag tgtacatggc
     9001 ttcgttggag tcaaaagcct cgtactgaac ttgaattcct gtttcttctg taaactgagt
     9061 caagagttca ggatcgatat agtctcccca gttatagata accaattttt gactatctcg
     9121 actattgatt ttactatcta aatgagtcgc aattccccac aagacaagga taatcgctgc
     9181 aattcctgct aaaaatgaat agattttttt catgcttgct cctccttctc acgagagata
     9241 aagtaataac ctacaactag gataatacta aagagaaaga ctagagcaga aagggcattg
     9301 atttctagcg aaatcccctt gcgagcacga gagtaaatct cgactgatag ggttgaaaag
     9361 ccatttcctg ttacaaagaa ggtcacggca aagtcatcta acgaataggt gaaggccatg
     9421 aaataaccag caatgataga cggagtcagg taaggaagca tgatttcctt aaacatctga
     9481 aattgactag ctcccaagtc ataggccgca tgaatcatgt cgccattcat ttccttgagt
     9541 cgaggcaaga ccatcaagac cacgatagga atggagaagg ccacatgact agatagaacg
     9601 gtcaaaaagc caagtgaaaa cttgagttgg gtaaagagaa tcaagaagct agcaccaatc
     9661 ataacgtcag gcgcaaccat gaggatatta ttgagtgata gaaaggcttc ttggtatttc
     9721 ttacgagact ggtagatgta aatggcacca aaagtcccga taatggtcgc tatcaaggct
     9781 gataggaagg ccaagaaaaa tgtctgagcc aaaatcagca tgagtctccc atctccaaac
     9841 atggtttcaa agtgagtcca gctaaaacct gtaaagctat tcatatcatc accagcatta
     9901 aaggcatagc caatcaagta aaagataggc aggtagagga ccagaaagac cagtcccaga
     9961 taaaggttgg caaatttttt catcgttctc tcctttcctt agtcacccac atggtgatga
    10021 acatggtcag gatgagaatc acaccgatag ttgaacccat accatagttg tcattggtta
    10081 gaaaattctg ctcaatagcc gtccccaagg tgataacgcg gttcccacca atcaaacggg
    10141 tcagcatgaa gagactcaaa cttgggataa agaccgactg aaccccactt cgcacaccgt
    10201 tcatagatag agggaagatg acatgacgga aggtctccca cttagttgca ccaaggtcgt
    10261 aactagcatt gatgagatta ttatccatat cgtctaagac attgaaaatc ggcaaaatca
    10321 taaaggggag ctcgatgtag cttgcgacaa agataaagga aaaatcggta aaaagcaact
    10381 gttgtgaacc aattccgata aattccaaga attggttaat agagccattt tgaccaaaaa
    10441 tcccgataaa agcataggct ttaaggagca aattgatcca ggtaggaagg ataatcagca
    10501 tgagccagag ttgacggtgc ttgagacggg tcaaaaagag ggctgttgga tagctgataa
    10561 gcaaggtcac aaaggtcaca atccctgcat aaagcactga gttgaaactc attttgaggt
    10621 aggtcaagtt ttgtgacgca aagtaagatt tataattttc taaactaaac tggccttcaa
    10681 tgttgaaaaa ggattgtccg aaaatcaaga ccaagggtgc caatacaaag agggcaatcc
    10741 aaagcatgta gggcactaca aagagtttag agcttgtttt cttcatctct ttcctcctcg
    10801 atcgcattga tcagacctgc ttcttgctct tcgatttcca cgtattcttc gatacgagca
    10861 tcgaactctt cttcggtttc attgagacgc atgatgtgga tgtcttctgg ttcaaagtcc
    10921 agaccgattt cctcacccac gatggcctta cgggttgagt ggatcatcca ttcatttcca
    10981 agttcgtcat aggcgataat ttcataatgc accccacgga agagctgggt atcgacctta
    11041 acttggagct tgccttcttc aggaagggta atgcgcaagt cctctggacg aataacgacc
    11101 tcaacaggtt catttggctt catcccacca tcaaccgctt caaagcgttt gccgttaaat
    11161 tcgaccaagt agtcctcaat catggtacct ggcaagatgt ttgactcccc gataaaggtg
    11221 gcaacaaagt ggttgattgg ctcatcgtag atgtccacag gggttccaga ctggacaatc
    11281 tcgccatcat tcataacgaa aatccagtca ctcatggcaa gagcttcttc ctgatcgtga
    11341 gtgacaaaga caaaggtaat gcccaatcgt tgttgtaatt cacgcaattc gtactgcatg
    11401 tctgttctca atttcaagtc cagcgctgat aaaggctcgt ccaacaagac cacacggggt
    11461 tggttgatga tagcacgggc gatggccaca cgctgacgtt gtcctccaga aagtttgcgg
    11521 atggaacgtt tttcataacc ttccaactga accatcttga gaacttccgc tacacgctgc
    11581 tcaatttctt tcttatcaat tttacgcaag cgaagtggaa aggcaacatt ttcaaacaca
    11641 ttcatatgtg ggaacaaggc ataggattgg aagacggtat gtacgtcgcg cttgttggtt
    11701 ggaatatcat tgatacgaac accgtctagc atgatatctc ctgtcgtcgc atccagtaaa
    11761 cctgcaataa tgtttaggat agttgatttc cccgaaccag atgcacctag aagggtgtag
    11821 aatttccctt cttccaactc aaagttgatg tctttgagaa ccttggtgtt gctgtcttca
    11881 aaaactttag agacgttttt gaattcgata attggttttt tcaattggca taaattcctt
    11941 ctttttcata gattaaccga tcggggctct gtcaggtccc cactacctct tgcagggagt
    12001 aaaaccacct gcatacatct tcgctaccga taggctttca cccaagatcc ggacttctct
    12061 ttcaagcgta atacctgagt gttccttgac tttttcgata accgattgga tcaagtcctc
    12121 gtagtctttg gccgttccat ctgcgacatt gatcataaat cctgcatgct tttctgacac
    12181 ttctacgcca ccgatacgat agcctttcaa gccagcttct gaaattaact gacctgcaaa
    12241 atgcccgact ggacgcttaa agaccgagcc acaagatggg tattccaaag gttgcttgag
    12301 ttcacgtagg tgcgtcaagc ggtccatttc ctgcttgata acctgatggg ttcctggagc
    12361 tagggcaaat ttaactgaca agacaactgc accagactcc tgaatagctg aatggcggta
    12421 accaaaagcc aagtctttag cagacagggt ttcgatttct ccatccttgg tcaagacctt
    12481 acaagactgc aagatgtgag caatctcgcc accataggca cccgcattca taaagacagc
    12541 accgccaacg cttcctggaa taccacaagc aaactcaaag ccagttaaac tatgacggag
    12601 ggcaatgcga gttgtttcaa tcaagttagc cccagcttct gcttcaatgg tatagccatc
    12661 aacagaaacg ttattgagct tgtcacacaa gatgacaaat ccacgaatcc caccatcacg
    12721 aacgatgata ttgcttgcat tgccaagaac catccaaggg atattttctt ggttggcaaa
    12781 tttcacaacg cgagccaact caaaacgatt tcgtggaaag accaaataat cagcctctcc
    12841 acctactttt gtataactat agctatgcaa gggttcctta aaacggatat caattccttc
    12901 taagatttca agcatttttt ctcttacaga catgtcactc ttccttttac aaaattcatt
    12961 ccattatacc atttttagag acatttgacg accataaaaa taccttgttt ggattttgca
    13021 taagaaaaag aggttccccc ctttttatga ttttttacaa aagatttcct tggttccata
    13081 ggcgaccaga acgagctcca gtgctagaat cacttcaacc aagactggat ttgtcaacca
    13141 gcctacttgg aaaagagatg gtgccagatc aaagaaggca tgcaagccat aggctgctag
    13201 gagataaatc catttcttct ggcgaacagc ttggtaaacc caaactgtca aaagtaattg
    13261 gaaaccaagc gccaagattc gctcaaaacc aagcaaataa atctgccaga ccgaaagtga
    13321 ctgaatggtt tttaacatat tttcagacag taattgcata acctgtggat tctgagtttg
    13381 aactgccgaa agaacgatgt aaagattgag taaactaata aggcctagaa aaatcaactc
    13441 caagccacca tgccctaggc cataggccaa ggcatctgcc ttttccaaac ttctcttttt
    13501 ctccaaccat ttgaagaaaa caagacgagc cgtttcctca aaaaatgctg ccatggctag
    13561 gccatagata atatagataa gcggatgatc ttgcaagagg gcaatactac cgtctttttg
    13621 aggatgcaaa atcaagatat gcaccagttt ttccaaaatc tgcgaagaga caaagaaagc
    13681 gactgcaccc aagcccaaga cagctagatt aatctggtat ttctttttgg caaaccaaat
    13741 gcttcctatc agaaaagcta gcaacagcaa catggtaata atgatatgaa tggtcatttt
    13801 cttctcctat tctgcctttt caatatcttt tttcatctcg tcaacattga acttagcaaa
    13861 caagtattga cggtcttgga ctgggaaacg ttggtccaac tggtcaactg ctcccacctc
    13921 gataatgcct tccttgatga caaaatccat gacatgtcca ccgaaggtca aatcatctga
    13981 tatgaagtgc agatggtagc ctgccacact gaccccatgg aaaatctcag gcgtccagaa
    14041 accaacaata gtccccgcaa cattgtcacg actatattcc ggttgatggg ttgcgacatc
    14101 agcaaacttg gtatcgggtg ttgacttagg aatcatacgc acatgcatat gtgaaaattc
    14161 cccccgaatc ttgatagagc ggaaaagatt ttccccatca taataagact caattcgttc
    14221 tttcaattcc ttgtctgtca tctcaaagcg ctggcggaaa atgacctctg cctgatgcgg
    14281 taccactgca gcgtaaggaa taagggcatc tggtgacact tccacaattt ctggctggtc
    14341 tcctgaccct ttggcctgat aagccttgcc atccaagaca atcaattccc catcaatgga
    14401 atccaaggtt cccaaaccaa ggtcaccatg ttctagcaat tctcctactg tcatagtacc
    14461 accataaagg ccagccatca aggctccaag agtattgtat tgaaataatt tcactggttc
    14521 ctgcacgttc ttatccattc actttcttgt ctgttattct ataaatctta ctcctaagta
    14581 taccacattt gcccctagat gtgaacgaga gaaacgctct agacattgcc aagaaggaaa
    14641 aaaaagggta caatgtaaca aaatcaaggg aggtctggaa tgaagaaaca aagcaagtac
    14701 aaagaggtcg tttcctatct gaaaaatggt atcgagtctg aacgatttcc gacgggtagt
    14761 cgcctgcctt ctatccgtca actgagcctt gactttcact gcagcaagga caccattcaa
    14821 cgagccctgc tggaattacg gcacgaacaa tacctctatg ccaagcctca gagtggctac
    14881 tatgtattag aacaagggca acatcaagac ctagaaatcg aggttaccga cgaacatgcc
    14941 agtgcctatg acgatttccg actctgtgtc aatgaaacct tgattggacg agaaaactac
    15001 ctcttcaact actatgacaa tcaagaagga ttagaagacc taagacagtc cattcacaaa
    15061 ctcctatttg agcaagctct ctactgcaag gctaaccaac tagtactgac ttctggaacc
    15121 caacaagcct tgtttatcct ctctcaaata tcctttccta gacaagccaa ggaaatcttg
    15181 gtggaacagc caacctacca tcggatgaat cgcctcttga ttgcacaggg gctggactat
    15241 caaacgattg aacgaggcat tgatgggatt gacttggagg agctggaagg ccacttcaaa
    15301 acaggaaaaa ttaaattttt ctacaccatt cctagatttc actatcctct gggacattcc
    15361 tattctgagc aagacaaacg atctattctt aacttagctg ccaagtatga tgtctatatc
    15421 gtagaggacg attatctggg tgatttggac tccaagaagg gccaaacctt ccactatctt
    15481 gatacagagg agcatgtcat ttatatcaag tccttctcga ccagcctttt tcctgccctt
    15541 cgtattacag cactcattct tccaaatgct atcaaagaag catttgtggc ctacaaaaat
    15601 atcctagact acgacagcaa cctcattatg caaaaggccc tgtcactcta tattgacagt
    15661 caattgtttg aaaaaaatcg tttggctcgc ttgaccaatc atgaatctta ccaaaaacaa
    15721 atcgaggaaa ggataactaa aacaccttgt ccccttcctc attatcccct acacgatggt
    15781 ttattgctag acctgagaca gtatcctaaa atcgccagtc tcaaacacag tcaactgggc
    15841 ttggacttct ttgaagaggc ctatttaagc acctgtcctt atcaatttgc caaggtgtcc
    15901 ctagacaatc tggaaaatgt tttaaactat ttaaaagcag aattggaatg acatccaact
    15961 ctgctttttc ttattcttca acttcagtta gacttgctgc tttttcaaga tagctttggt
    16021 aagttccttc attcttaagt ttttggatga ccttgtctac cacttctttc aaatcatcac
    16081 tattttttct aagagcaacg gcattggcgt cgccgtcctt catcttcaag ctgacagttg
    16141 cgacagctaa gccagcgttt ttagcagcat aactaagtgc aacaggctca tccatatgaa
    16201 cagcatctac ttttccaacc tgcaattcat tgactgcttc acccatatta gttagggaag
    16261 ttaattgagc ttttggcaat tgttccttga ccattgattc tggaacagtc cctttttggg
    16321 ctgcaatatt agcactttct aggctagtta aatccttgta tttttccaca tcagccttac
    16381 gaaccaagaa actaatcttg ttttcatagt atgggattga aaaatcaaag acttcttttc
    16441 tctcgtcagt agcactaatt cctgcaactg ctaggtcagc cttaccagtt tgaagactgg
    16501 tcaaaacatt gtcaaaactc atgcttgaga tttccaactt aaccccaagt tcatcagcga
    16561 tagcctgagc catgtcgatg tctgcaccga ctacctggtt ctttccatca accaatgatt
    16621 gaaattcaaa gggtgcatag tcaggactgg ttgccacaac taatttccct ttttgtttga
    16681 tagcatccac agcagactga gaaccattag aacctgactg acaagctact aagaagaaac
    16741 ttgcaagaaa attacataaa acaaatatcc atttttttga tttcataaat aaacaacctc
    16801 tctgttaatt ttgtataatt ataacgatat ccaagttact tgtcaagtgt tttttaaatt
    16861 tttatctcaa aaatattttt tcgttcaaaa aaaggagcca tcagttgatt tcaagctcct
    16921 ttttatacag aattaaacta ttttataatt cgacaatctt acctgtttca aagtagacaa
    16981 cccattcaca gatattttta gcatagtcac cgatacgctc caagtaggaa ataacttgga
    17041 aataatcacg acccgtaaca atggcttctg gatttttctt aatctcttca gtcgcaaggt
    17101 cacggatagt ttcaaaatag tggttaattt gctcatccat ggaggccacc cggtatgcgt
    17161 cgtcaacaga accattaaga taaagatcaa gtgctgcttc aacaacgctt ttaacttcac
    17221 gtcccatttt tttaatttct tcctctacag ctgggatgcg ttcttccccc ttaatacgga
    17281 tggttgcctg ggcaatggct acagcgtgat cccccatacg ctccacatct gatacagcct
    17341 taaggacagt caagactgta cgcaaatctt gagagactgg ttgttggagt gcgatcattt
    17401 caaatgattt cttttccagt ttcacttcgt attcatttac ttctgcatca tcttcgatga
    17461 cctcttttgc caggtcacgg tcatgcgtga caaaagcacg taccgtacga ttgatttgtg
    17521 agagcacttc ttgtcccata gcgtagaact ggttatgtaa tttctctaaa tcttcttcaa
    17581 attgagatcg taacatcttt catctcctta tccaaatttt cctgtaatat agtcttccgt
    17641 ttccttgtgt tggggatcaa ggaacatctg cttggtatca ttaaattcaa tcaaatctcc
    17701 atctaggaaa aatcctgtct tatcagagat acgtgaagct tgctgcatgg aacgggttac
    17761 cagaagcatg gtgtacttgt cttttagacc atacaaggtt tcctcaattt taccagctga
    17821 aatcggatcc aaagccgaag ttggctcatc caagaggatg attttaggac tagttgccaa
    17881 gacacgggcc acgcagacac gctgctgttg accacctgac aatccaatag ctgaatcata
    17941 tagacgatcc ttgacctcat cccagataga ggcaccttgc aaggcttttt ctacggcttc
    18001 atccagaacc tgcttatcct taattccatt gatacgaagc ccgtagacaa cattctcata
    18061 gatagtcata gggaaaggat taggttgttg gaaaaccatt ccgatttcct tacgtaattc
    18121 aaccgtatct gtacgcggac tgtagatgtt gtgaccattg tacaccacgg atccagttgt
    18181 ggtcacctct ggattgagat ctcccatgcg gttgagagac ttgaggaggg ttgacttccc
    18241 tgatccagat ggaccaatca aggctgtaat ttccttaggt tggaaagata gggaaacact
    18301 attcaaagcc ttctttttat tataataaac ggacaggtct gatacctgta aaatcgcatc
    18361 tgtcatacgg tttcctttct aaccaaagtg accagataca tagtcattgg tggactgtag
    18421 cttggcattt tggaaaatag ttgcagtctt gtcatactca atcaaatcac ccaagtaaaa
    18481 gaagcctgta tagtcacttg cacgagcagc ctgctgcata ttatgcgtta caatgatgat
    18541 ggtaaagtct ttcttgagct caaacatggt ctcttctagt tgcatggtcg caatcggatc
    18601 caaggctgaa gctggctcat ccattaagag gatatctggc ttaacagaga tggcacgagc
    18661 gatacagaga cgttgttgct gaccacctga taaggtcaag gctgacttgt ggagatcgtc
    18721 tttaacctga tcccagaggg cagcctgacg aagggaggtt tctacgattt catctaggac
    18781 ttgcttatcc ttaactccag cacgttcatg cgcaaaggta atattacggt aaattgactt
    18841 agcaaatgga ttggggcgtt gaaaaaccat tccaatgtgt ttacgcattt cataaacgtt
    18901 gatttctgga cggttgacat caattccacg atagagaatc tgcccagtta ctttagcaat
    18961 atcaatagta tcattcatgc gattgagact gcgtaagtag gtagatttcc ccgatcccga
    19021 cggaccaatc aaagctgtaa ttttatttct ttcaaattgc atatcaatcc ccttaatgga
    19081 ttcattttta ccatagtaaa catggacatc cttagtagaa agggctactt tttcttcagg
    19141 aaaggtaagg atatgcttct catcccagtt atatgttgac atggcttctc ctttaggcag
    19201 cggttaattt cttgtgtaga tagcttccga acttacgagc tccaaagtta aaaatcagga
    19261 taaagatcag gagcacagtg gcagaacctg ctgatacaat ggttccatct ggaatagtgc
    19321 cttcactatt gactttccag atatggacag ccaaggtttc tgcttgacgg aagatagaga
    19381 tggggctagt cacactgagg atattccagt tagaccagtc aagagctggc gccgattgcc
    19441 ctgctgtata gatcagagct gcagcttcgc caaagatacg accagatgcc aagacgacac
    19501 ccgttacaat acctggaagc gcttctggaa taacaacatg aaccactgtc tcccagcgag
    19561 aaatcccaag agccagacca gcctcacgtt gggtatggtg aacgtgtttc aaactatcct
    19621 ctacattacg cgtcatctga ggcaagttaa agactgtcaa ggccaaggca cctgaaatga
    19681 ttgaaaatcc atactcaaac tggactacaa agatcaagta accaaagaga cccaccacca
    19741 ctgatggtaa agaggacaag atttcaatac aagtccgcac aaagttggta acaggacctt
    19801 ttttagcata ttcagccaag taaatcccag ctcccataga aagaggtaca gaaataatca
    19861 aggtaatgac caataggaaa aaggaattgt aaagctgaat gccaatccca ccacctgctt
    19921 gaaaagcaga agaccttcca gtcaagaaag accaagagat atggggcaag ccccgaacca
    19981 agatatagag aatcaaggaa gccaagattg tcacaatgat gctagcaatc gtatagagga
    20041 cagctgttgc aagtttatct aatttcttag cgcgcataat ttttctttcc tctttctttc
    20101 gtaatcaatt taatcacact gttaaaaact aaactcatca agagcagtac caaggccagt
    20161 gaccagagaa cattattatt tacagttccc atgacagtgt tcccaattcc catagttaat
    20221 atagaagtta aagttgcagc tggtgtggtc aaggaagttg ggataacagc tgagtttccg
    20281 acaaccatct ggatagctaa agcctcacca aaggcacgcg ccatcccaaa gaccactgca
    20341 gtgaaaatac cagaacgggc cgccttcaag atcacacgcc agatagtctg ccagcgagtg
    20401 gctcccatag cgaaactggc ttcacgataa taacgaggaa ccgcacgcaa gctatccgtt
    20461 gtcataaagg ttacggtcgg caaaatcatg acaaagagga cggaaatccc tgacaaaatc
    20521 ccaaaaccag tcccaccaaa gacactgcga acaaagggaa cgacgacttg caagccaata
    20581 aatccataca ctactgaagg aatcccaacc aggagttcaa tagctggttg caaaatcttc
    20641 gccccttttg gtgatacttc ggtcataaaa actgctgcac caatagcaaa gggtgttgcg
    20701 ataagggctg agagaatggt aacgataaag gaacccaaaa tcataggaag ggcaccaaat
    20761 tctttactag aaggattcca agttcctccc aaaagaaagt caaagatatt cacaccattg
    20821 acaaagaagg tcgacaagcc tttttgcgct acgaaaacca aaatcatggc cacaaggatg
    20881 actatcaaag aaagacaggc aaaggtcaaa ccttttccta atttctccag acgagaattc
    20941 tttgatggaa gcaacatttt cttagctaat tcttcttgat tcattattgt ctcccttcca
    21001 acactgtcac agttccggca gcatcttttt caaccttcat ttccttaatc ggaatatact
    21061 tcaatccttt gacaatccct tcttgggtct catccgagag aacaaaattg agaaattctg
    21121 cagccaactc attgggctgc cccaatgtat acatatgctc ataagaccac aagggccaat
    21181 tattgctact tatattttct ggacttaagt catagccatt caacttcatg cttttgaccg
    21241 aatcatctat ataggtaaga gataaataag agatagctcc tggacttttt gatacgattg
    21301 attttaccgc tccatttgaa tcctgctcct gactttgcat ggcagactga ccttccataa
    21361 tgacagtatc aaaggtagca cgagagccag agccggctgc ccgattgata acagagatgg
    21421 gtaagtcctt accaccaacc tctttccaat tggttacctc acctatgaag atttgacgaa
    21481 gttgctctgt cgttaggtta tcaacatcaa cctccttatt gacaatcaga gccaagccag
    21541 ctaccgcgac cttgtggtca acaagagcag aagcatcaat tccgtctttt tcctcagcaa
    21601 atacatctga gtttcctata tcaactgccc cagactgaac ctgggacaag cctgtaccag
    21661 aacctccccc ttggacattg accgtttttc caacatgaat cgtgccaaat tcatctgccg
    21721 ctacttcaac caagggttgc aaggcagttg agccaacagc cgttatggat tctccacgat
    21781 caatccagct agcacagcct actaaacaag ccgtcagcca aaaagcgata agagacagag
    21841 caagcttttt tctttttttc actgtttttc tcctcgaaaa taattatgaa tactgtgaat
    21901 tttttaagta gttctttatg agttgacgca tgaattctta ccaaatttct gcgcaattga
    21961 ttatttatat aatataggct atattactct ttcctaacct ccttttttca tatgtggata
    22021 aaatctcttg tctatccctt ccaccattgt cacccattat agtcatttcg tgtctctttt
    22081 tccccttttt aatgcaaggg aaattactct cctcagatga taatccaaaa gctagaaagg
    22141 tatctcaaac ctctctactc tcccagacta gtttacaact aaaaggaaaa gattctattt
    22201 tatgagaaat ctagtttaca agcggtaaga acgctaataa ctaaacttct tgtactcttt
    22261 gaaaatctct tcaaaccagt gttttgagct atctatggct agcttcctag tttgctcttt
    22321 gattttcatt gagtagtaaa actacatgta atggcaatca agatatcaag aatcatccta
    22381 ctaaaaaaat ccatactttc actataacat agaataagat atttgactag cattttcatt
    22441 tgaatctgag gccttttgga aaataatttt tcaaaacatt tccagtaacc tttgcaaagc
    22501 ccaagccatt gcctttaacc aaaacttggt accaaccatt tggcagactt tctgccagct
    22561 gaacgatttc tccagccgca tacttgacaa acgcttcttg gccaatttca accgactgtt
    22621 cgacctgact cggtttcaag gctaaaccaa gagcgaaact gggctcaaag cgtttcttct
    22681 taaaagtgcc cagatgcagt ccattgcgag caatcttgag cttccctaaa tctggcaaaa
    22741 gttctggcaa gagataaagc tggtctccaa aagtctgcaa gatacccggt agattgacct
    22801 tcaaatggtt ttgggcaaat tcctgccaca aggcaacttg ttcacggctg aggttactct
    22861 tacttgcctt aaatttagga gctggattgt tacccttaaa ctgtagatgg gcaacaaact
    22921 gaccctctcc cttaaactga tgaggataca tccgagccgt ttctggcagg tcaataccag
    22981 ctaccattcc attgatatgc tctactggca acaagtcaaa atcatactct tccagcaacc
    23041 aattgacaat ctcttcgttt tcctcgggtg cccaggtaca ggtcgaataa accagatgac
    23101 caccttcagc taacatggtc actgcatcct ccagaatttc tctttgcaag ctagcacatt
    23161 gactcggata atctaagctc caatagtcca taacatcagg ttgcttacga aacattcctt
    23221 caccagagca aggggcatca agaacgatta agtcaaaata gcctttaaag accttgacca
    23281 agcggtcggc agattcattg gtcaccacga catttgtcgc tccaaaacgc tccatgtttt
    23341 caaccaaaat cttagcccgt ttgcttgaaa tttcattgga aacaagtagc ccctcccctg
    23401 ctagataggc tgccagttga gttgatttgc cccccggtgc agcagccaag tccaagacct
    23461 tcataccagg actgggttgg gctacttgag ccaccatttg agcagcaggt tcttgcgaat
    23521 aaactaaacc tgtagcatgc tcaggcgatt tccctgaaac cttcccatag tggccccaag
    23581 gggtttgagt aatggcatca gaaaaggaaa gttgctcttc ttttaaggga ttgacccgaa
    23641 aggccgaaac cgcttcctcc tcaaaagagg caagaaaatc tcttgcctca tctcctagta
    23701 tctctttata tttttcaaca aatccttctg gaaattgcat ttaagttctt ttcctttcgt
    23761 aaatatagga ctgaatttcc tcctgcatct caagaggcac catcatgacc ggctgtctgg
    23821 tttgaaaatc aggagcttca ccaaaaaggg tcacaacccg atagcccaga ctttccccta
    23881 aaatactagc tgcggcataa tcccatggtt gcagataagt gagataggtc aacaaacgcc
    23941 ctgacaaaat cttggcaaaa ctaatggccg cacttccata gacacgaaca ccaagaaccg
    24001 atcggctcaa atcagccagc ccccattcat tggtttccag cataccacta ttccctgcaa
    24061 tgagaaaatc tccaagtggt ttagttttaa aaggagctag gggcctatca tttagacaaa
    24121 ctggaaattc cccaccaccg tggtaacaat cccctttgac cacatcataa atcagaccaa
    24181 actgtccctg accattttca aaataagcca tcataacagc aaaatcttcc tgctgggcta
    24241 caaaattatt ggtaccatca atgggatcaa tgacccaaac cttgccctct tgaaccgagg
    24301 ctcgcagaca accttcttca gcacaaatct tatcctcagg ataacgggac aaaatctcac
    24361 caaccaagag ttcctgaact tctttgtcca gtctggtcac caaatctgtt gaagaggact
    24421 tggtttcaac acgcaagtct tcctgcatat ggtcaagaat gtactggcct gctttcttaa
    24481 caagctcttt agcaaattca aatttacttt ccaagagaaa tctttccttc ccctttttct
    24541 ttggcagcct gaactgctcg gtaaagtgaa tagccactaa ccgtttcaaa ttctcgtccc
    24601 aaacgtttct cttcgctctt gcttggcacg accgctttga atcccttata ggagtccagt
    24661 aactttttag cctctacttt gccttcatag gcagcttcaa catcattaaa aaaagaaagc
    24721 actgaagcaa gttcttcagt gctccacgac aaatctagtg ggtaactata ctgtttgttc
    24781 attaactaat accagctctc attcttgctt cttttagttc ttgcttacga taactacgag
    24841 ggagaaaagc acgaatctca tcttcattaa aaccgatttg catacgcttg gcatcaataa
    24901 taattggacg acgcaaaaga ctaggatact gctcaatcaa atgaagcaat tccgataccg
    24961 aaatactctc tacatcaata ttcaattttt gaaaaatttt tgaacgagtt gaaatgatgt
    25021 catcagtacc attttcggtc aaggaaagga tgtgttgcaa ttcttttctt gttaaaggac
    25081 tggtcataat attgtgttcc acaaagggaa ccttatgttt ttctaaccag gccttagcct
    25141 tacgacatga tgtacagctc ggtgatagaa atagtgtaat catgcttttc tcttcttatc
    25201 tatactttgc tacttctatt atacaaaaaa ataaggcgct tgactaggga tttttagaaa
    25261 aaaagcctat tttttcaaga aaaataggct gtttgcgaac gattgacaca attggatttg
    25321 gttaattcac tcttaacgat ggttttaaac gatatatatt tttatatatg caaattaaaa
    25381 acatctttca tttcacttcc tacgactttt cagacacaga tacccaaaga agctttcata
    25441 gagggcaaaa aagaggagga aggcatgaag aaagaaggtc tctggcaaaa tcataataac
    25501 aggatccttg gctggatcaa aaagccaggt atcatctccc acaaagagaa tttgatggaa
    25561 aagagtaaag aattggtcaa aaccaatcaa aactccccca agtccaatca tcacaggtaa
    25621 gactactaga gtcaggagac tttttcgata aagagacaaa aagtcctttt tcacaatcct
    25681 attgacaaag acatagaaac ttggcagtgt cactagagct actagctgaa ccaaatgaaa
    25741 gagattcttg accactgcga aatggtgcag accagctgct gacgaacgaa aatcaggcat
    25801 ctgtaagacc tgactaaaag gattggtcag ataattcatc aagatatgaa aattgtattg
    25861 aatggtttct ggttttagat agactcgatt cgttaagttt agccactgaa tctccatagg
    25921 atagaaaatc caagccagat aaatggtcag aaggattgag agggagagga gaaagagcat
    25981 agagccccaa aaggtcaatt tagttttcat caaaatccca ctccgcaagg ctagaaacca
    26041 catgtgtcgg tgcgattggc aggccagcta cttcttctgc cttggtaaaa cctgtcgtca
    26101 ccaagagtgt tggaatgcca ttgtcaatcc cagcccggat atcagtcaaa taattgtccc
    26161 caaccatgat taactcttca cgttccaaac ctaagtgctc aaccgccttg tccataatga
    26221 tggcatttgg ttttccgata taaaccggct tcactcgtgt cgctacttca agcagcgtaa
    26281 tcagtgagcc agcacctggc aaaagaccac gttccgtcgg gatgttgagg tcaggattgg
    26341 ttccgataaa atgggcaccc ttttgaatag caagagttgc tgtggcaaat ttttcatagt
    26401 cgacttgcca atccagacca actaccacgt aggcagggtt ttccttgtct tccacataac
    26461 cagccgcctt gatggcttcc ttgagtcctg cttctccgac gacatagacg gtcttttcaa
    26521 gccccaaatc attcatatag tcgatggttg ccaaagtcgc tgtgtagaca gtcgatagag
    26581 gcgtatcgat attaaaattc tgagccaaca tctccttaac actctctgga gtgcgggttg
    26641 tattgttggt tacaaagaga tagggaatgt cccgcttttg caattcatga acaaaagtct
    26701 ctccagcagg gattcggtct ttccccttat aaatggttcc gtctaaatcg attaaatagc
    26761 ctttatattt catctatttc tccctaaacc ttttttattt cttgccaagt aatgattgct
    26821 tgggcattga taaccccatc acttgtaatt tcatgcttgc tttccagtcc agtccgttca
    26881 acagccgatg taatcacccc acctggtcga acttccttga catacttgag gttgattttc
    26941 ttgggaatat agtgggtcaa aaaatccgct cccatgacct caaaaatcca gtccaagtat
    27001 ttactgttat tgacatgacc attcatatcc aagtcgtaaa aacgaacatg gtaatccttg
    27061 ctgatcggct cttccaagga ttcatacttc ggtccacgga taagtttttt atcaaaatca
    27121 gactggtaag gagccacaat ctcaggttca acagcatgga cttttcgact gtcgcggtcc
    27181 atgagaacaa aggtcgccat catatggatg agctcctgcc ccgcttcatt ataaatagta
    27241 aagcgacggt agcaaaaaag tcgattgtag ctcaaggctt ccgtttcgat ggtaatttct
    27301 tccgcaaaac gaggcaaacg aaccacctca atatcatatt ctgcgataat ccagaccaga
    27361 ttatagtctt ccaaaatggc cttatcacta actcccagtt caatcgattg catccctgaa
    27421 acttgcagtg acagcaaaat cacatctgga agtttgatat gaccgttcat atcagccata
    27481 tcaaaaggaa ttttcatttt catttgataa gttaagccca tgatcctact ccaaaataaa
    27541 tcgttctgct acagtatctc ccaaaaagag acctctcttt gtcatgcgaa cgtggtcacc
    27601 ctcaatctgc atgaggcctt gttgaaccaa atctctgaca atttctccat aaagtccagc
    27661 aaaagactgt ccaaattttt cctcaaatcg cgccatggaa accccggatt tcttgcggag
    27721 tcccaagaac atttcttctt ccatttgctc cttttgactc aggtgatctt ctgtaataca
    27781 agcattgcct tcctcaaccg cactgagata atgacgaatg ggaccatgat ttttatagcg
    27841 tactccattg acataaccag atgcccctgc accaatacca tagtattcag cattgtccca
    27901 gtacatgaga ttatgacgac tttcaaaacc gggtttggag aaattagaaa tctcataatg
    27961 ctcaaaaccc gctcgctcca gctctgcaat gatgtactca aacatctccg cttctagttc
    28021 ctccttaggc agaggcaatt tcccacgtcg catccggttc ataaagaccg tatggttttc
    28081 taaaatcaaa ctatacaaac tcatgtgggg aatatccaat ccaatggctt tagccacatt
    28141 ttcctttact tgctccatgg tctgaccagg cagagcataa atcaaatcaa tggagatgtt
    28201 gtcaaaacca gccagtttca ggcgatcgat attttcataa atatccttct ccaaatgact
    28261 gcgcccaatc tttttcaaca tcttatcatc aaaggtctgg acacctagcg aaacacgatt
    28321 gacagccgaa tttttcaaaa cagctatctt atccgcatcc aaatcgcctg gattggcttc
    28381 aatggtcaac tcttccaaga cagacaaatc caagttttta gtcaagccat tcagtaacac
    28441 ctccagttgc ggagccgaca gggctgtcgg tgttccacca ccgatataaa gggttgacaa
    28501 cttttcaata tcataagaac gaaactcttc cagcagatgc tctaaatagc tgtcgactgg
    28561 ctgatttttg atgaagacct ttgaaaaatc acaataataa caaatctggg tacaaaatgg
    28621 gatgtgcaca taggctgacg ttggtttttt ctgcatagta attattatac cacaaagact
    28681 agattccaga taaaaatcac catccccaga tacatagtcc gtccggagat ggtgatggtt
    28741 tattcttctg ttatatgaat cacaatctct tctgagtcat caagagcttc ggctttttct
    28801 tgccattgtt ccttgagatt atttaattga ttttttgatg cttctgtcgc ttgaaaagca
    28861 taggatttag cttgagcaag tatactgtcc acagtgattt cacctgactc aacctgttct
    28921 tttgttttca gaacaaaatc tgtagcctgc tccttaactt ctgtcagttt ttcacagact
    28981 tgctccttgg catactccgg atcttctctc aaatcatcta gaaaatcttg agcctgactg
    29041 caaacttgtt tgcccttatc acttgttaaa aacaaggcaa gagctgcacc tgaaaccgtt
    29101 cctaaaagga ttgaggataa tttacccata aggattctcc ttttttattt tttgaaaaat
    29161 ttacttgcaa gacgaagagc tgacagactt gcaccagtct tgagtgtttt tgaaccagct
    29221 gatgaagctt tcttgctcaa gacacgcgca tggtcattga ggtctgaaac agatagagat
    29281 aaatctgcaa cagcactgaa gagtggatca atcgtagcca ccttgacatt gatatcatct
    29341 gccaagacat tgaccttagc caacaactca ttggtgtgat gcaaggtcac atccacatct
    29401 gaagtcaagg ttttaatcgt cttttctgtt tcatcgatga cacgaccaag cttttgtaca
    29461 gtaatgatca gatagaccaa aaagacaatc aaagctaggg caacaagaat atatgcaact
    29521 tctaacattt agttttcctc ctctgtaata tagtaagggg ccttctttcg attttgataa
    29581 ataacgatca ttataccgag accgataagg acaactgaca gccattggga cactcgaagt
    29641 ccgaagaaca tgagactatc tgttcgcata ccttcgatga ccatacgacc gaaaccatac
    29701 caaatcaagt aaaaggccgt gatatgacct cgtctgagac tcttccattt ccgtctaaaa
    29761 atcagaatca aggcaaagcc aagcagattc catagagact cataaaggaa agtcggttga
    29821 cggtagctcc cctcaatata catctggtca cggataaagc caggtagata atccagatta
    29881 tccactgttg caccataagc ttcttggtta aagaaattac cccaacgccc caaactttga
    29941 gcaatcataa cgctaggcgc cgcaatatct agaaaatccc aagtattgat gagtttacgg
    30001 tcagcaaaga tatagagcac aagagcccca gttatcaaac caccgtaaat ggccaaacca
    30061 ccattccaaa tggcaaaaat ctctcctaaa ttctgactat agtaatcaaa tcggaaaata
    30121 acatagtaga gacgagctcc taaaatagcc aagggaaagg ctactaagat aaaatctaaa
    30181 atatcgtctg gtatgatctt ctttctaggt gcttctttca tggtcaaata aaccgcaaga
    30241 atcaagcctg tcacaataca taaggcatac caacgaatgg ctaggggtcc tagttgaata
    30301 gcaattggat caagcatttt gcacctcatt tcgagcgatg agacttgtca gtcgttcgtc
    30361 gaacaaacgg gtcgcatcaa agcccatttc cttggcacga taattcatgg cagctgcctc
    30421 aatcacaaca gagatattac gacctgtttt aactggaata cgaatacgag gaatggctac
    30481 gccagaaact tcaagttcct ctgcattgtt tccaagacga tcaaaggtct tatgcgtatc
    30541 gtaattttcc aaatagacag caagctgaac ctgtgaagaa tccttgacag cactcgcacc
    30601 gtagagactc ataacatcga taataccaac cccacgaatt tcaatcaagt gtttcaaaat
    30661 ttcagctggt tcaccccaga gagtaatctc atccttggca aagatatcga cacggtcatc
    30721 ggctaccaaa cggtgaccac gtttgacaag ctcaagacct gtctcactct taccaattcc
    30781 actatctccc tgaatcaaga cgcccatccc ataaatatcc atcaagacac cgtgcacact
    30841 ggtacgttct gccaaacgag aatccagata gctagataac tctccagata aacgactggt
    30901 agctgcacgg ctggttaaaa tagcaatctt acattctcta gcagccttta acatctcctc
    30961 tggaaccacc aaaccacggg caacaatgac tgctggtgtc tcaggtagaa acattttttt
    31021 caaaacttca taacggctgt tggaaggcat gctgatcaga taagaccact ccttcatccc
    31081 caaaagttga atccgctctg gtgtatagta gtcaaaatag cctgtcattt caagaccagg
    31141 tcgcgtaata tccgctatat tgatttcctt ttcaagcaat tctggttcac catagacaat
    31201 gtctagtcta agcttttcaa tcacttcttt tactaaaacc gacatcattt cctccttcaa
    31261 atgagttctt ctctctatta tatcaaattg taggaggaag tttcagattt ttgcaggctt
    31321 tggagtattt atttaaaaga aaaaggacta gatttctcta gcctttcatt tcaattagaa
    31381 ttttttacgt ttacgagcta cttctgattt acgtttacgt tttacagaag gtttttcata
    31441 gaattcacgt ttgcgtgttt cttgaagagt accagcttta gtaaccgcac gtttgaaacg
    31501 gcgaagtgcg tcgtcaagag attcattctt acgtactact gttttagaca tttttttcac
    31561 tcccttcaag ttcaatatct atttcattct acacgtaaaa ggaataattg tcaagcaaaa
    31621 atgcggttta attttgattt tttcttccat ttatacaaga gttgaaagcg ttcaatagaa
    31681 cattgctttt ttatttttcg agtaagctaa gcgcttcagc atctgcgatg atggttacat
    31741 cagggtgatt ttggaggcta cttgcaggta ggttctcagt cactgggcca gatactgttc
    31801 cggcaatggc ttctgctttc gactcaccgt aagcaaaaag aataatagac ttggcatcca
    31861 aaatgttttt aatccccatt gaaatagctt gggttgggac gtcttcaatc ttggcaaaga
    31921 agcgtgcatt ggcttcgata gtagactggt caagttctac tagatgcgtt tgactgtcaa
    31981 atggagtacc aggctcatta aagccgatat gcccattgcg accgattccc aagatttgga
    32041 ggtcaactgg atggtcagcc aaaatttggt tgtagcgttc aacttcagct tcagcattgt
    32101 ccttaacacc acgaggcaag aaactttctt taaatggttt ttggttgaac aagttttctt
    32161 gcatgaagta acgataagac tgtggattgt ccccatcaag gcccacatac tcatcaaggt
    32221 tgacactggt tagatttgaa aaatcaaggt tactctcaac aatttccttg taaaattcaa
    32281 gtgggctact tcctgtcgca agtcctagag tttgagctcc attagccaat ttttccttca
    32341 aaatctcaaa agcaactttt ccaccttgaa cttggttttc aactttaata actttcatct
    32401 tttcatcctc catttctgtc tatatttatt atatggtata gaccaatttt tgtcaagtga
    32461 aaacgattta atttctaaaa aaagagcgtc caaacttgaa acgtgttata atggatagga
    32521 ttagaactta gaaagaatga acagatatat gaatacagct gattttgatt tccacttgcc
    32581 agaggaattg attgcccaaa cgccccttga aaaacgtgat gcctccaaac tcctcatcgt
    32641 caaccgtgag acaggagaaa tgcaagataa acacttccac tctattattg atatgctgga
    32701 acctggtgat gcccttgtca tgaacgacac ccgagttctc cctgcccgcc tctatggtca
    32761 aaaagtggag acaggaagtc atgtggaact tctcctcctt aagaacacta gtggagacga
    32821 gtgggaagtt ctggctaaac ctgccaaacg cctcaaggtc ggtactcgta tcagctttgg
    32881 tgatggccgc ctcagcgctg tcgttacaga agaattgacc cacgggggac gcattgtccg
    32941 ctttgaatac caaagaattt tcctagaagt cttggaaagt ctgggagaaa tgcctctgcc
    33001 accttatatc cacgaaaaat tagatgaccg tgaacgttat caaaccgtct acgccaagga
    33061 aagcggctct gctgcagcac cgactgctgg tcttcacttc accaaagaac tgctggcaga
    33121 aatccaagct aagggtgtcc atctagtcta tctgactctc catgtcggac tcggaacctt
    33181 tagacctgtt tctgtggata atctggacga acacgaaatg cactcagagt tctatcaact
    33241 ttctgaggaa gctgcggcca cccttcgctc tgtcaaagaa aatggtggtc gtgtcatcgc
    33301 tgtcggaacc acttctatcc gtaccttgga aactattggt tccaagtttg atgggcaaat
    33361 ccaagcagat tctggttgga ccaatatctt tatcaaacct gggtatgagt ggaaggtcgt
    33421 ggatgccttc tcaaccaact tccacctgcc aaaatcaact ctggtcatgt tggtttctgc
    33481 ctttgcaggc cgtgaattag tcttagatgc ctaccaccat tccatccaag aacactaccg
    33541 cttcttcagt tttggtgacg ccatgtttat ttattgagaa agaatttctc taaatcttct
    33601 aataccaata aatcgctaag atattatttc aaagaacatc tacaattgaa actctagcta
    33661 gctgtagaag acgcctagta cattgaaatt aaaatgcaag acacatgagt agcaccatat
    33721 ttgttgctct tatctgtcct cgcaagagac tattatgagt tatttcagaa tcattcacta
    33781 ctttgaccct gactctcctt agtctcaaaa tcaaagactt atactcttca aaaatctctt
    33841 caaaccgcgt caacgtcatc ttggattata tatgtgactg acttcgtcag ttctatctac
    33901 aacctcaaag cagtactttg agcaacctgc gactagtttt ctagtttgct ctttgatttt
    33961 cattgagtat taaacaaaaa gtgaacaaag ctgaattcta atgtacagaa gactaggctt
    34021 gttcactttt ttatagtcgc tataagatga ccttatctat agctttttat atataattat
    34081 atattcagac atactattat caattttgtc gcagggagga atctgttaac gcacccattc
    34141 accattatca ttgactctat agccatctat acttgtattg accgctaact cacccgatgt
    34201 atttacataa taccatttac caccaacttg gaaccattga ttgactttca tagaaccgtt
    34261 gctgttgagg tagtaccatg aaccattaac ttgtacccaa cctgttgcca tggaaccatc
    34321 agtattataa aaataccaca taccattttc ttgtttccag cctgttgttg gagcaactgc
    34381 tttagctggt tctactgcta catctgttcc ttggttagat gtaacagata caggatacga
    34441 aggaatagat gattgctcag gaacaacaac tttttcaggt tctctcgtcc ctctccttat
    34501 acgtcttttt accatctctt tagtaatttg acgagaagta gtttcttcaa ttgttccatc
    34561 acgttcatct acagcataga ttgtagtaag agtaatttta ccaatttctc ctacttcttg
    34621 acctttatca agagttgggt catcgagata ttctgtttcg attggaattt cttggacaag
    34681 aacttggggc ttggttcctt ttttaacaac tcttgtttga gagtcttctt tttgacttaa
    34741 agtactctca gttacttgtc cactctttcc atctacatca taagttatcg ttgtaactgt
    34801 tttcccattc tttcctagag taatctcttg ctcctgtcct gcagaaaggt cattgtctgc
    34861 ttcatattta gtagtaaatg gaacaagaac ttcttcaacc ttgcttttag ctggaacttt
    34921 gataactgta tccgtggctt cttttctatc aacagtaacc ttttcggtaa catgaccagt
    34981 ctctggatta acatcgtagg tccttgtcgt agttacatag ccatcctctc catcaattgt
    35041 aacaggattt tcactacggt cttttgtttc atctttttca taacgaattc gcgtacttga
    35101 aattttcttg gttactacct taggtttagt cgctactttt acaataatac tcccattgtc
    35161 agcgtcatca tactctattc cctcttcttt atctctagta tcatctctga catattgaat
    35221 cccatcagca gcatgaacaa aacttgtatt cagattcctc ctaaaaataa agttagcccg
    35281 attaccgcag aaccaaaaat ctttccgagt ttacgtaatg catagcgctt attagtatta
    35341 gattttgcca ttacatccta cttctagtat agcatctttt ctatcaaacg ttaaacagta
    35401 tactttatat atcagataga cttagaatga tatattgatt attgaactaa cactttaact
    35461 atatcgtaat caatctcata tataaaggat tgcagacatc ttatctaaat acatgctaat
    35521 atatttagat acaaacattc caacttgata attttcactc atctttcatc attccttata
    35581 caactatgca gtataaatag aatagttttc tcatcagaat gagactattt taatattaga
    35641 taatttgata gaaaaaaaca taaataaaat tggttgaatt taattcccta actcgataga
    35701 agattctatt ttaaatttaa caacagctag gattacacta taaaaagcac attaccacct
    35761 gttcgactgg agaatctagt cgattagctc cctcttagtt acaagcttaa agcgattttt
    35821 taactaatac taaaccagat taaagaagga actagaacat ctgtcttgta ctaatcaata
    35881 tgctatttta aaattgcaaa t
//
LOCUS       DAVKWZ010000017        33570 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000017 DAVKWZ010000000
VERSION     DAVKWZ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33570
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.017"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(223..585)
                     /locus_tag="VW854_000867"
     CDS             complement(223..585)
                     /locus_tag="VW854_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEV0262133.1"
                     /translation="MLEVGLNFLISLFTFTFDILYPIVKVDHTDDYSHGAVKLSVSLV
                     DKDAMKKISVTVIGYFEINIDENITDILYVNGTAILYPYLRSIVSTVSAIDSSEAMLL
                     PIINVLELLDKSQPFEEE"
     gene            599..673
                     /locus_tag="VW854_000868"
                     /pseudo
     CDS             599..673
                     /locus_tag="VW854_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(900..1058)
                     /locus_tag="VW854_000869"
                     /pseudo
     CDS             complement(900..1058)
                     /locus_tag="VW854_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(1331..2020)
                     /gene="rplA"
                     /locus_tag="VW854_000870"
     CDS             complement(1331..2020)
                     /gene="rplA"
                     /locus_tag="VW854_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEV0262134.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(2228..2653)
                     /gene="rplK"
                     /locus_tag="VW854_000871"
     CDS             complement(2228..2653)
                     /gene="rplK"
                     /locus_tag="VW854_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEV0262135.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            complement(2776..3492)
                     /locus_tag="VW854_000872"
     CDS             complement(2776..3492)
                     /locus_tag="VW854_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEV0262136.1"
                     /translation="MKKRYVILSGLLALTLAACSQEKTKVEENTQKTEQSSQPEGTVG
                     SKSQASSQKKAEVSNKGSYYSIQGKYDEIILANKRYPLSKDYNPGENPTAKAELLKLI
                     AAMQAEGYPISDQYSGFRSYETQAKLYQDYVNQDGKEAADRYSARPGYSEHQTGLAFD
                     LIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            complement(3510..3971)
                     /locus_tag="VW854_000873"
     CDS             complement(3510..3971)
                     /locus_tag="VW854_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV0262137.1"
                     /translation="MCLICQRIELIKAGQNPYFVKELETGYVVIGDYQYFKGYTLFLA
                     KDHVTELHHMETSVKLRFLEEMSLVQEAVAKAFEAEKMNIELLGNGDAHAHWHLFPRR
                     AGDMKSHGLNGRGPVWWVPWEEMAAEDCQVQSPELEEMIKILSHELEKYLA"
     gene            complement(3973..5010)
                     /locus_tag="VW854_000874"
     CDS             complement(3973..5010)
                     /locus_tag="VW854_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEV0262138.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDPRTVVTDTGFIKSRHLDDKVSAAILLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVVATERM
                     VDAYLKSEAVS"
     gene            complement(5108..6433)
                     /gene="brnQ"
                     /locus_tag="VW854_000875"
     CDS             complement(5108..6433)
                     /gene="brnQ"
                     /locus_tag="VW854_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEV0262139.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(6604..6696)
                     /locus_tag="VW854_000876"
     CDS             complement(6604..6696)
                     /locus_tag="VW854_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV0262140.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            complement(6739..6891)
                     /locus_tag="VW854_000877"
     CDS             complement(6739..6891)
                     /locus_tag="VW854_000877"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV0262141.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLEGAHNLPLSQ
                     LADSYD"
     gene            complement(7020..7097)
                     /locus_tag="VW854_000878"
                     /pseudo
     CDS             complement(7020..7097)
                     /locus_tag="VW854_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(7098..7676)
                     /locus_tag="VW854_000879"
     CDS             complement(7098..7676)
                     /locus_tag="VW854_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEV0262142.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMVKNPWFEAEVVPDLKKRIKTIL"
     gene            complement(7730..9130)
                     /gene="pepV"
                     /locus_tag="VW854_000880"
     CDS             complement(7730..9130)
                     /gene="pepV"
                     /locus_tag="VW854_000880"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEV0262143.1"
                     /translation="MTAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTVP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKIRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVVSVSLSEHGHTPHYVPMEDPLV
                     QTLLNVYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            complement(9143..9748)
                     /locus_tag="VW854_000881"
     CDS             complement(9143..9748)
                     /locus_tag="VW854_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEV0262144.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDEKLEPSYRLPVDEIIEKR"
     gene            9919..10719
                     /locus_tag="VW854_000882"
     CDS             9919..10719
                     /locus_tag="VW854_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV0262145.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINHWNQKHTD
                     NPATIDFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEKFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            complement(10897..11730)
                     /locus_tag="VW854_000883"
     CDS             complement(10897..11730)
                     /locus_tag="VW854_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV0262146.1"
                     /translation="MNHKIAILSDVHGNATALEAVIADAKNQGASEYWLLGDIFLPGP
                     GANDLVALLKDLPITASVRGNWDDRVLEVLDGEYGLEYPKEIQSMRMTQFLMERMDPA
                     TIVWLRSLPLLEKKEIDGLRFSISHNLPDKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSQGQQIINPGSIGMPYFNWEALKNHRAQYAVIEVEDGELVNILFRKV
                     AYDYEAELELAKSKGLPFIEMYEELRRDDNYQGHNLELLASLIEKHGYVEDVKNFFDF
                     L"
     gene            complement(11720..13564)
                     /gene="uvrC"
                     /locus_tag="VW854_000884"
     CDS             complement(11720..13564)
                     /gene="uvrC"
                     /locus_tag="VW854_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEV0262147.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDETYFKSMAQEVSDFLKGQDNKIIDDLKSKMAVAAQSMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDSKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMKHFKSLTKIKEA
                     SVDEIVEVGVPRVVAEAVQRKLNPQGEALPQVAEERVDYQTEGNHNEP"
     gene            complement(13604..14305)
                     /locus_tag="VW854_000885"
     CDS             complement(13604..14305)
                     /locus_tag="VW854_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEV0262148.1"
                     /translation="MRWLFRLIGAFFFFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            complement(14384..15616)
                     /gene="murN"
                     /locus_tag="VW854_000886"
     CDS             complement(14384..15616)
                     /gene="murN"
                     /locus_tag="VW854_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEV0262149.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTIGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSDYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            complement(15621..16841)
                     /gene="murM"
                     /locus_tag="VW854_000887"
     CDS             complement(15621..16841)
                     /gene="murM"
                     /locus_tag="VW854_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEV0262150.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            complement(16953..17732)
                     /locus_tag="VW854_000888"
     CDS             complement(16953..17732)
                     /locus_tag="VW854_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEV0262151.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKCEKTFIAGLSMGGYGCFKLALATNRFSHAASFSGALSFQDFSPESQNLG
                     SPAYWRGVFGEIKDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLVVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            complement(17801..19462)
                     /locus_tag="VW854_000889"
     CDS             complement(17801..19462)
                     /locus_tag="VW854_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV0262152.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEEYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            complement(19637..21859)
                     /gene="recJ"
                     /locus_tag="VW854_000890"
     CDS             complement(19637..21859)
                     /gene="recJ"
                     /locus_tag="VW854_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEV0262153.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQNQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFITFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDLPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            22136..22894
                     /locus_tag="VW854_000891"
     CDS             22136..22894
                     /locus_tag="VW854_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV0262154.1"
                     /translation="MALVEFKNVEKYYGDYHAFRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLETVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKAVLENVT
                     LAPIEVLGIDKKEAEKTAQKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLAHDGMNMIIVTHEMGFAREVADRIIFMADGEVL
                     VDTTDVDNFFDNPSEPRAQQFLSKIINHESDKVK"
     gene            22933..23697
                     /locus_tag="VW854_000892"
     CDS             22933..23697
                     /locus_tag="VW854_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV0262155.1"
                     /translation="MISLFGLAAAKPVQADTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLKFK
                     FVELGSYPELITSLHAHRIDTFSVDRSILSGYTSKRTALLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            23710..24387
                     /locus_tag="VW854_000893"
     CDS             23710..24387
                     /locus_tag="VW854_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV0262156.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAVGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHIIMIPFYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQAFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPGFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            24397..25056
                     /locus_tag="VW854_000894"
     CDS             24397..25056
                     /locus_tag="VW854_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV0262157.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALVEI
                     IRGGLNAVDKGQYEVGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            25305..26495
                     /locus_tag="VW854_000895"
     CDS             25305..26495
                     /locus_tag="VW854_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEV0262158.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKIIHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTNNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(26816..27697)
                     /locus_tag="VW854_000896"
     CDS             complement(26816..27697)
                     /locus_tag="VW854_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEV0262159.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(27838..29166)
                     /gene="vncS"
                     /locus_tag="VW854_000897"
     CDS             complement(27838..29166)
                     /gene="vncS"
                     /locus_tag="VW854_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEV0262160.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENSLTFFIDFPKVVQD"
     gene            complement(29163..29819)
                     /gene="vncR"
                     /locus_tag="VW854_000898"
     CDS             complement(29163..29819)
                     /gene="vncR"
                     /locus_tag="VW854_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEV0262161.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKATKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(29868..29951)
                     /gene="pep27"
                     /locus_tag="VW854_000899"
     CDS             complement(29868..29951)
                     /gene="pep27"
                     /locus_tag="VW854_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEV0262162.1"
                     /translation="MRKEFHNVLSSDQLLTDKRPARDYNRK"
     gene            complement(30131..31510)
                     /gene="vex3"
                     /locus_tag="VW854_000900"
     CDS             complement(30131..31510)
                     /gene="vex3"
                     /locus_tag="VW854_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEV0262163.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(31562..32209)
                     /gene="vex2"
                     /locus_tag="VW854_000901"
     CDS             complement(31562..32209)
                     /gene="vex2"
                     /locus_tag="VW854_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEV0262164.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQIDYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(32222..33499)
                     /locus_tag="VW854_000902"
     CDS             complement(32222..33499)
                     /locus_tag="VW854_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0262165.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKGSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVTLCFLFLFRKSPKEILSSIS"
BASE COUNT         9547 a         7680 c         6202 g        10141 t
ORIGIN      
        1 taaactaacc tcctcattat tagcaaggct tctactgatt ataacctaat tcttagacta
       61 cttcaagact aactgtccaa taataatcta cagataaatc atccactctt aatcaaattt
      121 tattcaatcc atctactcat tcccaaaagg ttgctgtata atgcaagaca tttttcttac
      181 tgcttttact tttccttacc ctcaatttag tgagctaata aattattctt cttcaaaagg
      241 ttgagattta tctagtaact ctaaaacatt aatgataggt agcaacattg cttcactgct
      301 atcaattgcc gaaactgttg aaacaataga acgtaaataa ggataaagaa tagcagtacc
      361 atttacgtat agaatatcgg tgatattttc atctatattt atttcaaaat atcctattac
      421 agttacagat atttttttca tggcatcttt gtcaactaaa gaaacactaa gttttacggc
      481 tccatgacta taatcatctg tatgatcaac cttaactatg ggatataata tatcgaaggt
      541 aaatgtgaac aaactgatta ggaaatttaa accaacttct aacaatctgt tatactcaat
      601 gaaaaccaaa gagcaaacta ggaaactagc ctcaggttgc tcaaaacact gttttgaggt
      661 tgtagatgga actgacgtag tcagctcaaa acactgtttt gaggttgtgg atagaactga
      721 cgaagtcagt aaccatacgc acggcaaggc gacgttgacg tggtttgaag agattttcaa
      781 agagtattag aaagctcgat ggactattcc agattcattc cactagattt cgaaatcgag
      841 taggggataa tctctagccc ctctcacacc accgtacgtg ccgttcggca tacggcggtt
      901 caactaactg ttaacgcatg tcgttcaagg taataatcca aacacgaaac cagtccacgt
      961 ttttcaagga ctgattttga tatagcacgt ttaagtaccg acttttgagc tactaattga
     1021 tagtggtcgc cccagccaga taccttatcc gctatatacg ccttattatc acgttcctgt
     1081 tcgtagagcc acgatttcgc tatcccttcc tctgacgatt cttatttcca gcgtcagact
     1141 taaaacggtc tatccccaga ccgttttaat ccgctacctc acgatagtca gacttgggaa
     1201 tcgctattgg gttcaccggt agcaggtgcc caccgaggat ttacacctca gatatacgac
     1261 atgcccgtcg tacatcaaaa aaagttgaga catagcgtct tcaacttttt tacattactt
     1321 taactactga ttaaagtgag tttacgtcaa ctttgatacc aacaccttga gtagttgtga
     1381 tagtcaagtt tgttacgtaa gttcctttag ctgtagctgg ttttgctttt tggattgttt
     1441 cgttgaaagc tttaaagttt tcaaccaatt tttcagcttc aaatgatact ttaccgatga
     1501 ttgcttgaac gttacctgca cggtcagcac ggtaagtgat tttaccacct ttagactctt
     1561 caactgcttt agcaacatcc attgttacag taccagtttt agggtttggc atcaagttac
     1621 gtggtccaag gacacgtcca agacgtccaa caagagccat catatcaggt gtagcgataa
     1681 ctacgtcgaa gtccaaccaa ccgtcgttga ttttagcaac aaggtcatct tcaccaacaa
     1741 agtctgcacc agcagctttt gcttcttcag cttttgcacc acgtgcgaaa acaagaacac
     1801 gtgaagtttt accagtaccg tttggcaata ccattgctcc acggatttgt tggtcagctt
     1861 ttttaacgtc gatgttcaag ttgtaagcaa cttctacagt tgcatcaaat tttgcaaagt
     1921 tagtttcttt tgcaagtgct acagcttctt ctacactgta tgcttttgtg ctgtcgattt
     1981 tctcaagagc agcacgaagt tgtttgcttt ttttagccat tttctattct ccttgtaagt
     2041 ggttcaatcg attttcatct cccacgtcac ttctcgaaat gatgaagtct tgcgggtttt
     2101 cagtctacta tttttctctt cctacggcct taagctccct tgctgtaccc aagtacaagg
     2161 ctcagtcact tgcctagtat gaaaagcaac taatagactg ctgtgagatt ttctactgtg
     2221 ttattgatta gtcaacaaca gtgaatccca tagaacgagc agtaccttcg atcatacgca
     2281 ttgcagactc tacgtttgct gcgttcaaat ctggcatctt agtttctgca atttcttgta
     2341 cttgtgcacg agtaactgta gcaactttag ttttattagg tgtacctgat cctttttcaa
     2401 cacctgcagc ttttttcaaa agaacagcag ctggtggtgt tttcgtaacg aaagtaaatg
     2461 atttatcttc gtaaactgag ataacaactg gaatgatcat accagcttgg tcagctgtac
     2521 gagcgttgaa ctcttttgtg aatcccatga tgttgatacc agcttgacca agagcaggtc
     2581 caaccggtgg agctggtgta gctttaccag cagggatttg caattttaca agtttttcga
     2641 cttttttagc catttttaaa tcctcctttg tggttttggc ggtaattaga gatttttacc
     2701 tcccacaagt atgtttttca catacccatc cattatacac tatttatctg gaattgcaag
     2761 agaaaacttt catttttaat cgacataatc gccgccttca aagccatagt actcttccaa
     2821 actgagacca cttgcagcaa tttctttggc ttcttttcca acgtagcgga gatgccattc
     2881 ttctgccatg tagccagtct ccttttcctt gcctttaaga tagcgaacaa caaagccata
     2941 gtcagccgca tggtccaaga gccactgggc tgccttttct tcagtaacta aatccccatc
     3001 tgtcccaatc aaatcaaaag caagacccgt ctggtgttca ctgtaacctg ggcgtgctga
     3061 gtagcgatct gctgcttcct taccatcttg attgacatag tcttggtaga gcttagcctg
     3121 agtttcataa ctcctaaacc cactgtattg gtcactgatt ggatagccct cagcctgcat
     3181 agctgcgatt agtttgagca actctgcctt ggctgttgga ttttctcctg gattgtaatc
     3241 ttttgacaaa ggataacgtt tattagctag gataatctca tcgtatttcc cttggatact
     3301 ataatagctc cctttattgc taacttctgc ctttttctga ctagaagctt gagatttgct
     3361 acctacagtt ccttccggtt gactgctttg ctccgttttt tgagtgtttt cttcaacttt
     3421 ggttttttct tgcgaacaag ctgcaagggt caatgctagt aaaccagata aaatgacata
     3481 tctttttttc atttctttct tcctctctct taggccaaat acttctccaa ttcatgagat
     3541 aagattttaa tcatctcttc cagctctggc gattgtactt ggcaatcttc tgccgccatt
     3601 tcttcccagg gcacccacca gactgggcca cggccattca aaccgtgact cttcatatca
     3661 cctgctcttc ggggaaaaag atgccaatga gcgtgggcat cgccatttcc taggagttca
     3721 atattcatct tttcagcctc aaatgctttg gcgactgctt cttggactaa actcatttcc
     3781 tctaaaaagc gaagttttac agaagtttcc atatgatgga gttctgtgac atgatctttt
     3841 gctaaaaata aggtatagcc cttaaagtat tggtagtcac caataacaac atagcctgtt
     3901 tctagttctt ttacaaagta gggattttgc ccagctttaa tgagttcgat tctctgacag
     3961 attaagcaca ttctatgata ccgcctcgct cttaagataa gcatcaacca tacgctcagt
     4021 cgctactact gaatcgatgt gggtgcgctc ataagagtga ctggactcaa taccagctcc
     4081 aagaagtgca tgcttgacct ctgcacctgc tgacatcgct gctgaagcgt ccgatccgta
     4141 aaatggatag atgtcgagct taaatggaat atcttgctct ttagccaagg caaccaagtg
     4201 ttgacggaag tcatagtgat aaggacctga agcatccttg acacagatag atactgtgta
     4261 ctcatctgtc tgctggtcat ctcccatagc tcccatatcc acagccagat attctactac
     4321 ctgagcagga atgttagaat tagcaccatg tcctacttct tcaaaaactg aaaaagcaaa
     4381 atgagttgtt actggcaatt caatcttctc tttcttataa atacgaagca ggttgagcaa
     4441 aatcgctgcg ctgaccttat catccaaatg acgagacttg ataaaacctg tatcagtcac
     4501 aacagttcgt ggatcgaagc tgataaaatc accgacctca atcccaagag cacgagtttc
     4561 tttttcattg cttactttta catccaaacg cacttccata ttgtcctgcg tgcgttctgc
     4621 agttcctgca tccttgtaga catggcaaga agtttggtgg atgaggatgg ttcctgatac
     4681 cttttgacct gtgctagcca catgaacggt acagttttca ccttcaatca tgttccaagg
     4741 aaaaccaccg atacggtcca atttaagacg accatctggt ttgacagcac gaacaatagc
     4801 acccagcgtg tccacatggg cagtcacata gcgatgttgc tcatcatttt gacctttgat
     4861 ggtcacattg acaccaccct tggctgtgcg aactggctgg taaccaaaac cttctagagt
     4921 cttgactaaa taattcgaaa tttcacgagt gaagcccgtt ggcgatgcga tggctgtcag
     4981 ttctttgata tattctactg tttgattcat ttcttcacct ctttcgctac ctattataac
     5041 acatttatca tcggataaaa aaagaaaatc actcctgtag acttggctac aaaagtgatt
     5101 taagtgatta ttccatttca aaaacatcgc tttcttgctt gtttggtaga accaatgaga
     5161 gcaagattcc aacaatggct ggcaccaacc atgggagaga tgccttggca aaaggaagaa
     5221 cgctaacaag atttgcaaga aactcaacct taaacgagct tcctagtacg cttgcaatgg
     5281 caataactgt aaccacagca attgtcaact gcatacctgg ttttgaaagg gcaacaaatt
     5341 tgttgacaat gacaatcata acgatagcaa tcgtgattgg gtacaagata accagtactg
     5401 gaattgagta cttgataatc gcatcaagac ccaaattggc aatagcaaat ccaatcaagg
     5461 taaaggctgt cgcataaacc ttgtagctga tttgtgggaa gcgctcatta aagaactcag
     5521 ctgttgacac aatcaaacca acagtcgttg tgaagcaggt tacggtaacc atagctgcaa
     5581 ggaagagttg agctgttgag ccaaagattt cttgagtggc ttgtgacaag atgtaaacac
     5641 ctggtgttcc acccttcatc gcttcagctg gtactgggaa atgatttcca agaaaaccta
     5701 aaccgatgta aagagcgctg aaggcaaggg caacaacgat accaacaacc caaatagttg
     5761 aaatgtattc tttcttactt gaaaatccaa gttgtttcaa ggtttgaact gcgattacgc
     5821 taaaggccac tgaagcaagg gcgtccaagg tattgtaacc ttctaggaaa cctgtaccaa
     5881 aggcagaagc ttgataagca gctgaagcag cttgaggact tgttccacca tatttgatag
     5941 ctcccagaac gaccaagata acaatcaaaa ttgcaaagac tggcgttaaa atacgtccaa
     6001 tgcggtctaa gatttttgat ggattaagcg aaatcaaata ggctgccgca aaatacagaa
     6061 ccgtaaatac aatcaagcca agtcctttat ttgcatccga caaaaggggg ctaatcccta
     6121 cttcgtaagc tgttgtagca gtacgtgggg tagcaaagaa tggaccgatt gacaagtaaa
     6181 gaactgagag gtaaagagtc gcaaaccaag gcgctatctt cgttgaaatc tcgtagatat
     6241 atcctttagg atttagcgtt ccaataataa gggtcaagac ggcgatacca acgcctgaaa
     6301 agacaaaacc tgcgatggca ggaagaaaat gttctccaga tagagcacct agagaaggcg
     6361 gaaaaatcaa gttccccgca ccaaaaaata ttccaaacag gagcaaacct gttagggtac
     6421 cttttttagc cataaaaatc tccttcatat ttataaaata ctgacctagt ataccacgat
     6481 taggagtctg aaaaaagcat cacgaggcaa atattaaatg ttttcaattt tcttaaaaat
     6541 aagaattatc taaaaataaa actctcctac tcagtccaca ccaagtagga gaaatgcaaa
     6601 attttaaagt tcttcaaagg ctaacatgcc accctggaca ttgataacat tataaccttg
     6661 ttctaatagg aattggcaag cacgcgccga tctcattcca gatttgcaaa taatataatg
     6721 caagcgatct ttgtccaatt aatcataact atcagccaat tgactaagcg gtaggttgtg
     6781 ggcaccttct aaatgaagag ctgcaaactc atccacttct ctcacatcca ctaaagaaag
     6841 ctggtcgttt tggtaaagct ggtaaaatgc gtcaaaggct atttctttca ttctttttct
     6901 tctaatactc ttcgaaaatc tcttcaaacc acgtcagcgt cgccttaccg tgcgtatatg
     6961 taactgactt cgtcagtttt atctgcaacc tcaaagcagt gctttgagct gacttcgtca
     7021 gttccatcca caacctcaaa gctgtacttt gagcagccta cgactagctt cctagtttgc
     7081 tctttgattt tcattgacta taaaatggtt ttaattcttt ttttcaaatc tggcactact
     7141 tctgcctcaa accaaggatt tttgaccatc cagatttgat ttcgtggtga tgggtgaact
     7201 agcggaaaat aggctggcag atagtcttta tagtgtttca ccctctccgt taccttccca
     7261 ctgattttct cctgtaaata gtaggcttgg gcatattgcc caatcaagag ggttaactga
     7321 atatcaggca attcctgtaa gagctgcgga tgccattttt ctgcaaaacc tgtacgaggc
     7381 ggaagatcac ccgacttgcc atgtcctgga aagtagaaat ccataggcaa aacagcaaaa
     7441 taacctgaat tgtaaaaggt atcttcatcc acacctagcc agtcccgcaa gcggtcacca
     7501 cttttatctt tccagtaaag gcctgcttct tgagttttaa gtcccggagc ctgaccgatg
     7561 atattgatgc gagcagtttt tggcgctgca aagagaggct caatgccacg ctctgtatag
     7621 ctggcattct gtgaatccgc catgatagcc tgtttgattc tttcgatttg agacatctac
     7681 tttccctcca aaaagaagtc caagcataag atctcagact tctatcgttt tatttgatca
     7741 attcgtaaat agcttcggca taaattgctg ctgctcggaa aagatcatcc aaggcgataa
     7801 attcattggc ttggtgcatg gtatcaatcg agtctgggaa catagcaccg taggcaactc
     7861 cgcgttctag caagcgacca aaggttccac caccgatgac ttgttcatga cctttaaggc
     7921 cagtttgttt ttcatagaca ttcaacaagg tttgcacaag tggatcttcc attggcacat
     7981 agtgaggcgt gtgaccgtgt tcagacaggc taacagaaac aactggcaag ttttcaagga
     8041 ttgacttgat ttgttctgga cttgttcctt ttggatagcg gatgttgagg gcaatggtat
     8101 tatcagcact tgtttcatcg aagcggaaga cgccggcatt catagaaaga gcacccatct
     8161 tttcatccac atgagcaatc ttaagatttt caccctcatg atcgttcaag agaattttac
     8221 ctgcgatgtc aaggtaatct ttggctggac cagcaaagcc aaactggctg aggaagaggg
     8281 caaggtaagt tgcgccattg acacctgaag caggcatagc accgtgggct gatttaccaa
     8341 tgatcgtcac cttgtatttg ccagcttctt cttggagttc tcctctaagt ttgtgttctg
     8401 caacaaaggc atctagttta gcttgcaagt cagccaaatc acctgaaacg actgctgttg
     8461 ctgattctgg taccatattt tcacgtaaac cacctgtaaa gctgtgaaga cgggcagcac
     8521 ctgtattttc tcctgcaaag tggaggtatt ccgtgatatt tcctttttca ccattgatga
     8581 ttggaaattc agcatctggt gagaaaccga aatctggttt ggcaagtcct acgtgctcaa
     8641 agtagtagtc catgtctgcc cagcctgatt cttcgtctgt tccaacgatg aagcgaattt
     8701 tcttagaagt tggaagaccc aattctttga tgattttcaa accatagtaa caagctgttg
     8761 taggaccctt atcgtccgaa gccccgcgcg cataaaggcg accatctttg atagttggtg
     8821 tgtaaggaac tgtatcccaa ccgctaccag ctggaaccac gtccatatgg gcaaagattc
     8881 cgagaacttc ttctccatca ccaaactcaa aatgtcctgc gtagttatcg acattcttag
     8941 ttgggtagcc atcgcggtct gcgatttcaa ggaatttctc caaggctttt actggaccag
     9001 gtccaaatgg atgctgggca tcagccttgc tatcatcacg ttctgagttg atttccaaaa
     9061 ggctaaacaa gtcagccaag aggtcttctt tgcgtttttc tacttctgct gtaaaatcaa
     9121 ttgctgtcat tttttcttct ttctatcttt tctcgatgat ttcatctact ggcaagcggt
     9181 agcttggttc caatttttcg tctgtataac ccactgtgat caagagttct gggcggaaac
     9241 ggtcttcgat ttccaaaact tcattaactt ttgatttgtc aaaaccaaga ataatgttag
     9301 aaccaattcc ttggtctgtc aatgcaagaa ccaagttcat ggcaaccaaa cctgcattga
     9361 gagctaggta gtcgctgact tgttgctcat tgtaacgggc aaactcagct ggcaaatttt
     9421 tcatgaagta ttgaagttgt tcttctgaga aattgttagc accaccaact cgggcaatct
     9481 tacgagcgcg tttggcaagg tctgtatctg taaatagggc aatggttaca ggtgctgatg
     9541 atacctgctc aaagtttgaa ccataagcca actttgccag ttcagcattt ttctcacgaa
     9601 caactacaaa tttccaaggc tggctgttgt gggcgcttgg cgccaaggtt gcaatttcga
     9661 tagccgtacg cacatctttt ggatcaacag gcttatcagt aaaatgctta gtcgcatgac
     9721 gttttttatt taattcaaga aatttcataa tcgatttcct tttctaattc tactgcttcc
     9781 attctaccat aatttgaaag agcttgcagg gatttttcat gattaagttt ttctatcaca
     9841 gacataaaag atatatattg gtttatcagg aaagtttctg ataaactagc aagtacttta
     9901 catttgaatg gagacagtat gaaaaaattt agcctattac tagctatcct accatttttg
     9961 gttgcctgtg ggaatcaagc tacacccaaa gagactagcg ctcaaaagac aatcgtcctt
    10021 gctacagctg gcgacgtgcc accatttgac tacgaagata aaggcaatct gacaggcttc
    10081 gatatcgaag ttctaaaggc agtagatgaa aaactcagcg actacgagat tcaatttcaa
    10141 agaaccgcct gggaaagtat cttcccagga cttgattctg gtcactatca ggctgcggcc
    10201 aataacttga gttacacaaa agagcgcgct gaaaaatacc tctactcact tccgatttcc
    10261 aacaatcccc tcgttctcgt cagcaacaag aaaaatcctt tgacttctct tgaccagatc
    10321 gctggtaaaa caacacaaga ggataccgga acttctaacg ctcaattcat caatcactgg
    10381 aatcagaaac acactgataa tcccgctaca attgattttt ctggtgagga tattggtaaa
    10441 cgaatcctag accttgctaa cggagagttt gatttcctag tttttgacaa ggtatccgtt
    10501 caaaagatta tcaaggaccg tggtttagac ctctcagtcg ttgatttacc ttctgcagat
    10561 agccccagca attatatcat tttctcaagc gaccaaaaag agtttaaaga gaaatttgat
    10621 aaagcgctca aagaactcta tcaagacgga acccttgaaa aactcagcaa tacctatcta
    10681 ggtggttctt acctcccaga tcaatctcag ttacaataat actcttcgaa aatctcttca
    10741 aaccacgtca gctttatctg caacctcaaa gctgtgcttt gagcaaccta cggctagctt
    10801 cctagtttgc tctttgattt tcattgagta taagatatat aaaagcgatt ggactaccaa
    10861 tcgtttttag tttgcattgg ttattttagg aaactcttac aaaaaatcaa aaaaattctt
    10921 cacatcctct acatacccat gcttttctat taagcttgct aataattcca gattgtgccc
    10981 ctgatagtta tcgtcacgac gcagttcttc atacatttcg ataaagggaa gtcccttgga
    11041 cttggccaat tctaactcag cctcgtaatc ataagcaact ttacgaaata ggatatttac
    11101 caattcccca tcttcaacct ctatcacggc atactgggca cggtgatttt ttaacgcctc
    11161 ccaattaaaa tagggcatgc caatcgaccc tggattgatg atttgttgcc cttgactacc
    11221 ataacgaagc aactgcttgt gaacatgacc ataaactgcc acgtccgttt ccgcatctag
    11281 cagttggtca aatttctctg tatcattctc aactagcaag tcaccaccat agtttttgtc
    11341 aggtaaatta tgagagatag aaaagcgcaa tccgtcaatt tctttctttt ccagcaaagg
    11401 caagcttcgt agccagacaa tcgttgcagg atccattcgc tccatcaaaa actgggtcat
    11461 gcgcattgac tggatttctt tcggatattc caaaccatat tcgccatcca agacctcaag
    11521 gacacgatca tcccaattgc ctcgaacact tgctgtgata ggaaggtcct ttagcagggc
    11581 gactaagtca tttgcgcctg gaccaggaag aaaaatatct cccagaagcc aatattcact
    11641 ggccccttga tttttagcat ctgcaatcac tgcttctagc gccgtcgcat tgccatgaac
    11701 atctgataaa attgcgattt tatggttcat tgtggtttcc ttccgtttgg taatctactc
    11761 tttcttctgc tacttgaggc aaggcttctc cctgcgggtt caactttctt tgcacagcct
    11821 ctgcaacgac tctaggtacc ccaacttcga caatctcatc cacactggct tccttgattt
    11881 tggtcaaaga cttgaaatgc ttcataagat tctgcttgcg tttaggtccc agaccgtcaa
    11941 tcccatccaa ttgagatgag aaagaatttt tggagcgcag ttggcggtgg aaagtgatag
    12001 caaagcggtg cacctcatct tggatgcgtt ggaggaggaa aaattcctga gaattgcgag
    12061 acaagtccac cacctcaagc ggatctccaa agagcaattc atgggtttgg tgcttatcat
    12121 tcttttgcag cccagcaatt ggaatatcca agcccagttc ctcttggata acctgcttag
    12181 cgatattgac ttgaccttgc cccccatcaa tcacaatcaa atctggagga gtcaaagcct
    12241 cacgctgtac tcgaccatag cgtctgcgaa tgacctctct catgctggca tagtcgtctg
    12301 gtccaacaac cgtttttatc ttgtacttac ggtaatcctt cttactcggt ttaccgttga
    12361 caaagaccac catagccgaa acagggctag ttcccatgat attagagtta tcgaaggact
    12421 cgatacgtac tggggtcggg atttggagca aacgccctag attttcaata gctccttgag
    12481 tcttttcgac agatttttct agcaggttaa atttctgctc cagactaaca cgtgcatttt
    12541 ttatggctag attgaccagt tgttttttct ctccacgttg aggcttaaga atcttggaat
    12601 ccaccaaggc cttgacagcc tcttcgtcaa tatcctgcgg aatcagtacc tcattgggaa
    12661 ctagatgaga tttttcttga tagaattgtc ctacataggt caaaaaatcc tcatctggat
    12721 cattgaagta ggggaagaga ttgacatcgc gctcgataag cttaccctga cggacaaaga
    12781 aaacctgcac acacatccag cccttatcca catagtagcc aaagacatcg cgattttgca
    12841 aatctttcgc catgacccgt tgcttggttc gaagcgttcc aatagcctga atcaggtcac
    12901 ggtattccgc cgcacgttca aactccatac tttgtgctgc tactgccatt ttactcttga
    12961 gatcatcgat gattttgtta tcctgccctt tcagaaaatc agaaacctcc tgggccatag
    13021 acttgaaata agtctcatcc ttcttacaga tggtgtgggc catgcattga ccgatatggt
    13081 aataaaaaca gaccttagag ggcgggttgg tacacttacg aaaagggaat atccgatcca
    13141 gcaaccgctt gatttcattg gctgccccca catcgggata gggtccaaaa taaagacctc
    13201 cgtccttttt gacctgacga gtgataatca agcgtggata gcgctcattg gtgattttga
    13261 tgaaaggata ggacttgtca tccttgagca tgatattgta cttgggcttg ttttccttga
    13321 tcaggttgat ttctaggaga agtgcctcaa tattagactc cgtaacaata aattcaaaat
    13381 ccacaatttc agacaccaga gcctctgtct tggtatcatg acttccacga aaataggacc
    13441 gtactcgatt acgcagattt ttagcctttc ctacatagat aatggtgcca tttttatcct
    13501 tatgaatgta gcaaccaggg ctggtcggca agagctctag ttttgattta atcaagttgt
    13561 tcatagttcc attatagcaa aaaaagtagg gaaagatagt accctactca ctagtctcat
    13621 atatttttcg gaggtttgca acatcctctt cctggatacc ataaaaggaa cctgctggtt
    13681 gcatgacaga cttctcatct gtatggtcca agccaatcgc atgacctaac tcatgttctg
    13741 ccgtatggac aaggcgttca taggagtagc catagtatgg attggacaaa taataatgat
    13801 tcaaccgcac cgttacggac aagaattgcc ctgttaagag attagtttga ctttccgcct
    13861 ctcctgccac aggagtgcct ccgtcgttca tctccgtagc cgtaatatcc gccttactag
    13921 actcagtcac gagttcaaag ttaaaagcac cagtttgatt ccagttctgg attgcttcta
    13981 aataagcctc ttgaaagcgt gaatccatct gcggatccag gtaaatacga gcagaggcct
    14041 gtgcccacct tccttcagaa tgctggtgac tatctgtctg gcttaactta ggcagttgcc
    14101 ctgttttctc ccattggtca atactttgtt gaccaatttt tactgaccgt tctgcttgct
    14161 ttagcgctcc ttgaaaatcc ccgttcagat accagagaag tccgaaagca agcacacata
    14221 agagcacaac tatccaaacc agacgccaaa acaaacgcca cacaaaaaaa aagaaagctc
    14281 ctatcaaacg aaaaagccag cgcatgtctc tccacctttc tagcaaataa gaattatttt
    14341 actaaaagaa gctaaaagaa atctaaatac tgactttttc atcttaacgt cctactattt
    14401 ttttgagtaa ctggatagct ttgtatttta aaggcgatgg atggtaacgg aaagtacccg
    14461 ctttgcgtac aatatagcca ttaaaattct gtttaaaacg caaaacacca tcacttccat
    14521 caaaaatccc ttgaatgcct aggaagttgt atttaggtat tccacgtttt atgctttcca
    14581 acataacata tttttgaagc agggcagggg catagaactt attaaactca gtgtaggaac
    14641 cactaaagag ataagtcgtt tcctgaggca tataaacaaa taaactacca gctaaaacaa
    14701 tatcttcttc tccatatttt tcaatcaagt ctcgcgcttc tgcttttcga acttcaaacg
    14761 tttcaaattg actagaatat tctctcagtt gattttgttt tttctcagaa tgaggatttt
    14821 tactcaaatc aagtcgcaac ttgtccaagt tttcttctag tttactttgt tcaccttgca
    14881 atttgctcat atagtccgaa aaatttaagc ttgctatgag aaactccgct tgttctccaa
    14941 aagtatcata aaaatgctca taatattcta aacttttatc actatattct ctacgttcag
    15001 aggtttcttt tgttatattc ttaaaaatcg atagttcttc acgttttaac tttttcaacc
    15061 gaatgccaaa ggtttcagcc tttttcacca agggtttacc ctttttgcta aaacttttaa
    15121 gcaaactctt ttcagttaat tcagttaaat ctttatagta taaccaatct ggttctccac
    15181 ctgggtaacc tattgttaag ccatcaaatt gataacctaa atcagtcaaa ccttgaataa
    15241 tacttttttt ctcagcatct attggattac cttggctatc aaaagtttga taagtttcat
    15301 aaggttttac aagcaactct aatacaccat tttgcttggc atattctttt aactctgcat
    15361 aaaaaactgg aagagcatct tgttgggtat aaatcggccc cgaattgagt tccatatgca
    15421 gaccacccag catgggcaag ctataaacca gagctgcaac ttgaatttct ccttcttgtt
    15481 tcaaagcaag ataaacaatt cgagcccctc ttttttctag caaatccccc atctggacag
    15541 attgcataaa ggaacgagaa gaaacctgat cagaataagt ctgaaactct tctttcgtga
    15601 gtgttgttag tgccatatac ttactttcta tgtttttttc ttaatgtttt acggaaatca
    15661 agagcaagtc ttaacagagg atagagagga tgagtgggca ttgtaaattc acccaagtat
    15721 tcttcaatcg ttggattaaa tttttcctta aaatgataaa gtccaccatt gagagagttt
    15781 tcaacaccac ctaaattttg ccagaccata cctcgctcaa aggcatagcg agccgtttca
    15841 taccatgtta aaattggtgc attgtaacgt ttaaaatcat catccatacc agcatatata
    15901 ttgacagagg tagtaccaaa ttccaaactc aaagtagccg ctaaaggaac tctcgcttga
    15961 cctacatcta tatattcctg caagaaggtc aattcctcta acaaacgttc tttttccttc
    16021 ttctgcgctt ctacttttga agttcgagtc gactcagtaa acgtctcttc caaggctcta
    16081 tttttcgcta actgttcttc taactcttgc gaacgtttag aaacatccaa ggtggccaag
    16141 gtgatatagg ccttgtcctt aaaattatct aacaattttt tataataggc ttcattcctc
    16201 aaatgaatct cttttcgctt ctcagttttt ttcatcaact ccgaaaatga atctaatagt
    16261 tccagtccac catattgaat ctcaagccct ttgtttcgtg ctgttcgaat agcctgtttt
    16321 gttgacttgg aaagtttatc ttcttcaaaa ttttccttgt atattttcgc ctgaatacga
    16381 ggttgaatgg tgtctcccat ttcctccgtt tttcctgacc accttactcc catttgttgc
    16441 aaactatcaa taatagccag attttcagga aattctgtct tttcctgatt gattaaactt
    16501 tgagataggc aaatacttgg gtcaaaagtc acaaaaaccg ctctcttact gcgagcatag
    16561 gacttaatag actgaatggc aaaattcaag agttctttat ccccataatc caatataggt
    16621 cctcttggga tgtaaaacat tttatagcct agcggaagag ttctaatcaa aatactagct
    16681 gtcgccagta atttttcttc cctgtaaaca ccaaacttct catgttgcca attagactta
    16741 acttcctccc aagcactact ttgtaataca ttggctaatt catgttcttt gacaaactga
    16801 tcatattcta atgtgggaat gccaatttga taacggtaca tttcctactc tctttcctcc
    16861 agtattttat tactattcta ctactttcgt ttgaaaagtc tagtgaaaac atattttatt
    16921 ttagttctac tccccctatg ctgaagttca aactaagtca gtctctcttc taatttgaaa
    16981 tcaattggta gggttgttaa aaaaacttcc aattgttttt cccagtagta ccactcgtga
    17041 gttccagcgc tatggctata ggtcacatca aaacctagtt ttttgagatt tttcactacg
    17101 agattattgg cttcgtacaa gaaatcctgt tcgccacacc acgcccaaag tttggtcttt
    17161 ttatccgatt ttttagccag actttcaaga gaatagggac tagttgtcca gtctttaatc
    17221 tctccaaaaa cacctctcca gtaggctgga cttcccagat tttgactctc aggagaaaaa
    17281 tcttgaaagc tcaatgcacc tgaaaagcta gctgcatgag aaaaacgatt tgtcgcaaga
    17341 gccagtttga agcagccgta gcctcccata gaaagaccag cgataaaggt cttttcacac
    17401 ttgctagtca tattagggaa gaagcgtttc agaacctgtg gcaattcctc tgctaaagcc
    17461 gtgtagtagt caaaaccata ctgggtatcg gtgtaccaac cattgctggt attgggcata
    17521 acaacgatga gattagttcc tcgaagcaag cgttctacat tggtccgctt aagccaacta
    17581 ttatgatttc cagacatccc gtgtaaaagg tacaagacgg gaatatcttc acactctggt
    17641 tcttccactc gattggcatc agggtagagg acattcaccc cccactccat atccaatact
    17701 tgtgagtaat actcgatttt cattactgcc atgattttct ccttctattt ttctataaga
    17761 aaaagccgaa actcgacttt ctctctgttt atttcaacga ttattttgct tccatgacta
    17821 ctggtaaaat agctggacga cgcttggttt ggtcaaagag atacttggtc aaattatctc
    17881 gaaccttccc tttaagatct gcccagtcaa agtcatctcc ttgaagatac tcttctaccg
    17941 tttggttaat caattctgaa ctttcacgga gaatatcgcg actcttcttg agataaacaa
    18001 atccacgcgt gtgaacacga gccctagcca caattttctt ctcacgacgg ttgactgtaa
    18061 tagccacgat gaaaattcca tcctctgaca agaccttacg gtcacgaaga acaacatttc
    18121 caacatcacc aatggcattc ccatcaatca agatatctcc tgctgaaacc gatccagctg
    18181 gaacaaagtc tccattctcg taagccatgg tcgtcccctt tttaggaatg aagatgcgtt
    18241 ctggcaacat cccaactgcc atggcagcct tagcgtgagc atccaactca cgatactccc
    18301 cttggacagg gaagaggtac ttaggttgca aaagattgat catcagctgc aaatcacgca
    18361 catttccgtg ccctgataca tgtaaacttt gggtaatcaa tttgacaacc ccacctgcct
    18421 gataaatcat attttccaca cgcgcaacaa aggcttcttt agcaatagac ggagccgtag
    18481 caatatagac taggtcccca tccttgattt ctacataacg atggcgacca atcgacatct
    18541 tacgaagtcc attgataggc tcacccatac gacctgtctc aagaataatc aactcatggt
    18601 cttcaaagcg agacatatct ttaggcttaa tcaaaagaat ttcgttggct aaagacaact
    18661 tcttaagacg aatcgctgtg cggacgatat tttcaatatc aaatcctgtc aagacgatac
    18721 gtcgacctgt tttatccgca gcgtcaaaaa tctgctgaat acgagaaaga ttactggaaa
    18781 cagctgcaac gatgatacga ccttcccagt cagcaatagt ttgggtaatt tcatccctaa
    18841 cttcactttc actagccacc tgaatattgc tgtctgcatt ggccgaatca ctgaggagag
    18901 ccaggacgcc gtcacgacca atctctgcca aacgagcaaa atcagttgca taagattcac
    18961 tagccgtttg gtcaaatttg aagtcacctg tataaacgat gcttccttcc gatgtcttca
    19021 agacaattcc cagactctct ggaacggagt aagtcgtagg gaagaaggaa accactgtcc
    19081 caccaaaatc aatctccgta ttctcatcaa tgacatggaa atcattaaat ttcttaacgg
    19141 catcatttcc tttgacaaag agctttgcca actcaatggt caactcagac ccaaatacag
    19201 gaactttagc ctctgccaag agatacggta gagcaccaat ggcatccgca tgcccgtggg
    19261 tcaagaaaac cccagcaata cggtcgctat tttcaaaaag gtaatccatg tttggaatca
    19321 ccacatcgac ccctaattgt tcattttcag gatattttaa ccctacattc aaaacaaaaa
    19381 tggactctcc aatttcagca atgtacatat tttttccatt ctcacgcaca ccaccaagtg
    19441 ttgttaaact gatattactc atttttcctc caaaatctta gtttatcttg attatagggc
    19501 aattctaccc tcttattcta aaagttctga tactttcagc cgaatctttt cctaccatta
    19561 taccattttt ttgatgattt tcaaaatgaa ggtctgtttt caaaaaagag ttgatttccc
    19621 aactctttcc taacttctac tctttttcca tcaaaaaatc ataaatttct tgcaccgtac
    19681 ccagcgccat catttcttgg tctttaacgg tttgtttgag attttggtaa atttgacttt
    19741 ctcctatctc ccgctttggc gcctctttat tgactgtcat cacaccatct tttatcgtca
    19801 caaagcctag ttcttcaaat acttgaatca tcttgaccag caagatttgt tgaatattaa
    19861 gatatgcagc caaatctttc agcttgtagc gaatatcaaa ctctgggaac tggtaaatag
    19921 tcttgtacaa tttggcaaac tgatctctag tcccataacc tgtcagataa taagccttgt
    19981 caatatcatt tttgaaatag acagcagaga aatgctgttc ctgaaaaatg gtcttcagct
    20041 gagtaatatc ctctggaatg ttttttacga caacagcttc actagccgca agatttggca
    20101 gttctccagg taaatccaag actggaacac cttctggcaa gactgcattt tttccacgaa
    20161 tgttaaaaag ttgaacacct tccactcgcg catccaccat catcaactgg agggcagttt
    20221 ggccattcca ttggttgaca gacaatttaa ccgctaactc tagattcttg gtttgagaaa
    20281 actctgtcgc ccatctgcct tgaccaaagg ctaccacttc aaaactcgcc tcacccttgg
    20341 aaattttcag ctttagatgg gcattacctg cccccatagt acgagcactt tcgacctgaa
    20401 aattcttgat ataaaaaata ggtttctgat tatccattcc aaaaggagct aaacgttcaa
    20461 aacttttgac cgtttccaag ctaagtgcct ccaaatccaa ctcttcatct aggtttaact
    20521 tattcttgcc accagcatct gcaccttttt cacgaacata atcttccaaa acctgagata
    20581 aatctgagag ttgctcaact tccagcgtca tacccgctgc acctgcatga cctccaaagg
    20641 tgatgaagag gtctcgatgg ggatccagag cttcaaaaat atcgaccgct tccacactac
    20701 gagcactgcc cttggcacga ccgtcttcta tattaagaac aatgactgtc tgtcccaatt
    20761 cttccaataa acgaccagcc acgattccta gaaccccagg attccagcct tccttggcca
    20821 agacctgaac cttcttctca ggatccacca tggtcttggc ttcttcatag atagactgaa
    20881 cgatttcctt gcgctcttcg tttttctggt gaatcataag ggcaatctca tgcgcttctt
    20941 catcatcaaa tccagtcaac aaatcaatgg caggattggg atcatccaag cgacccaagg
    21001 cattcaaacg aggagcaatc tggaaaccaa ccgtttcttc tgttacttcg ttggcagcaa
    21061 tcccagccat gtccagcatt tcttgcagac caatgcgctg ggtatgaccc aacatttcca
    21121 gaccatattg aactaagata cgattttcat ccgtcagact caccatatct gcaatagttc
    21181 caatagcgac caaatcaagc aattccactt gcacttcttc taacagggca caagccaact
    21241 tgaaagcaac tccacaacca gccaaatatt taaaaggata atccgcatct ggatgttcag
    21301 gatggacaat agcataagca tctggcaggg tttcaggcat ggaatgatgg tctgtcacaa
    21361 tgacatctac tcccatagac tgagccaatg caatagcctc atgaccagca accccattgt
    21421 ccaccgtcac aatcaaggaa atcccttctt gctcgataaa gtatttataa acactagcat
    21481 tagggccata gccatcggta aaacgatttg gcaggtaaac tcggcactca gcaccaagtt
    21541 gttccaaact ttccttcaca atagaagccg aagtcatgcc atccgcatca tagtctccat
    21601 aaacaagaat attttcccct tcttcaatag cctgacgaat ccgctccact gccttgtcca
    21661 tatcatggag cagataagca tcatgtaagt cctccaagga aggttctaaa aacttcttca
    21721 gactttcttg gttctgaatc cctctctcaa acaataaccg agccacctca ggacccagtc
    21781 cagccttctt ggctatcttt gtaaaatccg catcttctac ctgcagggca aactgccatt
    21841 cataagtagg tgttatcaaa aagacatcca ctctttctaa gaattctatt gcttcattat
    21901 aacatgattt agacgttttc tctatccaga ttgctttttt ataacatttt atagtatatt
    21961 gaaactagaa tagtacacct ctacttctaa aatattgtta gaaatcgatt tgactgtccc
    22021 gatcgatttt tcctgttctt atttcactat agtgaatttt ttcagatatg atttgacaag
    22081 aacattcttt tattgtagaa ccgtgttata aaatataaca caatagagat ctctcatggc
    22141 tttagtagaa tttaaaaacg tcgaaaaata ttacggagac taccacgcat tccgcaacat
    22201 caatctccgt tttgaaaaag gacaagttgt tgtcctgctt ggaccttctg gctctgggaa
    22261 gtccactctt atccgtacga tcaatggttt agagactgtt gacaaaggaa gtctcctagt
    22321 caatgggcac caagttgctg gtgccagcca gaaagatttg gtacctcttc gcaaggaagt
    22381 cggcatggtt tttcaacatt ttaaccttta tccacacaaa gctgtgttag aaaacgtaac
    22441 gcttgcaccc attgaagttc taggaattga taaaaaagaa gctgaaaaaa ccgcccaaaa
    22501 atatctggaa tttgtaaata tgtgggacaa gaaagattcc tatcccgcca tgctatctgg
    22561 tggacaaaaa cagcggatcg ccatcgctcg tggtcttgct atgcatccgg aactcctcct
    22621 ctttgatgaa ccaacatctg ctcttgatcc tgagactatc ggagatgttc tagcagttat
    22681 gcagaaactg gcgcatgatg ggatgaacat gatcatcgtt acccacgaaa tgggctttgc
    22741 tcgagaggtt gcggaccgca ttatctttat ggccgacgga gaagttttag tagatacgac
    22801 agatgtcgat aacttttttg acaatccaag cgaacctcgt gcccaacaat tcctcagcaa
    22861 aattatcaac cacgaaagtg acaaagtcaa ataaggaggc gcctatgaaa aaaattcttt
    22921 ttatcagcat tattgattag ccttttcggc cttgctgctg ccaaaccagt ccaggctgat
    22981 acaagtatcg cagacattca aaaaagaggc gaactggttg tcggtgtcaa acaagacgtt
    23041 cccaattttg gttacaaaga tcccaagacc ggtacttatt ctggtatcga aaccgacttg
    23101 gccaagatgg tagctgatga actcaaggtc aagattcgct atgtgccggt tacagcacaa
    23161 acccgcggcc cccttctaga caatgaacag gtcgatatgg atatcgcgac ctttaccatc
    23221 acggacgaac gcaaaaaact ctacaacttt accagtccct actacacaga cgcttctgga
    23281 tttttggtca ataaatctgc caaaatcaaa aagattgagg acctaaacgg caaaaccatc
    23341 ggagtcgccc aaggttctat cacccaacgc ctgattactg aactgggtaa aaagaaaggt
    23401 ctgaagttta aattcgtcga acttggttcc tacccagaat tgattacttc cctgcacgct
    23461 catcgtatcg ataccttttc cgttgaccgc tctattctat ctggctacac tagtaaacgg
    23521 acagcactac tagatgatag tttcaagcca tctgactacg gtattgttac caagaaatca
    23581 aatacagagc tcaacgacta tcttgataac ttggttacta aatggagcaa ggatggtagt
    23641 ttgcagaaac tttatgaccg ttacaagctc aaaccatcta gccatactgc agattaagga
    23701 ggacacccca tgacagattt atcatcttgg acagcctatt ttcaggattt tggacaattt
    23761 ttcaatggtt tcctcttcac ccttgcccta gcggttggtt cctttatcct cgctatggtt
    23821 ttgggcatct tctttggtgc catgtcaacc agtaaacgtc caattttacg cattttagct
    23881 cgcatctttg ttgaatttta ccaaaatact cccctcttgg tgcagtttgt tattgttttt
    23941 tatggcctac ctcttatcag tgaccatatc atcatgattc cattttattg gacagccgtt
    24001 ctctgcgtgg gactctatca cggcgcttat atcgctgagg ttattcgttc agggattcag
    24061 tctattccta gtggtcagat ggaggccgcc ttgtcgcaag gttttaccta tatcagtgcc
    24121 atgcgcttga ttatcttgcc tcaggccttc cgcatcattc tccctccatt gaccaaccaa
    24181 attgttaacc tcatcaagaa cacctctact gtagctatca tctctggagt agacttgatg
    24241 tttgtgacta agtcttggtc ggctctcaac ggaaactata tcccaggctt tttaggtgct
    24301 gctcttctct actttgccct atgcttccct gttgcccagt ttggtcgcaa gatggagcaa
    24361 gccaacaaaa aagcctattc actttaggag gttactatgg aatctatttt agaagtttta
    24421 accccagata acctagtctt tatctttaaa ggatttggct tgaccctcta tatttctctg
    24481 attgccatca tcctctctac tatcatcggt acggtgctag ctgtcatgag aaatggcaaa
    24541 aatcctgtct tgcgaatcat ctccagtatt tatatcgagt ttgtgcgcaa cgttcccaac
    24601 cttctctgga tttttactat ctttttggtg ttcaaaatga aatccacacc agcaggtatt
    24661 acagccttta ctctctttac atcagcagcc ttggttgaga ttattcgagg cggtctcaat
    24721 gccgtagaca agggacaata cgaagtagga atgtcacaag gcttcacctc agcccaaatc
    24781 ctctactaca tcattctccc acaagccatc cgcaaaatgc taccagccat catttctcag
    24841 tttgttaccg tgattaagga taccagtctc ctctactctg ttatcgccct acaagaactc
    24901 tttggagcca gccaaattct catgggccgt tatttcgaac cagagcaggt cttcagtctt
    24961 tacatcctga ttgccctcat ctacttcagc tttaacctag caatttctag cctgtctcat
    25021 atgctagcca aacgttggca acaagctgca gaataatact cttcgaaaat ctcttcaaac
    25081 cacgtcagct ttaccttgcc gtactcaagt acagcctgca gctagcttcc taatttgctc
    25141 tttgattttc attgagtata aacagccact ctccagttca attggagagt tttttgatga
    25201 tagtagatat ttatagacct caatttgaca ccatacattt tctatgataa aatgtaacaa
    25261 atttatttac aacaaatcga aatctaaatg aaatggaatt ttctatgaaa tcagtcaaag
    25321 gactactctt tatcatagct agttttatct tgactctttt gacttggatg aacacttctc
    25381 cccaattcat gattccagga ctagctttaa caagcctatc tctgactttt atcctagcca
    25441 ctcgtctccc actactagaa agctggtttc acggtttgga gaaggtctac accgtccaca
    25501 aattcacagc ctttctctca atcatcctac taatctttca taactttagt atgggcggtt
    25561 tgtggggctc tcgcttagct gctcagtttg gcaatcttgc catctatatc tttgccagca
    25621 tcatccttgt cgcctattta ggcaaataca tccaatacga agcttggcga tggattcacc
    25681 gcctggttta cctagcctat attttaggac tctttcacat ctacatgata atgggcaatc
    25741 gtctccttac atttaatctt ctaagttttc ttgttggtag ctatgccctt ttaggcttac
    25801 tagctggttt ttatatcatt tttctatatc aaaagatttc cttcccctat ctagggaaaa
    25861 ttatccatct caaacgctta aatcacgata ctagagaaat tcaaatccat cttagcagac
    25921 ctttcaacta tcaatcagga caatttgcct ttctaaagat tttccaagaa ggctttgaaa
    25981 gtgctccgca tcccttttct atctcaggag gtcatggtca aactctttac tttactgtta
    26041 aaaattcagg cgaccatacc aataatatct atgataatct tcaagccggc agcaaagtaa
    26101 ccctagacag agcttacgga cacatgatca tagaagaagg acgagaaaat caggtttgga
    26161 ttgctggagg tattgggatc acccccttca tctcttacat ccgtgaacat cctattttag
    26221 ataaacaggt tcacttctac tatagcttcc gtggagatga aaatgcagtc tacctagatt
    26281 tactccgtaa ctatgctcag aaaaatccta attttgaact ccatctaatc gacagtacga
    26341 aagacggcta tcttaatttt gaacaaaaag aagtgcccga acatgcaacc gtctatatgt
    26401 gtggtcctat ttctatgatg aaggcacttg ccaaacagat taagaaacaa aatccaaaaa
    26461 cagagcttat ttacgaagga ttcaagttca aataagtaat catactctcc tgggatttcc
    26521 caggagaatt ttttatagcc aaatgaaata agaccggaac gatggaattt tggcattcaa
    26581 atcaattgat aagaatattt tatagtggag tgaaatagga tacgaacaaa ttgattagga
    26641 aaatcaaatg aatttataga aatcttttag cagttatgtt gtcctattct agtttcaaaa
    26701 cgctatagaa gcagcattgt gctagtctag attcagttta ctatactaaa acgagtagct
    26761 tgaaatcaaa aaacccaccc tcacaggcag gttttatctg tattattcag ctagattatg
    26821 ctttaccttc tgaaccgaat acgtcgatac gttcttcaac cgatgcttgg atagctttta
    26881 caccgtcagc caagaattta cgtgggtcga agagtttttt cttgtcgtat tctgcttcgt
    26941 ttgcttcgta gtcacgagca aatttacgag ttgcgttagc gaatgcgatt tggcattctg
    27001 tgttaacgtt aactttggca acaccaagtt tgatagctgc ttggatttgc tcatcaggaa
    27061 tacctgatcc accgtgcaat acgattggga atcctggaag agcttctgtc aatttttgca
    27121 agtggtcaag gtcaagacct tcccagttta ctgggtaagg gccgtggatg ttaccgatac
    27181 cagctgccaa gaagtcgata ccagtttcaa ccattgcttt agcgtcttcg attggagcca
    27241 attcaccttt accgatgatt ccgtcttctt caccaccgat agtaccaact tcagcttcta
    27301 ctgagatacc tttagcgtgt gctttttcaa caacttcttt agccaattta aggttttctt
    27361 caactggaag gtgtgaaccg tcaaacatga ttgaagtata accaacttcg atacactcaa
    27421 gtgcatcttc gtagtgaccg tggtcaaggt ggatagctac tggtacagtg atacccattg
    27481 attcaacaag gttagcgatc aagttgcgag caactttgta accacccatg tatttagcag
    27541 cacccattga agtttggatc aaaactggag cttttttagc ttctgctgcg cgcaagatag
    27601 cttgagtcca ctcaaggttg tttgtgttaa atccaccaac tgcataaccg ttgtcacggg
    27661 ctgcttggac aaatttttct gctgaaacga ttgccatttt atcaggcctc ctgtatattt
    27721 ttatgggtca tcccatttac attgttcatt ttatcacttt ttgccaaaaa aatctagttt
    27781 ttcccgcagt ttcgattgat tttcttctaa ctccatctat gtaaaccctt tctctcccta
    27841 gtcttggacg acttttggaa aatctataaa gaaggttaaa ctattctcct ccatctcgaa
    27901 acgataagct aatttttcat gttctaatag actcttaacc acaaagagcc ccataccaga
    27961 ccccttgacc ttgcgactgg cattgtcaga aaaagactgg gctagttttt cttgttcctc
    28021 tgagctacag ctattttcga taaaaagttc tccttctctt tctccaattc gaactaagcc
    28081 acctggaaca gagtgcttaa tggcattgct gatgagatta gaaagaatca acttcataac
    28141 tgatgggttt agataagcct gctgatgggt caaactattg tctatctgga gctctctttc
    28201 cttggctagc aaggcataat ctttgaccag attttgcgtc atctggagga ggtcaattgt
    28261 ttccctatca tctcgcaatt cctgcacaga agagagggaa agtatctgca gaacatggtg
    28321 attgagttca tccacaatcc ccaaggcaac tcccagatac tggtctctat ccttataacg
    28381 accgatattc tctctcatat tttcgattag gattttcaaa ctagccagcg gtgttttcaa
    28441 ttcatgagaa gctcctcgta ggaattcgac cttcatcttc tccagctgga gaatggcttc
    28501 attcttttca tgcaagtccg caataacagt caagagatgc tggtagaggc tattgatttg
    28561 ttccttgaga ttacctatct catccttaga atccacgcgc aatcgcactt gggaatccag
    28621 gtccatcatc cgacgggtca cccgcttgat ttccaaaatc ggtgcaacaa tagtccgagc
    28681 gtagatgtag gccaccaaaa gggaaatcag aaaggaggcc agcaaggtat agggaagaaa
    28741 ctggagactg atttgctccg cttccttttg taaatccatg gaagctagaa actggagaat
    28801 catagtacca ccgtcttgcg ttttcacctc gcgctcctca ataaagagag aggttgtctg
    28861 gcggtctgtg tccagaggaa gactgtcctt gacttctaac ttgtcctcgg tcatctcacc
    28921 tttgacggtc cccttgatat cactagtctg ggaatacaag tctaacactt gctcgatact
    28981 ctgcctatct ttcccttcta gggactgggc aatggctgtt gccttttgac caatggtttc
    29041 ctgacgatga ctcagataag tcgaaggaaa aagaaaataa atagctaaat gaaggcagat
    29101 aaccagaaca ctaaatatcg agaaggtata gataaatatc tttgcaaata aacctgttcg
    29161 tttcattttc gctccaattt ataaccaaca ttgcgcacag tgaggataca atccaagtct
    29221 agctttttcc gcaattcctt gatataaaca tcaataacac ggtcaaaggg aacctcatct
    29281 gtcgctttcc agacggcatc gataatctga gaccgagtca aggcccggcc ttcatttttc
    29341 actagatagt ccagaatttc caactctttg gcattgatag gcacttcttg acctgcgagg
    29401 cttgcactgt agctttcaaa gtccaccttg gtagccttgt aagaaaagat tcgtcctgta
    29461 tcgtagtagc gcttgaaaat cgcgtccacc ctcactttta agagggagag ggagaaaggt
    29521 ttttccagat agccatctgc caaagaggca aaggcactca tcttgtattc ctcatcctga
    29581 aaagctgtca acatcaagac aggaacctga ctggttttac gaatctcagc tagaacttct
    29641 aagccgttaa gcttgggcat ctggatatcc agtaaaacca gggccacctc atagctagaa
    29701 aattgctcca gagcttcctg accgtccgct gcctcaatag tttcatagcc acaatccgtc
    29761 aaataatcac tgaccccctc acggatcatc tcttcatctt ctacaattaa aattttcata
    29821 ctttaactgc tctctatttt ttatttttct tagaataaat acctactcta ttttctatta
    29881 tagtctcttg ctggcctttt gtctgtaagt aactgatcac tagataaaac gttgtgaaat
    29941 tcctttctca taaattccat agctttagta tattatattt aagcaataaa gtacaaagaa
    30001 aacaactgaa agcaatgatt ttcaccactg ctttcggatt tattttgaat tgttaaatag
    30061 ccattcctat ccactattct tgaatagaaa cacaagatgc aatctttatt ccagactcat
    30121 tttttcaaat ttattcacca tccagcaaga gctcttttgg ttgttttcta aggagattgc
    30181 ttgaagcaag cgccataacg agaaccacta gaaccaaggc aaggacaaaa atgatgataa
    30241 agtctgatgt ctgaatagaa atgtctaggc tcgacaaggt cttgctaaag ccatctactt
    30301 ctgcaccacc accaaggtta gaggcttgag ccgccttact agcctgtttg gcaacacctg
    30361 aagtcacatt ggcaaggaca gtgtttccaa ttgcacgggc agtgtaatta gctaggaagt
    30421 aagcagaaac tagagcaggg atagcaatca agatagattc ggtgatgaat tgacccaaga
    30481 tacttgcctg cttgaggccg atagagagga gaattcccac ttccttgcga cgggcgttga
    30541 tccaaaggct gagcaagagg gcaaggagga gaactgagaa gctcaagcta ccccagaaga
    30601 ggaggttggc catcttgtac ataccagaga tagattgctc aagagctggg tagttagagg
    30661 agctcttgac gagtgtgtag ctcttccagt tgataccact gatgccattc aactctttca
    30721 taacatcatc caagttcttg tctgctgtta caaagaaggt tgcgtcccca taaatggctg
    30781 tgtcttctgt gtatccataa agttttgcag cagtgtgaat gtctgtaata gctgtgtttt
    30841 cgtaaagttc ttgtgagtag gttactgctg acttattatg accatcaaag agtcccttga
    30901 ttgtcacttc aactgtttcc ttggctcctt tttcattatc tgcatcgtag atattagagt
    30961 ccagtttaac cttgtcccct actttccagc cgtgtttggc tgccaagtcc ttgtgcaaga
    31021 ggattttatc cttgtcgtcg ttggttaagt gctctccttc gactagttta taagaaccag
    31081 agacaaactt gtcttcttta gaggagtcat tgacacctgt aatcatcaag ctacttccaa
    31141 aacgtttggc acgatcagca gtgagattct tcttggtttc tggcgtttca atcaggtcat
    31201 atccagtcaa atctccgata gcgttgatac gtttgacata agactcaatg gccttgtttt
    31261 cggtgatttt tttgatgtct tcacccttga tattcccagc accgcgaggc gttccttggt
    31321 tgacgcgacg attgatttgc atggagaagc tattggtgat atttttaaag gtctcctgag
    31381 aagccttggc agtagctccc ttgattgaca agccgaccaa actcaagctc gccatgagga
    31441 gaataatcag gaagatgaca atcgatttga aaaacttcct tgtaacatag gcaaatgcgt
    31501 tgtgtaacat agattccctt tctagatttt gttttaatca ttctatcaaa ataagctcaa
    31561 attatttact agtattgcgc gtttcagtca gtttcttatc ctttaattca agtgtaatat
    31621 ctgacgcttg tgccacttct ttactgtgag ttacgacaat cacacattta cctgttttct
    31681 gggcaagtga tttgagtagt tcgacaatat ctccagcagt tttaggatcc agatttcctg
    31741 ttggctcatc agctagaata actggagctt ctgagaccaa actgcgagca atggcaacac
    31801 gttgctgttg accacctgat aactggagaa cattccgctt gatctggctt tcatccaaac
    31861 caagctcaag aagtgtattc ttgcttgcct ttttgttgac caatcggata ttttccagcg
    31921 gagaaagata atctatcaag ttataatttt gaaagaccag ggaaatatgg tgcatgcgat
    31981 ggtaagaata gcccttctta cgaatatcct ctccttgaaa aaggatagaa ccttcaacag
    32041 gactatctag accagcaagt agggacaaga gtgtggattt tcctgctcct gactccccaa
    32101 taatactgta aaattttccg ggttcaaaat tataatcgat ctgatatagg actgcttcag
    32161 cagtattctt ataacggtag gtaacatctt gtaattgtaa taaagtcatg atttctcctt
    32221 cttaactaat agatgataaa atttctttcg gtgattttct aaataagaat aggaaacaaa
    32281 gggttacaga taagcaacta agcagaacta gaaaaacata ggattctgca aaagataaga
    32341 tactagttga taaactgctt gctttggcta gtgtatcttg taagcttgcc tgatctccac
    32401 ttgctagtag agtttggagt aggtaagttg tgattccgtt tcctgcaaca aatgctggaa
    32461 gcaaagctcc aagagatacc aaaactacct ctaaacagaa ttgtaggaag atcgagccct
    32521 tgccttttcc aagtgcaagt aaaatcccca cttcatagac ccgttctctc aaccagagag
    32581 acaaaaccag aattaaggct ccagctcctg ctatcaacat cccataaagg aagatggtca
    32641 ggaaggtttg gaaagttgca actgagtctt tgatttgttc aaaagccttg ttttcctttt
    32701 cgacttggta gccttgattt tccaaggcca agttttctac ctgcttcatg agtccgtcca
    32761 tttccttagg attttctaca tagaagcgtg ctgcactgac ttgagcttca ctattgccca
    32821 aaagggtttg gctactttca tagtctgtaa agacttgatt ttcactgaag tcagaagaca
    32881 agcctgtgaa tttctcttgt tttttaccag aaaagatgcc aataatctca aactctactg
    32941 tttgtccttt tccagattca gactgaccag catccaagcc aatcttgtca tgaagtgaaa
    33001 gaccgttctt cttagccaat tcttcgtgga taaggatttt cttggaatcc cctttttgaa
    33061 ggtgtcgccc ttcttttaga ttgaaagccg aactggtaaa ggttacatcc ttggatgaat
    33121 cctcaagagc cgttaagcta accaagttat tgtctgcagc tgataaatca tcacgctcca
    33181 cgctctgctc gccagtcact gcttccttgt cttttagttt tgcgaccgtc tcaagttcag
    33241 gagagacatt ttccagcccc ttaatcttgc ttacagatgc taggtctgac aacttgaatg
    33301 tctgaccatt ctctatcttc ttaatagaaa aagatgtatt gagtgattta taaagattgc
    33361 tttctactgt tttgttggac ttcatcagag tcaaacaggc tgaaattccg gccaataaga
    33421 ccaataaaat cagaaataaa ataaaacttc tcagtcgctt tctgctgaca taagcccaag
    33481 atctttggat tggattcatt tgtcacctcc atatttgtaa gactattata aaacccaaat
    33541 atgaaatgtt tatgaaatac gaaaaaaaaa
//
LOCUS       DAVKWZ010000018        33322 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000018 DAVKWZ010000000
VERSION     DAVKWZ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33322
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.018"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            69..566
                     /locus_tag="VW854_000903"
     CDS             69..566
                     /locus_tag="VW854_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV0262166.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTM
                     FEEKS"
     gene            566..2221
                     /gene="dnaX"
                     /locus_tag="VW854_000904"
     CDS             566..2221
                     /gene="dnaX"
                     /locus_tag="VW854_000904"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEV0262167.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVNLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNAGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            2249..2443
                     /locus_tag="VW854_000905"
     CDS             2249..2443
                     /locus_tag="VW854_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEV0262168.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            2593..3363
                     /gene="sufC"
                     /locus_tag="VW854_000906"
     CDS             2593..3363
                     /gene="sufC"
                     /locus_tag="VW854_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEV0262169.1"
                     /translation="MSVIEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            3391..4653
                     /gene="sufD"
                     /locus_tag="VW854_000907"
     CDS             3391..4653
                     /gene="sufD"
                     /locus_tag="VW854_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEV0262170.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKISYLERLESRGEGSDKVTANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            4664..5890
                     /locus_tag="VW854_000908"
     CDS             4664..5890
                     /locus_tag="VW854_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV0262171.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILAEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSSVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            5877..6317
                     /locus_tag="VW854_000909"
     CDS             5877..6317
                     /locus_tag="VW854_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEV0262172.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDII
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQDQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            6371..7783
                     /gene="sufB"
                     /locus_tag="VW854_000910"
     CDS             6371..7783
                     /gene="sufB"
                     /locus_tag="VW854_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEV0262173.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(8014..9255)
                     /locus_tag="VW854_000911"
     CDS             complement(8014..9255)
                     /locus_tag="VW854_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEV0262174.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            9358..10467
                     /locus_tag="VW854_000912"
     CDS             9358..10467
                     /locus_tag="VW854_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV0262175.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRIQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDCLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
     gene            11113..11460
                     /gene="ssrA"
                     /locus_tag="VW854_000913"
     tmRNA           11113..11460
                     /gene="ssrA"
                     /locus_tag="VW854_000913"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(11630..11717)
                     /locus_tag="VW854_000914"
     tRNA            complement(11630..11717)
                     /locus_tag="VW854_000914"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11681..11683),aa:Ser,seq:gga)
     gene            11879..12619
                     /locus_tag="VW854_000915"
     CDS             11879..12619
                     /locus_tag="VW854_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV0262176.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            12616..13527
                     /gene="pfkB"
                     /locus_tag="VW854_000916"
     CDS             12616..13527
                     /gene="pfkB"
                     /locus_tag="VW854_000916"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEV0262177.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVEPVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            13524..15476
                     /locus_tag="VW854_000917"
     CDS             13524..15476
                     /locus_tag="VW854_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV0262178.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTEHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGALYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            15640..17865
                     /locus_tag="VW854_000918"
     CDS             15640..17865
                     /locus_tag="VW854_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEV0262179.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKAQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEIAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(17910..18338)
                     /locus_tag="VW854_000919"
     CDS             complement(17910..18338)
                     /locus_tag="VW854_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262180.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTVATIASILGLITAGITIMN
                     ALDNNKNIKKEPTTSQTTTIVKEIQKESPKENTSPTKETNTSQEKTQQEETPKSSVKE
                     EKKEDQKTATQDSSTPASSKPATENEKQSNAPTSENKSNQ"
     gene            18476..19618
                     /locus_tag="VW854_000920"
     CDS             18476..19618
                     /locus_tag="VW854_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV0262181.1"
                     /translation="MIYFDNSATTRPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWIIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKAQGFEVDFAPVDNKGFVDVEALEDLIRPDTTLVSIMAVNNEIGSVQPIEAI
                     SKLLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     DYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAQSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            19627..20841
                     /gene="thiI"
                     /locus_tag="VW854_000921"
     CDS             19627..20841
                     /gene="thiI"
                     /locus_tag="VW854_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEV0262182.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYPQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSAVQEIMQDIYKE
                     GMTFKISSKRSDHTFELDSRELNQTLGGAVFEAIPNVQAQMKSPDINLQVEIREEAAY
                     LSYETVRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            20976..21789
                     /locus_tag="VW854_000922"
                     /pseudo
     CDS             20976..21789
                     /locus_tag="VW854_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            21811..22146
                     /locus_tag="VW854_000923"
     CDS             21811..22146
                     /locus_tag="VW854_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEV0262183.1"
                     /translation="MHIEHLSHWSGHPNREMYLNRYGHGGIPVVVFASSGGSHNEYYD
                     FGMIDACASFIEEGRVQFFTLSSVDSESWLATWKNAHDQAEMHRAYERYVIEEAILLS
                     STRQVGLMA"
     gene            22143..22550
                     /locus_tag="VW854_000924"
                     /pseudo
     CDS             22143..22550
                     /locus_tag="VW854_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855897.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            22563..23729
                     /locus_tag="VW854_000925"
     CDS             22563..23729
                     /locus_tag="VW854_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV0262184.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIGRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNEVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFEPQYCLATSRRANAHYVY
                     SEENLLAKYSQQFKVKKIMPEAFAELQGDYFYMLTTPSRQEMDQMIADFGQRQE"
     gene            24063..25556
                     /locus_tag="VW854_000926"
     CDS             24063..25556
                     /locus_tag="VW854_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV0262185.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESNAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPIRDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            25569..26462
                     /locus_tag="VW854_000927"
     CDS             25569..26462
                     /locus_tag="VW854_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV0262186.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLRDHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKRLITKRK
                     FQLDELNLLVKSRFNEMFGENKIFESIDNLFDIIDGDRGKNYPKSDELFSEEYCLFLN
                     TKNVTKNGFSFDTKQFITKTKDKLLRKGKLERYDIVLTTRGTVGNVAYYDELIKYKHL
                     RINSGMVILRPKTPNHNLLVR"
     gene            26536..29887
                     /locus_tag="VW854_000928"
                     /pseudo
     CDS             26536..29887
                     /locus_tag="VW854_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            30131..30232
                     /locus_tag="VW854_000929"
                     /pseudo
     CDS             30131..30232
                     /locus_tag="VW854_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(30473..30943)
                     /locus_tag="VW854_000930"
     CDS             complement(30473..30943)
                     /locus_tag="VW854_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV0262187.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAKNDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(30960..33233)
                     /locus_tag="VW854_000931"
     CDS             complement(30960..33233)
                     /locus_tag="VW854_000931"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEV0262188.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELKVKLPRKIELEKPQLNLVQPQGQAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCHAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCKTFSLGQEEQ
                     AIQNQYPDKDFERYSKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
BASE COUNT        10019 a         6013 c         7298 g         9992 t
ORIGIN      
        1 aaaatccttt gtttactaaa caagggattt ttcttttgtc tctgctcctt ttttgatata
       61 atagttctat gttaaaatca gaaaaacaat cacgttatca aatgttaaat gaagaattgt
      121 ccttcctatt ggaaggcgaa accaatgttt tggctaatct ttccaacgcc agtgctctca
      181 taaaatcacg ttttcctaat accgtatttg caggctttta tttgttcgat ggaaaggaat
      241 tggttttagg tcccttccaa ggaggtgttt cctgcatccg tattgcacta ggcaagggtg
      301 tttgtggtga ggcagctcac tttcaggaaa ctgttattgt tggagatgtg acgacctatc
      361 tcaactatat ttcttgtgat agtctagcta aaagtgaaat tgtggtgccg atgatgaaga
      421 atggtcagtt acttggagtt ctggatctgg attcttcaga gattgaggat tacgatgcta
      481 tggatcgaga ttatttggaa caatttgtcg ctattttgct tgaaaagaca acatgggact
      541 ttacgatgtt tgaggaaaaa tcttaatgta tcaagcactt tatcgaaaat atagaagtca
      601 aaacttctcc cagttagttg gtcaagaagt tgtggctaag actcttaaac aagcggtgga
      661 gcaagagaaa ataagtcacg cttatctttt ttctggtcct cgtggaacgg gaaaaaccag
      721 tgttgctaaa atctttgcca aggctatgaa ctgtcccaat caagtgggtg gcgaaccttg
      781 caataactgc tatatttgtc aagcagtgac ggacggtagt ttagaagatg tcattgaaat
      841 ggatgcagct tctaataatg gggtagatga aattcgcgaa attcgtgata aatctaccta
      901 tgcgcctagc cttgctcgtt ataaggttta tatcatagat gaggttcaca tgctgtctac
      961 aggggctttt aatgccctcc taaagacgct ggaagaacca acacagaatg tagtctttat
     1021 tttggccact actgaattgc acaagattcc tgctactatt ctatcccgtg tgcaacgttt
     1081 tgagtttaaa tcaattaaga cacaggatat taaggaacat attcactata tcttagaaaa
     1141 agaaaatatc agttctgaac cagaggctgt ggaaatcatt gccagacggg cggaaggtgg
     1201 aatgcgggac gccttgtcta ttttggatca agccctgagt ttgacacagg gaaatgagct
     1261 gacgactgct atctctgaag aaattactgg caccattagc ctatcagcct tggatgatta
     1321 tgtggcggcc ttgtctcaac aggatgttcc caaagctttg tcttgcttga atcttctttt
     1381 tgacaatggt aagagcatga ctcgttttgt gaccgatctt ttgcactatt taagagactt
     1441 gttaattgtt caaacagggg gagaaaatac tcatcatagt tcagtctttg tagaaaattt
     1501 ggcacttcct caaaaaaatc tgtttgaaat gattcgctta gcaacagtga atttagcaga
     1561 tattaagtct agtttgcagc ccaagattta tgctgaaatg atgaccgtcc gtttggcgga
     1621 aatcaagccc gaaccagctc tatcaggagc ggttgaaaat gaaattgcta cgctgagaca
     1681 ggaagttgcc cgtctcaaac aagagctttc taatgcaggt gcggttccta aacaagttgc
     1741 accagctcct agtcgaccag ctacgggcaa aacagtctat cgtgtcgatc gcaataaagt
     1801 gcaatctatc ttacaagagg ccgtcgaaaa tcctgattta gcacgtcaaa atctaattcg
     1861 tttgcagaat gcctggggag aggtaattga aagtctaggt gggccggaca aggctctgct
     1921 agttggttct caaccggttg ctgccaatga acaccatgct attcttgctt ttgagtctaa
     1981 cttcaatgct ggtcaaacta tgaaacgaga caatctcaat accatgtttg gtaatatcct
     2041 cagtcaggcg gcaggttttt cacctgagat tttagctatt tccatggagg aatggaaaga
     2101 agttcgcgca gccttttcag ccaaagccaa atcttctcaa actgaaaaag aagtagaaga
     2161 aagcctgatt ccagaaggat ttgaattttt ggctgataaa gtgaaggtag aggaagacta
     2221 aagaaagatt tcatgataca ataagtttat gaataaacaa caatttatta ttatggcgct
     2281 atttacagct gctgagacct attttttcaa tgaagcctgg atgactggcc gctatattat
     2341 ggcagccttt tgggcaattt tactctttag aaatttccga gtcagttatg tgatgggtaa
     2401 aatcgttgat gtcatcgatc agcattttaa taggaaagac tagccctcag cttccagaca
     2461 aaatcaaagc cttttaggct tttttttgtt atactagaaa agtatattta tagaattttt
     2521 gctctatttc tggggaaatc agacgttttt ctagtaagta ctgtaaaagt tttgaaaaag
     2581 aaaggaacta tcatgtcagt aatagagatc aaagatcttc acgttgagat tgaaggaaaa
     2641 gaaattttaa aaggggttaa cctgaccctg aaaacaggag aaattgccgc tatcatggga
     2701 ccaaatggta caggtaaatc gactctttct gccgctatca tgggaaatcc aaactatgaa
     2761 gtaactaaag gtgaagtttt gtttgatggc gtaaacatcc ttgagttgga agtggatgag
     2821 cgtgcgcgta tgggactttt ccttgctatg caatacccat cagaaatccc tggaattacc
     2881 aatgctgagt ttcttcgtgc cgctatgaat gcgggtaaag aagatgatga gaagatttca
     2941 gttcgtgagt ttattactaa gctagatgaa aaaatggaat tgctcaacat gaaagaagaa
     3001 atggcagagc gttacctcaa cgaaggcttc tctggtggtg agaaaaaacg caatgaaatt
     3061 cttcaacttt tgatgttgga gccaacattt gctcttttgg acgagattga ctcaggtctt
     3121 gatattgacg ctcttaaagt tgtgtctaaa ggtgtcaatg ccatgcgtgg tgaaggtttt
     3181 ggtgctatga tcatcactca ctaccaacgt cttttgaact atatcacacc tgatgtggta
     3241 cacgtgatga tggaaggtcg tgttgtcctt tctggtggtc cagaattggc tgcgcgtttg
     3301 gaacgtgaag gatacgcaaa actagctgaa gaacttggct acgactacaa ggaagaattg
     3361 taattccctc gtatctttta ggagaagtaa atgactagag aaaatattaa acttttttca
     3421 gaaatgcacg ctgaaccaag ctggttggct gatctccgtc aaaaagcttt tgacaagatt
     3481 gagactttgg aattaccagt tattgagcgt gtcaaattcc accgttggaa tctgggtgat
     3541 ggaacgatta cagaaaatga gccatcagca aatgttccag atttcacagc tttagatcat
     3601 cacttgaagt tggtgcaagt aggaactcaa actgttttcg aacaaactcc agttgagtta
     3661 gctgaacagg gtgttgtctt cacagacttt cactcagctt tagaagaaat tccagagctg
     3721 atcgaagaat tcttcatgtc atctgttaag tatgatgatg acaagttggc ggcttaccac
     3781 acagcttact ttaacagtgg tgctgtactc tatattccag ataacgtaga aatcacagag
     3841 ccaattgaag gaattttcta ccaagatagc gatagcaatg tgccgtttaa caagcatatt
     3901 atgattatcg ctggtaaaaa ttctaagatt agttatctgg agcgtttaga gtcacgcggt
     3961 gaaggaagtg acaaagtaac tgccaatatc acagtggaag tgattgcacg ttctggtgcg
     4021 caagtcaagt ttgctgctat cgaccgtcta ggtgaaaacg tcactgccta cattagccgt
     4081 cgtggtaaat taggcaacga tgcaagtatt gactgggcta tcggtgtcat gaacgaagga
     4141 aatgtcgttg ctgattttga tagtgacttg attggtaatg gtagccatgc tgacctcaag
     4201 gttgtagctc tttcaagtgg tcgtcaggta caagggattg atactcgtgt aactaactat
     4261 ggctgcaact ctatcggaaa tatcctacaa catggggtta tccttgaaaa agcaactttg
     4321 actttcaatg gtatcggcca catcatcaag ggtgctaagg gagcagatgc gcaacaagag
     4381 agccgtgttc tcatgctttc agaccaagcg cgttcagatg ctaacccaat tcttttgatt
     4441 gatgaaaatg atgtcactgc aggacacgca gcgtctatcg gacaagttga tccagaagac
     4501 atgtattacc tcatgagtcg tggattggat aaggcaactg cagagcgttt ggttgttcgt
     4561 ggtttccttg gatctgttat cgtggagatt ccagtcaagg aagttcgtga tgaaatgatt
     4621 gcaactatcg aagagaaatt gtcaaaacgc taaggggaag cctatgttag atgtagaagc
     4681 gattcgcaag gattttccaa ttttagatca gattgtcaat gatgaacctc tggtctatct
     4741 ggacaatgct gcgacgacac aaaaaccact agtagttctg aaagctatta acagttacta
     4801 tgagcaggac aatgccaatg ttcaccgtgg tgtccatacc ttagcggaac gagcgacagc
     4861 ttcttatgaa gctgctcgtg aaaccattcg taagtttatt aatgcaggct ctacaaagga
     4921 agttctcttt accagaggaa cgacaaccag ccttaactgg gtggcacgct ttgctgagga
     4981 aattctcgct gagggagacc aggtcttgat ttcagtaatg gaacaccatt ctaatatcat
     5041 tccatggcag gaagcttgtc gaaagactgg agcagagctt gtctatgtct atcttaaaga
     5101 cggtgccttg gatatggagg atttgcgagc taaattgact gataaggtta aatttgtttc
     5161 cctagctcat gcctccaatg ttcttggtgt ggtcaatccg atcaaggaaa tcactcaatt
     5221 agcccaccaa gttggggcaa ttatggtagt ggatggtgct caatctacac ctcatatgaa
     5281 gattgatgtc caggacttgg atctggactt tttcgccttt tcgggtcaca agatggctgg
     5341 tccgactggt atcggtgtcc tttacggcaa agaaaagtat cttgagcaaa tgtctccagt
     5401 agaatttggc ggcgagatga ttgattttgt ctacgagcaa tctgctagtt ggaaggaatt
     5461 gccttggaaa tttgaggctg gaacgccaaa tatggcagga gctattggac ttgcgactgc
     5521 agttgattat ctggaaaaga ttggtatgga tgccgttgaa gctcatgaac aggaattaat
     5581 tgcgtacgtc tatccaaaac tgcaggcaat tgagggattg accatttacg gttctcagga
     5641 tttggctcaa cgttcgagtg ttattgcctt taacctaggt gatctccatc ctcacgatct
     5701 tgcgacggct ctggattatg aaggagtggc tgttcgtgct ggtcaccatt gtgcgcaacc
     5761 cttgcttcag tatttggaag tcccagcaac agctcgtgca agtttttata tctacaatac
     5821 caaggcagat tgcgacaaac tagtcgatgc cctacaaaag acaaaggagt ttttcaatgg
     5881 cactttctaa actagatagc ctttatatgg cagtggtagc agaccattcg aaaaatccac
     5941 atcaccaagg gaagttagaa gacgctgagc aaatcagtct caacaatccg acttgtgggg
     6001 atatcatcaa cctctctgtc aagtttgatg cagaggaccg tttggaagat attgcttttc
     6061 taaattcagg atgcacgatt tcaactgctt ctgctagtat gatgacagat gccgttttag
     6121 gaaaaaccaa acaagaaatt ttagaactgg cgactatttt ttctgaaatg gttcaagggc
     6181 aaaaagatga gcgtcaagac caacttggag acgcggcatt cttgtcaggt gttgccaaat
     6241 tccctcaaag aatcaagtgt gcaaccctag cttggaatgc ccttaagaaa acaattgaaa
     6301 atcaagaaaa acagtaagac aagtttcttt tgtcttatga attattagaa atgaagaaag
     6361 aaaggatatt atggctgaag aaagagtaga accaaaacca attgaccttg gtgaatataa
     6421 atttggtttc catgacgatg tagagcctgt cttatcgaca ggaaaaggac tcaacgaagg
     6481 tgttattcgt gaattatctg ctgctaaggg tgagcctgag tggatgttgg agttccgttt
     6541 gaagtcttat gaaaccttca aaaaaatgcc catgcaaact tggggagcag acttgtcaga
     6601 gattgacttt gatgacttaa tctactatca aaaaccatct gacaaaccag cccgttcttg
     6661 ggatgatgta cctgaaaaga ttaaagaaac ctttgaacgt atcgggattc cagaagctga
     6721 acgtgcttat ttagcagggg cttctgccca gtacgagtca gaagtggttt accacaacat
     6781 gaaggaagag ttccaaaaat taggtattat ctttacagat acagattccg cactcaagga
     6841 atacccagac ttatttaaac aatactttgc gaagttggta ccgccgacag ataacaagtt
     6901 ggcagccctc aactcagcag tatggtcggg tggaactttt atctacgttc caaaaggtgt
     6961 caaggtagat attccacttc aaacttattt ccgtatcaat aacgaaaata taggtcagtt
     7021 cgaacgtacc ttgattatcg ttgatgaggg agcaagcgtc cactacgtag aaggatgtac
     7081 agcaccaaca tattcaagca atagcttaca cgctgccatt gtagaaattt ttgctttgga
     7141 cggagcttat atgcgttata caactatcca aaactggtct gataacgtct ataacttggt
     7201 aacaaagcgt gctaaggctc aaaaggatgc cactgttgag tggattgatg gaaacttggg
     7261 tgccaaaacg actatgaaat atccatctgt ttaccttgat ggagaaggag cgcgtggtac
     7321 catgctctct atcgcctttg ctaatgcagg gcaacaccaa gacacgggtg ctaagatgat
     7381 tcacaatgct ccacatacca gctcgtctat tgtgtctaaa tctatcgcta aaggtggagg
     7441 aaaggttgac taccgtggac aagtcacctt taacaagaac tctaagaaat ctgtttccca
     7501 cattgaatgt gataccatta tcatggatga cttgtcagca tcagatacta ttccatttaa
     7561 tgaaattcac aactcgcaag tggctttgga acacgaagcc aaagtatcta agatttcaga
     7621 agagcaattg tattatctca tgagccgtgg attgtcagaa tctgaggcaa ctgaaatgat
     7681 tgtcatggga tttgtagaac cctttacaaa agaacttcca atggaatacg cagttgagct
     7741 gaaccgcttg attagctatg aaatggaggg atcagttgga taaaatttga ttttatactc
     7801 ttcgaaaatc tcttcaaacc acgtcagcat cgccttaccg tatgtatggt tactgacttc
     7861 gtcagtttca tctacaacct caaaacagtg ttttgagcaa cctgaggcta gcttcctagt
     7921 ttgttctttg attttgagta ttagatttac tcaaaatcaa ggattttgaa gatgaacttg
     7981 tatcaaaaaa tcgcggttta aaatcgcgat tttttataat ttctcgttaa caaagcggac
     8041 aaactgattc caccaaactt ttaagaagaa ggctttttca attttcttgt ctgctaccat
     8101 ttcgaaacta gggcgctctg tggtgatgta accttgacca atcaagtcct tgtcttcata
     8161 agtcaaatgg ccaaccactg ttccagcttc aagtggtgct gggattgctt tggaatcagg
     8221 tgtgaattga acagattggg aagattgatt cccaacacgt tcgattagat agatatcctc
     8281 tggagccact gcagttactg tatcttcttt tccatcttgt acaggggctt tgctatcttg
     8341 ataggcatcg ccttgttgaa cgattttgcg aagtgtaaat gtagaagaaa tataatccat
     8401 tagggaagat gtagctgtaa atcgagcgta aggattattg tcttgatgat ctgcatttaa
     8461 aacaactgtg atgactctca tgcctttttc gacagtagta ccaacaaaag actctccagc
     8521 cttatctgtt gttcctgttt ttagcccatc aaaaccacca cggtaagcag gcataccttc
     8581 taacatgtag ttggttgaag tgattgtcat cccagcaaaa gtagaagaag gttttttggt
     8641 gatttctaag acttgtgggt attttttgat gaggttgcga gcaacgatag cgacatcata
     8701 agcactaagc ttattttcct catctttttt agaacctggg taaatgttat cccctagagt
     8761 ttcattgtta agacctgtcg tattgacaac agtggcatcc tgaattcccc attccaagag
     8821 ttttgcccgc atcatatcga cgaaatcttt ttctgagcca gcaattttct cagctagggc
     8881 aatagcggcg ctgttggcac tagataccag agttgcttca agcaactctt cgacagtata
     8941 attacgggcc tccataggaa tattactggc ttcagaattt gtcgtcaatt gataaggata
     9001 atcagaaata tctacaggag tggagagggt aatacttccg ttttccaaag cttcatagac
     9061 cagataaaca gtaatcaatt ttgttatgga agcaatttcg acaggttgcg ttgcatcctt
     9121 ctcatagaga attttaccag tatttgcctc aacagcaatc gcatgtttag cggcaatggt
     9181 aaaatcttga gcaacagcag tagaagcacc ccctaaaaga gagacagtta acaaagttaa
     9241 aaatattttt ttcatagtag tcttattcta tcataaagaa aaaaaatatt cttgctttaa
     9301 taattcatct gttaagcttt ttgaaaatat ggtaaaataa agtgagggag gtaactcatg
     9361 tttcgtagaa ataaattatt tttttggacc acagaaattt tactcttaac catcatcttt
     9421 tacctatgga gacagatggg gtctttgatt aacccttttg ttagcgtgct taatacaatt
     9481 atgattccat ttttattagg gggctttctt tattatttga caaaccctat tgttactttc
     9541 ttaaataaag tctgtaaact caatcgtttg cttggtattt taattacctt gtgtactttg
     9601 gtctggggaa tggtcatagg tgttgtctat ctcttaccta ttttgattaa tcagttatct
     9661 agtttgatta tatctagtca aactatttat agtcgaatac aagacttaat catagactta
     9721 tctaattatc ctgcgctcca gaatttggat gtagaagcta caattcagca gttaaactta
     9781 tcctatgttg atattcttca aaatatccta aatagcgtat caaatagtgt ggggagcgtc
     9841 ttgtcagctc ttatcagtac tgttttgatt ttgattatga ctccagtttt tttggtttat
     9901 ttcttattag atggacataa attcttgccc atgcttgaaa gaacgattct aaagagggat
     9961 tgcttgcata ttgcaggctt attaaagaat ttaaatgcga cgattgctcg ctatattagt
    10021 ggagtttcga ttgacgcaat cattataggt tgtttagctt atattggcta tagtattatt
    10081 ggtttaaaat atgctttagt ttttgccatt ttttctggtg tagccaattt aattccttat
    10141 gtggggccaa gtattggttt gattcctatg atcatcgcaa atatattcac tgatcctcat
    10201 agactgctga ttgcagtgat ttatatgctt gttgttcagc aggtagatgg caatatctta
    10261 tatcctcgaa tcgtaggaag tgttatgaag gttcatccaa tcacgatttt agttttactt
    10321 ttgttgtcaa gcaatatcta tggtgtagtt ggaatgattg tcgcagtgcc aacctattct
    10381 atcttgaaag aaatttctaa gttcttatcc cgtttgtatg aaaatcataa aataatgaaa
    10441 gaacgagaaa gagaattagc taagtaaaag tcaggagaac cctgattttt ctttactgga
    10501 agtggccttt agattagaag actgaaaata agttaaagtc ttaaactaat tttcacagct
    10561 aagaatagta gaagttaatc tgataaaaat cgaaaaaacc agtggaattc tgtgtcaggg
    10621 taagttccac tggttttcat agtctattaa agttcgaatg aaacctattt atagtatatt
    10681 gaaactagaa tagtacacct ctaattctaa aacattgtta gaaatcgatt tgactgtcct
    10741 aatctattcg tcctattctt atttcatttt actatatttt ggtgcaataa gtgaaaagta
    10801 gtccgaataa tataaggatt gattttatag tttttaaact caaatgaatt aaaataaaga
    10861 gagtacgaaa attctcatct gaaagtattt tagaataatt ctcttcgtga atttcttcaa
    10921 aacagatagc ttcatcttag gtatgtgtta attggatttc gtaagctcta tttacaatcg
    10981 taaagttatg cactgagaaa gttgtagtta gtcttccagt ttgctaaatt tctcgaatag
    11041 tatctttttt aggtatgtga tttctttttg catttttgag ttagataagg tataatagtt
    11101 ttattgtctt ttggggtcgt tacggattcg acaggcatta tgaggcatat tttgcgactc
    11161 gtgtggcgac gtaaacgctc agttaaatat aactgcaaaa aataacactt cttacgctct
    11221 agctgcctaa aaaccagcag gcgtgacccg atttggattg ctcgtgttca atgacaggtc
    11281 ttattattag cgagatacga ttaagccttg tctagcggct tgataagaga ttgatagact
    11341 cgcagtttct agacttgagt tatgtgtcga ggggctgtta aaataataca taacctatgg
    11401 ttgtagacaa atatgttggc aggtgtttgg acgtgggttc gactcccacc ggctccatta
    11461 ttcctttgca ttcttttgca ttccttggta aaacgttgtt aaatcaacgt tttttatttt
    11521 tatatttggt attcctttgc attcttttgc taaaaaggga gtcacaaaca gaccctattt
    11581 taaaaaagga tacaacattt gtcgcatcct aaaaataatc ttttttcgac ggaagacatg
    11641 ggattcgaac ccacgcacgc tattacacgc ctaccgcgtt tccaacacgg cctcttaagc
    11701 ctcttgagta atcttccaat acttactcaa atagtctacc ataaaggctc ttatcttgca
    11761 ataaaaattc tagaaataag aaaaatgata gattttgaaa gaaaatgaca aaaaatgctt
    11821 gacttcgaaa taaagtatgg tagaatgaat agtgtaaacg ataacaggag gtgattcagt
    11881 gttaaaaaca gaacgtaaac aactgatttt agaggagtta aatcaacatc atgtagtttc
    11941 tctagaaaaa ttagttagtt tgctagaaac gtcagaatca acggttcgaa gagacttgga
    12001 tgagttggaa gcggaaaaca agcttcgtcg tgtgcatggt ggagcagaac tcccccactc
    12061 cttacaggaa gaagaaacca ttcaagaaaa atctgtcaaa aaccttcaag aaaagaagtt
    12121 gctggctcag aaagcagcct ctctcattaa agaaaaagat gtcatcttta tcgatgctgg
    12181 aacaacaact gcttttttga ttcatgaatt ggtcaataag aatgttacag ttgtgaccaa
    12241 ctccattcac catgctgctc agttggttga aaagcagatt ccaactgtca tggttggagg
    12301 aaacgtcaag acggcgacag atgctagtat cgggggcgtt gctcttaacc agattaacca
    12361 attgcacttt gaccgtgcct ttatcggaat gaatggtgtt gacgatggct attatacgac
    12421 tcctgatatg gaggagggag ctgtgaaaag agctattttg gagaatgcca agcagaccta
    12481 cgtcttggtg gattcgtcaa aaattggaca aacttgcttt gccaaggtag caccactcaa
    12541 acgcgctatc gttatcacta gtcaagggca tgagctcttg caggttatta aggagaaaac
    12601 ggaggtaata gaagtatgat ttatacagtc acactcaatc catccattga ctatatcgtt
    12661 cgtttggacc aagtcaaagt tggtagtgta aatcgtatgg acagtgatga taagtttgct
    12721 ggtgggaaag gaatcaatgt cagccgtgtc ttgaaacgtt tgaatatacc aaatacagcg
    12781 acgggattta tcggtggctt tactggtaaa tttatcacag atactttagc agaggaagaa
    12841 atcgagacac gttttgtcca ggtggcagaa gatactcgta tcaatgttaa aatcaaagca
    12901 gaccaagaaa cagaaatcaa cggaacgggt ccaactgttg aaccggttca gctagaagaa
    12961 ttgaaagcta ttttatctag tctgacagca gaagatacag ttgtctttgc aggttcaagt
    13021 gctaaaaatc taggcaatgt tatctataag gatttgattt ccttgacgcg ccagactggt
    13081 gcgcaagtgg tctgtgactt tgaaggacag accttaattg atagtttgga ctaccagcct
    13141 cttcttgtaa aaccaaacaa tcatgaactt ggagcgattt ttggggttaa actcgaaagt
    13201 ttagatgaaa ttgagaaata cgctcgtgag ttactggcta agggtgctca aaatgttatt
    13261 atctctatgg ctggtgatgg cgcccttctt gtcacatctg agggagctta cttcgctaaa
    13321 ccaatcaaag gaacagtcaa aaattcagtt ggagctggtg attctatggt tgctggattc
    13381 acaggtgaat ttgtcaaatc aaaagacgca gtagaagcct tcaaatgggg agtggcttgc
    13441 ggaacggcaa ctaccttctc agatgacttg gcaacggcgg aatttattaa agaaacatat
    13501 ggaaaagttg aggtagaaaa aagatgaaaa ttcaagacct attgagaaaa gatgtcatgt
    13561 tgctagattt gcaggcaact gaaaaaacag ctgtcatcga cgagatgatt aaaaatttga
    13621 cagagcacgg ttatgtaaca gattttgaaa catttaaaga aggaattttg gcgcgtgaag
    13681 ctttgacttc tactggtttg ggtgatggaa tcgcaatgcc tcacagcaaa aacgctgctg
    13741 tcaaagaagc gacagttcta tttgctaagt caaataaggg tgttgactac gagagcttgg
    13801 atggacaagc aactgacctc ttcttcatga ttgcagctcc agaaggtgcc aatgatactc
    13861 acttggcagc cttggcagaa ttgtctcaat acttgatgaa agacggtttt gcagacaaac
    13921 ttcgtcaagc aacatctgca gaccaagtta tcgaactttt tgaccaagct tcagaaaaaa
    13981 ctgaggaact tgttcaagca cctgctaatg actctggtga ctttatcgta gctgttacag
    14041 cttgtacaac aggtattgcc cacacttaca tggcccaaga agcccttcaa aaagtagctg
    14101 ctgaaatggg ggttggtatc aaagtcgaaa ccaacggtgc tagcggtgtt ggaaatcaac
    14161 taactgcaga agatatccgt aaggctaaag ctattatcat tgcagcagac aaggccgttg
    14221 aaatggatcg atttgatgga aaaccattga tcaatcgtcc agttgctgac ggtatccgta
    14281 agacagaaga gctaattaac ttggctcttt caggagatac tgaagtctac cgtgccgcta
    14341 atggtgccaa agctgcaaca gcctctaacg aaaaacaaag ccttggtggt gccttgtaca
    14401 aacacttgat gagtggtgta tctcaaatgt taccattcgt tatcggtggt ggtatcatga
    14461 ttgcccttgc cttcttgatt gacggtgctt tgggtgttcc aaatgaaaac cttggcaatc
    14521 ttggttctta ccatgagtta gcttctatgt tcatgaaaat tggtggagct gcctttggtt
    14581 tgatgcttcc agtctttgcg ggttatgttg cctactctat tgctgaaaaa ccgggtttgg
    14641 tagcaggttt cgtggctggt gctattgcca aagaaggttt tgcctttggt aaaattcctt
    14701 atgccgcagg tggtgaagca acttcaactc ttgcaggtgt ctcatctggt ttcctaggtg
    14761 cccttgttgg tggatttatc gcaggtgcct tggttcttgc catcaagaaa tacgttaaag
    14821 ttcctcgttc actcgaaggt gctaaatcaa tccttctatt gccacttctt ggaacaatct
    14881 tgacaggatt tgttatgcta gctgtgaata tcccaatggc tgcaatcaac actgctatga
    14941 atgacttcct aggcggtctt ggaggaggtt cagctgtcct tcttggtatc gtccttggtg
    15001 gaatgatggc tgttgacatg ggtggaccag ttaataaagc agcttatgtc tttggtacag
    15061 gtacgcttgc agcaactgtt tcttcaggtg gttctgtagc catggcagca gttatggctg
    15121 gaggaatggt gccaccactt gcaatctttg tcgcaactct tcttttcaaa gataaattta
    15181 ctaaggaaga acgtaactct ggtttgacaa acatcatcat gggcttgtca tttatcactg
    15241 agggagcgat tccatttggt gccgctgacc cagctcgtgc gattccaagc ttcatccttg
    15301 gttcagcagt agcaggtgga ctcgttggtc ttactggtat caaactcatg gcgccacacg
    15361 gaggaatctt cgttatcgcc cttacttcaa atgctctcct ttacctcgtt tctgtcttgg
    15421 taggagcaat cgtaagtggt gtggtttatg gttacctacg caaaccacaa gcataaaaaa
    15481 tagaaaaatg aaaagattgg accgtttggt gcagtctttt tctcttcccg aaatgcctgt
    15541 gaaatatggt ataatagaag aatggcaaac aagaatacaa gtacaacaag acggagaccg
    15601 tctaaagcag aactggaaag aaaagaagcg attcaacgaa tgttgatttc gttaggaatt
    15661 gcgattttat tgattttcgc agccttcaaa ttaggggctg caggtataac cctttataat
    15721 ttaattcgct tgctagtggg tagcctagct tatctggcga tattcggcct attaatctat
    15781 ctcttctttt tcaagtggat acgaaaacag gaaggactct tatctggctt tttcaccata
    15841 tttgctggct tactcttgat ttttgaggcc tacttggttt ggaaatatgg tttggacaag
    15901 tccgttctaa aagggaccat ggctcaggtt gtgacagatc tgactggttt tcgaacgact
    15961 agctttgctg gagggggctt gatcggggtc gctctttata ttccaacagc ctttctcttt
    16021 tcaaatatcg gaacttactt tattggttct atcttgattt tagtgggttc tctcctagtc
    16081 agcccttggt ctgtttacga tattgctgaa tttttcagta gaggctttgc caaatggtgg
    16141 gaagggcacg agcgtcgaaa agaggaacgc tttgtcaaac aagaagaaaa agctcgccaa
    16201 aaggctgaga aagaggctag attagaacaa gaagagactg aaaaagcctt actcgatttg
    16261 cctcctgttg atatggaaac gggtgaaatt ctgacagagg aagctgttca aaatcttcca
    16321 cctattccag aagaaaagtg ggtggaacca gaaatcatcc tgcctcaagc tgaacttaaa
    16381 ttccctgaac aggaagatga ctcagatgac gaagatgttc aggtcgattt ttcagccaaa
    16441 gaagcccttg aatacaaact tccaagctta caactctttg caccagataa accaaaagat
    16501 cagtctaaag agaagaaaat tgtcagagaa aatatcaaaa tcttagaagc aacctttgct
    16561 agctttggta ttaaggtaac agttgaacgg gccgaaattg ggccatcagt gaccaagtat
    16621 gaagtcaagc cggctgttgg tgtaagggtc aaccgcattt ccaatctatc agatgacctc
    16681 gctctagcct tggctgccaa agatgtccgg attgaagcac caatccctgg gaaatcccta
    16741 atcggaattg aagtgcccaa ctccgatatt gccactgtat ctttccgaga actatgggaa
    16801 caatcgcaaa cgaaagcaga aaatttcttg gaaattcctt tagggaaggc tgttaatgga
    16861 accgcaagag cttttgacct ttctaaaatg ccccacttgc tagttgcagg ttcaacgggt
    16921 tcagggaagt cagtagcagt taacggcatt attgctagca ttctcatgaa ggcgagacca
    16981 gatcaagtta aatttatgat ggtcgatccc aagatggttg agttatctgt ttacaatgat
    17041 attccccacc tcttgattcc agtcgtgacc aatccacgta aagccagcaa ggctctgcaa
    17101 aaggttgtgg atgaaatgga aaaccgttat gaactctttg ccaaggtggg agttcggaat
    17161 attgcaggtt ttaatgccaa ggtagaagag ttcaattccc agtctgagta caagcaaatt
    17221 ccgctaccat tcattgtcgt gattgtggat gagttggctg acctcatgat ggtggccagc
    17281 aaggaagtgg aagatgctat catccgtctt gggcagaagg cgcgtgctgc aggtatccac
    17341 atgattcttg caactcagcg tccatctgtt gatgtcatct ctggtttgat taaggccaat
    17401 gttccatctc gtgtagcatt tgcggtttca tcaggaacag actcccgtac gattttggat
    17461 gaaaatggag cagaaaaact tcttggtcga ggagacatgc tctttaaacc gattgatgaa
    17521 aatcatccag ttcgtctcca aggctccttt atctcggatg acgatgttga gcgcattgtg
    17581 aacttcatca aggctcaggc agatgcagac tacgatgaga gttttgatcc aggtgaggtt
    17641 tctgaaaatg aaggagaatt ttcggatgga gatgctggtg gtgatccgct ttttgaagaa
    17701 gctaagtctt tggttatcga aacacagaaa gccagtgcgt ctatgattca gcgtcgttta
    17761 tcagttggat ttaaccgtgc gacccgtctc atggaagaac tggagatagc aggtgtcatc
    17821 ggtccagctg aaggtaccaa acctcgaaaa gtgttacaac aataaaaaaa tagcttcttt
    17881 ccgagtttgg agggaagcta ttttagtggc tattgattgc ttttattttc tgaagttggc
    17941 gcattggact gtttttcgtt ttcagtagca ggtttacttg aagcaggagt agaagagtcc
    18001 tgagttgctg ttttctgatc ttcttttttc tcttccttga cgctagattt tggtgtttcc
    18061 tcttgctgtg ttttttcttg actagtgtta gtctctttag ttggactggt gttttcctta
    18121 ggggattcct tttggatttc tttgacaatg gttgtcgtct ggcttgtcgt aggttctttt
    18181 ttaatatttt tgttattatc caaggcgttc atgatcgtga tacccgcggt aatcaagcca
    18241 aggatactag cgattgtagc aacggttttt tgaaagactg taaagccttt tttgatgtgt
    18301 tctgtttttg gttttgaagt tctacgatag ttagacatac attctctcct ttgattatga
    18361 tttattatac tctgtttctt taaaaaaatc ttaaaaaaag agaaattgcc ctttcttgtc
    18421 gcaggctccg tttcatgata caatagaagg agtgatttta gaaagcgtga gaaacatgat
    18481 ttactttgat aattcggcga cgaccaggcc ctatccagaa gcccttgaaa cctatatgca
    18541 ggtggcttca aaaattttag gaaatccatc tagtctccat cgtttgggag accaggcaac
    18601 acgaatttta gatgcttccc gtcaacagat tgcagattta atcggtaaga aaagtgatga
    18661 aatcttcttt acatctggtg ggacagaagg ggataactgg atcatcaagg gagtagcatt
    18721 tgaaaaagcc cagtttggca agcacatcat cgtatcagcc attgaacatc cagcagttaa
    18781 ggaatcagcc ctctggttga aagctcaagg ttttgaagtg gattttgctc cagttgataa
    18841 caaaggattt gtggatgttg aggcgctaga agatttgata agacctgata cgaccctcgt
    18901 ttccatcatg gcagttaaca atgaaattgg ctctgttcag cccattgagg ctatttcaaa
    18961 actattggca gacaagccaa ctatttcctt ccacgttgat gcggttcagg cgcttgccaa
    19021 gattccgact gaaaagtatc tgacagaacg ggtggattgc gcgaccttct cgagtcataa
    19081 atttcatggt gtccgtggtg ttggctttgt ctatatcaaa tctggcaaga agattactcc
    19141 tcttttaaca ggtggtggtc aggaacgtga ttaccgttcg acaactgaaa atgtggcagg
    19201 aattgcagcg acagccaagg ccctccgttt atctatggaa aagctagata tctttaggag
    19261 caagactggg cagatgaagg cagtgattcg tcaagcactt ctggactatc cggatatatt
    19321 tgtcttttca gatgaggaag acttcgcccc tcatattctg acttttggaa tcaagggtgt
    19381 tcgtggtgag gtcattgttc acgcctttga agactatgat attttcatct caacgacctc
    19441 ggcttgttcg tccaaggctg ggaaaccagc cggaaccctg attgctatgg gagtggacaa
    19501 ggataaggct cagtcagctg tgcgtcttag tctagacctt gaaaatgata tgagtcaggt
    19561 cgagcagttc ttgaccaagc taaaattaat ttacaatcaa actagaaaag taagatagga
    19621 gcattcatgc agtattcaga aattatgatt cgctacggtg agctgtcaac caagggcaaa
    19681 aatcgtatgc gtttcatcaa taaacttcgc aataatattt cagacgtttt gtctatctat
    19741 ccccaagtta aggtaacagc agatcgcgac cgtgcccacg cttacctcaa tggagcagat
    19801 tacacagcag tagcagaatc gctcaaacaa gtatttggaa ttcaaaactt ttcccctgtt
    19861 tataaggttg aaaaatctgt agaagttctg aagtctgctg tccaagagat tatgcaggac
    19921 atctacaagg aaggcatgac ctttaaaatc tctagcaagc gtagcgacca cacctttgaa
    19981 ttggacagtc gtgaactcaa ccaaacgctt ggaggggctg tattcgaagc cattccaaac
    20041 gtacaagctc aaatgaaaag tcctgacatc aatcttcagg tggagatacg tgaagaagca
    20101 gcctatcttt cttatgaaac cgttcgaggg gcaggtggat taccagtggg aacttctggt
    20161 aagggcatgc tcatgttgtc agggggaatc gattcccctg tagctggcta tctagcgctt
    20221 aaacgagggg tagatataga ggcagttcac tttgccagcc caccttacac gagtcctggt
    20281 gctcttaaaa aagcccaaga tttgacccgt aaattaacca aatttggggg aaatatccag
    20341 tttatcgaag ttccttttac agagattcaa gaggaaatca aagccaaagc gccagaagcc
    20401 taccttatga ccttgacgcg tcgttttatg atgcgtatta ctgaccgtat tcgtgaggta
    20461 cgaaatggtt tagttatcat caatggggaa agtctaggtc aagtagccag ccaaaccctt
    20521 gaaagtatga aggctatcaa tgctgttacc aacactccca tcattcgtcc tgtggttacc
    20581 atggacaagt tggaaatcat tgacatcgcc caggaaatcg atacctttga catttcaatc
    20641 caaccgtttg aagactgttg taccattttt gcaccagatc gtccaaaaac aaatcctaaa
    20701 attaagaatg cggagcagta cgaagcgcgt atggatgttg aaggcttggt tgagcgagca
    20761 gtggctggaa tcatgattac tgaaatcaca cctcaagccg aaaaagatga agttgatgac
    20821 ttgattgaca atctgctcta attcagaaaa tccaaaagaa tagcgaaaat cagtaaaaaa
    20881 agttagtttt ttctctaaaa acaggtaaaa aactaacttt ttttattttt atgatataat
    20941 gatataaaat tttgaatata gagagttttc tgacaatgaa tcaatcctac ttttatctaa
    21001 aaatgaaaga acacaaactc aaggttcctt atacaggtaa ggagcgccgt gtacgtattc
    21061 ttcttcctaa agattatgag aaagatacag accgttccta tcctgttgta tactttcatg
    21121 acgggcaaaa tgtttttaat agcaaagagt ctttcattgg acattcatgg aagattatcc
    21181 cagctatcaa acgaaatccg gatatcagtc gcatgattgt cgttgctatt gacaatgatg
    21241 gtatggggcg gatgaatgag tatgcggctt ggaagttcca agaatctcct atcccagggc
    21301 agcagtttgg tggtaagggt gtggagtatg ctgagtttgt catggaggtg gtcaagcctt
    21361 ttatcgatga gacctatcgt acaaaatcag actgccagca tacggctatg attggttcct
    21421 cactaggagg caatattacc cagtttatcg gtttggaata ccaagacaaa attggttgct
    21481 tgggcgtttt ttcatctgca aactggctcc accaaccgct atatcgagtg ccagaaacta
    21541 tcgcctgacc aacgcatctt catctatgta ggaacagaag aagcagatga tacagacaag
    21601 accttgatgg atggcaatat caaacaagcc tatatcgact cgtcgctttg ctattaccat
    21661 gatttgatag cagggggagt acatctggat aatcttgtgc taaaagttca gtctggtgcc
    21721 atccatagtg aaatcccttg gtcagaaaat ctaccagatt gtctgagatt ttttgcagaa
    21781 aaatggtaag ttaagaaagg aaaaaacgaa atgcatattg aacatcttag ccactggagt
    21841 ggtcatccta accgtgaaat gtaccttaac cgttatggac atggtgggat tccagttgtg
    21901 gtctttgctt catcaggtgg tagtcacaac gaatactatg attttggcat gattgatgcc
    21961 tgtgcttcct ttatcgagga aggccgtgtc cagttcttta ccctatctag tgtggatagt
    22021 gagagctggt tggctacttg gaaaaatgct catgaccaag cggaaatgca ccgtgcctac
    22081 gaacgttatg tgattgagga ggccattctt ttatcaagca caagacaggt tggtttgatg
    22141 gcatgatgac gacaggttgc tctatgggag cctatcatgc actcaatttc ttcctccagc
    22201 atccagatgt ctttaccaaa gtgattgctc tcagtggtgt ttacgacgca cgtttctttg
    22261 tcggtgatta ctacaacgat gatgctattt accaaaactc gccagtagat tatatttgga
    22321 actaaaacga cggctggttt attgaccgtt accgtcaggc agagattgtg ctgtgtacgg
    22381 ggcttggagc ctgggaacaa gatggtttgc catcctttta caagctcaaa gaagcctttg
    22441 acaagaaaca aattccagcc tggtttgctg aatggggaca tgatgtcgcc catgactggg
    22501 aatggtggcg taaacaaatg ccttatttcc tcggtaatct ctatttataa aaggagttac
    22561 ctatgaatta ccttgttatt tctccctact atccacaaaa ctttcaacag tttaccatcg
    22621 aactagctaa taaaggcatc acagtcttgg gaattggtca agagtcttac gagcaattgg
    22681 atgagccctt gcgcaatagc ttgaccgagt attttcgtgt tgataatctt gagaacatag
    22741 atgaagtcaa acgtgcagtt gcttttctct tttataaaca tggtccaatt ggccgcatcg
    22801 agtctcacaa tgaatactgg cttgagctag acgcaacact cagagaacaa ttcaatgttt
    22861 ttggtgccaa accagaggat ctcaaaaaga cgaaatataa gtctgaaatg aagaaacttt
    22921 tcaaaaaagc aggtgttcct gtggtacctg gagctgttat caagacggaa gcagatgttg
    22981 atcaagcagt gaaagaaatc ggtcttccaa tgattgccaa acctgataat gaagtgggag
    23041 cagccgcaac ctttaaactt gagacagaag acgatatcaa tcacttcaag caagaatggg
    23101 accattcaac cctttatttc tttgaaaaat ttgtcacttc cagcgaaatc tgtacctttg
    23161 acgggctcgt ggacaaggat ggaaagattg tcttctcaac aacctttgac tacgcctata
    23221 caccgcttga cctcatgatt tataagatgg acaattctta ttatgtgctc aaggatatgg
    23281 atcctaaact gcgcaagtat ggggaagcaa ttgtcaaaga atttggtatg aaagaacggt
    23341 ttttccatat tgagttcttc cgtgaggggg acgattatat taccattgag tacaataacc
    23401 gccctgcagg tggtttcacc attgatgttt ataactttgc tcattccttg gacctctatc
    23461 gtggatatgc agccattgtc gcaggagaag agttcccagc gtcagacttt gaacctcagt
    23521 attgtttggc tacatcacgt cgtgcaaatg ctcactatgt ctattctgag gagaacttac
    23581 ttgccaaata tagccagcag ttcaaggtta aaaaaatcat gccagaggct tttgcggaac
    23641 ttcaaggaga ttacttttat atgctgacaa ctccgagtcg acaagaaatg gatcagatga
    23701 ttgctgattt cggacaacgt caagaataag aactatcgga ttaaggaaat tcactccctt
    23761 aatccttttg ttttgtctgg taaaaaataa gagcatccta acaaggtagc tatcataaaa
    23821 cttgttcgat aactatttga agcaggatta ggttgctcaa aacactgttt tgaggttgtg
    23881 gatagaactg acagagtcag tatcatatac ctacggcaag gtgaagctga cgtggtttga
    23941 agagattttc gaagagtata aaatattcag gtgacgcata gatatagtta attgaagctt
    24001 tgtttgaaat ctgataaaat aatgatatta ctaagtttta aaaactaaag aaaagggaag
    24061 atatgattac aggcgaatta aaaaataaaa tcgatcagct gtgggaaatt ctttggacag
    24121 aaggaaacgc aaatccttta acaaatattg aacagttgac ttatctctta tttatgaaag
    24181 atttggatag tgtcgagctt ggacgtgaaa gtaatgctga atttctaggg attccttatg
    24241 agggagtttt tccaaaagat aaacctgaat accgttggtc aacttttaaa aatataggag
    24301 atgctcagga agtttatcgt ttaatgactc aggagatttt tccgtttatt aaaaatctca
    24361 agggggatac agatgataca gccttttcac gatatatgcg agaagctatt tttcaaataa
    24421 ataaacctgc tacgcttcaa aaggcaattt ctatcttaga tgtttttcca actaggggat
    24481 tagatgtaga ttttgataat gacaaacaaa gtattactga tatcggagat atctatgaat
    24541 atctgttatc aaaattgtcg accgcaggta aaaatggaca gttccgtaca cctcgtcaca
    24601 tcatcgatat gatggttgag ttgatgcaac cgactatcaa agatatcatc tcagatcccg
    24661 ctatgggttc tgctggcttc ttagtatctg ctagccgtta cttaaagcgt aagaaagatg
    24721 aatgggaaac caatacagat aatatcaatc attttcataa tcagatgttt catggaaatg
    24781 atacggatac gactatgttg agacttgggg cgatgaacat gatgctacat ggagtagaaa
    24841 atccacaaat cagttacctt gactcgctgt ctcaagataa tgaagaagct gataaatata
    24901 ctttggtttt agcaaatcct ccttttaagg gctcacttga ctacaattca acctctaatg
    24961 accttcttgc aaccgtaaaa accaaaaaaa cagaattact ctttctttct cttttcttgc
    25021 gaactttaaa accaggtgga cgagcagcag ttatcgtacc tgatggtgtc ctttttggtt
    25081 cgtctaaagc tcataaagga attcgtcagg aaattgtaga gaatcataag cttgatgctg
    25141 taatctcaat gcctagtggt gtgttcaagc cttatgctgg agtttcaact gccattctca
    25201 tctttacaaa aactggtaat ggtggtactg acaaagtctg gttttacgat atgaaagcgg
    25261 atggtttaag tttggatgat aagcgacaac cgattagaga caatgatatt ccagatatta
    25321 tcgaacgctt tcatcatctt gaaaaagaag cagaacgtca gagaacggat caatctttct
    25381 ttgttccagt tgctgagata aaggaaaatg attatgattt gtctatcaat aaatataaag
    25441 agattgagta tgaaaaagtt gagtatgaac caacagaagt catattaaag aaaatcaatg
    25501 atttagaaaa agaaattcaa gctggcttgg ctgaattgga aaaattactc aagtagggag
    25561 gtggctgtat gaaaaaagtg aagttggggg aagtcttatc tctaaaaaaa ggcaagaaag
    25621 ccactgtact tgctgaacaa acaactctaa gccaacgtta tattcaaata gatgatttaa
    25681 gaaataataa taatttaaaa ttcactgaaa gtttaaatat gactgaagca cttccagatg
    25741 atattctaat agcatgggat ggtgctaatg caggaacagt tggttatgga ttatcgggag
    25801 ctgttggtag tacaattacg gtcttaaaaa agaatgagcg atacaaagaa aaaattatat
    25861 cagattactt gggagtcttt ttggaaagta aatcgcagta tttacgagat cattcaacag
    25921 gtgcaactat tcctcattta aacaagaata tattacttga tttacaatta gaattgctag
    25981 gtatcgaaga acaagagaac attatctgta ttcttaatac gattaaaagg cttattacta
    26041 aaagaaaatt tcagttagat gaactaaact tgctcgtcaa atcccgattt aacgagatgt
    26101 ttggggaaaa taaaatattt gaaagcattg ataacttatt tgatattata gatggtgata
    26161 ggggcaaaaa ttatcctaaa tcagatgagt tgtttagtga ggagtactgt ttatttttaa
    26221 atacaaagaa tgttactaaa aacggatttt cattcgatac aaagcaattt atcactaaaa
    26281 caaaggataa attacttcga aaaggcaaac ttgagcgtta tgatatagtc ttgacaacaa
    26341 gaggtactgt tggaaatgta gcgtactacg atgaattaat aaaatataaa catttacgta
    26401 taaattcagg tatggtaata ttacgtccca agacaccaaa tcacaatttg cttgtgagat
    26461 agctataaaa gtgtggagaa atagcttgaa atttagtata atatagctaa actatttgtt
    26521 taaagtgaga aaaaaatggg aaattttagc tttcttttaa aaaatgacga atatgaatct
    26581 ttttcaaaac cttgcattga agctgagaat atgattgcta catcaactgt ggctactgcc
    26641 tttatggcgc gtcgtgcttt agagcaggct gtccattgga tatatagtca cgattcatat
    26701 ttagaagctc cctatcgtgc tactctatct tctttagtat gggatgatga ttttagggat
    26761 atcgtagatt ctgaactcca caagcagata gttctgttga ttcggtgggg aaaccatgct
    26821 gctcatggtg gtgaaattaa ggaacgagaa gcgattttag ctttgcatca tttgtatcag
    26881 tttgttaatt ttatcgatta ttgttacagc aatgagtttg tggagcgtta ttttgatgag
    26941 aagtgcttac cactttcagc aaacatcaaa taccgagaaa ctccacaatc tatgataaag
    27001 ttacaagaca gtttaccaga actgcctgat tttcatgaac agatggctgc tcagtccgta
    27061 gaagttcaag agacttatac tgaaaaacgt gagactgcag cgcaacggca agatgtgcct
    27121 ttccatattg atcaattatc tgaggcagag acaagaaagc tctttattga tatcgatctc
    27181 cgtttagcag gatggatatt tgaagaaaac tgtcgtgttg agatagccgt tgatggtctc
    27241 aagcacggtt caggaattgg ttactgtgac tatgtacttt atggtaaaaa tgggaaaatt
    27301 ttagcgattg tggaggctaa aaaagcctct gtcaatccag aagtagggga agtacaggtc
    27361 aaagaatatg ctgaagcttt ggagaaacat atcggctatc agccaatttg ctttattaca
    27421 aatgggttga agcactatat acttgatggt ccgaaccgcc gccagattgc aggcttttac
    27481 tctcaagaag aattgcaatt agtgatggat agacgtcatc ttcaaaaacc gcttgaggat
    27541 atttctagta aaattaggga cgatatttcc gggcgtcact accaaaaaca tgccattgca
    27601 agcgtttgtg aagctttctc taatcatcgt agacaggcac ttttggttat ggcaactggg
    27661 gctgggaaaa ctcgtacagc agtttctcta gttgatatct tatcacgtca taactgggta
    27721 aaaaacgttc tcttcttagc cgatagaact tccttggtta agcaagcata tgattcgttt
    27781 agaaaattac tcccagatct ttccgtttgt aacttcttag aagataaaga aggagctcaa
    27841 tcaagtcgca tggtcttttc aacttatccg accatgattg gagcgattag tggtcaagaa
    27901 gaagtaaatc aacgcccttt cactgttggg cattttgacc ttatcataat tgacgaatct
    27961 caccgttcta tttatcagaa atacaagtcc atttttgatt attttgatgc aagaattgta
    28021 ggcttaacag ctactccgcg tcaagattta gataaaaaca cctatggatt ctttaatttg
    28081 ggagaatggg gttccaacat atgcatatga tttggaagag gctgttaaag acggatattt
    28141 agtagcctat cattctatcg aaaccaaact gaaactacct acggatggtc tacattatga
    28201 tgatttgtcc gaagaagaaa aggaacattt tgatagcaaa tttgaagacg atagctgtga
    28261 aaaagatatt gatgggagtg tatttaattc ctttattttc aataaaagta cagtagaaat
    28321 tgttttaaat gaactcatga caagaggaat tcagacagcc tcgggtgatg aaattggtaa
    28381 aactattatt tttgctaaaa atcatgatca tgcggaatat atcagaggta tttttaacaa
    28441 ccgctatcct gaaaaaggga gcgactatgc tcaggtgatt gattatagta ttaagcatta
    28501 tcagaccttg attgatgatt ttaaaattaa ggagaagtat cctcaaattg cgatttctgt
    28561 cgatatgtta gatacaggta ttgatgtacc agaggttgtt aatttagtct tcttcaagaa
    28621 agtacgctct aaaactaagt tttggcagat gattggtcga ggaacccgtc tatgtaaaga
    28681 tttatttgga cctgagcagg ataaggaaaa cttcttggta tttgattatg gggacaattt
    28741 tgattatttt cgtgcagatc caagagatgg agagggtcgt cacattgttt cgctgactca
    28801 gcgtttattt aatatcaaag tggacttgat tcgagaactt cagggactcc aataccaaga
    28861 agatcagttt gcgagagcat accgtcagca gcttgtctcg gaacttcaag gtcgtataga
    28921 gagcttaaat gagttggact tcagggttcg tatggtttta gatacagttt atagctatag
    28981 gaaattggaa agttggcaga atctaactgc tgttacaagt gaaaccattc aaaaaaatct
    29041 ctctccgctt ttatttgatg aagataaaga agatgagatg gcgaggagat ttgatttgtg
    29101 gttgcttcat attcagttgg ggcaactgac agctaaatct tccactgttc atatttccca
    29161 agtgatgaag acggctagag ctctttctgc tattggcaat atcccgcagg tttttgagca
    29221 ggctgaaatt atcaggaaag tacaggagcc tgaattttgg aaagaagtta acttgtctga
    29281 tttggaaaaa attcgtcttg ctattcgaga tttattacag tttttggata aaacagaccg
    29341 taaaccctac tatgttaact ttgaagatcg tatactctcc actgttcacg agaccacagc
    29401 atttttgcag gtcaacgatc ttcggtctta caatgaaaaa gttgagcatt atttgaaaac
    29461 tcatctggat gaggagtcca tttctaagct ataccataat aaaaagttga catctgatga
    29521 tatgcttgca cttgaaaaat tgctttggga aaaattaggt agtaaagcag actaccaaag
    29581 tcattatgaa aataaggcaa ttccgagatt ggttcgtgag attattggct tagatagaga
    29641 gtctgccaat cgtatttttt ctaaattttt gtcggatgag aatcttaatg ccaggcagat
    29701 ttcatttgta aaattgattg tagactacat tgtagaaaat ggttttttag agacgaaagt
    29761 gttaacgcaa gagccgttta aatcttatgg ttctgttcaa ctactcttcc aacaccaact
    29821 accagtactt cgtaatattg ttcaaatcat tgaacttatc aataatcgag ctggagaagc
    29881 ggcttaaatt ctaaagtgat tgccatgctg agactcattt aaaattaaaa agagtagaaa
    29941 tttatgctat atatgagaag ttttattagg aagaatgtca tcgttttcct agaatacagt
    30001 atcagttgtt aagtggttga taaatttcaa agtagatact tgtaccacga tgtttgttga
    30061 tcgagttatt aacaaaagag ctactttgat tttaaagaaa tagaaaacaa aaagccgagc
    30121 aagaattcaa ttgcaggaga aaatgaaata atactcaatg aaaatcaaag agcaaactag
    30181 gaaattagct gcaggctgct caaaacactg ttttgaggtt gtagataaga ctgacgaagt
    30241 cagctcaaaa catggttttg aggttgtaga tgaaactgac gaagtcagta accataccta
    30301 cggcaaggcg acgttgacgc ggattgaaga gattttcgaa gagtataaat cttcctagga
    30361 taaagcaaaa cgcatagtat caagtgttga aaacccttga tactatgcgt tttctgatgt
    30421 taaagacttt ctaccagttt ttttaagcat aattgttagt tgtagtcatt tattattctt
    30481 caaagaaaaa tggtggggcg aattttttca gttcttcaaa gcacttttga gcagtatctg
    30541 catcttcaca gatgataaga cagacatcat taccacaaag ggtagcgata gcgtcaggga
    30601 agctcaaagt atcaatgata gaaccaaagg attgagccag tccaggaagg gtttttagta
    30661 ggacttggtg ttgaactggg cgcatccaga caagggcgtc ttccatgtag agttcgagac
    30721 gtttttccca ttttgagatg gaaccattgt taagaacata ataagcgcta tcttcttcgc
    30781 ggacttttga taggttcata tttttgatgt cgcgtgagag ggttgcctgg gttacttgaa
    30841 tgtcgttctt agcaagaagg gcttgcaact cagcctgtgt atgaatcttg ttttttgtga
    30901 taagagcgcg tataagttgg tggcggtgtt ctgatttatt cataataatg taactccttt
    30961 tagcaaggta aggtaagcat ggactgagcg aggtcgacag tcaagtggta gtctgtattg
    31021 tcacggatgg tgatttcaaa gtcagtagta tagaggacta aacggagagt gtctccttct
    31081 tttagcttgt aaatagttgg ctgcagttca aattgaacgt ccatccattc atctgcagta
    31141 atatcctcta ctaacagtaa atcatttcta ttttgtaaat taaggtaacc ttttgtcacg
    31201 actcgttgtg cctctggtct aaatggcaat tcacagagat tttccaacat gtgatagcga
    31261 ccgttgtcaa tggttctagc acttaaaata gctggataag gttgtaggta tttcttttgc
    31321 ccaaattcta gcagttgggc agataagagc cccttgtttg tactggattt gatacgaaga
    31381 ttgagctgag cgcgaccgtt taggtggaga tctttagtca caggaaggtt aatagtaatc
    31441 tgattagctt tcccttgata gagctctgta ttgaaggttt ggtatgtctt actatagcgc
    31501 tcaaaatcct tatctgggta ctggttttga atagcttgct cttcttgacc aagtgagaag
    31561 gttttacagt tttcttgccc accgaagtta tcaagtgata accaagtctg tggagctgta
    31621 ttgtcctgcc agataacagt aggaagttga aagtctgttt cctgtcctag taatttcttg
    31681 gttaataagg catttatgga ctcacggaag tcaattgatt gccaattgtt catgtaaaca
    31741 tgggcaccat tatggaaaaa gagatgcttg tgtatatgag taggaagagc atggaacatc
    31801 tggtaaacat gaagtggttt gacattccaa tcctgagaac catgagtaaa gacaacctct
    31861 gcctttactt tatgggcatt gagcagataa ttgcggtcat gccaaaactg attgtagtcc
    31921 ccagtttttc ggtctagctg agctttcact ttttctaagt cagcttggtg agcttcattg
    31981 ccacggatat agtcgccagc taagagatta cgagaatagg ttaactcagc aagggagtca
    32041 aagtcctcac ctggataacc acctgggcta gtcaccagac cgttttcacg gtagtagttg
    32101 taccatgatg aaattcctgc ctcggcaatg ataacttcta aaccatcgac tcctgtagtc
    32161 gcaagaccat tggacatggt acctagatag gaaagtcccg ttgtagcaac ttttccgttt
    32221 gaccaatcag ccttgacttg acgctggcgc gtgtgatcag taaaggcatg gcaacggccg
    32281 ttaagccaat cgatgacatt tttataagcc tcgatttgct ggtagtctcc attagtcatg
    32341 aaacctgtcg agtctttggt accaacacct gagacataga gattggcaaa gcctctcgga
    32401 aggaagtagt cgtttagtgt atagctagag ttgatgtgag ttagcttttc ctcagcctct
    32461 gctataagct cagcttgacc ttggggttgg acgagattta gttgaggttt ctctagctca
    32521 atcttgcgag gaagcttaac cttaagctcg ccctccatct tgtagagagc cttgtcacta
    32581 gccttgtcat tggttccctg atgataaggg ctggctgtca tgatggcagg gatttttcca
    32641 tcaaaacgag ggcgaataat gctaaccttt actaggtctg atagcccttt ttggtcagta
    32701 tcgacacgag actcaacgta aacgacttca cgaatgacat cctggttaga aaaagtagcc
    32761 aaactcttgc cgttaaagta gtggtagtca ttatcctccg gaataagacc atcactaaca
    32821 agttggtcga taagagtatt tccttttttg gtgcgagtat tgagtaactg atagagattt
    32881 tcaatcaagt caccatatat aatgggaaat ccagtttctt tacgaaaaac gtcactatct
    32941 tcgaagtcaa ccaaataaga aaagcctaaa agttgaaaag caacagtata aaaaatatct
    33001 gctgtcagtt catcttctga ttgaaaaaat gtcagcaggt ctgttttttt atcagctgct
    33061 aggatagaaa gtgggtagtt ggtgtcttga taagtgaaaa agaaacgacg taaaaaggtt
    33121 tcaagtgagt ctttgtgatt ggctgtattt tgtaaatcaa agccacattt ttttagctca
    33181 gataagacat tttcttttgg aaaattgata taactatatt gattaaaacg catagaacct
    33241 ccatatagaa tgacagttaa ggttattata tcaaaaaaaa agcagaaagg gaattgttaa
    33301 cttcaaaagg aaataatcca at
//
LOCUS       DAVKWZ010000019        33298 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000019 DAVKWZ010000000
VERSION     DAVKWZ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33298
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.019"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            1..1037
                     /locus_tag="VW854_000932"
                     /pseudo
     CDS             1..1037
                     /locus_tag="VW854_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
     gene            1432..2276
                     /locus_tag="VW854_000933"
                     /pseudo
     CDS             1432..2276
                     /locus_tag="VW854_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
     gene            2562..2795
                     /locus_tag="VW854_000934"
     CDS             2562..2795
                     /locus_tag="VW854_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262189.1"
                     /translation="MLNLQFAETMELTEAELQDVRGGNLVNSMGGGGRSGISGWGVPG
                     IYPGWGNQGMSPNRGAFDWTIDLADGLFGRRRR"
     gene            2817..3494
                     /locus_tag="VW854_000935"
     CDS             2817..3494
                     /locus_tag="VW854_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0262190.1"
                     /translation="MKKMKEVKFHLATGLLILTYYLIFNVTSDLDFMVALSDNMYYVF
                     QVLLVLILGTIATIAFVKSEHWKECGRFQFRWSYLGVFLLSFFLLFVWANLTTYIFPR
                     TQNGSTVVEVATNLTGISYFVTRILYTSIIAPVSEEVVCRGLLMTSLSKVKRYYLDVL
                     VSAAIFGAMHVLQYGWITTDFIKYFGMGLIFCMMFRYTRSIYWAIALHASWNSFLLIV
                     TLLVFGY"
     gene            3506..4171
                     /locus_tag="VW854_000936"
     CDS             3506..4171
                     /locus_tag="VW854_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0262191.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVAGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVAWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEDMIYRGLVMTALEKGKKWGLDVL
                     GSAVLFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            4243..5469
                     /locus_tag="VW854_000937"
     CDS             4243..5469
                     /locus_tag="VW854_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV0262192.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQILQIFLGVLADFQHHRVLKYTVISFAKFLLYSIVSLSLSGQSFSLL
                     LVAFICLLNLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPISTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            complement(5821..5898)
                     /locus_tag="VW854_000938"
                     /pseudo
     CDS             complement(5821..5898)
                     /locus_tag="VW854_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            5969..6625
                     /locus_tag="VW854_000939"
     CDS             5969..6625
                     /locus_tag="VW854_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEV0262193.1"
                     /translation="MPTALGYVSIGLACGIIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTVLGDLLPNSEIFGLDFALVGMFIGI
                     FASQFQIMQRRIPVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGVVLDGQ
                     "
     gene            6615..6938
                     /locus_tag="VW854_000940"
     CDS             6615..6938
                     /locus_tag="VW854_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEV0262194.1"
                     /translation="MVSKYLLLAVIFSGLVTWILRMIPFILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAI
                     LRLVF"
     gene            7042..7872
                     /locus_tag="VW854_000941"
     CDS             7042..7872
                     /locus_tag="VW854_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV0262195.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGEAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            8026..8880
                     /locus_tag="VW854_000942"
     CDS             8026..8880
                     /locus_tag="VW854_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV0262196.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLKNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAIPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEETS
                     DGLDQPVW"
     gene            8980..10353
                     /locus_tag="VW854_000943"
     CDS             8980..10353
                     /locus_tag="VW854_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV0262197.1"
                     /translation="MVFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREIALLETYGQRNPEE
                     VSRIYGLELPLLQEDRMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            10346..11407
                     /locus_tag="VW854_000944"
     CDS             10346..11407
                     /locus_tag="VW854_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEV0262198.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGGQ"
     gene            11409..12101
                     /locus_tag="VW854_000945"
     CDS             11409..12101
                     /locus_tag="VW854_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0262199.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIVIILIIFAIQS
                     LGDFLTKKLSHK"
     gene            complement(12131..12700)
                     /locus_tag="VW854_000946"
     CDS             complement(12131..12700)
                     /locus_tag="VW854_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEV0262200.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIAFFFLGTKHGAGSFLPGIICGLLADEVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            12835..13449
                     /locus_tag="VW854_000947"
     CDS             12835..13449
                     /locus_tag="VW854_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEV0262201.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFTLSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            13451..15097
                     /locus_tag="VW854_000948"
     CDS             13451..15097
                     /locus_tag="VW854_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV0262202.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDR
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQRYFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            15109..16395
                     /locus_tag="VW854_000949"
     CDS             15109..16395
                     /locus_tag="VW854_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0262203.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLTSFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            16447..16980
                     /locus_tag="VW854_000950"
     CDS             16447..16980
                     /locus_tag="VW854_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0262204.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELA"
     gene            17058..17528
                     /gene="nrdI"
                     /locus_tag="VW854_000951"
     CDS             17058..17528
                     /gene="nrdI"
                     /locus_tag="VW854_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEV0262205.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(17821..19083)
                     /locus_tag="VW854_000952"
     CDS             complement(17821..19083)
                     /locus_tag="VW854_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEV0262206.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
     gene            complement(19393..19851)
                     /locus_tag="VW854_000953"
     CDS             complement(19393..19851)
                     /locus_tag="VW854_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEV0262207.1"
                     /translation="MKKQVFHDAATGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWATIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(19848..20288)
                     /locus_tag="VW854_000954"
     CDS             complement(19848..20288)
                     /locus_tag="VW854_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0262208.1"
                     /translation="MKVELQISETYEEEKLIVQAPQETEKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPTNFVQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            20494..22443
                     /gene="mutL"
                     /locus_tag="VW854_000955"
     CDS             20494..22443
                     /gene="mutL"
                     /locus_tag="VW854_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEV0262209.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(22768..23235)
                     /locus_tag="VW854_000956"
     CDS             complement(22768..23235)
                     /locus_tag="VW854_000956"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEV0262210.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEISLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(23236..24441)
                     /locus_tag="VW854_000957"
     CDS             complement(23236..24441)
                     /locus_tag="VW854_000957"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEV0262211.1"
                     /translation="MEYRKIQETLEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRIHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(24461..25096)
                     /gene="ribE"
                     /locus_tag="VW854_000958"
     CDS             complement(24461..25096)
                     /gene="ribE"
                     /locus_tag="VW854_000958"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEV0262212.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSINGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLKQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(25081..26181)
                     /gene="ribD"
                     /locus_tag="VW854_000959"
     CDS             complement(25081..26181)
                     /gene="ribD"
                     /locus_tag="VW854_000959"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEV0262213.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(26316..26430)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            26587..27180
                     /gene="ruvA"
                     /locus_tag="VW854_000960"
     CDS             26587..27180
                     /gene="ruvA"
                     /locus_tag="VW854_000960"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEV0262214.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQED
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            27190..27753
                     /locus_tag="VW854_000961"
     CDS             27190..27753
                     /locus_tag="VW854_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEV0262215.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTETELEAMLENPAIIRNRAKLFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            27750..28427
                     /locus_tag="VW854_000962"
     CDS             27750..28427
                     /locus_tag="VW854_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0262216.1"
                     /translation="MMSNKNKEILIFAILYTVLFVFDGVKLLASLMPSAIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLPGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(28564..29595)
                     /locus_tag="VW854_000963"
     CDS             complement(28564..29595)
                     /locus_tag="VW854_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEV0262217.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGRAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKSEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVILFDYNK"
     gene            complement(29850..30533)
                     /locus_tag="VW854_000964"
     CDS             complement(29850..30533)
                     /locus_tag="VW854_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEV0262218.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(30545..31489)
                     /locus_tag="VW854_000965"
     CDS             complement(30545..31489)
                     /locus_tag="VW854_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV0262219.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            complement(31645..32811)
                     /locus_tag="VW854_000966"
     CDS             complement(31645..32811)
                     /locus_tag="VW854_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV0262220.1"
                     /translation="MKITEVIKKDGSKVYRANIYLGVDQVTGKKVKTKVTGRTQKEVK
                     QKATQEKIAFQKAGSTRQKASTIKNYQELANLWLESYKNTVKPNTQDNVRKLIDNHIL
                     PIFGAYKLDKLTTPLIQSIINELADKTNRGEKGAFLHYDKIHALNKRILQYGVTMQAI
                     PSNPARDVVLPRNTQKAKRKKVKHFENQELKKFLGYLDNLDSDRYRYYYETTLYKFLL
                     ATGCRINEALALSWSDIDLDNAVVHVTKTLNRDLEINSPKSKASYRDIDIDQATVSML
                     KQYKLRQTKEAWKIGQRESVVFSDFIHEYPSSSRLKRRLQTHFKRADVPNIGFHGFRH
                     THASLLLNSGIPYKELQYRLGHSTLSMTMDIYSHLSKENAKKAVSFYETALKAL"
BASE COUNT         9662 a         6167 c         7027 g        10442 t
ORIGIN      
        1 aaaatgaaga gcttatgaag tttttttgcg agaataactt aaatattact ttttttgata
       61 cattttctga aattaaaaat aatattgatt attatattat tgcgcttccg acagattatg
      121 atgagaaaat tggtagtttt aatacatatg aaatcgaaca aacggtattg aagattctga
      181 gggtaaaacc taatggaaag attatttgaa agtcaacagt tccgatcggc ttttcaaaca
      241 aattaaaaag gctgtttgat acaaaaaata tcatttttgt ccctgaattt ttgagagaag
      301 gttgttctat atatgataat ttatatccaa gtcgcatagt tgttggagat gagacagttg
      361 aaggaagaaa aattgcagag ttgttccttt cgattagtac tcatagtact gccaatatta
      421 aaaatgttat gttagtttct cctactgaag cagaagcaat taagcttttt tctaacacat
      481 tcttagctct ccgtgttgct ttttttaatg aactagattc ttttgctgag aggagaagtt
      541 taaatgctga agttgtaata aaaggtgttt gtttagatcc aagaattgga aatttttata
      601 ataatccttc ttttggattt ggaggatatt gtcttcccaa agatactaaa caattaaaaa
      661 aagaatttat agaaataaat gccccagtga tagaagcgat tgatatttca aacacaaata
      721 gaaaacagtt catagttaaa caaatattgg aacgaaagcc aaagatagta ggaatatata
      781 aattggggat gaaatataat tcagataatt acaaagagtc agctatttta agtataatta
      841 atgaactatt gattgtcggt ataaaaattt tagtatatga accaaattta aacgttagta
      901 tagataacgt aatttttgaa aagaattttg aactatttat aaaacaaagt gatctaattg
      961 ttgcaaatag atgggatagg ggccttgaag cttataaaga taaagtttac acaaggggga
     1021 tctggataag agattaacta ttgatattaa aaaggcaata atttataaaa tttgtacaaa
     1081 aaaatgttaa attttcacat tttcatagta tactgtttaa tagaaagtag gttctttgta
     1141 accgctccat tataaaatcc tttcactctt ccggtatact attaggaaaa acataactgg
     1201 aggatatttt gagctcacac ctcatcgttc cagtggctgt tccccaagct cgtgtactgc
     1261 ccaaaaaagt tagacattct atttagaacg aggattgagt tctgtattgt acaaggctcg
     1321 gtccttttag agtcagctta aggctggctt tttcaatcac caaagtgtca gaatgttttg
     1381 acaaatgaac acaaataatg atataatatg caaaagctag gaggtggtag gatgattgaa
     1441 aaaatggaac tgggggaatt ttacaaggaa ttgcgcttgg ctagaaagct caagcagtca
     1501 gatgtggctt gtgctggact aacagcttct cagttgtcca agtttgaact agggcagtct
     1561 atgctgtctg cggataagtt gattctagct atccaaggta taaatgtgac ctttgatgag
     1621 tttgggcaca agctcaacaa ctatcaagaa tctccacata tgcgaatcgc tttgcccatc
     1681 aggatatagc tgctttagag cagctattgg aggaagtcga tcaagaacag atggcgcaga
     1741 cctatcgtcg tttgaatgcc atcgtgatta aagatgccat ccattcacta aataaaagct
     1801 acccactagc agaggaggat agcgagtttt tgaccaccta tctctatgct atcgagtctt
     1861 ggacctggtt tgaactctat ctcttttgca ataccatgcc cttcttgagc aatcaagatc
     1921 tgattttttt atcaacctcc ttactcgaaa aatccaaaga atttaaagag ttagtacaca
     1981 atcgattgta tatgaagcaa ggactcttaa atatcttatc agaactcatg gagcgaaaac
     2041 ttttctccta catcccaatc ttcgaagccg agttggagag gatgttacga ccgtatgatg
     2101 tttttgaaaa agtatcgtgg caatttttaa agaagatgag tgtctttctt caaacaaagg
     2161 gaagcaatca aaaagagatt gaacgcttta tccaatctct gcaggtatta gaaaatccac
     2221 aattaacatc cctttttgaa ttgcgttttc agcaatacaa aaagcttatc gattagttag
     2281 aaatggcaaa ataataataa tctcagcaac gattttcaat aatgttaaaa tttgtttctt
     2341 catatttctc cactcctcat tagtagtaac tttattatac cagagaagta atcaaattca
     2401 ccaaaatcgc aaatctgaaa tttctataag aaaaaatatc aaatatgcga ttttctaaaa
     2461 taagctaatt ttcgtgttat actgtacttg taaagcactt gaagcaaatc ctaggtcgca
     2521 gaagtggttt acaaatgaag ataattgaag gagtgtaaaa gatgttaaat ttacaatttg
     2581 cagaaacaat ggaattgaca gaagctgagt tgcaagatgt tagaggaggc aacctcgtaa
     2641 attctatggg aggtggtgga agaagtggta tatctggttg gggcgtccca ggaatttacc
     2701 ctggttgggg caatcaaggg atgagtccta atcgtggagc ttttgattgg actatagatt
     2761 tagcagatgg attgtttggc cgccgtagaa gataggctct tataggagat aataggatga
     2821 agaaaatgaa agaagtgaaa tttcacctag caactggctt gttgattttg acttattacc
     2881 tcatttttaa tgtcacatca gacttagatt ttatggttgc tttgtctgat aatatgtact
     2941 atgtgttcca agttttattg gtacttattc tgggaactat agctacaatt gcttttgtca
     3001 aaagtgaaca ttggaaagag tgtggtcgtt tccagtttcg ttggtcttac ctgggtgttt
     3061 tccttctttc ttttttctta ttgttcgtgt gggcaaatct aacaacctat atctttcctc
     3121 gtacccagaa tggaagtaca gtcgtagaag tagccaccaa tttaacagga attagctatt
     3181 ttgttacgcg aatactttat actagtatta tcgcacctgt atcagaagag gttgtttgtc
     3241 gtggtctttt gatgacaagt ttatctaaag ttaagagata ctatcttgat gttcttgttt
     3301 ccgctgccat atttggcgct atgcatgttt tgcagtatgg ttggataacg acagatttca
     3361 taaagtattt tggaatgggc ttgatatttt gtatgatgtt tcgctacacc cgatccatct
     3421 attgggctat agctctgcat gcttcatgga atagtttctt actcattgtt actttattgg
     3481 tatttggata ttagaaaggt gatatatgaa aaagataatc tcacaccgct actttattat
     3541 agtttttcta ctagttattg ctgaccagaa gttcagtgtt ttagttttac gcagcgacct
     3601 tgttgctggg ctatctgact ttgcctatta tctgtcggat atgatgttga attttcttgt
     3661 ggttttattt gctcttattg ctatgatttg gtcggggaaa tggcagaaaa tcaatagtag
     3721 aaagtttaaa ggatcctatc ttttctattc cttcctagct ctgcttgctt ttgttgcttg
     3781 gaattttgtc accttttttc ttttcccacc tactcgaaat gaaatttctt atcaacatgc
     3841 tgctcctact tttacaggag ctacggcatt tttgatgtat tttttttatc ctgtgattgc
     3901 aggtcccatt tttgaagaca tgatttatcg tggattagtg atgactgctc tggaaaaagg
     3961 aaagaaatgg gggctagatg tacttggttc cgctgtttta tttggggtct cgcacattag
     4021 taatcacggt tgggtcttga cagactttgt cttctatatg ggtggaggtc ttatatttgc
     4081 agtcttattt agaatgacaa agtcaattta ttggcctatt ggactgcata tagtctacaa
     4141 tggcattggt cagcttttga tgttactgta attttggttg ttagtagtat cgcggtctat
     4201 ccttggggaa agattcccat ttgaacttaa gaaggagttg tgatgaaagt atttcttcaa
     4261 aatagagatt ttaggcaatt aactatcaac cagtggattt caacgcttgg ggatacgatt
     4321 ttttatctgg cctttctgaa ttatgtggca gatgcgtctt ttgcaccttt ggcgatttta
     4381 ctcattacga tttcagaaac ccttccccaa attttacaaa tctttctggg agttttggca
     4441 gattttcaac atcatcgtgt cttgaaatac acagtcatta gttttgcaaa atttttgctt
     4501 tactctatcg tttctttatc actttcaggg cagtctttct ccttgttgct agtagcattt
     4561 atttgtctgc ttaacctctt gtctgacaca ttgagttact tttcaggagc catgctcact
     4621 ccgattttca ttagaattat tgggcaagac catctggcag aagctattgg ctttaaacag
     4681 tcaactgtta gtttagtgaa aacaatcagt aatatcctag gaggagtctt actaggcatt
     4741 ctatccatcc agtttatttc cttactgaac gctcttacct ttttaatcgc atttttaggt
     4801 atcctcttca taaaaactga cctcttgaaa gtagaaaaaa cgattagcta tcaagaagga
     4861 ctctctgtaa aatccttttg ccagcatttg ctccaatcat caaaattgat atggaatatg
     4921 aataaggtgc tcttggtttt gtttattatc tctactagtc aagcagtgat aaatgttacg
     4981 gttcctattt ccactctatt tttaaggaac cagccctttt tgaatttaca aacaggtcaa
     5041 tctcttgcct tgctatccac ttttgaactg tcagccctga ttgtcggaag ccttgtaagt
     5101 ggctatctgc aacataccat ttccataaaa acagctctct atgcctcact tgtcatccag
     5161 ttgcttcttc tagtaggatt tgccacagtt cgttttgact ggattctcat ctttagtacc
     5221 ttggatgcct ttttcgcagg tgttctctct cctagattgc aagaactcgt ttttaaacaa
     5281 atacctgagg agtcaatggg agcagttcaa tcctctatcg gtgccattac ggttgtttta
     5341 cctagcttat ttacaatagc tttggtaacc attgctacta gctttggaac tctggcagtt
     5401 agctttgttt tattgctatt tcttctagtt gcctttgtta tgcttctgaa tattcgtgaa
     5461 agtatttagt ggagaatttc tataatatat gtttgagact ggattgccag tctttttatg
     5521 atggtcaaaa tcaacagact tttttctctt ttctgaatgt gttataatag actgaatcta
     5581 aaatagtacg aaacaattgc taaaacattt atagaaatta attttacttt cccaatcgat
     5641 ttgttctcat cttatttcaa tctgctatat aatagtccat gctgtggctg gattcttttc
     5701 agcctacttg tactcttcga aactctcttc aaaccacgtc agcttcactt tgccgtaggt
     5761 atggttactg acttcgtcag ttctatccac aacctcaaaa cagtgttttg agctgacttc
     5821 atcagttcta tctgcaacct caaaacagtg ttttgagcaa cctgcggcta gtttcctagt
     5881 ttgctctttg attttcattg agtattatta agacaatatg aggagaaaga tgaaagaaaa
     5941 aggattttag gagggggcgc aggcagccat gccaacggct cttggctatg tcagtatcgg
     6001 cctggcctgt ggaattatcg gtgcgcccta tgtgacacct gttgagatgg gcttgatgag
     6061 tctctttgtt tatgctggga gtgcccagtt tgccatgttg gcactgattg tggttcaagc
     6121 tcctgtggca gctattgcta tgacggtttt tctaatcaac ttgcgtctct ttttgttgag
     6181 tttacacgca tcgacttatt tccgtcatac cagtctttgg tacaatatcg gtatgtctag
     6241 tatcttgaca gatgagacct atggcgtttt gatgggtgaa ttggcccata cagacaaggt
     6301 aaatcctatg tggatgcacg gaaacaatct taacagctat gtggcttggt ttgtgggaac
     6361 agtggtcgga acggttctag gtgacctgct gccaaattca gaaatctttg gattggattt
     6421 tgccctggtt gggatgttca tcgggatttt tgcttcgcaa tttcagatta tgcaaagacg
     6481 gattcctgtc cgcaatctgc tcattatcct agcagttgtt gcggtgtcct tctttttgct
     6541 cttgacagtg gtgtctcagt cactagctgt tctgtttgcg acgctacttg gttgtagcat
     6601 gggggtggtt ttagatggtc agtaagtatc ttttattagc agttattttc tctggcttgg
     6661 tgacttggat tctccgtatg attcccttca tcttagtcaa gtataagggc ttgcctgcaa
     6721 tcgttgagcg ttttttgaag ttcttgcccg tttccattat ctttgccttg attctttcaa
     6781 gcgtagtgac aggtaaggtt gggagtcttc ctcaaattaa atggctagac ttcttagccg
     6841 tctttccaac agcttgggta gcctttcgct accgcaatct agtcggaaca gttctctttg
     6901 gagtggtctt gattgccatc ttgcgtttgg tcttttaaat tacccataaa aaaaacttat
     6961 cacagagata gatatcatat aatggcgtaa atgctctttt tctgttaaga ttataaggta
     7021 ttctattttg gaggaaatga catgaaaaaa atcgttaaat actcatctct tgctgcccta
     7081 ggacttgttg ctgcaggtgt gcttgcggct tgctcagggg gtgctaagaa agaaggagaa
     7141 gcagctagca agaaagaaat catcgttgca accaatggat caccaaggcc atttatctat
     7201 gaagaaaatg gcgaattgac tggttacgag attgaagtcg ttcgcgctat ctttaaagat
     7261 tctgacaaat atgatgtcaa gtttgaaaag acagaatggt caggtgtctt tgctggtctt
     7321 gacgctgacc gttacaatat ggctgtcaac aatcttagct acactaaaga acgtgcggag
     7381 aaatacctct atgccgcacc aattgcccaa aatcctaatg tccttgtcgt gaagaaagat
     7441 gactctagta tcaagtctct cgatgatatc ggtggaaaat cgacggaagt cgttcaagcc
     7501 actacatcag ctaagcagtt agaagcatac aatgctgaac acacggacaa cccaactatc
     7561 cttaactata ctaaggcaga cttccaacaa atcatggtac gtttgagcga tggacaattt
     7621 gactacaaga tttttgataa aatcggtgtt gaaacagtga tcaagaacca aggtttggac
     7681 aacttgaaag ttatcgaact tccaagcgac caacaaccgt acgtttaccc acttcttgct
     7741 caaggtcaag atgagttgaa atcatttgta gacaaacgca tcaaagaact ttataaagat
     7801 ggaactcttg aaaaattgtc taaacaattc ttcggagaca cttatctacc ggcagaagct
     7861 gatattaaat aatttcttga aatcatccat tctgagtaag gtgggtggtt tctcagtttt
     7921 tagggatata gttttaaact atatatctga ctatctaata acaatttgtg aaatcaaaca
     7981 aattgtgaga tactagtacg gtattatttt taaggagaaa gaatcatgaa aatcaaaaaa
     8041 tggcttggtc tagcagccct tgctacagtc gcaggtttgg ctcttgcagc ttgcggaaac
     8101 tcagaaaaga aagcagacaa tgcaacaact atcaaaatcg caactgttaa ccgtagcggt
     8161 tctgaagaaa aacgttggga caaaatccaa gaattggtta aaaaagacgg aattaccttg
     8221 gaatttacag agttcacaga ctactcacaa ccaaacaaag caactgctga tggcgaagta
     8281 gatttgaacg ctttccaaca ctataacttc ttgaaaaact ggaacaaaga aaacggaaaa
     8341 gaccttgtag cgattgcaga tacttacatc tctccaatcc gcctttactc aggtttgaat
     8401 ggaagtgcca acaagtacac taaagtagaa gacatcccag caaacggaga aatcgctata
     8461 ccgaatgacg ctacaaacga aagccgtgcg ctttatttgc ttcaatcagc tggcttgatt
     8521 aaattggatg tttctggaac tgctcttgca acagttgcca acatcaaaga aaatccaaag
     8581 aacttgaaaa tcactgaatt ggacgctagc caaacagctc gttcattgtc atcagttgac
     8641 gctgccgttg taaacaatac cttcgttaca gaagcaaaat tggactacaa gaaagcactt
     8701 ttcaaagaac aagctgatga aaactcaaaa caatggtaca acatcattgt tgcgaaaaaa
     8761 gattgggaaa catcacctaa ggctgatgct atcaagaaag taatcgcagc ttaccacaca
     8821 gatgacgtga aaaaagttat cgaagaaaca tcagacggtt tggatcaacc agtttggtaa
     8881 taagaaacag ggaggtggga gagagaattc cacctcttgc ttttgtatag agcacggagt
     8941 gtaaagaaga ctattcgttc atagaaaggt agagagaata tggtttttcc tagcgaacaa
     9001 gaacagattg aaaaatttga aaaggatcat gtagcccagc attattttga ggttttgcgt
     9061 accttgattt ctaagaaatc agtctttgcc cagcaggttg gactcaagga agtcgcaaat
     9121 tatctgggtg agattttcaa gcgtgttgga gctgaagtgg agattgatga gagctataca
     9181 gcgccctttg tcatggcaca tttcaagagt tcgcgtccag atgccaagac cttgattttc
     9241 tataaccact atgacactgt gccagcggat ggggatcagg tctggacaga ggatcctttt
     9301 acgctttcgg tccgcaatgg cttcatgtat gggcgtgggg ttgatgacga caagggtcat
     9361 atcacagctc gcttgagtgc tttgagaaaa tatatgcagc accatgatga tttacctgtc
     9421 aatatcagct ttatcatgga gggagcggag gaatcggctt caacagacct agataagtat
     9481 ttggaaaagc atgcagacaa actccgtggg gcggatttgt tggtctggga acaagggacc
     9541 aaaaatgcct tggaacagct ggaaatttct ggtggcaata aggggattgt gacctttgat
     9601 gccaaggtaa aaagcgctga tgtggatatc cactcgagtt atggtggcgt tgtggaatca
     9661 gctccttggt atctcctcca agccttacag tctcttcgtg ctgcggatgg ccgtatcttg
     9721 gttgaaggct tgtacgaaga agtacaagag cccaatgaac gagaaatagc cttgctagaa
     9781 acttatggtc aacgaaaccc agaggaagtt agtcggattt atggattgga gttgcctctc
     9841 ttacaggagg atcggatggc ctttctaaaa cgtttctttt tcgagccagc gcttaatatc
     9901 gaaggaatcc agtctggtta tcaaggtcag ggtgttaaga ctattttacc tgcagaagcc
     9961 agtgccaagc tagaggttcg tctggttccg ggcctagaac cgcatgatgt tctggaaaaa
    10021 attcggaaac agctagacaa aaatggcttt gataaggtag aattatacta taccttggga
    10081 gagatgagct atcgaagcga tatgagcgca ccagccattc tcaatgtgat cgagttggcc
    10141 aagaaattct atccacaggg cgtttcagtc ttgccgacga cagcggggac aggacctatg
    10201 catacggtct ttgatgccct agaggtacca atggttgcat tcggtctagg aaatgccaat
    10261 agccgagacc acggtggaga tgaaaatgtg cgaatcgctg attattacac ccatatcgaa
    10321 ttagtagagg agctgattag aagctatgag tagagatatt atcaagttag atcagatcga
    10381 tgtgactttt caccaaaaga agagaaccat tacagcggtt aaggatgtga ccattcacat
    10441 ccaagaaggg gatatctacg gaatcgttgg atattctgga gcaggaaaat caacccttgt
    10501 acgggtgatt aacctcttgc aaaaaccatc tgcagggaaa attaccattg acgacgatgt
    10561 gatttttgat ggtaaggtca ccttgacggc agagcagttg cgtcgtaaac gtcaagatat
    10621 cgggatgatt ttccagcatt ttaacctgat gagccaaaag acagcagagg agaatgtagc
    10681 ctttgccctt aaacactctg gactcagcaa ggaagaaaag aaggctaaag tagctaagtt
    10741 gttggacttg gttggtttgg cagatcgtgc tgaaaactac ccttcacaac tatctggagg
    10801 gcaaaaacag cgtgtggcaa ttgcgcgtgc cttggccaat gatccaaaaa tcttgatttc
    10861 agacgagtca acttctgccc ttgaccctaa gacaaccaag cagattttgg ccttgttgca
    10921 agatttgaac caaaaattag gcttgactgt tgtcttgatt acgcatgaaa tgcagattgt
    10981 caaagacatt gccaaccgtg tggcggttat gcaggatggg catttgattg aagagggtag
    11041 tgtgcttgaa atcttctcaa accctaaaca acctttgact caagacttta tctcaacagc
    11101 tacaggtatt gacgaagcca tggtcaaaat cgagaagcaa gaaatcgtgg aacacttgtc
    11161 tgaaaacagt ctcttggtgc aactcaagta cgctggagct tcaacagacg agccactttt
    11221 gaatgaattg tacaagcatt accaagtaat ggctaatatt ctctatggga atatcgaaat
    11281 tctcgatggt actcctgttg gagaattggt ggtggtcttg tcaggtgaaa aagcagcgtt
    11341 ggcaggtgcc caagaagcta ttcgccaagc aggtgtacaa ctaaaagtat tgaagggagg
    11401 acagtaagat ggaatcattg attcaaacct atttaccaaa tgtctataaa atgggttggg
    11461 ctggtcaggc aggctgggga acggctatct acttaactct ttatatgaca gttctttcct
    11521 tcattatcgg aggcttcttg gggctagtgg caggtctctt tctcgtcttg acagcgccag
    11581 gtggtgtctt ggagaataaa gtcgtattct ggattttaga caaaattacc tcaatttttc
    11641 gtgcggttcc ctttatcatc ctcttggcaa tcttgtcacc actttctcac ttgattgtta
    11701 aaacaagtat cgggccaaat gcagcccttg tcccactttc ttttgcagtc tttgccttct
    11761 ttgcccgtca ggtgcaggtt gtcttggctg aattggatgg cggtgtcatt gaggcggctc
    11821 aagcgagcgg agcgactttc tgggacatcg tgggtgttta cctatcagaa ggtcttccag
    11881 atttgatccg tgtgacgact gtgaccttga tttcccttgt tggggaaaca gctatggccg
    11941 gtgcggttgg agctggtggt atcggtaacg tagccatcgc ttatggattt aaccgctaca
    12001 atcacgatgt gaccatcttg gcaaccatcg ttatcatttt gattatcttt gcaatccaat
    12061 ccttaggaga tttcttgact aagaaattga gccataaata aaaaagagcc gtgtggctct
    12121 ttttaactga tcagattttc tgggcaaatt ttttactcaa ggcttgtcca atcaaggcac
    12181 ccactagggc tccgatgaca atacttgcga taaatagaag gacagttcca gggtttggag
    12241 cgaccatgat acggtcgata tattcttggg attttcctct tgccagaaga gtagccatat
    12301 aggctttggg cgcaatccac ataagcaaga ttggtcctgt tgtactaaag gcgaaaataa
    12361 tgaaagaaag gaagttcttt gttttgtcct tgtattttcc taaatgagct acttcatctg
    12421 ctaggaggcc acagataatt ccaggaagga aggaaccagc accgtgttta gttcccaaga
    12481 aaaagaaggc aatgacaagg ccgatagtgg taatggctcc aaagcgcgga acttttgcga
    12541 ctaggatcat atagacgcta ccgccgacaa gggcagtaaa ggcaggcgca taaaacatgt
    12601 ttccagtttg gtcaaagaga ttgcccaaaa ggacaccaat ccccatgcag aggaagtaaa
    12661 gaactgcaaa aagcagtgta gttaagatag atttcttcat gaattgtctc ctgataattt
    12721 tttcacaatt ctcattttac catggtttgg tgggattgta aatggatact agaagttttt
    12781 gaaaacgctt gaaatatgat ataatggttt catagttatt tttaggggaa tatcatgggt
    12841 agatttttag actttgtctt taatcgtttc tttttaggga tgattgcgac agccttcttt
    12901 tggctattaa ctttagcagg agggattatc cttggtctag cgccggctag tgccaccttg
    12961 atgagcttat atgcagaaca tggttatagc tttcgggaat acagtttgaa ggaggcttgg
    13021 tctctttaca agcaaaattt tgtctcaagc aacctgattt tctatagctt tttaggtgtg
    13081 ggtctagttt tgacctatgg tttgtatctc ttggtgcaat tgcctcatca gaccattgtt
    13141 catttgattg cgaccctttt gaatgtccta gtagttgccc tgatcttttt ggcttataca
    13201 gtatctttaa aattacaagt ttattttacc ttgtcctatc gaaatagtct caaattatcc
    13261 ttgattggca tctttatgag tctagcagct gtggctaagg ttctccttgg gactgtgcta
    13321 cttgtagcaa ttggttatta tatgcctgcc ctgctatttt ttgtaggaat tgggatgtgg
    13381 catttcttta tcagtgatat gttggaacct gtctatgaaa tcatccatga aaaattggcg
    13441 acaaaataga atgaagcagt tttggctaca tacgcttcta agaacctata gttcagtgat
    13501 gatcattatc attgcgagtt ttgcaatctt actctcttac gctgactggg attcacgtga
    13561 aaaggaagcc cagagagtag cccagcgtgt aactgctaga acagtgagtg aaattgaata
    13621 ttaccataga gagtcaaccc agatagctca ggctttagtt gaaaatcaag ctcgtattga
    13681 gggaatctat aaatacttta gccttagcat gccagactat ttttactggc aattagagcg
    13741 gaaagcttcg ccttatatat cagtctctct gtatgaaaat gttgatgacc tctatgttcg
    13801 aaatgatttt gtaactggag tggccattgc ttttcaagat tacaaggaag tctatgtttc
    13861 tactaaagac aaacgtagtg gagaaaaaat cagggctgag gatttcaaac cagcaggaaa
    13921 tagttttgcc attccggtgt cagatccagt gtcagatcaa gatttaggag tgatttacat
    13981 ctccttggat cctgctgttt tataccatgc cattgataat actagaggtc atactccgat
    14041 ggcagtaaca gtgacctcac cttttgatac ggagattttt catattggtg agacagttga
    14101 tagggagagt gaaaattggc tagttggctt aacttctcat ggatatcagg ttcaggtggc
    14161 agttcctaaa aactttgttt tacaaggaac agtgactagc tctgctttga ttgtgggttt
    14221 gagccttctc tttattgtca ttctttatct gactttgagg cagacttttg ctaattacca
    14281 aaagcaggta gtggatttag tagaatccat tcaagtcatt gctcaaggcg aagaggggcg
    14341 tcggattgac atttccgaaa aagatcagga attactccta atcgcggaga cgaccaatga
    14401 tatgttggat cgattggaaa agaatatcca tgatatttac cagttagagc ttagtcaaaa
    14461 agatgccaat atgcgagcct tgcaggcgca aatcaatcct cattttatgt ataatacgct
    14521 ggagttcttg cgcatgtatg cagttatgca gagtcaagat gagttggcag atatcattta
    14581 tgaattcagt agtctcttgc gtaacaatat ttccgacgaa agagagaccc tcctcaaaca
    14641 ggaattagaa ttttgccgta aatacagcta tctctgcatg gttcgctatc ccaagtccat
    14701 tgcctatggt ttcaagatag atccagagtt agagaatatg aagattccca agtttacctt
    14761 gcaaccgctg gtagaaaact atttcgcgca tggtgttgac cacaggagga cagataatgt
    14821 gattagcatc aaggctctta aacaggatgg gtttgtggaa attttggtgg tcgataatgg
    14881 tagaggaatg tcggctgaaa agttggcaaa tatccgagaa aaattaagtc agagatattt
    14941 tgaacaccaa gccagctaca gtgatcaaag gcagtctatc gggattgtca atgtacacga
    15001 gcgttttgtg ctctattttg gagaccgcta tgccattacg atagagtctg cagagcaagc
    15061 cggtgttcag tatcgtatta caattcaaga tgagtagaaa gggagaaaat gtataaagta
    15121 ttattagtag atgatgagta catggtgaca gaaggtctga agcgtttgat tccctttgat
    15181 aagtgggata tggaggtcgt cgcaacagcc agtcatgccg atgaagctct agaatatgtt
    15241 caggaaaatc ctgtcgatgt catcatttcc gatgtcaata tgccagacaa aacagggctt
    15301 gatatgattc gggagatgaa agagatctta ccagatgctg cctatatcct gctctcaggt
    15361 tatcaggagt ttgattatgt aaaaagagca atgaacctta gtgtggtgga ctatttggtc
    15421 aagcctgttg ataaggtaga gctgggaaat ctgctggaga agattgcagg tcagctcggc
    15481 gagagaggga agaaaagtca gactcttagt caagaattag acgaggctgg atttgttagt
    15541 tatttagggg ataaggagaa ttggtggata ggtctatcca aggaaaaaca aggttccttc
    15601 accattccct actatgtctt gggccaagac tggcagattt tcatttctga ccaaccccta
    15661 gatggtttag tcgttacacc ttttgaagct ccttatcaag aacactttga acgctggaag
    15721 ctgaatgctg agaaaaccct cttttacggt tctgtaaatt tgcagcagtc tgagagtctc
    15781 tttgcctatt acgaaccgat ttatagggtt atcattcagg gaaatctcaa tcaaatcgta
    15841 gaagagttaa atctcttgga gaaggtagtt cttgaaaata cgccgcgtgt tccgattact
    15901 aaacagcttt ttattcagtt tgtcatggat gttttccatt tatttgaaca tctcaaagct
    15961 gatgacctga cagacatcgt caaaaccatt catgctattc aatccttcga tgaattggtt
    16021 ccttatatca aggaaactct gaccagcttt ttcggtcaat accgtatgaa tgaaaatgtg
    16081 gtcagtgtgc tggaagtcat tggtcgtgat taccaaaaag agctttccct caaggatatc
    16141 agtaaggctc tctttatcaa tcctgtctat ctagggcagt tgattaagcg tgaaaccgat
    16201 tcgacttttg cagagttact aaacaaacaa cgtattaagg ctgcccaaca acttttgctt
    16261 tcaactagtg acagcatcga agatatttgt tatgctgttg gttacagtaa ccttggatat
    16321 ttctataaag ttttccgaaa attgtgcgga aaatcgccaa aagcctaccg aaaacaggta
    16381 gaaactatac tataaaattt gtattccttt acaaaaggtg ctataatatt atcaggaggt
    16441 tctatcatga aaaagaaacc aatttatcta tgggtcttgc taatcttgtc tgcccttatt
    16501 tcagttccgt ctctgtttgg aattgtgagt cccttgccta gtaaagaagc ccttcgtgct
    16561 gctcagaaac aagttgcagg ggtcaatgct cagcaattag aagaccagct taattatacc
    16621 tatagagtag cagaagcatc tcattctatt tttaatgttg ctttgattgt gctatctact
    16681 attttagtgg tcgtagcgat tgtcttcctt gttcgtaaaa atttgcaata tgcaaactat
    16741 acttacgttg gctatgtttt gttagctatt attggttcga tttatggcta tgttggttta
    16801 caagacgctg tgcagttggt tcaagatgag agcatgcgtt tgacagtgag tattggttca
    16861 aaagctgtta gcattttcta tatcgtcatc aatgttctct tcctagccct tgtcttctat
    16921 aagatgtggc gacaacagaa agccttggca gaagaagagg aaacagaaga gcttgcctaa
    16981 gtattgacaa aaaagaacta tcaagataca atcttggtag ttgttttttg attatagata
    17041 aaatcatgga ggtacatatg aagacaattt ctttagttta tattagtttg agtggcaaca
    17101 ctgaaagttt tgtgacgcgc ttgaaagact atctcttgtc ccagtacaaa gggattgagg
    17161 ttcaaaagat tcatatcaag gatttagtca aggaaggcaa aaatttctat gaaatggacc
    17221 atccctatgt cgcttttttg ccgacctatc tcgaaggtgg gaatggcgtg gataacggag
    17281 atgttgagat tttgacgaca ccggtgggag attttattgc ctatggtaac aatgctagta
    17341 agtgttttgg tgtggttggg tcaggaaatc gtaactttaa taaccaatac tgcctaacag
    17401 ccaagcaata cagtcaacgc tttggcttcc ctgtattggc tgactttgaa atgcgaggca
    17461 tgctggaaga tatcaaacat gtcgcagcta ttatcgcaga tttgtatgag ttggaaaagg
    17521 agaattaata ctcaatgaaa atcaaagagc aaactaggaa gctagtcgca ggttgctcaa
    17581 agcacagctt tgaggttgca gatagagctg acgtggtttg aagagattct cgaatagtat
    17641 aaagttctta taagaggtgg ttgaagttgt tcaactgttt tttgagtata aacagtcttt
    17701 aagaacgtaa aaaagcataa gttcaggtct tcaaaacctt gttcttatgc tttttatcat
    17761 agaaatattt gctgaatttt ctcttgaaat gaaaccttat cttcatctcg cgcttgtccc
    17821 ttaccctgct tttccgattg cgagtgcgta gataaagaag atggcgaagc caaagaggaa
    17881 aatcaatccc gcaatggcaa ggtgtttgag ccaagaagga agatttttgt tttcacgcgt
    17941 taccaaggcg taattcaaaa gagcaaagaa aggtgttgtc aggaaagatc caatcatggc
    18001 aaagcggagc atggttgaaa cctgaccagc gaagaacttg ataatgacga taccgatgat
    18061 agcagtgatg gtcatccaga tgttcaaaga tttacgattg tcctcttttt gactgattag
    18121 cagtcggaga gattcctgat taacgcgaga atagccatcg ataacagtta taactgttcc
    18181 aaagatacag aggaaggcaa taaaggtaat caagtaacgg gaccattcgc caagaacaga
    18241 ggcatacatg cccacgaatt gagagatgta tttggctgaa gcagcttcaa ccgcctgccc
    18301 tgtaggatac tgaatcagtg ctcccagtgc cacaaagaag acggctagga tagctgttcc
    18361 aatataacca gtgttaaagt caaacagagc gtcttctgtg ttaaagttga cggtctttct
    18421 cttttcagct gaccaaagtg aattgatggc tgaaatttca ataggagccg gcatccatcc
    18481 taggagggag acgatgaagg gcagagctgc catttgccaa ggtgtcttct cgacaaaatc
    18541 agaactgtat tctggatgct tgaccgccgc aatgataact gccagaacag tcgcaatggt
    18601 taaggcagac ataatccatt tgaccatgcc gtctaaaagt ttgtagcctc caaagagtag
    18661 catagcccaa atgattgcaa cgagaatgag ggaccactga gtaatgctaa gtccaatcat
    18721 tgggaaggca ctggcgatga tagctgagca cagaatggca acaccagccg tgttgaccat
    18781 agccgaaaag acattgagga taaagaaaat ccagagatag agttttcctt tttcggcata
    18841 accttcaacc aaagtctttc cagtatcagc tgtgtattca gcaccaaaac ggaaaaatgg
    18901 atatttaaag acattggcta agatgaccaa gagaagtaga gaccaaccgt aagaaccgcc
    18961 agcttgagtt gaggatacaa tgtgggaacc tccaacagcg gcagttgcca ttaggattcc
    19021 aggtcccatt gcttttagct tgcttgccca ggttgattga tttaaggaaa tagcttgcga
    19081 catgataaat actccttttt gaattttcag aataatctgt atatgtaatc tatattctac
    19141 aaaaaatttt ggaaaaatgc aagagtattt tgaaaaaaga tagaaaattt acaaagaaga
    19201 tctgaaataa ctattatgat atagcggatt gaaactagaa tagtacacct ttgcttctaa
    19261 aacattgtta gaaatctatt tgactgtcct gatcgattta tcctgttctt atttcatttc
    19321 actatagtaa ggtgcagcag gataaagaaa aaccgagaag tttctttctc ggatttgctt
    19381 attatgaggt tcctattttc tatgctccaa gagttgattg atgtgatcta cttttttagc
    19441 ctctctttta tagagaatag cccagatgat gagatagaca atcgcaaact cgataatgag
    19501 ctggaggtag aaaatccagt ggaaagggaa ccaaccagca agagttgcta gtgggacaaa
    19561 gccagccagc ataaggaaga aatgagtcag agtggcacga agcatgctcc agtcacggct
    19621 aaataagcgg ttgccaaagt tgaagagcat accgatggtt gcccagataa gtgtgcagta
    19681 gagcaagacc agggcaccgt gaacctgatg ctgatccatc acttggccga tgagagagta
    19741 gggatttagt ggtgcgtagg tatttggtgc ataaatgagt gaaaagagga tagagaggat
    19801 gaggccgata agaacaccgg tagctgcatc gtgaaatact tgttttttca tagttctaat
    19861 ttctccttga tggtttttag ataacggcgt gaagagtagg tgaagctttc gtttttcaag
    19921 aaaatttcta ccagaccgtt tggcgtgagc ttgaggtgag agatggaatc gatattgatg
    19981 atttctgatt gggaaatttg gacaaaattg gttggcaaga gtttaagaac ctgatagagt
    20041 cgcaaatcaa tgctgtaggt ctgactcgcg gtttctgcta gaaccttccg attctcgata
    20101 tagaagcgct gaatcttgcc aatctcaact agatagacct gatcatcgat ttttcctttg
    20161 attttttctc tttggtccag attttctgcg aactcgatga ctttctggac tttttcggtt
    20221 tcttgaggtg cttggacaat cagcttttcc tcctcgtaag tctcactaat ctgtagttct
    20281 actttcatag ttttctctcc ttttcagtta tacaaggttg tgatcacttc ctgtatatcc
    20341 ttattaaaca ctattttata gcccatggca aggaaccttg tctatgtgga gggattgggc
    20401 tcctatgtgg tggagctttt ctgttctttc tgaaatatgg tataatagca ctaatcaatt
    20461 tctaggaaaa tagatacaga aaggggctga aagatgtctc atattattga attgccagag
    20521 atgctggcaa accaaatcgc ggctggagag gtcattgaac gtcctgccag tgtggtcaaa
    20581 gagttggtag aaaatgccat tgacgcgggc tctagtcaga ttatcattga gattgaggaa
    20641 gctggtctca agaaggttca aatcacggat aacggtcatg gaattgccca cgatgaggtg
    20701 gagttggccc tgcgtcgcca tgcgaccagt aagataaaaa atcaagcaga tctctttcgg
    20761 attcggacgc ttggttttcg tggtgaagcc ttgccttcta ttgcgtctgt tagtgtcttg
    20821 actctgttaa cggcggtgga tggtgctagt catggaacca agttagtcgc gcgtgggggt
    20881 gaagttgagg aagtcatccc agcgactagt cctgtgggaa ccaaggtttg tgtggaggat
    20941 ctctttttca acacgcctgc ccgtctcaag tatatgaaga gccagcaagc ggagttgtct
    21001 catatcattg atattgtcaa ccgtctgggc ttggcccatc ctgagatttc ttttagcttg
    21061 attagtgatg gcaaggaaat gacgcggaca gcagggactg gtcaattgcg ccaagcaatc
    21121 gcagggattt acggtttggt cagtgccaag aagatgattg aaattgagaa ctctgaccta
    21181 gatttcgaaa tttcaggttt tgtgtccttg cctgagttga ctcgggctaa ccgcaattat
    21241 atcagcctct tcatcaatgg ccgttatatt aagaacttcc tgctcaatcg tgctattttg
    21301 gatggttttg gaagcaagct tatggttgga cgttttccac tggctgtcat tcacatccat
    21361 atcgaccctt atctagcgga tgtcaatgtg catccaacta agcaagaggt gcggatttcc
    21421 aaggaaaaag aactgatgac tctggtttca gaagctattg caaatagtct caaggaacaa
    21481 accttgattc cagatgcctt ggaaaatctt gccaaatcga ccgtgcgcaa tcgtgagaag
    21541 gtggagcaaa ctattctccc actcaaagaa aatacgctct actatgagaa aactgagccg
    21601 tcaagaccta gtcaaactga agtagctgat tatcaggtag aattgactga tgaagggcag
    21661 gatttgaccc tgtttgccaa ggaaaccttg gaccgattga ccaagccagc aaaactgcat
    21721 tttgcagaga gaaagcctgc taactacgac cagctagacc atccagagtt agatcttgct
    21781 agcatcgata aggcttatga caaactggag cgagaagaag catccagctt cccagagttg
    21841 gagtttttcg gacaaatgca cgggacttat ctctttgccc aagggcgaga tggactttac
    21901 atcatagatc agcacgctgc tcaggaacgg gtcaagtacg aggagtaccg tgaaagcatt
    21961 ggcaatgttg accagagcca gcagcaactc ctagtgccct atatctttga atttcctgcg
    22021 gatgatgccc tgcgtctcaa ggaaagaatg cctctcttag aggaagtggg cgtctttcta
    22081 gcagagtacg gagaaaatca atttattcta cgtgaacatc ctatttggat ggcagaagaa
    22141 gagattgaat caggcatcta tgagatgtgc gacatgctcc ttttgaccaa ggaagtttct
    22201 atcaagaaat accgagcaga gctggctatc atgatgtctt gcaagcgatc tatcaaggcc
    22261 aatcatcgta ttgatgatca ttcagctaga caactcctct atcagctttc tcaatgtgac
    22321 aatccctata actgtcctca cggacgtcct gttttggtgc attttaccaa gtcggatatg
    22381 gaaaagatgt tccgacgtat tcaggaaaat cacaccagtc tccgtgagtt ggggaaatat
    22441 taaaagtata aaaaagtctg ggaaaaattt tcaaaatcag aaaaacgcat aaaatcaggt
    22501 gttcaaaaac cttgatttta tgcgttttat catagaaata tttacttcat tttttcctaa
    22561 ttcttttcga aactcttttt aaacgacgtc agttttatca gtaatctcaa aacagtgttt
    22621 tgagctaatt ttgccagttt tgtctgtaac atcgaagttg tgtttgacca ctctgcgact
    22681 ggtttcctag tttgctctat gattttcaca gagtattaaa ttgcgatttt gccaagtttc
    22741 tttattcgtc taaaagtaga gtctgttcta tgcgtctaat gtacgaatta ggttgaccat
    22801 ttcaatagct ccttgtgcac actcagaacc cttatttcct gctttagtac cagctcgttc
    22861 tatggcttgt tcaattgtat ctgtcgttag cacaccaaac ataacaggaa tttcgctatt
    22921 taaactgatt tgggcgattc ccttagatac ctcgctacat acataatcat aatgacttgt
    22981 attccctcta atgacagctc ccaagcagat aattgcatca tattttttac tttttgccat
    23041 ttttgatgca atcagtgata tttcaaaagc tcctggaacc caggctacct ctatatcttt
    23101 ctcgtttaca ttctctcttt tgagattatc tagtgctcca gataataatt ttgaagttat
    23161 aaattcatta aatctcgcta caacaatacc tattttaata ttgtttgcta ctaaattacc
    23221 ttcataagtg ttcatttatt tttcctccat atttaaaatg tgacccattc gatttttctt
    23281 tgtttctaaa taaaaactat cgtaaggatt ggcttctatt tcgattgata ttctactgga
    23341 aatagtaatt ccatattttt ctaactgttc aaccttgtca ggattatttg tcagtaaatg
    23401 aagtgactga agtcccagat ctttaagcat ttgtgctcca atatgatatt ctcttaaatc
    23461 accttcaaag cctaatgcaa gattggcatc aagcgtatcc atgccttgat cttgtaaatg
    23521 ataggctttt aatttattga taagtccaat tcctcgtccc tcctgtcgca agtaaagtaa
    23581 gacacccgaa ccattctcaa caatcatttt catagcttta tcgaattgct gtccacaatc
    23641 gcaacgtaaa gagcctaaaa catcccctgt taaacattcg gagtggatcc gacataatac
    23701 attggcttca tcctctatgt ttcccataat aagagcaaga tgatgttccc catttagttt
    23761 atctatatag ctaattgctt tgaaattacc gtatctagta ggcatattga cagttgaaac
    23821 tcgttctacc agctgatcat atacttttct atattcttgt aattctttga tggtaattag
    23881 tggaatgttg tgtttcttcg agaactgaat taaatcatct gttctcatca ttttgccatc
    23941 atgattcatt atttcacaac ataggccaca ctcttttagt ccagctaatt ttaataaatc
    24001 aacagttgct tctgtgtgtc catttctttc tagaacacca cctttttttg caattaaagg
    24061 aaacatgtgt cctggcctgc gaaaatcaga gggtgttata tcttcagcta cacacatacg
    24121 tgcggtcagt cctctttcct cggcagaaat acctgtggtc gtttctttat aatcaattga
    24181 aactgtaaaa gcagtcttat gattatctgt attgttttca accataggtg aaagcattaa
    24241 ttgattagct aaactttcgc tcataggcat acaaattaat cctttggcat aagtagccat
    24301 aaaattaaca ttttctgttg tagctgcttg tgcagaacaa attaagtctc cttcattttc
    24361 tctatccttg tcgtctataa caagaacaag tcgtcccttc tgcaatgctt ctaatgtttc
    24421 ttgtattttt cgatattcca ttgactgatt atcctttctg ctaaaatcca ttttgatata
    24481 atagttcctt agatatttct gattttggag agttatccat cagtttttgc acatatttac
    24541 ctaagatatc attttcaaga tttactgtac tcccgacttg tttactttta agaatggttt
    24601 gtttcaaggt atgagggata acagatactg aaaagtttac tttggagact ttagcgacag
    24661 tcagactaat gccgtcaatt gtaatagatc ctttttcaac tattaaatct aaaatttctt
    24721 tttgtgtgtt gatttgatac catacagcat tatcatcttt ttttattgac gagatttttc
    24781 ctgtaccatc aatgtgtcct gtaacgacgt gacccccaag tcgaccgttg atagataagg
    24841 ctctttctag attcacctca cttccatgtt ttaatagagt aagagctgtt cgactccatg
    24901 tttcattcat tacatcaact gtaaaggatt gatgattgaa atgagtaact gtaagacaga
    24961 taccatttac tgctatacta tcgcctaaat ggatatccgt taatattttt gaggctttaa
    25021 ttgatagttt acaattacga gagtctttct gtattctttc aacttttccg atttcttcaa
    25081 ttattcctgt gaacatggat aaatcacttc actttctatg agatagtcat ttcctatttg
    25141 agaaaaagca taaggtttca atctaatagc gtcatttggc aaagaaatgc cttcacctcc
    25201 gacaggaaac ttggcactgc ctccaaaaat ttttggtgca atatatattt tcagctcatc
    25261 aacaatttgt tgttccaaag cactccaatt cattagactg cccccttcta gaactaggct
    25321 atcaatctgc atgtttccta gatgttgcat taaactcgat aagtctatat gattgccttt
    25381 tttctttatg gaaagtattt cacagccatg attttgatat agcttcattt tatttttgtc
    25441 ttcagaggaa gtggcaatgt aagttttaat atcatttgct gtttttacga ttttagaggt
    25501 aagaggagtt cgtaaatgtg tatcgcatat gatacggata ggatttttcc cttcctccaa
    25561 tctacatgtc agcaaaggat cgtcttgaat aacagtattg actcccacca taattgcact
    25621 aacatggtgt cgtaactgat gcacatgctt tcttgcttct tcttcagtaa tccatttgga
    25681 ttgatttgtt ttagtggcta tttttccatc cattgacatt gcatatttca taaaaacata
    25741 gggtacatgc tgggtaatat actttctaaa actttttatt aagttaagac actcattttc
    25801 taaaattcca acagtaactt gaagattatt ttcctcaagt atctttactc cttttccaga
    25861 tacaatagga ttacagtcta ggcttccaat gactactctt gtaataccac tatcgattat
    25921 agcatctata cagggaggtg ttttcccgaa gtgacaacag ggttcaagtg ttacataaag
    25981 cgtcgctccg acaggggatt ctctacagtt tttaagagca tttctctcag catgtgggcc
    26041 accaaaaaac tcatgataac cttgtccgat gatgtgatta tcttttacaa taactgcgcc
    26101 gaccatagga ttgggattga cgtaaccagc ccctttttgt gccagtttta ttgctaattt
    26161 catatatttt gaatcgctca tctcactacc tccaaaaaaa atataccttg aataggggac
    26221 tactcaaggc atacaaaaga aaacttatgc gattaacaaa aatactctga aatgacaagt
    26281 aatcatttca gagcacgcaa aaagcacaaa tatactttta tcttctttca tccagactat
    26341 actgtcggct ttggaatttc accaaatcat gcctttcggc tcgtgggcta taccaccggt
    26401 agggaattac accctgccct gaagatagtt attcaattac agatgattat agtacttaat
    26461 tttgaatatg tcaacagata aataccgatt gtttttgata tactgtattt gtgataatcg
    26521 attctcgctc ctcggataaa gaaaatatga tatactagat aaacgaaata agagagaagg
    26581 aatactatgt acgcatattt aaaaggaatc attaccaaaa ttactgccaa atacattgtt
    26641 cttgaaacca atggtattgg ttatatcctg catgtggcca atccttatgc ctattcaggt
    26701 caggttaatc aggaggatca gatttatgtg catcaggttg tgcgtgagga cgcccatttg
    26761 ctttatggat ttcgctcaga ggatgagaaa aagctctttc ttagtctgat ttcggtctct
    26821 gggattggtc ctgtatcagc tcttgctatt atcgctgctg atgacaatgc tggcttggtt
    26881 caagccattg aaaccaagaa catcacctac ttgaccaagt tccctaaaat tggcaagaaa
    26941 acagcccagc agatggtgct ggacttggaa ggcaaggtag tagttgcagg agatgacctt
    27001 cctgccaagg tcgcagtgca agcaagtgct gaaaaccaag aattggaaga agctatggaa
    27061 gccatgttgg ctctgggcta caaggcaaca gagctcaaga aaatcaagaa attctttgaa
    27121 ggaacgacag atacagctga gaactatatc aagtcggccc ttaaaatgtt ggtcaaatag
    27181 gagcagagaa tgacaaaacg ttgttcgtgg gtcaagatga ccaacccgct ctacatcgcc
    27241 tatcatgatg aggagtgggg tcagcccctc catgatgacc aagcattgtt tgagttgttg
    27301 tgtatggaaa cctatcaggc aggcctgtct tgggaaacgg tactcaacaa acgccaagct
    27361 ttccgagaag cctttcatag ctatcaaatt cactcagtcg cagagatgac tgagactgaa
    27421 ttggaagcca tgctggagaa tccagctatc attcgaaata gagccaagct ttttgctaca
    27481 cgcgctaacg cccaagcctt tctacagtta caggcagagt acggctcttt tgatgcctat
    27541 ctttggtctt ttgttgaggg gaaaactgtc gttaacgatg ttcctgatta tcgccaagcc
    27601 ccagctaaaa cacccttatc tgagaaatta gccaaagatc tcaaaaaacg aggcttcaag
    27661 ttcacaggcc cagtcgccgt attgtctttt ctacaggctg cagggctagt tgatgaccac
    27721 gagaatgatt gtgagtggaa aggtcttaaa tgatgtctaa caaaaataag gaaattctga
    27781 tttttgcgat tctctataca gtcctctttg tgtttgatgg cgttaaattg ctggcttctt
    27841 taatgccatc tgccattgca aattatcttg tttatgtagt tttagctcta tatggctcct
    27901 tcttgttcaa ggatagattg atccaacaat ggaaggagat tagaaagact aaaagaaaat
    27961 tcttctttgg cgtcttaaca ggatggctct ttctcattct gatgactgtt gtctttgaat
    28021 ttgtatcaga gatgttgaag cagtttgtgg gactagatgg acaaggtcta aatcagtcta
    28081 atattcaaag tacctttcaa gaacaaccac tactgatagc tgtttttgct tgtgtcattg
    28141 gacctctggt agaagaatta tttttccgtc aggtcttatt gcattacttg caggaacggt
    28201 tgccaggttt actaagcatt attctggtag gacttgtttt tgctttgact catatgcaca
    28261 gtttggctct atcagagtgg attggtgcag ttggttactt aggtggaggc cttgcctttt
    28321 ctattattta tgtgaaagaa aaagagaata tctactatcc cctacttgtt cacatgttaa
    28381 gcaacagcct ctccttaatc attttagcta tcagtatagt aaaatgaaat gagaacagga
    28441 caaatcgatt tctaacaatg ttttagaagt agaggtgtac tattctagtt tcaatatact
    28501 gtaatatgtg atgaaaatgc cagtaatgat accgagaaaa aagctgagaa acttttccca
    28561 gctttatttg ttatagtcaa agagaatgac ttgttcctgt gcatctacat gagcatggac
    28621 cccaaagggt acaattgctc ttggagttgc gtggccgaca ttcagattat agacaatcgg
    28681 gatattgctg tcaatgatat ccaatagtgc ctctttatag tcgtcatgga aagtttcatc
    28741 cataggtttt ccgaccaaga gtccattgat gaccgcgaat atgccagtgt cctttaaagt
    28801 tagcaacatc tttttgaagt cttctgactt aggcttttct tcgcttgttt cgagcaagag
    28861 gattttccct tcccagtctg acaagtcagg gaaaagtttg tatttttggc agagttccgt
    28921 gctatctgcg tatcgagagt tgtcaaagat atcgtagagg gattcgaggc aaccaccgag
    28981 gattttcccc tcgaactggg cccttccttg caacaagtca aaacctgtat ttgtatgact
    29041 gacacgaggt gttcccaggg ccgtgggact aaaatcagtt cgttcctcat accaaacgtc
    29101 actagggcgg atttctgaaa ttcttcccgt ctcaatcaat tctttaaagt agtgaaggct
    29161 ataggctagc atttctttgt ctaattcaca aatgtctgct aaaaaggatt gaccataaaa
    29221 agtcttgatt cctaatttat gcaacatgag gtggttcatg gttgtatccg agaagccaag
    29281 aaaaattttt tgtttgataa ccttttggag ttggtcattt tcaaaaagat aaggtagcaa
    29341 gcgataggta tcgtctccac cgatggcaca taggatcatg tcgatgctat catcagaaaa
    29401 ggcatgaatc aaatcctctg cacgagcttc aggatggtcc ttgataaagt ctaatccttt
    29461 taacgaatgg ggcaaaaaga tgggattgag tcccagatcc ttgagacgtt ggatacccaa
    29521 gtccacttcg tgtttgacaa aatcctctcc gataatgcca ctagataaac taacaatacc
    29581 aatagtagaa accatatctc atcctcctag aaatagattg agcctatttt atcacaaaat
    29641 ataagagaaa gctatgtggg atttcagaag aatactttta atactcttcg aaaatctctt
    29701 caaaccacgt cagttttatc tgcaacctca aagctgtgct ttgagcaacc tgcagctagc
    29761 ttcctagttt gctctttgat tttcattgaa tataactcaa aaaagtaagt gaaaaagcaa
    29821 ccgcacctgc agttgctttt atcgttttct tattcttgat agcgtgttgg tcctgcactt
    29881 gcactacgga tattcaagcg tttcttgaga tagaagcgaa gggctaggag ggctgctcca
    29941 ataatagcta gtggcaatgg atccagtact gggttaaggc tagctggtag gaagcttgtt
    30001 gcaaagaaga caagcaacca aaggaacata gaagctagga taactagtac agatttccag
    30061 aaaggtggac gttgactgcg atccatatct ggtccatagt attggtaaac aaagtagtac
    30121 atcaagtaga aggcaaatcc accaaccagt ccaactaata gaagagtaat caatccatag
    30181 ccgaaagctt ggtctgccgc aaagaaggtt gtgagggcgc tgacaagggc aaagaggcta
    30241 gtgatgaaaa gagctgagtc cataatcatc agttttgggt catcattttc ttttggatgc
    30301 tctttttcgt actgctcttt gacagtgaag ctatgagccc aatgagttgg tgcgccgtat
    30361 agggaacggg cagttgtacc tttagattgc tcctcaagga tttggggaat gacttcctca
    30421 aaaatagtct ggatttcagc gtctgttttc ccatctttga tgaattgttg ggtagcaatg
    30481 tggacaaact cttggttttt cttagttaat ttttgtagat caatttgaga cataggaact
    30541 cctcttagaa ccatttttta tggatgagat agagagtgag cgagacactc atagcaaagg
    30601 cgataaagac gattaaccag aaggcatttg gctctccgtt tagggggatt tcattatcct
    30661 taaagttcat cccgtaggca gaaaagacca tggttgggat ggacatgacg atggtcacaa
    30721 gggccaaggt tttcatgatg ttgttctggt tgttagaaat gatagaggca aaggtctctg
    30781 tcatagaatg caagacgttt ccataaatat ctgccatctc gatggcctgt tgggtttcaa
    30841 tcagggtgtc ttcaagcagg tcttcgtcct caaggtattt cttgatattg ctggttgaac
    30901 tggtcaattt cttaatcacg cgctcatttg ttttgaggga ggccttgaaa tagacgatag
    30961 ttttttccaa ttccatgagc tcaatcaatt cttcattacg agttgattga tgcagttgac
    31021 tttcgatttg ttcactcttg cggtcgattg aacgaagggc tgttaggtaa agctctgcat
    31081 tgcgataaag aatttgaaag ataaaacgtg aacgcatgaa ggtatagaaa ttacgcaatc
    31141 gacggttgat aaagacatca aggacaggta gtggttccaa acacgtagtg ataatggttt
    31201 cctcagtgat gataatacca agcgggatgg ttacgtagta ggtgcggtta tttctttcct
    31261 ccgtgaccgg cacgtctacg ataatcaggg tatactcgtc ttcaatggta atacgagaca
    31321 tttcttccgc atcgagcggt gctcgaaggt cggcaatatc aatatcgaag gtgttggcga
    31381 tttcgagtga ttcattttga gtcggattga cgagattgat ccaagtaccc ggttcaagcg
    31441 tatcgatctc tttaaattca gttgttgtag agagaaaaac ttgtttcata tcccttatcc
    31501 cttatccttt ctcatttttc agattttttc acactgtact attatactac aaaatcggcc
    31561 tttttcaaaa taaatctaac aaaaaacctg ttgctattgg atttttgctt tgtcttgaaa
    31621 tgttgatttt taaatttgct ccccctatag tgcttttagt gcagtttcgt agaatgatac
    31681 tgcttttttt gcgttctctt ttgagaggtg gctgtatata tccatggtca ttgataaagt
    31741 ggaatgacct aggcggtact ggagttcttt gtatggtatt ccagagttaa gcaagagact
    31801 agcgtgagtg tgacggaatc cgtgaaagcc aatgttagga acgtcagcac gcttaaaatg
    31861 tgtttgcaat cttcttttaa gtcttgaact gctaggatac tcatgaatga agtcagaaaa
    31921 tactacactt tcacgttgcc ctatcttcca agcctcttta gtttggcgta gtttgtactg
    31981 cttcagcatg ctaacagtcg cttgatctat atctatatcc cgataactag ccttagattt
    32041 tgggctatta atttctaaat cacgatttag tgttttggta acatgtacca cggcattatc
    32101 taaatctata tcagaccagg aaagagctaa agcttcattg atacggcaac cagtagccaa
    32161 taggaactta tagagcgttg tctcatagta gtaacgatat ctatcgctat ctaaattatc
    32221 gagataacca agaaactttt ttagttcttg attttcaaag tgttttactt ttttccgctt
    32281 tgctttctga gtgttacgag gtaaaacaac atcacgggca ggattggacg gtatagcctg
    32341 catagttacg ccatactgta agatacgttt gtttagagcg tgtatcttgt cataatgtag
    32401 aaaagcccct ttttcccctc tattagtctt gtcagcaagt tcgttgataa ttgactggat
    32461 aagtggagta gtgagtttat cgagcttata agccccaaaa ataggcaata tatgattatc
    32521 aattaacttt ctaacattgt cttgagtgtt tggcttaacc gtattcttat aactttctaa
    32581 ccagagattg gctaattctt gatagttttt tatggtgcta gccttttgtc tagtagatcc
    32641 tgctttttga aaagctattt tttcttgagt agctttctgc ttaacttcct tttgtgtccg
    32701 tcccgttacc ttagttttaa ctttctttcc tgtaacctgg tcaactccta gatatatatt
    32761 agcacgatag actttactac cgtctttttt tataacctca gttattttca tgatttaaac
    32821 ctttctaaaa tacatcagca ggcaagccat tatcaaaaag gttttatatc atgctaggtc
    32881 ttcacagatt gccctgtatt cgattttaat tagtcaggcg gtaaataata ccaaagtttg
    32941 aaacaaggcg gatatggggc ttatatgggc ttgaagctcg aacgaagctt gagaaatcac
    33001 gcgcagaacg tgaaaaaaca tgaatttttt gaagatttca aaagctagaa aaaccagtac
    33061 ttacaagtct ttcaatggtt ttaagttatt tgggttcatg ttgacaaaag ttgacatttt
    33121 cagagtataa cataccaaaa aactaggaaa acttatattt cagtttttca agatagtaac
    33181 aaatgaaaat ttttaaaaag gtaactcaaa tttgcaatca acaatttttg aactaacaat
    33241 aatttgaata tcttcgctta aaagctggtc aacatgtcct gaagaatttt ttgcaaaa
//
LOCUS       DAVKWZ010000020        32959 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000020 DAVKWZ010000000
VERSION     DAVKWZ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32959
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.020"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            1..1118
                     /locus_tag="VW854_000967"
                     /pseudo
     CDS             1..1118
                     /locus_tag="VW854_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            complement(1407..1877)
                     /locus_tag="VW854_000968"
     CDS             complement(1407..1877)
                     /locus_tag="VW854_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEV0262221.1"
                     /translation="MRMPLKHPLKNRKKEKNMSKEKVILAYSGGLDTSVAITWLKKDY
                     DVVSVCMDVGEGKDLDFIHDKALKVGAVESYVIDVKDEFATDYVLVAHQSHAYYEQKY
                     PLVSALSRPLISKKLVEIAHQIGATTIAHGCTGKGNDQVEYQIAVAKKANEAKK"
     gene            complement(1843..2649)
                     /locus_tag="VW854_000969"
     CDS             complement(1843..2649)
                     /locus_tag="VW854_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV0262222.1"
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVAL
                     NPDFAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKAD
                     LAISGVSKTDERSKVFDFSTPYYTAKNKLIVKKSDLATYQSVNDLAQKKVGAQKGSIQ
                     ETMAKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVAKGFVENNPDLAIADLNF
                     EKEQDDSYAVAMKKDSKELKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            complement(2855..3496)
                     /locus_tag="VW854_000970"
     CDS             complement(2855..3496)
                     /locus_tag="VW854_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0262223.1"
                     /translation="MIELKNITKTIGGKVILDNLSLRIDQGDLVAIVGKSGSGKSTLL
                     NLLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLML
                     ALKYVKLPKKDKLKKVEEILERVGLSATLHQRVSELSGGEQQRIAVARAILKPSQLIL
                     ADEPTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRVIELGEGK"
     gene            complement(3493..5601)
                     /locus_tag="VW854_000971"
     CDS             complement(3493..5601)
                     /locus_tag="VW854_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV0262224.1"
                     /translation="MKKISNFCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDL
                     YLKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLD
                     FTTDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEM
                     DKDRIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQP
                     MMEMKRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPML
                     WLSHFLLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLIFNYVMKIGTTILLTAL
                     LIGVGRSLEQENKELAYQQQWVSQGNYLTLETFKLNDNLWQEELAGSGKSTDYFYRFY
                     QDLVEKTQAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPNTG
                     IKKVILMPASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFP
                     YSDKRKENLSNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKL
                     SDGNYLKFSSIQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVTTFLLKRRD
                     IITKKFMGWKLVDRYRPLLVLLLLGYSFPLLVLIFFAHAFLPLLLFAGFTCLDILFVL
                     GLASRMEKRSLVELLKGGIL"
     gene            complement(5653..5940)
                     /locus_tag="VW854_000972"
     CDS             complement(5653..5940)
                     /locus_tag="VW854_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEV0262225.1"
                     /translation="MRKLVKIGVASLMVCGILATTNALAVWVEGGQWNYGVGWTGTFG
                     YSDYLHSTRYHTATVRHGGRTSKDYAKPDAWARASLTKIPPTGMEYFYGFE"
     gene            6371..6691
                     /locus_tag="VW854_000973"
     CDS             6371..6691
                     /locus_tag="VW854_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV0262226.1"
                     /translation="MQRLEVYKNYQRLYDLRIAILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVRIGICTDNAKLIQKGFSLLELTEETSMLSHLKKEVEIYYQ
                     AKER"
     gene            complement(6743..7330)
                     /locus_tag="VW854_000974"
     CDS             complement(6743..7330)
                     /locus_tag="VW854_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV0262227.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVGQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            7572..8243
                     /gene="sdaAB"
                     /locus_tag="VW854_000975"
     CDS             7572..8243
                     /gene="sdaAB"
                     /locus_tag="VW854_000975"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEV0262228.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            8252..9124
                     /gene="sdaAA"
                     /locus_tag="VW854_000976"
     CDS             8252..9124
                     /gene="sdaAA"
                     /locus_tag="VW854_000976"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEV0262229.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIKNEKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            9146..9778
                     /locus_tag="VW854_000977"
     CDS             9146..9778
                     /locus_tag="VW854_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV0262230.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAVQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKNTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLVPQQAIII
                     GDTKFDMLGARETGIQKLAVTWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            9804..9902
                     /locus_tag="VW854_000978"
                     /pseudo
     CDS             9804..9902
                     /locus_tag="VW854_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(10083..11933)
                     /locus_tag="VW854_000979"
     CDS             complement(10083..11933)
                     /locus_tag="VW854_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV0262231.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(11972..13132)
                     /locus_tag="VW854_000980"
     CDS             complement(11972..13132)
                     /locus_tag="VW854_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV0262232.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTETFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREYGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGLGTIVGLIRGFKWKKEYKRTIIYLDKISQINQNMLQ"
     gene            13190..14356
                     /locus_tag="VW854_000981"
     CDS             13190..14356
                     /locus_tag="VW854_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEV0262233.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     IGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TAMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPAVAALLLIDILMTVALFTVDRADKIL"
     gene            14496..14822
                     /locus_tag="VW854_000982"
     CDS             14496..14822
                     /locus_tag="VW854_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV0262234.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWALYTDTINGIIEGS
                     IRHDKN"
     gene            14809..15402
                     /locus_tag="VW854_000983"
     CDS             14809..15402
                     /locus_tag="VW854_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEV0262235.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGIEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVTRFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            15395..16306
                     /locus_tag="VW854_000984"
     CDS             15395..16306
                     /locus_tag="VW854_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEV0262236.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQYDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            16368..17432
                     /locus_tag="VW854_000985"
     CDS             16368..17432
                     /locus_tag="VW854_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262237.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADLDTPIEK
                     NTQLEEEVSQAEVELESQQEEKIEAPEDSEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPYQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKFETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(17504..18364)
                     /locus_tag="VW854_000986"
     CDS             complement(17504..18364)
                     /locus_tag="VW854_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HEV0262238.1"
                     /translation="MAKTFFIPNKQSILGEQEILTAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYEAIARLDYAKFLEVLPLSFYHQLDANQIEIQPILG
                     QDFKTLAQEK"
     gene            complement(18486..19472)
                     /locus_tag="VW854_000987"
     CDS             complement(18486..19472)
                     /locus_tag="VW854_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEV0262239.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(19744..21219)
                     /locus_tag="VW854_000988"
     CDS             complement(19744..21219)
                     /locus_tag="VW854_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEV0262240.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(21477..22400)
                     /locus_tag="VW854_000989"
     CDS             complement(21477..22400)
                     /locus_tag="VW854_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV0262241.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(22414..23343)
                     /locus_tag="VW854_000990"
     CDS             complement(22414..23343)
                     /locus_tag="VW854_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV0262242.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(23662..24012)
                     /locus_tag="VW854_000991"
     CDS             complement(23662..24012)
                     /locus_tag="VW854_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262243.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKALTDDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETADKDVLENSQEELQKAVLFLADEKGSEHTEAELI
                     DNLKEVIAKLKANA"
     gene            complement(24438..24593)
                     /locus_tag="VW854_000992"
                     /pseudo
     CDS             complement(24438..24593)
                     /locus_tag="VW854_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(24778..25389)
                     /gene="rpsD"
                     /locus_tag="VW854_000993"
     CDS             complement(24778..25389)
                     /gene="rpsD"
                     /locus_tag="VW854_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEV0262244.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(25584..26636)
                     /locus_tag="VW854_000994"
     CDS             complement(25584..26636)
                     /locus_tag="VW854_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEV0262245.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(26633..27331)
                     /locus_tag="VW854_000995"
     CDS             complement(26633..27331)
                     /locus_tag="VW854_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0262246.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(27498..29159)
                     /locus_tag="VW854_000996"
     CDS             complement(27498..29159)
                     /locus_tag="VW854_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PavB family fibronectin-binding SSURE repeat
                     adhesin"
                     /protein_id="HEV0262247.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQENPELTDALKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKSTVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVEKAVKDNVKDSIDVPAAYLEKAKGEGPFTAGVN
                     HVIPYELFAGDGMLTRLLLKASDKAPWSDNGDAKNPALSPLGENVKTKGQYFYQVALD
                     GNVAGKEKQALIDQFRANGTQTYSATVNVYGNKDGKPDLDNIVATKKVTIKINVKETS
                     DTANGSLSPSNSGSGVTPMNHNHATGTTDSMPADTMTSSTNTMAGENMAASANKMSDT
                     MMSEDKAMLPNTGETQTSMASIGFLGLALAGLLGGLGLKNKKEEN"
     gene            complement(29275..29685)
                     /locus_tag="VW854_000997"
     CDS             complement(29275..29685)
                     /locus_tag="VW854_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEV0262248.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKCIDQQLKVNRLLVDQLY
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSGTAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            complement(29752..30291)
                     /locus_tag="VW854_000998"
     CDS             complement(29752..30291)
                     /locus_tag="VW854_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV0262249.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQENKGHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQEFESKGQEVDVFVTNFVYEKEGQSCKKSMSYDSVLPVRQIFGWDQVGNFSKGQ
                     YIMMHSLIYRTDLLRASQF"
     gene            complement(30437..30532)
                     /locus_tag="VW854_000999"
                     /pseudo
     CDS             complement(30437..30532)
                     /locus_tag="VW854_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(30646..31311)
                     /locus_tag="VW854_001000"
     CDS             complement(30646..31311)
                     /locus_tag="VW854_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEV0262250.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            complement(31325..32704)
                     /locus_tag="VW854_001001"
     CDS             complement(31325..32704)
                     /locus_tag="VW854_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV0262251.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
BASE COUNT         9858 a         7176 c         6039 g         9886 t
ORIGIN      
        1 tactagaatt ctccttagaa agcgtcgttt caagtgctat cattgttcta aaattatggt
       61 tgctgaaact tctatcgtca agaagaatca tcaaatccct cgtatcatca accaaaagat
      121 tgctcaaaag ttaattgaaa agatttctat gactgatatt gcccatcagc tttccatctc
      181 aacttcaact gtcattcgaa agctcaatga ttttcacttt aaacatgatt tttcttgtct
      241 tcctgagatt atgtcctggg acgttgaaac agtccgagga gtgactgttt caatcgggag
      301 atggagatga gcttcattgc gcaagatttt aacaatctta atattatcac tgttcttgaa
      361 ggcagaacac aagctgtcat ccgaaatcac tttcttcgct acgatagagc cgttcgttgt
      421 caagtgaaaa tcattacgat ggatatgttt agtccttact atgacttggc taaacagctt
      481 cgctttcaaa tttctaggct caggctgaaa cagtctccca gactgtttca ctcccgaatg
      541 ctaaaatcgt tcttgatcgc tttcacattg tacaacatct tagccgtgct atgagtcgtg
      601 tgcatgtcca aatcatgaat cagtttcatc gaaaatccca tgaatacaag gctatcaagc
      661 gctactggaa actcattcaa caggatagct gtaaactgag tgataagcga ttttatcgtc
      721 ctatttttcg tatgcactta accaataaag agattttaaa caagcttttg agctattcgg
      781 aagacttgaa acaccactat caactctatc agctcttgct ttttcacttc cagaataagg
      841 aaccagagaa atttttcgga cttattgagg acaatcttaa gcaggttcat cctatttttc
      901 agactgtctt taaaaccttc ctcaaagata aagaaaagat tatcaacgcc cttcaactac
      961 actattctaa tgccaaactg gaagcgacca ataatctcat caaacttatc aagcgcaatg
     1021 cctttggttt tcgaaacttt gaaaacttca aaaaacggat ttttatcgct ttgaacatca
     1081 aaaaagaaag gacgaaattt gtcctttctc gagcttagct gacttcaacc cactacagtt
     1141 gacaaagagc cttatttgtt cgttagttct taataccagc cattgttaaa ccagaagttt
     1201 ttagctctgc caagccacat agtgtgttta ctactatgtg tccatgtcac catgaatcgc
     1261 cgaacgattt aacgagaaag tatgactttt acgtttatcc caactcaatt atgacatttt
     1321 tttcaaaagt caatatatct cactttttca acgacaagaa agaggctacc aatcgaccaa
     1381 cctcttattc tgaacccatc actccatcac tttttagctt cattcgcttt cttagcgact
     1441 gcaatctggt attcgacttg gtcattcccc ttaccggtac aaccatgagc aattgtagtc
     1501 gctcctatct gatgcgctat ttcaaccaat tttttagaaa tcagagggcg gctcaaggca
     1561 gataccaagg gatacttttg ttcataatag gcatgtgact gatgagccac tagcacataa
     1621 tctgtagcaa attcgtcctt aacatcaatg acataagatt ctactgcccc aaccttaaga
     1681 gccttatcat ggatgaaatc taagtctttc ccttcaccca catccataca aactgaaaca
     1741 acatcatagt ctttctttaa ccaggtaata gcaactgatg tgtccaaacc gccagaatag
     1801 gccaaaatta ctttttcttt actcatgttc ttttccttct ttctattttt caatggatgc
     1861 tttaaaggca tcctcaatga gtttgtctaa ttccccagac tccttcaact tttgaatggt
     1921 tttatcgact gcctctttca attccttgct atcttttttc atggctaccg cataggaatc
     1981 atcttgctct ttttcaaaat tgaggtctgc gattgctaaa tcaggattat tttccacaaa
     2041 tcccttggca acgggttctt caaaaataac ggcatccact tgccctgatt ttaaatctgt
     2101 gattaaattc ccatttttag gcagagatac gagggaagaa ttttgtagca aatctttcgc
     2161 catcgtctct tgaatcgaac ctttctgcgc tccgaccttt ttctgcgcca aatcgttgac
     2221 agactgataa gtggctaaat cagatttttt gacaatgagt ttattttttg cagtatagta
     2281 gggagttgaa aaatcaaaca ccttgctccg ttcatctgtc ttagaaacac ctgatatggc
     2341 aaggtcagct tttcctgatt gaacgctagc cagtacattg tcaaaactca taggagatag
     2401 ttccaattcg acacctagtt ctgttgcgat agccttggct aattcgatat ctgaacccac
     2461 aatctgattt ttcccatcaa ccactttttg atattcaaat ggagcaaaat ctggattgag
     2521 ggctacaacc aattttcctt tggacttgat ggcttcaatt ccagccgatt gattgttact
     2581 acaagcaaat agtaggggga gcatcactaa accaaacatc gtcatcaaca ccttcttcat
     2641 cttattcata acaaagcctc ctttatgttt attctttttt attgtataaa taatactcct
     2701 atactcatcc tttgtcaagc ctaaattgaa aaaattttca atttgaaaca aaaaaaccta
     2761 gaaatactac ataaaatgtc attcctaggc gtatttatgc tatttttctc tagaaaacat
     2821 gagtattcag ccagtcaaaa ggagctgaat gaactcattt tccctcgccc aattcaatga
     2881 cacgatgaca ttgttgggct acataagcat cgtgggtcac aataatgact gttttccctt
     2941 ctcgattcat ctctaagaga aacttcaaga ccaaatctct attttcagga tccagcgaac
     3001 ctgtaggttc atcggctaaa atcagctggc tgggttttaa gatggctcta gcaactgcaa
     3061 ttcgttgttg ttcgccccca gacaactcgg agaccctttg atgcaaagta gctgacaaac
     3121 ctactctctc taaaatctct tccacctttt tgagtttgtc tttcttaggc aatttcacat
     3181 atttcagcgc cagcatgaga ttgtactcga ccgtttcatc atcaatcagg gcaaaatttt
     3241 gaaacagata agagatatgt tcacggatta ttgtttgcga cttagcagaa ttaaccgcta
     3301 gatttgtctg accaaaaatc tcataccgtc cgctataatc accatctatc aaacccaata
     3361 aatttaacaa ggtcgacttc ccactaccac tcttaccaac aatagctacc aaatccccct
     3421 gatcaatcct gagagataag ttatccaaaa tcacttttcc cccaatggtt ttggtaatat
     3481 ttttcaactc aatcataaga tgcccccttt caataactct actagacttc ttttctccat
     3541 cctagaagct aagcctagca caaatagtat atccagacat gtaaaacctg caaacagtag
     3601 aagtggtaag aacgcatggg caaagaaaat caagactaga agagggaaac tatagcccag
     3661 caagagcaga acgaggagag gacggtagcg atcgaccagt ttccacccca taaacttctt
     3721 ggtaatgata tccctgcgct tcaataagaa agttgttact agtaagaagt aggaaatcat
     3781 catgctaagg agaccaaaca aagcaaagag taggttaaaa ttccgaacag catctcgata
     3841 agaatccact ttctcttgtt gaatggcttg aatagatgaa aattttaaat aatttccatc
     3901 tgacaatttc tcaactaact ctgtaatctc tttttgatgt tgaaccgtat tttcaatttt
     3961 aatcggatta tttaagccag ttgttgacag ggaggctttc tcatcccaca tcatatcaga
     4021 atcattgacc aagctaataa ttggattgga gagattttcc tttcgcttat cactatatgg
     4081 gaaaaatgac caatctcctt cataataggc aatctcgaca tccatctcct ctatcgttcg
     4141 tttttgctgc tcttcatact tcatcgaatg aaaggcaatt aacttcccca agagctgatt
     4201 tttatcttct tcacctttcg tacttgctgg catcaaaata acttttttaa taccggtatt
     4261 tggtagcttg aatcccttgc tctttagaaa attgcgattg gcatagtaaa catccaccgt
     4321 atctgttaac tgatattgct gaatctgttc tgattggaca aaatttttta caggaagact
     4381 gctactctgc acatagcccg cctgcgtttt ttctaccaaa tcctgataaa atcgatagaa
     4441 ataatctgta gatttccctg accctgctag ctcttcttgc cacagattat cattgagttt
     4501 gaaggtttct aaggtcaggt aattaccttg acttacccac tgttgctgat aagcaagttc
     4561 tttgttttct tgttctaaac ttctgcccac cccaatcagt aaggccgtca gtaaaatagt
     4621 tgtccctatt ttcatcacat aattgaagat aagaccaaat ttgaaagatg aaaaaccttt
     4681 cagcagagag ctgattgtca ttttttggat taagaggtaa gtcaaccaac tgataaagag
     4741 ataaagctgc aacagcaaaa aatgagacaa ccacagcata ggaaacaaat cttttggctt
     4801 ataatcaagc aagaaaaaca cgcctagatt gatcacaaga gccccaccta ggaggaggta
     4861 aaggttgcct tttacaacat cagctaaaac agccctatct tgaaaaccaa gtaatttttg
     4921 taccccaact cttttcatct ccatcatcgg ttgatacact gtcactaaca caagaagcaa
     4981 aatagccaag acaaaaacaa tggcagataa aagcaaatct cgatttatga cttccactgc
     5041 acttttgtag gtcggctcta gcaaggtagc ctggtctatc ttgaaaaaat cgctccattt
     5101 ctgtacaatc ctatccttgt ccatctcttg tgtagaagtt atcgtatagc gaccatttaa
     5161 actacgagat gtatccttga tataggtttg aaaagtcata agctgaatag gtttggcttt
     5221 tagaaaggtc ggaatcgtac caagtttatt ggaaatttct ttattactat agactccttc
     5281 accatctgtg gtaaaatcaa gagaagaaat cccaaactct tggtagggga aggtatcttt
     5341 atcaaaaaca ccagacttga ccacctcatc accactgtct gttttgatga tggagacttt
     5401 atactccttt gatacatcct caaaaaatcg aagaacagac gctgcaggtt cgttaatatc
     5461 tttcaaatac aaatccaaag aatctacagt ctttcccatt tcttgaatcc gaaccacttc
     5521 tcgtgtatag tttacattaa aaacaaaaaa agtggtacac agaagcagga gtaacataca
     5581 gaaattactg atttttttca taaaagtgcc tccactacag ttttccctct ccaaatagga
     5641 gagggaagaa ttttactcaa atccatagaa atattccatt cctgttggcg gaatcttggt
     5701 gagggaagct ctagcccatg catcaggttt tgcataatcc ttagaggttc tacccccatg
     5761 tctaacagtt gctgtatgat atcgagtaga atgtaagtaa tcagaatatc caaaagttcc
     5821 tgtccaacct actccatagt tccattgacc accctcaacc catactgcta aagcatttgt
     5881 agtagcaagt ataccacata ccattaaaga tgcaacacca attttaacta attttctcat
     5941 gatttatttc tcctattatc gagagctgtt ccgtaaaaac agctatagta atttatcaac
     6001 aagtatcgta gtccattgga ctcaccttcc ttctatctca tttcattttt taacaacgaa
     6061 cttactctta accgggagaa tggtgcgctt ctgtatcagg tccttgaaca accagagtag
     6121 tattaagcaa actctaacta ttactcaaga atcagtccaa taaacaatat ggatagcgct
     6181 ttcaaatttt taaactccta tcccgtcctt ttatctatat aataagcata agcgaaaata
     6241 taataactgt caagtatctg aagtgaattt tataaaaaaa ttacaagcca aatttgtaaa
     6301 gtttacacta agccgctagg gcaatcgtct atcagaatgt ccgtttattt gtcaataatc
     6361 cgagaaaatc ttgcaacgct tagaagtcta taaaaactat caacgtttat atgacttgcg
     6421 aatagcaatc ctgctcaacc tttccacact ctatctatac aatcaagata aaaacatgtg
     6481 taagcaaatc tgctacactt tactggagga cgccaagaat aagaaaagct acgataggct
     6541 ggctatctgc tatgtccgta ttgggatttg tacagataat gctaaactta tccaaaaagg
     6601 gttctccctt ctggagctaa ccgaggaaac ttctatgctg tctcatctca aaaaagaagt
     6661 agagatctat taccaagcga aggaaagata aaaaagccga gggatttcct cgactcttta
     6721 catattattt gttcgttagt tcttaatacc agccattgtt aagccagaag tttttagcag
     6781 cagtccatga accgtaacgt cctgcaacgt aggcatctgc tacacgttct tggttttcag
     6841 ctgagtagtc accgttcagg tatgaatctg ttaattggta acgtccgata taacgtccat
     6901 ttgtagctgt atagctacca cctgattctt tttgagcgat ccattctttg gcttctgctt
     6961 cagatccgct tacagttgaa acaactgttt cacttactac tggagtttct gctactggag
     7021 ctgaaacagt ttcatcttga gcggctggtg ccgcataagt agctggcgct ggtgttgcaa
     7081 caggcgctac agggccatcg ataaccaact cttgaccaac ataaatcaaa tgaatgttat
     7141 caatgtggtt gttttctgcc aatttttcaa ctgttgtgtt gtgagtttca gcgatttctg
     7201 aaagtgtatc accttcttta acagtgtaag ttgatgattc ttgagcagat acaaatgatg
     7261 gagcaaatac tgcaaacaag gcagctactc ctgcaagagt tgctttaatc tttttagtta
     7321 ttgatttcat attcgaaaat tctccttctt tctatagttc tatcataccc tttaaatatt
     7381 accgtttctt gacactttta tgtagaaata ttacaaaatt attttttatt tccctaaata
     7441 aggtgttttt tgtcataaaa gatactattt ttatgctatt tgaggtatta gaaaggtttt
     7501 catccacaat taaagccaag cccctcattc tttgatataa tagagataag aatttttata
     7561 aggggaaact gatgaaatca cttcgttttc aatctgtctt tgatatcatc ggaccagtta
     7621 tgattggccc atctagtagc cataccgctg gtgctgttcg tattgggaag attgtctctt
     7681 ccatttttga tgatactccg acagaagttg aattccaact ttttaactca tttgccaaga
     7741 cctatcgtgg tcacggaaca gacctagccc ttgttgcagg tattttagga atggatacag
     7801 atgatcctga aattccaaat agtctggaaa ttgcccacaa gcgtggtatc aagattgtct
     7861 ggaccattca gaaagacagc aatgcccctc accctaacac cactaaaatt actgttaaaa
     7921 atgcccacaa gaccatcagc gtgactggta tttctatcgg tggaggaaat attcaggtaa
     7981 ccgaactcaa tggttttgcc gtctctctca atatgaatac accgactatc atcatcgttc
     8041 atcaagatat tccaggtatg attgcccttg ttacagaggc gctttcccgc tatggtatca
     8101 atatcgccca gatgaatgtc actcgtgaaa aagctggtga aaaagccatt atgattatcg
     8161 aagttgacag tcgcaactgt gatgaggcta tcgaagaaat tcgaaaaatc cctcatctcc
     8221 acaatgtcaa tttctttaaa taggaggaag catgttttat tctatcaaag aattggtcga
     8281 gcaagcagat ctggactttc aaggaaatgt cgcagaactc atgattacaa cagagtttga
     8341 attgaccggt cgcgaacgtg aagaagtctt ccttctcatg gaacgcaatc tggaagttat
     8401 gaaagcctct gtccaacttg gcctcaatga aaataaatct cgtagtggcc tgacaggtgg
     8461 agatgctgcc aaattggacc actacatcaa aaacgaaaaa actttatcag attacacgat
     8521 tctctctgct gcccgaaatg ctatcgcagt caatgaacac aatgctaaaa tgggcttggt
     8581 ctgcgctact cctaccgcag gaagtgccgg ttgtctccct tccgttctca ctgctgctat
     8641 tgaaaaatta gacctcagcc acgagcaaca attggatttc ctctttgctg ctggtgcctt
     8701 tggactagtc atcgcaaaca atgcctccat ctcaggtgct gagggtgggt gtcaagctga
     8761 agttggttca gcctctgcta tgagtgctgc cgccttgact ctggctgcag gtggaacacc
     8821 ttatcaggcc agtcaagcta ttgcctttgt cattaaaaat atgctaggcc tcatctgtga
     8881 ccctgttgca ggtttggtcg aagttccctg tgtcaaacgt aatgccatgg gagctagctt
     8941 tgctttcatc gcagcagaca tggccttggc aggtatcgaa tctaaaatcc ctgtggatga
     9001 agtgatcgat gccatgtacc aagtaggagc aagcatgcca actgcctttc gtgaaacagc
     9061 tgaaggtgga ctcgctacca cccctactgg tcgtcgcctc caaaaagaaa ttttcggaga
     9121 ataagcttat caaataggag aaaccatgac ctctatcaca gcgatttttt tcgatttgga
     9181 tggaaccctc gttgatagtt ctatcgggat tcacaatgcc tttacctata cctttaaaga
     9241 gctgggggtg cctagccctg atgctaaaac tattcgtggt tttatgggac cacctctcga
     9301 aagtagtttt gcgacctgcc tgtccaaaga ccaaatttct gaagccgtgc agatatatcg
     9361 ttcttactat aaggcaaaag gcatctatga agctcaactc tttcctcaga ttatagactt
     9421 gcttgaggag ttatcgagca gttatccact ctacatcacc acgacaaaga atacatctac
     9481 tgctcaagac atggcaaaaa acttggaaat ccatcatttc tttgatggca tttatggttc
     9541 tagccctgaa gcaccccata aggcagatgt cattcaccaa gccttgcaga cacatcaact
     9601 agtccctcaa caagccatca tcatcggaga taccaagttt gatatgctgg gagctcgaga
     9661 aacaggtatt cagaaattgg ccgtcacttg gggatttgga gagcaagcag atctactaaa
     9721 ctatcaacct gattatatcg ctcacaaacc cttagaggtt ttggcgtatt ttcaatagca
     9781 tagactgggt aacaattcca tttcagaaga aaatgaagta atactcaatg aaaatcaaag
     9841 agcaaactag gaaactagct gcgggctgct ctaaacactg ttttaaggtt gtagatggaa
     9901 ctgacgaagt cagctcaaaa cactgttttg aggttgtgga tagaactgac gaagtcagta
     9961 accataccta cggcaaggcg acgttgacgt ggtttgaaga gattttcgaa gagtataagt
    10021 ctctacataa taaaacgcat attaccaagg ttgtgtaact tgatactatg cgtttttctt
    10081 ttttattcaa tgtgggttgt ttgattagca aaggcgataa tagcttgctt caactcatct
    10141 ccactgagag tgcggaactc tccaatcttt tgattgatgg attctaaagg catgacatta
    10201 accttaccaa cgaaaatctt atccataact tgattatcaa cgagttcggt tgataccaag
    10261 agttcttcgt agagtttttc acctgggcgg attccaactt caacgattgg aatttcactt
    10321 tcagtgtggc cacttagaag caccatcttc ttggccaagt cataaatctt gactggtttg
    10381 cccatatcaa ggataaagac ttccccatct ttggcataag caccagcatg gataaccaga
    10441 cgactagctt ctggaatggt cataaagtaa cgggtcatgc ggaagtctgt caccgttaca
    10501 ggcccacctt cagcaatctg acgttcaaag actggaatga cactaccacg gctaccaaga
    10561 acattcccaa aacgaactgc acagtaggtt gattggctac gttggttaaa gccagtgaca
    10621 atcaactccg ccacgcgctt ggttgctccc ataacatttg gtggattgac tgccttatct
    10681 gtcgaaatca taaccatctt agacacttta gcttcatcaa cagccttagc aacattgtaa
    10741 gttccacgga tattgttttt gaaggcttct tttggattgc gctccatcat aggaacatgc
    10801 ttgtgggcag ccgcatgata aacaatagca ggtttgtact gctcaaagac ttgcaacaaa
    10861 cgatcatagt cttgaatgtc cgcaatcaca ggtacataat caatcccttg gaacttacga
    10921 atcaattcat gataaacaag gtagattgag ttttccccat gaccgagcaa gacaatgcgt
    10981 tcaggattga agcgactaac ttgacgacag atttcagaac cgattgaacc tccagctcct
    11041 gtgactaaga tggtcttacc tgtcagttct gcacccagac gcgattcgtc aagacggatt
    11101 tcctgacgac ccaaaaggtc cgtaatatca attttttgga agccagtacc tgcttggtga
    11161 aggccctgaa caacagtttc aaccttaggc atcttgtaac atttgacacc cagcttatta
    11221 cacatctgca agatacgctc atattctgac ggatccagcg acggaatcgc aacgatgaca
    11281 cgctcgattt gatggcgttt ggctaattca ggcagattgt cataagagcc caaaacagga
    11341 ataccaccaa gtttttgacc ctttttctta gaatccttat ccaaaatacc gaccagttct
    11401 aattcactgg ttggatgttg gtaactatcc ataaagagag ccccaccatc accggcacca
    11461 atcaagaagg tccgacggtg ttctccatca ccactacctt ttttgcgtct ggagtagatt
    11521 aactgccaag taatccgtgg caataaaatc aagaaggtac tcaacaagat aaagagaatg
    11581 atgaaacgga tggagaagag tggcaagaag gcataacaga tactatatga caagacactg
    11641 ctagcagtca caccaaaaaa gattttcatg aaatccgtaa tcttgctgta acgactaatg
    11701 ctcgcgttca acccccaaaa accaatcatc aattgataga acaagaaggc caaactcgta
    11761 tagataatgt agtcaacagg tgctggatta atcagcccat aaaataaaat ataagataca
    11821 atgatggaaa ccaccatact gaaaatatca aatattcccc aaaaaacctg cttttgttgt
    11881 ttatttaaaa tttccaccag atcaatcaca taatctgtta gttttttatt catagaaatc
    11941 tactcctcct taaaagagtt ttactatatt gttattgtag catattttga tttatttggc
    12001 ttattttatc caaataaatt attgttctct tgtactcctt cttccattta aatcctctaa
    12061 ttaaacctac aatcgtccca aggccataag caaagtgaat ggaaaataaa agaaagggca
    12121 tcacaattag aaatccattt ttatgtttta ataaagtcag caaagtgagt aatgacaaaa
    12181 gtagaaaata ggcacctaat agtaaagtta tgaatacgaa tgtgatcggt aacaatgcta
    12241 gactaaacac aagactcaaa acaaataaac aaggaacata gtgaaataat gataaacact
    12301 taaactgaac atgacttgtc aagccaatcc acaaaccatt tgaatacttt tgatgcagca
    12361 ttttcttgaa tgttggtcga atatactgat aagatagaat acttgggcta tagcggattt
    12421 tataaccata ttctcgaatt ctataatgaa tatcattatc ttcagttcgg ccaagttgct
    12481 catttactaa accaaccttc tggaaaacct ctcgtttata cattccatga aaaatagaag
    12541 aaacatatct atcctcagaa ctatttcgat aattggcaat gctactgcca aacatatttt
    12601 cctcaacaag atgcaaggtc tctgcccatt ttccttttcc ttcgacaatc gtcggtctag
    12661 gccccccaca gacaaattca ccttgttgaa taatagccac attgttcatt acaaaagtct
    12721 cagtaacttt tgaatgagca tcaattttta aaataaggtc ccctacagaa tgtttaactc
    12781 ccaggttaaa accactagct tgatttttct taggattgtt atacaatcta attgagttaa
    12841 actctgtatc ttcctttata aattgctgaa tgatagctgt ggtcccatct gtggacatag
    12901 catttataaa tagaatttca atattctctt taggataggt ttgatttttt aagtcttcaa
    12961 ttagaccagg cagatatttt tcttcattgt atgcactgat tacaatggat acgagaagac
    13021 tttcaacatt ttgagtcact actactctcc tgaatttttc cttccaccat tctatcataa
    13081 ttttcaagac tttcccattt ttatcttgaa agccagaaac cttacttgac attatagtga
    13141 aaaagcctta aaataagctg ttattaaaat cagctagaag aggtattata tgaaaaagca
    13201 atcactcttt tttgttccag gtattatcct gattggtgtt tccttgcgaa ctccttttac
    13261 tgttttaccc attattttgg gaaatatttc gcaaggtctg gaggtagaag ttagttcgct
    13321 tggtgtcttg accagcctgc ctctccttat gtttaccctc ttttcaccat tttctaccca
    13381 actggctcag aaaatcggct tggagcatct cttcacctac agcctcttct tcttgaccat
    13441 cggctcactt attcgactaa tcaatctgcc cctgctctat ctaggaacct tgatgattgg
    13501 ggcaagtgtc gcagtcatca atgtcctgct tcctagtctt atccaagcca atcaaccaaa
    13561 gaaaattggt tttctgacca ccttatatgt aacgtctatg gggattgcaa cggctctggc
    13621 ttcctatcta gctgtgccca ttacacaagc cagttcttgg aaaggactta tcctcctcct
    13681 cacgttactc tgtctagcaa cttttttggt ctggctccca aatcaccgct ataatcatag
    13741 actagctcca caaaccaaac aaaaaagtca aataaaggtc atgcgtaata aacaggtttg
    13801 ggcaattatt atcttttcag gttttcaatc cttgatcttt tacaccgcca tgacctggtt
    13861 acctaccatg tctatccatg caggtctatc cagtcacgaa gctggcttgc tgacttctat
    13921 cttatctctg attagcattc ctttttcaat gaccatccca agcctgacaa ccagtttatc
    13981 tactcgcaac cgtcagctca tgctcactct ggtttcacta gctggtgtgg tcggcatttc
    14041 catgctcttt ttcccaatca ataatttcat ttactggctt gccatccatc tcctcatcgg
    14101 aaccgcaacc agcgccctct tcccttatct catggtcaac ttttcactca agacaagcgc
    14161 ccctgaaaag acagcccaat tgtccggtct atctcaaaca ggaggctata tcctagcagc
    14221 ctttgggcca accctctttg gttacagttt tgacctgttc cactcttggg taccagctgt
    14281 agctgccctc ttgctcatcg atatcctgat gactgtggcc ctctttacag tggacagagc
    14341 ggataagatc ctttaaactt ctagttttag ctagaagttt ttttatatat aaaaatttta
    14401 cacatttctc ttgacagggc tttcttttag atgtacaatg tatgtgtaga aaaattatat
    14461 ataaaaatct tacacattag aaaaggaggt ttcccatgta ctttccaaca tcctctgcct
    14521 tgattgaatt tctcatcttg gccgtactgg agcagggtga ttcttatggt tatgagatta
    14581 gccaaaccat taagctgatc gctaatatca aagaatccac actctatccc attctcaaaa
    14641 aattggaagg caatagcttt ctgacaacct attctagaga gttccaaggt cgcatgcgca
    14701 aatactactc cttgacaaac ggtggtatag agcagctctt gaccctaaaa gatgaatggg
    14761 cactctatac agacaccatc aatggcatca tagaagggag tatccgccat gacaagaact
    14821 gaatacctga ctcagctaga actctatctc aagaaactac ctgaagctga ccgtatcgaa
    14881 gccatggact atttcagaga gctctttgac gatgctggaa tcgaaggaga agaagaactc
    14941 atcgctagtt tgggaactcc caaagaagcg gcccacgaag ttctatccaa tcttctcgat
    15001 aaaaaaatca atgaagcacc cgctcaaaaa aataaccgac aaattttaca tatcgccttg
    15061 ttagccctcc ttgcagcacc tatcggcatt cctctgggaa tcgccatcct cgtgaccctg
    15121 ttcgcaatcc ttgtagccgc tttgactgtc attctggctt tctttgcagt ttccatactg
    15181 ggtatcatcg gcggattcct atttttagtt gaaagtttca ctgtcctcgc ccaagccaaa
    15241 tcagccttta tcttgatttt tggttctggt ttactggcta tcggtgcttc ttcgctagtt
    15301 ttacttggca tttcctatgt aactcgcttc ttcggtctac tcattgttcg tctggtacaa
    15361 tttgttctta aaaaaggaaa gagaggtaat cagcatgcgt aaatggacaa aaggatttct
    15421 catctttggt gtggtgacta ccgttatcgg ctttatcctg ctttttgtag gtatccaata
    15481 tgacgggatt aagagcctac tttccatgtc caaagaacct gtctatgata gccgtacgga
    15541 aaagctaacc tttggcaagg aagtcgaaaa cctagaaatt actctccacc aacacacgct
    15601 gaccatcaca gactctttcg atgatcaaat ccacatttct taccatccat ctctttctgc
    15661 tcaccatgat cttatcacca atcagaacga tagaactctg agtctcactg ataagaaact
    15721 gtctgaaact ccgtttctct cttctggaat tggtgggatt cttcatatcg caagtagcta
    15781 ctctagtcgt tttgaagaag ttattctccg actaccaaaa gggagaactc taaaagggat
    15841 caacatctca gccaatcgcg gacaaaccac catcataaat gctagccttg aaaatgcgac
    15901 cctcaataca aacagctata tcctccgaat tgaaggaagt cgtatcaaaa acagtaaact
    15961 cacaacgccc aatatcgtta atatctttga tacagttctt acagatagtc agctagagtc
    16021 aacagagaat cacttccacg ctgaaaatat ccaagtccat ggcaaggttg aactgactgc
    16081 caaagattat ctcagaatca tcctagacca gaaagaaagc caacgaatta actgggacat
    16141 ctcaagcaac tatggttcta tcttccaatt cacaagagaa aagcctgaat caagaggtac
    16201 ggaattaagc aacccttaca aaactgaaaa aaccgatgtc aaggatcaac tcattgcgag
    16261 atctgatgat aatattgatc taatatccac accaagcaga cgttgacaaa aacgcttaca
    16321 tctgctacta tggaagcata tttatctaac caaaaggagg ttctaccatg aaacaagaat
    16381 ggtttgaaag taatgatttt gtaaaaacaa caagcaagaa caagcctgaa gagcaagctc
    16441 aagaggttgc agacaaggct gaagaaacga tagccgatct cgatacacca attgaaaaaa
    16501 atactcagtt agaggaggaa gtctctcaag ctgaagtcga attggaaagc cagcaagaag
    16561 agaaaattga agctcctgaa gacagtgaag cgagaacaga aatagaagaa aagaaggcat
    16621 ctaattctac tgaagaagag ccagaccttt ctaaagaaac agaaaaagtc actatagctg
    16681 aagagagcca agaagctctt cctcagcaaa aagcaactac gaaagagcca cttcttatca
    16741 gtaaatcttt agaaagtcct tatatcccct accaagctcc aaaatctagg gataaatgga
    16801 aagagcaagt gcttgatttt tggtcttggc tagtggaagc gatcaaatct cctacaagta
    16861 agtttgaaac aagtatcaca cacagttaca cagcctttct cttgctcatt ctgttttctg
    16921 catcttcctt tttctttagt atctatcaca tcaaacatgc ttactatgga catatagcaa
    16981 gcattaacag tcgcttccct gagcagctag ctcctttaac tcttttttct atcatctcta
    17041 tcctagtagc gacaacactc ttcttctttt cattcctctt gggtagtttc gttgtgagac
    17101 gatttatcca ccaggaaaag gactggacgc tagacaaggt tctccaacaa tatagtcaac
    17161 tcttggcaat tccaatcttc ctcactgcta ttgctagttt ctttgctttc tttgatagcc
    17221 tacgatttac agctctcttg tgtgtgatta gcattggaat cattctgctt gccagtctcc
    17281 atatcattac aagaccaagt caagcaagtg aaaccgactc cttctatcaa ttattcttgt
    17341 ctgtccttgt gaacggagtt attatcctcc tcttctttgt agctgaagtg gcactgattg
    17401 gagattatct tcgtatcttg gcctttcttt aaaattcaat cctgtcatcc ttagcaggat
    17461 tttttctatg tcaaaaaagc aagtcgattg acctgcttct gctttacttt tcttgtgcta
    17521 atgttttaaa atcttgtcct aggatgggtt gtatttctat ttgattggcg tctagttggt
    17581 ggtaaaaaga taggggtaat acctctaaga attttgcata gtccaagcgg gcaatggctt
    17641 catagtcttc tggtttagag ccatctcctg tgatttgaac caagtcaatc tcccactgga
    17701 cttccttatc caccaattgc tcaatatagg ctgcaggaac aaacggttct ctcggtaaaa
    17761 ctgtcttgac tccttgagct agatggtaaa cgccatgcac ttttccttct ccatcctgat
    17821 agaaggaaac tctcgcaaag taggcatctg tctcttgaac cgcacgcaca cgcgcttcaa
    17881 actgagccaa gccatctgcc tctaaaaagc ttttcaagtt ctcacgacta aagaaaatag
    17941 gattaaaaat tccttggcaa atcgtaaaac gagctacagg gtctgctaga taggcttgaa
    18001 tacttgcttg gaaataagct tcaaaatcaa aaccatctag cccttcaata tcagtccctg
    18061 tataagcaag caaagcatct aaaaatgact tgataatctg ccagtctgtc tttgttagta
    18121 ggtcaggaag atcgacacgg taagcctttt gaccatcagc ataactgacc ttaaagagaa
    18181 attgtgattg ccccactatc gcacactcga tatattcgag acgattaaga ggctgacgga
    18241 gatagactac atcatagcta tgtgactcca aaccgtctag caaggccaag atcgacttgg
    18301 cagtcaaaat ctcttgttct cctaaaatgc tctgtttatt tggaataaaa aatgttttcg
    18361 ccataactgg actcctttga aatcgtttac atacagtata ccttattttt ctgaaagata
    18421 caaaaacaaa ctttagattt ttgagtacaa agcatagaaa aacagcccct gaggactgtt
    18481 tgatcttata cagtagctgc ttgatccaag ctttcaccga tagcggctag gcgctcgatc
    18541 acttcagctt gtgtcaattc attttttgaa acatagcggt tacgtgggtg aacacggcac
    18601 tcgtgtgagc atccacgaag gtacttgtct tcattttctt ctgatgtcaa gatacgacgg
    18661 ttacagaagg gatttccaca gttgacataa cgttcacatg gtgttccatc aaaccagtct
    18721 ttccctacga tagttgggtt gacatggttg acatcaacgg caatacgctc gtcaaagacg
    18781 tacattttcc catcccaaag ctcaccttga acttctgggt ctttaccgta agttgcgatt
    18841 cctccgtgca attggccgac atctttgtaa ccttcacgaa ccatccagcc tgagaatttc
    18901 tcacagcgaa cgccacctgt acagtaaacc acgacacgct tgtccatgaa tttttccttg
    18961 ttatcacgga cccattgtgg taactcacgg aagttgcgaa tatctgggcg aatagctcca
    19021 cggaaatgtc ctaggtcgta ctcataatcg ttacgtgtgt caaggacaac ggtatcttta
    19081 tcaagaagcg cttctttgaa ctcttttgga gacaagtaag cacctgttgt ttcaagtggg
    19141 ttgatgtcat tgtcaaagtc gttgtcttcc aaaccaaggt ggacaatttc tttcttgtag
    19201 cgaacaaaca tcttcttgaa ggcttgttca ttttcttcgt caatcttgaa ccagagttct
    19261 tccattcctg gaaggctgtg aacgtagtcc atgtattttt gagttgtttc atagtcacct
    19321 gaaactgttc cgttaattcc ctcgtcagcg actaggatac ggcctttaag gccgattgat
    19381 ttacagaaag ccaagtggtc tgcagcaaat tgctctgcat tttcaattgg agtataaagg
    19441 tagtaaagta agacacgaat atcttttgcc ataagatttg ttctcttttc tattcttaaa
    19501 ttttctaaat tttttattta tctacactag tatacctgct tgagaaaacg aatgcaattt
    19561 atttgactgg aaattgtaga gggttctcga aatttcctga atggttaaaa taaggacaag
    19621 agaaaacatg gatatctata tccttgtgcc aaaaaaacca ctgccctccc cagaccaacc
    19681 tgaggaaagc agtgattctt attttaggag ttaggaatga atacacgaaa tcaatttagc
    19741 tgattatttt ttgtttttca agaattcatc gtattgtttt tgcatttcgt tcaatacttt
    19801 ttcgtaggca ccttcagatt tcaatttttc catcaattct ggaatcgctt tatctgggtc
    19861 tacagtacca gtgttgatag ctgtatcaaa ttgttgcatt gtgttagcaa tagctgagat
    19921 ttcagatttc acattgtcag tattgaagat aaatccaagc gctggagatt ctttagcttc
    19981 tgccaattct ttcttagaat tttcgatttg ttggtctgta acgttttcgt tgatgtaaag
    20041 gatccagttg ttaccagtgt tccatccacc catgtgagtg tttcctttgt agccatcaag
    20101 aacgcgaaca cggttttctt taccttcaat tttttcccag ttcttgcctt ctggaccgta
    20161 aacaagaccg ttcaagagtt ctggattcgt attcaagagg ttcaagattt ccattgattt
    20221 ttctttgttc ttagagttgt ttgagatgac aaagttagca acttgtgttg tttggttttt
    20281 cttgatgaag ttagtaattg gtttgatttg gatatctttg ttggcaacac gtgaaagcaa
    20341 gctgttaccg tagtcagctg gtcctactgt ttcttcacga acgaaccaag tatcttgttg
    20401 aaggtcaaag gaagtatcgc ttgttgcgac gtcttttgga atgtagccag cttcatagaa
    20461 tttgtgaaga gtcttcaagt gttctttgaa acgaggcact tcgtaacggt ttacaacttt
    20521 agtagtatcg ccttcaaggt cgataacgaa tggaagaccg tttgctactg ggtagtcaaa
    20581 gttatcagat gggatgaaaa ctttaccaat agcaaatggt actacgtctg gagctttttc
    20641 tttgatttgt ttcaagactg gctcaagagt ttcgtaagaa gtaacacctg aaatatcgat
    20701 accatattta gcaaggagag ttccgttgaa ggcaaagttt tgagatgatg caacgttggc
    20761 tgcaactgga acagcgtaaa tcttaccatt tacagtatta cccttgatgt aagctgggtc
    20821 aagtgctttg taaaggtctt taccttcttt tttgtacaat tctgtcaagt cagcgtaagc
    20881 acctttttga gcatttacaa tatagttatc tgcaaaggca atatcatagt tttcaccaga
    20941 tgatgtgata actgacattt tcttaccata gtcaccccag ccaaggtatt ggatatccaa
    21001 tttggcacca actttttctt cgatgatttt gttggcattt gctaacaatt catccaagtt
    21061 atctggtttg tcaccgattt ggtacatttt tataacaggt ttgtcacctg aatcagcagc
    21121 ttttttgctg ttacctgtca aatttccaca agcagcaaga cctgcagcca aagcgactac
    21181 actagcagat gcaaaagcat attttttcca gtttttcatg ataaaaactc ctttttttat
    21241 ttttaaactt ataaacaatg taatgatctt atactcaatg aaaatcaaag agcaaactag
    21301 aaaactagcc gcaggttgct caaaacactg ttttgaggtt gtggataaga ctgacgaagt
    21361 cagttacata tatctacggt aaggcgaagc taacgcggtt tgaatttgat tttcgaagag
    21421 tattaacttc acacaaggga agttgggaac tgagaaatgt tatttctcaa taagcactat
    21481 tctttcacac caccgatagt caaacctttt acaaagtagc gttggaagaa tggatacaaa
    21541 atcgcgattg gaagggttgc aaccacaacc atggccatac gacctgtttc tttcggtaga
    21601 gcaactccca gttgaccagt caagccgacc gctttggcaa tgtagtccat attttgttgg
    21661 atttgcatga gcaaatattg caatggatac aagttgtcac tcttgatgta aagaagggcg
    21721 ttgaaccagt cattccagaa accaagagct gtcaaaagcg tgatggttgc gatacctggt
    21781 agtgacaatg gcaaacagat ttggaagaaa atccgggcct cactggcacc atcgatacga
    21841 gccgattcga gaatggcttc tggaatggtc ttcttgaaga aggaacgcat caagatgata
    21901 ttgaatggtg agagaagcat tggaacaatc aaggcccaaa cagtgtcacc aagctgaagt
    21961 acacgggtca ccatgatata acctggtacc aaaccagcgt tgaacaacat actgagaagg
    22021 acgaagatgg taaaaaatct gcgatactta aaggttgtcc gtgaaatagc gtaggcatag
    22081 gttgttgtga taaagacatt tgtcaatgtc ccaactacgg ttacaaagac agagatgaag
    22141 agggcttgta ggattttatc cttaaactgt gccaaaaact caaaaccgtc taagccaaat
    22201 tgggatggga agaagctata gccgtattgg aggaggcttt tctcgtctgt cactgaaata
    22261 atgataacga atacaaaagg taggatacaa gagagggcaa tcaaacccga aatgatactg
    22321 aagaagatat ctgctttctt actgaaggag tgaatgccga cattatcaat tttttctttt
    22381 ttaattttct tttttgccat attctcctcc tttctagaac aaagctgagt ttggatcgac
    22441 tcgtcttgca agcaagtttg ataggataac cagaatcaaa ccaacaacgg attggtaaag
    22501 accggctgct gcagccatac cgatatctgc tgtctgagtc aaaccattaa aaacatatac
    22561 gtccaaaacg ttggttacat tgtaaagctg accagcattg tgtgggattt gatagaagag
    22621 accgaagtct gcgcggaaga tatttccgac tgcaaggatg gtcaatacag ttacaagcgg
    22681 agtcaactga ggaatggtta cgttgcgaat acgttgccac ttgctagctc cgtccactgt
    22741 cgctgcttcg tagtaggttg gatcaattcc catgatcgtc gcatagtaca tgacactgct
    22801 atatccaaaa cctttccaaa tacctaggaa aagtaggaga tagggccaga tgcccaggtc
    22861 agcgtagaaa ttgacttctt tgagaccaag actttccaat agatgattga acaccccttt
    22921 atcaatattt aggaaggcat ctgtaaagaa actgatgata acccaagaca agaagtaagg
    22981 gaacaacata gaagtttgga aaatcttcac cattctctta gaacggagct cgctgaggat
    23041 aatggcaatc cctacagata caactaaacc tagaaagata aagccaagat tgtagaggac
    23101 agtatttcgt gtgataataa aggcgtctct tgaactaaat aagaatctga aattatcgag
    23161 tccgacccat ttactattta tgatactatc tatgaaacca ttactggtca tgtggtagtc
    23221 tttgaaggca accacgttcc caaatactgg aatgtaaaag aatagaatca accagagtgc
    23281 ccctggcaaa accatcaaga gaaagatcca gttgtctctc aatgtttttg aaaacttttt
    23341 cataatttcc tcccttttta ttttgatatc catctaaaaa ttctttttta gacttttgat
    23401 aacgattaca ttattagtat actcctattt gcaggttagg ttaaactcct aattatagaa
    23461 aaaactccac aaattatgta gcagatttaa aactttatca ccactatcaa acaaatgtcc
    23521 taaatcaatt gtttatttta tctctattag cccagtgatg gcgtcactct gttataagca
    23581 tccaacaacg gggtatactg aaaaatctcc agactaggga actcagcgat agttcctaat
    23641 ctggagattt ttaatatgtt attaggcgtt tgctttcaac ttagcaataa cctctttaag
    23701 attatcaatc aactctgctt cagtatgctc agagcctttt tcatctgcca agaacaaaac
    23761 tgctttttga agttcttctt gagagttttc aaggacatcc ttatctgctg tttctttgcc
    23821 cgctgtttgt cctccatcct tagttgctga ctggctttcc ttaatggact ctagggaagc
    23881 aatggcatct ttgactgttt gcaagatatc acgtaaacct tgctctgtca aactatcatc
    23941 tgtaagagct ttcttagcct ctgccaaaac cagacgtgct gaatctgtgg taggattcga
    24001 tacacctgtc aatgatctca aaagattttc taaggtttga gtctgcttac taatactaga
    24061 ctaaaatcaa aaagcattac ataacagtga tatgaaatca actaaagaag aaatccaaac
    24121 catcaaaaca cttttaaaag actctcgtac agctaaatat cataaacgcc ttcaaatcgt
    24181 tctatagtaa aatgaaacaa gaacaggaca aatagatcag gacagtcaaa tcgatttcta
    24241 acaatgtttt agaagtagat gtgtactgtt ctagtttcaa tccattatat ttcgtctgat
    24301 gggcaaatct tataaagaga ttatagagct tttatagtga tttgaaataa gatgtgaaca
    24361 actctatcag gaaagtcaaa ttaatttata gaaatatttt aacagccaag gtgtactgtt
    24421 ataaattcaa tacactatac ttggtggttt agctctcctg ttttctcttt tagctttaac
    24481 cagccataaa tggtattacg tgagatttga aaaacgtgtg atgcttctgt tatactacct
    24541 gttcgctcac aataagagag aactttttta cgaaaatcta ttgaatatgc cataaaagat
    24601 tataccacat tgtgtactat ttttggttca ttttactata tcactttgga ctatatcggt
    24661 ctagttatgt ctaaacgatt atagctggat actaagagaa atcaaaaaag cactaaggga
    24721 gaacgtccca aagtgcttaa tatcaaggcc tcaaagccta tcttatttaa taaaatatta
    24781 caacattttg ttgtagaatt caacgacaag tgcttcgttg atttctgggt tgatttcgtc
    24841 gcgttctggc aagcgagtca atgaaccttc caacttttca gcgtcgaatg atacgaatgc
    24901 tggacgtcca agagtagctt ctactgcttc aaggattgct ggaactttca atgatttttc
    24961 acgaactgag atcacttgac ctggagttac gcggtatgat gggatatcaa cgcgtttccc
    25021 gtcaacaagg atgtgaccgt ggtttacgaa ttgacgagct tgacgacgag tagtcgcgag
    25081 accaagacgg taaacaacgt tatccaaacg acgttccaaa agaagcataa agttgaaacc
    25141 taggattccg cctttgattt ttgtagcttg tacgaacaag ttacggaatt gtttttcacc
    25201 tacaccgtaa gtgaaacgaa gtttttgttt ttcagccaat tgcaaaccgt attctgacaa
    25261 tttagaacgg ttgtttggtc cgtgttgtcc tggtacgtag ttacgacgtg ccaattcttt
    25321 acctgtacct gtaagtgaaa ggccaaggcg acgagcttgt ttccaagatg gtcctgtata
    25381 acgtgacata tgaatgtcct cctgatataa ataatatttc ggtggaaata gtcacttaga
    25441 aagccctgat tcgtgcagat gcccttcgcc taaacagcca aggttacttg tcataagaca
    25501 cctgttgacg agcttcatgc tttcctgctg atatttcaca caaaatctat tctaccatga
    25561 atagctggat ttgtaaaggg gttttaggct ttattttcac taccagagag gttgagaacg
    25621 agggtaaagg tacttcctag accgtactgg ctgctgactg tgatttcccc acccaattga
    25681 tgggccaatt cacgcgcaat cgcaagtcct aatccatgac cacctgtctt catgttacgc
    25741 gaagtttcga cacgataaag acgtttgaaa atattttcca aatcctctgg ggcaataccc
    25801 tgcccttcat cggtcacact gattgaaagc tggtccttct ccagcttagc caccacttcc
    25861 agcttggttc ctggagcaga atatttaaaa gcgttatcga ccagattcac caagatacga
    25921 gaaagcttag cataatctcc ctcaatccgg gcagactctg ggattacctg caagtggaca
    25981 tctcttctct cctgctcaat caaaaactga aattcactca tgcactcaat taagagcttg
    26041 tccagaaaaa tactgtcttt actggtagtt tccacctgat ttctagctgt gtttagggtc
    26101 aaaaaattca actcctcaac cagtttattg agcctctccg tctggcgtcc aatggttgct
    26161 agataatgag cttgctccga ctccttgata atcccatcca aaatcccttc taccgtcgct
    26221 tgaatcgaag tgataggggt cttaatatca tgcgacaact gggcaatcat caagcccttt
    26281 tctcgttcgc tttcttccaa ggaatcaaag cttacctgca aatcatggga catctcatta
    26341 aaagtttgcc ctaattgctg aaattctaca ggaccttgaa cctccaaatt tgaaggaaaa
    26401 tccttggccg ctacccgctt ggcatgctcc ttgagtttgc ccaacgacgt aaagactggc
    26461 aataggagaa agagactaat cccagcaccg acaaggctgg caacgatggt catcccaagc
    26521 aaaaagtaaa tctcgccttt cgcaatcagc attttttgaa cagcccaaaa aacaaccaaa
    26581 atggttaaga gggttgaaat aatatatcca accaaaatat aactttttag tttcatgttt
    26641 gtcctctcgg tttctctatc ttatatccca acccccaaac tgtcttaata gtgggcgttt
    26701 ggtcactact atattttgcc agctcctgtc gaagagcatg gatatgcaca ttcaaggtat
    26761 tggtgtcatc cacgtagtct tctttccaga tcttttcata gaggtctgtc ttggagaaaa
    26821 ctcgctctgg attgctagct aaaatccaca gcaattcaaa tgatttaaca gttaaatcca
    26881 gcatttcttc tcctatttga acttcatgac tactatgatt catttttaga ttgccaaggg
    26941 aaatcagctc tgtttcgccc ccacgatgaa ggcggcgcaa aatattgtgg acacgcaaaa
    27001 ctagctcacg tgggctaaaa ggcttagcaa taaaatcatc tgctcccaag ctcaggccgt
    27061 aaatcttgtc ctgttcactg gtcttagcag taataaatag gaaaggctgc tctggtgata
    27121 agtattgaac ctcactgatt aaatcataac catccatccg aggcatcatg acatctgtga
    27181 taatcaaatc aatcggtttt ttcttgaaca gctctagcgc ttccagtcca tcatgggcta
    27241 ccaagacctg ataacctgcc tgaattaagt atctctgatg aatatctgtg atttctacct
    27301 cgtcgtcaac gagtaaaatt gtctttccca tctgtctctc ctttgataaa aacagtgcta
    27361 tactgcttta agtataacac tatttttatt gttttacgat gattttaatc tgctattgaa
    27421 agaaagtcct gattgctgat tgagttgtta aactaatctt agtcagccca ctatccatca
    27481 tttatttcct tagctgatta gttttcttct tttttgtttt tcaaacctag accaccgagt
    27541 aaacctgcaa gcgcaagccc aaggaaacca atacttgcca ttgatgtttg agtctcacca
    27601 gtatttggta gcatagcttt atcctctgac atcatcgtat cagacatctt gttagcagaa
    27661 gcagccatgt tttcacctgc catcgtgttg gtagaacttg tcatggtgtc agcaggcatg
    27721 ctatctgtag tacctgtagc atgattgtga ttcatcggag tcacgccaga accagagtta
    27781 gaaggtgata atgaaccatt tgctgtgtct gatgtttctt taacatttat cttaatagtg
    27841 acttttttag ttgctacgat gttgtccaag tctggtttac cgtctttgtt accatagaca
    27901 ttgactgtag cgctgtaagt ttgagtacca tttgctcgga actggtcaat gagcgcttgt
    27961 ttttctttgc cagctacatt tccgtccaag gctacttgat agaagtattg acctttggtc
    28021 ttcacgtttt cacctagtgg agatagggct gggtttttag cgtcgccgtt atctgaccat
    28081 ggtgccttgt cagatgcctt gagcaagaga cgagtcaaca taccatcacc tgcgaagagt
    28141 tcgtatggaa tcacatggtt gacacctgct gtgaatggac cttcaccctt ggccttttct
    28201 aggtaggctg ctggaacatc gatactgtct ttaacgttat ctttaacagc tttttcgaca
    28261 gtttctttag caactaatcc attgatgttg atgtctacat ttttagtagc aactagatta
    28321 gtcaagtcag ctttaccgtc tttatttccg taaactttaa cagtagattt ataagtttga
    28381 gtaccattag cgcgaagttg atcaattaaa gcttgacctt gtttaccaac agtattgcca
    28441 tttaagtcta cttcatagaa gtatttccct tttgttaatc cttcaagagg aggtaaagca
    28501 ggatttttag cagtaccatt gtcagaccaa ggagcattat ccgaagcttt tagtaataga
    28561 cgagttaaca taccatcacc agcgaatagt tcataaggaa ttacttggtt tacaccagca
    28621 gtgaaaggac ctttcccttc agctttttca agataagcag caggaacatc gatgctgtct
    28681 tttgtatttt ctttcactcc accttttact tgttcaggag tagtttctgc tggacttgct
    28741 tgtgtcccat ccacatcagc tgtatctaca gtttctttta aggcatctgt tagctctgga
    28801 ttttcttgag cttcttcatc tttttcaaaa agtgtatcca gactagaaga gtttgtttcc
    28861 tcgctcactt cagttctttc tgtaggtgtc ggaagtgaat tcgtatttcc agtaacgccc
    28921 tcaggtttcg cttgagtgag aacggtgtct cctggttttt ctgataagtc accctctttc
    28981 gttcccgatg tctcttcagg taagacttga cctggggtcg gattgaccac atcggcacgt
    29041 acagaacttg gctgaccaac taggacaaag aagccactag ccacaacaac tgaggcaaca
    29101 ccgacactga gacggcgaat agaccaacga gtatatcttt gatttggatt gaatttcata
    29161 ggattctcct tagagtcttt ctttatttga tgactctagt ataatctcct gaaattaaaa
    29221 aggactaaga aaaagttaaa atttttctta aatccctctt ataaaacact tatattaatt
    29281 aaatccataa acagatttgg tgatttgata gacgacattg gacagtttgc gatctggcaa
    29341 gacagaatgt ttggtcaaac ggctcaacat ggtcttacga atagcctgaa agacttctgg
    29401 atttttctgc tgaatatagg tccacaattg gcgttttttt gccagatgct ccgctgttcc
    29461 agatcggttg agcagggtac tggaaatcac cgtcgtgatt tcaatatgat tcagcagata
    29521 ttctcgcatt ttgggatgac tcacttggga caaataaagt tggtctacca agagtcgatt
    29581 gaccttgagt tgctggtcaa tgcacttaat catcacttgc tcattgacag actggtcctc
    29641 acgcccaatc aaataacgat agaaatcgac aggcagatag tacatggtct tgacctgctg
    29701 aaggggcgta aagacaaaga gattatcgac ataaaaagta tgttcaggca attagaactg
    29761 gctagcacgc aacaaatctg tccgataaat cagcgagtgc atcatgatat actggccttt
    29821 ggagaaattt ccgacctggt cccagccaaa aatctgccga acaggcaaga ctgaatcgta
    29881 actcatactc ttcttacaag actgaccttc cttttcatag acaaaattgg tcacaaagac
    29941 atccacctct tgacccttgc tctcaaattc ctgcaaggtt tcaagaattt tcaagtaggc
    30001 acgaggatcc acccagtcat cactgtcaac tactttaaaa tagcgcccag aagcctctgc
    30061 caagccacga ttgaccgcac cgccatggcc tttattttcc tgatagatgg ctctaacgat
    30121 attaggatac ttgctagcta aacactcagc gatttcctga gtctgatcct gagacccgtc
    30181 attgataatc aaaatcccaa cttgctcacc accaatcact agcgactcca cacagtaatg
    30241 aagataggct gctgcattat agctagaaat ggcgatagac aataacttca taatctgctc
    30301 ctttagggga ctgatttttt cttatactct tcgaaaatct cttcaaaccg cgtcaacgtc
    30361 gccttgccgt atagatgtta ctgacttcgt cagttctatc tgcaacctca aaacagtgtt
    30421 ttgagctgac ttcgtcagtt ctatccacaa cctcaaaaca gtgttttgag cagcccgcag
    30481 ctagtttcct agtttgatct ttgattttca ttgagtatta ctctctcttg tcacttcctt
    30541 ctattttacc ataaagtcca gcctttgaag aacttttact agaagacaag gggcttctgt
    30601 ctctatttgc catcttaagc atcaaaaaag aggggtcatc cctctttacg aattcaatgc
    30661 tactagggta tccaaatact ggttgttgat gactgccaaa atataggtat ctgctttcaa
    30721 gaggtcatct ggtccaaatt caacatccaa tggggaattt tcctgctctc ggaaacccaa
    30781 aatattcaga ttgtatttgc cacggaggtc taatttactc agactttgac ctgcccaaga
    30841 ctgaggaatt ttcatctcca cgatagacac atttttatcc aactgaaaga catcaacact
    30901 attatggaaa agaatggtct gtgctagaga ctgccccatt tcatactctg gcgagataac
    30961 cgagtcagct ccaatctttt ctagcacttt cttagcggtc tgacttttga ccttagcaat
    31021 aacagtcggt acccccaaac tcttacagtg cataaccgca agcacactcg actccagatt
    31081 ttcacctgtc gcgactacaa cggtatcgca ggtatcaatc cctgctgatc tcaataattc
    31141 ttcatctgtg atgtcaccaa tcactccacg cgccaaaact ggctcaaact gattgatgcg
    31201 ctctgcgtgg tcatcaatag cgataatatt catatcctgc ttggctaggg cagctaggac
    31261 actgctccca aaaattccca agcccaaaat tccaatcgta cgatctgaca tgctctttcc
    31321 tttcttaacc aatactaata tctgctttca tatagtgaat catatctttc ttttctggat
    31381 ggtaatctgc caagctaaca aacaaggtca agggaccaat tcgtcccata aacataagtg
    31441 gcatgataac actgagagcc aatttcccaa ggtcaggagt cagatttgcc gttacaccaa
    31501 ctgtactaag agctgaaatg gtttcaaata tgaggtggat aaagggagga ttgcctttgg
    31561 ctgttatccc tagcagaatc aatcctatca agaagctcat caaaaagata ataaagacac
    31621 taaaggattt ttgaaccgtt cgcggcgcaa tcgttcgtct cgcaacattg gcatgaggca
    31681 agcctagaag ctcacttcgt gcaaagacca agaggacaaa aaatgtcgta atcttgagtc
    31741 ccccagctgt tcctccaggt gccccaccta gaaacatctg taagatataa atcaaaagag
    31801 tcacaggatg agcctgagta taatctatcg tagaaaagcc agctgttcgc atcgtcactg
    31861 tttgaaaaaa gctaactaaa accttatcgg caacagggag attgccaatc gttccagcat
    31921 tgttccactc aagaaagaga gtagttgccg ttccaaataa caacaaacct atagtcaata
    31981 ataatacaag cttcgtatga aagtgcagac gtcctttttt ctttcttcct acatgaccag
    32041 ccaaatcaaa ccagaccata aaaccaaggc cgcctgtaat aatcaagcct gcaatcacca
    32101 gattgaccag taaatcggtc tgaaaagcaa ataaactggt gctccctaaa ttatcaaaac
    32161 cggcattaca gaaggctgag atcgctagaa aaatggaact aaaaagacca cgtccccagc
    32221 caagttgagg aataaggcga aaactaagca aaatagctcc caagctctca accaaaaagg
    32281 tcgtgagaaa aatagaatag acaaactttc tcaaagatcg agtttctcca taactaaaac
    32341 tatcctgaat agttgcacgg ctacgaagac taagcttttg cttgctctgg atatagaaaa
    32401 ccccaataaa ggtcatgagc cctagaccac cgatctgaat caagagcaaa cagattattt
    32461 ggccccatat attataggtg tgagctactg gaagggttga gagacctgtc acacagactg
    32521 cagagacagc agtgaaaaga tgatcaaaat aagtcgctcg tgagctttca acttggacaa
    32581 agggcaagct caaaagaaga gagcctagta aaatgaccaa ggcaaaactc aaaaaaatac
    32641 gacgggctgg cgataaacga gataagccta gctctatttt ttccttagat aatttgaata
    32701 acatgtttcc attgtaccat aaaaagctcg aaatgactta gataggataa ggcaaaattc
    32761 actaacacca agcctaaaaa atgccacctt tttattgata gcttaggcaa gaacaatact
    32821 tgatagatgt gactataggt caaaaaagca agacaggagc ttttaacgcc agctactcta
    32881 tatgcttttc aggagaaaat ttctcttttt gattataggc aatgagaacc aaggcatcgc
    32941 ttagaaagct agtcacagg
//
LOCUS       DAVKWZ010000021        30866 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000021 DAVKWZ010000000
VERSION     DAVKWZ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30866
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.021"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(1..319)
                     /locus_tag="VW854_001002"
                     /pseudo
     CDS             complement(1..319)
                     /locus_tag="VW854_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239016.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C family protein"
     gene            complement(338..634)
                     /locus_tag="VW854_001003"
     CDS             complement(338..634)
                     /locus_tag="VW854_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262252.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYELTEYNKMK"
     gene            complement(618..2777)
                     /gene="pfbA"
                     /locus_tag="VW854_001004"
     CDS             complement(618..2777)
                     /gene="pfbA"
                     /locus_tag="VW854_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEV0262253.1"
                     /translation="MLKIVKKLEVLMKYFVPNEVFSIRKLKVGTCSVLLAISILGSQG
                     ILSDEVVTSSSPMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNS
                     ISSNGTIRSNSQLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAV
                     GDGIHDDRQAIQDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILN
                     GINIKNHPSIVFMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLI
                     NSSGAFAIGNSNNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQ
                     IISKESIQIEPLTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTL
                     STQNPSNIKILNNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVN
                     AYSYKNTKDLLDLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVIN
                     NNSKETEQPNIELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSGKYI
                     SFIKSNANGKEPVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQSNSNNVI
                     DSNQKNGEFNSSKDNRQMNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVK
                     QKLPKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            complement(3907..5133)
                     /locus_tag="VW854_001005"
     CDS             complement(3907..5133)
                     /locus_tag="VW854_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEV0262254.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(5176..5868)
                     /locus_tag="VW854_001006"
     CDS             complement(5176..5868)
                     /locus_tag="VW854_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV0262255.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            complement(6041..7792)
                     /locus_tag="VW854_001007"
     CDS             complement(6041..7792)
                     /locus_tag="VW854_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0262256.1"
                     /translation="MQNKQEQWTVLKRLMSYLKPYGLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLAYANRSVALDALFLRPAMSLLKLLGYAVLMTYFGYRGLSIG
                     ITAGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALIDERTYEPLQENGQA
                     KVKEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLTLGG
                     TYHKMYSLQAGAMAYTL"
     gene            complement(7782..9527)
                     /locus_tag="VW854_001008"
     CDS             complement(7782..9527)
                     /locus_tag="VW854_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV0262257.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLFLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADLILVLQNGQIIERGRHEDLLALDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(9733..10029)
                     /locus_tag="VW854_001009"
     CDS             complement(9733..10029)
                     /locus_tag="VW854_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEV0262258.1"
                     /translation="MSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKY
                     PVVTDGVIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYE"
     gene            complement(10048..10185)
                     /locus_tag="VW854_001010"
     CDS             complement(10048..10185)
                     /locus_tag="VW854_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262259.1"
                     /translation="MTLNYIEILIKLVLTLKWLNNNVHFVKRLIDGKPTLLIKNGNIA
                     P"
     gene            complement(10182..10637)
                     /locus_tag="VW854_001011"
                     /pseudo
     CDS             complement(10182..10637)
                     /locus_tag="VW854_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            10771..11598
                     /gene="xth"
                     /locus_tag="VW854_001012"
     CDS             10771..11598
                     /gene="xth"
                     /locus_tag="VW854_001012"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEV0262260.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDAKYAEYLAKLDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            11610..11753
                     /locus_tag="VW854_001013"
     CDS             11610..11753
                     /locus_tag="VW854_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEV0262261.1"
                     /translation="MDYNAVIPELLVSNIEQSRSFYCGLLGFRIEYQRPEENFLFLLK
                     SVN"
     gene            11864..11992
                     /locus_tag="VW854_001014"
     CDS             11864..11992
                     /locus_tag="VW854_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEV0262262.1"
                     /translation="MKANYPIYRPLTKRKFRVSDPYIYPHEFAVLDPNGYFLRFSE"
     regulatory      12375..12470
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            12870..13451
                     /locus_tag="VW854_001015"
     CDS             12870..13451
                     /locus_tag="VW854_001015"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEV0262263.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAAAGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            13451..14713
                     /locus_tag="VW854_001016"
     CDS             13451..14713
                     /locus_tag="VW854_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEV0262264.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LSAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(14764..15225)
                     /locus_tag="VW854_001017"
     CDS             complement(14764..15225)
                     /locus_tag="VW854_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpnD"
                     /protein_id="HEV0262265.1"
                     /translation="MKTKQLVASEEVYDFLKVIWPDYETESRYDNLSLIVCTLSDPDC
                     VRWLSENMKFGDEKQLALMKEKYGWEVGDKLPEWLHSSYHRLLLIGELLESNLKLKKY
                     TVEITETLSRLVSIEAENPDEAERLVREKYKSCEIVLDADDFQDYDTSIYE"
     gene            complement(15222..15986)
                     /locus_tag="VW854_001018"
     CDS             complement(15222..15986)
                     /locus_tag="VW854_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-2 restriction enzyme DpnI"
                     /protein_id="HEV0262266.1"
                     /translation="MELHFNLELVETYKSNSQKARILTEDWVYRQSYCPNCGNNPLNH
                     FENNRPVADFYCNHCSEEFELKSKKGNFSSTINDGAYATMMKRVQADNNPNFFFLTYT
                     KNFEVNNFLVLPKQFVTPKSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVQDG
                     QVRDPEKVTKEFKQGLFLRKSSLSSRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKN
                     IFVKNNHIKEKIRQQLQILRDKEIIEFKGRGKYRKL"
     gene            complement(16167..16571)
                     /locus_tag="VW854_001019"
     CDS             complement(16167..16571)
                     /locus_tag="VW854_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV0262267.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVSI"
     gene            complement(16641..18122)
                     /locus_tag="VW854_001020"
     CDS             complement(16641..18122)
                     /locus_tag="VW854_001020"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEV0262268.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWEAYAYSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAAAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARTNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFVRVLEDAGVYKQTEQGQTAFMRFVEQVGILLD"
     gene            complement(18141..19319)
                     /locus_tag="VW854_001021"
     CDS             complement(18141..19319)
                     /locus_tag="VW854_001021"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEV0262269.1"
                     /translation="MTQHLTAETLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKREDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            19483..20490
                     /gene="galR"
                     /locus_tag="VW854_001022"
     CDS             19483..20490
                     /gene="galR"
                     /locus_tag="VW854_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEV0262270.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIEDKRLENFRNYSQAKGIYHDELIFQGN
                     FTAQSGYDLMKEAIQNLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTKLTLRESTLNQ
                     E"
     gene            complement(20537..21574)
                     /locus_tag="VW854_001023"
     CDS             complement(20537..21574)
                     /locus_tag="VW854_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV0262271.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRSCVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(21575..21928)
                     /locus_tag="VW854_001024"
     CDS             complement(21575..21928)
                     /locus_tag="VW854_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV0262272.1"
                     /translation="MNIKSASDLLGISADTIRYYERIGLVPPITRTATGIRDFQVQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(22172..23062)
                     /locus_tag="VW854_001025"
     CDS             complement(22172..23062)
                     /locus_tag="VW854_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEV0262273.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVIAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALSRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            23195..23731
                     /locus_tag="VW854_001026"
     CDS             23195..23731
                     /locus_tag="VW854_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV0262274.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTENYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNLPTSYLQHLVMSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            complement(23898..24704)
                     /locus_tag="VW854_001027"
     CDS             complement(23898..24704)
                     /locus_tag="VW854_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEV0262275.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(25049..26569)
                     /locus_tag="VW854_001028"
     CDS             complement(25049..26569)
                     /locus_tag="VW854_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEV0262276.1"
                     /translation="MTNLIATFQDRFGDWLTALSQHLQLSLLTLLLAILLAIPLAVYL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALMAL
                     LLGLSYSPALLAQKEKENLIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            complement(26562..27290)
                     /locus_tag="VW854_001029"
     CDS             complement(26562..27290)
                     /locus_tag="VW854_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0262277.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVEENIALIPEM
                     KGWTKEEIAQKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKILLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVS
                     NPETILKAPATDFVADLFGGSIHD"
     gene            complement(27305..28069)
                     /locus_tag="VW854_001030"
     CDS             complement(27305..28069)
                     /locus_tag="VW854_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV0262278.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHDIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            complement(28073..28516)
                     /locus_tag="VW854_001031"
     CDS             complement(28073..28516)
                     /locus_tag="VW854_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV0262279.1"
                     /translation="MKDSHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKSQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            complement(29198..29494)
                     /locus_tag="VW854_001032"
     CDS             complement(29198..29494)
                     /locus_tag="VW854_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV0262280.1"
                     /translation="MVEPNLESLIKDLYNHARHDLSEDLVAALLETAKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFIKREEAKYRGTATSALMYEELFKIL"
     gene            complement(29516..30580)
                     /gene="pepA"
                     /locus_tag="VW854_001033"
     CDS             complement(29516..30580)
                     /gene="pepA"
                     /locus_tag="VW854_001033"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEV0262281.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKDEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPEMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
BASE COUNT         9116 a         6948 c         5550 g         9252 t
ORIGIN      
        1 ctatccagta cccactcggt tgattagata attttctgat tttttgtaaa ataatttttt
       61 tatttaaaac actatttgta ccaattggat cttttatctc atttttccct ttttcaaata
      121 agttatctac tctatgatcc gttatttcca tttcgtttac taacatgacg cagtcaccta
      181 gcatcatata ctccaacttt ttttctgaaa gtttagcaaa tataaaagca aatgttggta
      241 agcgataata tagtatattg aaactagaat agtacacctc tacttctaaa acattgttag
      301 aaatcgattt gactgtcctg attgatttgt cctattatta tttcatttta ttatactctg
      361 ttaattcata tgagtttaaa ccgatttcat ctttaacctc gagtaaagca gtttcaaata
      421 tttgtttaag agtttttgat tctttacaat taaccgacaa actttctgat aaaatatgta
      481 caacttctga gactgaataa cctatctcct ctttagaatt atataaatct gtagctccac
      541 caataatcca aaaatactga ttttgtgaac ctacaatatc ctcattttct acggaacttc
      601 cttgtatcga acaaatttta tttatcttta ccataatact tcaacccttt tagtgtcaaa
      661 agtaaaccaa ttcctgtcac tgttaagaat agttccataa tcttgttcga accagtcttt
      721 ggtaattttt gttttacatc tactatttct ttagatttat taatatgatt ttcagtttct
      781 ctgccatctc caactatttt atagtttact tcttctgtct tattatcttg tttattgtcg
      841 atcttgtcat tcatttgtct attatcttta cttgagttaa actctccgtt cttctggtta
      901 ctatcaatta cattatttga attagattgt ttttcctctt tgtttttttc tttttcgttt
      961 ttatcactta aattatttgt tacaattttg taaagcccat tctccgttac aatattgaaa
     1021 ttaccatcgc tatcacgtat aacaggttct ttcccatttg cattagattt gatgaatgat
     1081 atatacttac cggataaatt ataaaattgg ttatttaaaa cggttatttt accctttgaa
     1141 tcctcaataa caattccttc tttaccaccg aatatactat tctctgacac tactaaatta
     1201 tcactaactc gtaataattc aatatttggt tgttctgttt ccttagaatt attattaatt
     1261 acattttttg ttacagtaat atctgatact tttcctaaat attctgcact atcttttgca
     1321 actcgtatcg cttttgtttt aggatcggca atattaaata tattttcggc gataaccacc
     1381 tgtttattta aatctaatag gtctttagtg tttttatagc tataagcatt tactaaagct
     1441 gctccgcttt ctcgataatg tacactctct cctttaactt tcttatcaaa gcgatttcct
     1501 ttgattaata catcagtgaa tcctgtaaaa cgtacacctg catacatcat gttatcaaaa
     1561 tgattattta aaattttaat attagagggg ttctgtgtcg acaatgtttg atagtgtgtg
     1621 ccaattgctg ttactaattc cccagattta tcacttttgc caaaatagga attttgaata
     1681 gtcacatttt cagatttttt cccatcatca ttcaaggcat aaggaaaacc ttttctagtt
     1741 aatggttcaa tctgaatgct ctccttactt atgatttgcc catccttcat cgttttgggt
     1801 aaggcttgcc caagaaaacg agaattatca actaatacat ttttcgaacc tgcaatttga
     1861 atagcatgcc cttgataact atccttgaat gttacatttt ttatagttac gttatttgaa
     1921 ttcccaatag caaatgcacc tgaagaattt ataagtggta gattttttgc tttagttcct
     1981 tcttcattca aagcaccgtt catatcaatc gtaccaccag aataactaat atcttctgtt
     2041 gggccccatt ctacttgcgc accatcatcc gtaaataaac ctgtcataaa aacaatggaa
     2101 gggtgattct taatatttat accatttaga attgtagctt tctcattcaa ttctaagtgt
     2161 gtatgacttt ttaaaaaaac aatttctttt actaaataag ttccttcagg aaaatataca
     2221 tttcctccac ctagcccttg agctgcagca tctattgcat cttgaattgc ttgtcgatca
     2281 tcatgaatcc catcacctac tgcaccataa tcttttacac ttaaagcagt atcttcaaat
     2341 tcttttttga taattctgtc acttataaca tcttccttat aactcttatt ttcattagta
     2401 gatgttactg tagattcaac tgttctgttg tctaattgtg aattggaacg aatagtacca
     2461 ttagagctga tactattaac acttaacgaa ttatctaaac cattagtggt tgaattagag
     2521 gcaatcattt cagcagaacg attctgatta acagttggtg aattatctaa atcattagta
     2581 attgcattag aagactcttt tgtagccatc ggtgaagaac tagtaacaac ttcatccgat
     2641 aaaatacctt ggcttcccaa aattgaaatt gccaatagta ccgagcaagt ccccaccttt
     2701 aatttacgaa tactgaatac ctcattagga acaaaatatt tcatcagaac ctccaatttt
     2761 tttactattt ttaacaattg attattactt ttcttaaaat agatagaatg acaccgctgt
     2821 cattatcttt atcatatatt ttccaaattg aattgtcaag gaataacaca agaaaatatt
     2881 aaaatttata ctcttcggaa atctcttcaa actaggtcag tgccgccttg ccctaagtct
     2941 aggttactga cttggtcagt tctatagtag actgaaacta taatagtaca cctctacttc
     3001 taaaacattg ttagaaatcg atttaactgt cccgatcaat ttgtcctatt cttatttcat
     3061 tttactatat ctacaacctc aaagcagggt tttgagctat ctatggccaa cttcctagtt
     3121 tgctctttag ccttccattc aaaagtgatg ctatctttct tagcttgaat ataaaatatc
     3181 ccatttacta tatttaaatt tattgatatg ttgtaaatta taaatgaaag aacaaatacc
     3241 ttgtcataca agcattcgcc agggatattt acctgaatat cgctcatatg gcggattgaa
     3301 actaaaacac tacaatatat ttcttaaata gaccttctat aaaagctaga tttaacctcg
     3361 ataaaaatag atgcagtgct tctaaaatac tggaataggg catataggta atcgtagaca
     3421 agtgttttat agattgtctc gcaggagatc taatgatgta tagcaagcat cactaaacat
     3481 atgggatatt cgtattatta taatatcaac atgctataaa ttctatcatg caaaagaatg
     3541 actagaacat gaaagacagg cacaaaactt tgaacaatat tctgaaaaca aaaaaagacg
     3601 cgagtttcca caatctcact catggaaact cacgtctttc aaaaaatagg gtgaattgaa
     3661 aactagaaca agacactaga ttttctaaat gatgtctagt aaggattcta aatgctacac
     3721 caaattgctc acatcttatt tcaaaccacg ggactcccac aagaaaggaa aatcctataa
     3781 aaattccctc tctgaggaga aagaggcctc aactacaggt gtaaactatt atcaattgaa
     3841 aacaacagtt tttacactca attagacttt ttagggacag gatcactttc gcttgactgt
     3901 agtttgtcat ttttttgata aagttagcac tttttcagaa actgttttga aagtctcaat
     3961 gatatagtct acttcttcat cgcttaattt agtatgaaga gggagggtaa tttcattctc
     4021 aaagaaggca taggccttag gatagttcgt catatcaaat ccaagattct tataggctgt
     4081 caagagagga agcggtttgt agtgaacatt acttgcaatt cctgctttag ccaattcttg
     4141 gatgatgagg ttgcgttctt ctaggcttgc tccttctaca cgggtgatgt agaggtggcg
     4201 tgaagattcg acagtttcag tcttgtgtgc caaaggatgg atgcgagaac ctgcaaaacc
     4261 actatcatag cggtccacaa tgtccttacg gcgttgcaac aaacttggat agcggtccaa
     4321 ttgtaccaaa ccaagtgaag ccatgatatc ggtcatgttg cacttatagg ctggtgtaac
     4381 gatatcgtat tcccatgacc ccagttgcat cttggcaaga gcatccttag tttgcccgtg
     4441 aagggaaagg atttggaatt ccttgtacat ctcttcgtca tcaatcactg gattggcttt
     4501 ccaagtcgca cttccacctt ctgccgttgt aaagttctta acggcatgga atgagaagga
     4561 agtaaaatca gcgatagaac cagaaggttg tcctttataa gtagatccca aagcgtgggc
     4621 actatcagag acaatgacaa tacggttaaa ggccttttgc cacttgcttg aagcggtaaa
     4681 gaagtcacgt tttttctcca cgacttggaa caaacggtca taatcgcaaa caatccctgc
     4741 gagctctact gggataatca ccttagtttt ctcagtgata gcttgctcaa gcaggtcata
     4801 gtccatctca aacgtatctg cttggatatc caccatgaca ggggttgctc ccacgtgcgt
     4861 aatgacacta catgaagccg tataggtcat ggctggaacg atgacttcat caccaggtcc
     4921 cacttccaaa acgcgtaaaa tcaactccag agcggctgtc gcagagttga gacaaacagt
     4981 cttaggtgtc tgtgtgtaaa gagacaagcg gcgctccagt tcttttgttt taggacctgt
     5041 tgtgatccaa ccagaacgca gggtatccgc tacttcagca atttctgctt ctgtgatatc
     5101 aggcggtgaa aatggaatat tgtaatttgg cattgatttg ctccttttat ctgtactcat
     5161 tttcttatga ctactttatt ttagtacctc aaacacggtt tgaaacatga ttttgatgtc
     5221 cccaaagaaa ctaaactctc ggagataggc gaggttatag cgcatctttt caggaagaac
     5281 atgctccaca taggcctgat caactgacag acctttctcc gtcatttgac tgatgatggt
     5341 gtcctcatcc ttgtagttga tgctggctgg agaggtaatt cctgcttgca agagcaaggt
     5401 tgccatcatt tcagggctat actgctctgt ataacgtggc acttcaggtc gtgtaccgac
     5461 aaaggacatc tcacctttaa ggacattgac caactgaggc agttcgtcca aacggacacg
     5521 tcggatgaaa tttccaacct tggtaatacg gctatcgtta gcagaagtca ccagacttcc
     5581 ttttttatcc gcatccgtca ccatggtacg gaacttccaa atcttgaacc gacggttgta
     5641 ctgggtcaca cgctcttgct tgtaaatcac tggccctttg ctatccaact tgatccaaat
     5701 gctcaagatg agaaagatgg gagaagttag aaccagtaag accaaggcca aaacccagtc
     5761 caagcaacgc ttgaaaatca gcgaaccctt ccttttagag acaagctggt agtaagactc
     5821 aacctcgctt gatttcattt ccacaggcaa gtcttcccat ttcagcatgc tgttctcctt
     5881 tgtttttatt atactatttg tcaacatttt tcattatacc acaaaatgga aaaggcggtg
     5941 aaagaaatct gtaatttgaa ggatttcctt ctagtactca atgaaaatca aagtacagct
     6001 ttgagattgc agataaagct gacatggttt aaagagattt tcaaagagta taggccatgg
     6061 cccctgcctg caaactatac atcttgtgat aggttcctcc cagagtcaag agttcctcat
     6121 gggttccact ctcgataatg cgtcccttat ccaagacata gatgcagttg gcgtcttgaa
     6181 tagtagaaag gcggtgagcg atagcaatag ttgttcggcc ctgtctcatc ttcgccagag
     6241 aagcttgaac caaactttct gtttcagagt caatattggc tgtcgcttca tccaaaatca
     6301 ggattttagg ctggctggcg actgttctag caaaggcaag aagctggcgc tgcccagtag
     6361 agaagctcga accacgctcg gaaacagggg agtcgtaccc ctgaggaagt tcttgaataa
     6421 aggaatctgc atccacaaag gctgccgcag cctgaacctg ctcatcactg atttcttggt
     6481 acatggcgat attggactta atagttccat gatagaggaa gggttcctgt aaaaccaaac
     6541 cgatgttttt tctcagctct tcttgactga aatccctgat atccacatca tccaagagaa
     6601 ctctccctga ctggaattca taaaagcgca tgaggacatt gataatagac gatttccctg
     6661 aacctgtatg acctacaaag gcaatggttt cacccttatt aacagagaaa gaaatgtcat
     6721 ccagaatcgg atgtttaccg tcatacgaga aacacacatg ttcaaaacgg atattgcctt
     6781 ctttgacttt ggcttgccca ttttcttgaa ggggctcata ggtcctctca tcgattaagg
     6841 caaagacacg gcctgcagaa accatagacg tttgcagtgt tgaaaagttt tgcgtcacct
     6901 caatcaaagg gtcaaagagg cggttgatgt actggataaa agcatacatg gtcccggccg
     6961 ttatcccgat agaaagacca cgatagccaa aataggtcat caagacagca taacctagaa
     7021 gtttcaacaa actcatggca ggtctcaaaa agagggcatc caaggctaca gaacggttgg
     7081 cataggccaa gtgctcttgg ttgatttcat caaattctgc ctgcaggcgc ttctcttgat
     7141 taaaagcctg aataatcctg attccctcga tattctctgc cagcttacta ttgatatctg
     7201 acaagagact tctggttttc tcgatgattt tcactgactt ttttcgatag agattgacca
     7261 aaaggaaaat caaggggaga aagagcaaga ctaatgctgt caaacgaaaa tccagcacca
     7321 acatggtata aagggttgtc agaaagataa aaactgctga gataaagctg gataaaatcc
     7381 cagaaaacat atcactaatc gtctcggtat cgttggtcaa acgagaaacg atagaacctg
     7441 ctggcgtctt gtcaaagtaa gacatgccca gtttctccat attggcaaag gcatcccgac
     7501 gaatatccct aacaatactg taagacacgc gcgcaaagag aagattgccg acatactgaa
     7561 ctacagtttg taggatgtag agaccatagt agaccagcaa aacggtcacg gctagttggt
     7621 taagattgct gagatactgg tcgataaagt gggaagccac aaggggaatg acacttttaa
     7681 tgaccgtcgt cgctaggaga aaactgagtg ccaaaaaggt aaggagtcca taaggcttga
     7741 gataggacat caagcgcttc aatacagtcc attgttcttg tttattctgc atcttcttct
     7801 cccttcattt ccagctgctg agactggtag gtttgggcat accagccatc caaagctagc
     7861 aagtcttcgt gcctgcctcg ttcgataatt tgaccatttt gcaggactaa aatcaaatct
     7921 gcatggacaa ctgcactcag gcgatgggca gtgataatgg ttgtcttgtc ctttcgcgtc
     7981 tccttgaggt tgtcgataat cgcatactct gtcttggcat ctacggcgga taaggaatca
     8041 tccaaaatca agatatcagg gtctaaaatc atagcccgac tcatagccaa ccgttgcttt
     8101 tgaccaccag aaagactgac tcctttttca ccaatcagcg tatcaaatcc ttgaggcatg
     8161 tctacaatat cttggtaaac tcgggctagc ttagtagctt cctcgaccgc tgaaaggggc
     8221 aagttaggat tgccaaagcg gatattgtct aggattgaag tcgcaaaaag aaactggtcc
     8281 tgaggaacat agcccatgag actgcgaagg tctgtcagac gatagtcccg aatatcgtga
     8341 ccgtttagat aaatggcacc cttatccaca tcgtattcac gcaagaagag cttgattaag
     8401 gacgttttcc cagagcctgt ctgcccaacc aagcccagtg tttgcccttt tgccaaacta
     8461 aagtgaatat ccgtcagtgt ttcctcattt tcaaaagcaa agctgtcaat ggcatactcc
     8521 aaacgcccat tttcaatacc atccagagga aactcagggt cttgtacagg agattcctga
     8581 gacaaaagat tttcaatccg ctggtaagaa accttccctc gctgagtagt attaaagagg
     8641 aagccgatgg ccataagagg ccagaccagc atatccaaat agctgataaa ggtgactaga
     8701 ttaccaactg taatctgccc ttcctgaacc atcaaggagc caaccaaaag cgttaaaaca
     8761 taggacgaac caacaaacaa gagaaccata gggtcaaaga gactatcata tttcatggtt
     8821 tgcaggttct tttggaaggt taattcattg actgcctgaa aagacttcaa ctcgtctgcc
     8881 tgataaccga aagacttggt cactttgata cctgatacgg actcctgtac cttgttattg
     8941 agttcagaaa aagcagcttg ggattcgcca aaggccttat gagtctttct ccctaggcga
     9001 ctagtcgcat aggccatgaa aggtaggggg agaatggcaa caagagtcat ctgccatgag
     9061 atgctaaaga gcatggtcaa caaagtcacc agagccgtga tagaggcatc caccgcagac
     9121 atgacaccgc cacctgctaa acgagtcaag gcattgatat cattggttgc gtgtgccatc
     9181 agatcacccg tccgataggt ttgataaaag gctgacgaca tttttgtgaa atgcttaaac
     9241 aagcgagacc gcatgatctg tcccaagcaa taagaggtcc caaggatata catacgccac
     9301 acatagcgca aatagtacat accaaaggct gcaagtagca agtaaaatag gctaagaagg
     9361 aggtcctgct gggttaattg ccccgatgtg atggcatcaa taacccgccc cataaccata
     9421 ggaggaatga gattgaggac ggaaaccaag accagggcca caattccgac tagataacgg
     9481 cgtttttcta acttgaaaaa ccaccaaagt ttttgaataa tggacataaa atccctttct
     9541 gattgcaaat ggaaacctga ggccaatact caatgaaaat caaagagcaa actaggaaac
     9601 tagccgcagg ctgctcaaag cactgctttg aggttgtaga tagaactgac gaagtcagta
     9661 acctacatac ggcaaggcga cgttgacgcg gtttgaagag attttcgaag agtacaagac
     9721 cccaggtttt tcttattcat aagttacgac tgtaacagca cccttgtcat attcagcaat
     9781 aaagatattg gctacattgt catgcccttg tttactgagg ttatcaagca accactcttc
     9841 gctacgacca atcgattcca agacatctac ttgaatcaca ccgtcagtca caactggata
     9901 cttaggattt tcatctccca tttgcacaac gatgagttgc ccattttgct cttgcacagc
     9961 tcgtttgact tgcttcatct ggaaaatccc ttggctacga agtttgaggg ctacatccga
    10021 tgcagacaaa ccaactgaac gacaggttta tggggcaata ttcccatttt tgataaggag
    10081 agttggctta ccatcaatca aacgtttcac aaagtgaaca ttgttgttga gccatttgag
    10141 agtcaagacc agtttgatta aaatttcgat ataattaagt gtcatttatt cacatccacc
    10201 agttcaattg cgtctgtctt atacaattcg actttctcta acaggtactt gtctggctca
    10261 cttccgttta gggcacgata atagcgatct cccaccttga taagtgcgcc atctgtgttt
    10321 gtggaagtat taacatagac ttctgacttg tctactccca aatctttgga aacaacctcg
    10381 atgaaatgaa gtgaagtttg aaatttattg ttagaaactt gattgttttg ataagtcgtg
    10441 atgctgacca aaagcagagc taaataaggc cagagctgaa atcatgacca actcacgaaa
    10501 ttcggttccc tttttatcat ggtatgcctt aaaagcaaaa aaactgtcac agctaataga
    10561 acaatcccaa agccaaccat gataccattt tgctgaccga tttggctgag tacatagtca
    10621 taagagtaga atttcatgta ttttcactcc ctttcataaa atcattctta attataaacg
    10681 aaagagagta atttttcaaa ctatacgttg gggaaataga tctttttttg gtataataaa
    10741 atctataatc tgaatgaaaa aggtaacttt atgaaactta tctcatggaa tattgattcc
    10801 ctaaacgctg ccctaactag tgactcagct cgtgccaaat tgtcccaaga agtcctacaa
    10861 accttggtcg ctgaaaatgc tgatatcatt gctatccaag aaaccaagct ttctgccaaa
    10921 ggacctacaa agaaacacgt ggaaatttta gaagaactct tcccaggcta cgaaaacacg
    10981 tggcgttctt cccaagagcc tgcccgtaaa ggctatgctg gaaccatgtt cctttataag
    11041 aaagaactta cacctactat cagcttccca gaaatcggtg ccccttctac catggacttg
    11101 gaaggtcgta tcatcactct agaatttgat gcatttttcg taacccaagt ttacactcca
    11161 aacgctggtg acggtctcaa acgcttggaa gaacgccaag tctgggatgc caaatatgct
    11221 gagtatttgg ctaaactaga caaagaaaaa ccagtccttg cgaccggtga ctacaacgta
    11281 gcccacaatg aaatcgacct tgcaaatcct gctagcaacc gccgttcacc tggatttact
    11341 gacgaggaac gtgctggatt taccaacctt ttggcaactg gatttacaga caccttccgc
    11401 catgttcatg gcgatgttcc tgaacgctac acttggtggg cacaacgcag caaaacttct
    11461 aaaatcaaca atacaggctg gagaatcgac tactggctca caagtaaccg tatcgctgac
    11521 aaggtcacta aatctgatat gattgactca ggtgctcgcc aagaccatac accgattgtc
    11581 ttggaaattg acttgtaagg attttctata tggactacaa tgcggtcatt cccgagttgc
    11641 ttgtatcgaa tatcgaacag tcccggtcct tctactgtgg tttactgggt tttaggattg
    11701 aataccaacg tccagaagaa aactttctct ttctcttgaa gagtgtcaac taatgttaga
    11761 ggagggaaca aaggatcaac tagctgaact gacctatccc tttggtcgtg gagtcaatct
    11821 ctcctttggc ataaaggatg tttcaaaact ctattaaaga gtgatgaagg caaactatcc
    11881 tatttatcgt cccttgacca agagaaagtt tcgtgtcagt gatccctata tctatcctca
    11941 tgaatttgca gttttggatc caaatggcta ttttttaaga tttagcgagt agaataataa
    12001 aaattgggaa aagtctttaa agttagaaaa aggcatattg tcaggtgtct aaaaacttga
    12061 tagtatgcct tttatgatgg aaaaatcttc taatactatt cgaaaatctc ttcaagccac
    12121 atcaacgtcg ccttaccgta ggtatatgat actgactctg tcaattctat ccacaacctc
    12181 aaaacagtga tttgaggttg tggatagctt cctagtttgc tctttgattt tcatttagta
    12241 taaatgttag cttacaacgg agtttttgtt caccccctct tctgtttgtt cgttttttaa
    12301 accaaaacac taacaaaata gttgatttat aaagttattt agtgtaaaat aaaaacattg
    12361 gctcaagcct atttaaaatt gaatatcgtt ttacttgttt atgtcgtgaa ttggcacgac
    12421 gtttctacaa ggtgccggaa cacctaacaa taagtaagtc agcagtgaga tatggccgtt
    12481 tttgccatgt ctatttaggg gacttactta gagattaagt aggtcttttt tgaatatttt
    12541 taatatttcc cttaattagt gcggacggga ggtgaaatta tcctgtgaat aatttcagga
    12601 atccagggaa tttcatccct aatttttgag cccctagctc tttcatttct aggcttaggc
    12661 taaaatagtt ccccgaacta ttttactctc aaaaatcccg tcatagacaa taacaagatt
    12721 attgtctatg ataccactac aacgggaaat atttgataag actcgcttcg ctcgtaatag
    12781 ttttatcaaa tatatagtat tagcaaaagg gatcactata gaaacattta aacaacaagt
    12841 aaattagctt ttagacttta ggaggtctta tgaaattatt agaagagcgc atcctcaagg
    12901 atgggcatat cttgggtgat aacatcctca aggtagattc ctttttaacc caccaagttg
    12961 actttagctt gatgcgagag attggtaagg tttttgcgga aaaatttgct gctgccggca
    13021 ttaccaaggt cgtaaccatt gaagcgtcgg gtattgcccc agccgttttt acagctgaag
    13081 ccttaaacgt tcccatgatt ttcgccaaaa aagctaagaa catcaccatg aacgaaggca
    13141 tcttaactgc tcaagtctac tcctttacca agcaggtgac cagcaccgtt tctatcgctg
    13201 gaaaattcct ctcaccagag gacaaggttt tgattatcga cgatttcctt gctaatggcc
    13261 aagctgctaa aggcttgatt caaatcatcg aacaggccgg tgccacagtc caagctatcg
    13321 gtatcgtgat tgagaaatcc ttccaagatg gccgtgattt gcttgaaaaa gcaggctacc
    13381 ctgtcctatc acttgctcgt ttggatcgtt ttgaaaatgg tcaggtcgta tttaaggagg
    13441 cagatctcta atgcaaactc aagaaaaaca ctcgcaagca gccgttcttg gcttgcagca
    13501 cttactagcc atgtactcag gatctatcct ggttcccatc atgattgcga cagcccttgg
    13561 ctattcagct gagcagttga cctacctgat ttctacagat atcttcatgt gtggggtggc
    13621 aaccttcctc caactccaac tcaacaaata ctttgggatt ggactcccag tcgttcttgg
    13681 agttgcattc cagtcggtcg ctcccttgat tatgattggg caaagccatg gtagtggcgc
    13741 tatgtttggt gcccttatcg catctgggat ttacgtggtt cttgtttcag gcatcttctc
    13801 aaaagtggcc aatctcttcc cctctatcgt aacaggatct gttattacca cgattggttt
    13861 aaccttgatc cctgtcgcta ttggaaatat gggaaataac gttccagagc caactggtca
    13921 aagtctcttg ctttcagcta ttactgttct gattatcctc ttgatcaaca tctttaccaa
    13981 aggatttatc aagtctatct ctattttgat tggtctggtt gttggaactg ccattgctgc
    14041 tactatgggc ttggtggact tctctcctgt tgcggtagct ccacttgtcc atgtcccaac
    14101 tccactctac tttgggatgc caacctttga aatctcatct attgtcatga tgtgtatcat
    14161 cgcaacggtg tctatggttg agtcaactgg tgtttatcta gccttgtctg atatcacaaa
    14221 agatccaatc gacagcacgc gccttcgcaa cggttaccgc gcagaaggtt tggcggtact
    14281 tctcggagga atctttaaca ccttccctta caccggattt tcacaaaacg ttggtttggt
    14341 taaattgtca ggcatcaaaa aacgcctgcc aatctactac gcagctggtt tcctggttct
    14401 ccttggactg cttcctaagt ttggtgccct tgcccaaatc attccaagct ccgtcctcgg
    14461 cggtgccatg ctggtgatgt ttggttttgt atctattcaa gggatgcaaa tcctcgcccg
    14521 agttgacttt gctaacaatg aacacaactt ccttatcgca gctgtttcaa tcgctgcagg
    14581 tgtcggtctc aacaacagta atctctttgt cagcatgccg acagccttcc aaatgttctt
    14641 ctcaaacgga atcgtcgtag ccagcctact cgctattgtc ctcaatgccg tattaaatca
    14701 taaaaagaaa taagaaaaag aggtgcgagc ctcttttttg tgtgacaaaa taaaaacatc
    14761 tacctattca tatatgctag tgtcatagtc ctgaaaatca tctgcatcaa gaacaatttc
    14821 acaactctta tacttttctc ttacaagtcg ttcggcttca tctggatttt cagcctctat
    14881 acttactaaa cgtgataaag tttctgtaat ttctactgta tactttttca gttttagatt
    14941 gctttccaat aattcaccta ttaacaataa tctatgatag gagctatgta gccactctgg
    15001 caatttatct cctacttccc acccatattt ttccttcatc aaagctagtt gtttttcgtc
    15061 accaaatttc atattttcag ataaccatct cacacaatcg ggatctgata aggtacagac
    15121 gattaaactt aggttatcgt aacggctttc agtttcataa tcaggccaga tgacttttaa
    15181 gaaatcatac acctcttctg atgcaacaag ttgtttcgtt ttcataattt ccgatacttt
    15241 cctctacctt taaattctat tatttctttg tctcttaata tttgaagctg ttgcctaatc
    15301 ttttctttga tatgattgtt cttaacaaag atatttttta ggtcactttc aaaacgatac
    15361 atatcttcaa gggtaaattc tgaaccctct atcttatcta tacaatttag aatttctatt
    15421 gtccaacctc ttgatgacag agagctcttc cttaaaaata aaccttgctt aaattctttt
    15481 gtaacttttt ctggatctct aacttgtcca tcttgcacaa gaaatatcct tcctttagaa
    15541 ggtacttgtg ataaatcaat gttacaacca atccaacctg ctcgtctagc agttggtgca
    15601 agtggttttc tttgaataat cgatttcggt gtaacaaatt gcttcggaag gacaagaaag
    15661 ttatttacct caaaattttt tgtgtaagtt aaaaaaaaga aattaggatt attatctgcc
    15721 tgcacacgct tcatcatcgt tgcataagca ccatcattga ttgttgatga aaaatttcct
    15781 tttttgctct ttagttcaaa ctcctcacta caatgattac agtaaaaatc tgctacaggc
    15841 cgattatttt caaaatgatt taagggattg ttcccacaat tagggcaata agactgacga
    15901 taaacccaat cctccgttaa gatacgtgcc ttttggctat tacttttata ggtttctact
    15961 aattctaaat taaagtgtaa ttccatttaa aaattcctca ctaatatgag aactcactcg
    16021 atataagcaa ttaaaatcta ctataactag tataccacat atatgtacct attacatttg
    16081 tatttcatca ctctctttta tatttccttc taacatatct cataaattaa aaatgagtga
    16141 gataaattta tcccactcac ctttctttat atacttaccc gtctttcctc agacctttgg
    16201 cctgtgacaa acatggtaaa ggttgcctta cagacatttc tgccctcttg attggtgata
    16261 tccacatcca tcacacaggt tgtacgacct tgatggacac attctccttt aatggtcaat
    16321 acatcgtcga gttttcctgc cttaaggtag ttgatagagg attggagtgt caccccatca
    16381 agtcccagcg agataaccac caaaccactg atttggtcac atagcgtgaa tagatagcca
    16441 ccatgggcat tgccatagta gttgagcgat gagttcacta ctttggtcgt caccacaacg
    16501 tgaccatctc tcattttttc aatttcgtaa ttttcaaagg cagatatagc gtcaaaatga
    16561 aagtctttca tcgtttcctc ctgatatttc aaacgacact atgatactac tttttatagg
    16621 actgtgcaag gagaaagctc ctagtctagt aaaattccga cctgttccac aaagcgcata
    16681 aaggctgtct gcccttgttc tgtctgcttg tagactcctg catcctcaag tacacgcaca
    16741 aagatagcac ccacagagtc cttgacgatt gcaagggctt tttctttatc cgttaggtct
    16801 ggatgttggg atttgagttg gtctgcccac tcctgatgat aatcggcaac tgtaacagct
    16861 tctcctacaa gatagctagc gacttgctct acttcttctt tcagacgtgg tggcaagatt
    16921 gccaagccca tgacctcaat caagccgata ttttccttct tgatatgttg gacatccttg
    16981 tggggatgat agatgccatc aggatgctct gctgaagtct gattgtctcg caagaccaag
    17041 tccaactcaa actgtccatc acgcttacgc gctattggtg tgatggtgtg atgcggtgtc
    17101 ccgtctgtct ctgctaaaat ctgcacacta gaatctgaat actgacgcca ttcctggaaa
    17161 atcttatcag ctaagttaat caaatcctct ttggaatccg aagtcaagcg taggacagac
    17221 attggccact tgataattcc agccttgacc tgctcaaaac cagcaaatcg gaaggtcttt
    17281 tgcaagggag ccaattccat aggaaatacg tgacggcctc cctgataatg atcatgagtt
    17341 agaatagagc cccccacaat cggcaggtcg gcattagatc cagcaaaata tcctggaaac
    17401 tgctctacga tagccaacag acgttcaaaa ctctgacgac taatggccat gggacgatgc
    17461 tggccatcta aaaagataca atgctcatta aagtaagcat agggcgaata ctggaaaccc
    17521 cattcctgac caaccatttc aaaacggata atccggtggt tggtgcgagc tgggtggttg
    17581 acccgaccat ggtaaccctc attctctaaa caaagttgac actgaggata attactagct
    17641 tgcaccaact tggctgccgc aatctctttc ggatcctttt caggcttaga gagattgatg
    17701 gtaatttcaa gttctccata atcagatgga acacgataag cgatattttt agcaatggcc
    17761 ttgagtttga tgtagtcatt tttctgactg agttgataaa aatcctctat cgcttgctca
    17821 ggagagtagg cataggcttc ccagaagtca cgattgacct gactcggaca aggagtcacc
    17881 aagtccatca attcagtacc aaggatttca cgcgcagtct gactatcctc aatcgtctct
    17941 aatcgaacgg cttcctcaac tagctggtcc ttgaggtcaa tcaatttatc cagattggtc
    18001 tcaacttcca aaacaccttc tcccactcgt gccaaaacac gattggtcag gtagattcga
    18061 tccatttcct caaatgaact ttcagaaatg acatgtgtta caaatttatc tactaaggtc
    18121 actatagagc ctccttttga ctagtcaagg acgcgagtgc cacctgcaac ttcagcgata
    18181 tagaagcttg gagcgtatcc aactacttcc tcgtagtgtt tgcctacagc ttccttaaag
    18241 gcctcaacag tatctttttg aaccaaggca atggcacagc caccaaaacc agcccctgtc
    18301 atacgagcac cgagaactcc ttcttgtgcc caagctgtgt gaacaagggt atctaattcc
    18361 aaaccagtta cttcataatc atgctccaga gaaacgtgtg acgcattcat caaacgacca
    18421 aatgtttcca aatcacctgc ttgaagggct gcttgagctt taagggtacg ttggttttca
    18481 agcacagcat ggcgagcacg tttcaaacga ttttcatctt taatcaaata gctatattgg
    18541 tcaacggccc actcgtccaa ttcacccagg gtctgaatat ccaaggcaac ttgcaattct
    18601 tccactgctt tttcacactc agcacgacgt tcattgtatt tagagtccgc caattcacgg
    18661 cgtttgttgg tgttcatgat aacaacgaca ttgtccttca aatcaagtgg caccaaatca
    18721 tactctaagg tgttggtatc aaggtaaata gcacgttgat cagcccccat accaatagca
    18781 aactggtcca tgataccaga gttgactccg ataaagttgt tttctgtttg ttttccgatt
    18841 ttaaccaaat cgagacgctc taattttaaa tcaaagagat gctctgccac gactcctgtc
    18901 aagagctcca aggatgctga agaagacaaa ccagcaccat ttggtatatt cccataaaca
    18961 taaaaatcaa aacctttgtc aatcacgtgc ccagcttctt gcaagaaatg aaggacacct
    19021 tttggataat tggtccagtt gtgctctttt tcaaacttga ggtcagcgag aggcacttcg
    19081 ataatgccct tgtcctcaaa gttagctgag tagaaacgta aaacttggtc ttcacgcttg
    19141 cgagctgcac cataagttcc caaggaaata gcagctggaa aaacgtgccc accgttgtag
    19201 tctgtgtgtt caccaatcaa attgatacga cctggtgaaa agaaggtttg gtctgcttct
    19261 tgaccaaaaa cagcaagaaa gtctttgcga agagtttcag cagtaagatg ttgtgtcata
    19321 tgatttctcc tttgactgtc cgttaagcca ccttaacgtg taatcgtttt cttctattgt
    19381 atccaaggga tttcccaagg tcaatccttt ttactaaaat tttactaaaa tatcagtaaa
    19441 aagcagaaaa atatgatata ctaacctaat caaggaggat ttatggctac cttaaaagac
    19501 attgcacagc tagcctctgt ctctatcgcg accgtatccc gtgttctcaa ccgcgaccag
    19561 agcctatctg ttacagaaga aaccagacac cgtattttaa ccgttgctga agagctgggc
    19621 tacaccaagc acctcaagac aggcgagtcc cataaaccca agcaaaagat tgccattatt
    19681 caatgggtca gcgaacaagg ggagctagac gacctctact actaccagat tcgcctagga
    19741 atagaaaaaa gagcccaaga gttggactac gatatcttgc gctattttaa tgaccaccct
    19801 tttaccctaa gcgaggaagt gattgggatt ctctgcatcg gaaagtttag tcgagctcag
    19861 atttctgcct ttgaagaata ccaaaagcct cttgtatttc tagacagcga tacactttcc
    19921 ctgggacata cctgtattat cacggatttt tacactgcta tgaaacaggt tgtcgattat
    19981 ttcctcagcc aaggaatgga ccgtatcggg attctaacag gccttgaaga aacaactgac
    20041 caagaagaaa tcattgagga taagcggctg gaaaatttca gaaactacag tcaagcaaaa
    20101 ggaatctatc atgatgaact gatctttcaa ggaaacttta ctgcccagtc tggctatgac
    20161 ttgatgaagg aagccattca gaacttggga gaccaactcc caccagcatt tttcgcagcc
    20221 agcgatagtt tagccatcgg tgcccttcgt gcgctccaag aagctggaat cagcctacca
    20281 gaccgtgtca gcctcatttc ctttaacgac accagcctga ccaagcaggt ttatcctccc
    20341 ctctccagca tcacagttta tactgaagaa atggggcgag caggtatgga tattcttaac
    20401 aaggaagtcc tccacggtcg gaaaatccct agcctgacca tgctgggaac caaactgacg
    20461 ttaagagaaa gtactctaaa tcaagaatag gataacataa aaaacgaata ggagttttag
    20521 aactcctatt cgttttttat tcgattacaa tcatagactt aatggtctta cgttcatcca
    20581 tatctttgta ggcttggtcg atatcttcca gtttataact tgaagtaaag acgcgacctg
    20641 gattgatatc accatcaagg acggctttta gtaaaaattg cttatcgtat gttgtagcag
    20701 aagctgcccc acctgctaca gagatatttt gcataaatgt cgaaccaaga gcacgattat
    20761 tatagtgtgg gactcctaca aagcccatac gccctccatt atgaagaaca cctagcgcct
    20821 gttctatagc agcctccgta ccaacacatt caagtgctgc atctgctcct ccaccgagga
    20881 tttcacgcac ctttgtaatt ccttcttgac cacgttctgc aacaacagct gtcgcacctg
    20941 actccatagc catcttttga cggtcttcat gacggctcat aaggataatt tgtgatgctc
    21001 cacgcatctt agccgcgatg acagcacatt gaccaacagc cccatcacca ataacaacaa
    21061 ccttgtcccc tttttgaaca tttgcaacac gcgccgcatg atagcctgtc ggcatgacat
    21121 ctgcaagagt caaaagggac ttgagcatcc cttctgtata gtcagaaggt tgaccaggga
    21181 ttttaaccag cgcccagttt gcatagtgga agcgaatata ttctgcctga aaatcacccc
    21241 ccaaattatt gccaatatga ttgtcgcaag aaccgtcaaa tccagcaaga caggcatcac
    21301 actcaccaca tccatgtgta aaagggacaa tcacaaaatc acctggtttc accgtcgtaa
    21361 tggcttcccc agcttcttca acaatcccaa tcgcttcgtg tccactattt ttgtgtccag
    21421 ctttcgtttc tggattacgg tacctccata aatctgaacc acaaacgcac gaacgaacca
    21481 cacgaataat cacatcatcc gcttctatta tttgcggacg ttcaatgcta gcaagtccaa
    21541 cctgacctgc ctttgtatat actgctgatt tcatttaaaa ttttccttcc ttataaagtt
    21601 taattttgag atttaaacga tttaaagctg tctgtagctg agacaagcgc tcctctaatt
    21661 tttgcttttc ctcttctaaa ataccaagcc tctcctctct cgtttcatct cccttttggt
    21721 agagcgacat atagtcaact aaactatcta cagagacacc cgccgaacga aaacacttaa
    21781 taaattccag cgcttcgata tcctgaactt gaaaatcacg aatcccagta gcagtacgag
    21841 taatcggtgg cacaagacca atccgttcat aataccgaat cgtatccgct gaaattccca
    21901 acaaatcact ggcggattta atattcacac gctaacctcc tctcatttca ttcactgaat
    21961 tcacgctatt ctaactcact tatcttatta tataactttg agttgactcc aagtcaagca
    22021 ttttgtatct aatcgaaaaa tttttatcat taacaatcat atatctggag tttatacaat
    22081 gctcttctct taccaatttt tgaaatattt aaataataaa gaaattactc tattccaaat
    22141 aagatagtac ccaagtattt ttcacttttt tctaatgttg atgctcataa ctccgttcca
    22201 agtcacacac ctttcgctta tgggtttggt gagtttctag gtcagcatca atttcaatgg
    22261 taatattttg aaaaccacaa tcttttagga aaattcgaat agactcttta caagtttcca
    22321 tatgttccat ttcttttaga caaacatgga caatggcatt tttttccaaa gcatccatag
    22381 tccagagatt aagctgatta aggctggcca cattgtccaa tcgctccagg ccactcttta
    22441 cttgcttgat atcaagacct tctggcacag catccaagaa aatcttgagt gtagaccaaa
    22501 aacgtgaaag ggcttttgaa agaataaaga aagaaatgac aagggacaaa agaggatcta
    22561 ggatatacca gtccgtaaat cgaagaacaa tcgccatcag gataacagct acccacccta
    22621 gcgtatcttc cagaaaatgc agactcagaa tagactcatt ctttgtcttt cccttaccaa
    22681 ccaccagact cgctaacaga ttgatagtaa tcgcaataat tcctaaccag agaatcccct
    22741 catcattgac tggttgcgga tgcaaaatct tcgtgacatt ttccaaaatg actagaacag
    22801 agcccgttac gagaatcaca gctattacca aggctcctag caggctaaac cgcttatagc
    22861 ccaaggtgta ctgattgtct tcttcacgat tggagattgt ttctagaaaa gctgatattc
    22921 caattgcaat cgcatctccc aagtcatgca cagagtcagc aagaacagcg ctagaaccaa
    22981 atactccacc tgcaataaac tcaacaatgg cataagtcaa atttaagaaa aaagccaccc
    23041 aaacagcata ttttgccttc atatttctca ttcctttgtt ataatagatt tatgaacacc
    23101 ttgttcatta tcattatcca ctaaaactca aagaaaggat agaagcaaaa cgtcagcttt
    23161 attcagttct gaacaatttg ccttaagtgt ccatatgact aacattgacc gccgtatcag
    23221 caaaaccaaa aaagccatct atcaagcttt tatacaactt ttgaatgcta agggctacga
    23281 ggccactact gttcaggata tcattgatct cgcagatgtg ggacgatcca ccttttactg
    23341 tcactatgag agtaaggagc ttcttctgga ccaactctgc cgctacctct tccatcatct
    23401 ttttgaaaga gagcaagcca tttcaaccga gaattacctc gcccacctct ttctccattt
    23461 tcataaaaac caagaccata tcaccagtct gctattttcc aaaaatgact acttcctccg
    23521 tcaactccat aaagagctag aacaccatgt ctattccgtg ctagctgata atttaaaaga
    23581 agcccaccct aatctgccta cttcttacct ccaacacttg gtcatgtcca actttatcga
    23641 gacattgacc tggtggctca aaaaaggtca agatttcaca gaccaggaag ttgtccaatt
    23701 ttatctagac cttctcattc ctaaaaattg aatatagagt aaagcttcag ttgtcttatt
    23761 tctaggttac tgagtttttt atcttttcaa caacaaaaga ggacccgccg atcctctttt
    23821 tcatactata aatccttgat tatcaactat atctgtttta atcgaaatct caaaacagca
    23881 ctttcaaaca tcttttccta gttaagtaaa tcagtattct gcttagctgc cttgctccat
    23941 tgataccaac caactagact gttaatgaga taaattagat atttcccttg aatttgcagg
    24001 ctttctcccc accagagata gattgaaaaa acattggtag ccgcccagaa tatccactgt
    24061 tcacggtaaa cagctgtcat gaggatttgc cctaccccat tggttgcatc tgtgattgaa
    24121 tcacgatagg gacgattggc accaatagac tgataaatga agccaaaggc caaccaccaa
    24181 agcacactaa tggaaagata ctttgtccag cccttgccgt ccagtttacg cgcgacaaac
    24241 tcctgctttt ccttcttaaa ctgtgcctga taaatccaaa ctagaagtcc aattggctgc
    24301 atgactgtga agtaaagtgt cgtcagcacc tcaccataaa agcctttctg tagggccaaa
    24361 ataaggtaaa taacagagtt aatcaagcca aaaagataat tacttgctcg accttccgat
    24421 acaaagatta cacagataat ccctgtcaag ctacaaatca tcccaatcca gtcaacaata
    24481 cgatgttcgt aaaccaactc cagccagaga ggaaaacttc ctaaaaccag caaataaatc
    24541 cactgggcaa aactacgatg ggcaaagagg tcatcccaga tagccttcat agttcctgaa
    24601 aatcctaaat cagccatagc cgcaaccata cgacggtaac cacctgacat ttcacctagg
    24661 gttgttttga tattttcaat tttcttttgc aaataagtat gcatcatttc tccttttgtt
    24721 tttaaagagc cgtgtctgga tagactttcg gacgcaacgc tctattagat aatgaactgc
    24781 ctatacacaa gatttctaac cttagtcgac atgagctgaa acctcttatt tgttaagtag
    24841 ttcacaaaat attatacacc tattttatga atagtcaact gtctttacag taaaatttta
    24901 gaaaatcatg aaaattttct ctttctttcc attttaagtg acattcagtc attctcacat
    24961 caaaaaagcc cagacgaaat tgtctgagca ttcttttatc tagtcgttta aggaagttga
    25021 gttcagtatg tttaaagtct ctgtcccatc atttcttcaa caaaccttgt tcttggagaa
    25081 actccttggc tacttgcttt gctgacttgc cttcaacacc gacttggtag ttgagctggc
    25141 tcatctggct ttctgtaatc ttaccagcca atgtattaag aactctttcc aactctgggt
    25201 gtttcttgag aagagcttct ttcatgagtg gagccccttg ataaggtggg aagagttgct
    25261 tgtcatcttc caagacctgt aaatcataac gctccaattc cgcatcagtc gaataggcat
    25321 ccgtgatttg aatatcccct gactgaattg cctgataacg aagagctggc tgcatagtcg
    25381 ccacattgag attgagacca tacattgatt gcaagccctt atttccatct tcacggtcgt
    25441 taaactcaag cgtaaagcct gccttcagct gcccttccac ttttttcaaa tccgaaatgg
    25501 tcttcaagcc atattcttga gcaatctttt tcggaacagc tacagcatag gtattttgat
    25561 aagacatagg tttgagatag gctagatgat cctgcttggc aataccatca cgcgccactt
    25621 gataaacctg ctctggctca tgactcacct tgggtgatgg ttgaagcaaa ctttcagtca
    25681 ccgtaccggt aaattcagga taaatgtcaa tatcaccttt tttcagagct tcataaagga
    25741 aacttgtcgt cccaaaattc ggtttaacag tcgcagtcat gctggtattt tcttcaatca
    25801 gcaatttata catattagcc aaaatttctg gttctgggcc tattttccca gcaataatca
    25861 agttttcctt ctctttttga gccaaaaggg ctggactata agacagaccc agtaataaag
    25921 ccatcaaggc aaaaccagag aaaatcgtcc gcaatttcgc tttttccatt acttttagta
    25981 ggaagttaaa ggcaatagct agcactgcag aagaaagggc cccaatcaaa atcaaactgg
    26041 cattattacg gtcaattccc aacaggataa aggaacccag tccccctgcc ccaatcaagg
    26101 tcgccaaggt agccgtaccg ataatcaaga ccgctgccgt ccgaattcca gacatgataa
    26161 caggcatggc aagtggaatt tcaaacttct tgagacgctc ccatctggtc atcccaaagg
    26221 caatcccagc ctcttgaaga ctcggatcaa ttcccttcag cccagtaata gtattttgta
    26281 aaatcggaaa aatcgcataa atcactagag ctgtcaatgc cggtaaagtc ccaattccca
    26341 tcaagggaat aaagagtccc aacaaggcca gagacgggat ggtctggaaa atccctgcaa
    26401 tctgtaagac ccaatccgcc aacttctcat gatagcgaag ataaacagcc aagggaatcg
    26461 caagcaaaat agctagtaac aaggtcaaaa gtgacaactg caaatgttga gatagggctg
    26521 tcaaccaatc accaaaacga tcctgaaaag ttgcaattaa attagtcatg aatactacct
    26581 ccaaacaagt ctgcaacaaa gtctgttgca ggagctttta aaatagtctc aggattcgac
    26641 acctgacgaa tctctccatc ctgcaagaca gcaatacgat ctgccaactt taaggcttca
    26701 tccgtatcat gagtcacaaa aatcgttgtc atcccaaact ctttatgtaa ttcttttgtc
    26761 agaacctgca actgcttgcg agaaatagca tccaaggctg aaaagggttc atccatgagg
    26821 agaatcttgg gctgaccaat catagctcgg acaataccga cccgttgctg ttctccacca
    26881 gatagctcac ttggaagtcg atgcccatac tcagctactg gtaaaccaac cttagccaaa
    26941 agctcttctg ttttttgagc aatttcttcc ttagtccaac ccttcatttc aggaatgaga
    27001 gcaatatttt cctcaactgt tagattggga aagagagcaa tagcctgtaa aacataacca
    27061 gtagaaagac gaagttcacg ctcatcatag tctttgatgc gcttcccatc catataaata
    27121 tttccatcag ttggttccaa aagacggtta atcatcttga gcatggtcgt cttacctgac
    27181 ccagaaggcc ctactaaaac cataaattcc ccatcctcaa tctgtaagtt gacatctctc
    27241 aagacatcct tttctgtgta gcgcagtgct acatttttgt attcaatcat tctttgtcct
    27301 caatttaaaa cttccctcga ttagtcaagt cttctacctt aggcatgact tccttattat
    27361 cccaatgctc cacaattttc ccgttctcta aacggaagat atcgtactgg gcataagcaa
    27421 cgccatcaat ctgagtctga ccatagctaa ccacatagtt tccttgtcct aagagttgga
    27481 aaacaaagtc aaaagtgaca ctatattcag ccacataatt tttataagca gcacttcctt
    27541 gtccaatatc atgattatgc tgaatcaaat cgtctgccac ataatcactc cactgctcta
    27601 gctccccatt ttggaaaatt tctgtcaaga aacggcgaac cagcttttta ttttctgctt
    27661 tcttgtccaa atccttgatt tcaaaatctc caaaaatttg atctagttgg tcattttcag
    27721 gtgttcgata gtagtcaatg acatcccaat gctcaacaat acgaccattc tcatccgcac
    27781 ggaaagtatc cgtcgtcacc cattgagctt ctccaccatt cagatattga tgaacatgaa
    27841 caaagaccag attgccatcc tcaatggtgc ggacaatctt aatctgacgc tctggatgac
    27901 gctcaaagaa atctgcaaag aaggctgcaa atccttcttt cccgtcagga acacctgtcg
    27961 aatgttggat ataggtatcc cctacagact gggcttgagc ctcagcaact cgtccgtctt
    28021 gaatggcatg gatgtatagg ttgtgagcat ttttcacttg ttgtgacata ttctaaacct
    28081 catttccctt ctctttcaga ttcgccaaaa ttctttcttg aaaaccttca aattggtgaa
    28141 tttcttcctc tgaaaatcct ttgtaaaaga tagtatccaa tttctgactg acacgatgcc
    28201 ccacttcttt ctgggacttg cctaactccg ttaaaactaa atacttctta cgcttgtctt
    28261 ttccacacgg actaacaatt acaagctttt gttcctctag cttttttatc atagtcgtca
    28321 gcgtattatt cgcaagccca gtcgcaagcg cgatatctgt cgcagttgcg cagccagttt
    28381 cactattcca taaaaccgct aaaatcttgc cctgttcacc cctataaaga gcctcaggat
    28441 cttgactcag taacttttga aaaatccgcc cattcaacaa acgaatatga tgggctagca
    28501 aatgactatc tttcataaca cctccaattt atgtcgatat cgaaatgaat aaaataattg
    28561 taacactcat cgttctaact gtcaactatt tcgatttgga aataattttt gataattatc
    28621 cacaccacca tactccgaat caactaactt ttaacgagag tttctaaatt ccttcgtcct
    28681 ccagtctaca aaagccttcc atccgtacta tcctatattt tatgagggga cacatttttc
    28741 ctatcagacc atttatttta aagatagaag taaatcataa ttgcttccat ctgttctttt
    28801 atagtatatt gaagttgaac tagggcactg tatcttctaa aacattgata aaaagcgatt
    28861 tgaatttccc aatcaatttg ttcgtattta tagcattttg aaattggaat aggacaccat
    28921 gactgctaaa agatttctat aaattcattt gattttccta atcaatttgt tcatatctta
    28981 tttcatcccg ctataatttc accttgccct atctttttcg tagcaccctt caaacagcct
    29041 atcccctacc gtttgacgat tcctcacttc gctccacttc cattacagaa gtttcttcac
    29101 tactatggtc tcggctgact tctcatgatt ccttgttact actatttgaa cgctcacgag
    29161 ataggtctta caaaaaatgc tttgatccac aatggaatca aagtatttta aagagttcct
    29221 catacataag cgcagaagtc gcagttcctc tgtacttggc ttcttctctt ttgataaagc
    29281 gagccaagtt gagcaactca ggtgctggat gtttgggatt taggagcaat tcacgattga
    29341 ccaggcctga gagacgaact gcctgcaatt gctcatttgt agtaggcagt tttttagcag
    29401 tctctaggag agcagcaact aaatcttcac tcaaatcatg tcgagcatga ttgtaaagat
    29461 cttttataag gctttctagg tttggttcta ccatccctac cacctccctt atggtttaat
    29521 aatgtttaat caaatcaacc gttgaacgat ccaatttctt caccaaggct tgtaagaaag
    29581 cttgcgcttc taggaagtca tccattgcat agagggtttg gtgagaatgg atataacgag
    29641 cgcagacacc gatagttgtt gatgggacac caccattttt cagatgagct gcacctgcat
    29701 ctgttccgcc tttaccacag tagtattggt acttgatgcc agcttcttca gccgttgtca
    29761 aaaggaaatc cttcatctct gggagaagca agtgacctgg atcatagaaa cgaatcaagg
    29821 ttccatctcc aatcttgcct tgaccaccgt agacatcacc tgctggtgag caatcaactg
    29881 cgaggaagac ttctgggtca aacttggttg tagaggtatg agcgccacgc agaccaactt
    29941 cttcttggac gttagaaccc agatagagtt cattgccgag tttttgacct gataaagctt
    30001 cagctagctc gcttaccatg aggacaccgt agcggttatc ccaagctttt gagatgatat
    30061 ttttttcatt ggctgtcaaa attgcagaac tatctggtac gatggtatca ccaggacgga
    30121 tgccaaaact ttctgcctca tccttgtccg caaaaccacc atcaaaaacg atatcggcaa
    30181 tggctggcat ggttggtccc ccctttccac gagtcaaatg cggaggaaca gaacctgaaa
    30241 tcacaggaat ttcatgacca tcacgagtca agagtttgaa acgttggctg ctaaccacca
    30301 tggggttcca gccaccgatt tctacgacac ggaaggtacc atctggcttg atttcgctga
    30361 ccataaaacc aacttcgtcc atatgagaag cgaccaagac gcgcggtgca tccacagctt
    30421 ctgaatgttt gataccaaaa ataccaccca agccatctgt caccacttca tccacatgcg
    30481 gtgtcaactt ttcacgaaga taagcacgga caggcgcttc atgacctgag actgcagcaa
    30541 gttctgttac ttctttaatt tttgaaaata atgttgtcat ttcagttcct tctttctttc
    30601 atccatttta ccacttttta taggagaagg atagtgggaa ggtggatttc taagttagta
    30661 tcttagtcct gctctatctt agaaaaggat agtattctct tgcatgtagt gcaaaatcta
    30721 gtaaacattc caaaattaac tcgaatattt atttccaaac aaaaaaacaa tacaccatca
    30781 aagttgttta ggtttttcat gaaatttaca gaaaatagtt gacttccctt tcttctttct
    30841 ttaaatatat agttggttga gtttgg
//
LOCUS       DAVKWZ010000022        30504 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000022 DAVKWZ010000000
VERSION     DAVKWZ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30504
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.022"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            124..777
                     /locus_tag="VW854_001034"
     CDS             124..777
                     /locus_tag="VW854_001034"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEV0262282.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            787..1251
                     /locus_tag="VW854_001035"
     CDS             787..1251
                     /locus_tag="VW854_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV0262283.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1264..2532
                     /locus_tag="VW854_001036"
     CDS             1264..2532
                     /locus_tag="VW854_001036"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEV0262284.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAAGFSDDGIPLESSKIV
                     KEAMEEAKKFNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLG
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFKAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2546..2806
                     /locus_tag="VW854_001037"
     CDS             2546..2806
                     /locus_tag="VW854_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262285.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            2817..4178
                     /gene="pdrM"
                     /locus_tag="VW854_001038"
     CDS             2817..4178
                     /gene="pdrM"
                     /locus_tag="VW854_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEV0262286.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDKIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTARFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAILVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            4332..5300
                     /locus_tag="VW854_001039"
     CDS             4332..5300
                     /locus_tag="VW854_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEV0262287.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNVEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            5316..6308
                     /locus_tag="VW854_001040"
     CDS             5316..6308
                     /locus_tag="VW854_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEV0262288.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVILVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKD"
     gene            6526..7569
                     /locus_tag="VW854_001041"
     CDS             6526..7569
                     /locus_tag="VW854_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEV0262289.1"
                     /translation="MVDDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            7633..9318
                     /gene="lpdA"
                     /locus_tag="VW854_001042"
     CDS             7633..9318
                     /gene="lpdA"
                     /locus_tag="VW854_001042"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEV0262290.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHSAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVAVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            9382..10371
                     /locus_tag="VW854_001043"
     CDS             9382..10371
                     /locus_tag="VW854_001043"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEV0262291.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEITEYVFS
                     EEELAEINHIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(10444..11514)
                     /gene="xerS"
                     /locus_tag="VW854_001044"
     CDS             complement(10444..11514)
                     /gene="xerS"
                     /locus_tag="VW854_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEV0262292.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAKNIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVSDEQKNALDSL"
     gene            complement(12008..13558)
                     /locus_tag="VW854_001045"
     CDS             complement(12008..13558)
                     /locus_tag="VW854_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEV0262293.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALIGAICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
     gene            complement(13574..14353)
                     /locus_tag="VW854_001046"
     CDS             complement(13574..14353)
                     /locus_tag="VW854_001046"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEV0262294.1"
                     /translation="MATIKEIKELLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIVGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQSLSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            complement(14340..15191)
                     /gene="ylqF"
                     /locus_tag="VW854_001047"
     CDS             complement(14340..15191)
                     /gene="ylqF"
                     /locus_tag="VW854_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEV0262295.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETFRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            complement(15358..15561)
                     /locus_tag="VW854_001048"
     CDS             complement(15358..15561)
                     /locus_tag="VW854_001048"
                     /inference="COORDINATES: protein motif:HMM:NF012948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV0262296.1"
                     /translation="MQAGIPTDNEVSYALIYQQETLPATGSSTSALTALGLLAVGSLV
                     LLVRNSFLLPLKSHHLMAFFYIG"
     gene            complement(15613..21504)
                     /gene="iga"
                     /locus_tag="VW854_001049"
     CDS             complement(15613..21504)
                     /gene="iga"
                     /locus_tag="VW854_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin A1 protease"
                     /protein_id="HEV0262297.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGIGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYRKETIEIKIDFQEEI
                     QENPDLVEGIVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPIPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGAIVEPAIQPELPEAVVSDKGVPEVQPALSKAVITDKG
                     ETEVQPESPDTVVSDKGEPEQVAPLPEYKGNIEQVKPETPVEKTKEQGPEKTEEVPVN
                     PTEETPVNPNEGTTEGTSIQEAENPVQPAEESTTNSEKVSPDTSSENTGEESSNPSDS
                     TTSVGESNRPEHNDSKNENSEKTVEEVPVNPNEGTVEGTSNQETEKPVQPAEETQTNS
                     GKIANENTGEVSNKPSDSKPPVEESNQPEKNGTATKPENSGNTTSENGQTEPEKKLEL
                     RNVSDIELYSQTNGTYRQHVSLDGIPENTDTYFVKVKSSAFKDVYIPVASITEEKRNG
                     QSVYKITAKAEKLQQELENKYVDNFTFYLDKKAKEENTNFTSFSNLVKAINQNPSGTY
                     HLAASLNANEVELSTDDKSYIKGTFTGQLIGEKDGKHYAIYNLKKPLFENLSGATVEK
                     LSLKNVAISGKNDIGSLANEATNGTKIKQVHVDGVLAGERGVGGLLAKADQSSIAESS
                     FKGRIVNTYETTDSYNIGGLVGHLTGKNASIAKSKATVTISSNTNRSDQTVGGLAGLV
                     DRDAQIQDSYAEGDINNVKHFGRVAGVAGNLWDRTSGDVRHAGSLTNVLSDVNVTNGN
                     AITGYHYTGMKVANTFSSKANRVFNVTLEKDEVVSKESFEERGTMLDASQIVSKKAEI
                     SPLTLPTVEPLSTSGKKDSDFSKIAHYQANRALVYKNIEKLLPFYNKSTIVKYGNLVK
                     ENSLLYQKELLSAVMMKDDQVITDIVSNKQTANKLLLHYNDHSSEKFDLKYQTDFANL
                     AEYNLGNTGLLYTPNQFLYDRDSIVKEVLPELQKLDYQSDAIRKTLGISPAVKLTELY
                     LEEQFSKTKQNLGGSLKKLLSADAGLASDNSVTRGYLVDKIKNNKEALLLGLTYLERW
                     YNFNYGQVNVKDLVMYHPDFFGKGNTSPLDTLIELGKSGFNNLLAKNNVDTYGISLAS
                     HHGTTDLFSALETYRKVFLPNTSNNDWFKKQTKAFIVEEKSTIAEVKAKQKQAGTKYS
                     IGVYDRITSNTWKYRNMVLPLLTLPERSVFVISTLSSLGFGAYDRYRNSEHKAGKDLN
                     NFVEENASETAKRQRDHYDYWYRILDNEGREKLYRTILLYDAYKFGDDTTSGKATVEA
                     KFDSSNPAMKNFFGPVGNKVVHNHHGAYATGDGVYYMSYRMLDKDGAITYTHEMTHDS
                     DQDIYLGGYGRRSGLGPEFFAKGLLQAPDHPYDATITINSILKHSKSDSLEGSRLQVL
                     DPTERFQNSADLQNYVHNMFDLIYMLEYLEGQSIVKKLNVSQKMEALRKIENQYLTDP
                     ADGNEVYATNVVKELTEEEARNLNSFDSLIDNNILSAREYKAGTYERNGYFTIKLFAP
                     IFSALSSEKGTPGDLMGRRIAYELLAAKGFKDGMVPYISNQYEGIAKQNGQTINLYGK
                     KQGLVTDKLVLEKIFSGKYPSWTAFKKAMYQERIAQFDHLTKVIFKDPTKPWPSYGTK
                     TINNVDELQALMDQAVLKDAEGPRWSNYNPEIDSAVHKLKRAIFKAYLDQTNDFRSSI
                     FENKK"
     gene            complement(21713..22891)
                     /locus_tag="VW854_001050"
     CDS             complement(21713..22891)
                     /locus_tag="VW854_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262298.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKNGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
     gene            complement(23106..26756)
                     /gene="addA"
                     /locus_tag="VW854_001051"
     CDS             complement(23106..26756)
                     /gene="addA"
                     /locus_tag="VW854_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEV0262299.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAANELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKETFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQESDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FAKLGQLSDQITILDYQERYHQDTWELAKTFQTFMSHFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNKHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQVMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLESKEYPAAK
                     NGKLNSNTRLQARNFQDWLWVISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQIIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTALGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            complement(26753..30028)
                     /gene="rexB"
                     /locus_tag="VW854_001052"
     CDS             complement(26753..30028)
                     /gene="rexB"
                     /locus_tag="VW854_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEV0262300.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPAKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMNFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKISSDFNQIALVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLTNLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWNVFLKEGVVTKQLQDLTATMEAIEQERQAEVWKAFCHVL
                     EQFATVFAGSQISLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKIAQNTSLLTDEERQNINQATEEGVQLLIASSENLKKNHYTMLSLVNSA
                     RKQLVLSAPSLFNESESKESAYLQELVHLGFSRKEKRMNHKRLSKEDMGSYHSLLSSL
                     VAYHQQGETSDTEQDLTFVKVLARVMGKKLDQQGLENPALPTSPSSKQLTKDTLQALY
                     PAEQEFYLSTSGLTEFYRNQYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PDEDSFDNRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPVIE
                     TIKEEANFGGKEQAFIQLDNGRSVFVRGKVDRIDRLKADGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWLEKIREE
                     LNR"
     gene            complement(30228..30383)
                     /locus_tag="VW854_001053"
                     /pseudo
     CDS             complement(30228..30383)
                     /locus_tag="VW854_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
BASE COUNT         8578 a         6099 c         6305 g         9522 t
ORIGIN      
        1 ctgtattcac gtattcaaat cgtatatata gtcgatttat taaatcttta gataaactgt
       61 aaaaagtggt ttccatagcc actttttgct ataatagagg cagtaaaaat gaaggagtcg
      121 gctatggaac actcgtcttg gcatgctttg attaaggcgc aattacctga gggttatttc
      181 gggaaaatca atcagtttat ggagcaggtc tattctcagg ggattattta tccacccaag
      241 gaaaaggttt ttcaggctct cttgacaaca ctgcttgaag aagttaaggt ggtaattcta
      301 gggcaagacc cctatcacgg accaggtcaa gcgcagggct tgagtttttc tgtacctgac
      361 tctatcccag ctccaccatc cttgcaaaat atcttgaaag aattgtcaga tgatatcgga
      421 gttaagaaat ctcatgattt gacagcttgg gctgagcaag gagtcttgct tcttaatgct
      481 tgtttgactg ttcctgctgg acaggccaat ggtcatgctg gtcaaatctg ggagcctttt
      541 actgatgctg tgattcaggt ggtcaatcat ctagatagac cagtcgtttt tgtactctgg
      601 ggagcttatg cacgtaagaa gaaggcatta gttaccaatc ctcatcactt gattatcgaa
      661 tcagcccatc caagtccttt gtcggtttat agaggatttt ggggttccaa gcccttttcc
      721 aaggccaatg cattcttaaa agagacagga caagagccaa tcgattggct tagataagga
      781 gaaaatatgc ctcagttagc gacgatttgc tacattgata atgggaaaga actgctcatg
      841 ctccatcgta ataagaaacc caatgatgtc catgaaggga aatggattgg tgtgggtggt
      901 aagctagaga gaggagagac gccccaggaa tgcgcggcgc gtgaaatcct tgaagaaaca
      961 gggctcaaag ccaagccagt tctaaaaggt gtcatcactt ttcctgaatt tacaccagat
     1021 ttagactggt acacctatgt ttttaaggtg acggagtttg agggcgactt gattgactgc
     1081 aatgagggga cgctagaatg ggttccctat gatgaggttt tgagcaagcc gacttgggaa
     1141 ggtgaccaca cctttgttga gtggctttta gaggataaac ccttcttttc agccaagttt
     1201 gtttatgatg gggataaatt gttggatacc caagttgatt tctatgaata aaggagaaag
     1261 cagatgctac taatcaaaaa tggtcgtgta atggatccca agtctggttt ggatcaagtt
     1321 tgtgatgtct tagttcaaga tgggaaaatt atcaaaattg cgcctgagat cacggaagaa
     1381 ggagcagaaa cgattgatgc tactggtctt gtggttgctc ctggtttggt cgatattcat
     1441 gtgcatttcc gtgaacctgg tcaaacacat aaagaagaca ttcatactgg tgccctggca
     1501 gccgctgcag gtggttttac tactgtcgtc atgatggcta ataccagtcc aaccatttca
     1561 gacgtggaga ctttgcaagc agttctccag tcagctgcca aagagaagat taatgtcaag
     1621 acagttgcga ccattactaa aaactttaat ggtaaaaact tgactgactt taaggcgctc
     1681 ttagaagctg gtgcggctgg tttctctgat gacggtattc cgcttgagag cagtaagatt
     1741 gtcaaggaag ccatggagga agccaaaaaa ttcaatacct ttatcagcct tcatgaggaa
     1801 gatccaggtt tgaacggtgt tcttggcttt aatgaaaata ttgctagaga acatttccat
     1861 atctgcggtg ctactggagt ggctgagtac gctatgatgg cgcgtgatgt catgattgcc
     1921 tatgcaacta aagcccatgt tcacatccag catttgtcta aggaagaaag tgttaaagta
     1981 gtggagtttg ctcaggggtt aggtgcagaa gtcacagcag aagtagcacc acagcatttc
     2041 tctaagacag aagcacttct tttaacacaa ggtagcaatg ctaagatgaa tccaccgctt
     2101 cgtttggaat cagaccgtcg tgccgttatc gaaggtctca aatcaggtgt catcacagtt
     2161 attgcgactg accacgcgcc tcatcatgta gatgaaaaaa atgttgaaga tattaccaaa
     2221 gcgccatctg gtatgactgg cttagaaaca tccctgtctc tcggcttgac ctatttggta
     2281 gaagctggtg agttgagctt gatggaatta cttgaaaaaa tgacatacaa cccagccaag
     2341 ctttacaact ttaaagcagg ttacttggct gagaatggtc cagcagatat cactattttt
     2401 gatgccaagg ctgaccgcct tgtggactcc cattttgctt ccaaagcagc taattcacca
     2461 ttcatcggtg aaaccttaaa agggcaggtt aaatatacca tctgtaaggg acaaatcgtc
     2521 tatcaagctt gataggagtt agaatatgaa ttattttcga aaacgtaggg agagacaagc
     2581 gaaatctaat agtggaattt actgtcccgc agctaatcgt tccaaaattc aatatgaaac
     2641 taaagaaaaa gctgaccatg cggttcaata tgataacgga ggtttagtta gaagctatta
     2701 ttgtcgtaca tgtgcgtgtt ggcatactac ttcaaaatct aataaacctc cgctaagttt
     2761 gaaaaactat ggtctgaaac tctttggttt agataaatca gaatagaaag agaagtatgt
     2821 ataagacaaa gtgtttacga gagaagttag tattattttt aaaaattttc ttcccaatcc
     2881 tgatctacca atttgccaat tattctgcct cttttgttga tactgcaatg acaggtcaat
     2941 acaacactat ggacttggct ggtgtatcta tggcaaccag tatctggaat cctttcttta
     3001 catttctaac agggattgtg tcagccttgg tgcctatcat tggtcaccat cttggtcgag
     3061 gcaaaaagga agaagttgcg tctgattttt accaatttat ttatttggcc ttgggcctat
     3121 ctgtggtctt gctggggatg gtacttttct tggcaccaac aatcttgaat catattgggt
     3181 tagaagcagc agtagcggca gtagcggttc gctatctttg gtttttatct atcgggatta
     3241 tccccttgtt gctctttagc gtcattcgtt ccttactgga ttcgctgggc ttgaccaaac
     3301 tgtccatgta cctcatgctt ttgttactcc ctctcaatag cggatttaac tatctcttga
     3361 tttacggtgc ctttggtgtt ccagaactgg gaggggctgg tgctggttta ggaacatcct
     3421 tggcctactg ggtcttgctt gggatttctg ttctggtttt atttaaacag gagaagctca
     3481 aagccttaca ccttgagaaa cgaattccac ttaatatgga taaaattaag gaaggagttc
     3541 gtttaggtct gcctattggg ggaactgtct tcgcggaagt ggctatcttt tcagtggttg
     3601 gcttgattat ggctaagttt tcgtccttga ttatagctag tcaccagtca gctatgaact
     3661 tttcaagtct tatgtacgcc tttcctatga gtatctcatc ggctatggct attgtcgttt
     3721 cctatgaagt gggagccaag cgatttgatg atgcgaaaac ctatattggt ctaggaagat
     3781 ggactgccct catttttgcg gccttcacct taaccttcct ttacattttt agggggaatg
     3841 tggccagtct ttatggtaac gacccaaaat ttattgattt gacagcgcgt tttttaactt
     3901 atagtctttt cttccagtta gcagatacct ttgcggcacc gcttcaggga attttgcggg
     3961 ggtataagga tacagttatt cctttttacc ttggtttgct tggttattgg ggcgtagcaa
     4021 tccttgtggc tacgctattt gattccctaa cagattttgg agcttattct tactggatcg
     4081 gcttgattat tagtttgatt gtgagtgggg cgctctaccg ttggcgttta actgtgatta
     4141 tgaagagatt tgaatcttta gcaaaatcca aacgctaaaa agtttgtgaa aaaatattct
     4201 tgataagaaa atcccttgtt ttcacggggt tttctttttt attttgtgaa atttctttta
     4261 gaaaaacttt gacagtattt gccaaaaacg gtaaaatagt aaggtaagaa gaaagttaga
     4321 aaggcaagaa gatgtcaact ttagataaaa atcttttgct agaaatgttc cgtaagatgg
     4381 aagaaatccg tcgcatggac ttaaaaattg cacaattagt aaagaaaggg aaagtgccag
     4441 gaatgacgca cttttctgtt ggtgaagagg cagctaacgt gggggctatg ttagctctca
     4501 atccagatga tctgattacc tcaaaccacc gtggacacgg gcaagctatt gctaaaggga
     4561 ttgacctcaa cggaatgatg gctgaaatcc ttggtaaata cactggaacc tgtaaaggga
     4621 aaggtggatc tatgcatatc gccgaccttg atgctgggaa ccttggtgcc aatggtatcg
     4681 taggtggtgg tatggggatc gctgtcggtg cagccctcag tcagcaaatg caaaataccg
     4741 gtaaaatcgt tgtctgcttc tttggagatg gtgcgaccaa tgaaggtgtt ttccacgaag
     4801 cagtgaacat ggcttctatc tggaacctgc cagtcatttt ctattgcatt aacaacggtt
     4861 acggtatctc tgcggatatc aagaaaatga ccaatgtaga acatatccat caacgtagcg
     4921 ccgcttatgg aattcctgga atgttcatcg aagacggtaa caatgtcatc gatgtctatg
     4981 aaggatttca gaaagctgta gaccatgttc gcagtggcaa tggtccagtc ttgattgaaa
     5041 gtgtaactta tcgctggctt ggtcactcat catctgaccc tggtaaatat cgtacgcgtg
     5101 aagaagtgga attgtggaaa caaaaagatc caatcgaaaa cctccgcaat taccttattg
     5161 aaaataacat tgcaagtgcc gaagaattgg aagaaatcca agcgcaagta aaggaagcag
     5221 tagaagcttc tgttaaattt gcagaggaaa gcccattccc accgcttgaa tcagcctttg
     5281 aagatattta cgcagactaa ggagaaagag ataaaatgga aacaaaaaca atgtccttcc
     5341 gtgacaccat tatccttgct atgtctgagg aaatgcgtcg cgatgaaaat gtgttcttga
     5401 tgggagaaga cgtcggtgtc ttcggaggag acttcggtac ttctgttgga atgcttgaag
     5461 aatttggtcc agaacgtgtc cgtgactgtc cgatttctga agctgccatc tctggagctg
     5521 cagcaggagc agccatgaca ggacttcgtc caatcgtcga tatgaccttc atggacttct
     5581 ctgttattgc catggacaat atcgtcaacc aagctgctaa aacacgttac atgtttggtg
     5641 gtaaaggtca ggttccaatg actgttcgtt gtgcagctgg taacggagtt ggctctgccg
     5701 cccagcactc acaatctcta gagtcttggt ttactcacat tccaggactt aaggttgtgg
     5761 cacctggaac acctgcggac atgaaaggcc tgcttaagtc ttctatccgt gataacaacc
     5821 cagttattat acttgagtac aagtcagaat ttaaccaaaa aggggaagtg ccagttgatc
     5881 cagactacac aattccactt ggggttggtg aaatcaaacg ccaaggaacg gatgtaacag
     5941 ttgttactta tggaaaaatg cttcgccgtg tggttcaagc tgctgaagaa ttagcagaag
     6001 aaggaatttc agttgaaatt gttgacccac gtacccttgt tccgcttgat aaggatatca
     6061 tcataaactc agtgaagaag actggtaagg ttattctggt caacgatgcc cacaaaacaa
     6121 gtggctatat cggtgaaatt tcagctatta tttcagaatc agaagcattt gactatctag
     6181 atgcaccaat acgccgttgt gcaggagaag atgtgccgat gccttatgca caaaacctag
     6241 aaaatgcaat gattccaaca gttgaaagta tcaaagatgc aatccgaaaa acttataaca
     6301 aagactagag ttacataata taagttatgt aaataaaata aagctgacga gaaactttgt
     6361 atcttttagg tgcagagctc ttcgtcatta atatcttaga ttagagcacg ggctaaaagc
     6421 tcggaaaaaa gataaatctc cttggcttca tcgaagccat cgtcgatttc ctatttttca
     6481 gtcgcttttg acgccctttg tatcataaat agaattggag aggtcatggt tgatgacaag
     6541 ctaagagcga ctcctgcagc tagaaagtta gcggatgatt tagggataaa cctctacgac
     6601 gtttctggct caggtgcaaa cggtcgtgtc cacaaagaag acgtggaaac ttataaagac
     6661 acaaacgtgg ttcgcatttc gccacttgca aaacgaattg ccctcgaaca taacattgct
     6721 tggcaggaaa tccaaggaac cggtcatcgt ggtaaaatca tgaagaagga tgttttggcc
     6781 ctgcttcctg aaaatatcga aaacgatagc atcaaatctc ctgctcagat tgaaaaagtg
     6841 gaagaagtcc ctgataacgt aacaccatat ggtgaaatcg agcgtattcc aatgacacca
     6901 atgcgtaagg ttattgccca acgcatggtt gaatcttact tgactgcgcc aaccttcacc
     6961 ctcaactatg aagttgatat gactgaaatg ttggctcttc gtaagaaggt tcttgagcca
     7021 atcatggaag caactgggaa gaagactact gtaacagacc ttctttcgct tgcagttgtt
     7081 aagactctta tgaaacaccc atacatcaat gcttcattga cagaagatgg caagactatt
     7141 atcactcaca actatgtcaa tcttgccatg gcagttggga tggataatgg attgatgaca
     7201 cctgttgttt acaatgctga gaagatgagt ctttcagaac tggttgtagc ctttaaggat
     7261 gttattggtc gtaccttgga tggtaaattg gctccaagtg agttacaaaa ttcaacattc
     7321 acaatcagta atttgggaat gtttggtgtt cagtcctttg gcccgattat taaccaaccc
     7381 aactcagcta tccttggtgt cagttcgaca atcgagaagc cagttgtcgt caatggtgaa
     7441 attgtgattc gcccaatcat gagtttagga ttaaccattg accaccgtgt cgtagatggt
     7501 atggctggtg ctaagtttat gaaggacttg aaagagttga ttgaaacccc aatctcaatg
     7561 ttgatttaag agcaagagta ttcattttta agatatagat aaaggaagga agacatggcc
     7621 ttagaagtaa ttatgccaaa agccggcgtg gatatgacag aaggacaaat cgtccaatgg
     7681 aataaaaaag tcggagaatt tgtaaaagaa ggagaaatcc ttttggaaat catgactgat
     7741 aaagtcagca tggaattgga agccgaagaa gatgggtact tgattgccat tctcaaagga
     7801 gatggtgaaa ctgtccctgt aacggaagtt atcggttacc ttggtgaaga aagggaaaac
     7861 atcccaacag ctggagcagc atcgccagaa gctagccctg tacctgtagc aagtacctca
     7921 aacgatgatg gtaagagcga tgatgctttt gacatcgttg tgattggtgg aggtcctgct
     7981 ggttatgttg cagccattaa agctgcccaa ctcggcggta aggttgccct tgttgagaaa
     8041 tctgaacttg gtggaacctg cttaaaccgt ggatgtattc caaccaagac ctaccttcat
     8101 agcgctgaaa ttattgaaaa tatcggtcat gctgcaaatc gtggtatcgt gattgaaaat
     8161 cctaacttca ctgttgatat ggaaaaactt ttagaaacta aatctaaagt tgttaatact
     8221 cttgttggtg gtgttgcagg acttcttcgt agctacggag ttgctgttca taaaggaatt
     8281 ggtacaatca ctaaagacaa gaacgtcttg gtaaatggtt ctgaattgct tgaaaccaag
     8341 aaaatcattc ttgctggtgg ttcaaaagtc agcaagatca acgtccctgg tatggaatct
     8401 ccacttgtca tgactagtga tgacattctt gaaatgaacg aagtgccaga aagccttgtt
     8461 atcatcggtg gtggagttgt cggtatcgaa ctcggtcagg ccttcatgac atttggttca
     8521 aaagtgactg ttatcgaaat gatggaccgt atagttccag ctatggatgt ggaagtttct
     8581 aagaaccttc gcttgatcct tgagcgtaaa ggaatgacca tcttgactgg tactaaactg
     8641 caagaaatca ttgaggaaaa tggtcaactt cgtatcaagg ttgaaggaaa agacgatatc
     8701 atcgcaagca aagctcttct ttcaattggt cgtatgccag accttgaagg tattggagag
     8761 gttgagtttg aattggatcg tggttgtatc aaggtcaacg aatacatgga aacttcagtt
     8821 ccaggcattt atgcaccagg tgacatcaac ggtactaaga tgttggctca cgcagctttc
     8881 cgcatgggtg aagtttccgc tgaaaatgcc cttaaaggaa atcatgcagt tgccaaattg
     8941 aatttgactc ctgcagccat ctacactctt cctgaagtag cagcagtagg tttgacagaa
     9001 gaacaagccc gtgagaaata cgatgttgcc atcggtaagt ttaactttgc tgctaacggt
     9061 cgtgctattg catctgacgc agctcaaggt ttcgtaaaag ttatcgctga taagaaatac
     9121 ggagaaatcc ttggtgtaca cattattggt cctgcagccg cagaattaat aaacgaagca
     9181 tcaagcatca tcgaaatgga aatcactgtt gaggaaatgc tgaagaccat ccacgggcac
     9241 ccaacttact ctgaagtgat gtacgaagcg tttgcagatg ttctaggaat ggccatccat
     9301 tcacctaaga aaaaataaaa cgaagataga ttagactgga aggttatttc cagtttctat
     9361 catataaagt aaagggatat catgaaatac attatcaatc attcaaatga cactgctttt
     9421 aatattgcct tggaagaata cgcctttaaa caccttttgg atgaggatca aatcttccta
     9481 ctttggatta acaagccatc tatcattgtt ggtcgtcacc agaacactat cgaagaaatc
     9541 aaccgtgatt atgttcgcga aaatggtatt gaggtagttc gccgtattag tggtggtgga
     9601 gctgtttacc acgatttaaa taacctcaac tacacgatca tctccaaaga agatgaaaat
     9661 aaggcatttg acttcaagag cttctcaact ccggttatca ataccctggc tcaacttggg
     9721 gttaaagctg agttcacagg tcgtaatgac cttgagattg atggtaagaa gttctgtggc
     9781 aatgcccaag cctatatcaa cggccgtatc atgcaccacg gttgcttgct ctttgacgtt
     9841 gatttgtcag tcctcgcaaa tgcccttaag gtttcaaaag ataaatttga atcaaaaggt
     9901 gtgaaatccg tccgtgcccg tgtaaccaat attatcaatg aattaccaaa aaaaatcaca
     9961 gtcgaaaaat tccgtgattt actcctggaa tacatgaaaa aagagtaccc agagattact
    10021 gaatacgtct tttcagaaga agaattggcc gaaatcaatc acatcaagga tactaagttt
    10081 ggaacttggg actggaacta tggtaaatca cctgaattta acgtccgtcg tggaaccaaa
    10141 ttcactagtg gtaaggttga agtctttgct aacgttactg aatcaaaaat ccaagacatc
    10201 aagatttatg gtgacttctt tggtattgaa gacgttgctg cagtagaaga tgtccttcgt
    10261 ggggtgaaat acgaacgcga agatgttctt aaggcgctaa aaaccattga tatcacacgc
    10321 tacttcgctg gtattagccg tgaagaaatc gctgaagcgg tagttggata aatcaaaaag
    10381 aagttggcca actaaccaac ttcttttttt gatatttatt tacataattt aaaatacgta
    10441 aaatcataaa ctatccagag cattcttttg ttcatcacta acaatatggg tatagaggtc
    10501 agtgacttgt gtgctagcat gtcctagttg gtgactgact aaaacttgtg atttagtcgc
    10561 atcatagagc ctagttgcta gtgtatggcg cagtttatgg ggtgttacac gcactttaaa
    10621 atcctctgag tatttagcaa ccattttctc aacgctagaa gcatcgatac gattaggaac
    10681 acctctgtag agagttaaaa aaagggctgt atctgttttt tccgttttat agcgttgatt
    10741 ccgaatggcc agataattct ctaaataagg tttagcaaaa gcagcgacat tgactgagtc
    10801 acgtttgcca ccttttcgag taacatcaat aaccatcatt tttagattga gatctcttag
    10861 atctagatta acagcttcag ataagcgaac accagatgcc aagagaaggg caataatggc
    10921 taaatctcgt tctttatttt tgttgaatga tgagagagct cgatttgaaa gctgttgtgg
    10981 gtgctcttga tcgatataag ttagaaaacc ttctgtttca tcacctagaa agagtttttg
    11041 cttgatattt ttagctctgg cagcaagggt ttctttcttt ttcttggttg aaactttttt
    11101 cattacatta cgatagaaat aaggttcccc ctgatcgttt tcaacctcct cggttagata
    11161 cttgtaaaga ctagaaagtg ctgataaggt tcgattgata gttgtctgtg aaacaccttg
    11221 ttttgttgta ttagcattca gcaagggacg ttcacgtaga taaaggataa aggattccat
    11281 gtctttctta gacatatttt ccaaaactga taaaggaata tcggatattt tatcagcgtt
    11341 tgaaatacca gactccaaaa cccagctgaa aaatcggtca tattccttaa ggtattcgta
    11401 cagggttgta aaactgtagg gcacagccag cttagattgg tagtattcca gaacatacca
    11461 gggcatgagt tgttttagtt tgtcgattcg ttccagtaaa atctcacgtt tcatgtattt
    11521 tctccataag taagtatact agtagtatag catagacttg tatatttttc aagaaaatta
    11581 tagtttttca gaaagatgtt ataggagttt ttcaaagtga taaaaagact tagacctaca
    11641 aacaaaacag aataaggaaa ataataaaaa tctcacccac taaacttctg ttaatactac
    11701 atagctagaa attgatttga ttgtcctgat tgttttatcc tatttctatt tcatttcact
    11761 atataattgc tattgttttc tttttcatga ttttaatctt actgtacaga tttgatacag
    11821 taaactcctt tctagcttta tgctttatca tttctaactt aattatagtc ttatttttaa
    11881 taaagttcaa ttatttatat gtaaaattcc tcatcagtaa aaaataaccg atctcatttc
    11941 tgagaatcgg ttttctaaat aaatcaagct gagcatttac ataacataaa ttatgtaaat
    12001 gattgtacta caacaacaaa tctttgactt ttccaatttc actttttgga ataaccaggt
    12061 gaatcatatc acccagatac attctggttg agccgttaac tgtttggctc ttgccattat
    12121 ggacttgagt tgtgatgagg acgttgtgtg gtaagttgag ttcatgaact tgtttcccag
    12181 caattttatc agaaactggt atttcgataa gtgtaacttc tccttcgcta gatacttctt
    12241 ctggaagcat tttttccagc atggcttcat agactggcgt acctttgagc aaatccatga
    12301 taatataaga aacaagagtg acaagaccaa gtggcatgag gttgcgaata tctcctacca
    12361 tctcagttac gaggatcata gcggttaagg gagcttttga tatggctcca aaatagccac
    12421 tcattcctag aatgacaaat atagggaatt gctcttgact gacaagtcca agattgacac
    12481 agatagcacc aattaaggca ccaagcaaag aaccaagagc taaaatgggg aggaaaattc
    12541 ctcctggcag tccacttcca tagctaatca tactccaaat aaagcggatc aaaaagtaag
    12601 ctaataaaac ttggaaacta aaattttgtt cagttaaaga aaggacaagc tgatttccgc
    12661 caccaatgat ttgaggtaag aagattccga ctggtatgat aaggataaaa gccaagatgg
    12721 gataataagc tctatccaaa tggatttttt gaccaatcaa gtcataaact cttccaacgt
    12781 ttaatacagc tttctcatag agaaagcctg aaaatccaag gaaaattccc atgacgagat
    12841 atatccaata ctgatctagg gtcatgggag gaatgttatc tggcatatcc aatactggtg
    12901 tcaaaccgaa catgagtaga gacacaaagt ttgctacgat gctggctgct agagttgaga
    12961 cccagaaaaa gcgcgaaaaa tggtgataga cttcttctac aacaaagaga agtgctgcaa
    13021 taggagcatt aaaggctgcg gctaaacctg ctgcagctcc actggcaatc aaggaacgtt
    13081 cctctactgg actggatttg agccacttgg caattccttt accaccaact gctccaagtt
    13141 gaatactggg tccctctcga cccagcatga gtccactggc aatagcaaga atacctagta
    13201 catatttttt ccaaagaatg ccccaccagt tgagggacat gaggcctttc agttcggctt
    13261 cgacttgagg aattcctgag cctttaatat ctttttctga ccgtgttagt ttggcactga
    13321 gccaacagat gagtatataa aacaaaacca gtacaaaaag attgcgcact aagtaccctt
    13381 gatcttgata aactccttgt atcaggtgga agcccttttc aattaagaaa cgaaaggatc
    13441 cgacgataat tccaacgatg aggccgacaa tgattcctcg accaacttgg gatagtatag
    13501 tgctggatgc aaaggcgaat tctttcttag aacggactat ttccgactgt tcctccatgg
    13561 tatttccttt caattaactt tcttttttac ctaaaaccag tgatttaacg ggttcaaagc
    13621 tggttcggtg aattggggta actcctagtt ttgtgaggcc ttccagatgt ttagctgtgc
    13681 catatcctgc attagtagcg aaatcatagc cagggaactg ctgatcgtat tccttcatca
    13741 attcatcacg tgttaccttg gctactatag atgctgctgc gatagagagg gagttggcat
    13801 ctcctttgat aatggaggtt tgtgaaatgg gcaagtccag tttcatggca tcaatcaaaa
    13861 ggtgctctgg ttgagggctg agctgggaga ttgcttcttg catggctagt ttggttgctt
    13921 catagatgtt gacttggtcg atgacctgat tatctatgat accaattcca atcgacaagg
    13981 attggtcttg aacggcttgg aaaatctcca gatgtttctt tttaggaatt ttcttgctgt
    14041 cgttgagacc tttaatctta caatttttag gtaaaatgac ggccgcagcg actacaggac
    14101 cagcaagagg accacgacca acctcatcaa tacctacaat taaggtcaat ccttgcttat
    14161 aaagttcttt ttcataagaa agcatggatt ccaagcgcaa attttcatct aattcagctt
    14221 gaatggctct tttacgcttg ctgatttcct tttgaactcc tgagcgatta tccttttcaa
    14281 gctctaaaaa aatagggctt tctaactcct tgactgtcac aaggagttct ttgatttctt
    14341 taatcgttgc catcgaggtc ttccaatgta tctaaggtat agttaccgag tttgccatca
    14401 cggacttcct tcacgaagag actgtaaaaa cggtcatagt catcacggaa accgagggcg
    14461 cgggtcatat ccataataat cacaggcgct tcttcttcaa ttttcatttg tttgaagcgt
    14521 tcagccagct tttctggata atgttctttg aaataattga taccaaaaat ggtaacctca
    14581 tccataggaa gcaactgatc cttgatagct ccagtcaatg ccaacttaag tgcaacagtt
    14641 tcatcctcaa acttaggcca gagaatcccc ggtgtatcca agatttccag gtctttattg
    14701 gttttaagcc attgttgacc ttttgtgacc cctggcttgt ttccaacaac agcaatcttt
    14761 ttaccagcca aacggttcat cagagttgat ttaccagcgt ttggaatccc gataatcata
    14821 gtacgaaagg tttcaatctg aatcccacgt tctttctggc gagcaatctt atcagccatg
    14881 agcttcttgg ccgcatctgt tacaactttt acagtcactt gctctttgga gttgatagct
    14941 agcgtctgga ttccttgtga ttcaaaatac tgacgccatt ccttggtcat tgctggatca
    15001 gccaagtcgg ccttgtttaa aatcaagagt tttggtttat caccaacaat cttggtcaac
    15061 ataggatttt gactagatag aggcaagcgt gcatctacta aaatcgtcac aaaatcaaca
    15121 aattttaaat tctcctgcac ctgtcgacga gctttagaca tgtgaccagg aaaccattga
    15181 atagtagcca ttgagttttt tcctttcgta gctctgaagc tgttaatcta gaactaagag
    15241 atctcaatct aaaaatgaat gatgtccaaa cagttcttgt tttatgacat aaaaatctac
    15301 tttatattat atcatgcctt ctttattttg tccgatgata gccttttacc ctatctttta
    15361 ccctatatag aaaaaagcca tcaaatgatg acttttcaga gggagcaaaa aactgttccg
    15421 aaccaaaaga actaaactac caacagctaa taggcctaaa gctgtaagcg cagaagttga
    15481 tgaacctgtt gcaggaagag tttcctgttg ataaataaga gcataactta cttcattatc
    15541 agtaggaatt cctgcttgaa tcttggtttg ttcttcatca cctttttaaa ctttatttcc
    15601 taatagtaaa cactattttt tattctcaaa aattgaactt ctaaaatcat ttgtttggtc
    15661 aagataggct ttaaagattg ctctcttcaa cttatgaacg gcactgtcga tttctggatt
    15721 ataattactc caacgtggac cttcagcatc cttgagaaca gcttggtcca tgagggcttg
    15781 caattcatcc acattattga tagtctttgt gccatagctt ggccatggtt ttgtcggatc
    15841 tttaaaaata actttagtca aatgatcaaa ctgagcaata cgctcttgat acatagcttt
    15901 tttaaaagct gtccaagatg gatacttgcc agagaatatc ttttccaaaa caagcttatc
    15961 tgtcactaat ccttgctttt taccgtaaag gttgattgtc tgaccatttt gtttagcaat
    16021 tccttcatat tggttggaaa tatatggtac cataccatct ttaaatcctt tagcagctaa
    16081 gagttcataa gctatccttc gtcccataag atctccagga gtacctttct cactgctcaa
    16141 tgctgaaaag attggtgcaa agagttttat agtaaagtag ccatttctct catatgtacc
    16201 agccttatac tcacgagctg aaagaatatt attgtcaatc aaactatcaa aactattcag
    16261 gtttcgggcc tcttcttctg tcaattcttt gactacgtta gtagcgtaaa cctcatttcc
    16321 atcagcagga tctgttagat attgattctc gatttttctg agagcttcca ttttctgaga
    16381 aacattcagt ttcttaacaa tcgattgccc ttctagatat tccaacatat agatgaggtc
    16441 aaacatgttg tgaacatagt tttgaagatc tgctgagttt tggaatcttt ctgtcggatc
    16501 caagacttgc agacgcgatc cttctaagct atctgatttt gaatgtttca agatagagtt
    16561 aatggtaatg gtcgcatcat atggatggtc tggcgcttgt aataaacctt tagcaaagaa
    16621 ctcaggacca agaccacttc ttcgaccata gccgccaagg taaatatctt gatccgaatc
    16681 atgggtcatc tcatgggtat aggtaatggc tccatccttg tcgagcatgc ggtaagacat
    16741 atagtagaca ccatctcctg ttgcgtaggc accgtgatga ttatgaacaa ccttattacc
    16801 aactggacca aagaaattct tcatggctgg attggagcta tcaaacttag cctccactgt
    16861 agcttttcca gatgttgtgt catcaccaaa cttataggca tcataaagga gaatcgtacg
    16921 atatagtttt tcacgtccct cattatctaa aatgcgatac caataatcgt agtgatctcg
    16981 ctgacgtttg gcagtttcac tcgcattttc ttcaacgaaa ttattgaggt ccttgcctgc
    17041 tttatgttca ctattacggt agcgatcgta agcaccaaat cctagactag acaaggttga
    17101 gatgacaaat acagatcttt ctggcaaggt aaggagaggc aagaccatat tccggtactt
    17161 ccaagtatta ctagtaattc ggtcataaac accgatagag tacttagttc ctgcttgctt
    17221 ttgcttggct ttgacctctg caatagtgga tttttcttcg acaatgaaag ctttggtctg
    17281 ttttttaaac cagtcattgt tgctagtgtt tggtaaaaag acttttcggt aagtttcaag
    17341 tgcgctaaac aaatctgtcg ttccatgatg gctggctaga ctgataccat aagtatcgac
    17401 attgttcttg gcaagaagat tgttaaagcc agatttacct aactcaatca gagtatctag
    17461 tggggaagta tttcctttac caaagaagtc tggatgatac ataactaggt ctttgacatt
    17521 cacttgacca tagttaaagt tataccaacg ttctaaataa gttaaaccga gtagcaaggc
    17581 ttccttatta ttcttgattt tatctacaag atagcctctg gtgactgagt tatcgctagc
    17641 tagaccggca tctgctgaca aaagtttttt caagctgcct cccagatttt gttttgtttt
    17701 ggagaactgc tcttctaaat agagctcggt taacttaact gctggagaaa taccaagtgt
    17761 ctttctgata gcatctgact ggtaatcaag cttctgcaac tcaggcaaga cttccttaac
    17821 aatagagtct cgatcatata agaattggtt aggagtgtag agaagtcccg tattacctag
    17881 attatattct gctagattgg caaaatcagt ctggtacttg agatcaaatt tctcagatga
    17941 atggtcatta tagtgaagta agagtttgtt tgcagtctgt ttgttggaaa caatatctgt
    18001 gattacttgg tcatccttca tcataactgc agataagagt tctttttggt ataagagact
    18061 gttctcctta accaggtttc cgtatttcac gatggttgac ttattataaa aaggtaacaa
    18121 tttttcaatg ttcttataaa ccaaagcacg gttagcttga taatgggcta tcttagaaaa
    18181 atcactgtct ttcttgccac ttgttgagag gggttccacc gttggtagag tgagaggact
    18241 tatttctgct tttttgctta caatttgaga agcatctagc attgttcctc tttcttcaaa
    18301 ggattccttg ctgacgacct catccttctc taaagtgaca ttgaaaactc tattagcctt
    18361 actactaaat gtattagcta ccttcattcc tgtatagtgg tatccagtga tggcatttcc
    18421 gtttgttaca ttaacatcgc tgagaacatt ggtcaaactt ccagcatgcc taacatcacc
    18481 agaagttcga tcccacaaat tgcctgccac tccagcgact ctaccaaagt gcttgacatt
    18541 gttgatatca ccttcagcat agctatcttg gatctgtgca tctcggtcta ctagacctgc
    18601 aagaccaccg acagtctgat ctgacctatt ggtgtttgac gaaatggtta ctgtcgcttt
    18661 ggatttagca atagacgcat tttttcctgt taaatgaccg accagaccgc caatattgta
    18721 ggaatcagtc gtttcatagg tattgacaat tcttcccttg aaactgctct ctgcgatgct
    18781 tgattggtct gccttagcca acaaaccacc gacaccacgt tctccagcca gaacaccatc
    18841 aacatgaact tgtttaatct ttgtgccatt cgtagcttca tttgccagtg aaccaatatc
    18901 atttttccct gaaatagcaa cattttttag actcagtttt tctactgtag caccactcaa
    18961 gttttcaaaa agaggctttt tcaagttgta aatagcataa tgcttaccat ctttttcacc
    19021 aatcaactga ccagtaaagg ttcccttgat ataggattta tcatcagtac taagctccac
    19081 ttcgttagca ttcaggctgg ccgctaaatg ataggttcca gagggatttt ggtttatagc
    19141 tttgaccagg ttgctaaagg aagtaaagtt tgtattttcc tctttagcct tcttatcgag
    19201 gtagaaggtg aaattgtcga catatttatt ttctagttct tgctggagtt tctcagcttt
    19261 ggctgtgatt ttataaactg actgcccatt tcttttctct tcagttattg aagctactgg
    19321 tatatagaca tctttaaatg ctgaagattt taccttgaca aagtaagtat ccgtattctc
    19381 tggaattcca tccaatgaaa catgttgtct gtaggttcca ttcgtctgac tatacaactc
    19441 aatatcagaa acatttctta attcgagttt tttctctggt tctgtttgtc cattctctga
    19501 tgttgtatta cctgaatttt ctggttttgt tgcagttccg tttttttctg gttgatttga
    19561 ttcttcaact ggtggttttg aatcactagg tttattggat acttctccag tattttcgtt
    19621 agctattttc ccagagtttg tttgtgtttc ttctgcaggt tgaactggtt tttctgtttc
    19681 ttgatttgag gtaccttcta ctgtgccttc atttggattt actggaactt cttctacagt
    19741 tttttctgaa ttttcatttt tagagtcatt atgttctggt ctatttgatt ctccaactga
    19801 ggttgtcgaa tcactaggat tactggactc ttccccagta ttttcgctag atgtatctgg
    19861 tgatactttc tctgaattcg ttgttgattc ttctgcaggt tgaactggat tttctgcttc
    19921 ttgaattgag gttccttctg tagtaccttc atttggattt actggtgttt cttctgttgg
    19981 atttactgga acttcttcag ttttttctgg accttgttct ttggtcttct caaccggagt
    20041 ttcaggtttt acttgctcaa tattaccctt atattctgga agcggtgcta cctgttcagg
    20101 ttcaccttta tcacttacca cagtatctgg cgactctggt tgaacctcag tctcaccttt
    20161 gtcggttata actgctttgg acaacgctgg ttgaacttct ggtacgcctt tgtcacttac
    20221 tacagcttcg ggcaactcag gctgaattgc gggttcaaca atagctccag actgtacgtc
    20281 cttatgttct acaccagtct caggttgttc ctttataact tgagtttttt tagtaccttt
    20341 ttcgactatt cttggaatag gcgcagtcgt tgaagttgaa acaatttctc gcgaaacttc
    20401 ttccttgttt acagagaata ttctgacgat ttcaactttc ttacctaatt taccttcttg
    20461 ttttactctt acaattcctt caactaaatc aggattttct tgaatttctt cttgaaaatc
    20521 tatttttatc tctatagttt ccttacgata taagagttca ggtttgttca attgacctga
    20581 taaaacttca tcctgtggat ttaatgtatt taccccagtc ttttcttttg gagaaatctt
    20641 ctcctctttc ttcgtttcta gattcttatg ttcggctaat tgttcttgag aatctgaaga
    20701 ttgtttctct tcttttcttg gattgattaa ttcagtagag aaaggttttt caactacttg
    20761 aacttctgtc ggcttagttg aagaaacagg tgtttgttcc tgaatagctt gtactgttga
    20821 tggatggtct acaaaatttg gtgtaacatt ataatccacc ttttgttgtt ttgtaggagt
    20881 ggctgctgat ttttcttgat tacttacttc aaaatcagaa gtcgtttttc cctctttgat
    20941 atatccaata taagtgtaac ctgaaatctc tttaggaaga ggtaattttt ctccagaggt
    21001 caattcatag tccgtattgt aatttagcaa aagatgattt tctaaagcat ggactgaaac
    21061 taagacacca tttcctattc ccgcaaccaa tactaaatgt aataccgttt tattcttaac
    21121 ctttttcttg gaaacagcaa aaattaaaat tcccagagca gctaagctag caccagcaac
    21181 tagggcttgc ctctcattct tgcttccagt atttggtaat tccgccagtt gattttgaga
    21241 atttaactta taaacaagat aataagtttc atcatcattc tccacgtatg tcggaatatc
    21301 atagacaagc tgcttctttt cttctgatga tagctctgaa tctgccacat atttatagtg
    21361 aactcccgca gtttcttgag catccacaga tgaactagct aatacagaca taaaaaataa
    21421 acttgaaatc gttgcagata caagtcctac tgataatttt ctaaatgaaa aacgctcttg
    21481 tttttcacca aaatactttt ccattattcc tccttgaaat aaaatttata tatgttacaa
    21541 agacctttat tatattagtg tattatctat tatctataga aaaggcagta taccttaatt
    21601 atactcttaa tttacaaaaa agtcttaaaa ttgagatgcg ctttcatact ttgttttata
    21661 ttatttgggg gtacaataac acctaccatg aaatttacac ggtaggtgtt actcatatca
    21721 ctaatcgttc taaaaatggt ttgaggcagt tgaggagaat tccttctatc cagcttcctt
    21781 gtgctgatga gcgatggtct tcctgcaggc ttttttttag aaaatctcgg acttgttctg
    21841 gtgcgatttc aaattcaaag gctttcattt tatagaaaaa gtcgatgaga tgatctgaca
    21901 ggtattcagt tgaaaagggt acttcaccat tttttcgata ttctaataag agcctagaaa
    21961 atcgagcttt ttcttcagga agctcacgaa aataggaatt gaggagccaa gtctgcttct
    22021 gttttctttc aattggatcc tgactggcaa ttcgttggtc tttttccagc tctttttggt
    22081 attgtttggc cttgatagcc cgttctgctc tatttttacc aaaaagaatt ttttcccact
    22141 tgcgttcttc ttgagtcagg gtctctgtaa agccaaagta atcttgataa gcacgctctg
    22201 cgggtcccat ggctagaacc agattgtctg catattgctt ggcgatttta tccctcttct
    22261 tacgttcttt ctctgcctgg atacggagtt cttgttcgta gtcaattttc tccttgccta
    22321 gcttgacaag gtagagttgg tcatccgatt tcccaagtaa aaagggtttg atacactttt
    22381 caaggacttc ttccatccga gcctttttct ttggttccgc cttggtccaa cttcctccct
    22441 gaaagacttc taggaaaagc tggtagtctc tctcaggcgc aaattgattg ccacgattgg
    22501 gtttgaaaac acctttttcc cagagccatt ttagaagtcg ctcgtcaaag tcacttttat
    22561 tgaccttgat tttttccttt ttctgagctt ttctggttag attttcaacc tttctgagca
    22621 gtttttcttc ctcttccaat tgctggtcaa gggacaatcg atgaaaatga cgaacacagt
    22681 cgctaccaat tggaaagagg cgttggcctg tgacaccgtt aaagagttca taagcgtatt
    22741 tgatggcatt tccacagaca caattgctac ggccaatacc gttaaaaatc aaggaaactt
    22801 cattccattc cttggtagct tgttcccaag tatccgcttt cgaagcctgt aaaactgcat
    22861 cgtgcaggga ttttctaact ggaagtgtca tgaggtctcc tttctaatac tcaataaaaa
    22921 tcaaagagca aactagaaag ctagccgcag tcagctcaaa acactgtttt gaggttgtag
    22981 atagaactga cgaagtcagc tcaaaacact gttttgaggt tgtggataga actgacgaag
    23041 tcagtaacca tatatacagc aaggcgaagc tgacgtggtt tgaagagatt ttcgaagagt
    23101 ataagttata cttttacaac ttgaacctcg tctttaccga gtaaaatcaa gtatttttca
    23161 atattttcaa tcgaataggc tcgtgataaa gcctcttcgt atagagctaa ctgaccacga
    23221 tagcggtcta cgagttgact tggttcatca tagcggtctg tcttgtagtc gaacagaaca
    23281 attttgtttt cgtaaagcag atagccatca aggataccac ggacaacaaa gtcttcctga
    23341 ctcttttggt ctcgtttgag catggagaaa ggttgctcgc gatagagatg gtcggtatta
    23401 gcaagaattt cctgaccgag tgctgtgtca aagaaagcaa gaattttatc aagattgatc
    23461 ttgtctctga cagcttggct agtttgaact tgtttgagtg tttctgttag gctagcaagg
    23521 gttagttgct ggctgaggtc aattatctgc atgagttcgt gagtagcact accaatctca
    23581 gctccagtta ccttttcttt ggttgaaaaa tctggcaaat cgaagctgat tttcttgcct
    23641 actgactgac cttgaccagc aatctcgaca ccttccatat ccataactgg ttcgtagaat
    23701 ttcttgattt gacttggggt ttgaacacta ggaagttcaa tagctgcgcg gtgaagagta
    23761 ttataaactt ccacctcctt cagcatttcc agagcttctt tgatggtatc tgactgacga
    23821 ttgtctgctt gggagctatc ttggagagga ctcttggttt ccaactctcc gatagcttct
    23881 ctggtcaact gatcttcgcc aataaaacga taactaaagt tgagcttgtc cttagtaaac
    23941 actttactga taacccaaag ccaatcttgg aaattccgtg cttgcagtct agtattgcta
    24001 ttcagtttcc catttttggc tgctgggtat tccttggatt ccagcttttc acgagaaccc
    24061 ttgccgacaa gatagagctt tttctcagcc cgcgtcatag caacatacag caaacgcatc
    24121 tgctcagaat agcttgctag ctgtaattcc tcttcgttct gcctataggt cagactagga
    24181 atggagagtt tgatggtttt aggatagtgg tcttctactg cccctgtctc catcttggca
    24241 atatatttga caccaagacc attctgacga ctgagaatga cttctgacat agagtcttgc
    24301 ttgttgaaat cttgatccat attgaggata aagacgtaag gaaactccag ccctttactc
    24361 ttgtggatgg tcatgagctc tactgcatct tttggcggtg cgacggccac gcttgccaaa
    24421 tcgtgctggg cttctaagac ttggtcaatc atacgaataa aacgcgacaa acctttgaaa
    24481 ttgctctttt caaattgatc agcacgcagt gctagggcat agagattggc ctgcctagca
    24541 ggaccattcg gcaaagcccc aacatagtca taataaaaac ggtcgttgta aatcttccaa
    24601 atcaagtcat agagagagtg ggttttggca tacaagcgcc aagaagctag gatatccatg
    24661 acttgcttta gtttttcagc tagagctgtg tgaatcaagc ctttttgact acttgccatt
    24721 ttttgtgcat tgaccagttt ctcatagaga ttttcgtgga ctttatcctc tgctttctga
    24781 agggacaaac gtgctagctc atcctcatca aaaccaaaca ttggagactt cataagggca
    24841 accaaggcgt agtcttgcag gggattgtga atgacacgaa gagtgtctag catgacttgc
    24901 acttctaggg attggagata attgttttgc tctccgtcag ttttgacagg aatcccgtac
    24961 tcagacaggg cgaggagaat ctggtcattg cgactgcggc tggaggtcag aagggcaatt
    25021 tccttaaagg caacaccttt ttcttgatga agtttcagaa tctccttgat aactaagcgc
    25081 atttcgcctg ttagtttcgt ttctgtttga ctctcttctt cctcacctgt atcgtccttg
    25141 tcgtagagga gaaattctgc cttgttgtct ggattgggag tcagtttggt attggcaaaa
    25201 acaagctggt gcttgttatc atagttgatt tcgccgacct cttggtccat gagacgttca
    25261 aagacatcat tggttgctga cagcacttct gaactactac ggaaattttc cttgaggaga
    25321 atgagcttgc cttcttgggg attttgcgca tagcgttgga atttctcatt gaaaatctgc
    25381 gggtctgcct gacggaaacg atagatggat tgcttgatat ctcccaccat aaagcgattg
    25441 tggccattag acaacaattc cagcatccgt tcttgaatat ggttggtatc ctgatactca
    25501 tcgaccatga cttcatggaa gcgctcctga taagactcac gaacttgtgg gaaattctct
    25561 aaaatctcaa tggtgtaatg gctgatatca gcgaattcaa aggcattttc ctggcgtttg
    25621 cgctgacgat aagcctccac aaaatgactc ataaaggttt ggaaggtttt agctagttcc
    25681 catgtgtctt gatgataacg ttcttgatag tcgagaatgg ttatctggtc tgataattgt
    25741 cctagtttag caaactgggt ctttctctct tcgttgtagg catcagccag gggcttcaaa
    25801 tcagccttac gactggcatt agtcagggct cgaccgtttt tctccttaga gatggcgaca
    25861 acacgcgcaa gcactgcctg ataagcctga ctatcggact cctgatttag ggagccaatt
    25921 tcatccagaa ttaactgaac attttctaaa taggcagcct ttgcaaactc cttggcatcg
    25981 ttatccagat ggtaacggaa aaagctttcc aaatcccaaa gggcttgttt gatttgctca
    26041 gtcagttttt ctttttcact ggtaaaatca gctttctcaa agcctttgag gaaagattca
    26101 ctcagccact tttgaggatt actggtggat tggaggaagt catagatttt atagacctgc
    26161 tggcgcagac cccgttcgtc cttgccacgc ccagcaaagt ttttcagcaa atgactaaag
    26221 gtctctttct gtttaccttg gtaatgcgct tcaaagacct catgaaagac ttcgttttcg
    26281 agaagaagtt gctcgctttg gttttgtaaa atacggaaat taggtgcaat atcaagcaga
    26341 taaccatgtt tgccaaggaa tttttgtgtg aaagagtcca tggttccgat ggcagcgttt
    26401 ggtaggtctg ccaactggcg tcccaagtgt tgtttgaggt cgacatcatc tgtttcttgg
    26461 attttcttgc tgattttttt ctctaaacgt tctttaagtt catttgcagc cttgacggta
    26521 aaggttgaga taaagagttg agaaatttcg acaccacgcg ccaattggtc cagaatgcgc
    26581 tctgccatga caaaggtctt tccagaacca gccgatgctg agaccaggat attctggcca
    26641 gaagtgtaga tagcttcgat ttgctcggca gttttcttct gttccttgct cgaatttgct
    26701 tctgcttctt gcagtttttg aatctcctcc tcacttaaaa agggaataag cttcatcgat
    26761 tcaactcctc tcttattttt tcaagccaag cttgcttgag tttttctccg accagacgct
    26821 tgccatcagc taggtccaac ttttctagga aacgggcttg gcccagatgg taattggctt
    26881 caaagcctgt aatagcctga tgttgctgga cgtatggggc aatgcttctg ccattttcag
    26941 tataaggatt gatggcgaac cggcctgcta aaatcttctc agcagctttc ttgtaaagat
    27001 aggcattgta gtccagtagg agctgaaatt cctcatctgt cagttgattc gccttgtttt
    27061 tgttataaaa ttcgcctaaa taactgcttt ctttttccaa gaagagccct tggtatttca
    27121 tagatttgct ggcttctacc actgctcctg ccagactttt taccgccatc agagattgga
    27181 caggttcagc catttccaag tacatggcgc cgaaaaaatt ctgctcccct tctcttttta
    27241 gggcagcaag ataggttggt aactgagaat tgagcccatt aaagaaatga ggaaactgga
    27301 actgagttag actcgacttg tagtctacta ctcctatcgc tccatcagct ttcaaacggt
    27361 caatccggtc caccttacct cgtacaaaga cactgcgtcc attgtccaat tgaataaagg
    27421 cttgctcttt cccaccgaaa tttgcctctt ctttaatggt ttcgatgact ggattatggc
    27481 ggagaatgtg accagtcgtc cgtgcaacat caagcaaaac ttctttggta aactgggctt
    27541 ccaaactttc ttgataaata gcttcaaatt cgcgttcttg actagtttct tgaatagctt
    27601 gttctagacg gttgtcaaag gaatcttcat caggcaactg caaggcacgt tcaaaaatgc
    27661 ggtgcaagaa atttccatga ctgcgagcat ccggacgcaa acgtaattcc tcctgcaagc
    27721 ctaacacgta gcggaggaaa taactgtatt ggtttctgta aaactcggtc aaaccggacg
    27781 tagataggta aaattcctgt tcagcaggat agagagcttg taaggtatct ttggtcaact
    27841 gcttactgct tggactggtt ggaagggcag gattttccaa accttgctgg tctagttttt
    27901 tacccatcac acgcgccaga accttgacaa aggtcaaatc ttgctcagta tcactcgtct
    27961 cgccctgctg gtgataggca accagactgg acaaaagact gtgataggat cccatatctt
    28021 ccttagacag ccttttgtga ttcatcctct tctcttttcg actaaatccc aaatggacca
    28081 actcttgaag ataggctgac tccttgcttt cactttcatt aaaaaggctt ggagccgaca
    28141 aaactaattg cttacgggca gaattgacca aagaaagcat agtgtagtga tttttcttga
    28201 gattttcact gctggcaatc agtaattgaa cgccttcttc ggtcgcttgg tttatgtttt
    28261 gtctttcttc atctgtcaaa agactggtat tttgcgcgat ttttggtaaa ttgtcctgag
    28321 ttagcccaat ggcgtagaca aagtcagcag tcaaaggcgc aatcaaatcg taactctgca
    28381 ccagaacggt gtccactgtc gctggaatag tgcgatactg agacaaactc attccagaat
    28441 ggagcaaggc taggaagtcc tctagactaa tctgtgaacc agcaaaaaca gtcgcaaatt
    28501 gttctaaaac atgacagaat gccttccaaa cctcggcttg tctttcctgt tctatagctt
    28561 ccatagtggc tgtcaaatct tgtaactgct tggtcaccac tccttctttt agaaagacat
    28621 tccacttttg caagagattt tcggcctttt gttttcggct agcaaagagg gtttcaagag
    28681 gtgctaaaat tctcagacgg aggacattca aacgctcaag attaaatttt ccatggtggg
    28741 atttggtgaa ggtttgctga aaggctggca agccattgat accaagatag cggatatatt
    28801 gctcaaaagc atcaatatca gactgactga ggtcagtata caaatcagtt ctaagaagat
    28861 tagtcaaatc ctcctgacga aaacggtaac gttttaaagc taaaatagac tcgacaaact
    28921 gagtcaaggg atgatgagcc atggcttcgc ttctaccaag ataaaaagga atctgatact
    28981 ggtcaaaaat ggttttgaga gataactggt aagaagctac atcccccaag agaatacgaa
    29041 aatgcttgta gctcaggtct gagttctcat gtaatttctg acgaatacta cgggctacta
    29101 gctccaactc ctccttttgc gtcaaacaag accagatttg taaattttca cggtctttct
    29161 catcgacatc caaagcgagt tctgaaaagt cataagaaga ctccaacaaa cgagaggcct
    29221 tgtcaaaact atccatcttc tcatgggttt gagaacagtc ctgagcaggc gtttggtatt
    29281 tagaagccag atgatggaga aattttacgc tggcttggta gagattgccc tcgctaaaag
    29341 gactggtata ggctttctta ctagcataag ccccgataac aatctcaaca cctttgccgt
    29401 gaagtaagtc cacaacccgc tcttcctcag cagaaaaacg agtaaagccg tcaatgacca
    29461 aggcgatttg attaaaatca ctacttatct tgtcattctc aatagcctca atcaaatggg
    29521 acaactgact tccctgggct aactgacctt gattaagata ggctgttact ttctcaaaaa
    29581 tcaagagtaa atccgccctc ttatcctcat ctgttaaatt ctccaagtcc aaaaaattca
    29641 tctgagattt ggtcatctca tggtaaagct caattaactg ctggatcaat tgaggatcct
    29701 gcttaatagc gccataaaca cgcaagtcct tgggatcgag ttcggcaagg catttgtaaa
    29761 aggccaaccc aagaccgata tcatcaagag tagttttagc tggtaaatca ttcaagacca
    29821 gataacgagc catttgagca aagcgcgtga cggtaatcga aaaagaagcc tgctgggaca
    29881 agcattccag cacggcgcgt tccttttcaa aagaaagaga gttgggggca atgtagaaga
    29941 cccgcttgcc agctgcaact agctcttctg cctctcttgt tagaatttct gtcaaagaag
    30001 tccgaatatc agtataaagt aatttcatct cagcctcgtt ggaatttttc atcaccctat
    30061 attataccat gattagcctc gtaaatctgt taaaatattt aggccatcct ttcttttctt
    30121 catcatctgc taaatcttaa atacttagct ttacttgtat tagatagaat aagtctggct
    30181 actgaaaatc atataataaa aaagcctcgg taacaaggct ttgagtttta tgattgtttc
    30241 ttaggtacgg aatacacttc aatgtgttgt cccagtgtct taatgtcgac gggtagattg
    30301 tctgatttat cgccatcaac atcggactct aattcgatat cagaagaagt tttaatatca
    30361 cgtgccttta tatattcaat attcttgata gaatgattga actatagtaa attgaaacta
    30421 taatagtaca ccgtggatgc taaaatattg ctagaaatta atttgatttc cctaatcaag
    30481 ctgttcgtat cttatttcaa tcta
//
LOCUS       DAVKWZ010000023        28682 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000023 DAVKWZ010000000
VERSION     DAVKWZ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28682
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.023"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            352..1515
                     /locus_tag="VW854_001054"
     CDS             352..1515
                     /locus_tag="VW854_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEV0262301.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDREVEVQGQEGN
                     FLGTAYLSQQNKGLGWFISKDKVAFNQAFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGLNY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASHTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            1512..2189
                     /locus_tag="VW854_001055"
     CDS             1512..2189
                     /locus_tag="VW854_001055"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEV0262302.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            2179..3033
                     /locus_tag="VW854_001056"
     CDS             2179..3033
                     /locus_tag="VW854_001056"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEV0262303.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLAETVANIRRYQMFGINLSMPYKEQVIPYLDELSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWIGKEMPTEEIW
                     QSLTEKYQ"
     gene            3052..4119
                     /locus_tag="VW854_001057"
     CDS             3052..4119
                     /locus_tag="VW854_001057"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEV0262304.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     IAEEKGLMPAGITQSITEMCQKFGLPVDYENWEVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            4129..5295
                     /gene="aroC"
                     /locus_tag="VW854_001058"
     CDS             4129..5295
                     /gene="aroC"
                     /locus_tag="VW854_001058"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEV0262305.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIENDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLVELDVEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            5305..6408
                     /locus_tag="VW854_001059"
     CDS             5305..6408
                     /locus_tag="VW854_001059"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEV0262306.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEVIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNARDLERAEHLITENTDYTVVIK"
     gene            6419..6757
                     /locus_tag="VW854_001060"
     CDS             6419..6757
                     /locus_tag="VW854_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEV0262307.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            6849..8132
                     /gene="aroA"
                     /locus_tag="VW854_001061"
     CDS             6849..8132
                     /gene="aroA"
                     /locus_tag="VW854_001061"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEV0262308.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKECTRNHTEDMLKQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIVPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSSTLTVESSDLKGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            8125..8601
                     /locus_tag="VW854_001062"
     CDS             8125..8601
                     /locus_tag="VW854_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEV0262309.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     QRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            8598..9446
                     /gene="pheA"
                     /locus_tag="VW854_001063"
     CDS             8598..9446
                     /gene="pheA"
                     /locus_tag="VW854_001063"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV0262310.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            9443..10717
                     /gene="psr"
                     /locus_tag="VW854_001064"
     CDS             9443..10717
                     /gene="psr"
                     /locus_tag="VW854_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEV0262311.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSSPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            10996..11841
                     /locus_tag="VW854_001065"
     CDS             10996..11841
                     /locus_tag="VW854_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV0262312.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLCYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            11831..12934
                     /locus_tag="VW854_001066"
     CDS             11831..12934
                     /locus_tag="VW854_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV0262313.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLARRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYTSKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTNVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            12936..13922
                     /locus_tag="VW854_001067"
     CDS             12936..13922
                     /locus_tag="VW854_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV0262314.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFKAYS
                     QIHRFLKDHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            13938..15371
                     /locus_tag="VW854_001068"
     CDS             13938..15371
                     /locus_tag="VW854_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEV0262315.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGVLARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQAEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRIILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMFTKEN"
     gene            15400..16434
                     /locus_tag="VW854_001069"
     CDS             15400..16434
                     /locus_tag="VW854_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV0262316.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            16634..17371
                     /gene="mecA"
                     /locus_tag="VW854_001070"
     CDS             16634..17371
                     /gene="mecA"
                     /locus_tag="VW854_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEV0262317.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVVFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            17522..18808
                     /locus_tag="VW854_001071"
     CDS             17522..18808
                     /locus_tag="VW854_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEV0262318.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILALDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            18810..19679
                     /locus_tag="VW854_001072"
     CDS             18810..19679
                     /locus_tag="VW854_001072"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEV0262319.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            19755..19832
                     /locus_tag="VW854_001073"
                     /pseudo
     CDS             19755..19832
                     /locus_tag="VW854_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            20019..20957
                     /gene="msrB"
                     /locus_tag="VW854_001074"
     CDS             20019..20957
                     /gene="msrB"
                     /locus_tag="VW854_001074"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV0262320.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLL
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            21015..22739
                     /locus_tag="VW854_001075"
     CDS             21015..22739
                     /locus_tag="VW854_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0262321.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKLELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFVEAPEDIHSELEQKQVTSDRVLQV
                     QELTFTYPDAAQPSLRDISFDMIQGQILGIIGGTGSGKSSLIQLILGLYPVNKGRIDL
                     YLDGLSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGFNQEVTDQELWQALEIAQAK
                     DFVSDKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     ILKAIRENLPNMSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHEKED"
     gene            22741..24489
                     /locus_tag="VW854_001076"
     CDS             22741..24489
                     /locus_tag="VW854_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0262322.1"
                     /translation="MKRQTVNQTLKRLAVDLASHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSLVFWQIFLHMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFVDRQGSGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKELSIDIPAGSKVAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRVSLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            24746..26005
                     /locus_tag="VW854_001077"
     CDS             24746..26005
                     /locus_tag="VW854_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEV0262323.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPIGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    26081..26216
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            26255..26755
                     /locus_tag="VW854_001078"
     CDS             26255..26755
                     /locus_tag="VW854_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEV0262324.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            26831..27199
                     /gene="rplL"
                     /locus_tag="VW854_001079"
     CDS             26831..27199
                     /gene="rplL"
                     /locus_tag="VW854_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEV0262325.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            27446..>28682
                     /locus_tag="VW854_001080"
     CDS             27446..>28682
                     /locus_tag="VW854_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV0262326.1"
                     /translation="MSLRNKIEQHIKELEGGKFQKLGDAYLSRKYNFNIVSLGSQEGT
                     DKTTKGIPDSYAVENGKYVYIMYGTHKSVISKLEGDIQSVKKKILEENIAEDKVGRLI
                     CCHTSSNITIKQKEDLEKMAEPYHLELIGINEIANDLTKIDFQYLAKEYLSISESTEQ
                     VWSINDFIRIHDESKTNAPISNDYIGDVSEIINTIKSSEKRIFLISAKPGTGKTRLAI
                     EICSLLDRNKYNIICVKSNNQDIYQDVKRNLNLHKENIVFIDDVNTTQNYISTLGLLN
                     TTSNIRFILTVRDYAKKDVINNIKVYGYNNIEPELIKDDNFKELLNQFSRNDFTNQEI
                     EHIKTISKSNPRIAVIAAKLSSSQDLTNFNDEIDILKDYYEEILNKNNIIYAEQKTLF
                     ILSYLKKIRLESLEENQEFN"
BASE COUNT         8614 a         5268 c         6392 g         8408 t
ORIGIN      
        1 aaggcttctc tataaaatca aattttccag atacctactg aagctgattt atttcttcat
       61 ctttcttcta ctacctttgt cactaaattt gacagacaca tattgctgat acaacctctc
      121 tacctataat ctcacggtta acctgaggct agtttcatag tttgctattt gattttcgtt
      181 gataaaaaga aatgctcctc caagacggaa gagcattttt tattgtgatt cttacacatt
      241 tatttcctag ttttgcagga agtctagtat accatttttt aaaagaagag taaaaaagca
      301 agggtagttt cttttttgag aattgtctaa aagtttgata taatggtagt tatgaataga
      361 ataagagtca gcaaaagggt tgaaaagaag cttgctaagg ggctagtttt actagaagcc
      421 agtgatcttg agaatgtcaa tcttaaggat cgggaagtag aggtgcaggg tcaggaagga
      481 aactttcttg ggactgccta cctttctcag caaaacaagg gcttgggctg gtttatcagc
      541 aaagacaagg tggccttcaa tcaagctttc tttgaaacgt tgtttagaaa agccaaagaa
      601 aagagaaacg cctactatca agatgatttg acaactgcct ttcgtctctt taatcaagag
      661 ggagatggct ttgggggtct gacagtggac ctttatggcg actacgccgt cttttcttgg
      721 tataactctt atgtttatca gattcgtcag actatatcag aagcctttag acaggttttc
      781 cctgaagttt taggagctta tgagaaaatc cgctttaagg gtttgaacta tgaatctgcc
      841 catgtttatg gtcaagaagc acctgacttt ttcactgttt tagaaaatgg tgtcctgtat
      901 caagtcttta tgaatgatgg cttgatgaca ggaattttcc tagaccagca tgaggttcgc
      961 ggtagtttag ttgacggctt ggctatgggt aaatccttac tcaatatgtt ttcctacaca
     1021 gcggcttttt cagtagctgc ggccatggga ggagctagcc atacaacttc tgttgatcta
     1081 gccaaacgtt cacgagaatt gtctcaagcg cattttcagg caaatgggct cagcacagac
     1141 gagcatcgtt ttatagtcat ggatgtcttt gagtatttca aatatgccaa acgcaaagac
     1201 ttgacctacg atgtgattgt cctagatccg cctagctttg ctcggaataa aaaacaaact
     1261 ttctctgtgg ccaaggatta tcacaagttg atttcccaga gtcttgagat tttaaatccg
     1321 ggagggatta tcattgccag taccaatgct gccaatgttt cccgtcagaa atttacagaa
     1381 caaattgata aaggttttgc aggaagaagt taccagattt taaacaaata tggtcttcca
     1441 gcagattttg cctataataa aaaagatgaa agtagtaatt acctcaaggt gattagtatg
     1501 aaggttagta aatgaaatta atcgtttcag taatgccaag aagtttagag gaggctcagg
     1561 ctctggatgc cacgaggtac ctggatgccg acatcattga atggcgtgcc gactatctgc
     1621 ctaaagaagc gattttgcag gtagctccag ccatttttga aaaattcgca ggccgtgagt
     1681 tggttttcac gctacgaact cgctccgaag gtggagaaat cgacctttct ccagaagagt
     1741 atatccatct aatcaaggaa gttgcgcaac tctatcaacc agactatatt gattttgagt
     1801 actatagcta caaggatgtt tttgaggaaa tgctggactt cccaaatctt gttttgagtt
     1861 accataattt ccaagaaaca cctgagaaca tgatggaaat cttgtcagag ttgacgatcc
     1921 taaatccaaa acttgttaag gttgcggtga tggctcacac ggagcaggat gtcttagact
     1981 tgatgaacta tacacgaggc tttaaaaccc tcaatcctga acaggaatat gtgaccattt
     2041 ctatgggtaa ggtgggcaag gtctctcgta tcactgcgga tgtgactggt tcgagttggt
     2101 cttttgccag tctggatgag gttagtgccc ctgggcagat ttctctagct agcatgaaaa
     2161 aaatcaggga gattttggat gaagcttgat ggctatacac gtttagctgc agttgttgcc
     2221 aatcctatta agcattctat ttctcccttc atccacaata gagcctttga ggcgacagct
     2281 accaacggtg cttatgtggc ttgggagatt gaagcgagtg acttggcaga aacagtggcc
     2341 aatattcgtc gctaccagat gtttggcatc aatctgtcca tgccctataa ggagcaggtg
     2401 attccttatt tggatgagct aagcgatgaa gcgcgcttga ttggtgcggt taatacggtt
     2461 gtcaatgaga atggcaattt aattggatat aatacagatg gcaagggatt ttttaagtgc
     2521 ttgccttctt ttacaatttc aggtaaaaag atgaccctgc tgggtgcagg tggtgcggct
     2581 aaatcaatct tggcacaggc tattttggat ggcgtcagtc agatttcggt ctttgttcgt
     2641 tccgtttcta tggaaaaaac aagaccttac ctagacaagt tacaggagca gacaggcttt
     2701 aaagtggatt tgtgtgcttt agaatatgtt tctgaactgc aagcaaggat tgccgagtcg
     2761 gatttgctag ttaatgccac cagtgtgggc atggatggcc aatcctctcc tgttcctgaa
     2821 aacatagtct taccagaaac tcttttagtt gcagatatca tataccaacc ctttgaaaca
     2881 ccgtttttga aatgggctag aagtcagggc aatccagcag tcaatggtct gggaatgttg
     2941 ctctatcaag ctgcagaagc ttttcaactg tggataggca aggaaatgcc gacagaagag
     3001 atttggcagt ctttaacaga aaaataccaa taaagaaagg gagactctcc tatgaaaatc
     3061 agaatcgata ttcctcatca tccttatgat attcagattg aaaaaggttg tatggcccag
     3121 gctggtcagt ggttgcgaga actctggcaa ccgcaaaagg tagtcattgt gacagataac
     3181 catgtagcct ctctctatgc agagaaggtc aagctcagcc tagaagatgc tggttttcag
     3241 gtagctgttt ttgatttctt agaaggtgaa gaaagaaaga atttaactac tgttcagaaa
     3301 gtctatgaat ttctagtcaa gcaaggtctg actcgtagcg atggaatcgt tgctcttggt
     3361 ggtggcgttg ttggggacct ggctggcttt gtagcctcta cctatatgcg gggtattcac
     3421 tttgttcaga ttccgactag tttgacagct caggttgatt cttctatcgg tggaaagaca
     3481 ggcgttaaca caccatttgc taaaaatatg gtggggacct ttgcccaacc agatggggtt
     3541 ctgattgatc cacttgttct tgaaaccctc ggaaaaagag agttgattga agggatgggt
     3601 gaagttatca agtatggctt gattgaggat ccagaactgt gggctctctt gacgggactg
     3661 aatggttctg ttgagagcat tttggaacat gcagagacct tgattgaaca ttcttgtcag
     3721 gtgaagcgca agatggtggt tgaagatgag ttggacaatg gtattcgtct ttacctcaat
     3781 tttggccaca ctattggcca tgccatcgaa gcgactgccg gttatggcaa ggtcatgcat
     3841 ggagaggctg ttgccatggg aatggtacag atttccaaga ttgctgagga aaaaggcctc
     3901 atgccagctg gcattaccca atctatcaca gagatgtgtc agaaattcgg cttgcctgtt
     3961 gactatgaaa attgggaagt tgacaagctt tatcaggctc ttactcatga caagaaagcg
     4021 cgtggcaaca ccttgaaatt ggtcttggtg ccagagcttg gttcagcgac cattcatcca
     4081 gtttctctgg aagagatgaa agactacttg gtaaaataag gagagtgtat gagatattta
     4141 actgcaggag aatcacacgg cccccgtcta acagctatta ttgagggaat tccagctgga
     4201 cttccattga cagctgagga tatcaatgag gaccttagac gccgtcaggg tggctacggt
     4261 cgtggtggtc gtatgaagat tgagaatgac caggttgtct ttacttcggg cgttcgccac
     4321 gggaagacga caggggcgcc tattactatg gatgtcatca ataaggacca ccagaaatgg
     4381 ctggacatca tgtctgcgga ggacattgaa gaccgcctta aaagcaagcg gaaaattact
     4441 catcctcgcc caggtcatgc cgatttggtt ggggggatta agtaccgttt tgatgatttg
     4501 cgaaattctt tggagcgttc atcagctcgt gaaaccacca tgcgggtggc agttggtgca
     4561 gtagccaaac gcctcttggt tgagctggat gtggagattg ccaaccatgt cgtggtcttt
     4621 ggtggcaagg aaatcgatgt tcctgaaaat ctgacagtcg ctgaaattaa gcaaagagct
     4681 gcccagtctg aagtttctat tgtcaaccaa gaacgagaac aggaaatcaa ggactatatt
     4741 gaccaaatca aacgtgatgg tgataccatc ggtggggttg tggagacagt cgtcggaggc
     4801 gttccagttg gtcttggttc ctatgtccaa tgggatagaa aattggatgc aagattggct
     4861 caagctgttg tctctatcaa tgcctttaaa ggggtggaat ttggtcttgg ctttgaggct
     4921 ggttatcgta aaggcagcca agttatggat gaaattctct ggtctaaaga agacggttat
     4981 actcgccgta ccaataatct aggtggtttt gaaggtggta tgactaatgg gcaacccatc
     5041 gttgttcgtg gggtcatgaa acccattcct actctttata aacctcttat gagtgtggat
     5101 atcgaaaccc acgaacctta caaggcaacc gtggagagaa gtgatccgac tgctcttcca
     5161 gctgcaggaa tggtcatgga agcagttgta gcaacggttc tggcgcaaga aatcctcgaa
     5221 aaattctcat cagataatct tgaggaacta aaagaagcgg tagccaaaca ccgagactat
     5281 acaaagaact attaaggagt tcctatggca aaaacaatct atatcgcagg tcttgggttg
     5341 attggagcct ctatggcact tggtatcaaa cgcgatcatc cagattatga aattttaggt
     5401 tataatcgta gtcaagcttc gagagatatc gccttgaaag aaggcatgat tgaccgtgca
     5461 acggatgatt ttgctagttt tgctcctttg gcagatgtca ttatcctcag cttgccaatc
     5521 aaacaaacta ttgctttcat taaggagttg gccaatttgg atttgcgaga aggcgttatt
     5581 atttcagatg ctggttcgac caagtcaacc attgtggatg cggcggagca gtatttggct
     5641 ggcaagtctg ttcgctttgt cggggcccat cccatggctg gtagtcacaa gacaggggct
     5701 gcttcggcag atgtcaatct ttttgaaaat gcctattata tctttacacc ttcaagcctg
     5761 acaagtcagg acacgcttaa ggaaatgaag gatctgcttt caggtcttca tgctcgtttt
     5821 atcgagattg atgccaagga gcatgatcgt gtcacttctc agattagcca ttttcctcat
     5881 attttggctt ctagtctcat ggagcagact gcggtctatg ctcaagagca tgagatggca
     5941 aggcgctttg cggcaggtgg ttttcgagat atgacccgaa ttgcggaaag cgagccagga
     6001 atgtggacct ccattctctt gtccaatagc gagaccattc tggatagaat tgaggatttc
     6061 aaggaacgtt tggaagtgat tggtcaggcc attagtaagg gagatgaaga gcaaatttgg
     6121 aactttttta accaagcgcg tgaacaacgc cagaccatgg aaatccataa gcgtggtggt
     6181 gtggatagct cttacgacct ctatgttgac gttcctgatg aagaagatgt catcctgcgg
     6241 attttggaac tgctacgtgg aacttccttg gttaacatcc acatcaacga ggaaaatcgt
     6301 gaggatattc acggaatcct acaaatttca tttaaaaatg ctcgagactt ggaaagagct
     6361 gagcatctca taacagaaaa taccgactat acagtcgtca tcaaataagg agaagaacat
     6421 gtcaaatatt tacgatagtg caaacgaact cagtcgcggt ctacgcggat taccagaata
     6481 caaggctgtt aaagcagcta aagatgcgat tgcagcagat gctgaggcaa gcaaaatctt
     6541 tacagaatat cttgcctttc aagaggaaat tcaaaaacta gcacagacag gtcaaatgcc
     6601 agatgcgtcc ttccaagcta agatggaagg ctttggtaaa cagattcaag gaaatagcct
     6661 cttgtcagaa ttctttacca agcaacaaca attggccatt tacctttctg acattgaaaa
     6721 aattgttttt gaaccagttt cagaattgct gaaataagaa aagaacgatc ctaaagtcag
     6781 gaatttttaa ggaatttctg gctttttatg ataaaatagg taaaagtatt gcaagtatga
     6841 ggtccagtat gaaactaaaa acaaacattc gccatttaca tggtagtatc cgcgtcccag
     6901 gtgacaagtc tatcagccac cgttccatta tctttggaag tttggctgag ggtgagacca
     6961 aggtttatga tattctgcga ggtgaagacg ttctttcgac catgcaggtt tttcgtgacc
     7021 ttggtgttga aattgaggat aaagatgggg ttattaccat tcaaggtgta ggcatggctg
     7081 gcttaaaagc gccacaaaat gcccttaata tgggaaattc tggcacctcg attcgcctga
     7141 tttcaggtgt ccttgctggt gcagatttcg aagtagagat gtttggagat gatagtcttt
     7201 ccaaacgtcc tatggaccgt gtgacccttc cactgaaaaa aatgggcgtc agcatttcag
     7261 ggcaaactga acgagacttg cctccccttc gcttaaaagg gacgaaaaac ctaagaccta
     7321 ttcattatga gttgccaatt gcctctgccc aagtcaagtc agccttgatg tttgcagcct
     7381 tacaagctaa gggggagtca gttattatcg aaaaagagtg cacccgtaat catactgaag
     7441 atatgttgaa acaatttggt ggtcatttaa gtgtggatgg taagaaaatc acagtccaag
     7501 ggccacaaaa attgacagga cagaaggtgg tcgtaccagg agatatttcc agtgcagcct
     7561 tttggttagt cgcaggtttg attgttccaa attctcgtct agtgctgcag aatgtgggga
     7621 tcaacgaaac tcgcaccggt attattgatg tcattcgtgc catgggtgga aaattggaaa
     7681 taactgaaat cgatccagtc gctaaatctt ctaccttgac tgttgagtct tctgacttga
     7741 aaggaacaga gattggtggc gctttgattc cacgattgat tgatgaattg cccattattg
     7801 ccctgcttgc gactcaagcc caaggtgtaa cagttatcaa ggatgctgag gagctcaagg
     7861 tcaaggaaac agaccgtatt caggttgtgg cagacgcctt aaatagtatg ggagcagata
     7921 ttactcctac ggcagatggg atgattatca aagggaaatc agctcttcac ggtgctagag
     7981 tcaatacgtt tggtgaccac cgtatcggca tgatgacagc tatcgcagcc ctcttggttg
     8041 cagatggaga ggtggagctt gaccgtgcag aagccatcaa taccagctat cctagtttct
     8101 ttgatgattt ggagagcttg attcatggct aaggtgttat tagggtttat gggggctgga
     8161 aaatcgacta ttgcaagagg cttggaccct aattaccttg atatggatgc tctgattgag
     8221 aagcgcctag gtatgtccat tgcgaatttt ttcgctgaaa agggagaagc agcctttcgt
     8281 caggtagaat cagaagtcct agctgattta ctacaaacag accaagtcgt gtcaactgga
     8341 ggaggagtgg ttatttctca gagaaatcgt gacttactca agactaatac agataacatc
     8401 tacctgaaag cagattttga aaccctctac caacgtatcg cagctgataa ggacaatcag
     8461 cgaccgcttt ttctaaataa tagcaaggaa gaactagtag ctatttttca agaaagacag
     8521 gcttggtatg aggaagtggc tagtcgggtt ttggatgtga ccaagctaag cccagaggaa
     8581 attatagagg aactaagatg aaaattgctt atctaggtcc caaaggatca ttttcacacc
     8641 acgttgtgca gacagctttt cctcatgagg aattgcaggc ttttgccaac attacagatg
     8701 tcatcaaggc ttatgagcaa ggattagtgg actattctgt ggtgccagtt gaaaattcta
     8761 ttgagggtag tgttcatgaa accttggact atctttttca tcaggctcac atccaagcag
     8821 tagcagaaat cgttcagcct attcatcagc agttgatggt ggttccaggc catactaaga
     8881 ttgaaaagat tttttcacat ccacaggcct tggctcaagg aaagaaattc atcgatgaac
     8941 aatatccaga ggctcaaatc gaggtaacag ctagtacagc ttatgcggcc cgttttattt
     9001 ccgaacatcc agaccagccc tttgcagcag ttgcacctag aagttctgct gaagaatatg
     9061 gattggaact gattgccgag gatattcagg aaatggaagc caatttcaca cgtttctggg
     9121 ttctaggagc tgaaaagcct agtattccct tgcaagcaca aactgaaaag atgagtttgg
     9181 ccttgacatt acctgacaac cttccaggtg cactttataa ggccctgtcg acctttgctt
     9241 ggcgaggaat tgacttgaca aaaattgaaa gtcgtccact caagacagca ctgggtgaat
     9301 actttttcat tatcgatgtg gattataccg ataaggactt ggtccacttt gcccaaaaag
     9361 aattagaagc gattggaatc cagtataaaa ttctgggtgc ctatcctatt tatccaatat
     9421 cagaccatgg aaaggagaga agatgagtaa agaaaatccc ttaagtcatc atgagcagtt
     9481 gcgttatgat tatttgctaa aaaatattca ctatctcaat gagagagaaa aaaatgagtt
     9541 tgcctatttg caagaaaagc taactcttgc taggggaaat agtagctcta gcttggaaca
     9601 agaaagagaa gagcaggttg acttaccaag ctatgcgaac cggagtcgct cacaatccaa
     9661 atcacaagca ctctcttccc ctccaaaaaa gaaaagacgg aagctccgtc ttaaacgaat
     9721 ttttatggtg attttttcac ttttggtctg tgtggctttg gccatggtat tcatgttttt
     9781 gcgtggttac caagatgcta gcgcaaagaa aactgctgat gcccgggcag ctcaagtaga
     9841 agtctttaat ggtcaggaca ctagagatgg agttaatatt ttaatcatgg gtactgatgg
     9901 tcgaatcggc cagaacagtg ttgagacacg aactgactct attatggtat taaatgtcgg
     9961 gggctcagat aagaaaatga agctagtcag tttcatgcgt gataatttgg tctatataga
    10021 tggttatagt caagtgatta acggtagaaa acagacagat aacaagttaa acgtagccta
    10081 cgagttagga gaacaagagg ggcaaaaagg ggcagaaatg gttcgccaag tcttgaaaga
    10141 taattttgac ttggacatta agtactacgc cttggttgat ttccaggcct ttgccacagc
    10201 gattgacacg cttttccctg atggggtgac gattgatgct caattttcaa cattgaatgg
    10261 gcgtccccta acagaagcta cagtcggaga tgatttatac gctaccgaga ctgagtctcc
    10321 aacccaaacc attaaagtcg gaaaacagca gatgaatggc tcgaccctgc tcaattatgc
    10381 tcgtttccgt gatgatgatg aggcggatta cggccgtaca aaaagacagc aacaagtttt
    10441 aacagcaatt ttagagcaaa ttaaagatcc cactaaactt ttcactggtt ctgaagctct
    10501 tggaaaggtt tttgctatga cctcaaccaa cgtaccctat actttcctcc taacaaacgg
    10561 tttatctgtt ttggatggag caaaaaatgg tattgaaaaa ttgacgattc cagaactggg
    10621 tgactgggta gatgcctatg atgtttatgg aggcttgggc ttgctggttg atcaaaacaa
    10681 atatcaaacc aagctcgctc aaatgggctt gagataagat aatactcaat gaaaatcaaa
    10741 gtgcaaacta ggaagctagc cgcaggctgc taaaaacaca gttttgaggt tgtagataag
    10801 actgacgaag tcagttacct atacctacgg caaggtgaag ctgacgtggt ttgaagagat
    10861 tttcgaagag tataagacag aaaaatcaaa gtttgatagc tatttggata gcagtcaggc
    10921 tttgattttt tacagtctgc aatagatttt caccccttgt ttgtggtata atgaaacgat
    10981 acgatagaaa gaataatgaa caatatgact gatttaaaag caattcaggc tcgtagtctg
    11041 gagatggctg aatattttgt ggccttttgt aaagaacatg atttgctctg ttatctctgt
    11101 ggcggaggcg ctattggtgc ccttcgaaac aagggattta ttccttggga cgacgaccta
    11161 gactttttta tgcctcgtaa agattatgag aaattagcag aattatggcc tcgttatgca
    11221 gatgaacgtt atttcttgtc aaagagtcac aaggattttg ttgatcgtaa tctttttatt
    11281 accattcgtg acaaggaaac cacctgtatc aagccttatc agcaggattt ggatttgcca
    11341 catggtctgg ccttggatgt tttgcctttg gattattatc cgaaaaatcc agctgagcgg
    11401 aaaaaacagg ttcgttgggc cttgatttat tcactctttt gtgcgcaaac tattccagaa
    11461 aagcatggtg ctctcatgaa atggggaagt cgcattttac tgggtttgac tccaaaatct
    11521 ctctgttatc gcatctggaa aaaagctgag aaagaaatga ctaagtatga tttggctgat
    11581 tgtgatggca ttacagaatt atgctcaggt cctggctaca tgagaaacaa gtacccaatc
    11641 acatcttttg aagacaatct tttcttgcca tttgaaggaa cagagatgcc tattccaatc
    11701 ggctatgatg tctatctcag aactgctttt ggggattata tgacgcctcc accagcagac
    11761 aagcaggtac cgcatcatga tgctgtcatc gctgatatgg ataagtctta tacagaatac
    11821 aagggagaat atggtggcta agaaaaaaat cttatttttt atgtggtctt tttctcttgg
    11881 aggtggtgca gagaagattc tatcaaccat tgtttcaaat ctggatccag aaaagtatga
    11941 tattgatatt cttgaaatgg agcactttga caagggatat gaatctgttc caaagcatgt
    12001 acgcatttta aaatcccttc aagattatcg ccaaaccaga tggttacgag cttttttgtg
    12061 gagaatgaga atttattttc caagactggc tcgtcgtttg cttgtaaaag atgattatga
    12121 tgttgaagtt tcttttacca ttatgaatcc accactgttg ttctctaaaa gaagagaagt
    12181 caagaagata tcttggattc atggaagtat tgaagaactt cttaaggata gctctaaaag
    12241 agaatcacat agaagccagt tggatgctgc gaatacaatt gtagggattt caaaaaagac
    12301 cagcaattct atcaaggaag tttatccaga ttatacttct aaattacaga caatctacaa
    12361 tggatatgat tttcagacta ttctagaaaa atctcaagag aagatcgata tcgagattgc
    12421 tcctcaaagt atctgtacta tcggacggat tgaggaaaat aagggttctg accgtgtagt
    12481 ggaagtgata cgattattac accaagaggg aaaaaactat catctctatt ttatcggggc
    12541 tggtgatatg gaagaggaac tgaaaaaacg agtcaaagag tatgggattg aggactatgt
    12601 acatttcctt ggttatcaaa aaaatcctta tcagtatcta tctcagacga aagttctttt
    12661 gtctatgtct aaacaagaag gttttcctgg agtgtatgtg gaggccttga gtctgggact
    12721 cccttttatc tctacgaacg ttggaggggc tgaggaatta tcccaagaag gacgatttgg
    12781 acaaatcatt gagagcaatc aagaggcagc tcaggcgatt actaattaca tgacttctgc
    12841 ctcaaacttt gatgtcgatg aggctagcca attcattcaa caatttacaa ttacaaaaca
    12901 aatcgaacaa gtagaaaaac tattagagga gtagcatgga aactgcatta attagtgtga
    12961 ttgtgccagt ctataatgtg gcgcagtacc tagaaaaatc gatagcttcc attcagaagc
    13021 agacctatca aaatctggaa attattcttg ttgatgatgg tgcaacagat gaaagtggtc
    13081 gcttgtgtga ttcaatcgct gaacaagatg acagggtgtc agtgcttcat aaaaagaacg
    13141 aaggattgtc gcaagcacga aatgatggga tgaagcaggc tcacggggat tatctgattt
    13201 ttattgactc agatgattat atccatccag aaatgattca gagcttatat gagcaattag
    13261 ttcaagaaga tgcggatgtt tcaagctgtg gtgtcatgaa tgtctatgct aatgatgaaa
    13321 gcccacagtc agccaatcag gatgactatt ttgtctgtga ttctcaaaca tttctaaagg
    13381 aatacctcat aggtgaaaaa atacctggga cgatttgcaa taagctaatc aagagacaga
    13441 ttgcaactgc cctatccttt cctaaggggt tgatttacga agatgcctat taccattttg
    13501 atttaatcaa gttggccaag aagtatgtgg ttaatactaa accctattat tactatttcc
    13561 atagagggga tagtattacg accaaaccct atgcagagaa ggatttagcc tatattgata
    13621 tctaccaaaa gttttataat gaagttgtga aaaactatcc tgacttgaaa gaggtcgctt
    13681 ttttcagatt ggcctatgcc cacttcttta ttctggataa gatgttgcta gatgatcagt
    13741 ataaacagtt taaagcctat tctcagattc atcgtttttt aaaagaccat gcctttgcta
    13801 tttctaggaa tccaattttc cgtaagggga gaagaattag tgctttggcc ctattcataa
    13861 atatttcctt atatcgattc ttattactga aaaatattga aaaatctaaa aaattacatt
    13921 agaacggggg tgaccaagtg attgatggga aacgattatt atttagtttg accatagtca
    13981 gttatgcctt gacgctagta agtggaattg tgtatctgtt taataataat aatgttagct
    14041 tactttctac tttattgttc ttgttggtta gtagcttgat tgcttgttgg aatgatatca
    14101 agtattactt aatccatttt attttctatt taaccatttt tgtatttctg gtatcaagac
    14161 cgaccattga ttattttagg gatggtgctt tggataccta tcatccaata gcctatcgtt
    14221 ttgcctttat agttgtcatg gtttcgattc tgggcttgac aacaggaggc gtcctggctc
    14281 gttacttcat agctaggaag aaaataaaag tagcaaatat aggaaattct ctaaaagagg
    14341 tttatatcaa gcggttacgc tttgtatcac taggagtttt tcttctaact tatcctttct
    14401 atttcattcg gttatttgaa cggctcttgt atcgtttgca gacttcctac tatgcctact
    14461 atgcaaattt tgaaagtaaa ctgccttatt ttacctacat tttgtctacc tttacggtct
    14521 atgcaatgtg tatgtatctg gcaaccaagc caaagaaatt gcaggccaca gcagtgcttg
    14581 tctcctttat tgcagctaac actattcatt tggcaattgg gacacgaaat ccctttattt
    14641 taagtatttt atttgctttt gtttattact ttatgcggga gcaagctgaa aaaggaaaat
    14701 ggattgggtt taaagaaaag ttagcgattt ttgtaggttc tcctattctc atgttagcga
    14761 tgggagtact caattatgta cgggataatg tccaagtttc ccatacaggt ttctgggata
    14821 tcttacttga ctttatctat aaacaaggga ctagttttgg tgttctggct cgaggttttc
    14881 tatttaacag tagcctccct taccgagatt tccgtaattt tacttttggt cctgttcttg
    14941 attattttgc aagggggagt ttgggagcca ttttcggagg aaaagccttt gaacatacaa
    15001 ccaatagtgt ggaactagct attgatagta atagttatgc ccacaatcta tcctatcttg
    15061 tcttgaacaa ggaatacttg aaagggcatg gtatcggaag tagttatatc atggagttgt
    15121 ataccgacta tggtatgatt ggagtctttc tgcttagttt cttactcggc gtattattta
    15181 tagccatgct gcaagtagcc tatcgctcaa ggataatcct atttgcttta tccctactca
    15241 tcttgaataa tctattcttt atgccaagaa gcagcttttc agaaagtttc ttcaatttat
    15301 ttacaatgca attctgggga attgttcttg tgattatatt tgtagcaaaa atgtttacaa
    15361 aggaaaacta gtatctacta aacaaaggag aaaaaaatca tgtttgaaca ttattcagta
    15421 gctgatttgt ttgcaaatct ttataaaaag cgaaaagcaa atattttagc tctcatcgct
    15481 ttatttgctc tcattgctgt accatttaca attaaagcag ttaggaataa aaacactgtc
    15541 aaagatacaa caagttattc aacttatctt atctataaaa tcactcctcc aaaagagtcg
    15601 gccaaaacga ttttgaatca tcaaattggt ggttatagtg atttttatgg gaaattgatt
    15661 gatggtaatt tgaatggagc ttatcttttc aatgatgtag aacccagtga gttgaaaaaa
    15721 attgccagtg aattagacac gacagaaaca accttgaaaa attcaacgaa tgactattgg
    15781 tggaaaaaat tgaccgtcta ctatatgatt gacgatgcag gggttggtgt gaaaattttg
    15841 acatcaagta aagatgccaa tgacttgtta gagaaaaaaa ttgatgggtt gattgagaaa
    15901 tttaaacatg cctatgcaaa tgtgaaaatt gaaaaactgg aaaccatcaa ctctaaagaa
    15961 ttgaacgcaa atggtgaaac agcgcttggc ttaaatgtga aaaatctgat tcttcgttta
    16021 gttgttattg gagtggtttg tgtgattttg gttgtgatgg gaaatgtgtt agtttatctc
    16081 tttaatccaa caatcaatag agtaggtgat ttttctcagt atcaaattga ttttgtaaca
    16141 gagattacaa caattgctaa cctagcagat gttttgtcat acaaaaatac tggacaggaa
    16201 ttgaccatcg ttagctcaaa taaagctatc ctagataaat tgaaacagag tcaagaagct
    16261 ttaaaaggaa tgcattttgt agatttacag gatgtatcat ctcttttgga aagagataca
    16321 gtccttcttg ttgaagagta cggagtgact cgttataaga aatttgagca aagtcttcaa
    16381 attctcagaa acttaaatcg ttctatcctt ggtgtagcaa cctttaaatt ataagtattc
    16441 tgatgtataa ggtcttcaaa atcggctaag agctgatttt gaaggctttt tctatctgtc
    16501 tgcagaaatt ttcttttgga gtctcctata gaaagaccct agggaaaatc tatccgtata
    16561 gaggagcctt cttttgataa aatcgtaaaa atctagtata atagatagaa ctgaaagtat
    16621 gaggttacta gcaatgaaaa tgaaacaaat tagtgataca actttaaaaa tcacgatgtc
    16681 tttagaggat ttgatggatc gtggaatgga gattgctgac tttctcgttc ctcaagaaaa
    16741 aacagaagag ttcttttatg ctatcttgga tgagctagag atgcctgata gctttctgga
    16801 tacaggcatg ttgagcttcc gtgtgactcc aaaacctgat aaggtagatg tctttgtgac
    16861 caagtcaaag attgaccaaa atctagattt tgaagactta tcggatttgc cagatatgga
    16921 agaattggct caaatgtctc cagatgaatt tatcaaaacc ctggaaaaaa gcatcgcaga
    16981 caaaaccaag gatgatatcg aagccattca atctcttgag caagttgaag ccaaggaaga
    17041 agagcaagag caggctgaac aagaagctga gagtaagaaa gaaccttaca tctactacat
    17101 cctttctttt gctaagttgg ctgacttggt agtttttgcc aagacagtga cttttgaaat
    17161 ggaaacttct gaactctaca aaatgaacga gcgctattat ttgaccattt tagtggatat
    17221 tgaaaatcat ccaagcccat atccagcttg gctgttggcc cgtatgcgcg agtttgcaga
    17281 cgatagtgat atcagtcgct cagtcttaca agagtatggt caagtcttga tgagtcacga
    17341 tgcagtgctc aatctgcaaa aaatcggcta atcctttctg gaaagctatt ttcagatttt
    17401 aagaagagcg aatcgtctga ttcgtttttt ctttttatac tgaaatagtg atttactata
    17461 ataggaattt tcacaaaatt ctgttataat ggctatatta gaaaatttcg aggagataaa
    17521 gatgacagtt aaaattgctt tactaggatt tggtaccgtt gcaagtggtg tgcctttcct
    17581 cctaaaggaa aatggaggaa aaatcaatca atcagcacat tcagatatca aagttgctaa
    17641 ggtattggtc aaggatgaag atgaaaaaaa tcgcttgctt gcagcaggga atgactttaa
    17701 ctttgtaacc aatgtggatg atattttatc agaccaggat attactatcg tagtggaatt
    17761 gatggggcgt attgagcctg ctaaaacctt tatcactcgt gccttggaag ctggaaaaca
    17821 cgttgttact gctaacaagg accttttagc tgtccatggc gcagaattgc tagaaatcgc
    17881 tcaagctaac aaggtagcac tttactacga agcagcagtt gctggtggga ttccaattct
    17941 tcgtacttta gcaaattcct tggcttctga taaaattacg cgcgtgcttg gagtagtcaa
    18001 cggaacttcc aacttcatgg tgaccaagat ggtggaagaa ggctggtctt acgatgatgc
    18061 tcttgcggaa gcacaacgtc taggatttgc agaaagcgat ccgacgaatg acgtagatgg
    18121 gattgatgca gcctacaaga tggttatttt gagccaattt gcctttggca tgaagattgc
    18181 ctttgatgat gtagcccaca agggaatccg caatatcaca ccagaagacg tagctgtagc
    18241 tcaagagctt ggttacgtag tgaaattggt tggttctatt gaggaaactt cttcaggtat
    18301 tgctgcagaa gtgactccaa ccttcctacc taaagcgcac ccacttgcta gtgtgaatgg
    18361 cgtaatgaac gctgtctttg tagaatctat cggtattggt gagtctatgt actacggacc
    18421 aggtgctggt caaaaaccaa ctgcaacaag tgttgtagct gatattgtcc gtatcgttcg
    18481 tcgtttgaat gatggtacta ttggcaaaga cttcaacgaa tatagccgtg acttggtctt
    18541 ggcaaatcct gaagatgtca aagcaaacta ctatttctca atcttggctc tagactcaaa
    18601 aggtcaggtc ttgaagttgg ctgaaatctt caatgctcaa gatatttcct ttaagcaaat
    18661 ccttcaagat ggcaaagagg gtgacaaggc gcgtgtcgtt atcatcacac acaagattaa
    18721 taaagcccag cttgaaaatg tctcagctga attgaagaag gtttcagaat tcgacctctt
    18781 gaataccttc aaggtgttag gagaataaga tgaagattat tgtacctgca accagtgcca
    18841 atatcgggcc aggttttgac tcggtcggtg tagctgtaac caagtatctt caaattgagg
    18901 tctgcgaaga acgagatgag tggctgattg aacaccagat tggcaaatgg attccacatg
    18961 atgagcgtaa tctcttgctc aaaatcgctt tgcaaattgt accagacttg caaccaagac
    19021 gcttgaaaat gaccagtgat gtccctttgg cgcgcggttt gggttcttcc agctcggtta
    19081 tcgttgctgg gattgaacta gccaaccaac tgggtcaact caacttatca gaccatgaaa
    19141 aattgcagtt agcgaccaag attgaagggc atcctgacaa tgtggctcca gccatttatg
    19201 gtaatctcgt tattgcaagt tctgttgaag ggcaagtctc tgctatcgta gcagactttc
    19261 cagagtgtga ttttctagct tacattccaa actatgaatt acgtactcgc gacagccgta
    19321 gtgtcttgcc taaaaaattg tcttataagg aagctgttgc tgcaagttct atcgccaatg
    19381 tagcggttgc tgccttgttg gcaggagaca tggtgaccgc tgggcaagca atcgagggag
    19441 acctcttcca tgagcgctat cgtcaggatt tggtaagaga atttgcgatg attaagcaag
    19501 tgaccaaaga aaatggggcc tatgcaacct acctttctgg tgctgggccg acagttatgg
    19561 ttctggcttc tcatgacaag atgccaacaa ttaaggcaga attggaaaag caacctttca
    19621 aaggaaaact gcatgacttg agagttgata cccaaggtgt ccgtgtagaa gcaaaataaa
    19681 gaatagaaga taggatgggg aaactcttga ccagagggtt tcatatcctt tttgtgaaaa
    19741 gaagtttata ctcaatgaaa atcaaagagc aaactaggaa gctagccgca ggctgctcaa
    19801 aacagtgttt tgaggttgtg gatagaactg acgaagtcag ctcaagacac tgttttgagg
    19861 ttgcagatag aactgacgaa gtcagctcaa gacagtgttt tgaggttgca gatagaactg
    19921 acgacgtcag taacatatat acggtaaggt gacgctaacg tggtttgaag agattttcga
    19981 agagtattag ttaaaaacgt gataaaggag aaataaagat ggcagaaatt tatctagcag
    20041 gtggttgttt ttggggccta gaggaatatt tttcacgcat ttctggagtg ctagaaacca
    20101 gtgttggcta cgctaatggt caagtcgaaa cgaccaatta ccagttgctc aaggaaacag
    20161 accatgcaga aacggtccaa gtgatttacg atgagaagga agtgtcactc agagagattt
    20221 tactttatta tttccgagtt atcgatcctc tatctatcaa tcaacaaggg aatgaccgtg
    20281 gtcgccaata tcgaactggg atttattatc aggatgaagc agatttgcca gctatctaca
    20341 cagtggtgca ggagcaggaa cgcatgctgg gtcgaaagat tgcagtagaa gtggagcaat
    20401 tacgccacta cattctggct gaagactacc accaagacta tctcaggaag aatccttcag
    20461 gttactgtca tatcgatgtg accgatgctg ataagccatt gattgatgca gcaaactatg
    20521 aaaagcctag tcaagaggtg ttgaaggcca gtctatctga agagtcttat cgtgtcacac
    20581 aagaagctgc tacagaggct ccatttacca atgcctatga ccaaaccttt gaagagggga
    20641 tttatgtaga tattacgaca ggtgagccac tcttttttgc caaggataag tttgcttcag
    20701 gttgtggttg gccaagtttt agccgtccga tttccaaaga gttgattcat tattacaagg
    20761 atctgagcca tggaatggag cgaattgaag ttcgttctcg ttcaggcagt gctcacttgg
    20821 gtcatgtttt cacagatgga ccgcgggagt taggcggcct ccgttactgt atcaattctg
    20881 cttctttacg ctttgtggcc aaggatgaga tggaaaaagc aggatatggc tatctattgc
    20941 cttacttaaa caaataaaac agagagtggg gcttcccact ttcttcattt ctagaatatg
    21001 aatagaaggg atttatgaaa cacctattat cttacttcaa accctacatc aaggaatcaa
    21061 ttttagcccc cttgttcaag ctgttagaag ctgtttttga gctcttggtt cccatggtga
    21121 ttgctgggat tgttgaccaa tctttacctc agggagatca aggtcatctc tggatgcaga
    21181 ttggtctgct ccttatcttt gcagtaattg gcgttttagt ggccttgata gctcaatttt
    21241 actcagcaaa ggcagcagta ggttttgcta aggaattgac aaacgatctt tatcgtcata
    21301 ttctttcctt gccaaaggac agcagagacc gtctgacaac ttctagtttg gtcactcgct
    21361 tgacttcgga tacctaccag attcagactg gtatcaatca attcctgcgt ctctttttac
    21421 gagcgcccat tatcgttttt ggtgccattt ttatggctta tcgaatctca gctgagttga
    21481 ctttctggtt cttagtcatg gttgccattt tgaccattgt cattgtaggg ttatctcgat
    21541 tggtcaatcc tctctacagt agtctcagaa agaaaacgga ccaactggtt caggaaacgc
    21601 gccagcaatt gcaagggatg cgggttattc gtgcttttgg tcaagaaaaa ctagagttac
    21661 agatttttca aacccttaac caagtttatg ctagattaca agaaaagaca ggtttctggt
    21721 ctagtttatt aacacctctg acctatctga ttgtcaatgg aactcttctc gttattatct
    21781 ggcaaggcta tatttcaatt caaggaggag tgctcagtca aggtgctctc attgctctta
    21841 tcaattacct cttacagatt ttggtggaat tggtcaagct agccatgttg ataaattccc
    21901 tcaaccagtc ctatatctca gccaagcgaa tcgaggaagt ctttgttgag gctccagagg
    21961 atatccattc agaattagaa caaaagcaag ttaccagtga tcgggtttta caagtccaag
    22021 aattgacctt tacctatcct gatgcggccc agccttctct gagagacatt tcctttgata
    22081 tgattcaagg acaaatcctt ggtatcattg gggggactgg ttctggtaaa tcaagcttga
    22141 ttcaactcat acttggactt tatccagtaa ataagggaag aattgacctt tatctagatg
    22201 gactgagtcc tcttaatttg gagcaatgga gatcttggat tgcctatgtg ccacaaaagg
    22261 tcgaactctt taagggaact attcgttcca acttgacttt aggtttcaat caagaagtga
    22321 ctgatcaaga actctggcaa gccttggaaa ttgctcaagc taaggatttt gtcagtgata
    22381 aggaaggact cttggatgct ctagttgaag caggggggcg aaatttctca ggtggacaaa
    22441 aacaaaggct gtctatcgca cgagcagtct tgcgccaggc tccgtttctc atcctagatg
    22501 atgcgacctc ggcactggac accattaccg agtccaagat cttgaaagct atccgcgaaa
    22561 atctgccaaa tatgagctta atcttgattt ctcaacggac ttcgacactc cagatggcgg
    22621 accagattct cctcttggaa aaaggtgagt tgctagctgt tggcaagcac gatgacttga
    22681 tgaaatccag ccaagtctat cgtgaaatca atgcatccca acatgaaaag gaggactaga
    22741 atgaaacgac aaactgtaaa ccagacgctc aaacgtttag ccgtagattt agcaagccat
    22801 cctttcctcc ttttcctagc ctttctagga actattgccc aagttggctt atcaatttac
    22861 ctacctattc tgattgggca ggtcattgac caagtcctag tggctggttc atcactagtt
    22921 ttttggcaga tttttctcca tatgctcttg gttgtaatag gaaatactct ggtacaatgg
    22981 gccaatcctc tcctctataa tcgcctaatt ttctcttata ccagagactt gcgagagcga
    23041 atcatccata agctccatcg tttaccgatt gcctttgtag ataggcaagg tagtggagag
    23101 atggttagtc gtgtaaccac ggacatcgaa cagttggcag ctggcttgac catgattttt
    23161 aaccaatttt tcattggtgt tttgatgatt ttggttagta ttctagccat gctccaaatt
    23221 catctcctca tgactctctt agtcttgctg ttgacgccac tgtccatggt gatttcacgc
    23281 tttattgcca agagatccta tcatctcttc cagaagcaaa cagagacgag gggaattcag
    23341 actcagttga ttgaagaatc gcttagccag cagactataa tccagtcctt caatgctcaa
    23401 acagaattta tccaaagatt gcgtgaggct catgacaact actcaggcta ttctcagtca
    23461 gccatctttt attcttcaac ggtcaatcct tcgactcgct ttgtaaatgc actcatttat
    23521 gctcttttag ctggagtagg agcttatcgt atcatgatgg gttcaacctt gaccgtcggt
    23581 cgtttagtga cttttttgaa ctatgttcag caatacacca agccctttaa cgatatttct
    23641 tcagtgctag ctgagttgca aagtgctctg gcttgcgtag agcgtatcta tggagtttta
    23701 gatagccctg aagtggctga aacaggtaag gaagtcttga ccagtgacca agttaaggga
    23761 gctatttcct ttaaacatgt ctcttttggc taccatcctg aaaaaatttt gattaaggag
    23821 ttgtctatcg atattccagc tggtagtaag gtagccatcg ttggtccgac aggtgctgga
    23881 aaatcaactc ttatcaatct ccttatgcgt ttttatccca ttagctcggg agatatcttg
    23941 ctggatgggc aatccattta tgattataca cgagtatcat tgagacagca gtttggtatg
    24001 gtgcttcaag aaacctggct cacacaaggg accattcatg ataatattgc ctttggcaat
    24061 cctgaagcca gtcgagagca agtaattgct gctgccaaag cagctaatgc agactttttc
    24121 atccaacagt tgccacaggg atacgatacc aagttggaaa atgctggaga atctctctct
    24181 gtcggccaag ctcagctctt gaccatagcc cgagtctttc tggctattcc aaagattctt
    24241 atcttagacg aggcaacttc ttccattgat acacggacag aagtgctggt acaggatgcc
    24301 tttgcaaaac tcatgaaggg ccgcacaagt ttcatcattg ctcaccgttt gtcaaccatt
    24361 caggatgcgg atttaattct tgtcttagta gatggtgata ttgttgaata tggtaaccat
    24421 caagaactca tggatagaaa gggtaagtat taccaaatgc aaaaagctgc ggcttttagt
    24481 tctgaataag ccattctctt ttgaaagttt atggacgaaa aaagttgcct tcgagtgact
    24541 tttttgttac aatagctaga aaaattgttc actgtaatac tcaatgaaaa tcaaagagca
    24601 aactaggaag ctagccgtag gttgctcaaa gcacagcttt gaggttgcag atagaactga
    24661 cgaagtcagt aaccacacct acggcaaagt gaatctgaag tggtttgaag agagtacaac
    24721 ttgtctttta gaaaaggagc ctataatgaa agtctttcag catgtaaata tcgtgacttg
    24781 tgatcaagat ttccatgttt atcttgatgg aatcttagca gtcaaggatt ctcaaatcgt
    24841 ctatgtcggt caagataagc cagcgttttt agagcaagct gagcagatta tagactatca
    24901 gggagcttgg attatgcctg gtttggtcaa ttgtcacacc cattctgcaa tgacaggtct
    24961 gagagggatc cgagatgaca gcaatctcca tgaatggctc aatgactata tttggccagc
    25021 agaatctgag tttactcccg acatgactac caatgcggtc aaagaagccc tgacagagat
    25081 gctccagtca ggaacaacaa cctttaacga tatgtataat cccattggtg tggatatcca
    25141 gcaaatttat caggtggtga aaacttccaa gatgcgttgt tatttttctc cgactctctt
    25201 ttcttcagag acagaaacaa ctgctgagac tataagcaga actcgatcca tcatagatga
    25261 aatcttaaaa tataaaaatc caaatttcaa ggttatggta gcacctcatt ctccgtatag
    25321 ctgcagtaga gacttgctgg aagcgagttt ggaaatggca aaagagctaa atattccgct
    25381 ccatgtccat gtggcggaga ccaaggagga gtcaggaatt atcctcaaac gatacggcaa
    25441 acgccccctt gcttttcttg aagaactggg ttatttagat catccgtccg tatttgctca
    25501 cggggtcgaa ttaaacgaga gagaaattga acgcttggca tcttctcaag tggctatcgc
    25561 ccacaatcct atcagtaacc tcaaactggc atcaggaatt gctccaatta tccagctcca
    25621 aaaagcggga gtagcagtcg gaattgcgac tgactcggtt gcttccaata acaatctaga
    25681 tatgtttgag gaaggaagga ctgcagctct tcttcagaag atgaaaagtg gggatgccag
    25741 ccagtttcca atcgaaacag ctctcaaggt actgacaatc gaaggggcta aggtccttgg
    25801 aatggaaaat cagataggaa gtctggaagt cggcaagcaa gcagattttc tggtcattca
    25861 accacaaggg aaaattcatc tccaacctca ggaaaatatg ctgtctcacc tggtttatgc
    25921 agttaaatct agtgatgtag atgatgttta tatcgccgga gaacaggttg ttaagcaagg
    25981 tcaagtcctg acagtagaac tttaaaagaa aaattacgaa aaattttaaa aaaagtttgc
    26041 aaaaatcttg cattcttttt ttgactatgc tatacttata tacggtttaa aaaaactgcc
    26101 taagacagta ggggagctcg actcataaat atcctaccga ggacaaaacg tatcatgtaa
    26161 aaagaagcgt attgtacttt cgtgtctagg tttgggcgcg tttttctttt tgaaaaattc
    26221 cccaagcaaa ataattacgg aggtgaacac actaatgagt gaagcaatta ttgctaaaaa
    26281 agcggaacta gttgacgtag tagctgaaaa aatgaaagct gctgcatcta tcgtcgttgt
    26341 agacgctcgt ggtttgacag ttgagcaaga tacagttctt cgtcgtgagc ttcgtggaag
    26401 cgaagttgag tataaagtta ttaaaaactc aatcttgcgt cgtgcagctg aaaaagctgg
    26461 tcttgaagat cttgcatctg tatttgttgg accatctgca gtagcatttt ctaatgaaga
    26521 tgttatcgca ccagcgaaaa tcttgaacga cttttctaaa aacgctgaag cacttgaaat
    26581 taaaggtggt gcaatcgaag gcgctgtcgc atctaaagaa gagattcttg cacttgcaac
    26641 tcttccaaac cgcgaaggac ttctttctat gctcctttct gtacttcaag cgccagtgcg
    26701 caacgttgct cttgcagtca aagcggttgc agaaagcaaa gaagacgcgg cttaatctta
    26761 agctacacag cgtagcctag ctacgaaaaa aactattata aaatttaaaa cttatttgga
    26821 ggaaataaca atggcattga acattgaaaa cattattgct gaaattaaag aagcttcaat
    26881 ccttgaattg aacgaccttg taaaagctat cgaagaagaa tttggtgtaa ctgcagctgc
    26941 tcctgtagct gttgctgcag ctgatgcagc tgatgctggt gctgctaaag attcattcga
    27001 cgttgaattg acatctgcag gcgacaaaaa agttggcgtt atcaaagttg tacgtgaaat
    27061 cactggtctt ggtcttaaag aagctaaaga acttgttgac ggtgcaccag cacttgttaa
    27121 agaaggcgtt gcaactgcag aagctgaaga aatcaaagct aaattggaag aagctggagc
    27181 ttcagttact cttaaataat agagcaacta cattagtagc ttaaaaacat gattaaaccg
    27241 ctattcttag gagtagcggt ttttcttttt gtttaaaaag gggcaaactt tttatatagt
    27301 tgtttaggaa ataaataatt tttgtataat tggtttacta ctctaaatta acgtagtaca
    27361 agagtttgtt aattaaacta taccttatgg ttttgactag tgtgatataa ttatgttgaa
    27421 atacttatct ggaaaggttt ttataatgag tttaagaaat aaaattgagc aacatattaa
    27481 agagctagag ggagggaagt ttcaaaaact aggtgacgcg tacctaagta gaaaatacaa
    27541 ttttaatatt gtttcattag gctcacagga aggaacagat aaaacaacta aagggatccc
    27601 agattcttac gctgtcgaaa atggtaaata tgtttatatt atgtatggca cccataagtc
    27661 tgtaatatct aaattagaag gagacattca gtcagttaag aaaaaaattt tagaggaaaa
    27721 tattgctgaa gataaagttg ggagacttat ttgttgtcat acttcttcaa atataacaat
    27781 taaacaaaaa gaagatctag aaaaaatggc tgagccttat catctagaat tgattggaat
    27841 taatgaaatt gctaatgatc ttacaaagat agattttcaa tatttagcaa aagagtatct
    27901 atcaatcagt gaatctacag agcaagtttg gtctatcaat gacttcataa gaattcatga
    27961 tgagagtaaa actaatgctc ctatatcaaa tgactatatt ggagatgtaa gtgaaattat
    28021 aaataccatt aaatcaagtg aaaaacgaat cttcttaata tctgctaagc ctggaacagg
    28081 gaaaactaga ttagcaattg aaatttgctc tttgctggat cgaaataaat ataacattat
    28141 ttgtgtcaag agcaataatc aggatattta ccaagatgta aaacgcaatc taaatttaca
    28201 caaagaaaat attgttttta ttgatgatgt taatactaca caaaattata tttctacact
    28261 tgggctactg aacacgacta gtaatattcg gtttattctt acggtacgtg attatgctaa
    28321 aaaagatgta atcaataata ttaaagttta tgggtacaat aatattgagc cagaacttat
    28381 aaaagatgat aattttaagg aattactcaa tcaattttct cgaaacgatt ttaccaatca
    28441 agaaattgaa catataaaaa cgatatctaa aagcaatccg cgtattgcag tcattgcagc
    28501 aaagttatct agctctcagg atttgactaa ttttaatgat gagattgata ttttaaaaga
    28561 ttactatgaa gaaattttaa ataaaaacaa cattatttat gcagaacaaa agactctatt
    28621 tattttatct tacttaaaaa agattagact ggaaagttta gaagagaacc aggaattcaa
    28681 ta
//
LOCUS       DAVKWZ010000024        27244 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000024 DAVKWZ010000000
VERSION     DAVKWZ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27244
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.024"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(530..622)
                     /locus_tag="VW854_001081"
     CDS             complement(530..622)
                     /locus_tag="VW854_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEV0262327.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
     gene            783..1754
                     /gene="ruvB"
                     /locus_tag="VW854_001082"
     CDS             783..1754
                     /gene="ruvB"
                     /locus_tag="VW854_001082"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEV0262328.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            1735..2307
                     /locus_tag="VW854_001083"
     CDS             1735..2307
                     /locus_tag="VW854_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEV0262329.1"
                     /translation="MNTVKNKQEILEAFRENPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDFETDVDVIFFDPDISYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTAFYTSSRDAMSKYPERCTAVGLRLNEESDFELYVPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLFKKNWQEKWKNLIFKNT"
     gene            2538..3296
                     /locus_tag="VW854_001084"
     CDS             2538..3296
                     /locus_tag="VW854_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEV0262330.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALIKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            3305..4108
                     /locus_tag="VW854_001085"
     CDS             3305..4108
                     /locus_tag="VW854_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEV0262331.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLFQIAIGIIAMLAMHE
                     LLKIRGLATMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            4130..5389
                     /gene="rseP"
                     /locus_tag="VW854_001086"
     CDS             4130..5389
                     /gene="rseP"
                     /locus_tag="VW854_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEV0262332.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            5402..7255
                     /locus_tag="VW854_001087"
     CDS             5402..7255
                     /locus_tag="VW854_001087"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEV0262333.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPTLLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            7355..8734
                     /gene="bglA"
                     /locus_tag="VW854_001088"
     CDS             7355..8734
                     /gene="bglA"
                     /locus_tag="VW854_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEV0262334.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTVFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KKLSDNNGF"
     gene            8926..10734
                     /gene="glmS"
                     /locus_tag="VW854_001089"
     CDS             8926..10734
                     /gene="glmS"
                     /locus_tag="VW854_001089"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEV0262335.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLGGAENHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEEGLSVLEAFKKALHIIRGSYAFAL
                     VDSQDPEVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNRRERTSYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDEAGQ
                     VVVDPDIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLSLLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            complement(10872..10949)
                     /locus_tag="VW854_001090"
                     /pseudo
     CDS             complement(10872..10949)
                     /locus_tag="VW854_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            11006..12055
                     /locus_tag="VW854_001091"
     CDS             11006..12055
                     /locus_tag="VW854_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV0262336.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRSEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(12155..15952)
                     /gene="spuA"
                     /locus_tag="VW854_001092"
     CDS             complement(12155..15952)
                     /gene="spuA"
                     /locus_tag="VW854_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEV0262337.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGSVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSK
                     PEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQASSV
                     KEAVRNESVENSSKENIPATPDKQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNK
                     KEN"
     gene            16369..16782
                     /gene="rpsL"
                     /locus_tag="VW854_001093"
     CDS             16369..16782
                     /gene="rpsL"
                     /locus_tag="VW854_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEV0262338.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            16802..17272
                     /gene="rpsG"
                     /locus_tag="VW854_001094"
     CDS             16802..17272
                     /gene="rpsG"
                     /locus_tag="VW854_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEV0262339.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            17697..19778
                     /gene="fusA"
                     /locus_tag="VW854_001095"
     CDS             17697..19778
                     /gene="fusA"
                     /locus_tag="VW854_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEV0262340.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     SKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            19882..24273
                     /locus_tag="VW854_001096"
     CDS             19882..24273
                     /locus_tag="VW854_001096"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEV0262341.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHGLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEAIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYRSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            24375..24638
                     /locus_tag="VW854_001097"
                     /pseudo
     CDS             24375..24638
                     /locus_tag="VW854_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
     gene            24631..24909
                     /locus_tag="VW854_001098"
     CDS             24631..24909
                     /locus_tag="VW854_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEV0262342.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPTRY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            24930..25082
                     /locus_tag="VW854_001099"
     CDS             24930..25082
                     /locus_tag="VW854_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262343.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGLF
                     SALKEK"
     gene            25104..26345
                     /locus_tag="VW854_001100"
     CDS             25104..26345
                     /locus_tag="VW854_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEV0262344.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEGFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            complement(26399..>27244)
                     /locus_tag="VW854_001101"
     CDS             complement(26399..>27244)
                     /locus_tag="VW854_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEV0262345.1"
                     /translation="KSPGFLEQKQKIADTIIEFNEISEYVKDIPNKNQFIVKDITGKH
                     AHLAHFENTSQHDYKRDKHKKTLNQSNVYSTSLQVVRRASEEPIHYLCKYFGITATEE
                     SLKQLEEIGENISRMENTIDNLYQREKQIKTDFNPPKFILKYFSKDLMKELDIHLPDI
                     KVDYTSYIFEYVSAGGNSSQKTTILFNGETVEATAEYIAKKIKSKQTAQAQRSLMTNK
                     LRTQIKERDHYTCQICAASTAEQSLLLLEIDHIVPVSKGGLSTPDNLQTLCWKCNRSK
                     SNKIL"
BASE COUNT         7645 a         5190 c         6357 g         8052 t
ORIGIN      
        1 ttttcttttt cataataatc tcccttaact ccacccaatc aggtggagtt ttttagctct
       61 atttcaggct tttggggact attctaaaaa taatttttcg atatttttcg gtatttttcg
      121 gattttggtc ggggaattgg cggggacttt tttagcgaat ataactaaga aataggtctg
      181 tggtcgcttc agctagttca aactcaattt gattgtaagt gactgttaat tgaagaactg
      241 gaactactgt aataatgatt ttgcttgtct agtagtggcg tattaaatga gaagtgacat
      301 gtaagattgt ctcttcgttt acgaaatcaa aatttctatg aaattgatag aatccctaac
      361 gttctcttaa tcttaaccgt cttgtcgtac agcggttgaa gaaaatgtaa acctttgaca
      421 aaattcgtga actatgggat aataaaaagg aattgagtac tagttctata tcataggcta
      481 gaaaagaccc caagctcacg accaaataag cttgaggtct tttttgacac tatttgtcct
      541 tgtttagcca tttatcgact atacgaagaa cgacaccgac cacaattggt ccgatgatag
      601 ttttgagtac taattccatc atgggctatc tcacctcctt tcgtaggcgg tgtagcagtg
      661 tcgtataata ttataccaca taagtgctaa acttaggagt cacccaatca ggtggctttt
      721 ttgtttgtgg cttggttttt tgatataata gagccatgag tagaatttta gataatgaga
      781 taatggggga tgaggagtta gtagaacgca cgctccgtcc tcagtattta cgtgaatata
      841 tcggacagga taaggtcaag gaccagctac aaatctttat tgaagctgcc aaaatgcggg
      901 atgaagcgct ggatcatgtg ctcttatttg ggcctccagg tctcgggaaa acgaccatgg
      961 cctttgttat tgccaacgaa ctgggagtca atcttaagca gacgtcgggt ccagtcattg
     1021 aaaaagccgg agatctggta gctattttga atgagttaga gcctggggat gtacttttta
     1081 ttgatgagat ccatcgtttg ccaatgtcag tggaagaggt gctttatagt gctatggagg
     1141 acttctacat agatattatg attggggctg gtgagggtag tcgtagtgtt catttggagt
     1201 taccaccttt taccttgatt ggtgcgacga ctcgggctgg tatgctctcc aatccgctac
     1261 gggcacgttt tgggattaca ggccatatgg agtattatgc ccatgctgac ttgacagaaa
     1321 ttgtcgagcg gacggcagat atttttgaga tggaaatcac tcatgaggca gcatctgagt
     1381 tggccctacg tagtcgtggg acccctcgta ttgccaatcg tctcctcaag cgcgtgcgcg
     1441 attttgccca gataatgggg aatggggtaa ttgatgatat tattaccgat aaggctttga
     1501 ctatgctgga tgttgaccat gaaggtttgg actatgtgga tcaaaaaatc cttcgtacca
     1561 tgattgagat gtacagtgga ggacctgttg gtctaggaac tctttctgtg aatatcgccg
     1621 aagagcgtga gacagttgaa gatatgtatg agccttactt gattcaaaaa ggttttatca
     1681 tgcggacacg gtctggacgg gtggcgactg ctaaggcata tgagcactta ggttatgaat
     1741 acagtgaaaa ataagcaaga aatcttagag gcttttagag aaaatccaga tatgatggcc
     1801 attctgacga tcatccgaga ccttggtctg aaagactcgt ggttggcagc aggttctgtc
     1861 agaaatttca tctggaatct cttgtcagac aaatcacctt ttgactttga aacagatgta
     1921 gatgtgattt tctttgatcc ggatatttct tatgaggaaa ccttattact ggagaaaaag
     1981 ctgagagagg attttcctca gtaccagtgg gaattgaaaa atcaggtcta tatgcatcag
     2041 cacagtcctc acactgcttt ctataccagt tctcgtgatg ctatgagtaa gtatccagaa
     2101 cggtgtacgg cagttggact gcgcttgaat gaagaatcag attttgaact ctatgtacct
     2161 tatggtttgg aggatatttt gaattttcaa gttcgtccaa ctcctcattt cttagaaaat
     2221 gaagaccgaa tggaactcta tcaaacacgt ttattcaaga aaaattggca ggagaaatgg
     2281 aaaaatttga tttttaaaaa tacttaagga aactttaagc tagggagtgt acactaagtt
     2341 cataagttaa gaaaacctta acttaaactc ctaaaacttt ttcataataa tctccctata
     2401 aaaataaagt cgcccaatca ggcggcttat ttttttgaaa aatgggcttg gtgcctgaga
     2461 ataaatagct tagtgataga agaaaatggg gaaatatggt ataatgaaac gatagatttt
     2521 tgaataggaa taagatcatg tttggatttt ttaagaaaga taaggctgtg gaagtagagg
     2581 ttccgacaca ggttcctgct catatcggca tcatcatgga tggcaatggc cgttgggcta
     2641 aaaaacgtat gcaaccgcga gtttttggac ataaggcggg catggaagca ttgcaaaccg
     2701 tgaccaaggc agccaacaaa ctgggcgtca aggttattac ggtctatgct ttttctacgg
     2761 aaaactggac ccgtccagat caggaagtca agtttatcat gaacttgcca gtagagtttt
     2821 atgataatta tgtcccggaa ctacatgcga ataatgttaa gattcaaatg attggggaga
     2881 cagaccgcct gcctaagcaa acctttgaag ctttaatcaa ggctgaggaa ttgactaaga
     2941 acaacacagg attgattctt aattttgctc ttaactatgg tggacgtgct gagattacac
     3001 aggcgcttaa gttgatttcc caggatgttt tagatgccaa aatcaaccca ggtgacatca
     3061 cagaggaatt gattggcaac tatctcttta cccagcattt gcctaaggac ttacgagacc
     3121 cagacttgat tatccgtact agtggagaat tgcgtttgag caatttcctt ccatggcagg
     3181 gagcctatag tgagctttat tttacggaca ccttatggcc tgattttgac gaagcagcct
     3241 tgcaggaagc tattcttgcc tataatcgtc gccatcgccg atttggagga gtttaggagg
     3301 aaatatgacc caggatttac agaaaagaac cttgtttgca gggattgccc tggctatttt
     3361 cctaccaatt ttaatgattg ggggcctctt gtttcagata gcaatcggaa tcatagccat
     3421 gctagccatg catgaacttt tgaagataag aggtctagcg accatgacga tggagggcct
     3481 cttgactctc tttgcaacct ttgcattaac cattcccttg gagaattacc tgactttttt
     3541 gccagttgat gggaatgtgg ttgcctatag tgttttgatt tcaatcatgt taggaacgac
     3601 cgtttttagc aagtcttata cgattgagga tgcggttttc cctcttgcta tgagcttcta
     3661 cgtgggcttt ggatttaatg ctttactaga tgctcgtgtt gcaggtttgg acaaggctct
     3721 cttagccttg tgtatcgtct gggcgacaga cagtggtgcc tatcttgttg ggatgaacta
     3781 tgggaaacga aagttagcac caagggtatc gcctaataaa acccttgagg gtgccttggg
     3841 tggtatttta ggagcaattt tagtaaccat tatctttatg atagttgaca gtacagttgc
     3901 tcttccatat ggaatttaca agatgtcagt ctttgctatt ttctttagca ttgctggaca
     3961 atttggtgat ttactagaaa gttcgatcaa acgtcatttt ggtgttaagg attctgggaa
     4021 atttatccct ggacatggtg gtgttttgga tcgtttcgat agtatgttgc ttgtatttcc
     4081 aatcatgcac ttatttggac tcttttaatc aaaagacgga ggaaacgcta tgctcggaat
     4141 tttaaccttt attctggttt tcgggattat tgtagtggtg cacgagttcg ggcacttcta
     4201 ctttgccaag aaatcaggga ttttagtacg tgaatttgcc atcggtatgg gacctaaaat
     4261 ttttgctcac attggcaagg atggaacggc ctataccatt cgaatcttgc ctctgggtgg
     4321 ctatgtccgc atggccggtt ggggtgatga tacaactgaa atcaagacag gaacgcctgt
     4381 tagtttgaca cttgctgatg atggtaaggt taaacgcatc aatctctcag gtaaaaaatt
     4441 ggatcaaaca gccctcccta tgcaggtgac ccagtttgat tttgaagaca agctctttat
     4501 caaaggattg gttctggaag aagaaaaaac atttgcagtg gatcacgatg caacggttgt
     4561 ggaagcagat ggtactgagg ttcggattgc acctttagat gttcaatatc aaaatgcgac
     4621 tatctggggc aaactgatta ccaattttgc aggtcctatg aacaatttta tcttaggtgt
     4681 cgttgttttt tgggttttaa tctttatgca gggtggtgtc agagatgttg ataccaatca
     4741 gttccatatc atgccccaag gtgccttggc caaggtagga gtaccagaaa cggcacaaat
     4801 taccaagatt ggctcacatg aggttagcaa ctgggaaagc ttgatccaag ctgtggaaac
     4861 agaaaccaaa gataagacgg caccgacttt ggatgtgact atttctgaaa aggggagtga
     4921 caaacaagtc actgttacac ccgaagatag tcaaggtcgt taccttctag gtgttcaacc
     4981 gggggttaag tcagattttc tatccatgtt tgtaggtggt tttacaactg ctgctgactc
     5041 agctctccga attctctcag ctctgaaaaa tctgattttc caaccggatt tgaacaagtt
     5101 gggtggacct gttgctatct ttaaggcaag tagtgatgct gctaaaaatg gaattgagaa
     5161 tatcttgtac ttcttggcaa tgatttccat caatattggg atttttaatc ttattccgat
     5221 tccagccttg gatggtggta agattgtgct caatatccta gaagccatcc gccgcaaacc
     5281 attgaaacaa gaaattgaaa cctatgtcac cttggccgga gtggtcatca tggttgtctt
     5341 gatgattgct gtgacttgga atgacattat gcgactcttt tttagataat cgaggaatat
     5401 tatgaaacaa agtaaaatgc ctatcccaac gcttcgcgaa atgccaagcg atgctcaagt
     5461 tatcagccat gctcttatgt tgcgtgctgg ttatgttcgc caagtttcag caggtgttta
     5521 ttcttatcta ccacttgcca accgtgtgat tgaaaaagct aaaaacatca tgcgccaaga
     5581 attcgaaaag attggtgctg ttgagatgtt ggctccaacc cttcttagtg cagaattgtg
     5641 gcgtgaatca ggtcgttacg aaacctatgg tgaagacctt tacaaactaa aaaaccgtga
     5701 aaaatcagac tttatcttag gtccaactca cgaagaaacc tttacagcta ttgtccgtga
     5761 ttctgttaaa tcttacaagc aattgccact caacctttat caaattcagc ccaagtatcg
     5821 tgatgaaaaa cgcccacgta atggacttct tcgtacacgt gagtttatca tgaaggatgc
     5881 ttatagtttc cacgctaact atgatagttt ggatagtgtt tatgatgagt acaaagcagc
     5941 ctatgagcgt attttcactc gtagtggttt agacttcaag gctattattg gtgacggtgg
     6001 agccatgggt ggtaaggata gccaagaatt tatggccatt acatctgctc gtacagacct
     6061 tgaccgctgg gttgtcttgg acaagtcagt tgcctcattt gacgaaattc ctgcagaagt
     6121 gcaagaagaa atcaaggcag aattgctcaa atggatagtc tctggtgaag ataccattgc
     6181 ttactcaagt gagtctagct atgcagctaa cttagaaatg gcaacaaacg agtacaaacc
     6241 aagcaaccgt gttgtcgctg aagaagaagt tactcgtgtt gcaacgccag atgttaaatc
     6301 aattgatgaa gttgcagcct tcctcaatgt tccagaagaa caaacgatta aaaccctctt
     6361 ctacattgca gatggtgagc ttgttgcagc ccttctagtt ggaaatgacc aactcaacga
     6421 agtcaagttg aaaaatcact tgggagcaga tttctttgac gttgctagcg aagaagaagt
     6481 ggcgaatgtt gttcaagcag gatttggttc acttggacca gttggtttgc cagagaatat
     6541 taaaattatt gcagatcgta aggtgcaaga tgttcgcaat gcagttgtcg gtgctaacga
     6601 agatggctac cacttgactg gtgtgaaccc aggccgtgat tttactgcag aatatgtgga
     6661 tatccgtgaa gttcgtgagg gtgaaatttc cccagatgga caaggtgtcc ttaactttgc
     6721 gcgtggtatt gagatcggtc atattttcaa actcggaact cgctactcag caagcatggg
     6781 agcagatgtc ttggatgaaa atggtcgtgc tgtgccaatc atcatgggat gttacggtat
     6841 cggtgtcagc cgtcttcttt cagcagtgat ggagcaacac gctcgcctct ttgttaacaa
     6901 aacgccaaaa ggtgaatacc gttacgcttg gggaatcaat ttccctaaag aattggcacc
     6961 atttgatgtg catttgatta ctgtcaatgt caaggatgaa gaagcgcaag ccttgacaga
     7021 aaaacttgaa gcaagcttga tgggagctgg ttatgaagtc ttgacagatg accgtaacga
     7081 acgtgtcggt gttaaattta gcgatagtga cttgattggt cttccaatcc gtatcactgt
     7141 tggtaaaaaa gcggccgatg gcattgtaga agttaagatc aaggcaactg gtgacaccat
     7201 tgaggttcat gcagataacg tgcttgaaac gcttgaaatc ctaagcaaga aataaaaact
     7261 ataatcagaa gaaaaacaag gaaaaaatgt aactagtttt taccttgttt tttctgtata
     7321 atgggagaaa tgagtagata aagaggtaaa tgttatgcta agatttccaa aggattttgt
     7381 ctggggatcc tctacttctg gaccgcagac agaaggacgt gtagctggtg acggtaaggg
     7441 agacaatctc tgggattact ggttccaagt ggagccaaat cgttactata atgggattgg
     7501 ttcagataag acatcgactt tttatgaaaa ttgggagcgg gatattgagc ttttgttaga
     7561 gactggtcac acagtctttc ggacttctat tcagtggtca cggatttttc cacaaggctg
     7621 tggaaaagtc aaccctcaag gtgtggattt ttatcgtaag gtctttgagg ctattaaggc
     7681 taaaggaatt cgtctgttag tcaatctcta tcattttgat ttaccttttg cccttcaaga
     7741 ggatggtgat ggttgggaaa ataaggcgac agtctcagcc tatgaagact atgctcgttt
     7801 ttgttttgag acttatggag atttagtgga tcaatggatt acctttaacg agcccatcgt
     7861 tcctgtagaa tttggttatt tttatgatgc ccattatcca cataaggtgg atgcagaggc
     7921 agctgttaag gtagcctatc atacacaatt ggccagcagc cgggctgtta aggcttgcca
     7981 tgaacttttg cctgattcca agattgggat tgtcctcaac ttgacaccgg cttatccacg
     8041 tagccagcat cctgctgatg tcaaggcagc tcgtattgcg gccctttttc aggcccaatc
     8101 tttcttagat ccatctgtct tggggactta tccacaggag ttggtagaaa tcttgcatga
     8161 acacggtctt ttacctgatg ctacagagga agagttggaa ctcattcgtg ataatacggt
     8221 ggacttcctt ggtgtgaact actatcaacc tttgcgtgtt atggctcctc gatttgctaa
     8281 gcatccagag agtccactct taccagaaca tttttacgag ccttatgtga tgcctggacg
     8341 taaaatcaat tctcatcgtg gttgggagat ttatgagcaa gggatttatg acatcgccca
     8401 aaatatcaag gaaaattatg gcaatattga gtggatgttg actgagaatg gtatgggtgt
     8461 tgaaggggaa gaaaaattcc gtcaagatgg aatgattcaa gatgattacc gtattgactt
     8521 tgtaaaaggt catcttcgtg aacttcaccg tgccattgaa gatggtgcca actgtaaggg
     8581 ctacttgatt tggaccttta ttgactgctg gtcatggctc aacagctata aaaatcgcta
     8641 tggtttggtc gaattagact tggaaacgca agaacgtcgt ctgaagaaat caggccactg
     8701 gttcaaaaaa ttaagcgata ataatggatt ttaaaaagga ctctgactga ttatcccaat
     8761 cggtcagagt tttttgctta caggagctta atttgaacta taccaatttt tactcttgac
     8821 aagtgaaaga aacaagtata tactgttttt gctgattcta taaaagatga attagactat
     8881 accaatttta aggagaaagc acagcttgcc tgtgtcgtat atactatgtg tggaattgtt
     8941 ggtgttgttg gaaacacaaa tgcaactgat attttgattc aagggcttga aaagcttgaa
     9001 taccgtggct atgattctgc gggaattttt gtcctaggtg gtgctgaaaa tcacctagtc
     9061 aaggcggttg gtcgtattgc agaattgtct gccaagacag ctggtgttga gggaacaact
     9121 ggtatcggac atactcgttg ggctactcac ggaaaaccaa ctgaggacaa tgctcaccca
     9181 caccgctctg agacagaacg ttttgtcttg gttcacaatg gggtgattga aaactacctt
     9241 gaaatcaagg aagaatacct tgcaggtcac cacttcaagg ggcagacaga tactgaaata
     9301 gccgttcatt tgattggaaa atttgcggaa gaagaagggc tctcagttct tgaagccttc
     9361 aaaaaagccc ttcacatcat ccgtggttct tatgcctttg ccttggttga ctcacaagat
     9421 cctgaagtca tctacgtagc taaaaataaa tcaccacttt tgattggtct tggagaaggc
     9481 tataacatgg tctgctcaga cgccatggct atgattcgtg aaactaacca atacatggaa
     9541 atccatgacc aagagttggt aatcgtcaag gctgatagtg tggaagttca agactatgat
     9601 ggaaatcgtc gtgagcgtac tagctacact gctgaacttg acttgtcaga tatcggtaag
     9661 ggaacttacc cttactacat gctcaaggaa attgacgagc aaccaactgt tatgcggaaa
     9721 ctcatccaag cctacacaga tgaggctggt caagttgtcg tagacccaga tatcattaag
     9781 gctgttcaag acgcagaccg catctacatc cttgcagctg gaacatctta ccatgcagga
     9841 tttgcttcta agaaaatgtt ggaagaattg acagatacac cagttgaact tggaatctca
     9901 tctgagtggg gctacggtat gccacttctc agcaagaaac cactcttcat ctttatcagc
     9961 caatctggtg aaacagcgga tagtcgtcaa gttttggtca aggctaatga aatgggaatt
    10021 ccaagcttaa cagtgacaaa cgttccaggt tcaaccctct cacgtgaagc caactatacc
    10081 atgctccttc acgcaggtcc tgaaattgcc gtggcatcaa ctaaagccta tacagcgcaa
    10141 atcgcagccc ttgccttcct tgcaaaagca gtcggagaag caaatggtaa tgctaaagcg
    10201 caagcctttg acctggttca tgaattgtca atcgtagctc agtctatcga atcaactctt
    10261 tcagagaaag aaaccattga agccaaggtt cgtgaacttc ttgaaacaac tcgtaacgcc
    10321 ttttacatcg gacgtggtca agattactac gtagccatgg aagcaagtct caaactcaaa
    10381 gagatttctt atatccagtg tgaaggtttt gcggcaggag aactcaagca cggaaccatt
    10441 gccttgattg aagaaggaac gcctgtcttg tctctcttgt cagatccagt tcttgccaac
    10501 catactcgtg gaaatatcca agaggtcgca gcccgtggtg ccaaagtcct cactatcgca
    10561 gaagagaatg tagccaaaga taccgacgat atcgtcctta cgaccgtaca tccatacctc
    10621 tcaccaattt caatggtcgt accaacgcaa ttagtcgctt actttgcaac cctccaccgt
    10681 ggcctcgatg tggacaaacc acgtaacctt gccaagtcag taacggtaga ataagctaaa
    10741 aaagtctagt tatctaggcc tttttcttgt gagcaaattg gttgcttgta ctcaagattc
    10801 gtgttatact cttcgaaaat ctcttcaaac cacgtcaacg tcgccttgcc gtaggtatgg
    10861 ttactgactt cgtcagttct atccacaacc tcaaaacact gttttgagca gcctgcagct
    10921 agcttcctag tttgctcttt gattttcatt gagtattaga ataatttttc aaattgaagt
    10981 acagagatag ataaggagaa tcacaatggt agaattggga atttcaacat ttggggaaat
    11041 aacggagctt gaagggactg gacaaactta cagtcatgct gaacgcattc gccagttggt
    11101 ggcagagatt gagctggctg acaaggttgg tttggacgta tatgggattg gcgagcacca
    11161 tcgggcggat tttgcagtat cagccccaga gattgtcctg gccgctgggg cagtcaatac
    11221 caagaaaatc cgtttgacca gtgcagtcag cattctgtca agcatggatc cgattcgttt
    11281 gttccaacag tatgccacta tcgatgcttt gtcaaatgga cgatcggaga ttatggctgg
    11341 aaggggctct ttcacggaat ctttccctct gtttggttat gacttgaaag actacgactc
    11401 tctttttgat gagaaattag acttgcttca gttagtcaat gaaaagacca agttagactg
    11461 gcaaggtcga ttgactcaaa cgattgctgg taaagaagtt tatcctcgtc cagttcagga
    11521 caaattgccc ttgtggatag ctacaggtgg tcatgtcgaa tcaacagtga agattgctca
    11581 ggctggtcta ccgattgtat atgctattat tggtggtaat ccacgttatt ttaaaaagtt
    11641 gattcaggct tatcgtgaga ttggaagcga agcgggccat gctgacaaag acttgaaagt
    11701 gggagcccat tcttgggggt ggattgcgga agatggtgag caggcggtga aagattattt
    11761 ccatccgact aagcaagtgg tggatgctat ttccaaagac cgtccgcact ggcaggaatt
    11821 gcgttatgag caatatttgg agcaggtagg gccaaatggc gccatgtttg ttggcaatcc
    11881 agatcaggtg gccgaaaaat tgattcgcat gatagaggat ttaggattgg accgcttcat
    11941 gcttcatcta ccgcttggtt ctatgcctca tgaccaggtt ctgagagcta ttgaactctt
    12001 cggaacacaa gttgcaccca aagtacgagc ttattttgct atgaaagagg cttaataaaa
    12061 aatcctaatc aagtttatta ggacaacaaa tattgtacaa aaattaatcc cccgagctat
    12121 aatgcttggg ggatttttct ataggagggc tagtttagtt ctctttttta ttttttagta
    12181 agaaaccgag accaaggagc gcaaggaggc tgattcctgc aaataggagt ttgttttcgt
    12241 ttttgattcc tgtatttgga agttcagctt gtttatctgg ggttgcaggt atattttcct
    12301 tgctagagtt ttctaccgat tcgtttcgaa ccgcttcttt tacagatgat gcttgtgctt
    12361 cttgtgctgg ttgttcagct tgtgaatcag ctgtagcttg gctaggttta ttttccgcat
    12421 cagctacttt ggcatctggt ttagtagaat caggtctagc ttctggagct gggtcttgat
    12481 gtgcagggtg agaagcttca ttttgatgtt caggcttgga aggggttgag tctggtttct
    12541 cttctgcagt tgactcatgg ctagttccat tttgagagac tcgaagaaca gtagctgtaa
    12601 gggcattcaa tttcaagcct ttttcagtcc attcaagtcc tttcgggttg gcaattccga
    12661 ctgatcctgc ttggttttca tctgccaaaa cttccgcatt tcttagatgt gcaaaggcag
    12721 ttcccaaatt aaattcgcga gctttttcat ccgcattgac aaagactgcg tagatatcgc
    12781 cgtttggagc agtgatttgg tagccaatca ctacatcctc tttttccaca ccattttggc
    12841 ctgggacagt gatgaggtgg acacggtctt tgatatcttg gagactctta agtcggaagg
    12901 catctgtaga ttgacgaagg gcaatcaaac ctttcatata gtcacggctc ttgacatttt
    12961 caggataagc tttaccatct gtagccttag tccagtcaaa cttgttgact gcgtcgctag
    13021 aatcgtaaga atcatggatg aagtaaggat agtcaaatgg gttgccgtcc ttatcacgca
    13081 acaagtgaga tttgtttgga accttatcct ctgctactgg agtcttgtag gctgggtcac
    13141 ggaattgttt agtacgtcca tattcctgac cggagtggat aaatggagtt ccttgagctg
    13201 tcaagaccat gagatttcca agtcgtaaac gacggtggat ttcagcatag ttctcagcct
    13261 tgcttgggtc ttttttgata gactgggcaa tgatgtcaaa gagggtcaag ttatcatggg
    13321 ctgcgatgta ttggatgaca tctccagggc tgtcagcttc aaagttagtt ggttgagcaa
    13381 tgagattttt aaagatggtg ttgacatcac gcttgccacc tgtgataaag gcaggttgac
    13441 cttcgtttgg ataaccagat ttgaggttgt tacggatgtc atctgaaaag acagcgacag
    13501 tatcggtatg tttcatccaa tcttggtcag cagctttagt aggcatgttt tcatcaccgg
    13561 cataggttct ccaaccttca ccaagcatga tgaggtttgg attgagggcg cgtgcagcct
    13621 tgtaagcttc ttcgatagaa gcggcgtcat ggtctcccat catatcgaag cggaagccat
    13681 ccactttgta ggtatcaact aggtatttga tagagtcaac taggagccgt ttggtcatat
    13741 ggtgggttgt ccccaagcgt ccaccaccaa agctagttcg aggtgtgcca tcggcatcca
    13801 taaagtggta gtagtttggc tctaggtctt caaagatatc gactttggct gtgtggttat
    13861 aaacgacatc taggatagct cccataccac gtttgtggat ttcgttgatg aggtttttaa
    13921 attctgcgat tcgtttttct ggattcttag gatcgcttga gtacatacca gtcaaggaga
    13981 agtagttttg agggtcatat ccccagttgt agttgctgtt gcttgaagcg tagtcagaca
    14041 agcgttcatg gtttttcaat tcattgacaa agtagtaaga caagactgga aggagctgga
    14101 tatgggttac acccaagtct ttgagatagt ctagtttttc aatgaaggct tcaaaagtcc
    14161 caaatggttt ggtcaagtct tttgcaatgg caggatctga agtgaagtca cgcacatgag
    14221 cttcgtagat aacggcgtct tcacgagtct tgaaattgtg aatcttacca taagtcaagt
    14281 cttgaggtcc gagtttagct ggatctacaa aggcggcttt agccacttta tgggcatcgt
    14341 caattttggc atcgtcgcta ttccaagcag caagagattt agcgtaagga tcgagtgcaa
    14401 gaacagtttt accttgacgc tcgatttggt attgataata gtagccagtg aaatctgtga
    14461 ttccgagttt gtttgtgctg tctagagttt gtttccaagt tcctctttcc cctttttcga
    14521 gagcgacagt tccaactact ttgtcagggt cattcttgtc gtagacaaca acagaaacct
    14581 tatcagcact tggtgaccaa agggtcaaat caacttgttt tccttcttct tttaggtcag
    14641 ctcccagttt gccatcatag ctgtatgtct catctttcag gcgccagctt gttttggtag
    14701 tgaattggtc ggaattgtag ctaacagtat aaggatgttt tgtgtcagag aaatctccgc
    14761 tgtaggtcac tttcttacca gcttcatcga ttgcaacatc ggtaatagtt accttgtttc
    14821 ctaggtgatt agtgatgttg gagtgtttga ggatatcttc ttttttagca ccgacaagtg
    14881 ttgaaaagct actttcaatg ctagaagtgc ctacgtgttg ggctcctgtc atacggatat
    14941 catggacata gtatggattt gtgtaaatcg attcatcatc gtcttttagg aaaatttggc
    15001 tatgattttt caaatctgtg aacttataat tttctttacg gattttcacg tcgtctcctt
    15061 gtttgctctc atctagtaat aaaaatccaa attctcttgc ggcttcatta agaggaatgt
    15121 cgatatagcg gccatatttg cctgtagccg taaagtctgt tccgtcaggc cattgagcgc
    15181 tacttggatt ttttacatct ccccagtacc agagagattt cttgtcatag ttgccatctg
    15241 tgcggtagta gttgacgcga acagttcctg caggctgtgg ctcgtaagag aaaaccttgt
    15301 aatcttggtc taaccaagcc tcgttcattt ttggaactag tttttctaca gatttatcgc
    15361 cggttagatt ttttccagct gtattgttga tgaggaagct aattttcttg gcttgttctc
    15421 cctttaattt gacatctagg taatagccgt agtcatcttt cttggcatcc ttgaaggaca
    15481 aagctccgtt tggccagttt tcagatggtt tttcaacatc gtcccaagtc catagtcctt
    15541 gagcatcctt gttttcttca ggaagttttt tgacatggat acggaagtag ttatcttcga
    15601 tgggctcttc tgccttagtt tcagttgtta ctggactagt agaagtagtt ggctctgttg
    15661 caggtttttc ttctgttttc tctagggaag cgtttgcatt tctaggtgaa gaaatatcac
    15721 tttcgttctt accagagcca gctgctgtat cagtaagagg ttgggcaagg gtggttgtat
    15781 ttctgttgtt gggttcgtta gtggttggtg tactttcgtc ggatgagata gttggtgtag
    15841 ccatggcaag caagacaagg cttgcgccga taaggacaga accagttcca tttttgaggg
    15901 aacggatgct gtagaccatt tttttctcag tgtgagatgg tgtttttctc ataatgattc
    15961 tccaatcaat aaatttctat atcagtatag catgtagtaa aacaatatgc aagcgttttc
    16021 tttgatttgt gaaaaaaatt cttacttttg agaagttttc tgtactattt tatatatcag
    16081 tataaatgac ggagataaaa atgtcacata agccaagccg tattattagg aagatgatgt
    16141 gttcccagct atttttattc agaaattgat ctctaacatt accaataaaa aagaaaaata
    16201 tcttgacaag caagggaaaa ttttgttaca ataatagacg gtacttttta cttttggtct
    16261 ctcaagagtg tacagggacg tgctgacaaa tgttgcaaaa gtacacacag atgatatctg
    16321 tcaccaagtg tatcatcacc aaaaataaaa aaacacagga gaatgtagat gcctacaatt
    16381 aaccaattgg ttcgcaaacc gcgtaaatca aaagtagaaa aatctaaatc accagctttg
    16441 aacgttggtt acaatagtca taaaaaagtt caaacaaacg tttcttcacc acaaaaacgt
    16501 ggtgttgcaa ctcgtgttgg aacaatgaca cctaaaaaac ctaactcagc ccttcgtaaa
    16561 ttcgctcgtg tacgtttgag caaccttatc gaagttactg cctacatccc aggtatcgga
    16621 cacaacttgc aagagcacag cgtggtgctt cttcgcggtg gacgtgtaaa agaccttcca
    16681 ggggtacgtt accatatcgt ccgtggtgca cttgatactg caggtgttaa cgatcgtaaa
    16741 caaggtcgtt ctaaatacgg tactaaacgt ccaaaagcat aaggaaaggg gataaagaga
    16801 aatgagtcgt aaaaatagag ctccaaaacg tgacgtattg ccagatccgc tatacaattc
    16861 acaactagtt actcgtctta tcaaccgcgt tatgcttgat ggtaaacgtg gtactgctgc
    16921 ttcaatcgtt tacggtgctt ttgagcaaat caaagaagct actggcaacg atgcacttga
    16981 agtatttgaa acagctatgg aaaacatcat gcctgtactt gaagtacgtg cacgtcgtgt
    17041 tggtggttct aactaccaag tcccagttga agttcgtcca gaacgtcgta caacacttgg
    17101 acttcgttgg ttggtaacaa tcgctcgtct tcgtggtgaa cacacaatgc aagaccgtct
    17161 tgcaaaagaa atcttggatg ctgctaacaa cactggtgca gcagttaaga aacgtgaaga
    17221 tactcaccgt atggctgaag ctaaccgtgc attcgcacac ttccgttggt aagataggat
    17281 gcgaaagcgt taagaaagtc ccagagaaaa tagggaatcg aagcaggttg cggttgcaac
    17341 caatgagatt catctttttc tccagacttt tagcttgagc tcaactaaat catgatgcta
    17401 ggaacggtaa ggatgcaagg tgaaaatagg aaactgacgc agtattcgac gaatacaagg
    17461 agttttatct ttttcacgca gcatcccgtt ccagctcaca tcggctaact aactttagcc
    17521 cgggttcaaa ttagctaaat cgattagtat tagctataac tcagcttacc atctcgtaag
    17581 ttgaaaccaa caatagcatg aaaacattga gaacgggtag gtcctgccta tccgttttta
    17641 ttaaaatcgt gttataatag aatagaaatc aaaaataaat aggagaaaca aacctcatgg
    17701 cacgcgaatt ttcacttgaa aaaactcgta atatcggtat catggctcac gtcgatgccg
    17761 gtaaaacaac aactactgag cgtattcttt actacactgg taaaatccac aaaatcggtg
    17821 aaactcacga aggtgcgtca caaatggact ggatggagca agagcaagaa cgtggtatca
    17881 cgatcacatc tgctgcgaca acagctcaat ggaacaacca ccgcgtaaac atcatcgaca
    17941 caccaggaca cgtggacttc acaatcgaag tacaacgttc tcttcgtgta ttggatggtg
    18001 cggttaccgt tcttgactca caatcaggtg ttgagcctca aactgaaaca gtttggcgtc
    18061 aagcaactga gtacggagtt ccacgtatcg tatttgccaa caaaatggac aaaatcggtg
    18121 ctgacttcct ttactctgta agcacacttc acgatcgcct tcaagcaaat gcacacccaa
    18181 tccaattgcc aatcggttct gaagatgact tccgtggtat cattgacttg atcaagatga
    18241 aagctgaaat ctatactaac gaccttggta cggatatcct tgaagaagac atcccagctg
    18301 aataccttga ccaagctcaa gaataccgtg aaaaattgat tgaagcagtt gctgaaactg
    18361 acgaagaatt gatgatgaaa tacctcgaag gtgaagaaat cactaacgaa gaattgaaag
    18421 ctggtatccg taaagcgact atcaacgttg aattcttccc agtattgtgt ggttcagcct
    18481 tcaaaaacaa aggtgttcaa ttgatgcttg atgcggttat cgactacctt ccaagtccac
    18541 ttgacatccc agcaatcaaa ggtattaacc cagatacaga cgctgaagaa actcgtccag
    18601 catctgacga agagccattt gcagctcttg ccttcaagat catgactgac ccattcgtag
    18661 gtcgtttgac attcttccgt gtttactcag gtgttcttca atcaggttca tacgtattga
    18721 atacttctaa aggtaaacgt gaacgtatcg gacgtatcct tcaaatgcac gctaacagcc
    18781 gtcaagaaat cgacactgtt tactcaggtg atatcgctgc tgccgttggt ttgaaagata
    18841 ctacaactgg tgactcattg acagatgaaa aatctaaaat catccttgag tcaatcaacg
    18901 ttccagaacc agttatccaa ttgatggttg agccaaaatc taaagctgac caagacaaga
    18961 tgggtatcgc ccttcaaaaa ttggctgaag aagatccaac attccgcgtt gaaacaaacg
    19021 ttgaaactgg tgaaacagtt atctcaggta tgggtgaact tcaccttgac gtccttgttg
    19081 atcgtatgcg tcgtgagttc aaagttgaag cgaacgtagg tgctcctcaa gtatcttacc
    19141 gtgaaacatt ccgcgcttct acccaagccc gtggattctt caaacgtcag tctggtggta
    19201 aaggtcaatt cggtgatgtg tggattgaat ttactccaaa cgaagaaggt aaaggattcg
    19261 aattcgaaaa cgcaatcgtc ggtggtgtgg ttcctcgtga atttatccca gcggttgaaa
    19321 aaggtttggt agaatctatg gctaacggtg ttcttgcagg ttacccaatg gttgacgtta
    19381 aagctaagct ttatgatggt tcatatcacg atgtcgactc atctgaaact gccttcaaga
    19441 ttgcggcttc actttccctt aaagaagctg ctaaatcagc acaaccagct atccttgaac
    19501 caatgatgct tgtaacaatc actgttccag aagaaaacct tggtgatgtt atgggtcacg
    19561 taactgctcg tcgtggacgt gtagatggta tggaagcaca cggtaacagc caaatcgttc
    19621 gtgcttacgt tccacttgct gaaatgttcg gttacgcaac agttcttcgt tctgcatctc
    19681 aaggacgtgg tacattcatg atggtatttg accactacga agatgtacct aagtcagtac
    19741 aagaagaaat tattaagaaa aataaaggtg aagactaatc cgtcctcact ctagaaggaa
    19801 gtcacttagt ggcttccttt tgtctttaga aaatacctct aaatatggta aaatagtaga
    19861 agaataatgt gaggaaaatg aatgtcaaat agttttgaaa ttttgatgaa tcaattgggg
    19921 atgcctgctg aaatgagaca ggctcctgct ttagcacagg ccaatattga gcgagttgtg
    19981 gttcataaaa ttagtaaggt atgggagttt catttcgtat tttctaatat tttaccgatt
    20041 gaaatctttt tagaattaaa gaaaggtttg agcgaagaat tttctaagac aggcaataaa
    20101 gctgtttttg aaattaaggc tcggtctcaa gaattttcaa atcagctctt gcagtcctac
    20161 tatagggagg ctttctctga aggtccatgt gctagtcaag gttttaagtc cctttatcaa
    20221 aatttgcaag ttcgtgctga gggtaatcag ctatttattg aaggatctga agcgattgat
    20281 aaggaacatt ttaagaagaa tcatcttcct aatttagcca aacaacttga aaagtttggt
    20341 tttccaactt ttaactgtca agtcgagaag aatgatgtct tgacccaaga gcaggaagag
    20401 gcctttcatg ctgaaaatga gcagattgtt caagctgcca atgaggaagc gctccgtgct
    20461 atggaacaac tggagcagat ggcacctcct ccagcggaag agaaaccagt ctttgatttt
    20521 caagcgaaaa aagctgcagc taaacccaag ctggataagg cggagattac tcctatgatc
    20581 gaagtgacga cagaggaaaa tcgtctggta tttgaagggg ttgtttttga tgtggagcaa
    20641 aaagtgacta gaacaggtcg tgttttaatc aactttaaaa tgacggacta tacttcaagt
    20701 ttttctatgc aaaagtgggt taaaaacgag gaagaggccc agaagtttga cctcatcaag
    20761 aagaattctt ggctccgagt tcgagggaat gtggagatga ataacttcac acgcgatttg
    20821 actatgaacg tacaggatct gcaggaagtt gttcactatg agcggaagga tttgatgcca
    20881 gaaggtgagc gtcgggttga gtttcatgct catactaaca tgtcgactat ggatgctttg
    20941 ccagaggtcg aagagattgt tgcaacagct gctaagtggg gacacaaggc ggttgctatc
    21001 acggaccatg ggaatgtcca gtcctttcca catggctata aggcggctaa gaaagcggga
    21061 atccagctga tctatggaat ggaagccaat atcgtggagg accgtgtccc tatcgtctat
    21121 aacgaagtgg agatggactt gtcagaagca acctacgtgg tctttgacgt ggaaacgacg
    21181 ggactttcag ctatctataa tgacttgatt caggttgcgg cctctaagat gtacaagggg
    21241 aatgttattg ctgaatttga tgaatttatc aatcctgggc atcccttgtc agcttttact
    21301 acagagttaa ctggaattac agatgatcat gtcaaaaatg ccaaaccact agaacaagtt
    21361 ttgcaagaat tccaagaatt ttgcaaggat acggtcctag ttgcccacaa tgctaccttt
    21421 gacgttggct ttatgaatgc taattatgag cgtcatggtc ttccaaagat tagtcagcca
    21481 gttattgata cgctggagtt tgctagaaac ctctatcctg agtataaacg ccatggtttg
    21541 gggcctttga ccaagcgttt tggtgtggcc ttggaacatc atcacatggc caactacgat
    21601 gcggaagcga ctggtcgtct gcttttcatc tttatcaaag aggtagcaga aaaacatggt
    21661 gtgaccgatt tagctagact caacattgat ctaatcagtc cagattctta caaaaaagct
    21721 cggatcaagc atgcgaccat ctatgtcaag aatcaggtag gtctaaaaaa tatctttaag
    21781 ctggtttcct tgtctaatac caagtatttt gaaggagtgc cacggattcc gagaacggtt
    21841 ctagatgccc atcgagaggg cttgatttta ggttcagcct gttcagaggg tgaagttttt
    21901 gacgtggtcg tttctcaagg tgtggatgcg gcggttgagg tggccaagta ttatgacttt
    21961 atcgaggtca tgccaccggc tatctatgcg cccttgattg ccaaagagca ggtcaaggat
    22021 atggaggaac tccagaccat tatcaagagt ttgatagagg ttggagatcg ccttggcaag
    22081 cctgttctgg ctacgggaaa tgttcactat atcgaaccgg aagaagagat ttatcgtgaa
    22141 attatcgtcc gtagtttggg acagggtgcg atgattaacc gaactatcgg tcatggtgaa
    22201 catgcccaac cagcaccact tccaaaggct cattttcgaa cgactaatga gatgttggat
    22261 gaatttgcct ttttgggaga ggaactggct cgtaaactgg ttattgaaaa caccaatgcc
    22321 ttggcagaaa tatttgaacc cgttgaagtc gttaagggtg acttgtatac gcctttcatc
    22381 gacaaggctg aagaaacagt tgctgagttg acctataaga aagcttttga gatttatgga
    22441 aatccgctgc cagatattgt tgatttgcgg attgaaaaag aattaacatc catactgggg
    22501 aatggatttg ctgtgattta tctggcatcg cagatgctgg tgcaacgttc taatgaacgg
    22561 ggttatttgg ttggttctcg tgggtctgtc ggatctagtt tcgttgcgac catgattggg
    22621 attacggaag tcaatcctct ctctcctcac tatgtctgtg gtcagtgtca gtacagtgag
    22681 tttatcacag atggttcgta cggttcagga tttgatatgc cccataagga ctgtccaaac
    22741 tgtggtcaca aactcagtaa aaacggacag gatattccgt ttgagacctt ccttggtttt
    22801 gatggggata aggttcctga tattgacttg aacttctcgg gagaagatca gcctagcgcc
    22861 cacttggatg tgcgtgatat ctttggtgaa gaatatgcct tccgtgcggg aacggttggt
    22921 acggtagctg ccaagactgc ctatggattt gtcaagggtt acgagcgaga ttatggcaag
    22981 ttttatcgtg atgcagaagt agaacgcctc gctcaaggag cggcgggtgt caagcggaca
    23041 acaggccaac acccgggggg aatcgttgtt attccgaact acatggatgt ctacgatttt
    23101 acgcctgtcc agtatccagc agatgatgtc acggctgaat ggcagaccac tcactttaac
    23161 ttccacgata tcgatgagaa cgtcctcaaa ctcgatgtac tgggacatga tgatccgacc
    23221 atgattcgaa aacttcagga tttgtctggt attgacccta ataaaattcc tatggatgac
    23281 gaaggcgtga tggcactctt ttctgggact gatgtgctag gggtaacacc tgaacaaatt
    23341 ggaacgccta cgggtatgtt ggggattcca gagtttggaa caaatttcgt acgtggaatg
    23401 gtagacgaaa cccatccgac aacctttgcg gaattgcttc agctgtctgg tctgtcccac
    23461 ggtactgacg tttggttggg gaatgctcag gatctgatta agcaaggaat agcggaccta
    23521 tcgactgtta tcggttgtcg ggacgacatc atggtttacc tcatgcatgc gggtctggaa
    23581 cctaagatgg cctttaccat tatggaacgg gtacgtaagg gtttgtggct aaagatttca
    23641 gaagaggaga gaaatggcta tatcgaagcc atgaaggcta ataaggtgcc agagtggtat
    23701 atcgaatcct gtgggaaaat taagtacatg ttccctaagg cccatgcggc agcctacgtt
    23761 atgatggcct tgcgtgtagc ttacttcaag gttcaccatc ctatttatta ctactgtgct
    23821 tacttctcca ttcgtgctaa ggcttttgat atcaagacca tgggtgcggg cttggaggcc
    23881 atcaagcgca gaatggaaga aatctctgaa aaacggaaga acaatgaagc ctctaatgtg
    23941 gaaatcgatc tctatacaac tcttgagatt gtcaatgaga tgtgggaacg aggtttcaag
    24001 tttggcaaat tagatctcta ccgtagtcag gcgacagagt tcctcatcga cggggatacc
    24061 cttatcccac catttgtagc aatggatggt ctgggagaga acgttgccaa gcaactggtg
    24121 cgggcgcgtg aagagggaga attcctctct aaaacagaac tacgcaagcg tggtggactc
    24181 tcatcaacct tggttgaaaa gatggatgag atgggtattc ttggaaatat gccagaggat
    24241 aaccagttga gtttgtttga tgagttgttt taaaaaattg cttaataatc tattaaaaga
    24301 ggctaacgta tatccaatag atttacatta gctttctttt ttgttaaaat agtctatgga
    24361 aagagggtga gagtatgtca aagatgagta taagcatccg tctggatagt gaggttaagg
    24421 agcaggccca acaggtgttt agtaatctgg gaatggatat gacaacagct attaatattt
    24481 tccttcgtca ggcaattcaa tatcagggat taccttttga tgttagacta gacgaaaatc
    24541 ggaagttgct ccaagcgtta acggatttag actaaaatcg taatatgagc cagtcttttg
    24601 aatcagtctc agatttgatg gaggacttac gtgcttaaga ttcgctatca taaacagttt
    24661 aaaaaagatt ttaagttggc tatgaagcgt ggtttgaagg cagaattatt agaagaagtt
    24721 ttgaattttc tggttcaaga aaaagaacat cctaccagat atcgtgatca ttcattgacg
    24781 gcatccaagc attttcaagg agttcgtgaa tgccataccc agccagattg gcttttggtt
    24841 tataaagtag acaagtcgga attgatttta aatttgctga ggacaggcag tcacagtgat
    24901 ttattttaat ctattttaag ggggttctca tgaaactaag aatatttgcg gaagataagc
    24961 cggctaagaa ggtatttgaa tatcaattag aacttgctga tcgtacaatt cttctatcaa
    25021 cagcactctt gtcaggtgct attgctttag cgggattatt ctctgctttg aaagaaaaat
    25081 aaaaatagaa aagagaaaat tacatggttt taccaaattt taaagaaaat ctagaaaaat
    25141 atgcgaaatt gttggttgcg aacggaatta acgtgcaacc tggtcacact ttggctctct
    25201 ctattgatgt ggagcaacgt gaattggcac atctaatcgt gaaagaagct tatgccttgg
    25261 gtgcgcatga ggtcatcgtt cagtggacag atgatgtgat taaccgtgag aaatttctcc
    25321 atgccccgat ggagcgtttg gacaatgtgc cagaatacaa gattgctgag atgaactatc
    25381 tcttggagaa taaggctagc cgtcttggag ttcgttcatc tgatccaggt gccttgaacg
    25441 gagtggacgc tgacaagctt tcagcttctg ctaaagctat gggacttgcc atgaagccta
    25501 tgcgtatcgc aactcaatct aacaaggtta gctggactgt agcagctgca gcaggacttg
    25561 agtgggctaa gaaagtcttc ccaaatgctg cgagcgacga agaagcagtt gatttccttt
    25621 gggaccaaat tttcaaaact tgccgtgtct acgaagcaga tcctgttaag gcctgggaag
    25681 agcatgcagc tatcttgaag agcaaggccg atatgcttaa taaggagcaa ttttcagccc
    25741 ttcactacac agcgccagga acagatttaa cacttggttt gccaaagaac cacgtttggg
    25801 aatcagctgg tgctgtcaat gcacagggcg aaggattctt gccaaatatg ccgacagaag
    25861 aggtcttcac agcgcctgac ttccgtcgtg cagatggtta tgtcacttct acaaaaccgc
    25921 ttagctacaa cggaaatatc atcgaaggta ttaaggtaac ctttaaggat ggacaaatcg
    25981 tagatatcac tgctgagaag ggtgatcagg ttatgaaaga ccttgtcttt gaaaatgcgg
    26041 gtgcgcgtgc cttgggtgaa tgtgccttgg taccagatcc aagtccaatt tctcagtcag
    26101 gcattacctt ctttaacacc cttttcgatg aaaatgcgtc aaaccacttg gctatcggtg
    26161 cagcctatgc gactagcgtt gttgatggag cggagatgag cgaagaggag cttgaagctg
    26221 cagggcttaa ccgttcagat gttcacgtag actttatgat tggttctaac caaatggata
    26281 tcgatggtat tcgtgaggat ggaacgcgag tacctctttt ccgtaatggg aattgggcaa
    26341 attagcaaaa atatcccaaa agcaaattga attgtaagta tgcttttggg atatttattt
    26401 ataagatttt attagatttt gaacgattac atttccaaca gagagtttga agattgtcag
    26461 gtgtggaaag tcctcctttt gatacaggga caatatggtc tatctcaagt aacaggagag
    26521 attgttcagc tgtagaggca gcacagattt gacaagtata gtggtctctt tcttttattt
    26581 gagttcgaag tttgtttgtc atcagtgatc gttgagcttg agcagtttgt tttgatttta
    26641 ttttctttgc aatatactct gctgttgctt caacagtttc accgttaaac agaattgtcg
    26701 ttttctgaga actattgcct cctgcactca cgtattcaaa aatataagaa gtgtaatcaa
    26761 cctttatgtc tggtaggtgg atatctaact ctttcattaa atctttagaa aagtatttta
    26821 aaataaattt tggaggatta aagtcggttt ttatttgttt ctctcgttga taaagattat
    26881 caattgtgtt ttccattcga gagatattct ctccgatttc ttcaagctgt ttcaagcttt
    26941 cttcggttgc agttattccg aaatacttac agagataatg tataggttct tcagatgcac
    27001 gtcgcacaac ttgtagggaa gttgaataaa cgttactttg atttaaagtt ttcttatgtt
    27061 tatctcgttt atagtcatgt tggctagtat tttcaaagtg agctaaatgt gcatgtttcc
    27121 cagtaatatc ctttacaata aattgatttt tatttgggat atcttttaca tattctgaaa
    27181 tttcattaaa ctcaataata gtatctgcaa ttttttgttt ttgttctaga aaacctggac
    27241 tttt
//
LOCUS       DAVKWZ010000025        26550 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000025 DAVKWZ010000000
VERSION     DAVKWZ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26550
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.025"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(513..1361)
                     /locus_tag="VW854_001102"
     CDS             complement(513..1361)
                     /locus_tag="VW854_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV0262346.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            1619..1897
                     /locus_tag="VW854_001103"
     CDS             1619..1897
                     /locus_tag="VW854_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262347.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKTLASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            1899..3962
                     /locus_tag="VW854_001104"
     CDS             1899..3962
                     /locus_tag="VW854_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV0262348.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWFSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(4040..5296)
                     /locus_tag="VW854_001105"
     CDS             complement(4040..5296)
                     /locus_tag="VW854_001105"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEV0262349.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADEHNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            5438..7903
                     /locus_tag="VW854_001106"
     CDS             5438..7903
                     /locus_tag="VW854_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV0262350.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGAGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMTKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVDDYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            7968..8879
                     /locus_tag="VW854_001107"
     CDS             7968..8879
                     /locus_tag="VW854_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV0262351.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            9109..9807
                     /gene="dapD"
                     /locus_tag="VW854_001108"
     CDS             9109..9807
                     /gene="dapD"
                     /locus_tag="VW854_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEV0262352.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            9875..11005
                     /locus_tag="VW854_001109"
     CDS             9875..11005
                     /locus_tag="VW854_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEV0262353.1"
                     /translation="MLDLIQTRRALHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPNQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            11017..11559
                     /locus_tag="VW854_001110"
     CDS             11017..11559
                     /locus_tag="VW854_001110"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEV0262354.1"
                     /translation="MKSELRKQVLHEMKAISQEQKQFIDQTLTERLLHHPFYQEAKVI
                     ATYLSFPHEFQTQELIEQALKDGKKVLIPKTYPKGRMDFVVYDPQQLVKTSFGLLEPQ
                     GDLEVVDASQIDLIHVPGLAFTTKGYRIGYGGGYYDRYLEHFSGRTLSTIYPCQIQDF
                     IPEFDKDIPVQEVLIDEGNL"
     gene            11543..12220
                     /locus_tag="VW854_001111"
     CDS             11543..12220
                     /locus_tag="VW854_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEV0262355.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            12234..12311
                     /locus_tag="VW854_001112"
                     /pseudo
     CDS             12234..12311
                     /locus_tag="VW854_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(12521..13456)
                     /locus_tag="VW854_001113"
     CDS             complement(12521..13456)
                     /locus_tag="VW854_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262356.1"
                     /translation="MENKFVEFIENNKKVIISSVAVGVVLVLGFGWYSYNQQQAEQQA
                     KIVQLEKDSKSDKEQVDKLFESFDASSDESISKLKELSETSLKTDAGKDYLNNKVKES
                     SKAIVDFHLQKGLAYDVKDSDEKFKDKATLETNVKEITKQIDFIKKVDETFKQENLEE
                     TLKSLNDLVDKYQKQIELLKKEEEKAAEKAKESSSQSNSSSGASNESYNGSSNSNVDY
                     SSSEQTNGYSNNYGGQDYSGSGDSSTNGGSSEQYSSRNSNSGANNGYYKKYKNIDASG
                     HVRDTYHNESNGDVFDENGNYLGNTGGGIAEPPQR"
     gene            complement(13874..14773)
                     /gene="galU"
                     /locus_tag="VW854_001114"
     CDS             complement(13874..14773)
                     /gene="galU"
                     /locus_tag="VW854_001114"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEV0262357.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDETTGMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FEILEKQAPGAGNEIQLTDAIDTLNKTQRVFAREFTGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(14795..15811)
                     /locus_tag="VW854_001115"
     CDS             complement(14795..15811)
                     /locus_tag="VW854_001115"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV0262358.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKNVGAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQKLFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            16023..16403
                     /locus_tag="VW854_001116"
     CDS             16023..16403
                     /locus_tag="VW854_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV0262359.1"
                     /translation="MREFGEKIKRLRLAKKISRSEFCGDESELSIRQLIRIENGESRP
                     TLTKLKYIAERLGVEDYKLMPSYIELDKEYLELKYFLMRTPTYEDETIAQKKESVLIR
                     FLKSIMIGYLRKKDLSSQIIHIWH"
     gene            complement(16577..17002)
                     /locus_tag="VW854_001117"
     CDS             complement(16577..17002)
                     /locus_tag="VW854_001117"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0262360.1"
                     /translation="MTNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKALTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTCLTE"
     gene            complement(17078..17728)
                     /gene="phoU"
                     /locus_tag="VW854_001118"
     CDS             complement(17078..17728)
                     /gene="phoU"
                     /locus_tag="VW854_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV0262361.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(17743..18495)
                     /gene="pstB"
                     /locus_tag="VW854_001119"
     CDS             complement(17743..18495)
                     /gene="pstB"
                     /locus_tag="VW854_001119"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV0262362.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(18497..19312)
                     /gene="pstA"
                     /locus_tag="VW854_001120"
     CDS             complement(18497..19312)
                     /gene="pstA"
                     /locus_tag="VW854_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV0262363.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(19305..20168)
                     /gene="pstC"
                     /locus_tag="VW854_001121"
     CDS             complement(19305..20168)
                     /gene="pstC"
                     /locus_tag="VW854_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV0262364.1"
                     /translation="MTKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRTFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(20286..21161)
                     /locus_tag="VW854_001122"
     CDS             complement(20286..21161)
                     /locus_tag="VW854_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEV0262365.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIESKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(21328..22659)
                     /locus_tag="VW854_001123"
     CDS             complement(21328..22659)
                     /locus_tag="VW854_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEV0262366.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGQGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSALKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(22660..23367)
                     /locus_tag="VW854_001124"
     CDS             complement(22660..23367)
                     /locus_tag="VW854_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0262367.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(23451..23753)
                     /locus_tag="VW854_001125"
     CDS             complement(23451..23753)
                     /locus_tag="VW854_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV0262368.1"
                     /translation="MTADEIWYFHAGSPLTVHMITADGHYETVTLGLNISKGQQLHYC
                     VPKGTIWGSTVDKDDALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTRY"
     gene            complement(23740..23961)
                     /locus_tag="VW854_001126"
     CDS             complement(23740..23961)
                     /locus_tag="VW854_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV0262369.1"
                     /translation="MNKSYFNPLAPTDKEDLIRTLELAPHVEGGYVRQTKKASDTINL
                     AGKGRALYTSIYFLLRRNESFTFSPIDSR"
     gene            23923..24481
                     /locus_tag="VW854_001127"
                     /pseudo
     CDS             23923..24481
                     /locus_tag="VW854_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(24796..26487)
                     /locus_tag="VW854_001128"
     CDS             complement(24796..26487)
                     /locus_tag="VW854_001128"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEV0262370.1"
                     /translation="MNTKELIASELASIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NEDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
BASE COUNT         7708 a         5039 c         5613 g         8190 t
ORIGIN      
        1 ggatttattt tggattttat ttgttatttt gacctgcctc tatattggta accatgattt
       61 gtttactatc aatcatcaag aattctcttt tcgtggtagc gtttggggtc tggtactggc
      121 cttatatcac ttattgttca ttgataagtt tgtaatttcg aatcgaaaat aaagattaga
      181 actatgattg actggctact tttaatattg attttggagg aagattttgt ctctattatt
      241 tattatttta aatttattta ttttgtataa gatctattct ttgaggcggg aaaaatcgaa
      301 atacttgatt tatacggtct atatcatatt tggggtaaat gtagtatatg gtattcaatg
      361 gttattaaaa gaactgattt caactatttc cccttaatgt gtatgaaaac tataacaaaa
      421 cgcataagat taaggttttt aagcacctgt tcttatgcgt ttttgtaata ttgaagattt
      481 tcggtaagtt ttataccttt gggaagtcta gtttagaatg ctttcccaac cagaatctct
      541 gcttcgatgg taatctctgt cagttggctc cagtcaatct tgctctggtc aacgtgaaag
      601 aggagaggag tcatactgag tagggcttgg agttgttctg ctgtgatagt cttagtcaga
      661 gaggcagttt gactagatag gatggtaaag tgttcttgga aatgctcctt gatatcttgg
      721 ttagaatact ccttgtttgt cagctggtcc tgtactcttt gacggatttc tttgaggtga
      781 ttttcagttg ggataacctt tatcaagata ccgtctttgg ataaaacgcg acgaaattct
      841 ccatagttgg caggtgagaa gatatcaagc agaatatcca tgttagcgtc ttttatagga
      901 aggcgtgcca agtcgccaac gaaccaattg actgcccagt tgggttcact tttagcagcg
      961 atttggactg aatctttgga gatgtcaaag gcatagaaag ttttttcaga gtgactttct
     1021 tgtagtttgc gagaatagaa tccttcacca caaccgatat ccaaaattgt tgtggtagtt
     1081 tttgagcttg caagcaagtc agatacagca tctaagatag cttggtaaaa gccggcttct
     1141 aggatttgtt gacggttttg aaaattttcc ttgtcatagt tagcggattg cttgatttga
     1201 ggtgctagat tgacatagcc aaatttcgcc aagtcaaaag aatgacggtt gcagcacttg
     1261 aaattagtct ctaacagagt cagattttct tgacagatag ggcaggcaaa ggcagtcgca
     1321 gaagcaaaac gctgaagttt gggcttgaga tttgtattca tagtttaagt gtatcaaaag
     1381 aggaaaatga aagcaacttt aggaataagt agatgggaga ttcagtagaa ataaaactaa
     1441 tactcttcga aaatcaaatt caaaccacgt caacgtcgcc ttgccgtact caagtacagc
     1501 ctgcggctag tttcctagtt tgctctttga ttttcattga gtataattct ccaatttata
     1561 gaatgaaatt gcttttatat ctaaattgct atataataat gataaggagg aaataagtat
     1621 gttaaaagaa gtattaaccg ttgcaaaagt tgcgaaaaaa tcttcattat ttttgggtgg
     1681 tgtcgcattt ggtacccttg gtttgaaaac cttagcaagt aaggaagcta aaaaaggtta
     1741 ttctaaagct ttggctaagg cttacaagtt gaaagacgag ctagatgcat ctgtttctgt
     1801 tgtgaagcaa catggagacg atgtcttgca agatgccaaa tatttgtacg agcaagagaa
     1861 aaaagaagag caattagata gccttatagg tgaataatat gtcttttaaa gtgctacata
     1921 gaggatacca acatatccga ctatcatctt ctttttcact taccttggat attcaagact
     1981 atcttcgttc cttggcgaga gatgaaaagg ggattgagtc tatccagttt tacatggatc
     2041 aacagcactt tactctacgc ataaaagaag gcttttctgt attagataat gcagaagcct
     2101 ttttaaaaag aattgataaa gggaaagttt ctgagttgat gactcttccc attcgtagag
     2161 aagagagtgc ttattctatt gtttcaggtg cagcggttaa gcgtgtactt tttcgtagtt
     2221 ttgtgccgta tcctattcgc tatatatgga cttgttatca ggctttgggt tatattagag
     2281 aagcctatca aacactagcg cgtaaggaac taacgatgga agtcttggac tgttcggcta
     2341 ttttattgtc tttgtttatg aaccaatcca agacagctag caatatcatg tttatgcttg
     2401 atttggggaa tcatttagat cagtggtcct tgaaaaaaac tgcaacagat ttagaacaga
     2461 gtcttcttgc aaaagagagc gatgtattcc tagtacaggg cgatacggtt gttagtatca
     2521 agagttccga tgttcaaata ggagatgtct tgatcttatc tcaaggaaat gaaattctgt
     2581 ttgatggaca agtagtttca ggtttaggta tggtcaacga aagttccttg acaggagaga
     2641 gttttccagt tgaaaaaaga gagtctgatt tggtttgtgc aaatacagta ttagaaactg
     2701 gagagttacg cattcgtgta acagataatc agatgaacag ccgtatttta cagctgattg
     2761 agttgatgaa gaaatctgaa gaaaacaaga aaacgaaaca acgctatttc atcaagatgg
     2821 cggataaagt cgtcaaatat aatttcttgg gggctgggct gacttaccta ttgacaggtt
     2881 ctttttctaa ggctatttct ttcctattgg tcgatttctc ctgcgctttg aaaatctcta
     2941 ctcctgtagc ttatttgaca gttatcaagg aggggttgaa ccgtgaaatg gtgattaagg
     3001 atggagatgt tctggagaaa tatctggaag ttgatacttt cttgtttgat aagacaggaa
     3061 caatcacaac tagttatcct atagttgaaa aggtgttacc ttttggagac tatagtgagg
     3121 aagatattct cagaatcagt gcctgtcttg aggaacacat ttatcatcct attgctaatg
     3181 ccatcgtcaa gcaagctgag atagagggaa ttgaacatga ggaaatgcat gggaaactcc
     3241 aatatatcgc aagcaagggg atcaaatctc atatagatgg ccaaccagtt cttattggga
     3301 attatgtctt gatgcaggat gagcagattc atatcagttc agaacaaaat gctttaattg
     3361 aagagtacaa gagtcactac aatctcttat tcttggctta tcaaaatgaa ttgattggaa
     3421 tgttctgcat tcatactcct ttgagaaaag aagcaaaaac agccttggat aaacttaagg
     3481 cacaggggaa aaaattgatt ctggcaacag gagacacctt gattaggaca gaggaattag
     3541 tcaaagattt gccctttgat caggtctata cagacttgaa acctgatggg aaatttgagt
     3601 tagtagagaa actgcagaaa gcaggtcaca ctattttgat ggttggagat ggattgaatg
     3661 actcagcggc tttaactcta tcagatatcg gtgtggtgat gaatgagagt gcagatattt
     3721 ctaagcagat gagtgatatc ttattgttag ataatcgttt ggatttcttc caagagttgg
     3781 attcgttatc atcatctttg caaacactca tcaagaaaaa tattcaagat accgttgtcg
     3841 taaatagtag tttgattggc tttggcttat ttaattggtt cagtccttca aatctctcta
     3901 tcttacataa tctaacaacc ttgcgcattg tcttgcgtag cctgtctatt aaaaatagat
     3961 agatgagagt tattaacagc aaaaaggagt tacctttctc gaggttaact cctttgttta
     4021 tagataaatg ttgaatagtt tagtaagtca atacaaagta ttttttcttc ccacgacgga
     4081 taacagtcag ttcattttct aacttatcag cgtcactcaa gacatagtca aggtcttgga
     4141 tgcggtcgcc gtttacgtag atggctccgt tttggacgtc ttcacgggct tggcgttttg
     4201 agttaactat accagatgag acgagcagtt ccacgatatt gttatgttcg tctgcctgta
     4261 cttggtagtt tggcacacca cgaagtcctt gtttgagctc tttgacagaa aggtttttga
     4321 tgtttcctgc aaagagttgc tcagtgatgt taagtgcttc tttgtaggct tcttctccgt
     4381 gaacaagtgt aacaacttca cgagccaaga ctttttgagc caagcgttcg tgtggcgctg
     4441 cttcaaattg tttacgaata tcttcaatct catcaagtga caagaaagta aagattttca
     4501 agaagcgaac agcgtcagcg tccatcacgt tcatccagaa ttggtacatt tcgtatggag
     4561 aagtcttttc gggattgagc cagacggcat ttccttctga tttaccaaat tttttaccag
     4621 ttgcatctgt gattagtgga acagtgataa cgtgaccagt cttgtccgcc ttacgacgaa
     4681 gcaattcggt accagctgtc atatttcccc actggtcaga accaccgatt tgaagagtga
     4741 cattatggtc ttggttaagg acgaagaagt cgtacccttg catgatttgg taagcgaact
     4801 cagtgtaaga aattcctgtt tcgatccgtt ttttaacaga ttccttactc atcatgtagt
     4861 tgaccgtgaa gtattttcca atatcacgga ggaagtcaat gaagctgatg ctgccaaacc
     4921 agtcgtagtt gttgaccatg acagccttgt tttcgccatt ttcaaagtca agaaaacgag
     4981 aaagttgtcc ttggatagac ttgacccagc catctactgt gtcttttgtt tggagactac
     5041 gttcagcatc tttgaaggac ggatctccga tgagacctgt agcaccgcca acgagcgcat
     5101 aaggtttgtg acctgctagt tgcaagcgac gacttgtcaa gattgcgaca aggtggccta
     5161 ggtgaaggct gtcagcagtt ggatcgtagc cagtataata agaaacttga ccttcttcta
     5221 gggctttacg caaagcttct tcatcagtcg tttgaaatat caaaccacgc tcttttagct
     5281 catcaaaaat gtgcatgtgt cttttctcct ttttaaaatt attgtttcta cctattgtat
     5341 cacaaacttg ggcaatagcc tagcagaata gggaagaaag tggctatttt attgaaaatt
     5401 tcccttttgt ggtataatag atagagtgag gaaatccatg caaaatcaat taaatgaatt
     5461 aaaacgaaaa atgctggaat ttttccagca aaaacaaaaa aataaaaaat cagctagacc
     5521 tggcaagaaa ggttcaagta ccaaaaaatc taaaacctta gataagtcag ccattttccc
     5581 agctatttta ctgagtataa aagccttatt taacttactc tttgtactcg gttttctagg
     5641 aggaatgttg ggagctggga ttgctttggg gtacggagtg gccttatttg acaaggttcg
     5701 ggtgcctcag acagaagaat tggtgaatca ggtcaaggac atctcttcta tttcagagat
     5761 tacctattcg gacgggacgg tgattgcttc catagagagt gatttgttgc gcacttctat
     5821 ctcatctgag caaatttcgg aaaatctgaa gaaggctatc attgcgacag aagatgaaca
     5881 ctttaaagaa cataagggtg tagtacccaa ggcggtgatt cgtgcgacct tggggaaatt
     5941 tgtaggtttg ggttcctcta gtgggggttc aaccttgacc cagcaactaa ttaaacagca
     6001 ggtggttggg gatgcgccga ccttggctcg taaggcggca gagattgtgg atgctcttgc
     6061 cttggaacgc gccatgacta aagatgagat tttaacgacc tatctcaatg tggctccctt
     6121 tggccgaaat aataagggac agaatattgc aggggctcgg caagcagctg agggaatttt
     6181 cggtgtagat gccagtcagt tgactgttcc tcaagcagca tttttagcag gacttccaca
     6241 gagtcccatt acttactctc cttatgaaaa tactggggag ttgaagagtg atgaagacct
     6301 agaaattggc ttaagacggg ctaaggcagt tctttacagt atgtatcgta caggtgcatt
     6361 aagcaaagac gagtattctc agtacaagga ttatgacctt aaacaggact ttttaccatc
     6421 gggcacggtt acaggaattt cacgagacta tttatacttt acaactttgg cagaagctca
     6481 agaacgtatg tatgactatc tagctcagag agacaatgtc tccgctaagg agttgaaaaa
     6541 tgaggcaact cagaagtttt atcgagattt ggcagccaag gaaattgaaa atggtggtta
     6601 taagattact actaccatag atcagaaaat tcattctgcc atgcaaagtg cggttgatga
     6661 ttatggctat cttttagacg atggaacagg tcgtgtagaa gtagggaatg tcttgatgga
     6721 caaccaaaca ggtgctattc taggctttgt aggtggtcgt aattatcaag aaaatcaaaa
     6781 taatcatgcc tttgatacca aacgttcgcc agcttctact accaagccct tgctggccta
     6841 cggtattgct attgaccagg gcttgatggg aagtgaaacg attctatcta actatccaac
     6901 aaactttgct aatggcaatc cgattatgta tgctaatagc aagggaacag gaatgatgac
     6961 cttgggagaa gctctgaact attcatggaa tatccctgct tactggactt atcgtatgct
     7021 ccgtgaaaag ggtgttgatg tcaagggtta tatggaaaag atgggttacg agattcctga
     7081 gtacggtatt gagagcttgc caatgggtgg tggtattgaa gtcacagttg cccagcatac
     7141 caatggctat cagaccttag ctaataatgg agtttatcat cagaagcatg tgatttcaaa
     7201 gattgaagca gcagatggta gagtggtgta tgagtatcag gataaaccgg ttcaagtcta
     7261 ttcaaaagct actgcgacga ttatgcaggg attgctacga gaagttctat cctctcgtgt
     7321 gacaacaacc ttcaagtcta acctgacttc tttaaatcct actctggcta atgcagattg
     7381 gattgggaag actggtacaa ccaaccaaga cgaaaatatg tggctcatgc tttcgacacc
     7441 tagattaacc ctaggtggct ggattgggca tgatgataat cattcattgt cacgtagagc
     7501 aggttattct aataactcta attacatggc tcatctggta aatgcgattc agcaagcttc
     7561 cccaagcatt tgggggaacg agcgctttgc tttagatcct agtgtagtga aatcggaagt
     7621 cttgaaatca acaggtcaaa aaccagggaa ggtttctgtt gaaggaaaag aagtagaggt
     7681 cacaggttcg actgttacca gctattgggc taataagtca ggagcgccag cgacaagtta
     7741 tcgctttgct attggcggaa gtgatgcgga ttatcagaat gcttggtcta gtattgtggg
     7801 gagtctacca actccatcca gctccagcag ttcaagtagt agttctagcg atagcagtaa
     7861 ctcaagtact acacgacctt cttcttcaag ggcgagacga taattctcta aatgaagtgg
     7921 ccaatcaatg gattgccact tttttccttt cgtgttacaa taagggtatg aatcagtatc
     7981 agaaaaagat tgttaacgga aaaatttatt cgctcctatc cggcttaata tggggaatct
     8041 gtggaatttt aggagagtac ttctttactc attatcaggt gtcttcgggc tggattacct
     8101 ctatgcgttt gacactggca gggagtcttg tactcatttg gtctgcaata caattaaaat
     8161 cgcaagtgct agatatttgg cgagacaaga aaaattacct gcccttttta gcctatgcta
     8221 ttttggggat tttttcagtt cagtattttt tctatctctg tgtagaatac tcaaatgcta
     8281 cgacagcaac tattttacag tttattagcc ctgtctttat cctcttttac aatcgcttgg
     8341 tttatcaaaa acgagcgtca aaaagcgctg ttttctatgt tttggttgcc atgctgggtg
     8401 tttgcttgat ggcgacaaag ggagatctct ctcagttatc catgacgccg ctagctctta
     8461 taacaggttt gctgagtgcc atgggtgtta tgtttaatgt tatcttgccc caaccttttg
     8521 ctaagcgtta tggttttgtt cctacggttg ggtgggggat gattttggca ggtttgttta
     8581 gcaatgtcct ctcgccggtt tatcagcttt cctttactct tgatatttgg agtatcttga
     8641 tttgcctcat tatcgctttc tttggaacgg cttttgcttt tttcatttcc atgaaggctg
     8701 tgtccttggt ttctcctttg gtggttgccg ttatcagtgc cagtgaacct ctctcttctg
     8761 ctctcttgag tgttttgttc ttaggattag tagtggattg gtccctcctt ctagctatag
     8821 ccttgattat tttacccatg atatttttgt ctatagaaga agcgaaagaa agtagataaa
     8881 aagtcttttc ttaattctaa aagtgtgcta tactagaata gtcaataaaa cacggggaga
     8941 tagttgtgaa gagtgttttt aggttgtatc ggtaggggct tgcttttacc gacagcacgt
     9001 tttatctcac atccctaaaa aatcatacct aaaaacagac aaaaaggttt caaatttgag
     9061 gccttttttg gtagaatagg tatcattata acgaaccagg aggcacctat gactgctaca
     9121 aaaatgaacg ctcaagaaat tatccaattt atcgccaatg ctgaaaagaa aaccagtgtc
     9181 aaagtaacct ttgaggggca actcgcaact gctgtgccta gctctgttgt caaactagga
     9241 aatgtcctat tcggagactg gaaggacgtg gctccgcttc ttgaaggttt ggtagaaaat
     9301 caagattatg ttgtcgagca agatgctcgt aattctgcag ttcctttgct agataagcgt
     9361 gctatcaacg ctcgtatcga gccaggtgct attatccgtg accaggtgga aattggtgac
     9421 aatgctgtta tcatgatggg agctgttatc aatatcggtg ctgaaatcgg tgctggaacc
     9481 atgattgaca tgggtgccat ccttggtggc cgtgccatcg ttggaaaaaa tagccacgtt
     9541 ggtgcaggtg cagttttggc aggtgtgatt gagccagcta gtgctgaacc agtccgtgtc
     9601 ggagacaatg ttcttatcgg tgctaatgca gtggttatcg aaggagtcca aatcggtagt
     9661 ggttcagttg tcgcagcagg agctattgtt acccaagatg tcccagaaaa cgtggtagta
     9721 gcaggtgttc cagctcgtat tatcaaagaa attgatgccc aaactcaaca aaaaacagcg
     9781 ctagaggatg cgcttcgtac cttgtaattg taaaagtaaa aaagaggcgg aacccttttt
     9841 ccagcctctt tctgctatat cggaggacag atagatgtta gatttgattc agactagacg
     9901 agctttacac cagattccag agattggctt ggaggagttc aagactcagg cttatttgct
     9961 ggatgtgatt gagaaattga ctacgggcaa ggattttgtt caaattcgta cttggcggac
    10021 agggattttg gtctacctgc agggaagtca gccggagcga accattggtt ggcggacaga
    10081 tattgatggc ctgcctatcg tcgaacaaac aggtctacct tttgcttctc aacaccaagg
    10141 tcgcatgcat gcctgtggcc atgattttca tatgaccatt gccttaggct gtcttgagcg
    10201 cgcccttgag gagcaaccca agaataatct gctctttcta tttcagcctg ctgaagaaaa
    10261 tgaagctggt ggtatgctca tgtatgagga tggtgctttt ggagattggt tgccaaacca
    10321 attctatggt ctccatgttc gtccagattt gaaagtcggc cagattgcga ctaatactca
    10381 tacactcttt gcaggaactt gcgaggtgaa gatccgtttc aaaggcaaag gtggccacgc
    10441 agcttttcct catgaagcca atgacgcctt ggtggcggct agttactttg tgacccaagt
    10501 gcagtcagtt gttagccgca atgtcaaccc aatcgaggga gcagtggtga ccttcggcgt
    10561 ttttcaagct ggaaccacca acaatgtcat cacagacaca gcttttttac atggaactat
    10621 tcgtgccttg acacaggaca tgagtctctt ggtgcaaaag agggtaaaaa cagttgcaga
    10681 aggggttgca gctgcctttg atatggaagt cgaagtggaa ctcaaacaag gtggatacct
    10741 acctgttgag aacaatccag ccttggcgcg tgaactgatg gacttctttg acgaaaaaga
    10801 cggaatcgag ttgattgata tcgaacctgc tatgacaggc gaggactttg gttatctcct
    10861 ttcaaaggtt gatggcgtga tgttttggct aggtatcgat agtccctacg ctcttcatca
    10921 ccctcaaatg agtcctaagg aagaagtctt agctattggg gtggctgcgg tgtctagttt
    10981 cctgaaaaag aaggcagcag aatagagggc ttgtctatga aatcggaatt acgcaagcaa
    11041 gtcttgcatg aaatgaaggc tatatctcaa gaacaaaaac agtttataga tcaaacttta
    11101 accgagcggc ttttacacca tcccttctat caagaagcca aggtcatcgc aacctatctc
    11161 tcttttcctc atgagtttca aacgcaggaa ctgattgagc aggcgctgaa ggacggcaag
    11221 aaggttttaa tacccaaaac ttatcccaag gggcgcatgg actttgtggt ctatgatccg
    11281 caacagttgg taaaaacttc ctttggatta ctggagccac agggagattt ggaagtggtg
    11341 gatgcatctc agattgattt gattcatgtt cctggcctgg cttttacgac gaaaggatat
    11401 cggataggat acggtggagg ctattatgac cgctatctgg aacatttttc tggtcgtact
    11461 ttgagtacga tatatccttg tcaaattcag gactttatac ctgaatttga caaggatatt
    11521 cctgttcagg aggtattaat tgatgaagga aatctttgat agacgttacc ctgtgacgag
    11581 tttcttcctc ttagtgacgg ccttggtatt tttactaatg ttggtcactg caggcggaaa
    11641 ctttgacagg gcagatacat tatttcgatt tggagccatg tatgggccag ctattcgcct
    11701 ctttcccgag caggtttggc gtctcttgtc tgccattttt gttcatattg ggtgggaaca
    11761 tttcattgtt aatatgcttt cactttatta tcttggaagg caggtagagg agattttcgg
    11821 ttctaagcag tttttctttc tctatctctt atcaggaatg atgggcaatc tctttgtttt
    11881 tgtatttagt cctaaatcct tagcagcagg cgcctctacc tctctttatg ggctatttgc
    11941 cgcgattatt gttcttcgct atgcaactcg caatccttat atccaacagc tagggcaatc
    12001 ctatctgaca ctttttgtgg ttaacattat tggaagtgtt ctgattccag gaatcagcct
    12061 agcaggccat atcggtggtg cagttggtgg cgcatttcta gcagttatct ttccagttag
    12121 aggagaaaaa cggatgtata acaccagcca gagattagga gcggtagtct tgttcgtagg
    12181 actcgccatt ttgcttttct acaagggaat gggaatgtga cgactaatac tcaatgaaaa
    12241 tcaaagagca aactaggaag ctagccgcag gttgctcaaa gcactgcttt gaggttgtag
    12301 atagaactga cgaagtcagc tcaaaacatg gttttgaggt tgtagatgaa actgacgaag
    12361 tcagtaacca tatatacggt aaggcgacgc tgacgtggtt tgaatttgat tttcgaagag
    12421 tataatatgt aaacttgaat aagaaaaaag ctacttaaaa aatgagtagc ttttaatgtg
    12481 ttttggaacc attcgaaaca acacagctct aaaatagtta ttagcgttgt ggaggttctg
    12541 caatgcctcc cccagtgttt ccaagataat tcccattttc gtcaaacaca tctccattgc
    12601 tctcattatg ataagtatct cttacatgtc cactagcatc aatattctta tattttttat
    12661 agtagccatt atttgctccg ctgtttgaat ttctagatga atattgttct gatgatccac
    12721 catttgttga actatctcct gaaccagaat aatcttgacc tccataatta ttcgaatatc
    12781 cattagtttg ttcagatgaa ctataatcta catttgaatt ggaagatcca ttataagact
    12841 cattagaagc accactagaa gaattacttt gactagaaga ttcctttgct ttttcagcag
    12901 ctttttcttc ttctttcttc aaaagttcga tttgtttttg atatttatca acaagatcat
    12961 ttagagattt aagagtttct tccaaattct cttgtttaaa agtttcatca acttttttga
    13021 taaaatcaat ttgttttgta atttctttca catttgtttc aagagttgct ttatctttaa
    13081 acttctcatc agaatcttta acatcataag ccaaaccttt ttgcaaatga aaatctacaa
    13141 ttgctttaga tgattctttg actttgttat taagatagtc tttacctgca tcggttttaa
    13201 gtgaagtttc agatagttct tttaatttag aaatagattc atctgaagat gcatcaaatg
    13261 attcaaatag tttatcaact tgttctttgt ctgatttgct atctttttct aattgtacaa
    13321 tttttgcttg ttgttctgct tgttgttggt tatatgaata ccatccaaac cctaatacca
    13381 atacaacacc aactgcaact gatgaaatga ttactttttt attgttttcg ataaattcta
    13441 caaatttgtt ctccataatc ttttactttg atagtgtccg caattacttt aaaattaaaa
    13501 ttacacctat ctttctccct tattatattt ttattaatag cttccacaaa attaaacaac
    13561 ttatgttcgg ctcggttgat ttaatttaac catgactact ccaacagtta agtcaaattt
    13621 aaaattattt attattttgg aaccattaga aacaacacag caaagttaaa ataaggcttt
    13681 gtccgtacac aacttgaaaa agtgcgcacc gattcggtgc ttttttgttt tactatacta
    13741 atttattcta ctcttatttt aatgaaaaaa caaatacaag tatagcgttt tcattagttt
    13801 ttattaaata ggtagattca agtagagaat ttacttaatt tatgtgtggc taatctttat
    13861 ataaatgatt ttgttattcc ttctcagtca attcttttcc aagttggatg aggtaattct
    13921 tcaaatcatc tttgacttgt gggtgtttga gggcgtagtc gatggatgtt ttcatgaagc
    13981 caaacttgtc tccaacgtcg taacgagctc ctgtaaattc acgggcaaaa acacgctgtg
    14041 ttttattaag agtatcgatt gcatcagtaa gttggatttc atttccagca ccaggagctt
    14101 gcttttcgag aatctcaaaa atttcaggcg tgaggaggta gcgtccgata atagcaaggt
    14161 cgctaggagc gtcctctgga gctggttttt caacaaaggt ttcaacactg taaagaccat
    14221 cttttccttc gccttgcgga gcaataaccc cgtaagcaga tacttcgtca tgagggactg
    14281 gcatgacagc gatagtagac gcgtgggtac gctcgtagtc atccatgagt tgtttggtaa
    14341 gtggaacagc cttttcgtct gtgatatcca tcaagtcatc accaagcata acgacaaaag
    14401 gttcatttcc gacgaaagcc ttggcttgca aaacagcatc tccgagaccg cgtggatgag
    14461 tttggcggat aaaatgcaga cgcatgccag ttgtttcatc aactagcttc aaaagatctg
    14521 ttttcccttt ttctttgagg ttatattcca attcgaagtt tgaatcgaag tggtcctcaa
    14581 tagaacgttt tgatttacca gtgacaacta gaatatcttc aatacctgat ttgagagctt
    14641 cttccacgat aaactggata gttggtttgt ctacgattgg caacatttct ttggcaaggg
    14701 ccttggttgc tggtaaaaat cgagttccta gtccagcagc agggataact gcctttctaa
    14761 cttttgatgt cataaaaatc ctttctatag agggttaaga ccactcattt tctgctttaa
    14821 attcattgtt catgatgtca taaatggcat ctttgatatt ggttccgtga taaataactt
    14881 ggtaaatagc ctgtgtaatg ggcatataga ctccaagttc ttgggctagt tcataggctg
    14941 ctcgagtcgt tgaaattcct tcgattacca tgcccatatt agcttctata tcagctaggg
    15001 attctcctcg tccgagagca tctccagctc tccagttacg agagtggatg gaagttcccg
    15061 ttacgatcaa atctcccaca ccagataagc cgctataggt caatggactg gccccgagtg
    15121 ctacccctag gcgggtgatt tctgctaaac ctcgagcgat gatggctgcc ttagcattat
    15181 caccaaatcc aagaccatgt aaagctccag caccgacagc aataatattt ttaagagcac
    15241 cagcagtttc aaccccgata acatccgtat tggtataaag tcggaagtag tgattactaa
    15301 atagcttctg aacgtattga gctgtttgta aatctttaga agcagcagtt attaaagtta
    15361 ggtcacgcac aatggtctct tctgcatgac taggccctga aacaacgacg atatcactac
    15421 ggagatgttc aggaatttct tcttcaagaa tggttgataa tcgtttatgg ctatcaggtt
    15481 ctaatccctt tgatgcgtgc atgatgataa ccttatggtc caaggtttgt gcaacttgct
    15541 gggcaacaag tcgtgtcact tttgttggga caacaaacaa aatcgcaccc acatttttca
    15601 atgtttctgc taagtcggtg taggcaatga tattttcgtc tagaacgaca tctttaaagt
    15661 agtgcttatt agtatggtgt gtattaattt cattgatttg ctcgggaaga tttccccaaa
    15721 tacgtacctc gtgtccattg tcatttaaga cttgtgaaag ggcggttccc caagaaccag
    15781 gccccaagac ggcgacggtt tgtttttcca tgatttcctc cttgatagag ttctttctct
    15841 tattctatca tattctaggg gattttgcac ttgcattact ggggtatggt ggaaaaatac
    15901 acaaaaaccg agacgaaggg ataaactctt agtctttagt taaagtgaga agaaatctga
    15961 tagatagttt gattcagtat atgcttataa tagaggtaaa caactcagga ggttcttgta
    16021 ggttgcgaga gtttggcgaa aaaattaaaa gattacgttt ggctaaaaaa atcagtcgtt
    16081 cagaattttg tggtgatgag tctgaattaa gtatccgtca attaattaga attgaaaatg
    16141 gagaatccag accaacacta acaaagttaa aatatatcgc tgaacgtttg ggggttgaag
    16201 attacaagtt gatgccaagt tatatagagt tggataagga atacctagaa ttgaagtatt
    16261 tcttgatgag aactcctaca tacgaagatg aaactatcgc ccaaaagaaa gagagtgttt
    16321 tgataagatt tttgaagagt attatgatag gctacctgag gaagaaagat ttatcatccc
    16381 aaattattca tatctggcac taacgaacta cacagttcaa aaattaccag aaaagctagt
    16441 tgaaatactg tccttctggt gattttgctt aatttctgat gttcgagaca taattgagga
    16501 gatttgtctt gattttttcc taaaaaaggg tagactcctc ccttggtttg atgctcgatt
    16561 tttaaaataa ggtgacttat tctgttaggc atgttttccc ctgccctcta gctctatatg
    16621 gaacagggag tatcagattt atctggcaga ttgtatcaaa taaggcttga aactgttttg
    16681 aataggcgta tagacttgag aatttgagaa tatgtcgtct agcagtagag acatattttc
    16741 cggcaatatg aaggaagaga cgtcgaaaac gcttgatagt caaggccttt acttcatcac
    16801 cagctagctg ctttaaaaag aggtagagat tataagccag acagcccatc atcatacgta
    16861 cttcattctt aatcatggtc gaactatctg tcttatcccc aaagaatcct gctttccttt
    16921 ctttgataaa gttttcagca ttccctcgtt cacgatagaa ttgaaacagc tcctcactat
    16981 ctacactgct taaattggtc aaatgaactt ttttacaacc ataaaaaaga gcacttaatc
    17041 gtactcttct cttttaagga ttaaaggatt agttgaatta attcaaatcc actagttctc
    17101 ctgtttctag gtagactagg cgttcacaaa tgttagcaat gtaatcagcg aagcgttcca
    17161 gatgccctat gatataaagg tattgtgttc cattgggaat tgaggtttct tggtctttca
    17221 taagtccaat gatttccttt gataaggcat aataatattg gtcaatctgt tcatcttttt
    17281 gagcaatact aatagctttt gaggcttggt gcaaaggaaa ggcaaccaat aaatcggcta
    17341 gcatgctgag ggataattta cccatttggt gtaactgttc ttcgtcaggg gctagttgat
    17401 tttcttttag ttgcaaaaca gctttggcaa tgcctgccat atggtctccc atacgttcaa
    17461 ggtctgaaca agaagacatg atgctaatca caaatcgaag gtcagacact tgtggctgct
    17521 gcaaggccaa caaacgggca caggtcaatt cgatagcgct ttgaccttgg ttgatagcat
    17581 gatccttatt gataattagc tctgccatct ccttgtcttt ggaggctaag gccagtaagg
    17641 cttttgaagc tgtttcaagg acaagttgcc ctagtcctaa aaaggattgt tctaattcat
    17701 gcaattctaa gtcaaattga tttctcatag gtttttcctt ccttatccga accgtcctga
    17761 aatatagtct tctgtgcgct gatcttttgg attggtaaac acgtcaacgg tatctccaaa
    17821 ttcgcaaatt tctcctgtta agaaaaaagc agttttatct gaaatacgtg aagcttgttg
    17881 catgttatgg gtaacaatga taatcgtata atcctttttt agttgctgaa tgaggtcttc
    17941 aatttttaaa gtggagatag ggtctaaggc tgaagtcggc tcatccatta acagaatatc
    18001 aggttctact gctaaagctc gcgcaatgca aaggcgttgc tgctgaccgc cagataagga
    18061 catggcactc tttttaagat catctttgac ttcttcccaa atggctgccc cttttaaaga
    18121 tttctccact aaggcatcta attgtttttt gtctcgaata ccatgtgtcc ttgggccata
    18181 agccacgtta tcatagatag acatggcaaa gggattaggc tgttgaaaaa ccatccctac
    18241 acgcttacgt agctgattaa ggttgaattt gctactataa atatcttgct catctaagag
    18301 gacttggcct tcaatatggc aagaaggaac caaatcgttc atccggttaa gggtttttag
    18361 aaaagttgat ttgccacaac cagatgggcc tatcaaggca gtaatctgtc tttctggtaa
    18421 ttgaatcgaa atatttttta aggcttgaaa atccccgtaa aataagtcta ggtgtctgac
    18481 tgaaaatgtt cccatactag gaagctcctt tcacaagttt tcgagataat aagcttgata
    18541 gagtatttat cattaaaaca gtaataatca aaatcacgcc ggtagcatag gcttcattga
    18601 catgtagccc ctcacttgac agcatataca tatgtagggc tagagaacgg cctgaagaca
    18661 tgagactact tggcgtattg gtagaggtac ctaatgtata catgagggca gctgtttcac
    18721 caacgatacg gccaatagct agtatcactc cagctaaaat acctggcatg gcaactggta
    18781 gaacaattct aaaaacagtc cgtaacttac ctgccccaag tccataactt gcttgacgca
    18841 tgctatcact aacagataaa agggcttctt ctgttgagcg aataatgact ggcaacacca
    18901 tgataactga ggttaagatt cctgataaca gagagtattg aaaacctaag aagactacaa
    18961 agaagagcat gccaaacaga ccaaaaacaa tggaaggaat cccagataag gtatctgagg
    19021 ccaatcgcat gattttaaca caaagggaat ctttttttgt atattccaca agataaaaac
    19081 cagcaaaaat ccctatgggc aaggctaaaa gaagagcacc aaagaccaga ataacggtgg
    19141 aaataatcgc tggcataagg gaaatgttct cagaagtata agtccaagaa aagagggata
    19201 gacttagatg aggtaagcct ttgatgagga taaaaccaat gattaaaaag agagagccaa
    19261 aggttaaagc tgaaaaacaa taaacgagaa gttttagcag gtatttactc ataagatgat
    19321 tttcctttca agtaggcaaa gtaggcatta atcaagagaa taaggaaaaa gagaactgct
    19381 gaggttgcaa taagggcttc cctatgctga cctgatgcgt aagccatttc cagaacaata
    19441 ttggttgtta aggttctggt tcctgaaaag agtccacttg gaataatcgg ctggttgcct
    19501 gccaccaaaa taactgccat ggtttcacct actgcgcgac caattcctaa aataactgct
    19561 gataaaatac cagatctcgc agctggcaag atgacactaa aaatactccg ttcatgacta
    19621 gctcctagag ccaagctacc agaataatac gttttgggaa ctgttcggat agcagattct
    19681 gacaaactga taatggttgg caaaatcatt attcctaata gtaacgaagc ggttaggaca
    19741 ctcatgccat ttcctaaaaa ggttctaatc caaggcacca ataattgtag gccgaaaaaa
    19801 ccataaacaa tagatggaat ggccgccatc aagttgatag ctgattttaa gaagccatag
    19861 acgggctttg gacaataata aaccataaac accgatgtca agatgcctgt tggcacccca
    19921 atcacaatcg ctcctaaggt aattaataag gaaccaacga tcattggtaa aataccatag
    19981 cttgccggaa tgttcgttgg cgaccaatca ctgcctaata aaaaacgggc aaagccgtag
    20041 ttagccatga aaggtaagcc attactaaaa ataaagaaac agattagcaa aatagctaca
    20101 acagctactg ttgcactcat gaaaaaaatt gccctaaaaa ctgcttcttt gaaggcttgt
    20161 tttgtcacat cttgtccttt ctagtgaaga aagtaaggga gatacgacac ctccctactt
    20221 gccttcttta tcttattgta cgatgaaacg tctgcatctc tttagagatt tatggagcaa
    20281 acattttatt taatcttgtc ccaggtggtt aatttgccac taaaaacgtc tgcaagttca
    20341 gccatactga cttggcttgc cttattgtca ttattgacca caacagcaat accgtctaaa
    20401 gcaatagcat catgggtgag actcttacct tcttcaggag ttagttccct agaaaccata
    20461 ccaatatcag cggttttctc cttaacagcg gtaatacctg ctgaagaccc attagaggta
    20521 atatcaatcg taacttctgg attttctttt ttataagctt ctgctaattt ttccattaaa
    20581 gaagatactg aagtggaacc tacaacagac aacttgcctg ataagtgttg gcttgtatat
    20641 tctgtggttt cggttttaga ttcaataaat ttattatctg tgaccacttg ttgaccttgt
    20701 ttggagtgga taaagctgat aaaatcttga cctagcttgg aaagattaga agaccaaaca
    20761 atgttgaagg gacgttgaag agggtattca ccatctaaaa ctgtgtctcg actagccttg
    20821 acaccatcaa tctctaaagc cttgacagat ttcgttaaag atcccaagga gatgtagccg
    20881 atagcattag cattcccttg aactgctgag agaacacctt ctgtactatt ttgaatcaca
    20941 gctgttttgg cagtgttgtc aattttttta tcaccgtctt ttttgagaat ccctgtgatt
    21001 tctgtgaagg caccccgtgt tccagagcca ttttctcgtg aaatcacctc aatcgttcct
    21061 gaagctgact gtttggaagc agctgactga ttgccacagg caacaagccc aaatcctgat
    21121 aagccaatgg ctgcaagagt aagcattttt ttgaatttca taataatcac ctttatctct
    21181 atgtattttt cttgtgtagg cttactacat ttatagtcta acaagtcttt gtaaaggttt
    21241 atccctgatt catgtaaaga ttgtgtaaag aatcaaaaaa agccactttt gaaaaatggc
    21301 tgctcctaaa aatagcttta aaaattatta gtcctgtgcg aaagattggt taggaagaaa
    21361 aatcgtgaag caactgcctc tgccaagctg actcgtcacc gtgacttggc cacctaataa
    21421 ttgactgagt tctttgacaa tggcaaggcc aagaccagtg ccaccagttt gtctgcttcg
    21481 acctttatta actcggtaaa aacgttcaaa aatacgatcc tgctctaatt gactaatacc
    21541 aatccctgta tctgatacag aaatcttaat gccttcgttc accttttggg tcttgacctc
    21601 aattttcccc ccttgttcag tgtaacggat ggcattggat aaaagattga gtaagatttg
    21661 ggaaagtaat tgactatctg atacgagggt gacatcatct ggcacctgca cctttagctg
    21721 taaatccttc ttcttgagct gaggttgcaa gctttgagtc aaatcctgta caaattctgc
    21781 caaagaaagg gtcgtccatt gtataggcat ttgttgagcc ttagataagg taagaagatg
    21841 ctcaacaata tgctcaagac gcaaactttc tttgtaaata atgtctagaa agtcatcctt
    21901 gagcgcttct tcttcagctg acatcccctt aatggtttca gcaaagccct taatcgaagt
    21961 aactggtgtc ctcaattcat gggaggcatt tgagacaaag gctaaattta acttttcata
    22021 agttctaatc gttgttaaat catatagcaa gacgagcaca gcttccacag attgggtggg
    22081 gctaaaaacg ggaactgctg tcacttctaa aatcaagtca ccctcatgaa acccacttac
    22141 ttcttgtttt aaccttgttt tttgatcaaa ggcttggtga actaaattcc gaatatccat
    22201 ccgtttgagg tcatcaagtg aacttatgtc gccgtccaca tcgggaaaat aatgaggcag
    22261 agagcgactg gataataaca tctgaccttg agcggaaact aaaaacgtcc ccatggttag
    22321 gtgcgacaga agaacctcca ttgtttcggc taggtccttg tattgctgat cctgttggga
    22381 gactttggtt tttagggcag acacatactg agccaaagac tttaagtctt cttgcccttt
    22441 ttctaaaaag tattcactac tggtcaagag aggttggtgc aaggtctcaa aagcaacttc
    22501 ccatttccaa aggcaaaaga gccagtagcc accttgtccc aaagaaaggg ctagaagaaa
    22561 gagaccgatg cctttactga tccaagttaa tgccatccct gcaatcagaa tgaggctaac
    22621 acttagattg actagccaaa attgaaggta gcgtttcatc tataactcct tgaacttata
    22681 accataaccc cgaatggttc gaataaattg aggggcttta ggattgtctt caattttttc
    22741 cctcaactta ccaatatgaa cgtccaccaa acgtgtttcc tgcccaaagt cataccccca
    22801 gatacgttcc aaaagacgct ctctagtcag tgtcatgttg ggatgtttca taagatagag
    22861 caagagttca aattcttttg gggtcaaact cagtaactta ttcgccttgt agacttcatg
    22921 acgctcaggg tatactttca aggtcccaaa tagccaagaa tcgtcagcga tattatctga
    22981 atcatctcct tcttgttctc ctttagttcg cctgaggaca gccttgacac gcgccagcaa
    23041 ttctctaggg ctaaaaggct tggtcaggta gtcatcagcc cctaattcca aggccaaaac
    23101 cttatcaaat tcatcacttt tcgcagaaac catcataatt ggagttttga cgcctttggc
    23161 tctcagccgc ttacaaactt ccatgccatc taattgtggt aacatgatat caagcaagat
    23221 aaaatcaaag ggttctgttt ctgccaaagc taaggccttc cgtccatttg ttaccaattg
    23281 agtagaaaag ccttccttac ttaaatggta gtcaagcaat ttcagaatgt gttcttcatc
    23341 atccactaat aagacttgtt ttgtcatcta ttatctccta ttggtaacat tataacacaa
    23401 ttatcagaaa tcctaacatt gctaaatcag attaaatttg cttatcaaga ctagtatctg
    23461 gtcaaacgct caatcatctc cttgtgctct ggataggtcg ccagtagatc taccctttca
    23521 aataattcaa aatcctcaaa ttcaaaacca ggagcaacaa gacaagaaac cagagcatca
    23581 tccttatcaa ctgttgatcc ccaaatagtg cccttaggaa cacagtagtg aagttgttgc
    23641 cctttggata tgttcaggcc taaagtgact gtttcgtagt gaccatctgc tgtaatcatg
    23701 tgaacagtaa gtggggatcc tgcatgaaaa taccagattt catctgctgt caatcggtga
    23761 aaatgtgaag gattcgtttc ttcttaataa gaaataaata ctggtataaa gcgcccttcc
    23821 cttaccagca aggtttatag tgtctgaagc tttttttgtt tgtctaacat agccaccttc
    23881 aacatgggga gctaactcta gagttcttat caagtcttct ttatccgtcg gagccaatgg
    23941 gttgaagtaa ctcttgttca aagtggtgtt acgatttcaa gaactcctct cagctctgag
    24001 gacacggtaa tgattgatgc gacggaagta caaatcaatc gccctaaaaa aagaattagc
    24061 gaatgattct ggtaaaaaga aatgccacgc tatgaaggct caagcgattg tcacaagtca
    24121 agggagaatt gtttctttgg atatcactgt gaactattgt catgatatga agttgttcaa
    24181 aatgagtcgc agaaatatcg gacaagctgg taaaatcttg gctgacagtg gttatcaagg
    24241 gcccatgaag atatatcctc aagcacaaac tccacgtaaa tccagcaaac tcaagccgct
    24301 aacagttgaa gataaagcct gtaaccatgc gctatccaag gagagaagca aggttgagaa
    24361 catctttgcc aaagtaaaaa cgtttaaaat gatttcaaca acctatcgaa atcatcgtaa
    24421 acacttcgga ttacgaatga atttgattgc tggcattatc aatcatgaac taggattcta
    24481 gttttgcagg aagtctatta tttggttagg tgaattagtg aagcgtttag gcaagtgtct
    24541 ctggttacga cgtcatgacc tctaaatcga ttatatttag gggtcatgac tagtgaagca
    24601 gttagctagt tcgcatataa gcggctagcg tctaacaatt aggaacttta gttccaataa
    24661 ctttaagatt acgacgtttt aggacataaa tcgatcatat ttatgtccta aaactagtga
    24721 agcgcctagc caaagtccga ataggatttg gcgttagtta cttagattgc tttgcaatca
    24781 agtaactttg gcgatttaca tcttctctgg cgcttctact ccaagcaagc gaagggcttc
    24841 tttgagaacg actgcggttg cgtagctgag ggctagacgg ctgtcgcgtt ctgggctttc
    24901 atccaagata cgtgtatgtg catagtattt gttaaaggat tgagccaggc taattgcaaa
    24961 tttagcaatg atagaaggtt caaagttatc tgccgcacgg ttgataatac gtgggaagtc
    25021 ttgaatgagt ttaatgattt cccagctttc agtatcattc aagctatagt tgccagctgt
    25081 ttctggtttg aaatcggctt tgcgtaagat agattggata cgagcgtagg catattgaac
    25141 gtaaggtcca gtttcaccct cgaaggatac catagcctct aggtcgaagt cgtatccatt
    25201 tgtacggtcg gttttgaggt catagaattt aatggctcca accccaacag catgtgctac
    25261 ctgatcttta ttttccaatt caggattttt agcctcgatt tggaccttgg cacggctaac
    25321 agcctctgca acagtaggct ctagcaagat gacattccct ttacgagtag agagcttctt
    25381 cccttctttt gtaaccaagc caaaaggaac gtgagtaatg tcgtcactcc agtcgtagcc
    25441 catctcttgc aagacagctt tgagctgttt aaagtgggca gattgttctt gaccaacgac
    25501 atagatagat ttagcaaatt ggtattcgtt tttacggtaa agggctgcag ccaagtcacg
    25561 tgtgatatag agagttgcac catcagactt cttgatgagg gctggatgtt caattccgta
    25621 tttctcaaga ttcacaactt gggcaccttc tgattcaaga agtagtcctt tttcagaaag
    25681 aatgtctaca actgcatcca tcttatcatt gtagaaggct tctccgttat agctgtcaaa
    25741 ttcaaccttc aattcattgt aaaggcggtt aaattccact aaactttcat cgcggaacca
    25801 ttgccaaaga gcgagagctt cctcatcttc attttcaagt ttacggaacc attcgcgcgc
    25861 ttcttcatcc aagctagggt cattttcagc ttcagcgttg atgcggacat agagtttaag
    25921 gagttcatcg attggatgag cttttacagc ttcttcgtcg ccccattttt tgtaggcaac
    25981 aatcaacatc ccaaattgtt taccccagtc tcccaaatgg ttgaccttga ccgtttgata
    26041 accgattttt tggaaaatat gtgacaagct atctccgata acagttgaac gcaggtggcc
    26101 aatagaaaat ggtttagcga tattcggact agacatgtcg ataacaacat tttcttgttt
    26161 gccaatattt tggtcagcat agtgttcttt ttcagtggta acagcttgca atacttgagc
    26221 agaaatggca gatttatcaa ggaaaaagtt aacgtaaggt cctgttgcga caactttttc
    26281 aaaggcttgg ctgttcattt tttcagccag ttcagccgca atcatttgtg gtgctttacg
    26341 ttcgactttg gcaagagaaa aagcagggaa agcgatgtca cccatttctg agtttttagg
    26401 ggtttccagt aactttaaaa tagcctcttg gtccaggcta tcaatgatgc tagccaattc
    26461 gctagcaatc aattcttttg tattcattaa gagctccttt ttggactttt ctactatttt
    26521 aacacaattt taaagaaaga agaaaaaatt
//
LOCUS       DAVKWZ010000026        25478 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000026 DAVKWZ010000000
VERSION     DAVKWZ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25478
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.026"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(161..379)
                     /locus_tag="VW854_001129"
     CDS             complement(161..379)
                     /locus_tag="VW854_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0262371.1"
                     /translation="MALNYKPLWIQLAKKGLKKTDVIAMAGLTTNVMAQMGKDKPITF
                     KNLERICKALSCTPNDIISFEDNFSDEE"
     gene            complement(625..903)
                     /locus_tag="VW854_001130"
     CDS             complement(625..903)
                     /locus_tag="VW854_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262372.1"
                     /translation="MKKDKEVYDKVCEYLSPHLNKDMEVLELACWFRVITVIEANSYV
                     NIRSSRLLPKFKTQKINSWCAAYNIHFNNGYCKQHTNEFKDSLKSLIL"
     gene            complement(1117..2748)
                     /gene="rlmD"
                     /locus_tag="VW854_001131"
     CDS             complement(1117..2748)
                     /gene="rlmD"
                     /locus_tag="VW854_001131"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV0262373.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGLAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(2822..3796)
                     /locus_tag="VW854_001132"
     CDS             complement(2822..3796)
                     /locus_tag="VW854_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEV0262374.1"
                     /translation="MRKKLFLTSAAILWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGVEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDIRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDSDKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(3870..4484)
                     /gene="pmp23"
                     /locus_tag="VW854_001133"
     CDS             complement(3870..4484)
                     /gene="pmp23"
                     /locus_tag="VW854_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEV0262375.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNRTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            complement(4484..5458)
                     /locus_tag="VW854_001134"
     CDS             complement(4484..5458)
                     /locus_tag="VW854_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEV0262376.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            complement(5466..6722)
                     /locus_tag="VW854_001135"
     CDS             complement(5466..6722)
                     /locus_tag="VW854_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEV0262377.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPKTELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYEE"
     gene            complement(6783..7214)
                     /locus_tag="VW854_001136"
     CDS             complement(6783..7214)
                     /locus_tag="VW854_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0262378.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEQEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKVQKRFIRIF"
     gene            complement(7216..7818)
                     /locus_tag="VW854_001137"
     CDS             complement(7216..7818)
                     /locus_tag="VW854_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEV0262379.1"
                     /translation="MMDRIRQELENGGAVVLPTETVYGLFSKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPAYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(7802..8641)
                     /gene="prmC"
                     /locus_tag="VW854_001138"
     CDS             complement(7802..8641)
                     /gene="prmC"
                     /locus_tag="VW854_001138"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEV0262380.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALDLASENAKNQNLQ
                     IFFKKSDCFTEISEKYDIIVSNPPYISREDESEVGLNVLYSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKRVRTLKDQFGQNRMVVVDD
                     GQD"
     gene            complement(8641..9720)
                     /gene="prfA"
                     /locus_tag="VW854_001139"
     CDS             complement(8641..9720)
                     /gene="prfA"
                     /locus_tag="VW854_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEV0262381.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEELAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(9730..10209)
                     /locus_tag="VW854_001140"
     CDS             complement(9730..10209)
                     /locus_tag="VW854_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0262382.1"
                     /translation="MTIELRDVTMENYFDVLNLDVKEYQKQFIATNAISLAEAYVYTK
                     NGDFVAPLAVYDNDAIIGFVMIAYDKKIGISSGNYLLFRFMIDKNFQNQGYFKPIMDK
                     VLDYVRTAPAGLSNKLWLSYEPENEQARFCYLSYGFKETGEISENEVVAIYDLTIEK"
     gene            complement(10199..10798)
                     /locus_tag="VW854_001141"
     CDS             complement(10199..10798)
                     /locus_tag="VW854_001141"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEV0262383.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPMITSNKEQNYDN"
     gene            10921..11103
                     /locus_tag="VW854_001142"
     CDS             10921..11103
                     /locus_tag="VW854_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEV0262384.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            11240..12613
                     /gene="mnmE"
                     /locus_tag="VW854_001143"
     CDS             11240..12613
                     /gene="mnmE"
                     /locus_tag="VW854_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEV0262385.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNIRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEANLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLIEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(12660..13595)
                     /gene="dapA"
                     /locus_tag="VW854_001144"
     CDS             complement(12660..13595)
                     /gene="dapA"
                     /locus_tag="VW854_001144"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEV0262386.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKTIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(13651..14727)
                     /locus_tag="VW854_001145"
     CDS             complement(13651..14727)
                     /locus_tag="VW854_001145"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEV0262387.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGSSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPCDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(15069..15141)
                     /locus_tag="VW854_001146"
     tRNA            complement(15069..15141)
                     /locus_tag="VW854_001146"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15106..15108),aa:Thr,seq:ggt)
     gene            complement(15241..15750)
                     /locus_tag="VW854_001147"
     CDS             complement(15241..15750)
                     /locus_tag="VW854_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEV0262388.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(15748..15844)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            15873..16304
                     /locus_tag="VW854_001148"
     CDS             15873..16304
                     /locus_tag="VW854_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEV0262389.1"
                     /translation="MKIPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            16386..16763
                     /gene="mscL"
                     /locus_tag="VW854_001149"
     CDS             16386..16763
                     /gene="mscL"
                     /locus_tag="VW854_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEV0262390.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(16805..17899)
                     /gene="hemH"
                     /locus_tag="VW854_001150"
     CDS             complement(16805..17899)
                     /gene="hemH"
                     /locus_tag="VW854_001150"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEV0262391.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGMRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENIKMPYLIKKMLEKAGKLPKE"
     gene            18053..19273
                     /gene="pepT"
                     /locus_tag="VW854_001151"
     CDS             18053..19273
                     /gene="pepT"
                     /locus_tag="VW854_001151"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEV0262392.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTSLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEA
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSNRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVRSL"
     gene            complement(19316..19945)
                     /locus_tag="VW854_001152"
     CDS             complement(19316..19945)
                     /locus_tag="VW854_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262393.1"
                     /translation="MNKANQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDPQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSAKSKDKTALLESTKAILVLNHQIQSKASASEETSPARNAEANGDNTSAENQP
                     NATAESNIETASDENKPSNTRDSKPAESTSENKTTESSTTTGNQEKPVE"
     gene            19910..20131
                     /locus_tag="VW854_001153"
     CDS             19910..20131
                     /locus_tag="VW854_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262394.1"
                     /translation="MDLFQELICLVQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            complement(20240..20485)
                     /locus_tag="VW854_001154"
     CDS             complement(20240..20485)
                     /locus_tag="VW854_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV0262395.1"
                     /translation="MVMVFDANRIISEDSEGFVIPHGDHNHYIKVQTKGYEAALKNKI
                     PSLQSNYQPGTFDEKAVLAKVDQLLADSRSIYKDRLS"
     gene            complement(20592..20687)
                     /locus_tag="VW854_001155"
                     /pseudo
     CDS             complement(20592..20687)
                     /locus_tag="VW854_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(20757..20897)
                     /locus_tag="VW854_001156"
     CDS             complement(20757..20897)
                     /locus_tag="VW854_001156"
                     /inference="COORDINATES: protein motif:HMM:NF016182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV0262396.1"
                     /translation="MLEITLKSPYQFAHILFQSTIVPHGGHYHFIPESDLSAGELAVA
                     KV"
     gene            complement(20941..21501)
                     /locus_tag="VW854_001157"
                     /pseudo
     CDS             complement(20941..21501)
                     /locus_tag="VW854_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(21727..24846)
                     /gene="phtE"
                     /locus_tag="VW854_001158"
     CDS             complement(21727..24846)
                     /gene="phtE"
                     /locus_tag="VW854_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEV0262397.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKIDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEEVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQQSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNSSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGISHEKHEEDGYGFDANRII
                     AEDESGFIMSHGNHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPGN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
     gene            complement(25056..>25478)
                     /locus_tag="VW854_001159"
     CDS             complement(25056..>25478)
                     /locus_tag="VW854_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEV0262398.1"
                     /translation="VRKNKADQDSKPDEDKGHDEVSEPTHPESDEKENHAGLNPSADN
                     LYKPSTDTEETEEEAEDTTDEAEIPQVENSVINAKIADAEALLEKVTDPSIRQNAMET
                     LTGLKSSLLLGTKDNNTISAEVDSLLALLKESQPAPIQ"
BASE COUNT         7306 a         5235 c         4605 g         8332 t
ORIGIN      
        1 aaataccaac catctggctt atctgaatat cccttgaagc ctaattgatt aaaagtagtt
       61 atttgaccag taccttgatt gatgttttct tcaacttcat taaagcctat acttctcttg
      121 cataatccct aacttgatct tctgtcctta aagtcatttt ctattcctcg tcactaaaat
      181 tatcttcaaa actaataata tcattaggag tgcaagataa agccttacat attctttcta
      241 aattcttaaa tgtaattggt ttatcctttc ccatttgtgc cataacattt gttgtaagtc
      301 ctgccatagc tattacatct gttttcttta gtcctttttt tgctaactgt atccataatg
      361 gtttatagtt aagtgccata atatccctct ttttttcttc attaaattta tttaattata
      421 gcataaatat tgatttcatt caatcttaca ttgatttagt gatttgtttt agagaatttg
      481 ttgtgttatt ccgtccttat tttgccataa attgttttaa gtgggacctc ttatccttac
      541 catttttacc tcctaatttt gagtataaaa aagacgatag agtttttaat ttctatcgtc
      601 tgagatttac atattaaatt ttcatcacaa aataagactc tttaaagaat ctttaaattc
      661 attggtatgc tgcttacaat atccattatt aaaatggata ttgtaagcag cacaccaact
      721 atttattttt tgagttttaa actttggtag aagtcttgaa ctccttatat ttacataact
      781 atttgcctct ataactgtta tgacacgaaa ccaacaagca agttcaagca cctccatatc
      841 tttattcaaa tgaggactaa gatattcaca aactttatca taaacctctt tatctttttt
      901 cataaaaggg gcatacaact tagcaaattt atcctaaaat tttttgttgc ctttatcaat
      961 catttcaacg ctcctttaaa tataaaaatg atcctatcaa tctttatatt gagcctattt
     1021 tctaattgat accttgcaag aaaggaataa tagctgttcc tataccaagt aggaatgtaa
     1081 taaatgcaga aagtcatata ctgtcattct tttctattaa atcattttga agtgtctcaa
     1141 agcatccatg atggcttctt ctttttcagg tgtacactgt ggaataattt gtttatcctt
     1201 tttagacttg ttgtaatgtt ctcgtaattc tattccacat ttctttttta tctgtgcaat
     1261 atataatgtc gaaactttta attcaaattt attccaaaca tattctttga tttgagcata
     1321 tgttgctttg ctctccgcac ttgtcaaatc catctcatcc agctcaattt caacacttat
     1381 gtgcttatcg acatcgagtt tggacaaaag tgctaccgtc tcgacgtgat gcgtttgagg
     1441 aaatagatcc accggctgga ctttcttcaa ttcatatccc aactcttggt atagtttaat
     1501 atcacgcgcc atggttgcga cattgcagga gatataggcg atgcgatcgg ctcctgtttg
     1561 ggcgcttgct ttgataaagc tttctgtcaa gcccttgcgt ggaggatcaa ccaagataac
     1621 ggttggttga atcccatctt taagccaatt cttcatagca ttttcagctg tgtcacagac
     1681 atagtgggcg tttgaaatat tgttcagctg agcatttttt ttactattct caaccgcttc
     1741 tggaatcagt tcaacaccgt agacttcttt gacatgcttg gcgactgata aaccaatggt
     1801 tccaatacca gaataagcat caataaccac gtcatctttt tttaactctg caaagtcaat
     1861 ggctgtttga tagagtttct ccgccatttc agtattgact tggtaaaagg ctaggccagc
     1921 gatttggaag tcatttccca acatctggtc cgtaatatag tcttgaccat aaagagtgcg
     1981 ccactcctta ccaaaaatcg cattggtatt ctggtcgttg atattttgca tgacagacac
     2041 aatctctggg aactgcttga taacttgttc aatcaattgg tcaacacgaa aaacttttgg
     2101 acgagttgtc accaaaacga ccatgatttg tcctgaatag tgaccacgac gcaccacaag
     2161 attccgaatc aatccagact gttccttttc gtcataaggt tttaaatcaa aacgacggag
     2221 caggtctcga agagctacta cgacttggtc aatgacagga tcctggataa agaaatcttc
     2281 aaggggcatg aggttgtgcg aattcttacg gaaaaatcct gtttccaaga caccattcac
     2341 tcgacgaacg ggcacctgcg ccttattgcg atacttgact ggatgttcca taccaagcgt
     2401 ttcagcaact tctacatctg caattccagc aatcttgtag agactgtcct tgacttgctt
     2461 ggttttaaac ttgagctgtt ctggataaga aaggtgtcct aaatccgcga ttcctgaacg
     2521 caggtaagcc aaatctagat cttgattacg gtgtggtgac tggacaaggt atttttcaac
     2581 ttttccaaag ccaatctttt tattgacctt gaggacacgc atgagaattt tttcactcgg
     2641 taaagcattc tctacaaaaa agaccaaacc atctaccttg gcaactcctg ccccttcatg
     2701 ggtcaaatca acaatttcaa cttctacaat atcatttttc tttaacattc ttttctcttt
     2761 ctattggccg acaaaaaaga cggcaacgtt acggttacca tccattatac catgaaattt
     2821 cttaagaacc aaaactcttg aagaagttga caagggcttg ccagagggag cttaagaagt
     2881 taccaccgtc ctctagcgcc ttatccgaat caaagttaag gttgatattt ttaaaactgt
     2941 cgccagcttg tgatacgatg ctttgtttaa ggtcatttaa ggttttagtg aaatctgcat
     3001 tgctgaggat atcactcttt gagagattca aggcaaaatt gatgatgata ttgatctggt
     3061 ttcctgttat gacctgatca agtttgtaat tttttaaggt atcttcaaca atcttgcgga
     3121 tatcttcttc tgtcagattt cccttgcttt ctttagcttt ggcgagtcct gacttgatat
     3181 cagctagggc aacgtttaat ttattagcat catagcctga tttgtccttg ttttcagcat
     3241 tgatatctga caaagccttt agctcttctt gagccaaatc tttattggct tgcggcacct
     3301 tggctccatt agcttctagc gaatagtaga tcccagccaa agcactctca cctgtaactg
     3361 gaatgggtgc tgctacagtg attttggcgt gttccacacc caaagttacg gctgcatttc
     3421 ggtacatatc ctgagttacc ttagtgatgt tttcaggtgt ttcaatcttg acctcaagtg
     3481 gcgatttgtc acctagcttt tgaatcttgg ctgatgaata caactgtaag ctagagtcat
     3541 tggccacatt catgatttta gaataaacat caggtgtcat ggtcttgagt tctttggtat
     3601 ctgttgaggc attgtagccc agttttttaa gagtttgatt tttttggtct tcagataggg
     3661 aagaacctag gacatattca ggttggacat aggtttcatc gataactttt tgaacatctg
     3721 ttgctgcatg gacgctattc atagctgtta ctgcccacaa gatcgcagcg ctagtcagaa
     3781 agagtttctt tctcataggg aatttcctcc tttacttctt tagagtaata tatctatctt
     3841 aaagaaaact tataataaaa acacctggtc tagccagatg ttgaaaagag agtgaaacat
     3901 ttgatgatgt aaaggttaag tcgtacctgt ctagaataat aatagtttcc tccatttaca
     3961 tagagttcag caccgtgaaa aatggaaatg gggtgaatat aactataagt ctttccagtc
     4021 ctattaccaa gcaagggggc aacagtctca cgagagtact gtttggctag agccaaggta
     4081 ttttccttgc cattttgggc gataaaatcg atataggcag gtccaaaatt ataggcttga
     4141 acagctgtcc agatatctac acccttcttc tgcgccagat agagattgcc tgtcagagtt
     4201 tgaatgcctt gccgaatgct agaggcatta tcattgatgg tgttggtgga accacttgca
     4261 gactcactag actgcataac atcgccttct tttccttttg tttcagtata aatcatagca
     4321 agcacaagct cttcgtttgc tggggtgtct ttttcactca atatttctcg caccatgggt
     4381 tgataggtca tgacttgttt gacatcttga tgaacgcggt aagctttata gccagcaaaa
     4441 aggaagactg ctagtacaag cactcttcga attcgtttaa acattattta ctttggatat
     4501 cctcgatatt tttgattaag atagagtagg ttccattttc gttttggata aactcaacag
     4561 actcggcgtc ttgatagacg ttattgggaa cgatgagctc aattccattt gataaggaga
     4621 gtttttggtt ttcaaatttc tttaattggc gactggcatc aatttcatca aattgaacag
     4681 gttctggtac ggcttctttg acttggtcaa taaagctcaa acgagccgtc agattgttgt
     4741 caaaaaggtc attagccaat ttctcaggtg acaattcatt gctttcttct aggttgttga
     4801 aaatagctga tttgaccttg gattgaaatt gaaaatcatc tgtgttaaaa gattcagcaa
     4861 ttctctgggc tgttttttcc agttccttga tagatttttt aggagaaatc ttaggagcga
     4921 cagcaagaag attatctgaa aaatagttca aaaaagtccc gttgtacttg attcgttttt
     4981 caatcaggtg atacttgcga ctctgaagat tgaccaccaa ggcctcgtca gcacccgttc
     5041 caaatccagg caggttattc tgagtcagct tgattggatt atcaacttct cctccgaggt
     5101 gggtcaaggt ctcccgcagg gcaattcgca agaaagcgaa atgttctaca ccttctttag
     5161 aaaattgaac aaaaatcaag tcattggtct tgagattttc agaaatgcta aactcctctt
     5221 tccagagatt agccagcgtt actgatgtct ccaacaaatc gtctgtaata tgattgaaga
     5281 agggattttc ttcttcgaaa atcccagtct tggcttcatc tgaatacaca tgttcaattt
     5341 ttttacgtag gtattcttcg atttttggag taatattgag aaacttatct gctaagaaca
     5401 gctcggtatc atccggactg aactggtgaa taatggcttt cttaatataa atgtccataa
     5461 aagttttatt cctcgtataa tgggaaggca tctgtcaatt ctttgactgc acttctcact
     5521 tcttctaata cagcctcatt ttctgaattc ttaagggttt taatgatgag ttcagccact
     5581 ttgcgacttt cttcttcacc aaatccacgt gcagtaatag ctgctgctcc gatacgaatc
     5641 ccacttgtct tgaatggtga caaggtttcg taagggattg aatttttatt tagggtaata
     5701 ttgacttcat ccagcaagtt ttgagcaact tttccgtttt ctacaacctt agtcacatca
     5761 acaaggaaga gatggttttc agttccacct gaaataatac ggaaatcagg gtcttgcaag
     5821 aagacatctg ccatagcctt gctgttctta attacattgg cagcatattc cttgaaggct
     5881 ggatccaaaa cttctttgaa ggaaactgcc ttagccgcca caacatgctc taaaggaccg
     5941 ccctgaatac ctgggaaaat agctgaattg atttttttag caagttcttc gtcattggtc
     6001 aaaatcaaac caccacgagg tccacgaagg gttttgtggg tcgttgttgt tgtgatatga
     6061 gcgtatggaa ctgggcttgg atgaaggcca gccgcaacca agccagcgat atgggccatg
     6121 tccaccatga gcttcgcacc gacagcatct gcgatttcac ggaattttga aaaatcgata
     6181 atttgagaat aggctgaagc accagctaca atcagttttg gttttacttc ttgggcttgt
     6241 ttcaagatag catcaaagtc taagagttcc gttttaggat caacactata agaaacaaag
     6301 ttgtaggttt gaccagagaa gctaacagga gccccatgag tcaaatgacc acctgatgcc
     6361 aaatccattc ccataaccgt atcacctggc tcaatcaagg acatgtaagc cgcacagtta
     6421 gcttggcttc ctgaatgtgg ttgaacattg gcaaatttag caccgaaaat ttcttttgcg
     6481 cgttcaatag caagagtctc tacaacgtct actacatcag ttccaccata ataacggcgt
     6541 cctgggtaac cctcggcata tttgtttgtc aagatagacc cttgagctgc cataacagcc
     6601 ttggaaacta cgttttccga agcaatcaac tcgatattat tttgttggcg ttcttcttct
     6661 ttagcaatag cattccagag atcagcatca tatgctttaa aatcatcttt gtcaaaaatc
     6721 ataggtcttc tcctttattg tgtgactagt ccattagttt gattttacaa taagaaaata
     6781 aactaaaaga tgcgaataaa ccgtttctgc actttatcac aagtataacc aactttttca
     6841 taaaatgcat gagcacccag acgatgattg gcagaattta agcggataaa cccataacca
     6901 catcttttgg cttcttgttc caacccttgt agtaaacttt taccgatacc ttgaccttgc
     6961 gcttgaggtg aaactgctaa agctaagata ttaaatcctg ctttggaata gagtgattcg
     7021 taaacttcag cgtggacata tccaagtaag acatgattag ctgcatcctc atagccaagt
     7081 aggaaatgat gggaatcctg agacagtcta gctagttggc tagccgtttc ctctggacta
     7141 aaagtataac ccaaagcctc ttggttgatg tcacatatcg ctttcacatc tgtttcttgc
     7201 aaatctctta gcatttcaag cctcctcaaa agaaatctct ggcaaccgag caagaatatc
     7261 ttctcgttta attgcgcctt ggcgtaagat tttcaccttg tctccagaca aatccacaat
     7321 agttgaatcc tgtccagtta gaaaagcatc gtcttccaga cccagaacct cttggtcaaa
     7381 atccttcaga atttgttcaa aggttacacc acttgcctga cctgagatat tggcagacgg
     7441 cccaatcaag ggacctgtct ctcgaattaa atccagtgtg atagggtgac tgggcatccg
     7501 aaatccaata gttgcaaggt cagaatttac ccaataggga actcggtcat tggcttcgag
     7561 aataatggtc aagggacctg gcaaaaaggt ctctacaagt ttttgtagat aagctggctg
     7621 attctttgaa aagtgcaaga tgtcctcgaa agaggcgata ttgagattga gtgccttatc
     7681 tctaggacga cgtttgagtt ggtaaacatg gtcaactgct ttttcatcta aggccttgga
     7741 aaaaagacca taaacagtct ctgtaggtag aacgacagct ccaccatttt ccaactcttg
     7801 tctaatcctg tccatcatca accacaacca tcctattttg accaaattgg tctttgagtg
     7861 ttcgaactct tttttcagga agatgttttc taaaaagttc gggaacactt tgaccttgct
     7921 tgtatccaat ttcaaggtaa atcttaccac catctttgag atagtctttt gcatcttccg
     7981 caattctaca gtaaatagct aggccatcct cgtctgcaaa gagagctaga tgaggctccg
     8041 aatacaagac attcaagcct acctctgact catcttcacg agagatatag ggtggattgg
     8101 aaacaattat atcatatttt tcagaaattt ctgtaaaaca gtcagatttt ttaaaaaata
     8161 tttgaagatt ttgattttta gcattttcgc tagctaaatc taaagcctct tgagaaacat
     8221 ctgctgctgt cactgaccaa tctggtctat tttttgctag agcgagagca atagctccac
     8281 tacctgttcc aatatccaga actgaaagat tcgtctcaag attttcagtt agaataagct
     8341 ccactaactc ctctgtttct ggacgaggaa tcaaaacccg ttcatccacc tttaaatgca
     8401 ttccataaaa atctgcctgt ccaatgatgt actgagctgg cttgtgagct gctagttgct
     8461 ggtaaatatc ttctacaaat tgtttttctt cctctgttgt cacctcctgc tggagggcaa
     8521 aaataaagtc tgtaaaagat agatttttca gactacgata gacaaaagag aggctttccg
     8581 cttcctctcc ttgtcttatc aactcttctt caaaatttga aaataattga gctaatttca
     8641 ttatttgttt aattcttcta gtttttgtgt ttggtcataa agcaccaagg catccacaac
     8701 ttcgtccaat ttaccagaca aaatcgtatc tagtttttgg agggtcaagc cgatacggtg
     8761 gtctgtgaca cggttttgtg ggaagttata agttcggatc cgttctgaac ggtcaccagt
     8821 accgattgtc gacttacgct cagcgtcttg ttcatcctga gcaatctgag caaagtggtc
     8881 agcgacgcgt gcacggataa tcttcatagc cttctcgcgg ttcttctgct gggtacgttc
     8941 ttcctgcatc tcaaccttga tattggttgg caagtgaacg atacgaacgg cagtcgcaac
     9001 cttattgacg ttctgtccac cagcaccaga ggcgtgatag atgtcgacac gaaggtcttt
     9061 tggatcaatg tcgtattcaa cctcttcaac ttctggcata acaagaactg tcgctgtcga
     9121 agtatgaaca cggccttggc tttctgtcac aggaacacgt tgcacacggt gggcacctga
     9181 ttcatactta agcttagagt atacagactg acctgaaacc atagcaacca cttctttaaa
     9241 accaccgaca ccattcatag aggcttccat gacttcaaag cgccaacctt gggcttccgc
     9301 atacttttgg tacatagtta gcaaatctcc agcgaaaagt gccgcttcgt ctccaccagc
     9361 tgctccacgg atttcaagga tgatattctt gtcatcgttt ggatcctttg gaaggagcaa
     9421 aattttcagt ttttcttcat attcttcttt ttcagccttg gcatctttga gttcttgctt
     9481 ggccaattct tccaagtccg catctccgcc tgattcctta atcatctctt cggcatcgac
     9541 gatattttga aggacttgtt tatactcacg gtaggctatt acggtgtcac gattggaagc
     9601 ttcttctttt gaaagctcca taaaacgctt ggtgtctgaa acgacatcag ggtcactcag
     9661 caattctcct agttcttcat aacggtcttc tacagcttgt agttgatcat agatgttcat
     9721 tttttctcct tatttctcaa ttgttaaatc atagattgct actacttcat tctcggatat
     9781 ttccccagtt tctttaaatc cataactgag gtaacaaaat cttgcctgtt cattttctgg
     9841 ttcataagac aaccaaagtt tattgcttaa acctgctggc gctgttcgaa catagtctag
     9901 tactttatcc ataattggtt taaaatatcc ttgattttga aaattcttat caatcataaa
     9961 acgaaatagt aaataatttc cactactaat tccgatcttt ttatcataag ctatcatcac
    10021 aaaacctata attgcatcat tatcataaac tgccaatgga gctacaaaat ctccattttt
    10081 agtgtagacg tatgcttcag ctaaactaat tgcgttggtt gcaatgaatt gtttttgata
    10141 ttccttgaca tccaaattta aaacatcaaa ataattttcc attgtaacat ctcttagttc
    10201 aattgtcata gttttgctcc ttgttagagg ttatcattgg cgcaaaataa tgtttacggc
    10261 aaaccgagat ataggtttca ttaccaccaa tctggatctg ttctccatcg taaacgggca
    10321 gtccatcctg tgttcgcaac accatggtcg cctttttctt gcaatactga cagatggtct
    10381 tgatttcgtc aatcttgtct gctaaaagca agagatattt ggaaccttcg aacaattcat
    10441 tgcgaaagtc atttttcaag ccaaaagcca tgacgggtat gtctaactcg tccacaacac
    10501 gagctaggtc ataaacatgg tggcgtttga gaaactgggc ttcatcgacc aaaacacagt
    10561 aaggtttttc tggtaggtct cggatatagc caaagatatc cgttgtttcc tcaatcgcaa
    10621 gggcagggcg tttcatgcca attcgactcg acacatagcc aacaccgtca cgcgtatcca
    10681 gagccgaggt cataatcaca acaccttttc cttgctcctc gtagttatag gccactttga
    10741 gaatctcaat cgttttacca gagttcatgg tcccataacg atagtacaac tgtgccatgt
    10801 ttcttgcttc acgtccattt ctaaattttt gctacattct agtatatcat aattttctta
    10861 agctttaaac ggcaaaatgt ggtaaaatgg aagaaatcaa aactagtgga ggaagctatt
    10921 atgccatttg tacgcatcga tttatttgaa ggacgcacgc tcgagcaaaa gaaagctctt
    10981 gctaaggaag taacggaagc agttgtccgc aacactggag cccctcaatc tgctgtccat
    11041 gtcatcatca acgacatgcc agaaggaact tacttcccac aaggggaaat gcgtactaaa
    11101 taagctagct taagcagaat tgcttaggct ttttcaatct ccaagtagca ttcattgaag
    11161 aaatatccta aatttgttac aatttgaaaa gaaacttgga gaatttccaa gaaaatagct
    11221 attaattaaa ggaaacatta tgattacacg tgaatttgat accatcgctg ctatctctac
    11281 tccactaggt gaaggggcta ttggtattgt ccgcctgagc ggaacagaca gttttgctat
    11341 tgcgcaaaag atttttaaag gaaaagactt gaacaaggtt gccagccaca ctctcaacta
    11401 cggtcacatt attgatcctc tgactggtaa agtcatggac gaggttatgg ttggggctat
    11461 gaagtctcca aagaccttca ctcgtgagga tattatcgag attaacaccc acggtggtat
    11521 tgcggtgacc aatgagattc tccagctagc tatccgtgaa ggagctcggt tggcagaacc
    11581 tggtgaattt accaaacgtg cctttttaaa cggtcgcgta gatttgacac aggccgaggc
    11641 ggtgatggat atcatccgcg ccaagacaga caaggccatg aacatcgcgg tcaaacaact
    11701 tgatggttcc ctttctgatc ttatcaataa tatccgtcaa gaaatcctta acacacttgc
    11761 ccaagttgag gtcaatatcg actatcctga gtatgacgat gttgaggaag ccactactgc
    11821 tgttgtccga gagaagacaa tggagtttga gcaattacta accaagctcc ttaggacagc
    11881 acgtcgtggt aaaatccttc gtgaaggaat ttcaacggct atcattggac gtcccaacgt
    11941 tgggaaatca agccttctca acaacctctt gcgtgaggac aaggctatcg taaccgatat
    12001 cgctgggaca acacgagatg tcatcgaaga gtacgtcaac atcaatggtg tacctctcaa
    12061 attgattgat acagctggta ttcgtgaaac ggatgatata gtggaacaaa tcggtgttga
    12121 gcgttcgaaa aaagccctca aggaagccaa cttggttcta ctagtgctaa atgccagtga
    12181 accactgact gcgcaagaca gacaacttct tgaaattagc caagatacca atcgcattat
    12241 tctacttaat aaaaccgacc tgccagaaac gattgaaact tcgaaactac ctgaagacgt
    12301 tatccgtatt tcagtcctta aaaaccaaaa catcgacaag attgaagagc gaatcaacaa
    12361 cctcttcttt gaaaatgctg gcttgattga gcaagatgct acttacttgt caaacgcccg
    12421 tcacatttcc ctgattgaaa aagcagttga aagcctacaa gccgttaatc aaggtcttga
    12481 gctggggatg ccagttgatt tgcttcaagt tgacttgact cgtacttggg aaatcctcgg
    12541 agaaatcact ggggatgctg ctccagatga actcatcacc caactcttta gccaattctg
    12601 tttaggaaaa taagaaaaat ccatgatcct tcattcggtc atggatttta ttgtctttat
    12661 tagtaatctg gtcttaagac ccctgttaca gttgccttag ttgcttcgta gtcgccatct
    12721 acgacaacct tgataatgcg tttggcatct tcttctggtg ctggaacaag aggtagacga
    12781 gtgggtccag cttcaaatcc catatagtta agaattgcct taactggagc aggacttgga
    12841 taagagaaga gagcattaac cttaggaatg aatttacgct gaattgctgc ggctttcttc
    12901 atatcgcttt ctgcaatggc agtaaacatc tcgtgcattt catccccatt tgtatgagag
    12961 gcaacagaaa taaccccatc cgccccaagg ttcatggcat ggaaagcatc tccatcctca
    13021 cctgtataaa tcaagaactc ttcaggcttg tgctcaatca agtaagccat attagccaag
    13081 ctagtacatt ctttgacacc gataatattt ggatggtcag ccaagcgaag catggtttct
    13141 ggagtcaatt cgacaactac acgccctgga atgttataga taataattgg taggtcagaa
    13201 gcatctgcaa tagtcttaaa gtgctgatac atcccttctt gagaaggttt gttgtagtaa
    13261 ggaacaatag caagcccagc tgcgaaacca ccaaattccg ctacttcttt gacaaactca
    13321 atagagtcac gcgtatcatt agtacctaca cccgcaatca aaggaacgcg tccattgaca
    13381 accttttgta cagccgcaaa caactccaac tcctcatcgt gggtcaaagt tggactctca
    13441 gcagtcgttc ctgcgagaag aattccatcc gtatgatggg ccaataaatg ctcaatcaag
    13501 gctggaatag catcaaagtt aatagaacca tcctcatgaa agggggtaat aaaggctgta
    13561 atgattttac attcttttaa atcttgataa gacatgaaaa cacctctcta tttcaaagaa
    13621 ggagttcatc tgaactccta aacgatatga ctattttaat tcaaatttca attcggctgt
    13681 tggacgaacc aatccacgtt catgaagagt ttcagcaatc tgaactgagt tccaagcagc
    13741 acctttgaga aggttatctg aaacaaccca catgtgaatt cctttttctg catccaagtc
    13801 tttacggata cgaccaacaa aggtatcacg cgaaccaact gcattgatag cttgaggata
    13861 gatttgatga gctacatcat cttcaagaac agcacctggg aaggctgcga tagctgcttt
    13921 tacttcttcg attggagcca cttcttttgt ttcgatataa acagactcag agtgagctga
    13981 caagactgga atacgcacac atgttgcaga tactgcaatg ctatcatctt ccataatttt
    14041 cttagtttcc ttggtcatct tcatctcttc gtaagtgtaa tcattgtcag tgaagacatc
    14101 gatttgtgga agagcattga aggcgatagg ataatgtttc ttgtcaccac ctgaaggcaa
    14161 aatttccgca tgcaaatcac atggtttcac accatcattc aagacctcac gaagttcacg
    14221 ttgtgtctca agaattgctc ccataccagc acctgaaacg gcttgataag ttgaaacaat
    14281 gatacggtcc aagccccatt tttggcgaac cggctcaaga gccaccatca tttggattgt
    14341 tgaacaatta gggcaggcaa tgattccgtt gtgagcatca agtgcatgag cattgacctc
    14401 tggaacaacc aaaggaacat ctggattttg acggaaataa gatgtattat ctactactac
    14461 cacgccagct tttactgcgt atggtgcata cttagctgat gtagaactac ctgctgaaaa
    14521 gagagcaata tcaactcctt caaaagctgt ttcagtcgtt tcttcaattg taatatcttg
    14581 atctttaaat ttcaatgact tacctgctga acgtgcagaa gcaaggtaac ggattttatc
    14641 gattggaagt gttgattctt ccaacatttt tatcatctga gcaccgacag cacctgtcgc
    14701 gccgactaca gcaactgtat atcccataaa taacctcttt cggaattttc taaaaatttc
    14761 tataatagaa gtattatact actttttcca tgaattgcaa agctttttca ttattttttg
    14821 gaaaataaaa atcccagtcg ctagactagg gactaagagg catcttcatg tgatgagcag
    14881 gttcacacaa ctcatcaagg tccgctcctg cgttatgacc tccttatgct caatagtagt
    14941 ccgaagacta cctatcgact catcgcatat tctatattac tagagatact tgattttgtc
    15001 aataagaaat tttttcgatc gagtataaca aaaaactctt gtattcaaga gtttccctat
    15061 acatatcatg ctaattgccg ggattgaacc ggcgacctca tccttaccat ggatgcgctc
    15121 tgccaactga gctaaatcag cttacttaag aagtatacta taatcagaaa aacttgtcaa
    15181 gtttccttag aaattttcca taggaaaaag ccaggtaaga actacctagc ttatcttctt
    15241 ctatttactt aaatcaattc gacgaccaat tttaccaata ataatcgctc caataatcaa
    15301 tgaagcaaat tcaccgattc ctgttgtaaa ccaagtaagg aagaatggta attctgctac
    15361 aatatttaac tctgcagcga tagtaaacat ggaaattgaa aagaggattg aaaagaagaa
    15421 atgatctttt cgaatcaatc cgttaaagag gtaatccttg ctatattttg caaaaagcca
    15481 aacacctaaa ctgaggaata ctaaggttga accaccacca acaaagacat ctagcagtcc
    15541 gaagctaaag aaattagcaa tcatacaacc gatggtaact ccgatgatgt acttaggatt
    15601 gtaaaaagcc ataaagttca tcatttctga aatacgaaac tgataagcac catagctgat
    15661 ggcatttagg ggcggtgtga cagtcaaaac gacgtagata gcagcgacga ttgcaatatc
    15721 tgcaacatca cgaatagtta attttttcat cttttctcct ttggcggtta tccgcgtgta
    15781 atatgcttgg tgaaagaagc taagcaccaa gggttgattc attcaacctt actagtatag
    15841 cacaatagcc tgctttatgc tatacttaaa gcatgaaaat tcctattcaa aaatttttta
    15901 acaatgaaat cttagcctat ctcttttttg gccttgcgac aactctagtt tcgattctct
    15961 cacgactagt catttatcaa ctaagtcatc aggaactcct tgctacggcc ttggcaaata
    16021 ttatcggtat cttatttgcc tttatcacaa atgatacgat tgtatttaag caaaaaaaga
    16081 aaaatagact tatccgttta gtgaaatttt ctcttgctcg cctttctact tttctgttag
    16141 acttgttttt aacttttctc tttgtaaccc aatttcctca catcataggg caattcgtaa
    16201 ctgaaaacat tgataagata aatgctattg aaacaataat tgcacaaata ttcattatta
    16261 tcatcaatta tatcttgagc aaggtctata tttttagaaa ataatgctcc atttatgagc
    16321 cttttaattg ccttctataa tgatttagga tacaatgaaa cacgaaacat tgaaaggaaa
    16381 acagtatgtt aaaaaatcta aaatcgttct tgcttcgagg aaatgttatt gaccttgctg
    16441 tcggtgttgt cattgcctct gcttttggtg ctatcgttac ttcacttgta aacgacatta
    16501 tcactcctct tattttaaat cccgctttga aagctgctaa agtcgaacgt atcgctcaac
    16561 tttcttggca tggagtcggc tatggtaact ttttaagtgc tattatcaat tttatctttg
    16621 tgggtaccgc cctcttcttt attatcaagg gcattgaaaa agcacagaaa ctgactggca
    16681 taaagaaaga aaaaactgcc gaaaaaaaac caaccgaatt ggaagtcctt caagaaataa
    16741 aagctctcct tgagaaaaaa taatcgatta aaaggatcta gcgcaaagct aaatcctttt
    16801 ttctttactc tttgggtaac ttgcctgctt tttcaagcat ttttttgata agataaggca
    16861 tcttaatatt ctcccgccct tttttctcaa tcagtttttt cacaaattct ggcatttgta
    16921 aatcttcaga ttcagctaaa atgtttttcg tctcgtctga aggaactaac tcatcaaagg
    16981 tctcttcttc tgggagaaat tccttaaact cttgattttc attgactcta actgtattga
    17041 ccaaagcatc aatcaaacga gaatctgtat ttggcattgg tggacgatgg tagtttaccc
    17101 caaattcctg acataaatca taacattcca catcattgtc aaacaagacc tcaatatgct
    17161 cactgatgaa actaataggt acaaaaatat aatggtctgg atgttctgtc tgttctctga
    17221 gatactccaa aacatctggt ttaatccatg gaataccaat atcactctca ctctgccaag
    17281 tgttggtata ttgttctgaa ctcaagccca gtttttcagc gactagcttg ctattttcga
    17341 aaatttggtc tatataagga tctccaaagt ccaaggcaaa aataggcaca ctatgggctg
    17401 aaaagatgac tttaaagctg tcttgcttta cttcttcttt taaaatctta gcaatttcat
    17461 ctgcccaata gtttaagagc gcttcttcct gataccagtc cttaatgacc aaaaattgaa
    17521 tttgtttgct ttctaaaaat ttttcatagc ccatgacaga gtagaaagaa taatggggct
    17581 ccaaaatcaa gcaaatacac tgttcaattc cgtctgcttc catttgaccg atcacatctg
    17641 gaataaaggg actggaaaac ttattagcaa agtagacact gtattcattc cccagcctag
    17701 cttctaccaa ggcaacctct tgacgggtaa ttttttgcag aggcgtgcct ccaattcgta
    17761 cataattatc atagagagtc tgaatctcgt ggtcttgggg tctcatccca cgacgaatat
    17821 ttgtgaaaaa atcagctaca ccttcaaagg taatctcttc tggtgaaccg aaggtcatca
    17881 ttaaaattgc ttttttcatg actgcttcct tgtgatgttt ttatcaagtt tattttatca
    17941 ttaattcatc gataagtaaa cgctagcata gggacattct caatcttctg tcttttgttt
    18001 ttctcttctt tctatgatac aatggaaaaa ataaattcaa aaggagtttt ttatgactta
    18061 tccaaatctc ttggaccgct tcttaaccta cgttaaggtc aacacgcgct ctgatgaaca
    18121 ctctactact actccaagta cacagagtca ggttgacttc gcaacaaatg tcctaattcc
    18181 tgaaatgaaa cgtgttggac tgcaaaatgt ttactatcta ccgaatggtt ttgctattgg
    18241 aaccttgcca gccaacgatc cgtctttaac acgtaagatt ggttttatat cgcacatgga
    18301 tactgctgat tttaatgctg aaggagtcaa tccacaggta attgaaaact acgatggtgg
    18361 tgtgattgaa ctagggaatt ctggtttcaa actcgatcca gctgacttca agagtcttga
    18421 aaaatatcca ggacaaacgc tcatcacaac agatggaaca agcttgctag gtgctgatga
    18481 caagtcagga attgctgaaa ttatgacagc cattgaatat ctaactgctc atcctgaaat
    18541 taagcactgt gagattcgtg ttggttttgg tccagatgaa gaaatcggtg ttggtgccaa
    18601 taaatttgat gcagaagatt ttgatgtgga ttttgcctac actgttgatg gtggtccact
    18661 aggtgaactt cagtacgaga ctttctcagc cgctggtgct gaattgcatt tccaaggccg
    18721 taatgtccac cctggtactg ccaaagggca gatggtcaat gcccttcagc tagcaattga
    18781 ttttcataat caacttccag aaaatgaccg acctgagtta actgaaggtt accaaggttt
    18841 ttaccatcta atggatgtga caggtagtgt tgaggaggcg cgtgcaagct acatcattcg
    18901 tgattttgaa aaagatgcct ttgaagcgcg taaagcatcc atgcaatcta tcgctgataa
    18961 gatgaatgaa gaacttggga gcaaccgtgt cactctcaac ttgacagacc agtactacaa
    19021 tatgaaagaa gtcattgaaa aagatatgac tccaattacc attgctaaag ccgttatgga
    19081 agatctaggt atcacgccta ttatcgaacc aatccgaggt ggaacagacg gctctaagat
    19141 ttcctttatg ggaatcccaa ctccgaatat ctttgcaggt ggcgaaaata tgcacggacg
    19201 ttttgaatac gttagccttc agactatgga acgtgcagtt gataccatca ttggcatcgt
    19261 acgtagctta taaaagctaa aaagacgagg tagctcagct acttcgcctt tctttttatt
    19321 ctactggttt ttcttgattt ccagtagttg tagaagattc tgttgtttta ttttctgaag
    19381 ttgattcagc aggtttagaa tctcttgtat tgcttggttt gttttcgtcg ctagcagttt
    19441 caatgttaga ttctgcagtt gcgtttggtt ggttctcagc actggtgtta tcaccatttg
    19501 cttcagcatt tcttgctgga cttgtttctt cacttgcgct agcttttgac tggatttgat
    19561 gattcaaaac tagaatagct tttgtcgatt caagtaaagc tgttttgtct ttactcttag
    19621 cagaaagttg atctaataat gcatccacct tatcaaagtc cgcatcagat ccattattac
    19681 tttctaaata agagtgaagc gacatgagaa tatcgtagag tttttgatag agtacaagtg
    19741 tctgaggatc ttgctcagca ttttcctttt cttgttgaag ggcgctagcg atacgagtca
    19801 aaacatcttt tacctgactg tttacttcat ccaagtctgc atcagccttg tttgtggcag
    19861 cttttagatt ttctacttct tctgccaagg attgtctgat tccttcttca tggatttgtt
    19921 ccaagagttg atttgccttg ttcaaaagac tttctacttc ttccttgcta tctgtcgcag
    19981 attattggtt gctatctacc atgtactcct aaaacaggag agttataatc caagattaca
    20041 aggccttaca gaaataagaa atccagataa gacaatgttc gtccaagacg ctattcgctt
    20101 cgcacagcag cacggattca atatgcttta attttaaagt ttaggtgtca agacctcttt
    20161 ttagtgtgcc caaaatttag agaagtaatc aatcaactaa cttttatttt tttcaaactt
    20221 tcagtaaact gacctaaagc taactcaatc tgtctttgta gatgcttctg ctatcagcta
    20281 gaagttgatc tacttttgcc aagactgcct tctcatcaaa agttccaggt tgatagttgg
    20341 attgcaggga tggaatcttg tttttcaaag ccgcttcata tcccttagtt tgaaccttga
    20401 tgtagtgatt gtggtcgcca tgaggaatca caaaaccttc tgaatcttca cttataattc
    20461 gattggcatc aaaaaccatg accatcttct tcctcatggt ggacatgtag tgacggatta
    20521 cttaatacag aactagaaga acttcctacc tcttccgtgt tagagtgtga tgggggattg
    20581 ttaagagatg acttaggaat atagtgatag tgttccccat gtcttactat ataagcatca
    20641 cctatatctc tgacaatatc attagggtta aagacataac catcatctac tgcagaaaca
    20701 ccattattcg gtgtcaccgg caaagattga ctgagagctg tagtattccc tgataattat
    20761 acttttgcga ctgctaattc acctgccgac aagtcactct caggaatgaa atgatagtga
    20821 ccaccatgtg gtactatcgt agattgaaat agaatatgag caaattgata aggggatttt
    20881 aaagtaattt ctaacaatga tttagaaact atgatgtgct attctaaatt caactcacta
    20941 tatataacca tcatcggtag tataacgtcc ctgtaatttt gctacagata cttctgcact
    21001 agctccttta tcgtctttac catgttcttg tttttggcga ttgatttcat cttttgttcg
    21061 tacattttct gcatgagctt gatctttaag gtaaacataa tactttccat ctcccttaat
    21121 aatatatcct cccttaacct aactgacgat atcttgatct ttcggctgat agttgggggc
    21181 tttcattagt agctcttcac taaagagcgc atcaaaagga actttaccat tatagtagtg
    21241 ataatgatcg ccatgagaag ttacataacc ttgatctgta atcttaataa caatttgttt
    21301 tgcttgaatt ccttcttttt gactaaccta gtctggagtc aaattttcag tcttcttagt
    21361 gtctttatta ctgtttacat atgaaacacg atttttatct gtattggcct gttagctatg
    21421 ttggttcaga gcataaacac acagacttaa ggaaaggata acaacagatc cagctgctat
    21481 atatttcttt ttaaatttca taattacctc atttctataa ttatttatat gatgtcttca
    21541 ttattaaatg attaaataaa ttaattaacc agttaattaa ttagtaaata ttccacctct
    21601 ttttaagttg tatgtcaaga aattttatat attaataata aaatgaaatt ctcccaaagt
    21661 cagagtttta tttctaactt ttgagagaac ttcatttttg attcagactt tttctactgc
    21721 tattccttac gctatgagat cagataaatt cttttttatc acttctccac ttggcaatct
    21781 taattcaatc gttccatcca tattgaatat aacactatct aagcctaatc cgtaactagc
    21841 tgtaaatttt tctaattttt cttgtacagg atctactgct ggagcttcct ctaatgctgg
    21901 atctaacata gggtcactcc ccacattccc ttctggattc aacattccat tatccgttga
    21961 gttttctggt tttacaggtt tttcgtttgg tgcctctggt aaagaatctg ctggtttatt
    22021 ttctgttggt tggttctcaa ctgttccagt agatactttt ccattttcag atggtttatt
    22081 ttcaccattt ccttgaggtg cttctcctgt aaaatctgcc atattctttt taatgacttc
    22141 tcccgatggt aaatataatt caattgttcc gtccatatta aacaagacat tttctagctt
    22201 catcccataa ctttcagcaa attttgctac tttttcttgt acaggatcca ctgtaggaac
    22261 ttcttctaac gttgaattac tagtactatt cccagtttca gaaagttttt ctttttctac
    22321 cttctcacta gtctttggtt cttctacctt ttcatcaagt tttgagtttt cttgtgcttt
    22381 attcctttta aattgtggta gaatacttgg tttatcagtt tgattttctt tttccaagat
    22441 aggtacttcc acaatataag tcgattgatt gtccaaataa gcatttgcca tgaaggttac
    22501 aggaatttta tttccggccg ttctggttgt tccttggttt aatttcggaa tcggtaattt
    22561 gatttcacca actttatagt tattttctaa ataagcattt ccatgaaatt catcaaacac
    22621 tctgactaaa gcatcagttc ctttaggcac tgcaaattga gggttcactc ttaaataagt
    22681 atcccctgca tggaaaggat agaaaatcgt ttgactggcc attttgtaag ctaaagaggt
    22741 tggaactgta aatgtaccat cataacttac ttctggataa tcttttgaag cgatagtata
    22801 cttaaatgtt tgtcctggta aataaggctg atctaattca aagtttgcaa tattccctac
    22861 tccttctcca aatactttac cagatacttt ctccaatact tttccatctg gtgttattaa
    22921 ttttactagc atattgatac ctaatttttt ctccaattca ggcggaaaac taaaagaaac
    22981 gcgtttttga ccattggcta gagtaaagtt ttgattatta aacgtactat tttttaacaa
    23041 attaacaaca ttcgttaatt cttctccagt ataaacttta ttcccttctt ttttagcaac
    23101 tccttcttcg ggtttaaaca gttcatagtt actgtgagaa tgaccaattc caaccggttt
    23161 atgttcatca atcggatctg catgatgatg atctccatgc ggataaataa tcgcattttt
    23221 ttctttattc acgacaatac tttcacgttt gacaccatat tgtttcataa tgccagcaat
    23281 tttttcttcg atttttttat ctaaatcttt catttctttg gcattacctg gataatcctg
    23341 ttcatgagat gacaaagaat ctaatccatt atgactagtt ttaacttcct ctaaatgttt
    23401 ttgcgcagct ttaatttgct cttctgtcaa gtccttcttg aagaagtaat gattatgatt
    23461 accgtgactc atgataaatc ctgattcatc ttcagcgata atacgattag catcaaatcc
    23521 gtatccatct tcttcatgtt tctcatgtga aattcctgga ttgattggaa gagatggaga
    23581 aggtgttgct agactattgt ttggaagagt cggttgccca atttgatttg attttggaat
    23641 gtaatggaaa tgatcaccat gtcttacaat ataagctgta gccgtttctt caacgatatc
    23701 ttttggatta aaaatataac catcagatgc tgaagagagc tccttacttg tcgttaaaga
    23761 agaagaattg cttgaaagac tgcctagact agacactact tcattaggtt ttgcatttgt
    23821 agaaactgta gaaccagttc cactgatagg caccattctg gcaatctttt cttctaaggc
    23881 agaaagcttg ctgtaaggaa taaagtggta atggtcgcca tgcggaatcg caactccatt
    23941 tggtgtacga ctgataatct tagcagggtc aaagaccagg ccatctgatt cactgtaacg
    24001 ttgggcgcta ggtgaatcat agagttcctt caaaagactc tggagatttt cagatttatt
    24061 tgctggcttg ctagttgatc cttttgctac agattgcgtg ttattgtcac tagctgttga
    24121 agaatagctt aactgactct gttgcatatt ttttccagcc agatgtgctt tagctgctgc
    24181 taattcacta gcagataaat cgcttttggg aatgtagtga tagtgacctc catgaggaac
    24241 gatataagca ttacccgtat cttcgataat atcagctgga ttaaagacat aaccatcatt
    24301 tgtcgtatat cgtccctgag accttgctac agcaacatta gagttaacct cctcattatc
    24361 tttgacatgt tcttgttttt gacgattgat ttcatcttta gttcgaacat tatcagcatg
    24421 agctgcatct ttcaggtaga cataatattt tccatcgatc ttgatgatat aaccaccctt
    24481 gacttcattg acaatatcag cgtctttaag ttgatagttt ggatccttca tcaagagttc
    24541 ttcactaaag agggcatcat aaggaacttt cccattatag taatgatagt ggtcaccgtg
    24601 tgacgttaca tagccctgat ctgtaatttt gattacaatt tgctcagcct gaattccttc
    24661 tttctggcta acctggtctg gtgtcaagtt ttcacttttc tgacttgact ggctgccatc
    24721 cacataagag acacgattat tgtccttatt ttcctgcgaa cgatgctggt ttagtgcata
    24781 ggcacataga ctcaaggata cgataacagc tgatccagct gctatatatt ttttactaaa
    24841 tttcataaat ccctcatttc aataaatgat gaagtttttt ctcaacttct tttactttat
    24901 taaatagttt tctaaaccgg ttatttaacc atttaattaa ccagtaaata gtttactatt
    24961 taaaattact tttgtcaagt ttctgttacg tttttctaaa aaagagaagt aacacgaaag
    25021 gttacttctc cataaaatat gctccattca ttttactact gtataggagc cggttgactt
    25081 tcttttaaca aagccaagag actatctact tctgctgaaa tagtgttatt atctttcgtt
    25141 ccgagaagaa gactactttt tagaccagtc aatgtctcca tagcattttg tctaatacta
    25201 ggatctgtta ctttttctag caaggcctcc gcatctgcta tcttagcgtt aataacagaa
    25261 ttctctactt gaggaatttc agcctcatct gtggtatctt cagcttcttc ctctgtctct
    25321 tccgtatcag tgcttggttt ataaagatta tctgctgaag gatttaaacc agcgtgattc
    25381 tctttttcat cagattcagg gtgagttggc tcacttactt catcatgtcc cttatcttca
    25441 tcaggtttac tgtcttggtc tgccttattt ttacgaac
//
LOCUS       DAVKWZ010000027        25443 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000027 DAVKWZ010000000
VERSION     DAVKWZ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25443
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.027"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            272..2967
                     /locus_tag="VW854_001160"
                     /pseudo
     CDS             272..2967
                     /locus_tag="VW854_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268095.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            3268..4107
                     /locus_tag="VW854_001161"
     CDS             3268..4107
                     /locus_tag="VW854_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEV0262399.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKIGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGVIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVEN
                     TTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTK
                     NLD"
     gene            4125..5963
                     /locus_tag="VW854_001162"
     CDS             4125..5963
                     /locus_tag="VW854_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV0262400.1"
                     /translation="MSYKDKVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGVPVIPASYGSSVVPILIMV
                     WLMKYIEKMIAKLTPAVTKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     DVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            5976..7391
                     /locus_tag="VW854_001163"
     CDS             5976..7391
                     /locus_tag="VW854_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV0262401.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEN
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDEFNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            7873..8919
                     /gene="pheS"
                     /locus_tag="VW854_001164"
     CDS             7873..8919
                     /gene="pheS"
                     /locus_tag="VW854_001164"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEV0262402.1"
                     /translation="MSTIEEQLKALREETLTSLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            8919..9428
                     /locus_tag="VW854_001165"
     CDS             8919..9428
                     /locus_tag="VW854_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0262403.1"
                     /translation="MIRKVEVADVEVLAKIAKQTFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            9505..11910
                     /locus_tag="VW854_001166"
     CDS             9505..11910
                     /locus_tag="VW854_001166"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEV0262404.1"
                     /translation="MLVSYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQVNVGEEEERQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVIIGTDK
                     APYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDN
                     FEGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKS
                     SRVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VRVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKYDGTAGELTATQKLRRQVRTIAE
                     GVGLTEIITYTLTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPREELPNEINSFAFALTGLVAEKDFQTVAVPVDFFYA
                     KGILEALFARLGLQVTYTATSEIASLHPGRTAAISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSAIEAALQPAAPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNA
                     EVR"
     gene            complement(11978..12979)
                     /locus_tag="VW854_001167"
     CDS             complement(11978..12979)
                     /locus_tag="VW854_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV0262405.1"
                     /translation="MIKTFLSALSVILFVIPIIAYSFFPSSNLNNWLSTRPILAQIYA
                     FPLATATMASILSFVFFSLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NVLPPFERGTWNSQSPKLFNTTIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            13135..13538
                     /locus_tag="VW854_001168"
                     /pseudo
     CDS             13135..13538
                     /locus_tag="VW854_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            13664..13930
                     /locus_tag="VW854_001169"
                     /pseudo
     CDS             13664..13930
                     /locus_tag="VW854_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            14191..16440
                     /gene="metE"
                     /locus_tag="VW854_001170"
     CDS             14191..16440
                     /gene="metE"
                     /locus_tag="VW854_001170"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEV0262406.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVSAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLTVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEVILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            16504..17370
                     /gene="metF"
                     /locus_tag="VW854_001171"
     CDS             16504..17370
                     /gene="metF"
                     /locus_tag="VW854_001171"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEV0262407.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNDNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQTPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            18057..20270
                     /gene="pnp"
                     /locus_tag="VW854_001172"
     CDS             18057..20270
                     /gene="pnp"
                     /locus_tag="VW854_001172"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEV0262408.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDAILTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYANHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHQKDYKPKKEFTETSKDSE"
     gene            20286..20903
                     /gene="cysE"
                     /locus_tag="VW854_001173"
     CDS             20286..20903
                     /gene="cysE"
                     /locus_tag="VW854_001173"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEV0262409.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KHGFKLLARMYSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            20915..21799
                     /locus_tag="VW854_001174"
     CDS             20915..21799
                     /locus_tag="VW854_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0262410.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGELVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKAGQVIGTCTVDLSTNT
                     NYLYGLAILEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            21881..23224
                     /locus_tag="VW854_001175"
     CDS             21881..23224
                     /locus_tag="VW854_001175"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEV0262411.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            23217..23603
                     /locus_tag="VW854_001176"
     CDS             23217..23603
                     /locus_tag="VW854_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEV0262412.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            23607..24491
                     /locus_tag="VW854_001177"
     CDS             23607..24491
                     /locus_tag="VW854_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEV0262413.1"
                     /translation="MRAKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            24628..25083
                     /locus_tag="VW854_001178"
     CDS             24628..25083
                     /locus_tag="VW854_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262414.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGYNFSSVSSS
                     SQTAKYEGTVYNNNSSLYITIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNAK
                     DNAGNWTSASNKKEVTVKIVSAKPTYPDKILVKNPDNIKDTEKMPLLKN"
     gene            25080..25226
                     /locus_tag="VW854_001179"
     CDS             25080..25226
                     /locus_tag="VW854_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262415.1"
                     /translation="MKEANKNHPAGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVT
                     KYAR"
BASE COUNT         7835 a         4478 c         5599 g         7531 t
ORIGIN      
        1 ctttatcatc ataagttaat ttcataataa aaacacccca aaagttagat tttttctgtc
       61 taacttttgg ggtgtagttc atgtacacct gatatgatgc gttttttgaa ttttatagac
      121 tgctcgttta aactctattt acctcgtaca ttccggagcg agacggaata ttagccacat
      181 acaaaatgag tactattagg attttatttt cgtgtacaat ttcagctatt cttgttataa
      241 tcagcctata ggaatcaagg aggggactct tatggctgtt tttgtgtctt tggatggaat
      301 tgtggtagaa gtacttgatg tcttttcttc ttttaatggg gatagtgagt ttttcttgtg
      361 taaacgtctt aaagacaaga gtcagtttgt tatggaacgc tctcagttcg aggagatgtt
      421 ccaacttcaa agtagtcgct tgacgacgca agaaaaatta caattgttta cctccgtgtt
      481 tgctggacgt tatgatgttt atgctaagaa ttttatcaat gaacaaggga aaattcagta
      541 ttttccttcc tatgattatg gttggaagca gttgccacct gaaaaacgga gtttccagac
      601 attgacgaac tccgttttga aatctcattt tcgtggggag gcagctatcg gtatctttcc
      661 tatgcactta gatgatagct gttatttttt ggtactggat ttggatgaag gagattggaa
      721 agaagctggt ttaaccattc gaagaatagc aagggaacgc cagatggaag cccatttaga
      781 gatttctcgt tcgggttacg gactccatat ttggttcttc tttgaggaag cgattctgag
      841 tcgaaaggct cgcctgtttg gaaagaaact attagaattg gctatgcagg aaagtatgca
      901 actgtccttt gattcttttg atggcatgtt tccaaatcag gatgttcttc ctaaaggtgg
      961 atttgataat ttaatttccc ttccttttca aggagaagct tatcatcaag ggcgaacggt
     1021 ctttgtggat gaacattttc agccttatga agatcaatgg aggtatctac aagaaattca
     1081 gaggatttca actgctaaag tggcactgtt aatccaagag gagttaggca agcaagaatt
     1141 ggataaggag ttgaaggtcg ttttatccaa tatgatccaa cttgaaaaat cgtctgtgac
     1201 atccaaggca ctttttttct tgaaaaatat ggcttccttt tctaatcccg aattttatag
     1261 tagattgaaa ctagaatagt atacctctgc ttctaaaaca ttgttagaaa tcgatttgac
     1321 tttcctgatc gatttgtcct gttattattt cattttatta tatttaaagc aggctatgcg
     1381 acagccaacc tatcaaattc ctgagcgaat gtatttattt ggagaatccg attattattt
     1441 atggctgcca agaggcttgt tatatccatt gcaagataaa tttaagcagg tagttgtgga
     1501 ggataggaga aaagtacaaa ggtctattag agtagcattt aagggagagc ttactttgga
     1561 gcaagaatta gccctgtcag atatgaattc taaagaaaat ggtttacttc atgcgggaca
     1621 ggttttggaa agatcgtttt aggtgctgct cttatctctg aacgtaagac aaaaaccatt
     1681 atcctagttc ataatagaca actcttagac caatgactgg atcgtttaaa gtgttttttg
     1741 actttcgaag aggcagaagc tacccgttat acaccatcag gtcgtgaaag ggtaataggc
     1801 tatgtcgggc agtacggtgg ggctaagaaa tgactgagta aactggttga tgttgttatg
     1861 attcaatctc tatttaagtt ggaaaatagt caaagtcttt tggatgagta tgagatgatg
     1921 attgtggatg agtgtcatca tatctctgcc ttgatgtttg aaaaagttgt tgctcagttt
     1981 agagggaagt atctttacgg tttgacggct acgcctgagc gtaagaatgg tcatgaacct
     2041 attgtttttc agagaaattg aaaagaccaa agcaagtaat tttatacagc tcagtgattg
     2101 gattgctaca gactcagcga ggaatcagtt gattctcaag gatattctag cccaagtggc
     2161 agaaggacgg aatatcttgg ttttagttaa tcgaattcaa cagatagatg tctttgaaaa
     2221 gttattgaaa gagaaagagg ttgatgactg ttacattatt agcggaaaaa ccaaagtccg
     2281 agaaagagaa cgagtttact ggagacgtta gaacagttag ataaagggtt tgttttgttg
     2341 tctactggaa aatacattgg cgaaggtttt gacttacctc agttggacac gcttatcttg
     2401 gcagcaccct tttcttggaa aaataatttg attcagtact caggtcggat tcatagaaac
     2461 tacaaggata agtctttggt gcggattttc gattatgtgg atattcatgt tccttattta
     2521 gaaaagatgt ttcagaaacg acaagtagct tatcgaaaga tggattatcg tgccattgag
     2581 ggtgaggaga aacaattcgt ttatgttgat agtagatatg agaaggtgtt gagagaggac
     2641 ttagcagggg aaagacagga atgtctgctt attttacctt atgtgcacca gacaaaactg
     2701 atgaattttc taaaagaatt taggattagt caaattgaga tatgtatacc agagacggtt
     2761 gcaaataaag catggctaga ccagttgaag agccagaaaa ttaaagtgtc ttttactcaa
     2821 tcaaaaatag taacgcctat tcttttggtg aataagacta ttgtttggta tggtgcaatg
     2881 ccattattag ggaaggtaga tgagatgacc atattacgtt tggaatcagc tagtatagtt
     2941 tctgaactag tggcaggttt acgatagaga aaatttttaa aaatttctat gtatgatttt
     3001 catttcttta gtgagactgt tgccattatc acattcgaat cacacaaaat aaaaaaattt
     3061 ttataagtac ttgacaaata gattgaaata tcataaaata aaaacggtta cagagttatt
     3121 aattatttaa gcttcatgtc accattaaaa attgaaataa aaggatgtta tcactaatac
     3181 aagtgagcag gaacctattt aatcacatca gaagaagttt cttgatgttt tttaagtagg
     3241 ttccttttat tttaaaaggg aaattttatg atcataaaac gaatactaaa ccacaatgcc
     3301 gtaattgcgc aaagtaaaaa agatatcgat attcttcttt ttggaagggg aatagctttt
     3361 ggaagaaaaa ttggagataa agtaaatcca attgatattg agaaaagttt ttttctcaaa
     3421 aatagagata atatgacccg ttttacagag atgtttatta acgttccttt ggagttggtg
     3481 tacatcaccg aaaaaataat taatctaggt aaaataacat taggtaataa ttttgatgag
     3541 attatctata taaatctaac ggatcatatt tcttcgagta tagaacgtta taaagaagga
     3601 gttattattt cgaatcccct acgctgggaa atatcgaaat attataaaga agaatttgag
     3661 cttgggaaaa gggctttaca aataataaaa aaagagttag gtattgaact tccaattgac
     3721 gaagctgcat tcatagcgct acattttgtt aatgctaatt tagaaaataa ttttcaagag
     3781 tcgtataaaa tcactgaaat aattatggga attgagaaaa tcattcaaga tttctattgt
     3841 actgagttta accaagattc tattgattat tatagattca taactcatat aaaattattt
     3901 gcccatcgct tggttgaaaa tacaacttat tgtgacgatg atgatgaaga cttgttagca
     3961 ttaatgaaaa ataaatatcc tagagaatat gaatgtggtg aacaggtggc aatgtttata
     4021 caaactgagt acaattattt gctgacttct agtgaattag tttatttgat ggctcatatt
     4081 cgtcgcttaa caaaaaattt agattaatag aataggagaa taccatgagt tataaagata
     4141 aggtacaaaa aatcctcgat gtaattggag gtgaaaaaaa tgtcaataga gttacccatt
     4201 gtgtaacacg tttaagatta gaattaaaag atgaaaattt agtcaatgat gatgatgtga
     4261 agaagatacc aggtgtaata ggtattatga aaaagaatgg acaatatcaa attatacttg
     4321 gtaatgatgt agctaattat tataaagaat tcgttaaact tggcaatttt gaatccgatt
     4381 cagttgttca agggcacaaa gggaatattt tagaaagaat cattgagtat atcgctggtt
     4441 ccatgactcc aatcattcca gcaatgttag ggggaggtat gctgaaagtc ttggtaatta
     4501 ttttaccaat gcttggtata ttgcaatcag attctcagac tattgctttt ttgacatttt
     4561 ttggggatgc tccatattat ttcttaccgc tgttattagc ttattctgca tcacaaaaat
     4621 taaaagtaac atctacatta gctatgtctg tagcaggtgt acttctccat ccaaattttg
     4681 ttcaaatggt gcaatcaggg aatcctctta gtttatttgg tgtacctgtg ataccagcta
     4741 gttatggttc gtcagtcgtt ccaattctta ttatggtttg gttgatgaaa tatattgaaa
     4801 aaatgattgc taaattaaca ccagctgtta ctaagagttt tttgcaacct acgctagtat
     4861 tattagtatc aagctgtatt gccttagttg tagtcgggcc tattggagta attgttggtg
     4921 aaggattatc aaatctagtt gggcaaatgt atgatgtagc tggatggctt acattagcta
     4981 ttcttggtgc tattatgcca tttattgtta tgactggaat gcattgggct tttgcaccta
     5041 tttttttggc ggcatctatt gctactccag acgtattaat tcttccagca atgttagggt
     5101 caaacttagc tcaaggggct gcttcgatgg ctgttgcatt aaagagtaaa aataataata
     5161 caaaacaaat tgcttttgca gcaggtttct cagccttact tgcagggatt accgaacctg
     5221 cattatatgg tgtgacttta aaatataaaa aaccgcttta tgcagctatg attggtggtg
     5281 gattagcggg attatttgca ggtcttacta gtgttaaagc gtatctattt gctgtcccat
     5341 ctttgatagc gttgcctcaa tttatttatt ctgatgtacc atcaaatatt gtaaatgctt
     5401 taattgtggc ggttatttcg gttgttatta cctttgtatt agcttatata tttggaatcg
     5461 atgaagaaga gagttctagc aatttagaag ttaaagctgg agtttcaaat aaaaaaatga
     5521 tattttctcc tatatcagga gaaatcattc cgttaagcga tgtccaggat aaaacatttt
     5581 cagataaact aattggagac ggagtagcga ttattccaag tgaaggtaag gtttatgcac
     5641 catttgatgg gaaaattaca aatatttttc cgactaagca cgcaattgga ttgaagagtg
     5701 atgagggtgt tgagttacta attcatattg gattagatac tgttgagcta aaaggtcaag
     5761 gttttattag tcatgtagaa gaaggagaca gagttttcaa aaatcagttg atttttgaaa
     5821 tggacttgaa tttaatcaag actaaaggct acgaaacagt tacaccagta attgtaacga
     5881 ataccaatga ttttctagat gtattagtat tacctaataa tcagacaatc gagcattcta
     5941 aggaattact ggtaatatta taatggagga aagttatgac catttttcca gatgattttc
     6001 tttggggtgg agctgttgca gctaatcaag tagagggagc atataatgaa gatggtaagg
     6061 gcttatcagt tcaggatgtg ttacccaaag gtggattagg agaagcaaca gaaaatccta
     6121 cagaagataa cttaaaattg ataggtattg atttttatca taaatataag gaagatatat
     6181 ccttgttttc tgaaatgggc tttaatgttt ttcgtacttc tattgcatgg agtagaattt
     6241 ttccaaaagg agatgaggaa gaacctaacg aagctgggtt gaaatattat gatgaattgt
     6301 ttgatgaact acatgctcac gggatagaac cacttgtaac tctttcacac tatgagactc
     6361 cattatattt agcaagaaaa tatcatggat ggattgatag gagaatgatt catttttatg
     6421 aaaaatttgc tcgaacagtt ttagaaaggt ataaagataa agttaaatat tggcttacat
     6481 ttaacgaagt aaactctgtt ttggaattac cgtttactag tggaggaata gatataccta
     6541 aggagaatct ttcgaaacaa gaattatatc aagctataca tcatgaatta gtcgcctcga
     6601 gtttggttac aaaaattgct cgtgagatta attcagagtt taaggtgggg tgtatggtat
     6661 tagctatgcc agcttatcca atgactccaa atccaaaaga tgtatgggcg actcatgagt
     6721 atgagaatct aaattatcta ttttcagatg tgcatgttag aggttattat ccgaattatg
     6781 caaaaagata ttttaaggaa aatgacatta acatagagtt tgcagctgaa gatgcagagt
     6841 tattaaaaaa ttatactgta gattttttat cctttagcta ttacatgagt gtgactcaat
     6901 ctgctcttcc aacacagtat aattcaggag aagggaatat tattggtggt ttagtaaatc
     6961 cttatttaga gtcttctgag tggggatggc aaattgatcc aattggacta cgtataattt
     7021 taaatagata ttatgaccgt tatcaaattc cattatttat agtggaaaac ggattaggtg
     7081 ctaaagatca actaataaaa gatgaattta ataacttaac agtccaagat gattatagaa
     7141 ttcagtatat gaaagaacat ttattgcaag tagctgaagc tctacaagat ggtgtggaaa
     7201 ttatgggcta tacgtcttgg ggatgtattg actgcgtttc tatgtccact gcacagcttt
     7261 ctaagaggta cggtctcatt tatgttgatc gaaatgatga tggaaatggc acattcaatc
     7321 gatataagaa aatgtccttt acttggtata aaggagtgat tgaatcaaat ggagaatcct
     7381 tattcaaata gttttttatt gatgactagc tattatcacg gttaaggtgg catttcatta
     7441 aatatggaat aaagaagttt ggaagacatt aaattctaaa cttctttatt ttatagggga
     7501 gtgtcaatta atttaatttt gttacaaaaa ttataaatag ttaatttaga taaattgttt
     7561 atataaattt agataagaat aagctaacaa gcagaagtaa gataggtctt gcaagaatgg
     7621 taaaaatctg ataaactaag tagtaattga ataggaatct gaaagactag tagcccaagg
     7681 gaagtatatc agggaggaga gccgtgactg caagctctct atatgaaagc tgggtgaatt
     7741 cacttgcgca tggatttaga aatgaaggtc tgacttgtca gatgaaaacg gatggtaccg
     7801 cgtgtcaacg ctccgagtgg agtttttggc atgtggtttt ctttttatct acgagagact
     7861 gatggaggaa atatgtcaac tattgaagaa caattaaaag cgcttcgcga agaaacgctg
     7921 actagcttga agcagattac tgctggaaat gaaaaagaga tgcaagattt gcgtgtctct
     7981 gtccttggta aaaagggttc gctcactgaa atcctcaaag ggatgaaaga tgtttctgct
     8041 gagatgcgtc caatcatcgg gaaacacgtc aatgaagctc gtgatgtctt gacagctgct
     8101 tttgaagaaa cagctaagct cttggaagaa aagaaagtcg cggctcaact ggctagcgag
     8161 agtatcgatg tgacgcttcc aggtcgtcca gttgcgactg gtcaccgtca cgttttgaca
     8221 caaaccagtg aagaaatcga agatatcttc atcggtatgg gttatcaagt cgtggatggt
     8281 tttgaagtgg agcaagacta ctataacttt gaacgtatga accttccaaa agaccaccca
     8341 gctcgtgata tgcaggatac tttctatatc actgaagaaa tcttgctccg tacccacacg
     8401 tctccagttc aggcgcgtgc tatggatgcc catgattttt ctaaaggtcc tttgaagatg
     8461 atctcgccag ggcgtgtctt ccgtcgcgat acggacgatg cgacccacag tcaccaattc
     8521 caccaaatcg aaggcttggt agttgggaaa aatatctcta tggctgatct tcaaggaacg
     8581 cttcagttga ttgtccaaaa aatgtttggt gaagagcgtc aaattcgttt gcgtccatct
     8641 tactttccat tcacagagcc atctgttgag gtggatgttt cttgcttcaa atgtggtgga
     8701 gaaggctgta acgtatgtaa gaaaacaggt tggatcgaaa ttatgggggc cggtatggtt
     8761 cacccacgtg tccttgaaat gagtggtatc gatgcgactg tatactctgg ctttgccttt
     8821 ggtcttggac aagagcgtgt agctatgctc cgttatggaa tcaacgatat ccgtggattc
     8881 taccaaggag atgtccgctt ctcagaacag tttaaataat gattagaaaa gtagaagtgg
     8941 cagatgttga ggtgttggct aaaattgcca aacaaacctt tcgtgaaacc tttgcgtatg
     9001 ataatacgga agagcagtta caggaatact ttgaagaggc ttatagtcta aaaactttgt
     9061 caactgagtt gggaaatcct gactctgaaa cctatttcat tatgcatgag gaggagatag
     9121 ctggttttct caaagtcaac tggggaagtg ctcaaactga gagagaatta gaggacgctt
     9181 ttgaaattca acgcctctat gtgctacaaa aattccaagg atttggacta ggtaagcaac
     9241 tgtttgaatt cgcacttgaa cttgctacaa aaaatagttt ttcttgggct tggttaggtg
     9301 tttgggagca taatacaaaa gctcaagcgt tttataatcg atatggtttt gaaaaattta
     9361 gccaacatca ttttatggtt ggtcaaaaag tagatacgga ttggttactg agaaagaaat
     9421 taaggtaaga agaggattct tctattgaaa tgataggaaa ggaaaagaac tagagtgaca
     9481 actgtcattc tggagaacta aattatgctt gtatcttata aatggttaaa agaattggtg
     9541 gacattgatg tgccatcaca agagttggct gaaaaaatgt caactacagg aatcgaggta
     9601 gagggtgtcg aatcaccagc tgctggtctc tcaaaaattg tcgtcggtga ggtcttgtct
     9661 tgcgaagatg tgccagagac tcacctccat gtttgtcagg ttaacgttgg cgaagaagaa
     9721 gagcgtcaga tcgtttgtgg tgccccaaat gtgcgtgctg ggatcaaggt catggtggct
     9781 cttccaggag ctcgtatcgc tgataactac aaaatcaaaa aaggaaaaat ccgtggtttg
     9841 gagtcacttg gaatgatctg ttcacttggt gaattgggga tttctgactc agttgtgcct
     9901 aaggaattcg cagatggcat ccaaatcttg cctgaagatg ccgtgccagg tgaggaagtc
     9961 ttttcttacc tagacttgga tgatgaaatc atcgaacttt ccatcacacc aaaccgtgca
    10021 gatgcccttt ctatgtgtgg agtggctcac gaagtggcag ccatctatga caaggcagtc
    10081 aactttaaag aatttactct aacagaaact aatgaagctg cggcagatgc cctttctgtc
    10141 atcattggga cagacaaggc gccttactat gcagctcgta tcttggacaa tgtgactatc
    10201 gcaccaagtc cacaatggtt gcaaaacctt ctcatgaacg aaggaatccg tcccatcaat
    10261 aacgtagtgg acgtgaccaa ctacatcctg ctctattttg gtcaaccaat gcatgccttt
    10321 gacttggata actttgaagg gactgacatc cgtgtgcgtg aagcgcgtgc tggtgaaaaa
    10381 ttggtgacct tggacggtga agaacgtgac ttggacgtga atgacctagt catcactgtc
    10441 gcagacaagc cagtagccct tgcaggtgtc atgggtggtc aagcaacaga aatctctgaa
    10501 aaatctagtc gtgttgtcct tgaagctgct gttttcaatg gcaaatctat ccgtaagaca
    10561 agtggtcgcc tgaaccttcg ttctgagtca tcttctcgct ttgaaaaagg aattaatgtg
    10621 gcaacagtta atgaagccct tgatgcggca gctagcctga ttgcggaact tgcaggtgcg
    10681 acggtgcgta agggcatcgt ttcagcgggt gagcttgata cttcagatgt agaagtttct
    10741 tcaacccttg ctgatgttaa ccgtgtcctc ggaactgagc tgtcttatgc tgatgtagaa
    10801 gacgtcttcc gtcgtcttgg ctttggtctt tctggaaatg cagacagctt tacagtcaga
    10861 gtcccacgtc gtcgttggga tatcacaatc gaagctgacc tctttgaaga aattgctcgt
    10921 atctatggtt atgaccgctt gccaactagt ctaccaaaat acgatggtac agcaggtgaa
    10981 ttgacagcca cacaaaaact ccgccgtcaa gttcgtacta ttgctgaagg agtaggtttg
    11041 acagaaatca tcacctatac tctaacaact cctgaaaaag cagttgagtt tacggctcaa
    11101 ccaagtaacc ttactgaact tatgtggcca atgactgtgg atcgttcagt cctccgtcaa
    11161 aatatgattt caggtatcct tgataccgtt gcctacaacg tggctcgtaa gaataaaaac
    11221 ttggcccttt acgagattgg aaaagtcttt gaacaaacag gtaatccaag agaagaactt
    11281 ccaaatgaaa tcaacagttt tgcctttgcc ttgacaggct tggttgctga aaaagatttc
    11341 caaacagtag cagttccagt tgatttcttc tatgctaagg gaatccttga agcgctattt
    11401 gctcgtttgg gactccaagt aacctataca gcaacatctg aaatcgctag ccttcatcca
    11461 ggtcgtacag ccgcgatttc actcggtgac caagttcttg gtttccttgg ccaagtgcat
    11521 ccagtcactg ctaaggctta tgatattcca gaaacgtatg tagctgagct taacctttca
    11581 gctatcgaag ctgcgcttca gccagcggct ccatttgtgg aaatcacgaa attcccagca
    11641 gtcagccgtg acgttgccct tctcctcaag gcagaagtga ctcaccaaga agttgtagat
    11701 gctatccaag ctgccggcgt gaaacgtttg acagatatca aactctttga cgtcttctca
    11761 ggtgaaaaac tgggacttgg tatgaagtca atggcttata gcttgacctt ccaaaatcca
    11821 gaagacagct taacggacga agaagtcgca cgctacatgg aaaaaatcca agcgtctctc
    11881 gaagaaaaag tcaatgcaga agtgcgttaa acgatagtag gatacaaatt ccccctctag
    11941 tttatgaaca ctagaggggt gattttatat aaaataatta aaatgtgatt tctgtaaaaa
    12001 tacatctatg atcagagttt tgaaaactta caatgtctaa atctttaaca tagtagtggt
    12061 ttttaggcaa taaaatatga tctattgttg tattaaaaag ttttggactt tggctattcc
    12121 aagttcctct ttcaaaaggt ggcagtacat ttaatgcgtc cctatgagag cttatttttg
    12181 caagtgctcc atgacgcata gttgcattaa aatcacctgc aataatagcc tttggatatt
    12241 ttgaagccaa ttgattatga atgatgttta agtcttgctt ccagatttcc attaaacctg
    12301 gcagaggagg agcagtatgc aaggcaatga tatctggtat attttgtttt ctcgaatgta
    12361 atacaattgt cccgaaccgt gttgtatgaa aagttttagc ttctgtatag aaaccataac
    12421 ttttcttgac aatcacagtc acaggagcta ttccactatt ggtaggtgga gaagtgaaaa
    12481 tatcatagtt ggccatagaa agtccaactt gatgaaacaa tagtttgatt ctctggtttt
    12541 cttgctcacc tctgatattg gtagctagtt cagggaatat agccatatcg gcgtcaaaat
    12601 ggctaaaaat tcgctcaata tgttgtgctt ctatttgatt agcgacgttc caagttacta
    12661 attttaaggt tttagtctta tttgatgcag aagaaagggt tttatctgtt ccgaataata
    12721 gtaatatgag cgatagtaag agcaaaatgc cagagtaaaa ccgtatttgt ttatttttct
    12781 tgtaaaaaga tagggaaaaa aatacgaaac ttaaaataga agccatagtt gcagtagcta
    12841 aggggaaggc ataaatctgt gccaagatag gtctggtaga tagccaatta ttaagattag
    12901 aagatggaaa aaaagaataa gctatgatag ggataacaaa gagaatgacc gaaagggcag
    12961 agagaaatgt tttaatcatt gtgtgagtac ctcaatttgc aatggttgct cggagctaat
    13021 gtgactttgt gactttactt gattgatatt ctttttattg tcttccttat agattaagta
    13081 caacccaagt aaaaaaagtc caatttaaga ttgaaatgat taaaaggcac agaaatgcaa
    13141 gacaattata ctacaaaagg caaacatttg acaatcgata gccgtcgctt aatcgaacga
    13201 tggaaaaaag aaggaaaatc aaatagagaa attgcctctc tacttggaaa agctcctcaa
    13261 actatccaca ctgaaataaa gcgtgggaca gtccgacaat gtcttggaaa agggcgcttc
    13321 aaagaggttt attctgccga ctacgctcaa cagtcttaag aaactaaagg aaaagattct
    13381 ccactataca taaccaaaaa ttttcgcctg aaatgatggt tatggttaaa ggggttaacg
    13441 tgggaatttc aaccatttac tattggattc atcgtggaaa attggggtta agcaagcagg
    13501 atctgcttta tcctagaaaa ggaaaatcgc ttaagaaata ggctagcacc aactttaaac
    13561 ccactggtca atctatcgaa tcataattcg ctcatttttt taaaaataca tagacgtaaa
    13621 aaaaagaggg ttaagtgatg aagaatagtg ctattgggag taattggaag gatgtccatc
    13681 tgagctcttt agcaaggagg aaatccttga aagtgatatg cgagtggcta tcatgagcga
    13741 gttgattgag gctaggtatg aacaaggaat cagtcagaaa aagctggaag aagtcagtgg
    13801 aataagccag cctgtcatag ctaggatgga gacaggaaag actagtcctt agttggatac
    13861 agtcttaaaa gtcctagcca gtttaggaaa gacactagca gtcgtcccac ttgaacaggg
    13921 aaaaagttga ctagaatcag ttatagtctt ttctaataac aggtcatagt cacttataag
    13981 aattactaat aacatgtttt agtattctaa ttggaatata gctgtaaagt aagaattaca
    14041 aaggatttga gacgtttgct tcaagtcttt ttcttttgtc cgaaacagat atatagtttc
    14101 aaactatatc aaaccctaat tttttacaat tatacagacg ctttcttttc tgttaagata
    14161 gtttcaacaa caaattttgg aggacacatc atgtcaacta caatcatcgg tttccctcgt
    14221 ttgggcgaat tccgtgaatt aaaatttaca actgaaaaat actttagaaa agaaatctca
    14281 gaagaagaac ttttagcagc agcaaaagac ttgcgtgcta aacactggaa cattgtcaaa
    14341 gaaaaaggca tcactgaaat tccatcaaat gacttttctc actatgataa cttcctagat
    14401 gcggctttcc ttttcaacgt ggtgcctgca tccgttcaaa acttggactt gtctgacctt
    14461 gagcgctact tcgctttggg acgtggttac cagggagaaa aaggggacgt tcgcgccctt
    14521 ccaatgaaga aatggttcaa cactaactac cactacatcg ttcctaaatt tgaaaaagac
    14581 actcaagtaa aattggctgg tcacaagatt ttcgatgagt tccaagaagc caaagaactt
    14641 ggattgaaca ctcgtcctgt tcttgtaggt cccttcactt tccttcaatt gtcagacttt
    14701 gaagaaggcg tgaaagcaga cgacttcgta gatagcttag tgtctgctta ccaagaagtt
    14761 tttgctaaat tggctgaact tggtgcgact cgcatccaat tggatgaagc ggctcttgta
    14821 aaagacttga cagctgaaga aaaagctctt ttcttgaacc tttacaacaa actcttggct
    14881 gacaaaaaag gtcttgaagt cttgcttcaa acttatttcg gtgatgttcg tgacgtatac
    14941 gctgaccttg taaacttacc agtagatgct atcggtcttg actttgttga aggtaagaaa
    15001 actcttgaac tcgttaaagg tggcttccca gctgacaaga ctctctatgt aggtattgtc
    15061 aatggtaaaa acatctggcg caacaactac gaaaagagct tgactgttct tgagcaaatc
    15121 ccagctgaaa acatcgtttt gacaagctct tgctcacttc ttcatgtgcc atttacaact
    15181 gctaatgaag aatttgaacc agcactcttg aaccactttg cctttgcagt tgaaaaattg
    15241 gatgaaatcc gtgacttgga tgctatccga aatggtcaag gttcagaagc tcttgcagcc
    15301 aacaaagaac tctttgcgac tgagcgtgtt ggtgaaaatg ctgaacttcg tgcgcgtatc
    15361 gctggcttga cagacgcaga ctacactcgt ttgccagcct ttgcagaacg tgaagctatc
    15421 caagaagaag ctttcaaact tccagctctt ccaacaacaa cgattggttc attccctcaa
    15481 acaaaagaag ttcgtgccaa acgtttggct taccgtaaag gtgaattgtc tcaaaaagag
    15541 tacgacgctt tccttgctga aacgattgac gaatggatca agtggcaaga agatattgac
    15601 tttgatgtcc ttgttcacgg tgaatttgag cgtaatgaca tggttgagta cttcggtcaa
    15661 aacttgtcag gatacctctt ctctaaaaat ggatgggtac aatcatacgg tatgcgtggg
    15721 gtaaaaccac caatcatctg gggtgatgtc actcgtctta accctatcac tgttaaatgg
    15781 tctagctatg cacaaagtcg tacaaacaaa cctgttaaag gtatgttgac tggacctgtt
    15841 accatcctta actggtcatt cccacgtgaa gacatctcta tcaaggattc aactctccaa
    15901 atcgcccttg ctatcaagga tgaagtactt gaccttgaag ctgctggtgt gaaaatcatc
    15961 caaatcgacg aggctgctct tcgtgaaaaa ttgccactcc gccgtagcga ctggtacgaa
    16021 gactaccttg actgggctat tcctgccttc cgcttggtac actcaacagt agcgccagac
    16081 acacaaatcc acactcacat gtgttactca gaatttacag atatcatccc agctatcgat
    16141 aacatggatg cagacgttat ttcctttgaa gctagccgtt caaaccttga aatcttggac
    16201 gaactcaaag cgaaaaactt ccaaacagaa gtgggacctg gggtttacga tatccactca
    16261 cctcgtgtac caaatgaagg cgaaatcgac aacacaatcg aagtcatcct tgctaaagtg
    16321 ccaagcaaga aagtttggat caaccctgac tgtggtttga aaacacgtgg tatcccagaa
    16381 acaaaagaaa gcttgatccg ccttgttgaa gcagctaaag ctgcgcgtga gaaattgtaa
    16441 gattggattt ctctccatac agtggttgtt caaaaagaaa tcaactagaa aaggttatta
    16501 catatgtcac gccaaacacc gtcactctca tttgaagtgt tccctccaaa cccagccgtg
    16561 ggtaatgata acattatttc agccttgcag gatatgcagg agttggctcc ccattttatc
    16621 agtgtaactg ccagcaataa taaatttaat atcaaggaaa cgacggttcg tttggctgac
    16681 tttatccaaa atgatttggc gattccgacc attgcccact tgccagctat ttatctaact
    16741 aaggacaagg ttgctgaaac tattgctgac ttggacaaag ttggggtgca gaaaatcttg
    16801 gctcttcgtg gggatattat tccagacgtg gaaccacaaa aggatttccg ctatgcaaca
    16861 gacttgattg agtttatcaa ggaacaaacc cctcactttg atattattgg agcttgctat
    16921 ccagaagggc atccagattc gccaaatcag atttcagata ttcaaaatct taagaagaaa
    16981 gtggatgcag gctgttcgag cctcgtaact cagctcttct ttgacaatga gcgcttctat
    17041 gactttcagg acaagtgcat cttggctggg attgatgttc ccattcatgc aggaattatg
    17101 ccaattctga atcgaaatca ggctctccga ctcttgaaga cttgtgagaa tatccatctt
    17161 ccacgcaaat ttaaagccat cttagacaag tatgagcatg accctgagtc gctcagagca
    17221 gcaggacttg cctatgcagt ggaccaaatc gtggacttgg taactcagga tgttgccggt
    17281 gtgcatctct atacgatgaa taatgctgat acagcaaaat acatccatca agcaacccat
    17341 gccttgttta atcaccagtc tctaggataa taaaagcaaa ccattcttct caggtgaggg
    17401 gaatggttcc tttttaatgg taaagacctc actttttaag aaaaatatga taaaataaat
    17461 tctgtacgta cttgatacaa agatgaaggt attaaataat acaatgcatt taaactgtaa
    17521 gatttaacga cacgagtgag tcaaatcgat gttatttgac gagagagtta gtaaagcatt
    17581 taactaatcg cccgatagcg attagcgtga aagctttaga tactttagta ttaaaccttt
    17641 ctaatgattt atatcttgaa aactgaactc gcctacaact ctgtggaaaa agataaatct
    17701 ccctaggagc agtcgctccg tcgttcgatt tcctattttc ctttgagttg cttgacggct
    17761 tagtatcttg atgacttgaa ttttggtctc atttcttagg aaaaaagata agcttcctag
    17821 cactcgtcga gtgcgacgtc accttcctat ttttctacag aaatgttcgg caagccgaac
    17881 cgtccaaaat atcttgtgta attgaacacg gccgaaaagc tgtgtaaaaa agataaactg
    17941 tcttgtcttc atcgaagact tcgtcagttt cctattttta ctttgctttt gacgtccttg
    18001 gtatcttgat ctttgtaggc aaggcgtata atttcatcaa tccaaagggg attaaaatgg
    18061 caaaacaagt gtttcaaacg acttttgcgg gtcgtgagtt aattgtagaa actggtcagg
    18121 ttgctaagca agcaaatggc tctgttgtcg tacgttacgg tgagtcaact gtcttgactg
    18181 ctgccgttat gtctaagaaa atggcaactg gggatttctt cccactccaa gtcaactacg
    18241 aagaaaaaat gtatgcggct gggaagtttc ctggtggctt tatgaaacgt gaaggacgtc
    18301 cttcaacaga tgcgatcttg acagcgcgtt tgattgaccg tccgattcgt cctatgtttg
    18361 cggaaggttt ccgtaatgaa gtccaagtca tcaatacagt gctttcttat gatgaaaatg
    18421 catctgcacc aatggctgct atgtttggtt catctttggc actgtctatt tcagatattc
    18481 catttgacgg accaattgct ggggtacaag tgggatatgt agatggccaa atcatcatca
    18541 acccaagtca agaacaagca gagcaatctc ttcttgaatt gacagtagct ggaaccaagc
    18601 acgctatcaa catggtagag tctggtgcca aagaattgtc agaagaaatc atgttggaag
    18661 cgcttcttaa agggcacgaa gctgtcaaag aattgattgc cttccaagaa gaaatcgttg
    18721 ctgctgtcgg taaagaaaaa gcagaagtgg aattgcttca cgtggatgct gaattgcaag
    18781 ctgaaatcat tgcagcctac aacagtgacc tccaaaaggc agttcaagta gaagagaaat
    18841 tggcccgtga agctgcaact caagcagtga aagaccaagt gactgccgtt tacgaagaaa
    18901 aatatgcgaa ccacgaagaa tttgaccgta ttatgcgtga tgtggctgaa atcttggaac
    18961 aaatggaaca cgcagaagtg cgacgtttaa ttacagaaga caaggtgcgt cctgatggtc
    19021 gtaaggtcga tgaaatccgt cctttggatg cggttgttga cttccttcct cgtgtacatg
    19081 gttcaggtct ctttactcgt gggcaaactc aagctctttc agtcttgacc ttggctccga
    19141 tgggagaaac tcaaatcatt gatggtttgg atccagagta caagaaacgc tttatgcacc
    19201 actataactt ccctcaatat tctgtagggg aaacaggtcg ttacggtgcg ccaggtcgtc
    19261 gtgaaatcgg tcacggtgcc cttggtgagc gtgctcttgc tcaagtcttg ccaagcttgg
    19321 aagaattccc atacgctatc cgtctagtag cagaagtttt ggaatcaaac ggttcttcat
    19381 ctcaagcttc tatctgtgcg ggaactcttg cccttatggc tggtggtgtg ccaatcaagg
    19441 cgccagtagc tggtattgct atgggactta tctcagatgg aaataactac acagtattga
    19501 cagatatcca aggtttggaa gatcactttg gagatatgga cttcaaggtt gcaggtactc
    19561 gtgatgggat tacagccctt caaatggata tcaagattca agggattact gcagaaatct
    19621 tgacggaggc tcttgctcaa gccaagaaag cgcgttttga aatccttgat gtcattgaag
    19681 caaccattcc agaagttcgt ccagaattgg ctccaactgc tccgaaaatt gatacgatca
    19741 agattgatgt ggacaagatt aagattgtca tcggtaaggg tggagaaacc atcgacaaga
    19801 ttatcgctga aacaggtgtt aagattgata tagacgaaga aggaaatgtg tctatctact
    19861 ctagtgacca agatgctatt aaccgtgcca aagaaattat tgctggtttg gttcgtgaag
    19921 ccaaagtgga tgaagtttac cgtgctaaag tcgttcgtat cgagaaattt ggtgcctttg
    19981 ttaacctctt tgataagaca gatgcccttg ttcatatctc tgagatggct tggactcgta
    20041 ccaatcgtgt agaggatttg gtagaaatcg gggatgaagt tgatgttaag gttatcaaaa
    20101 ttgatgaaaa aggccgtatc gatgcctcta tgaaggctct tctacctcgt ccgccaaaac
    20161 ctgagcatga tgaaaaaggt gaaaagtctg agcgccctca ccgcccacgt catcaaaagg
    20221 attacaaacc taagaaagaa tttacagaaa catcaaaaga ttcagaataa gaaaaggaga
    20281 aatgtatggg gtggtggcgc gaaaccattg atattgtaaa agaaaatgat ccagcggccc
    20341 gcaccacttt ggaggttttg ctgacttatc caggtgtcaa ggccttggcg gcccaccgtc
    20401 tctcgcattt tctctggaag cacggcttca aactattagc tcgtatgtac agtcagtttt
    20461 ggcgcttttg gactcagatt gagattcatc caggagccca gattgattca ggtgttttta
    20521 ttgaccatgg ttctggtctg gtgattggag agacagcgat tgttgaaaaa ggcgttcttc
    20581 tctatcacgg agtgactctc gggggaacag ggaaagactg tggcaaacgc catccgactg
    20641 tacgaaaggg agccctcata tcagcccatg cccaagttat cgggcctgtg gaaattggtg
    20701 aaaatgccaa ggtcggtgct gcagcagttg tcgtagcaga tgtacctagt gacgtgacgg
    20761 ttgtcggtat tccggccaag attgtccgtc ttcatggtaa gaaagatgag cctgtcattc
    20821 acgaagtcga agaaaaacga gagtattatg tcaataaact cgagcaggct aaagatgcca
    20881 gtcacagatc gtctggtttg tagaggatac ctatatgatt caagcaagaa acaagttaag
    20941 ccaagaggag ctatctgagg cgaaaaaagt aattaactgt tgccaaaact atgacggtac
    21001 ctatcgcgat ccctatctct ctaacatgct taattttgac ccaaacatgc ccgccttttt
    21061 cctttattat gaaaaaggcg aacttgttgg tttattaact gtctatgcag atgaccaaga
    21121 tgtagaagtg acgatactgg ttcatccagg tcatcgccgt caggggattg cgcgtgcatt
    21181 gtttactagt tttgagagag aaacagcttc tttccccatt cggtcagtca cttttcagac
    21241 agaacgtatt tttttagaga atcatcctga ctttgccagt aactggggac taatcgagga
    21301 tgaagagaca gaaacttggt taggtaagga tagaagacct tatccgttag caaatgtttc
    21361 ccatcttgaa gttttgttag cagatagttc gtatcaggat caaattagtc aattaaaatt
    21421 tcaggcattt tcagaggaac atgaatcgag agaagttgtg gatagatatg tcgctgaagc
    21481 tctgaaagat ccagaaagcc gactatatat tttattaaaa gctggtcagg ttattggaac
    21541 ttgtacggtt gatttatcga ctaatacgaa ttacctctac ggtttagcaa tattggaacc
    21601 tgaacgtgga aaaggctatg gaagctactt agcaaaatcc ctcgtcaacc aactaattga
    21661 gcaaaatgac aaggaatttc agattgcagt ggaagatagc aatgtaggtg ccaaacgttt
    21721 gtatgaaaaa attggctttg tcaaacagac tcaggtggtt tatctgaatg agaaaggagc
    21781 aagggattcc gaagtgtaga gatattcgga ctgaaattta cttgaacttt tagtgatgaa
    21841 actaattgtt cttggatttc agctttcctg attatgattt atgattaaaa tctatgacac
    21901 catgtctcgt gatttgcgag aatttgtccc gattgaggac ggcaagatca agatgtatgt
    21961 ttgtgggcca acggtgtaca actatatcca cgtaggaaac gcccgttcga cggtagcttt
    22021 tgatacgatt cgtcgctatt ttgagtaccg tgggtataag gttgcctata tttccaattt
    22081 tacagatgtg gatgataaga ttatcaaccg tgccagggaa gaaggtatca cgcctcagga
    22141 ggttgcggat aagtacatcg ctgcctttcg tgaggatgtg acggcattgg gcgtgaaacc
    22201 tgcgactcgc catccgcgtg tagtggagtt tatggcagac atcatccgtt ttgtggaaga
    22261 cttgatcgag aaaggctttg cctatgagag tcaaggggat gtctatttcc gtgtagaaaa
    22321 atcccacaac tatgctaaat tggctaataa aaccttggaa gatttggagc taggtgcttc
    22381 aggtcgtacc gatgaagaaa cggctcgtaa ggaaaatcct gtagactttg ctctatggaa
    22441 atcttccaaa ccaggtgaga tttcttggga tagtccttgg ggacctggac gtccgggttg
    22501 gcatattgag tgttcagtca tgtcgacaga gattttaggt gataccattg atatccacgg
    22561 tggtggagct gacctagagt ttccacacca caccaatgaa attgcccagt ctgaagctaa
    22621 aacaggcaag gcttttgcta actactggat gcacaatggc tttgtcaata tcgacaatgt
    22681 caaaatgtct aagtccttgg gtaacttcat tacggtacac gatgccctta aaactcttga
    22741 tgggcaagta cttcgtttct tctttgcgac ccagcactac cgtaagccta tcaacttcac
    22801 ggaaaaggca gttcgcgatg ccgagaccaa tctcaagtat ctcaagaata cttacgaaca
    22861 accatttaca ggaaatgtgg atgcccaaga gttacaaaac tttaaagata agtttgtagc
    22921 agctatggat gaagatttta atgctgccaa cggtatcaca gttgtctttg aaatggccaa
    22981 atggatcaac tcagggaact atgatgcaag tgtcaagcaa gctcttgcag atatgttaga
    23041 aatttttgga attgtctttg ttgaggaagt tttggatgca gagattgaag acttgattca
    23101 aaaacgccaa gaggcgcgtg ccaatcgtga ctttgcgaca gcagaccaaa tccgtgacca
    23161 attggttact caaggaatta agctccttga taccaaggat ggagtgaggt ggacacgtga
    23221 ttgatgtcaa tctcattaac gggattgcgc tagcctttga gggggatgcg gtgtattcta
    23281 tgtatattcg ccgtcacctc atcctcaaag gtatgaccaa acccaataaa ctccatcaag
    23341 aagcaactaa gtacgtgtca gccaaggctc aggctcgcct gattgctctc atgttggagg
    23401 agcaggtcct aacggaaaaa gaagaagaaa tctacaaacg tggccgcaat accaatagcc
    23461 acacaaaggc taaaaatgca gatgtcgtga cttatcgtat gtccacggga tttgaagcgg
    23521 ttatgggcta tctccatatg actgagaatc tggaacgtct tgagagtttg gtttcatggt
    23581 gcatccaaaa agtggagggc tagaacatga gggcaaaaga actacaagac tggtttcctg
    23641 aggctcggat ttcagaccaa ccagtagaga aagagggcta tctcacgctc cctttagctt
    23701 ctcagcagtg gattttgctg gaggaagctg ggctcagcga gcgtgaaaag cagttggttg
    23761 cccttttgac ccagcaggag caggctcgtt cgctaaaccc ttggtattcc tatctggttg
    23821 agggcaaggg acaggcaccg caagttttta aaaagattca gttggtttat tgccatcttt
    23881 cttattttca gcaggaaaat ctggcttctt ggctagatat gatgcggact ctttttccga
    23941 attgtcagac agtgctacag gtcggagctc aggattatgt tttcgtgctt caacaagaca
    24001 aatacacttc tgtaagagat attttaagtg atacgattga agcggttgag tatgactttg
    24061 gacttcgtct ttctatcatg ttgggtcagg tttggtctca gacgggacat caagccctat
    24121 cagacttaat caaagctgag cgggatttgt tcaagacatg gtggcgtcag ggtcaccaag
    24181 gtgttcatac tttttctcag ctctatcttt ggagtatggg agaaagactc gtggacttga
    24241 agccaatcaa ggaatgtcta caccagatga ttttggatca agatcagatt caggaaatca
    24301 ttctctctct ttgggaaaat agtgctgttc tcactaaaac agcccagcaa ctctatctgc
    24361 accgcaattc tctccaatac aagattgata aatgggaaga gttgacaggg cttcagttga
    24421 aagagttgac cgacctgacc ttgtgttatc aattgatttt aggttctttg tcaactatag
    24481 ttggtttgta aagaagttaa tatttggaga agaggattgc catcttctcc atttttatgt
    24541 gcagagttat agtggcttga tgctgggaaa gtacactgtg actgctaaaa cattcctaga
    24601 agctgctttg atttccctaa tctatttatg caaattttat gttattttac tataacagtt
    24661 gttgctaagc caaataatag tggggaagtt catttagacg taagcattga agataatcag
    24721 ggaggtagtg ggtataattt cagttctgtt tcaagtagct cacaaacagc taaatatgaa
    24781 ggaactgttt ataataacaa ttcatcatta tatataacga ttgataaaac gtctgatgca
    24841 acagctcttt tgaaattaaa gttgaataat gttgataatc aacctgctac tgaagttcct
    24901 agttcaggaa ttactgtaaa attaaatgct aaagataatg ctggaaactg gacaagtgct
    24961 tcgaataaaa aagaagtaac agtaaaaatt gtttctgcta aaccgacata tccagacaaa
    25021 atcttagtga aaaatcctga taatataaaa gatacagaaa aaatgccatt attgaaaaat
    25081 tgaaagaggc aaataaaaat catccagcag gagctccaac ctttgctaaa ggtgaaggag
    25141 agcatgcaaa tgatattgta gcaacttatt cagatggtac aacttattat gtaccgttaa
    25201 atgatgtgac aaaatatgcg aggtagtggc tgtaccactc acttattcac ctccccgtga
    25261 tttgtagtag tgataggctt tctcactatt attataaaac aaaataaaga tcacaacact
    25321 ttttcattct gtgttgtgcc ttgagtgaaa cgaaaggaat gaattataaa tatgaaaagt
    25381 atagtcacta gcatagatga gcgcttgcgt actcgcctac gagtgattat ctggaagcaa
    25441 tgg
//
LOCUS       DAVKWZ010000028        24771 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000028 DAVKWZ010000000
VERSION     DAVKWZ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24771
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.028"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(<1..191)
                     /gene="blpI"
                     /locus_tag="VW854_001180"
     CDS             complement(<1..191)
                     /gene="blpI"
                     /locus_tag="VW854_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpI"
                     /protein_id="HEV0262416.1"
                     /translation="MNTKMMEQFSVMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAAT
                     GPITGGAATLICVGSGIMSS"
     gene            473..1060
                     /locus_tag="VW854_001181"
     CDS             473..1060
                     /locus_tag="VW854_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEV0262417.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLIGHYYQYRWFLLSPRNLG"
     gene            987..2630
                     /locus_tag="VW854_001182"
     CDS             987..2630
                     /locus_tag="VW854_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEV0262418.1"
                     /translation="MVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVI
                     TYILQQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDA
                     NSIIDALASTILSLFLDVSILILVGGVLLAQNPNLFLLSLISIPIYMFIIFSFMKPFE
                     KMNHDVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYS
                     ILQTSLKQGTKLVLNILILWFGAQLIMSSKISIGQLITFNTLFSYFTTPMENIINLQT
                     KLQSAKVANNRLNEVYLVESEFQVQENPVHSHFLMDDIEFDDLSYKYGFGRDTLTDIN
                     LTIKQGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINYQDIKNIDKKVLRRHINY
                     LPQQAYIFNGSILENLTLGGNHMISQEDILRACELAEIRQDIERMPMGYQTQLSDGAG
                     LSGGQKQRIALARALLTKSPVLILDEATSGLDVLTEKKVIDNLMSLTDKTILFVAHRL
                     SIAERTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            2641..4002
                     /locus_tag="VW854_001183"
     CDS             2641..4002
                     /locus_tag="VW854_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEV0262419.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVFIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLSNSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LVASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTSPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            4059..4187
                     /gene="blpC"
                     /locus_tag="VW854_001184"
     CDS             4059..4187
                     /gene="blpC"
                     /locus_tag="VW854_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEV0262420.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGGLWEDLLYNINRYAHYIT"
     gene            complement(4258..5598)
                     /locus_tag="VW854_001185"
     CDS             complement(4258..5598)
                     /locus_tag="VW854_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV0262421.1"
                     /translation="MNIAWILLYTLVTNGLEIVIFFKVDGIDLTFERIFKAFLFKILL
                     AFVFVMIGYMVGNVYLSYFMEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDGYSFTGLCIIIFNFFISLAFLKWLDYDFTSLRKEILDKAFQK
                     SLTQINWIMGGYYLVMESLSFFEYEQSIQSKTVRHLILVFYLLFFMGVIKKLDTYLKE
                     KLYERLEQEQALRYRDMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EVYGSVLKDSSQKLQNNKYDLGRLVNIRDKALKSLLAGKFLKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTIVSILCDNAIEASVEASQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQNQVFRQVLTVIHTE"
     gene            complement(5612..6349)
                     /locus_tag="VW854_001186"
     CDS             complement(5612..6349)
                     /locus_tag="VW854_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0262422.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSPRAHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINKLH"
     gene            complement(6354..6692)
                     /locus_tag="VW854_001187"
     CDS             complement(6354..6692)
                     /locus_tag="VW854_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0262423.1"
                     /translation="MKYMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            6968..7297
                     /locus_tag="VW854_001188"
     CDS             6968..7297
                     /locus_tag="VW854_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEV0262424.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKTETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(7465..8514)
                     /locus_tag="VW854_001189"
     CDS             complement(7465..8514)
                     /locus_tag="VW854_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0262425.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYLISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(8511..9242)
                     /locus_tag="VW854_001190"
     CDS             complement(8511..9242)
                     /locus_tag="VW854_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0262426.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            9310..9720
                     /locus_tag="VW854_001191"
     CDS             9310..9720
                     /locus_tag="VW854_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV0262427.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMNATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            9730..10017
                     /locus_tag="VW854_001192"
     CDS             9730..10017
                     /locus_tag="VW854_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262428.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIIDQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            complement(10351..11487)
                     /gene="dnaJ"
                     /locus_tag="VW854_001193"
     CDS             complement(10351..11487)
                     /gene="dnaJ"
                     /locus_tag="VW854_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEV0262429.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGQGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(11889..12245)
                     /locus_tag="VW854_001194"
     CDS             complement(11889..12245)
                     /locus_tag="VW854_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N7)-methyltransferase"
                     /protein_id="HEV0262430.1"
                     /translation="MSVLDDEYLKNTRKVYNDFCNQADNYEAAKGFIDNIPVECLTRY
                     REIILAEHDSCIKNDEAVRNFVTSVLLSAFVSAMVSAMISLEIQTYKFVIPFIIGMIW
                     TVVVFLMINWNYIGKY"
     gene            complement(12247..14070)
                     /gene="dnaK"
                     /locus_tag="VW854_001195"
     CDS             complement(12247..14070)
                     /gene="dnaK"
                     /locus_tag="VW854_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEV0262431.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(14550..15083)
                     /gene="grpE"
                     /locus_tag="VW854_001196"
     CDS             complement(14550..15083)
                     /gene="grpE"
                     /locus_tag="VW854_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEV0262432.1"
                     /translation="MAQDIKNEEVEEVEEVQEEEVVETAEETTPEKSKLDLANERADE
                     FENKYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDV
                     KKGLGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKG
                     YKLHDRILRPAMVVVYN"
     gene            complement(15110..16144)
                     /gene="hrcA"
                     /locus_tag="VW854_001197"
     CDS             complement(15110..16144)
                     /gene="hrcA"
                     /locus_tag="VW854_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEV0262433.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFHRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVNLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRIADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(16308..16817)
                     /locus_tag="VW854_001198"
     CDS             complement(16308..16817)
                     /locus_tag="VW854_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEV0262434.1"
                     /translation="MNSRVEFRIFTIVDLDKEEEYLHEMHLKGWRYRTSRFGLFYFDQ
                     CQPDDVIYRIYDSRFLKKYKHELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTTTFLGGLVVCTSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            17424..19757
                     /locus_tag="VW854_001199"
     CDS             17424..19757
                     /locus_tag="VW854_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV0262435.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFRPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKIIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDANGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGDPVKVLE
                     TGAKTVSGSTPGFVSEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            19770..21233
                     /locus_tag="VW854_001200"
     CDS             19770..21233
                     /locus_tag="VW854_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV0262436.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSNM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLDPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            21233..22801
                     /locus_tag="VW854_001201"
     CDS             21233..22801
                     /locus_tag="VW854_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV0262437.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRFSTLVEIVRG
                     GSPRPIKDYLTSEVDGINWIKIGDTEKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLT
                     NSMSFGRPYILNVDGAIHDGWLAISNYENSLNKDYLFYILSSNVVYSQFLSLISGAVV
                     KNLNSDKVASILIPLPPLSEQQRIIEAIESALEKVDEYAESYNRLEQLDKEFPDKLKK
                     SILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNS
                     YYEEVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCNQWSGFSIDLAR
                     FIDPETVHSYQKERLLRDGDLMWNSTGLGTLGRLAIYHENKNPYGWAVADSHVTVIRV
                     LSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKELLTKTIKEYLIPLPPLPEQSRIV
                     DKIEQFFAHIDALI"
     gene            22858..23655
                     /gene="psrA"
                     /locus_tag="VW854_001202"
     CDS             22858..23655
                     /gene="psrA"
                     /locus_tag="VW854_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HEV0262438.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
     gene            23666..24274
                     /locus_tag="VW854_001203"
     CDS             23666..24274
                     /locus_tag="VW854_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV0262439.1"
                     /translation="MVKLTTCYFCDYGSYYGNKDETTSYPIYEIPEAWRYIKFASLVN
                     FRIGKTPPRSEATFWGTEIPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTL
                     LMSFKLSIGKVAILDIPATHNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAI
                     KGKTLNSTSISELLIPISNHEEMKRIISKVDLLFQKVSQLFE"
     gene            complement(24222..24767)
                     /locus_tag="VW854_001204"
     CDS             complement(24222..24767)
                     /locus_tag="VW854_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV0262440.1"
                     /translation="MNWVVIKIKDIFSMNTGLSYKKGDLSINNKGVRIIRGGNIKPLE
                     FSLLDNDYYIDTQFISSEQVYLKHNQLITPVSTSLEHIGKFARIDKDYDGVVAGGFIF
                     QLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQALYNIPKTTLSELLIPLAPFE
                     EQELITQKVEKLFEKVNQLWK"
BASE COUNT         7982 a         4857 c         4509 g         7423 t
ORIGIN      
        1 gaggacataa ttcctgaacc tacacaaata agtgttgctg ctcccccagt tatcggacca
       61 gtcgcagcag ctaatagtac tgctccaata caaccaccga ttgcagatcc taaattgcct
      121 cttcctccac taactatttc gagttcttca ttatccataa cagaaaattg ttccatcatt
      181 tttgtattca tgacaaatac tccttttttc tttttttatt tttgtcttgt tgtaactttg
      241 ataagtttag tatatcatcg ttttttaaaa tttttcatcc agatcttgaa ttgtcatcga
      301 aacgtcttga attagctttt ttattttaag ccacctctaa atgtttaaaa aaaataattt
      361 ctaatcactt ttttaccatt caggaagttt taatgactat tcaagatttc ataaaatatg
      421 aacttagttt tatgacataa tagacctatc cactatatga aaagaattgc caatgacttc
      481 ttataaacgt acatttgttc ctcaaataga tgcgagagac tgtggtgtcg ctgccttagc
      541 ctcgattgct aaattctatg gttcagattt ttctctagct cacttgagag aacttgcaaa
      601 gaccaataaa gaagggacga ctgctcttgg cattgtaaaa gccgctgatg aaatgggctt
      661 tgaaacaaga cctgttcaag cagataaaac gctctttgac atgagtgatg tcccctatcc
      721 atttatcgtt cacgttaaca aagaaggaaa actccaacat tactatgttg tctatcaaac
      781 aaagaaagac tatctgatta ttggtgatcc tgacccttct gtaaaaatca ctaaaatgtc
      841 aaaagaacgc tttttctatg aatggactgg agtagctatt tttctagcta ccaaacccag
      901 ctatcaaccc cataaagata aaaagaatgg tctactaagc aagcttcctt cctctgattt
      961 tcaaacaaaa atctctcatt gcttacattg ttctctcaag cttattggtc actattatca
     1021 atataggtgg ttcttactat ctccaaggaa tcttggatga gtacattcca aatcagatga
     1081 aatcaacttt aggaatcatc tcagttggtc tggttatcac ctatatcctc caacaagtca
     1141 tgagcttctc cagagattat ctcctaaccg ttctgagcca gagattaagc attgatgtga
     1201 ttttatccta tattcgccat atttttgaac ttcccatgtc tttctttgcg acacgtcgta
     1261 caggagaaat catttcacgg ttcacagatg ctaactctat tatagatgcc ttggcttcta
     1321 ccattctttc tctttttctg gatgtttcta ttctgattct tgtaggaggc gtcttactgg
     1381 cacaaaaccc taatctcttc cttctttctc ttatttctat tcctatatac atgttcatca
     1441 tcttttcttt tatgaaacct tttgaaaaaa tgaaccatga tgtcatgcaa agtaattcta
     1501 tggttagctc tgccattatc gaagatatca acgggattga aactataaag tcgctcacga
     1561 gtgaagaaaa tcgctatcaa aatatagaca gcgaatttgt agattatttg gaaaaatcct
     1621 ttaagctcag taaatattct attttacaaa cgagtttaaa gcagggaaca aaattagttc
     1681 tgaatatcct tatcctatgg tttggcgctc aattaatcat gtcgagtaaa atttctatcg
     1741 gtcagctgat tacctttaac acactttttt cttactttac aactcctatg gaaaatatta
     1801 tcaacctcca aaccaaactc caatctgcga aggtcgctaa taaccgtttg aacgaagtct
     1861 atctagtcga atctgaattt caagttcaag aaaaccctgt tcattcacat tttttgatgg
     1921 acgatattga atttgatgac ctttcttata agtatggttt tggacgagat accttaacag
     1981 atattaatct cacgattaaa caaggagata aggttagcct agttggagtt agtggttctg
     2041 gtaaaacaac tttagccaaa atgattgtca atttctttga accctacaaa gggcatattt
     2101 ccatcaatta tcaggatatt aaaaacattg ataaaaaagt cttgcgccgt catattaatt
     2161 acctaccaca acaagcctat atctttaatg gctctatttt ggaaaactta actttgggcg
     2221 gtaatcatat gattagccaa gaagatattc taagagcttg tgaattagct gaaattcgtc
     2281 aagacattga aagaatgcct atgggctatc aaactcagct ctctgatgga gctggtctat
     2341 caggaggaca aaagcaacga atagccctcg ctcgtgctct tttaactaaa tctcctgttt
     2401 taatactaga cgaagctact agcggtcttg atgtcttgac tgagaaaaag gttatagata
     2461 atcttatgtc tctaactgat aaaaccattc tctttgtagc ccatcgtctc agtatagccg
     2521 aacgaaccaa ccgtgtcatt gttcttgacc aggggaaaat cattgaagtt ggtagtcacc
     2581 aagagttaat gcaggcgcaa ggtttctacc atcatctatt caataaataa ggagaatgtc
     2641 atgaatccta atctttttag aagcgtcgag ttttatcaga gacgttacca taactatgcg
     2701 acagtgttca ttatacctct ttcattacta tttactttca tcttgatttt ctcccttgtt
     2761 gccacaaaag aaattactgt tacttcccaa ggagaaatcg cccctacaag tgtcattgcc
     2821 tccattcagt caaccagtga taatcctatc ctagctaatc atttagtggc aaatcaagta
     2881 gttgaaaaag gggacttact catcaaatac tctgaaacaa tggaagaaag tcagaaaact
     2941 gccttagcaa ctcaattaca aagacttgag aagcaaaaag aaggacttgg aattttgaaa
     3001 caaagcttag aaaaagcgac tgatcttttt tctggcgagg atgaatttgg ctaccataat
     3061 acctttatga attttactaa acaatcccat gatattgaac tgggtatcac aaagactaac
     3121 accgaagttt caaatcaagc taatctttcc aatagcagtt catcagctat tgaacaagaa
     3181 attacaaaag ttcaacaaca aattggagaa tatcaagagt tgagagatgc tatcataaat
     3241 aacagagcac gcttaccaac tggcaatccg caccagtcaa ttttgaatcg ttatcttgta
     3301 gcctcacaag gacaaacaca aggaactgca gaggagccat ttttatctca aattaatcaa
     3361 agtattgcag gtcttgaatc atctatcgca agcctcaaaa ttcagcaagc tggtatcgga
     3421 agtgtagcaa cttatgataa cagtttagca accaaaattg aagtactccg cactcagttt
     3481 ttacagacag cctcacagca acaactaact gtggagaatc aattaacaga attaaaagta
     3541 caactagatc aagccacaca gcgtttggaa aacaatacct taacctcccc aagtaaaggt
     3601 atcgttcatc tgaacagcga atttgaaggt aaaaatagaa ttccaactgg tacagaaatt
     3661 gctcaaatat tccctgtcat cacagataca agagaagtac taatcactta ctacgtatct
     3721 tctgactatc tacctctact agataaagga caaactgtaa gattaaaact ggagaagatt
     3781 ggaaatcacg gcaccaccat catcggccaa cttcagacaa ttgatcaaac tcctaccaga
     3841 acagagcaag gaaatctctt taaattaacc gctcttgcaa aactatctaa cgaggatagt
     3901 aaactcatcc aatatggctt acaaggtcgc gtcactagtg taactacaaa gaaaacatat
     3961 tttgattatt tcaaagataa aattttaacc cattctgatt aattttcaga taacactcta
     4021 taactattta ttatcttatc aaaaaggaga atcataacat ggataagaaa caaaacctaa
     4081 cttcatttca agaactaaca actaccgaac tcaaccaaat tacaggtgga ggattgtggg
     4141 aagatttatt atataacatt aatagatatg ctcattacat cacataagaa cttcatcatc
     4201 caatacaact ataaaaaaat aagaccgaga aacaagtact ctcggtctta tttttcatca
     4261 ttctgtatgt atcacagtaa gtacctgacg aaagacttga ttttgacagg tggtatttag
     4321 actggtatta ggatggcttt ccacaatctt catgacggta tagagaccaa ctcctctctc
     4381 ctccccttta gaactggctc caaaggagaa gatttcagaa atatcgatgc cctcttcttt
     4441 gatggagttt tcgatgataa aggtctcctg tgctccattt tttaaaaagg cgattgaaac
     4501 atgaggttga ctagcttcca cactggcttc aatagcattg tcacaaagga tagacacaat
     4561 ggttagaaaa tcaagtagac tcatcccctc gacctgaatc tcctcaggaa cttcgacatt
     4621 aaagacaatg ttcttatctc tggcttttaa aaatttccct gctagaagac ttttgagggc
     4681 tttatcacga atatttacca atctgcccag gtcatattta ttgttctgca atttctgact
     4741 ggaatccttt aagacggagc catagacctc ttttatctgc tccatatcct cctcttcaat
     4801 gcccagacgt aagctagtca agaggttggt ataatcatga cgaaagctcc gtacttcctt
     4861 gtaaagctcc tctatatgcc gactatagcg ttccatatct ctatagcgca gggcctgctc
     4921 ttgttccaat ctctcataga gtttttcctt caaataggta tccaatttct tgataacccc
     4981 cataaaaaag agtaggtaaa agactaggat gagatggcga acagtctttg attgaatact
     5041 ttgttcatat tcaaaaaaag acagactttc catgactaga tagtagccac ccattatcca
     5101 gttaatctga gtcagggact tttgaaaggc tttatcgaga atctcctttc tcaagctagt
     5161 aaaatcgtag tccaaccatt tcaaaaaagc tagagaaatg aagaaattga aaattattat
     5221 acataaccca gtaaatgagt agccatcata tacttgccct tgtcccaaaa atggaagcac
     5281 aaaataggag actcctctat aaaagagatt caccaatatc attggaaaga gaccataaaa
     5341 gaaaaggagt tttttaggaa gccctctcaa taataagaaa gataagccta tgccgtacaa
     5401 gggttccata aaataagata ggtaaacatt tcctaccata tagccaatca tcacaaaaac
     5461 aaaggccaac agtatcttaa aaagaaaggc cttaaaaatc ctctcgaaag taagatcaat
     5521 tccatccacc ttaaagaaaa tgacaatttc tagtccatta gtaacaagtg tatacaacaa
     5581 tatccaagca atgttcataa attctcctag ctcagtgtaa tttattgatg gcctcagaca
     5641 cttccctgac cttataacgg gcgattagac aacttccacc attgggaaag aaaagcagtt
     5701 tttctttctt atccaaatgc accacatttg caggattgat gagaaaagag cggtggcact
     5761 gcaagagacg gggctcctgc ttgaaaacct cctctaaact cgctgtaaat tccagcctgt
     5821 ctgtcttggt atagagaata acacgatggg ctctgggcga cgtttcgaga tagtaaacct
     5881 ctttaaaagg atactgaaat tgggcaaatt ttgatttaaa gtaaaagcaa tcttccgcca
     5941 gacttttact atcttgacta ttggcataga ggagggctgt ctcgatccga gattcaaact
     6001 cctctgctga caaggcctta tcaatgtagt ccaaagcaga cacttggtag cgaaaagaca
     6061 ggggcataaa ctccgagtga gtcgtcacaa agacaatcag ggcataagga tcccgatccc
     6121 gaatctttct agccacttcc agtcccttca tctcttcatt tcgaatctca atatccaaaa
     6181 agaatagctg atgggccccc ttctcatgca cttcagccag cagttggtcc ggcttgccaa
     6241 atacctcaaa agagctagga atgatatgat gtgctttcaa aagtttctca atcgtcgttt
     6301 caattctagt ctgttgggaa aaatcatcct ctaaaacaaa tattctcatc ttcttactct
     6361 ccctctttca accatttttg acgaatttct ctatagcgac gtctggcata cttgacagcg
     6421 tattgacctt cttctccgag aaaaaggatt ctttcttgaa aatcaatcga ttttaattta
     6481 tcaagattaa ccaaacaatt tcgatgacat ctcatcaagg tttccccaca ttggttctca
     6541 agattactta aattttgaag catattaaaa ctagcttctt ctgtaacaat ctgtacggta
     6601 tgggctttag ttggatgtgt ttggatatag tagatatcgt cgatgtttac tttatagact
     6661 gttttctgcg tctgaataat catatatttc attggattct cctcctaaga aaagaataac
     6721 agaaaaggtc tagcccaagc acacccaatc ctaaacagtc tttgaaaact cctaaaagga
     6781 ttattttcta caacatcatg atgagcttac aactatttta tcataaaatt cactcagcgc
     6841 aattcaagac atttcaatga caattaaaga tttgaataaa aaaaaagaat taaaagtgat
     6901 aaactaacag tatcaaaaca cagttgccag aagtaagact ggcacccaga ttaaaggagg
     6961 cattcccatg acagatacag accccatcaa aagagctcat actttgatta ctgacttaaa
     7021 caaagcctac caagcatgca aacaggcaag cgctgacgac gtccgctttc aggagcaatt
     7081 aaactctatt cttggttttc tagccaagac tgaaacagtg gataatcgat tcttgattga
     7141 actagaaaaa ttttaccaga cttccagtct tctcatggga ctcagcgctc ttgatccaga
     7201 tgctccaact cgcgccgctt ggcgggccta tgaccgcttc cactttgacc aagtcaagac
     7261 caagttaata ctcaatgaga atcaaagagc aaactaggaa gctagccgca agctgtactt
     7321 gagtacggta aggcgacgct gacgtggttt gaagagattt tcgaagagta taagtctcta
     7381 cggaccaacc attatcctgt agtaacaaaa aagaagctga gacaaactga actcaacttc
     7441 ttttttagtg tcgtatcagc aatgttagtc ctgcatctga cgttttacct gataaggcaa
     7501 atacaagact agtaaaacca aaccagctcc tagtaaggct agaagggcta gtttttcttg
     7561 gaaggtaatc aacccaacaa ctaattccac aagtaaaaca aaactggtca atcctcgaac
     7621 tacctcctgt aagccttttt ccttttgccc cttgtccagc ggaaagagtt gggtcaaata
     7681 ctggtagtca aaggcatgat agagggccag caactggaag agcaagaggt agttaaagag
     7741 aactaccact gctgtcgcaa tccaagcttg ctcgataaaa acctgcgcca gtacggaaag
     7801 taagagaaga cgaagactga gagcaaagag gtcgccattt cgcagataag aacgcagata
     7861 gagattttgc caaatcttcc caggcacctt ctgaacagcc tttaagataa agtccagata
     7921 ggcacgacgc ttgacactgt ttgaaattcc cttgacctgc gtaaagaggg caaagaaacg
     7981 aagcaagact tgcttacgct tgctttcttg agaaataaga tagtcccagt ccagtccagt
     8041 ttcagtgaaa aatttgctgg ccttttgacg aaagtggaaa tattttccta cccccaataa
     8101 aagcacatag agcagaaaaa ctggcaagcc ataacccatt gctaaaaaca agggcgcaaa
     8161 taacagcaag aaaagggtct gtacaaagag ccaaaagact agggaaatgc cagtttgacg
     8221 cttgagatgg agcttgattt cctcttctcc aactaagaga aagagcttgt ctggagcctc
     8281 catataggtg gcaattcctc cccaaagtaa aagtaaaaca gacgtaattc ctacaaacaa
     8341 aagaataggc caatgatttt caggaaaatg ttgtaagagt tgactgtact ggtaggctag
     8401 aaagcccaac aggacaagca ggaataagac aaagtggtca ttgagcacat agcgcagata
     8461 accaagacac tccttacgaa aggcctgctt tctctttaaa aacaagtctt tcataggtcc
     8521 tcctcttttg tcagagccaa gtaaatatca ttcaaactag cctcaggcat atcaaaggct
     8581 tcgcgtagtt gcaggagatt gcctttggca cgcacctctc ccttgtgaag aatgacaaag
     8641 gcatcacaca tcttttccgc cgaatccagc acgtgggtac tcatgagaat agacttgccc
     8701 ttttgcttct ccacttccaa aagctgaatc aaatcagaaa tagccagcgg atcaagacca
     8761 aggaaaggct catccacgat gaaaagactt ggatccacca caaaagcaca gataatcatg
     8821 accttctgct tcatcccttt tgaaaaatga acagggaacc agtctaattt ctgttccaga
     8881 cggaacatct ttaacaaggg ctctactcgt tcgaaagcca ctttttgctc aataccataa
     8941 gccatagcaa ccgtttcgat atgctctctg agggtcaatt cctcatacag actaggcgtc
     9001 tcaggaatgt agccaatctg cttgcggtag ctagtcgcat ctccttgcag agtcaggcca
     9061 ttgatattga tggagccact ataaggtgcc aacagaccga taatctcatt gatcgtcgtt
     9121 gatttcccag caccattgag accaatcaaa ccgaccaact gcccactttc aacagtaaag
     9181 gacacatctt tcaaaacagg aacatgaaca tagccacctg tcaggttttt aatttctaac
     9241 atattttctc caaatctggt ataatgtagc tatattatat caaaattcag tacagtagag
     9301 gtagatttta tgtcagattg cattttttgt aaaatcatcg caggggaaat tcctgcttca
     9361 aaagtatatg aagatgagca ggtccttgcc tttcttgata tctctcaagt aacactagga
     9421 cacaccttgg tcgtgccaaa agaacactat cgcaatcttt tggagatgaa tgctacgagc
     9481 gccagccaac tctttgccca agtaccaaaa gtagctcaaa aagtcatgaa agtcactaag
     9541 gctgctggta tgaatatcat ttccaactgt gaagaagtcg ctggtcaaac agtttttcat
     9601 actcacgttc accttgtgcc tcgctacagt gctgacgatg acctcaagat tgattttatc
     9661 gcccacgaac cagactttga caaacttgct caagtcgctg aaaccatcaa aaacgcttaa
     9721 ggagttgcta tgaaattatc caacctactg ctatttgcag gagctgcagc cggaagttat
     9781 ctggttacaa aaaatcgcca aaccatcaca gatgaagtct taaataccac tgaccgcgtt
     9841 caagctatca aagacgatgt ggatattatc caaaatagcc tgcaaatcat cgaccagcaa
     9901 aaagaactta tcaaggaata ccaagaagac ttgacttaca agtttaaggt cttggaaaag
     9961 gatatccaga ctagactagc tgtgataaaa gaaatgcagg gaactgaaga taagtaaaaa
    10021 gagcccccgg ctctttttta aaacgtaaac aagttatagt gaattgaatt tagaatagca
    10081 caccatagtt tctaaagcat tgttagagat tactttaaaa tcctcttatc aatttgttca
    10141 tattctattt caatctatta tatatatccc atcaactatg catcataatt taggtaactc
    10201 atactcaata aaaatcaaaa agcaaactag aaagctaggc acagactgct caaaacaccg
    10261 ttttaaggtt gtggatagaa ctgacgaagt cagtaaccat acccatgata agccgacgct
    10321 gacgtggttt gaagaaattt ttgaagagta tcattctcca tcaaaggcat cttttatatg
    10381 gtcaaagaag ccttttttct ttggatttac tttcaagtca ccagcagccg cgaattcttt
    10441 caaggctact ttttggcggt cgttcaagcc tgtcggtgtt acgacattaa cagtaacgta
    10501 ttggtcacca actgcaccgc cacgaaggct cggtgccccc ttactacgta ggcggaattt
    10561 cttaccagtc tgagttccct ctggaataac caattcaaca tcaccgtgaa cagttggaat
    10621 atctactgta tcaccaagag ccgcttggac aaagttgagg ttgagattgt agaagatagt
    10681 cgttccttca cgttcaaact tgtcgctagc ttctacagaa actactacat acaagtcacc
    10741 ataaggtcca ccgttaaagc ctgcttcacc ttgaccagcg aggcgaattt gttgacctgt
    10801 ttccacacca gcagggattt tcacatgtac gctatgagct tgtttctcat gacctgttcc
    10861 atgacaggtt gtacatggat atttgatttc ttttccttgg ccgtgacaga catcacaggt
    10921 tacttggcga cgcatcatac caagaggagt ctgcgtatcg acgttaatga caccagcgcc
    10981 atgacagcgt ccacaagtga ctggacttgt ccctggctta gcaccagagc cattacatgt
    11041 acgacagcca gcttcacgat gatacttaac ttccttctca gttccgaaga tagcttcttc
    11101 aaaggtcaaa ttgacacgat actggagatc atctccttgg cgaggagcgt ttggattgcg
    11161 cgaagaaccg cctccgccga agaaacttga gaaaatatcc tcaaaaccac cgaagccacc
    11221 tgccccattg aaaccgccga aaccaccagc tccaccaaaa ccaccattgg cgcctgcagc
    11281 accatactgg tcataggcag cacgtttttg gtcgtcactc aaagtctcat aggcttcttg
    11341 aacttccttg tacttgtcct cagcaccagg ctccttgttg atatctgggt gatatttttt
    11401 ggaaagctta cgataagcct ttttgatttc gtctgccgaa gcgtttttgg atacccccag
    11461 acgatcataa aattcagtat tgttcataat tcaagataca aagggcgtca aacggacaca
    11521 gccatctagg agttttgacg acggacgctt atgtcctaga aaaaactatc tttttggcac
    11581 agtccgtagc ccgtgttcag ttacgaacac ccttattcct ttctattgat gttctgaatc
    11641 gaaccaccat ttaggtcggg ttcaattcct ttcttttata tttagtaaga aattctgaaa
    11701 cccgtgttca gttacgaaca ctctttgttc ctttctattg atgagacaaa acccaaacaa
    11761 atgagattcc gtcccttttt atcgaaaaac agaggctggg ttgcaacctc tgcaaataag
    11821 taaattaact ttattttgat tttaaatatc ctataagtac agaaatattt atatattttt
    11881 gtctcttctt agtatttgcc tatataattc caattgatca taagaaatac aactactgtc
    11941 caaatcatac caattatgaa cggtattaca aatttatatg tttgtatttc taatgatatc
    12001 atagctgata ccatcgccga tacaaatgca gacaacaata ctgaggtaac aaaattcctt
    12061 accgcttcat catttttgat acaactatca tgttcagcta atataatttc tctatatcta
    12121 gtcaaacatt ctactggaat attatctata aaaccttttg ctgcttcata gttgtcagct
    12181 tgattacaaa aatcattata aacttttcgt gtatttttta gatactcatc atccaataca
    12241 ctcatcttac ttttccgtaa actctccgtc tacgacgtca tcgcctgcgt ttcctgttgc
    12301 ttgtgcgcct tctgctcctt cttgagcttg ttgcgctgct gcggcttgtt cgtagagttt
    12361 aacagcaagt ccttgagctt tttcgttcaa tgcttcaagt ttcgctttca tgtcgtccaa
    12421 gttgttgtct tcttgagctt tcttaaggtc atcaagggca gcttgggcag cgtcacgttc
    12481 tgcgtcgaag cctttacctt cagtttcctt gattgtcttt tcagtcgcaa agattgcttg
    12541 gtctacttca ttacgaaggt ctacttcttc tttacgtttc ttatcggctt cagcgtttgc
    12601 ttctgcatct ttcatcatgc ggtcgatttc ttcgtcagtc aaacctgagt tcgattggat
    12661 gacaatagtt tgttcttttt gagttccaag gtctttggcc ttaacagaca cgataccgtt
    12721 cttgtcgatg tcaaatgtta cttcgatttg aggaattcca cgaggtgcag ctgggatatc
    12781 agtcaattgg aagcgtccaa gagtcttgtt atctgctgcc attgggcgtt caccttgaag
    12841 aacgtggata tcaacggctg gttggttgtc tgctgctgtt gagaagactt gtgatttaga
    12901 tgttgggatt gtagtgttgc gatcgataag ttttgtaaat actccaccca ttgtttcgat
    12961 accaagtgac aatggcgtta catcaagaag gacaacgtcc ttgacatcac cagtaatcac
    13021 accaccttgg atagccgcac ccatagcaac tacttcatca gggtttactg atttgtttgg
    13081 ttctttacca gtttcagctt taacagcttc aacaacggca gggatacgag ttgaaccacc
    13141 aacaaggata acttcgtcga tttctgacaa gctcaaacct gcatctgaaa gggcttgacg
    13201 aactggaact tttgtacgtt caacaaggtc acgagtcaaa tcatcaaatt tcgcacgagt
    13261 caaagtcatt tccaagtgaa gaggtccagc ctcacctgca gtgataaatg gcaagctgat
    13321 ttgtgttgaa gttacaccag aaaggtcttt cttcgctttt tcagccgcat ctttcaaacg
    13381 ttgcattgcc atcttgtcag tagacaagtc gataccgttt tctttcttga attctgctac
    13441 caagtggtca atgatttttt ggtcaaagtc gtcaccacca agtttgttgt cccctgcagt
    13501 tgacaatacg tcgaagacac cgtcacccaa ttcaaggata gagacgtcga atgtaccacc
    13561 accaaggtca aataccaaga ttttttcttc tttatcagtc ttgtccaaac cataagcaag
    13621 agctgctgca gttggttcgt taacaatacg ttctacttca agaccagcaa ttttaccagc
    13681 gtcttttgtt gcttgacgtt gagcgtcgtt gaagtaagcc ggaactgtga taacagcttt
    13741 ggttactttc tcaccaaggt agtcttcagc gtagcctttc aagtattgaa ggatcatagc
    13801 tgagatttct tgtggagtgt attcttttcc atttgcagaa actttttcag aagttcccat
    13861 cttagatttg atagagataa ctgtatctgg gtttgtgact gcttgacgtt ttgcagcatc
    13921 accaacgatg atttctccgt ttttgaatga gactacagat ggagttgtgc ggtttccttc
    13981 tgggtttgcg atgattttgc tttcagttcc ttcaagaact gcaactgctg agtttgttgt
    14041 acctaagtca ataccgataa ttttagacat gtgtttttct ccttgttagt taattttcta
    14101 tttttctttt tgatactctt cttaagtggc gacgccgtca gagcttgact tcgtcaatct
    14161 ctttgactaa actttgagcc taaggtctca aaatttgcgc aaatagcgcc acggcgaaga
    14221 gtatcgtgtt tggttcgctt cgctcactat tacaaagcca aacatatttt atctttcttc
    14281 atagtttatt gtcgtttcgg acaagttttc tattatgtaa attgcgacac gaagagtcga
    14341 aatcgatttt atttcgacga cgagttagta aggaagctag gcaaacgcca tagcgattgc
    14401 cgttttctga cgagttgctt tagctaccgt cagaattgcc gaatcgaaca cgccctaagc
    14461 tctgagtaaa aaagatagat tttcttgtgt tcatcgaaca cttcgttaat ctcctatttt
    14521 tactgcgagc tttttacggg ctttgtatct tagttataca ccactaccat tgctgggcgt
    14581 aggatgcggt catggagttt gtagcctttt tgaaagactt gagcgatggt atctactggg
    14641 tgttcatcgt ctgctgggag agtttgtatg gccatatggt agttatggtc aaattcgcca
    14701 tctgctgcga tttcttcaat tccttcttct ttcaaagcgt gaatcaagct ttcttgcacc
    14761 atccccaagc ccttcttcac atcatctgtc aaaccttcaa ctgcaagtgc acgctcaagg
    14821 ttgtcaagag atggtaagat tgcttttgcc aagtcctggc tacgataacg ttgcaagttt
    14881 tgacgttctt cattggcacg gcgttggata ttttgcattt ctgcatgagc gcgaagatat
    14941 ttgttttcga actcatctgc acgttcattt gccaagtcca acttagactt ttcaggagtt
    15001 gtttcttcag ctgtttccac aacttcctct tcttgaactt cttctacttc ttctacttct
    15061 tcatttttta tatcttgggc catgttcgcc tcctttaatg atttcaatct taatgtactt
    15121 cgtaatgatt actgctgagg tagcggtaaa aatctgtcaa cttcatagtc aaaacacggt
    15181 tgaccacatt gacttgattg attagctgtt ggtaatccag attaactgga ccgataatgg
    15241 ctagaattcc aactcctcga taaggaatga ggaacttact actaatcacc gctaggtcag
    15301 ctaaacagga ctcttgaccg tctgcaatac gaacattttg catctgatcc tcacgcaacc
    15361 cctcacgaat ctccaaggcc accttttgcg gttggtcaaa gaactgatag gctgctagat
    15421 tggcaaaatt caagagattg accttgcccg ccatcacaat gttttcgttg aacatttcct
    15481 taaagatgtg ttcaaagaga tcgatgacat tatccgttgt tgtaaagtaa cgctggataa
    15541 tctgcggaat ctccgtccga atcttgtagt gaatatctaa aacggtgtga ccgaggaaac
    15601 gttcctgaat gatgctcttc agtttcagca aatcctcctg caagaagttc cttggaatca
    15661 gaaactgact agtaaccgtt cgcgactcgt ctagggtaaa taccgccaag gctgtatgtt
    15721 gccccaaaac aacgatatca aaggctgtca aacgttgcct gctcggctca acatccagtg
    15781 ccactaccgt acagccactc aggtctgtta gcaagttagc agcctcttgc agaatatcct
    15841 ccaatttgaa gaattcctga tcaaaggctt tgacaatctc atatacctca ttttcagcca
    15901 gccggtgaaa atccagtgag tgtttcacat agtactgaaa accagcaaca cttggcatcc
    15961 gaccgcttga agtatgagcc ttctcaagca acccttgttt ttctagttcc gccatgtcat
    16021 tacgaatggt tgcactgcta gagttaatag actcttgcaa ggcttttgat ccgacaggtt
    16081 cgtgcgtttt ggtaaagatg tcaataatca gatttaaaat atcctgctga cgctctgtaa
    16141 ccatctcatc actcctctct gtctgatcaa ttagcactca atgcatccaa gtgctaactt
    16201 atgattctat tatacaccct tagaagagaa tgtcaagaca aaaactcaaa aaattagcac
    16261 tcttttatca agagtgctaa aaatcaatct gaagacctag aaccttgcta ttcatctact
    16321 cggtatttcc tttttagtct gaaataacca tgaattagat aagaaatcag aaaagcatat
    16381 aaaacaaaaa taagaaagaa agacatagaa agatcctctc taaacaaact cgtacaaaca
    16441 acaagaccac ctaagaaagt agttgtacag gaacgaagtc tagatcgcat aacttcccat
    16501 ttgagcccct tactcatata gacttgatcc tctggaagta aatcagaaga cgatttacga
    16561 agaatcgaac aagaacctgc tcctatcaat tcccaacctc tatctctaaa atcttgcagt
    16621 tcatgcttat attttttaag aaatctagaa tcatagatac ggtagatgac atcgtctggt
    16681 tgacattggt caaaatagaa caaaccaaaa cgactcgttc tatacctcca acctttcaaa
    16741 tgcatctcat gtaaatattc ttcttccttg tccaaatcaa caatagtgaa aatccgaaat
    16801 tctactctgc tattcattgt cttaccccaa aattagaaaa catgcctggc gttatttatt
    16861 agataattct ttccgctttt gactcaatct ccaaaaaata taagaaatct gaatcgcaaa
    16921 aactatcaat aaaacccaat ctattatgaa agtcaaaaac acttcccaac tgaaagaact
    16981 acctccagtg acaaactttg agaaaaacgg tagtagagct aaaaagagaa gcaaaatagg
    17041 aagcatccgc attgttaaaa tccttttggc ataaaaaaat ctttatttaa acgaaaatat
    17101 tatggcaaaa tttacgccag tttttgaacg gctgatgtag atattttata ctttcaaaat
    17161 gtttaaatgt gattatttat ttttgaaaaa tagctcacca gcccgactga aagtgcttat
    17221 aaatgataat aagtcgcctg ccgaaaacag cgaaaaatag cggtgttatg cggagataat
    17281 ctgacgcgat gcgaaagtat attgcatact tattttcaac aatttagcag agtattttta
    17341 taagtgtgat ataatagaag tataatttgt tctgatagtt tattttatgg agaagtagat
    17401 ttttagaatg cggagggttc aatatggttg agtttataaa gtctaagaaa gaaatgagtg
    17461 aggaggatat taaagcaaat ttcatcactc ctgctattgt atccaaagga tggaaaaatg
    17521 gtgagcatat cgcttacgaa gaatacttca ctgatggtcg aattgaggtt agaggagata
    17581 aggctcgtcg taaagaagga aaaaaatcag actattcact gtattaccaa tttggaactc
    17641 gaattgcaat tgttgaggca aaggataata aacacagcgt tcgagcagga ttacaacaag
    17701 ctattgaata tggagagatt ttagatgttc catttgttta ttcttcgaat ggtgatggct
    17761 ttattgaaca cgaccgtatc acgagagaag aacgtgagct ggagttagat gaattcccta
    17821 ctcgtgaaga attattttct cgtatgacga aggaaaaagg attgacgtac gaaattacag
    17881 aagctatctc aactccatac tatacagacg ccttctcaat gaaaacgcca cgctattatc
    17941 agcaaatagc tatcaaccgt actattgaaa cagttgccag aggacaaaaa cgagtaatgt
    18001 ttgtgatggc aacaggaacg gggaaaacgt tcatggcttt tcaaattatt catcgccttc
    18061 gaaaagctgg tttggctaaa cgagttttat tcttagcgga tagaaacatc ttagtagacc
    18121 aaacgatggc tgaagacttt aggccattcg aaaaggtaat gacgaaaatt acaccaaaac
    18181 ttttgactgc tcctgaaaaa ttaaattctt ttgaaattta tctagggctt tatcagcaac
    18241 taactggtga agatggaact gaaacacatt atcaaaaatt tgacaaagac ttctttgatt
    18301 taatcgtaat tgatgaagcg caccgtggtt cagctaagga aaacagtaac tggcgtaaga
    18361 taattgatta tttcagttct gcgacacaga ttgggatgac cgctactcct aaagaaacca
    18421 agaatgcttc caatacggaa tactttggtg agccaatcta tacttatagt ttaaaacagg
    18481 gaatcgagga tggttttttg gctccatatc gtgttatgag agttaattta gatgtagatg
    18541 tggatggtta tcgtccagaa actggaaaag ttgatgctaa cggacaatta atagaagata
    18601 ggtactacgg caggaaagat tttgataaaa ccattgtcat tgatgataga acgcaaagag
    18661 ttgccaagtt tgtttctgat tatatgaagc aaaacaatgc acgatttgat aaaacaattg
    18721 ttttttgtgt tgatattgac catgccgagc gaatgcgtgc tgcacttgta aaagagaatc
    18781 tagacttagt ccaagaagac tatcgttatg tcatgcaagt aactggtgac aacgctgaag
    18841 gaaaagctca actggataac tttatggatg tcaattctaa ttttcccgct attgtaacaa
    18901 cgtctaaatt attaacgaca ggagttaatg ctaaaacatg tcgtttgatt gttttagact
    18961 ctaatatcca atccatgact gaatttaaac aaattattgg tcgtggcaca cgtctttatc
    19021 ctcaaaaggg gaaagaattt tttacgatta ttgattttcg aaatgttacc aatttgtttg
    19081 ctgaccctga ttttgatggt gatccagtga aggtgctgga aacaggtgcg aaaacagtca
    19141 gtggttctac gcccggtttc gtaagtgagg aaggtgaccc agtagaaaaa tatatcgtta
    19201 cagacaagca ggttaccatt cttaattcta ctgttcaagt attggatgaa aacgggaaac
    19261 tgattaccga aagcctgacc gactacactc ggaagaatat cttaggtagc tacgccactt
    19321 tgaacgattt tatcacagtt tggcatacgg cggataaaaa gaagcttatc ttagacgaac
    19381 tttataaaaa aggagtttat ctagatgcta ttcgagagtc ggagggaata tcagaacaag
    19441 aaatcgatga ttttgattta ctcctaaaac ttgcctatgg tcaaaaagaa ttaaccaaaa
    19501 cggaacgtat caataagctc aaacaaagcg gatatttata taaatatagt gaggaagcgc
    19561 gtgctgtttt ggaaatttta ctgaacaaat acatggataa aggtattgga gaactcgaaa
    19621 gcattgaaac attaaaactt ccagaatttc agatatatgg tggaaccttc aaaatcatca
    19681 atacttattt tggagataaa aaacgatatt tacaagcaat taaagaattg gagcaagagc
    19741 tatttacagt agcttaatga aaggaaagta tgtcaattac atcatttgta aaaagaattc
    19801 aagatatcac tcgaaacgat gctggtgtta atggtgatgc tcaacgtatt gagcaaatgt
    19861 cttggttatt attcttaaaa atttatgata gccgtgaaat ggtttgggaa ttagaagaag
    19921 acgagtatga gtcaattatc ccagaggaat taaaatggcg aaattgggct catgctcaaa
    19981 atggggaacg ggtattgaca ggcgatgaat tacttgattt tgtcaataac aagttattca
    20041 aagagttgaa agagcttgaa ataacttcaa atatgcctat tcgaaaaacg attgttaaat
    20101 cagcttttga agatgcgaac aactatatga aaaatggcgt cttgttacgc caagtcatca
    20161 atgttattga tgaagttgat ttcaatagcc ctgaagatcg tcattcgttt aatgatattt
    20221 acgaaaaaat tcttaaagat attcaaaatg ctgggaactc aggagaattt tatacgccac
    20281 gtgcagcgac tgattttatt gccgaagttc ttgacccaaa acttggagaa tcaatggcag
    20341 accttgcttg cggaacagga ggcttcttga cttcgactct gaaccgttta agtagtcaac
    20401 gtaaaactag tgaagatacc aaaaaatata atacagctgt ttttggtatt gaaaagaaag
    20461 catttcctca tcttttagca gttacaaatc tgtttcttca cgaaattgat gaccctaaaa
    20521 ttgttcatgg aaatactttg gagaaaaatg ttcgtgaata tacggatgat gaaaaatttg
    20581 acattattat gatgaatcca ccttttggag ggtcagaatt agaaacaata aaaaataact
    20641 ttccagcaga attacggagt tctgaaacag ctgatttatt tatggctgtc attatgtatc
    20701 gtttgaaaga aaatggtcgt gttggagtta ttttacctga tggttttcta tttggtgaag
    20761 gtgtaaaaac tcgcttgaaa caaaaactgg tagatgagtt caacttacat acgattatta
    20821 ggttgcctca tagtgtcttt gcaccgtata caggaatcca tacgaacatt cttttctttg
    20881 ataaaacaaa gaaaacagaa gaaacttggt tttatcgttt agatatgcca gatggttata
    20941 aaaatttctc gaaaactaag ccgatgaagt cagaacactt caatcctgtt cgtgactggt
    21001 gggaaaatcg tgaagagatt ttggaaggta agttctacaa atctaaatca tttacaccta
    21061 gtgaattggc tgagttgaat tataatttag accagtgtgg ctttccaaaa gaggaagagg
    21121 aaatcttaaa tccctttgag ttgattcaga attatcaagc ggaaagagca actttaaatc
    21181 ataagattga taatgtatta gctgatattt tgcagttgtt ggaggacaaa taatgacacc
    21241 agaacaactt aaagcaagta ttctccaaag agcgatggaa gggaaattag tgccgcaaaa
    21301 tcccaatgac gaacctgcaa gtgaattatt aaagagaatt aaagctgaaa aagaaaaact
    21361 tatcagtgaa ggaaaaatca aacgagataa aaaggaaact gagatatttc gtggtgatga
    21421 tgggaaacat tatgggaagt ttgctgatgg aagcactcaa gaaattgatg ttccttatga
    21481 tattcctgat acttgggagt gggtgaggtt ttctacattg gttgaaattg tcagaggtgg
    21541 ctctccacga ccaatcaaag attatcttac ttctgaagta gatggaataa attggataaa
    21601 aataggtgat actgaaaagg gtgaaaagta tataaataat gttaaagaaa aaatcaaaaa
    21661 atcagggctt aacaaaacta gatttgtaaa aaaaggtaca tttttgttaa ctaattctat
    21721 gagttttggt agaccttata ttttgaatgt tgatggtgca atacacgatg gatggttggc
    21781 tatttcgaac tatgaaaact cattaaataa agattaccta ttctatattc tttcatcaaa
    21841 tgtagtttat tctcaatttc tatctctaat tagtggagct gttgtgaaaa acttgaatag
    21901 tgataaagtt gcttctattc ttatccctct ccccccacta tccgaacaac aacgcataat
    21961 agaagcaatc gaatcagctt tagaaaaagt agatgaatat gctgaaagtt ataatagact
    22021 agaacagcta gataaagaat ttccagataa actaaaaaaa tctattcttc aatatgctat
    22081 gcaaggaaaa ttagttgaac aagacccaaa tgatgaatca gtcgaagttt tacttgaaaa
    22141 aatacgagca gaaaaacaaa aactctttga agaaggcaag attaaaaaga aagatttgga
    22201 catttctatt gtttcccaag gagatgataa ctcttattat gaggaagtac cttgtgaaat
    22261 accagaaagt tgggaatggg tgaggttaaa cgatataacc tcctacattc aaagagggaa
    22321 gtctccaaaa tattcaaata ttcctatata ccctgtgatt gctcagaaat gtaatcaatg
    22381 gtctggcttt tctattgact tagcaagatt tattgatccc gaaacagttc atagttacca
    22441 gaaagaaaga ttactaagag atggtgattt aatgtggaat tcaacaggtc taggtacgtt
    22501 aggacgatta gctatttacc atgaaaataa aaatccttat ggctgggctg ttgcagatag
    22561 ccatgttaca gttataagag tcttatcggg agttattaat tgccatttca tctataattt
    22621 tctttcctct cctattgttc agtcagttat tgaagaaaaa gcgtcgggta gtacaaaaca
    22681 aaaagaatta ttaacgaaaa ctataaaaga atatttaatc cccctcccac cccttcctga
    22741 acaatccaga atcgttgata aaatcgaaca atttttcgcc catattgacg cactaattta
    22801 gtataatagt tttacgtgcg gtggaatttc tatcaaacat aagataggag ttttacgttg
    22861 aaagattttg aattacattt gaagaaagct ggtttagctg aaaatactgt gcgttcgtac
    22921 ctttatggtg ttcgattttt cttggagaat tacgaactca aaatggaaga tttatttgaa
    22981 tacaaaggat atttattaga caattttaaa cctaaaactg tcaatttgcg actacaagga
    23041 gtaaataaat atctggcttt tattggtcat gatgatttga aattaaaatt tgtcaaagta
    23101 cagcaaaaac cgtttttaga agatgtcatt agccatgctg attacctttt tcttaaacgt
    23161 agtttgaaaa aagatggtat tttaaaatgg cattttgtcg tttggttttt aggtgcaact
    23221 ggggctcgtg taagtgaact tattaaacta aaagtagagc atgtagaaat cggttatttt
    23281 gacatttact caaaaggtgg taaaattcgt agactgtaca ttcctaaaaa attaagaaat
    23341 agttgtctta gttggttaga atcagaaaat cgccgaagtg gttatttatt tttgaataaa
    23401 tttaacgaac caattacagc aagaggagtt gctcaacagt taaaaaatta tgctgataaa
    23461 tacaaaatga atcctaaagt aatttaccct cattctttta ggcatttatt tgctaagaat
    23521 tttttagcga agtataatga tattgccttg cttgcagatt tgatgggaca cgaaagtata
    23581 gaaactactc gaatttatct aaggaaaaca gctactgaac aacaaaatat tgtagataaa
    23641 attgttaatt ggtaaaaaat aacaggtggt caaactgact acctgctatt tttgtgatta
    23701 tggctcttat tatgggaata aagacgaaac aacctcttat cctatatatg aaatcccaga
    23761 agcatggaga tatattaaat ttgctagcct agttaacttc agaataggaa aaactcctcc
    23821 acgcagtgaa gctacttttt ggggaactga aataccttgg gtatctatat cagatatgcc
    23881 tatatctggt tatgtaacta atacgagaga atctatttct aaattagcat taaaatcaaa
    23941 gaaaatagac atttcaccta aaggaacttt attaatgagt tttaaattat ctattggaaa
    24001 agttgccata ttggatattc ctgccactca taatgaagcc attatatcga tattccccta
    24061 tgctaataaa gaaaatatta tcagagatta cttaatgata tttttgccac ttatctctac
    24121 tttaggtgat tcaaaagatg ctatcaaagg gaaaacatta aatagtacca gtatctccga
    24181 attattaatc cctatttcta atcatgaaga gatgaaaaga atcatttcca aagttgattt
    24241 actttttcaa aaagtttctc aactttttga gtaataagtt cctgttcctc aaaaggagct
    24301 aacggaatta atagctcgct cagtgtagtt ttaggaatat tatataaagc ttgacctgat
    24361 agtttagtta ttgctttcaa ttgtttataa aataacggag aagacaagtt aaataataga
    24421 aattttgaaa taatctctga actttcgaat ggtgttaatt ggaaaataaa tccaccagcc
    24481 acaacaccat catagtcttt atcgattctt gcaaactttc caatatgttc taaagaggtt
    24541 gatacaggtg ttattagctg attatgtttt aaataaactt gttcagagga gatgaattgt
    24601 gtatcaatgt agtaatcgtt atccaacaga gaaaattcta aaggcttaat attaccacca
    24661 cgtataattc taacaccttt attattaatg cttaaatcgc ccttcttgta agaaagacct
    24721 gtattcattg aaaaaatatc ttttattttt ataacaaccc aattcatagg t
//
LOCUS       DAVKWZ010000029        23838 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000029 DAVKWZ010000000
VERSION     DAVKWZ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23838
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.029"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(387..1583)
                     /gene="tuf"
                     /locus_tag="VW854_001205"
     CDS             complement(387..1583)
                     /gene="tuf"
                     /locus_tag="VW854_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV0262441.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(2494..3363)
                     /locus_tag="VW854_001206"
     CDS             complement(2494..3363)
                     /locus_tag="VW854_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV0262442.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTENPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLSIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(3586..4263)
                     /locus_tag="VW854_001207"
     CDS             complement(3586..4263)
                     /locus_tag="VW854_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV0262443.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVVSGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPEKPEIPSPQEPGTPGEPTPEKPIPQPNPEHPSVPTPNPELPNQETPIPEP
                     TPEPGTPKTETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLAGLGLGFF
                     KKKEDEK"
     gene            complement(4235..5326)
                     /locus_tag="VW854_001208"
     CDS             complement(4235..5326)
                     /locus_tag="VW854_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEV0262444.1"
                     /translation="MNIILIAKPLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEVDTNSASLTGNIVSKGRTVTLVYRESEAPTTATVTANY
                     YKEGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTIITVRMLRKSGSQNSNLY"
     gene            complement(5437..5691)
                     /locus_tag="VW854_001209"
     CDS             complement(5437..5691)
                     /locus_tag="VW854_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262445.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRITNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRAYKLLKTLIPGENYLSC"
     gene            complement(6031..6477)
                     /locus_tag="VW854_001210"
     CDS             complement(6031..6477)
                     /locus_tag="VW854_001210"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0262446.1"
                     /translation="MDETGIDRYLYRPYARAPRGEKVYEKISGRRFERTSIVAGQVDG
                     EFIAPMIYKKSMTSDFFVEWFKTQLLPALKTPHVIVMGNASFHPKNILDELCIQDKHF
                     FLPLPPYSPDLNPIEQAWAILKKKVTDVLREVSTIFECLECFFKTR"
     gene            complement(6550..6876)
                     /locus_tag="VW854_001211"
     CDS             complement(6550..6876)
                     /locus_tag="VW854_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0262447.1"
                     /translation="MAYNTDFKQGALDSIKGGHRHVEAAKVFWCWRQNSLHVGKERRE
                     QRNLERKKRVVKKRKIPLEELKAFVEAHPDAFLREIAAHFDCAVPSVWAALKQIKVTL
                     KKDDDL"
     gene            7110..8828
                     /locus_tag="VW854_001212"
     CDS             7110..8828
                     /locus_tag="VW854_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEV0262448.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDVEYLKEVKDVNI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPKEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            8939..9286
                     /locus_tag="VW854_001213"
     CDS             8939..9286
                     /locus_tag="VW854_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV0262449.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFVHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(9565..10401)
                     /locus_tag="VW854_001214"
     CDS             complement(9565..10401)
                     /locus_tag="VW854_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV0262450.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(10414..11043)
                     /locus_tag="VW854_001215"
     CDS             complement(10414..11043)
                     /locus_tag="VW854_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV0262451.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(11053..11694)
                     /locus_tag="VW854_001216"
     CDS             complement(11053..11694)
                     /locus_tag="VW854_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV0262452.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(11840..13069)
                     /locus_tag="VW854_001217"
     CDS             complement(11840..13069)
                     /locus_tag="VW854_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV0262453.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLAL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLMLGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLAAKEDAEDTEEILLRLATIYLKQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(13059..14225)
                     /locus_tag="VW854_001218"
     CDS             complement(13059..14225)
                     /locus_tag="VW854_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV0262454.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            14369..15382
                     /locus_tag="VW854_001219"
     CDS             14369..15382
                     /locus_tag="VW854_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEV0262455.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(15427..15846)
                     /locus_tag="VW854_001220"
     CDS             complement(15427..15846)
                     /locus_tag="VW854_001220"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEV0262456.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(15857..17263)
                     /gene="atpD"
                     /locus_tag="VW854_001221"
     CDS             complement(15857..17263)
                     /gene="atpD"
                     /locus_tag="VW854_001221"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEV0262457.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFAGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            complement(17349..18227)
                     /locus_tag="VW854_001222"
     CDS             complement(17349..18227)
                     /locus_tag="VW854_001222"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEV0262458.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(18243..19748)
                     /locus_tag="VW854_001223"
     CDS             complement(18243..19748)
                     /locus_tag="VW854_001223"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEV0262459.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(19763..20299)
                     /locus_tag="VW854_001224"
     CDS             complement(19763..20299)
                     /locus_tag="VW854_001224"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEV0262460.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(20299..20793)
                     /gene="atpF"
                     /locus_tag="VW854_001225"
     CDS             complement(20299..20793)
                     /gene="atpF"
                     /locus_tag="VW854_001225"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEV0262461.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(20807..21523)
                     /gene="atpB"
                     /locus_tag="VW854_001226"
     CDS             complement(20807..21523)
                     /gene="atpB"
                     /locus_tag="VW854_001226"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEV0262462.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(21558..21758)
                     /locus_tag="VW854_001227"
     CDS             complement(21558..21758)
                     /locus_tag="VW854_001227"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEV0262463.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            complement(21833..22786)
                     /locus_tag="VW854_001228"
                     /pseudo
     CDS             complement(21833..22786)
                     /locus_tag="VW854_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155340.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(23217..23615)
                     /locus_tag="VW854_001229"
     CDS             complement(23217..23615)
                     /locus_tag="VW854_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0262464.1"
                     /translation="MENKESYKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     GFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
BASE COUNT         7072 a         5093 c         4337 g         7336 t
ORIGIN      
        1 catattacta ttgtataatg ctttttgatt ttagtctagt attatactct tcgaaaatct
       61 cttcaaaccg cgtcagcttc gccttgtcgt atagatgtta ctgacttcgt cagttctatc
      121 tacaacctaa aaacagtgtt ttgagcagtc tgcagctaac tttctataac aaaatgaaat
      181 aagaacaaga caaatcgatc aggacagtca aatcgatttc taacaatgtt ttagaagtag
      241 atgtgtacta ttctagtttc aatatattat agtttgctct ttgattttca ttgagtatta
      301 gttcaatatc taaaaaaaga accttgccaa gcaagattct tttagtgtct gacttaaata
      361 attgttcttc tgggaactaa atcgaattaa gcttcgattt ctgtaaccat acctgaacca
      421 acagtacgtc caccctcacg gatagagaat gtagtacctt gttctacggc gattgggtga
      481 atcaactcaa cgtcgattgt cacgttatca ccaggcatta ccatttcagt acctgctgga
      541 agttcgattg aacctgtaac gtcagtagta cggaagtaga attgtggacg gtagttgttg
      601 aagaatggag tgtgacgtcc accttcttct ttagtaagga tgtagacttc acctttgaat
      661 ttagtgtgtg ggttgattga acctggttta gcgataactt gtccacgttc gatttcatca
      721 cgttgaacac cacgaagaag gacacctacg ttatctccag caagaccttc gtcaagttgt
      781 ttacggaaca tttcaacacc agtaacaact gctttttgag tttcttcttt gataccaacg
      841 atttcgattt cgtcgttgac tttaacgata ccacggtcga tacgtcctga agcaactgta
      901 ccacgtccag tgattgagaa tacgtcctcg actggaagaa gcaatggttt gtcagtgtca
      961 cgttctggtt ctgggatata ctcatcaact gtgttcatca attccataac gatgtcttcg
     1021 tatttagagt caccttcaag agctttaagt gctgaacctt ggataactgg aagatcgtca
     1081 cctgggaagt cgtattctga caataggtca cggatttcca tttcaaccaa ttcaagcaat
     1141 tcttcgtcgt caaccaagtc aactttgttc atgaagacga taaggtgttt aacaccaacc
     1201 tgacgtgaaa gaaggatgtg ctcacgagtt tgtggcattg gtccgtcagt tgaagctact
     1261 acaaggatag ctccgtccat ttgagcagca ccagtgatca tgtttttaac gtagtccgcg
     1321 tgtcctggag cgtcgatgtg agcgtagtga cgtttttcag tttcgtactc aacgtgcgca
     1381 gtgttgatag tgataccgcg ttcgcgttct tctggagcag catcgataga cgcatagtct
     1441 ttaggttggt taactgatga aggcaagcga cgtgccaaaa cagttgtgat agctgcagtt
     1501 agggtagttt taccgtggtc aacgtgtccg atagtaccaa tgttaacgtg tggtttacta
     1561 cgatcgtatt tttcttttgc catttgagta aaagcctcca ataaaatata ttttatagat
     1621 agacagtagg caatacagtc taactttcct tactatttta tcaaatttca gctaaattgc
     1681 aagtgtttta caatgttttt gtgaattatt cttggtaaaa atttaactaa ttaatagaaa
     1741 gtactaaata gacttatcct tcaaggagtt ttcagcatta tccatccact cacttgctga
     1801 aaatgtaaac agtctctggt ttatatagta aaatgaaaca agaatagagc aaatcgatca
     1861 ggacagtcaa atcgatttct aacaatgttt tagaagtaga tgtgtactat tctagtttca
     1921 atctactata caacctcaaa actgtgtttt gaacaacctg cggctagctt cttagtttgc
     1981 cctttgattt tcattgagta taaaattgca gataaaacga taacaatcat aggactcagc
     2041 agagtcaatg taggtcgaag aatagccccc ctaatcgctt attttatagc aaaatgaaat
     2101 agaaactgaa cattttgttg aggaaggtaa atcaattcct cacaatgttt gagaaagaag
     2161 gggggactat tccaacttca acccactata aagcagactt acaagatagg gaacccatct
     2221 ccgataatat atagtagaat gaaaactgca caatttgatt ggagaattca aaccaatttc
     2281 taacaatgtt ttagaaacaa gggtctatta tttcaactgc aacctactat agcccattga
     2341 agctggaata gcccactgag acttctaaac cactgctgga gaggagtttg tttagagttt
     2401 ctttcatttc cctaaaaaat caaaaagaga gaaccagtct gatcctctct tacttataga
     2461 tggtgctcac tcaaatgtta aaggagctat ttcttagaga taaaggaatt taaatacagc
     2521 tactgccgca attgctgctg cgataggtgc tactactggt acccaagaat accaccattt
     2581 tgaatcgcct ttatgctcac caagaactga ttttggaagg aaagcatgaa ggagacgtgg
     2641 tcccaagtca cgggctgggt tcaaggcagg tcctgtaggt cctccaagtg atgttaccaa
     2701 agccataacg aggaatccaa gtgccaagtg agccacagaa agtcctgaag cagtgtgtgg
     2761 agccacctgt gctttaatag acaagtcaga aaaatcaact gtttgtcctg cttctattgc
     2821 cttttgtttc atgaattgaa gcacttcagc accaaagaag tttttagtca aaccaagagc
     2881 tgcaaagaac aaaacaaatg aaccaacaaa ttcattaatc aaaccattga cagttgctgc
     2941 atagcgactt tcttttgtac catggtcaat acttgaaata gttgagaaag ttcccaagat
     3001 gttatttggg ttttcagttt tcaagtagaa tggacggtat gttgccacaa ctaaggcttg
     3061 gccaaagata gcccccaaga cttgcgcgat aatgtaaggt accacttgtg cccaagggaa
     3121 aagaccgcta actgcaagcc ctagagtgaa agcagggttg atgtgattcc cagatacgtt
     3181 accaaacatc aaggcaggga tcataacccc cataccataa ccaacagcga tgacgatcca
     3241 gccactttgg tgacctttcg tacctttaag ttcaacgttg gcaactgcac cattcccaag
     3301 aatgatcaaa atggcagttc ccaaaaattc agtggcatac ttcagtgccc atgtgaaatc
     3361 catttgctag attctcctta aaatttttta gatattcctt attttatcaa tttttaccct
     3421 atttagcaac tgatttctgg aaatattttt aaaaaaatag gattcatagt ttcccagttc
     3481 aacgtacttg taagcgtttt tattcttcac gaaagacctc attctcaaaa ccaggaaaaa
     3541 gcccacatct gtgatctttt ccttgttcct agattctaaa atctattatt tttcatcttc
     3601 ttttttcttg aagaatccta atcctagacc agctaacaag gtcatgatac cagcactagc
     3661 caaggtagca ttagcttctg tacctgtgtt tggcaattcc tctctcttac ctgtctcata
     3721 agtcggaact tctgggtctg gattcactgg agtttcagtt tttggagtac ctggttctgg
     3781 agttggctct ggtattggag tctcttgatt tggtagttct gggtttggtg tcggaacact
     3841 tggatgctct ggatttggtt gtgggattgg tttttctgga gtcggttcac ctggagtacc
     3901 tggttcctga ggacttggaa tttctggttt ttctggagtt ggtttatctg gtgttgataa
     3961 acggtcatac cttaccgtta tttctttatc actagagtct gacgtaactt cttgtgattc
     4021 aactgttgga atatctggat ctttgtactt gtcaatctta ccagatacaa cctcgtccca
     4081 gtttcctgtt gtccattcac cgtaggttac aactcccgtg accttgttct cagtttttgt
     4141 acggcttaag gttacaggtt gaacaacatc ttcttttaca ttttggttcg taactttatc
     4201 aacgtaatga atgatacgcg ttatagtctt cgtctcagta gaggttgctg ttttgggaac
     4261 cacttttcct caacattctc acggtaataa tagtcaactg ttgcaccgtc ttctggtacg
     4321 catttgcagg agttgctacc aaggtgtatg ttgttttcct tgtgataact cggtcttctt
     4381 tgtcctcagt tgttgttttc ccttcaatag tttttgattc tgtggtatac tcagaaccta
     4441 tcgctaaatc agcttttata acagactctg ccaacttctc ttggctacct tctttatagt
     4501 aattggctgt tactgtagca gtggttggcg cttcgctttc tctataaact aaggtcactg
     4561 ttctaccttt gcttacaata ttcccagtta aacttgcaga atttgtatct acttctttaa
     4621 aagtataata ttttccgtca gtagtagtca tgctactgag ttttttatct gtgacattat
     4681 agctggtacc aatcagttgt tttttattgg taatgtaggt tccgtcactt tctttttctc
     4741 caattccagt atcatttttc aatgatagca aacgcccttg ttcatcaaca tagcgaactt
     4801 tcacattttc tgagattagt tctgccaatt ctgaggtttt tttctttttc ttgatttctt
     4861 cggttatttt ccctttctct tcttcgggaa tatttagttt tggaatgatt ttttcaacaa
     4921 cggttcgtga tggttccaca gtatcttgga tgactgaaaa gtcagctaga attgggagat
     4981 tatactgaac acggtgactt tgagtgttta ctcctactct ttcattattc tctgaaaata
     5041 cttgtacggt ataagaaaca acatcttttc ctaatagaac atccccagta gagaaatagc
     5101 cgccttttcc tagtttgcta tctccagagc ccacttcttt cctaatctta tcagatagtt
     5161 ttttaccagt cagtacattc gttcgcacaa tccctttgtc tacccctaca aagtgggaga
     5221 attttttgaa ctcttcagaa ccagatctag cccaaccatt attaagggca tttgcttttg
     5281 tatttgtatt ctctctcaaa ggtttggcga ttagaattat attcatcggc gcttagagtt
     5341 gctgctatat ctgactcttg aataccaact tccttactac catttctagc ggcagtatat
     5401 gtgtatttat ctgtttctac ttctagctga ttatccttaa catgataggt aattttctcc
     5461 tggtataaga gtcttcagta gtttatatgc tctgtcatca tctttagctt tagaagcagc
     5521 tgagtttagc caagttgggg tattaggagt atctttatca gcctgtttca agacattatt
     5581 caagtcattt gctagcctat tggttatcct cactgtgaaa gttttcttcc agtctgaacc
     5641 acttttatta gacttagata tgctcatgaa atcctggtca acattcaaca caatatcgcc
     5701 ttatttttta gcatcttccg ttgcttctgc ttcaaattga tcatggagtt tttgttgctc
     5761 tggtgtatag tttttttatt tacacgattt aatgtactat tttttatttt agtattaaga
     5821 actccattaa tacgattttt aaactctgct tcaaaaaatc ccttaatttg ttggtgcgca
     5881 agttcaacat tcaaaccttt cttatacaag tcttcattct gaacgatttc ggctacacgg
     5941 tgaatggttt gatcgtatac cgtcttctct taaccagcag tatccttagt tttaatgact
     6001 actcgatagg ttcctttaga accgttatag tcatctagtt ttaaaaaagc attccaaaca
     6061 ttcaaaaata gttgaaactt cccttaatac atccgtcact ttctttttca agatagccca
     6121 agcttgctca ataggattca aatctggtga ataaggtggt agaggtaaga aaaagtgttt
     6181 atcttggatg cagagttcat ccaaaatgtt cttgggatga aaactagcat tgcccatgac
     6241 aataacatga ggtgtcttca aagcaggtag gagttgcgtt ttgaaccact ccacaaagaa
     6301 atcgcttgtc atgcttttct tgtaaatcat gggagctata aactctccgt ctacttgtcc
     6361 tgcaacaatt gaagttcgct caaaacgacg tccgctaatc ttttcataga ctttctcccc
     6421 tctaggagcc cgtgcataag gacgatagag atagcggtcg attcctgttt catcaatata
     6481 aacaggtgct aggtgcttta aactattaaa attcttaaga aataaggcta cttttctggg
     6541 tcttgttcct taaagatcgt catctttttt taaagtgacc ttaatctgct ttaaagctgc
     6601 ccatactgaa ggaacagcac aatcaaaatg tgccgcaatt tcccgtaaaa aagcatctgg
     6661 atgagcctct acaaaggctt tcaattcttc taaagggatc ttacgctttt tgacgactcg
     6721 ctttttccgc tctaagttcc tttgttcacg tctttctttt cccacgtgaa gagagttctg
     6781 acgccaacac caaaaaactt tggccgcctc gacatgtctg tgccctcctt tgatggaatc
     6841 taatgctcct tgtttaaaat cagtattata tgccatagct ttattagacc atgcttattt
     6901 taaactaagc aactatatca atgttttgtt atgagaagac ttactttttt tactgaaatt
     6961 gaatttttcc tagcctcaac atcatataac cccaaaattc aatctgacct ggtttgaata
     7021 gatgttcgaa gcagataagg ttttactaaa actaaaaaaa atgatataat caaattgatt
     7081 aaatgtttta aaaataaagg agaatttgaa tgtcttacca agaaaattac cagaaatggg
     7141 ttgattttgt ggagcttcct gactaccttc gtcaagattt ggaaaatatg gacgaaaaaa
     7201 ctaaggaaga tgccttctat acaaatcttg aatttggtac tgcaggtatg cgtggcttgg
     7261 ttggtgctgg tacaaaccgt atcaacatct acgttgttcg ccaagctact gaaggattgg
     7321 ctcgtttgat tgagtcaaaa ggtggaaacg agaaagaacg cggtgtagca attgcctacg
     7381 atagccgtca cttctcacct gagtttgcct ttgaatctgc ggcagttctt gctaaacacg
     7441 gcatcaaatc ttacgtattt gaaagccttc gtccgactcc agaactatca tttgcagttc
     7501 gtcatctcaa ctgtttcgca ggtatcatgg tcacagccag ccacaaccct gctccattta
     7561 acggttacaa ggtttacggt gaagacggtg gacaaatgcc tccacacgat gcggacgctt
     7621 tgactactta tatccgtgca atcgaaaacc catttgcagt tgaagttgct gatgtggaaa
     7681 ctgaaaaagc ttctggcttg attgaagtta tcggcgaagc tgttgacgta gaatacctta
     7741 aagaggttaa ggacgtaaac atcaacccag ccttgattga agaatttggt aaagacatga
     7801 agattgtcta cacaccactt catggtactg gtgaaatgtt ggctcgtcgt gctcttgccc
     7861 aagcaggatt tgactctgtt caagttgttg aagcgcaagc aactgctgac ccagacttct
     7921 caactgtaac atctccaaac ccagaaagcc aagcagcctt tgcccttgct gaagaacttg
     7981 gtcgtcaagt tggtgcagat gttcttgtcg caactgaccc agacgctgac cgtgttggtg
     8041 ttgaagttct tcaaaaagat ggtagctacc tcaacctttc aggtaaccaa atcggtgcta
     8101 tcatggctaa atacatcttg gaagctcaca aaaacgctgg aactcttcct gaaaatgccg
     8161 ctctctgcaa atctatcgtt tcaactgact tggtaacgaa gattgctgaa agctacggcg
     8221 caactatgtt caacgtcttg acaggtttca aatttatcgc tgaaaaaatc caagaattcg
     8281 aagaaaaaca caaccacact tacatgatgg gatttgaaga aagcttcggt tacttgatta
     8341 aaccatttgt acgtgataaa gatgccatcc aagccgttct tgtcgttgct gaacttgctg
     8401 cctactaccg ttctcgtggt ttgacacttg ctgacggtat cgaagaaatc tataaagaat
     8461 atggctacta cgcagaaaaa acaatctctg ttactctttc aggtgtcgat ggtgctgaac
     8521 aaatcaaagc gattatggct aaattccgca acaatgctcc aaaagaatgg aacgcaacag
     8581 ctatcactgt cgtagaagac ttcaaggcac aaactgctac tgttgctgac ggtactgtta
     8641 caaacttgac aactcctcca agtgatgtgt tgaaatacac acttgctgac ggttcatgga
     8701 ttgccgttcg cccttcaggt acagaaccaa aaatcaagtt ctacattgca gttgtagggg
     8761 aaaccaacga agaatcgcaa gctaagattg ctaacatcga agcagaaatc aatgcatttg
     8821 taaaataaag acctataaaa gatgagacca accaacctca tcttttttcg tatcaaatct
     8881 aagcaatttt agaaacttta tggcatacta gacttatcaa tgaaagcgag gtaatctcat
     8941 ggaacaattt ttagataaca tcaaagacct tgaagtcact acagttgtgc gtgcacaaga
     9001 agctcttgat aaaaaagaaa ctgcaacctt ctttatcggt cgcaaaactt gcccttactg
     9061 ccgtaaattt gcaggtacat tgtcaggtgt cgtagctgaa accaaagctc acatttactt
     9121 catcaatagt gaagaaccaa gccaactcaa tgatttgcaa gcattccgct cacgctatgg
     9181 aatcccaact gtaccaggtt ttgttcatat tacagatgga caaatcaacg tccgttgcga
     9241 ctcttcaatg tcagcacaag aaatcaaaga tttcgcagga ttgtaaaaaa tcagctaaca
     9301 agtcttaaaa tcacaaaaac gcataatgta aggtgcaaaa accttgatat tatgcgtttt
     9361 attgttccta aaacagttac atcagcattt cattacagaa cattttaatt ttggaagtca
     9421 aatcaatttt taacaatgtt ttagaaatca cagtgggcta ttccatattc aattatccag
     9481 attcaatcgc tattatcgtt ttcctagatt acaaaaaaaa ctgcgttttg ctaatcaaaa
     9541 cgcagtttca gccactattt tatcttactg tccttctttt acttctttgg ttgctacatc
     9601 ttctccaaac catttttggc tgatttcttg gaacttgccg tccttgtaaa gactagaaaa
     9661 agcttcattt atcttcttaa ccaaggttgt atcttcctta cgggctccaa ccgcaaaagc
     9721 ttctgtttct agtccaactg taaagacatt ataatcgttt aaaacacctt ctgcttctaa
     9781 ataatagttt gcatagacac ggtcaatcaa tagaccatca attcgatcgt ttttcaaatc
     9841 aatcaaggct tcattaaagg tttggtattg attcgcttcc ttattagcga caatattctt
     9901 caaaatttct ggatttgctt caaagtccgc ataaccagat gaaccagctt gagctcctaa
     9961 tgtctttcca gtcatatcct ttgcagtcgt gataccagat gatttcttcg taaccaatac
    10021 ctgctcattc ttcatatatg agttactgaa agccaccttt tcacggcgtt cgtctgtagc
    10081 ggaatagcca ttccaaatca gatcaatcgt tccttttgtc aattcagctt ctttcaaatc
    10141 ccaatcaatc ggttgccaat ttaccgtgat tccgtatttt tcaaaaacag ctgtagctaa
    10201 atcaatatca aatcctgcat aagaaccatc tttctgagca aatcccattg gaacaaaagt
    10261 actatcaaat ccaatagtaa tagacttgtt agactggtac tttgaccaat tatctccact
    10321 agtttcgcta gaatttttcc cacaagctac taagaacaaa gcagtcatca gactgactaa
    10381 tacaagcatc cattttttca tccatttttc ctcctattta ggttctactt tcaataatac
    10441 atctgcgata ttttcagcaa actgcaaatc atgggtaacc acaatctggg tcatcccaag
    10501 ttccctattt tgcaagatta gcttctccac ttccaaacgc aattctggat ccagggcaga
    10561 agttggttca tcgtagccaa tgatttctgg gtcaatcatc atagcacgcg ccaaagccac
    10621 ccgctgcttt tgcccaccag atagtgagaa aggataggcc tctgcgtgtc ctcctagtcc
    10681 taactgttcc aagagtccac tcgccttctt ctcagcctct tcctgcttca ttcccatggt
    10741 cttcacaggc gataaagtca aattttccag aactgataga tgaggaaata gttgaaaatc
    10801 ttggaagaca aatcccagta ggttgcgctt ctgcaattca tccagctcta aaggttgtcc
    10861 attataaaag atttgccctg aatcaatggt ttcaagaccc gcaagcatac gtaaaagagt
    10921 cgtcttacct ccaccagaag gtccaacgat agccaggatt tgcttttcag gaatacttag
    10981 actgaaatta gacaggattt gtttgtctcc aaagacttta ttgatatttc gtaattctaa
    11041 catggcagcc tcctatctat aataactata cttcttctca acttttttgg aaataattgt
    11101 cacaatccca atcaaaatca aataaatggc tcctgccaag aacataggaa ctagactagc
    11161 atcgcggtta gcagctgttc gactagccaa gataaggtct gaaattccga gagcatagac
    11221 caaagaagta tccttgacca aactcataac ttcattaaag acactaggaa gaacgatctt
    11281 ggtcacttgg ggcaagataa tatagcgcac tctgtcaaaa gggctaaact tcaagacctt
    11341 ggcagcctca tactgtcctc ttggaatagt gtcaattccc ccacggaaaa tttctgcaaa
    11401 gtaagctgca tagttgagaa caaaggcaat aatagctgca ggaaggcggt ctaaacgaat
    11461 cccaatactt gggagcacat aatagataaa aatcagttgc aagagtaagg gggttcctcg
    11521 cataacccag atgtaaatgt taatcagata atggaggggc ttccaatgga cttgcaaggc
    11581 aaaggcaatc aaaacgccca agggaatcga aaatagcaag accagtgcaa agacctgtac
    11641 agtcgtgctc gcaccattca gtaaactcgg taatatctca aataaataag acatactgca
    11701 cctcctaaaa gtaattgttc ctattatagc atgaataaac aagattacca agtatttttg
    11761 taaaaagatt ctaccatata caaataaaaa ccgtagctat caaaagagat aaactacagt
    11821 tctatatgtt taaacattct tacaatctct caaacagttc ttgcatttgc acatcatctg
    11881 gaaccagttt taagtaagtg tgagcatgga cttttgcttc ttcaaaatgt cccaattcac
    11941 gcaagagata gatatagtgt tccagaaatt ctggattgtc cttcaaatct cctgtcaact
    12001 cttgataatg ctcataagca gtatccaaat cgtccatttc ttgataagaa cgagcaatca
    12061 tccacttggt caaaagattt tctggctcct cactctgcaa gtctagaata tcctcataac
    12121 gctcctgctt cagataaata gtggctaaac gaagcaagat ttcttctgta tcctcagcgt
    12181 cttcttttgc agcaagaaga taattttctg caccactagc atcatgcaat tcataagaaa
    12241 attgtgaagc agctagcaag aggcgagttt caaagggatt tttctctaat ccttgcttag
    12301 cgatacgcag ggcttcttga acttgatgtt ccttatgtaa agcctgactg tacccatact
    12361 catagccttc aaagtcagga gaaatggtat caagctgctt aaagtagagg gtggcttttt
    12421 gatattcttc ttgatcaaaa taaagactgg ccaactcaaa agctgttaag tcatcgtatt
    12481 ctaactccag ggctttttct aaaaactcag tagccgtttc aaatttccct aactgagcat
    12541 aggcaaagcc aattcgttga taggtggaaa tgcccgtttg ctcataaatc gagcgattat
    12601 ctaactgggc ataagcttga atagccgctt ggtaattttc caactcacta tccaactctg
    12661 ccaaacccaa catcaagaga gaatcctctg agtaggtcaa ggcctccaat aatttctcac
    12721 gtgccacatc tgtcaaacct tccagctggt aaaggtctgc cttcagagcc aaagacgaga
    12781 cataccagtc actatcagct tggatttcct caagataggt aaaggcttct tctatttgac
    12841 catcctcgct agcaattgca gctagattaa gatgaacctc tggaaaatcc tctacaattt
    12901 tcaggtaaat ttccttggcc tgaggataga aaccaatccc ttcaagataa gttgccaact
    12961 catacagaag atcacttgaa tcattttcta aagctttggc gaaataatgc tcagccttag
    13021 ttaaatcttg ctcctccaaa gcctgtaaca tctgttgact attgttcact cttgacttcc
    13081 tcaccctcta attcatatag accactgact accttatacc attcgaaaat ggctgaaatg
    13141 acaaccttag cagaggcata aaccggaata ccaagtaaaa ctccccagat accaaacata
    13201 gatcctgaag ttaacaaaac aaagagaaca ttaatagggt ggatgtttaa ttgacttccc
    13261 aaaatcaatg gagagacaaa acggccttca atagtttgtt ctacaataaa gacaatcact
    13321 actttcaaaa gcatgactgg accagcaatc aaacccaata ctagggcagg aagcatggct
    13381 agaaagctac caagataagg gaccagattt aaaataccag cagtaacccc cagcgtaacc
    13441 gcatagcgta gaccaataat cttgaagaag atgataaaca ttactgctac aataatagcc
    13501 actgtcactt gccctcgaac atagttggac aactgttgat tcacatctga tagaacttgt
    13561 ccaacaggtt ccttcaattt tcttggaatg aattgggtca aatagttacg caagcctttc
    13621 ccatcacgca agagataaaa gagcatgaaa ggaacgataa tcaaggcaac aatcacttga
    13681 gaagctccgc taataaaggc actcacccag ttgactgcct gagatgaaac cttacttgcc
    13741 aaaactgtag cctggctaga aaaattggtc aaaacttgct ctaattgagg tctgaaatca
    13801 tctggcaggt gctgggctac caatccatta acaatcctat ctatatcttc taagtaaaca
    13861 ggaacgtttc ttgcaaaggt caaaacctga cgttgcagat ttggaatggc gactgccaag
    13921 ccccaaatga taaagagagc gatgataaca aagacaatag tgatagctat aacacgatta
    13981 accttatgct tctccatcca atcaacaata ggattcaaca aataatataa caaaccagac
    14041 aaaatgactg gcaacatcac aactgctaaa aagtctaaaa caggtgaaaa tagaaaacta
    14101 atcttactta aaataaaaag attcagtccc aataataagg ttactaaaaa taccgtaatt
    14161 gccttgttat ctaaaaacca cttgaaaaac caagataggc taaaatgttt ctctttttgc
    14221 tccataaata cttcctttct attgttctct cattatacca tttttaaaac aaaataactc
    14281 ttttttaaag tgcttacatc taattggata tctttaattt acttgttttt gtgatataat
    14341 agtcttatct ttacacagag gtatagtaat gaaagaaact gtttattttg gaacttatac
    14401 acgtcgtact tctcaaggga tttacaaggc agactttgat acagaaactg gtcagctttc
    14461 aaatctagaa ctttttgcag ctgagccaag tccaacctac cttgcctttg accagcacca
    14521 acatttatac actgttggta gccaagacga taagggggga attgcagcct atcaaactga
    14581 cgggactgtg ttaaatcatg ttgttgaaga aggagctccc cactgttatg ttgctgtcga
    14641 tgaaaagcgt gatttggttt acgcagctaa ctatcacaag ggacaagtcc ttgtttataa
    14701 acgccaggaa gatggtagtc ttctacttag tgatatggat caacacagtg gccaaggtcc
    14761 acatgaaaat caagcttccc cccatgttca ctatacagat ttaacacctg accactatct
    14821 agtgacctgc gacttgggaa ctgaccaagt catcacctat gacctcgatc aagaaggaaa
    14881 gttatctaag ctctatacct atcacagcaa gccaggagca ggctcacgcc atatcatttt
    14941 ccataaccac tataaaatcg cttatctcat ttgtgaactc aatagtacta tcgaagtttt
    15001 aatctacgat ggcgttggcg aatttgaacg tatgcaggtc atttcaactt taccggaagc
    15061 ttacgaaggc tttaatggaa ccgctgctat tcatctctct aaagacggta aatacctcta
    15121 cgcttctaac cgtggccatg attctatcgc agtatatacc atccttgcgg acggtagctt
    15181 agagttatta gaaatcgttc caacgcatgg tcagactcca cgtgattttg atttgacacc
    15241 cgaccaaaaa tttctcattg ttgtccatca agactctgac aatgcaactg tctttaaacg
    15301 taattgtgac aatggtcgtc tagcagaact ctccaacgac ttccatgttc ccgaagcagt
    15361 ctgcatccgt tttgctcctt aaaacaccat aaaaaatgaa cttggtattt tcaagttcat
    15421 tttttcttat agtctatttc cgacattaat acggttaata gcacgttgca aagcaatctt
    15481 agcacgacgt tcttggtcaa tcaaatgttt gtcttgtgct tcttcaattg cacgttctgc
    15541 acgaagtttg gcacgttctg cacgactgat atcgatatca cgagcacgtt ctgcagagtc
    15601 agcgacgatt gtgatcatat cattggcaat ttcaataacg ccaccgttta ctgcaatcca
    15661 gttcacgtga tctttatcat cgatacgttt tacctttact tcatcaactg ctaaaaccgc
    15721 aatcatattt tcatgtcgtg gcaagatccc catctcacca tccagagttc gaaccgatac
    15781 atagctggca tggtgatcat agacgagacc atctggtgtc acgatctgga cagttaactg
    15841 agccatagat cacctcttaa aatcccattt tttcagcttt tgcaatcaca tcttcgatag
    15901 aacctacacc acggaaggca tcttctggca agtggtcgta tttaccatca aggatttcct
    15961 taaagccacg tacagtttca gcaactggaa cataagaacc tggctgacca gtaaattgtt
    16021 ccgcaacgtt gaagttttgt gacaagaaga actggatacg acgggcgcga gcaaccaagg
    16081 tcttttcttc atcagaaagt tcatccatac caaggatagc aatgatatct tgcaattcat
    16141 ggtaacgttg aaggacacgt tttacttcag cagcaactgc atagtgctct tctccaacga
    16201 tttcaggtgc caaggcacgt gagcttgaag caagtgggtc aacggctggg tagataccca
    16261 attgtaccaa cttacgttcc aagtttgttg ttgaatccaa gtgagcgaag gctgttgctg
    16321 gcgctgggtc agtatagtca tccgctggca catagatagc ctggatagag gttacagaac
    16381 ccttcttggt tgatgtgata cgttcttgca attgacccat ttccgtagca agtgttggtt
    16441 ggtaaccaac ggctgatggc atacgaccca aaagggcaga tacttctgaa ccagcctgag
    16501 tgaaacggaa gatattatcg ataaagagaa gcacgtcttg gccttccaca tcacggaagt
    16561 attcagcgat tgtcaaacca gtaagggcaa cacgcatacg tgctcctggt ggctcattca
    16621 tctgaccaaa gaccatggct gttttctcga taacgcctga ttctttcatt tcccagtaaa
    16681 ggtcattccc ctcacgagta cgttccccaa caccagcaaa tactgaaata ccaccgtgct
    16741 cttgggcaat gttgtgaatc aattcttgga ttaagacagt tttaccaact ccggcaccac
    16801 cgaaaagtcc aactttacca cctttaaggt aaggggcaag aaggtcaata accttgatcc
    16861 ctgtttcaag gatttcagaa gaggtagaca actcatcaaa agttggagct tttttatgaa
    16921 ttggctgacg ctctgcgtct tctgtaaaag gagcttccaa gtcaatggta tctcccaaaa
    16981 cgttgaagac acgtcccaaa gtttctttac ctactggtac agagattgga cgacctgtgt
    17041 ccaatacttc cattccacga gtcaacccat ctgttgattc catggcgata gtacgaacca
    17101 taccatctcc taactccaag gctacttcaa ggacgatttt tgtttttctt tcgtcatttt
    17161 tgtagacgac aagtgcattg ttaatctcag gaagtttttc ccctgctgca aacaaaacgt
    17221 ctacaacggg accgataacc tgagcaattt tacctgaact catctccttc tcctattcta
    17281 tataagatac tgtcggttcc tagctcaact aggtcctaag ttcattttca tacgagctgg
    17341 actagagcct attctaaggc actagcacct gctacgattt ctgtaatttc ttgtgtaatc
    17401 gccgcctgtc tggcacggtt atactgaatt gttaaatcat tgatgacttt cttagcatta
    17461 tctgtcgctg tttgcatggc tgtcataccc gcagcattct cagctgtctt ggcatcgata
    17521 atggcaccgt aaatcatact ttctgcaaac tgaggcaaca actgctccag aatttcttct
    17581 cggctggttt ccaattcaaa agtcaagctg tactcttcat ccgcttcatt tggatccaag
    17641 tcaacaatcg gaagcatttg ttccacacgc atttgactgg ttagcgtatt gacatggtgg
    17701 ttgtagcaaa cataaagctc atcaaagagt tcattttggt acatttcaac agtttttgaa
    17761 ataatcttac gaacttgatc aaagctaggt tggtctgcca agccacgtaa ttcataaagt
    17821 ggttgaatac cgcgagcctt aaagaaatca gctcccatcc caccgataca gatcatttca
    17881 aaacctttac cgtctgggtg gtattcttct ttcaactcca taacagcttt caaaatagag
    17941 gaattataac ctccaaccaa accgcggtct gaagtgataa cgatatagcc tgtcttcttc
    18001 acagaacggc taatcaacat cggattagtt gaagcaccag ctccattacc atgaaggata
    18061 tctgtcaaaa gtttacgcac tttctgagcg taaacttgga agttgcgagc agcttcttca
    18121 gagcgaccta acttagcagc cgataccatt tgcatggcat tagtgatttg actcgtattt
    18181 tttgttgagg cgatttttgt tttaatatca tttagagaca ctgccatctg acacctctat
    18241 tcttattgga agctagattg attgagaaac tctgtaatcg cagcatccaa gactgcttct
    18301 tctggcaagt cttttgtatc acgaatggtt tccaaaatct ctggatgttg agcatcaaag
    18361 aaggcatgga actcttcctc gaaacgaaca atatcatcta ctggaacagt atccaagaaa
    18421 ccatgtgtca aagcataaag aatggttact tgtttctcaa caggtaatgg tttgtgaaca
    18481 ggttgtttca agacctcaac ggtacgacgt ccacggttca acttagcctg tgttgctgcg
    18541 tccaagtcag aaccaaactt agtaaaggct tccaactcac ggtatgaagc aaggtcgata
    18601 cgaagtgtac cagcaacctt cttcatggct ttgatttgtg cagaaccacc tacacgagat
    18661 acagatgaac ccgcatcgat ggctggacga atacctgcat tgaagaggcc atcgccaagg
    18721 aagatttgtc catcagtgat agaaatcacg ttggttgcga tataggctga gatatctcct
    18781 gcttgtgtct cgataaatgg tagggctgta attgatccac caccaagttc atcagaaact
    18841 ttagctgagc gctcaagcaa acggctgtgg agatagaaaa catcccctgg gaaggcttca
    18901 cgacctggag gacgacgaag caagagcgac agttcacgat aagctaccgc ttgttttgat
    18961 agatcatcgt atacaatcaa aacatgctta ccttgataca taaattcttc cgccatagcg
    19021 accccagtat aaggagctag gaagagcaat ggagatggtt gtgaagcaga ggctgtcaca
    19081 acgattgtgt agtccaaggc accgtactga cgaagtgttt ctacttgcgt acgaactgtt
    19141 gattcttttt gtccaatcgc gacgtagata cagatcatat cttgatcttt ttggttcaag
    19201 attgtatcaa tcgcaatggt tgttttccct gtctgacggt caccgataat caactcacgt
    19261 tgaccacgac caatcggtac aagggcgtca atagctttca aaccagtttg caatggttct
    19321 gaaacagact tacgttgcat aacaccagga gctggtgctt ctactggacg agttttatca
    19381 gtgtggattt ctccaagacc gtcaactgga cgaccaagcg gatccacaac acgaccaatc
    19441 agactttcac ctacagggac ttccatgatt ttccctgtac ggcggattgt atcgccttca
    19501 cggatatctg taaagtcacc taggatgata ataccaacgt ctgttgactc caagttttga
    19561 gccataccat aagagccgtt ttcaaaattc aacaactctc cactcatgac attttcaagg
    19621 ccgtgagcac gcgcgatacc gtccccgata taggttacaa cacctgtttc agtcacatca
    19681 aaattgggtt tgaaattttc aatttgttgc ttaattaaag cgctgatttc ttgtgcgtta
    19741 attgccaaaa gaacaccact ttctatttca aattttcttt aacaacttta agttgttgtt
    19801 taatactcac atcaattgtc ttgtgattgg caaaaatgac aaaaccacca atgagacttt
    19861 catcgatttg ttcttttaca ctccttactt tcagagacat ttttttctca atcaaaggga
    19921 gcaaacgagt cttctgttca tcagttagag gatgagcaga cgtaatcgtc acttcaaatc
    19981 gatttgtttc tttttcaagt cggttcaagc aatctacaag cacatcataa aaaagatttg
    20041 ctctgtgatt gtaggccaga acctggataa agttttgtaa taaaggcgac acagaatctt
    20101 ggaaaaaagc aattgttttt tccttatccg actcgtctac tgccacttgt tttaaaaaag
    20161 aaggcagacc tgttttttca acaacttgct tgatttgagt caagtctgaa aagatacggt
    20221 cttcttctcc tttttcaagt accaattgga caaaaggcat gctgtatttt tcaattacct
    20281 ttactgtttt cttgtccatt aagcttctcc tagctgatcg atatactgat caatgagtgc
    20341 tttatgggca tgactgtcaa ggttttgtga gatgatttta ccagctaagc tgatggtcaa
    20401 atctgcgacc tcacccttaa cactctgtaa agcttctact ttattttgag caatttcttg
    20461 attggctttt tcttttaagt gtcctgcttc tagtttagca tctgctaaga tattagcctt
    20521 actttgctca gctgtttcct ttgcattttc aatgattgtc ttagcttctt tacggctacc
    20581 agccaattca tcttcgcgtt tttgagccaa tacttctgct ttttgacggg cttcttcagc
    20641 tctgtcaata tctgaagcaa ttttttcagc tctttcttcg aaaatgcctg taatattaga
    20701 ccatgcaaat tttttaatca agactagcaa aagaataaaa gagccagtga ttaaaataaa
    20761 attaccaatt aattcaccta ctgttacgtg catcagttac tcctttctat tcctcttcaa
    20821 tattaatctt attccctaaa tacacagatg tcaaaagagt aaaaacataa gcttggatgc
    20881 aggaaataaa gacagaaaat gcagtccaag ccaaattagc tccaaaggct actggatacc
    20941 aataaatagc ttggtgggaa agaagaagta acaaactcgt catgacctct cctgcaaaga
    21001 tattcccaaa aatccgcaaa gccaaagata agaagtttgt aaattcttcc aagatattca
    21061 tcggtatgac aaaaacagga ctcataaaag attttatact ttttttaaat ccacgacgac
    21121 gaacgctttc tatatgtgtc aacaaaatga caagaaaaga taaggttaag tcatactgta
    21181 aattagcggt tggcgaactc caccagttag tcccatcgat cgtttgaagc tttgtcatta
    21241 agccaaggtt attggcaatc accatgaaaa ggaataaaca aaggaaaaag agtgagtaat
    21301 ctttcatgta gcgcgaacca atgttaggtt ctgtaaatcc aataacaaag tcatagacat
    21361 actcaagtac attttgcttt cctttgggtt tcaaggtcat attgcggctt gcccaataaa
    21421 taaagacaaa aataactccc acaatcaaca aagtcatggc taacatagtc agattgaaga
    21481 taacaggacc aatagagatg attggattaa tactttcttc catctaaaat cctccctttt
    21541 cttctcgttc catgtattta tttgataata aatgagaaga caagagttac aaagaaagtt
    21601 ccttcaataa aggcaacacc taaaaacatc aaactacgaa actcagaaag catatctggt
    21661 tggcgtgcta ctgatttaaa cagtccattc atcaataaac cttcaccgac agatacgccc
    21721 atacaggcaa tacataagcc taaaaatgtt aaattcataa caaattctcc tttaaaataa
    21781 aatttactta cttagtgtag tcctaaaatt taaatttgtc aacttttaac cgctattgga
    21841 agcgtttaat aaaaattatt tccattttgc taaatttcaa gttcaagtta gccatccaaa
    21901 agtcttctct gggtgacttg tagtccaagc attttttagg atagttgtta atccactttt
    21961 cgatgaatgc gacttctttg ggagtcattt tcttggttcc cttaggtaac catctacgaa
    22021 tgagcctgtt gtgattctca ttagttcccc tttcccaaga ggcataggga tgtgcataat
    22081 aaatgtgctc ctcagaaaat acattagaca agcgattgaa ttccgttcca ttatctgccg
    22141 tgatggaaag aatcttgtgt tgttttaaga tgagttttag agcctgattg accacctcag
    22201 cacttttatt tggaatcaat cggatgatct gatgtctact ttttcgatcc gtcaagacaa
    22261 gcaagcagta gtttttcgct ctcgtaagta gaactgtatc aatctcataa tgcccattct
    22321 ccaagcgaaa attgatagct tcaagccgct gttcgatgga ttgaccagca ggtttaaagt
    22381 tggtgctggc ctgtttctta agcgcttttc cttttctagg gtaaagcaga tcctgcttgc
    22441 tttaccccaa ttttccatga tgaatccaat agtaaatggt tgaaattccc acgttaaccc
    22501 ctttagccat aaccatcatt tcaggcaaaa atttttggtt atgatagtgg agaatctttt
    22561 cctttagttc cttggtcacg cttgatctct tgaccgagtg cttgcgatta ttttcataag
    22621 actgttgagc atagtcggca gaataaacct ctttgaagcg cccttttcca agacattttc
    22681 ggactgtcct acgcttgatt tcagtgtgga tagtttgagg agcttttcca agtagagagg
    22741 caatttctct atttgatttc cctttgttcg ccttttgtag tataatggtt ttgcatctct
    22801 gtgcctttct tgtgtttgtg gttgaacaac aagtataaca caaaggtgtt ttcttatgcc
    22861 tacaagagct ttcattattt ccattttctt ttggatttca ctctattctg aaaaacttgt
    22921 gtatatttac tgaagctagc aagtcttacc tgtaaattta atgaaagcaa cacaaaatcc
    22981 gagaggggaa tctcggatta atgtcaattt aaaaagatag agagttttta gtttaaataa
    23041 attgttttaa aatatcaaca acatcacttc ttttcttaac ctgataagcc ttgattccta
    23101 attttggggc tacgattata ttgtcctcaa tatcgtctag aaagacacaa tttctaggtt
    23161 ataactggta tttatcgata gttactcata tatatcagtc cacctccata ctcaccttac
    23221 agattctttt gataataata tctttgccta atgtagagat agtcttgcaa agaaaaaact
    23281 tccttgtagc catgtttctg ataaaagccc ggtgcctgga actggtaagt attgacaaag
    23341 gcaaaacaac aatttcgatt tttagcttca ctttctgcct gttgcaatag ttttgaaccg
    23401 attccttgcc ctcgcagttc ctcttttaca aacaaatact cgatttctag ccaatttcca
    23461 aaagtctctg ctatcaaacc cgcaaggaga ttgccctttt catcttcgac ataaagatta
    23521 agtggctcac tttcagcctc ttctcttttt gaacggttat aaacacgaat cagattccct
    23581 atttcttgcg atttatatga ttccttattt tccaatctaa agtataatga aatgaaataa
    23641 aacatgcgca aatcgattaa ggaatttaat ctaatttcta acaatgtctt agaaatcaaa
    23701 gtgtactatt ttaacttcaa tgcactatac atctaatact caatgaaaat caaagaacag
    23761 actaggaaac tagccgcagg ttgctcaaaa cactgttttg aggttgtaga tagaactgac
    23821 gaagtcagct caaaacat
//
LOCUS       DAVKWZ010000030        23299 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000030 DAVKWZ010000000
VERSION     DAVKWZ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23299
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.030"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            584..2737
                     /locus_tag="VW854_001230"
     CDS             584..2737
                     /locus_tag="VW854_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEV0262465.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPYKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTGINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLIALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            2750..4099
                     /locus_tag="VW854_001231"
     CDS             2750..4099
                     /locus_tag="VW854_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEV0262466.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAMETGASLASQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4269..4976
                     /locus_tag="VW854_001232"
     CDS             4269..4976
                     /locus_tag="VW854_001232"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEV0262467.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIAGDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQELK"
     gene            5178..8903
                     /locus_tag="VW854_001233"
     CDS             5178..8903
                     /locus_tag="VW854_001233"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEV0262468.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLAEDLFAPAEKHIFSEQGTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDAFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTVFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            8996..10438
                     /locus_tag="VW854_001234"
     CDS             8996..10438
                     /locus_tag="VW854_001234"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEV0262469.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10475..11497
                     /locus_tag="VW854_001235"
     CDS             10475..11497
                     /locus_tag="VW854_001235"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEV0262470.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11494..12039
                     /gene="purN"
                     /locus_tag="VW854_001236"
     CDS             11494..12039
                     /gene="purN"
                     /locus_tag="VW854_001236"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEV0262471.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKTDYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            12123..12632
                     /locus_tag="VW854_001237"
     CDS             12123..12632
                     /locus_tag="VW854_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV0262472.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            12657..14204
                     /gene="purH"
                     /locus_tag="VW854_001238"
     CDS             12657..14204
                     /gene="purH"
                     /locus_tag="VW854_001238"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEV0262473.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLNGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            14326..15588
                     /gene="purD"
                     /locus_tag="VW854_001239"
     CDS             14326..15588
                     /gene="purD"
                     /locus_tag="VW854_001239"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEV0262474.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVPTATYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGVIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTIADTVKEA
                     QASIYQELYQQKIEGLFYRTDIGSKAIK"
     gene            15991..16479
                     /gene="purE"
                     /locus_tag="VW854_001240"
     CDS             15991..16479
                     /gene="purE"
                     /locus_tag="VW854_001240"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEV0262475.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            16466..17557
                     /gene="purK"
                     /locus_tag="VW854_001241"
     CDS             16466..17557
                     /gene="purK"
                     /locus_tag="VW854_001241"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEV0262476.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPVSRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSQDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLADKAKAMAVRIAEQLNLSGTLCVEMFATADDIIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEKYVTENPSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVEEFGEGIDF"
     gene            17567..17794
                     /locus_tag="VW854_001242"
     CDS             17567..17794
                     /locus_tag="VW854_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HEV0262477.1"
                     /translation="MIQIIVNTFIEKYKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIEKEEFE"
     gene            17857..19155
                     /gene="purB"
                     /locus_tag="VW854_001243"
     CDS             17857..19155
                     /gene="purB"
                     /locus_tag="VW854_001243"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEV0262478.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCNKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(19210..23148)
                     /gene="strH"
                     /locus_tag="VW854_001244"
     CDS             complement(19210..23148)
                     /gene="strH"
                     /locus_tag="VW854_001244"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV0262479.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAVLQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPATEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSHLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVAAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLDQLKRIVDKASELGYSDVHLLLGNDGLRFLLNDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYVIGNHKQDEAY
                     PLSKAVENSGKVPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGSPVTHKGDESG
                     LAPTTEVKPRLDIQEEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPA
                     EESKALPQDPAPVVTEKKLPETGTHDSAELVVAGLMSTLAAYGLTKRKED"
BASE COUNT         6328 a         4655 c         5560 g         6756 t
ORIGIN      
        1 tctaaaaata taaccttaag ttttagcatt ttactttgtt atttatttaa tccatcggat
       61 ataaagtttg cctatttcag cgtattgaca gtattactaa ttgggataac aaattatgcg
      121 agatttaaaa atttaagatt gacagtagtg ttttctgtac catttttatt taatgctata
      181 atgtctatta taaatagttt ttagaaatac taaaattttt ttctttactt aataatgata
      241 tgaaatcatt tcggttttgt taacttgtaa ttcttatatt ttctaggtag aacgtttgtt
      301 ctgcctattt ttttatccaa aaagtgcagt tgggagggag ataggctcat ttgggaagga
      361 agtccagttt ttgtttagtg attggggtaa gatagttgtt atcagatgag tttatactct
      421 tcgaaaatca aattcaaacc acgtcaacgt cgccttgccg tatatatgtg actgacttcg
      481 tcagtcctat ctacaacctc aaaacagtgt tttgagcagc ctgcggctag tttcctagtt
      541 tgctctttga ttttcattga gtattaggga aaaggagatg aatatgaaat ttgggaaacg
      601 tcactatcgt ccgcaagtgg atcagatgga ctgcggtgta gcttcattag ccatggtttt
      661 tggctactat ggtagttatt attttttggc tcacttgcga gaattggcta agacgaccat
      721 ggatgggacg acggctttgg gcttggtcaa ggtggcagag gagattggtt ttgagacgcg
      781 agccattaag gcggatatga cgctttttga cttgccggat ttgacttttc cttttgttgc
      841 ccatgtgctt aaggaaggga aattgctcca ctactatgtg gtgactgggc aggataagga
      901 tagcattcat attgccgatc cagatcccgg ggtgaagttg actaaactgc cacgtgagcg
      961 ttttgaggaa gaatggacag gagtgactct ttttatggca cctagtccag actataagcc
     1021 ttataaggaa caaaaaaatg gtctgctctc ttttatccct atattagtga agcagcgtgg
     1081 cttgattgcc aatatcgttt tggcaacact cttggtaacc gggattaaca ttgtgggttc
     1141 ttattatctg cagtctatca ttgataccta tgtgccagat cagatgcgtt cgacactagg
     1201 gattatttct attgggctag tcatcgtcta catcctccag caaatcttgt cttacgctca
     1261 ggagtatctc ttgcttgttt tggggcaacg cttgtcgatt gacgtgattt tgtcctatat
     1321 caagcatgtt tttcacctcc ctatgtcctt ctttgcgaca cgcaggacag gggagatcgt
     1381 gtctcgtttt acagatgcta acagtatcat cgatgcgctg gcttcgacca tcctttcgat
     1441 tttcctagat gtgtcaacgg ttgtcattat ttcccttgtt ctattttcac aaaataccaa
     1501 tctctttttc atgactttat tggcgcttcc tatctacaca gtgattatct ttgcctttat
     1561 gaagccgttt gaaaagatga atcgggatac catggaagcc aatgcggttc tgtcttcttc
     1621 tatcattgag gacatcaacg gtattgagac tatcaagtcc ttgaccagtg aaagtcagcg
     1681 ttaccaaaaa attgacaagg aatttgtgga ttatctgaag aaatccttta cctatagtcg
     1741 agcagagagt cagcaaaagg ctctgaaaaa ggttgcccat ctcttgctta atgtcggcat
     1801 tctctggatg ggggctgttc tggtcatgga tggcaagatg agtttggggc agttgattac
     1861 ctataatacc ttgctggttt actttaccaa tcctttggaa aatatcatca atctgcaaac
     1921 caagcttcag acagcgcagg ttgccaataa ccgtctaaat gaagtgtatc tagtagcttc
     1981 tgagtttgag gagaagaaaa cagttgagga tttgagcttg atgaagggag atatgacctt
     2041 caagcaggtt cattacaagt atggctatgg tcgagacgtc ttgtcggata tcaatttaac
     2101 cgttccccaa gggtctaagg tggcttttgt ggggatttca gggtcaggta agacgacttt
     2161 ggccaagatg atggttaatt tttacgaccc aagtcaaggg gagattagtc tgggtggtgt
     2221 caatctcaat cagattgata aaaaagccct gcgccagtac atcaactatc tgcctcaaca
     2281 gccctatgtc tttaacggaa cgattttgga gaatcttctt ttgggagcca aggaggggac
     2341 gacacaggaa gatatcttac gggcggtcga attggcagag attcgagagg atatcgagcg
     2401 catgccactg aattatcaga cagaattgac ttcggatggg gcagggattt caggtggtca
     2461 acgtcagaga atcgctttgg cgcgtgctct cttgacagat gcgccggtct tgattttgga
     2521 tgaggcgact agcagtttgg atattttgac agagaagcgg attgtcgata atctcattgc
     2581 tttggacaag accttgattt tcattgctca ccgcttgact attgctgagc ggacagagaa
     2641 ggtggttgtc ttggatcagg gcaagattgt cgaagaagga aagcatgctg atttgcttgc
     2701 acagggtggc ttttacgccc atttggtcaa tagctagaaa gaggagagga tgaaaccaga
     2761 atttttagaa agtgcggagt tttataatcg tcgttaccat aatttttcca gtagtgtgat
     2821 tgtacccatg gcccttctgc tcgtgttttt acttggcttt gcaactgttg cagaaaagga
     2881 gatgagtttg tccactagag ctactgtcga acctagtcgt atccttgcaa atatccagtc
     2941 aactagcaac aatcgtattc ttgtcaatca tttggaagaa aataagctgg ttaagaaggg
     3001 ggatcttttg gttcaatacc aagaaggggc agagggtgtc caagcggagt cctatgccag
     3061 tcagttggac atgctaaagg atcaaaaaaa gcaattggag tatctgcaaa agagcctgca
     3121 agaaggggag aaccactttc cagaggagga taagtttggc taccaagcca cctttcgcga
     3181 ctacatcagt caagcaggca gtcttagggc tagtacatcg caacaaaatg agaccatcgc
     3241 gtcccagaat gcagcagcta gccaaaccca agccgaaatc ggcaacctca tcagtcaaac
     3301 agaggctaaa attcgcgatt accagacagc taagtcagct atggaaacag gtgcttcctt
     3361 ggccagtcag aatctagcct actctcttta ccagtcctac aagtctcagg gcgaggaaaa
     3421 tccccaaact aaggttcagg cagttgcaca ggttgaagca cagatttctc agttagaatc
     3481 tagtcttgct acttaccgtg tccagtatgc aggttcaggt acccagcaag cctatgcgtc
     3541 agggttaagc agtcaattgg aatcccttaa atcccaacat ttggcaaagg ttggtcagga
     3601 attgaccctt ctagcccaga aaatcttgga ggcagagtca ggtaagaagg tacagggaaa
     3661 tcttttagac aaggggaaaa ttacggcgag tgaggatggg gtgcttcatc ttaatcctga
     3721 gaccagtgat tctagcatgg ttgcagaagg tgccctacta gcccaacttt atccatcttt
     3781 ggaaagagaa gggaaagcca aactcacagc ctatctaagt tcaaaagatg tagcaagaat
     3841 caaggtcggt gattctgttc gctatactac gactcatgat gccgggaatc aacttttcct
     3901 agattcaact attacaagta ttgatgcgac agctactaag actgagaaag ggaatttctt
     3961 taaaatcgag gcggagacta atctaacttc ggagcaggct gaaaaactta ggtacggggt
     4021 ggaaggccgc ttgcagatga ttacgggcaa gaaaagttat ctacgttatt atttggatca
     4081 atttttgaac aaagagtaat gttcgtgttt ttagagttaa ataattttta aactgtgaga
     4141 aagattcttc ttgcagtttt ttctttacaa tttttgaaaa acatctacta tttattcggt
     4201 taaattcttg tgttttttgg ttttttgtgg taaaatttgc tcaagtaata cgaaaggcga
     4261 actttaaaat gtcaaaacaa ttgatctatt cgggaaaagc taaagatatc tatacaactg
     4321 aggatgaaaa tcttattatt tcaacttaca aggaccaggc gactgctttc aacggtgtca
     4381 agaaggagca gattgcaggt aagggagtct tgaataatca gatctcatct tttatttttg
     4441 agaaattaaa tgcggctggt gtggcgactc actttgtgga gaaactttca gacacggaac
     4501 aactcaataa aaaggttaag attattcctt tggaagtcgt gctccgcaac tatactgctg
     4561 gttccttttc aaaacgtttt ggtgtggatg agggaatcgc cttggagact ccgattgtcg
     4621 aattttacta caaaaatgat gatttggatg atccatttat caatgatgag catgtgaaat
     4681 tcctacagat tgcgggtgac cagcagattg cctacttgaa ggaagaaacg cgtcgtatca
     4741 atgaactatt gaaagtctgg tttgctgaga ttgggcttaa attgattgac tttaagctag
     4801 agttcggttt tgacaaggat ggcaagatta tcttggcaga cgaattttca ccagataact
     4861 gccgcttgtg ggacgctgat ggcaaccaca tggataagga tgttttccgt agaggattgg
     4921 gagaactaac cgacgtttat gagattgttt gggaaaagtt gcaggaattg aaataatctg
     4981 tttgcaacgg aaaaccttcg tctctcaact aaaaggactc aggctgaaaa ggtcccccag
     5041 accttttcac tctgtagaga actaggtgaa ctaacagatg tttacgaaat tgtctgggaa
     5101 aagttgcagg gtttaaaata acaacctcaa ggctgtttgg gaatattgca agagctgaaa
     5161 taaaggaata agaattgatg gataaacgta tttttgttga aaaaaaggct gattttcagg
     5221 tcaagtcaga gagtttggtt agagagctcc agcacaactt gggactgtca agcttgaaaa
     5281 gtattcgtat tgtgcaagta tatgatgtat ttgacttggc tgaggacttg tttgcacctg
     5341 cagagaagca cattttctct gagcagggaa ccgaccatgt tttagatgaa gtatctgtgc
     5401 aggcggatct tgctaactat gctttctttg ccattgaaag tctgccaggg cagtttgacc
     5461 agcgtgcagc ttcgtcacag gaagccttgc ttttgttggg aagttcgagt gacgtgacag
     5521 tcaacacagc ccaactttac ttggtgaata aagatattga tgcgactgag ttggaagctg
     5581 tcaaaaacta cctgctcaat ccagttgatt ctcgtttcaa ggatatcacg acagggattg
     5641 ccaagcagga gttttcagag tcagacaaga ccattccaaa attgactttc tttgaaagct
     5701 atacagcaga agactttgct cgctacaagg ccgagcaagg gatggccatg gaagtggatg
     5761 atttgctctt tatccaagac tactttaagt caatcgggcg cgtgccaact gagactgaac
     5821 tcaaggtttt ggacacttac tggtctgacc actgccgtca tacgactttt gagacagagt
     5881 tgaaacacat cgacttttca gcttctaaat ttcaaaagca attgcagtca acctatgaca
     5941 agtatattgc catgcgcgag gagttaggtc ggtctgaaaa accacaaacc ttgatggata
     6001 tggcgactat tttcggtcgt tatgagcgtg ctaacggacg attggatgat atggaagtct
     6061 ctgacgaaat caatgcctgc tcagttgaaa ttgaagtgga tgttgatggt gtcaaggaac
     6121 cttggctcct catgtttaaa aatgaaaccc acaaccatcc aacagaaatt gagccatttg
     6181 gtggagcggc tacctgtatc ggtggagcta ttcgtgatcc gttgtcaggc cgttcctatg
     6241 tttaccaagc catgcgtatt tcaggtgctg gtgatattac agcaccgatt tcggaaactc
     6301 gtgctgggaa attgccacaa caagtcattt ctaaaacagc agctcatggt tattcttcat
     6361 atggtaacca gattgggctt gccacaacct acgttcgtga atacttccac ccaggctttg
     6421 tagctaaacg tatggaactt ggtgccgttg ttggtgcggc tcccaagggc aatgttgtcc
     6481 gtgaaaaacc tgaagcaggt gatgtgatca tccttctcgg aggcaaaaca ggtcgtgatg
     6541 gtgtcggtgg tgcgacgggc tcttctaagg ttcaaacagt tgagtctgta gagactgctg
     6601 gtgctgaggt tcaaaaagga aatgccatcg aagaacgcaa gattcagcgc ctcttccgta
     6661 atggcaatgt cactcgtctg atcaagaagt ccaatgactt tggggcaggc ggcgtctgtg
     6721 tggctatcgg tgaattggca gacggtcttg aaatcgacct caacaaggtg cctcttaaat
     6781 accagggctt gaatggtaca gaaattgcca tctctgaatc acaagaacgg atggcggtcg
     6841 tggttcgtcc tgaagatgtg gatgccttcg ttgccgaatg taacaaagaa aatattgatg
     6901 ctgttgtggt agcgacagta actgaaaaac caaatcttgt catgcactgg aatggtgaga
     6961 caatcgttga cttggagcgt cgtttccttg acaccaatgg tgtgcgcgtg gttgtcgatg
     7021 ccaaagttgt ggacaaggat gtcaaactcc cagaagagcg tcaaacatct gctgaaacac
     7081 tggaatcaga tacccttacg gttctatctg acctcaacca tgcaagtcaa aaaggattac
     7141 agactgtctt tgactgctct gttggacgct caacggttaa tcacccactt ggtggtcgtt
     7201 accaactcac accaactgag gcatctgtgc agaaattgcc agttcaacac ggtgtgactc
     7261 atactgcgtc ggtcattgct caaggtttca acccatatgt agctgaatgg tctccatacc
     7321 acggtgctgc ttacgcggtt atcgaagcaa ctgctcgttt ggtggctgct ggtgccaact
     7381 ggtctaaggc tcgtttctct taccaagaat atttcgagcg gatggacaag caagcagagc
     7441 gttttggtca gccagtagcc gcccttctag gctctattga agctcagatt caacttggct
     7501 tgccatctat cggtggtaag gactccatgt ctggtacctt tgaagaattg accgttccgc
     7561 caaccttggt tgcctttggg gtgacgacgg cagatagccg taaggtgctc tctccagaat
     7621 ttaaagctgt tggggaaaat atctactaca tcccaggtca agccctctct gcagagattg
     7681 attttgactt gattaagaaa aattttgctc agtttgaagc aagccaagct gaccataaag
     7741 tgacatctgc atcagctgtc aaatacggtg gtgtagttga aagtttggct cttgctacct
     7801 ttggaaacta tattggtgca gaggtgacct tgcctgaact taaaacagct ttgacagctc
     7861 aattaggcgg ctttgtcttc acatctcctg aagaaattgc tggagtagag aagattggac
     7921 aaacgaaagc agactttaca ctgactgtca acggtgtgaa gctagatgga cacaagcttg
     7981 acagtgcatt tcaagggaca ttggaagaag tttacccaac agaatttacc caagcgaaag
     8041 aactagaaga agtaccagct gtggcatcag atgttgtgat taaagccaaa gaaaaggttg
     8101 aaaaacctgt ggtttacatc ccagtctttc caggaaccaa ctcagaatac gattcagcta
     8161 aggccttcga aaaagaaggt gcagaggtca atttggtgcc attcgtgacc ttgaatgaag
     8221 aagctattgt caagtcagtt gaaactatgg ttgacaatat cgacaagact aatattctct
     8281 tctttgccgg tggattctcg gctgcggatg aaccagatgg ttcagctaag tttatcgtca
     8341 atatcctgct taatgaaaaa gtgcgtgtgg ctattgatag ctttatcgcc cgtggtggct
     8401 tgattatcgg tatttgtaat ggattccaag ccttagtcaa atcgggtctc ctaccatacg
     8461 gaaactttga agatgctaac agtactagtc caaccctctt ctacaatgat gccaaccaac
     8521 acgtggccaa gatggtggaa actcgcattg ccaataccaa ctcaccatgg ttggctggtg
     8581 tgcaagtggg cgatatccac gctattcctg tttcgcacgg tgaagggaag tttgtcgtga
     8641 cggctgagga atttgcagag ctccgtgaca atggacaaat tttcagccaa tacgttgact
     8701 ttaacggtaa accaagtatg gattctaagt acaatccgaa tggttctgtc catgccatcg
     8761 aaggaattac cagcaagaat ggtcaaatca tcggtaagat gggccactca gaacgttatg
     8821 aggatggtct tttccaaaat atcccaggca ataaagacca acacctgttc gcatcagcgg
     8881 ttaaacattt cactggaaaa taagacttac agattttcta atagatagta tcagtaatgt
     8941 aaaagtcatg taaatctagc tcttgatgat tacaaatgaa aattaggtat aaaaaatgac
     9001 atacgaagta aaatctctta atgaagaatg tggtgttttc ggtatttggg gacatccaga
     9061 tgctgctaag ttgacctatt ttggactcca cagtcttcaa caccgtggtc aggagggggc
     9121 aggaatcctc tccaacgatc aaggacaact gaagcgccat cgtgacatgg ggcttttatc
     9181 agaagttttc agaaatccag ctaatttgga taaattgaca ggagctggtg cgattgggca
     9241 tgtgcgttat gcgactgctg gcgaagcttc tgtagataac atccagccct tcctcttccg
     9301 ttttcacgat atgcagtttg gcttggctca taatggaaat ctgaccaatg cagcctctct
     9361 caagaaagaa ctggaacaaa gaggagcaat tttcagcgcg acttcggact ctgaaatctt
     9421 ggctcacctc attcgtcgca gtcataatcc tagcctgatg ggcaaaatca aggaagcgct
     9481 cagccttgtc aaaggtggtt ttgcctatat cttgctgttt gaggacaagt tgattgcggc
     9541 tcttgaccct aatggctttc gtccgctttc tatcgggaaa atggccaacg gagcggtggt
     9601 tgtttcctct gaaacctgtg cttttgaggt cattggtgcc gagtggattc gtgatttgaa
     9661 gccaggtgag attgtgatca ttgatgacga gggcattcag tatgacagct atacagatga
     9721 tacccagttg gcgatttgtt ctatggagta tatctacttt gcccgccctg attctaatat
     9781 ccacggtgtc aatgtccata cggcacgtaa gagaatggga gcgcaattgg cgcgagaatt
     9841 taagcatgag gcagatattg tagttggtgt gcccaattct tccctaagcg cggctatggg
     9901 atttgcggaa gaatcaggct taccaaatga aatgggtctg atcaaaaacc aatacaccca
     9961 gcgaactttt atccaaccga ctcaagaatt gcgggagcaa ggagtgcgga tgaaactgtc
    10021 tgctgtttcg ggtgttgtca aaggcaaacg tgtggtcatg gtggatgatt ccattgtacg
    10081 tggaacaacc tctcgtcgta tcgttcagct cttgaaagaa gcgggtgcga ctgaggttca
    10141 cgttgccatt ggaagtcctg cactagcgta tccatgtttc tacgggattg atatccagac
    10201 ccgtcaggag ctgattgcag ccaatcatac ggtcgaagaa actcgccaaa tcattggtgc
    10261 ggacagtctg acttatcttt caattgatgg cttgattgag tcgattggta tcgaaacaga
    10321 tgcgccgaac ggtggtctct gtgtcgctta ctttgacggt gactacccaa cgcctctcta
    10381 tgactacgaa gaagactatc gtagaagttt ggaagaaaag accagttttt acaagtaggc
    10441 gacagattct ccattaaaga aaaggaaaaa acaaatgaca aataaaaatg catatgccca
    10501 gtcgggtgtg gatgttgaag cgggttatga agttgttgaa cggattaaaa agcacgtggc
    10561 ccgtacggag cgtgcaggtg tcatgggagc tcttggtggc tttggtggta tgtttgacct
    10621 ttccaagact ggggttaaag aacccgtctt gatttcaggg actgacggtg tcggaaccaa
    10681 gctcatgttg gctatcaagt acgacaagca cgataccatc gggcaggact gtgtggccat
    10741 gtgtgtcaac gacatcattg ctgcaggtgc ggaacccctc tattttctcg actacgtagc
    10801 gacagggaag aatgaaccag ctaagctaga acaagtggtt gctggtgtgg cagaaggttg
    10861 tgtgcaggct ggtgctgccc tcatcggtgg ggaaacggct gaaatgccgg gcatgtacgg
    10921 cgaagacgac tatgacttgg ctggttttgc ggtcggtgtg gctgaaaaat ctcaaatcat
    10981 tgacggttca aaggtggtag agggagatgt tcttctcgga cttgcttcaa gtgggattca
    11041 ctcaaatggt tactctttgg ttcgtcgtgt ctttgcggat tacacaggtg aggaagtcct
    11101 accagaattg gaaggcaaga aacttaagga agttctactt gagccgactc gtatctatgt
    11161 caaggctgtc ttgccgctca tcaaagaaga gttggtcaac ggcattgccc acatcacagg
    11221 tggtggcttt atcgaaaatg tccctcgtat gtttgcagat gacctagctg ctgaaattga
    11281 tgaaagcaaa gttccagtgc ttccaatttt caaagccctt gaaaaatatg gtcagattaa
    11341 acacgaagaa atgtttgaaa tcttcaatat gggtgtggga cttatgttgg cggtcagccc
    11401 tgaaaatgta gagcgtgtaa aagaattgtt ggatgaagca gtctatgaaa ttggtcgcat
    11461 cgtcaagaaa gaaaacgaaa gtgtcattat caaatgaaaa aaatagcggt ttttgcctct
    11521 ggtaatggct caaattttca ggtgattgcc gaagaatttc cagtggagtt tgtcttttca
    11581 gaccatcgtg atgcctatgt gcttgagcgt gcaaagcagc tcggcgttct gtcctatgct
    11641 tttgaactca aggagtttga gagcaagaca gactacgaag cagcccttgt cgaactcttg
    11701 gaagaacacc agattgactt ggtttgccta gcaggctaca tgaaaatcgt tggaccaacc
    11761 ttattgtcgg cttatgaagg tcggattgtc aacattcatc cagcctactt gccagaattt
    11821 ccaggagctc atgggattga ggatgcttgg aatgctggcg tgggtcagtc tggtgtgacc
    11881 attcactggg tggattcggg tgtggataca ggccaggtca tcaaacaggt tcgtgtgcca
    11941 cgactagctg atgataccat tgacagattt gaagctcgca tccatgaagc agagtacagg
    12001 ttgtatccgg aagtagtgaa ggctctattt acagattgac tttttgatga ttcatatgat
    12061 atctttgatt ttaaattgga gtcagtgttt gttgaagacg gcttcaaacg gaggtatttg
    12121 taatgttaga atctaaaaaa acaactcgat atgtatttta tgtctatctg atgttattaa
    12181 cttggggaat cttatttaag tttgaaacaa atcctgaatt tatagcattt ttcttagctc
    12241 caaggtatat caattggatt ccattttcag aaccactaat agtcgatgga aaaattgttt
    12301 ttgctgaaat gttatttaat ctgatttcct ttattccatt aggtgtttgt ttccctttga
    12361 taaaaactaa tttatctagt ttaagaatag tcgggacagg tttcttgatt agtttattgt
    12421 ttgagtgctt acagtatatt ttagcaatag gtataacaga tataacggat ttgactttaa
    12481 atacgctagg tgtctgtgta ggcttactga tttatcaaat ttttataaga gtgttcaaat
    12541 cacagactag aaaatggatc aatatcttag gtatgcttag ccttggtttt gcttatcttg
    12601 ttttactgtt actgcattta attggtgttt aactaatgat taaaaaggag aatataatga
    12661 ctaaacgcgt cttaatcagc gtctcagaca aagcgggcat tgttgaattt gcccaagaac
    12721 tcaaaaaact tggttgggag attatctcaa caggtggaac taaggttgcc cttgataatg
    12781 ctggggtgga taccattgct atcgatgatg tgactggttt cccagaaatg atggacggtc
    12841 gtgtgaagac cctccaccca aatatccacg gagggcttct cgctcgtcgt gacttggata
    12901 gccacttgga agcggctaag gacaacaaga ttgagctcat tgaccttgtg gtggtcaacc
    12961 tttacccatt taaggaaact atccttaaac cagatgtgac ttatgctgat gcagttgaaa
    13021 atatcgatat tggtgggccg tctatgcttc gttcagcagc gaaaaatcat gccagtgtta
    13081 cagttgtggt agatcctgct gactacgctg tggttttgga cgaattggca gcaaacggcg
    13141 aaacctctta tgaaactcgc caacgtttag cagccaaagt atttcgtcac acagcggctt
    13201 atgacgcctt gattgcagaa tacttcacag ctcaagtggg tgaaagcaag cctgaaaaac
    13261 tcactttgac ttatgacctc aagcaaccaa tgcgttacgg tgagaatcct caacaagacg
    13321 cggactttta ccagaaagct ttgcctacag attactctat tgcttcagcc aaacagctca
    13381 acgggaaaga attgtcattt aataatatcc gtgatgcaga tgctgctatc cgtatcatcc
    13441 gtgacttcaa agatagtcca accgttgtgg ctctcaaaca catgaatcca tgtggaattg
    13501 gtcaagctga tgacatcgag actgcttggg actacgctta tgagtctgac ccagtatcta
    13561 tctttggtgg gattgtcgtc ctcaaccgtg aggtggatgc tgcgacagct gagaagatgc
    13621 acggcgtttt cctcgaaatc atcattgcac caagctatac ggatgaagcg ctagccattt
    13681 tgatcaataa aaagaaaaac ttgcgtatcc ttgccttgcc atttaatgct caagaggcta
    13741 gcgaagtgga agcagaatac acaggtgtag tcggtggact tctcgtgcaa aatcaagacg
    13801 tggtcaagga aagcccagct gactggcaag tggtgaccaa acgtcagcca accgagacag
    13861 aagcgactgc tcttgagttc gcttggaagg ctatcaagta cgtcaaatca aatggtatta
    13921 tcgtgaccaa cgaccacatg acacttggtg ttggtccagg tcaaaccaac cgtgtggctt
    13981 ctgttcgcct tgccattgac caagccaaag atcgtctgaa cggggcggtc cttgcttcag
    14041 atgccttctt cccatttgcg gataacgtgg aagaaatcgc caaagcagga attaaggcca
    14101 tcatccagcc cggtggctct gtccgtgacc aagaatccat cgaagcagcg gataaatacg
    14161 gcttgactat ggtctttaca ggtgtgagac attttagaca ttaagaagat aaaagggaag
    14221 aaaacagttt ctttcctttt ttggcttaaa atgctaactg aaacaagatt aaaacgaact
    14281 tttttgatat aatgttggta aataattcgc aaaagaggtt gaggaatgaa actgcttgtt
    14341 gtcggttctg gtggtcgtga gcatgcgatt gctaaaaagt tacttgaatc aaaagacgtg
    14401 gaaaaagtct ttgtagctcc tgggaatgat gggatgactc tggatggttt ggaattggta
    14461 aatatctcta tttccgaaca ttctaaattg attgacttcg caaagaccaa tgatgttgct
    14521 tggaccttta tcggtccaga tgatgccctt gctgctggta tcgtggatga ttttaaccaa
    14581 gctggactta aggcctttgg tccgactagg gctgcagcgg agctggagtg gtccaaggat
    14641 ttcgccaagg aaatcatggt caaatacggc gttccgacag caacatatgg cacattttca
    14701 gatttcgagg aagccaaggc ctatatcgaa aagcatggtg cgcctatcgt agtcaaggcg
    14761 gatggcttgg cacttgggaa gggtgtcgtc gttgctgaga cggttgagca agcggtcgaa
    14821 gccgctcatg agatgctttt ggacaataaa tttggtgact caggtgcgcg cgtggttatt
    14881 gaggaattcc ttgaaggaga ggaattttca ctctttgcct ttgtcaatgg tgataagttc
    14941 tacatcatgc caacggctca ggaccacaaa cgtgcctatg atggcgacaa agggcctaac
    15001 acgggtggta tgggtgccta tgcgccagtc ccacacttac cacagagtgt agttgataca
    15061 gcggttgaca ccattgtcaa gccagttcta gaaggggtga ttaaagaagg tcgcccttat
    15121 ctgggagttc tttacgcagg gcttatcctg acagctgatg gaccgaaagt cattgagttc
    15181 aacgctcggt tcggagatcc agaaactcag attatcttgc ctcgcttgac ctctgacttt
    15241 gctcaaaata tcacagatat cctggatagc aaggagccaa atatcatgtg gacggacaag
    15301 ggtgtgactc tgggtgtggt tgtcgcatcc aagggctacc cgctagacta tgaaaggggc
    15361 gttgagttgc cagccaagac agaaggcgat gtcatcacct actatgcagg ggctaagttt
    15421 gcggaaaata gcagagcact gctctcaaac ggcggacgag tttatatgct cgttaccata
    15481 gcagataccg tcaaagaagc ccaagccagc atataccaag aactatacca acaaaaaata
    15541 gaaggactct tctaccgaac agatatcgga agcaaggcaa ttaagtaaag atataagaat
    15601 aacgcgccgt agtcgccaaa cacgataatg gtcgtcgtgg tgaaaagacc agaacagtga
    15661 atgttctggt cagggggaaa cttggagacc ttaggctcaa agtttaggaa tgaaaccgaa
    15721 ggtttgcttc cgcctccatc acctaagacc attatcaaaa agaaaaataa aaattcacaa
    15781 aatacgttaa tgatcgtatg gtttgcgagc gttagcgagc taatatagaa caatcaccgc
    15841 cgttgtgaaa gaacgattgg atgataatcc aatcgttcag ggaaattgga agaccttggg
    15901 tttccaattt aggcatgaga cacctttggt ggctgctgcc gtccctcaca agctaaggtg
    15961 attgttgaaa aagaggaaaa aggagaagaa atgaaaccag taatttccat catcatgggc
    16021 tcaaaatccg actgggcaac catgcaaaaa acagcagaag tcctagaccg cttcggtgta
    16081 gcctacgaaa agaaagttgt ttccgcacac cgtacaccag acctcatgtt caaacatgca
    16141 gaagaagccc gtagtcgtgg catcaagatc atcatcgcag gtgctggtgg cgcagcgcat
    16201 ttgccaggca tggtagctgc caaaacaacc cttccagtca ttggtgtgcc agtcaagtct
    16261 cgtgctctta gtggagtgga ttcactctat tctatcgttc agatgccggg tggggtgcct
    16321 gttgcgacca tggctatcgg tgaagctgga gcgactaacg cagctctctt tgccctccgt
    16381 ctcctctctg tagaagataa gtccattgcg gatgcacttg ccaactttgc tgaagaacaa
    16441 ggaaaaatcg cagaggagtc gtcaaatgag ctcatctaaa acaatcggaa ttatcggtgg
    16501 cggtcaactg ggtcagatga tggccatttc tgctatctac atgggccaca aggttatcgc
    16561 gctggatcct gcggcggatt gcccggtctc tcgtgtggcg gaaatcattg tggcacctta
    16621 taacgatgta gacgccctcc gtcagttggc agaccgttgc gatgtcctca cttacgagtt
    16681 tgaaaatgtc gacgctgacg gtttggatgc cgttatcaag gatggacaac tccctcaagg
    16741 gacagacctg ctccgcattt cgcaaaatcg tatttttgaa aaggactttt tgtcaaacaa
    16801 ggctcaagtc accgttgcac cttacaaggt tgtgacctca agccaagatt tggcagatat
    16861 cgacttgtcg aaaaactatg ttctcaagac tgcgactggt ggctacgatg gtcatggaca
    16921 aaaggttatt cgttcagaag cagacttgga agcagcctat gcgctagcag actcagcaga
    16981 ctgcgtcttg gaagaatttg tcaactttga ccttgagatt tctgtcatcg tgtcaggaaa
    17041 tggcaaggag gtgacgtttt tcccagttca ggaaaatatc caccgcaaca atatcctgtc
    17101 taagaccatt gtaccagccc gtatttctga aagtctagca gacaaggcta aagctatggc
    17161 agtgcgaatc gcagaacaac tcaacttgtc tggaactctc tgtgtggaaa tgtttgcgac
    17221 agctgatgac atcattgtca atgaaatcgc cccacgacca cataactctg ggcactattc
    17281 tattgaagcc tgtgatttct ctcagtttga cacccatatt ctgggtgttc tcggagcacc
    17341 attaccagtc atcaaactcc atgcgccagc cgttatgctt aatgtcctcg gtcagcatgt
    17401 cgaggctgct gaaaaatatg tcacagaaaa tccaagcgcc cacctccaca tgtatggtaa
    17461 aatagaagca aagcataatc gtaagatggg acatgtgact ttgtttagtg atgtgccgga
    17521 tagtgtggaa gagtttgggg aagggattga tttttaggac aagtctatga tacaaattat
    17581 cgttaataca tttattgaaa agtataagac tggagcagtt gttgaagtgt tgtatgccag
    17641 tgctgaccaa gataaggtac aagctaaata tgaagaacta gctgcacaat accccgaaaa
    17701 ttatttagct atctatgatg taccgctgga tacggatttg aatacactag atcattaccc
    17761 gtctgtgttt attgaaaaag aggagtttga gtagaaatct tggtttacct agatagctta
    17821 ttcccaacag cttaagaaga aaggaaaaat taacacatga tcaaccgtta ctctcgccct
    17881 gagatggcga atatttggag tgaagaaaat aaataccgtg cttggcttga ggtggaaatc
    17941 ctctctgacg aggcatgggc tgagttgggg gaaatcccta aggaagatgt ggctttgatt
    18001 cgcaagaagg cggactttga catcgaccgt attttggaaa ttgagcagga gacgcgccac
    18061 gatgtggtgg ctttcacgcg tgcggtttct gagactcttg gtgaagagcg caagtgggtt
    18121 cactatgggt taacttctac cgacgtggtg gatactgctt atggttacct ctacaagcag
    18181 gccaacgaca tcatccgtcg tgaccttgaa aacttcacta atatcatcgc tgacaaggcc
    18241 aaggagcaca agttcaccat catgatgggg cgtactcatg gtgtgcacgc tgagccgaca
    18301 acttttggtc ttaaattggc aacttggtat agcgaaatga aacgcaatat cgagcgtttc
    18361 gagcatgcgg ctgctggtgt agaagctggt aagatttctg gtgcggttgg gaactttgcc
    18421 aatatcccac catttgtaga ggagtatgtc tgcaacaagc ttggtatccg tgctcaagaa
    18481 atctctacac aagtccttcc tcgtgacctt cacgctgagt actttgcagt tcttgccagc
    18541 attgcgactt caatcgaacg tatggcgact gagattcgtg gtctacaaaa atctgagcaa
    18601 cgcgaagtag aagagttctt tgctaaaggg caaaaagggt cttcagcaat gcctcacaaa
    18661 cgcaacccaa tcggttctga aaatatgact ggtctggcgc gtgtcattcg tggtcacatg
    18721 attacggctt atgaaaacgt cgctctctgg cacgaacgcg atatttccca ctcatcagct
    18781 gagcgtatca tcacaccaga tacgaccatt ttgattgact acatgctcaa ccgttttgga
    18841 aatatcgtca agaacttgac agtcttccca gaaaatatga tccgaaacat gaactcgact
    18901 tttggtctta tctttagcca acgggctatg ttgacattga ttgaaaaagg catgacccgt
    18961 gagcaagcct atgacttggt gcaaccaaaa acagcctact cttgggacaa ccaagtagac
    19021 tttaaaccac ttcttgaggc agattcagaa gtaacatcac gtctcactca agaagaaatc
    19081 gatgaaatct tcaacccagt ttattacacc aaacgagtgg atgatatctt tgaacgtctt
    19141 ggactaggtg attaattaaa aaataaacag cgagcttcaa tctcgctgtt tattttttat
    19201 cgaaaagact tagtcttctt ttcttttagt gagtccatag gctgctagtg tggacatgag
    19261 tcctgcgact actagttctg cagaatcgtg agttcctgtt tcaggaagtt ttttctctgt
    19321 taccacagga gctggatctt gaggaagagc tttgctttcc tcagcaggag cagttgatgg
    19381 agctggttgg cttgggattt ctagttttgg tttttcttca gcaatagcgg cttgtccgtt
    19441 ttcatcgcct acatgtgtta ccatagttcc gacttcgact atttgagtaa cggcttcctg
    19501 tgctacgaca ctatttacaa gtgttttcac ttccttacca tcggcagaag tgctcacaga
    19561 gtagaagttg ctacgatgtc cattgacgcc cttagtaatg acttgtgttt ttcctttgag
    19621 taagagtgga ttttcacgag tcactgtggt aaatggaatt tcttcttctt ggatatccag
    19681 tctaggtttt acctcagtag ttggtgcaag accactttca tcacccttgt gagttacagg
    19741 agagccaact tcaaccactt ggtttataac ttctttggtt acctggctat caaggactgt
    19801 ttctgttgtt tttccatttt cagtgagtac agagatgtaa tgagttcgtt cacctttgac
    19861 tcctgctgtg ataatatttt cctgaccagc tgggaggtta ggattttctt tcttgataac
    19921 ttcaaatgga atttcttcag ttcttgtgat gagttctggt ctggtttcaa cattggcagc
    19981 cacttcattt tcatctaggc ttcctgaatg agttgcagct ggtttgaggc cttgaagagc
    20041 ggcttttagg ttggctacaa gtgtgtcaag ctcagcttgt ttattacggt tgaggttgta
    20101 atttagagct gttttagctg cgtcaagggc ctcaagactt tctttactat atccttctaa
    20161 gtttgtagga attttagcta attcttcgcg gagagcatta taattagcac ggaagtagtc
    20221 tttgttgtgg tctgcaaagg cagtcatgag ttcaaagatt tcctcttcct tgtactcagc
    20281 gcttggtcta tctgcccaga ttgaaagcat acttccgact gttggaagat ctacttcagg
    20341 atatttggta gaagctagtt gattgaatgg aactttacca gaattttcaa ctgcttttga
    20401 aagagggtaa gcttcatctt gtttgtgatt gcctataaca taataccagt caccgttggt
    20461 attcaagaat ttatagcctt tgcttgctag gtattgaggt gatgcgaggt tatatcccca
    20521 ccagccttta gaccagtaag aaatcaagac atctttgtca aactgaacat cgtccttgtc
    20581 ttcatagtag aagccatcgt tgaaggccat tggttgaagc cctctttctt tggccatagc
    20641 tgcgagggtg ttggcatatt cggcaaattt gccatagagt tgataccact tgaggtagta
    20701 ccagccttgg gcactagtcg catcgttggc gtattcgtca gtaccaaagt tgaaaatctt
    20761 tgttttacct gcaaagaagt ccatgtattt accgatgagg gcttttacaa agttcatcgc
    20821 ttcttcgttt ttcaagtcca tagttgtttt tgaaacttta tcaaagtggg cttgaggatt
    20881 tttaatacct aatttttcca tggcaaccag catagcatcc atgtgacctg gactgttaat
    20941 agctgggatg agaccaatgt ccttagattt agcgtattca attagctctg tcacttctgc
    21001 ctgtgttagt gcagtaccgt ttggatcgtc gtagtaagct ttagttcctt cgataatagc
    21061 ttttttaacg tcatcactag cataggtttt tccgttggca gtaatggtca tatcattgag
    21121 tagaaagcga agtccgtcat ttcctagaag gagatggaca tcagaatatc cgagctcact
    21181 ggccttgtct acgatgcgtt tgagctggtc cagagtaaag tatttgcgtc cagcatcgat
    21241 tgagattacc ttgtttttgg caagtttttc aacctcacgt ttagcttctt cttctttttg
    21301 agcttcaggc gtgagggtca agttgttgac agtttcttga agtttagcaa tggcttgatc
    21361 aatcgtatct tgttgggcac ggctaaggtt gctatcgaga gagcgaatag ctttttcagc
    21421 ttcttttacg gccgcgacgc tttctgcagt ataacggttc aggtcttttg gtacctcgtt
    21481 aagtgcttgc tctgcagatt cataatcagc tgcgaagtat tcagcgttgg catttgcaaa
    21541 atgacgcatg agtttgaaga ggtgtgatgg tgaataacgt gcagatggag tgtcagccca
    21601 agcagctacc ataccaccga tgattgggat atcagctcct tctgtttttg gtacagaagt
    21661 gattggtgtg tttttaatac cattgagccc ctgatcgaga ttgtaccagc cttggccatc
    21721 agcgtttcgt ccaagaacgt agtaccaagc atcattggta ttaaggattt ggtgaccttt
    21781 ttcagctagt agtttagaag aagcgacatc gtagcctccc caaccaccag tccacataga
    21841 aacgatgatg tctttgtcaa aactaccaaa gcttgtgtcg ctattgtagt agataccgtc
    21901 gttaaaggcc attggtttga gaccgtgcga ttttacaata cgagcgaggt cattggcgta
    21961 ggcaataaat ttttcatagc cttttacagg gtagccttcg tttggatagt atttatcagc
    22021 ttgaagcaca ctccaacctt tagcatctgt cgcatcattg gcatattcat caagtccgat
    22081 gttgaagatt tcagtctttt tcgcgaaata agcagcatac ttgtcgataa gggcttttgt
    22141 aaaagcgaca gcttgttcgt tgtcaagatc gacagtacgg gctgatttct tcccaaaata
    22201 gctaaagtta gggttttgga ttcccaattc tttcatggca ttgagaatcg catccatgtg
    22261 tccaggacta tttactgtcg gaatgagacc gataccttta tctttggcat agttaatcag
    22321 atctgtcatt tgactttctg ttaagtgatt gccgtttgga tcgttgtaat aatcatttgt
    22381 acctttttca atggcgcgtt tgacatcgtc actggcatag gtcttgccgt tagctgtgat
    22441 gctcatatcg tccaacatga aacggagtcc atcatttccg actaataggt gtaaatcagt
    22501 gtagccataa tgtttcgctt tatcgatgat ttccttgagc tgttctggtg agaaatattt
    22561 acgtccagca tcaatagaaa caattttctt tttcgctagt ttttcattta cagttgcagc
    22621 acgttccttt cctgcctctg ttgccggttt gtcagcctct gctttcgctt catctttttt
    22681 agctggttta tccttgtcag tcttgtctgt atttgactct ttagaatcaa cctctttcgc
    22741 ttcttccttt ttagggctag cttcttctgc ctttttatta gcagtttctt tttcagcaga
    22801 agttggagtt accacttctg ctttatcact aggagttgaa ctaacttcct cttgtggttt
    22861 ttcttctgtt tttggaagac tagctacctt atcagtagct ggagtttctg tttctacagt
    22921 ttttggagct tctggttgaa gcactgcttt aggtgtttcc tcagtccgat tttcggatga
    22981 ttgaggggaa tcagaaaccg tatggatggt cggttggttt tctgtagtag taggagtaac
    23041 tccatcggct gcaacagtct gtgcttggaa ggcaaatcca attagaacag aagctgctcc
    23101 tacagcgtat ttacgaatag aaaaacgctg ttgtttttca tgtttcattg caaaacctcc
    23161 tgattgcatt gttatattga tagcgattat ataaatcaac gcctttattt tatttcttat
    23221 attaacgaga gtcaagagga gatgacaaaa aactataata agtataaaaa aatataaaat
    23281 ttaaacttaa gatttcaga
//
LOCUS       DAVKWZ010000031        23041 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000031 DAVKWZ010000000
VERSION     DAVKWZ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23041
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.031"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(<1..2315)
                     /locus_tag="VW854_001245"
     CDS             complement(<1..2315)
                     /locus_tag="VW854_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RICH domain-containing protein"
                     /protein_id="HEV0262480.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVVVASLVMGSVVHATENEGIT
                     QVATSYNKANESQTEHRKAAKQVDEDIKKMLSEIQEYIKKMLSEIQLDKRKHTQNVNL
                     NRKLSAIQTKYLYELRVLKEKSKKEELTSKTKKELDAAFEQFKKEPELTKKLAEAKQK
                     AKAQKEEDFRNYPTNTYKTLELEIAEFDVKVKEAELELVKEEAKPRNEEKIKQAKAKV
                     ESKKAEATRLEEIKTERKKAEEEAKRKAEESEKKAAEAKQKVDTKEQGKPKRRAKRGV
                     SGELATPDKKENDAKSSDSSVGEETLPSPSLNMANESQTEHRKDVDEYIKKMLSEIQL
                     DRRKHTQNVNLNIKLSAIKTKYLYELSVLKENSKKEELTSKTKAELTAAFEQFKKDTL
                     KPEKKVAEAEKKVEEAKKKAKDQKEEDRRNYPTNTYKTLELEIAESDVKVKKAELELV
                     KEEANESRNEEKIKQAKEKVESKKAEATRLEKIKTDRKKAEEEAKRKAEESEKKAAEA
                     KQKVDAEEYALEAKIAELEYEVQRLEKELKEIDESDSEDYLKEGLRAPLQSKLDTKKA
                     KLSKLEELSDKIDELDAEIAKLEVQLKDAEGNNNVEAYFKEGLEKTTAEKKAELEKAE
                     ADLKKAVDEPETPAPAPQPAPEKPAEKPAPAPAPEKPAPAPEKPAEKPAPAPEKPAPT
                     PETPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNSNGAMATGWLQNNGSWYYL
                     NSNGAMATGWLQYNGSWYYLNANGDMATGWF"
     gene            complement(2394..2921)
                     /locus_tag="VW854_001246"
     CDS             complement(2394..2921)
                     /locus_tag="VW854_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEV0262481.1"
                     /translation="MVLAIILVTFLIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     LAHIVFYLSCLVEAMVHKTMFDGMSMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYMTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            complement(3016..4347)
                     /gene="hk06"
                     /locus_tag="VW854_001247"
     CDS             complement(3016..4347)
                     /gene="hk06"
                     /locus_tag="VW854_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEV0262482.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGKQINGMYEHLLKVIHE
                     LESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSI
                     NKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIENQLT
                     DATRVVMSLRALDKVLTNLISNAIKYSDENGRVIISEQDGYLSIKNTCAPLSDQELEH
                     LFDIFYHSQIVTDKDESSGLGLYIVSNILESYQMDYSFLPYEHGMEFKISL"
     gene            complement(4344..4997)
                     /gene="rr06"
                     /locus_tag="VW854_001248"
     CDS             complement(4344..4997)
                     /gene="rr06"
                     /locus_tag="VW854_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEV0262483.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDIWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            complement(5639..8071)
                     /locus_tag="VW854_001249"
     CDS             complement(5639..8071)
                     /locus_tag="VW854_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV0262484.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKHVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            complement(8073..8531)
                     /locus_tag="VW854_001250"
     CDS             complement(8073..8531)
                     /locus_tag="VW854_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEV0262485.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRDLLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQVIQEVDRKGK"
     gene            complement(8650..9378)
                     /locus_tag="VW854_001251"
     CDS             complement(8650..9378)
                     /locus_tag="VW854_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0262486.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            complement(9378..10385)
                     /locus_tag="VW854_001252"
     CDS             complement(9378..10385)
                     /locus_tag="VW854_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV0262487.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            complement(10424..11182)
                     /locus_tag="VW854_001253"
     CDS             complement(10424..11182)
                     /locus_tag="VW854_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0262488.1"
                     /translation="MRNLRSILRRHISLLGFLGVLLIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     KPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRS"
     gene            complement(11145..11435)
                     /locus_tag="VW854_001254"
     CDS             complement(11145..11435)
                     /locus_tag="VW854_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEV0262489.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     regulatory      complement(11506..11608)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(11691..13037)
                     /gene="cbpD"
                     /locus_tag="VW854_001255"
     CDS             complement(11691..13037)
                     /gene="cbpD"
                     /locus_tag="VW854_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEV0262490.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEMPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRV
                     VKANTMTGFIHFKDLDGGSVGNSQSSASTGGTHYFKTKSAIKTEPLASGTVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGHSVLSSTGGTHYFKTKSA
                     IKTEPLVSATVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     gene            complement(13126..13392)
                     /locus_tag="VW854_001256"
     CDS             complement(13126..13392)
                     /locus_tag="VW854_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262491.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            complement(13394..14746)
                     /gene="dnaB"
                     /locus_tag="VW854_001257"
     CDS             complement(13394..14746)
                     /gene="dnaB"
                     /locus_tag="VW854_001257"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEV0262492.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            complement(14790..15242)
                     /gene="rplI"
                     /locus_tag="VW854_001258"
     CDS             complement(14790..15242)
                     /gene="rplI"
                     /locus_tag="VW854_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEV0262493.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            complement(15239..17212)
                     /locus_tag="VW854_001259"
     CDS             complement(15239..17212)
                     /locus_tag="VW854_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEV0262494.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENNETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKSLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            complement(17353..17901)
                     /gene="raiA"
                     /locus_tag="VW854_001260"
     CDS             complement(17353..17901)
                     /gene="raiA"
                     /locus_tag="VW854_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEV0262495.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNIVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            complement(17981..18643)
                     /locus_tag="VW854_001261"
     CDS             complement(17981..18643)
                     /locus_tag="VW854_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEV0262496.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            complement(18640..19938)
                     /locus_tag="VW854_001262"
     CDS             complement(18640..19938)
                     /locus_tag="VW854_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV0262497.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVIATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTL
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLAEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            19994..20629
                     /locus_tag="VW854_001263"
     CDS             19994..20629
                     /locus_tag="VW854_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEV0262498.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            20914..21834
                     /gene="cysK"
                     /locus_tag="VW854_001264"
     CDS             20914..21834
                     /gene="cysK"
                     /locus_tag="VW854_001264"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEV0262499.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKLGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(22031..22429)
                     /locus_tag="VW854_001265"
     CDS             complement(22031..22429)
                     /locus_tag="VW854_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0262500.1"
                     /translation="MKSPIPKAPLANNFGSASIIAHTIHQKFNLKVPNYRQEEDWARM
                     GLPITRKEISNRHIKTSQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHVVIDLFISYFNPL"
     gene            22420..22728
                     /locus_tag="VW854_001266"
                     /pseudo
     CDS             22420..22728
                     /locus_tag="VW854_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(22869..>23041)
                     /gene="tnpB"
                     /locus_tag="VW854_001267"
     CDS             complement(22869..>23041)
                     /gene="tnpB"
                     /locus_tag="VW854_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEV0262501.1"
                     /translation="KDRFKALYWDGQGFWLLYKRFENGKLTWLSTEKDVKALAPEQVD
                     WLMKGFSITPQI"
BASE COUNT         6706 a         5164 c         3818 g         7353 t
ORIGIN      
        1 tggaaccatc ctgtcgccat atcaccatta gcgttgaggt agtaccatga accattgtat
       61 tggagccatc ctgtcgccat agcgccattg ctgttgaggt agtaccatga gccattgttt
      121 tggagccagc ctgtcgccat agcgccattg ctgtttaggt agtaccatga gccattgttt
      181 tggagccagc ctgttgccat tgaaccatca gtattgtaga agtaccacat accgttttct
      241 tgtttccagc ctgtttttgg agtttctgga gttggagctg gtttttctgg agctggagct
      301 ggtttttcag ctggtttttc tggagctgga gctggttttt ctggagctgg agctggagct
      361 ggtttttcag ctggtttttc tggagctggt tgaggagccg gagctggagt ttctggctca
      421 tcaactgctt tcttaaggtc agcttcagct ttttctaatt cagctttttt ctcagcagta
      481 gttttctcta aaccttcttt aaagtaggct tctacattat tgtttccttc agcatcttta
      541 agttgaactt caagttttgc aatttcagcg tctaactcat caatcttatc actcaactct
      601 tcaagttttg atagtttagc ttttttggta tccaatttag attgaagagg agcacggagg
      661 ccttctttaa gataatcttc tgagtcagac tcatcaatct ctttgagctc tttttctagt
      721 ctctgaactt catattccaa ctcagcgatt ttagcttcaa gagcatattc ttcagcatcc
      781 actttttgtt tggcttcagc agcttttttc tcagattctt ctgcttttcg tttagcttct
      841 tcttctgctt ttttacgatc tgtcttgatt ttttctaacc ttgtagcctc agctttttta
      901 ctctcaactt tctcttttgc ttgcttaatt ttttcctcgt ttcgagattc gttagcttcc
      961 tcttttacta gttcaagctc cgctttttta actttcacat cggactcagc aatttcaagt
     1021 tcaagcgttt tgtaagtatt ggttgggtag ttacggcgat cttcttcttt ttgatccttg
     1081 gcttttttct tagcttcttc aaccttcttc tcagcttctg ctaccttttt ttctggtttc
     1141 aatgtatctt ttttaaactg ctcaaaagct gcggttaact ctgctttggt ttttgacgtc
     1201 aactcttctt ttttcgagtt ctcttttaaa acacttaatt catacaaata cttcgtttta
     1261 attgcgctca actttatgtt taagttgaca ttttgggtat gttttcttct atctaattgg
     1321 atctcactca acattttttt tatatattca tcgacatctt tcctatgttc tgtctgactt
     1381 tcatttgcca tattaaggga tgggcttgga agagtttctt cacctacgct agaatctgaa
     1441 gacttcgcat cattttcttt tttatcaggt gttgctagct ctccagaaac tcctcgtttt
     1501 gcccgcctct ttggtttacc ttgctcttta gtatccactt tttgtttggc ttcagcagct
     1561 tttttctcag attcttctgc ttttcgttta gcttcttctt ctgctttttt acgttctgtc
     1621 ttgatttctt ctaaccttgt agcctcagct tttttactct caactttcgc ttttgcttgc
     1681 ttaatttttt cctcgtttcg gggtttagct tcctctttta ctagttcaag ctccgcttct
     1741 ttaactttca catcgaactc agcaatttca agttcaagcg ttttgtaagt attggttggg
     1801 tagttacgga aatcttcttc tttttgagcc ttggcttttt gtttagcttc tgctaatttt
     1861 ttagtaagtt ctggttcttt tttaaactgc tcaaaagctg cgtctaactc tttttttgtt
     1921 tttgacgtca actcttcttt tttcgacttc tcttttaaaa ctcttaattc atacaaatac
     1981 ttcgtttgaa ttgcgctcaa ctttctgttt aagttgacat tttgggtatg ttttctttta
     2041 tctaattgga tctcactcaa catttttttt atatattctt ggatctcact caacattttt
     2101 tttatatctt catcgacttg tttagcagct ttcctatgtt ctgtctgact ttcatttgcc
     2161 ttattataag aagtggctac ttgggtaatt ccctcattct ccgtcgcatg aaccacactt
     2221 cccataacaa gactagcaac tactacacta gctactccaa tactaaattt acgaattgaa
     2281 taatgtactt ttctttcgct ttttgatgca aacatgttta tttccttcta tattttttct
     2341 ttaaccttac tatatcactt caatatgaac tgtatataaa tcgaattttg tatttatctc
     2401 tttatgcttc catttgggat tataacttca tgtaatagct tttcctcttc agctattcgt
     2461 cgatacaaaa taactgcata aactggaaaa actaaaacaa aagtcatata ggcatgactc
     2521 aacaaggtca agccaatcaa ctcaggtaaa atatttaaaa agtaattagg atgttttact
     2581 gtcctaaaca agatatgatc tacatatttg tgattattga caagcataag cttcactgtc
     2641 caaatatctc ccaacaagtg aatcaccaac atcaacatca gcatagaaaa aataagcaag
     2701 actaaaccaa ccatgctcat accatcaaac attgtcttat gcaccattgc ttcaacgagg
     2761 caagagagat aaaaaacaat atgagccaac gttaatcgtt ttgtattctc aactccaaat
     2821 tctttcccgc cattgctaag gattcgtttc tcattctcta tcgaacgctt taaaaaaatc
     2881 aatcgaataa ggaatgttac taaaataatc gctaatacca tattagtatc ctttctttta
     2941 tctatatttt tatctatata aagaataata cacttatgtt aaccctatgt gaactttaat
     3001 aaaaaactaa tctgtctaca agctaatctt aaattccata ccgtgttcat aagggagaaa
     3061 actatagtcc atttgatagc tttctaaaat attactcaca atgtaaagac ccaaaccgga
     3121 actttcatcc ttatctgtca cgatttgaga atgatagaat atatcaaata aatgttctag
     3181 ttcttggtca cttagaggcg cacatgtatt cttgatagag agatagccat cttgctcgga
     3241 tatgattacg cgcccatttt catctgaata tttaattgca ttactaatca ggtttgtcaa
     3301 aaccttatcc aatgccctaa gactcatgac gaccctggta gcatctgtca attgattttc
     3361 aattgtaaca cctattgaat gagctaattc ttgataacgt gataaaatat ctactaaaac
     3421 tggcttaaca gtcaaggtct cacgacactc tgtccactct tggaatttag aagactccag
     3481 tacttcttcc aataagtggc tcatctggtc aatcttattt atactctttg caatatattt
     3541 tggatgatct ttgtaatctc caatattatg ctgcatattc tctaggataa ttctaagact
     3601 ggctaaaggg gttttcaact catgtgatgc tccacggaca aaggaaacct tttgattttg
     3661 caattttaca atttgctcat tacgactttc caactcatga ataaccttca acaagtgctc
     3721 atacatacca ttaatctgtt ttccaacttc accaacttca tctttcctac tttcatcaaa
     3781 acgaatattg tcatccaaat cttgcatttt actagtcact tctgaaatgt aaaaaagagg
     3841 attgagcaag cgtttagtat aaaaataaga aaaaacaaag gaaaaagcaa ttgtaaccaa
     3901 aaatgtatat gggagataca aaagcaaaat attctttgct tctttgtaga catccacccc
     3961 gtgaacaaat tgcactgtta cctgtttacc atctgctgta ctaacagaca tatctaacat
     4021 cacgatataa tttgatagct ttccatcttt aatatccaag tcatgcacaa gagggagccg
     4081 cttatctaca atatctcttt taaggtgagc agatatggtc aaggactttg aataagactg
     4141 gagtaagctt ggtatttcat cagatgtctt agtctttaaa tactccgtaa acactcttgc
     4201 gctctcatta aacttttccc cctccagttg aatataataa aaaggaaagg tcaaataaat
     4261 agctatatga atgactagac caacaccacc tagaattgca aaacttctta aaaaagactt
     4321 ggttaataat ttaggatttt ttatcataag ctaatcttat acccaacatt tttcacagtc
     4381 acgatacaat ctaaagctaa ttttttgcga agagtacgaa tatagacatc aacgacccta
     4441 tcacaaggta aatcagctac atcttttgaa atttcttcca atatctgctc tctacttaaa
     4501 acttgatttt tatgctgaat caaacacttt agtaccagta attcctttgg tttgagatca
     4561 atttcttcat ttttataatg tgctttgtaa gaggtaaaat ccactgttac atcctgatat
     4621 cgccaaatat cctctatgac gtagtaacgt ctgataagcg ccttaatacg cttgacaagc
     4681 agtaccaaag aaaaaggttt tgtcacatga tcatcagcat agagatcaaa tacctgtgac
     4741 tgagtagttt catctcccag agcactcaag acgatgacag gaatatcgtg cttttgattg
     4801 atttctttta acacttcaaa accactcttc ctaggcatca ttaaatccag taccatgaga
     4861 tggataggga gatcttgaaa tttttccaag acctcttgtc catccttagc cataatgaca
     4921 tgataaccct cttctgtcag aaatgctgca attccttctc taatcatttc ctcgtcatct
     4981 gcaactaaaa tgttcatctc tctccctttc tactaccaga actccataca tctatttcct
     5041 gtatgctatt tgaaaatttc ctaccagcct atcatctatg ataatggaat ggatttcatt
     5101 tatccgctac tttttgtcaa caacaacgtc aatacaagaa aatcattcat cttgcagctt
     5161 atactcttcg aaaatctctc caaaccacgt cagctctatc tacaacctca aaacagtgtt
     5221 ttgagcaacc tgcggctagc tgcctagttt gttctttgat tttcattgag tattagtttg
     5281 atttttcaaa taccagttag aacggatttg acatgcaaaa aagaaacagg tctcttttga
     5341 aagaataaaa atttcagata agactgctct ttttctacta cacacgctct aagacaaata
     5401 cgacggaggg taataaacat atgaattttg tgttgaatgg tgaaaaccac gaatcgttta
     5461 gaagctatag gttttagttt catactcttc gaaaatctct tcaaaccacg tcagctctat
     5521 ctgcaacctc aaaacagtgt tttaagcaac ctgcggctag ctgcctagtt tgctctttga
     5581 ttttcattga gtttcactcc actgtgactt atggtcagat gctgatactt ttattctttt
     5641 atgcaatatc aaattttaac tggcctgctt tgacaccaat cttaagtgtg ctgcctgcca
     5701 ctaaatctcc cttaagaaga agttctgcca acttgtcctc cacttctgtt tgcagggttc
     5761 tgcgaagtgg gcgagctccc atctctgggt catatccttg atttgctaac aatttcagag
     5821 ctgaagcttg taatttcaag tcaatgcctt tttcagccaa acttgccact aaaggcttga
     5881 ccataatctt caccacttcc tgcatatggt cactagatag gctatggaag accaccttct
     5941 catcaatacg gttgatgaat tccggtctat aagctttttt cagttcttca aacatgcgtt
     6001 tttccatatt ttcctggtca aaacgaatat ccttagcccc aaaaccaaca gtcttatcat
     6061 cacgaagggc agtcgcacct agattcgatg tcataatgat aatggtattt gaaaaatcga
     6121 ccttgcgtcc cttgctatct gtcaagacac cgtcatccag aacctgcaag agaacattaa
     6181 agatatctgg gtgagccttc tctacctcat caaagaggag aacggaatag ggtttattgc
     6241 gaaccttctc tgtcaactcc ccaccttctt cgtaacccac atagcctgga ggagctccat
     6301 ttagacggct ggctgcgaat ttctccatat actcactcat atcaaagcgg ataagggctg
     6361 attcgtcatc aaagagaact tctgccaaag ccttggccaa ctcggtctta ccgacacctg
     6421 tgggtcctag gaacataaag gaaccgatcg gacgcttgtg actgcgaatt cctgactgat
     6481 tacggcgaat cgcacgacta atacttgaaa cagcttgatc ttgaccgatg acacgtttat
     6541 ggagttctgc ttcaagattt aaatacttct tagcatccgt ttgagtcagt ttttgaactg
     6601 ggattcctga caagcgactc aaggtggtca aaatatcaga ctctgtcacc aagtctttgt
     6661 agacaggtac ttcctcttct tttgcgatta gctgggctgc ctgtttccac ttgccatcca
     6721 tcagggcctt gtcagctgga ctcaaatctg aatcgtctgc ttttacatgc tttgccttat
     6781 tttgcactgt tgctgctgct tcgtccaaga ggtcgatagc agagtctggc aagtgacgac
     6841 tggttaaata acgatgagcc atcttaaccg ctgtttcaac cgcttcatct gtgatttgta
     6901 cacggtgatg tttctcataa gtcgccttca aaccttgtaa aatagtcata ctatctgcca
     6961 cacttggttc ttcaatcgtc actttagcga aacgacgaga aagagccgca tctttttcga
     7021 tatgtttttg atattcttcc tgagtggtgg caccaaccgt tctcaaagtt ccacgcgcca
     7081 aggctggttt caagatattg gccgcatcca gagtcgaatc aatcccgcta ccagaaccca
     7141 tgatggtgtg gagttcatcg ataaagagga tgacttggcc atcttcttca atatccttga
     7201 tgatattatt catgcgttct tcaaagtcac cacggaagcg tgtccctgca acgacattca
     7261 tcaaatcaag ttctaacacg cgcatcttag ccatttccgc aggcacgtca ccactagcaa
     7321 tacgctgagc aagaccaagg gccagagctg ttttcccgac accagcatcc ccaaccaaga
     7381 cagggttgtt cttagtctta cggctcaata tttgaatcat acgtgagatt tccttgtccc
     7441 gaccgatgac tggttctaac ttgccagaac gcgcttgctc tgtcaaatca tgcgtatagt
     7501 cctcgagacc accactagga gtctgcggca tgcccatcat attggccata gaattttgct
     7561 tgtcagctac tgtacgatgg cgttggcgta aagccttgag atcttcacga gtccagcctg
     7621 cacgttcttc taaatttcga cgaagggcag caatcttgac ctgatctttc ttgtcttcat
     7681 aagaaaaacc agccctctcc aagatacgag tcgccaaggc attgccatca tgcaaaatcg
     7741 catagaggac gtgctctgtc cctagtacct tagcatggac cactgacgct acatactctg
     7801 cttcatcaaa aagaacctgc aaacgacggg agaacggcaa ttccgtaaag gtttcatcct
     7861 ggctatagtc cgtttcagtc agttccaaag ccacctcttc taaacggtcc atctcatacg
     7921 gataatcatt taaagttgcc cctgctacac tataactgtg attagacatg gcaatcaaca
     7981 agtgccacga ctctagataa cgagctccaa aatgtccagc aaccatgtag gcactttcga
     8041 tacattcatt caatgctttt gaatagttca tcttacttcc cttttctatc tacctcttgt
     8101 atgacctgac gtagcatgtt tgctcgaaca actggagctt cttctcctaa aacgcgatcc
     8161 aaagctactg attctagcaa attcatctcc tgcttggtca tcaattcctg ctcaaccaaa
     8221 agctggagaa tatcctcata aatttcttga ctgactcgct caccaatcga gtaaagcaga
     8281 tcccggagca tttcatgatg actagaaaac tcaatccgtc ctatacgaat gtagcctccg
     8341 ccaccacgct tactttcaac caagtagcct ctactttccg taaagcgtgt cttgatcacg
     8401 tagttaatct gactaggaac aacctgaaag gtatctgcca actgactccg ttgcaactcc
     8461 acgataccag attgatctaa aatcgccttg atgtaggcct caatatgatc cgatgtattt
     8521 ttaaatctca taacaaaccc acctctctct ttaaaccttg accttgtttg actattatac
     8581 cgaaattttc tcatttttaa aagaaaaagg gcgctggtaa aggataatct tcaccaactc
     8641 cctatttttc tacttatcta agcctaattc tgccaagatt tgacgtttgt aggcaatctt
     8701 ttggacttcc ttgtcctcat cttcagacca atctagttta atttctgaaa caatctgccc
     8761 agggcgattt ttcaagatat agatgcggtc gctgagattg agggcctcct caatactatg
     8821 cgtgatgatc agagttgtta gctgcaactg tttgtgaatc tcaagatacc aagcgtggag
     8881 ttccatcttt gtcatctcat ccaaggcgct aaaggcctca tctaagagaa agagcttgtg
     8941 cccaaaaagg taggtccgga gtaaggctac acgctggcgc atcccaccgc taagttcatg
     9001 aggatacttg tctcttacag ctgtcagctg gaaggtcgca agaattttat ccgctcggga
     9061 aatagcttct gccttatcca ccttttgaat caagaggggc agaatgatat ttccaagcac
     9121 cgtcttgtgc tccaagagca gatccttttg caacatataa ctcacgcgcc ccttgggatt
     9181 ttcttcacca tcaaggacaa ttctccctga ctgaacttct aaaatcccag cgattagatt
     9241 aaagagggtg gtctttccaa caccacttgg gcctaggatg gaaaccactt cgcctgaagt
     9301 cacctgtagg ttgatatcct ctaaaatcct ctcctgacca taggcataac tgacgtgctc
     9361 tagtctaatt tctgtcatta tttcacaaat tcgttggtga agcctttgtc tgtcaagtct
     9421 tctttaagga taccattttc tttatcccat ttgtagaaag cattccagcg agctgcgtca
     9481 aattgacccc atttttcctt gtcgcttgcg tattcttttg acaagtattt ttgagattcg
     9541 atgacaaagt cacgtttttc cttgagttca ggtgcattct tgatgagaat atctgcagct
     9601 tcttctggat gttccatggc atattggtag ccttttttga tggcttggat gactttgcga
     9661 gcttcttctt tgttatcttt cagatagtcg ttgtttgcga tgataactgg tgaatagtag
     9721 tcaaactcct tgacatagtc tttcaagtac atgaagttag catctacacc ttgagattta
     9781 gcaaggatac catcccaacc gtagtaaatc caagcagtat caaagacgcc attggcaatc
     9841 ggtgtgattg agtttgagtc gttatttggt actttttcaa ccttctcaaa gtctccacct
     9901 tgagattcta ccaaggtttt caacatagca agttcagttg ggtcattcca tgtcccatat
     9961 ttcttaccaa ccaagtcttt tggactgctt acattatcag atttacgaga gatgattcct
    10021 gatgtattgt gttcaacaat agctgcaacg gcagtgattc ctgctccttt ttccaatttc
    10081 ttagccatgt agtcttggaa atacactgca aatggtgcct ttccgttgat aaccaagtca
    10141 gaagaacttt cttctggtgg caatttcaaa tcaacatcca ctccagcttc tttgaaataa
    10201 cctttttcct tggcaacata aagccctgtg tggttggtat ttggtgtcca gtctaggata
    10261 aagtcaacct tcttaagttc tgcctctttg ttgtctttag aagcagttcc ttgaccacag
    10321 gccacaagca caacagctac aagagctgtt acaagcgtta aaaacacttt ccatgttttc
    10381 ttcattttga tttcctcttt tctttttcag aaacattcta attctacgaa cgtttccatt
    10441 taatcacata tttttcactg atatcgacca gcttcatacc caaaagactg ataatcgaca
    10501 ccagaataat aatggcaaac atggtatcat actgaaacaa ttttttagac tgaatcatat
    10561 aaacaccaag accttcaaaa cctcccaacc actcagatac cacagttgtg ataaaggcgt
    10621 aggagacact gaccctcaga cctgcataaa agtaaggcag gctaactggg attttaaaat
    10681 gccacaggat ttgccaaggc ttggcccgca tcagactaaa caaggtcagc atatccttgt
    10741 cgcaatgcct aaaaccgtcc aaaatactaa cgatgatggg aaaggttgtc gttaagataa
    10801 tcaagacaat cttgggcaaa atcccataac ctagccacaa gaccaggata ggagctatgg
    10861 caatggtcgg aatggtctga atgaccacca tcatagggta aatcaggtca ttgagccaag
    10921 tcaaactatc catgagcaca gccataagac aggcaatcaa aactcccaaa atcagcccca
    10981 gtaaagccac tctcaaggtc gcccagctat ggtgccagag aaattctctg tcacgaacaa
    11041 agggctggag aatttcaaga ggtgtcggca ggataaactt ggggagaagt ttaagaaaac
    11101 ctgctaactg ccagattaac aagactccga gaaagcccaa tagactaatg tgtcgtctca
    11161 gtatacttct caagtttctc atcaatactt aaaatctctc ctacatttat tttgacattg
    11221 gcaaagacat tgtctgcctc ctgccctgcc acttccagcg cttcttttag aatgcgcata
    11281 agctcatcaa actccccttc caagaccgtt tcaaatggtg tcactaccat cgtcacttct
    11341 tgagtttgca gataagcaat gacctgatca ataacagcta tccgatcaat cccctgtgct
    11401 aggggtaaaa cttgcaaggc aatgcttgct ttcataagaa cctcctcttc tcgttttcct
    11461 ttttaaaaaa agaaaaacct ttaccataca tggaaaaggt gcaaaattag actaagtatc
    11521 gttccctccg ctggcattac ccagatcagg tgcggtcgaa gtttaacact tcctctcaga
    11581 ctgtacacag actcccatat attatatgta tatatgatag cgcaaaataa aaaaaatgtc
    11641 aaggaaactg cttacagaaa agagcaggaa atttctccta ctccaatttt ctatactcgt
    11701 tctccatcac tgtttactct gtagccatcc accgtcgtat taacagctaa ggcgcctgaa
    11761 gagtaagaat aataccactt accagagacc tggtaccagc ctgttttcat tgcccctgaa
    11821 ctacccagat aataccacga gccattctcc tttaaccatc ctgtctgcat ttcaccagat
    11881 aatttcaaat aataccagct agaaccatct ttcagccatc ctgttgattt agaaccattt
    11941 gatttgaaat gataccaact accatttatt ttcttccaac cgacagttga actattattg
    12001 gatccatagc tagagatatt tcctgattga ttctgataat tttgtgaaga agtcactccc
    12061 tctagctgta tatagcgacg acttccatta taggcagtat aactcaacca cttgtagccg
    12121 tctttttcaa gtatctgatc ataatgaacc ttctccccag gataatagta atcaatcaca
    12181 gttgcactaa ctaggggttc agttttaata gcagacttgg tcttaaaata atgagttcct
    12241 cctgttgaag aaagaacaga atgacctaga ggatttttat tcacagcctc caattgaaca
    12301 taacgataac ttccattata ggcagtataa ctcaaccact tatagccgtc tttttcaagt
    12361 atctgatcat aatgaacctt ctctccagga taatagtaat caatcacagt tccgctagct
    12421 agaggttcag ttttaatagc agacttggtc ttaaaataat gagttccgcc tgttgaagct
    12481 gaggattgac tattcccaac actgccacca tccaaatctt taaaatgaat aaatcctgtc
    12541 atcgtgtttg cttttacaac tcgtttatta taggattctg tataaccata gttatattcc
    12601 tcaatctcaa tctgatctcc cattacattt gacacccagg caacatgacc ataagttcct
    12661 gcagtagacc aagtaatgga accaatcgtc ggtgtattat ctacacgata accttcccga
    12721 cgagcacgat gtccccattc attcgcattt ccataagctg ccggcatttc aaaaccattg
    12781 acattactca aacgaaaggc tacaaaagaa gtacactgac gagaatacat gcgccactga
    12841 tcaatctcct ggctcccatt tttataataa gcaggataat catccccacg cgcaatcgat
    12901 ccatttcctc tggaataggc atagacacta tcaccagctg ctagcatcaa tcctactact
    12961 aaaaaaggaa caagcttttt aactgacatc ttcaagtaat aacttgttcc tcttgctata
    13021 aacggtaaaa ttttcattct tcctccttga aaaataatat aaagcgatga ttgccttcac
    13081 ctaaattatt cggagaaaaa agaaaaagtg agaaaatttc tcactttagt caagataatg
    13141 aatcaaattc ttttctgtaa gaatatctga gtaagtaaag gattcaacgt aaacattaac
    13201 ttgtttatct ccttccacat ccccaaactc cacaataaat agagatggat aaacttcaat
    13261 tagcttaccc aatctatttt tttggcgctt acgaccattc tccaaagtca tttctacgac
    13321 ttgtccctca tgtgccttga tttcttcttt gatttttttc atcttggcta catctgtaaa
    13381 tgcatctgac atcttatgcc tccctctttg agatacttga aaatttattg tattcttttt
    13441 ggaaaatcaa ttccactgtt ccacgagctc cactacggtt tttctcgata ataacttcca
    13501 ccttattatt tgggataccc tcctcttctt caccaccacg ttcatagtag tcatcgcgat
    13561 agagaaaagc tacgatatca gcgtcctgct caatagaccc agattcacga atatcagaca
    13621 agaccggtct cttgtcctga cgttgttcta caccacgaga aagctgactc agagcgatta
    13681 ctggaacctt caattccttg gctagtattt tcaactgacg agaaatttca gaaacttctt
    13741 gttgacgatt ttctcgacca gttcccgtga taagttgcaa atagtctatc aaaatcaaac
    13801 caagatttcc agtttcttga gccaatttac gagaacgaga acgaatctct gtaatccgaa
    13861 tacctggcgt atcatcgata tagatactgg cgttagctag attaccctga gcaatagtat
    13921 atttttgcca ctcctcatct gtcaattgcc ctgtacggat agaatgtgac tccactaagc
    13981 cttctgcagc taacatacga tctaccaagc tttccgcacc catttcgagt gaaaaaatag
    14041 caaccgtttt gtccaactta gtcccaatgt tctgagcgat attcaaggca aatgctgtct
    14101 taccaactgc tggacgagct gctaagataa tcaactcctc ctcatgaagt cctgttgtca
    14161 tatgatccaa atcacgataa cctgtcgcaa tacctgtaat atcggtcgtt tgttgcgagc
    14221 gagcttccag atttccaaag ttgagattca acacatctcg aatgttctta aacccgcttc
    14281 gatttgcatt ttcactgaca tcaatcaacc ctttttctgc ctgagcaata atttcatcag
    14341 ctggttgtga cgcttcgtaa gcttggttga cagactctgt caacttggca attaaacgac
    14401 gtagcattgc tttttctgca acaatcttag cataatactc cgcattagca gaagttggca
    14461 cagaattgac aatctcaacc aagtaagaca agccaccaat attctgcaaa tcaccttgat
    14521 tatcaaggat agtacgaacc gttgttgcat ctatggcatc accacgatcg gataaatcga
    14581 ccatggcttg gaaaatcaaa cgatgggcat acttaaaaaa gtcccgagac tcaatgtatt
    14641 ctcgcacaaa aacaagttta ctctcatcaa taaagatagc ccctaaaacg gattgctcag
    14701 ctaagatatc ttgaggttgt actcgtaact cttctacttc tgccatcaga cttcccttcc
    14761 ttttacaatc ttgtcaagaa agtgtaaact tatccttctt tcacacgaag attgattaca
    14821 cttgtgatat cttgatagat tttcactggc acatcaatca aaccaaccgc tcgaatcgga
    14881 gcttgtactt gaatatgacg tttatcaatc ttaattccaa attgcttttg caattcttct
    14941 gcaatcttct tattggtaat agaaccaaag gtacgaccat ctggaccaac tttttcaaca
    15001 aattctacaa cagtttcttc tgcttcaagt tgtgctttaa ttgcttttcc ttctgcaatc
    15061 atctcagcgt gagctttttc ttccgatttt tgtttaccac gaagttcacc tacagcttga
    15121 gcagtcgctt ctttggctag attctttttg ataagaaagt tttgcgcata ccctgttggt
    15181 acttccttaa tttcgccttt tttacctttt cctttaacat ctgctaaaaa gattactttc
    15241 attcttcttt ctccttttcc tttatttcat ttaatacaat ttctgtcagt ttttcacctg
    15301 cttctgacaa ggttacatct ttaatttgag ctgctgccaa attaaagtgg cctccaccgc
    15361 ctaactcttc cataatccgt tgtacattca gtttactacg acttcgagct gagatagaga
    15421 taaatccttg tgtattcttc gcaagaacaa aactcgcttc aatacctgac atggctaaca
    15481 tggcatctgc tgccttacta ataacaactg tatcatagca tttcgtgtcc ttagcctctg
    15541 ctattagtac atctgaacct aatttacgcc cctgtaaaat aagttcattg acctcacgat
    15601 attcttcaaa atctgtcgca gcgatttcct ggatagcaat actatcactt ccgcgcgttc
    15661 tgagatagct agcaacatca aatgtccgac tagttactcg cgaggtgaaa tttttagtat
    15721 ccaacatcat accagccatc aaaacacttg cttgcatacg actcaaacga tttttcttag
    15781 aattctggaa ctgaatcaat tccgttacca actcactggc actacttgca ccactttcga
    15841 tataagtaat gaccgcatta tctggaaaat cctgatccct tctatggtgg tcaataacaa
    15901 tggtttgggt aaataaatca taaaattctt ttgataatgt taaggctgtc tttgaatggt
    15961 ctacaagaat caacaaagaa cgattggtca ccatccccat tgcatcctta acagacaaca
    16021 acttcgtaac tccttctttt tctatgaatg aaacagctcg ttcaatatct ggagacattt
    16081 gttcttcatc ataaagagca tagctatttt caatcacatt gctggcgaac aactgcatac
    16141 ctacagcaga gcccaaagca tccatgtcta aacttttgtg accgactaca aaaacctgat
    16201 ctacactccg aatcttatct gaaatagctg tcatcatagc gcgcgtacga gtccgtgtac
    16261 gcttgattga agcagcagac ccaccaccaa aataaactgg atttttcgtt tcgttgtttt
    16321 ccttaacaac cacctggtcg ccaccacgta cttcagccaa gttcaaattg agcaaagcaa
    16381 ctttccctat ctcatcatga tttccatcgc cataagaaaa tcccatactt aaggtcaagg
    16441 gcaactgtct ctgtttcgac tcttctctga aagcatcaat aacagaaaat ttatcattca
    16501 tcaagccctc aagcaccgtg tagtcagtaa atagataaaa tcgatccata cttacccgac
    16561 gagaaaacat catgtatttt tctgaaaact ctgatataaa attagctaca aaactattga
    16621 tttgactaat atctgactca gaagttgcat cctccaaatc atcataatta tccacagaga
    16681 caatcccaat cactggtcga cttgttacca attcatctgt tatggcttgt tccctggata
    16741 catctacaaa atacaaaaca ccggaagaag catccatatg aacagcataa cgcttctcac
    16801 caagcttggc ataagtagac ggatttccta ctgaagcctt aataatcgtt tgaacagctt
    16861 ctaaatcaaa atcaccatct tccttggtca aaatcaattc agcataggga ttaaaccact
    16921 caacctctcc agaagataaa ttcaatttca taacacctac aggcatctgt tccaatagag
    16981 ctgtcaaact ttcttccgct tggtggttta catactgtat ctgttctaca tcactccttg
    17041 tataatgcac tctcagtttc ttaaataaaa aaacatagcc tcctacaaaa agaaacaaaa
    17101 ttaaaaccgt caacagatta ttattaacaa aaataatgaa agtggataag actccaaacg
    17161 caatcaatcc tactagaata ggaaaaattg gacttacata aaattttttc attcaaaacc
    17221 tcttggcacc cattatacca taatacccct caaaaagcga ctttttaaaa gtgtaatcag
    17281 taattctatc aattaaatta taagaaaaag gtagtttaca attcagtaaa cctaccttta
    17341 cacatattga aattaagatt ctttaacctc taacaaacca atttcgccat cctcacgacg
    17401 ataaatcaca ttggttgtct gatcttcaac atccacatag ataaagaaat catgccccaa
    17461 taaatccatt tgtagaattg cttcttccaa atccattggt tttaaatcaa tttgttttga
    17521 acgaacaact ttagactgga caatatttga atcttccacc aaagcatctg taaataattg
    17581 accagttgct accttatttt tatttttacg ctcgattttt gttttatttt tacgaatctg
    17641 acgttcaatt ttatcagtta caaggtcaat tgaaccatac atatcttgag atacatcttc
    17701 tgcgcggaga gtaatagatc caagcggaat cgttacttcc actttagccg ttttttcacg
    17761 ataaactttt aagttaattc gggcatccaa ctcttgttct ggttggaagt acttttcgat
    17821 cttttcgagt ttagaaacta cataatcacg aattgcttct gttacttcta ggttttcacc
    17881 acggatacta tatttaatca tatgagtacc ttctttctaa acatttttgt ttttatgatt
    17941 ttattataac gctttcattc tatttttgca aattttttcc tcatcttaca agggaaaatg
    18001 tttttacatc cttagcacca gcttcttcca acagtttctt aacacgattt atagttgctc
    18061 ctgtagtata gatatcatct ataagtagga tttttttagg aatagtgact ccacttttaa
    18121 taaagaaagg aagttctgtc cccaagcgct ctgaacgatt tttagaagaa ctggctctct
    18181 cttctctttt ctctaataaa tccagatact caaagcctgc tgcctctacc aagccctcaa
    18241 cctgattaaa tcctctatta gcatatctat caggacttag gggaattaca acaaattgat
    18301 actctttgta ctttttcaac tcctcactta aaaatgaagc gaaaactttt cttaacagga
    18361 agtctccatc aaacttatac cgactgaaaa aatccttcat agcttgattg taagtaaaaa
    18421 tcgctctatg actgacttca actccttctt tacaccaaag ttgacaatct tgacactttg
    18481 ttgacaactc tgttttcata caatttggac agttctcttc cccaattctt tcaaaagtag
    18541 aatcacagtc tgaacaaaga caagagtcat cattcctcag aagtaagaga ctactaaaag
    18601 ttaaaacagt cttcatagtc tgcccacata acaagcactt catagaccag cctccttatt
    18661 catcatctga atttccttaa tcgccttctt gattgaagca tttaacccat catggaagaa
    18721 aagcaaatct cctgtcggtc tatccatgct tcgtccaact cgtccaccaa tctgaatcaa
    18781 actagacttg gtaaacaaac gatgattggc ctctactacg aaaacatcca cacaagggaa
    18841 ggtaactcca cgctccaaga ttgtcgtact gataagtatt gtcagttctc catctcgaaa
    18901 agcttgtact tgctctaatc gatcctctgt tacagaagat acaaagccaa ttttctcatt
    18961 tggaaattgc tcctgtaaga tttctgctaa ctgctcccct ttcttaattt ctgaagcaaa
    19021 aatgagtaac ggataagctg tctttctctg cttctcaata taggacttta actttggtga
    19081 caaacgattc ttgtctaagt agcgattaaa atccgataac caaattggtt ttggaataat
    19141 caacggattt ccatgaaacc gtctcggtaa attcagtctt tttagttctc ctaaacggac
    19201 ctttttatct aactcattgg tcgaagtcgc tgttaaaaag attctcaatc cattctcctt
    19261 tacactattc ttgacagcat ggtaaagcgt gggattatca acataaggaa aagcatctac
    19321 ttcatccact atcagcaaat caaaagcttg ataaaacttc aataactgat gggttgttgc
    19381 aataactagt ggtgttcgaa aataaggttc cgattctcca tgtagcaaag ctatcccgca
    19441 agaaaaatcc tgttgcaggc gcttgtacag ctccaaacaa acatctatgc gaggactagc
    19501 caaacacact gcaccacccg cattgatcac tttagccact acttgataaa tcatttctgt
    19561 ctttccagct cctgttaccg catgaactaa ggttggcttt tgcttgtcta ctgcttgaag
    19621 cagtccctct gacaccttct cttgaaaagg agttaattgg ccgcgccatt tgagaacatc
    19681 ttgcttcgga aaatcctcct gcggaaaata gtataaagtt tgatcacttc tgactcgctt
    19741 catcagcaag cactctcgac aatagtaagc accgatgggc aaataccatt cttctagaat
    19801 agtgctatta caacgttgac agaaaagttt ccccttctcc tttctcattg ctggaagttt
    19861 ctccgccaac tgacgttctt cttctgttaa ttcattctca gtaaataaac gaccgagata
    19921 atctaaattt actttcatac ttctttattc gtaaaaacta gcactttaga tgatttttta
    19981 gtacaattaa atcatggaat ttaggacaat taaagaggac ggccaagtcc aagaagaaat
    20041 caaaaaatct cgctttatct gccatgccaa gcgtgtttat agcgaagaag aggctcgtga
    20101 cttcattact gccatcaaaa aagaacacta caaagcgaca cataactgct ctgcctttat
    20161 tattggagaa cgtagtgaaa ttaaacgtac aagtgatgat ggtgagccta gtggtactgc
    20221 tggtgttccc atgcttgggg tactagaaaa tcacaatctc accaatgtct gtgtggtcgt
    20281 gacacgctac tttggtggta ttaaactagg cgctggagga ctaattcgtg cttacgccgg
    20341 cagtgtcgcc ttagctgtca aagaaattgg tattattgaa ataaaagaac aggctggcat
    20401 tgctattcaa atgtcttatg ctcagtacca agagtacagt aacttcctta aagaacatga
    20461 tctcatggag ctggatacaa actttacaga tcaagtcgat acgatgattt atgttgataa
    20521 agaagaaaaa gaaactatta aagctgcact tgtggagttt tttaatggaa aagtcacttt
    20581 aactgaccaa ggtttacgag aggttgaagt tcctgtaaac ttagtgtaaa caatgaataa
    20641 tacagcgttt cgttgacatt ctcacaacta ctttagcgag caaaataaaa agatgcgtac
    20701 caaaatatac tagaaaatga agcaattcaa acgaaacctg atatcgtttt ccttcacacc
    20761 tatttactag aattaactga acgcaatcac ttgaaaatta atgactttga tctatgatat
    20821 atagaaatcc tatcgcttcg ataggatttc tatattttac aaatggtatg gatagcgtta
    20881 tactaaagat atcttataca aagaggtatt catatgtcta tttataacaa cattactgaa
    20941 ttaatcggtc aaacaccgat tgttaaactt aacaacatcg tgccagaagg tgctgcagac
    21001 gtctatataa agcttgaagc atttaatcct ggttcatctg taaaagaccg tattgccctt
    21061 agcatgattg aaaaagctga acaagatggt attctgaaac ttggttctac tattgttgaa
    21121 gcaacaagtg gaaacaccgg tattggactt tcatgggtag gtgctgctaa agggtataaa
    21181 gtcgtcatcg ttatgcctga aactatgagt gtagaacgac gtaaaattat ccaagcttat
    21241 ggtgctgaac tcgtcctaac tcctggtagc gagggaatga aaggtgctat tgctaaggct
    21301 caagaaatcg ctgctgaacg tgatggtttc cttcctcttc aatttgacaa tccagctaat
    21361 ccagaagtac acgaaagaac aacaggagct gagatactag ctgctttcgg taaagatgga
    21421 ttagatgcct ttgttgctgg agtaggtact ggtggaacga tttctggtgt ttctcatgca
    21481 ctcaaatcag aaaattctaa cattcaagtt tttgcagtag aagcagatga atctgctatt
    21541 ctatctggtg aaaaacctgg tcctcacaaa attcaaggta tctcagctgg atttattcct
    21601 gatacacttg atactaaagc ctatgatggt atcgttcgtg taacatcaga tgacgctctt
    21661 gcactcggac gtgaaattgg tggaaaagaa ggcttccttg tagggatttc ctcagctgca
    21721 gctatctacg gagccatcga ggttgccaaa aaattaggta caggtaaaaa agtccttgcc
    21781 ctagcaccag ataacggtga acgttatctc tctacagcac tttatgaatt gtaaccgtcc
    21841 aataacgaag tctattgaaa aatctccaga ctagagaact cacggatagt tcctaatctg
    21901 gagatttctt atttgcactt ttcttgtaca actttagtcc atggtaaata ggcctctaaa
    21961 acctctttgt ttacgagagt ttccacgttt agaagacatt ctagaagata ggatagatat
    22021 ttctcactat ttataatgga ttgaaataag atatgaacaa atcgattacg acatgatggt
    22081 aaagcgtaat cccttgtttc tcagctttcc cagacaaaaa agtccaatag taagtcagct
    22141 gactatcact ctctagcacc ctataagaag tttcatccgc atgaagtaag ggctgagtca
    22201 atagtctctc tcgcaagagg ttataaaggg gctccaaata gtattgactc gtcttgatat
    22261 gccgattaga gatttcctta cgtgtgattg gtaaacccat cctagcccaa tcttcttctt
    22321 ggcgataatt gggtaccttc agattaaact tctgatggat ggtgtgagcg ataatagaag
    22381 ctgagccaaa gttattcgct aaaggggctt taggaatagg agatttcaca agcttatcca
    22441 gatgattatc ttttactcgt tatggacaat gctatatggc ataaatcaag taccttaaag
    22501 attccgacta atattggctt tgcatttatt cctccataca caccagagat gaaccccatt
    22561 gaacaagtgt ggaaagagat tcgtaaacgt ggatttaaga ataaagcctt tcaaattttg
    22621 gaagatgtca tgaatcaact ccaagatgtt atacaaggac tggagaagga ggtgataaag
    22681 tccatcgtta atcggagatg gactagaatg ctttgtgaaa acagatgagt ataaaaagaa
    22741 agtcctcatt tcaatagaaa tcacgacttt ctgatgaatt tatagtaaaa tgaaataaga
    22801 acaggacagt caaatcgatt tctaacaatg ttttagaagc agaggtgtac tattctagtt
    22861 tcaaaccact atatttgggg agtgatagaa aagcccttca taagccagtc tacttgttca
    22921 ggtgcgagag ctttgacatc cttttctgta cttagccaag tcagttttcc gttctcaaag
    22981 cgtttatata gtagccaaaa tccttgacca tcccagtaaa gggctttaaa gcggtcttta
    23041 c
//
LOCUS       DAVKWZ010000032        22166 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000032 DAVKWZ010000000
VERSION     DAVKWZ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22166
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.032"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            1..267
                     /locus_tag="VW854_001268"
     CDS             1..267
                     /locus_tag="VW854_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000962022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGC"
                     /protein_id="HEV0262502.1"
                     /translation="MLVVLLIISVLFLLFVPNLTKQKEAVNDKGKAAVVKVVESQAEL
                     YSLEKNEDASLRKLQADGRITEEQAKAYKEYHDKNGGANRKVND"
     gene            236..664
                     /locus_tag="VW854_001269"
     CDS             236..664
                     /locus_tag="VW854_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEV0262503.1"
                     /translation="MEEQIVKSMIKAFTMLESLLVLGLVSILALGLSGSVQSTFSAVE
                     EQIFFMEFEELYRETQKRSVASQQKTSLNLDGQMISNGSQKLTVPKGIQAPSGQSITF
                     DRAGGNSSLAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            669..929
                     /locus_tag="VW854_001270"
     CDS             669..929
                     /locus_tag="VW854_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262504.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            892..1353
                     /locus_tag="VW854_001271"
     CDS             892..1353
                     /locus_tag="VW854_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEV0262505.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEVELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTNARGRGYQPMVYGLKSVRITEDNQLVRFHFQFQKGLEREFIYRVEKEKS"
     gene            1331..1744
                     /locus_tag="VW854_001272"
     CDS             1331..1744
                     /locus_tag="VW854_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEV0262506.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQVAHYQDYALNK
                     EKLVAFAMAKRTKDKVEQESGEQFFNLGQVSYQNKKTGLVTRVRTDKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKVEKKKSEEKPEKKENS"
     gene            1881..2475
                     /locus_tag="VW854_001273"
                     /pseudo
     CDS             1881..2475
                     /locus_tag="VW854_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            2536..3489
                     /locus_tag="VW854_001274"
     CDS             2536..3489
                     /locus_tag="VW854_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV0262507.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLTSLDKKVDYLGMEMDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSNLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKNEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            3540..4730
                     /locus_tag="VW854_001275"
     CDS             3540..4730
                     /locus_tag="VW854_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEV0262508.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGADAIVFTAGVGE
                     NAESFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            4879..5250
                     /gene="rnpA"
                     /locus_tag="VW854_001276"
     CDS             4879..5250
                     /gene="rnpA"
                     /locus_tag="VW854_001276"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEV0262509.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            5225..6049
                     /locus_tag="VW854_001277"
     CDS             5225..6049
                     /locus_tag="VW854_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV0262510.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            6068..7054
                     /locus_tag="VW854_001278"
     CDS             6068..7054
                     /locus_tag="VW854_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEV0262511.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQTIIDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNCYSRTFYITINVNDYVEHRAEVLQTYAQKL
                     AIRVLEEGRSHQTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            7105..7728
                     /locus_tag="VW854_001279"
     CDS             7105..7728
                     /locus_tag="VW854_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEV0262512.1"
                     /translation="MLNKIRDYLDFSGLQYRSPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQTKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            7859..9316
                     /locus_tag="VW854_001280"
     CDS             7859..9316
                     /locus_tag="VW854_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV0262513.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSFMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFIFGLIGQLITIVLLIVFKNPILIIIGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            9339..9620
                     /locus_tag="VW854_001281"
                     /pseudo
     CDS             9339..9620
                     /locus_tag="VW854_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
     gene            9697..10182
                     /locus_tag="VW854_001282"
     CDS             9697..10182
                     /locus_tag="VW854_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV0262514.1"
                     /translation="MNLKQALIDNDSIRLGLEANDWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            10199..10864
                     /locus_tag="VW854_001283"
     CDS             10199..10864
                     /locus_tag="VW854_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEV0262515.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAVDPAGAARAFKDEIKR
                     IWG"
     gene            10868..11731
                     /locus_tag="VW854_001284"
     CDS             10868..11731
                     /locus_tag="VW854_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEV0262516.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFIEMSIDERDERL
                     ARLDWSKEERLEVVKEIYETGVRIPSICFSGHRRYPLGSKDSVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKSCDWAEEAQVVLAIEIMDDPFIS
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            11733..12437
                     /locus_tag="VW854_001285"
     CDS             11733..12437
                     /locus_tag="VW854_001285"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEV0262517.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            12616..14289
                     /locus_tag="VW854_001286"
     CDS             12616..14289
                     /locus_tag="VW854_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV0262518.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALGDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            14401..15492
                     /gene="ulaG"
                     /locus_tag="VW854_001287"
     CDS             14401..15492
                     /gene="ulaG"
                     /locus_tag="VW854_001287"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEV0262519.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKNFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            15606..17582
                     /gene="tkt"
                     /locus_tag="VW854_001288"
     CDS             15606..17582
                     /gene="tkt"
                     /locus_tag="VW854_001288"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV0262520.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDANRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKIVRNLK"
     gene            17699..17998
                     /gene="yajC"
                     /locus_tag="VW854_001289"
     CDS             17699..17998
                     /gene="yajC"
                     /locus_tag="VW854_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV0262521.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            18045..18473
                     /locus_tag="VW854_001290"
     CDS             18045..18473
                     /locus_tag="VW854_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEV0262522.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNVSDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            18475..18885
                     /locus_tag="VW854_001291"
     CDS             18475..18885
                     /locus_tag="VW854_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262523.1"
                     /translation="MENLTNFYEKYRVYLTRPRLEILAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            19185..21836
                     /gene="adhE"
                     /locus_tag="VW854_001292"
                     /gene_synonym="adhC"
     CDS             19185..21836
                     /gene="adhE"
                     /locus_tag="VW854_001292"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEV0262524.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRKLAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT         6983 a         3976 c         4840 g         6367 t
ORIGIN      
        1 atgttggtgg tcttgctgat tatcagcgtg cttttcttgc tctttgtacc taatctgacc
       61 aagcaaaaag aagcagtcaa tgacaaagga aaagcagctg ttgttaaggt ggtggaaagc
      121 caggcagaac tttatagctt agaaaagaat gaagatgcta gcctaagaaa gttacaagca
      181 gatggacgca tcacggaaga acaggctaaa gcttataaag aataccatga taaaaatgga
      241 ggagcaaatc gtaaagtcaa tgattaaggc ctttaccatg ctggaaagtc tcttggtttt
      301 gggacttgtg agtatccttg ccttgggctt gtccggctct gtccagtcca ctttttcagc
      361 ggtagaggaa cagattttct ttatggagtt tgaagaactc tatcgggaaa cccaaaaacg
      421 cagtgtagct agtcaacaaa agactagtct gaacttggat gggcagatga ttagcaatgg
      481 cagtcaaaag ttgacagttc ctaaaggaat tcaggcacca tcaggccaaa gtattacatt
      541 tgaccgagct gggggcaatt cgtccctggc taaggttgaa tttcagacca gtaaaggagc
      601 gattcgctat caattatatc taggaaatgg aaaaattaaa cgcattaagg aaacaaaaaa
      661 ttagggcagt gattttactg gaagcagtag tcgctctagc tatctttgcc agcattgcga
      721 ccctcctttt gggacaaatt caaaaaaata ggcaagagga agcaaaaatc ttgcaaaagg
      781 aagaagtctt gagggtagct aagatggccc tgcagacggg gcaaaatcag gtaagcatca
      841 acggagttga gattcaggta ttttctagtg aaaaaggatt ggaggtctac catggttcag
      901 aacagttgtt ggcaatcaaa gagccataag gtcaaggctt ttaccttgtt agaatccctg
      961 cttgccctca ttgtcatcag tgggggatta ctcctttttc aagctatgag tcagctcctc
     1021 atttcagaag ttcgctacca gcaacaaagc gagcaaaagg agtggctctt gtttgtggac
     1081 caacttgagg tagaattaga ccgttcgcag ttcgaaaaag tagaaggcaa tcgcctatac
     1141 atgaagcaag atggcaagga catcgccatc ggtaagtcaa agtcagatga tttccgtaaa
     1201 acgaatgctc gtggtcgagg ttatcagcct atggtttatg gactcaaatc tgtacggatt
     1261 acagaggaca atcaactggt tcgctttcat ttccagttcc aaaaaggctt agaaagggag
     1321 ttcatctatc gtgtggaaaa agaaaaaagt taaggcaggt gttctcctct acgcagtcac
     1381 catagcagcc atctttagtc ttttgttgca attttatttg aaccgacaag tcgcccacta
     1441 tcaagactat gctttgaata aagaaaaatt ggttgctttt gctatggcta aacgaaccaa
     1501 agataaggtt gagcaagaaa gtggggaaca gttttttaat ctaggtcagg taagctatca
     1561 aaacaagaaa actggcttag tgacgagggt tcgtacggat aagagccaat atgagtttct
     1621 gtttccttca gtcaaaatca aagaagagaa aagagataaa aaggaagagg tagcgaccga
     1681 ttcaagcgaa aaagtggaga agaaaaaatc agaagagaag cctgaaaaga aagagaattc
     1741 atagtcaatt caactataat gcgttgaatc cagaatagtc cactgtagtt tctagaaaat
     1801 tgctggaaat ggatgttaag ctccaattca tttgtttata tcttatttca gtttactata
     1861 ctttgtgcta aattaaagat atgaaacatg attttaacca caaagcagaa actttcgatt
     1921 cccctaaaaa tatcttcctc gcaaacttgg tatgtcaagc agccgagaaa cagattgatc
     1981 ttctatcaga caaagaaatt ttagatttcg gtggtggcac gggtctatta gccttgcccc
     2041 taacccctag ccaagcaggc taagtcagtc actcttgtag acatttctga gaaaatgttg
     2101 gagcaagctc gtttgaaagt ggagcagcaa gcaatcaaga atatccagtt tttggagcaa
     2161 gatttaccga aaaatccctt ggagaaagag tttgattgcc ttgctgttag tcgggttctt
     2221 catcatatgc ctgatttgga tgcggctctc tcactgtttc atcaacattt gaaggaagat
     2281 gggaaactca tcattgctga ttttaccaag acagaagcta atcatcatgg atttgattta
     2341 gctgaactgg aaaacaagct aattgagcat ggtttttcat ctgtgaatag tcagattctc
     2401 tatagcgctg aagacctgtt tcaaggaaat cactcagaat tctttttaac agtagcccaa
     2461 aaatcactcg cctagtcagg gagtgatttt tctataagga tggaaaaaag aagggaaatt
     2521 tgataagata ggaatatgga ttttgaaaaa attgaacaag cttataccta tttactagag
     2581 aatgtccaag ttatccaaag tgatttggcg accaactttt atgacgcctt ggtggagcaa
     2641 aacagcatct atctggatgg tgaaactgag ctaaaccagg tcaaagacaa caatcaggcc
     2701 cttaagcgtt tagcactacg caaagaagaa tggctcaaga cctaccagtt tctcttgatg
     2761 aaggctgggc aaacagaacc cttgcaggcc aatcaccagt ttacaccaga tgccattgct
     2821 ttgcttttgg tgtttattgt ggaagagttg tttaaagagg aggaaattac tatcctcgaa
     2881 atgggttctg ggatgggaat tctaggcgct actttcttga cctcgcttga taaaaaggtg
     2941 gattacttgg gaatggaaat ggatgatttg ctgattgatc tggcagctag catggcagat
     3001 gtaattggtt tgcaggctgg ctttgtccaa ggagatgccg ttcgtccaca aatgctcaaa
     3061 gaaagcgatg tggtcatcag tgacttgcct gttggctatt atcctgatga tgccgttgcg
     3121 tcgcgccatc aagttgcttc tagccaagaa catacttacg cccatcactt gctcatggaa
     3181 caagggctta agtacctcaa gtcagacgga tacgctattt ttctagctcc gagtaatttg
     3241 ttgaccagtc ctcaaagtga tttgttaaaa gaatggctga aagaagaggc gagtctggtt
     3301 gctatgatta gtctgcctga aaatctcttt gctaatgcca aacaatctaa gactattttt
     3361 atcttacaga agaaaaatga aatagcagta gagccttttg tttatccact tgctagcttg
     3421 caagatgcaa gtgttttaat gaaatttaaa gaaaattttc aaaaatggac tcaaggtact
     3481 gaaatataaa atagattttg ttataatagt tgaaaacgct taaaaagggg tatcatgtta
     3541 tgacaaaaac aattgcaatc aatgcaggaa gttcaagttt gaaatggcaa ttatacttaa
     3601 tgccagagga aaaagtgtta gccaaaggtt tgattgaacg tatcggtttg aaagattcaa
     3661 tttcaactgt aaaatttgac ggccgttctg aacaacaaat tttggatatt gaaaatcata
     3721 cacaagccgt taaaatttta ttggatgact tgattcgttt cgatattatc aaggcttatg
     3781 acgagattac aggtgttgga catcgtgttg ttgctggtgg agaatatttc aaagaatcaa
     3841 cagttgttga gggagatgtt ttagaaaaag ttgaagagtt gagtttgttg gctcctctac
     3901 acaacccggc caatgcagca ggtgttcgtg ccttcaagga attgttgcca gacattacca
     3961 gtgtagttgt ttttgatact tccttccaca caagtatgcc agagaaagct tatcgctacc
     4021 ctctaccaac aaaatattac acagaaaaca aggttcgtaa atacggtgct catggtacaa
     4081 gtcaccagtt tgtagcagga gaagctgcaa aactcttggg acgtccatta gaagacttga
     4141 agttaattac ctgtcatatt ggtaacggag gctcaattac agctgtgaaa gccggcaaat
     4201 ctgtagacac ttctatgggg ttcactcctc ttggtggtat tatgatggga acgcgtacag
     4261 gggatattga tccagctatc attccttatt taatgcaata tacagaggat tttaacacac
     4321 cagaagatat cagtcgtgtt cttaaccgtg aatcaggtct tttgggagtt tctgctaatt
     4381 ctagcgatat gcgcgatata gaagcagctg tagcagaagg gaatcacgag gctagcttgg
     4441 cttatgaaat gtatgttgac cgtatccaaa aacatatcgg tcagtacctt gcagtgctaa
     4501 atggagcaga tgccattgtt ttcacagcag gtgtcggtga aaatgcagag agtttccgtc
     4561 gtgatgtaat ctcagggatt tcgtggtttg gttgtgatgt tgatgatgaa aagaatgtct
     4621 ttggcgttac aggagacatc tcaacagagg cagctaaaat ccgtgtcttg gttattccaa
     4681 cagatgaaga attagtcatt gcccgtgacg ttgaacgctt gaaaaaataa gtgaaactaa
     4741 aaaaatattc aatacaagga gttgggaaag ttatttttcc agcttctttt tctgatgaaa
     4801 ttgtccaaaa ccttgctatg attggctttt ttgaaaaata tggtataata gtagtaattt
     4861 aatagatgga gttgagtttt gaagaaaaac tttcgtgtaa aaagagagaa agattttaag
     4921 gcgattttca aggaggggac aagttttgct aatcgcaaat ttgtggtcta ccaattagaa
     4981 aaccagaaaa accattttcg agtaggtcta tcagttagca aaaaactggg gaatgccgtc
     5041 actagaaatc aaattaagcg acggattcgg catattatcc agaatgcaaa agggagtctg
     5101 gtagaagatg tcgactttgt tgtcattgct cgaaaaggag tcgaaacctt gggatacgca
     5161 gagatggaga aaaatctact ccatgtatta aaattatcaa agatttaccg ggaaggaaat
     5221 gggagtgaaa aagaaactaa agttgactag tttgctagga ctgtctctgt taatcatgac
     5281 agcctgtgcg actaatgggg taactagcga tattacagcc gaatcggctg atttttggag
     5341 taaattggtt tacttctttg cggaaatcat tcgcttttta tcgtttgata ttagtatcgg
     5401 agtggggatt attctcttta cggtcttgat tcgtacagtc ctcttgccag tctttcaggt
     5461 gcaaatggtg gcttctagga aaatgcagga agctcagcca cgcattaagg cgcttcgaga
     5521 acaatatcca ggtcgagata tggaaagcag aaccaaacta gagcaggaaa tgcgtaaagt
     5581 atttaaagaa atgggtgtca gacagtcaga ctctctttgg ccgattttga ttcagatgcc
     5641 ggttattttg gccctgttcc aagccctatc aagagttgac tttttaaaga caggtcattt
     5701 cttatggatt aaccttggta gtgtggatac aacccttgtt cttccgattt tagcagcagt
     5761 attcaccttt ttaagtactt ggttgtccaa caaagctttg tctgaacgaa atggcgctac
     5821 gactgcgatg atgtatggga ttccagtctt gatttttatc tttgcagttt atgcgccagg
     5881 tggagtcgcc ctatactgga cagtgtctaa tgcttatcaa gtcttgcaaa cctatttctt
     5941 gaataatcca ttcaagatta tcgcagagcg cgaggccgta gtacaggcac aaaaagattt
     6001 ggaaaataga aaaagaaaag ccaagaaaaa ggctcagaaa acgaaataaa taaggaggaa
     6061 tctggtaatg gtagtattta caggttcaac tgttgaagaa gcaatccaga aaggattgaa
     6121 agaattagat attccaagaa tgaaggctca tatcaaagtc atttctaggg agaaaaaagg
     6181 ctttcttggt ctatttggta aaaaaccagc ccaagtggat attgaagcga ttagtgaaac
     6241 gactgttgtc aaagcaaatc aacaggtagt aaaaggcgtt ccgaaaaaaa tcaatgattt
     6301 gaacgagcct gtgaagacgg ttagtgaaga aaccgttgac cttggtcatg tggttgatgc
     6361 tattaaaaaa atagaggaag aaggtcaagg tatttctgat gaagtcaagg ctgaaatctt
     6421 aaaacatgaa agacatgcca gcactatctt agaagaaact ggtcacattg agattttaaa
     6481 tgaacttcaa atcgaggaag cgatgaggga agaagcaggc gctgatgacc ttgaaactga
     6541 gcaagatcaa actgaaaatc aagacttgaa agagatgggc ttgaaggtcg agcaaagtta
     6601 tgatattgcc caggtggcta cggatgtgac tgcttatgtt caaacgatta ttgatgacat
     6661 ggatgttgag gctacacttt caaatgatta taaccgtcgt agcatcaatt tgcaaattga
     6721 tactaacgaa ccaggtcgta ttatcggcta ccatggtaaa gtcttgaagg ccttgcaact
     6781 gttggctcaa aattatcttt acaactgcta ttcaagaacc ttctatatca ctattaatgt
     6841 caatgattat gttgaacacc gtgcagaagt cttgcagacc tatgcgcaaa aattggcgat
     6901 tcgtgtttta gaagaagggc gcagtcatca aacagatcca atgtcgaata gtgaacgcaa
     6961 gattatccac cgtattattt cacgtatgga tggcgtgacc agttactctg aaggtgatga
     7021 gccaaatcgc tatgtcgttg tagatacaga ataagtaaaa tcagggttga cctgattttt
     7081 tgctagctag aggaggttaa actgatgttg aataagataa gagactatct agacttttct
     7141 ggtttgcagt accgtagtcc tgataaagcg ggagcagagc gagagaagat gctggcgttc
     7201 cgccacaaag gacaagaggc ccgaaaggct tttacagaac tggccaaagc ctttcaagca
     7261 agccatccag aatggcaact ccaacagact agtcagtgga tgaatcaggc ccagcgtttg
     7321 agaccacatt tttgggttta tctacagaga gacggacaag tgacagaacc tatgatggcc
     7381 ttacgtttgt atgggacatc tactgacttt ggaatttctt tggaagtcag tttcatcgaa
     7441 cgtaagaagg atgagcaaac actgggcaag cagaccaaag ttttagacat tccaaccgtt
     7501 aaagggattt attatctaac ctactctaat ggtcaaagtc aacggtggga ggcgaatgaa
     7561 gaaaagcgtc gtactttacg cgagaaggtg agaagtcaag aagttcgaaa agttttagtg
     7621 aaggtagatg ttcctatgac agaaaattcg tctgaagaag aaatcgtaga aggcttattg
     7681 aagtcttatt ctaaaattct tccctattat ctagctacga gaaaataaga taatttgtaa
     7741 aacatcataa atcatacagt ccaagagtga acagtccgct gtgtaattct tggtcttttt
     7801 gtttgcgctt tcgcattata taataaactt acaaaaacaa ttcaaaagga gaacaattat
     7861 ggaagtcgtt tcaagtgttc taaattggtt ttctagcaat attttgcaga atcccgcatt
     7921 tttcgtaggt ttattggtgt tgataggata tgcacttttg aaaaaacctg cccatgacgt
     7981 tttttcaggg tttgttaaag caacagtagg gtatatgttg cttaacgtgg gtgctggtgg
     8041 tttggttaca acctttcgtc caatcttagc agctcttaac tacaaattcc aaattggtgc
     8101 agcggttatc gacccttact ttggacttgc tgcagcaaac aacaaaattg cagcagagtt
     8161 tccagatttt gttggaactg caactacagc tctattgatt ggttttggaa taaatatctt
     8221 gctcgtagct cttcgaaaga ttacgaaggt aagaaccctc tttattactg gtcacatcat
     8281 ggtacaacaa gctgcaacag tatcttttat ggttctattc ttagtaccac aattgcgcaa
     8341 tgcttacggt acagcagcga ttggtatcat ctgtggactt tactgggcag ttagttcaaa
     8401 tatgactgtt gaggcaactc aacgcttgac tggtggtggc ggatttgcga ttggtcacca
     8461 acagcaattt gcaatctggt ttgtagataa agtagcagga cgctttggta agaaagaaga
     8521 aagtttagac aatcttaaat tacctaagtt cctctcaatc ttccacgata cagttgttgc
     8581 atctgctacc ttgatgctcg tattcttcgg agccattctt ttaatcttgg gtccagacat
     8641 tatgtctaat aaagaagtca tcacttcagg aactctattc aatcctgcta aacaagattt
     8701 ctttatgtac attatccaaa cagcctttac cttctcagtt tacttgttcg ttttgatgca
     8761 aggtgtccga atgttcgtat ctgagttgac aaacgccttc caaggtattt caaacaaatt
     8821 gttgccaggt tcattcccag cggttgacgt tgcagcttct tatggatttg gttctccaaa
     8881 tgctgtcctg tcaggattta tctttggttt gattggtcaa ttgattacaa ttgttttgct
     8941 catcgtcttt aaaaatccga ttcttattat tataggattt gtaccagtgt tctttgacaa
     9001 tgcagccatt gcggtctacg ctgataaacg cggcggatgg aaagcggctg ttatcctttc
     9061 ctttatatca ggtgtccttc aagttgctct aggagctctt tgtgtggccc ttctcgattt
     9121 ggcatcttat ggtggctacc atggaaatat cgactttgaa ttcccatggc ttggatttgg
     9181 atatatcttc aaataccttg gtattgttgg ttatgtactt gtgtgtctct tcttgcttgt
     9241 tattcctcaa cttcaatttg ccaaagcaaa agataaagag aaatattaca acggtgaagt
     9301 tcaagaagaa gcttagtatc tagaaaagga gaaataaaat ggttaaagta ttagcagcgt
     9361 gcggaaatgg aatgggttca tcaatggtta tcaagatgaa ggttgaaaat gctctccgta
     9421 agcttaatca aacagatttt acagtcaatt catgcagtgt cggtgaagct aaaggtttag
     9481 cagtaggata tgacatcgta atcgcttctc ttcatttgat tcaagaattg gaagggtgaa
     9541 ctaatgggaa gttaattggg cttgataact tgatggatga taaagaaatc accgaaaaac
     9601 tcagtcaagc actacagtaa aaggttggag ggggctggac agaaactgag agttatcgtt
     9661 tctgtccttc tccctcttta aataaaggag gcagatatga atttaaaaca agctttaatt
     9721 gacaacgact cgatccgact aggtttagag gctaacgatt ggaaagaagc agtcaaggta
     9781 gcagtagatc ccttgattga aagtggggca attttgccag agtattacga tgctatcatt
     9841 gaatcgactg aagagtatgg gccttactat atcttgatgc caggtatggc tatgccccac
     9901 gctagacctg aagctggtgt gcaaagtgat gccttttcat taattacctt acaaaatcct
     9961 gttgtatttt cagatgggaa agaggtatct gttttgttgg cactagcagc aacaagttca
    10021 aaaattcaca caagtgtagc cattccacaa attattgccc tgtttgaatt agaagattct
    10081 attgcacgtt tacaggcttg ccagactaaa gaagatgtct tggctatgat tgaagaatct
    10141 aaggatagcc cttatctcga aggattggat ttggaaagtt agaaagagga ataaagaaat
    10201 gacaaaaaga atacctaatt tacaagttgc attagaccat tcagacttgc aaggagcgat
    10261 taaagcagct gtttctgttg gtcaggaagt agatattatc gaagctggaa ctgtttgctt
    10321 gcttcaagtt ggaagtgaac tggctgaagt cttgcgtagc cttttcccag ataagattat
    10381 tgtggcagac acaaaatgtg ctgatgctgg tggaacagtt gctaaaaata atgcggttcg
    10441 tggagcagac tggatgactt gtatctgttg tgcaaccatc cctactatgg aagcagctct
    10501 aaaggctatc aagactgaac gaggagaacg aggcgaaatc cagatcgagc tttatggcga
    10561 ttggactttt gaacaagctc agctttggct agatgcaggt atctcacaag ctatttatca
    10621 ccaatctcgt gatgctcttc ttgctggtga aacttggggt gaaaaagacc ttaataaggt
    10681 taaaaaactc attgacatgg gcttccgtgt atctgtaaca ggtggtctag atgtagatac
    10741 tctcaaactc tttgaaggtg ttgatgtctt tacctttatc gcaggtcgtg gaattacaga
    10801 ggctgtggat ccagcaggag cagcgcgtgc cttcaaggat gaaatcaaac gaatttgggg
    10861 gtaaatcatg gtacgtccaa ttggaattta tgaaaaggca accccaacac actttacttg
    10921 gctagaacgt ttaaattttg ccaaggagtt aggctttgat tttatcgaga tgtctattga
    10981 cgaacgtgac gagcgtttag caagacttga ctggagtaag gaagaacgct tggaagttgt
    11041 caaagaaatc tatgaaactg gtgttcgtat tccttctatc tgtttttcag gccatcgtcg
    11101 ctacccattg ggatcaaaag attcagttct agaggaaaaa tctctagaac tcatgaaaaa
    11161 atgtatcgaa ttagctcaag acttgggagt tcgtacgatt caattagctg gttacgatgt
    11221 ttactatgag gaaaagtcac cccagacacg ccaacgtttt atcaaaaatt tgagaaaatc
    11281 ctgtgactgg gctgaagaag ctcaggtggt acttgctatt gaaattatgg atgatccttt
    11341 catcagtagc atcgaaaaat atttggctat agaaaaagag attgactctc ccttcctctt
    11401 tgtatatcca gatattggta atgtgtctgc atggcataat gatatctata gtgagtttta
    11461 tcttggtcat catgccatcg cagctctcca tctcaaggat acttatgcag tgacagaaag
    11521 ttcaaagggc cagttccgag atgtaccttt cgggcaaggt tgtgtcaaat gggaagaagc
    11581 tttcgatatt ttaaaggaaa ccaattataa tggacctttc ctaatcgaaa tgtggtctga
    11641 aaattgtgaa acagtagaag aaacacgcgc agccattcaa gaggcgcaag cttttctcta
    11701 tccactcatt aagaaagcag gtttgatgta agatgaatca agtaatcaat gctatgcgta
    11761 aacgagtctg tgatgccaat caatcattgc caaaacatgg acttgtcaaa tttacctggg
    11821 ggaatgtatc tgaagttaat cgcgaactcg gtgtcattgt tatcaaacca tcaggcgtgg
    11881 attatgacga attgacacct gaaaacatgg tagtgactga tctagatggt aagatcctag
    11941 aaggggattt aagaccatct tccgacctcc caactcatgt gcaattatat aaggcttggt
    12001 cagaaattgg tagtgtggtt cacacccatt cgacagaagc tgttggttgg gctcaggcag
    12061 gtcgtgatat tcctttctac ggaacaaccc atgcagatta tttctacggt tcaatccctt
    12121 gcgcccgtag tttgaccaag gacgaagtag aagtggccta tgaaaaagat actggcctgg
    12181 ttatcgtaga agagtttgaa catcgcggac ttaacccggt tgaagtacca ggaattgttg
    12241 tacgcaatca cggtccattc acctggggca aaaatccaga gaatgctgtt tatcactctg
    12301 tcgtactaga ggaagtatca aagatgaatc gctttacaga acaaatcaat ccaagagttg
    12361 gacctgctcc ccagtacata ctagaaaaac actaccaacg taaacatgga ccaaatgctt
    12421 attatggtca aaagtaagaa cgatgaagga ggagaaaaag ataaatttag ctcctctttt
    12481 tacatttgat ttttattgag agtaaagttg gaattgaagt aattttaaaa gattttttag
    12541 aaatagcgct tgatatatat atggtaaaat aaaaagaatt gctgtgatat caatagattt
    12601 gggggatttt ttaatatggt actggataag gcaagttgtg atttgcttca atatttgatg
    12661 gatcaagaaa cgtccaaaac gattatggcg atttcgaaag atttgaaaga gtcaagaagg
    12721 aaaatttatt atcacattga caaaatcaat gctgctctgg gtgacgaggc gcttcacatc
    12781 attagtattc cacgaattgg tattcactta acggaagagc agagagatgc ttgttgtaaa
    12841 ctattatcgg aagtagattc gtacgattat atcatgagtg cgcatgaacg tatgatgata
    12901 atgttactat ggataggtat ttctaaagaa cgtattacga ttgaaaaatt gatagagtta
    12961 acagaggtat ctaggaatac tgttctcaat gatttgaata gtattcgtta tcaactaact
    13021 ttggaacaat atcaggtgat cttgcaagtg agcaagtcac agggatacca ccttcatgcc
    13081 caccctctta ataaaattca gtatcttcaa tcgcttctat atcatatttt tatggaagaa
    13141 aatgccactt ttgtatctat tttagaagat aagatgaaag agaggttaga tgatgagtgt
    13201 ttgctttctg ttgaaatgaa ccaatttttt aaggaacagg ttcctttagt tgaacaagat
    13261 ttagggaaga aaataaacca tcatgaaata acttttatgt tgcaggttct accttatttg
    13321 ctgttaagct gtcataatgt tgaacagtat caagaaagac atcaggatat agagaaagaa
    13381 ttttctttga taagaaaaag aatagagtat caggtgtcta agaaattagg agaacggttg
    13441 tttcaaaagt ttgaaatttc tttgtcagga cttgaagttt ctcttgtagc tgttctcctc
    13501 ctctcctatc gtaaagattt ggatattcat gcagaaagtg atgattttcg gcaattaaaa
    13561 cttgctttag aagaatttat ctggtatttt gaatcacaaa tccgaatgga gattgagaac
    13621 aaggatgatt tgttacgaaa tttgatgatc cactgtaaag ccttgttatt tagaaagact
    13681 tacggtattt tttctaaaaa tcctctaaca aaacaaattc gatccaagta tggagaatta
    13741 tttttagtca ctagaaaatc tgcggaaatt ttagaaggag catggtttat tcggctaaca
    13801 gacgatgata ttgcctattt gacgattcat attggaggat ttttaaaata tacaccatcg
    13861 tctcaaaaaa atatgaaaaa agtttatctc gtttgtgatg aaggtgttgc ggtttcgaga
    13921 cttttgctga aacaatgcaa actttatttt ccaaatgagc aaattgacac tgtatttaca
    13981 acagaacaat ttaagagtgt ggaagatatt gcacaagttg atgtagtgat tactactaat
    14041 gatgatttgg atagcagatt tccgatttta agggttaatc ctatccttga agcagaagat
    14101 attttgaaaa tgctagacta tcttaaacac aatatatttc gtaataagag caaaagtttc
    14161 agtgaaaatc tttctagtct tatttcgtct tatattgtag acagcaagtt ggctagtaag
    14221 ttccaagaag aggttcaaac acttataaat caagaaatag tagttcaagc ttttttggaa
    14281 gatatttgaa ggacagtcca atgatgaaca caaacctgtg tttttcttgg tcttttttag
    14341 tgttttgaag ggtggtatac taatctcaaa gataacaatt atatccaaag gaggcaacat
    14401 atgccaaacg tcaaagaaat tacaagagag tcatggattt tagccacttt cccagagtgg
    14461 ggaacatggt tgaacgaaga aatcgaagaa gaagtcgtac ctgaaggcaa ctttgccatg
    14521 tggtggctag gcaactgtgg tacttggatt aagacaccag ctggtgctaa cgttgtcatg
    14581 gacctttggt caaaccgtgg aaaatcaacc aaaaaagtga aagatatggt tcgtgggcac
    14641 caaatggcaa atatggcagg tgttcgtaag ttgcaaccaa acttgcgtgt tcagccaatg
    14701 gttatcgatc catttgctat caacgaacta gactattact tagtttcaca cttccacagt
    14761 gatcatatcg acccatacac agctgcagca attctcaaca atcctaagtt agagcatgtt
    14821 aagtttatcg gtccttacca ctgtggacga atctgggaag gatggggtgt tccaaaagaa
    14881 cgtatcatcg ttgttaaacc aggtgacact atcgaattaa aagatatgaa gattcatgca
    14941 gtagaatcat ttgaccgtac ttgcttggta actctcccag tgaacggtgc tgatgagaca
    15001 ggcggtgaac ttgctggctt ggctgttaca gatgaagaaa tggctcaaaa ggctgttaac
    15061 tatatctttg aaacaccagg tggaaccatc tatcatggtg cagattctca cttctcaaac
    15121 tattttgcaa aacatggtaa aaactttaaa attgatgttg ctttgaataa ctatggtgaa
    15181 aatccggtag gtatccaaga caaaatgaca tctatcgacc ttcttcgtat ggcagaaaat
    15241 ctgcgtacca aagtcattat cccagttcac tatgatatct ggtctaactt catggcttct
    15301 actaatgaga ttctagaact ttggaaaatg cgaaaagatc gcttgcaata cgatttccat
    15361 ccatttatct gggaagttgg cggtaagtac acttatcctc aagatcaaca cttagtagaa
    15421 taccatcatc cacgtggttt tgatgattgt tttgaacaag actctaacat tcaatttaaa
    15481 gctttgctat aatataaaaa tattctttgg aggttatccg tatgttgcat gatacggata
    15541 attttcatat aatagtaaaa tagaatgtgt gattcaataa tcacctcaaa tagaaaggaa
    15601 attctatgtc aaatctatct gttaatgcaa ttcgttttct aggtattgac gccattaata
    15661 aagccaactc aggtcatcca ggtgtggtta tgggagcggc tccgatggct tacagcctct
    15721 ttacaaaaca acttcatatc aatccagctc aaccaaactg gattaaccgc gaccgcttta
    15781 ttctttcagc aggtcatggt tcaatgctcc tttatgctct tcttcacctt tctggttttg
    15841 aagatgtcag catggatgag attaagagct tccgtcaatg gggttcaaaa acaccaggtc
    15901 acccagaatt tggtcatacg gcagggattg atgctacgac aggtcctcta gggcaaggga
    15961 tttcaactgc tactggtttt gcccaagcag aacgtttctt ggcagccaaa tataaccgtg
    16021 aaggctacaa tatctttgac cactatactt acgttatctg tggagacgga gacttgatgg
    16081 aaggtgtctc aagcgaggca gcttcatatg caggcttgca aaaacttgat aagttggttg
    16141 ttctttatga ttcaaatgat atcaacttgg atggtgagac aaaggattcc tttacagaaa
    16201 gtgttcgtga ccgttacaat gcctacggtt ggcatactgc cttggttgaa aatggaacag
    16261 acttggaagc catccatgct gctatcgaaa cagcaaaagc ttcaggcaag ccatctttga
    16321 ttgaagtgaa gacggttatt ggatacggtt ctccaaacaa acaaggaact aatgctgtac
    16381 acggcgcccc tcttggagca gatgaaactg catcaactcg ccaagccctc ggttgggact
    16441 acgaaccatt tgaaattcca gaacaagtat atgctgattt caaagaacat gttgcagacc
    16501 gtggcgcatc agcttatcaa gcttggacta aattagttgc agattataaa gaagctcatc
    16561 cagaactggc tgcagaagta gaagccatca tcgacggacg tgatccagtc gaagtgactc
    16621 cagcagactt cccagcttta gaaaatggtt tttctcaagc aactcgtaat tcaagtcaag
    16681 atgccttgaa tgttgtggca gctaaattac caaccttcct aggtggatca gctgaccttg
    16741 ctcactcaaa catgacttat ataaagacgg atggacttca agacgatgcc aatcgcttga
    16801 atcgtaacat tcagtttggt gttcgtgaat ttgcaatggg aacaatcttg aacgggatgg
    16861 cccttcatgg tggacttcgt gtatacggtg gaactttctt cgtcttctct gactatgtga
    16921 aggcagctgt ccgcttgtca gccttacaag gacttcctgt gacttatgtc tttacccatg
    16981 attcaatcgc agttggggaa gatggtccga ctcacgaacc agttgagcat ttagcaggtc
    17041 ttcgtgctat gccaaatcta aatgttttcc gtccagcaga tgcgcgtgaa actcaagcag
    17101 cttggtacct tgcagtgaca agtgagaaaa caccaactgc ccttgttttg acacgtcaaa
    17161 acttgactgt tgaagatgga acagacttcg acaaggttgc taaaggtgct tatgttgtat
    17221 atgaaaatgc agccgacttt gataccatct tgattgcgac aggttcagag gttaatcttg
    17281 ctgtctcagc tgctaaagaa ttggctagtc aaggcgaaaa aatccgcgta gtcagcatgc
    17341 catctacaga tgtctttgat aaacaagatg cagcttacaa ggaagagatt cttccaaatg
    17401 cagtccgccg tcgtgttgca gtcgaaatgg gtgcaagtca aaactggtac aaatatgttg
    17461 gtctcgatgg tgccgttcta ggtattgata ccttcggagc ctctgcccca gcaccaaaag
    17521 tattggcaga atatggcttt actgtagaaa atcttgtaaa aattgttcga aacttgaaat
    17581 aatcctaaaa atcagggcgt aagctctggt ttttcttacc agaaaagtaa ggtacaatct
    17641 tgtaaaagta gctgaaattt gatatagtag tcctatgtaa aagacaaagg agaaaacaat
    17701 gaatccaaat attacttttt taatcatgct tgtaggtatg atggccttga tgttctttat
    17761 gcaacgttct caaaagaaac aagctcaaaa gcgtatggaa agcttaaata aactacaaaa
    17821 aggctatgaa gtgattacaa tcggtggact ttatggaaca gtcgatgaag tagatacgga
    17881 gaagggaaca atagttcttg acgtagatgg agtttacttg acttttgaac tagctgctat
    17941 caagacagta ttaccgctga aagaaacagc ttcactagaa ggcgcaattg aaaaataaga
    18001 cgggatcacg aactcccgtt tttctataaa agaaaggaaa tgggatgaaa aaattagtct
    18061 ttgtctgtct gggaaatatt tgccgtagcc ctatggccga gtttgttatg aaatcaatga
    18121 cagataacta cgaaatccaa agtcgagcaa cttcctcttg ggaacatggc aatccgattc
    18181 ataaggggac tcagggaatt tttcaagagt atgagattcc ttatgacaag aacaagacat
    18241 cgcttcagat tagtaaggaa gattttgaag cctttgatta tattatcgga atggacgctt
    18301 caaatgtttc cgacttacgt cagatgtgtc cagtagactg tcaagataag atttactcat
    18361 tttcatctga aagtgttcca gacccttggt atacaggaga ttttgaagaa acctatcgac
    18421 gtgttcaaga gggttgtcaa gcttggttag aacgtttaga aaaggagagt taaggtggaa
    18481 aatcttacga atttttacga aaagtatcgt gtttatctga ctcgtccacg tttagagatt
    18541 ttggcagtag ttaccattgt tttctgtgct gtactcgtct tttttctaaa tattccagga
    18601 aaaggtgtct taaaactcga taatggaacg attgtttatg atggcagtct tgtccgcggt
    18661 aaaatgaatg gccaaggtac cattaccttc caaaatggag accaatatac aggtggcttc
    18721 aacaatggag ccttcaacgg aaaaggtacc tttcaatcta aagaaggctg gacctacgaa
    18781 ggtgattttg taaatggtca ggctgaagga aaagggaaac taacaacaga acaagaagtc
    18841 gtttatgaag gaacttttaa acaaggcgtt tttcaacaaa aataaagcct ccttatcaaa
    18901 ggaggtatta ttagaatcgc aaggtaagcg cttacttgca aatccctttc tttccaaatc
    18961 cctcttccaa ccaagtttgt gaaataaaaa atatttgaaa taaatttcac aaacttcaaa
    19021 gataaaacct gataagaaaa gaaaatgaga aaagtttcac aagagtttaa aaattctttg
    19081 tgaaatgttt atgaaactgt ttacaaagtt gaaataatac gttataataa acttgtgaaa
    19141 aaattaacaa aggatatcgt tccttgaaag ctatggagga aaatatggct gataaaaaaa
    19201 ctgtgacacc agaggaaaag aaactcgttg ctgaaaaaca cgtagatgag ttggttcaaa
    19261 aagctctagt tgcccttgaa gaaatgcgta aattggatca agagcaagtt gactacatcg
    19321 ttgccaaagc atcagtagca gctttggatg cccacggaga attggcttta catgcctttg
    19381 aagaaacagg acgtggtgta tttgaagaca aagcaactaa gaacttgttt gcctgtgaac
    19441 acgtagtaaa caacatgcgc cacactaaga cagttggcgt tatcgaagaa gacgatgtaa
    19501 caggattgac tcttattgct gaaccagttg gtgttgtttg tggtattact ccaacaacaa
    19561 acccaacatc aacagcaatc ttcaaatcat tgatttcatt gaagacacgt aacccaatcg
    19621 tctttgcctt ccatccatca gcacaagaat catctgctca tgcagctcgt atcgtccgcg
    19681 atgcagctat cgcagctggt gctcctgaaa actgtgtgca atggattact caaccatcta
    19741 tggaagcaac aagtgccctt atgaaccacg aaggtgttgc gacaatcctt gcaacaggtg
    19801 gtaatgccat ggttaaggcg gcttattcat gtggtaaacc agctcttggg gtaggtgccg
    19861 gaaacgttcc agcttatgtt gaaaaatcag caaacattcg tcaagcagca cacgatatcg
    19921 tcatgtctaa atcatttgat aacggtatgg tctgtgcatc tgaacaagca gttatcattg
    19981 ataaagaaat ttacgatgaa tttgtagcag agttcaaatc ttaccacact tactttgtaa
    20041 acaaaaaaga aaaagctctt cttgaagagt tctgcttcgg cgtcaaagca aacagcaaaa
    20101 actgtgctgg tgcaaaattg aacgctgaca tcgttggtaa accagcaact tggattgcag
    20161 aacaagcagg atttacagtt ccagaaggaa caaacattct tgctgcagaa tgtaaagaag
    20221 ttggcgaaaa tgagccattg actcgtgaaa aattgtcacc agttattgca gttttgaaat
    20281 ctgaaagccg tgaagatggt attactaagg ctcgtcaaat ggttgaattt aacggtcttg
    20341 gacactcagc agctatccac acagctgacg aagaattgac taaagaattt ggtaaagctg
    20401 ttaaagctat tcgtgttatc tgtaactcac cttctacttt tggtggtatc ggggacgttt
    20461 acaatgcctt cttgccatca ttgacacttg gatgtggttc ttacggacgc aactcagttg
    20521 gggataacgt tagtgccatt aacctcttga atatcaaaaa agtcggaaga cggagaaata
    20581 acatgcaatg gatgaaactt ccttcaaaaa catactttga acgtgattca attcaatacc
    20641 ttcaaaaatg tcgtgacgtt gaacgtgtca tgatcgttac tgaccatgcc atggtagagc
    20701 ttggtttcct tgatcgtatc atcgaacaac tggaccttcg tcgcaataag gttgtttacc
    20761 aaatctttgc ggatgtagaa ccggatccag atatcacaac tgtaaaccgt ggtactgaga
    20821 ttatgcgtgc cttcaaacca gataccatca tcgcactcgg tggtgggtct ccaatggatg
    20881 ctgccaaagt aatgtggctc ttctacgagc aaccagaagt ggacttccgt gaccttgtcc
    20941 aaaaattcat ggatatccgt aaacgtgcct tcaagttccc attgcttggt aagaagacta
    21001 aattcatcgc gattccaact acatctggta caggatctga agtaacacca tttgccgtta
    21061 tctctgataa agcaaacaac cgtaaatacc caatcgctga ctactcattg acaccaactg
    21121 tggcaatcgt agatcctgct ttggtattga cagttccagg atttgttgct gctgatactg
    21181 gtatggacgt attgactcac gcgacagaag catacgtatc acaaatggct agtgactaca
    21241 ctgatggttt agcacttcaa gccattaaat tggtctttga aaatctcgaa agctcagtta
    21301 agaatgcaga cttccactca cgtgagaaaa tgcataacgc ttcaacaatc gctggtatgg
    21361 cctttgccaa tgccttccta ggtatttctc actcaatggc ccataagatt ggtgcgcaat
    21421 tccacacaat ccacggtcgt acaaatgcca tcttgcttcc atacgttatc cgttacaacg
    21481 gtacacgtcc agctaagaca gcaacatggc ctaagtacaa ctactaccgt gcagatgaaa
    21541 aataccaaga tatcgcacgc atgcttggac ttccagcttc tactccagaa gaaggggttg
    21601 aatcttacgc aaaagctgtc tacgaactcg gtgaacgtat tgggatccaa atgaatttta
    21661 gagaccaagg aattgacgaa aaagaatgga aagaacattc tcgtaaatta gccttcctgg
    21721 cttatgaaga ccaatgttca ccagctaacc cacgtcttcc aatggtagac catatgcaag
    21781 aaatcatcga agatgcatac tatggctaca aagaaagacc aggacgccgt aaataattgt
    21841 ttatcagtct agaagcaaga caaaaactca atttgaggga aagatccagt aatttttcta
    21901 tgataaaagg catcctatca aggtttttga acacctgata ggatgccttt ttatgatatt
    21961 gaggcctttt tgcccttttt gaaaaactag aatagaaaca aaatatataa tagattgaaa
    22021 ctagaatagt acatatctgc ttctaaaaca ttgttagaat tcgatttgac tgtcctgatc
    22081 gatttgtcct gttcttattt cattttgata tataaaaaat atagtatagt agactgaatc
    22141 taaaatagta cgaaacaatt gctaaa
//
LOCUS       DAVKWZ010000033        21491 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000033 DAVKWZ010000000
VERSION     DAVKWZ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21491
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.033"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(88..1107)
                     /gene="adhP"
                     /locus_tag="VW854_001293"
     CDS             complement(88..1107)
                     /gene="adhP"
                     /locus_tag="VW854_001293"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEV0262525.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWAVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
     gene            complement(1446..2258)
                     /locus_tag="VW854_001294"
     CDS             complement(1446..2258)
                     /locus_tag="VW854_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV0262526.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKKTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKEMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            2448..3866
                     /locus_tag="VW854_001295"
     CDS             2448..3866
                     /locus_tag="VW854_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEV0262527.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLVITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            3919..4626
                     /locus_tag="VW854_001296"
     CDS             3919..4626
                     /locus_tag="VW854_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0262528.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVTGLSIVVLALFIMGARKTKLASFNFSFFRAKDLARLGLSYLVIVGSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            4721..5695
                     /gene="folP"
                     /locus_tag="VW854_001297"
     CDS             4721..5695
                     /gene="folP"
                     /locus_tag="VW854_001297"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEV0262529.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALATGADLVNDITGLMGDEKMAHVVAKAGAKVVIMFNPVMARPQHPSSLI
                     FPHFGFGQTFTEKELADFETLPIEDLMVAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEESGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            5697..7019
                     /locus_tag="VW854_001298"
     CDS             5697..7019
                     /locus_tag="VW854_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV0262530.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGRIAPEALTVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALGDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            7000..7554
                     /gene="folE"
                     /locus_tag="VW854_001299"
     CDS             7000..7554
                     /gene="folE"
                     /locus_tag="VW854_001299"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEV0262531.1"
                     /translation="MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            7597..8409
                     /gene="folK"
                     /locus_tag="VW854_001300"
     CDS             7597..8409
                     /gene="folK"
                     /locus_tag="VW854_001300"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEV0262532.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIYILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(8454..8771)
                     /locus_tag="VW854_001301"
     CDS             complement(8454..8771)
                     /locus_tag="VW854_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEV0262533.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            9128..9574
                     /gene="rplM"
                     /locus_tag="VW854_001302"
     CDS             9128..9574
                     /gene="rplM"
                     /locus_tag="VW854_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEV0262534.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            9593..9985
                     /gene="rpsI"
                     /locus_tag="VW854_001303"
     CDS             9593..9985
                     /gene="rpsI"
                     /locus_tag="VW854_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEV0262535.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVIGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(10273..11427)
                     /locus_tag="VW854_001304"
     CDS             complement(10273..11427)
                     /locus_tag="VW854_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV0262536.1"
                     /translation="MSIHKYKTKKGVLYYVSFYIGLDAYGKKKRHLKRGFKTKKEAKL
                     YEARLEAGVVAPTVNTDKPNPKVTYKEFYQEWFKAYVGTVEETTSSQTKNIYRIHILP
                     IFGGKFIDQISPLDCQNFITQKSQTFKNIKQIKSYTSKIFDFAINMNYIDRNPMKNVI
                     MPKIKKTRSDNFWSLEELHHFLDIVKETEPFKHFALFRLLAFSGLRKGELYALKWADI
                     NFDSNLLNIDKSLGRIDGKAIEKSTKNESSVRTIYLDDETIDIIKQWRNFYLKEQSQF
                     PLTKLNISNEYMFYYISSNDKIEPLHADYINNVLNRIIKKHKLKPISPHGFRHTHATL
                     MSEIGIDPSNTSKRLGHASSQMTLDVYTHTTKTGEKNSIDKFADYLNKAK"
     gene            complement(11479..11997)
                     /locus_tag="VW854_001305"
     CDS             complement(11479..11997)
                     /locus_tag="VW854_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0262537.1"
                     /translation="MSNHITKLIEDSGKKLTEISEATDIAYPTLSGYNQGIRKPKKDN
                     AEKLAKYFNVSVAYIMGLDSNPHAPSNLKIVTDSFKTSKIGSVTPFKSDMEKLKKSIE
                     FGERALTLPLDDSFSDEFRHIFSEYLAEKNKRFMSEFIDYMNHQNKDSKVWQSWIKTD
                     EYAIRQEDRKNR"
     gene            12145..12426
                     /locus_tag="VW854_001306"
     CDS             12145..12426
                     /locus_tag="VW854_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV0262538.1"
                     /translation="MSETTLVVQLPKELEKQLRAHYDEMISNAVARVFEDKELYKPMV
                     RMSGLSRWLDVSTTTIQKWTKEGMPTMVIDGVTLYDKRAVTRWLKQYER"
     gene            12435..13274
                     /locus_tag="VW854_001307"
     CDS             12435..13274
                     /locus_tag="VW854_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEV0262539.1"
                     /translation="MNGVQWIKITTDIFDDEKIQLIESMPEGDTLIVIWFKLLVLAGK
                     QNNSGILSLGNKVYYTEEMLSTVFRRKATSVKLALSMFEEFGMIEIIDGAITIPKWEK
                     HQNIDGLEKIRKQTRERVARHRQKQKALIKGEPQLILDCNVTVTDENENVTQEIKNES
                     KNKKEIENILDNSSRKPETYIPPKYYSLLESISYKYNDRFLYPNNYTLTHAQKMKIGE
                     YLASGYVTSDEVISMIERIPEDAASPLAYLFKSMENLKQERMLECKTIAHENARKKYM
                     INE"
     gene            13267..13626
                     /locus_tag="VW854_001308"
     CDS             13267..13626
                     /locus_tag="VW854_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0262540.1"
                     /translation="MSRYRTILKKFYITEEQNEIANNLIKMTNHLSFSSYARKMLFKR
                     SPIYLQFDFKSYHDFIFQVRRIINNLRQLERIAKQSEDLDNVRIFHCCVEMMIGYEKK
                     TSKQVKELVKRLNKKTR"
     gene            13634..13966
                     /locus_tag="VW854_001309"
     CDS             13634..13966
                     /locus_tag="VW854_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262541.1"
                     /translation="MRIKSVLKQVFLTEEENKKLNDCMRKENICNFSEFARKKLIRTD
                     LNIHKVSFEGLVPLTEELEQVGKNINSIARLATVVGRISYENKMDMSIMMQKIVDVME
                     EKDVYFQK"
     gene            13947..14141
                     /locus_tag="VW854_001310"
     CDS             13947..14141
                     /locus_tag="VW854_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262542.1"
                     /translation="MFIFKSSMDKDFDQICEYILKLKRAELFLHGIKQQDKTKARLYH
                     AMLEYYDANHKKVRHMRILK"
     gene            complement(14532..14828)
                     /locus_tag="VW854_001311"
                     /pseudo
     CDS             complement(14532..14828)
                     /locus_tag="VW854_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            15236..15904
                     /locus_tag="VW854_001312"
     CDS             15236..15904
                     /locus_tag="VW854_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001158457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEV0262543.1"
                     /translation="MQETMIDYKQKIYQELSRLTKGLGSEKRLDILNILSQGPKTVEG
                     IAHTTGLSVANTSRHLQILKESHLVVTSRNGNFIRYSLASEKVVQLVLLLFAVGEEQL
                     AEVRAIESDYDEAAGVPQIELANAIELAAKTDALLLDLRPVEEYEAGHLPKALNLPID
                     EFDQKKAQLPKEKTIIVYCRGRQCGYANLAAQDLQSLGYSIYSLNRSFADWKWGASSR
                     SELD"
     gene            16210..16524
                     /gene="trxA"
                     /locus_tag="VW854_001313"
                     /pseudo
     CDS             16210..16524
                     /gene="trxA"
                     /locus_tag="VW854_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889674.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
     gene            16533..17453
                     /gene="trxB"
                     /locus_tag="VW854_001314"
     CDS             16533..17453
                     /gene="trxB"
                     /locus_tag="VW854_001314"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEV0262544.1"
                     /translation="MYDTIIVGSGPAGYTAGIYLSRAGLKNRLITGYSEGGQLTTTTL
                     VENYPGFENGIEGNKLVQTMRRQAEQFGTEMTFGFVQKIEGESSPFKVHLDNGEIIDT
                     KAVIIATGSSAKYLGIEGEEEAVGKGVSACATCDAFFYKEREVLVVGGGDVAMEEALY
                     LTRFASKVTVVHRRNELRASDIMVKRAQANDKIHWMLNYTPVKVASNLMGMTGLDVRN
                     NETGDIEHIQADGLFVAIGHQPNTDFLDGKLALDDKGYIQTQAGTAKTSVAGIFAAGD
                     VQDPKYQQAITAAGSGAIAALDTLAYIDNL"
     gene            17450..17719
                     /locus_tag="VW854_001315"
     CDS             17450..17719
                     /locus_tag="VW854_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014677713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262545.1"
                     /translation="MMDSVTSSIELEELLDEHQNLALLVSGNQCSLSSLGQKELIDTA
                     EREHLVLYQADIFQVPRLISLFGIQPTLTSLLVFKKGQLVDRKEV"
     gene            17720..17899
                     /locus_tag="VW854_001316"
     CDS             17720..17899
                     /locus_tag="VW854_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003156488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEV0262546.1"
                     /translation="MARKALIQRKKKAYPVQEYSRCLACGRPHSVYRKFKLCRICLRQ
                     QAYQGVIPGLKKASW"
     gene            17923..18573
                     /locus_tag="VW854_001317"
     CDS             17923..18573
                     /locus_tag="VW854_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HEV0262547.1"
                     /translation="MEISYWSDIACPFCYIGASRMKRAMAAVGLNPEDLTMKAYQLNP
                     NAPLETSETMLTEFATSHGMTTEQVKAQFAHMESMGNEEGLRIDMAGIIPTNTFSAHR
                     LIKWSQKYLDKKDHQNFITALYYLYFEEHANIADHSVLLAVISEFDLPQEEAAQVLAG
                     DAFADEVKRDILEAHQSGVQGAPFFVLNNKYGISGAQPYEYMLATLKKIQAEEGAQ"
     gene            18575..18751
                     /locus_tag="VW854_001318"
     CDS             18575..18751
                     /locus_tag="VW854_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262548.1"
                     /translation="MIDTPFQLLDSNSEETGLVCGIDGCSIDPSTQPLENTYQPLEKS
                     KKAVIGRTEWKNKE"
     gene            18733..19296
                     /locus_tag="VW854_001319"
     CDS             18733..19296
                     /locus_tag="VW854_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEV0262549.1"
                     /translation="MEKQRVEALSDAILAIIVTIMALELQLPEELTVAGLRSMLPMLF
                     IYITSFLQIMAVWLYYHELYKLVDHVSFHLFGANSFWLLTASFVPLATRGIGQNSANF
                     AFLLFYLIVLALWDVSWIIMTAMVIKTSQRQLSAEEKKIPYRWALLYGLQLVIILLVG
                     YLFPNFIPFAPLLMIINVIYGFLSNKS"
     gene            complement(19446..19664)
                     /locus_tag="VW854_001320"
                     /pseudo
     CDS             complement(19446..19664)
                     /locus_tag="VW854_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            19845..21311
                     /locus_tag="VW854_001321"
     CDS             19845..21311
                     /locus_tag="VW854_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262550.1"
                     /translation="MFSKLSDHDFKKGVFRTKFSQLVTPLGKESDWAHSKGPEYLWIG
                     LALQYGNRIEQMERVMLALTNLSKALDLEKILPLPAMSLILNLDIDEKKIFVESLNSA
                     FDLSIFSPLSIILPDSEEVLSKNFYSKSHSFNQRLDILVKVLNEVSDQYSQLSTDVRY
                     FLLYYKMLQGKIQFVESQLHMVDGLSKYPYLDISDPEMRIIRPQIRSMEVALSMSEDM
                     NYPYSKRFWNNISQLTDCEVYSIVMDRSNTIDLNKIKDTVFSTLKYYRDMLQSIEPFN
                     EKLYVLTSILTYSYKRLLELMNHGLQYTISGRSIVRSCIENYVMTKYLIAEEEKHKNI
                     WEEYQYYGIGGYKLISERYIENQPELTNSHINFEYLNLLVSEFQNKEFIDMDTRYFGK
                     GNIRQKFEQVEEDDLYKYHYDYDSQFEHGLWGAIRESSILKCNSAGHQFHGIPDIDDI
                     QKLPDVSHDLVMVLTKHLSVIKEVFPIPSEQIEGDSLC"
BASE COUNT         6574 a         3729 c         4516 g         6672 t
ORIGIN      
        1 ttgaggagta atcttgaatt cttatttaaa atttaatata aaaaagttgg aagaagcttc
       61 caacttttaa aataggtttc tattagatta gtgggtgaag tcgagtacca tacgtccttg
      121 gatttggcct ttttccattt cgtcgaaaat ggcaacagca tcttctactg gacgtttttg
      181 aacaactggg actaccagac cttctgcacc aaattggaag gcttcttcta agtctttacg
      241 agttccgaca agagaaccga tgacttggat tccatcgagg actgttttaa cgatgctgag
      301 ttccatcatt tcagaaggaa gaccaacagc gacgacgcga ccaccagcac gaatggagtc
      361 aacagcctgg ttgaaggcaa ctttagacac agcagttacg acagctgaat gagctcctcc
      421 atcagttttt tctttaatga gtccagctac atcttcaact tcgaggccgt taatcacaat
      481 gtcagcgcct acttcttttg caagggcaag tttgtcattg ttgatatcga ctgcgataac
      541 atgagcattg aatacttttt tagcgtattg aacagcgagg ttaccaagtc caccagcacc
      601 gtaaagaaca gcccattggc ctggttcaac ttttgcttct ttgatagctt tataggttgt
      661 tactccagca catgtgatag aagaagcttg ggctggatca agtccgtcag gaactttgac
      721 agcatagtca gcagttacga tacattgttc agccatacca ccgtctactg agtagccagc
      781 atttttcact gtacggcaaa gggtttcgcg accagttgta cagtattcgc aagtgccaca
      841 tccttcaaag aaccaagcaa cgctgacgcg gtcaccgact ttaaggcttt tcacatctgg
      901 agcaatctct ttaacgatac cgataccttc gtgcccaaga acacgtcctg ggacttgacc
      961 aaagtcacca tgagcaacgt ggaggtcggt gtggcaaacg ccacagtatt caacttctac
     1021 aagtgcttcc ccagtttcaa gtggacggag tactttttct tcaatagcaa caccagtgct
     1081 ttctggattt acaacaacag ctttcatagc tatcctcctt gatattgacc gagaacaggg
     1141 acaacgggac tggattgatt ttttgtcaac agagtcgagt gtgacgagct ctcttgtatt
     1201 tatatgtgaa gtatatcaca aaagaaagcc ttttccaagg ttttggagac aaaaagtaga
     1261 acaatcgatt aactggttga tgagactgtg aaaagagcac aaagaccagc ttgcaagctg
     1321 gtcatactct tcgaaaatct cttcaaacca cgtcagcttc atctacaacc tcaaagcagt
     1381 gctttgagca acctacggct agcttcctag tttgctcttt gattctcatt gagtatcagt
     1441 ataggttata agaataagtc ttgtagggtt ttggcaaccc catcttggtc gttggtaagg
     1501 gaaatttgct catctgcata agggagtagc tctggattgg catttttcat ggcataaccc
     1561 ttcccagcaa aagcgagcat ttcggtatca ttgtgttcat ctccaaaggc aatcaaatct
     1621 tttttgtcac gattcattat cttgagcaag tagtccaaag caaaggcctt gttgacacct
     1681 tttggggtac attcaaggat attgagcgga cctccccagg tattgataga aagttgatgc
     1741 tggtagaagg cgttcatttc ttttgccaag gcatatttgt cactggctct ggtctgcaag
     1801 aggatacagt tagggtcctt ggtcaccaat tcaggctgga attgatcttc aggctggaaa
     1861 gcttctacac caaatagttt gggattggca atttcttcat tgggatttgt aatgtagaat
     1921 tttttacgat attctccagc gataaaatcg gcttgaatgt cctctgaacg ttgaaccata
     1981 tctagcagat attttttgtc tacagtcaaa cacttttcaa aatcccaaac ttggtctggt
     2041 aaatgagtaa gggatccgtt gaagttaatc ataggagtgt ctaagcccag ttcacggtaa
     2101 aaatcttttg acatacggta agggcgacct gtcgtaataa taacctgatg gcctttttca
     2161 gcaacttttt tgatggtttt tttggtaaag tcagaaattt gactgtctga gttgagcagg
     2221 gttccatcta ggtcaactgc aataattttt ttcgtcatat ggacctttct agtgtgtttt
     2281 cagataagat gtcttctatt ataacagaaa gcaggcagaa aagcagattc caaacaaaaa
     2341 atgaaattgg attaatttct taaacaaatc aaacaaagac attgaaaaag tgaatgataa
     2401 atgatataat tctattattg ttcgtaaaaa ttaaaaggag attgatgatg gacaaattat
     2461 ttaaactaaa agagaacggt acagacgttc gtacagaggt tctcgctggt ttaacaactt
     2521 tctttgcaat gagctatatt ctctttgtaa acccacaaat tctttcgcaa acaggaatgc
     2581 ctgctcaggg tgtgtttctt gcaacgatta tcggtgcagt tgctggtacc ttgatgatgg
     2641 ccttctatgc taacttgcca tacgctcaag caccaggtat gggactcaat gccttcttta
     2701 cctttacagt cgtatttgga cttggttatt catggcaaga agccctagct atggtcttca
     2761 tctgtgggat tatttcattg gttattacct tgacaaatgt tcgtaaaatg atcattgaat
     2821 cgattccaaa tgcccttcgc tcggctattt cagctggtat cggtgtcttc cttgcctatg
     2881 tagggattaa gaatgctgga cttttgaaat ttacgattga tccaggcaac tatactgttg
     2941 taggagaagg ggctgacaaa gctcaagcaa cgattgcagc aaactcttca gcagttccag
     3001 gcttggtcag ctttaataat ccagctgttt tagtggctct tgcaggactt gccattacta
     3061 tcttctttgt catcaaaggg attaaagggg gaattattct ctctatcttg acaacaactg
     3121 ttcttgctat tgcagttggt ttggttgatt tgtctagtat cgattttgct aataaccatg
     3181 ttggtgcagc ttttgaagat ttgaagacaa tctttggtgc agctcttggt tcagaaggtt
     3241 tgggagcttt ggtttcagat acagctcgct tgcctgaaac tctgatggcc attcttgcct
     3301 tctcattgac agatattttt gacacaattg gtaccttgat cggtacaggt gaaaaagttg
     3361 gtattgtagc gacaaatggt gaaaatcacc aatcagccaa attggataag gctctttact
     3421 ctgatttgat tggaacgaca gtcggtgcca ttgcaggtac ttcaaacgta acgacttatg
     3481 ttgagtctgc tgctggtatc ggtgcaggtg gacgtactgg tttgacagcc ttggttgtag
     3541 ctatctgttt tgcgatttca agcttcttta gcccacttct agcgatcgta ccaacagcgg
     3601 ctacagctcc aatcttgatt atcgttggga ttatgatgct tggtagcttg aaaaatatcc
     3661 attgggatga tatgtctgaa gcagttcctg ccttcttcac atctatcttt atgggattca
     3721 gctactctat cactcaaggg attgcagttg gtttcttgac ttacactttg actaagcttg
     3781 tcaaaggtca agttaaagat gttcatgcca tgatttggat tttggatgcc ttgtttatcc
     3841 ttaactacat cagcatggcc ttataataga atgacccagg gggatttccc ccctttttta
     3901 atacaaagga gataggtgat gaaagagaaa aatatgtgga aagaattgtt gaatcgtgca
     3961 ggctggattt tggtcttttt acttgccgtc cttttatatc aggttcccct agtggttacc
     4021 tctattttga ctttaaaaga agtagccctg ctacagtcag ggctgatagt tactggcctt
     4081 tcaattgtgg ttctggctct atttattatg ggagctcgta aaaccaagtt agctagtttt
     4141 aatttttctt tttttagagc taaagatttg gcacgtttgg gcttgagtta tttagttatt
     4201 gtcgggtcaa atatacttgg ttctatcttg ttgcaactgt caaatgagac gacaacagct
     4261 aaccagtctc agattaacga tatggttcag aatagttctc tgatttctag cttcttcttg
     4321 ctagccttgc ttgctccaat ttgtgaggaa atcttgtgcc gtgggattgt tcctaaaaag
     4381 attttccgag gcaaggagaa cttgggattt gtagtcggta cgattgtgtt tgctttattg
     4441 catcaaccaa gtaatttacc ttctttattg atttatggag gtatgtcgac agttctatct
     4501 tggacggcct acaagaccca acgtttggaa atgtcgatct tgcttcacat gattgttaat
     4561 gggattgctt tctgtttgtt ggctcttgtg gtgattatga gtcggacatt aggaatttct
     4621 gtttaaaagt ttttatgtag gaaccgacct ctttctacta gggaaagatg aatgcaatcg
     4681 tgtccatctt tttcttttta tggtaaaata gaaaaataat atgatgaaaa tccttgaggg
     4741 agtgaccgat atgtcaagta aagccaatca tgcaaagaca gttatttgcg gaattatcaa
     4801 tgtaacccca gactcctttt cggacggtgg tcaatttttt gctcttgagc aggcactcca
     4861 gcaggctcgt aaattgatag cagaaggagc cagtatgctc gatatcggcg gagaatcgac
     4921 tcggccggga agtagctatg ttgagataga agaggaaatc cagcgtgttg ttccagtgat
     4981 caaagcgatt cgcaaggaaa gtgatgtcct catctctatt gatacttgga agagtcaagt
     5041 agcagaggct gctttggcta ctggtgccga tctagtcaat gatatcactg gtctcatggg
     5101 tgatgaaaaa atggcccatg tggttgctaa agctggagcg aaagtggtca tcatgtttaa
     5161 cccagttatg gctcgacctc agcatcctag ttcgcttatc ttccctcatt ttggctttgg
     5221 tcaaaccttt acagaaaaag agttagctga ctttgaaaca ttgccaatcg aagacttgat
     5281 ggtggctttc tttgaacgag cactagcgag agcggcagaa gctggtattg caccagaaaa
     5341 tatcctgttg gatccaggaa ttggctttgg tctgaccaag aaagaaaatc ttctcctttt
     5401 acgggacctg gataaactac atcagaaggg ctatccaatc tttctcggag tgtcgcgcaa
     5461 gcgatttgtc atcaatatcc ttgaagaaag tgggtttgaa gtcaatcctg agacagagct
     5521 tggtttccga aatcgggaca cggcttcggc tcatgtaact agtatcgctg cgagacaggg
     5581 tgtagaagtg gtgcgcgtgc atgacgtagc tagtcacagg atggcagttg aaattgcctc
     5641 tgccattcgt ctggctgatg aagcggaaaa tttagattta aaacaatata aataagatga
     5701 aagaaattga aaacaatcag tggattgcta actaccggac ggatcaaccg cattttggct
     5761 tggaacgaat ggtggaactg ttagctttgc gtggcaatcc ccatcttaaa ctcaaggttc
     5821 tccatatcgg agggactaac ggcaagggct cgactattgc ttttttgaaa aagatgctag
     5881 aaaagctagg gctgagagtt ggcgtgttta gttcgcccta tctcattcat tacacagacc
     5941 agattagcat caatggggaa tcgatctcag aagcgaggct agaagctctc atggcagact
     6001 atcagtcttt gctggaggga gaagcggtcg ccaatttaca gggcacaacc gagtttgaga
     6061 ttatcacagc cctagcctat gactactttg cctcagagca agtagatgtg gctatcatgg
     6121 aagttggcat gggtggactt ttggatagta ccaatgtctg tcagcccatt ttgacaggaa
     6181 ttacaactat tggcttggat catgtggccc tacttggtga caccttggag gccatagcag
     6241 agcagaaggc aggtattatc aaacaaggga tgcccttggt aacagggcgt attgctccag
     6301 aagccttgac tgtgattgac cgcattgcgg aagggaaaga tgcgccgaga cttgcctacg
     6361 ggacagatta tcaggttcgt catcaagaaa gtgtggtgac aggggaagtc tttgactata
     6421 caagtgctgt cagacaaggt cgcttccaga ctagcctgct tggtttgtac caaatagaga
     6481 atgctgggat ggccatagct ttacttgata ctttttgtca agaagatggt cgagagctag
     6541 caagcaatga ctttcttggt caagccttgg aagaaacaag ttggccaggg cgtttggaaa
     6601 tcgtgtcaag agatcccttg atgattttgg atggagccca taatccccat gctatcaagg
     6661 ccttgttggt aaccttgcaa gaacgttttg cggattatca taaggaaatc ctcttcactt
     6721 gtatcaaaac caaagccttg ggggatatgt tggacttgct gggagccatg ccagataccg
     6781 agcttactct aacacatttt gcggatagtc gggcgacgga tgaaagcgtg ctgaaagagg
     6841 cagctaagtc tagaaatctc agctaccaag attggcatga ttttctagag cagaatttga
     6901 cagataaaaa agaagagaaa caaacagtta ggattgtcac aggttccttg tatttcttga
     6961 gccaagtgag ggcctatctg atggagagga agaacgagaa tggatacaca aaagattgaa
     7021 gcagctgtaa aaatgattat cgaggctgta ggagagaacg ctaatcgcga gggcttgcag
     7081 gaaacacctg ctcgtgtagc ccgtatgtat caagagattt tttcaggtct tggtcaaaca
     7141 gcagaggaac atttgtcaaa atcctttgaa attattgacg ataatatggt ggtagaaaag
     7201 gatatctttt tccataccat gtgtgaacac cacttcttgc cattttatgg tagagcgcac
     7261 attgcctaca ttccagatgg tcgtgtggca ggcttgtcta agctagcccg tacggttgaa
     7321 gtttattcga aaaaaccaca aattcaagaa cgtttgaata tcgaagtggc cgatgccttg
     7381 atggactatc taggtgctaa aggagccttt gttgtcattg aggcggaaca tatgtgtatg
     7441 agtatgcgtg gtgttagaaa accaggcact gcaaccttga cgacagtagc tcgtggtcta
     7501 tttgaaacag ataaggacct ccgcgaccaa gcttatcgtt taatggggct ataaaaagaa
     7561 tccgtttcaa gcggattttt ctagaaagga atcattatgg atcaactgca gattaaggat
     7621 ttggaaatgt ttgcctatca tggtcttttt cctagtgaga aagaattggg gcagaaattt
     7681 gtcgtttcag ccatcctatc ctatgatatg accaaggcag ctacagactt ggatttaaca
     7741 gcctctgtcc attacggaga attgtgtcag cagtggacga cttggtttca ggaaacgagt
     7801 gaagatttga ttgaaacggt agcctataaa ctggtggaac gtacctttga gtcttatcct
     7861 cttgtccaag aaatgaagtt ggaactgaaa aaaccttggg caccggtgca tttgtcacta
     7921 gatacttgct cggtaaccat tcatcgccgc aagcaacgag cctttatcgc cctaggaagc
     7981 aatatgggag ataaacaagc aaacttgaag caagccattg acaaactgcg agctcgtggc
     8041 atctatattc tcaaagagtc cagtgtctta gcgacggagc cttggggtgg agtggagcag
     8101 gatagctttg ccaatcaagt ggttgaggtg gaaacctggc taccagcaca agacttgtta
     8161 gaaaccttgt tagccattga gtcagagctg ggacgggtga gagaagtgca ttggggacct
     8221 cgtttgattg atttggactt gctctttgtg gaggaccaga tcctttatac agacgacctc
     8281 atattgcctc atccttacat agcggaacgc ctttttgtcc ttgagtcctt acaggaaatt
     8341 gcgcctcatt ttatccatcc gatattaaaa caaccgatcc gtaacttgta tgatgctttg
     8401 aaaaaataga aaaactctag ttttcagtta cttgcaactg aaggctagag tttttaaaat
     8461 aggtcatttt cttctgggag gaggatagtt tctctaccgt ccatgtctaa aaccagtact
     8521 cttgggggat aacgagggtc gaaaggatgg ttaaagtcaa aatcaatggc tgtagggagg
     8581 tgttgacttg aaaagtggaa ggtaatcttt ccttggttat taagcaattg aaactcgagt
     8641 tcttcttcca attcaaagac attttttaag aaatggtcga taatatacca aaaagagtca
     8701 atgatgtcat caggcaagct ggtaacaatg ccaaaactag cagaccgcat gtgggtattg
     8761 gtaaaagcca tatctctgtc tcctttcttt tcccttatca tacagcaaat aggattaaaa
     8821 atcaagaaaa ggtgattttt tgaaaaggat tttaactaca gggaaaaaga aggaaaaaaa
     8881 ttcccaaaaa tctaccgaag ttaataggta aattcccaaa ttaacttgat tatataactt
     8941 tcagttattt tgagaagtta ccgaaaaata ttttttatat ctattgactt ttaggggtga
     9001 aatttggtat gatagtaggc ggtattgttt accccatttg aaaggccccg gaaccttcca
     9061 aatacttttc gatgggaagg aacacccatc accgtaaaca aaaatcgaac tatatatagg
     9121 agaaatcatg aacaaaacaa catttatggc taaaccaggc caagttgaac gtaaatggta
     9181 cgtagttgac gcaactgatg taccacttgg acgtctttct gcagtagttg ctagcgtact
     9241 tcgcggaaaa aacaaaccaa catttacacc acacactgat acaggtgact tcgtgattgt
     9301 tatcaatgct gaaaaagtta aattgactgg taaaaaagca actgataaaa tctactacac
     9361 tcactcaaac cacccaggtg gattgaaaca aatctctgca ggtgaacttc gttctaaaaa
     9421 tgcagtacgt ttgattgaga aatcagttaa aggtatgctt ccacacaata cacttggacg
     9481 cgctcaaggt atgaagttga aagtatttgt tggagctgag cacactcacg ctgcacaaca
     9541 accagaagtt cttgacattt caggacttat ctaaggaaag gaacaataaa gtatgtcaca
     9601 agcacaatat gcaggtactg gacgtcgtaa aaacgctgtt gcacgcgttc gccttgttcc
     9661 aggaactggt aaaatcactg ttaacaaaaa agatgttgaa gagtacatcc cacacgctga
     9721 ccttcgtctt gtcatcaacc aaccattcgc agttacttca actgtaggtt catacgacgt
     9781 tttcgttaac gttataggtg gtggatacgc tggtcaatca ggagctatcc gtcacggtat
     9841 cgctcgtgcc cttcttcaag tagacccaga cttccgcgat tcattgaaac gcgcaggact
     9901 tcttacacgt gactcacgta aagttgaacg taagaaacca ggtcttaaga aagctcgtaa
     9961 agcatcacaa tttagtaaac gttaattcga aagaattact atacttatac agagcacctt
    10021 tcggggtgtt ctttttttat actttcttac taaattggtg caattgacac agttgttgcg
    10081 actttagtcg cttacaaatg tggctgcaac ctgacaaggt cagttgcctc aaaacgttaa
    10141 tcaatacgat tatatcaacg tttcaaagca ctttgcaaag attttgcaaa gtgctttttt
    10201 gtcgattttt gagctcgaat agctttgtga taaccttctg ataacctctt ttagatttta
    10261 aacatcatga ttttactttg ctttattgag ataatctgca aatttgtcaa tagagttttt
    10321 ctcaccagtt tttgtggtgt gagtatagac atctaatgtc atctgactgc ttgcgtgacc
    10381 gagtcgtttt gatgtattag aaggatcgat tccaatttca gacataagtg ttgcatgtgt
    10441 gtgtctgaac ccatgtgggc taattggttt taatttatgc tttttaataa ttcgattcag
    10501 tacgttatta atgtaatcag catgcaaagg ttcaattttg tcattacttg atatgtaata
    10561 gaacatatat tcgttactaa tattcaactt tgtaagagga aactgagatt gttctttcag
    10621 atagaaattt ctccattgct taataatatc gattgtctca tcatcaagat agattgtacg
    10681 aactgaagat tcattctttg tgctcttttc aatcgcttta ccgtcaattc ttcctagact
    10741 tttatctatg ttcaatagat tgctgtcaaa gttaatatca gcccatttta aagcgtataa
    10801 ctcccctttt cgtagtccac taaatgcaag cagtctaaat aaagcaaaat gtttaaatgg
    10861 ctcagtttct ttcactatat caaggaaatg atgtaattcc tccagagacc aaaaattatc
    10921 tgatctagtt tttttgattt taggcatgat aacattcttc attggatttc gatcaatgta
    10981 gttcatgttg attgcaaaat caaaaatttt tgaggtatag gattttatct gtttgatatt
    11041 tttgaaagtc tgagattttt gtgtaataaa gttttgacag tctaaaggac tgatttggtc
    11101 aataaattta ccgccaaata ttggtagaat atgtattcga tagatattct tagtttggga
    11161 agaagttgtt tcttcaacgg taccaacata tgccttaaac cattcttgat aaaattcttt
    11221 ataagtaact ttgggattag gtttgtcggt atttactgta ggggcgacga caccagcttc
    11281 taaacgagct tcgtacaatt ttgcctcttt tttggttttg aatcctctct ttaagtgcct
    11341 ttttttcttt ccataagcat ctaagccaat gtaaaagctg acatagtaaa ggactccttt
    11401 ttttgttttg tatttgtgta tagacatttt ttctccgtat ctaagttttc ggagcttatg
    11461 taatcgagaa atgagtgtct atctattttt tctatcttct tggcggatag cgtattcgtc
    11521 tgttttaatc cagctctgcc aaactttaga atctttattt tggtgattca tatagtctat
    11581 aaactcagac ataaaacgtt tgtttttctc agctaggtat tctgagaata tatgacgaaa
    11641 ttcatctgaa aaactatcat caagtggtag tgttaatgcc ctttcgccaa attcgatact
    11701 tttctttaac ttctccatat cgcttttaaa aggtgtcaca gaaccaattt tagatgtttt
    11761 gaaactatct gtaacaattt taagatttga tggagcgtgt gggttgctat caagtcccat
    11821 aatataagca acggaaacat taaagtattt tgctaatttt tcagcattat cttttttagg
    11881 tttgcggatc ccctgattgt atccagaaag tgtaggatag gctatatctg tagcttcgct
    11941 aatttctgtc aatttttttc cgctatcttc tattagttta gtaatatgat tgctcatgag
    12001 aacctccttt ttatcattat aacacatttt attctttttg aataaaaaag cttgacaaaa
    12061 attcaaaaag aatatataat tcaaaatgaa taacaaatta aatggtttcg gagcttattt
    12121 aatttagaaa tgaggtgtta atttatgtcg gaaactactt tagttgtcca gcttcctaaa
    12181 gaattagaga agcaattacg tgctcattat gatgagatga tttcaaatgc tgtggcaaga
    12241 gtatttgaag ataaagaact ttataaaccc atggttcgta tgtcaggttt gtctaggtgg
    12301 ttagatgtat ctacgacgac aatccaaaaa tggacaaaag aaggaatgcc aaccatggta
    12361 attgatggag tgactttata cgataaaaga gcagtaacaa gatggttgaa gcaatatgaa
    12421 agataggtac ttgtatgaat ggtgttcagt ggatcaagat aacaacagat atatttgatg
    12481 atgaaaaaat tcagctaata gaatcgatgc cagaaggaga tactcttatt gtaatatggt
    12541 ttaaacttct ggtgcttgca ggaaagcaaa ataatagtgg aatattgagt ctaggaaata
    12601 aggtatatta cacagaagag atgctttcaa cagtgtttag acgaaaagct acatcggtaa
    12661 aattagctct cagcatgttt gaagaatttg gcatgataga aatcatagat ggcgctatta
    12721 ccattccaaa atgggagaaa catcagaata tagatggcct agaaaagatt agaaaacaaa
    12781 caagggaacg tgttgctcgc catagacaaa aacaaaaagc actaattaaa ggcgagccac
    12841 aactcatttt agactgtaac gttacagtaa cggatgaaaa tgagaatgta acgcaagaga
    12901 ttaagaacga gagtaagaat aagaaagaga ttgagaatat actggataat agttctagaa
    12961 agcctgaaac ttatattcca cctaaatatt attctttgtt agaatccatt tcttataaat
    13021 acaatgatag atttttgtat cctaacaatt acactttaac acatgctcag aagatgaaga
    13081 taggtgagta tttagctagt ggatatgtaa ctagtgatga agtcattagc atgattgaac
    13141 ggattcctga ggatgcagct tctcctttgg cgtatttatt taagtctatg gaaaatttaa
    13201 agcaggaacg gatgttggag tgcaaaacaa ttgctcatga aaatgccaga aagaagtata
    13261 tgataaatga gtagatatcg gactattcta aagaaatttt atattactga agaacaaaat
    13321 gaaatagcga ataacttaat taaaatgaca aaccatctaa gtttttcctc ttatgctaga
    13381 aaaatgttgt ttaaacgttc gcctatttat cttcaatttg attttaaatc ttatcatgac
    13441 tttatatttc aagtaagaag gattatcaat aacttacgtc agttagaaag aatagcgaaa
    13501 caaagtgaag atttagataa tgtaaggata tttcactgct gtgttgagat gatgatagga
    13561 tatgaaaaga agacaagcaa acaagtgaaa gaacttgtga aacgactaaa taagaaaacg
    13621 aggtgataaa attatgagaa taaaatcggt gttgaaacag gtatttttga cagaagaaga
    13681 aaataaaaaa ttaaatgatt gtatgaggaa ggaaaatatt tgtaattttt ctgagtttgc
    13741 tagaaaaaaa ttaatccgaa cagatttgaa cattcacaaa gtctcttttg aaggtttagt
    13801 tcctttaaca gaagagttgg aacaggtagg aaagaatatc aattctatag cccgtcttgc
    13861 aactgtagtt gggagaataa gctatgaaaa taaaatggat atgtctatta tgatgcaaaa
    13921 aatagttgac gtgatggagg aaaaagatgt ttattttcaa aagtagtatg gataaggatt
    13981 ttgatcagat atgcgaatac atattgaaat taaagcgagc agaattattt cttcatggga
    14041 ttaaacaaca agataaaacg aaggctcgtt tgtatcatgc gatgctggaa tattacgatg
    14101 ctaaccataa aaaagtaaga catatgagaa ttttaaaata aggaaacgct gttaaaaatt
    14161 tgcagcgttt tttatatatc ttcttaaaat atttgtacac acaaatgaca gtcaaattga
    14221 tttttgggat tattttatcg agaaggaaag atttatcata atatgacttt gcgagctcag
    14281 agtctgtata cacatagtgt atgtactttt tgggatgatt tgaattttgg ggaatcccca
    14341 aacccctgat gaattatacg agcaaaaaaa tttgggagta agttttagaa agaactattc
    14401 tacagtattt aaaatctgac tttaagtaaa ttttttatta tgtgactatt agtctgtctc
    14461 actccgctag gtgcgagaca aaaaaacacc cgctatgcgg gatgcttttt ctgttcaccc
    14521 caaaattcac cttattgttt tagagacttt aaagcaaatt cgcctactgt cataggaact
    14581 acattagaag tatttacata aatctgtgtt gttcctgtgt cactgtgtgt tagagcctca
    14641 gaaatagctt ctaagttcat tcctgcttgt ttagcagtat gtcgcagttc atgaggtgta
    14701 gcatgtgcca gctctttatg ttgctttctg atacttttca ttttattgtt tagatagtca
    14761 gcatgtaaag gcttattgat gtttcctcta gtgtctatat aagtaaagac aaaccgtcca
    14821 gatagtatgt cttgcatgct gtttacttct tgtctcttgt tttaggtttt gagataatag
    14881 cgacaacaga agtagttgtg gactattttt atgaatgttt aaattaatca ttagaaaatt
    14941 gttggcagtt gagagctcta agtaaatcat tttgatacaa tttatggtcg cacatagata
    15001 gtatgtcaca aaagaaactg cggctcgtta aaaatttcat tttttcagag ataggtctct
    15061 ttttacatga tttacctatt aatattagta aatgttaaag ataagtattc tttttatttt
    15121 ttggcgaatg ccggcctttt tgccacaaat gagtatcaac atctttcgaa tggaatagtt
    15181 gacaactggt tttaagaatt atactattga attgaataga taaggagttg atttgatgca
    15241 agagacaatg attgattaca aacaaaaaat ttaccaagag ctatctcgct taacaaaggg
    15301 gctaggaagt gagaagcgtc ttgatatttt aaacatatta tcacagggac caaagacagt
    15361 tgagggaatt gcgcatacga cgggtctctc tgttgccaat acttcgcgcc accttcagat
    15421 tttaaaagaa agtcacttgg tggtgacgag tcgaaatggt aattttatcc gttatagctt
    15481 ggcttctgaa aaggttgtcc agttagtcct tctgttattt gccgtcggtg aagaacagtt
    15541 agcagaagtc agagcgattg agtcagacta tgatgaggca gctggtgttc ctcagattga
    15601 gctcgctaat gcaattgaat tagcagctaa gacagatgct ctcctacttg atttaaggcc
    15661 tgttgaggag tatgaggcag ggcacttgcc aaaagctctt aatttaccaa tcgatgagtt
    15721 tgatcaaaaa aaagctcagc ttcccaaaga gaagaccatc attgtttatt gtcgcgggcg
    15781 ccaatgtggc tatgctaacc tagcagcaca ggatttacag agtttgggct acagtatcta
    15841 tagtctcaat cgcagttttg ctgattggaa gtggggcgca tcctctcgtt cagaacttga
    15901 ctaaggtgaa ttccttagtc ttttcttata accggagact cgtgagcaaa atcattgtaa
    15961 aatagattta gtatgctata attcaattat tcaataaaaa agttgaattc ttttttaccc
    16021 aacctattca acaattttat tgaattatat ctttgaaata cttcttattt agtatctcaa
    16081 agaaactaag tgatacaaat tggattttta gaagggctaa gttaaccttt ttgaaattca
    16141 ttcttttatt cttatgctat tcaattatat gattgaatag aagcgattta ttttagatag
    16201 gagtttttta tgacgacaca actaacaacc gaaacgtttg cgaaagctat ccaagatggg
    16261 gtgagtctta tcgattttgg ggcgcaatgg tgcccacctt gtcggatgat ggaaccggta
    16321 gtggaagaac tttcaaagga ctttgacgga aaagcaacga ttgcttaggt ggatgtggac
    16381 caatcacaag atctggccaa tctctttggc attcgttcta tcccaacaat ggtgattttt
    16441 aaagatggca aacccgttga tgctcttgtt ggtgttcatc ctaaacaaac attgatggaa
    16501 aaaatccaag cacagttatc atgaggtgat ttatgtatga tacaattatt gtaggttcag
    16561 gacctgcagg gtacacagct ggcatttatt taagtcgagc tggtttgaaa aatcgattga
    16621 tcacaggcta ttctgagggt ggtcaattaa ccaccacaac cctcgtagaa aattatccag
    16681 gttttgaaaa cgggattgaa ggcaataaat tagtccaaac aatgcgccgc caagcagaac
    16741 agtttggcac cgaaatgact tttggatttg ttcaaaaaat tgaaggcgaa tcgtctcctt
    16801 tcaaggtaca tctggataat ggtgaaatca ttgataccaa ggccgttatt atcgcaacag
    16861 gctctagcgc taaataccta gggattgaag gcgaagaaga ggctgtcgga aaaggtgtca
    16921 gcgcttgtgc gacctgtgat gctttcttct ataaagagcg tgaagtcctt gtagttggcg
    16981 ggggtgatgt cgctatggaa gaagccctct atttgacacg ctttgcatcg aaagtgaccg
    17041 ttgtccaccg ccgcaatgag ttacgcgcat ctgatattat ggtcaaacgg gctcaggcaa
    17101 atgataaaat tcattggatg ttaaattata cgcctgtcaa agttgcatct aatctgatgg
    17161 gaatgactgg tttggatgtt cgtaataatg aaacaggtga tattgagcac attcaggctg
    17221 acggtctctt tgtggctatt ggtcatcagc caaacaccga ttttcttgat gggaaattag
    17281 cattggatga taaaggttat attcaaacac aggcaggaac agcaaagacc tctgttgcag
    17341 gtatttttgc agctggtgat gtgcaagatc caaaatatca acaagccatt actgcagcgg
    17401 ggagcggtgc tattgctgcc ctagatacat tggcctatat tgataaccta tgatggattc
    17461 agtcacgtct agtatcgaat tagaagaact gttagacgaa catcaaaatc tagctcttct
    17521 tgtttcagga aatcaatgca gtctatcgtc tcttggacaa aaagaactca tagacacggc
    17581 tgaacgagag catttggtgc tttaccaagc agatattttt caagtgcccc gtctcatttc
    17641 tttatttggc attcagccta ccctgacgag tttattagta tttaaaaagg gtcaattggt
    17701 tgataggaaa gaggtttaaa tggcaagaaa agctttgata caaaggaaaa agaaggccta
    17761 cccggttcaa gaatactcac gctgtttggc atgcggccgc ccgcattcgg tctaccgcaa
    17821 gtttaaactt tgtcggattt gtttacgcca gcaagcttac caaggtgtca ttcctgggtt
    17881 gaaaaaagct tcttggtagt ggaaagaaag gaaggaaaaa atatggaaat tagttactgg
    17941 tcagatattg cctgtccatt ttgttacatt ggtgctagtc ggatgaagcg tgctatggca
    18001 gctgttggtt taaacccgga agatttgaca atgaaagctt accagctaaa tcccaatgcg
    18061 cctttagaaa caagtgaaac catgctcaca gagtttgcta ccagtcacgg tatgaccact
    18121 gagcaagtta aagctcagtt tgcgcacatg gaaagcatgg gaaatgaaga agggcttcgt
    18181 attgatatgg cggggattat cccaaccaac actttttcag ctcaccgtct tattaaatgg
    18241 tcgcagaagt atttagataa aaaagatcat caaaatttca ttacggcctt gtactatctt
    18301 tactttgaag aacatgccaa tattgcggat cattctgtgt tactagctgt tatcagtgag
    18361 ttcgatctac cacaagaaga ggcagcgcag gttcttgctg gagatgcgtt tgctgacgag
    18421 gtcaaacgcg atatcttaga agcccaccaa tcaggcgttc aaggtgcacc tttctttgtt
    18481 ctgaacaata agtatggcat ttcaggtgcc cagccttatg aatacatgtt agcgacatta
    18541 aaaaaaattc aagcagagga aggagcacaa taaaatgata gatacccctt ttcaactact
    18601 agacagcaat tcagaagaga caggtttagt ttgtgggatt gatggctgta gcatagatcc
    18661 ctcaacacag ccgttagaaa atacctatca gccattagaa aaaagtaaaa aagcagtcat
    18721 tgggaggaca gaatggaaaa acaaagagta gaagccttgt cagatgctat attggccatt
    18781 attgttacaa ttatggcttt ggagttacag ttacctgagg aattgactgt agcaggactt
    18841 agaagcatgc tacctatgct ttttatctac attactagtt ttttgcaaat catggcagtc
    18901 tggctatatt atcatgaact ctacaaatta gtagatcatg tcagctttca tttatttgga
    18961 gccaatagtt tttggctttt aacagctagt tttgttccct tagcgactcg agggattgga
    19021 cagaattccg ctaattttgc ctttctcctg ttttacctca tcgttcttgc cctttgggat
    19081 gtatcttgga taatcatgac agcaatggtg atcaagacga gtcaaagaca gttatcagct
    19141 gaagaaaaaa agatacctta tcggtgggca ctattatatg gattgcagct agtgatcata
    19201 ttattagttg gttatctttt tccaaatttc attccttttg ctccgctact gatgattatt
    19261 aatgttatct atggtttttt aagcaacaaa tcttgaaaaa tactaaaaat aatggctaac
    19321 tagcatcggt tataaaaact aggtcttaag aaagctcgta aagcatcaca atttagtaaa
    19381 cgttaaaact tatttatatt tattgaaagc atttcgtatc aaaaatacgg gatgcttttt
    19441 ctgttcaccc caaatatttg aaccttgaac ttccgtgaat aggttccatt acgactatta
    19501 ccagaattat agactacttt atcgtaaggt tcatactcta aaagggctga taattctgct
    19561 ttaagcaggt cgttcatagc tgtttcaaga gcagaacgaa aaaattcatt gatatctcgt
    19621 tttttgacta ggaagttaag aaattctgtg gtaaactgtg tcatagaagt aaatctcttt
    19681 ctagtaatgt ttcgcaactc tactataacg gatttattcc tttttgtgtt tacacaactt
    19741 attttacact acctggtcga ataacattag aaacgaatgt cttttatgtt cgaggtatag
    19801 cacaatagtg ggaatacagg tagtattgta ctgagaggta tcttatgttt agtaaactaa
    19861 gtgaccatga ctttaaaaaa ggagtcttta gaacaaagtt cagtcaatta gtaactccat
    19921 taggaaaaga atctgactgg gctcattcaa aaggtccaga atatctttgg attggattag
    19981 ctctgcaata tggaaatcga atagagcaaa tggaaagagt gatgttagct ctgactaatt
    20041 tatcaaaagc attagattta gaaaaaatct tgcctttacc ggcaatgagt ttaatactta
    20101 atttagatat cgatgagaag aaaatttttg ttgaatcact aaattctgct ttcgacttat
    20161 caatattttc tccgctttca ataattttgc ctgatagcga agaggttttg agtaaaaatt
    20221 tttacagcaa gagtcactct tttaatcaaa gactagatat acttgttaaa gtactaaatg
    20281 aagtatctga ccaatatagt caattatcga cggatgtaag atatttttta ctgtattaca
    20341 agatgttaca gggaaaaatt caatttgtag aatcgcagtt acacatggta gacggattaa
    20401 gtaagtaccc ttacttagat atatcagatc cagaaatgag aattattcgt cctcagattc
    20461 gctcaatgga ggtagcttta tcaatgtccg aagatatgaa ttatccttat agtaaaagat
    20521 tttggaataa tattagccag ctaactgatt gtgaggtata ttcaattgtt atggatagaa
    20581 gtaatactat agatttgaat aagataaaag atacagtatt ttcgacatta aaatattaca
    20641 gagatatgct tcaaagtata gagcctttta acgagaagct ctatgttttg actagtatcc
    20701 taacctattc ttataagcgg ttactagaat taatgaatca tggtttgcaa tatacgattt
    20761 caggtcgttc catagtaagg tcgtgcattg aaaactatgt gatgacaaaa tatttaattg
    20821 ccgaagaaga aaaacacaaa aatatttggg aagaatatca atattatggt attggtggat
    20881 ataaactgat ttcagaacgc tatatagaaa atcaacctga attaacaaac tctcatatta
    20941 attttgaata tctgaattta ctagtatctg aatttcaaaa taaagaattt atagatatgg
    21001 atactagata tttcggaaaa gggaatataa gacaaaagtt tgaacaagtt gaggaagatg
    21061 atttatataa atatcactat gattatgatt ctcaatttga acatggactt tggggagcga
    21121 ttagagaaag ctcaattttg aaatgcaact ctgctggaca ccaattccac ggtattcccg
    21181 atatagacga tattcaaaaa ttacccgatg ttagtcatga tctggtgatg gttttaacaa
    21241 agcatctatc agtgattaaa gaagtgtttc ctattccaag tgagcaaata gaaggtgata
    21301 gtttatgcta gatagactaa gacaatcaca aaatgaatat ttagagttac tgaagagagt
    21361 tagatgggac ttggacttag agaatgtagc ttatcattta gataaaatta gaaatttttg
    21421 gtttagaaaa caaaaattat tagagatgtg tagccaatat ctctttaata attcgaacac
    21481 atatttttat a
//
LOCUS       DAVKWZ010000034        20301 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000034 DAVKWZ010000000
VERSION     DAVKWZ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20301
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.034"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            124..288
                     /locus_tag="VW854_001322"
     CDS             124..288
                     /locus_tag="VW854_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV0262551.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            372..938
                     /gene="amaP"
                     /locus_tag="VW854_001323"
     CDS             372..938
                     /gene="amaP"
                     /locus_tag="VW854_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEV0262552.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            950..1120
                     /locus_tag="VW854_001324"
     CDS             950..1120
                     /locus_tag="VW854_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEV0262553.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            1159..1767
                     /locus_tag="VW854_001325"
     CDS             1159..1767
                     /locus_tag="VW854_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV0262554.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            1798..2001
                     /locus_tag="VW854_001326"
     CDS             1798..2001
                     /locus_tag="VW854_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV0262555.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(2111..2383)
                     /locus_tag="VW854_001327"
                     /pseudo
     CDS             complement(2111..2383)
                     /locus_tag="VW854_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2587..2874
                     /locus_tag="VW854_001328"
     CDS             2587..2874
                     /locus_tag="VW854_001328"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV0262556.1"
                     /translation="MIVSLKTKSRKAKDMAESIQGWLAQFLVNLFKSITFDCGKEFSK
                     WKDISNHHDSESFFANLGCPRQRCLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            complement(3152..3922)
                     /locus_tag="VW854_001329"
     CDS             complement(3152..3922)
                     /locus_tag="VW854_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV0262557.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTTKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(3937..4775)
                     /locus_tag="VW854_001330"
                     /pseudo
     CDS             complement(3937..4775)
                     /locus_tag="VW854_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
     gene            complement(4772..6247)
                     /locus_tag="VW854_001331"
     CDS             complement(4772..6247)
                     /locus_tag="VW854_001331"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEV0262558.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKTYGSQFDYASDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTALKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
     gene            complement(6571..7926)
                     /locus_tag="VW854_001332"
     CDS             complement(6571..7926)
                     /locus_tag="VW854_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV0262559.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGCFSTAFDWRAIILSVVCLIISVLTYFPFVKMADKTEL
                     S"
     gene            complement(7936..8253)
                     /locus_tag="VW854_001333"
     CDS             complement(7936..8253)
                     /locus_tag="VW854_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV0262560.1"
                     /translation="MDESNLESVMGLIMYGGDAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTKEAQGEETSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            complement(8270..8587)
                     /locus_tag="VW854_001334"
     CDS             complement(8270..8587)
                     /locus_tag="VW854_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV0262561.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(8602..9498)
                     /locus_tag="VW854_001335"
     CDS             complement(8602..9498)
                     /locus_tag="VW854_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEV0262562.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLNRIEELGFDFVCLTGNPGTGVSNREIIKAVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(9501..10763)
                     /locus_tag="VW854_001336"
     CDS             complement(9501..10763)
                     /locus_tag="VW854_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV0262563.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFITGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDVLLYLPFVIAIEKRFKLLED"
     gene            11043..12221
                     /locus_tag="VW854_001337"
     CDS             11043..12221
                     /locus_tag="VW854_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEV0262564.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDDYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDSNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
     gene            complement(12449..12895)
                     /locus_tag="VW854_001338"
     CDS             complement(12449..12895)
                     /locus_tag="VW854_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0262565.1"
                     /translation="MTIRFEEKVSIENAQFVCQWSNSLGKSFQEQWMGPRIPFLLTLQ
                     ALEEVFSIFDEQEFVGLIQKIRLKDSNLHIGRFFINPQKQGQGLGSQTLRKFVSLAFE
                     NEDIDNISLNVFEANQRAQNLYQKEGFEIVQMVEAPVRKYSGLKLE"
     gene            12965..13624
                     /locus_tag="VW854_001339"
     CDS             12965..13624
                     /locus_tag="VW854_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEV0262566.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELGLGLLFLLYSWELLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTFHLILIASSGWNLVMVSFLALGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLKKKKERLPFVPFLLLATCLIIFGKLLL
                     V"
     gene            complement(13596..14372)
                     /locus_tag="VW854_001340"
     CDS             complement(13596..14372)
                     /locus_tag="VW854_001340"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEV0262567.1"
                     /translation="MPKTLTEKLNAIKAAGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDITLIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIRQAVAYQK"
     gene            complement(14365..15588)
                     /gene="trpB"
                     /locus_tag="VW854_001341"
     CDS             complement(14365..15588)
                     /gene="trpB"
                     /locus_tag="VW854_001341"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEV0262568.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGLGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            complement(15566..16165)
                     /locus_tag="VW854_001342"
     CDS             complement(15566..16165)
                     /locus_tag="VW854_001342"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEV0262569.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGEGHVPNSQADYLLFDAPVAGSGQPFDWGQLDTTGLPQSFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKNGISRTK"
     gene            complement(16152..16919)
                     /gene="trpC"
                     /locus_tag="VW854_001343"
     CDS             complement(16152..16919)
                     /gene="trpC"
                     /locus_tag="VW854_001343"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEV0262570.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            complement(16916..17920)
                     /gene="trpD"
                     /locus_tag="VW854_001344"
     CDS             complement(16916..17920)
                     /gene="trpD"
                     /locus_tag="VW854_001344"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEV0262571.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            complement(17931..18497)
                     /locus_tag="VW854_001345"
     CDS             complement(17931..18497)
                     /locus_tag="VW854_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEV0262572.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEEGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            complement(18494..19855)
                     /gene="trpE"
                     /locus_tag="VW854_001346"
     CDS             complement(18494..19855)
                     /gene="trpE"
                     /locus_tag="VW854_001346"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEV0262573.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKTLATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMKVELFRY
                     VMHLTSVVKGRLLPELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
BASE COUNT         6330 a         4409 c         3469 g         6093 t
ORIGIN      
        1 ttaatcaaag aatttgattt tttgatatta aaataaaaaa ggagggtagg cagtgttgtg
       61 atcaattatt gctggaggtc ttattggtct cttgacaggt aaaatcacta aaaaagtagt
      121 tctatgggaa tcatcgcaaa tgtattcgct ggtttagtcg gggcatatgc aggacaatct
      181 cttttaggta gttggggtcc atcaatcgct ggaatggctt tgctcccatc tattgtaggt
      241 gcagcgattg tgattactgt agtgtcattc tttacaggta gaaagtaaac ttttcgccag
      301 taaagttagc aaactatttt taaatcaatg acgggaaaaa tagtttaaat gttaaatcga
      361 aaggattgta tatgtcaaaa gcaaagaaaa tatgtttcat tattttctgt attttaatct
      421 tgacaatttt ccttcctgtt ttgatagatt atcatcaagt tagtgatcta ggtattcatc
      481 tacttagctg gagacagaac tccgtagttg aattctatct tgctagatat gtcttttggg
      541 ggacagtggt tctatcaact ttagttttat tatccatttt agttgtgatg ttttatccta
      601 aacgttactt ggaaatccaa cttgaaacta aaaacgatac attaaaatta aagaattcgg
      661 caatcgaagg ttttgttaga agtttggtga gtgatcatag attgatcaag aacccaactg
      721 ttcatgtaaa tttacgaaaa aataaatgtt tcgttcatgt agaaggtaaa attcttcctt
      781 cagacaacat cgctgacaga tgccaaataa ttcaaaatga aataactaat ggattgaagc
      841 agttttttgg tattgagcgt caagtaaaac ttgaagttgc agtaaaaaat taccaaccaa
      901 aacctcaaaa caaaaagact gttagtcgtg tgaagtaagg aagtaaaaaa tggaatggct
      961 taaacaatat cgatatccaa ttatcgctgg tctcataggc gtatttctgg cttgtttgat
     1021 tgtctccttt ggcttcttca aaacaatatt tgtattgatt ttaggagcac tgggagttgc
     1081 agctggatta tatatcgaaa aaaactatat agataaataa aaaaataaaa attactaatt
     1141 taattaaagg agtttcatat gtcaaacgaa aaaaacacaa atactaacgt agaaaagaaa
     1201 gatgctactg ttgtagctca cgaaatcaaa ggggaactta cttacgaaga taaagttatc
     1261 caaaaaatca ttggtctttc actagaaaac gtttcaggtc ttttgggaat cgatggtggt
     1321 ttcttctcaa atcttaaaga aaaaatcgtt aacagcgatg acgtaacaag tggtgttaac
     1381 gtagaagttg gtaaaacaca agttgcagtt gacttaaacg ttattgttga gtaccaaaaa
     1441 aatgttccag ctttatattc agaaatcaga gaaatcgtat cttcagaagt tgctaaaatg
     1501 actgacttgg aaattgttga aatcaacgta aacgttgtcg acatcaaaac taaagaacag
     1561 catgaagcag actcagtaag ccttcaagat cgcgtatctg acgttgctga atcaacagga
     1621 gaattcactt cagaacaatt cgaaaaagct aaatctggtc ttggatctgg tttctcaact
     1681 gttcaagaaa aagttagcga aggtgtagaa gctgttaaag gggcagcaaa tggtgtagta
     1741 tctcacgaaa acactcgtgt aaactaagat aaaataaata taacaggaga aattatcatg
     1801 tcagtagaag aaaaattaaa tcaagctaaa ggttctatta aagaaggtgt tggaaaagcc
     1861 atcggtgatg aaaaaatgga aaaagaagga gcggctgaaa aagttgtttc taaagttaaa
     1921 gaagttgccg aagacgctaa agacgctgta gaaggtgctg tagaaggtgt taaaaacatg
     1981 ttgagtggcg acgataaata aggttaaaag ttactttatc tttttggtaa cattagtcaa
     2041 aagagtctga gtcgagatga ttctcagaaa acaaaaagct agagattccc aattgcggaa
     2101 ctctagcttt ttaattttgc ctctttctct tattatattt cagcaggttg ttggccatga
     2161 gtatgaatcc catgtcaatt ctcacttgac gcttacctct cagatgacat ctcttataac
     2221 ccaaacaaac ctttatctgc ccaaagacag atttcatatc aatcttacgt ttagcgaaaa
     2281 tttgtctacc cttggaagat aaaagtgcct gatattcttt agtttttaaa cactggtaac
     2341 gttcattcat atacagtctc ttttgagggg ctgattcagg ttcataatcg cagtcaacat
     2401 tgatttcaag gctgtttgct ttctatctcc cctttttcat gatatatttt cgtacaggtg
     2461 cttcaatagt ttctatagta aaatgaacca aaaatagtac acgttgcggt ataatgttct
     2521 tatggcatat tcattagatt ttcgtaaaaa gttctctcct atttgtgagc gaacaggtat
     2581 agtcaattga ttgtcagttt gaagaccaag agtcgtaagg caaaggatat ggcagaatcc
     2641 atccaagggt ggctggcaca atttctagtg aacctcttta aatctattac gtttgactgt
     2701 ggaaaagagt tttcaaaatg gaaagatatt tccaatcatc acgatagtga gagctttttt
     2761 gcgaatctag gatgtccaag acagcggtgt ctcaacgaac attcaaatag acttttaagg
     2821 tgtcatgact tgccaaagca gacagatttc aacgaagtca gtcaagaatt ttaggctggg
     2881 taaaaactca atttcaggag aaaatgaaat aaatattccc acaataaaat gcatagtatc
     2941 aaagtttttc caacacctga tattatgcat tttttaattt taaagacttt ttcccagcct
     3001 caagaaatca catcccacct aaatcctttg gataccaaat acctcatcag gtattttctg
     3061 agttggtaag actgtctagc ttaatttgac aaatggaggg cgttaaatcc agtttttaca
     3121 ggaagtctat ttaaatattt tcacactgac cttacaaaat atgaaataaa ttatataaag
     3181 tcattcccat gacaaagaat aacatccctt taaaacagta tcttacccaa ttttcaggta
     3241 agacttttga tacaaccgtc cccaataccc ctccgcatat agaagcaaga aaaataactg
     3301 gaacaggtgc taaatgatac tgatttaaac ctgtaaccac aacaattgta gccaaatgag
     3361 acaactggga aaagaaaata attgcaagag aatacatcgt tgcttctttt atcgaaatag
     3421 aaaaaaacac catcaataaa gatacattga gcggaccacc accaatccct aaaaaactag
     3481 aaatcaagcc caatagaaga ccacaaatca tatagaatcc tatcttatca aattgataag
     3541 tttttttaaa atctgttaaa acaaatgata caagtagcat cacaaatagt aaaatcattt
     3601 gaacaatgga tactagccgt actgataatt gagataacaa aacttgaaaa ataagatgac
     3661 ctagaaaccc tcctaaaaca gaagaaaagg ataccgtaaa aatcaatctc cattttatct
     3721 ctttactttg tgaaaatctt ttggttgttg aaataatcgc cattataaaa acagagaaac
     3781 tagaataaaa agcaatatca ctgaccgaat ggtatccaaa actatccata agtggcttaa
     3841 ttataattcc accacccatg cctgatatag aacccaatcc actagctatt ataatgatta
     3901 gaaaataaat aatctcaacc actttttaag tccatcctat tctttcaaac atctaaaacc
     3961 tagatggcta ctagtggaag tagaaataca tccatttcta gcagctactc ggtatcgatt
     4021 acaataagaa caatgacata aaaaggaacc tcctctgata gcatactcct catcttgtat
     4081 cccatatttt ggtaactcat agtcatcttc attaaaacta gctaataagg tatatctcgg
     4141 attgcgacac cattcccaat catttgatat agaccaaaat cattaggtaa aaattcatag
     4201 acaggagccg ttcctataaa tccatctaag gcagtatttt cataaggaaa tcttccttgc
     4261 caagtattgg catgataata tccaccttct aaaagagtat ctccccaagg atattcagat
     4321 tgtcttcctc ctctagcagc atattcccac tgtgcttcag tcggtaaaga cattccgctc
     4381 caattacaga aagccaatgc atcttctaaa gcaacatgaa ctactggatg gtcttctaaa
     4441 cccactagat tactgttctc accgtaggga tgtttccagc aagcgttcga aacttgtaac
     4501 caccacggag ccccagcagg atgaggatat ccctcccttt ctgcctccgg aacgaacaat
     4561 ataaaaacaa aagaccactc ttgtcgctca gctagtgttt tatagcctgt ttctttcaca
     4621 aattgagcaa attcttgatt cgtaactgga gtatccgcaa tggaaaaatc ctttacacta
     4681 acagcgactt gaggaccttc aaaatcatct aaaaagccct cttcagaatt tgttcccatc
     4741 ataaatgtcc cacccttgat ttttttcatt ttcatgattg actatccccc gttttcgtta
     4801 gagttggcgt tattttagaa agttcaacag gaactaactc ttcatccaca acaaaacctt
     4861 cttctctata tctaagaaag tctactaatt ttttcttcat ctgacgaaca ataggttgat
     4921 atttttcact tggatacaaa tcattcattt catgcggatc ttttttcata tcaaataact
     4981 gataatgatt taaaacagga aaccagataa atttccactg gtctgtcaaa atatattgac
     5041 tagaatcttt tcctaaagca tgttcaccat gaaattctgt tcgccacccc tcgtactgtc
     5101 caaataataa attcttaaca ctccgtccat ctagttcatc tgtagtagtt cctcctgcca
     5161 aatccacgag tgaaggaaaa atatcttgaa ttttcactaa ttgcttgatt gttcccctat
     5221 taccagctat caatccagcc ggatcgtaaa taaacgaagg aatgtggata cttccctgat
     5281 aaggatatcc ctttctaaac agataatgtt caccaagctg atctccatga tccgaaacaa
     5341 accagataat cgtatcttta tcatgacgaa attcttttag ggcagtcaaa aagcgactaa
     5401 tttgatggtc tatatgagta atcaatccaa aataagcagc aaccattctt ctttgatcat
     5461 cttcctttaa tttccctctt aaagcataaa tagacggaat ttgcttttct aatacttccc
     5521 aattcccgat atgcaaatcg agaaattgag gcaagcgttc catatacata tcaaaatagt
     5581 attttggggg atttaatgga gcatgtggtt tttcaaaaga cattttgaga aaaaaaggaa
     5641 ccgttggatc tcttctttgt aaaaatgaaa tagactcact cacaacccag tttgttggat
     5701 gtaacttctc atctttatcc caaggtctag cttcccagga attacaatcc attccgtcat
     5761 caatcaaatc aacatcataa ccaactttac cctttaaaaa ggctagatag tcactagcat
     5821 aatcaaattg acttccataa gtcttatcat actttctatc aacatgtaga tacccatcgt
     5881 gaagtaatac atgatcaaac ccaagtctct gtctggaagg aaaaacatgc atcttaccaa
     5941 tacactctgt ttgataaccc atatctttaa agacttttgg aagagtatta gtaaaattcc
     6001 aaggcacctc atcttgataa ccaacacgtc cacttttatc ttggtctaag ccagtcaata
     6061 aagctgccct agcaggaacg caagaaggaa ctggagaata ggcattttca aaattataac
     6121 ctacactagc catcatatct aatgttggag tgctaactag cttatcctta ctattcaagg
     6181 ataaggcgtc tgctctcatt tgatctacaa caatcaaaat aatatttggt tgttttgtct
     6241 gaaccataaa atctcctttc taatatggca aaagaggcac aagaagatat ctacctttac
     6301 tgcacccctt tctatatcaa tctctctata taaagcaata acattcttgt tatgttttat
     6361 agaaaaatga actaaaatat gactaaatcg attaggaaat tcaaatcatt ttctagtact
     6421 attttagaag ttacagtgta ctattccaac ttcaataaac tataaacctt tgtctaataa
     6481 caattttagt ggagataaga aatcctacac ctaactcaat cttacacgta atctatttct
     6541 attttatcac aaaaaacgca agtaagacca ttaactcaat tcagttttat ctgccatttt
     6601 cacaaatggg aaataagtca agacactaat aatcaaacaa acaactgata agatgatggc
     6661 acgccaatca aatgctgtag agaagcaacc atataaaatt ggaggcatta cccaagtaac
     6721 attttgtgta acaggtgaaa caagacccca gcttgttgcc cagtaagcta ccgttgccat
     6781 gaaaaccggg ctaagtacaa atggtataaa tagcaaagga ttcaagacaa ctggtaaacc
     6841 ataatttacc ggctcaccaa tattaaacag aactggtgct agaccaagtt tagcaacttt
     6901 tcgataatga ctgtttcttg aaaaaattaa aatagcaagt accaatccta atcctccaaa
     6961 ccagacaaac gccccaaaag acccacttgt ccatatataa ggaatcggtt cacctttttg
     7021 gaaagcatcc agattcgcta acatagcaac tccaaacagc ccttccatga tgggagccaa
     7081 tacatttcct ccatggagac caaaaaacca gaataactta tttaaaaaga tcatcagaat
     7141 aactgcaaag aaactttgag acaaacctag taatggcgtt tgtaacacct tgtaaaccca
     7201 atcaatcaat aagtcattgc taagtaaatg gaaaacataa gtcaagatgg ctactatata
     7261 catcgccata aatcctggaa tgatagaagt gaacggctta gcaatcgcag ggggaactga
     7321 atctggtaac ttgattaccc agttcttttt cattacttta cagaaaataa tagaggctaa
     7381 aaatccaatc atcatggctg taaagtagcc tctggcatta atatggtttc ctggaatcac
     7441 attcccaata gttaccatca gatttttacc atcaaatgct agattatcaa ttccttgtaa
     7501 gatttgatct aatttcacat ctcctacatt tgccaaaggg aaactctttg taactgtact
     7561 tccaatcgaa atgacaaacg aagcaagcga taccaaacca gcagaaactg tatcaacctt
     7621 ataaatctta gcgatattca ctcccaagca atagatgaac aataaggaaa caattggtat
     7681 acttcccttg aataccaaat tattgatgtc aacaagccac tgaaaggttt tcgtaatact
     7741 ttctaggtga aattgttgtg gtaaatccac tagaaaagca tttaataaca aagcaatgga
     7801 acctgtcata ataacaggca tagtccccac aaatgaatca cgtaaagcaa ctaaaaactt
     7861 ctgattccca actttcattg caattggaga aagtttttct tgcattttat ccaacattgt
     7921 attcatagca aattcctaat ttttttcaaa tcgttcgtat acttctacca cttctttcgc
     7981 caagtcaaca aaggtcagac tcgtcattaa atgatcttgc gcgtgtacca tcaagaggct
     8041 aattgatgtt tcttccccct gtgcctcttt tgtcaacatt tctgtttgag ccttatgcgc
     8101 ctgtaataag gcagcattcg catcagctaa tcttcgattg gctttagaga aatccccttt
     8161 ttttgctgcc tgaatagcct ccatagcatt acttttggca tccccaccat acataattag
     8221 ccccattaca gattctaaat tactttcatc cataacattc tcctcaatct tagtctaata
     8281 aactttcagc taaatctaaa actttagaac cattcatcat tccgtaatct gtcatcggaa
     8341 taaccgccac aggaatctgt ctatctttta gtttttcttg aaagtcccct agtaaatagc
     8401 gaacttgggg gcctaaaagc aacacattta cttcttttgt tgctacaatt tcttctgctt
     8461 caggagctgg aactgcaaaa atttctgcat ccaacccctt atcttctgct gccttttgca
     8521 tctttgtcac tagcagactt gtactcatac ctgctgcaca tgctaacata attgttactt
     8581 tagccatagc ttactccttt atcacctatt tactgaccta gccaagcgag atactgtatg
     8641 tctcactcct ctaattgcct tgctcaatgc ataaatatta tcgactgttg ccagtccccc
     8701 atatcctgcg tcacctatat gttgaatatc aactccacaa attttatttc taagtgcaat
     8761 ttccttaata gtatctgtat cagatgtttc ttggctagta ccaatagcac tcaatacaag
     8821 accaccctta ctatgaacga gatcaacgac ttcacgcaac tcttggtcat gaaaggctgg
     8881 aacggttcca actgctggaa caagtatcac atccgcacct gcttccaaca attgctctgc
     8941 gacagaaagc tctgccacag gctcattcac tcctgccccg tgcatcttac ctgcaataat
     9001 caaaccagaa aagttttcct tagcagtttg aacagcctta atgatttctc gattgctaac
     9061 tcctgttcca ggatttccag tcaaacagac aaagtcaaaa cctaactcct ctattcgatt
     9121 caatgtttca acactagcaa cacgacctgc aacaatttcc tgtgtttcct ctagcatctt
     9181 tgcagatggg tcaatcggtt ccaaatttac accaattgga caagcaacaa gctggtgtaa
     9241 gcgatgaata acatcatcac ctgaactatc caaagcataa attttaggct caaaaacatc
     9301 aacacaattc aataaaatca tatcagctcc aaaagctcta gcaatttcag aattagttat
     9361 atccccaaca aatgtctcac gagtaactac attttcagat agaacaactc taccttcact
     9421 tgctaaaata ctttgtttta attcttcagc attcatctgt aatatctcag atggatttgc
     9481 actaattaaa cgtttcattc tcaatcctcc aataacttaa accgtttttc aatagctatt
     9541 acaaatggta aataaagtaa gacatccaaa acaatcaata caatttgtaa taatgaacca
     9601 gaaatacttc ctgtagcaag taaaccactt ataacaggtg gcatagtcca acctggatct
     9661 gtataagtta gcggaaccaa acctgatgcc attgctcccc acgctactag taagttaaac
     9721 attggagcta agataaatgg aactagtaac aaaatgttca aaacaatagg aacgccaaac
     9781 attgctggtt catttatatt aaataatcca ggtataacag ttataggtgc caaagttttt
     9841 agttgtttac tagctttctt cttatgtgct aaatatccta acgcaagaac taaacctatg
     9901 gttgcaccac cgccacctat gaatacaaaa ttatccataa atacgctagt aaaaatattt
     9961 ggaagagttt ctccagtttg ataagctact ttatttgcat ctagattagc taaccataac
    10021 ggtttcataa ctgcattaac aacatttcct ccgtgtaatc ctacaaacca aaaactacta
    10081 ttaaggaaaa ttaagataag aagaccaata acattatttc ctaaaagtcc aagtggacct
    10141 cccaaagcta cttgagcaat ttcatgaaga ttaggtaaac ttaatttatc taaagttgca
    10201 taaacaatca accaaccaac aatagtcact gcaccaggaa taatggcact aaagctttta
    10261 gatactgctg gtggaacacc atccggcatt tttatctgta tattatgatt tataaaccat
    10321 tggtagatat agccattaat caatcctaaa atcatagcaa caaataaacc ttttgatgcc
    10381 atgaaagcgg taggcattcc tgctccagcc tctcctgtaa tgaaaggcgt aactgtaata
    10441 aaagatgata atgacacaat accagcagga ataccatcaa ctttgtattg tctagccata
    10501 aagtaagcga taccaaaact agctacaaga ccaagtaatc caaaactact atcaacaccc
    10561 ttccataaat agtcagcaac accaatatca cccagatatt tttcccatcc tggaatagga
    10621 aaacttgcaa taaccattag taatgaacct atcaacaata aaggtatagt tagagtaata
    10681 ccgtctcgaa tagaaaccaa cgctttatta ttacccaatc tagccgctac tggaagtaac
    10741 ttttcttgta ataatttttc cataaccttt tcctcttaaa tatattttat tatcaaaagt
    10801 tatcatttat ctttattttt tgacgtcaag ttgttatttg attatctttt gttatttata
    10861 ttttacaact ataagaaagc cttgtcaagg atttgtgtaa acatttgatt attttttatt
    10921 atcaatatat aataaaaaga gaccagtaag taatgaaaag cttaaaaaga ttaacatcta
    10981 catttatcca tagcaaaaaa tcataggatt ttaagaatct gacaactagg aagaaaacta
    11041 atatgacctt atctaagaaa caattacaac taagagctaa aatactggaa acagtttaca
    11101 cattaggtcc catttcacga attgaaatcg ctactaaaac aggaataaca cctgcaacaa
    11161 ctagcagtat cacaaatgat ctgattaaag aaaatattct cttagaactt ggtgaagacg
    11221 aacacgatac aagcgtaggg agaaaaaaaa tcctgctaga cattcaagct aaacgatttt
    11281 actacattgg ttgtgaacta tctgaaaaac atttcacttt tgctttagga gataatctag
    11341 gaaatatctt aaaggaagag aaagaaattg ttactaaaca gttaatccaa gaaaagggaa
    11401 atcaactcat taaccagaca ctaaagcaat tcttaaataa ctgctctgat tatgaaatag
    11461 aagcaattgg aatcgcgcta cctggtcgct atttagatga ttataaaata acaacaaaca
    11521 atcctttatg gcaacatatc gacttagaaa tgattcaatc ccactttgat aagcctttgt
    11581 ttttctctaa caatgttaac tgtatggcta taggtaaacg cctatttagt cgccaacaga
    11641 atgactctaa ttttgcttat tttcattttg ctagaggtat gcactgttct tatatctacg
    11701 acggaaacat ttatgggaaa ggaaatctaa tgattggaga aatagggcat acagttgttt
    11761 catctgaggg agaagaatgt agctgcgggc gcaagggatg cttacaaact tttgctggag
    11821 aatcttggtt aatcaaaaaa tcaaaaattc tatatcatca atctccctat tctctacttc
    11881 ctagtttagt caaaaatgcc gatgatattg atatccaagt tattttaacc gcttatcaat
    11941 taggtgacac tggaataatc acacttatcc atcaagcctt actctatctt agtcaaacca
    12001 ttttaaatat cagtatgatg attgactctc aaaaaatcta cctacatagt cctttactaa
    12061 caaatcaaca catcatccaa aaactatata gtgaaatgaa ctacaaaccc aaactgctct
    12121 ataatcgcct acctgaagtc attattgaac cttacaatga ttttacagcc gcacactcag
    12181 ctattgcact ttgtttgtat catacaattc tacattctta attttctatt acatttaata
    12241 aataggctaa aaagagggag tatatcataa aaagcgaata aacctgtttt ctgaaaatca
    12301 gaattctgtt ttgttcgctt tctttatatt tggaattata ctcctatctc ccatttttca
    12361 taatgtatag taaaatgaaa taataacagg acgaatcgat cgggacagtc aaatcgattt
    12421 ctaacaatgt tttagaagta gaggtgtact attctagttt caatccacta tattttcgta
    12481 caggtgcttc aaccatttga acgatttcaa atccttcttt ttggtaaaga ttctgagctc
    12541 tttgatttgc ctcgaagaca tttagagaaa tattatctat atcttcattt tcaaaggcca
    12601 aactaacaaa tttccttaaa gtctggctac ctaagccttg cccctgtttc tgggggttga
    12661 taaaaaatct cccgatatga agattgctgt cttttagcct gattttctgg ataaggccca
    12721 caaactcttg ttcatcaaag attgaaaata cttcttccaa ggcttgaagg gtcagtagaa
    12781 aaggaatcct tggtcccatc cattgttctt gaaaggattt gcctagggag ttggaccatt
    12841 ggcatacaaa ttgagcgttt tctatactca ccttttcttc aaaacgaatt gtcatctttt
    12901 cctcaccatc ttatctatgt ttctccatta tactatttct cccatttttt acgaatagat
    12961 aagtatgatt gatttttatt tttttctcgt cgggagcatt ctcgcttcct ttcttggttt
    13021 ggtcattgac cgttttccag agcaatccat tatcagttca gccagtcact gcgattcctg
    13081 tcagactcgc ttgcgtccct tagatttgat tccgattctc tcacaggtct tcaatcgctt
    13141 tcgctgtcgc tactgcaaag ttcgctatcc tgtctggtat gccctctttg aactaggctt
    13201 aggactcctc tttctgcttt actcttggga attgctttcc ttggggcaag tcgtcctaat
    13261 caccgctggt ttgaccttgg gtatctacga ctttcaccat caggaatatc ccttactggt
    13321 ctggatgact ttccacctaa tcctaatagc ttcctctggc tggaatctgg tcatggtctc
    13381 cttccttgct cttggaattt tggctcattt tatcgatatc cgcatgggcg caggagattt
    13441 cctcttttta gcttcttgtg ctctcgtctt tagcgtaacg gagttactga tcttgattca
    13501 gtttgcttct gcgacgggta tcctggcctt tctcctgaaa aagaaaaagg aaagacttcc
    13561 tttcgtgcct ttcctcttac ttgctacttg tttgattatt tttggtaagc tactgcttgt
    13621 ctgataaaat cctgaatcgg ctctccttgg tggagagctt ttactatttt tgaaccgacg
    13681 ataacaccat ctgacaccgc attgaagcgt tccagatcgg cttgactaga tacaccaaaa
    13741 cctgtcaaga ctgggatgtc ggccacttga tgaagttgcg ccaagtgctt gtccaaatct
    13801 gcacggtaat tgcctgattt ccctgtcact ccattgatgg caacggcata gacgaatccc
    13861 tccgcccctt caatcaactc tttctggcgc tcaattcctg tggtcaagct tactaaagga
    13921 atcaaggtga tatctgtatc tgccaaaaat ggttctacaa agttggcatg ttcatgaggc
    13981 aagtctggga taatcaagcc cttcacagct gtatcagcca gatctttgac aaagttctcc
    14041 acaccgtact gaaagagggg gttgaagtag gtcatgatga ccagtggaat ctctgtttca
    14101 atggttttca aggtttcaac taaagcctgg gtagaggtcc cgtgggctaa actgcgcaag
    14161 ccagcttctt cgataacagg tccatctgca acagggtctg aaaagggaat acccacttca
    14221 attgcagaga cacccaaatc ttctaaaaag tgaattgttt cagcaagacc gtccaaacct
    14281 ttctcgtggt caccagccat gatataggga acaaaaattc cttttccagc tgctttaata
    14341 gcattcaatt tttctgttag tgtcttaggc atgagcttct cccttctttg ctgcatctgc
    14401 ttccaagcgg tctttgactt gaaccacatc cttgtcccca cgacctgata ggcagacaat
    14461 catagacttt tctggtccaa gttctttggc caatttcacc gcaaaggcga tagcatggct
    14521 agattccaag gctgggataa tcccttccac acgagacaag agttggaatc cttccaaggc
    14581 ttcttcgtct gtcacaggga catagctggc acgtttaata tcgtggtagt gagaatgctc
    14641 tggaccgata ccaggatagt ccaaacctgc tgagatagag aaggcttcaa gaatttgacc
    14701 atgggcatct tggagcacat ccatgaggga accgtgaagg acacctggac gacccttggt
    14761 caaggtagct gcgtggtgct ccgtatccac accaagtcca gccgcttcag ctccatacat
    14821 ggctactgac tcatcttcta caaagggatg gaagagaccg atggcattcg acccaccacc
    14881 aacacaggct actagggcat ctggcagatt ttgtcctgtc aagtcacggt actgttgttt
    14941 agcctctcga ccaatgacac tttggaagtc acgaacaatt tctggaaatg gatgaggccc
    15001 caaggcagaa ccaaggatat agtgggtatc atctatatta gccacccatg aacgaagagc
    15061 tgcattgacc gcatccttga gcacgcgcga accatctgtc acggcctcga ccttagctcc
    15121 caaaagctcc atacggaaga cattgagggc ttggcgtttg acatcttcct cacccatgta
    15181 gatggtacat tccatgttaa agagggccgc agcagttgca gttgccacac cgtgctgacc
    15241 agcaccagtt tctgcgataa ttttcttttt acccatgcgt ttggcaagcc aaacttgtcc
    15301 taaggcattg ttaatcttgt gggctcctgt atggttaagg tcttcccgtt tgagataaat
    15361 cttggctccg ccgatatgct gggtcaagtt ttttgcgtag taaaggggag tttcacgtcc
    15421 cacatactgg cgcaaaagtt ggtttaattc ctcttggaaa cttgggtctg cctgactttc
    15481 acggtaggcc ttctccaact ccaaaactgc tgtcatcaat gtttctggga caaaacgtcc
    15541 gccgaatttt ccgtaaaatc catctttatt tggttcttga tatgccattc tttaccctct
    15601 ctataaatct tctaatcttt tcatgatctt tttgtccatc tgtctccact ccgcttgata
    15661 catctactgc atagggagta aagtgttgaa ttgcttttac tacattgtct tcattaagcc
    15721 cacctgcgat aaagaaagac tgaggtaatc cagtcgtatc cagttgaccc caatcaaagg
    15781 gctggccact cccagccaca ggggcatcaa agagtagata gtctgcctga gaattgggca
    15841 catgaccctc tccatctacc tgcacagcct gaatgctagc acaaggcaaa ttctcaaata
    15901 aatcatctgc cacctgaccg tgaacttgaa ccaagtccaa gccaactttg tcaatcgctt
    15961 ccagcagttc tacccgactt ggtgaaacaa atactccaac ctttttcaca tctgcaggaa
    16021 taagctttgc caactcagct gcctcttcta aagtcacctg tcttttacta ggtgcaaaga
    16081 caaaaccgat atagtcggct cctgctgaaa cggctgtttc caccgcttct ttggtcgata
    16141 gtccacaaat tttaaccttt gtcaatctgc aactccttga ttctctgggc cacattttct
    16201 gcctgcataa gagctgtccc taccaaaatt ccgttaaagt atggggctag tcgttccgca
    16261 tcctgccctg tgaaaatggc agattcagaa atgtaatagc gaccttcctc aaagtaaggg
    16321 gctaaatcta cactggtctg caagtcgacc tcaaaggtag tcaagttgcg gttgttgacc
    16381 ccgataatct cagcaccaag tctgtgggct acctctagtt cagctagatt gtgagtctcc
    16441 actaagactt ccagaccaag ctctgtcgcg tagtcataca gttccttgag gcgttcttcg
    16501 gacaaggctg ccacaatgag caagataact gtcgcacctg cattgcgagc gcggatgatt
    16561 tgcttttcat ctatgataaa gtctttgttg agcgtcggaa tctctacctg actggaaatt
    16621 tcccgtagat aatccaaatg ccctttaaag aaaacctcat ctgtcaacac cgaaatcatc
    16681 actgctccgt tttcttcata agtctgggcc tgttgcacaa tatccacatc gagattgata
    16741 tctcccaaac tagggctagc tttcttgacc tcagcgatta cctgcaagcg gtcctgatga
    16801 ttcttcaaaa attctgccaa gcgataggtc tggcgcagag gctggatttg ctccagcttc
    16861 atctgctcca cctcacgcgc cttctgctct aagattcgtg ctaaaaattc ctgactcatt
    16921 tttggtactc ctgtaacagt ctgagttttt caagggcctt gcctctagca atcacttgac
    16981 gagccaaggc aactccttcc ttgatgctat caatcttacc attagcatag aaaccaagac
    17041 cagcattcaa gactgtcgtt tccaagaatg gacttgcttc gtttttcaga acgctaagca
    17101 aaatttctgc attttcctga gcattcccac cacgaatatc ttccatagca tagccttcca
    17161 ttcccaaatc ctctggagta aagcttgaca agctgatttc gccattttca agaagtgcaa
    17221 tcttggttgt tccgttcaag ccagcttcat ccaacccttc tggtccagca accacgatgg
    17281 cacgtttgcg acccatattt ttcaaaacct gagctgtact ttctaggagt tctggacgac
    17341 taattccaag aagctgtgtt tctaaagcca ttggatgaat cagtggacca gtcaagttca
    17401 taatcgttgg aattcccaat tccaaacgag ctggcatgat gtatttcata gctgggtgca
    17461 tatttttagc gaagagaaag acgattccag ttttatcaaa gaccttacct agttcagctg
    17521 gtttgaggtc aagattgatt cccaaggctt cgaggacatc tgcggaacca gatttagaag
    17581 aaatcgagcg gttaccatgc ttggccatgt gaataccgcc accagccaag acaaaggctg
    17641 cagttgtgga aatattaaaa ctgaaagact tgtccccacc tgtaccacag ttgtccatgg
    17701 catcatgaat ctcagttgga atatgctggg catgtcctct catgacttgg gcaatggctg
    17761 tgcgttcttc aggtgtttcc cccttcatct taagagctaa gaggagagaa gcaatctgcg
    17821 cttcagttac acgcccagtt acgatacgct caatgacatc cgtcatttcc acacctgata
    17881 aattttcaaa ttttgctagt ttttcaataa tctctttcat cctagtttcc tcactttaca
    17941 accttctcga taaaattccg aatagaagac aagccgtctg gcgttccaat gctctctgga
    18001 tggtactgga agccataaat cggtaggttt ttatgttgaa tccccatgat ggcttggtca
    18061 tcagtcgaac gagctgtcac ttcaaagtct tctggcattt cctcaatcaa aatactgtga
    18121 taacgcatga ccgcacgtcc ttcctcaata ccttgataca aaacagatgg cgcttcaaag
    18181 ttgatattgc tctgtttccc atgcatgact tttggagcca aacctagctt accaccaaag
    18241 acttctgcaa tggcttggtg gcccaaacaa atcccaagaa tcggcttctt gcctgcaaaa
    18301 tcacgaatca tgtcttccat ctttccagca tcaactggcc aaccaggacc aggagaaaag
    18361 accagaccat ctgctttttc agcttcttca tacagcttgg aatcatcatt tctcagaacc
    18421 tgaacttctg caaaattccc aatgtattgg gccaagttat aggtaaaaga atcatagttg
    18481 tcaatcaata aaatcatggt cttagttctc caattctagt catagatttt gctttgttaa
    18541 tggtttcttg gtattcgttt tgggcgatag agtcgtagac aatccctgcc ccagcctgca
    18601 cataggctct ttgatttttg agaatcatgg ttcggatggc aatggccaaa tccatatcac
    18661 ccgtcgcaga caagtagccg attgctcctg catatacgcc ccgtttttcc gtttccagtt
    18721 catagatgcg tctcatcgcc cgaatctttg gtgctccaga aacggttcca gcaggaagtg
    18781 tagctttcaa ggcatccatg gccgtgagtt ctggaagcaa acgccccttg accacgctgg
    18841 tcaaatgcat gacgtagcgg aagagctcca ccttcatata cttagtaact tggacactgg
    18901 ccgtttcaga gatgcggcca atatcgttac gccccaagtc taccaacatt cgatgttctg
    18961 ctgtttcctt ctcatcagag aggaggtcag tcgccaaggt cttgtcttct tcatccgtag
    19021 cccctcttgg tcgcgtccct gcaatcggat tggttgtcac gatgccattt ttgacagaaa
    19081 ctaaactttc tggactagct ccgatgattt gataatcccc aaaatcatag aaataaaggt
    19141 aattagaagg attagtcacg cggagatttc tgtagaagtc aaatggattt ccagtaactt
    19201 ctgctgaaaa acgctggctg agtacacatt ggaacatatc tccgttacga atcaagtcac
    19261 gagctgtttc taccattccc tcaaacttat gtggagcgat atgcggtttg aagtctaacg
    19321 gagatagatc caaatcttca aattcatttg gagcaggaat gcgtaattcc tcaagcactt
    19381 ggttcaagga tttttccaag gcctcttgac tgcgctcact ataaagtgca tcctctatga
    19441 catgtatctt ctccttcttg tggtcaaaga ccatatagct ctcatagaca aagaaatgca
    19501 tgtctggcgt cccaattgta tcctcaggga tttgaccaat ttcttcataa agcgaaatca
    19561 tatcgtaacc cacaaaacca atggctccac caccaaaagg tagctctgag tggtgctgac
    19621 tcttatgaat cacttcataa aggaaatcca agggatcccg atcaatcact tgaccatttt
    19681 gatagagaac cccattttca aacttaatct caaaaactgg attataggct aggatagaaa
    19741 aacgagctgt ttccttgtct ctcggaatac tctctaaaat aaccttatgt tgccccttta
    19801 agcgcatata agccaagatt ggtgataaga catctccatg aatgattcgt tccattgtaa
    19861 tttccctttc agttctactt ctagtccgtg gtgactgtat gaaaaatccc cacgcaaaat
    19921 aacttgcgtg aggacgaaat tcgcggtgcc acctcaatta taggatttct cctatctctc
    19981 attcctgtct cagatatctc ctgtaacagg ctgtgcgata aagggcactc ccttgagaat
    20041 gatgttttct tctctcgttt cagatgaacc caactttaca gctttctctg cttgttttca
    20101 gcaaccacaa gctctctgtg agagaaagaa ctgtaatttt tccatctatt attttttagc
    20161 ttctagtaat ctgcaatcgc agctaggtcc ttgcctccac gaccagagac attgatgaag
    20221 agatgttcat ctcggtacac ctttatactc ttcgaaaatc tcttcaaacc gcgtcaacgt
    20281 cgccttgccg taggtatggt t
//
LOCUS       DAVKWZ010000035        20215 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000035 DAVKWZ010000000
VERSION     DAVKWZ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20215
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.035"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(63..1004)
                     /locus_tag="VW854_001347"
     CDS             complement(63..1004)
                     /locus_tag="VW854_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV0262574.1"
                     /translation="MKQAKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(982..2745)
                     /gene="aspS"
                     /locus_tag="VW854_001348"
     CDS             complement(982..2745)
                     /gene="aspS"
                     /locus_tag="VW854_001348"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEV0262575.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEALAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSAEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETSKTN"
     gene            complement(2981..3061)
                     /locus_tag="VW854_001349"
                     /pseudo
     CDS             complement(2981..3061)
                     /locus_tag="VW854_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(3097..3327)
                     /locus_tag="VW854_001350"
     CDS             complement(3097..3327)
                     /locus_tag="VW854_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262576.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            complement(3345..3986)
                     /locus_tag="VW854_001351"
     CDS             complement(3345..3986)
                     /locus_tag="VW854_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0262577.1"
                     /translation="MKLLKNLGWILLALLSFLFIYGFIQGLATASLALGASPYAVTLL
                     YVALAGVYVYGIYKWYQKAPVHIEKSGFNRFIWLPVLVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            complement(3998..4732)
                     /locus_tag="VW854_001352"
     CDS             complement(3998..4732)
                     /locus_tag="VW854_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEV0262578.1"
                     /translation="MKKKRRLLFLMSIVLGGFLGMFVGMFKARVESHEIILDVKALMP
                     WISAICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAF
                     NITGVAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLE
                     FFATPKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQL
                     LAFLLVGAIHVYINVMQLPMVKRYFK"
     gene            complement(4735..4932)
                     /locus_tag="VW854_001353"
     CDS             complement(4735..4932)
                     /locus_tag="VW854_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262579.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVTEWIEKKSKNLASEKGE"
     gene            complement(4932..5138)
                     /locus_tag="VW854_001354"
     CDS             complement(4932..5138)
                     /locus_tag="VW854_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0262580.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            complement(5148..5462)
                     /locus_tag="VW854_001355"
     CDS             complement(5148..5462)
                     /locus_tag="VW854_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262581.1"
                     /translation="MWVLGFILFMIFFYSNNSKKIKKLENKIKKLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEFVDVDEEWVKLRSVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            complement(5732..5812)
                     /locus_tag="VW854_001356"
                     /pseudo
     CDS             complement(5732..5812)
                     /locus_tag="VW854_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(5803..7092)
                     /locus_tag="VW854_001357"
     CDS             complement(5803..7092)
                     /locus_tag="VW854_001357"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEV0262582.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIVMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQAHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVALPIENALDVYIA
                     VLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFATKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(7170..8417)
                     /locus_tag="VW854_001358"
     CDS             complement(7170..8417)
                     /locus_tag="VW854_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEV0262583.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTFFTVFAGIRADYTRDKVKWIVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLDLSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAIIISSLTGNAYFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNMQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
     gene            complement(8608..9459)
                     /gene="rgg"
                     /locus_tag="VW854_001359"
     CDS             complement(8608..9459)
                     /gene="rgg"
                     /locus_tag="VW854_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HEV0262584.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIMIKSYMKSIDERVDLTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKFDFLMEMSGNRNLFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKIEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            9583..9924
                     /locus_tag="VW854_001360"
     CDS             9583..9924
                     /locus_tag="VW854_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV0262585.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            9982..10062
                     /locus_tag="VW854_001361"
                     /pseudo
     CDS             9982..10062
                     /locus_tag="VW854_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(10313..12016)
                     /gene="ilvD"
                     /locus_tag="VW854_001362"
     CDS             complement(10313..12016)
                     /gene="ilvD"
                     /locus_tag="VW854_001362"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEV0262586.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(12249..13181)
                     /locus_tag="VW854_001363"
     CDS             complement(12249..13181)
                     /locus_tag="VW854_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEV0262587.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSYSDIVVLEADLSSSMATHNLEK
                     NFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSTIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(13178..14035)
                     /locus_tag="VW854_001364"
     CDS             complement(13178..14035)
                     /locus_tag="VW854_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV0262588.1"
                     /translation="MILSENREDELRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEEKQMLTSVVER
                     LSQELEETE"
     gene            complement(14039..15385)
                     /locus_tag="VW854_001365"
     CDS             complement(14039..15385)
                     /locus_tag="VW854_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV0262589.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACIIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFIGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSAKEE"
     gene            complement(15398..15682)
                     /locus_tag="VW854_001366"
     CDS             complement(15398..15682)
                     /locus_tag="VW854_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV0262590.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(15686..17716)
                     /locus_tag="VW854_001367"
     CDS             complement(15686..17716)
                     /locus_tag="VW854_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEV0262591.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLTDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLINQVVKDYYEKLQLVPIVNQLKVCLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            17908..18915
                     /locus_tag="VW854_001368"
     CDS             17908..18915
                     /locus_tag="VW854_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV0262592.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNGNDVNLEIGKKHISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            18918..19118
                     /locus_tag="VW854_001369"
     CDS             18918..19118
                     /locus_tag="VW854_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV0262593.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            19270..19452
                     /gene="rpmF"
                     /locus_tag="VW854_001370"
     CDS             19270..19452
                     /gene="rpmF"
                     /locus_tag="VW854_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEV0262594.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            19468..19617
                     /gene="rpmG"
                     /locus_tag="VW854_001371"
     CDS             19468..19617
                     /gene="rpmG"
                     /locus_tag="VW854_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV0262595.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
BASE COUNT         6450 a         4316 c         3547 g         5902 t
ORIGIN      
        1 aacatatata cggcaaggtg aagctgacgt ggtttgaaga gattttcgaa aagtattaat
       61 cactaattat ctatctcaac aaatcttcct agaatatgaa cattttccga gacagagaca
      121 aaggagcttg gatccacttg tgtcataatc tgtttaaatt cattaaactc tgcacgtgta
      181 atgacagtga ttaaaactgc ctttctctcg tgattatagg ttccttctgc atcgtggatc
      241 atggttgctc cgcggtgcaa ttttttatgg attttttcaa ttaccttctc tggatgattt
      301 gtcacaatca tggcctgcat acgcttttgc ttagtaaaga ctgcgtctgt cacacggcta
      361 gagacaaaga tggtaatcat agaataaaga gcgtatttcc aaccaaaggt taaacctgct
      421 atcagcatga tagttccatt taccaagaaa gaaatactac cgacattctt acccgttttc
      481 ttgcgaatag tcaggctgac gatatccgtc ccaccactgg agatattgtt tcgaagagca
      541 aaaccaatcc ccaaacccat aacaacaccc ccaaaaaggg aattgataat gggatcctcc
      601 gtcaaggttg ccacagggac aaactggata aagaaggaac tcatagatac cgtgataaag
      661 gtaaagacgg tgaacttatg gccaatctga taccaagcta agaccatcaa agggaagtta
      721 atggcgtaga agcttagcga aatcggaata tgaaaaccaa accagtgatt actcaaggca
      781 gagataatct gtgccagacc tgttgcacca ctcgaataca catgccctgg ttggaaaaag
      841 aaattaactg ctactgctga taaaaaacca tagaccagag aggccgaaat cttctcatca
      901 tacttttctc gagagatact ttgtaagaca cgtaaaattt ttatctgata agcaaagcgg
      961 cgcagataat agcgccaccg cttaattcgt tttgcttgtt tcatcttctt ctacttgtaa
     1021 gctgagttcc tctagttgtt tgagagcgac tgttgatgga gcttgtgtca ttgggtcagt
     1081 tgccttgttg ttcttaggaa aggcaatgac ttcacggata ttttcttctc cagcaagcaa
     1141 catgacaaaa cggtcaagcc cgatagccaa accaccgtgt ggtgggaaac catagtccat
     1201 ggcttcaaga aggaaaccaa actggtcatt ggcttcttca gctgagaaac caagagcctt
     1261 gaacatgcgt tcttgaaggt ctttttggtt gatacgaagg ctaccaccac caagctcata
     1321 accgttcaag acgatatcgt aagcaatggc acgaacctta gccaaatcac cttctaattc
     1381 gtgagcagtc tcttcctgtg gaagtgtgaa tggatggtgg gcgctcatgt agcggccttc
     1441 ttcttcagac cattcaaaca tcggccagtc aaccacccaa aggaagttga acttatcatt
     1501 atcaatcaag ccaagctctt tagcaatacg tccacgaagg gcacccagtg ttgcattagc
     1561 cacttcaagc gtatccgcca caaagagaac caagtcctta tcttcaagag caagcgctgt
     1621 tgtcaattct tcttggatac cagtcaagaa cttggcaact ggtccgttta attctccatc
     1681 aaccaccttg acccaagcaa gacctttggc accatactgt ttggctactt ccgtcatctt
     1741 gtcgatgtct ttacgtgaat agttgtccgc agctcctttg accacaatcg cttttacagc
     1801 aagtgcttct gaaaagactt taaagtctac acctttgacc acttctgtca agtcctgaag
     1861 caacatgtca aaacgagtat ctggcttgtc agaaccgtaa agagccatag catcatcgta
     1921 tttcatacga gggaacggta gcgttacttc gatgcctttt gtttccttca tcacgcgcgc
     1981 gatcaagcct tctgtaatat cttggatttc ttgctcagta aggaaggacg tttccaagtc
     2041 gacctgagta aattcaggct ggcggtctcc acgcaagtcc tcgtcacgga aacatttaac
     2101 gatttggtag taacggtcaa aaccagcatt catcaagagc tgtttcgtga tttgtggact
     2161 ttgaggaaga gcgtaaaaat gccccttatt aacacgagac ggcactaaat aatcacgcgc
     2221 cccttcaggc gttgacttag aaaggaatgg tgtctccacg tcgataaact ccaactcatc
     2281 caagtagttg cggatagagt gggtcacctt ggcacgaagt ttaagatttt ccaacatttc
     2341 tggacgacga aggtcaaggt aacggtaacg caaacgtgta tcgtcatttg cctcaatgcc
     2401 atccttaatc tcaaatggtg ttgtcttagc tgtgttaagc acaataagag ctgtcacgtt
     2461 taactcaacc gcaccagttg gcaacttatc attggcttgc tcacgcgcag cgacctgacc
     2521 agtcacctca ataacaaatt cgctacgaag gctttcagct gttgccataa cctctgcaga
     2581 tactttttca gggttgataa ccaactgcat gattccttca cggtcacgaa gatcgataaa
     2641 gatcaaacca ccaaggtcac gacgacggcc aacccatcct ttcaaggtta tttcttgtcc
     2701 gatgtgttcc tcacgaacac gaccagcata catactacgt ttcattattt ctctcctctt
     2761 ttattctgtt actattttac cataaaagcg caggctcttc atgaaaatca tcagaaaagt
     2821 ttgccagtct ttaaaagtca ggtgaaagcc ctaaaaatta gcgctaatac tcttcgaaaa
     2881 tctcttcaaa ccacgtcagc gtcgccttac cgtaggtatg gttactgact tcgtcagttt
     2941 catctacaac ctcaaaacca tgttttgagc tgacttcgtc agttctatcc acaacctcaa
     3001 aacagtgttt tgagcaacct gcggctagct tcctagtttg ctctttgatt ttcattgagt
     3061 ataatacaaa aatccgatga acttcaccgg actcttttat tttgaatttt tgcctgcttt
     3121 acgcttttca gcgatttcgg ctgcctttcg aggcaagaca atttccgtta tgtaagccgt
     3181 cccaaaacgc agtacacctg caataggagc aaagacaact gctagatagt tatagaagaa
     3241 atcgcctttg aaggcataag ctagcgctcc aatgatgaaa aatagaacga ctgcctgaat
     3301 cactgctaat aaaattactc gtttcatgtg acctcctgac tctattatag catgagaatc
     3361 atcaaaaagc cgactaaatt attcaaagcg tgaagagaaa tactgtagac cagacctttt
     3421 ctgctaatgt aagccaaacc caaactaaaa ccaaggctaa aatagacaaa aaattgttgc
     3481 acatcacctg gaaaatgaat caaggcaaat agaagactag ataccagaag aaaaatcagg
     3541 gttcgtttac tattgtcctg cttaggaaag agatagcgtg ctaacatccc tctaaaaaca
     3601 atctcttccg tcaaaggagc aaaaataacc acagcaaaga atgagaaaag tggttgagac
     3661 aaggtcaagt ctgtcgctat ttgctgattt actgaaggat catctggcaa gaagaattga
     3721 acgaccagag ataagaacca aaccaagaca ggaagccaaa taaatcgatt aaagccgctc
     3781 ttctcaatat gaacaggagc cttctgatac catttgtaaa tgccgtacac atatactcca
     3841 gccaaggcca catagagtag agtaacagca tagggtgaag cgcctaaagc aagcgacgca
     3901 gtcgcgagcc cctgaataaa gccatagata aataaaaagg atagaagggc tagaagaatc
     3961 cagccaaggt ttttaagtaa tttcatagat aactccttta tttgaaataa cgttttacca
     4021 taggtaactg catcacattg atataaacat ggatggctcc tacaagcaag aaagctagta
     4081 actgaatctc tcctgtcaag aaagaaatga taataagaaa aatatataag gctggtaaga
     4141 catattggtg taattggaat aaaattcgaa aactctgttc caaattagcc tgacgctccc
     4201 cttcatcata agaatttata tagttcaaga catcctttgg tgtagcgaaa aattccaaat
     4261 caaactgacg aacaatcgca atggttttaa aaagagattt ttgagcgact aagaatacca
     4321 caaagagtaa gaaagaaagg aaaaatgttt gagggtttgt atgcaatata atcacctcac
     4381 ttaatgaaat aaaaatagcc aatggaatcg ctacacctgt aatattaaaa gcaatggttc
     4441 caaactcaag attccgatac atttgcacat aataggtttc attcagatcg tcatccattt
     4501 cctcttgata caaggaatga aattttctgc ttttctttaa gaaattgaaa gtcaaaaaca
     4561 tactaatgaa acctatcagt aaacaaatag ctgatatcca tggcatcaag gcttttacat
     4621 ctaaaataat ttcgtgggat tcgacacgtg ccttaaacat ccctacaaac atgcccaaga
     4681 acccccccaa gacaatagac atcaaaaata acaatctacg tttctttttc atattcattc
     4741 tcctttttca cttgctagat ttttggattt cttttcaatc cattcagtta ctgggatgag
     4801 agcaaagtag acccaaacaa attggtcgct ttgataggga ttaaaccagc ttaggtccat
     4861 cccaatcagt agaaatacgc tgactaataa agctatgacc actacataat aaatcacttt
     4921 atacttgttc atcactcgtc ctcctccaaa cgaaataccg attcgactgt ttcgttgaaa
     4981 atttgagata ttttcagggc aatgataatg gatggggtgt actcatcccg ttctagtagg
     5041 ctaatggtct gtctggaaac ccctgccagt ttggctaggt cggtttgatt gagaccatcg
     5101 cgagctcgaa gctcttttag acgatttttt agttgcacgt tacacaccta ctctccgtca
     5161 aattcaacgg tttggatatc ttcaatacgt tgcaacttga atttttcttt tcccgtatta
     5221 tcaacactac gtagtttgac ccattcctca tcaacatcca caaattccca gttatctggc
     5281 ccaatataca ctcccgttat aattggttcc tttccaatca tttcttgtaa taatctcgac
     5341 atttctgcgt ttcctttctc ttttcgctca agttttttga ttttattctc tagtttcttg
     5401 atttttttag aattattaga ataaaagaaa atcataaata gtataaatcc tagtacccac
     5461 attataactc ctttctgctt cctatttctt aacttgaatt cattgtaaca tatctttttc
     5521 tttttgacaa gtatagctgt caaaaagttt atgatttttg tcattttgca aaagaaaaag
     5581 gtcaggagta agttcctgac cactttatct atcattaata ctcttctaaa atctcttcaa
     5641 accacgtcag cttcaccttg ccgtaggtat ggttactgac ttcgtcagtt tcatctacaa
     5701 cctcaaaacc atgttttgag ctgacttcgt cagttctatc cataacttca aaacagtgtt
     5761 ttgagcaatc tgcggctagc ttcctagttt gctctttgat ttttattgag tataaaatcc
     5821 tagtttttca aagatttctg aaaagttttg gctgattgtc tcaagtgaca cttgcacttc
     5881 ttctcgggtt tggttgttct tgaccgtcac ttgtccgctt tcgacttcgc tctctcccag
     5941 ggtgatgagg gtcttagtcg caaagacatc ggctgacttg aactgagctt tgagcttacg
     6001 gttgaggtaa tcacgctctg ctttgaaacc ttgttggcga agggcttgga ctagttctag
     6061 ggctttgaca tttgctccat cacccaagac tgcgatatag acatctaggg cattttcgat
     6121 agggagggcc acgccttgtt tttcaaggat gagaagcaga cgctctacac caagcccaaa
     6181 accaaatcca gcagtttcag ggcctccaaa gtaagcaacc aaaccatcgt agcgaccacc
     6241 cgcacagact gtcaagtcat tgccctcaat ctctgtgata aactcgaaaa tggtgtggtt
     6301 gtagtagtcc agaccacgca ccatattggt atcgatgatg taatctactc caagattttc
     6361 caacatctga cgcacagcat caaaatgagc ttggctttct tcatcaagaa agtccaagat
     6421 agacggcgca ttctctactg ccaccttgtc ttctttttcc ttagagtcca agacacgaag
     6481 aggattttcc tccaagcgac gttggctatc cttagacaag gtctccttga gcggtgtcaa
     6541 atagtcaatc aaggcttggc ggtaggctgc acggctctca ggatttccaa gagtgttgag
     6601 gtgcaatttg acaccttgaa taccgatttc cttcaaaaaa tgggctgcca taacgattgt
     6661 ttccacatcg gtagctggat tgctagagcc aaaacactca acaccaatct ggtggaattg
     6721 gcgcaagcgc cctgcctgtg gacgctcata acggaacata ggtcccatat agtagaactt
     6781 gcttggcttt tgcacttctg gggcgaagag tttattttcc acataggaac ggacaacggg
     6841 cgcagttcct tctggacgga gggtaatatg acggtcaccc ttgtcataaa aatcgtacat
     6901 ttccttggtt acgatatccg ttgtatctcc gacagagcgg ctgataacct cgtaatgctc
     6961 aaaaataggc gtgcgcactt ctgcatagtt atagcgcttg aaaatctcac gggcaaagcc
     7021 ctcaacgtac tgccacttag cagactcagc aggtaaaata tcctgcgttc cttttggttt
     7081 ttgtaatttc atagggaatc ctctttaaac ttaatagtct tattttacca taaatagagg
     7141 gattaaaaca gtaagaaaaa aattaggatt tagatatcat ttttgagatt aagaattgtt
     7201 aaaaaaatag ctagcaagga aagaccaaca aatagcatcc aagtcaactg catgttccat
     7261 acggctacta gtgaaaaaca agctgttccc acaggtatgg ataaggtaaa caatagacct
     7321 aaaaaattac tagtacgagc tagaacctct ggagctagat ttttcatgag catggcacta
     7381 atctttggtt gaactttacc agacacatac agagtaaaga agagaaatag caaaccaagc
     7441 acgacttgat tgaataaatt agccaaacca actagactaa gtcctacggt cgcccacatc
     7501 atcaatctag gcaaggactg cttcccaaaa taagcattgc ccgtaagact actgatgatg
     7561 attgctacta aaacacagaa ttgattgata aatagtgcct ctgtataaga aaaattcaag
     7621 agagaatggc tcaaaaagaa gatattataa attccaccca aagcgccacc caaggaatta
     7681 ataagcaaga cagcaaagag cataaaacca aagtttttct gtccactttt aagaaaaacg
     7741 agacgtaaat ttcggtaaat tgttaggaac tggtctttga tagaaagctt ctcatttttt
     7801 aagttttcac catcagcaga tgacattgac aggctcaatt tgctttttcc taaaaagagg
     7861 atagtggctg atactaggaa aaagcaggca ttgattcccg caacgagaga aaaattgttg
     7921 accgatagat ctaagagcca gactccgaaa gcttgaccac caatagctga aatataggtg
     7981 atgaactgtg aaaaagaata agcctccatc agatcatctt cagctacttt ttccttaata
     8041 agaggcatac gcaggccacc tgcaaaatca ctgatgatat cactaatgac attgatcaaa
     8101 cacaggctag aaaaggcaaa gagactagct tgctgaacaa ctagggctgc tagaaaaaat
     8161 agaaccgcct gaaacaaacc gctatagact atccatttga ccttgtccct cgtgtaatct
     8221 gcccgaatcc ctgcaaaaac tgtaaagaag gtcggaagaa tcatgacaat attcgccata
     8281 gcaacagcaa aagatgcttg tgacaaggtc gatgcataga cgataaagac caggttgaaa
     8341 atcgaagcac caaaagcatt gaagaagcgt gataaagtca aaattctata agctggattt
     8401 ctaaacaata gtttcataga taatctccct ccttgttcta agttctacat caagaatagc
     8461 tttagtatag cacttatgaa tttgcgaaac gattcttttg aatatatgca acaaacaaaa
     8521 tgagcaaaaa aacgatagaa cttttgtaat gacctgagag agggcaacag ttctatcgct
     8581 tcaaattttt tcttggtttg cagatactca acattcggtc gtatttctat agtattcggc
     8641 agatttatta cagccaagca tctcaaaaat acggacagca tcctctatct ttttctgacc
     8701 ttccttgact ctaccttgct tgctatcaag gagaccttct gcccacagat aaacaattcg
     8761 gaaataggtc tcattttcct tgtagaaatg ctcttcgata acacgtttaa aataataggc
     8821 attggtaaat tcttcacact caatactagc taaaaagcca ttcaatagta tagtatgaaa
     8881 aaggtttcga ttgccagaca tttccattag aaaatcgaat ttacgtacca tttctcgtac
     8941 atatctagta aaaagagaaa cggataaaaa tggagaactg actgaaaata aattgagttc
     9001 atagattccc cagatctcgg tagaaaacaa ataatcatga aggacttttc cttcctctgc
     9061 tgttaagtct accctttcat ctatgctctt catataagac ttgatcataa tggcatttag
     9121 aatatgtttc tgtttgttgt gagaatgggc atgcttttga tactccctgc gatataagtc
     9181 ctcaagaggt gctatattct ttggttccaa gacatctgta atttcttttc tcaactcaga
     9241 atctgtatca tactggaaac ctctcgccag aaagaggatc tcctccacac tggcagatat
     9301 attttccaga gcaaatagaa acttttccac cgaaagctca ctctgacctg tttcaaaacg
     9361 ggacaacata gacggcgaaa attgtcctcc ggttgcttgt ctcagtgaga tatttcttga
     9421 ttctcgtaat tgtctaaaga cttttccaat ctgctccata gacttcccct tgattccgta
     9481 ttttcttcat tttatcatat ttttcagaaa attcatcaaa aacttgccaa attgtcagaa
     9541 ttatgagaaa atagaggata tttatcacgt ggagggactg ctatgagaga cgatatcaaa
     9601 atcaatgacc gtgctttggc cttgcaagac caaattatcg aaaaactaga gaaagttttt
     9661 gatacagatg tggaattgga tgtttacaat ctaggtctga tttatgaaat caatctggat
     9721 gaaacggggc tctgcaagat tgtcatgacc ttcaccgata ctgcctgtga ttgcgccgaa
     9781 agcctaccta ttgaaatcgt ggcaggtctg aaacaaatcg agggtatcaa agatatcaag
     9841 gttgaagtta cctggtcgcc tgcttggaaa atcacacgaa tcagtcgcta tggccgtatt
     9901 gcccttggat taccacctcg ttaagcaggc caatcacttt taaagatgaa aaaagcaagc
     9961 cgaaaatggc ttgctttttg atactcaatg aaaatcaaag ggcaaactag aaaactagcc
    10021 gaaggttgct caaaacactg ttttgaagtt atggatagaa ctgacgaagt cagctcaaaa
    10081 tactgttttg aggttgtgga tagaactgac gaagtcagct caaaatactg ttttgaggtt
    10141 gtggatagaa ctgacgaagt cagctcaaaa tactgttttg aggttgtgga tagaactgac
    10201 gaagtcagct caaaacactg ttttgaggtt gtggatagaa ctgacgaagt cagtaaccat
    10261 acctacggca aggcgacgtt gacgtgattt gaagagattt tcgagtatga gtttattttt
    10321 tacctgactt gtccatattc cagaagtctg tcacggctcc gcgtgaagca gatgatacga
    10381 tgtgggcata tttaccgagg acaccacggc tgtaaagtgg tggcaaggtt gtttctgcct
    10441 tgcgtttttc aagttcttct tcggatacag ccatggaaat ttctttggta tcttggtcaa
    10501 ccgtaacgat atcgccggta cggagatagg caattggtcc accatcctga gcttcaggag
    10561 ctatatgtcc aacaaccaga ccataagtac caccagagaa acgtccgtcc gtcaagaggg
    10621 ccaccttatc tccctgacct ttaccaacaa tcattgaaga aagtgatagc atctcaggca
    10681 taccaggacc acctttaggt ccaacaaaac gaacaacgac tacatcgcca tcaacgattt
    10741 catctgtcag aacggcctga atcgcatctt cttctgagtc aaagacctta gctggtccaa
    10801 cgtgacgacg cactttaaca cctgatacct tggcaactgc accgtcagga gcaaggttcc
    10861 cgttcaagat gataagcgga ccatccgcac gttttggatt ttcaagtggc atgataactt
    10921 tttggcctgg agtcaagtct gcaaagtcag ccaagttttc agctacagtc ttaccagtac
    10981 atgtgatgcg atctccgtga aggaaaccat ttgccaacaa gtacttcata accgcaggga
    11041 caccaccgac ttcgtagagg tcttggaaga catactgacc agatggtttc aagtcggcca
    11101 agtgaggcac acgttcttga atcgtattga agtcctcaag tgacaagtca acatttgcgg
    11161 catgggcaat ggcgagcaag tgaagagtgg cgtttgtaga accaccgaga gccatcgtta
    11221 cagtaatagc atcttcaaag gcttcacgag tcaagatatc tgatggtttg agaccaagtt
    11281 ccaacatctt aacaacagca cgtcctgctg cttcgatatc ttctttctta tcagctgatt
    11341 cagctgggtg agaggatgac cctggcaaac tcatccctag aacttcgata gcagttgcca
    11401 tggtattagc agtatacata ccaccacaac caccagggcc agggcaggca ttacattcaa
    11461 gacgtttcac gtcctcagct gtcatgtcac cgtggttcca ttttccgata ccttcaaaga
    11521 cagaaaccaa gtcgatatct ttaccatcaa gatttcccgg tgcaatagtt ccaccatagg
    11581 cgaaaatagc tgggatatcc atattagcaa tagcaatcat agatccaggc atgttcttgt
    11641 cacagccacc gatagcgacg aaggcatcca cgttgtgacc actcatagcc gcctcgatgg
    11701 agtccgcgat gatgtcacga gatgttagag agaaacgcat accaggcgtt cccatagcga
    11761 tcccgtctgc tacggtaatg gttccaaact gtacaggcca agcgcctgca gatttgacac
    11821 cttctttagc cagtttcccg aaatcatgca agtgaatgtt acatggtgta ttttccgccc
    11881 aagtcgaaat cactcccaca atcgatgttt caaagtcctt atctgtcata ccagtcgcac
    11941 gaagcatagc acggttaggt gatttaacca tgctgtcata gatgctactg cggtgacgtt
    12001 tatctaattc agtcatctta tccctcccat ttcagttttt actattatag cacaattttc
    12061 gcatgaagaa cagaataaaa atcttgaatt ttcagaaaat tctatacaca tgtgaaatat
    12121 ttaaaattaa aaacaacaaa gcggattagt gcactttctg atgaccagaa tatgcttttt
    12181 aatccgcttt ctttaaataa cgtactgtaa tttttacaga aattctttca gataagtgta
    12241 tttaacatct atcttgcatt ataaatttct agaaccttct cttttatatt cgattcactc
    12301 aaaccatact cattaagaag ataatccatt ttccctactt gaccgaatct ttcttgaaca
    12361 cccatccgat gaatttttgt tattccatca tcagagaata attcacataa agcactgcca
    12421 attccaccta tctgattgtg gttttctaca gtaaatatag tttttccact taacattgtt
    12481 tttatctgtt ctggtatcgg tttgattcta aataaatcta tcacacctac tgaataacct
    12541 aatttagaca gttcatctgc aactcgaata cttggagcaa ccattatacc agaagcaacg
    12601 attacaagat cttccccatg tcttaactca atgtagcctt tagaaaaatc ttctccacct
    12661 tgatacacag gaactggagc ttttctaatt gttcgaatat attttagtcc ttttaagtct
    12721 aatgtctggt tcaatatttc acgaaattgg atatcatcag ttgcttcaaa aatggtcgat
    12781 ttaggaatta aacgtaacaa tccaatttct tcaaatggca tatgtgtccc accattcatt
    12841 tctgcagtta ctcctgcatc tgatccaatc acagtggcat ccaattgtgc gtatccaaga
    12901 gaaataaata attgatcaaa tacccttcgt gaagcaaaag gaccaaatgt atgaagataa
    12961 ggtctaaacc cctgaataga caagcctgct gcaaggccga ccatttctgc ttccataatc
    13021 ccaacattca cataacggtc tccaaagttc ttttcaagat tatgagtagc catcgaactt
    13081 gacaaatcgg cttctaagac tactatatca gaataacttt gattagcctc taaaaggaag
    13141 tctctatata catgccgtaa ttctttcgta cttctcatca ttctgtttcc tccaattcct
    13201 gacttaatct ttctacaact gaagttaaca tttgtttctc ctctacagta gggcgaagat
    13261 gatgattgga tttcatttct tccagctctt gaaccccttg acctttaata gtatctaata
    13321 caatgcactt aggtgatgaa ttatttgact gttttaattg gacaatccct tcataaattt
    13381 ctctaatatc tgaacccttg accctaatgg attcaaatcc aaatgctgaa aatttttcta
    13441 cgaaatcacc tggattacaa atatcctttg taaaaccatc taattgtttt ttgttatcat
    13501 caacaaatac aattaagttg gataactgtt gatgagaagc aaactgtata gcctcccaac
    13561 attgtccctc atttaactca ccatctccaa caatagcata agtataaaag ggactctttc
    13621 ttattctctg accatatgca agtccagttg caacactaat tccttgtcct aaagagcccg
    13681 ttgtcatatc tatgcctggc gttagatttc tatcaggatg agacggtaat ttggttccat
    13741 ttgtatttaa agaatataag aattctttgt caaagaaacc attcaaatag agtgtactgt
    13801 atagagctgg tcctccatga ccttttgata atatgaaata atctctatct cgtgctgcaa
    13861 atatttctgg agtcattggc attatttcac cataaagcac tgctaaaact tctacgatag
    13921 acagacttcc tccgtaatgt ccgaatccaa gatgattcaa tgttctaaga gtatttaatc
    13981 ggatgttagt cgcaaatttt cttaactcat cttctctatt ttcacttaaa atcatccctt
    14041 actcctcctt tgcagatggc tttttaataa aggatactcc aaacataact gctagaataa
    14101 gaacaagacc aatcacaatg cctgcttgtg agccaaattg atttaacatt cctaaaataa
    14161 ttcctgatag accaaaatct gcatctgaga aagttgatcc ttggaaacca agtcctccca
    14221 aaactggcat taaaaagact ggaagaaaac tgattaaaat accttgtaaa aatgctccaa
    14281 tagtggctcc acgaacacca ccagatgcat tcccaatgac acctgcagtc gctccacaga
    14341 agaaatgagg cacaacacct ggtaagataa caaccgttcc tgaagcaatc ataattgcca
    14401 tacttactaa accaccaata aaactagaga taaatccaat tagaactgca ttgggtgcat
    14461 aagtataaac aatcggacaa tccaaagcag gttttgaatt aggtacaaga cgctctgaaa
    14521 tacctttaaa ggctggaaca atttcaccca aaataaggcg aacacctgct aaaataacaa
    14581 atacccctgc tgcaaattga cctgctaatt gtaaagcata aactagacca cttgtaccac
    14641 tactgatttc tttttctata tattctgacc ctgcaaagat agctacaata atgtaaataa
    14701 ctgccataga taaagtgata ctaacagtac tatcacgtaa aaaagctaaa ctctttggaa
    14761 atttaatgtc ctctgttgat tttgatttgt caccgataag gctaccagta aaaccactca
    14821 accaatatcc caaagaactg aaatgaccta aagccacctt gtcatttcca gttaattgaa
    14881 ccatatattt ttgcacaaat gctggggaaa tactcataat aataccgagt gctaatcctc
    14941 ctagtaagat gagaggcaag ctagtaaagc cagcaactga taaaatgacc gcaattatac
    15001 atgccatata tagagtgtgg tgccctgtta aaaagatata tttaaatcga gtaaaacgag
    15061 cgattaagat attgaacacc atgcctgcaa acataatcat tgcagtagct gagccatatg
    15121 ttgttaaagc tacagctaca attgcttcat tattcggcac aacgccagat aaatgaaaag
    15181 catgctcaaa catggtacca aatggattta aagaattttg tacaattcct gcaccaccag
    15241 atacaactaa gaaaccaaca aaggtcttaa ttccaccttt aataatatca ggtaatttct
    15301 tcttctgaag aactaatcct aaaattgcaa ttaaagctac taaaatagct ggtgtactaa
    15361 caatatccaa tatgaacttc atcatgacgc tagcctccta tataagtcct ttttcttcac
    15421 aaagtttagt aattaattct cgtagttcat ccatatcaat aatactattt aagatacgaa
    15481 catctccaag atgactagct gaatcagcta gatcacgacc aacaatccaa atatcagcag
    15541 catttggatc tgctccacct aaatcataat gttcaacttc tacatccgaa acattcaaat
    15601 cactcaatac agattcaata ttcatctgta ccataaaact tgaacctaat cctgaaccac
    15661 aagctgtacc aatttttaac attatctaat cctcctgttt aattatcatt ttaatgtcat
    15721 catagttttt tgatgaaatt aaagtttgaa catgattttt atctcttaaa attgttgtta
    15781 aatgtgacaa agcctttaaa tgactctcat tatcaatggc tgcaatacaa atcaacaatc
    15841 ttacctcttg ttctggatta tccaataaat aaatcggttc ttccaaaact aacattgaca
    15901 ttcctatttc attcacacct tcatctggcc gagcgtgagg aattgctact cccttcccta
    15961 aattaataaa aggtccaaac tcttctactt tttgaatcat tgcctcaggg tagttctccg
    16021 ttatcttatc ttgatccaaa agcggtttag ctgctaaacg aatcgcctcc ttccatccta
    16081 atttttgcga actaacctga taggtttctt tggtaataag ttgttctagc actggtacaa
    16141 tttcctttct atcatttttt tgataaagat aattctttaa cgccaatctt aattccaatt
    16201 cttgtgtaat aattccatat cttttgacaa tattcaggat ttgttcaatc tcaaaatctc
    16261 catactctaa attcggaaaa tcttttaaca ctagttctac tagttgtatt gcttgctctt
    16321 cagtcatcat aaccgaaact agataatttg gcttttctgt ctccaccttt atggtagaaa
    16381 aaaccatatc atagtcacta ctagctttca cctgtaaatc atcaatcttt gaggttccta
    16441 taaactcaat ttgaggaaat aatgctaata gattctcttt taacatcaat gaagaactaa
    16501 caccattagg acaaatgatt gctgctttat accatttttg aggcaaagta tctgctttct
    16561 ttaaataacc tccgaaatgg ataacaaaat atgctgtttc actatcaggt atgggattgt
    16621 caatagcgtc catcaagggc atcaaagaat ctttgactaa ttcaaataaa tcaggataat
    16681 gttctttaac atgcaataca tattcatttg aactaggtag gccgaacttt aatctatagt
    16741 aagccggtat aaggtggcgg cgaagatttt ctctcaatcc ttccctttgt ttaaaatgta
    16801 acaaagaaat atcttccatt ctacttataa tagcctctgt taattgatta aagtaaaccg
    16861 gagcaacatc tacttcacct tcaaagcaac ttgataataa aactgtgata tagcgataat
    16921 catcctcaga aaacaccgta tctataattc ccaaatcaac cactgtatcc aataaaatag
    16981 tggttatatc ttgaataaca ggagatacta atgtctctga aagacatact ctttcaacat
    17041 ccctttgata cctacacaga atgaatacta aaccgaaaag gcaaactttt aattgattaa
    17101 caataggtac tagctgtagc ttctcataat aatctttaac tacctgattt atcaaatcat
    17161 aagttaatga atacccccaa ctggataaaa cataatccaa accccaaatc cctatggagg
    17221 attccagcaa ctcactaacc atttgaaaag ctaaacggtg cttattccac tctgaaccgt
    17281 gtaaagtata accttttgct ctactgtacc ctagctccaa atcattatct aacataatct
    17341 ttcttaatga ttgaatatca gttaaggttg tattcttact tactttcaaa aagtcttggt
    17401 aatgattatt cgatataaaa tctaatcggc aaaaagtgta aagatagatt aaagctaagc
    17461 gagtcgactt tggtaaaacc aattcatccg acttaataat atctgtcaaa gactgcttcg
    17521 tacgatttga taaactatag cgaccttgtt ttttatccag cactatccct ttattagcta
    17581 gataaggcac taaataatct attccttctt tgacttcctt tataggtaag ctcaccttaa
    17641 cagataattc atataatgat agctcacaat gatccatcaa agtcatcaaa ataactagtg
    17701 ctctataatc aaacatttct cttcctttct aactacaatt atagtgtaaa aattataaga
    17761 ataaaagagt atttgagcac aatataatct caccttttaa aagaaattat tgggctgaaa
    17821 gtttttagtg attatttttc aactttctat tgactttata aattaaagtg atatcattta
    17881 gatatcaaat aacaggaggt cattcttatg actgaaaaac taatcaattc aaaaccaaat
    17941 ggtgtattcg cattgattct cattgagttg acaatcgtac ttggtatctt tatatttata
    18001 atgggggttg gttcggaaaa catttttgga attattatcg gacctttact aatcgtaatt
    18061 gcaggtctag ctcatgctgg tttaaaagtt gtcaaacctc aggaagctct ggttctgaca
    18121 ctctttggta attatacagg taccatcaaa gaacctggtt tttactttgt caatcccttc
    18181 agcgtagcag tcaaccctgc aaaccacact cgacttggac aaagtggtga tgttagcaca
    18241 aaatctcctt ttttaggagc taaatcatca aatggcaatg atgtaaatct tgaaattggc
    18301 aagaaacaca tttccctcaa agtcatgacc ttgagcaatt ctcgtcaaaa aatcaatgat
    18361 tgcttaggaa accctgtaga aatcggtatc gcggtaactt ggagagttgt cgataccgct
    18421 aaggcagtct tcaacgttga taactacaaa gaatatcttt cattgcaatg tgatagcgcc
    18481 ctccgtaata ttgtccgcat ctatccttac gatgtgtctc ctaatgtgga tactacgggt
    18541 gacgggcaag cagatgaagg tagtctccgt ggctctagtg aaattgtagc taaccgtatt
    18601 cgtgaagaaa tccaaagtcg tgttgaggat gctggcttgg aaatccttga agcacgtatc
    18661 acttacctag cttatgctcc agaaattgct gccgttatgc ttcaacgcca acaagcatct
    18721 gccattattg atgcacggaa gatgattgta gatggtgctg taggaatggt tgaaatggca
    18781 ctagaacggc tcaatgaagg ggaattggta gaacttgacg aagaacgaaa agctgccatg
    18841 gtttcaaatc tccttgtcgt tctttgtggc aatcatgatg cacaaccaat tgtcaacaca
    18901 ggaagtcttt actaaagatg gctgaagcta aaaaaaagca gatccccctt cgactctcaa
    18961 caaagttata cgctgcactc gcatcatggg cagaagacga ctttcgttca gtcaatgggc
    19021 aaatcgaata tctcctcaca gagtgtgtca agcaacggaa aaaagacgga aaatacgtat
    19081 cagaaaccat tgacgaacca tttgagattg atatttaaca ccttctcctg tctgccgtgt
    19141 cggataggag aattttttca tctttttttg tcaagtaact ttactttaca aaaaaaatgt
    19201 gttatcctag tatggttgat gaaaatcagt agattgaatc gaatataaat acctaaagga
    19261 gaaatcaaaa tggcagtacc tgcacgtcgc acttcaaaag cgaagaaaaa caaacgtcgt
    19321 acacactaca aagtaacagc tccatctgta aactttgacg aaactactgg agattactca
    19381 cgttctcacc gtgtatcact taaaggatac tacaaaggac gtaaaatcgc taaagctgca
    19441 tcagctgaat aatagaaggg agataccatg cgcgtaaata ttacacttga acacaaagaa
    19501 tctggtgaac gcttgtacct tacttctaaa aacaaacgta acactccaga ccgccttcaa
    19561 ttgaagaaat actcaccaaa acttcgcaaa cacgttgtgt ttacagaagt gaagtaagca
    19621 ttcaatacga atcaatacag aagaaaaacg cttgatcagc gttttttctt tttgttaatt
    19681 tcactatatt gcaagagaaa tcattcgaat gactacattt tttgaaataa gaatacggat
    19741 tcggacgatt aaaagtaaga gaaactcaaa tgtaagttgg ggttcctttt ttgtatattt
    19801 ttcaaataac gcctctacac tatttgtagc aaattcacca actacagttg catcttagtt
    19861 aaaataagtt agaatatgta agtgagtacc agatatacca agacatcgtc accatctaag
    19921 gtatattcaa aatacaaaag ttgaccaact agatttctga atatccttat atatccattc
    19981 ttaaaattgg tttaaatagc gtagtctttt aaactagttt tgagaatcca aaaaatcttc
    20041 ctacatatgt aagaagattt tttagttcag aatgattaga tttagctaat ggatacctat
    20101 cctacccact caccgttagc attgactcga tagccatcgg gtgtagtcgt gtttactaat
    20161 aaatctcctg aattataggt atagtaccat ttaccagaaa ctgtaaacca accag
//
LOCUS       DAVKWZ010000036        18678 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000036 DAVKWZ010000000
VERSION     DAVKWZ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18678
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.036"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            83..547
                     /locus_tag="VW854_001372"
     CDS             83..547
                     /locus_tag="VW854_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEV0262596.1"
                     /translation="MGGHPGFNCLLLDDEVYEDYYLEFEKEEICSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSTDAVTLDELQSRTIALRSLKHDKGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            704..1129
                     /gene="lacA"
                     /locus_tag="VW854_001373"
     CDS             704..1129
                     /gene="lacA"
                     /locus_tag="VW854_001373"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEV0262597.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            1160..1675
                     /gene="lacB"
                     /locus_tag="VW854_001374"
     CDS             1160..1675
                     /gene="lacB"
                     /locus_tag="VW854_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEV0262598.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGIRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHLESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            1686..2615
                     /locus_tag="VW854_001375"
     CDS             1686..2615
                     /locus_tag="VW854_001375"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEV0262599.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLTKA
                     NVLGMLNAQEKMTGHVNMANYQVLYDQLIVKEV"
     gene            2617..3597
                     /gene="lacD"
                     /locus_tag="VW854_001376"
     CDS             2617..3597
                     /gene="lacD"
                     /locus_tag="VW854_001376"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEV0262600.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            3831..4667
                     /locus_tag="VW854_001377"
     CDS             3831..4667
                     /locus_tag="VW854_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV0262601.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            4703..5020
                     /locus_tag="VW854_001378"
     CDS             4703..5020
                     /locus_tag="VW854_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV0262602.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            5020..6711
                     /locus_tag="VW854_001379"
     CDS             5020..6711
                     /locus_tag="VW854_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV0262603.1"
                     /translation="MNKLIAFIEKGKPFFEKLSRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALLVAGTTAKSLTDSVNRSMEKTNQ
                     INYMSTLLAAIVGLLMLAADPIESGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
                     TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARHFVGASVAESIGKFFAPLFSA
                     ADGYLGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLV
                     LNPIFFIPFIFAPIANVWIFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFI
                     LAALLIVVDVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKAG
                     VDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLPE
                     FDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQAQFD"
     gene            6920..8326
                     /gene="lacG"
                     /locus_tag="VW854_001380"
     CDS             6920..8326
                     /gene="lacG"
                     /locus_tag="VW854_001380"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEV0262604.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPLDHENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGKKGSSKYQIKGVGRR
                     VAPDYVPRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            8461..8625
                     /locus_tag="VW854_001381"
     CDS             8461..8625
                     /locus_tag="VW854_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEV0262605.1"
                     /translation="MESLGKWYVSTGKEWICHSDDELEEFKNLFLNFINPEEWDTISF
                     DSDFMPFQQS"
     gene            complement(8904..9665)
                     /locus_tag="VW854_001382"
     CDS             complement(8904..9665)
                     /locus_tag="VW854_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV0262606.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNISNLDTIVSDSKLSDSILFKLSKHIKVIKP"
     gene            complement(9847..10809)
                     /gene="nrdF"
                     /locus_tag="VW854_001383"
     CDS             complement(9847..10809)
                     /gene="nrdF"
                     /locus_tag="VW854_001383"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEV0262607.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            complement(10997..13156)
                     /gene="nrdE"
                     /locus_tag="VW854_001384"
     CDS             complement(10997..13156)
                     /gene="nrdE"
                     /locus_tag="VW854_001384"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEV0262608.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDDQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(13237..13455)
                     /gene="nrdH"
                     /locus_tag="VW854_001385"
     CDS             complement(13237..13455)
                     /gene="nrdH"
                     /locus_tag="VW854_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEV0262609.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            13806..14069
                     /locus_tag="VW854_001386"
     CDS             13806..14069
                     /locus_tag="VW854_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEV0262610.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            14075..15808
                     /gene="ptsP"
                     /locus_tag="VW854_001387"
     CDS             14075..15808
                     /gene="ptsP"
                     /locus_tag="VW854_001387"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEV0262611.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            16063..18513
                     /gene="phtA"
                     /locus_tag="VW854_001388"
     CDS             16063..18513
                     /gene="phtA"
                     /locus_tag="VW854_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HEV0262612.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPHGDHYHF
                     IPYSQMSELEERIARIIPLRYRSNHWVPDSRPEQPSPQPTPEPSPGPQPAPNLKIDSN
                     SSLVSQLVRKVGEGYVFEEKGISRYVFAKDLPSETVKNLESKLSKQESVSHTLTAKKE
                     NVAPRDQEFYDKAYNLLTEAHKALFENKGRNSDFQALDKLLERLNDESTNKEKLVDDL
                     LAFLAPITHPERLGKPNSQIEYTEDEVRIAQLADKYTTSDGYIFDEHDIISDEGDAYV
                     TPHMGHSHWIGKDSLSDKEKVAAQAYTKEKGILPPSPDADVKANPTGDSAAAIYNRVK
                     GEKRIPLVRLPYMVEHTVEVKNGNLIIPHKDHYHNIKFAWFDDHTYKAPNGYTLEDLF
                     ATIKYYVEHPDERPHSNDGWGNASEHVLGKKDHSEDPNKNFKADEEPVEETPAEPEVP
                     QVETEKVEAQLKEAEVLLAKVTDSSLKANATETLAGLRNNLTLQIMDNNSIMAEAEKL
                     LALLKGSNPSSVSKEKIN"
BASE COUNT         5917 a         3381 c         3835 g         5545 t
ORIGIN      
        1 tgctggtatt atcaatcata aactagaatt ctagttttgc aggaagtcta ataaagaaac
       61 tagcaaggtc aggccttact ttattggtgg acatccagga tttaattgtc ttctacttga
      121 tgatgaagtc tatgaagatt attatctaga gtttgagaaa gaagagattt gctctgttcc
      181 acgtcctttc ccagaaacag gtatgttgga tttccaagat agaagtccat ggctagaggg
      241 acaaaaagaa atagatctta gttatgatct tttcagcaca gatgcagtga ctttggatga
      301 attgcaatct cgaacaattg cccttcgttc tcttaaacat gataagggat tgaaagtgca
      361 ttttgcagaa tttccaaacc tcatcatctg gtcaactttg aacaagggac ctttcattac
      421 ctttgaacca tggtctggct tgtcaacatt ccttgaagaa ggagatcatt tagaagataa
      481 gaagaatgtt tgtctcttag aagccaatca ggttgaagaa ttagggtttg agatagaagt
      541 tttataaaaa agcaaaaaat aaataacaaa aacaaacaca aactgttgac ttcttcaaac
      601 aatagtagta tattatgttt gtgaggacta attgactgtt ttttaaagac agccaataca
      661 ctgctggaat ccagcataga aaaaataaaa aggagtatac aatatgtcta ttgttatcgg
      721 tgcagatgct gcaggtttga gattgaaaga agttgtgaaa gacttcttag aaaaagaaaa
      781 cttccacctt gtggatgtta cagctgaagg tcaagacttt gttgatgtga ctctcgctgt
      841 tgctgcagaa gtaaacaaag aagaacaaaa ccttggtatc gtgattgatg cttatggagc
      901 tggtccattt atggttgcaa ctaagatcaa aggaatggtt gctgcagaag tatctgacga
      961 acgttcagct tatatgactc gtggccacaa caactcacgt atgatcacta tgggggcaca
     1021 acttgttggt gatgaattgg ctaaaaatat cgctaaagga tttgttaacg gtaaatacga
     1081 cggtggtcgt caccaaatta gggtcgacat gttgaacaaa atgggctaat tagattatag
     1141 taagaaaggt aagttaaaaa tgagaattgc aattggatgt gaccacatcg taacagatga
     1201 aaaaatggcg gtttcagaat ttttgaaatc aaaaggatat gaagtcattg actttggtac
     1261 ctatgaccat acacgtactc actacccaat ctttggtaaa aaagtagggg aagcagtaac
     1321 tagtggtcaa gctgatctcg gggtatgtat ctgtggtact ggtgttggta tcaacaacgc
     1381 tgtaaataaa gttccaggta ttcgttctgc cttggttcgt gatatgacaa cagcccttta
     1441 tgctaaagaa caattgaacg ccaacgttat cggttttggt ggtaagatta ctggtgaatt
     1501 gcttatgtgt gatatcatcg aagctttcat tcatgctgaa tacaaaccat ctgaagaaaa
     1561 caaaaaattg attgcgaaaa ttgaacacct tgaaagtcac aatgctcaac aaacagacgc
     1621 aaacttcttc actgaattcc ttgaaaaatg ggatcgcgga gaataccacg actaagaggt
     1681 aacctatgat tttaacagtt acaatgaacc catccattga tatttcctat cccttggatg
     1741 agttgaagat tgatactgtc aatcgtgtgg tggatgtaac caaaacggct ggtggtaagg
     1801 gactcaatgt tacccgagta ctttcagaat ttggcgattc tgttcttgct actggtttag
     1861 tgggtggcaa acttggtgag tttttggttg aacatatcga taatcaagta aagaaagatt
     1921 tcttctcaat tcagggagaa actcgtaact gtatcgctat tctccacgga gacaaccaaa
     1981 cagaagttct tgaaaaaggt cctgaagtat tggaacagga aggtcaagac tttttggaac
     2041 atttcaaaaa actcttggag tcagttgaag tagtggccat ctcaggtagt ctgccagctg
     2101 gccttccagt tgattactat gcgagcttgg tagaacttgc taatcaagct ggcaagcctg
     2161 tagtcttgga ctgctcaggt gcagcacttc aggctgttct tgaatcaccc cataaaccaa
     2221 cagtcatcaa accaaataat gaagaattgt ctcagcttct tggaagagaa gtttctgagg
     2281 atttggatga attaaaagaa gtacttcaag aacctttgtt tgcagggatt gaatggatta
     2341 tcgtttcact tggtgccaac ggtacttttg ccaaacatgg tgacactttc tacaaggtag
     2401 atattcctag aattcaggtg gtaaatcctg ttggatctgg agactctact gtggcaggaa
     2461 tttcttcagg acttcttcac aaagaatcgg atgcagaatt actcaccaag gcaaatgtcc
     2521 ttggtatgct caatgctcaa gaaaaaatga ctggtcatgt caacatggcc aactatcaag
     2581 ttctatatga tcaattaata gtaaaagagg tataaaatgg ctttaacaga acaaaaacgt
     2641 gtacgcttag aaaaactttc tgatgaaaat ggtatcatct cagctcttgc atttgaccaa
     2701 cgtggtgctt tgaaacgcct catggctcaa caccaaacag aagaaccaac tgtggcccaa
     2761 atggaagaac ttaaagtctt ggtagcagat gaattgacta aatacgcttc atctatgctt
     2821 cttgaccctg agtatggact tccagcaact aaagctcttg atgaaaaagc tggtcttctc
     2881 cttgcttatg aaaaaacagg ttatgacaca acaagcacaa aacgcttgcc agactgcttg
     2941 gatgtctggt ctgcaaaacg tattaaagaa gaaggtgcag atgcagttaa attcttgctt
     3001 tactatgatg tagatagctc agacgaactc aatcaagaaa aacaagccta catcgaacgc
     3061 atcggttctg agtgtgtggc tgaagatatc ccattcttcc ttgaaattct tgcttacgat
     3121 gaaaaaattg cggatgcagg ttctgtagaa tacgcgaaag taaaaccaca caaagttatc
     3181 ggcgctatga aagtcttttc agacccacgc tttaacattg atgtcttgaa agttgaagtt
     3241 cctgttaaca ttaaatatgt tgaaggcttc gctgaaggtg aagtagttta tacacgtgaa
     3301 gaagcagcag ccttcttcaa agcgcaagat gaagcaacga acttgccata catctacttg
     3361 agtgctggtg tatcagctaa actcttccaa gatactcttg tatttgctca tgaatcaggt
     3421 gcaaacttta acggagttct ttgtggccgt gctacatggg cagggtcagt tgaagcttac
     3481 atcaaagatg gtgaagcagc agctcgcgaa tggcttcgca caactggatt tgaaaacatt
     3541 gatgagctca ataaagttct tcaaacaaca gcgacttcat ggaaagaacg tgtgtaagaa
     3601 agtcctccta gtttaggaac atgaatctaa aaaaatttaa aaaaagttgt atgtaaaggt
     3661 ttacaaaata acttacttgt gctatactta aatcacaagt taatacaagg tgagtgttac
     3721 taagtaatat taggcatgat cacaggtgaa ttagaaatca gctgattttc tagttcattt
     3781 gtggtcattt tttgtactta tataccttta agatataaaa ggaggttgac atgtatcgaa
     3841 ttctaaatcc aatgaatcac aatgtctcgc ttgtcagaaa tgataaggga gaagaggtga
     3901 ttgtaattgg taaggggatt gcattcggaa agaagaaggg ggatttgatt gctgaaaatc
     3961 aggttgagaa aatctttcgg atgaagaccg aagagtccag agaaaacttt atggctcttc
     4021 tcaaagatgt tccgcttgat tttatcacag tgacctatga aatcattgat aagctatcaa
     4081 agaaatatca ttatccgatt caagagtatc tctatgtaac cttgacagat catatttact
     4141 gttcttatca agctctaact caaggaaggt acaaggatag taatctgcca gatatttccg
     4201 ctaagtatcc tgtcgctttt caaatcgcaa atgaagcttt tgaaatttac cgtcagaagc
     4261 tagcagatca ttttcctgag gacgaaatta ttcggattgc ttatcatttc attaatgctg
     4321 aaggtgaaaa tgaagtggaa cttgtggagt cgattgataa gaggaaagaa attctcagga
     4381 atgttgaaga agttttaacg gactatgcaa ttcaacgaac taaaaagaat aaccatttct
     4441 atgatcgctt tatgatccat ttgaattatt tcttggatta tttagacaga tctagagatg
     4501 ataaccaatc acttctggat atggaagatc atattaaaca atcctatcca aaagccttcg
     4561 agattggttc caagatctat gatgtgatta cgcaacatac gggtcttgat ttgtataaaa
     4621 gtgaacgagt ttatctagtt ctacatatcc aacgtttatt gtcataaaaa tttatttaaa
     4681 actatataag gagaattcta tcatgaatag agaagaagta acattgttag gttttgaaat
     4741 cgtagcctat gctggcgatg ctcgttcaaa actattggaa gccttgaagg ctgctgaagc
     4801 tggtgatttt gaaaaagcgg acgctctggt agaggaagct ggtagctgta ttgcagaggc
     4861 tcaccacgcg caaacaagtc tattgactaa ggaagcttca ggtgaggact tggcttatag
     4921 tgtaaccatg atgcatggcc aagaccactt aatgacaact atcttgttaa aagatttgat
     4981 gcatcattta attgaactct acaagagagg agttaaataa tgaataaact aattgcattt
     5041 atcgagaaag gaaagccttt ctttgaaaaa ctatctcgta atatctatct tcgtgctatt
     5101 cgtgatggtt tcattgcagg tatgcctgtt attctcttct caagtatctt tatcttgatt
     5161 gcctttgtac caaactcatg gggctttaaa tggtctgatg aagttgtagc ctttctgatg
     5221 aaaccttata gctattctat gggtattctg gctctcttgg tagctggtac aacagctaag
     5281 tcattgactg actcagtaaa ccgtagcatg gaaaaaacca atcaaatcaa ctatatgtca
     5341 acattgttgg cagcaattgt tggtttgttg atgttggcag ctgatcctat cgaaagtggt
     5401 ctagctactg gattcttggg gacaaaaggt ttgctttcag ccttccttgc tgccttcgtt
     5461 actgtagcca tctataaggt ttgtgttaag aacaacgtca ctattcgtat gcctgacgaa
     5521 gttccaccaa atatctcaca agtctttaaa gatgtgattc cattcactct ctcagttgtt
     5581 tcactttatg ctcttgattt actagctcgt cattttgttg gtgcaagcgt agctgaatca
     5641 atcggtaaat tctttgcacc actcttctca gcagcagacg gataccttgg tattaccatt
     5701 atctttggtg cctttgcctt cttctggttt gtgggtattc atggtccatc tattgttgaa
     5761 cctgctatcg cagcgattac ctatgcaaat gctgaagtca acttgaacct tctccaacaa
     5821 ggtatgcatg ctgataaaat ccttacttca ggaactcaaa tgtttatcgt tactatggga
     5881 ggtactggtg cgacactggt tgttccattc atgttcatgt ggttaacaaa atcaaaacgt
     5941 aaccgtgcaa tcggacgtgc ttcagtagtt ccaactttct ttggtgtaaa tgaaccaatc
     6001 ttgtttggtg caccacttgt attaaatcca atcttcttca ttccatttat ctttgctcca
     6061 atcgcaaacg tatggatttt caaattcttt attgaaactc ttggaatgaa ctcattcact
     6121 gctaatctac catggacaac tccagctcca ctaggtctag ttcttggaac taacttccaa
     6181 gtgctatcat tcattcttgc tgcccttcta atcgtggttg acgttgtcat ttactatcca
     6241 ttccttaagg tctatgatga acaaattctt gaagaagaac gttcaggtaa gtctaatgat
     6301 gaattgaaag aaaaagttgc tgcaaacttc aacactgcaa aagcggatgc tattcttgaa
     6361 aaagcgggtg tcgatgcagc acaaaatacc atcactgaag aaacaaatgt cctcgttctc
     6421 tgtgcaggtg gaggaacaag tggtctcctt gcaaatgctt tgaataaggc agcagcagaa
     6481 tacaatgtcc ctgtgaaagc agcagcaggc ggctatggtg ctcaccgtga aatgttacca
     6541 gagtttgatc ttgttatcct tgcccctcaa gttgcttcaa actttgaaga tatgaaagca
     6601 gaaacagata agctcggtat taaactagcg aaaacagaag gcgctcaata catcaaatta
     6661 actcgtgatg gaaaaggtgc tcttgcattc gtacaagcgc aattcgatta aggctagaga
     6721 ctctgaaata gtctcctatc gttacggaaa tcgctatggc gaatttccta ttattaattc
     6781 gtcggtaaaa agatatcgtt tttacctcct catgtcacaa ttcggtgact tggtacaaga
     6841 agtgagatgg agaaggatgg ctcactgact cctctcctct cacttttact ttatttaaat
     6901 caagaaatag gtgaaaaaaa tgacaaaaac acttccaaaa gactttattt ttggtggcgc
     6961 aacagctgct tatcaagcag aaggtgctac acatactgat ggaaaaggac cagttgcttg
     7021 ggataaatat cttgaggata actactggta cactgccgaa ccagctagtg atttttacaa
     7081 tcgatatcca gttgacctca agctagcaga agagtatggt gtcaatggta ttcgaatttc
     7141 tattgcttgg tcacgtattt tcccgactgg ttacggccaa gtaaatgcta aaggtgttga
     7201 gttttatcat aatttatttg cagagtgtca caaacgtcat gttgagcctt ttgtaactct
     7261 tcatcacttt gacacgccag aagctctcca ctcaaatgga gacttcttaa accgtgaaaa
     7321 tatcgaacat tttgtagact acgctgcctt ctgttttgaa gaatttccag aagtaaacta
     7381 ttggacaacc tttaatgaaa ttggaccaat cggtgatggt caatatttag ttggtaaatt
     7441 ccctccagga attcagtacg accttgccaa agtcttccaa tcgcaccaca atatgatggt
     7501 gtctcatgca cgtgctgtaa agctatataa agataaagga tataaagggg aaattggtgt
     7561 tgttcacgcc ctgcctacta aatatcctct agatcatgaa aatccagcag atgttcgtgc
     7621 agctgagttg gaagatatca tccacaataa attcatctta gacgcaactt atctaggtcg
     7681 ctattcagct gaaaccatgg aaggtgtcaa ccatatctta tcagtcaatg gtggtagttt
     7741 ggatcttcgt gaagaagatt ttacagcatt agaagctgca aaagacttga atgatttcct
     7801 aggaatcaac tactatatga gtgactggat ggaagccttt gatggagaaa ctgaaattat
     7861 ccataatggt aaaggtaaaa aaggaagctc taagtatcaa atcaaaggtg ttggtcgtcg
     7921 tgtagctcct gactatgtac cacgcacgga ttgggattgg attatctacc ctcaaggttt
     7981 gtatgaccaa atcatgcgtg tgaagaaaga ttatcctaac tacaagaaaa tttacatcac
     8041 tgaaaatggt ctcggctata aagatgagtt cgtcgataac actgtttacg atgatggtcg
     8101 tattgattac gtgaaacaac acttggagat cttgtctgat gcgattgcag atggagctaa
     8161 tgtaaaaggt tacttcattt ggtcattaat ggatgtcttc tcatggtcaa acggttatga
     8221 gaaacgttat ggtctcttct acgtagattt tgaaactcaa gaacgttatc ctaagaaatc
     8281 agctcactgg tacaagaaag tagcggaaac tcagattata gactagtaga attagtcatt
     8341 agatatagaa ttttagtgag tccaaaagat gttcaaagat tttatccaat ctatttatga
     8401 aaaagtttat attataaatt tcgaaaaatg ctctcaaata ccgtgtttga cgagtgaaga
     8461 attgaaagtc ttggaaaatg gtatgtctcg actggtaaag aatggatttg tcattcagat
     8521 gatgagctgg aagaatttaa aaatctattt ttaaatttta tcaatcctga agaatgggat
     8581 actatctcct ttgattcaga ttttatgccg tttcaacaat cgtaaccaat ttctcaaaaa
     8641 agttaaatct tatatttagt actctgtaaa actcttatct aatcacgttg cttatactca
     8701 atgaaaatca aagagcaact ttaaactagg aagcgagtcg cagatttctc aatgtatagc
     8761 tttgaggaat tgggcaaaaa gtctttgata tagaaaaaag catagtatca ggtgtttcaa
     8821 cacctgatac tatgcttttt attgtgggaa gatttacttt ttttcttctg aaattgagtt
     8881 gttacccagg ctctttcagt ttattaaggc ttgatgactt taatgtgttt agatagctta
     8941 aaaaggattg aatcacttag tttagaatct gaaacaatag tatcaagatt tgatatatta
     9001 taaaaagtat aaaaatcaaa cttattgaac ttactatgat ctgcgagtaa atatttttta
     9061 ttagaattat ttaaagcgat gcgttgagcc tctccctctt cctcgctaaa agtagctaga
     9121 gctccgtttt gaataccatt acagctaacg aaagctttag aaaattggag attagagaga
     9181 ttttgtaggg tcaatgtgcc aacaaaagca cctgtaatat cgcgataatt tccacctatc
     9241 aaaatcaaat ctgttaattt tcgttcgctt aaaatcagaa aaacaggtag actgttggtt
     9301 acgacgcgga tattgtcaat aggcaactca cgcgcaaaaa actctaatgt tgttcctggt
     9361 ccaatgaaaa tagtttctct ttcttctact agactgcctg caaaatgggc tatttcttgt
     9421 ttttctgccg tttggagggc ttgtttttca atatttgatc gctcattagt caaaagggag
     9481 ttggttcgaa gtttttcagc tccaccatgc acacgaatca gcaaatcttt atcagctaat
     9541 tcctgtaaat agcgccttgc agtcatatct gaaacggcta tttcgtccat aatctgttta
     9601 actgttatgg ttcctttact atttactatc tctaaaattt tggttaattt ttcttgtttg
     9661 agcatattat cacctcgttt cctactacta tcttaccata aacaaactca tcattcaaat
     9721 acaaaaacaa caaaatgaaa caaaaacaaa aatatcgaag tttgttttca aaactttcga
     9781 tatttttgtt gggttataac tttgatgttt ctagtttact ttttgatgat tgagagtgat
     9841 ggagaattag tctaaaccgt agttatagtc atcgtcttgc atggcttcaa cttcgccaag
     9901 aaggtaacca tttccgactt gagagaagaa gtcatggttg gaagttcctg ttgaaatacc
     9961 gttcataacg attgggttga catcttcagc tgaatctggg aaaagtggat cttgtcccat
    10021 gttcatgaga gctttattgg cattgtagcg aaggaaggtt ttaacctctt cagtccaacc
    10081 aacaccgtca taaagactct ctgtgtagcc ttcttcattt tcataaagag tatagagtag
    10141 gtcgtacatc cattctttga gtttttcttg ctcttcttca ggcaattcat tgaaaccaag
    10201 ttggaatttg taaccaatgt aggttccgtg aacagactcg tcacgaataa tcaatttaat
    10261 gatttctgca acgttggcta gtttgttgtt accgagatag tagaggggag tgaagaaacc
    10321 agagtagaag aggaaggttt cgaggaagac gctggcaact ttcttttcaa gtgggctgcc
    10381 gtttaggtag atttcgttga caatctcagc cttcttttgt aggtaaggat tggtattggt
    10441 ccattcgaaa atttcttcaa tctcagcctt agtattcaag gtagaaaaga ttgatgagta
    10501 agatttagcg tggacagatt ccataaattg gatgttattg aaaacagctt cctcatgtgg
    10561 tgtacggatg tctgcgcgaa gggcttgaac cccagtttca gattgcatag tgtcaagaag
    10621 ggttaaacca ccaaaaactt ttccgaccaa gtctttctct ttgttagata gctttctcca
    10681 gtcatccaag tcgtttgata agggaatacg tgtatcgagc caaaattgct ccgtcagttt
    10741 ttcccaagtt gatttgtcga tgacatcttc gatggcattc cagttaatgg ctttgtagta
    10801 agtttccatt taaaatctct ttctgtgttt agtattgcga actcacaatt atttctactt
    10861 taccataatt ctataggagt atcgcacaaa aagtcggaag cccgactttt aaaatgttac
    10921 ataaattatg ttatgacata gtagatttga ttttatctgt gctgcttagg gaaaaataat
    10981 gtttctatgc tagaaactaa atcacacagc tttcacattg gttggcgccg acttctccac
    11041 cgtcatctgt aaaggtacgg acgtagtaga tagacttgat tcccttgtta aaggcatagt
    11101 tacgaaggat ggacaagtca cgtgtcgttt gtttattttc cctcttccat tcgtaaaggc
    11161 cttttggaat gtcactacgc atgaagaggg tgagtgaaag tccttgatcc acgtgttcag
    11221 tcgcagcagc gtaaacatcg atgactttac gcatatccat atcgtaggca gaagtgtagt
    11281 aaggaatggt ttctgtagac aagccagcag cagggtagta gattttacca attttcttct
    11341 cttggcgttc ttcgatacgt tgcgtaatcg ggtggataga agcagaaacg tcgttgatat
    11401 agctgataga accatttggc gctacagcaa ggcgattttg gtggtaaaga ccatcttctt
    11461 gaaccttgtc gcgaagttca gcccaatcag caacaccagg gataaagaca tttttgaaga
    11521 gttctttaac acggtctgat gttggaacaa attcaccagt tacatacttg tcaaagtaac
    11581 ttccgttagc atagtctgat ttttcaaagt tgtggaaggt aataccacgt tcacgcgcaa
    11641 tattgtttga ctctaccaag gtccaatagt tcataagcat aaagtagatg cttgtaaatt
    11701 caacagactc aggtgaacca tattcaatga gttgttgggc aaggtagctg tgcagtccca
    11761 tggcaccgag accaaaggtg tgggcttggc tatttccatg gtcaatcgtt ggtacagcta
    11821 cgatatgtga actatctgta acgaaagtaa gggcacgaac catagcacgg atagaacgac
    11881 caaaatcagg tgaagtcatc atgttaacca cgttggttga acccaggtta catgaaacat
    11941 ctgttcccat ttgaaggaat tcttgatcat cgttgatcaa gcttggttct tgaacttgaa
    12001 gaatctcaga acacaagtta ctcatgataa tctttccatc aacaggattt gcacggttag
    12061 ccgtatctat gttgactaca taaggatagc cagactcttg ttgcaattta gagatttcag
    12121 tttccaaatc ccgcgccttg atttttgtct tgcgaatatt tggatttgcg accaattcat
    12181 cgtatttttc agtaatgtcg atgtaattga atggcacacc gtattctttt tctacagagt
    12241 aagggctgaa gaggtacatt tcttcatttt tacgagccaa ttcgtagaat ttatcaggta
    12301 ctacaacacc aagtgataga gtcttgacac gtactttttc atcagcgttt tctttcttag
    12361 ttgaaaggaa agcgatgata tctgggtgaa agacgttgag gtagacaaca ccggcacctt
    12421 gacgttgccc caattggttg gagtaagaga agctgtcttc aaaaagcttc ataacaggaa
    12481 cgacacctga agcagctcct tcatagcctt tgataggtgc accagcttca cgaaggttgc
    12541 tgagagtaat tcccacacca ccaccaatac gtgaaagttg aagagctgag ttgatagaac
    12601 gtccgataga gttcatatca tccgtcactt ggattaggaa acaagatacc aactccccac
    12661 gacgagcacg tccagcattc aagaaggaag gagtagcagg ttggtagcgt tggtggatga
    12721 tttcattggc aatatcgatt gcaacagctt cattcccatc agcgaaataa agggcgttga
    12781 agaagacacg gtcttccata ttttcaagat agtattcacc gtcattagtc tttaaggcat
    12841 attgattgta aaatttataa gctgccatga atgacttgaa ttggaagttt tggtctttga
    12901 taaattgagc caattcttcc aagaactctg gacggtattt cttgataaag gctgtttcga
    12961 tgtagttgtg ttcaatgagg taattgattt tgtctttgat tgaatcaaaa accatagtgt
    13021 ttggaactac attttcttta aagaaagcat ccaaggcttc cttatcttta tgaagcatga
    13081 tttgtccatt aacaggacgg ttaatttcgt tattaagacg gaagtaagtc acgtcttcaa
    13141 gatgttttaa tcccataaaa tttcccttat ctaattacaa aagaaaggct tctaagttag
    13201 ccctaaaagc agtttcttct ggatgatgta ctaagattat gctaattgtt tcagttttcc
    13261 tggttggaaa cctgaaaaga cttcagttgg tgtttggata acaggagctg cgctaaaacc
    13321 gagctcttta acttgatcga cgtactcagg ttgctcatca agattgattt cacgataaga
    13381 gacattatta ctgtccaaga aacgcttggt cattttacat tggacacaat tgtttttaga
    13441 ataaacggtt accattgtgt aactcctctt caaaatttaa tactatctta gtatatcaga
    13501 aaataaaatt ttgtcaatga tttaaactaa atatagtggt gttcttttgt tgtttttata
    13561 tataaacaca atatatagtg tttgtattgt gaaatagcaa tgattatcct ataaattgtt
    13621 tttgaagaaa ctatatcctg tgttttgtta tctaggatag aagattttca gcaagttatc
    13681 tgaaaggaaa ttgaaggaaa atccagaaaa tgcttgaaaa aaatcctaga agatggtata
    13741 atactaaatt gtaagggtta tcacatataa ctcaaaaaaa gaaagaacaa aaggagagtc
    13801 aaactatggc ttctaaagat ttccacgtag tggcagaaac aggtattcac gcacgtccag
    13861 caacattgtt ggtacaaact gctagcaaat ttgcttcaga tatcactctt gagtacaaag
    13921 gtaaatcagt taaccttaaa tcaattatgg gtgttatgag tcttggtgtt ggtcaaggtg
    13981 ctgacgtaac tatctcagct gaaggtgcag atgcagatga cgctatcgct gcaatctcag
    14041 aaacaatgga aaaagaagga ttggcataag ggaaatgaca gaaatgctta aaggaatcgc
    14101 agcatctgac ggtgttgcag ttgcaaaagc atatctactc gttcagccgg atttgtcatt
    14161 tgagactatt acagtcgaag atacaaacgc agaagaagct cgccttgatg ccgctctaca
    14221 ggcatcacaa gacgagcttt ctgttattcg cgagaaagca gtaggtacgc tcggtgaaga
    14281 agcagctcaa gtttttgatg ctcacttaat ggttcttgct gacccagaaa tgatcagcca
    14341 aatcaaggaa actatccgtg cgaagaaagt gaatgcagaa gcaggtctga aagaagttac
    14401 agatatgttt atcactatct ttgaaggcat ggaagacaac ccatacatgc aagaacgcgc
    14461 agcggatatc cgcgacgtga caaaacgtgt attggcaaac cttcttggta aaaaattgcc
    14521 aaacccagct tctatcaatg aagaagtgat tgtgattgcg catgacttga ctccttcaga
    14581 tacagctcaa ttggacaaaa actttgtaaa agcttttgta accaacattg gtggacgtac
    14641 aagccactca gctatcatgg cacgtacact tgaaattgct gctgtattag gtacaaacaa
    14701 catcactgaa atcgttaaag acggtgacat ccttgctgtt aacgggatca ctggagaagt
    14761 gattatcaac ccaacagatg aacaagcggc agaatttaaa gcagctggtg aagcctatgc
    14821 gaaacaaaaa gctgaatggg cacttttgaa agatgctcaa acagtgactg ctgacggtaa
    14881 acacttcgag ttggctgcca atattggtac tccaaaagac gttgaaggtg ttaacaacaa
    14941 cggtgcagaa gctgttggac tttaccgtac agagttcttg tacatggatt ctcaagactt
    15001 cccaactgaa gatgagcagt atgaagcata caaggctgtt cttgaaggaa tgaacggtaa
    15061 acctgttgtc gttcgtacaa tggatatcgg tggagataag gaacttcctt acttcgatat
    15121 gcctcacgaa atgaacccat tccttggatt ccgtgctctt cgtatctcta tctctgagac
    15181 tggagatgct atgttccgca cacaaatccg tgctcttctt cgtgcgtctg ttcacggtca
    15241 attgcgtatc atgttcccaa tggttgcgct cttgaaagaa ttccgtgcag cgaaagcagt
    15301 ctttgatgaa gaaaaagcaa accttcttgc tgaaggtgtt gcagttgcgg ataacattca
    15361 agttggtatc atgatcgaga ttcctgcagc ggctatgctt gcagaccaat ttgctaaaga
    15421 agttgacttc ttctcaattg gtacaaacga cttgatccaa tatacaatgg cagcagaccg
    15481 tatgaacgaa caagtttcat acctttacca accatacaac ccatcaatcc tacgcttgat
    15541 taacaatgtg atcaaagcag ctcacgctga aggtaaatgg gctggtatgt gtggtgagat
    15601 ggctggtgac caacaagctg ttccacttct tgtcggaatg ggcttggatg agttctctat
    15661 gtcagcaaca tctgtacttc gtacacgcag cttgatgaag aaactcgaca cagctaagat
    15721 ggaagagtac gcaaaccgtg cccttacaga atgctcaaca atggaagaag ttcttgaact
    15781 tcaaaaagaa tacgttaatt ttgattaatc gaaaagtccc tgcaactcag ttacagggac
    15841 tttttttgat attttaaaaa gaattttcaa gaaaatcttt cttataaaaa gtccaacctt
    15901 gaaaaagtag tggtcagaac aaaaaatact taaatggttc ataaaattct tgacaagttg
    15961 gagatttagg agtaaactat taaccagtta agtaatagag aggagtttct gcaatttaga
    16021 aatgaattgc aactagaaat atcaaataga aagagagttt cgatgaaaat taataagaaa
    16081 taccttgttg gttctgcggc agctttgatt ttaagtgttt gttcttacga gttgggactg
    16141 tatcaagcta gaacggttaa ggaaaataat cgtgtttcct atatagatgg aaaacaagcg
    16201 acgcaaaaaa cggagaattt gactcctgat gaggttagca agcgtgaagg aatcaatgct
    16261 gagcaaatcg tcatcaagat aacagaccaa ggctatgtca cttcacatgg cgaccactat
    16321 cattattaca atggtaaggt tccttatgat gcgattttca gtgaagaatt actcatgaaa
    16381 gatccaaact ataagctaaa agatgaggat attgttaatg aggtcaaggg tggatatgtt
    16441 atcaaggtag atggaaaata ctatgtttac cttaaggatg ctgcccacgc ggataacgtc
    16501 cgtacaaaag aggaaatcaa tcgacaaaaa caagaacata gtcaacatcg tgaaggtggg
    16561 actccaagaa acgatggtgc tgttgccttg gcacgttcgc aaggacgcta tactacagat
    16621 gatggttata tctttaatgc ttctgatatc atagaggata ctggtgatgc ttatatcgtt
    16681 cctcatggag atcattacca ttacattcct aagaatgagt tatcagctag cgagttggct
    16741 gctgcagaag ccttcctatc tggtcgggga aatctgtcaa attcaagaac ctatcgccga
    16801 caaaatagcg ataacacttc aagaacaaac tgggtacctt ctgtaagcaa tccaggaact
    16861 acaaatacta acacaagcaa caacagcaac actaacagtc aagcaagtca aagtaatgac
    16921 attgatagtc tcttgaaaca gctctacaaa ctgcctttga gtcaacgaca tgtagaatct
    16981 gatggccttg ttttcgaccc agcgcaaatc acaagtcgaa cagctagagg tgttgcagtg
    17041 ccacacggag atcattacca cttcatccct tactctcaaa tgtctgaatt ggaagaacga
    17101 atcgctcgta ttattcccct tcgttatcgt tcaaaccatt gggtaccaga ttcaaggcca
    17161 gaacaaccaa gtccacaacc gactccggaa cctagtccag gcccgcaacc tgcaccaaat
    17221 cttaaaatag actcaaattc ttctttggtt agtcagctgg tacgaaaagt tggggaagga
    17281 tatgtattcg aagaaaaggg catctctcgt tatgtctttg cgaaagattt accatctgaa
    17341 actgttaaaa atcttgaaag taagttatca aaacaagaga gtgtttcaca cactttaact
    17401 gctaaaaaag aaaatgttgc tcctcgtgac caagaatttt atgataaagc atataatctg
    17461 ttaactgagg ctcataaagc cttgtttgaa aataagggtc gtaattctga tttccaagcc
    17521 ttagacaaat tattagaacg cttgaatgat gaatcgacta ataaagaaaa attggtagat
    17581 gatttattgg cattcctagc accaattacc catccagagc gacttggcaa gccaaattct
    17641 caaattgagt atactgaaga cgaagttcgt attgctcaat tagctgataa gtatacaacg
    17701 tcagatggtt acatttttga tgaacatgat ataatcagtg atgaaggaga tgcatatgta
    17761 acgcctcata tgggccatag tcactggatt ggaaaagata gcctttctga taaggaaaaa
    17821 gttgcagctc aagcctatac taaagaaaaa ggtatcctac ctccatctcc agacgcagat
    17881 gttaaagcaa atccaactgg agatagtgca gcagctattt acaatcgtgt gaaaggggaa
    17941 aaacgaattc cactcgttcg acttccatat atggttgagc atacagttga ggttaaaaac
    18001 ggtaatttga ttattcctca taaggatcat taccataata ttaaatttgc ttggtttgat
    18061 gatcacacat acaaagctcc aaatggctat accttggaag atttgtttgc gacgattaag
    18121 tactacgtag aacaccctga cgaacgtcca cattctaatg atggatgggg caatgccagt
    18181 gagcatgtgt taggcaagaa agaccacagt gaagatccaa ataagaactt caaagcggat
    18241 gaagagccag tagaggaaac acctgctgag ccagaagtcc ctcaagtaga gactgaaaaa
    18301 gtagaagccc aactcaaaga agcagaagtt ttgcttgcga aagtaacgga ttctagtctg
    18361 aaagccaatg caacagaaac tctagctggt ttacgaaata atttgactct tcaaattatg
    18421 gataacaata gtatcatggc agaagcagaa aaattacttg cgttgttaaa aggaagtaat
    18481 ccttcatctg taagtaagga aaaaataaac taatgaaaaa tgaaagtctc gataaagagg
    18541 ctttcatttt tattatgtat atatgtaaaa ttcttgacaa gcaatattaa aaagagtaaa
    18601 ctattaacta gttaattaac cggtttatta ctttatagtg aatcaaatat acttaagaaa
    18661 agaggaaaga atgaaaat
//
LOCUS       DAVKWZ010000037        18612 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000037 DAVKWZ010000000
VERSION     DAVKWZ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18612
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.037"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(31..276)
                     /locus_tag="VW854_001389"
     CDS             complement(31..276)
                     /locus_tag="VW854_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEV0262613.1"
                     /translation="MEQSPQKKKGTTLEHIILLRGVTPNGKNAIPKMSYLVDILTEAD
                     FQQVRTYIQSGNIILESNLALEEIREQVHTLLDFLQN"
     gene            complement(291..1463)
                     /locus_tag="VW854_001390"
     CDS             complement(291..1463)
                     /locus_tag="VW854_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV0262614.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGANPI"
     gene            complement(1522..2367)
                     /locus_tag="VW854_001391"
     CDS             complement(1522..2367)
                     /locus_tag="VW854_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV0262615.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            2492..3049
                     /locus_tag="VW854_001392"
     CDS             2492..3049
                     /locus_tag="VW854_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV0262616.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            3068..3535
                     /locus_tag="VW854_001393"
     CDS             3068..3535
                     /locus_tag="VW854_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEV0262617.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            3624..4253
                     /gene="upp"
                     /locus_tag="VW854_001394"
     CDS             3624..4253
                     /gene="upp"
                     /locus_tag="VW854_001394"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEV0262618.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            4427..5017
                     /gene="clpP"
                     /locus_tag="VW854_001395"
     CDS             4427..5017
                     /gene="clpP"
                     /locus_tag="VW854_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEV0262619.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            5096..5344
                     /locus_tag="VW854_001396"
     CDS             5096..5344
                     /locus_tag="VW854_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEV0262620.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            5445..6605
                     /locus_tag="VW854_001397"
     CDS             5445..6605
                     /locus_tag="VW854_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV0262621.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKNNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            6873..7742
                     /locus_tag="VW854_001398"
     CDS             6873..7742
                     /locus_tag="VW854_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV0262622.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLVTAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            7746..8702
                     /locus_tag="VW854_001399"
     CDS             7746..8702
                     /locus_tag="VW854_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV0262623.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKSPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGATVSAIVLGILNMLLQDVTS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            8702..9466
                     /locus_tag="VW854_001400"
     CDS             8702..9466
                     /locus_tag="VW854_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0262624.1"
                     /translation="MTLLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLAGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            9466..10176
                     /locus_tag="VW854_001401"
     CDS             9466..10176
                     /locus_tag="VW854_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0262625.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            10484..11140
                     /locus_tag="VW854_001402"
     CDS             10484..11140
                     /locus_tag="VW854_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV0262626.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKIKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKIGILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDESIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            11248..12343
                     /gene="prfB"
                     /locus_tag="VW854_001403"
     CDS             join(11248..11319,11321..12343)
                     /gene="prfB"
                     /locus_tag="VW854_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEV0262627.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            12361..13053
                     /gene="ftsE"
                     /locus_tag="VW854_001404"
     CDS             12361..13053
                     /gene="ftsE"
                     /locus_tag="VW854_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEV0262628.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKKRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            13046..13972
                     /locus_tag="VW854_001405"
     CDS             13046..13972
                     /locus_tag="VW854_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0262629.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
     gene            14258..16438
                     /locus_tag="VW854_001406"
     CDS             14258..16438
                     /locus_tag="VW854_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV0262630.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFAPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVALEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVTAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            16490..17305
                     /locus_tag="VW854_001407"
     CDS             16490..17305
                     /locus_tag="VW854_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV0262631.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAVLNWK"
     gene            17511..>18612
                     /locus_tag="VW854_001408"
     CDS             17511..>18612
                     /locus_tag="VW854_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000465984.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV0262632.1"
                     /translation="MFFQDRKIKYSIRKLSVATVSLAIGFLFAPAALGVAQVAQADEL
                     STTEQVENKAENQVSVVSFATEQEESETTYVDTTNKVTWTFAGYDKEKIVVGKGRQTF
                     LGSWVPTPNPEYVFKSSKAGVPLPQSILGMLPKDEASYKVGDTIVAKQPAVESVVEEE
                     KDYVWTFKGYDQKNATYNGKRVTFTGIWEVTPRPHHVSYTFVSVTSGVDLPKFIQKKA
                     PKDGSTYFNRTRVFAEEPTTKTYIDSVNDGTWTFKGYDAASAVVNKANVEFVGKWEFK
                     ANPTNAETYTPQVTEETIKVGQTPDLTDNVTNLPNLPAGTKVVDITPAGQIDTTKPGT
                     YTGKVRVDYPDGSSTEVSVSVNVLPAPETQTYK"
BASE COUNT         5652 a         3408 c         4044 g         5508 t
ORIGIN      
        1 tgctggtatt atcaatcatg aactaggatt ctagttttgc aggaagtcta atagagtatg
       61 aacctgttct cgtatttctt cgagagctaa gttactttca agaataatat ttccactttg
      121 aatataagtt cgaacttgtt gaaaatcagc ttctgtcaag atatctacta gataagacat
      181 tttagggata gcattttttc cattaggagt gactcccctt agcaagataa tatgttccaa
      241 agtagttcct ttcttttttt ggggggattg ttccatttag taatgtatat ttagattgga
      301 tttgctcccg ccacccagcc ggtacagagg gcagaagtga tgttaaagcc acccgtgtgg
      361 gcattgatat ccataacttc gcctgcaaag tggaggccag gtaccagctt actttcaagg
      421 gttttaggat tgatttcctt gagactgact ccacccttgg taacaaagga ctttgcaagg
      481 gacatttttc cagttacagg aattttaagt tctttaatgg actggacaag ttgttctcgt
      541 tccttttcag tcagttgttt gactttttca ggatatcctt gtacaaaaaa ttcggccaag
      601 cgttctggta acaaggtttt taaagcgttt ttcaaggatt tttcccgatt ttcttctaga
      661 aatgtaacca agtccttctc agaaagttga ggcaaaacat cgagtgagag aacctcccca
      721 cctttgacaa agctagacat gcgtagggca gcaggacctg acaaaccaaa gtgggtaaag
      781 agtaaatcat gagtgatgac atgcttacca taacttaggg tcacatcgtc cagagaaatc
      841 ccttgtaagg ctttatgtgg aaaatctgtt aataaaggac tttcagcagc ctcaagatcg
      901 gtgatggtat gcttaaaatg gcgagcaatc tcgtgaccaa aaccagtcga accagtcgaa
      961 ggataagact taccacctgt tgtgacaatg agtttctcac aagtgaaggt ttgatccgct
     1021 gacttaagga caaactggtc atctactttt ttaacagaaa cgatttctat ttgagtagca
     1081 acttgaccac ctagttcggt gattttcttt tccaaagctt cgataatagt ccgagacttg
     1141 tcactggctg gaaagacgcg tccgtggtct tcgaccttaa gtttaacacc attttctgta
     1201 aaaaagttga tgatgtcatg attatcgaac tgggagaaaa cactgtaaag aaagcgtccg
     1261 tttccaggaa ttccagctag caggttgtct aagctaccat tgttggtcac attgcaacgt
     1321 cccccaccag tcccagctaa tttttttcca agtttccgat ttttttcgat gaggagggtt
     1381 ttctgtccat aaaagctact ggaaatcgta gccatcatac cagcaggtcc cccaccgatg
     1441 acaatagtat caaaatgttt catagctcta ttgtaccaca aaaaaacaag agatgatggt
     1501 cacctcttgt caagaatgca attaatcaat ttcatagccc atcagcaaac cgccctcttc
     1561 tgcatagaaa ctgcagagac cagaggttgg tagaatttta atatccgctt gtgggaaggt
     1621 ttcacggatt cgctctgaga gctgttgaca acatttttcg ttattgcgtt gggccatgac
     1681 aatacggcca ccagcatatc cagcttttac taactcatca taggcagctt gaactgattt
     1741 ctttgatccc cttgcttttt gtagcaattc gagagttcca gtttcactag cttttccgac
     1801 catacgaatg ttgagaaggc caactaccgt accgataagc ttactcaaac ggccgttctt
     1861 caccaagtta tcgactttgg ctaggacaaa gagcaactta gttttttctt gataggcggt
     1921 gatagcttca accacttctt caaaagacaa gccttggtca atcaagtcat tcaatttttc
     1981 tacgagtagg tcaacttcac caccagcaga taaactatca atcacatgaa tcttagtgtc
     2041 aggatggtct tccagataaa tattctttgc tagttgagcg ctattgtgac tgccagaaag
     2101 ggtacctgtg atggttacta ggaaaatgtt tttggcacct tcaaatgctc gcaaatagtc
     2161 atctgggctt ggacaagctg attttgaagc ttctgcagtt gcatacatgg tttccatcat
     2221 ttggtcaata tcgagactgg cgtcatcaac aaagacctga tcagctactt gaatggttaa
     2281 ggggacactt acaaaggttg tgttaatagc tggtgttggc agttgacgat aatcacaacc
     2341 agagtcagca ataatcttcc aagtcataga aattctccat ctttgtcagt tatacattga
     2401 caaaggttct gtcttttttt acaattatat catgaaaact cttgaaacaa aagcatcatc
     2461 cgtctgttgt gacaagtaga aagaaaatgt tatgtctgaa cgtagaatct ctgaaaagtc
     2521 tcttgaaaat ctcagaaaat caaaccaaga atccaattta ttaaccagag aagccattga
     2581 aacagccctc ttgcaactct tggaaaaaaa ggagctgacc aagattagta tttctgaatt
     2641 ggtcaaacgt gcaggtgttt cgcgtgcggc cttttatcgc aattatgact ccaaagagga
     2701 aattttagag agcgtcttta aacgaactgt ccacaatatt atggaacagc tgcatcatta
     2761 cgatttaaag acagaccttt atttggtttg ggttcacctt ttccgggagg ccagaaagga
     2821 agctagagta attcaattgg ccttggatta ccatctggaa aaaatctttg tccaagccat
     2881 gcaggaattt ctagaaaaat accatgggaa atcaaaaggt gtcagctctt atcttcattc
     2941 cttctggagc tcagccatcg tctctgtcct tctaaaatgg atcaaggatg gcatgaaggt
     3001 accagctgaa aagattgcag atttaggttt accatttttt aaaaaataga gaaaaaggag
     3061 aaaagagatg actgaaaaaa gactggcctg ggatgagtat tttgcagccc aagccctact
     3121 aattgcgaat cgttccactt gtaaacgtgc taaagtgggc gcgattctgg taaaagataa
     3181 taaggttatt tccactggtt acaatggttc ggtgtcaggg actgagcatt gtattgatca
     3241 cgaatgtttg gtcattgaag gccactgtgt tcgcaccctt cacgctgagg tcaatgctat
     3301 ccttcaaggt gctgagcgtg gtgttcccaa aggctttaca gcctatgtaa cccattttcc
     3361 ttgtctcaac tgtacaaaac aattgcttca ggtcggctgc aagcgcgtgg tttatatcaa
     3421 ccagtaccga atggacgact acgcccaata cctttatcaa gaaaaaggga cagaattgac
     3481 ccatttacca cttgagacag tacagacagc tcttaaagag gctgatctaa tgtaaaaatt
     3541 atcaaaaata aatggcttag aaagattttt aaaccgtttt ttggtataat aagaagaata
     3601 aattgaaaga aggaattcca aaaatgggaa aaattgaagt tattaatcac ccactgattc
     3661 aacacaaatt gtcaatcttg cgtcgtacag atacttctac aaaagctttt cgtgagctag
     3721 tagatgagat tgcaatgttg atggggtatg aagtacttcg tgatcttcca ctagaagatg
     3781 tggaaatcga aacaccaatt acaaaaacag ttcaaaaaca attggcaggt aagaaattgg
     3841 ccatcgtccc aatcttgcgt gcaggtatcg ggatggttga tggtctcttg agcttggttc
     3901 cagctgctaa agttggccac atcggtatgt accgtgatga agaaacactt caaccagttg
     3961 aatacttggt gaaattgcct gaggacattg accaacgtca aatttttgta gtagacccaa
     4021 tgttggcaac aggtggctca gcaatcttgg ctgttgattc tcttaaaaaa cgtggcgcat
     4081 caaatatcaa atttgtctgc cttgtatctg ctccagaggg tgtaaaagcc cttcaagaag
     4141 ctcatccaga tgtagaaatc tttacagcag ccttggatga acgtttgaac gaacacggtt
     4201 atatcgttcc aggtcttgga gatgctggag accgcttgtt cggtacaaaa taagatcgaa
     4261 aagaagaatg acttggaaaa gtatttctag tcacgaaagg aggttgggtt tttgtttctg
     4321 tctaatgaaa gcagagcaaa aatttgacct tttttgacca agatattata atagtcttat
     4381 cttcagtcat ttgaccaaca aaattaaaac tcaaaaggag aaatgaatga ttcctgtagt
     4441 tattgaacaa acaagccgtg gagaacgttc ttacgatatt tactcacgtc ttctcaaaga
     4501 ccgcatcatt atgctgacag gtccggttga agacaatatg gctaactctg ttattgccca
     4561 attgcttttc ttggatgccc aagatagtac gaaagatatt tacctttatg tcaatacacc
     4621 aggtggttct gtttcagctg gtttggcaat cgtagatacc atgaacttta tcaaggcaga
     4681 tgtccaaacc attgttatgg gaatggctgc atctatgggg actgtcatcg catcaagtgg
     4741 ggcaaaaggc aaacgtttca tgcttccaaa tgctgaatac atgattcacc aaccaatggg
     4801 cggtacaggt ggtggtaccc aacaaactga tatggctatc gctgcagaac acttgctcaa
     4861 aactcgtaat accttggaaa aaatcttggc tgaaaattca ggtcagtcaa tggaaaaagt
     4921 ccatgcagat gcagaacgtg ataactggat gagcgcccag gaaacacttg aatatggctt
     4981 tattgatgaa attatggcca acaattcatt gaactaatga tgatagaagg caaactcgac
     5041 tgggtttgct ttttttggta taatagggag agatttctta gaaagaggat ttatcatgtt
     5101 tgaaaaagtc aatcgctctg gcttgattat ctatctttac tataatcgtg atgccaaaaa
     5161 actgcaggat tatggagata ttacctatca ttccaagaaa catcgttact tacaactcta
     5221 tgttccaact caagaagtgg agcaattggt cggacgcttg agcaaggaaa aatttattaa
     5281 aaaagttagg gtttgtcata tccaagagtt ggaaacaccc tttgtgggca atctttatcg
     5341 ataggaaaac gttatcatcg aaaaagttca aggaaaacat tgacaatttt ctgataattc
     5401 ggtatattct taacagacta tttaagaaat aaggagacaa aaagatgaag aaaaaatttg
     5461 ccctatcgtt tgtggcgctt gcaagtgtag cacttcttgc agcctgtgga gaagtgaagt
     5521 ctggagcagt caacactgct ggtaactcag tagaggaaaa gacaattaaa atcgggttta
     5581 actttgaaga atcaggttct ttagctgcat acggaacagc tgaacaaaaa ggtgcccaat
     5641 tggctgttga tgaaatcaat gccgcaggtg gtatcgatgg aaaacaaatc gaagtagtcg
     5701 ataaagataa taagtctgaa acagctgagg ctgcttcagt tacaactaac cttgtaaccc
     5761 aatctaaagt atcagcagtc gtaggacctg cgacatctgg tgcgactgca gctgcggtag
     5821 cgaacgctac aaaagcaggt gttccattga tctcaccaag tgcgactcaa gatggattga
     5881 ctaaaggtca agattacctc tttattggaa ctttccaaga tagcttccaa ggaaaaatta
     5941 tctcaaacta tgtttctgaa aaattaaatg ctaagaaagt tgttctttac actgacaatg
     6001 ccagtgacta tgctaaaggg attgccaaat ctttccgcga gtcatacaag ggtgaaatcg
     6061 ttgcagatga aactttcgta gcaggtgaca cagacttcca agcagccctt acaaaaatga
     6121 aagggaaaga ctttgatgct atcgttgttc ctggttacta taatgaggct ggtaagattg
     6181 taaaccaagc gcgtggtatg ggaattgaca aaccaatcgt tggtggtgat ggattcaacg
     6241 gtgaggagtt tgtacaacaa gcaactgctg aaaaagcatc aaacatctac tttatctcag
     6301 gcttctcaac tactgtagaa gtttcagcta aagctaaagc cttccttgac gcttaccgtg
     6361 ctaagtacaa tgaagagcct tcaacatttg cagccttggc ttatgattca gttcaccttg
     6421 tagcaaacgc agcaaaaggt gctaaaaatt caggtgaaat caagaataac cttgctaaaa
     6481 caaaagattt tgaaggtgta actggtcaaa caagcttcga tgcagaccac aacacagtca
     6541 aaactgctta catgatgacc atgaacaatg gtaaagttga agcagcagaa gttgtaaaac
     6601 cataatagaa aaatgttgaa atagggaatg agcctttgac tcactccctg tttcgatatt
     6661 taatactctt cgaaaatctc ttcaaactgc gtcaacgtcg ccttggatta tatatgtgac
     6721 tgacttcgtc agtcttatct acaacctcaa agcagtgctt tgagcaacct gcggctagtt
     6781 tcctagtttg ctctttgatt ttcattgagt ataagaacct atcaaaaagt gagggaaaac
     6841 cctcggaatt ataaatagaa agagtgaatc ttatgctcca acaactcgta aatggtttga
     6901 ttctaggtag tgtttacgcg ctgttagccc taggatatac catggtttac ggaattatca
     6961 agctcatcaa cttcgcccat ggtgatattt atatgatggg agcctttatc ggttatttct
     7021 tgatcaattc tttccaaatg aatttctttg tagcgcttat tgtagctatg ctagtgacag
     7081 ctattcttgg tgtcgtgatt gagtttcttg cttaccgacc tttgcgccac tctactcgta
     7141 ttgctgtttt gattacggct attggggttt ctttcctatt ggagtatgga atggtctatc
     7201 tggttggtgc caatacccgt gccttccctc aagcgattca aacagttcga tatgatttgg
     7261 gaccaattag cttaacaaat gtgcagttaa tgattttggc catttccttg attttgatga
     7321 ttttgttaca agtcattgtc caaaagacta agatggggaa agccatgcgt gcagtatcag
     7381 tagatagcga cgcggcacaa ttgatgggga tcaatgtaaa ccgtacgatt agctttacct
     7441 tcgctttggg ttctgctctt gcgggtgcgg ctggtgttct gattgctctt tattataact
     7501 ctcttgagcc tttgatgggg gttactccag gtcttaaatc tttcgttgcc gcagtacttg
     7561 gtggtatcgg aattattcct ggtgcggctc ttggtggctt tgtgattggt ctattggaaa
     7621 cctttgcgac agcctttggg atgtcagatt tccgtgatgc cattgtttat ggaatcttgt
     7681 tgttgatctt gattgtccgc ccagctggta tccttggtaa gaatgtgaaa gagaaggtgt
     7741 aaacgatgaa ggaaaattta aaagttaata ttctatggtt actccttttg ttagctggct
     7801 atagcttgat tagtgtactg gtttcagtcg gagtacttaa tctattctat gtacagattt
     7861 tacaacaaat tggaattaat attattttgg ctgttggtct caacttaatc gttggttttt
     7921 caggacaatt ttcacttggt catgctggtt tcatggcgat tggtgcctat gcagcagcta
     7981 ttattggttc taaatcacca acctacggtg ccttctttgg agctatgctt gtaggggctt
     8041 tgctttcagg agcagttgcc ttacttgtcg gcattccaac cttgcgcttg aagggggact
     8101 atcttgcggt agcaactcta ggtgtttctg aaattatccg tatctttatc atcaatggtg
     8161 gaagccttac aaatggtgcg gcaggtatct tagggattcc taactttaca acttggcaaa
     8221 tggtttactt ctttgtcgtg attacaacca ttgcaacctt gaacttcttg cgtagcccaa
     8281 ttggtcgttc aaccctctct gttcgtgaag atgaaatcgc tgctgagtca gttggggtta
     8341 atacgactaa aattaaaatc atcgcttttg tctttggtgc cattactgca agtattgctg
     8401 ggtcacttca ggcaggattt atcgggtctg ttgtaccgaa agattacacc ttcatcaact
     8461 caatcaacgt tttgattatt gttgtatttg gtggactcgg ttccattaca ggtgcgactg
     8521 tttcggctat tgttctggga attttgaata tgcttctcca agatgttact agtgtgcgta
     8581 tgattattta cgctttggcc ttggtattgg taatgatttt cagaccaggt ggactccttg
     8641 gaacatggga actgagccta tcacgtttct ttaaaaaatc taagaaggag gaacaaaact
     8701 aatgacatta cttgaagtaa aacagttaac caaacatttt ggtggtctaa cagctgttgg
     8761 agatgtgact cttgaattga acgaagggga actggttgga ttaatcggtc caaacggagc
     8821 tgggaaaacc acccttttca accttttggc cggtgtttat gaaccaagcg agggaacagt
     8881 aactctagat ggtcaccttt tgaatgggaa atcaccttat aagattgcct ctttgggact
     8941 tggacgtact ttccaaaata tccgtctctt taaagattta acagttttag acaatgtttt
     9001 gattgctttt ggaaaccatc acaaacaaca tgtttttact agtttcttac gcttaccagc
     9061 tttttacaag agtgaaaaag aattaaaggc taaagctttg gaattgttga aaatctttga
     9121 tttagatggt gatgcagaga ctcttgctaa aaatctttcc tacggacaac aacgtcgttt
     9181 ggaaattgtt cgtgcccttg ctacggaacc taaaattctc ttcttagatg aaccagcagc
     9241 aggtatgaac ccacaggaaa cagccgaatt gactgagtta attcgtcgta tcaaagatga
     9301 atttaagatt acaatcatgt tgattgaaca cgatatgaat ctggtcatgg aagtaacaga
     9361 acgtatctac gtacttgaat atggccgttt aattgctcaa ggaactccag acgaaattaa
     9421 gaccaataaa cgcgttatcg aagcttatct aggaggtgaa gcctaatgtc tatgttaaaa
     9481 gttgaaaatc tttctgtgca ttacggtatg atccaagcag tccgtgatgt aagctttgaa
     9541 gttaatgaag gagaagttgt ttcccttatc ggtgccaacg gtgcaggtaa gacaactatt
     9601 cttcgcacct tgtcaggttt ggttcgacca agttcaggaa agattgaatt tttaggtcaa
     9661 gaaatccaaa aaatgccagc tcagaaaatt gtggcaggtg gtctttcaca agttccagaa
     9721 ggacgccacg tctttcctgg cttgactgtt atggaaaatc ttgaaatggg agctttctta
     9781 aagaaaaatc gtgaagaaaa tcaagctaac ttgaagaagg ttttctcacg ctttcctcgt
     9841 cttgaagaac gtaagaacca agatgcagct actctttcag gaggggaaca acaaatgctt
     9901 gccatgggac gcgctcttat gtcaacacca aaacttcttc ttttagatga accatcaatg
     9961 ggacttgccc caatcttcat ccaagagatt tttgatatca ttcaagatat tcagaagcaa
    10021 ggaacaaccg tcctcttgat tgaacaaaat gccaataaag cacttgcaat ctctgaccga
    10081 ggatatgtac tggaaacagg gaaaatcgtc ctatcaggaa caggaaaaga actcgcctca
    10141 tcagaagaag tcagaaaagc atatctaggt ggctaaaaca atccagtgga ttgttttagt
    10201 cggcagatga agattacgaa gtaatcatca atatagtccg ggggaccttt ttagtcggtg
    10261 gatagagatt gcaaacaaat atgcatctac attgaaagct taatttctaa taattgaaaa
    10321 aatcgaatga aaaatttctt accttcattc acagagctct atttcagagc tctttttgct
    10381 agcttattca tacttttttg aatttcgaaa aagaaatgta agcgtttgat agatttacaa
    10441 aaagattgta taatggggat aagaatagaa aaggagaagt ctcatggcag ttaaagattt
    10501 tatgacccgc aaggtagttt atattagtcc agatacaaca gtatctcatg cagcagattt
    10561 gatgagagag caaggtttgc accgtctgcc tgttatcgaa aatgatcaat tagttggttt
    10621 ggtgactgag ggaaccattg cacaagcaag tccatctaaa gcaacaagtc tttctatcta
    10681 tgagatgaat tatcttctga ataagataaa agtaaaagat gtcatgattc gcgatgttgt
    10741 cactgtctca ggctatgcta gtctagaaga tgcaacttat ctgatgttga aaaataagat
    10801 tggtattctc cctgtcgtag ataaccatca agtatacgga gttattactg accgtgatgt
    10861 tttccaagcc tttcttgaaa ttgcaggtta tggcgaagaa gggattcgtg tacgctttgt
    10921 tacagaagat gaagttggtg ttcttggaaa aattgtttct ttgattgtag aagaaaattt
    10981 gaatatctcc catacagtca atattccgcg taaggatggt aaggtgatta tcgaagtgca
    11041 aatcgatgaa tcaattgatt taccagcctt gaaagaaaaa tttgaagcaa atggtattca
    11101 agtggaagaa atcgctcgca cttcagcaaa agtcttgtaa gaagggaagt ccaaaggctt
    11161 cttttttcat gaaaagggga ttagagcaaa agatggaaag aaatgataaa atatgctata
    11221 atgaaataat gtaaaaaagg agtatttatg gacatttcag taattcgtca gaaaattgac
    11281 gcaaatcgtg aaaaattagc ttctttcagg gggtctcttt gacctcgaag ggctagagga
    11341 agagattgcc atcttggaaa acaagatgac agaacctgat ttttggaacg ataatattgc
    11401 ggcccaaaaa acgtcgcaag aattgaatga attaaaaaac acttacaata ccttccataa
    11461 gatggaagag ttgcaggatg aagtcgaaat tttattggat tttttggctg aagacgagtc
    11521 agtgcatgat gaactggtag cgcagttagc cgaacttgat aagataatga ccagctacga
    11581 gatgactcta ctcttgtcag aaccttatga ccacaacaat gccatcttgg aaatccatcc
    11641 aggttctggt ggtactgagg cgcaggactg gggtgatatg ttgcttcgta tgtatactcg
    11701 ttatggtaat gctaaaggct ttaaagtgga agtgttggat taccaagcag gtgatgaggc
    11761 tggtattaag tcggtaactt tatcatttga agggcctaat gcctatggtc tcctcaagtc
    11821 agaaatgggt gttcaccgct tagtgcgaat ctcaccattt gactctgcca aacgtcgcca
    11881 tacctctttc acatctgtag aagtgatgcc agaattggat gatactattg aagtggaaat
    11941 ccgtgaagat gatatcaaga tggatacctt ccgttcaggt ggtgccggtg gacaaaacgt
    12001 caataaggtt tcaacaggtg tacgtttaac ccacattcca actggaattg ttgtccaatc
    12061 aacagtggat cgtacccagt atggaaatag agatcgtgcc atgaagatgt tgcaggctaa
    12121 gctctatcaa atggagcaag agaagaaggc tgcggaggta gattctctca aaggtgagaa
    12181 aaaggagatt acttggggaa gtcaaatccg ttcttatgtc ttcacgcctt atactatggt
    12241 aaaagatcac cgaactagct ttgaggttgc tcaggtagat aaggttatgg atggggacct
    12301 agatggtttt atcgatgctt atctcaagtg gcgaattagc taagatagaa aggaactcac
    12361 atgtcaatta ttgaaatgag agatgtcgtt aaaaaatacg acaacggaac gactgctcta
    12421 cgcggtgttt cggtcagcgt tcaaccgggg gaatttgctt acatcgtagg accttcagga
    12481 gcagggaagt caacttttat tcgttctctg tatcgtgaag taaaaatcga taaaggaagc
    12541 ctatcagttg ctggttttaa tctggttaag atcaaaaaga aagatgtccc gcttctacgt
    12601 cgtagtgttg gggttgtctt tcaggattat aaattgttac caaagaaaac tgtctatgaa
    12661 aatattgctt acgctatgga agtaatcggg gaaaatcgcc gtaatatcaa aaaacgagtg
    12721 atggaagttt tggacttggt tggattgaag cataaggttc gttctttccc aaatgaactc
    12781 tcaggtgggg agcaacagcg gattgcgatt gcgcgtgcaa ttgtaaataa tcccaaagta
    12841 ttgatagctg atgagccaac aggaaatctg gatccggata attcatggga aattatgaat
    12901 ctcttggaac ggattaacct acaaggaaca actattttga tggcgactca taatagccag
    12961 attgtaaata ccttgcgcca ccgtgtcatt gccattgaaa atggccgtgt cgttcgtgac
    13021 gaatcaaaag gagagtatgg atacgatgat tagtagattt tttcgccatt tatttgaagc
    13081 cttaaaaagt ttgaaacgaa atggttggat gacagtagct gctgtcagtt cagtcatgat
    13141 tactttgacc ttggtggcaa tatttgcatc tgttattttc aatacagcga aactagctac
    13201 agatattgaa aataatgtcc gtgtagtagt ttatatccga aaggatgtgg aagataatag
    13261 tcagacaatt gaaaaagaag gtcaaactgt tacaaataat gactaccaca aggtatatga
    13321 ttctttgaag aacatgtcta cggttaaaag tgttaccttt tcaagtaaag aagaacaata
    13381 tgaaaaatta accgagataa tgggagataa ctggaaaatc tttgaaggag atgccaatcc
    13441 tctctatgat gcctatattg tagaggcaaa cactccaaat gatgtaaaaa ctatagccga
    13501 agatgctaaa aaaattgaag gtgtctctga ggttcaagat ggcggtgcca atacagaaag
    13561 actcttcaag ttagcttcat ttatccgtgt ttggggacta gggattgctg ctttgttaat
    13621 ttttatcgca gttttcttga tttcaaatac cattcgtatt accattattt cccgcagtcg
    13681 cgaaattcaa atcatgcgct tggtcggagc taaaaacagt tatatccgtg gaccgttctt
    13741 gttagaagga gcctttatcg gtttattggg agctatcgca ccatctgttt tggtctttat
    13801 tgtttatcaa attgtttacc aatctgtcaa caaatcgttg gtagggcaaa atctatccat
    13861 gattagtcca gatttattta gtccgttgat gattgcccta ctatttgtga ttggggtttt
    13921 cattggttca ttgggatcag gaatatccat gcgccgattc ttgaagattt aggtaaaata
    13981 gctgctttta tgaggagatt gtaaaatctc cttttttgct acaagagttt ttgaaaagag
    14041 atgcgcagaa gaaaagagct tccaaagaag tcccccagag aagacttctt gatggctaat
    14101 ttgaacttga aatttggcgc actagaaatt ttttataaaa aagtgtaatt tttttataaa
    14161 agcctttaca aaaaaaatta aagtggtata attaaatcat agaaagtgca aacgttttcg
    14221 taaaacgttt gcctaaataa aaataaagga gatttgaatg atgaaagata cattcaaaaa
    14281 tgtcttgtct ttcgaatttt ggcaaaaatt cggtaaggct ttgatggtag ttatcgcggt
    14341 tatgccggct gctggtttga tgatttcaat cggtaagtct atcgtgatga ttaacccaac
    14401 ctttgcacca cttgtcatca caggtggaat tcttgagcaa atcggttggg gggttatcgg
    14461 taaccttcac attttgtttg ccctagccat tggaggaagc tgggctaaag aacgtgctgg
    14521 tggtgctttc gccgctggtc ttgccttcat cttgattaac cgtatcactg gtacaatctt
    14581 tggtgtatca ggcgatatgt tgaaaaatcc agatgctatg gtaactactt tctttggtgg
    14641 ttcaatcaaa gttgctgatt actttatcag tgttcttgaa gctccagcct tgaacatggg
    14701 ggtattcgta gggattatct caggttttgt aggggcaact gcttacaaca aatactacaa
    14761 cttccgtaaa cttcctgatg cactttcatt cttcaacggg aaacgtttcg taccatttgt
    14821 agttattctt cgttcagcaa tcgctgcaat tctacttgct gctttctggc cagtagttca
    14881 aacaggtatc aataacttcg gtatctggat tgccaactca caagaaactg ctccaattct
    14941 tgcaccattc ttgtatggta ctttggaacg tttgctcttg ccatttggtc ttcaccacat
    15001 gttgactatc ccaatgaact acacagctct tggtggtact tatgacattt taactggtgc
    15061 agctaaaggt actcaagtat tcggtcaaga cccactatgg cttgcatggg taacagacct
    15121 tgtaaacctt aaaggtactg atgctagtca atatcaacac ttgttagata cagtacatcc
    15181 agctcgtttc aaagttggac aaatgatcgg ttcattcggt atcttgatgg gtgtgattgt
    15241 tgctatctac cgtaatgttg atgctgacaa gaaacataaa tacaaaggta tgatgattgc
    15301 aacagctctt gcaacattct tgacaggggt tactgaacca atcgaataca tgttcatgtt
    15361 catcgcaaca cctatgtatc ttgtttactc acttgttcaa ggtgctgcct tcgctatggc
    15421 tgacgtcgta aacctacgta tgcactcatt cggttcaatc gagttcttga ctcgtacacc
    15481 tattgcaatc agtgctggta ttggtatgga tatcgttaac ttcgtttggg taactgttct
    15541 ctttgctgta atcatgtact ttatcgcaaa cttcatgatt caaaaattca actacgcaac
    15601 tccagggcgc aacggaaact acgaaactgc tgaaggttca gaagaaacca gcagtgaagt
    15661 gaaagttgca gcaggctctc aagctgtaaa cattatcaac cttcttggtg gacgtgtaaa
    15721 catcgttgat gttgacgcat gtatgactcg tcttcgtgta actgttaaag atgcagataa
    15781 agtaggaaat gcagagcaat ggaaagcaga aggagctatg ggtcttgtca tgaaaggaca
    15841 aggggttcaa gctatctacg gtccaaaagc tgacattttg aaatctgata tccaagatat
    15901 ccttgattca ggtgaaatca ttcctgaaac tcttccaagc caaatgactg aagcacaaca
    15961 aaacactgtt cacttcaaag atcttactga ggaagtttac tcagtagcag acggtcaagt
    16021 tgttgctttg gaacaagtaa aggatccagt atttgctcaa aaaatgatgg gtgatggatt
    16081 tgcagtagaa cctgcaaatg gaaacattgt atctccagtt tcaggtactg tatcaagcat
    16141 cttcccaaca aaacatgctt ttggtattgt gacggaagca ggtcttgaag tattggttca
    16201 cattggtttg gacacagtaa gtcttgaagg taaaccattt acagttcatg ttgctgaagg
    16261 acaaaaagtt acagcaggag atctccttgt cacagctgac ttggatgcta tccgtgcagc
    16321 aggacgtgaa acttcaacag tagttgtctt cacaaatggt gatgcaatta aatcagttaa
    16381 attagaaaaa acaggttctc ttgcagctaa aacagcagtt gctaaagtag aattgtaata
    16441 tacttgaggt tggaagctgt attccaacct cttattttgg gagaaaagaa tgaaattttt
    16501 aacactcaat actcacagtt ggatggagaa agaagcagag gaaaaattcc agattttgct
    16561 tgaagatatt cttgaaaagg actatgattt gatttgtttc caagaaatca atcaggagat
    16621 gacctcgtca gaggtggagg ttaatgacct ttatcaagct ttgccagcag ctgagcctat
    16681 tcaccaagac cattatgtta gactcttggt tgaaaagttg ttagagcaag ggaaaaatta
    16741 ctactggacc tgggcttata accatatcgg ctatgaccgc taccatgaag gtgtggctat
    16801 cttgtctaaa acacctattg aagccagaga aattttggtt tcagatgtgg atgatccaac
    16861 agactatcat actcgccgtg ttgccctagc tgaaactgta gtcgatggca aggagctagc
    16921 agttgccagt gttcatctct cttggtggga taaaggtttc caagaagaat gggcacgatt
    16981 tgaggctgtc ttgaaaaaat tgaacaagcc acttttacta gctggagatt ttaacaaccc
    17041 agcaggtcag gaaggttacc aagctatttt agctagtcca ttaggcttac aagacgcatt
    17101 tgaagttgct caagagaaaa gtggtagcta tactgttccg cctgaaattg atggctggaa
    17161 agggaacact gaaccccttc gaatcgatta tgtctttact accaaagagt tagcggtgga
    17221 aaatttacat gtcgtatttg atggtaacaa gagtccacaa gtgagtgatc actatggctt
    17281 gaatgcggta ttaaattgga aataaaatca aaaaatgaaa gggagcgagt cctttcattt
    17341 ttttacaaaa atccctataa ttactatttt ggagaaggag ttttttgtat atttgtatat
    17401 tatgaggaaa tatccacaaa taagttttta aaactttacg gtcaaaaaaa aatcgctaat
    17461 attaactatt tatgatagaa tgaaaattat gaaataaaag gaggactgga atgttttttc
    17521 aggatagaaa gataaaatat tcaatccgca aattgtcagt tgctactgtg tcccttgcga
    17581 ttggattttt gtttgcgcct gcggcgcttg gagtcgctca agtagctcaa gctgatgagc
    17641 tttcaactac tgaacaagtg gagaataaag cagagaatca ggtctcggtg gtctcttttg
    17701 caacagagca agaagaaagt gaaacgactt atgttgatac gacgaataaa gtgacttgga
    17761 catttgcagg atacgataaa gaaaaaattg ttgtaggaaa aggcagacag actttccttg
    17821 gaagctgggt acctactcca aatccagaat atgtattcaa atcatctaaa gcaggtgttc
    17881 cattaccaca aagtattttg ggaatgcttc caaaggatga agcttcttat aaggttggtg
    17941 acaccattgt agccaaacaa cctgctgtag aatcagtagt cgaagaagaa aaagactatg
    18001 tctggacctt caaaggttat gatcagaaga atgcgacgta caatggcaag cgtgtgacat
    18061 ttacaggtat ctgggaagta acgcctagac cgcatcatgt aagctatact tttgtaagtg
    18121 ttacatctgg tgttgatttg cctaaattta tccagaagaa agcaccaaaa gatggctcaa
    18181 cttacttcaa taggacgaga gtttttgcag aagagccgac aacaaagacc tacatagata
    18241 gtgtgaacga tggcacatgg accttcaagg gttatgacgc agctagcgcc gttgttaaca
    18301 aggctaatgt agagtttgta ggtaagtggg aattcaaggc gaatccgaca aatgcggaaa
    18361 cttatacgcc tcaggtaacg gaagaaacta tcaaggttgg acaaacacct gacttgaccg
    18421 ataatgtgac gaacttgcca aaccttccag ctggcactaa ggtagttgat attacacctg
    18481 caggtcaaat tgacacaaca aaaccaggta cttacacagg taaggtgcga gttgactatc
    18541 cagatggttc ttctactgag gtgtctgtat cagttaatgt tttgcctgca ccagaaactc
    18601 aaacctataa gg
//
LOCUS       DAVKWZ010000038        18069 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000038 DAVKWZ010000000
VERSION     DAVKWZ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18069
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.038"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(115..1050)
                     /locus_tag="VW854_001409"
     CDS             complement(115..1050)
                     /locus_tag="VW854_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEV0262633.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLIQCDNGKEIWLNSGEISLNSWK"
     gene            complement(1047..1907)
                     /locus_tag="VW854_001410"
     CDS             complement(1047..1907)
                     /locus_tag="VW854_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV0262634.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFYYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            2015..4177
                     /locus_tag="VW854_001411"
     CDS             2015..4177
                     /locus_tag="VW854_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEV0262635.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMIRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            4269..5528
                     /locus_tag="VW854_001412"
     CDS             4269..5528
                     /locus_tag="VW854_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV0262636.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADDTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATDGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATAKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            5542..6408
                     /locus_tag="VW854_001413"
     CDS             5542..6408
                     /locus_tag="VW854_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV0262637.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSIVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            6422..7255
                     /locus_tag="VW854_001414"
     CDS             6422..7255
                     /locus_tag="VW854_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV0262638.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            7566..9008
                     /gene="gtfA"
                     /locus_tag="VW854_001415"
     CDS             7566..9008
                     /gene="gtfA"
                     /locus_tag="VW854_001415"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEV0262639.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFRDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGERYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKALFLNWDKFWPENRSTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVVEIDLQNQTYRVIENGVEVSF"
     gene            9146..10339
                     /locus_tag="VW854_001416"
     CDS             9146..10339
                     /locus_tag="VW854_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEV0262640.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMNSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            11024..13003
                     /locus_tag="VW854_001417"
     CDS             11024..13003
                     /locus_tag="VW854_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV0262641.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLSPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            13070..14566
                     /locus_tag="VW854_001418"
     CDS             13070..14566
                     /locus_tag="VW854_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0262642.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGTLTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPVTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            14566..15492
                     /locus_tag="VW854_001419"
     CDS             14566..15492
                     /locus_tag="VW854_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0262643.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            15501..16568
                     /locus_tag="VW854_001420"
     CDS             15501..16568
                     /locus_tag="VW854_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0262644.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKEWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPHHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            16579..17505
                     /locus_tag="VW854_001421"
     CDS             16579..17505
                     /locus_tag="VW854_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0262645.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQTELARYQKGLN"
     gene            complement(17580..18069)
                     /locus_tag="VW854_001422"
                     /pseudo
     CDS             complement(17580..18069)
                     /locus_tag="VW854_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5324 a         3203 c         3791 g         5751 t
ORIGIN      
        1 gggatttacc cactacaaat attatagaga caataaaact tcagatccgt aaaacttaat
       61 tgaattacta atctgaagtt atttttatat cgtttatatt ataattgtgt tattttactt
      121 ccaactattg agagaaattt cgccactatt tagccagatt tcttttccgt tatcacattg
      181 aattaaaagt tttccatttt ctgagatgtc tttagcaagt cccttgtagt ctttttgctc
      241 tagtgtgaaa gtgacttctt ttcctagaat gaatgactgt tttttgtata ggtataatag
      301 ctcttctgct ggtgtttcga agaaagcacg ccagatttct atgatcaatt cattccttgt
      361 tataggagct gtagctttaa ataagctggc agctttttct tttaattcct gagggaagtc
      421 tttaatagtg aaattgatac ctactccaat aatgatatct gtgactaagc cagtttctac
      481 agaggtcatt gcttcagtaa ggattcctcc aattttatga ttgtttagat agatatcatt
      541 gacccatttt atgtcgacat ctattaaagt taggttctta atagctttgt agacagctcc
      601 agctacaagt agtgtgtagg atggtaattt gtcatagggg agatttggtt taagatggag
      661 tgtcatataa ataccacctt gtggtgagta gaaggaacgt tgaaaacggc ctcggcctgc
      721 tgtttgatag gaagctagat agagggtatt tgcttcatgg cctaaatcaa ttgcttcttt
      781 tgcatctagt tgtgttgatt ttgtttcggg tttaaagctg actttaattg gaagattttc
      841 ttctagaatc tctggaagaa taaggtcacc attcatcagt ttatatcctc tatttttgat
      901 actatcaatt tcaatgcctt cttgttctag tcgcttgata gctttccaaa ttgatgttcg
      961 gcttagggat agtttttctg cgattttttc tccgctgata tagtcggttt ctttagatag
     1021 gatttggtag acagcttggt aggatttcat aatgttgcct ttctcactag ttggtattga
     1081 gagtattctt ttcttgtatg acttggagtc tgattaaagt attgtttata agctttcgaa
     1141 aaatggagtg gatctgaaaa acctaccgag tatgcaatta ccttgatgga ctcttgggta
     1201 ttttcgagaa gttgtctagc tcggtgcatt cgaacgtaga gtaggtattc tttgggtgat
     1261 aaggtattaa attctttgaa tacgcttgat aagtagcttc tgtgaacgga tagttctttt
     1321 gctaaatctt gaattgtaag tgattgagga tagtggctat caattaatcg tttgcattca
     1381 agatagagtt ggtgggttga tgaaatattc ttttttttct gattgggagc aatagttccc
     1441 agatgaaaca tcagttcatg aagttgtccc atgatatgga gttgagctaa ttcacttgat
     1501 tttgtaatct gagcgaagcg gacaatgtct gagatgagtt ttgcagtagt ctgggtatga
     1561 caagtttcag attggatgag ataggattga tcagaaattt gagaaagagc aaaataatca
     1621 ggggatttcc ctccagtgat tcctaaccag tagtaggccc aaggttcttt actatctgct
     1681 tgataaaagg ttagttcctc tggttttaat agaaagaaat ctccttcttt taaatcaaca
     1741 attttaccct tgtaataaaa ttttccttgt cctttagtaa tgtaatgtag aacgtatgta
     1801 tcacgaatgg ctggaccaaa agagtaatta ggtgtgcatt cctcatatcc ataaaagctt
     1861 agggcaaggt cgattgttcc agtctggtat tctgaaaaaa ctaacatgtg ctacctccta
     1921 cctaacattt taccatattc ttgagacatt tttctatttt ggaaagcgat ttcaggtgat
     1981 aaaatataat caataaagtg atgaggtgaa gtaaatggga gttaggatag agaataatct
     2041 attttatgtt gagagtaaaa atctaagttt gattattgaa aatcgaaatg gctacttact
     2101 tttgaaacat ttaggaaaga ctattaagaa ctataaaggt tccaatagtg tttatgaacg
     2161 agaccatgcc ttttcaggaa atccaacggc tactaatcga acctttagtt tagatactca
     2221 gcgacagatt tttggacaac atggcttagg agattttagg aaaccaacca tacaggttca
     2281 gcatagtgta actgaagtaa cagactttcg atttgtagaa gcaaagattt taaaaggtca
     2341 gaatggtcca cagggcttac cttctccaca tagcatggac gatacagaga ctcttgtctt
     2401 aatgttagaa gattctaagg ctcaacttag tctgactttg tattatacta cttttaataa
     2461 tgatgcgact attgctagct acagtaaatt agataataat agtaatcagg aagttgtcat
     2521 ccataaagat ttttctttta tggctgattt tccagctgca gattacgaaa tagtaactct
     2581 gcagggtgct tatgctcgtg aaaagactgt tagacgtcaa caggtagaac aaggaatctt
     2641 ttcgattagc tcaaaccgag gtgcttctgg tcatgctcaa acaccagctc ttctactatg
     2701 cgaacaagga gtcacagagg atgctgggaa tgtgtttgct attcaactaa tgtatagtgg
     2761 caactttgaa gcttttgttc aaaaaaatca attgaatgaa gttcgggtgg ctattggcat
     2821 taatccagaa aacttttctt ggaagttagc ccctgaggaa tactttgaaa caccggtagc
     2881 tttagtgact cattcagatc agggattaac tggtattagt catgaaagtc agaattttgt
     2941 actgaagcac attatgctaa gtgaattttc taaaaaagaa cgtccaattc taatcaataa
     3001 ctgggaagct acttactttg actttcagag agaaaaactg ttagagttag cagatgaagc
     3061 taagaaagtt ggcattgaac tttttgtatt agatgatggt tggtttggca atcgttttga
     3121 tgataatcgt gctttaggtg attgggttgt taatgaggaa aaactgggtg gaagtctaga
     3181 aagtctgatt tcagctatcc atgaaagagg tttgcagttt ggactttggt tagaacccga
     3241 aatgatttct gtagatagtg atttgtatcg tcaacatcct gactgggcta ttcaggttcc
     3301 tgattatgag catacttatt ctcggaatca attagtactt aatcttgcca atcctcaggt
     3361 agtagaatac ttgaaaagtg tcttagatca actcctatct tatcatgaga ttgattacat
     3421 taaatgggat atgaaccgca atatcactaa gctagggaat ggattaactt atctagagac
     3481 acagatgcaa tctcatcagt acatgctggg gctttacgaa ctcgtttctt atctgacaga
     3541 gaagcacagc catattctct ttgagtcctg ctctggtggt ggtggacgaa atgatcttgg
     3601 tatgatacgc tatttcccac aagtctgggc tagtgataat actgatgcca ttgcacgttt
     3661 accaattcaa tacggttcat cctatctcta tccaaccatt tctatggggg ctcatgtgtc
     3721 agcagtaccg aatcatcaga tgggacgaat gacaccatta gaaacacgtg gccatgtagc
     3781 aatgatggga aatttgggct atgagcttga tttgacaaat ttatcagatg aagagaaagc
     3841 tacgattgct aatcaggtga acttgtataa agaattacga ccagtagttc agttaggaca
     3901 gcagtataga ctaattaatc ctgatactgc atccaatgaa gcagctgtac aatttaatta
     3961 cggaaatcaa acgattgtaa cctacgttcg cgttttatct gttgtagaga ccatggaaac
     4021 aactttaaaa ttaaaagatt tggatgaaga gggactatat aaattacagg aaaatggcga
     4081 agtttactca ggtgcagaac taatgtatgc gggcttaact gttatcttat cccaaggaga
     4141 ttttttgagt agacagtata tttttagaaa actatgatat gtactgaccc caaaagttag
     4201 atttattcta tctaactttt gaggtgcagt ttgataaggg tgtataaatt tataaaggag
     4261 gctttctaat ggaatggtat aaaaaaatcg gacttcttgc aactacaggt ttagctttgt
     4321 ttgggctcgg tgcttgctcc aactatggta aatctgcgga tgacacagtg accatcgagt
     4381 atttcaacca gaaaaaagaa atgaccaaaa ccttggaaga aatcactcgt gattttgaga
     4441 aggaaaaccc taagataaag gtcaaagtcg tcaatgtacc aaatgctggt gaagtattga
     4501 agacacgcgt tctcgcagga gatgtgcctg atgtggtcaa tatttaccca cagtccatcg
     4561 aactgcaaga atgggcaaaa gcaggtgtct ttgaagattt gagtaacaaa gactacctga
     4621 aacgcgtgaa aaatggctac gctgaaaaat atgctgtaaa cgaaaaagtt tacaacgttc
     4681 cttttacagc taatgcttat ggaatttact acaacaaaga taaattcgaa gaactgggct
     4741 tgaaagttcc tgaaacctgg gatgaatttg aacagttagt caaagatatc gttgctaaag
     4801 gacaaacacc atttggaatt gcaggtgcag atgcttggac actcaatggt tacaatcaat
     4861 tagcctttgc gacagcaaca gatggaggaa aagaagcaaa tcaatacctt cgttattctc
     4921 aaccaaatgc cattaaattg tcggatccga ttatgaaaga tgacatcaag gtcatggaca
     4981 tccttcgcat caatggctct aagcaaaaga actgggaagg tgctggctat accgatgtta
     5041 tcggagcctt cgcacgtggg gatgtcctca tgacaccaaa tgggtcttgg gcgatcacag
     5101 cgattaatga acaaaaaccg aactttaaga ttgggacctt catgattcca ggaaaagaaa
     5161 aaggacaaag cttaaccgtt ggtgcgggag acttggcatg gtctatctca gccaccgcca
     5221 aacatccaaa agaagccaat gcctttgtgg aatatatgac ccgtccagaa gtcatgcaaa
     5281 aatactacga tgtggacgga tctccaacag cgatcgaagg ggtcaaacaa gcaggagaag
     5341 attcaccgct tgctggtatg accgaatatg cctttacgga tcgtcacttg gtctggttgc
     5401 aacaatactg gaccagtgaa gcagacttcc ataccttgac catgaactat gtcttgaccg
     5461 gtgataaaca aggcatggtc aatgatttga atgccttctt taacccgatg aaagcggatg
     5521 tggattagga gtagagagac tatgaaaaaa gtattacaaa aatattgggc atgggctttt
     5581 gtggtcatcc ccctcttgtt acaagcaatt ttcttctatg tgccgatgtt tcaaggagcc
     5641 ttttacagtt ttaccaactg gacaggattg acttataact acaaatttgt cggcttaaac
     5701 aactttaagc tcctcttcat ggatccaaaa ttcatgaatg cgattggctt taccgcaatc
     5761 attacgattg ccatggtggt tggtgagatt gcactcggga tcttcattgc gcgtgtcttg
     5821 aattctaaaa tcaaaggcca aaccttcttc cgtgcttggt tcttcttccc agctgtttta
     5881 tctggtttga cagtggcttt gatcttcaag caagtcttca actacggtct tccagcgatt
     5941 gggaatgccc ttcatattga atttctccaa accagtcttt tagggactaa gtggggagca
     6001 atctttgcgg ctgtctttgt ccttctttgg caaggggtgg ctatgcccat catcatcttc
     6061 ctagctggtt tgcaatctat tccaactgag attacagagg cagcaaggat tgatggtgcg
     6121 actagcaagc aagttttctg gaacattgaa ttgccttact tgctaccaag tgtctctata
     6181 gtctttatcc tagccctaaa aggtgggctg actgcctttg accaagtctt tgccatgacc
     6241 ggtggtggtc caaacaatgc cacaacctca cttgggctct tggtttataa ctatgccttt
     6301 aaaaacaacc aattcggtta tgccaatgcc attgccgtaa tcttgttctt cttaattgta
     6361 gtgatttcga tcatccaatt gagagtatct aagaaatttg aaatttaaga ggagaagcat
     6421 gatgaaacaa gatgaaagaa aagccctgat tggcaaatac attctattga ttctaggatc
     6481 ggttctgatt ttagtgccgc tccttgctac cctctttagt tccttcaaac ccactaagga
     6541 tattgtagat aatttctttg gttttccaac caacttcaca tgggacaact ttagccgtct
     6601 cttagctgat gggattggag gctattattg gaactctgtc gtcatcactg tcttgtcttt
     6661 acttgcagta atgatcttta tccctatggc agcctactcc atcgctcgca atatgagtaa
     6721 aagaaaagcc tttaccatca tgtataccct cttaatcctc ggaatcttcg tacctttcca
     6781 agtcatcatg attccgatta cggttatgat gagtaaactc ggtttggcta atacctttgg
     6841 tttgatcttg ctctacttga cctatgcgat tccacagacc ctctttctct atgttggcta
     6901 tatcaaaatc tcgattccag aaagtctgga tgaagcagca gagatcgatg gggctaatca
     6961 atttacaacc tatttccgca tcatcttccc aatgatgaaa ccgatgcatg cgacaaccat
     7021 gatcatcaat gccctttggt tctggaatga cttcatgttg ccactccttg tcttgaaccg
     7081 ggattccaaa atgtggactc tgcctttgtt ccaatacaac tacgcaggcc aatatttcaa
     7141 cgactacgga ccaagctttg cctcttacgt ggtcggcatt atcagtatca ccattgtcta
     7201 tctcttcttc caacgccata tcatttcagg aatgagcaac ggggcagtga agtaatactc
     7261 aatgaaaatc aaagagcaaa ctaggaagct agccgcaggc tgtacttgag tacggtaagg
     7321 tgaagctgac gtggtttgaa gagattttcg aatagtataa gaagagctca tggaatctat
     7381 agtctttgac acagaggaag agttcagctt gttgataatt ctatgtagaa aacaagaaag
     7441 tagtactttg tttatcacag tctatattag tacgagtttg atgataaata gagatttaaa
     7501 atgaatttta tttatgaagc cagatcatat gagacttggc ttcaattaga aaaaggagag
     7561 taatgatgcc aattcaaaat aaaaccatgt tgattaccta ttctgatagc cttggaaata
     7621 atcttaaaga cttatatgat aatttggaag agcattttcg agatgctatt ggaggagttc
     7681 accttttacc atttttccca tcaacaggtg atcgtggatt tgcgccagtt gactacgacg
     7741 aagtggactc agcttttggt gattgggagg atgttaagcg tttaggtgag agatattatc
     7801 ttatgtttga ttttatgatt aatcatattt ctcgtcaatc caagtattat aaggactatc
     7861 aagaaaaaca tgaagccagt gaatttaaag ctctcttttt aaactgggat aagttttggc
     7921 cagaaaaccg ttcgacacag tctgatgtag atttaattta caagcgtaag gatcgtgcac
     7981 caaagcaaga gattgtgttt gaagatggtt cagtggaaca tttgtggaat acctttggtg
     8041 aggagcagat tgatcttgat gtgaccaaag aagtaactat ggaatttatc cgtaagacca
     8101 ttcagcactt ggcaagtaat gggtgtgatt tgattcgtct agatgccttt gcttatgcag
     8161 tgaagaaatt ggatactaat gatttctttg tggaaccaga tatttgggat ttattggaca
     8221 aagttcgaga tatcgctgct gagtatggga cagagctttt acctgagatt catgaacact
     8281 attcgattca gtttaaaata gcagaccatg attactatgt ttatgatttt gctcttccaa
     8341 tggtgacact ttatactctt tacagttcca gaacagagcg tttggctaag tggttaaaga
     8401 tgagcccgat gaagcaattt acgacgctag atacccatga tgggattgga gtagtagatg
     8461 tcaaggatat cctgaccgat gaggagattg actatgcttc aaatgaactc tataaggttg
     8521 gagccaatgt caaacgtaag tactctagtg ccgagtataa caacttagat atctaccaaa
     8581 tcaattcaac ctactattca gcgcttggag atgatgatgt caagtatttt ctcgctcgtc
     8641 taattcaagc ttttgcccca ggtattcctc aggtttacta tgtgggtcta ttagcaggca
     8701 agaatgactt gaaattatta gaagaaacta aagaaggtcg aaatattaat cgtcattact
     8761 atagcaacga ggaaatagca aaagaagtgc aacgacctgt tgtgaaggcc cttctcaatc
     8821 tattttcttt ccgtaaccgt tcagaagcct ttgatctaga agggactact gagatagaga
     8881 caccaacagc tcacagcatt gtaatcaaac gtcaaaataa agataagtcc gtaacagcag
     8941 tagtagaaat tgatttgcaa aatcagactt atcgggtaat tgagaatgga gttgaagtat
     9001 cattttgaag ccttgatatg gtggataaaa tagggttttt attttggaaa acgtttcctt
     9061 tgttttcaaa ttgctaaaaa agtggtacaa tataggatag cttactatta tctgaatcag
     9121 ctgatttgga gagaaaggat tcattttgaa atcaataggc tttattgaaa agctgaaggg
     9181 gttgtctagt aaagagctga ttttattggg aattatccta agtatctttt taccctttta
     9241 tctttttgta gttgtactct gtttatatat tatcagtttg atttttacag gagacatgaa
     9301 aagtattctt cagaaaatgg gggagcatcc gatgctgctt ctttttctta gctatagtac
     9361 tgttatatcc attcttgcac aaaattggat gggtcttgtg gcttcagtag gaatgtttct
     9421 atttactatt ttctttttgc actatcagtc gattttatcc cataaattct ttcgattgat
     9481 tttgcagttc gtcttgtttg gtagtgtctt gtcagctgct tttgccagtt tagaacattt
     9541 ccaaattgtg aagaaattta actatgcttt tctttcaccc aatatgcagg tgtggcatca
     9601 gaaccgggca gaagtgacct tctttaatcc taattattat ggaattattt gttgtttctg
     9661 tattatgatt gctttctatc tgtttacaac gaccaagttg aattggttga aagtattctg
     9721 tgtgattgca ggctttgtta atctctttgg tttgaacttt actcaaaatc gaactgcctt
     9781 tcctgctatt atcgctggag caattatcta tctctttacg actattaaaa actggaaggc
     9841 cttttggctt agtattgggg tcttcgcgat tggtttgagt ttcctctttt ctagtgattt
     9901 gggagttcga atgggtactt tagactcttc tatggaagaa cgcatttcta tctgggatgc
     9961 tgggatggcc ttgtttaagc aaaatccttt ttggggtgaa gggccattga cctatatgaa
    10021 ctcttatcct cggatacatg ctccttatca tgaacatgcc cacagtcttt atattgatac
    10081 gattctgagt tacggaattg tagggactat tttattagtt ttgtcttctg ttgctcctgt
    10141 tcgcttgatg atggatatga gtcaggagtc ggggaaacgt ccgattatcg gcctttatct
    10201 atctttcctt acagtggttg ctgtgcacgg aatttttgac ttggctctct tctggattca
    10261 gtcaggcttt attttcttgc tagttatgtg cagtattcca ttggagcatc gaatgttggt
    10321 atcggacatg acggattaag ttttataagt ttgaaatctt ctaccttggg taggagattt
    10381 tttatacgat gggcatgtcg tgcatctgag gtgtaagtcc tctgtagggc tccactaccg
    10441 gtgaatacaa tagcgctccc caagcctgac tatcgtgagg tagcggatta aaatggtctg
    10501 gggatagatc gttttaagtc tgacgcagga aataagaatt gtcagagaaa gcaatagtga
    10561 aatcgtaggc tatgaataga aacgtgatag cgaggagcat atagtggata aggtagcgag
    10621 aaattttaaa gtataaaaga tagtcttaat ctatatatgt aaatcacaaa agtaattgaa
    10681 ttcgggataa ggttggtgtg aaaagacata gtaaactgaa tcttgaatac tagaatgata
    10741 cttctacaat attgttataa tttaatttga attccaaaat cacattgttc cgtccgtatt
    10801 tcattttact gtatgtcttc ctaaactcca aagtttctgc gtcaaatttc ctattttcac
    10861 tgtagtcttt gacacttcga acgacaattt gaatcatata ttcaaaaaaa atgaaaaatt
    10921 tgtgccaaca agcctaaata ttctgaaaat ttgtttgatt tttaatggat aatgtgatat
    10981 aatggttgtc aatctataat tcacataaac agaggagtag aaaatgaaaa aaaatcgtgt
    11041 atttgctaca gcaggtcttg ttttattagc agcaggtgta cttgcagcat gcagttcttc
    11101 aaaatcatct gattcatcag cccctaaagc ttatggctat gtttatacag cagacccaga
    11161 aaccttggac tacctgattt caagtaaaaa tagtacaaca gtagtgactt caaatgggat
    11221 tgatggttta ttcactaacg ataattacgg taatctttct cctgcagttg cagaggattg
    11281 ggaagtctct aaggatggtt tgacctacac ttataagatt cgtaaagggg ttaaatggtt
    11341 tacctctgat ggagaagaat atgcagaggt gacggctaaa gatttcgtga acggtttaaa
    11401 acacgcagca gataaaaaat cagaagctat gtatttagct gaaaattcgg ttaaaggctt
    11461 ggcagattat ctatcaggaa cttcaacaga tttttcaaca gttggtgtca aggcggttga
    11521 tgattatacg ttacaataca ctttgaacca gcctgaaccg ttctggaact ctaagttgac
    11581 ctattctatt ttctggcctc tgaatgaaga attcgaaaca tcaaaaggaa gcgattttgc
    11641 taaaccaaca gatccgacat ccttgcttta taatggtcca ttcttgttga aagggttgac
    11701 tgcaaaatct tctgtagagt ttgtaaaaaa tgagcaatat tgggataaag aaaatgtcca
    11761 cctagatact atcaatctag cttactatga tggatcagat caggagtcgc tagagcgtaa
    11821 cttcactagt ggagcttata gttatgcccg tctttaccct accagctcca actattctaa
    11881 ggttgcagaa gaatacaagg acaatatcta ttacacacaa tcaggctctg ggattgctgg
    11941 tctgggtgtg aatattgatc gccaaagtta caactatact tctaaaacta cagattcaga
    12001 gaaagtagct actaagaagg cattgcttaa caaagatttc cgtcaagcct tgaattttgc
    12061 tcttgatcgc tcagcttact cagctcaaat caatggtaaa gatggagcag ctttagcagt
    12121 tcgtaattta tttgtaaaac cagactttgt ttcagctggt gagaagacct ttggtgattt
    12181 agtcgctgct caacttcctg cttatggtga tgagtggaaa ggtgtgaatt tagctgatgg
    12241 gcaggatggt ttattcaatg ctgacaaggc caaggcagag tttgcgaaag ctaagaaagc
    12301 tttagaagca gacggcgttc agtttcctat tcatctggac gttccagtag accaagcatc
    12361 aaaaaactat atatctcgta ttcagtcctt taaacaatct gtagaaacag ttcttggtgt
    12421 tgaaaatgtc gttgttgata ttcaacaaat gacaagtgat gaattcctta atattactta
    12481 ctatgctgcc aatgcttcat ctgaggattg ggatgtatca ggaggagttt catgggggcc
    12541 agactatcaa gacccatcta cttacctgga tattttaaaa acaactagca gtgaaactac
    12601 aaaaacatat ttaggatttg ataatccaaa tagcccttca gtagttcaag ttggtttgaa
    12661 agaatacgat aaattagttg atgaagctgc caaagagaca agcgacttga atgtccgtta
    12721 tgaaaaatat gcagcggctc aagcatggtt gacagatagt tcactcttta ttcctgctat
    12781 ggcttcttct ggtgcagcac cagtgctttc acgaattgtt ccatttactg gagcttctgc
    12841 gcaaacaggc tctaaggggt cagatgttta cttcaaatat ttgaaattac aagataaagt
    12901 ggtgactaag gaagagtatg aaaaagctcg tgaaaaatgg ttgaaagaaa aagctgaatc
    12961 aaatgagaaa gctcaaaaag aattggcaag tcatgtgaag taaataattt ctaatagaac
    13021 tttctctcca tgaggagaag gttcttttgg gatttttaaa ggaaataata tgaaaaaata
    13081 tatttttatg cgtgttttgc ggtcattggt ttcgattttc ttagtaacga ctttgaccta
    13141 cacgattatc tataccttgg ttcctcgaaa attgattttc aagcaggatc ctaactataa
    13201 taaaattgcg acaacggctg ataaacgtga taactatgaa aatactgtgt ttgagcgtat
    13261 gggctacatt gagtattacg atactaaaga gttgcaagaa aaggcaagta gcatggattc
    13321 ttctgtaaca gtagaagcaa atgcgaccaa taaagctatt tatgaaaagt acatcaatca
    13381 attaggtcat ggttggactt tgggagaatt tactgaaagt ggtcaattct atgctactcg
    13441 tgaaattcca atttttgaac gtgtttttca cttctatgct aacttgattg acattgacca
    13501 tacaaataaa atccaagacc ctgaaaatcc agacttgaaa cgctaccttc gttttgaaaa
    13561 tgatccagct atcggatggt cattggtcgg ttcaggaact aaacataaat atctcttgta
    13621 ctttaacagt cagttcccat ttgttcatca aaactttgtg aacttgaatt taggtgactc
    13681 ttacccaacc tatgctaata caccagttct tcaggttatt actcaaggtc aaggacaaac
    13741 caaaactgcc caagttcagt tcccaacagg taagaaaacg tcttctgtaa atatttactc
    13801 aagaacctac aagtcaccta gtcaggctga ctctcgtgaa gtagctagct atgggaaaga
    13861 tgatccttat acagcgactg aaagtaatta ccaatatcca tctatgattg tcagctctgc
    13921 tattactggt ttgattggtt tggttcttgc ctatgctctt gccgtgccac ttggttcagc
    13981 catggctcgt ttcaagaaca cttggattga tagcctctca acagggactt tgaccttctt
    14041 gcttgctctt ccaacgattg ccttggttta catcgttcga ttgattggat catctattgc
    14101 ccttccagat tcattcccta tcttgggagc tggagattgg cgttcttacg ttttaccagc
    14161 agtcatcctt ggtttgttgg gtgctcctgt tacagccatt tggattcgtc gttacatgat
    14221 tgacttgcaa tctcaagact ttgttcgttt cgctcgtgca aaaggtttgt ctgaaaaaga
    14281 aatttcaaac aaacacatct ttaaaaatgc catggttccg ctggtttcag gaattcctgg
    14341 tgccattatt ggggttatcg gtggtgcaac ccttactgaa acagtcttcg ccttcccagg
    14401 tatgggtaaa atgttgattg actctgtaaa agcatctaat aactctatgg tcgttggtct
    14461 tgtcttcatc tttacatgta tttctatctt ctcacgtctt ttgggagata tttggatgac
    14521 tattattgac ccacgtatta aattgactga gaaaggaggc aaataatgtc tacaatcgat
    14581 aaagaaaaat ttcagtttgt aaaacgtgac gattttgcct ctgaaactat tgatgcgcca
    14641 gcatattctt actggaaatc agtgtttaaa caatttatga agaaaaaatc aactgtagtc
    14701 atgttgggaa tcttggtagc catcattttg ataagtttca tctacccaat gttttctaag
    14761 tttgatttca atgatgtcag caaggtaaac gactttagtg ttcgttatat caagccaaat
    14821 gcggagcatt ggttcggtac tgacagtaac ggtaaatcgc tctttgacgg tgtctggttc
    14881 ggagctcgta actccatcct catttctgtg attgcgacag tgattaactt ggttatcggt
    14941 gtttttgtcg gtggtatttg gggtatttca aaatcagttg accgtgtcat gatggaagtt
    15001 tacaacgtca tctcaaacat cccacctctt ttgattgtta ttgtcttgac ttactcaatc
    15061 ggagctggat tctggaatct gatttttgcc atgagcgtaa caacatggat tggtattgcc
    15121 ttcatgatcc gtgtgcaaat cttgcgctat cgtgacttgg aatacaactt ggcgtcacgt
    15181 actttgggaa caccaacctt gaagattgtt gccaaaaata tcatgcctca attggtatct
    15241 gttattgtga caaccatgac tcaaatgctt ccaagcttta tctcatacga agccttcttg
    15301 tctttcttcg gtcttggatt accgattaca gtgccaagtt tgggtcgttt gatttcggat
    15361 tattcacaaa acgtaacaac caatgcttac ttgttctgga ttccattgac aacccttgtc
    15421 ttggtatcct tgtccctttt cgtagttggt caaaacttag cggatgctag tgatccacgt
    15481 acacatagat aggagtagaa atgacaaaag aaaaaaatgt aattttgact gctcgcgata
    15541 ttgtcgtgga atttgacgtt cgtgacaaag tattgacagc cattcgcggc gtttcccttg
    15601 aactagttga aggagaagta ttagccttgg taggtgagtc aggatcaggt aaatctgttt
    15661 tgacaaagac cttcacaggt atgctcgaag aaaatggtcg tattgcccaa ggtagtattg
    15721 actaccgtgg tcaggacttg acagctttat cttctcacaa ggagtgggaa caaattcgtg
    15781 gtgctaagat tgcgactatc ttccaggacc caatgactag tttggacccc attaaaacaa
    15841 ttggtagtca gattacagaa gttattgtaa aacaccaagg aaaaacagct aaagaagcga
    15901 aagaattggc cattgactac atgaataagg ttggcattcc agacgcagat agacgtttta
    15961 atgaataccc attccaatat tctggaggaa tgcgtcaacg tatcgttatt gcgattgccc
    16021 ttgcctgccg acctgatgtc ttgatctgtg atgagccaac aactgccttg gatgtaacta
    16081 ttcaagctca gattattgat ttgctaaaat ctttacaaaa cgagtatcat ttcacaacaa
    16141 tctttattac ccacgacctt ggtgtggtgg caagtattgc ggataaggta gcggttatgt
    16201 atgcaggaga aatcgttgag tatggaacgg ttgaggaagt cttctatgac cctcaccatc
    16261 catatacatg gagtctcttg tctagcttgc ctcagcttgc tgatgataaa ggggatcttt
    16321 actcaatccc aggaacacct ccgtcacttt atactgacct gaaaggggat gcttttgcct
    16381 tgcgttctga ctacgcaatg cagattgact tcgaacaaaa agctcctcaa ttctcagtat
    16441 cagagacaca ttgggctaaa acttggcttc ttcatgagga tgctccaaaa gtagaaaaac
    16501 cagctgtgat tgcaaatctc catgataaga tccgtgaaaa aatgggattt gcccatctgg
    16561 ctgactagga ggaaggaaat gtctgaaaaa ttagtagaaa tcaaagattt agaaatttcc
    16621 ttcggtgaag gaagtaagaa gtttgtcgcg gttaaaaatg ctaacttctt tatcaacaag
    16681 ggagaaactt tctcgcttgt aggtgagtcc ggtagtggga aaacaactat tggtcgtgct
    16741 atcatcggtc taaatgatac aagtaatgga gatatcattt ttgatggtca aaagattaat
    16801 ggtaagaaat cgcgtgaaca agctgcggaa ttgattcgtc gaatccagat gattttccaa
    16861 gaccctgccg caagtttgaa tgaacgtgcg actgttgatt atattatttc tgaaggtctt
    16921 tacaatcacc gtttatttaa ggatgaagaa gaacgtaaag agaaagttca aaatattatc
    16981 cgtgaagtag gtcttcttgc tgagcacttg actcgttacc ctcatgaatt ctcaggcggt
    17041 caacgtcaac gtatcggtat tgcccgtgcc ttggtcatgc aaccagactt tgttattgca
    17101 gatgagccaa tttcagcctt ggacgtttct gtacgtgccc aagtcttgaa cttgctcaaa
    17161 aaattccaaa aagagctcgg cctgacctat ctcttcatcg cccatgactt gtcggttgtt
    17221 cgctttattt cagatcgtat cgcagttatt tacaagggtg ttattgtaga ggttgcagaa
    17281 acagaagaat tgtttaacaa tccaattcac ccatatactc aagccttgct ttcagcggta
    17341 ccaatcccag atccaatctt ggaacgtaag aaggtcttga aggtttacga cccaagtcaa
    17401 cacgactatg agactgataa gccgtctatg gtagaaatcc gtccaggtca ctatgtttgg
    17461 gcgaaccaaa ccgaattggc acgttatcaa aaaggactaa actagtaatg gttttataat
    17521 ttccatgtca atttttatgg aaattaggct ctttgtcaac tgtagtgggt tgaagtcagc
    17581 taagcttgag aaaggacaaa tttcgtcctt tcttttttga tgttcaaagc gataaaaatc
    17641 cgttttttga agttttcaaa gtttcgaaaa ccaaaggcat tgcgcttgat aagtttgatg
    17701 agattattgg tcgcttccag tttggcatta gaatagtgta gttgaagggc gttgataatc
    17761 ttttctttat ctttgaggaa ggttttaaag acagtctgaa aaataggatg agcctgctta
    17821 agattgtcct caataagtcc gaaaaatttc tctggttcct tattctggaa gtgaaacagc
    17881 aagagctgat agagctgata gagctgatag tggtgtttca agtcttgtga ataactcaaa
    17941 agcttgttta aaatctcttt attggttaag tgcatacgaa aagtaggacg ataaaatcac
    18001 ttatcactca gtttacggct atcctgttga atgagtttcc agtagcgctt gatagccttg
    18061 tattcatgg
//
LOCUS       DAVKWZ010000039        17423 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000039 DAVKWZ010000000
VERSION     DAVKWZ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17423
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.039"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            127..387
                     /locus_tag="VW854_001423"
     CDS             127..387
                     /locus_tag="VW854_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262646.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADMHRFVIELEPVKSTILQNDDISLDQNEIFEILKNFFDG"
     gene            complement(1152..1551)
                     /locus_tag="VW854_001424"
                     /pseudo
     CDS             complement(1152..1551)
                     /locus_tag="VW854_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1676..2134
                     /locus_tag="VW854_001425"
     CDS             1676..2134
                     /locus_tag="VW854_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEV0262647.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            2147..2755
                     /locus_tag="VW854_001426"
     CDS             2147..2755
                     /locus_tag="VW854_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262648.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            2826..3158
                     /locus_tag="VW854_001427"
     CDS             2826..3158
                     /locus_tag="VW854_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262649.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDHLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            3169..4584
                     /gene="ply"
                     /locus_tag="VW854_001428"
     CDS             3169..4584
                     /gene="ply"
                     /locus_tag="VW854_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEV0262650.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWDELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            5465..6181
                     /locus_tag="VW854_001429"
     CDS             5465..6181
                     /locus_tag="VW854_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV0262651.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            6305..6775
                     /locus_tag="VW854_001430"
     CDS             6305..6775
                     /locus_tag="VW854_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEV0262652.1"
                     /translation="MDKPMLVFKRFGHQIYLMVQKEAKRCGIEFMGGPQGQVVRFLDN
                     REKNQDLVLIKDIEQELNITKSVASNLVKRIVQNGLVELEASPVDKRAKFVRLTDKAR
                     SQMQQVKAFFERIDKQLMEDIDEDELLIFEKVLGQLQANIKGIGGENKEISQKN"
     gene            6791..7612
                     /locus_tag="VW854_001431"
     CDS             6791..7612
                     /locus_tag="VW854_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0262653.1"
                     /translation="MILLAILFTCFSVYLELEVPTYISKITDLLGSQETNLDELWQPA
                     SMMMGMSFLAFLSVVAVGFFASRVAASYTSRLRSDIFNRVLDYSQTEIKKFSIPSLLT
                     RTTNDITQVQMLITMGLQVVTRGSIMAIWAIGKILGHSEYWLWAVLVAVIINVLMTTV
                     LMTLAFPKQSLIQGLTDKLNSITRESLTGIRVVRAYNAEDYQNEKFAAVNDELTRLNL
                     FVNRLMAILNPIMMGISSGLGVAIYWIGAYVINDAAPIARLPLFSDMIVFMSYAM"
     gene            7619..8650
                     /locus_tag="VW854_001432"
     CDS             7619..8650
                     /locus_tag="VW854_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV0262654.1"
                     /translation="MGFLLMGALFIVLPRTMVSAKRINQVLDLHSSIQNPVQVQLTDE
                     NFKGQVEFKDVTFRYAANSEAVIEHVSFKAETGQTVAFIGSTGSGKSTLVNLIPRFYD
                     VSAGEILVDGVNVQDYDFSATAHAGQKVAIVGPTGAGKTTIVNLLMKFYEIDKGSIRI
                     DGVDTKAMTRSEVHDAFSMVLQDTWLFEGTIRDNLIYNQIGISDERMMEASKAVGIHH
                     FIMTLPDGYDTILDDTVTLSVRQKQLLTIARALLKDAPLLILDEATSSVDTRTEELIQ
                     KAMDRLMEGRTSFVIAHRLSTIRNADLILVMKDGNIIEQGNYEELMAQGGFYADLYNS
                     QFTEDEAEE"
     gene            8716..9078
                     /locus_tag="VW854_001433"
     CDS             8716..9078
                     /locus_tag="VW854_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 family protein"
                     /protein_id="HEV0262655.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDSLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            9075..9578
                     /locus_tag="VW854_001434"
     CDS             9075..9578
                     /locus_tag="VW854_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV0262656.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVTLSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSAAIISMACLHASLPIGLVCLNFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLL"
     gene            9692..10138
                     /locus_tag="VW854_001435"
     CDS             9692..10138
                     /locus_tag="VW854_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0262657.1"
                     /translation="MKLRIEIDGNLEETEIVIKAAALTDEIADLQRLLQESKSPRLIF
                     YKGTGEYYLDLSEILFFETEGSKIYAHTQKNAYEVRLKLYELEAILPRYFSRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            10143..10817
                     /locus_tag="VW854_001436"
     CDS             10143..10817
                     /locus_tag="VW854_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262658.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLGGHIWPLIGIAFFAYQ
                     SVGALLRRHLTSASFTALVALMIANHFYGILPIPNQTLIWAGVLIVLGVNMLTHSNRT
                     WNGKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLTIRGDVAFGKL
                     EIVYVK"
     gene            10978..11169
                     /locus_tag="VW854_001437"
     CDS             10978..11169
                     /locus_tag="VW854_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HEV0262659.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            11506..11805
                     /locus_tag="VW854_001438"
     CDS             11506..11805
                     /locus_tag="VW854_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEV0262660.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            11802..12119
                     /locus_tag="VW854_001439"
     CDS             11802..12119
                     /locus_tag="VW854_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEV0262661.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            12135..12761
                     /locus_tag="VW854_001440"
     CDS             12135..12761
                     /locus_tag="VW854_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEV0262662.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLNVERKGKVARVFLKENGE
                     TVAWNIFEVSSLFEIAERGQVFLSDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKIVQIVAGAPNARVGLKTIVALPGAMMPKGNFIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            12794..13555
                     /locus_tag="VW854_001441"
     CDS             12794..13555
                     /locus_tag="VW854_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV0262663.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEALKA
                     EFAETFPNQTVWTFLLDVTDMTMVKTVCSDILETIGQIDILVNNAGLALGLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMVKANQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMVHKK"
     gene            13633..14028
                     /locus_tag="VW854_001442"
     CDS             13633..14028
                     /locus_tag="VW854_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV0262664.1"
                     /translation="MYNKVIMIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            14184..14468
                     /gene="groES"
                     /locus_tag="VW854_001443"
     CDS             14184..14468
                     /gene="groES"
                     /locus_tag="VW854_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEV0262665.1"
                     /translation="MLKPLGDRVVLKVEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            14484..16106
                     /gene="groL"
                     /locus_tag="VW854_001444"
     CDS             14484..16106
                     /gene="groL"
                     /locus_tag="VW854_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEV0262666.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            16244..16999
                     /locus_tag="VW854_001445"
                     /pseudo
     CDS             16244..16999
                     /locus_tag="VW854_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567550.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
BASE COUNT         5216 a         3139 c         3935 g         5133 t
ORIGIN      
        1 caaaccacgt cagcttccat ctacaacctc aaaacagtgt tttgagcagc ctgcggctag
       61 cttcctagtt tgctctttga ttttcattga gtattaatta tttttaaggc taaagcttgg
      121 ctggatatga gggaacgctc tgctacaggt gctcaaggtt taagtaagtc cattaaaaag
      181 catttgaatg accttacccg tttgacagct tccttgctag gagatgaaaa gttatcggct
      241 ataacatcaa gtagtgcggt aaaagcagac atgcaccgct ttgtgataga attagagcct
      301 gtgaagtcaa ctattcttca aaatgatgac atttcattag atcaaaatga aatttttgaa
      361 attctgaaaa attttttcga tggttaaaat aattgtagcg agatggctat attgaattcg
      421 tttatatctg gaaactagaa aaaacttcaa tttcaggaga aaatgaagtc aatcttccca
      481 caatcaaacg tatagtatca aggtttttca agacctgata ttatgcgttt tttgcttttc
      541 aaaacttttt gcccagtttt tgtttttatc ctctagtcac ttgatttgtt tcaggtggtt
      601 ttttagtata gtaaaatgaa acgagaacag gacaaattga tcaggacagt caaatcgatt
      661 tctaatcaat gttttagaag cagaagtgta ctattctagt ttcaatctac tatagttaaa
      721 tctgcggtca agtctactgg tggatctatg attgtaatac tcttccaaaa tctcatcaac
      781 cacgtcagtc ttgccttgca gtctgtatct tactgaccaa gctagtgatg gacttagaat
      841 aggtgatttg gagcgtccta ttggctagga aatgctgatc atagtccttt gctgaggcta
      901 gggtgtttca acattcaaca ctcaactggt tgatctagtt gataggaagg gaattactat
      961 aaaatactca ggcttccatc atattttttg aaacgattgt gtaatcaaaa tgtaccaata
     1021 ttgtagtatt ggtacagaag atgttgtgaa tggataaata tatcataact gctgtctctc
     1081 tatgtaaaaa gaaggttgta tcaaaaagat ttcatatgtc tgtgcatata tataatagac
     1141 tttctgcaag actagaatcc tagttcatga tttataatgc cagcaatcaa attcattcgt
     1201 aatccaaagc gtttacgatg atttcgatag gttgttgaaa acattttaaa cgtttctact
     1261 ttggcaaaga tgttctcaac cttgcttctc tccttagata gcgcatggtt ataggtttta
     1321 tcttcaactg ttagcggctt gagtttgctg gatttacgtg gaatttgtgc ttgaggatat
     1381 atcttcatga gcccttgata accactgtca gccaagattt taccagcttg tccgactttt
     1441 ttgataagct gtttttaata cagctaatat ctcttcaaaa gtggtacgct gaacactaac
     1501 aagacgctta aatcgtgcat cagttagttg tttacttgct tcataattca tagaactact
     1561 ataccatatt ttgtttcgca ggaagtctaa taagttatag tccagttttt actatacttt
     1621 gatattcaaa tatgttatac tatggttatc aaagtaaatg aaaggagtta cgaatatgac
     1681 gccagaagaa atgtacctga cagagcgatt agacgtacag atagctcatt ttttaaagaa
     1741 aagcgttcaa catcgtaggc gctataaggt attaaaaata acagaaatcg tggcaggttt
     1801 cctcatagct gtcttttgtg ctattcctat gccaggtgat cgctaccgtt tgatttcggt
     1861 tgccttatcc agtctcggct tgctgtgtga ggggattatc aatttgtata atgcaaagga
     1921 aaattggatt tcttaccaaa aaactgcgca actcctggag aaagaaaaat tcctctatca
     1981 atgccaaacg gagaaatatg caggaaagac caaggctttt gccctatttg tcaagacatg
     2041 cgaaggtctt atctcagagg agattaacca gtgggaaagt atccagtcaa aagaagtggc
     2101 agctagtgca gatgctccag ttaaaaaaga gtaggaggta gaggaaatgt ctcaatccag
     2161 ttacctgtcg cccttgctct ggttaaaaaa agaagccgat aaggaaaaga tgagcgcgac
     2221 ccagtgccag atatttttct tttactatca aatgtttgag ctcttatttg ctagagaaag
     2281 cgacatgaaa gacttatgtc tgggaacgaa aggtttttat ttctcgcagt tagagaaaaa
     2341 tttgctttct ggagtttccc gatttctaaa aaacttggag gggaaagtaa ctctcaaggc
     2401 taaccaagaa gtatcagctc gcaaagccct ttttctagcc ttgacaacta gccaatcaga
     2461 ttggcaggag ttagctcctg tttttgattt ttatcagact atcgggaggc ttgaaaatcc
     2521 ttctctcttg agttctcagg acagacaaca tctgatgtgg atttaccagt cagctttgga
     2581 gaaggattat attgtcaagg ttattggcga caagcatttt gtattgaaga gacaagatgc
     2641 tactaaattg acagcgcgcc aaactcaaac tttggaaatt ctgagtcaat cagaagactt
     2701 ggtcaatcct gtctatgtta cattaggaga aaagggggtg ctcttgcttg attaagagag
     2761 gagatgttgt agctctttat ttgccttttc cgactattag tagcgatttg gctgtgaaga
     2821 atcatatgta tatctgtatt gacaacagca tgactaaaaa caaagagttg gttaaaaatc
     2881 agaccttcaa accagctctc ttgaccagac gtttggtcaa gaactttatg atagaagagc
     2941 cggatctagc tcgtaatcct tttacaagac caaccttgat tgacttagat aaggtattta
     3001 tgttggataa tacggttatt ccgacttctt atctagccag acggcgacgc aatgtctcag
     3061 aagaattgta cgaggaaatt ttggatcact tagtccaacc acggctgatt tcgctgaaca
     3121 agtctgagtt tatgcaactc aatccaggaa cttattagga ggtagaagat ggcaaataaa
     3181 gcagtaaatg actttatact agctatgaat tacgataaaa agaaactctt gacccatcag
     3241 ggagaaagta ttgaaaatcg tttcatcaaa gagggtaatc agctacccga tgagtttgtt
     3301 gttatcgaaa gaaagaagcg gagcttgtcg acaaatacaa gtgatatttc tgtaacagct
     3361 accaacgaca gtcgcctcta tcctggagca cttctcgtag tggatgagac cttgttagag
     3421 aataatccca ctcttcttgc ggtcgatcgt gctccgatga cttatagtat tgatttgcct
     3481 ggtttggcaa gtagcgatag ctttctccaa gtggaagacc ccagcaattc aagtgttcgc
     3541 ggagcggtaa acgatttgtt ggctaagtgg catcaagatt atggtcaggt caataatgtc
     3601 ccagctagaa tgcagtatga aaaaatcacg gctcacagca tggaacaact caaggtcaag
     3661 tttggttctg actttgaaaa gacagggaat tctcttgata ttgattttaa ctctgtccat
     3721 tcaggcgaaa agcagattca gattgttaat tttaagcaga tttattatac agtcagcgta
     3781 gatgctgtta aaaatccagg agatgtgttt caagatactg taacggtaga ggatttaaaa
     3841 cagagaggaa tttctgcaga gcgtcctttg gtctatattt cgagtgttgc ttatgggcgc
     3901 caagtctatc tcaagttgga aaccacgagt aagagtgatg aagtagaggc tgcttttgaa
     3961 gctttgataa aaggagtcaa ggtagctcct cagacagagt ggaagcagat tttggacaat
     4021 acagaagtga aggcggttat tttagggggc gacccaagtt cgggtgcccg agttgtaaca
     4081 ggcaaggtgg atatggtaga ggacttgatt caagaaggca gtcgctttac agcagatcat
     4141 ccaggcttgc cgatttccta tacaacttct tttttacgtg acaatgtagt tgcgaccttt
     4201 caaaatagta cagactatgt tgagactaag gttacagctt acagaaacgg agatttactg
     4261 ctggatcata gtggtgccta tgttgcccaa tattatatta cttgggatga attatcctat
     4321 gatcatcaag gtaaggaagt cttgactcct aaggcttggg acagaaatgg gcaggatttg
     4381 acggctcact ttaccactag tattccttta aaagggaatg ttcgtaatct ctctgtcaaa
     4441 attagagagt gtaccgggct tgcctgggaa tggtggcgta cggtttatga aaaaaccgat
     4501 ttgccactag tgcgtaagcg gacgatttct atttggggaa caactctcta tcctcaggta
     4561 gaagataagg tagaaaatga ctaggagagg agaatgcttg cgacaaaaag aggcgatgat
     4621 ctctctgcgg atatctgaaa gtttatctct tgcctagcga tttccattgg agtatgctcg
     4681 tgatgttaaa atgaatctat caaacagaat gtgtaaaaaa gtaaaaaggg tagccaattc
     4741 cttgatatac aggggattga ttatccttta cttgttttag atgattggaa attcaagaat
     4801 gacacgtatc ggaaatattt tgctgtttat atctaatacc catttatgga aaaaatgaaa
     4861 ttaaatatga acaaatcaat tgctagcaat ggtttagaag tgccagtgta ctagtctaat
     4921 ttctccatct tgggaacacg taaattatac tcttcgaaaa tctcttcaaa ccaggtcagc
     4981 cctatccgca acctcaaaac agtgttttga gcaacctgcg gctagctttc tagtttgctt
     5041 tttgattttt attgagtatt agaggtttgc ttagaaactc ttctattgat ttcttggaat
     5101 acaatagcgc ctataagaac tgttggattt caaagtattt acttgaacta tgatatgtct
     5161 ggagtagact gtcaacaaaa ggtgcagtat gattgttttt gtcgcttata tctacaaact
     5221 tcaaacagta atttgagctg actttgtcaa acttggctga aactttctat ttgcagataa
     5281 tggtctagtt tgttttttga atgtggatga gtgtctgttg ataaagatag aaaggtaaga
     5341 ccatcttagc aggtaaagtg ctatcctagc ttgatttgct aataaaatcc atttcctcta
     5401 gtgaaaatca aaaaaacttg tgttataata agaaagatta aaatgtgaaa aaaggagatt
     5461 cctaatggga cgtaaatggg ccaatatcgt agccaagaaa acggctaaag atggagctaa
     5521 ctctaaagta tatgcaaaat ttggtgtaga aatctatgta gcagctaaaa aaggtgatcc
     5581 agatccagaa tcaaactcag ctttgaaatt cgttatcgac cgtgctaaac aagcccaagt
     5641 gccaaaacac attatcgata aagcgattga taaagccaaa ggaaacacag acgaaacctt
     5701 tacagaagga cgttacgaag gttttgggcc aaatggctct atgctaattg tggatacttt
     5761 gacttctaac gttaaccgta cagcagccaa tgtccgtgca gcctttggta aaaacggcgg
     5821 aaacatgggc gcttcaggtt ctgtttctta cctttttgac aacaaaggtg ttattgtatt
     5881 tggaggtgaa gatgcggacg cagtctttga gcaattgctc gaagcggatg tggatgtgga
     5941 tgacgtagaa gcacaagaag gtacaatcac agtttacaca gcgccaactg accttcacaa
     6001 ggctatcgtt gctctccgtg agtctggtat tgaagaattc caagtgactg aattggaaat
     6061 gattcctcag tcagaagtgg aattgtcagg cgaagacctt gaaacctttg aaaaacttta
     6121 cagcgttctt gaagacgacg aagacgtcca aaagatttat acgaacgtag atgggtttta
     6181 atttaaccta tagaacatga tcctaagtga gaaaatcact tgggattttt ttaatcaaga
     6241 aaatagttct catagaaatt taattattct ccagagaact atttttattt agaaggggga
     6301 gattatggat aaaccgatgt tagtctttaa gcgttttggt catcaaattt acctgatggt
     6361 gcaaaaggaa gccaaacgtt gcggcattga atttatgggt gggccgcaag gtcaggttgt
     6421 gcgttttttg gataatcgcg agaaaaacca agacttggtc ttgattaaag atatcgagca
     6481 ggaactcaat attaccaagt ctgttgctag taatctggtt aagcgtatag tgcaaaatgg
     6541 tttggtggaa ttggaggcga gtcctgttga taagcgggct aagtttgttc gtctgacgga
     6601 caaagcacgt tctcagatgc aacaggttaa ggcttttttt gaacgcatag acaagcagtt
     6661 gatggaagac attgatgaag atgaattact gatttttgag aaagttctcg gtcaactaca
     6721 ggcaaatatc aagggaatag gaggagagaa taaagaaatt agccaaaaga attagtagaa
     6781 aagaatgggg gtgattttac tagccattct ctttacttgc ttttcggtct atctagagtt
     6841 ggaagtgccg acctatatct cgaaaattac ggatttgcta ggtagtcaag aaactaattt
     6901 agatgagttg tggcagccgg caagcatgat gatgggaatg tcctttcttg ccttcttgtc
     6961 cgtagttgca gttggatttt ttgcatcccg agtggcggct tcttatacta gtaggctgag
     7021 aagtgatatt tttaaccgag ttttggatta ctcgcagaca gagattaaga aattttcaat
     7081 tcctagcctc ttgacgcgta ctaccaatga cattactcaa gttcaaatgt tgattactat
     7141 gggcttgcaa gtggtaacgc gtggttcaat tatggctatc tgggctattg ggaagatttt
     7201 aggtcattca gaatactggc tctgggccgt acttgtggca gtgattatca acgtcctgat
     7261 gacgaccgtt ttgatgacgc tagcctttcc aaaacagtcc ttgattcagg ggctgacaga
     7321 taaactgaac agtatcactc gtgagagttt aacaggtatt cgtgtcgttc gtgcctacaa
     7381 tgcagaggat tatcaaaatg aaaaatttgc agcagtaaat gatgaattga cccgtttgaa
     7441 tttgtttgtc aaccgtctta tggctatttt gaatcctatc atgatgggga tttcaagtgg
     7501 tttgggtgta gcgatttact ggattggggc ctatgtgatt aacgacgctg ctccgatagc
     7561 gcgtctgcct ctctttagtg acatgattgt tttcatgtct tatgccatgt aggttgtcat
     7621 gggcttcctt ctcatgggag cactcttcat cgttcttccc cgaactatgg tctctgctaa
     7681 gcggattaat caagttttag atttgcattc ttctatccaa aaccctgttc aagtgcagct
     7741 gactgatgaa aacttcaaag gtcaggtcga gtttaaggat gtgaccttcc gctatgcggc
     7801 aaattcggag gcagttattg aacatgttag ctttaaagca gaaactggtc aaacagtggc
     7861 ctttattggg tcaacaggtt ctggtaaatc aactctggtc aatctgattc cacgtttcta
     7921 cgacgtgtca gcaggagaaa ttctggtgga cggtgtcaat gttcaagact atgacttctc
     7981 tgcgacagct catgctggtc aaaaggttgc cattgttggg ccgactgggg ctggtaagac
     8041 aaccattgtc aatcttttga tgaaattcta tgagattgat aagggaagta ttcgcattga
     8101 tggtgtggat accaaggcta tgacgcgttc agaagtgcat gatgcctttt caatggtctt
     8161 gcaggatacc tggctctttg aaggaactat tcgagacaat ctcatctata atcaaatagg
     8221 gattagtgat gaacgaatga tggaagctag taaggctgtg ggaattcacc actttattat
     8281 gaccttgcca gatggctatg ataccatctt ggatgacacc gtgaccttgt ctgtaagaca
     8341 aaaacaacta ttgactattg ctcgtgccct tcttaaggat gcaccgcttt tgattttgga
     8401 tgaggcgact tcttctgttg acacacggac agaggaattg atccaaaaag ccatggaccg
     8461 tttgatggaa ggacgcacat cctttgtcat tgcccaccgc ttgtcaacca tccgaaatgc
     8521 agacttgatc ttggtcatga aagatggaaa tatcatcgag caaggcaact atgaggaact
     8581 gatggcgcaa ggtggcttct acgctgactt gtacaatagt caatttacag aagatgaagc
     8641 agaagaataa atcaaaagaa ggcttgacga ccttcttttt cttatttata ctagtacaca
     8701 tagaaagaga gaagtatgag tcagaaatta tacaatatga aatttgctgc tgtctactta
     8761 gccttgattg caaaagttga gcgaaagggt ggaaaggcag agtcagttca ccaagtgacc
     8821 agttggttga cagggtatga agtgagtgac gttcttgctt gtctggatag ggatgttact
     8881 tacggagatt tttttcgaca agcaccatat tatgtccctg aacgaattgc aattacaggg
     8941 aagatttgcg gtgtccgtat tgaggaaatt gatgattctc tgatgcagga aatacgtcgc
     9001 ttggacaagc tagtggattg gcttgccaag gggaaaacat cccaacaagt tctagaaaag
     9061 tatgagaagc ataaatgaaa aattatcaag aatggtatcg aaatatcagc tccagactaa
     9121 ctagtcgtcc aattctttta ttcctattac gcagtttcaa tcgtttgatg acagtcgcca
     9181 tgcccctggt ctatctgatc ttgctagtca ccacttacct gcaactagga cttggtcagc
     9241 aagttggggt ttatgtgctt attcctgcat caggttttgt gaccttgtcc ctgtttcgta
     9301 agaaaatcaa tcacccgaga ccttatgaaa cttgggatat ctgtcccctt cttgataagg
     9361 atagttcggg acggtcgatg cccagtcgcc atgtcttttc ggcagctatc atctccatgg
     9421 cctgtctgca tgctagtcta cctattggct tagtatgctt gaacttttca gctttgctgg
     9481 ggttggtgag ggttttaggg ggtgttcatt atcccaagga tgtcttggtt ggctatgctt
     9541 gtggtctggt gtggggattc cttttcttct tattatgaca tgtaagttaa gctagtccta
     9601 caaccactta ctgcttcaaa ttgaccactt gcttttttgc ccttgtattc ctaacttagc
     9661 tttgatatag tagagtcaga aaggagatgt gatgaagtta cgaattgaga ttgacggcaa
     9721 tttagaggag actgaaattg tcatcaaggc agcggctttg acagatgaga ttgcggattt
     9781 acagcggctc ttgcaagaat ccaagtctcc taggttgatt ttttataagg ggacgggtga
     9841 atattatctg gacttgtcgg aaattctctt ctttgagaca gaaggtagca agatttatgc
     9901 ccatacccag aaaaatgcct acgaagttcg gctcaaactc tatgagttag aggctatcct
     9961 tcctcgctat tttagtcgag tatccaagtc aacgatcgct aatattcggc aaatctattc
    10021 ggtggacaag tccttttcag gaacgggcac catttccttt tatcaaaccc acaaggaggt
    10081 tcatgtgtca cggcattacc aatccctcct aaaagaaaat ctaagaaata tgaggtaaga
    10141 acatgaaaaa gaaagcattt ggtattgttt tattggtttt agcagcttgg atcttgctgc
    10201 aagggaattt tggaattcct tctctaggag ggcacatttg gcccttgatt ggtattgctt
    10261 tttttgccta ccaatcagtt ggagctttgt tgcgtcgcca cttaacttca gcctctttta
    10321 cagctctagt agccttaatg attgctaacc acttttatgg cattttaccg attccaaacc
    10381 agacactaat ctgggcagga gttctaatcg ttctcggagt aaatatgttg actcattcta
    10441 acagaacttg gaatgggaaa aaatggtggt acaatgggaa aaaaacagtc gtcacggata
    10501 aggaagtcgc ttttggtagc gggaccttct ataagcaaga tcaagatctc gtagatgacc
    10561 aagtggaagt cgcttttggg gatgctaaaa tctactatga taatgcagag atgctaggtg
    10621 attttgcaac tttaaatatt gaagtggcct tcggaaatgc aaccgtctat gttccacaac
    10681 actggcgtgt agatttgaaa gtagaaacct cctttggtgc agctaaggct gacgctcctg
    10741 tagccccaac cagcaaaacc ttgactatcc gtggagatgt ggcttttggg aagttggaaa
    10801 ttgtctacgt taaataaaaa aatcttcact tcaaccatca aaatagacgt actaagagta
    10861 ggaaattgat gccttgctct gatttcagtt ctatggttgt tagactttaa aaaatgaaat
    10921 gctgccttta aaagttgtat atttttcgat attttggctt ttacgtttga tgtatctatg
    10981 tactacagcg tagatgatgt agtgtcaaat gcttttaaaa aacgaatgat attggacagt
    11041 ttttttgcct ttaattgctc aggaaccatg aaagtcagta cttgggttta tgacaaggga
    11101 gaatggtatt atgtaagttc ttctggttcc atgattgcga atgattgggt caaagacaat
    11161 ggcaagtagt attatctggc ttcatcaggt aagatgcttc gcaataccta cacacctgat
    11221 ggttactacg ttggcaactt aggtgcctgg caataagaat aatactcttt gaaaatctct
    11281 tcaaaccacg ccagcttcat ccacaacctc aaaacagtgt tttgagctga cttcgtcagt
    11341 tctatctaca acctcaaagc agtgctttga gcagcctgcg gctagcttcc tagtttggtc
    11401 tttgattttc attgagtata agaagtcctt ttccttaaag gaagaggctt tttacttgct
    11461 tttctgctgc ttcctcattt gtcctgaagc cttttttgtg ttaaaatgaa tggtatgaaa
    11521 gctaaaaaaa tgtggatggc aggcttggct ctgctaggta tcggaagcct tgctcttgcc
    11581 acgaaaaaag ttgcagatga ccgtaagctc atgaagactc aggaagagtt gacagagatt
    11641 gtgcgagacc atttttccga catgggggaa attgcgaccc tctatgttca agtttacgaa
    11701 agcagtctgg agagcttggt tggtggcgtc atttttgagg atggtcgtca ttataccttt
    11761 gtctatgaaa atgaagacct agtctatgag gaggaagtct tatgatacag ccagcaagtt
    11821 tagaagaatt agcatcttta gtggaaaaag cgggcaagaa ggtcttcctt tttgtggcag
    11881 actggtgtgg cgattgtcgt tatatttatc ctgccttacc agagattgag gagaccaatc
    11941 cagagttcac ctttattcga atggaccgag atcagtatat ggatttggcc aaactctggg
    12001 atgtttacgg aattcctagc cttgttgttc tagaaaagga caaggaaatc ggtcgttttg
    12061 tcaatcgcga ccgtaaaagt aaggagcaaa ttaacgattt tttagcagga ttgaaatagg
    12121 agaaaaagga aacaatgatt tttacgtata acaaagaaca tgtcggtgat gtccttatgg
    12181 tcatcgtgaa aaatagcgga gatgccaaac tgaatgtgga acgcaaaggc aaggtagccc
    12241 gtgttttcct caaagaaaat ggggaaacag tagcttggaa catttttgag gtttcgagct
    12301 tgtttgaaat tgcagagcgc ggtcaagtct ttttatcaga tgagcaagtc gctcgtttga
    12361 accaagaatt acaggcggaa ggttttacag aagaaattgt taatgataag gaacctaagt
    12421 ttgttgttgg tgaaattgtc gagatggtag ctcatccaga cagtgaccac ctcaacatct
    12481 gccaagttgc agtcgcaagt gacaagatag tgcaaatcgt tgcaggagca cctaatgcgc
    12541 gtgttgggtt gaaaaccatt gtggctcttc ctggtgccat gatgccaaaa gggaatttca
    12601 ttttcccagg cgaacttcgt ggtgaaaaga gttttgggat gatgtgtagt cctcgtgaat
    12661 tgcatttgcc aaatgctccg caaaaacgtg gggtgcttga attatcagaa gaccaagttg
    12721 tcggaacacc gttcgaccca gcgaaacact ggactgccta ggaacttgtc agcatctgat
    12781 agaaggaaat aagatggcaa aaaatgtagt gattacggga gcaacctcag gaatcggtga
    12841 agcgattgcg cgtgcttatc tggagcaggg tgaggatgtc gttctaacag gacgacggat
    12901 agacagacta gaagctctta aggcggagtt tgcagaaact tttccaaatc aaacagtttg
    12961 gacttttctc ctagatgtca cggatatgac aatggtaaag actgtctgct ctgatatttt
    13021 agaaacgata ggtcagattg acatcctggt caataacgca ggactggctc ttggcttagc
    13081 tccctatcaa gactatgaag agttggatat gctgaccatg ttggatacca atgtcaaagg
    13141 tttgatggca gtcactcgct gtttcttgcc agcaatggta aaagccaatc aaggtcatat
    13201 tatcaatatg gggtcaaccg caggaattta tgcctatgct ggtgcagctg tttactcagc
    13261 caccaaggcg gcagttaaga ccttttcaga tggactgcga attgatacca tcgcaacgga
    13321 tatcaaggtg acgaccattc agcctggaat tgtcgaaaca gatttttcta cagtgcgttt
    13381 tcatggtgat aaagaacgag ctgcgtccgt ttaccaagga atagaagcct tgcaagctca
    13441 ggatattgca gacacagtag tctatgtgac cagtcagcct cgccgtgttc agattacaga
    13501 tatgaccatt atggccaatc aacaggcgac aggtttcatg gttcataaaa aataaaaaat
    13561 tttctcgaaa agttacaaat ttctgtaact ttttttgatt tcctacgaat agataagtag
    13621 gaggaagaaa atatgtataa taaagttatc atgattggac gtttaacgtc tacaccagaa
    13681 ttgcacaaaa ccaacaatga caagtcagta gcgcgagcaa ctatcgctgt caaccgtcgt
    13741 tacaaagacc aaaacggtga acgtgaagct gattttgtca atatggttct atggggcaga
    13801 ctagcagaaa ctttggcaag ctacgcaacc aaaggtagtc tcatttccgt tgatggagaa
    13861 ttgcgtaccc gtcgctttga gaaaaatggt cagatgaatt atgtaaccga agtccttgtg
    13921 acaggattcc aactcttgga aagtcgtgcc caacgtgcta tgcgtgaaaa taatgcaggc
    13981 caagatttgg cagatttggt cttggaagag gaagaattgc cattttaaga attaaaaagt
    14041 ctgagttggt ctcaggcttt ttatcttgag aaagtcagac ttttttcttg attatttctg
    14101 actaagtgat acaatagaac tatgaattag cactcaggta taaagagtgc taataatatc
    14161 tatctcatta tggaggaaat cagatgttga aaccattagg ggaccgtgtg gtcttaaaag
    14221 tagaagaaaa agaacaaact gttggaggct ttgttctcgc aggttcagcc caagaaaaaa
    14281 ccaaaacagc ccaagttgtg gctactggac aaggtgttcg taccttgaac ggtgacttgg
    14341 ttgctccaag tgttaaaact ggagatcgtg tcttagttga agcccacgca ggtcttgatg
    14401 tcaaagatgg cgatgaaaag tacatcatcg taggcgaagc taacattttg gcaatcattg
    14461 aggaatagaa ggagaaagta agtatgtcaa aagaaattaa attttcatca gatgcccgtt
    14521 cagccatggt tcgtggtgtc gatatccttg cagacactgt taaagtaacc ttgggaccaa
    14581 aaggtcgcaa tgtcgttctt gaaaagtcat tcggttcacc cttgattacc aatgacggtg
    14641 tgaccattgc caaagaaatc gaattggaag accattttga aaatatgggt gctaagttag
    14701 tatcagaagt agcttctaaa accaatgata tcgcaggtga cggaactacg actgcaacag
    14761 tcttgaccca agctatcgtc cgtgaaggaa tcaaaaacgt cacagcaggt gcaaatccaa
    14821 tcggtattcg tcgtgggatt gaaacagcag ttgccgcagc agttgaagct ttgaaaaaca
    14881 acgccatccc tgttgccaat aaagaagcta tcgctcaagt tgcagccgta tcttctcgtt
    14941 ctgaaaaagt tggtgagtac atctctgaag caatggaaaa agttggcaaa gacggtgtca
    15001 tcaccatcga agagtcacgt ggtatggaaa cagagcttga agtcgtagaa ggaatgcagt
    15061 ttgaccgtgg ttacctttca cagtacatgg tgactgatag cgaaaaaatg gtggctgacc
    15121 ttgaaaatcc gtacattttg attacagaca agaaaatttc caatatccaa gaaatcttgc
    15181 cacttttgga aagcattctc caaagcaatc gtccactctt gattattgcg gatgatgtgg
    15241 atggcgaggc tcttccaact cttgttttga acaagattcg tggaaccttc aacgtagtag
    15301 cagtcaaggc acctggtttt ggtgaccgtc gcaaagccat gcttgaagat atcgccatct
    15361 taacaggcgg aacagttatc acagaagacc ttggtcttga gttgaaagat gcgacaattg
    15421 aagctcttgg tcaagcagcg agagtgaccg tggacaaaga tagcacggtt attgtagaag
    15481 gtgcaggaaa tcctgaagcg atttctcacc gtgttgcggt tatcaagtct caaatcgaaa
    15541 ctacaacttc tgaatttgac cgtgaaaaat tgcaagaacg cttggccaaa ttgtcaggtg
    15601 gtgtagcggt tattaaggtc ggagccgcaa ctgaaactga gttgaaagaa atgaaactcc
    15661 gcattgaaga tgccctcaac gctactcgtg cagctgttga agaaggtatc gttgcaggtg
    15721 gtggaacagc tcttgccaat gtgattccag ctgttgctac cttggaattg acaggagatg
    15781 aagcaacagg acgtaatatt gttctccgtg ctttggaaga acccgttcgt caaattgctc
    15841 acaatgcagg atttgaagga tctatcgtta tcgatcgttt gaaaaatgct gagcttggta
    15901 taggattcaa cgcagcaact ggcgagtggg ttaacatgat tgatcaaggt atcattgatc
    15961 cagttaaagt gagtcgttca gccctacaaa atgcagcatc tgtagccagc ttgattttga
    16021 caacagaagc agtcgtagcc aataaaccag aaccagtagc cccagctcca gcaatggatc
    16081 caagtatgat gggcgggatg atgtaagctt tctatagaaa acaacttata aaaaacacaa
    16141 aaggagggaa tgactaaccc ttctttttat aatattcact tctaaattga tttgagctct
    16201 cctaacttat atgataaaat aagactagaa aaaggagaag aacatgatcg atgtagaaga
    16261 aattctgtgc aagatgaccc ccaatcagaa gattaattat gaccgtgtca tgcagaaaat
    16321 ggtacaagca tgggaaaaaa atgagtagcg gccaaccatt ctcgtgcatg tttgctgtgc
    16381 cccttgtagt acctatacac tagaatattt gaccaagtat gcagatgtga ccatctattt
    16441 tgccaattct aatatccatc ccaaggcaga ataccataag cgggtctatg tcaccaagaa
    16501 atttgttagt gattttaatg agcggacagg aaatacggtt cagtacctag aagctcccta
    16561 cgaacccaat taataccgaa aactagttag ggggctagag gaggagcccg aaggtggcga
    16621 ccgttgcaag gtttgttttg actaccgact ggataaaaca gcgcaagtgg ctatggactt
    16681 gggctttgac tactttggtt cagccttgac catcagtcct cataagaatt ctcaaactat
    16741 caatagcatc ggaatcgatg tgcaaaaaat ttacacgccc cactatcttc ccaacgattt
    16801 caagaaaaat caaggctaca aacgttcagt agagatgtgt gaggagtatg atatctatcg
    16861 tcaatgttat tgtggctgcg tctatgcagc ccaagcccag aatattgacc tggtttaagt
    16921 taagaaggac gccacagcat gcttgttgga taaggatgtt gagaaagact attctcatat
    16981 caaatttata gtagattgaa actagaatag tacaccttta cttctaaaac attgttagaa
    17041 atcgatttga ctgtccttat ttcattttac tatactgtta cgaaattaga tatataaatg
    17101 aaaacttgcc tcaaggtagg ttttttgcta gtagaaaagc gatagacaga aattgttgga
    17161 ctcttaaaaa aatgaagttt tgctcaaaag agctttttaa attaaaacga gcaattatca
    17221 gttcaaatct gtttcaaaag aagaacggat aaattaatcg atgctttgtt tttgagttag
    17281 gaagttaagc atgccaaact gagtcgtagg aataaatttc tttatagaaa tgtgttacaa
    17341 ctctgctata acggaatatt ccttttttgc atttatataa cttatttaac actatcttta
    17401 ttcttgtgat ataatgccaa att
//
LOCUS       DAVKWZ010000040        17169 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000040 DAVKWZ010000000
VERSION     DAVKWZ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17169
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.040"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            1..973
                     /locus_tag="VW854_001446"
                     /pseudo
     CDS             1..973
                     /locus_tag="VW854_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000530077.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            complement(1144..2706)
                     /gene="guaA"
                     /locus_tag="VW854_001447"
     CDS             complement(1144..2706)
                     /gene="guaA"
                     /locus_tag="VW854_001447"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEV0262667.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            2848..3546
                     /locus_tag="VW854_001448"
     CDS             2848..3546
                     /locus_tag="VW854_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV0262668.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKYGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(3589..4485)
                     /locus_tag="VW854_001449"
     CDS             complement(3589..4485)
                     /locus_tag="VW854_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV0262669.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAIFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(4494..5429)
                     /locus_tag="VW854_001450"
     CDS             complement(4494..5429)
                     /locus_tag="VW854_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEV0262670.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(5426..6151)
                     /locus_tag="VW854_001451"
     CDS             complement(5426..6151)
                     /locus_tag="VW854_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEV0262671.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDRASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLGKDRNNVELWFEEV"
     gene            complement(6235..6870)
                     /locus_tag="VW854_001452"
     CDS             complement(6235..6870)
                     /locus_tag="VW854_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEV0262672.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVACDYPLTEIKLLSILPVNEGEEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKALKDYLY"
     gene            complement(6872..7699)
                     /locus_tag="VW854_001453"
     CDS             complement(6872..7699)
                     /locus_tag="VW854_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV0262673.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALAHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(7721..8056)
                     /locus_tag="VW854_001454"
     CDS             complement(7721..8056)
                     /locus_tag="VW854_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HEV0262674.1"
                     /translation="MGNFISNQRIESMGDEENAKWTERGVLMDVTIKKKDGKTTIGTA
                     KAHPTWVNRTPKGTFSPEGYPLYHYQTYILEDFIEGGSHRDQLDEATKERIDTAYKEM
                     NEHVGLKWY"
     gene            complement(8057..8773)
                     /locus_tag="VW854_001455"
                     /pseudo
     CDS             complement(8057..8773)
                     /locus_tag="VW854_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
     gene            9286..9897
                     /locus_tag="VW854_001456"
     CDS             9286..9897
                     /locus_tag="VW854_001456"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV0262675.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            9942..10670
                     /gene="rlmB"
                     /locus_tag="VW854_001457"
     CDS             9942..10670
                     /gene="rlmB"
                     /locus_tag="VW854_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEV0262676.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKNLSEMTEGAVHQGFVLRVSEFAYSELDYILAKTRQEENSLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(10709..11620)
                     /gene="trxB"
                     /locus_tag="VW854_001458"
     CDS             complement(10709..11620)
                     /gene="trxB"
                     /locus_tag="VW854_001458"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEV0262677.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVRKIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDSHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(11686..11910)
                     /locus_tag="VW854_001459"
     CDS             complement(11686..11910)
                     /locus_tag="VW854_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEV0262678.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTAKARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(11988..12731)
                     /locus_tag="VW854_001460"
     CDS             complement(11988..12731)
                     /locus_tag="VW854_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV0262679.1"
                     /translation="MIKISNLSKSFSGQTVLDHLDLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(12731..13531)
                     /locus_tag="VW854_001461"
     CDS             complement(12731..13531)
                     /locus_tag="VW854_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV0262680.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(13689..14045)
                     /locus_tag="VW854_001462"
     CDS             complement(13689..14045)
                     /locus_tag="VW854_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEV0262681.1"
                     /translation="MLEFIEYLKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNREAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(14039..14569)
                     /locus_tag="VW854_001463"
     CDS             complement(14039..14569)
                     /locus_tag="VW854_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEV0262682.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGEPQDLSNLLLAPIGTNFEKRVWSYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDRNNRRRSTC"
     gene            complement(14569..15063)
                     /locus_tag="VW854_001464"
     CDS             complement(14569..15063)
                     /locus_tag="VW854_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0262683.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            15198..15644
                     /locus_tag="VW854_001465"
     CDS             15198..15644
                     /locus_tag="VW854_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEV0262684.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            15641..16288
                     /locus_tag="VW854_001466"
     CDS             15641..16288
                     /locus_tag="VW854_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEV0262685.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     NQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASVLQYIAIIYYM"
     gene            complement(16640..>17169)
                     /locus_tag="VW854_001467"
     CDS             complement(16640..>17169)
                     /locus_tag="VW854_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262686.1"
                     /translation="YVINTGFWNLLSNPSYLKSIQTSMSMGVTLISGFILYTQNMYSF
                     MNTANGVNEARASFIAYMSAPIILIITSSISLTILSSNKDIEDIKQLKTLGVSRLQLF
                     KIRIGEAIIHSVLILLVSVIFNLIILILVSIIGQFLGRSLVDISGFWQPSLIVISLLV
                     IFYSITKGFYLFQDR"
BASE COUNT         5219 a         3838 c         3011 g         5101 t
ORIGIN      
        1 aaaagttaat tgaaaagatt tctatgactg atattgccca tcagctggcc atttcaactt
       61 caactgttat tcgcaagctc aatgactttc actttgagca tgatttttct cggcttccaa
      121 agattatgtc ttgggatgag tatgccttca ctaagggaaa gatgagtttc attgcgcaag
      181 attttgataa tcttaatatt atcactgttc ttgaaggcag aacacaagct gtcatccgaa
      241 atcactttct tcgctacgat agagccgttc gttgtcaagt gaaaatcatt acgatggata
      301 tgtttagtcc ttactatgac ttggctaaac agcttcgctt tcaaatttct aggctcaggc
      361 tgaaacagtc tcccagactg tttcactccc gaatgctaaa atcgttcttg atcgctttca
      421 cattgtacaa catcttagcc gtgctatgag tcgtgtgcgt gttcaaatca tgaatcagtt
      481 tcatcgaaaa tcccatgaat acaaggctat caagcgctac tggaaactca ttcaacagga
      541 tagccgtaaa ctgagtgata agcgatttta tcgtcctact tttcgtatgc acttaaccaa
      601 taaagagatt ttaaacaagc ttttgagtta ttcggaagac ttgaaacacc actataaact
      661 ctatcagctc ttgctttttc atttccagaa taaggaacca gagaaatttt tcgggcttat
      721 tgaggacaat cttaagcagg ttcatcctat ttttcagact gtctttaaaa ccttcctcaa
      781 agataaagaa aagattgtca acgcccttca actacactat tctaatgcca aactggaagc
      841 gaccaataat ctcatcaaac ttatcaagcg caatgccttt ggttttcgaa actttgaaaa
      901 cttcaaaaaa cggattttca tcgctctgaa tatcaaaaaa gaaaggacaa aatttgtcct
      961 ttctcgagct tagctgactt caacccacta cagttgacaa agagccattt tttgtccaaa
     1021 tgtaaatcac ttggaactat tattcaattt attcttttta gtaaatgttc ccgttttaat
     1081 cccagttaca gtttttaatc ctctgtaacc gttgatttta caaagctttt aatttttttg
     1141 cgattactcc cactcaactg ttgcaggtgg tttacttgta atatcgtaga cgatacggtt
     1201 aacatgatcc acttcattta cgatacgtac tgagattttt tgaagtactt cccatggaat
     1261 tttggcaaaa tcagcagtca taccatcgat agaagtgata gcacggattg caatcgtgta
     1321 gtcatacgta cgaccgtcac ccataacacc gactgaacga acgcctgtgt taacagtgaa
     1381 gtattgccaa atatcgcggt caagtccagc tttagcgatt tcttcacgaa gaatagcgtc
     1441 tgattcacga acggtttcaa gtttctcttc agtgatttca cccatgacac ggatagcaag
     1501 tcctggtcct gggaatggtt ggcgccatac gatatggtct ggcataccaa gctctgtacc
     1561 aagagcacga acttcatcct tgtaaagagt attgagtggt tcaatcaatt caaactgcat
     1621 atcttctgga agaccaccca cgttgtggtg tgacttgata gtttgagctg tatccgtacc
     1681 agactcgata acatctgtat ataaagtacc ttgagcaagg aatttcacat ctttgagctt
     1741 gcttgcttcg tcatcgaata catagacaaa ctcgttaccg atgattttac gtttttgttc
     1801 aggatcagaa acgccagcaa gtttgtcaag gaaacgttta gcagcgtctg ctttgacgat
     1861 attcaaacca aacttaccac cgagcatgtc cataacttga tcagcttcgc ctttacgaag
     1921 aagaccgtgg tctacgaaga tacagatcaa ttgatcgcca atcgcttttt ggagaagaac
     1981 cccaacgaca gatgagtcaa caccacctga tagaccaaga aggacacgtt tatcaccgac
     2041 ggtttcacga atttttttga tctgcatgtc aatgaaatta tccattgacc agtcaccttt
     2101 agccttacaa atgttaaggg caaagttacg aaggatatca tttccgtata cagaatgacg
     2161 aacttctggg tggaattgga taccgtaaat gtgtttatct gggttttcga tggctgcgta
     2221 tgggcagtca gctgatgtac ctgtacgaac aaagtcagca ggaatctcag taaccgcatc
     2281 accatggctc atcaaaacag tctgttcatc aggtgttgat tcaaaaagcg ctgatggtgt
     2341 gtgagttagg gttgattgac cgtattcacg atttccagca tcacctgcag gaacaacttt
     2401 tcctccaagt ttatgggtca ataactgcat accataacag attcccaaaa ttggaattcc
     2461 gagttcgaag atttctgggt caatatcaaa tgaaccatct tcatatacag aatttggacc
     2521 acctgataga ataattccta caggattgac ttcacgaact tcagcagctg aaattttatg
     2581 gctttttagt tctgaaaaaa caccaatctc acggatacgg cgtgaaatca gctggttgta
     2641 ctggctacca tagtccaata cgatgatttt ttctacatct tgcaaatcag ttgaaatgtt
     2701 gctcatcttt ttcctttctc aaaagaaata tcttttccca atattctatc acaaataagg
     2761 gcgaattacc aactaaacaa ggcgaagttt gactttttct agtttattgg ggtacaatag
     2821 agaaaagata gaaatgaagt gaaaaatatg ctacctgctt atatgaaaat ccatgatcag
     2881 ataaaaaagg atattgacga gcaccgttgg gctattggtg agaggcttcc cagtgaaaga
     2941 gatttagctg agcagtttgc ggtcagtcgc atgaccctcc gccaagccgt atctctatta
     3001 gtcgaagaag gcgtcttaga gcgccgtgta ggaagcggca cctttgtttc cagtactcga
     3061 gtacaagaaa agatgcgagg gacaaccagt tttactgaaa ttgtcaaatc ccaaggtaaa
     3121 gttccctcta gccagctcat ttcctacaaa aaaaccattc ccaatgagca agaagttgcc
     3181 aagctaggaa tttttccaac ggacaatatt atccgaatgg agcgggtccg ctatgccgac
     3241 caagttcccc tagtttatga agttgcttct attcctgaaa aattcattaa ggactttaaa
     3301 aaagaagaaa tcaccagtca tttcttccaa accttgcaaa aatatggcta tcgtatcggc
     3361 aaatctcaac agaccatcta tgctcgcctt gctaaagaaa agattgccca ctatttggaa
     3421 gttgaaaaag gacatgctat tcttggattg acccaggttt cctacctaga agatggtact
     3481 gcttttgaat acgtaaaaag tcaatatgta ggcgaacgct ttgaatttta tcttgaaaat
     3541 aattagatta ccacgcaaaa actctctgtc acggacaaag agtctttttt atttttctaa
     3601 gaataaatct ttcaaagaaa tcaaagttac agcaaacaat atcattgcca taccaagaaa
     3661 atcaatggga taaaattgtt cattcattat taagaaggca aagaaaatcg ccgatattgg
     3721 ctcaattgaa gccaacaagc ttgacttgac cggtcctatc agactggctc ctttaaggaa
     3781 agctgtatag gcaaagacag tcccgataag gataatgcct gcaaacgcaa ggagaaaatc
     3841 aagactagtc gggatatcgg cctgtagaac ccctgtaaaa ggaagggcga ccaaacctgc
     3901 tatgaccatt cccacaccaa tgaccaagct gctcccccac tttttaatca aggctatggg
     3961 taaaatgata tacagagcat aagtcaaggc agaaaagaga ccccagaaca gaccagcagg
     4021 tgtcatggat aactggtcca actgcccatg tgttgcgatc aggaaggttc ctccgatggc
     4081 gaatatgatg gaaactatct ctcccagtgt cggtgccacc ctatccttga tacagctata
     4141 aattaaaatt ccgacaggac aaacatactg aagcaccgtc gctgttcccg cattggtctc
     4201 ctgaatagca gacagatagg cgaattggtt gagaaaaaga ccaatcagag caaaaataag
     4261 aagagacagc aaactctttc tatcctttaa aaagaccagt attttatcct ttgcagtagc
     4321 ataagccaag agcatgagaa ttccaccagc gattaaaaga cgcaagttgg tcaagaccag
     4381 agccgaaatt ccgtgtgcca ttaggtattg gccactcgtt cctgacaacc cccaagcaat
     4441 accagccaca actgttagta aagtcccttt taaactattt gacatgtctc tccttactct
     4501 tctttgcgaa tcaagttcat cacttgattg cggtctaata tccattgagc tctctcgtcc
     4561 ttttcatagg tacgattcga taggaaaata gtggcttctt gcttctgacg attccacatg
     4621 ataaaggtac ctgtatagcc cgtatggtct agccaatctc cttccaaatt ccatgctaaa
     4681 gaacgttcct tgtcatccaa aggagaaaaa ttttgattta agtctcttgc aaaatcatct
     4741 gctaaatagt gttctaaaaa gatttgtaaa tcctttatag tcgaaaataa accagcactc
     4801 ccagcatgtc tacccaggag acgagccttg ggatcatgca ctatgcctgc ctctacacct
     4861 ctaactgttg gaacagcaag ctcaactggc ccaaacttag tttccgtcat tccccaaggt
     4921 ttccagactt gatcctttaa aatcacatcc aaatcttgat taaaaattct ttccaaaata
     4981 aagcccaaca gcaaaaaatg gacatccgaa taaagaaagg ctggctgact tcgtctgttg
     5041 agatgaaaca tcgcttcctt taattcaggg gctgttaaaa gatcacgatt aggaataaaa
     5101 ggatcaaggt ctgttgcatg agtcaagagc tggcgaatag tgatgtctgg ataatcactc
     5161 tcaggtaaaa aatctattac cagtctatca atatctaatt gacctatttc ccacaagaag
     5221 gtacaaactg tgccaacccc aacaacctta ctgacactag ctaggtcata aactagtcct
     5281 gcctcagtct gcaagccatg ctctgggtca ctctggccta aatagaactc tgtccattga
     5341 ttgtccttaa aatacgcaaa agaggctccg ggataaattc ctgcctcgat ttgttcttct
     5401 atttttttaa taatcttggt ccacttcata cttcttcaaa ccacaattca acattatttc
     5461 tatctttacc aaggaagaat ttttcagact taggaataaa atattcagta gactcaaact
     5521 tctggcgaag acttgctatg tctaattcat tgaccaagaa cttgagcata gataagtccc
     5581 aggtaaccgt attgtccaca gtcaaatcct gcccctgagc tggaataaaa tcaagggatg
     5641 ccccaatttc agatgattcc aagaaacttt cgatatcagt tgggagatgt aattccatag
     5701 aaatctcaaa tttacttaaa gaaattgatg ccaaatctgt ttgaaattca ggcttttctc
     5761 ctacttctac tagacttgct ctgtcatctt ccgcatgaat caaaatcaaa tcatcttctg
     5821 gtgagaaaat ttcaaaagcg tagccatttt gacctttata taatcgatga atcgaatctg
     5881 ttttagataa gattccttca atttctaagg gattttccac cttgacaatc aatctagcta
     5941 gtttttttct tccctctacc ttacgagtac gcatactggg agcttcttct aaaaccagct
     6001 tttcaagacc cgtttggtca cctagtgaca gaaaggccga ttcttctaac aaggccttca
     6061 ttccaagggt ttcaatataa aatgtttcat ttaattttct attattagct tttaaagtag
     6121 gaataatccg tacaatctga tttacattca taaattcctc cgtcactttt tattttaaag
     6181 gattttagaa ttttttacaa gatattatgc tcaaatttaa cattatttag ctatttagta
     6241 aagatagtct ttcaaggctt ttgacaaagc ctgataacca gcaatactga ggtgcagtcc
     6301 atcagttgta tattcttttt tgagttggcc tgcttggtct gtcaaacaat caaatactgg
     6361 cacaaattcc acctgcatat aggcagatgc aagctcttga taggcttgat tccagttctg
     6421 aattttttca ttcgagcgga tatagactgc ctgctggtac tcctctccct cattgacagg
     6481 caaaatggaa agcaatttaa tctctgtcaa tggataatcg caagcaacgg attgaatgat
     6541 agcttcgaga ttattgagag cctcattcac aggaacatcc tttccgatat catttgtccc
     6601 aatcagaaga aaaattttat ctactgctcc accatataga tgagcatcaa ggttctctag
     6661 taacagtcct gtctgatagc cacgaattcc tcgattgaca atcgtctttg aagtcccaaa
     6721 tagctcctgt agaggataat actcgacaat ggaatcccca ataaaaagaa tgtttggttc
     6781 tacaacagaa atgtgattta ggtgacgata cttggtttga attttttctt gttcctttag
     6841 gagccaattt tctaataact gtactgccac ttcattctcc tttttctaac cagttttcca
     6901 agacttgata aactcctcct tggctgttgg ctggtgctag agcagtcgcc acagctttgg
     6961 ctttctcatc agcattttcc atcgcatagg caattccagc catttcaagc atttcaacat
     7021 cattttcact atcaccaaaa gccatgattt cttgggattt caagtcccag cgcttgagta
     7081 attcctccaa gccccatgct ttatgaatcc cagcttggag gatatcaatg caaccatagc
     7141 cactggatac agctcggaca cggccatcaa agagagcatt gatttcttcc aaaaccgaac
     7201 tcaaacgttc ctcaccaaca accatgctca tcttgagcac accaccaaag aggtcagatg
     7261 ttaattcatc cacaaattgc atccgttggt agaatttttc aatcatttct ggagtcataa
     7321 aactttcaag atctgtaaaa atcgtacctt ccttgacaaa atcacccttc atccccgtta
     7381 caacaaactg gtcctgacac gctcgaccct tgaaatgagc caaagccttg ttgacaatgg
     7441 cgtcatccca tgtctgagcc tgaatcaatt cattgttttc aaaaatacga gcgccattag
     7501 caacaaccag aaccactcga tccaccaagg gactcagtag ttgtctcatg cggtgaattt
     7561 cattgcccgt cgcaatgaca aaacgaatgc ccctttgatc caactgatct aaaatctttt
     7621 ccaatcgtgg gagatcaagc tgacctctag catccagcaa ggtcccatcc atatctgtcg
     7681 caattacctt aatcatcatt tactttcctc tggattcaag ctaataccac ttcaatccca
     7741 catgttcatt catttcttta taggctgtat caattcgttc cttagtcgct tcatctaact
     7801 ggtcacgatg actgccaccc tctataaaat cttccaaaat ataagtttgg taatgataca
     7861 agggatatcc ttctggtgaa aaggttccct ttggtgttcg attgacccaa gtaggatgag
     7921 ctttagctgt tccgatagtt gtttttccat ccttcttctt gatggtgaca tccatgagaa
     7981 caccacgttc agtccactta gcattctctt catctcccat agattcaatc cgttgattgg
     8041 aaatgaagtt ccccatttaa tagataatga gtttcttatc tccatctttt tcaaccgttt
     8101 cagatggttc aacaacgtga ggatgccctc caaagataat atccgctccc caatcgatca
     8161 tcttgtgata aagagctttt tgttcttcag ttggttccaa tcgatactca acacccatct
     8221 gaagcatgat aatggtgata tctgcttcct tctctgcccg ttcaatttca gccttcatct
     8281 tatcttcgtt taaatctgaa agataacgat tatagtcttc ctgagaaata tactgctcaa
     8341 ttccattgaa accataggaa taggctaaca atgcaacctt gatagcattc acttccttaa
     8401 tgaccagcgg agcctgaaca cgtggttcgt gcgtataaac tccgattgga gtgattccag
     8461 ctttctcaat aatatcggcc gttgaaataa ctccctcaat ttgcgaatcc aaaatatgat
     8521 tatgagctaa atccagcaca tgataacctg cctccttaat agcatccata acttcagcag
     8581 gagcattaaa gagaagataa cccgctaaat aatgatcctt attaatggtt ccttcaaaat
     8641 caccaatagc taaatctgct tgcttgagcc aaggagtcac atactaaaaa ttttcatgaa
     8701 agtcataggt accgtcttct tttttagctg aaaagaaaag tccatcgtgg tagagcaaat
     8761 ccccgtgggc cataattctg gcagtttttt cctcttccta ctcctgagac ctttgcttag
     8821 tcccctcttg agaaatagta tctcgttttt gactagtcaa gggattcctt ggaagctttc
     8881 aaacaacaag accaagccca ttgataaacc aactgctagc aagagtatcg ccacaaatcc
     8941 cttattgctc cacttgcgat aactcctaaa aagtttacca agcccttcat aaaacgaaaa
     9001 gctaaaccac cctgatttcg attttgtctt ctttgtatct tcattctccc tactttctta
     9061 tgcaagcctt ttctttttat tatatcacag ataagtattt ctttcacaat tgaattgaac
     9121 ttcccatcta ttttctataa atcctaaatg ccataatgct ttcaattctt gtcattttgt
     9181 gatatcatgt agaggaaatg aactaatcca cagtggctta ttccaagtat accacttggg
     9241 ctttggcagt agctaactgc actaaacata atataaggag agaaaatgtc tgcaatagaa
     9301 cgtattacaa aagctgctca cttaattgat atgaacgata ttatccgtga agggaatcct
     9361 actctacgcg cgattgctga ggaagtcact ttccccctat ctgaccagga aatcatccta
     9421 ggcgaaaaga tgatgcaatt ccttaaacat tcccaagatc ctgtcatggc tgaaaaaatg
     9481 ggactccgcg gtggtgttgg actggctgct ccccagttag atatctcaaa acgcattatc
     9541 gctgttttgg tacctaatat tgttgaagaa ggcgaaactc cacaggaagc ctacgatttg
     9601 gaagccatta tgtacaatcc aaaaatcgtc tctcactctg ttcaagatgc tgctcttggc
     9661 gaaggagaag gttgcctgtc tgttgaccgt aacgtgcctg gctatgttgt tcgccatgcc
     9721 cgcgttactg ttgactactt tgacaaagat ggagaaaaac accgtatcaa actcaaaggc
     9781 tacaactcca ttgttgttca gcatgaaatt gaccacatta acggtatcat gttttacgat
     9841 cgcatcaatg aaaaagaccc atttgcagtt aaagatggtt tactgattct tgaataaaga
     9901 aaatcccgtt gcaagacggg gttttgtgtt ataatagagg catgaaaaca aatgatattg
     9961 tctatggtgt ccacgccgtt accgaagccc tccttgcaaa tacaggaaac aaactctacc
    10021 tccaagaaga tctccgaggt aagaatgttg agaaagtcaa ggaactagct acagaaaaga
    10081 aggtgtccat ttcttggaca tcaaaaaaaa atctctctga gatgactgaa ggtgctgttc
    10141 atcaaggttt tgttctacga gtgtctgaat ttgcctatag cgagctagat tacatccttg
    10201 caaaaacacg ccaagaagaa aattcacttc tattgattct agatggtcta accgatcccc
    10261 ataatctggg ttctatcttg cgaacagccg atgcgaccaa tgtttcaggt gtcatcattc
    10321 ccaagcaccg tgctgtcgga gtaactcctg tcgttgccaa aacagccaca ggtgctattg
    10381 aacacgttcc aattgcccga gtaaccaacc tcagtcaaac cttagataaa ctcaaggatg
    10441 aaggtttctg gacctttgga acggatatga acggtactcc ttgccacaag tggaatacaa
    10501 aagggaaaat cgccctcatc attggaaatg aaggaaaagg tatctctagc aacataaaaa
    10561 aacaggtcga tgaaatgatt accattccga tgaatggaca tgttcaaagc cttaatgcca
    10621 gtgttgctgc ggccattctc atgtacgaag ttttccgaaa tagactataa aaaagtttcc
    10681 agtcatctga ttggaaactt ttttatgatt aactatgttc tgtaatgaat ttataggctt
    10741 cttgaccagc gatagctcca tctccaactg ctgttgttac ttggcgaagg tctttcaagc
    10801 gaacatctcc aactgcaaag ataccgtcga ctgcagtctt catgtggcta tctgtcacaa
    10861 tccagcctgc ctgatcttgg atattcaatt ctttaacaaa atcgctaaga gggtccaaac
    10921 caacatagat aaagacacca ccgaaggctt gctctgtcac ttgacctgtt ttcacatttt
    10981 caaaaacgac agattctact ctgttttcac ccttgatttt ccttactaca gaatcccata
    11041 taaagctgat tttctcattg gcaaaggcgc gatcttgtaa aaccttttgg gcacgaagtt
    11101 ggtcacgacg gtgaacaatg gtaacagtct tagcaaaacg agtcaagaag agggcttctt
    11161 caacagctga atctccacca ccaactacca ataaatcttg gtcacggaag aaagcaccat
    11221 cacacacagc acagtaagaa acaccacgac tgttcagttc ttcttctcca ggcactccca
    11281 aaggacggtg tttagaacca gttgctacga taactgtacg tgtttcatat gtttggtcat
    11341 cagtcatcac tttcttaaaa tcaccatggt cttcgacatt ttcaacataa ccataaatgt
    11401 gctcaacacc aagattttca agtggttcaa acatcttttc agccaattca ggtccactaa
    11461 tattagcgta tcctgggtaa ttttcgatat cagatgtatt attcatctga ccacctggca
    11521 gaccaccttc aatcaaagct acttttagat tgcttcgagc agcatacaag gccgcagtca
    11581 tccctgcagg tccagcaccg ataataatag tatcgtacat atagattcct tctttcttgt
    11641 tgtaactatc tttattctaa ctctttcttg tcaatcacgc aaggattatg ccttgaaaac
    11701 aagaattaaa ctcataaccg caaaggataa tactgggaca accaagactc caatcataat
    11761 catatcatag agatgggctt ggcgagcctt ggctgtctta tcaactcccg acatggctct
    11821 cagaccaatc caaatcccta aaaaaatcag aacaaggatg gtggtcaata tcaaactctc
    11881 gaaatataaa gaaaatagtt gcagtagcat gatttctctc atttctatct tttttaaaga
    11941 gtaaactcag ctagtccaac taactgagtt ttcctttatc tattatatca aatataagtc
    12001 cgtttgtaac tagcgaagaa ttcttttgtc cgctcttctt taggggtgtg gataatctca
    12061 tccggtgttc cagactcgat gattttcccc ttatctaaga agagaatctt atcagccact
    12121 tgggctacaa aggacatgtc atgactaacc aaaatcatgg tttgacctga cttagcagca
    12181 tctgcaatag acttttctac ttcaccaacc aattctgggt caagggctga agttggttcg
    12241 tctaagagca aaacatctgg tttcatagca agcgcacgcg ctagggcaac ccgttgcttc
    12301 tgtccacctg ataaatggcg aggataatgg ttttcacggt ccgaaagccc aaccttagcc
    12361 aactcttcct tggcaatctt agtcgcttct tggtcagata atttcttgac aacaaccaag
    12421 ccttctttca cattatcaag tgctgttcgg cgttcaaaca aattaaactg ttggaaaacc
    12481 atagacaact tacgacgtag ggcaaggatt tcttcttgag tgattttaga aaaatcaact
    12541 gaaaaaccat caatctgaat agagccactg tcaggtgttt caagataatt gagactgcga
    12601 agaaaggttg attttccagc tcctgaagaa ccaatcaagg ctacaacttc ccctttttga
    12661 atatccaagt ccagatgatc caagacagtc tgtcctgaaa aggatttgct taaatttgaa
    12721 atcttaatca ttaacgaagg tctcctttca catctgtttg cactgtatca ggtgcagaaa
    12781 tagccatttt tctctcgatg aaacgaccga ggctttcaat tccgatattg accacccaat
    12841 aaacaagggc aacggagatg aagcgttcaa aatagcgata atcagctcca cctagaatct
    12901 gagcttgggc aaagacttcc acaacacccg cactaaaagc tagagatgtt cccttggtca
    12961 aaccgatgag ggaattaatc aaggttggag tagctaccac cgctgcatta ggaataatca
    13021 ctcgtcgata aacttgcgct cgggtcatac ccagactgcg tgccgcctca atctcaccag
    13081 gattaactga gagaatggct gcacgaatgg tttcactagc ataagctgcc tcattaaagg
    13141 caaaggcgac aatcgcaaaa gctgcagctg gaatcgcatt gatattgaga ccagttcccc
    13201 attgctgatt gagggctttc aaagccaaag ggattccgta gtaggtcaac atgagttgca
    13261 ccaaaatcgg tgtccctttt aagaaactaa caaagaaggc ctgcaaggga tataaaatct
    13321 tgacacgatt gatcttcaca atggcaaaaa gaagcgccaa aaccaagcca aaaagggcac
    13381 caccaattgt caacataatt gttgttggaa gttgttggac aattctaggg attccatcaa
    13441 agaccgaacg taggctaaac agcttgccat ccggaatcaa ttgcatcaag ttttggtacc
    13501 aatctgatgc taaaatcgtt gtaacattca taaaaacatc ctcttctgat aatgattcat
    13561 tctaccatgc ttctgccgtc aatgaaatta tttgctacgg tcagatacag actttgtcta
    13621 cctactagtt tatcatactt tgaaacaggg aagttatata ttttatctat caaagagata
    13681 gataaaaact atttcagtcc cagttcttca taagattttc gataaccgat ttgcttaaca
    13741 gttccatttt ctactaaaat gggccgtttt aacaacatac cgtcacttgc tagcaactca
    13801 gccgcttctc ggtttgacaa acttcctacc ttatctttta gccctaattc acggtatttg
    13861 atcccactgg tgttgaaaaa ttgcttcaat tcaaatcctg aggtttctag ccaattcaaa
    13921 atgacttctt ggctaggtgt ttcttccacg atatggacgg ctttatagtc cacacctaat
    13981 tgatttaatt cttgttttgc ttttttacaa gttgaacatt tgaggtattc gataaattct
    14041 aacatgtgct tctccttcta ttgtttctat ctttaaaatc tactccttca tgctccaaga
    14101 gccaagcttt cttttccact cctgcagcat agcctgtcag acgcttgcct gctcccaaca
    14161 cacgatgaca aggtactagg atagaccaag gattgcgtcc cactgctcca ccaattgctt
    14221 gagcagaagc cacttgcagg tcttgagcaa tttgtccata ggtcactgtc tgaccataag
    14281 gaattccctg taaataggac caaactctct tttcaaaatt cgttccaatt ggcgccaaga
    14341 gcaagttgga taaatcctga ggctcgcctt taaagtaatc atctaagcaa gcaataactg
    14401 gatctaaaat aggatgacta acaacttctt ctatcgtttc atctcctagt cccctctcaa
    14461 aatgcttctg ctcctgaacc caaattccat acaaataatg gtcatcagct acaagtgata
    14521 gaataccaat tggtgaggag tagagtattt ttacatactt cttttgcatt atttctttaa
    14581 tttttgatag gccaagcgtt cgccatctac tggcacctta ccaacttgtt taaaaccaag
    14641 tttttcaaaa atatgttgca taaccttgtt ttcagcatgc gtatctgagc gaaaatcaag
    14701 ataatcaaaa ccttcaatca agccctctaa gaaggtttga gcaactcctt ttccctgcac
    14761 atctgctgcc acagcaatac ggtgaaagac tagatactct gactctccag cttgccagtt
    14821 tccctcataa atagcttcat aggcctcctc tggactcttg gtcacagcag cataggctag
    14881 cagttctccc tcttccaagg ctacgtaggc ttgccctgag ataatatcat cgataataac
    14941 atctgcattt ggatagccat tttgccactg gtcactacca gcatctgcta aacatttttt
    15001 ggcatcctcc atcacctgca tgatggcatc tacttcattt ggaaaagcta aacgaatttc
    15061 catctttttt cctcatttct gactagtttc tcccatcata gcataattta agtatttcta
    15121 caagcagtta aaacttgact ttgtgttttt gttattttat aatctagtta tcaaaactat
    15181 ataaaaagga gttgaaaatg aaaactacct tttcctaccc aaaatgggca gaaattccaa
    15241 acattgacct ctatctggac caggttttgc tctatgtcaa tcaggtctgc gcccctatct
    15301 ctcctaataa agacaagggc ctaacagcat ctatggtcaa caattatgtg aaaaatggtt
    15361 acctgacaaa gcctgacaaa aaaaaatacc aacgccaaca gattgcccgt ttgattgcta
    15421 tcacaaccct caagtctgta ttttctattc aagaaatagc ccagacactt aatactctac
    15481 aaactcaagc aagttcagac caactctacg atgcttttgt ggactacatg aaccaaggaa
    15541 ttgacccagc taaccctatt atccaaacca gctgccaaac cgttaaactc tatcatcaaa
    15601 ctctagactt aatcgatcat actcaagagg aggtaatcca atgaacacta gtcttaaact
    15661 cagcaaacaa ctcagttttg gagaggagat tgctaatagc gtgacccatg ctgtgggtgc
    15721 agtcatcatg cttatcttgc tgcctatttc atccatctat agttatgaag cacacggatt
    15781 tttatcctct atcggcgttt ccattttcgt catcagtctc tttctcatgt tcctatcatc
    15841 caccatttat cactctatgg cctatggttc gacccacaaa tatgttttgc gaatcattga
    15901 ccattctatg atttacgttg ccattgccgg ctcatacacg cccgttgtct tgaccttgat
    15961 gaataactgg tttggctatc tgattattgt catccaatgg ggaacgacca tctttggtat
    16021 tctctataaa atctttgcta aaaaggtcaa tgagaaattt agccttgctc tttacctgat
    16081 tatgggctgg ttggttctgg ctatcattcc tgccattatc aatcaaacga cacccgtttt
    16141 ctggagtctc atggtaactg gcggactctg ttatacagtt ggagctggat tttatgccaa
    16201 gaaaaaacct tatttccaca tgatttggca tctctttatc ctagctgcgt ccgtactcca
    16261 atatattgct attatttatt acatgtaaaa ctctcagtag gcagagcctg cttagagttt
    16321 tacaaacgaa tttcccaaag aggaagcaat atgaaagcag tcattgcttg tgttcataaa
    16381 attctcagaa tcatttacaa actactctcc accaagtaga cttatcaaaa agaaaaggcg
    16441 ctaggactga agaaacagtt ctaacgccca aattaaaaaa atttcaacta cagtatagca
    16501 caggaaatga ttttttgcgc ttttttactg ttttttcaaa taaaaaaaga caaagtttta
    16561 tccttgtctt ttagttttag tattgctttg gtttcaatgt caataagtgt gttctttttg
    16621 gttcagtaac tattccaatt tatctatctt gaaataaata aaatcccttt gtaatactat
    16681 aaaaaataac taacaaagaa ataactataa gactcggttg ccagaaacct gatatatcta
    16741 ctaaactacg acctaaaaat tggccaataa tgctaactaa aattaaaata attaaattga
    16801 aaattactga cactaataaa attaacaccg aatgtattat tgcctcgcct atacgtattt
    16861 taaaaagttg taatcttgac actcccagtg tctttaactg tttaatgtcc tcaatatctt
    16921 tattagaaga taaaattgta agagaaatag aactagtaat aatcagaata attggtgcag
    16981 acatataagc aataaaggaa gctcttgctt cgtttactcc gtttgctgta ttcatgaatg
    17041 aatacatatt ttgggtgtaa agaataaacc cagaaatgag agttactccc atgctcattg
    17101 aagtttgtat acttttcaaa tagctaggat tggatagtag attccaaaag cctgtattaa
    17161 ttacataat
//
LOCUS       DAVKWZ010000041        16756 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000041 DAVKWZ010000000
VERSION     DAVKWZ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16756
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.041"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            790..1011
                     /locus_tag="VW854_001468"
     CDS             790..1011
                     /locus_tag="VW854_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0262687.1"
                     /translation="MYRRLRDLREDHDLTQKQIAKILSFTNSAYAKIERGEHTLTADV
                     LVTLSNFYDVSTDYLLELTDFPDKIRFRK"
     gene            1528..1767
                     /locus_tag="VW854_001469"
                     /pseudo
     CDS             1528..1767
                     /locus_tag="VW854_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            1911..3140
                     /locus_tag="VW854_001470"
     CDS             1911..3140
                     /locus_tag="VW854_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV0262688.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            3160..5856
                     /gene="ppc"
                     /locus_tag="VW854_001471"
     CDS             3160..5856
                     /gene="ppc"
                     /locus_tag="VW854_001471"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEV0262689.1"
                     /translation="MSLQKLENSSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    5939..6156
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6269..7273
                     /locus_tag="VW854_001472"
     CDS             6269..7273
                     /locus_tag="VW854_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV0262690.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNITGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            7342..8208
                     /locus_tag="VW854_001473"
     CDS             7342..8208
                     /locus_tag="VW854_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0262691.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            8205..8963
                     /locus_tag="VW854_001474"
     CDS             8205..8963
                     /locus_tag="VW854_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0262692.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEELTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            9053..10813
                     /gene="dnaG"
                     /locus_tag="VW854_001475"
     CDS             9053..10813
                     /gene="dnaG"
                     /locus_tag="VW854_001475"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEV0262693.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVTFMEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKTSEIYLMEGFMDVIAAYRAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAAILKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYAQIEQIVNESRQVQRQNRMEGISRPTPITMPIT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            10816..11925
                     /gene="rpoD"
                     /locus_tag="VW854_001476"
     CDS             10816..11925
                     /gene="rpoD"
                     /locus_tag="VW854_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEV0262694.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARILSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            11939..12268
                     /locus_tag="VW854_001477"
     CDS             11939..12268
                     /locus_tag="VW854_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV0262695.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            12383..13426
                     /gene="cpoA"
                     /locus_tag="VW854_001478"
     CDS             12383..13426
                     /gene="cpoA"
                     /locus_tag="VW854_001478"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEV0262696.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAVLKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            13442..14767
                     /locus_tag="VW854_001479"
     CDS             13442..14767
                     /locus_tag="VW854_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV0262697.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGILIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGALYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFRTVLYLPQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            14798..14974
                     /locus_tag="VW854_001480"
     CDS             14798..14974
                     /locus_tag="VW854_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV0262698.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            15033..15167
                     /locus_tag="VW854_001481"
     CDS             15033..15167
                     /locus_tag="VW854_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEV0262699.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            15231..16541
                     /gene="obgE"
                     /locus_tag="VW854_001482"
     CDS             15231..16541
                     /gene="obgE"
                     /locus_tag="VW854_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEV0262700.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLAENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            16551..16709
                     /locus_tag="VW854_001483"
     CDS             16551..16709
                     /locus_tag="VW854_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262701.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
BASE COUNT         5162 a         2915 c         3687 g         4992 t
ORIGIN      
        1 ataagatttg attccatttt agcaaaggcc ttacctaata ttttgctgat acttattgtt
       61 ttaatcacgt ttcagagtga ttggaatata tttatacctg caacttatta ttatacagaa
      121 attcagtcaa ctacaaactt aggtattaaa ttattagttt gtatctggat tatgggattt
      181 gaattttccc ttcttccaaa actaataaaa ttgaaagaac aaaatctagt ttaaatgttg
      241 ctcgaaaatt aaataattga atacgattaa caatgaaaca atacaacgtg agtcatgact
      301 tcaaagtaat tgattttatg attctggttt cttacgctta attaagcaag ttgtattcac
      361 tttatcaaat tgttattact ctaaaaagcg tctaacagct atagagtgtc tgtttgcttc
      421 tctttacttt tattttaaat aattcattaa ccttccgtaa gtgcccaata ataaggtgtg
      481 ttgaagaagt ctgagataaa atagttctca gtcacaaaaa aagctataga ttcccattgg
      541 tgaacagctc cctgtcaagt agacagtaaa ataatataaa atcttaagcg acctgattcc
      601 tgaattccag tggagtcagg ttgtttttta accgtctttc ttaatattgt ctcacctata
      661 atagatatgc ttatgagata tgcttatgag atatgtttaa taaattaatt ttagttcaaa
      721 atgtccagtt aggacacttt gaatgatttc ttagttttat atattatact tttctcagga
      781 gaaagctaga tgtacagacg tttgagagat ttgagggaag atcacgattt gacccaaaaa
      841 caaatagcta aaatactttc gtttacaaac tcagcttatg ctaaaattga acggggtgag
      901 catacgttaa cagcagatgt attggtaact ctctcaaact tttacgatgt cagtacagac
      961 tacctattgg aattgacaga ttttcctgat aaaattcgct ttagaaaata atctcctcaa
     1021 tttcatagag tgtgaaaatg aggagatttt ttatttgttc tttgaaaatt aaatagtcca
     1081 aaatggtact ttcctcattt gtggagcaaa tttgaatggc tcgccatgat aagagcgatt
     1141 ttaaaaccat caataaaata gagcgatact ttatatgcca tgatacaaat gatatacaat
     1201 gatacttctg accgttcagg ctgccatcgt aaaagagcag caagtgaaat tcttatgatg
     1261 acttcatcag tcatgccacg ttgaatgtgt gagtttgtta gagaaacgca attaatcctc
     1321 aaaaggttcc ccgaaccttt tgagttccac agacgcatca cgtggagtgt gtaagcttgt
     1381 tgctaaaagc gtaaaaacct tggaacgaaa ggaataatag acttcctgcg aaacaaaaat
     1441 acaatacaat aaaactatgg atgatgaagc aagtaaacaa ttaagcgata gccgtttcaa
     1501 gagccttgta ggtgttcagc gcacgagatg ttagctgtgt taaaaacagc ttatcaacgc
     1561 aacgtaaacg cgcaaaaggt ggacgaaaaa ccaaattaag cctagacgat ctcctcatgg
     1621 taactattca atacatgcga gaatagcgca cttatgaaca aattgcggct gattttggca
     1681 ttcacgaaag caacataatt agtcggaggt caatggattg aagcaactct tattcaaaat
     1741 ggttttacga tttcaaattc tgccttaatt ctgtaaaaac agtaaaattc gaaggattgt
     1801 aaggtaagag tttttttctt tctgaaaaaa tggtataata gcaatcaaaa ctagaaaata
     1861 aaacggaatt tggaacagat ttgtctgtat cctagtagag tggtgatact atgaagatta
     1921 gtaagaggca cttattaaat tattccatct tgattcccta cttgctttta tctattttgg
     1981 gcttgattgt ggtctattcg accaccagtg ctattttaat tgaagaaggc aagagcgcct
     2041 tgcagttggt tcgaaaccaa ggaatctttt ggattgttag tttgatactg attgccttaa
     2101 tttataaatt gagactagat tttttgagaa atgagcgact aatcatttta gttatattaa
     2161 tagaaatgct tttattgttc ttggctcgtt ttattggtat ttcagtaaac ggggcatacg
     2221 gttggatttc ggttgcagga gtaactattc agccagctga gtacttaaaa atcattatta
     2281 tttggtattt agctcaccga ttctccaaac agcaagaaga aatagctact tatgattttc
     2341 aagttttgac tcaaaatcaa tggcttcccc gtgcttttaa tgattggcga ttcgttctcc
     2401 tagttctgat tggaagtttg ggaattttcc ctgatttagg aaatgcgact attttagtct
     2461 tggtttcctt gattatgtat acagttagtg gaatcgctta tcgctggttt tcaaccattc
     2521 tggcgctcgt atctgccgct tctgtctttg tcttgaccac tatcagccta atcggtgttg
     2581 agaccttttc aaaaattcca gtatttggct atgtagccaa acgctttagt gcctttttta
     2641 atccttttgc cgatcgtgct gatgcaggtc accagttagc taattcttat tttgccatgg
     2701 tcaatggtgg ttggtttggt ctaggtcttg gaaactcgat tgaaaaacga ggttatttgc
     2761 cagaagctca tacagacttt gtcttttcta tcgtgattga agaatttggc tttgttggtg
     2821 ccagtcttat tttagctctc ttgtttttca tgattttgcg gattatcttg gtcggtattc
     2881 gagcggagaa tcctttcaat gccatggttg cactcggtgt cggagggatg atgttggttc
     2941 aggtatttgt caatatcgga gggatttcgg gcttgattcc atctacagga gtaaccttcc
     3001 ccttcttatc ccagggtgga aatagtcttc tagtcttatc agtggcagta gcctttgtct
     3061 taaatattga tgccagtgaa aaacgcgcta agttgtaccg agaattggaa aatcaaccaa
     3121 tgaaccttct gttgaagtag gataaagaaa ggatagttta tgtctcttca aaaattagaa
     3181 aattctagta ataaaagtgt tgtgcaagaa gaagtcttga ttctaacaga attactggaa
     3241 gatattacta aaaatatgct tgccccagag acctttgaaa aaataataca gttgaaagaa
     3301 ttatcaacgc aggaagatta tcaaggtcta aaccgtctag tgactagctt atcaaatgat
     3361 gaaatggtct atatttcacg ctatttctct atcttgcctc ttttgattaa tatttcagag
     3421 gatgtggatt tagcttatga aatcaatcat caaaataata ttgatcagga ctatttaggt
     3481 aaattatcta caacgattaa attggtagca gaaaaggaaa atgccgttga gatcctagaa
     3541 cacttgaatg ttgttccggt tttgacagcc catccaacac aagtgcaacg caaaagtatg
     3601 ttggatttaa caaatcatat tcatagtctt ttgcgtaaat accgtgatgt taagttgggg
     3661 ttgatcaata aagataaatg gtacaatgat ttgcgtcgtt acatcgaaat tatcatgcag
     3721 acagacatga ttcgtgagaa aaaattaaaa gtgactaacg aaatcacgaa tgctatggaa
     3781 tattataaca gctccttttt gaaagctgta cctcatttga cgacggagta taagcgctta
     3841 gcgcaagcgc atggtctgaa tttaaaacag gctaaaccaa tcaccatggg tatgtggata
     3901 ggtggtgacc gtgatggaaa tccatttgtt acagcaaaga ccttgaagca gtctgcactc
     3961 actcagtgtg aagtcatcat gaactactat gataaaaaga tttaccaact ttatcgtgaa
     4021 ttttctcttt caactagcat tgtcaacgtc agcaaacaag tcagagaaat ggctcgtcaa
     4081 tccaaggata actcgattta ccgcgaaaaa gagctttacc gtcgtgcctt gtttgatatt
     4141 caatcaaaaa ttcaggcaac taaaacctat ctgattgagg atgaagaagt tgggactcgt
     4201 tatgaaaccg ccaatgattt ctacaaggat ttgattgcca ttcgagattc tctactagaa
     4261 aataagggcg agtccttgat ttcaggtgat tttgtggaat tattgcaggc agtagagata
     4321 tttggttttt acttagcatc aattgatatg cgacaagact ctagcgtcta tgaagcctgt
     4381 gtggcagaac tcttgaaatc agcaggaatt cattctcgtt atagcgagtt gagcgaagaa
     4441 gaaaagtgtg accttctctt gaaagaatta gaagaagatc cccgaattct ttctgcgact
     4501 cacgcagaaa aatcagaatt attagcaaaa gaactagcta tttttaagac ggctcgtgtt
     4561 ttgaaagata agttgggaga tgatgtcatc cgtcagacca tcatttcaca tgcaaccagc
     4621 ctttctgata tgctagaatt agctattctg ttaaaagaag taggactggt ggatacggaa
     4681 agggcgcgtg ttcagattgt tccccttttt gaaacaattg aagacttgga tcattcagag
     4741 gaaacaatga gaaaatatct ttctcttagc cttgccaaaa aatggattga ctcacgaaat
     4801 aactaccaag aaatcatgct tggctactct gacagtaata aagatggcgg ttacttgtca
     4861 tcatgttgga ccctctacaa ggctcaacaa caattgactg ctattggaga tgaatttggc
     4921 gttaaggtta ccttcttcca tggtcgtggt ggtactgtcg gtcgtggtgg tgggccaacc
     4981 tatgaagcca ttacatctca accgctcaag tctatcaagg atcgtatccg cttgacggag
     5041 cagggtgaag taattgggaa taaatacggt aacaaagacg ccgcttacta taaccttgaa
     5101 atgctagtat cggcagctat taaccgtatg attactcaga agaagagcga taccaatacc
     5161 ccaaatcgtt atgaagccat tatggatcaa gtagtggacc gtagttacga tatctaccgt
     5221 gatttggtct ttggtaatga gcatttctat gattatttct tcgagtcaag tccaatcaag
     5281 gctatttcaa gttttaatat tggttctcgt ccagccgctc gtaagactat tactgaaatc
     5341 ggtggattgc gtgctatccc ttgggtattc tcatggtcac agagtcgtgt tatgttccct
     5401 ggatggtacg gggttggttc aagcttcaag gaatttatca ataaaaatcc agagaatatt
     5461 gctatcttac gagatatgta ccaaaattgg cctttcttcc aatcgcttct ttcaaatgtt
     5521 gatatggttt tgtcaaaatc aaatatgaat attgcttttg aatatgctaa actttgtgaa
     5581 gacgagcaag ttaaggccat ctatgagact attttaaatg aatggcaagt tactaagaac
     5641 gttatcttgg ctattgaagg acatgacgaa ctcttagctg acaatccata tctaaaagct
     5701 agtctggatt accgtatgcc ttactttaat attctcaact atattcagtt agagttgatt
     5761 aaacgccaac gtcgtggaga attgtccagt gatcaagaac gattgattca tatcaccatc
     5821 aacggaattg cgacaggatt gcgtaattca ggttgataat tttcaagagt gaatgctaaa
     5881 agtgaatatc aaaaaaattc taatagacta ttgacaagta gtttaaaaat gatataattt
     5941 aaccattcag aaaagtaatc atacaaactt tttagagagt ctgtggtagc tgaaaacaga
     6001 taagtggcaa tgatgaaaat tgggctgaat gctatttaga atttgaaatt ataaaaattc
     6061 ggtaagcaca ccttacagtg catctcgtta ttgcgagact gagcgatagg gaaattccct
     6121 ataattgagg tggtaccgcg catcgacgtc ctcacacaag ttttttgtgt gaggattttt
     6181 ttgatggagg ttagtatgga aagaaaacga tggcgtcgct tgtttagata agtgaaatat
     6241 gttaaaggaa ataaaaagga gaaacagaat gaaaaataaa cgtttaattg gaattattgc
     6301 tgcattagca gtcttagtag caggaagctt gatttattct tcaatgaata aatcagaagc
     6361 tcagaataat aaggatgaga agaaaataac caagattggt gtgcttcaat ttgtgagcca
     6421 tccatccctt gatttgattt ataaagggat ccaagatgga cttgcagaag aaggatataa
     6481 agatgatcaa gttaaaattg attttatgaa ctcagaaggt gaccaaagta aggttgcgac
     6541 aatgagtaaa caattggttg caaatggaaa tgaccttgtg gttggtatcg caacaccagc
     6601 agcccaaggg ttggctagtg caacaaaaga cctaccggtt atcatggccg ctattacaga
     6661 cccaattggt gctaacttgg ttaaagattt gaaaaaacca ggtggcaaca ttacaggggt
     6721 atctgaccac aatccagctc aacaacaagt tgaactcatc aaggctctga caccgaatgt
     6781 gaaaacaatc ggagctcttt actcaagtag cgaagacaat tcaaaaacac aggtcgaaga
     6841 atttaaggct tatgctgaaa aagcaggtct gacagtggaa acatttgcag ttccttcaac
     6901 aaatgaaatt gcctcaactg tcactgttat gactagcaag gtagatgcta tttgggttcc
     6961 aattgataac accattgcat caggatttcc aacggttgtc tctagcaatc aaagttctaa
     7021 gaaaccaatt tatcccagtg cgacagctat ggtagaagta ggtggtttgg catcagttgt
     7081 aattgaccaa catgaccttg gtgtggcaac aggtaaaatg attgtgcaag tcttgaaagg
     7141 tgcaaaacca gccgataccc cagtcaatgt cttttcaact ggtaagtcag tcatcaataa
     7201 aaaaatagca caagaactag gtattactat tcctgagtct gttctcaaag aagcaggaca
     7261 agtcatcgaa taaataaggt agggaggagg agactcttcc cattttttaa aaagatgaat
     7321 atagaaagga ttaaataaaa tatgattgtt tccattattt ctcaaggatt tgtctgggct
     7381 attctaggtc tgggaatctt tatgacattt aggattttaa actttccaga tatgacgaca
     7441 gaaggttcct tccctcttgg gggagctgtt gctgtcactt tgataaccaa aggcgtgaac
     7501 ccatttttag cgacacttgt tgctgtagga gcaggttgtt tggctggaat ggcagcaggc
     7561 cttctttata caaaagggaa gatcccaacc ttgctctcag ggattttggt gatgacttct
     7621 tgtcactcaa tcatgctctt gattatggga cgtgcgaatt taggcctgct tggaaccaag
     7681 caaattcagg atgttttgcc ttttgattcg gatttgaatc aactcttgac aggtctcatc
     7741 tttgtgagta ttgttattgc tctcatgctc tttttcttgg acactaaact cggacaagcc
     7801 tatattgcta caggggataa tcctgatatg gctagaagtt tcgggattca tactggacgc
     7861 atggagctca tgggcttggt cttatcaaat ggtgtgattg cccttgcagg tgccctcatt
     7921 gctcagcaag aaggctatgc cgatgtgtct cgagggatcg gggttatcgt tgtggggctt
     7981 gcaagtttga ttattggaga agttattttc aagagtttga gcttggcaga gcgtttggtt
     8041 actatcgttg taggttctat cgcttatcaa tttttagtgt gggcagttat cgcacttggc
     8101 tttaatacaa gttaccttcg tttatacagt gccttgattt tagcagtctg cctcatgatt
     8161 ccaacattta agcaaacaat cttgaaagga gccaagttaa gcaaatgaca gcaattgtag
     8221 aattaaaaaa tgcaaccaaa atcgttaaaa atggctttga tgaagaaaag attattttaa
     8281 atgatgtttc cttagaaatt tttgaacggg actttatcac gattttgggc ggaaatggtg
     8341 ctggaaaatc aaccctcttt aacactatag cagggacctt atcactaact agtggaacta
     8401 tccgtatttt aggtgaagaa ctcactaagt tttcacccga gaagcgtgcc aagtacctgt
     8461 ctcgtgtctt ccaagatcca aagatgggga cagctccccg tatgacggtc gctgaaaatc
     8521 ttttaatcgc caagtttcgt ggtgaaaagc gtggattgtt accacgacgc ttgactagct
     8581 ataaggatga atttcaggca accattgaaa aagtaggaaa tggtcttgag aaacacttga
     8641 atacaccgat tgagttctta tcaggtggac aaagacaggc tttgagtctc ttgatggcaa
     8701 ccttgaagcg acctgaatta ctcctgttag atgagcatac tgctgccctg gatccaaaga
     8761 ctagtgttgc tttgatggaa ttgacagatg aatttgttaa gaaagatcag ctaacagccc
     8821 ttatgattac tcatcatatg gaagatgctc tcaaatacgg caatcgcttg attgtcatga
     8881 aagaaggacg gattatccaa gatttaaacc aagaagaaaa agcaaaaatg aaaatctctg
     8941 attattatca actctttgaa taaagtaggg aaaatgagtg aaatggttta ctttttttct
     9001 gaaataaagt atactatata aagtaaacta tgataacatg gaggtattgt gtatggttga
     9061 caaacaagtc attgaagaaa tcaaaaacaa tgccaacatt gtggaagtca taggagatgt
     9121 gatttcttta caaaaggcag gacggaacta tctagggctc tgtccttttc atggtgaaaa
     9181 aacaccttct ttcaacgttg tagaggacaa gcagttttac cactgttttg gttgtggtcg
     9241 ctcaggtgat gtctttaaat tcatcgagga gtaccaaggg gttaccttta tggaggctgt
     9301 ccaaatctta ggtcagcgtg tcgggattga ggttgaaaaa ccgctttata gtgaacagaa
     9361 gccagcctcg cctcaccaag ctctttatga tatgcacgaa gatgcggcta aattttacca
     9421 tgctattctc atgacaacga ctatgggcga agaggccaga aattaccttt atcagcgggg
     9481 tttgacagat gaagtgctta aacatttttg gattggttta gcacctccag aacgaaacta
     9541 tctctatcaa cgtttgtctg atcagtatcg tgaagaagat ttactggatt caggcctgtt
     9601 ttatctctcg gatgccaatc aatttgtaga cacctttcac aatcgcatta tgtttcccct
     9661 gacaaatgac caaggaaagg tcattgcctt ctcaggtcgt atctggcaaa aaacagattc
     9721 acaaacttct aagtataaaa acagccgatc gactgcaatt tttaacaaaa gttacgaatt
     9781 atatcatatg gatagggcaa aaaaatcttc tggaaaaact agtgagattt acctgatgga
     9841 aggattcatg gatgttattg cagcctatcg ggctggaatc gaaaatgctg tggcgtcgat
     9901 gggaacggcc ttgagtcgag agcatgttga gcatctgaaa aggttaacca agaaattggt
     9961 tcttgtttac gatggagata aggctgggca agccgcgata ttgaaagcat tggatgaaat
    10021 tggtgatatg cctgtgcaaa tcgttagcat gcctgataac ttggatcctg atgagtatct
    10081 acaaaaaaat ggtccagaag acttggccta tctattaacg aaaactcgta ttagtccgat
    10141 tgagttctac attcatcagt acaaacctga aaacagtgaa aatctgcagg ctcagattga
    10201 gtttattgaa aaaatagctc ccttgattgt tcaagaaaag tccatcgctg ctcaaaacag
    10261 ctatattcat attttagctg acagtctggc gtcctttgat tatgcccaga ttgagcagat
    10321 tgttaatgag agtcgtcagg tgcaaaggca gaatcgcatg gaaggaattt ccagaccgac
    10381 gccaatcacc atgcctatca ccaagcagtt atcggctatt atgagggcag aagcccatct
    10441 actctatcgg atgatggaat cccctcttgt tttgaacgat taccgtttgc gagaagactt
    10501 tgcatttgct acacctgaat ttcaggtctt atatgacttg cttggccagt atggaaatct
    10561 tcctccagaa gttttagcag agcagacaga ggaagttgaa agagcttggt accaagtttt
    10621 agctcaggat ttgcctgctg agatatcgcc gcaggaactt agtgaagtag agatgactcg
    10681 aaacaaggct ctcttgaatc aggacaatat gagaatcaaa aagaaggtgc aggaagctag
    10741 ccatgtagga gatacagata cagccctaga agaattggaa cgtttaattt cccaaaagag
    10801 aagaatggag taataatggc aacaaaacaa aaagaagtaa caacatttga cgtacaggta
    10861 gcagaattta tccgtaatca taagcaaaaa gggacagcaa cagatgatga aatcaatgca
    10921 agtctggtta ttccttttac cttggacgct gatgggattg aagatctctt gcaacggatt
    10981 caggatgcag gcatttctat cacagataac gaaggaaatc caagtgcgcg tattcttagc
    11041 aatgaagaag aaccagaact cagcgatgag gacttgattg ggtcaacttc tgctaaggtc
    11101 aatgaccctg tccgtatgta cttgaaagaa ataggggtcg ttcctctctt gaccaatgaa
    11161 gaggagaaag agttggcact ggctgttgaa gctggcgata tcgaagccaa acaacgtctt
    11221 gcggaagcca atcttcgttt ggttgtttct attgccaaac gctatgtcgg tcgtggcatg
    11281 cagttccttg acttgattca agaaggaaat atgggcttga tgaaggcggt tgacaagttt
    11341 gactattcta aagggttcaa gttttcaact tatgcaactt ggtggattcg tcaggctatc
    11401 actcgtgcta ttgcggatca agctcgtacc atccgtatcc cagttcacat ggttgaaact
    11461 atcaataaat tggttcgtga acagcggaat ctccttcaag aattggggca agatccgaca
    11521 ccagaacaga ttgctgaacg aatggatatg acacctgata aggttcgtga aatcttgaag
    11581 attgcccaag aaccagtatc tcttgaaact cctatcggtg aagaggacga tagccacctt
    11641 ggagacttta tcgaagatga agtgattgaa aatccagtgg attatacgac tcgtatcgtc
    11701 ttgcgtgagc aattggatga aatcttagat actcttacag accgtgaaga aaatgttctg
    11761 cgtctacgtt ttggactaga tgatggaaaa atgcgcacac ttgaagatgt ggggaaagtc
    11821 tttaacgtaa ctcgtgagcg tatccgtcag attgaagcaa aggctttgag aaaactacgc
    11881 caaccaagcc gcagcaaacc gcttcgtgat tttattgaag actaagagtg aggaaaatat
    11941 ggcttataca gaagagcaaa ttgaaaacat caaaacacgg attttaacag ccttggaaga
    12001 agtcatcgac cctgagttgg gaatcgatat tgtcaatctt ggtttgatct atgagattcg
    12061 ttttgacggt gacacagggc aaacagagat tgacatgact ttgacaacta tgggttgtcc
    12121 cttagcagac cttttgacag accagattta tgatgctatg atagaggttc cagaagtaac
    12181 ggatactgag gttaaattag tctggtatcc agcctggaca gttgaaaaaa tgagtcgcta
    12241 tgcccgcatt gccctaggca ttaagtaaac atataaaagc atgtgagaga ctgttggaaa
    12301 agcgaggaaa tttcccctct tttcctctag tctctccttt cttttgctga ttttattcaa
    12361 agaaaatgat ataatagtag ttatggagaa aaagaaatta cgcatcaata tgttgagttc
    12421 aagtgagaaa gtagcaggac agggagtttc aggtgcttac cgtgaattag ttcgtcttct
    12481 tcaccgtgct gccaaggacc aattgattgt tacagaaaat cttccaatcg aggcagatgt
    12541 gactcacttt catacgattg attttcccta ttatttatca accttccaaa agaaacgctc
    12601 agggagaaag attggctatg tgcatttctt gccagctaca cttgaaggaa gtttgaaaat
    12661 tccatttttc ttaaagggaa ttgtgaaacg ctatgtattt tctttttaca accggatgga
    12721 gcacttggtt gtggtcaatc ctatgtttat tgaggatttg gtagcagctg gtattccacg
    12781 tgaaaaagtg acctatattc ctaactttgt caacaaggaa aaatggcatc ctctaccaca
    12841 agaagaggta gtcagactgc gcacagatct tggtcttagt gacaatcagt ttatcgtagt
    12901 aggtgctggg caagttcaga aacgtaaagg gattgatgac tttatccgtc tggctgagga
    12961 attgcctcag attaccttta tctgggctgg tggcttctct tttggtggta tgacagatgg
    13021 ttatgaacac tataagaaaa ttatggaaaa tccccctaaa aatttgattt ttccaggcat
    13081 tgtatcgcca gagcggatgc gcgaattgta tgctctagcg gatcttttct tgttgcctag
    13141 ttacaatgag ctctttccta tgactatttt agaagctgcg agttgtgagg ctcctattat
    13201 gttgcgtgat ttagatctct ataaggtgat tttggaggga aattatcggg cgacagcggg
    13261 tagagaagag atgaaagagg ctattttgga atatcaagca aatcctgctg tcttaaaaga
    13321 tctcaaagaa aaggctaaga atatttccag agagtattct gaagagcatc tgttacaaat
    13381 ctggttggac ttttatgaga aacaagccgc tttagggaga aagtaaaaag tgaggtaatc
    13441 tatgcgaatt ggtttattta cagataccta ttttcctcag gtttctggtg ttgcgaccag
    13501 tattcgaacc ttgaaaacag aacttgaaaa gcagggacat gctgttttta tctttacgac
    13561 gacagataag gatgtcaatc gctacgaaga ttggcaaatt atccgcattc caagtgttcc
    13621 tttctttgct tttaaggatc gtcgctttgc ctaccgaggt tttagcaagg cacttgaaat
    13681 tgctaaacag tatcagctag atattatcca tactcagaca gaattttctc ttggcctgtt
    13741 ggggatttgg attgcgcgtg aattgaaaat tccagtcatc catacctatc acacccagta
    13801 tgaagactat gtccattata ttgctaaggg gatattgatc cggcctagta tggtcaagta
    13861 tctggttaga ggtttcctgc atgatgtgga tggggttatt tgccctagtg agattgtccg
    13921 tgacttgcta tctgattata aggtcaaggt tgaaaaacgg gtcattccta ctgggattga
    13981 attagccaag tttgagcgtc cggaaatcaa gcaggaaaat ttgaaagaac tgcgtagtaa
    14041 actagggatt caagatggtg aaaagacgtt gcttagtctt tcgagaatct cctatgaaaa
    14101 aaatattcaa gcagttttag cagcctttgc tgatgttctg aaagaggaag acaaggttaa
    14161 actggtagta gctggggatg gcccttatct gaatgacctc aaagagcaag cccagaacct
    14221 agagattcaa gactcagtca tctttacagg gatgattgct cctagtgaga cggctcttta
    14281 ctataaagcg gcggatttct tcatttcggc atcgacaagc gaaacgcaag gtttgaccta
    14341 cttggaaagc ttagccagtg gaacacctgt cattgctcac ggaaatcctt atttgaacaa
    14401 cctcatcagt gataaaatgt ttggagcctt gtactatgga gaacatgatt tggctggtgc
    14461 tattttggaa gccctgattg caacaccaga catgaacgag cataccttat cagagaaatt
    14521 gtatgagatt tcagctgaga actttgggaa acgagtgcat gagttttatc tggatgccat
    14581 tatttcaaat aacttccaga aagatttggc taaagatgat acggtcagtc agcgtatctt
    14641 taggacagtt ttgtatcttc cgcaacaggt ggttgctgta cctgtaaaag gatctagacg
    14701 catgttgaag gcttcaaaaa cacagttgat cagtatgaga gactattgga aagaccatga
    14761 agaatagaaa gaggaacagc tatgaaaaaa acaattaatg agaagcggtc gtgataaaaa
    14821 gattgcgggt gtttgtgctg gggtggccca ttatctggat atggatccga ctatcgttca
    14881 agtcatttgg ggtgttctta cttgctgtta cggagctgga attgtagctt acattatttt
    14941 atggattatc gcaccagtag caactgacta ttgaaaacta gctcaattca tgctaagata
    15001 atagaaaata gaatgtaacg aaggagagaa aaatggcatt tggagataat ggaaatcgta
    15061 aaaaaactat gtttgagaaa ataaccttgt ttatcgtgat tatcatgcta gtagcaagtt
    15121 tattgggaat ttttgcaact gcaattggtg ccctcagtaa tctataaaat agattcaaga
    15181 aaatttagtg actgggattt cccagccctt ttttaaagtg agaagaaata atgagtatgt
    15241 ttttagatac agctaagatt aaggtcaagg ctggtaatgg tggcgatggt atggttgcct
    15301 ttcgtcgtga aaaatatgtc cctaatggag gcccttgggg tggtgatggt ggtcgtggag
    15361 gcaatgtggt cttcgttgta gacgaaggac tacgtacctt gatggatttc cgctacaatc
    15421 gtcatttcaa ggctgattct ggtgaaaaag ggatgaccaa agggatgcat ggtcgtggtg
    15481 ctgaggacct tagagttcga gtaccacaag gtacgactgt tcgtgatgcg gagactggca
    15541 aggttttaac agatttgatt gaacatgggc aagaatttat cgttgcccac ggtggtcgtg
    15601 gtggacgtgg aaatattcgt ttcgcgacac caaaaaatcc tgcaccggaa atctctgaaa
    15661 atggagaacc aggtcaggaa cgtgagttac aattggaact aaaaatcttg gcagatgtcg
    15721 gtttagtagg attcccatct gtagggaagt caacactttt aagtgttatt acctcagcta
    15781 agcctaaaat tggtgcctac cactttacca ctattgtacc aaatttaggt atggttcgca
    15841 cccaatcagg tgaatccttt gcagtagccg acttgccagg tttgattgaa ggggctagtc
    15901 aaggtgttgg tttgggaact cagttcctcc gtcacatcga gcgtacacgt gttatccttc
    15961 acatcattga tatgtcagct agcgaaggcc gtgatccata tgaggattac ctagctatca
    16021 ataaagagct ggagtcttac aatcttcgcc tcatggagcg tccacagatt attgtagcta
    16081 ataagatgga catgcctgag agtcaggaaa atcttgaaga atttaagaaa aaattggctg
    16141 aaaattatga tgaatttgaa gagttaccag ctatcttccc aatttctgga ttgaccaagc
    16201 aaggtctggc aacactttta gatgctacag ctgaattgtt agacaagaca ccagaatttt
    16261 tgctctacga cgagtccgat atggaagaag aagcttacta tggatttgac gaagaagaaa
    16321 aagcctttga aattagtcgt gatgacgatg cgacatgggt actttctggt gaaaaactca
    16381 tgaaactctt taatatgacc aactttgatc gtgatgaatc tgtcatgaaa tttgcccgtc
    16441 agcttcgtgg tatgggggtt gatgaagccc ttcgtgcgcg tggagctaaa gatggggatt
    16501 tggtccgcat tggtaaattt gagtttgaat ttgtagacta ggagactggt atgggagata
    16561 aaccgatatc tttccgagat gcggatggta attttgtttc cgccgcagac gtttggaatg
    16621 aaaagaaatt ggaagaacta tttaatcggc tcaatccaaa tcgtgccttg agattggcac
    16681 gaactaaaaa ggaaaatcca tctcagtaaa gaagctaaaa aatccggctc tttgtcaact
    16741 gtagtaggtt gaagaa
//
LOCUS       DAVKWZ010000042        16741 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000042 DAVKWZ010000000
VERSION     DAVKWZ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16741
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.042"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(<1..512)
                     /locus_tag="VW854_001484"
     CDS             complement(<1..512)
                     /locus_tag="VW854_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0262702.1"
                     /translation="MEQLHFITKLLDIKDPNIKILDIINMDTHKEIIAKLDYDAPSCP
                     ECGNQLKKYDFQKPSKIPYLETTGMPTRILLRKRRFKCYHCSRMMVAETSIVKKNHQI
                     PRIINQKIAQKLIEKISMTDIAHQLAISTSTVIRKLNDFHFKHDFSCLPEIMSWDVET
                     VRGVTVSIGR"
     gene            complement(667..1377)
                     /gene="treR"
                     /locus_tag="VW854_001485"
     CDS             complement(667..1377)
                     /gene="treR"
                     /locus_tag="VW854_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEV0262703.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            1562..3529
                     /gene="treP"
                     /locus_tag="VW854_001486"
     CDS             1562..3529
                     /gene="treP"
                     /locus_tag="VW854_001486"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEV0262704.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKIEGDTNLQAEFVAQEEAEFVSYEPVELTSVEII
                     SPLTGQVKELSQATDPVFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVIVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATITGTYPTTIQAGASLMVATRI"
     gene            3707..5332
                     /gene="treC"
                     /locus_tag="VW854_001487"
     CDS             3707..5332
                     /gene="treC"
                     /locus_tag="VW854_001487"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEV0262705.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNPNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKDINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            5474..5722
                     /locus_tag="VW854_001488"
     CDS             5474..5722
                     /locus_tag="VW854_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEV0262706.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            5929..6723
                     /gene="racE"
                     /locus_tag="VW854_001489"
     CDS             5929..6723
                     /gene="racE"
                     /locus_tag="VW854_001489"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEV0262707.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            6720..7691
                     /locus_tag="VW854_001490"
     CDS             6720..7691
                     /locus_tag="VW854_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEV0262708.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            7667..8188
                     /locus_tag="VW854_001491"
     CDS             7667..8188
                     /locus_tag="VW854_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV0262709.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            8185..8646
                     /locus_tag="VW854_001492"
     CDS             8185..8646
                     /locus_tag="VW854_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV0262710.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            8637..9377
                     /gene="xerD"
                     /locus_tag="VW854_001493"
     CDS             8637..9377
                     /gene="xerD"
                     /locus_tag="VW854_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEV0262711.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISVQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            9377..10105
                     /locus_tag="VW854_001494"
     CDS             9377..10105
                     /locus_tag="VW854_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEV0262712.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            10124..10693
                     /locus_tag="VW854_001495"
     CDS             10124..10693
                     /locus_tag="VW854_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEV0262713.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            10680..11402
                     /locus_tag="VW854_001496"
     CDS             10680..11402
                     /locus_tag="VW854_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV0262714.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            11402..11644
                     /gene="yidD"
                     /locus_tag="VW854_001497"
     CDS             11402..11644
                     /gene="yidD"
                     /locus_tag="VW854_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEV0262715.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFDLTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(11869..12834)
                     /locus_tag="VW854_001498"
     CDS             complement(11869..12834)
                     /locus_tag="VW854_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV0262716.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEITI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETSTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(12930..13682)
                     /locus_tag="VW854_001499"
     CDS             complement(12930..13682)
                     /locus_tag="VW854_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV0262717.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINNKKICIYS"
     gene            complement(13679..14635)
                     /locus_tag="VW854_001500"
     CDS             complement(13679..14635)
                     /locus_tag="VW854_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEV0262718.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRSRQ"
     gene            complement(14625..15584)
                     /locus_tag="VW854_001501"
     CDS             complement(14625..15584)
                     /locus_tag="VW854_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0262719.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFRGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            complement(16007..16708)
                     /gene="galE"
                     /locus_tag="VW854_001502"
     CDS             complement(16007..16708)
                     /gene="galE"
                     /locus_tag="VW854_001502"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV0262720.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYKVHVLDNLSTGNRAAVG
                     SRASFKELDVYDASALKAYLEENQIDAVLHYAGEIVVSESIENPSKYFTANVAGMDQV
                     LKVLSEVGIQKIMFSSTVSLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
BASE COUNT         4703 a         3320 c         3866 g         4852 t
ORIGIN      
        1 catctcccga ttgaaacagt cactcctcgg actgtttcaa cgtcccagga cataatctca
       61 ggaagacaag aaaaatcatg tttaaagtga aaatcattga gctttcgaat gacagttgaa
      121 gttgaaatgg ccagctgatg agcaatatcg gtcatagaaa tcttttcaat taacttttgc
      181 gcaatttttt gattgataat acgagggatt tggtgattct tcttgacgat agaagtttca
      241 gcgaccatca ttcttgaaca gtgatagcac ttgaatcgac gctttctaag gagaattcta
      301 gtaggcatac cagttgtctc aaggtaagga atcttagacg gtttttgaaa gtcatatttc
      361 ttcaattggt ttccgcactc agggcaagat ggggcgtcgt agtccagttt ggcgatgatt
      421 tctttgtggg tatccatatt gatgatatct agaatcttga tgtttgggtc tttaatatcg
      481 agcagttttg tgataaaatg taattgttcc atatgaatct ttctaatgag ttgttttgtc
      541 gcttttcatt ataggtcata tgggactttt tttctacaca aaataggctc cataatatcc
      601 ataggggggt tacccactac aaatattata gagccgaaaa aggctgagat ctggtgtctc
      661 agtcttttat ttcttgcgtt ttgcaaaatc tacaaatcta aatttctcta acttatggcg
      721 actttcggta aactgaaatt ggcgtccgtc ttggagatag acttttgatt taatcgaaac
      781 gacataaggg tctttgccaa tgtccatgag aatcttgtct cggtcgcttg agtggtcaat
      841 ggtgatttcc ttctgagcat aatcaataag gagtttgagg ccattttcta tataagaata
      901 gatagactgc tcagcaattt ggcgagtgag atttggaatt agttccatat ccagatagtc
      961 cgtatccaga acggatacca gatcatccac cacacgctgg cggaccacct tccaaaccat
     1021 ccgaaactct gggaaaccgg ttatcagtga ggatttttta tcaataataa tcttgtccag
     1081 actgaccacg ttggttttag agcgcagtcc aagttcttta actagttctt ggtagctggt
     1141 taggttggat acagggaaat tgacggtttc ttctttgacg acttgagaac cttgccctct
     1201 tatctttttg atcaatcctt cctcttggag gagagacagg gcctttcgga cggtatcacg
     1261 actcacttga tattgctcca taaggtcgtg ctcgctagga aggaaatctc cgacagcgta
     1321 agtctcgttt tgaatggttt cttggatttg cttaaataat tgttggtatt tcttcatttt
     1381 gcctgcttaa ataggttttt agtctagttt aatgaacacc tgtatctatt ttaccataca
     1441 agtgctagaa aatttacaaa aaaacaccct ttttatacca aacaagttgc agacaagttt
     1501 ggtatcgttt acaatatact tatcaaatca aacttgcttt gacaagtata aggagaatca
     1561 aatgggaaaa tttgaacaag aagccaaaga tctgcttcag gcaattggag gcaaagaaaa
     1621 tgtgactgcc gtaactcact gtgcgacacg gatgcgcttt gttttaggag atgataagaa
     1681 ggctaatgtt aaagctatcg agtcaattcc agctgttaaa ggaactttta caaatgcagg
     1741 tcaatttcag gtaatcattg gaaatgacgt gcccatcttt tataatgatt ttacagccgt
     1801 ttcaggtatt gagggtgttt ccaaagaagc agccaagtct gcagctaaga gtaatcaaaa
     1861 cgtggtccaa cgtgttatga ccactctggc ggagattttt actccgatta ttccagcctt
     1921 gatagtcgga ggattgatcc tcggtttccg taatgtcttg gaaggtgtcc attggtcgat
     1981 gttggatggc aagaccatca cagaatcctc tcagttttgg gcaggtgtca atcacttcct
     2041 ctggttgcct ggtgaagcta tcttccagtt cttaccagta gggattactt ggtctgtttc
     2101 tcgtaagatg ggaaccagcc aaattttggg aattgttctc ggaatctgtt tggtatcgcc
     2161 tcagttgctc aatgcctatg cggttgcttc aacgccagca gctgatatcg cagcaaactg
     2221 ggtttggaat tttggttatt ttactgttaa tcgtattggt taccaagccc aagttatccc
     2281 agccttgctt gcaggtttga gtctgtctta tcttgaaatt ttttggcgca aacatatccc
     2341 agaagtcatt tccatgattt ttgtaccttt tttgtcattg attccagcct tgattttggc
     2401 tcatactgtc ttggggccaa tcggttggac aattggacaa ggactttcat cagttgtatt
     2461 ggcaggttta acgggtcctg taaaatggct cttcggtgca atttttggtg ccctctacgc
     2521 tccatttgtc atcacaggtc tgcaccacat gaccaatgcc attgatacac aattgattgc
     2581 ggatgctggt ggcactgccc tctggcctat gattgctctt tctaatattg ctcaaggctc
     2641 agccgtgttt gcctattatt tcatgcatcg ccatgatgag cgtgaggctc aggtttcact
     2701 tcctgcaacc atttcagcct atctcggtgt tacagaacca gctctttttg gggttaacgt
     2761 aaaatatatt tatccatttg ttgctgggat gactggttca gcccttgcag gcatgttatc
     2821 cgttactttt aatgtaactg cggcttctat tggtatcggt ggtttgccag gtattctctc
     2881 tattcaacct caatacatgc tgccatttgc aggaactatg ctagttgcga ttgttgttcc
     2941 aatgctcttg actttcttct ttcgcaaggc tggtctcttc acaaaaatag agggcgatac
     3001 gaacttgcag gcagaattcg ttgctcaaga agaagcagaa tttgtgagct atgaaccagt
     3061 agaacttact tcggtagaaa ttatcagccc actaactggc caagtgaaag aattgagtca
     3121 agcgacggat cctgtttttg catcaggtgt catggggcaa ggtctggtca ttgaaccaag
     3181 ccaaggtgag ttgacctctc cagttaatgg aacagtgacg gttcttttcc ctaccaagca
     3241 tgccatcggc attgtctctg acgagggagt tgaattgctc atccacatcg gtatggatac
     3301 agtaggtctt gatggcaaag gttttgaaag tcttgtagtc caaggagatc acgttatagt
     3361 tggccagcaa ctgattcgtt ttgatatgga tgtcattaag gctgcaggtc tggtgacaga
     3421 aactcctgtt atcatcacca accaagatgc ttatacagcg actattactg gaacttatcc
     3481 gacaacgatc caagctggag catctctcat ggtcgctaca cgaatctaat actcttcgaa
     3541 aatctcttca aaccacgtca gctctatctg caacctcaaa atagtgtttt gagcaacctg
     3601 tggctagctt cctagtttgt tctttgattt tcattgagta taattgaaat agcacaaata
     3661 gggacggaag actgcttcct cccttttatc agaaaaggag aaacagatga cacttgataa
     3721 aggaaaagta gtctatcaaa tctatccaaa atcttacaaa gacaccactg aaaacggtat
     3781 tggggatttc cgtgggatta tcgaaaaaat tccctattta gccaaactag gtgtggatat
     3841 ggtctggctc aatccattct atccaagccc tcaacgggat aatggttacg atatttcaga
     3901 ttatatggca gtggatcctc tttttggtga tatggctgat tttgaggaaa tggtgtgtgt
     3961 cggtaaagag cacaagattg actttatgct ggacatggta cttaatcatt gttcgacaga
     4021 gcatgaatgg tttcagaaag ccctagctgg tgataagtat tatcaagact ttttcttcat
     4081 ccaagaccaa ccaacagatt ggcagtctaa gtttggtggt tctgcatggg cgcctttcgg
     4141 ggatactggg aaatactatc ttcacctatt tgatgagacc caggctgacc ttaactggcg
     4201 caatcccaat gtccgtaagg agcttttcaa ggttgttaat ttctggcgcg acaagggtgt
     4261 caaaggtttt cgatttgatg tgatcaattt gatcggtaag gacgaggttt cagtggactg
     4321 tcctgaaaat gaagggaagc cagcttatac agacaaaccc atcgttcata actatttgcg
     4381 tatgatgaac caagccactt ttggatctga cgatagcttt atgacagttg gggaaatgtc
     4441 ttctaccacc atggaaaact gtgtcctcta ttcgtcgcct gatcgtcagg aattatccat
     4501 gacctttaat tttcatcacc tcaaggtgga ctataaggat ggacaaaagt ggaccttggc
     4561 tccctttgat tttgaagagt tgaagagtct ttaccatagt tggggtaagg aaatgagtga
     4621 taaagacggt tggagtgccc tcttttggaa caatcacgat caaccacgtg ccttgaaccg
     4681 ttttgtcgat attcaaaact tccgcaagga aggagccact atgctagcag ccagcattca
     4741 cctgtcacgt gggacacctt atatctacat gggtgaggaa atcgggatga ttgacccaga
     4801 ctatgattct atggctgatt acgtggatgt cgaatcgctt aatgcctatc agatgctcct
     4861 agaagaagga aagagtcagc aagaagcctt ccagattatt caagctaagt cgcgtgataa
     4921 ttcacgcatt cccatgcagt gggatgcttc ggaaaatgca ggattttcaa caggcactcc
     4981 ttggttgaaa gctggtaaat cctataaaga catcaatgta gaaaatgaaa tccagggtcc
     5041 gatttttacc ttctatcaag acttgattcg actgcgtaag gaaatgccaa tcatttcaga
     5101 aggaagttac aaaccagcct ttgaagatag caaacaagtc tacgcttttg aacgccagtt
     5161 tgaggatcaa aagttactag tgctcaataa cttttatgct aaagaagtgg aaatcgactt
     5221 gccagcagtc taccaaaatg gacagatttt gatttcaaac tatgaagatg cagaagtgtc
     5281 tgagaaaatt ctactaaaac cctatcaaac actggctatt tatgtaaatt aaacttagca
     5341 aggagacctc tttttcagaa aagcagggtt ctttttgctg tctattccat ccttgaaagt
     5401 gtagaacaaa ctggtcaggg tggctagttt gtggtataat gaaagggact caaaaagaaa
     5461 caggagaaaa atgatggatt tacttttagc aattgtattg attgtgctag cttttctagg
     5521 aggagctctt ggaggaatgt acttggttcg taagcaaatc gaaaaagaat ttgctgacaa
     5581 cccacgtttg aatgctgaag cagttcgtac tcttttgagt gcaaatggtc aaaaaccaag
     5641 cgaagctaag gtacaacaag tttaccacca aatcatccgc caacaaaagg cagcccttgc
     5701 taacaataaa aagaaaaaat aaataaggaa aagtctggga tgaaagttcc agacttctca
     5761 ctatgttggt tatggtttag ggatgagact ttttccttgc attctattga tatttgatta
     5821 tgattaagag agaggtagtt gacattctat caagctgtcg gtcagttcgt tcagtacaag
     5881 gaatcataat gaacgatcaa tcagaaaaaa gactagaaag aagactgtat ggataatcga
     5941 ccaattggtt ttttggattc gggtgtcggg ggcttgaccg ttgtgcgcga gctcatgcgc
     6001 cagcttcccc atgaagaaat cgtctatatt ggagattcgg cgcgggcgcc ctatggcccc
     6061 cgtcctgctg agcaaattcg tgaatatact tggcagctgg tcaactttct cttgaccaag
     6121 gatgtcaaaa tgattgtcat tgcttgtaac actgcgactg cggtcgtctg ggaagaaatc
     6181 aaggctcaac tagatattcc tgtcttgggt gtaattttgc caggagcttc ggcagccatc
     6241 aagtccagtc aaggtgggaa aatcggagtg attggaacgc ccatgacggt acaatcagac
     6301 atataccgtc agaaaatcca tgatctggat cccgacttac aggtggagag cttggcctgt
     6361 cccaagtttg ctcccttggt tgagtcaggt gccctgtcaa ccagtgttac caagaaggtg
     6421 gtctatgaaa ccttgcgtcc cttggttgga aaggtggata gcctgatttt gggctgtact
     6481 cattatccac tccttcgccc tattatccaa aatgtgatgg ggccaaaggt tcagctcatc
     6541 gatagtgggg cagagtgcgt acgggatatc tcagtcttac tcaattattt tgaaatcaat
     6601 cgtggtcgcg atgctggacc actccatcac cgtttttaca caacagccag tagccaaagt
     6661 tttgcacaaa ttggtgaaga atggctggaa aaagagattc atgtggagca tgtagaatta
     6721 tgacaaataa aatttatgaa tataaggatg accaggactg gtatgttggg tcttatagta
     6781 tttttggtgg cgttaacagt ttgagcgact ataagacaga ttttcctctg tttgaattct
     6841 ccaaaatatt tggagatgaa gagtatggtt tcccgctttc agttactgtt ttacgctatg
     6901 gttctaccta ccgtttgttc tcctttgtgg tagacatgct taatcaagaa atgggacgaa
     6961 acttggaagt tattcaacgt catggggccc tgctcttggt tgaaaatggg caactcttgt
     7021 atgtagaatt gcctaaagaa ggggtcaatg ttcatgattt ctttgagaca agcaaggtca
     7081 gagaaacctt gttgattgcg actcgtaacg aaggtaaaac caaggaattc cgagctatct
     7141 ttgataagtt aggctacgat gtggaaaatc ttaatgacta ccctgacctg cctgaagtag
     7201 cagaaacagg tatgaccttt gaagaaaatg cccgccttaa ggcagaaacc atttctcaat
     7261 taacgggcaa gatggttttg gcagatgatt ctggtctcaa agtcgatgtc cttggtggct
     7321 taccaggcgt ctggtcagct cgtttcgcag gtgtgggagc aactgaccgt gaaaataatg
     7381 ccaaactctt gcacgaattg gccatggtct ttgaactcaa ggaccgctcg gctcagttcc
     7441 acacaaccct agtcgtagcc agcccaaata aggaaagttt agttgttgaa gcagactggt
     7501 caggttatat taactttgaa cctaagggtg aaaatggctt tggctatgat cccctcttcc
     7561 ttgtaggaga aacaggtgag tcatcagctg aattaaccct ggaagaaaaa aatagtcaat
     7621 ctcaccgtgc cttagccgtt aagaaacttt tggaggtatt tccatcatgg caaagcaaac
     7681 catcattgta atgagcgatt cccatggcga tagcttgatt gtggaagaag tccgtgatcg
     7741 ctatgtgggc aaagtcgatg ccgtttttca taacggcgat tctgaactac gtccggattc
     7801 tccactttgg gagggcatcc gcgttgttaa agggaacatg gacttctacg ccggctaccc
     7861 agaacgtctg gtgactgagc ttggttcgac caagattatc caaactcatg gtcacttgtt
     7921 tgacatcaat ttcaactttc aaaagttgga ctactgggct caggaggaag aggccgctat
     7981 ctgcctctat ggtcacttgc atgtgccaag tgcttggttg gaaggcaaga tcctctttct
     8041 aaatccaggt tctatcagtc aaccacgagg taccatcaga gaatgtctct atgctcgtgt
     8101 ggagattgat gatagttact tcaaagtgga ctttttgaca cgagatcacg aggtgtatcc
     8161 aggtttgtcc aaggagttta gccgatgatt gccaaggagt ttgagacttt cttgttgggg
     8221 caggaggaaa cttttttgac ccctgctaaa aatctagctg tgttgattga tacccacaat
     8281 gcggatcatg cgaccctctt gctcagtcag atgacctata cccgtgttcc cgttgtgaca
     8341 gatgaaaaac agtttgttgg gacgattgga ctcagagata ttatggctta tcagatggag
     8401 catgacttga gccaagaaat catggcggat acggatatcg ttcatatgac aaaaacggac
     8461 gtagcggttg tttcgcctga tttcaccatt acggaggtct tgcacaagct agtagatgag
     8521 tccttcttac cggttgtgga tgcagagggt attttccaag ggattattac gcgcaagtcc
     8581 atcctcaagg ccgttaatgc cctcttgcat gactttagta aggaatatga gattcgatgc
     8641 caatgagaga caggatttca gcctttttag aggaaaagca gggcttgtct gtcaattcca
     8701 agcagtccta taagtatgat ttggagcaat ttttagacat ggtaggtgag cggatttctg
     8761 agaccagtct caagatttac caagcccagc tagccaatct aaaaatcagc gtccagaagc
     8821 gaaagatttc ggcctgtaac caatttctat actttctcta tcaaaaagga gaggtggaca
     8881 gcttttaccg cttggaatta gccaaacaag ctgaaaagaa gacggaaaag ccagagattc
     8941 tatacctaga ctctttttgg caggaaagcg accatccaga gggccgcttg ctagcgctct
     9001 taatcctaga aatggggctc ttgcccagtg agattttagc catcaaggtt gcggacatca
     9061 atctggattt tcaggtgttg cgaatcagca aggcttccca acagaggatt gtcaccattc
     9121 ccacggcctt gctttcagaa ttggaaccct tgatggggca gacctatctt tttgaaagag
     9181 gagagaaacc ctattctcgt cagtgggcct ttcgtcagtt agaatctttt gtcaaggaga
     9241 aaggttttcc atccttatca gctcaagtct tacgtgaaca gtttattcta agacaaatag
     9301 aaaacaaggt cgatttgtac gaaattgcaa aaaaattagg attaaaaaca gtcctgacct
     9361 tagaaaaata tagataatgg atattaaatt aaaagatttt gaaggacccc tggacttgct
     9421 cttgcatctg gtttctaagt accagatgga tatctacgat gtgcccatta cggaagtcat
     9481 cgaacagtat ctagcctatg tctcaaccct gcaggccatg cgtctggaag tgacgggtga
     9541 gtacatggtc atggctagtc agctcatgct gattaagagt cgtaaactcc ttccgaaggt
     9601 agcagaagtg acagacttgg gggatgacct ggagcaggac ctcctctctc aaatcgaaga
     9661 atatcgcaag ttcaagctct tgggtgagca cttggaagcc aagcaccaag aacgggccca
     9721 gtattattcc aaagcgccga cagagttgat ttacgaagat gcggagcttg tgcatgacaa
     9781 gacgaccatt gacctctttt tggctttttc aaatatccta gccaagaaaa aagaggagtt
     9841 tgcacaaaat cacacgacga tcttgcggga tgagtataag attgaggaca tgatgattat
     9901 cgtgaaagag tccttgattg gacgagatca attgcgcttg caggatttgt tcaaggaagc
     9961 ccagaatgtc caagaggtca tcaccctctt tttggcaacc ctagagttaa tcaaaaccca
    10021 ggagctgatc ctcgtgcaag aggagagttt tggagatatc tatctcatgg aaaagaagga
    10081 agaaagtcaa gtgcctcaaa gctagacttg atagagagga aagatgagta ctttagcaaa
    10141 aatagaagcg ctcttgtttg tagcgggtga agatgggatt cgggtccgcc agttagctga
    10201 actcctctct ctgccaccga caggcatcca gcaaagttta ggaaaattag cccagaagta
    10261 tgaaaaggac ccagattcca gtttggcttt gattgagaca agtggtgctt atagattggt
    10321 gaccaagcct caatttgcag agattttgaa ggaatactct aaggcgccta tcaaccagag
    10381 cttgtctcgg gctgcccttg agaccttgtc cattattgcc tacaaacagc cgattacgcg
    10441 gatagaaatt gatgccatcc gtggggttaa ctcgagtgga gccttggcaa agttgcaggc
    10501 ttttgacctg ataaaggaag acgggaaaaa ggaagtattg gggcgcccca acctctatgt
    10561 gactacggat tatttcctag attacatggg gataaaccat ttagaagaat taccagtgat
    10621 tgatgagctt gagattcaag cccaagaaag ccaattattt ggtgaaagga tagaagaaga
    10681 tgagaatcaa taagtatatt gcccacgcag gtgtggccag taggagaaaa gcagaagagc
    10741 tgatcaagca aggtttggtg acggttaacg gccaagtggt gcgtgaacta gcaaccacta
    10801 tcaagtcagg cgacaaggtc gaagttgaag gtcaacctat ctacaacgaa gaaaaggtct
    10861 actatctgct taacaaacca cgcggtgtga tttccagtgt gacagatgat aagggtcgca
    10921 agacggttgt cgacctcttg cccaatgtca aagaacgtat ttaccctgtg ggtcgtttgg
    10981 actgggatac atcaggtgtc ttgattttga ctaatgatgg ggactttaca gacgagatga
    11041 ttcaccctcg taatgagatt gacaaggttt atgtcgcgcg tgttaaaggt gtggccaata
    11101 aggacaatct tcgccccttg acccgtggtc ttgagattga tggtaagaaa accaagccag
    11161 ctgtttatga aattctcaaa gtggacccag tcaaaaatcg ctctgtggtg cagttgacca
    11221 tccatgaagg gcgtaaccat caggttaaaa agatgtttga agctgttggt ctccaagtag
    11281 ataagttgtc tcggactcgt ttcggacacc tagacttgac aggactccgt ccaggagaat
    11341 cccgtcgtct taataaaaaa gaaatcagcc aactacacac catggctgta actaagaaat
    11401 aatgaaacga attttaatag cgcctgtgcg cttttaccaa cgttttatct caccagtctt
    11461 tccaccctct tgtcgctttg atctgacttg ttccaactac atgattcagg ctattgaaaa
    11521 acatggtttt aagggagtat tgatgggctt ggctcggatt ttacgttgtc atccctggtc
    11581 gaaaacaggt aaggaccccg ttccagacca cttttccctt aaacgaaatc aagaagggga
    11641 atgaggtggg gtaaatagat ttcaaaatga taaaaacgca tcctatcagg tttgagtgaa
    11701 cttgatagga tgcgttttag aatgtcaaaa ttttatactc ttcgaaaatc ccttcaaacc
    11761 gcgtcagctt tcatctgcaa cctcaaaaca gtgttttgag caacctgcgg ctagtttcct
    11821 agtttgctct ttgattttca ttgagtatta aattgagttt gaagtggctt atttcaaagc
    11881 tttttgtatg tcttcaatca tgagttttgt tgattcaagt ccgcctccgc ttagatacca
    11941 gaggtctggt gttagttgga taatcttacc atttttagca gcaggtgttt cagcgataag
    12001 ggcattttct aggacaccgt cgttgctaga gttgtcccca ccgatggcaa gggtacggtt
    12061 gatgacaaag aggatgtcag ggttgatttc tttgacactt tcaaagctga cttcttgtcc
    12121 gtggcgtgag tcttcaaatt ttgtatcagt tggtttgaat ttcaaggttt ggtacaagaa
    12181 agagaaacga gatttggcac caaaggctgc catttttcct tcattaagga ggatcgcaag
    12241 ggcttttttg tcagagcttt catttttagt agcgacttct tggatgctct tgtctagctt
    12301 ggccaattct tccttggctt tttgtgtact agtttcgccg aaggcacttg ctaaggattc
    12361 gatattagcc ttggtagaag tccagtagtc atccttgctt gcttggaaga gaacggttgg
    12421 ggcgatttct ttgaatttgt ctacgaattt ttgtgtacgt ggtgaagcga taatcaaatc
    12481 aggctcaagg gcggcgatag cttctaaatc aggttctttc atagaaccaa catttttgac
    12541 agttcccact aggtctttta gataagtcgg aacagttttt gtaggcattc cgacgatatt
    12601 tttttcaaat cctaaagcgc gaatagtatc tgcagcgccg aggtcaaagg tcacaatctt
    12661 ttcaggaact ttggaaagtt tgacctcgtc cagtgaactt ttaatggtta tctctgttgg
    12721 agcagagcta ctggtctctg tctgactagt gcttgagttt gtactacatg caccaagtag
    12781 gagcaagaag ctggccacta gggcagtgaa ataaagttta agggatgttt tcataatttc
    12841 tcctttttaa aatgtgataa cgatttaggg agtctcttaa tcttattgac taagagactg
    12901 aaggttctct aacttgagct tttatgttac tagctataga tacagatctt tttgttattg
    12961 atatcagcta gcgtgatggg aatctcataa agttgactga gcaggtcagc ctgcatgatt
    13021 tgatcggttc ttcccttgct aaagacctgg ccgtccttga aggcgacaat ttcatctgca
    13081 tactgactgg ccatgttgat atcgtggagg acgatgataa tggtcttgcc gagttcctcc
    13141 accagtcgtc gaagaatctg catcatgctg acgctttgct tgatatcgag attgttgagt
    13201 ggttcgtcca gcaagataaa gtccgtatcc tgggccagta ccatagcgat aaagacgcgc
    13261 tggagttgcc cccctgacag gctattgatg tagcggtctt ttaagttggt cagttccaaa
    13321 tagttcagag tttctcggat tttttcccag tcttctgatc taagtcgacc tcggctgtag
    13381 ggaaaacgtc caaaactgac cagttcttca acagtcaatt tggcttggta attgattttc
    13441 tgttttagga tggttagttc ttgggccagt tcttgcgaat tccagctctc gatttcacgt
    13501 cctttgatac tgagaactcc ctgatctttc ttggttagtc tgctcatgat ggagaggaga
    13561 gtcgattttc cagcaccatt tggaccaata aaggctgtca gtttttgagg actgacttca
    13621 agcgaaatgc cttgcaaaat atcctgtttt tgaatggatt tgtcaatgtt ttccagtttc
    13681 actgacgaga cctcctatat agtaagataa agaataagaa gccacccaca ctctcaatga
    13741 tcatactgat acgaatttcc agtgcaaaga ctcgttcaat caaggcttgc cccaaggtta
    13801 agctaataaa tccaaccaga atggccacta taaagagtaa cttgtgctga tagtctttga
    13861 caatcaggta ggtgaggttg gccagcataa agccgaagaa ggccataggt cctaccaagg
    13921 cagtggccgt tgaggtcaaa agcacgattc cccagaggag ctctttctgt tctttttcaa
    13981 catcgagtcc caatatctga gccgtttctc tttgcaggtg caagacatct agaacgactg
    14041 cttttcgaaa gaaaaagatt gtcaaagcga ggatgatcag agaaccgatg gctaggatgg
    14101 aagtgttgag atgttgaaag gaggcaaaaa gactattttg cagtttatcg tattcgtttg
    14161 gatccattag gacttgaagg aaggtgctga tatttcgaaa gagacttctg agcgctagac
    14221 agatcagcag gatgaagacc aggtcttgct tcatcagtgt cttcaagtaa ccttgtaagg
    14281 cgagaaagaa gagggactgg acaagaagta agactaggaa ttctaagata ggggatttgc
    14341 caagttgaag aaacttgctt tcaaaaacca gtagtagggt ttgtagtagg acgtagaagg
    14401 attcaattcc caaaatacta ggcgtcagga agcgattttc cgtcagggtt tgaaaactaa
    14461 tggtcgaaat cccagtcgcg atggctacca agagataaac gatgatcttt tgggaacgca
    14521 acttccaagc aaaggctgac aagtgagtga tgggccaaaa gtagagaaga caagctccga
    14581 tggcaagaat aatgagaatc cagaagagct tggtatgttt gcttttagtc tgcatctttt
    14641 cgtccccctc tccagagaag taggataaag acgagactac cgatgattcc tagcaagaga
    14701 ctgacagaca actcataggg tctaatcaga actcgggata ggatatcgca agccagaact
    14761 agattggcac caaccagtgc gaccatgagt ttggtttgac ttagattatc tccatagcgc
    14821 ttgcgaacaa gattgggaac gataactccg cgaaatggta agccacccac ggtaatcatg
    14881 gtgacgcttg tcgttagcgc caccagaaag agggccagtt tttcaagtag ggagtaggaa
    14941 atccccaaac tctcgctggt ttctttccct agattcatga tggtgaaggt ttgggataat
    15001 ttccaaacgg ttatcaggat gatgaggcct aagaagagcc actcatactg atgggtctga
    15061 atcatggaga aggagccctg ggtccaggca gtcatactct gaaccagatt gaaacgatag
    15121 gcgataactt ctgtgactga gccgataatc ccgctataga tgatcccaat cagaggcaac
    15181 atccaccttt cctttacagt aaaaatggtc ataaaggcta ggaagaagag ggtgaatacg
    15241 atggatgaaa caaaagcgaa gagcatcttt tgggtcagac tagccgatgg aaagacaaaa
    15301 aggctcagca ccattcccag tttggcggct tcagtcgttc caactgtact cggtgcagca
    15361 aactgatttt gggtaatagt ctgcatgaga aggcctgcca tactcatact agaggcagtc
    15421 aggagaatac tgatagttct tgggagacgg gactcttgaa agaggagcca ggtctgctgg
    15481 tcgaaatcaa atagctttcc ccatgaaaaa tcactggtcc caatgctaat agagagaaag
    15541 actaggagta gaagtaagcc aattaaataa tgagaaagtt tcataccccg tcctttcatg
    15601 tagatttggt atcgaaagat acctgcggat ataaatgtaa cattattttt ctaatctgtc
    15661 aataaaattt ctgacaattt aataaataca acaaggagag agcaacaaga ctttctcctt
    15721 tgttatccta ttctaaaatg tttttacctt aatctgataa aataatatct tcgagggagt
    15781 agctagccgt ccaatcaaga tattgtttag cttttgaagc atctgctagg acactggctg
    15841 ggtcactagc acgtcgagca acaatctcgt gtgggatttt ttaatttagt aattcttcag
    15901 cagttttaaa gatttctttg atagtatagc cttttttagt tcctaagtta aagatttgag
    15961 aagaactgtc ttcttgaaat aggtagttca ttcctttaac atgagcctat gcaaggtcca
    16021 agacataaat gtaatctcga atacatgaac cgtcacgtgt atcgtagtca tctccaaata
    16081 tttttaagct atcattttgt cccaatgcgg tcttgttgat atttggaatg atgtgagttg
    16141 gatttttcac acgcagaccg tttgaagcat ccatttcagc cccagcaaca ttaaagtaac
    16201 ggaaaataac atatttccag tcgtagcgat tggccatcca gtaaatcatt cgttcgccca
    16261 tcagttttgt ctctgcataa gggttgacag ggtcgagcag ggtatcttca gtcaccggct
    16321 tgtcaataca gttatttcca tagagagaaa cagtcgaaga gaacatgatt ttttgaatgc
    16381 caacttcaga taagactttg agaacttggt ccataccagc aacgttggca gtgaagtatt
    16441 tacttggatt ttcaatactt tcgctcacaa caatttcacc tgcataatgg aggacagcat
    16501 caatctgatt ttcttctaag taagccttta aggcgctagc atcataaaca tccagttctt
    16561 taaagctagc acgactaccc acagccgctc gatttcctgt ggagagatta tcgaggacat
    16621 gcaccttata accagcattt aaaagagctt taacggtatg ggagccaatg tagctagtcc
    16681 cacctgtaac aagaattgtt ttggtcatga ttttttcctt ttctagtttt gtactatagt
    16741 a
//
LOCUS       DAVKWZ010000043        16446 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000043 DAVKWZ010000000
VERSION     DAVKWZ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16446
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.043"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            <1..274
                     /locus_tag="VW854_001503"
     CDS             <1..274
                     /locus_tag="VW854_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262721.1"
                     /translation="DNHVLTLIFLIKICVDTTIKFLFIRLFDTSTLMKRRIFFLFALD
                     VIAYLFLGYLLVIQKAGYLFSVLLLFSNFSVPFIKEKEYELFKNSK"
     gene            1413..1652
                     /locus_tag="VW854_001504"
     CDS             1413..1652
                     /locus_tag="VW854_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262722.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     DLYKITRDYASQTKPAIQAGVFILEDFRNILCTMM"
     gene            1637..1915
                     /locus_tag="VW854_001505"
     CDS             1637..1915
                     /locus_tag="VW854_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262723.1"
                     /translation="MYNDVIERISLYEFIGDIFYSKIISCCIVARDLSKNTMKLDVIF
                     FEDKNKRSAVLGLRRDKSGVFKSVILHFTSAKKYAKVRKTDVKEMKWL"
     gene            2201..3997
                     /gene="pspA"
                     /locus_tag="VW854_001506"
     CDS             2201..3997
                     /gene="pspA"
                     /locus_tag="VW854_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein A"
                     /protein_id="HEV0262724.1"
                     /translation="MNKKKMILTSLASVAILGAGFVASQPTVVRAEEAPVASQSKAEK
                     DYDAAKKDAKNAKKAVEDAQKALDDAKAAQKKYDEDQKKTEEKAALEKAASEEMDKAV
                     AAVQQAYLAYQQATDKAAKDAADKMIDEAKKREEEAKTKFNTVRAMVVPEPEQLAETK
                     KKSEEAKQKAPELTKKLEEAKAKLEEAEKKATEAKQKVDAEKYALEAKIAELEYEVQR
                     LEKEIKEIDESDSEDYLKEGLRAPLQSELDTKKAKLSKLEELSDKIDELDAEIAKLEK
                     DVEDFKNSDGEQAEQYLVAAEKDLDAKKTELEKTEADLKKVANEPETPAPAPKPETPA
                     PAPEAPAPEAPAPAPEAPAPAPAPKPEQPAPAPKPEKSADQQAEEDYARRSEEEYNRL
                     TQQQPPKAEKPAPAPAPKPEQPAPAPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWY
                     YLNANGSMATGWVKDGDTWYYLEASGVMKASQWFKVSDKWYYVNSNGAMATGWLQYNG
                     SWYYLNANGAMATGWAKVNGSWYYLNANGSMATGWVKDGDTWYYLEASGAMKASQWFK
                     VSDKWYYVNGLGALAVNTTVDGYEVNANGEWV"
     gene            4398..5519
                     /gene="mnmA"
                     /locus_tag="VW854_001507"
     CDS             4398..5519
                     /gene="mnmA"
                     /locus_tag="VW854_001507"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEV0262725.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRRLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            5660..6115
                     /locus_tag="VW854_001508"
     CDS             5660..6115
                     /locus_tag="VW854_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV0262726.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYTIGGA
                     IQVNEKTEDAVVREVREELGVKAQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKSQPCEWIDLDKLEDIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            6125..8038
                     /gene="mnmG"
                     /locus_tag="VW854_001509"
     CDS             6125..8038
                     /gene="mnmG"
                     /locus_tag="VW854_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEV0262727.1"
                     /translation="MIYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKAVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEVPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEELDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            complement(8233..9912)
                     /locus_tag="VW854_001510"
     CDS             complement(8233..9912)
                     /locus_tag="VW854_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV0262728.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(9914..10147)
                     /locus_tag="VW854_001511"
     CDS             complement(9914..10147)
                     /locus_tag="VW854_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEV0262729.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDTSSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            complement(10965..11117)
                     /gene="cibB"
                     /locus_tag="VW854_001512"
     CDS             complement(10965..11117)
                     /gene="cibB"
                     /locus_tag="VW854_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEV0262730.1"
                     /translation="MMKNLNNYREISNKELQEIKGGFGVGVGIALFMAGYAIGKDLRK
                     KFGKSC"
     gene            complement(11119..11304)
                     /gene="cibA"
                     /locus_tag="VW854_001513"
     CDS             complement(11119..11304)
                     /gene="cibA"
                     /locus_tag="VW854_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEV0262731.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            11482..12165
                     /gene="tsaB"
                     /locus_tag="VW854_001514"
     CDS             11482..12165
                     /gene="tsaB"
                     /locus_tag="VW854_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEV0262732.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQILAEMTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAHTLNIELVGMSSLLALVPY
                     QQEGLFVSLMDARRNNVYAGFYENAKPVMPEAHLSFEEVLEKVKGASQVTFVGEVGPF
                     VEQIQEYLPRTNYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            12162..12599
                     /gene="rimI"
                     /locus_tag="VW854_001515"
     CDS             12162..12599
                     /gene="rimI"
                     /locus_tag="VW854_001515"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEV0262733.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVMAAVYLVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RQSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            12589..13599
                     /gene="tsaD"
                     /locus_tag="VW854_001516"
     CDS             12589..13599
                     /gene="tsaD"
                     /locus_tag="VW854_001516"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEV0262734.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFANLDLNAKPSLAFDT
                     ME"
     gene            complement(13639..14945)
                     /locus_tag="VW854_001517"
                     /pseudo
     CDS             complement(13639..14945)
                     /locus_tag="VW854_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(15278..15572)
                     /locus_tag="VW854_001518"
                     /pseudo
     CDS             complement(15278..15572)
                     /locus_tag="VW854_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(15679..15972)
                     /locus_tag="VW854_001519"
                     /pseudo
     CDS             complement(15679..15972)
                     /locus_tag="VW854_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         4968 a         3083 c         3706 g         4689 t
ORIGIN      
        1 tgataatcat gtactgactc ttatcttttt gataaaaatt tgtgtagata ccacgataaa
       61 atttttattc atcagattat ttgacacaag tactttaatg aagaggagaa tattttttct
      121 tttcgcattg gatgttatag cttacttatt tttaggatat cttttagtga ttcaaaaagc
      181 aggctatttg ttttcggttt tacttctttt ttctaatttt tcagttccat ttatcaaaga
      241 aaaggaatac gaattattta aaaatagcaa gtaatagaaa cataggttaa atcttagaac
      301 ggcttaaaat cagatatgat aaagggaaat agtcttatct aggtaactaa gtgctacaaa
      361 cacagtcgag gatcacttgt taggattaca ttttaatcat gcttaataag aatggagaat
      421 tcctatgggg attgtattac tctgaagttt cgaatacctt agttctcgtg attttgagct
      481 attctagctt tagaatcctt cagaatgtaa aattttaagc agtcaaagac ttagaatagc
      541 ctgaaaacat ttattttata gcgattctaa gcttagaatt gattggatat atatgtattt
      601 gagctgctcg atagttagga cagctctatt ctgagaaatc tgagctatca atgcttcaga
      661 atagtatttc gccaatggtt tttattgaag aggtgattgt tttatgatag tctaaaataa
      721 aactgattta atggagtaaa tggagaatcg ttacatgaaa catttttttg taggaattgg
      781 tgagataaaa cttgttggct acttattgta tatatgtgta ggactagccc ctctttttca
      841 tatatatgtc ataggttcgg aactgagttt tgtgaaaata gtgttatcta ttttgggagt
      901 tatatttgta agtatgttaa ctattgctcg aatttatagg acttttttct ataaagaata
      961 gtttggactt gaactgtatc taaaaacaga ggttggtcga ttatcagcct ctttcttgtc
     1021 gttgaaaaga gtgagatata ttgacttttg aaaaaaagat gtcataattg agttgggata
     1081 aacctaaaag taatatagta gattgaatct ggaatagtcc gttatgactg ttaaaatatt
     1141 tctagaaatt aatttgactt tcctaatcga tttgttcaca tcttgtttca atctactata
     1201 ctttctcgtt aaaccgttcg gcaattcatg gtgacatgga cacatagtag taagcacact
     1261 atgtggcttg gcagagctaa aaactgttcc cttgcgataa gcctaataag cataacatag
     1321 ggagttagag aagccgactc taatcaacca ctttgggcag tagtgagaac tgccccgtgc
     1381 ttttttattt tgagaaaata tggagtttgt cgttgaaatt acttgattgt attttggact
     1441 atcaagaaag attcaatgga aaaacatgtc aagtatcaac gaattataag tatttagaga
     1501 ttttcaaagt gaatttttgc ttgactgatt tacatcattt atttgactta tacaaaatca
     1561 cacgagatta tgctagtcaa acaaaacctg ctattcaagc tggtgttttt attttagaag
     1621 attttagaaa tatcctatgt acaatgatgt aatcgaaagg atatctttat atgagtttat
     1681 cggtgatatt ttctattcta aaataataag ttgttgcatc gtagctaggg atttatctaa
     1741 aaatactatg aaattggacg tcatattttt tgaggataaa aataaaagat ccgcagtttt
     1801 aggtttacga agagacaaaa gcggagtatt taaatcagtt attctacatt ttacaagcgc
     1861 taagaaatat gctaaagttc gtaaaacaga tgtgaaagaa atgaaatggc tataaagatt
     1921 gtccgcaggc ttaagcttgc gttttttgtt gtttaacgaa attaaaaaac aagctctctc
     1981 atcggaaatg tttttatagg aatgaaggaa gatgatgcta tgtttaaaaa gagaatcgaa
     2041 aagggcaaaa gtagtgtctt tatctttttg gagtagaagc ttatgatata gaaatttgta
     2101 ataaaaatgt aatataaaac atttgacaaa tatttacggg gggagtatac ttaatataag
     2161 tatagtctaa aaatgactat cagaaaagag gtaaatttag atgaataaga aaaaaatgat
     2221 tttaacaagt ctagccagcg tcgctatctt aggggctggt tttgttgcgt ctcagcctac
     2281 tgttgtaaga gcagaagaag ctcccgtagc cagtcagtct aaagctgaga aagactatga
     2341 tgcagcgaag aaagatgcta agaatgcgaa aaaagcagta gaagatgctc aaaaggcttt
     2401 agatgatgca aaagctgctc agaaaaaata tgacgaggat cagaagaaaa ctgaggagaa
     2461 agccgcgcta gaaaaagcag cgtctgaaga gatggataag gcagtggcag cagttcaaca
     2521 agcgtatcta gcctatcaac aagctacaga caaagccgca aaagacgcag cagataagat
     2581 gatagatgaa gctaagaaac gcgaagaaga ggcaaaaact aaatttaata ctgttcgagc
     2641 aatggtagtt cctgagccag agcagttggc tgagactaag aaaaaatcag aagaagctaa
     2701 acaaaaagca ccagaactta ctaaaaaact agaagaagct aaagcaaaat tagaagaggc
     2761 tgagaaaaaa gctactgaag ccaaacaaaa agtggatgca gaaaaatatg ctcttgaagc
     2821 taaaatcgct gagttggaat atgaagttca gagactagaa aaagagatca aagagattga
     2881 tgagtctgac tcagaagatt atcttaaaga aggcctccgt gctcctcttc aatctgaatt
     2941 ggataccaaa aaagctaaac tatcaaaact tgaagagttg agtgataaga ttgatgagtt
     3001 agacgctgaa attgcaaaac ttgaaaaaga tgtagaagat ttcaaaaact cagacggtga
     3061 gcaagctgaa caatatcttg ttgcagctga aaaagactta gatgctaaaa aaactgaatt
     3121 agaaaaaact gaagcggacc ttaagaaagt agctaatgag ccagaaactc cagccccagc
     3181 tcctaaacca gaaactccag ccccagctcc agaagctccg gctccagaag ctccggctcc
     3241 agctccagaa gctccggctc cagctcctgc accaaaacca gagcaaccag ctccagcacc
     3301 aaaaccagag aagtcagcag atcaacaagc tgaagaagac tatgctcgta gatcagaaga
     3361 agaatataac cgcttgactc aacagcaacc gccaaaagca gaaaaaccag ctccagctcc
     3421 tgcaccaaaa ccagagcaac cagctcccgc accaaaaacg ggctggaaac aagaaaacgg
     3481 tatgtggtac ttctacaata ctgatggttc aatggctaca ggttggctac aaaacaacgg
     3541 ttcatggtac tacctcaacg ctaacggttc aatggcaaca ggttgggtga aagatggaga
     3601 tacctggtac tatcttgaag catcaggtgt tatgaaagca agccaatggt tcaaagtatc
     3661 agataaatgg tactatgtca acagcaatgg cgctatggcg acaggatggc tccaatacaa
     3721 tggttcatgg tactacctca acgctaacgg tgctatggca acaggttggg ctaaagtcaa
     3781 cggttcatgg tactacctca acgctaacgg ttcaatggca acaggttggg tgaaagatgg
     3841 agatacctgg tactatcttg aagcatcagg tgctatgaaa gcaagccaat ggttcaaagt
     3901 atcagataaa tggtactatg tcaatggctt aggtgccctt gcagtcaaca caactgtaga
     3961 tggctatgaa gtcaatgcca atggtgaatg ggtttaagcc gattaaatta aatcatgtta
     4021 agaacatttg acattttaat tttgaaacaa agataaggtt cgattgaata gatttatgtt
     4081 cgtattcttt aggtacctgt cttatgattt caggaaatgt cattaaaaaa acgactcctt
     4141 ttctctaact tgaaaaatag attagagaaa atgggttgtt ttatttatta tagttatttg
     4201 aatgaagata agaagaaggt atactcacat cattcacata atctgtatgt tgactataag
     4261 tttaaaaaaa caatttttaa gctcttcctt gtcttctcca accaagcgtg ttataatgaa
     4321 tactgctcaa gcgaccttca atcgtgaagc acacacgacc ttcaatcgtg aataaacgaa
     4381 tagatgggag acttaccatg agtgataact ctaaaacacg tgttgtcgtg gggatgagtg
     4441 gtggtgttga ttcgtcggtg acggctcttt tgctcaagga gcagggctac gatgtgatcg
     4501 gtatcttcat gaagaactgg gatgacacag atgaaaacgg cgtctgtacg gcgaccgaag
     4561 attacaagga tgtggttgcg gtggcagacc agattggcat tccctactac tctgtcaatt
     4621 ttgaaaaaga gtactgggac cgcgtttttg agtatttcct agcggaatac cgtgcagggc
     4681 gcacgccaaa tccggacgtt atgtgcaaca aggaaatcaa gttcaaggcc tttttggact
     4741 atgccatgac cttgggggca gactatgtag cgactgggca ttatgctcga gtggcgcgtg
     4801 atgaggacgg cactgttcac atgcttcgtg gcgtggacaa tggcaaggac cagacctatt
     4861 tcctcagcca actttcgcaa gaacaactcc aaaaaaccat gttccctctg ggacatttgg
     4921 aaaagcctga agtacgcaga ctagcagaag aagcaggcct ttcgactgct aagaagaaag
     4981 actcgacagg gatttgcttt atcggagaaa agaactttaa aaactttctc agcaactacc
     5041 tgccagctca gcctggtcgc atgatgactg tggatggtcg cgatatgggc gagcatgcag
     5101 gtcttatgta ctatacaatc ggtcagcgtg gcggactcgg tatcggtggg caacacggcg
     5161 gtgacaatgc cccttggttc gttgtcggaa aagatctaag caagaatatt ctctatgtag
     5221 gacaaggatt ctaccatgat tcgctcatgt caactagcct agaagccagt caagtccact
     5281 ttactcgtga aatgccagaa gagtttacgc tagaatgtac ggctaaattc cgttaccgtc
     5341 agcctgactc taaggtgacc gtccatgtca aaggagacaa ggcagaggtc atctttgcgg
     5401 aaccacaacg cgcgattaca ccaggacagg cagttgtctt ttacgatggc gaagagtgtc
     5461 taggtggtgg tttgattgac aatgcttacc gcgatggaca agtttgtcag tacatttaga
     5521 ttgacaaatt ttctcaattt gctacaataa taacagcaat agaaatgatg gtcaaagctc
     5581 atggatgttg caggcttttt tgtcctgcac ttctttgtag ttttgactgt ttttgtgtcg
     5641 tttaagggaa aggacaagaa tgactcaaca agactttcgg acaaaagtag acaatacggt
     5701 ttttggtgtt cgggcgacag ccttgattgt ccaaaatcac aagcttctag ttaccaaaga
     5761 caagggcaag tattacacta ttggaggtgc gattcaagtt aacgaaaaaa cggaagacgc
     5821 ggtagtccgt gaagtgaggg aagaactggg tgttaaagct caagctgggc agctagcttt
     5881 tgtggttgaa aatcgttttg aagtggacgg cgtttcctat cacaatatcg agtttcatta
     5941 tctggtggat ttgcttgaag atgccccgtt gaccatgcag gaagatgaga aaagtcagcc
     6001 ctgtgagtgg attgacttgg ataagcttga ggatatccag ctagttccag cctttttaaa
     6061 aacagcccta ccagattggg aaggccaact aagacacatt catcttgagg aataggagag
     6121 aaacatgatt tatcatttta ctgaagaata cgatattatt gtaattggtg cgggacacgc
     6181 tggggttgag gcttccttgg ccgctagccg tatgggctgt aaggtcctgc ttgcgaccat
     6241 caatattgaa atgctggctt tcatgccttg taatccctct atcggtggtt ctgccaaggg
     6301 gattgtcgta cgtgaagtcg atgccctcgg tggcgagatg gccaagacta ttgacaagac
     6361 ttacatccag atgaagatgc tcaacacagg gaaggggcca gctgtccgtg ctcttcgtgc
     6421 gcaggctgac aaggaacttt actctaagga gatgcgcaag acagttgaaa atcaagaaaa
     6481 tctgaccctt cgtcaaacca tgattgatga gattttggtg gaagatggca aggctgtcgg
     6541 tgtgcgtaca gccacccatc aagaatatgc tgctaaggct gttattgtga cgacagggac
     6601 tgctctccgt ggggaaatta tcatcggaga cctcaagtac tcatcaggtc ctaaccacag
     6661 cttggcttct attaacctag ctgacaatct caaggaactg ggtctcgaaa tcggtcgttt
     6721 caagacagga acccctccac gtgtcaaggc ttcttctatc aattacgatg tgacagaaat
     6781 tcagccagga gacgaagtgc ctaatcattt ctcatacact tcacgtgatg aggattatgt
     6841 caaggaccaa gtaccatgct ggttgaccta taccaatggt accagtcatg agattatcca
     6901 aaacaacctc caccgtgcgc ctatgtttac aggtgtggtc aagggagtgg gacctcgtta
     6961 ctgtccatcg attgaggaca agattgtgcg ctttgcggac aaggaacgtc accaactctt
     7021 ccttgagcca gaaggacgca atacagagga agtctatgtt caaggacttt caaccagtct
     7081 gcctgaggat gtccaacgtg acttggttca ttccatcaaa ggtctggaaa atgcagagat
     7141 gatgcgaaca ggttatgcta ttgagtatga tatggtcttg cctcatcagt tgcgtgcgac
     7201 tttggaaacc aagaaaatct caggtctctt cactgctggt cagacaaatg gaacgtcagg
     7261 ttacgaagag gcagcaggcc aagggattat cgcgggtatc aatgcggctc tgaaaatcca
     7321 aggcaagcct gaattgattc taaaacgaag tgacggttac attggggtca tgatcgacga
     7381 cttggtgacc aagggaacca ttgaacctta ccgtctcttg accagtcgtg ctgaatatcg
     7441 tctcattctt cgccatgaca atgctgatat gcgcttgact gagatgggcc gtgagattgg
     7501 ccttgtggac gatgaacgct gggctcgttt tgaaatcaag aaaaatcaat ttgataatga
     7561 gatgaagcgt ctagacagta tcaaactcaa gccagtcaag gaaaccaatg ctaaggttga
     7621 ggaaatgggc ttcaagccgt tgacagatgc ggtgacagcc aaggaattcc ttcgccgtcc
     7681 agaagtttct taccaagatg tggtggcctt catcggacca gctgcagagg aattagatga
     7741 caagattatc gaattgattg aaacagaaat caagtacgaa ggttacatct caaaagccat
     7801 ggatcaggtt gctaagatga agcgcatgga agaaaaacgt attccagcca atatcgactg
     7861 ggatgacatt gattctatcg caaccgaagc ccgtcagaag ttcaaactca tcaatccaga
     7921 aaccatcggc caagccagcc gtatttcggg agtaaaccca gcagatattt ctattttgat
     7981 ggtgtatctg gaaggtaaaa atcgtagtat ttctaaaact cttcaaaaat caaaatgata
     8041 cgtcgtaggc ttcttacgaa tgagttcaaa gcttggcttt gattcatctc caacctccca
     8101 tagttccccg aactatggga gctaactcaa gttatgcatt ttcctagcct tctggtctga
     8161 ttttcattga gttttaatct caaaagaaag catagagaaa aacagctccg aagacggggc
     8221 tgttttgttg ctttattctt catctggtgt gaggatcatc gggatgatga tcggttcacg
     8281 ttcggtattt tcatagagga aggggcgaat agcgttgaca atggcaccat tgacagattg
     8341 cacgctagca tccttatttt tcagtgcgat acgaatggca ttgaagagga tacgctggct
     8401 ttggcgaatc aagtctccag actctctcat gtagacaaag cctcggctga ggatatctgg
     8461 gccagacaga atcatctgcg atttgaagtc aacagttgcg actgccagaa cgacaccgtc
     8521 ttcagataga tcgcgacgat ctttgaggac agctgcgcca atttcaccga tacgatttcc
     8581 atcgacatag atatcttggg cgttgaaatg acctgcgata cgagctgagt cagcagtaag
     8641 ggcaagcaca tcgccattgc tcatgataaa gatattgtcc ttctcaacac cagtatccac
     8701 tgctagtcca gcgtggactt tttgcatgcg gtattcaccg tggacaggca tgaagtattt
     8761 tggcttaatc aagcagagca tgagtttttg ctcttgctga ccaccgtgtc cagatgtatg
     8821 gatattgttc actttaccgt ggataacttc gacaccagct tcagaaatga tgttaatcag
     8881 cttgttgacg ctagtagtgt ttccagggat gggactagaa gagaagataa cggtatcacc
     8941 tggttgtaat tgtacttgac ggtgggttcc gttggcgata cgagagaggg ctgccatagg
     9001 ctcaccctga ctacctgtac agaggataag aacttctcct gcaggataat ctttgatttc
     9061 atttggctcg ataaaggttc ccttaggagc tttgatgtag ccaagatcga ttccgttgac
     9121 aatggccttt tccatagaac gaccaaagac cgcaatcttg cgtccagtct taacagcagc
     9181 ttctgttgcc tgctggagac ggaagatatt tgaggcaaag gatgcaaaga tgatacgtcc
     9241 ttcaatacct tggataatct tcataatgga ctgaccaacg actttttcag agttggtaaa
     9301 ggttggtact tccgcatttg tcgagtcaga caggagacag agcacgcctt cttcaccaag
     9361 cgcagccata cgatgcaagt ccgcaggttc tccaactgga gtaaagtcga acttaaagtc
     9421 acccgtacag acgattttcc cttgaggagt atgaatgaca atccccaaag gctctggaat
     9481 agagtgagtc gttctaaaga aagttgcctt gagattttta aaggtcaact cggtgttgtg
     9541 gttgatttcg taaagtttgg cgttgcgcaa gaggccgtgt tcttcgagtt tcccacggat
     9601 caaagccaag gcaagcggtc cagcataaat agggacattt gcttgcttga gtaggaacgg
     9661 aatcccacca atgtggtcct cgtgtccgtg tgtgattaaa acagccttga cgcggtcgat
     9721 attgtctacg atgtaagagt agtcaggaat gacatagtcg ataccaagca agtcatcttc
     9781 tgggaattta atcccagcat cgacgatgat aatctcgtct tggtattcaa ttccgtaagt
     9841 gtttttcccg atttctccta gaccaccgat ggcaaaaaca ccaacttctt caggtttaag
     9901 agtgtaggcc atattagaac tccgtaattt cgaaggcgcc agtttctttt tcgtaatcga
     9961 gcaatttgtc agacaagagt tcgatatatt cgatattgta ctctgggcga ttttcttcga
    10021 caagttggcg agcagtgata cggccctcaa gttctgagct ggtatcgatg tctaggtaaa
    10081 gcgcgcgtgt tgtttcacgg cgtgggctac gttcttttgt ttcttgataa aaaactttgt
    10141 aaatcatata gttcctttct atttacaggt cagcttattc caaactttta gcagatattt
    10201 gcttgatttc attccatttt gtgtctagat tgaccttgat tcgttacaat tatttcaatt
    10261 ataccacaaa atgaaaaatt agtaaaagac ggaaacgaga aagttagggg acgatttctt
    10321 tggtgagcgt ttgcaaaaat aaagaaaatg tattatgttg ataacggaag tgaaatttat
    10381 tgcttgtcat aattccatat agattttaag tcaaggaggt tgccctatgt ataacttatt
    10441 tgcttttgct atcggagttc gtttgttagc ttttatcgtt tttgttgctt gcgtttatgg
    10501 aggaaatctt ctatttgcca aactggaacg atgacaaatc aagttcttga ggaaaattac
    10561 ctttgtgact ctctactcgc tttttctcct cctcgtgacc tatgtcgttt ggtttgtatt
    10621 ttactttgga ttaaatgctt agatgccctg cttatgctgg ggttttttgt ttggggatgt
    10681 agagatagta gatttacgac acgaaaattc caccccatct atcaagatga ggtggtcttt
    10741 gcatttttct aaaatgacat gacaatggaa ttttcaaagc ttattatttt tatgggagtc
    10801 attgttctgc tcaaaaatag agttgaagca taaaaataaa acaatacaaa cagataaatc
    10861 gaatggtaaa gggatagtag taatgttcaa atcattgaga atgctgagca aactgtaaga
    10921 gatgaagaca ataaaattta tccttctaaa aatgtgtttc ttatctagca tgatttacca
    10981 aactttttac gaaggtcttt tccaatggca taacctgcca taaataaagc gataccaaca
    11041 cctacaccaa agccaccctt gatttcttgc aattccttat tagaaatttc acgatagttg
    11101 ttcaaatttt tcatcatatt aatctcctcc taaaaaataa ccagctgcca aaccagaacc
    11161 tataagtgct gttccacctg caatagcttt ccaagatact gctactccaa agataaccaa
    11221 gggagcgaga ccgccatcaa tatctgataa ttcattttca gataaggata aaaattgatt
    11281 gtcaagaatg tcaaaatttg tcattgaact ttcctccttt tttcttgttc acttttatat
    11341 tcggaaaaga aaaggaaaag tgagcatttt tttaaattat ttttagttaa gtgagggcac
    11401 tcttgtctgg gggcaaagaa aattcccatg tgccagcttt tgtagtataa tagtaagcag
    11461 acaaaaagat aggaatgtgt tatgaaagta ttagcttttg atacgtccag caaggctctt
    11521 tctctcgcta ttttagagga caagcagatt cttgccgaga tgacgattaa tattaagaaa
    11581 aatcacagta ttaccctcat gcctgccatc gattttttga tggcaagttt ggactggaca
    11641 cccaaggatt tggaccgaat cgtggtggct gaagggccag gtagctacac aggcttgcga
    11701 attgcggtag ccactgccaa gactttggct catactctga acatcgagtt agttggtatg
    11761 tctagcctct tggctctggt accctaccaa caagaaggct tgtttgtctc cttgatggat
    11821 gcgcgtcgca acaatgttta tgcaggattt tatgaaaatg ctaaacctgt catgccagaa
    11881 gcgcacctat cttttgaaga ggtgctagaa aaagtcaagg gtgctagtca ggtaaccttt
    11941 gtcggagaag ttggcccctt tgttgagcag attcaagaat acttgccaag gactaattat
    12001 aaagaaacct tgcctaatgc agccaatctt gctcttttag cttgggacaa ggaagcagac
    12061 tccttgcatg attttgtgcc gaattacctc aaacgagtcg aggctgagga aaactggctc
    12121 aagaaccata ccgagtctgg cgagtcttac attaaacgcc tatgatagaa atcaagcgaa
    12181 ttcaacaaca gcctgaccta gctcaagcca tctacgctgt tatggcagct gtttacctag
    12241 tcagtccttg gactctggag caaatccaag cagatctgtc ccaagaccag acttggtatg
    12301 cattggctta tgatggggca gaagtgattg gatttctagc tgtgcaggag aatctttttg
    12361 aagcagaagt cctgcaaatc gctgtcaaag gagcttatca gggtcaggga attgcgtcag
    12421 ccttgtttgc tcaattgccg acagacaagg aaattttcct cgaagtcaga cagtcaaatc
    12481 aacgagcgca agcattttac aagaaagaaa agatggcagt tatcgctgag cgaaaggcct
    12541 actaccatga cccagtcgag gacgccatta tcatgaagag agaaatagat gaaggataga
    12601 tatattttag catttgagac atcctgtgat gagaccagtg tcgccgtctt gaaaaacgac
    12661 gatgagctct tgtccaatgt cattgctagt caaattgaga gtcacaaacg ttttggtggc
    12721 gtagtgcccg aagtagccag tcgtcaccat gtcgaggtca ttacagcctg tatcgaggag
    12781 gcattggcag aagcagggat taccgaagag gacgtgacag ctgttgcggt tacctacgga
    12841 ccaggcttgg tcggagcctt gctagttggt ttgtcagctg ccaaggcctt tgcttgggct
    12901 cacggacttc cactgattcc tgttaatcac atggctgggc acctcatggc agctcagagt
    12961 gtggagcctt tggagtttcc cttgctagcc cttttagtca gtggtgggca cacagagttg
    13021 gtctatgttt ctgaggctgg cgattacaag attgttgggg agacacgaga cgatgcagtt
    13081 ggggaggctt atgacaaggt cggtcgtgtc atgggcttga cctatcctgc aggtcgtgag
    13141 attgacgagc tggctcatca ggggcaggat atttatgatt tcccccgtgc catgattaag
    13201 gaagataatc tggagttctc cttctcaggt ttgaaatctg cctttatcaa tcttcatcac
    13261 aatgccgagc aaaagggaga aagcctgtct acagaagatt tgtgtgcttc cttccaagca
    13321 gcagttatgg acattctcat ggcaaaaacc aagaaggctt tggagaaata tcctgttaaa
    13381 accctagttg tggcaggtgg tgtggcagcc aataaaggtc tcagagaacg cctagcagcc
    13441 gaaatcacag atgtcaaggt tatcatcccc cctctgcgac tctgcggaga caatgcaggt
    13501 atgattgcct atgccagcgt cagcgagtgg aacaaagaaa actttgcaaa cttggacctc
    13561 aatgccaaac caagtcttgc ctttgatacc atggaataaa gagtggctct ttgtcaagtg
    13621 tagtgggtag gcgaaaagct acaatctgga gagtacgaaa ttcgtactct cttttttgat
    13681 gttcaaagtc atcaaaatcc gttttttaaa attgttaaag ttccggaaac caaaggcttt
    13741 gcacttgata accttaatca aattattggt cgcttcaagt ttagcgttgg aataaggcag
    13801 cttaagtgcg ttgataatct tatctctatc ccttaaaaaa gtccaaaaga ccgtttgaaa
    13861 aagcggatga accgttggca gttcttgctc aatcaatcca aagaaatggt cggcattctt
    13921 ctcttgaaaa tgaaagagca ggagttgata gagttcgtag taaacctgta atccctcaga
    13981 gtaagaaagc agcttatctc gtacttcccg atgggttaag tgcattcgaa acgtgggctg
    14041 atagaatcgt ttctgacaga gcttgaagct atcctggtgt agtaaatcag tccagtggac
    14101 tgatttagcc cgagcctaga aacgaaaaag cgagggttcc aaaagcgctt gagcgcccga
    14161 tactccaagg attttcggtc aaattggttc atgatttgga ttcgtactcg gttcatagct
    14221 cggctcagat gttggacaat gtggaagcgg tcaagaacaa tcttcgcctt tggaaagagt
    14281 tgcttagcga gccgataata agggctgtac atgtccatgg tgatgacctc gaccagctcc
    14341 cgaacctctc tctgatagcg ttggaaatgg tttcgaatca ccgcatgagt tcgcccgtct
    14401 aaaattgcga ggatggattt ggactcaaaa tcttgggcaa tgaagctcat tttgctcttt
    14461 ttgaagctat actcatccca actcataact tttggcaact tggtccaatc ggtttcaaac
    14521 ttaaattccc tcagttttcg gatgatggtg gaagttgaga ccgccaatct gtgggcaatc
    14581 tcagtcatag actgcttttc aaggaggagc tgagcgattt tttgtctcac catgttggaa
    14641 atctggcaat ttttcttgac aatggatgtt tgagaaacga ccaccttaag gcaattcttg
    14701 cactgaaagc ggcgcttttt gagatgcagt accgtgggta aaccctgaca gtcgagaagc
    14761 ggaattttag aggctttctg gaagtcgtat ttgatcatct tcccttgaca atgaggacaa
    14821 ggaggagcgg gggaatccaa ctttgcctga acgactagat gggtttggta tttcagaaca
    14881 gacaagattt tgatattttt gtcttccaat ccgagcaaat ctgtggtatt cttaagttgt
    14941 tccatatgaa ccctttctaa tgagttggtt agacgctttt cattataggt catatggggc
    15001 tttttttcta caagaaacga ccctataatt cctggggtgg gattacccac tacagaaatt
    15061 atagagccaa aacattccaa agtcttgtct gactctggaa tgttttctgt tcacagttgt
    15121 tttgtaactg cctaataagt aaccgcgcta taacgaggta tctaccgaga ttgtcaattc
    15181 tccagtatac tgttataaaa aacaaaactg gaggattttt ttatgtcccc ccatcgtccc
    15241 atcgactgtt ccgtagctgc ccaataacgg agtataatca gatgaaacaa gaataggaca
    15301 aaagtgcctc aagaaaagca tcgtaatttg acaatattat tctgagatgt ttttgatgta
    15361 agcccatatt ttctcaatgg gattatactc gggtgaatag gtaggaagtg gtaacagtct
    15421 atgccctgct ccttacacat gttcattctg tgaaaccttg cattgtccat aatgataagg
    15481 gatggtgtat ctaaagtagg tagtaagaat ttttggaagc aagcttcgaa aaagtcactc
    15541 gtcatcgtat ctttataggt catcggagcc atagtatatt gaaacaaaaa agggcattag
    15601 tacttctata acagtatcgc aatctctggt gtgcgttgtc tcgatcttaa ttcaaataat
    15661 tatatcttct tccagagaga ctaactgcct gctcaaggct cgatcatatt cgagataaaa
    15721 ataagtctca acccctgtct cataaatata aataggagtc aggtagctta agtgattcaa
    15781 ttctttaagg aaccgattta ctttttctgg gtcttgttcg cagtaggtac ggctcttttt
    15841 tagactatat cccatagcct ttagagcgta atgaatagtt gttggaggac agttgaattc
    15901 agcagctatt tcagtcaaat aagcatcggg atgagtttca atatagatct ttaatttatc
    15961 tctatcaact ttttcaatta atagtggaag ttttcttaaa gttagtgtgt tgaaacgttt
    16021 ttatacagtg ctaaaatttg aagtgtagtg cagatgtatt tgtaatagta ttatagcaat
    16081 tctttggaga aggtcgagac aagagaaaat aggtatcttt gagatataag catgatttac
    16141 tataaaatta ttgagttatt cataaaaatt tctctatcaa atatcataat gcgtcgttta
    16201 aggagatggt tacaatgcaa tattaaggag atggttacaa tgcaatatag ttgtggaaaa
    16261 ataaatatta atatccctga tggatatgga gacattaagg atatagtgtt ttcggctcat
    16321 attatagtta ggtataataa tggacattgt ggaggtatag atccgcatat aattggactt
    16381 tgtaaaaagc agatcagaag gatgtctcta tatcctattt tgataatcgt atctagagat
    16441 tcaaag
//
LOCUS       DAVKWZ010000044        16108 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000044 DAVKWZ010000000
VERSION     DAVKWZ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16108
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.044"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            395..778
                     /locus_tag="VW854_001520"
     CDS             395..778
                     /locus_tag="VW854_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEV0262735.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            830..1519
                     /locus_tag="VW854_001521"
     CDS             830..1519
                     /locus_tag="VW854_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0262736.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQLTLIVQNYWQFSSQIGNLF
                     WIQNILSLLFIGVMIVVLVKTGHGYLFRIPRKKWLWYSILTVLVVVFQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYIALFFLVPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFLSLPSLLDIFVFATFGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            1561..1794
                     /locus_tag="VW854_001522"
     CDS             1561..1794
                     /locus_tag="VW854_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262737.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            1945..2556
                     /locus_tag="VW854_001523"
     CDS             1945..2556
                     /locus_tag="VW854_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0262738.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGDFFQNESALIIVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGLAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            2717..3511
                     /locus_tag="VW854_001524"
     CDS             2717..3511
                     /locus_tag="VW854_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEV0262739.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            3508..4143
                     /gene="trmB"
                     /locus_tag="VW854_001525"
     CDS             3508..4143
                     /gene="trmB"
                     /locus_tag="VW854_001525"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEV0262740.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            4269..4748
                     /gene="rimP"
                     /locus_tag="VW854_001526"
     CDS             4269..4748
                     /gene="rimP"
                     /locus_tag="VW854_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEV0262741.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEIISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVRIPYSLVSKARLAVKL"
     gene            4792..5928
                     /gene="nusA"
                     /locus_tag="VW854_001527"
     CDS             4792..5928
                     /gene="nusA"
                     /locus_tag="VW854_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEV0262742.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            5950..6243
                     /locus_tag="VW854_001528"
     CDS             5950..6243
                     /locus_tag="VW854_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEV0262743.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            6236..6535
                     /locus_tag="VW854_001529"
     CDS             6236..6535
                     /locus_tag="VW854_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEV0262744.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLAKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            6552..9344
                     /gene="infB"
                     /locus_tag="VW854_001530"
     CDS             6552..9344
                     /gene="infB"
                     /locus_tag="VW854_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEV0262745.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKTEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     AKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            9595..9945
                     /gene="rbfA"
                     /locus_tag="VW854_001531"
     CDS             9595..9945
                     /gene="rbfA"
                     /locus_tag="VW854_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEV0262746.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
     gene            10224..11003
                     /locus_tag="VW854_001532"
     CDS             10224..11003
                     /locus_tag="VW854_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262747.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELGPQDIPTL
                     LEQGPTSLLSQEILDRVANACIRDNVLLASGTSVLAGLPGGLAMAITIPADVAQFYGF
                     SLKLAQELGYIYGYEDLWASRDELSEDAQNTLLLYLGVMLGVNGTAALLRAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLRIFGVNLTKGGLAKGMGKFIPILGGIISGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIEGE"
     gene            11005..11187
                     /locus_tag="VW854_001533"
     CDS             11005..11187
                     /locus_tag="VW854_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262748.1"
                     /translation="MGLIKTLAKIYGNYFLTVQGVKVMKTIKKDDNAVVGLGKLFIAD
                     KLMDTARWLIKPEERE"
     gene            11184..11324
                     /locus_tag="VW854_001534"
     CDS             11184..11324
                     /locus_tag="VW854_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262749.1"
                     /translation="MKFFWGLLAIIFIKPIIGIVKFFWMIISFAVQLLFYKILDWFFK
                     LI"
     gene            complement(11532..11606)
                     /locus_tag="VW854_001535"
                     /pseudo
     CDS             complement(11532..11606)
                     /locus_tag="VW854_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            11637..11870
                     /locus_tag="VW854_001536"
     CDS             11637..11870
                     /locus_tag="VW854_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEV0262750.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            11870..13204
                     /locus_tag="VW854_001537"
     CDS             11870..13204
                     /locus_tag="VW854_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEV0262751.1"
                     /translation="MTDERIHILRDILLELHNGASPESVQDRFDATFTGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVLDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYELMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWIQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKAIMKGLREGTKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            13214..13468
                     /locus_tag="VW854_001538"
     CDS             13214..13468
                     /locus_tag="VW854_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEV0262752.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKAGKGFHDLPEGLFGERNY"
     gene            13564..13959
                     /locus_tag="VW854_001539"
     CDS             13564..13959
                     /locus_tag="VW854_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEV0262753.1"
                     /translation="MSKKLNRKKQLRNGLRRSGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLDLKGIGPATIKKLKENGIKFK"
     gene            14371..14820
                     /locus_tag="VW854_001540"
     CDS             14371..14820
                     /locus_tag="VW854_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEV0262754.1"
                     /translation="MIGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEK
                     LERLGVHYEISERTQIPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVAPELLDE
                     KGHFKNQLFAPTYFMGDGYQRVYRYLDKRVDMKGGFIKKARKKDGKN"
     gene            14807..15364
                     /locus_tag="VW854_001541"
     CDS             14807..15364
                     /locus_tag="VW854_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0262755.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIFDYASLPEVAYPAGFP
                     PVKTLEDEIYYLEHILPERNQKENLPAGYGIVVKVTDKIVGSVDFNHRHEDDVLEIGY
                     TLHPDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSKRVAEKLGFTLEARI
                     RDRKDVQGNRCDSLIYGLLKSEWEE"
     gene            15366..>16108
                     /locus_tag="VW854_001542"
     CDS             15366..>16108
                     /locus_tag="VW854_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HEV0262756.1"
                     /translation="MSDVKEEVSSLSEKQLRQIDVEYAELNDSDIIERLAYLEINNNE
                     KRIVISDIEPTKEIMSVSDQIFEIQKNFQKIKNMFELFISDVSDFLSIKNKLESKELE
                     IEEADVNRFMIHLLSSGKLFVDFNENQIKQKYSKDSEEFDCIHGFASYQYDINFTYRF
                     CHSLRNYSQHTDLPINEVKAVSPDDETVIIDFYIDLDYLLNSNFKWKKLKGELIKLNQ
                     ETSKIDAIALVKEYFNALTELYGNYNKLF"
BASE COUNT         4910 a         2896 c         3660 g         4642 t
ORIGIN      
        1 gtagataatt tttttagaga cttaaccccg ttactgttca ttttaaatat tattggcctg
       61 tcttgctttt cagtgctaac atacattaac ataaaagaga tactcttaaa tataagtaaa
      121 tgatgatttg gttagtccta gaaccaatca atttatgttt atcggcttgg cagattttat
      181 gtttgtcatt tgtttgtata gaggtatttc agaaacagaa ttctatcaac aactaatcgc
      241 ttatataggt gtattctctg cttgtttatc acgcttctgc tcatgtgggg cttgaagtat
      301 tataaaaagt agctttctat ccttttctga caggatattt gagtagaagg ctctattaaa
      361 ttgttagtga aataaatgaa tggaggtatt taatatgaaa tacagattat tttttgttat
      421 tttcttgagt agtgtgttgg atattctttt agggacattt ttacaaatct ctatcgtatc
      481 tattgggtgg cttgttcttt acagtggttt gtttgaagca ggagttttcc ttcttgctaa
      541 taaaggggtg gcggtaaaaa tcaaggaagt agatattcga aatcgcttta aatttatttt
      601 tggaaaaacc ttatggtttc aaattctttt gctcatcttt ttgataatca aactttatct
      661 tggtttggat gcgaggttga ttttattcta tggacatatt ttcattgtct ttaatgcctt
      721 aatgtatcta ttatctagta gtcaggttag ccttaaaaaa aacaaactgt cttcttaatc
      781 ttacagcagt agagcctgac aaggtgaggt catagtaggg aggatttcta tgaaaaagta
      841 tcaacttcta ttcaaaataa gtgcagtctt ctcttactta tttttcgtat ttggtctttc
      901 tcagctgacg cttatcgtcc aaaactattg gcaattttct tctcagatag gcaatttatt
      961 ctggattcaa aatatcttga gtttactttt tattggagtc atgattgtgg ttcttgttaa
     1021 gacaggccat ggttatctct tccgcattcc aagaaaaaaa tggctttggt attcgatttt
     1081 gacagtatta gtggtagtgt tccagatctc ttttaacgtt cagacagcta aacatgttca
     1141 gtcaactgcg gaaggttggg ctgtattgat tggttatagt gggactaact ttgcagagct
     1201 aggtatttat atagccctgt tctttctggt tccactgatg gaagaattga tttatagagg
     1261 attactgcaa catgctttct ttaagcattc gcgatttggc cttgatttgc ttcttccttc
     1321 cattttgttt gctcttcctc attttttgag tctgcctagt ctgttagata tcttcgtctt
     1381 tgcaacattt ggaatcatct ttgccggttt gacccgctat accaagagta tttatccatc
     1441 ctatgcggtg catgtgatca ataatattgt agcgaccttc ccgtttttgc tcacttttct
     1501 acatagggtc ttggggtaaa aaaacaaaag gcttgctttt cagccataga ggaggtcatc
     1561 atgtataaac acttattttt cctagattcc aaaactttag atcggttgac accctatatt
     1621 ctagtcttgg cttctgacac cattgctttt aatgtttttg tgctaacctt tgtatctgcg
     1681 gtggttttta atttcctaaa ttccatgcta gctttaatgg ctatattcat aggggctggc
     1741 tatgtggtcg gattttggtt actaatactc aatgaaaatc aaagagcaaa ctaggaaact
     1801 agccgcaggc tgtacttgag tacggcaagg cgacgttgac gtggtttgaa tttgattttc
     1861 gaagagtata aaatggtttg ttttggaaag gctagaacta aagaatgacg tgtaggaagt
     1921 ctgtttgttg aggaggataa ttttatggag ttttttgata aatttcatgc cttgtgtttt
     1981 ggatttttag tactaataat tgtcattaca gttccttata cgattaacca tggggatttt
     2041 tttcaaaatg aatctgcatt gattattgta agtcttcttg taacctcgct gagtgttgct
     2101 tatgctagaa agtttgaaat gatttctttt gggatgttaa gcaagaaaca acttttgctt
     2161 ttcattgcaa tctttcttct aagtgtactt gagacgctgg tttatattca tttcttcgct
     2221 gtttcttctg gctcaggggt ccaacacttg gcggaagtca gcagaggaat ttccctgtct
     2281 ttgattttga ctacctcagt ttttggcccc atccaggagg aactcatttt cagaggactt
     2341 cttcaaggtg cggtttttga caattcttgg ttagggcttg tgctaacttc ctctctcttt
     2401 tctttcatgc atggaccttc taatgtccct tcgtttattt tttatctact tgggggcttg
     2461 ttgctgggcc ttgcttataa aaagagccaa aacctatggg tttctactct agtccacatg
     2521 ttttacaaca gttggccact cctatattat ttataaaaat tatgaaaagg taagcagaag
     2581 acggtgctta cctttttcaa gctataatga tacccaagcc aatccagagg cttttctttg
     2641 agaatcgggt tagagtggtt gacgaatagg ccaaaaacta gtagaatagt aaggaaactt
     2701 tatacggagg aaagaaatgg atttgggtga taatgagcta acactgactc ccatacctgg
     2761 gaaaagtggc aaggcttata tgggtagcta tcctgatggg aagcgcatct ttgtaaaaat
     2821 gaacacctct ccaatcctac ctggtctagc tagagaacaa attgctccac aattattatg
     2881 gagtcgtcgt ttggcagatg ggcgtgatat gtgtgctcaa gaatggttga caggcaagat
     2941 attgaccccc tatgatatga atcgtaagca aatcgtcaat attttaaccc gtcttcatcg
     3001 ctcacgtccg ttgatgacac aattgagtcg tttgggctat gccatggaaa cacctgtaga
     3061 tttactacag tcttggcaag aaacggctcc agatgctttg cgtaaaaatc attttatcag
     3121 tgaagtgatg gctgatttgc gtcagactat tccaggattt agagaggacc atgcgaccat
     3181 tgtccatgga gatgtacgac atagtaattg gattgagaca gatagtggct tgatttattt
     3241 agtagattgg gattcggttc gcttgaccga tcgcatgttt gatgtggccc atatgctctg
     3301 ccattatatt tcagaacatc agtggaagga atggttgacc tactacggtt acaagtacaa
     3361 tcaaacggta ttaagtaaat tgtattggta tggtcaattg tcttatttga gtcagatttc
     3421 caagtattat atgaaccaag atttagaaaa tgtcaatcgg gagattcatg gtttgcgtca
     3481 tttccgagac aagtatggaa agagaagatg agagttagaa atcgtaaagg ggcaacagaa
     3541 ttactagagg caaatcccca gtatgtggtc ctcaatccct tggaagccaa ggcaaaatgg
     3601 cgggacttgt ttggcaatga taatcccatt catgtggaag ttggaagtgg aaagggtgcc
     3661 tttgtttcag gtatggccaa gcaaaaccct gacatcaact atatcgggat tgatattcaa
     3721 aaatctgttt tgagctacgc tttggacaag gtgcttgaag ttggagtgcc taacatcaag
     3781 ctcttgtggg tagatggttc tgacttgact gactactttg aagacggtga gattgatcgc
     3841 ttgtatctga acttttcaga tccatggccg aaaaaacgcc atgaaaagcg tcgtttgacc
     3901 tacaagacct ttttggacac cttcaaacgt atcttgcctg aaaatggaga aattcatttc
     3961 aagacggata accgtggctt gtttgagtac agtttagtga gtttttctca atatggcatg
     4021 aaactcaatg gtgtctggct agatttgcat gccagtgatt ttgaaggcaa tgtcatgaca
     4081 gaatacgagc aaaaattctc aaacaagggg caagttatct accgagttga ggcagaattt
     4141 taagagatag cctaaaatta ggctgtacaa gtgcttttgc tttacataag ttggcaaacg
     4201 tgctatactg atagtaagaa tatgaaaagt gaggcgggga aatatcttcg cctcttgctt
     4261 atgaggaggt ggacgcaatc gcaacaatcg tagaattagt cagagaagtt gtagaacctg
     4321 tcatagaagc tccttttgaa ctcgtggata tcgagtatgg aaagattggc agtgacatga
     4381 ttctcagtat ttttgtagat aaacccgaag gaattacctt gaacgacacg gcagacttga
     4441 cagaaattat cagtcctgtc ctagacacca tcaagccaga tcccttccca gaacaatatt
     4501 tcctagaaat taccagtcca ggcttggaac gtcctttgaa aaccaaggat gccgtcgctg
     4561 gagcggttgg aaaatacatc catgtcggac tctaccaagc catcgataag caaaaggtct
     4621 ttgaaggaac tttgttggcc ttcgaagagg acgagttgac tatggaatat atggacaaga
     4681 cgcgtaagaa aaccgtccga attccataca gtttagtatc aaaagcacgt ttagcagtta
     4741 aattatagaa aaagaaagga tagcttttga ggattcaaaa gtgaagaaaa catgagtaaa
     4801 gaaatgctag aggccttccg cattttggaa gaagacaagg gaatcaaaaa agaagatatc
     4861 atcgacgcag tagtagagtc gcttcgttcc gcttatcgca gacgctatgg tcagtcagac
     4921 agcgtagcta ttgacttcaa cgaaaaaaca ggtgacttta cagtttatac tgtccgtgaa
     4981 gttgttgatg aagtatttga tagccgtttg gaaatcagct tgaaagatgc tcttgccatt
     5041 aattcagctt atgaacttgg agacaaaatc aagtttgaag aagcaccagc tgagtttggt
     5101 cgtgtagcag cccaatctgc caaacaaacc atcatggaaa aaatgcgcaa gcaaacacgt
     5161 gccatcactt acaatactta caaagaacat gagcaagaaa tcatgtctgg tacagtagaa
     5221 cgctttgaca accgctttat ctatgtcaac cttggtagca tcgaagccca attgtcaaaa
     5281 caagaccaaa ttcctggaga agtttttgct tctcatgatc gtatcgaagt ttatgtttac
     5341 aaggttgaag acaaccctcg tggtgtgaac gtctttgtta gccgtagcca tccagaaatg
     5401 atcaaacgtt tgatggagca agaaattcca gaagtttatg atggaactgt tgaaatcatg
     5461 agcgtggctc gtgaagcagg tgaccgtacg aaggttgctg ttcgtagcca caatccaaac
     5521 gtggatgcta tcggtacaat cgttggacgt ggtggtgcta atatcaagaa gattactagc
     5581 aaattccacc cagctcgtta cgatgctaaa aatgaccgca tggtaccaat cgaagaaaat
     5641 atcgatgtta tcgagtgggt agcagatcca gctgaattta tctacaatgc catcgctcct
     5701 gctgaggttg accaagttat ctttgatgaa aacgacagca aacgtgcctt ggtggttgtt
     5761 ccagataaca agctttctct tgccattggt cgtcgtggac aaaacgtgcg cttggcggct
     5821 cacttgactg gttaccgtat cgatatcaag tctgctagcg aatttgaagc catggaagac
     5881 gctgcttcag tagagttgga agtagaaaac gatactgtag aagaataaaa gctgctagag
     5941 gagggaaaga tgaaaacaag aaaaatccct ttgcgcaagt ctgttgtgtc taacgaagtg
     6001 attgataagc gtgatttgct ccgcattgtc aagaacaagg aaggacaagt ctttattgat
     6061 cctacgggca aggccaatgg ccgcggcgct tatatcaaac tagacaatgc agaagcccta
     6121 gaggcgaaaa agaagaaggt ctttaaccgc agctttagca tggaagtgga agaaagcttt
     6181 tatgacgagt tgatcgctta tgtggatcac aaagtgaaaa gaagagagtt gggacttgaa
     6241 taagcaaaag ataagtaatc tcttggggct tgctcagcga gcagggcgca tcatatcggg
     6301 tgaagaattg gtggtcaagg ccattcaaga cggcaaggcc aagttggtct ttctagctca
     6361 tgatgctgga cccaatctgg ccaagaagat tcaagataaa agtcattatt atcaagtaga
     6421 aattgtaacc gtgttttcaa cactggaatt aagcatagca gtcgggaaat cgagaaaggt
     6481 tttggctgta acagatgctg gatttacaaa gaaaatgagg tctcttatgg aatagaagag
     6541 gaggacatga tttgtctaag aaaagattgt acgaaatcgc aaaagaactt ggaaaagaaa
     6601 gtaaagaagt tgtagcgcgt gcaaaagagt tgggcttgga tgtgaaaagc cactcatcaa
     6661 gtgtggaaga agctgtcgct gcaaaaattg ctgccagctt taagcctgca gctgctccga
     6721 aagtagaagc aaaacctgca gcaccaaaag taagtgcaga aaagaaaacc gaaaaatctg
     6781 agccagctaa accagctgta gctaaggaag aggcaaaacc ggctgagcca gttgctccga
     6841 aaacagaaaa agtagcagcg aaaccgcaaa gccgtaattt caaggctgag cgtgaagccc
     6901 gtgctaaaga gcaggcagaa cgacgcaagc aaaataaggg caataaccgt gaccaacaac
     6961 aaaacggaaa ccgtcagaaa aacgatggcc gtaatggtgg aaaacaaggt caaagcaacc
     7021 gcgacaatcg tcgctttaat gaccaagcta agaagcagca aggtcagcaa aaacgtagaa
     7081 atgagcgccg tcagcaagag gacaaacgtt caaatcaagc ggctccacgt attgacttta
     7141 aagcccgtgc agcagcccta aaagcagagc aaaatgcaga gtacgctcgt tcaagtgagg
     7201 aacgcttcaa gcagtatcag gctgctaaag aagccttggc tcaagctaac aaacgcaagg
     7261 aaccagagga aatctttgaa gaagcggcta agttagctga acaagcacag caagttcaag
     7321 cagtggttga agtcgtccct gagaaaaaag aacctgcagt ggatacacgt cgtaaaaaac
     7381 aagctcgacc agacaaaaat cgtgacgatt atgatcatga agaagatggt cctagaaaac
     7441 aacaaaagaa tcgaagtagt caaaatcaag tgagaaatca aaagaatagt aactggaata
     7501 acaacaaaaa gaacaaaaaa ggcaataaca agaacaaccg taatcagact ccaaaacctg
     7561 ttacggagcg taaattccat gaattgccaa cagaatttga atatacagat ggtatgaccg
     7621 ttgcggaaat cgcaaaacgt atcaaacgtg aaccagctga aatcgttaag aaacttttca
     7681 tgatgggtgt catggccaca caaaaccaat ccttagatgg ggaaacaatt gaactcctca
     7741 tggtggatta cggtatcgaa gccaaacaaa aggttgaagt ggataatgct gacatcgaac
     7801 gtttctttgt cgaagatggt tatctcaatg aagatgaatt ggttgagcgt ccaccagttg
     7861 ttactatcat gggacatgtt gaccacggta aaacaaccct tttggatact cttcgtaact
     7921 cacgtgttgc gacaggtgaa gcaggtggta ttactcagca tatcggtgcc taccaaatcg
     7981 tggaaaatgg taagaagatt accttccttg atacaccagg acacgcggcc tttacatcaa
     8041 tgcgtgcgcg tggtgcttct gttaccgata ttacgatctt ggtcgtagcg gcagatgacg
     8101 gggttatgcc tcagactatt gaagccatca accactcaaa agcagctaac gttccaatca
     8161 tcgtagccat taacaagatt gataaaccag gtgctaaccc agaacgcgtt atcggtgaat
     8221 tggcagagca tggtgtgatg tcaaccgctt ggggtggaga ttctgaattt gttgaaatct
     8281 cagctaaatt caaccaaaat atcgaagaat tgttggaaac agtccttctt gtagctgaaa
     8341 tccaagaact caaggcagac ccaacagtgc gtgcaatcgg tacggttatc gaagcgcgct
     8401 tggataaagg aaaaggtgcg gtcgcaaccc ttcttgtaca acaaggtacc ttgaatgttc
     8461 aagacccaat cgttgtcgga aataccttcg gtcgtgtccg tgctatgacc aacgaccttg
     8521 gtcgtcgtgt taaagttgct ggaccatcaa caccagtctc tatcacaggt ttgaacgaag
     8581 caccgatggc gggtgaccac tttgccgttt acgaggatga aaaatctgcg cgtgcagcag
     8641 gtgaagagcg tgccaaacgt gccctcatga aacaacgtca agctacccaa cgtgttagcc
     8701 ttgaaaacct ctttgatacc cttaaagctg gggaactcaa atctgttaat gttatcatca
     8761 aggctgatgt acaaggttct gttgaagccc tttctgcctc acttcaaaag attgacgtgg
     8821 aaggtgtcaa agtgactatc gtccactcag cggtcggtgc tatcaacgaa tcagacgtga
     8881 cccttgccga agcttcaaat gcctttatcg ttggtttcaa cgtacgccct acaccacaag
     8941 ctcgtcaaca agcagaagct gacgatgtgg aaatccgtct tcacagcatt atctacaagg
     9001 ttatcgaaga gatggaagaa gctatgaaag ggatgcttga tccagaattt gaagaaaaag
     9061 ttattggtga agcggttatc cgtgaaacct tcaaggtgtc taaagtggga actatcggtg
     9121 gatttatggt tatcaacggt aaggttgccc gtgactctaa agtccgtgtt atccgtgatg
     9181 gtgtcgttat ctatgatggt gaactcgcaa gcttgaaaca ctataaagac gacgttaagg
     9241 aagttacaaa cggccgtgaa ggtggattga tgatcgacgg ctacaatgat attaagatgg
     9301 atgatgtgat tgaggcgtat gtcatggaag aaatcaagag ataagatttt ttgctccttt
     9361 cttaggtggt gagggacgca agcaaaccga tggtttcatt gcttattttt gagcctaggg
     9421 tctcaaaaat cccctgtgat gggactgata aatcagttcc atcactttca ccacggcgaa
     9481 agaagcagat gacttcaaat tgaacttcgt ttcaatttaa actgaaaatc aagaagttta
     9541 aaatagctag gtctgctggc ctagcttttg gttcaaagta gagaaaggaa tatcatggca
     9601 aatcatttcc gtacagatcg tgtgggcatg gaaatcaagc gtgaagtcaa tgagattttg
     9661 caaaagaaag tccgtgatcc acgtgtccaa ggtgtgacca tcacagatgt tcagatgttg
     9721 ggtgacttgt ctgttgccaa ggtttattac accattttga gtaaccttgc ttcggataac
     9781 caaaaagccc aaatcgggct tgaaaaagca actggtacca tcaaacgtga acttggtcgc
     9841 aatttgaaat tgtacaaaat cccagatttg accttcgtca aagacgagtc catcgagtat
     9901 ggaaacaaga ttgacgagat gctacgcaat ctggataaga actaaagaag aggggttgcc
     9961 cctctttttt ggtggaggaa aataggttga atttgaaatg gaaaaatatt cttttaaaat
    10021 atattgaaac tagaatagta cgcctctact tctaaaacat tgttagaaat cgatttgact
    10081 gtcctgatcg atttgtcctg ttcttgtttc attttactat aaaaaagggg ttctgtattt
    10141 tttaatgtta tctaattaga aaatgctttt tttgtaggaa atataatatg ataaggtgta
    10201 aaaaagaaat aaggagtttg tatatggctg aacaagactt agctatgcaa gtattacaac
    10261 aagtggtaaa actaccagtt gttaaggttg atcgttcgaa atttttagtg gataagtttt
    10321 ccaaagaatt gggtccacag gacattccta ccttattgga acaaggtcca acgtctctct
    10381 tatctcaaga aatcttagat cgagtggcta atgcctgtat tagggataat gttttattag
    10441 cgagtgggac ttctgttttg gcaggattac ctggagggct tgctatggca attaccattc
    10501 cagctgatgt ggctcaattt tatggtttct ctctgaaatt ggcccaagaa ttaggttata
    10561 tttatggtta tgaggatctt tgggcttcac gcgatgagtt gagtgaagat gctcaaaata
    10621 cccttttgct ttatctaggc gtcatgttgg gggtgaatgg aactgctgct ttgctacgtg
    10681 ctggtggtat aacaattgcc aaacaggtaa tgaaaacagt acctaataaa gctttaacaa
    10741 agacgctttg gtatcctatt ttgaaaaaag tcttaagaat atttggtgtg aatcttacca
    10801 agggagggct ggccaaagga atggggaaat ttattcctat cttgggtggt atcatttcag
    10861 gtggtttaac ctttgcaact atgaaaccga tgggtgaaag cttgcagaaa gaattgtcca
    10921 agctagttaa ctatagtgaa gttcaatatc aagaagatgt tgaaacaatc cgaaaagagg
    10981 ctgaaatcat cgaaggagag taatatggga ctcataaaaa ctctagctaa aatttatggt
    11041 aattactttt tgaccgtgca aggtgtaaaa gtaatgaaaa cgataaagaa agatgataat
    11101 gccgttgtcg gtctggggaa actttttatt gccgacaagt taatggatac ggctcggtgg
    11161 ctcattaagc cagaggagag agaatgaaat ttttctgggg acttcttgcc attattttta
    11221 tcaaaccgat tattgggatt gtgaaattct tttggatgat catttctttt gcagttcaat
    11281 tgctgtttta caagatattg gattggttct ttaaacttat ctagatggta atccaagtcg
    11341 cagaaaacta gcaggaactc cactgctagt tttttaatct ctctccatat ggtataatat
    11401 aagcagtaaa atcattttat actcttcgaa aatctcttca aaccacgtca gcttcacctt
    11461 gcagtatata tgttactgac ttcgtcagtt ctatccacaa cctcaaaacg gtgttttgag
    11521 ctgacttcgt cagttctatc tacaacctca aaacactgtt ttgagcaacc tgcggctagc
    11581 ttcctagttt gctctttgat tttcattgag tattagaaga tacaatggag gtcgtcatgg
    11641 acaatatcat cgatgtgtca attcctgttg cagaagtggt ggacaagcat ccagaagtct
    11701 tggaaattct agtggagttg ggttttaaac cccttgccaa tcccttaatg cgcaatacag
    11761 ttggtcgtaa agtatcactt aaacagggtt ctaagctagc aggaactcct atggacaaga
    11821 ttgtacgcac actggaagcg aatggctacg aagtgattgg attagactaa tgacagatga
    11881 acggattcat atcctacggg atattttgtt agaattgcac aatggcgcct ctcctgagtc
    11941 ggttcaagat cgctttgatg cgacctttac gggcgtgtca gccatcgaga tttcccttat
    12001 ggagcacgag ctgatgaact cggattcggg cgtcactttt gaagatgtta tggaactctg
    12061 tgatgtccat gccaatcttt ttaaaaatgc tatcaaaggt gtcgaagttt tagataccga
    12121 acaccctggg cacccagttc gtgtctttaa ggaagaaaat ttggccctcc gtgcagcttt
    12181 gattcgcatt cgtagattgt tagataccta tgagttgatg gaagacgagg aaatgctggc
    12241 ggagatgcgc aagggcttgg tgcgtcagat gggacttgtg gggcaatttg acatccatta
    12301 ccaacgtaaa gaagaactct tctttcctat catggagcgc tatggacacg attcacctcc
    12361 caaagttatg tggggagtgg atgatcagat tagggaactc tttcaaacag ctctaacgac
    12421 agccaagtca ctaccagaag tgtcaattag cagtgtaaag gaagcttttg aagcttttgc
    12481 gacagagttt gaaagtatga ttttcaagga agagtccatc ctcctcatga ttctccttga
    12541 gtcttttact caggatgact ggattcagat tgcggaggag agcgatgcct atggctatgc
    12601 catcatccgt ccgtcagaga aatgggtgcc agaacgacag agctttattg aggaaaagat
    12661 tgcagaggag cctgtacagc tagatacggc agaaggtcaa gttcaacaag tcatagatac
    12721 gccagaaggc cattttacca ttacctttac ccctaaggaa aaggaagctg tgctggaccg
    12781 ccatagtcaa caggcttttg gtaatggcta tctttcagtc gagcaggcca atctcatcct
    12841 caatcatctc cctatggaga ttacctttgt caataaagaa gatattttcc agtattacaa
    12901 tgacaatacg ccagctgatg agatgatttt caaacgaacg ccgtcccaag tcgggcgcaa
    12961 tgtcgaactc tgtcatccgc ctaagtattt ggacaaggtc aaggctatca tgaagggact
    13021 tcgtgagggg accaaggaca agtatgaaat gtggttcaag tctgagtcgc gaggcaagtt
    13081 tgtccacatc acctatgctg cagtacacga tgaagacgga gaattccaag gagtgttgga
    13141 gtatgttcag gatatccagc cctaccgtga gattgatacg gactatttcc gtggattaga
    13201 ataaggagaa aaaatgagtt acgaacaaga atttatgaag gaatttgaag cttgggtcaa
    13261 tacccaaatc atgattaacg atatggcgca caaggaaagt caaaaagttt acgaagaaga
    13321 ccaggacgag cgtgccaaag atgccatgat tcgctacgag agtcgcttgg atgcttatca
    13381 gttcttgctt ggtaagtttg aaaacttcaa agcaggcaag ggattccatg atttgccaga
    13441 aggcttgttt ggtgagcgaa attattaaac gagaaagatt cttgattttt cactaaaatc
    13501 ttgatagaat gtttatgtta aatccttgtc agagcaggga ttttttattg aaaggatttt
    13561 atcatgtcaa agaaactcaa tcgtaaaaaa caattacgaa atggcctccg tcgctcaggt
    13621 gccttttcaa gtactgtgac taaggttgta gatgagacaa aaaaagtcgt gaagcgtgca
    13681 gaacagtcag caagcgcagc tggtaaggct gtttctaaaa aagttgaaca agcagtagaa
    13741 gctaccaaag agcaagctca aaaagtagct aattctgtag aagattttgc agcaaacttg
    13801 ggtggacttc cacttgatcg tgccaagact ttctatgatg aaggaatcaa gtctgcttca
    13861 gatttcaaaa actggactga aaaagaactc cttgacttga aaggaatcgg cccagctacc
    13921 atcaagaaat tgaaagaaaa tggcatcaag ttcaagtaat ttttcttgag ccttgcattt
    13981 ccgagaaaaa cttgctacaa tagagccatt agaggtgttt tgaatcccac attttacaga
    14041 aagtggcggc gctgagaagt ccacaaatgt gtcaaaactg gttgctaatg actgaaaaaa
    14101 tgaaatattt ctgtcttttt attttaaaga ctaaagatgc gggcttggcc cgaaattggg
    14161 tggtaccgcg gataaacaca ttcgtccctg tcatgtagat ggcagggctt ttcttttgtg
    14221 tcttagtcaa aggaggttgt tataaaacaa tcatttaaaa ctaataaact atattatggt
    14281 tttcctgttt ttattttagg atatcaagat tagaattttg gctataacat cacgacctgt
    14341 agttcctctt atagtctagg agattgggtt gtgattggag tcgttgcgag agagaatgcc
    14401 gcagagcaga tcaaacagta tcaaaaattt actgtgaata tttctgatga aacttctatg
    14461 cttgcgatgg agcaggctgg ttttatcagt catcaggaga aattggaacg tttgggagtg
    14521 cattatgaaa tttctgaacg aactcagatt cctattttag acgcctgtcc acttgtttta
    14581 gattgtcggg tagataggat tgttgaggaa gacggtattt gccacatctt tgccaagatt
    14641 cttgagcgac ttgttgcccc agaactcctg gatgaaaagg gacattttaa aaatcaactg
    14701 tttgccccaa cctatttcat gggagatgga tatcagcgcg tttatcgcta tctggataag
    14761 cgtgtagata tgaagggcgg tttcatcaaa aaagcgagga agaaggatgg caagaactga
    14821 gctgccagat aaaatcgaaa cagaacgtct cgttttacga gtccgtactg tggcggatgc
    14881 tgaggatatc tttgactatg ctagtttgcc agaggtcgcc tatccagcag gttttcctcc
    14941 agtcaagacc ttggaagatg agatttatta tctggagcac attcttccgg agcgtaatca
    15001 aaaggaaaat ctcccagcag gctacgggat tgtcgtcaaa gtaacggata agatcgttgg
    15061 ctctgtcgat ttcaaccatc gccatgaaga tgatgtgctg gaaatcggct ataccttaca
    15121 cccagactat tgggggcgag gttatgtgcc agaagctgcg cgtgccttga ttgacttagc
    15181 ctttaaagat ttgggtcttc acaagattga actaacttgc tttggatata accttcaaag
    15241 taaacgagtc gcggaaaagc ttggctttac cctcgaagct cgcataagag accgaaagga
    15301 tgttcaagga aaccgctgtg acagtctgat atatggcttg ctgaagagcg agtgggagga
    15361 ataagatgag cgatgtaaaa gaggaagtaa gctctctatc tgagaagcag ttacgccaaa
    15421 ttgatgttga gtatgctgaa ttaaatgata gtgatattat cgagcgatta gcttatttag
    15481 aaattaacaa caatgaaaaa cgtattgtca tctctgatat tgaaccaacc aaggaaataa
    15541 tgtcagtctc ggaccaaatt tttgagattc aaaagaattt tcaaaaaatc aagaatatgt
    15601 tcgaattgtt tatttcagat gtctcggatt ttttaagtat taaaaataaa cttgagtcaa
    15661 aggaattaga aattgaagag gcagatgtaa atcgtttcat gattcattta ttatccagtg
    15721 gaaaattgtt tgtcgatttt aacgaaaacc aaattaaaca aaaatattcc aaagatagtg
    15781 aggagtttga ttgtatacat ggttttgcaa gctatcagta tgatatcaat tttacttatc
    15841 gattttgcca ttctttgaga aattattcgc aacatactga cttaccaata aatgaggtaa
    15901 aagctgttag cccagatgat gagactgtta taatagattt ctatattgat ttagattatc
    15961 ttttaaattc caactttaaa tggaaaaaat tgaaagggga gttgataaaa ctaaaccaag
    16021 aaacatctaa gattgacgct atagctctag tgaaagaata ctttaatgct cttacagagc
    16081 tctatggtaa ttataataag ttgttttt
//
LOCUS       DAVKWZ010000045        15984 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000045 DAVKWZ010000000
VERSION     DAVKWZ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15984
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.045"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            37..504
                     /locus_tag="VW854_001543"
     CDS             37..504
                     /locus_tag="VW854_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV0262757.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENDEAFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFSGTLQS
                     SEEGEVSWVQKDQIPNLNLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
     gene            complement(501..1016)
                     /locus_tag="VW854_001544"
     CDS             complement(501..1016)
                     /locus_tag="VW854_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEV0262758.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(1028..1555)
                     /locus_tag="VW854_001545"
     CDS             complement(1028..1555)
                     /locus_tag="VW854_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV0262759.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            complement(1833..2396)
                     /locus_tag="VW854_001546"
     CDS             complement(1833..2396)
                     /locus_tag="VW854_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV0262760.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            complement(2380..2931)
                     /gene="coaC"
                     /locus_tag="VW854_001547"
     CDS             complement(2380..2931)
                     /gene="coaC"
                     /locus_tag="VW854_001547"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEV0262761.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            complement(2943..3632)
                     /gene="coaB"
                     /locus_tag="VW854_001548"
     CDS             complement(2943..3632)
                     /gene="coaB"
                     /locus_tag="VW854_001548"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEV0262762.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISADQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            3862..5532
                     /locus_tag="VW854_001549"
     CDS             3862..5532
                     /locus_tag="VW854_001549"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEV0262763.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELEIDQRR
                     ILWERVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNALSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            5860..7035
                     /gene="mutY"
                     /locus_tag="VW854_001550"
     CDS             5860..7035
                     /gene="mutY"
                     /locus_tag="VW854_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEV0262764.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            7090..7794
                     /locus_tag="VW854_001551"
     CDS             7090..7794
                     /locus_tag="VW854_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0262765.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQSIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            7787..9136
                     /gene="vicK"
                     /locus_tag="VW854_001552"
     CDS             7787..9136
                     /gene="vicK"
                     /locus_tag="VW854_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEV0262766.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            9138..9947
                     /locus_tag="VW854_001553"
     CDS             9138..9947
                     /locus_tag="VW854_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV0262767.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            10038..10280
                     /locus_tag="VW854_001554"
     CDS             10038..10280
                     /locus_tag="VW854_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEV0262768.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            10282..10536
                     /locus_tag="VW854_001555"
     CDS             10282..10536
                     /locus_tag="VW854_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEV0262769.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEGPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            10583..11278
                     /locus_tag="VW854_001556"
     CDS             10583..11278
                     /locus_tag="VW854_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEV0262770.1"
                     /translation="MKKKWLFADYYDTTIILLALISVILVLLGFAEMTDLDNPPYSII
                     DLVIWGVFVIDYSWRFFITKRKWRFILENIFDLLTILPLNAIFTVFRLGRIFRLARLT
                     KLLKLTRLLRIIGLTGKLERKISRFLRTNGLIYILYVNIFIVLVGSSILSVVEEKSFS
                     DSLWWALVTVTTVGYGDIVPVSLFGKWLAVLLMLVGIGTIGMLTSALTNFFVKDNPDE
                     QIKLDKLKDELSS"
     gene            complement(11627..12613)
                     /locus_tag="VW854_001557"
     CDS             complement(11627..12613)
                     /locus_tag="VW854_001557"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEV0262771.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            12807..15275
                     /gene="gyrA"
                     /locus_tag="VW854_001558"
     CDS             12807..15275
                     /gene="gyrA"
                     /locus_tag="VW854_001558"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEV0262772.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMVGQILSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLTEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            15275..>15984
                     /gene="srtA"
                     /locus_tag="VW854_001559"
     CDS             15275..>15984
                     /gene="srtA"
                     /locus_tag="VW854_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEV0262773.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILT"
BASE COUNT         4894 a         2799 c         3554 g         4737 t
ORIGIN      
        1 tctgaaaatt taataaatta gaaagcgagt aaatttatgt cccgttccca attaacaatt
       61 ttaacaaata tctgtctgat tgaagacctc gaaactcagc gcgtggtgat gcagtatcgc
      121 gcccctgaaa acaatcgctg gtctggttat gcctttcctg gaggtcatgt agaaaatgat
      181 gaggcttttg cggagtctgt cattcgtgaa atctacgaag aaacagggtt gactatccaa
      241 aatcctcaac ttgtcggcat taaaaattgg ccactagata caggtgggcg ctatattgtc
      301 atttgttata aggcgactga gttctctggt acccttcaat cttcagaaga gggagaagtt
      361 tcttgggtgc aaaaagacca gattccaaac ttaaatctgg cctatgatat gctaccattg
      421 atggaaatga tggaagctcc cgacaagtca gagtttttct accctcgccg tacagaagac
      481 gattgggaaa agaaaatctt ctagtctttt actaaataac ctagctgatc caaggcctcc
      541 tcgatatagt ggaggtcttg ttgtgtttcg gcttcgacta ggtggtaatg gatgccatct
      601 gtcaattcag atagaggtct aaagtcagaa tgctcgactt gttctagaaa atgttgcaca
      661 tctctgcgac aagacagttt aagcaaggtt tcaatttctc cataaacggg atggtcaatc
      721 aaggtattct ggacatgccc accattatcg acaatagcaa ggagttcctg accgatttct
      781 tcaacttcat gtttcacttt gaaaagtttg tgaacataag gattggtatc aatttgctta
      841 tagatgtagc cacgattggt ggataggata ggagcgccat cggctctcaa aattgcaatg
      901 tcctgcacaa tgacctgacg tgtgacatga aaatgttccg ccaaactttg gccattaagg
      961 gctttaggag cttctttcaa gagttggagc agggcttgtt tgcgatcttt tgtcatagct
     1021 tttcctttta acggcgtttt cgaagcactt tatagacagc tagtgctaat gtatagtcta
     1081 ccatactatg gataattgtg ccaaatccaa ctagtacaaa tagaacatag aacatatttt
     1141 ctacattggt acccgaagtt gcgtaaaaaa ggacacaggc taatacttca gcaagggcat
     1201 gaacaacagc caaaacaaaa ttgaaaaccc aggaagattt tggtttatct agggtatcgg
     1261 ggaatttttg taggtaaaga gctcctaaag caccaaaaga tatatgggaa aaagcccgaa
     1321 aaacgataac catgggatag ccagccatca aaaatccaaa actagaggct aggatgacaa
     1381 aaactgccat caagggcgac aagaacatgg ctataaaaat agcgatgtgg ctccccaaag
     1441 tataggaagc aggtggaatg acaatcttga aaggcataac aattggaatc aaaatcgcaa
     1501 tagccgttaa aagggctgtc attgtcataa attgtgtctt tttccgtgta ttcacaagaa
     1561 tctccttttt aactgcatat acactagtat ggtacaataa accagacaat aaagcaagaa
     1621 tttacttggg tttatagatc attttttagt taaaagttat agtagatgga aactagaata
     1681 gtccacctct acttctaaaa cattgttagc gatttgactg tcctgatcga tttgtcctgt
     1741 tcttatttcg ttttactata gtaaagattt cattaaaaag aaactgtata gagcaaaatc
     1801 tccaccttca ggtttgaaag cggagattgt ttttattttt tcaaggtttg tagtcgtgga
     1861 acaatggcta gggttagaat tgcagaaatg accaattcag caattgaatt tgttgagata
     1921 acggttgcca gaagaagttg gatatttcca ttataaacat ttccaaatag gaagaagatt
     1981 cctccaagga caaagatagt atttgtcaag gaaccaaggg ctccagctaa aatcagacca
     2041 gtcttgtttt tcatcagttt atagactaag taaggagtta aaccaatcaa aatacgtggg
     2101 acgatggcaa tgatagctga gtagatgttt ccgtttggta cgaagggaga gaagaggtag
     2161 cttgtcggta gaatcgtaat cgtgttaacc gtcaagctaa gtaatcccat caaaaatcca
     2221 agtgtaaccc caactcgtgg accataaata atgctggcaa taatgacagg aatatgaaca
     2281 atggtcggtt tgattggaaa tggaaaaagg ttaaagataa gtgagctcag aaagtgtatc
     2341 acgagcatgg tagcaaaaaa gatagcaatg ggtgcaatat tagagcgttt tttcatcgat
     2401 agtttccttt attctttcta aaataattgt gaggtcagct aaagctcctc gtccgtggtc
     2461 tccacaagct agtagggatt ccttaggagc aatcagctga tagccgtagg tttctaatgt
     2521 tttcagatta ttctgagtta ctggatggtc atacattttt gtattcatag caggagctat
     2581 tagtttggga atatgacttg gtagggctag agctgtactg gttaccatgt tgtccgcaaa
     2641 tccgtgagct agttttgcaa tagtgttagc agttgcaggt accacgataa ataaatctgc
     2701 tttttttcca agttcgatat gattgacctg atcaggatag ggttccttca tgacatccaa
     2761 gtggacagga ttctgtgaga gtacctgtag tgtcaaaggt tggataaact ctgtagcagc
     2821 ctgagtcatt aagacagtga cttgatggcc ttgttttttt agagaactga ctaaatctgc
     2881 cgacttataa gaggcgattg aacccgttac agccaagaga atgtttgcca tagttttcct
     2941 ttctaagaat gataggcttg aattttttca aggaggagtt ctgcaatttc ttctttagtc
     3001 tggactgttt gaagctgatt tttctcaaca aatatagctc ggtgctgatc tgctgaaatt
     3061 tgagtcaggt cattcgcgat gattaaatct gcttgattct tgataagact ttttcgtgca
     3121 atgtcaacca gatgatcttc ggtaacatca accagcagtt tgaaaccaat cagatgaata
     3181 gtgggattcc attccttgac tagggatatg attttgggtg tctttttaag gaacaaaacc
     3241 tgaacctcat cagttgaaga aatcttggcc tgatgattct gcttgcttaa aaattctttt
     3301 agattggagc tagcctgaac ttcctcaagc cctgtcatat aaacaggagt gtagtcagaa
     3361 acagccattg agtggatcaa gacctgataa tcctgaacac gttcttgcat ttctattaga
     3421 aggtccttgg tattggtaat ttctcgaata cttaggttag gatgaggctc tggcttcaga
     3481 gctcgttttg tcgtaattaa acaaacttca tgccctgcag aaagcaaagt ctctgtgata
     3541 attttcccca agtgacctgt agaatggtta gtgatagagc ggacgctatc gatagcttca
     3601 ctggtaccgc ccgatgtaac taaaattttc atagcactat tgtacacaaa aataagcggt
     3661 aagtaaaatg aaagtgataa atttttggta aaaacgaaga tttactatag tttcaaacta
     3721 taaagccata taaaatttaa gaaaggactc taaaagcctt aaaaacagag atatttacga
     3781 acgtttagag agttttagga tgttaaaaat cttgttttgt gatataatga attatcatat
     3841 aagaggttag aggaattttg aatgaaaaca gatattgaaa tcgcacagag tattgagttg
     3901 aagccaattg ttgatgttgt agagaaactt ggtatttctt atgacgattt ggagttgtac
     3961 ggaaagtaca aggctaaact cagctttgat aaaattcgtg cagttgagag caatccagtc
     4021 ggtaaattga tcttggttac tgccatcaac ccaacacctg caggtgaagg aaagtcgacg
     4081 cttaccattg gtcttgcgga tgccttgaac aagattggca agaaaaccat gattgctatc
     4141 cgcgaaccgt ctcttggtcc agtcatgggg atcaagggtg gtgctgctgg tggtgggtat
     4201 gcacaagttc tgccaatgga agacatcaac ctccacttta ctggagatat gcatgctatt
     4261 acaactgcca acaatgccct ttctgccttg attgacaacc acttgcacca agggaatgag
     4321 ctggaaattg atcaacgtcg tatcctctgg gaacgcgttg tggacttgaa cgaccgtgcg
     4381 ctccgccatg tgactgttgg tcttggtggt cctctaaacg gtattccacg tgaggatggt
     4441 tttgatatta cagttgcttc agaaatcatg gcaattcttt gcttggcaac ggacatcgag
     4501 gacttgaaac gtcgtttggc gaatatcgtt attggttatc gctatgaccg tacgcctgtt
     4561 tctgtaggtg atttgcaggt tgagggtgcc ttggctttga ttttgaagga tgctattaag
     4621 ccaaacttgg ttcagacaat ttacggtaca cctgcctttg tacacggtgg tccatttgcc
     4681 aatatcgctc atggctgtaa ctctgttttg gcgacgacaa cagcccttca cttggctgat
     4741 tacactgtta ctgaagctgg ttttggtgcg gaccttggtg ctgagaaatt ccttgatatc
     4801 aagacaccaa acttgccaac atctccagat gcagttgtca ttgtcgcaac ccttcgtgcc
     4861 cttaagatga atggtggtgt ggctaaagac gctctgactg aagaaaatgt agaggcagtt
     4921 cgtgcaggtt ttgctaactt gaaacgccac gttgaaaata tccgtaagtt cggtattcca
     4981 gcagttgtag ctattaacga atttgtatct gatacagaag ctgaaattgc agtcttaaaa
     5041 gaactctgtg cctcaatcga tgtaccagtt gaattggcta gtgtctgggc tgatggagca
     5101 gaaggtggag tagcacttgc cgaaacagtt gttaagacaa ttgctgaaaa tccagctaac
     5161 tataaacgtt tgtacgataa tgccctttct gtccaagaaa agattgaaaa aattgtcact
     5221 gaaatctatc gtggtagcaa agtgaacttt gagaagaaag ctcaaacaca aattgctcaa
     5281 atcgttcaaa acggttggga caaattgcca atctgtatgg ctaaaactca atacagtttc
     5341 tcagacaatc caaatgcgct tggagcacca gaaaactttg aaattaccat tcgtgaattg
     5401 gtaccaaaat taggtgcagg cttcatcgtt gccttaactg gtgatgttat gaccatgcca
     5461 ggtcttccaa aacgtccagc agctctcaac atggatgttg aaagcgatgg aactgtacta
     5521 ggcttgttct agtggattga aattgacttt aagtgagttt ggaaataata tccaagctca
     5581 ttttttctca gactgcaagc agttcatcaa taagatttat actcttcgaa aatctcttca
     5641 aactacgtca gcttcacctt gccgtaggta tatgatactg actctgtcag ttctatccac
     5701 aacctcaaaa cagtgttttg agcaacctgc ggctagcttc ctagtttgct ctttgatttt
     5761 cattgagtat aagtagatag aaaagttata tactatctat tgaaaatatt ataaatcaga
     5821 tacaaagaca ggtgaaacct tctgtgttat actagagata tgttagattt gaaagaatac
     5881 ggtatcgtca tgtggccgga ggagaaagtc atttctttcc gtgagaaact tctcgcttgg
     5941 tatgatgaaa acaaaagaga tttgccttgg aggagaagta aaaatcctta tcacatctgg
     6001 gtatctgaaa tcatgcttca gcagaccagg gtggatacag ttatccctta ctacgaaaga
     6061 ttcctggact ggtttccaac tgtcgaaagt ctggcaactg cgcctgagga gagtttactg
     6121 aaagcttggg agggcttggg ctattattct cgagttcgca atatgcaggc tgcagcccag
     6181 cagattatga ctgactttgg tggccaattt ccaaatacct atgaaggaat ttccagcttg
     6241 aaagggattg gaccttacac agcaggagcc atttccagta ttgcttttaa cttgcctgag
     6301 ccagctgtag atggtaatgt catgcgggtc ttggcgcgtc tgtttgaagt caaccacgat
     6361 attgggattc caagtaatcg aaaaattttt caggcaatga tggagatctt gattaatccg
     6421 gatcgtccgg gtgattttaa tcaagccttg atggacttag ggtctgatat tgagtctcct
     6481 gtaaatccca gacctgaaga aagcccagtc aaggacttta gtgcggcata tcagaatgga
     6541 accatggacc gttatccaat taaatctcct aagaaaaagc cggttcctat ttatcttaaa
     6601 gccttagttg tgaaaaattc tcaaggacaa tttttacttg aaaaaaatga aagcgaaaag
     6661 ctcttggcag gtttttggca tttccccttt atagaagttg ataacttttc gcaagaagag
     6721 cagtttgacc tctttcatca ggttgcggaa gaaagtgtga actttggccc cagtccagaa
     6781 gagagtttcc agcaggacta tgacctagat gttgattggc ttgatgtttg ttttgataca
     6841 gtccagcatg tttttagtca tcgaaaatgg catgtacaga ttgtagcagg tcaggtgagt
     6901 gacttccatg atttttcaga tagggaagtt cgttggcttt caccagaaga attcaagaat
     6961 tacccacttg ccaaacccca acaaaaaatc tggcaggctt atgcacaagc caacttagac
     7021 agtagccaag actagctatt gtgtcttctg actatttttt ggtataatag tagagaaaaa
     7081 ggtgaacata tgaaaaaaat actaattgta gatgatgaga aaccaatctc ggatattatc
     7141 aagtttaata tgaccaagga aggttacgaa gttgtaactg cttttaatgg tcgtgaagcg
     7201 ctagagcaat ttgaagcaga gcaaccagat attattattc tggatttgat gcttccagaa
     7261 attgatggtt tagaagttgc taagaccatt cgtaagacaa gcagtgtgcc cattcttatg
     7321 ctttcagcca aagatagtga atttgataag gttatcggtt tggaacttgg ggcagatgac
     7381 tatgtaacga aacccttctc caatcgtgag ttgcaggcgc gtgttaaagc tcttctgcgt
     7441 cgttctcaac ctatgccagt agatggtcag gaagcagata gtaaacctca atctatccaa
     7501 attggggatt tagaaattgt tccagacgcc tacgtggcta aaaaatatgg cgaagaacta
     7561 gacttaaccc atcgtgaatt tgagcttttg tatcatttag catcgcatac aggtcaagtc
     7621 atcacgcgcg aacacttgct tgagactgtc tggggttatg actattttgg tgatgtccgt
     7681 acagttgatg tgactgtacg acgtctgcgt gagaagattg aagatacgcc cagccgacca
     7741 gagtatatct tgacgcgccg tggtgtaggg tattacatga gaaataatgc ttgatttact
     7801 gaaacaaacc atttttacca gagattttat ctttatcctg attttgttag gtttcatcct
     7861 tgttgtgacc ctcttattac tggaaaatag acgtgataat attcagttga agcaaatcaa
     7921 tcaaaaggtt aaagatttga ttgcaggaga ttattccaag gttcttgata tgcaaggtgg
     7981 gtctgaaatc accaatatta ccaataattt gaatgacttg tcggaggtta ttcgtctcac
     8041 tcaggaaaat ctagaacaag agagtaagag gctaaatagt attctgtttt atatgacaga
     8101 tggggttctt gcgaccaacc gtcggggtca gattatcatg attaacgata cagccaagaa
     8161 gcaactgggg ttggttaagg aagatgttct gaatagaagc attttggaat tgctcaagat
     8221 agaagaaaac tatgaattgc gtgatttgat tacccaaagt ccagaattgt tgctagattc
     8281 ccaagatatc aatggcgaat atttgaacct tcgagttcgc tttgccttga tacgtcgaga
     8341 gtctggcttt atttcaggtt tggtggctgt tttgcatgat acgacggagc aggagaagga
     8401 agaacgcgaa cgaagactct ttgtttccaa tgttagccat gagttacgga ctcctctgac
     8461 tagcgtaaaa tcctatcttg aagccttgga tgagggggct ttgtgtgaaa ctgtagcacc
     8521 agactttatc aaggtttctc ttgatgagac caaccgtatg atgcgcatgg tgacggatct
     8581 cctccatctt tcacgtattg ataatgctac cagtcaccta gatgtggaac tgattaactt
     8641 cactgctttt attaccttta tcctcaatcg ttttgacaag atgaaaggac aggaaaagga
     8701 gaaaaaatat gagttggtga gagattatcc catcaattct atctggatgg aaattgatac
     8761 agataagatg acgcaggttg tcgacaatat tttaaataat gctattaagt attcgccaga
     8821 tgggggtaaa atcactgtca gaatgaagac aactgaagac cagatgattt tatccatttc
     8881 tgaccacggt ttggggattc ctaagcagga tttaccacgt atctttgacc gtttctatcg
     8941 tgtggatcgt gctagaagtc gtgcacaagg tggtacaggt ctaggactgt ctatcgctaa
     9001 agaaattatc aaacaacata agggctttat ttgggccaag agtgaatacg gcaagggttc
     9061 aacctttacc attgtgctcc cttatgataa ggatgcagtg aaagaagaag tatgggagga
     9121 tgaagtagaa gactagaatg agtgaaatag gctttaaata cagtatttta gcgtcgggtt
     9181 ccagtggaaa ttctttttat ctggaaacct caaaaaagaa gcttttagta gatgcaggct
     9241 tgtctggcaa gaaaattacc agtctgctag ctgaaattaa ccgtaagcca gaagacctgg
     9301 atgccatctt gattacccat gagcattcag atcatatcca tggagtaggc gttttggctc
     9361 gcaagtatgg tatggatctt tatgccaatg aaaagacctg gcaagctatg gaaaatagta
     9421 aatatcttgg caaggtggat tcttcgcaaa agcatatttt tgaaatgggt aaaaccaaaa
     9481 cctttggaga tatcgacatc gagagttttg gtgtaagcca tgatgcagtc gcaccgcagt
     9541 tctatcgctt tatgaaggat gataagagtt ttgtcctttt gacagataca ggttatgtca
     9601 gtgaccgtat ggcgggcatt gtcgaaaatg cggatggcta tcttatcgag gccaaccatg
     9661 atgtagagat tttgcgatca ggttcttacg cttggcgact caaacaacga atcctatctg
     9721 accttggtca cctttctaac gaggacggtg ctgaagctat gattcggacg ctaggaaatc
     9781 gtactaagaa gatttacctt gggcatttat ctaaggaaaa taatatcaag gaactggctc
     9841 atatgaccat ggtcaatcag ctggctcaag ctgatctggg agtcggagta gactttaagg
     9901 tttatgatac ctcaccagat accgcaacac cattgacaga gatatagaaa gaacgctgag
     9961 aaggtgttct ttttatattg actgaacacc taaaaagtaa tacaatggtg ttaccattaa
    10021 aaaagggagc acaaaagatg actactatta cattaaaagt ttctgaagct gataaaacat
    10081 ttatgaaagc aatggctaag tttgaaggag tttccctttc ggaacttatt cgcaccaaaa
    10141 ctcttgaagc cctagaagat gaatacgatg ctcgtgtggc agatttagcc tatcaagagt
    10201 atttagaaga cttggaaaaa ggagttgaac ccattacttg ggaagaaatg atgcatgatt
    10261 taggcttgaa ggatgaataa tttgtataaa ttagttccaa caagacgttt tatcaagcaa
    10321 ttgaaaaaat tggaccgtta tacgcagaag ctaattacaa actatttaca aaccaatgtt
    10381 ttggaaggtc caagacgaca cggaaaggct ttggttggta atcgcgttgg tcaatggcgc
    10441 tatagaattg gtaattatcg agttatcgta caaattgtag atgatgaatt agtcgttgct
    10501 actctagaag ttggtcatcg gagagatatt tattgaatta aagctgagta ataacaatat
    10561 agaaagtaag gagagtaaaa atttgaagaa aaaatggtta tttgcagatt attatgatac
    10621 aacaattatt ttacttgctt taatctctgt tattctggta ttacttggat ttgctgagat
    10681 gactgatttg gacaatccgc cttatagtat tattgattta gtaatctggg gtgtttttgt
    10741 aattgattat agttggcgct tttttattac taaaagaaaa tggcgcttta ttcttgaaaa
    10801 tatttttgat ttattgacca ttctcccttt aaatgctatt tttacagtgt ttcgattagg
    10861 acgtatcttc cgcttagcaa ggctgacaaa attactgaaa ctgactcgtt tacttagaat
    10921 aattggactt acaggtaaac tagaaagaaa aattagtaga ttcttacgaa caaatggatt
    10981 gatttatatc ttgtatgtta atatctttat tgttctagta gggagttcga ttttatccgt
    11041 tgttgaagaa aaatccttct cagatagtct ttggtgggct cttgtgacag taactactgt
    11101 tggttatggt gatattgttc cagtttccct ttttgggaaa tggctagcag ttttactgat
    11161 gcttgtcggt attggaacaa tagggatgct aacaagtgct ttgacgaact tttttgtaaa
    11221 ggataatcca gatgaacaga taaaacttga taaactcaaa gacgaattat cgagctaaag
    11281 aatattaata gagaaacaat ctgaaaaaat cgaagaacta cattgaatga taaaagactt
    11341 agttgaaaaa atatcataaa ggtgagacaa aataaaactt gattcgagga gaaatcaagt
    11401 aagttttgac acaatagaac gcatattatc aggtataaaa acttgatact atgcgtttta
    11461 tagagaaata aaataaaatc tgaacaaatc aattaggaat ttaaagcatt ttataacaac
    11521 gttttagagt aatggggtgc tatttcaact tcaacctact ataatacaga aaaaaataca
    11581 gaaaaaaaca actccctgat gattcaagga gttgtctata gttaaattag tttttagaag
    11641 cttcttggaa ttctggattt ttccatgctt cgtcaataat agcttgcaat tctttagcag
    11701 atgcttgcat tttttgagtt tctgcatcgt tcaatggaat gtttactgga cgaacgatac
    11761 catgtgcacc aacaacagct ggttgaccga taaagacatt ctcaactccg tattgacctt
    11821 cttggaatac tgaaagtgga agtactgcgt tttcatcgtc aaggattgct ttagtgatac
    11881 gagcaagggc tactgcgata ccgtagtatg ttgcaccttt tttgttgatg attgtgtagg
    11941 ctgcatcacg aacaccttcg aacaattcaa tcaattcagc ttcttgaaca ttttgagtgt
    12001 ctttaaggaa ttcttcaagg tttacaccag cgatgttagc gtgtgaccaa acagcgaact
    12061 cagagtcacc gtgttcaccc atgatgtagg cgtgcactga acgagcatcc acatccaatt
    12121 tttcagcaag tgcttgacgg aaacgagctg agtcaagtga agtacctgaa ccgataacgc
    12181 gttctttagg gaaaccagag aatttccaag ttgagtaagt caaaacgtca actgggttag
    12241 cagcaacaag gaagatacct ttgaaaccag attcaacaac ttgagttacg attgatttgt
    12301 tgatagcaag gtttttacct acaaggtcaa gacgagtttc acctggtttt tgaggtgcac
    12361 ctgcagtgat cacaacaagg tcagcgtctg cacagtcaga gtattgagct gcatagattt
    12421 ttttaggtga agtgaaggca agggcgtgac taaggtcaag cgcatcacca acagcttttt
    12481 catgcaattg tggaatttcg ataattccaa gctcttgtgc aattccttgg ttaacaagtg
    12541 caaaagcgta agatgaacct acagcaccat caccgacaag gataactttt ttgtgttgtt
    12601 tagttgaagt cattgtttta aacatctcct taattttatt aggggatttt ccctagacaa
    12661 cttcattcta tcacttttaa aaaactttgt cacgaatatg ccttatagtt ctcgatgtaa
    12721 acgttttagt ggtttagagg ctgaaataga tgggaattta tggtataatg ttgttactta
    12781 ctaattgtga aatgaggcat ttattaatgc aggataaaaa tttagtgaat gtcaatctga
    12841 caaaggagat gaaggcaagt tttatcgact acgccatgag tgttatcgta gcgcgagctc
    12901 ttcctgatgt tcgagatggc ttaaaacctg ttcaccgtcg cattctctac ggaatgaatg
    12961 aattgggtgt gaccccagac aaaccccata aaaaatctgc tcgtattaca ggggatgtca
    13021 tgggtaaata tcacccacac ggggattcct ctatttatga agccatggtc cgtatggctc
    13081 aatggtggag ctaccgttac atgcttgtag atggtcatgg gaattttggt tccatggatg
    13141 gagatagtgc tgccgctcaa cgttataccg aggcacgtat gagcaagatt gctctggaaa
    13201 tgcttcgtga tatcaacaaa aatacagttg atttcgttga taactatgat gccaatgaac
    13261 gggaaccctt ggtcttgcca gcgcgttttc caaacctttt ggttaatgga gcaactggta
    13321 tcgcggttgg gatggcaacc aatattccac ctcataatct gggtgaaacc attgatgcag
    13381 tgaagttggt catggataat cctgaagtga ctaccaagga cttgatggaa gtcttgcctg
    13441 gaccagattt tccaactggt gctcttgtca tggggaaatc aggtatccat aaggcttatg
    13501 aaacaggtaa aggttcgatt gtcctacgtt ctcgtacaga gattgaaacg actaagactg
    13561 gtcgtgagcg tatcgttgta acagaatttc cttacatggt caataaaacc aaggtgcatg
    13621 agcatattgt tcgcttggtt caggaaaaac gcattgaggg tatcacagca gtacgtgatg
    13681 agtcaaaccg tgaaggtgtt cgatttgtta ttgaagtcaa gcgcgacgcc tcagccaatg
    13741 ttattctcaa taacctcttc aaaatgaccc aaatgcaaac caattttgga ttcaatatgc
    13801 tcgctatcca aaatggtata ccgaaaattt tgtctcttcg tcagattttg gatgcttata
    13861 ttgagcacca aaaagaagtg gttgttcgtc gtacacgttt tgataaggaa aaagcggaag
    13921 cgcgcgctca tatcttagaa ggtctcttga ttgcgctaga ccatatcgac gaagtgattc
    13981 gtatcatccg tgctagtgaa acggatgcgg aagctcaagc tgagttgatg agcaagttta
    14041 agctttctga acgtcaaagt caagctatcc ttgatatgcg tcttcgtcgt ttgacaggtt
    14101 tggaacgcga taagattcaa tctgagtatg atgacctctt ggctctgatt gcggatttag
    14161 cagatattct tgctaagcct gaacgtgttt ctcaaattat caaagacgaa ttggatgaag
    14221 ttaaacgtaa attttctgat aaacgccgta cagagttgat ggttggacag atcttgagtc
    14281 tcgaggatga ggacttgatt gaagaatcgg atgtcttgat taccctttct aacagaggct
    14341 acattaagcg tttggatcag gacgagttca ctgctcaaaa acgtgggggt cgtggtgtcc
    14401 aaggaacggg agtgaaagat gatgactttg ttcgtgagtt agtgtcaact agcacccatg
    14461 atcatctgct cttcttcaca aacaagggac gtgtctatcg tcttaaaggt tatgaaattc
    14521 ctgagtatgg tcggactgcc aaagggctac cagtagtcaa tctcttgaaa ttggatgaag
    14581 atgaaagtat tcagacggtt atcaatgttg agtctgatcg cagtgatgat gcttatctct
    14641 tctttacaac ccgtcacggt attgtgaaga gaactagtgt taaggagttt gccaatattc
    14701 gtcaaaatgg tctcaaagcg ctgaatttaa aggatgaaga tgagttaatc aatgtcttgt
    14761 tgacagaagg agatatggat attatcattg gtaccaagtt tggttatgca gttcgcttta
    14821 atcaatcagc cgttcgtggt atgagccgta tcgccactgg tgtgaaaggt gttaaccttc
    14881 gtgaaggaga cacagttgtt ggtgccagct tgattactga tcaagatgag gttcttatta
    14941 tcacagaaaa aggatatggt aagcgtacag tcgctactga atacccaaca aaaggtcgtg
    15001 gtggtaaggg aatgcagaca gctaaaatta ccgaaaaaaa tggcttgctg gccggtctta
    15061 tgactgttca aggggatgag gatttgatga ttatcactga tacaggtgtc atgattcgaa
    15121 ccaatcttgc caatatttca caaacaggac gtgcaactat gggagttaaa gtaatgcgcc
    15181 tggatcaaga tgctcagata gtgactttca caacggttgc ggtggcagaa aaagaagaag
    15241 ttgggacaga aaacgaaaca gaaggtgaag cataatgtct caaaaaaata ataaaaagaa
    15301 aaacaagcga aaaaatctgc tgacaaatat cctagcagga tttctgatat tactgtcact
    15361 ggctttgatt tttaatactc aaattcgaaa tattttcata gtctggaata ccaataagta
    15421 tcaagttagc caggtatcaa aagaaaaatt agaagaaaat caggatacag aaggcaattt
    15481 tgactttgat tctgtcaaag ctatctcttc ggaagctgtt ctaacttctc aatgggatgc
    15541 tcaaaaatta ccagttattg ggggaattgc aattcctgaa ttggaaatga atttgccgat
    15601 ttttaaagga cttgataatg ttaatctctt ctacggagct ggtacaatga aacgcgagca
    15661 agtaatggga gaaggaaatt atagtctagc tagtcaccat atctttggtg ttgataatgc
    15721 taataaaatg ttattttctc ctttagataa tgctaaaaat ggcatgaaga tttatctaac
    15781 cgataaaaat aaagtttata cttatgaaat acgtgaagtc aaacgtgtga caccggatcg
    15841 tgttgatgaa gttgatgata gagatggggt caatgaaatc acattagtaa cctgtgaaga
    15901 ccttgctgct acagaacgta ttattgtcaa aggtgatttg aaagaaacaa aagattattc
    15961 acaaacatct gatgaaattc taac
//
LOCUS       DAVKWZ010000046        15729 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000046 DAVKWZ010000000
VERSION     DAVKWZ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15729
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.046"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            93..1118
                     /gene="trpS"
                     /locus_tag="VW854_001560"
     CDS             93..1118
                     /gene="trpS"
                     /locus_tag="VW854_001560"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEV0262774.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            1270..2748
                     /gene="guaB"
                     /locus_tag="VW854_001561"
     CDS             1270..2748
                     /gene="guaB"
                     /locus_tag="VW854_001561"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEV0262775.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(2799..3896)
                     /gene="recF"
                     /locus_tag="VW854_001562"
     CDS             complement(2799..3896)
                     /gene="recF"
                     /locus_tag="VW854_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEV0262776.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(3899..4267)
                     /gene="yaaA"
                     /locus_tag="VW854_001563"
     CDS             complement(3899..4267)
                     /gene="yaaA"
                     /locus_tag="VW854_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEV0262777.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            4426..5676
                     /locus_tag="VW854_001564"
     CDS             4426..5676
                     /locus_tag="VW854_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV0262778.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYPSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKSWIAKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            5673..6956
                     /locus_tag="VW854_001565"
     CDS             5673..6956
                     /locus_tag="VW854_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV0262779.1"
                     /translation="MTKVVFEEKYYPAVKEKVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSIDTLVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLYLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            6998..7819
                     /locus_tag="VW854_001566"
     CDS             6998..7819
                     /locus_tag="VW854_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV0262780.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVTLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            7830..8375
                     /gene="pgsA"
                     /locus_tag="VW854_001567"
     CDS             7830..8375
                     /gene="pgsA"
                     /locus_tag="VW854_001567"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEV0262781.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILASAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            8372..9199
                     /locus_tag="VW854_001568"
     CDS             8372..9199
                     /locus_tag="VW854_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV0262782.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            9184..10023
                     /locus_tag="VW854_001569"
     CDS             9184..10023
                     /locus_tag="VW854_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV0262783.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            10016..10810
                     /locus_tag="VW854_001570"
     CDS             10016..10810
                     /locus_tag="VW854_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV0262784.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            10869..11687
                     /gene="mreC"
                     /locus_tag="VW854_001571"
     CDS             10869..11687
                     /gene="mreC"
                     /locus_tag="VW854_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEV0262785.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            11687..12181
                     /gene="mreD"
                     /locus_tag="VW854_001572"
     CDS             11687..12181
                     /gene="mreD"
                     /locus_tag="VW854_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEV0262786.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            12275..13453
                     /locus_tag="VW854_001573"
     CDS             12275..13453
                     /locus_tag="VW854_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEV0262787.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            13677..14456
                     /gene="rpsB"
                     /locus_tag="VW854_001574"
     CDS             13677..14456
                     /gene="rpsB"
                     /locus_tag="VW854_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEV0262788.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEAGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            14535..15575
                     /locus_tag="VW854_001575"
     CDS             14535..15575
                     /locus_tag="VW854_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEV0262789.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
BASE COUNT         4929 a         2483 c         3367 g         4950 t
ORIGIN      
        1 tgtaaaaagc aaggctgatt tattttgata aattacggtt atttcattaa aaaaatgcta
       61 taattgaaag gactatatcg aaggagaaca aaatgactaa acccattatt ttaacaggag
      121 accgtccaac aggaaaattg catattggac attatgttgg aagtctcaaa aatcgagtat
      181 tattacagga agaggataag tatgatatgt ttgtgttctt ggctgaccaa caagccttga
      241 cagatcatgc caaagatcct caaaccattg tagagtctat cggaaatgtg gctttggatt
      301 atcttgcagt tggattggat ccaaataagt caactatttt tattcaaagc cagattccag
      361 agttggctga gttgtctatg tattatatga atctagtttc gttagcacgt ttggagcgaa
      421 atccaacagt caagacagag atttctcaga aaggatttgg agaaagcatt ccgacaggat
      481 tcttggtcta tccaatcgct caagcagctg atatcacagc tttcaaggct aattatgttc
      541 ctgttgggac agatcagaaa ccaatgattg agcaaactcg tgaaattgtt cgttctttta
      601 acaatgcata taactgtgat gtcttggtag agccggaagg tatttatcca gaaaatgaga
      661 gagcagggcg tttgcctggt ttagatggaa atgctaaaat gtctaaatca ctaaataatg
      721 gtatttattt agctgatgat gcggatactt tgcgtaaaaa agtaatgagt atgtatacag
      781 atccagatca tatccgcgtt gaggatccag gtaagattga gggaaatatg gttttccatt
      841 atctagatgt ttttggtcgt ccagaagatg ctcaagaaat tgctgatatg aaagaacgtt
      901 atcaacgagg tggtcttggt gatgtgaaga ccaagcgtta tctacttgaa atattagaac
      961 gtgaactggg tcctattcgt gagcgccgta ttgaatttgc taaggatatg ggagaagttt
     1021 ataatatgat tcaaaaaggt agtgaaagag cgcgtgaagt tgcgggtcaa accctatctg
     1081 aggtaaaagg ggcaatggga cttcattact ttaactaagt ttattttaca agaaactatc
     1141 atttctatct cgaagaaaag atagtttttt atttacccct gtaacatttg acaaattttt
     1201 atagaatggt atgatagata caatataaaa agagtcaagc tcaaaaagaa agaaaagagg
     1261 aaacttcgaa tgtctaattg ggacactaaa tttttgaaaa aaggttttac ctttgatgat
     1321 gtattgctta ttccagctga aagtcatgtg ttgcctaacg atgcagattt aacaactaaa
     1381 ttggcagata atttgacttt aaatatccca attattaccg ctgccatgga cacagttaca
     1441 gagagtcaaa tggccattgc tattgctcgt gcaggtggtc tcggagttat ccataaaaac
     1501 atgtcaattg ctcaacaagc agacgaggtt cgtaaggtaa aacgttctga aaatggagtt
     1561 attattgatc cgttcttctt gacgcctgaa catacaattg ctgaagcaga tgagcttatg
     1621 ggtcgttacc gcatcagtgg tgttccagtt gttgaaacac ttgaaaatcg taaattggtt
     1681 ggtattttga caaaccgaga tcttcgtttt atttcagatt ataatcaacc aatttcaaac
     1741 catatgacta gtgaaaatct tgttactgct cctgtgggta cggatcttgc aacggctgag
     1801 agtattcttc aagagcatcg tattgaaaaa cttccgttgg ttgatgaaga aggcagtctt
     1861 tctggtttga tcactatcaa agatattgaa aaagttattg agtttccaaa tgcggctaaa
     1921 gatgagtttg gtcgtcttct agttgcaggt gcagtaggtg ttacttcaga tacatttgaa
     1981 cgtgcagagg ctctttttga ggcaggagcg gatgcgattg ttattgatac tgcacatggt
     2041 cattctgcag gtgtcttgcg taaaattgcc gagattcgtg ctcatttccc agatcggact
     2101 ttgattgctg gaaatattgc tactgctgaa ggtgcacgtg ccctttatga agcgggtgta
     2161 gacgttgtta aggttggtat tggaccaggt tctatctgta ctactcgtgt gattgctggt
     2221 gttggtgttc cgcaagtaac agctatctac gatgctgcag ctgttgcgcg cgaatatggt
     2281 aaaacgatta ttgctgacgg tgggatcaag tattctggag atattgtaaa agcacttgct
     2341 gcaggtggaa atgctgttat gcttggatct atgtttgctg gaactgatga agctccaggc
     2401 gaaactgaaa tcttccaagg acgtaaattc aagacttacc gtggtatggg atcaattgct
     2461 gctatgaaga aaggttcaag cgaccgttat ttccaaggtt ctgtcaatga agcaaacaag
     2521 cttgttccag aaggaattga aggtcgtgtt gcttataaag gagcggcagc tgatattgtt
     2581 ttccaaatga ttggtggtat tcgctctggt atgggttact gtggtgcagc taaccttaaa
     2641 gaactacacg ataatgctca atttattgaa atgtctggtg ctggtttgaa agaaagccat
     2701 cctcatgatg tgcaaattac taatgaggca ccaaattatt ctatgtaaaa aacaatgaaa
     2761 agaactccag tgaaaacagg agttctttta caatgttgtc aatttccatt tacagcagct
     2821 ttaccatcct gaatagtgaa gatacttaga ttttctggca gattttgaag atggtctaag
     2881 cttgttgttg tgataaaggt ttggattgat tgagaaatcg tttctaataa ttttaactgt
     2941 ctagtgttgt caagttcact catcacatcg tcaagcagta atataggaga ttctgtggta
     3001 atgctttcca ttaattcgat ttctgctaat tttatcgaga ggacgagact acgatgttga
     3061 ccttggcttc cgaaactagc atccatccca tttatataaa aagaaatgtc atctcgatga
     3121 ggaccgacac cagtattctt tttaaataaa tctctggatc tacttttttc taaagcaatt
     3181 ttgaaagatt cggataagtt ttgtttgtca gttatattga cagaagattg ataggatatt
     3241 gacaactctt cgatctgatt agagagttca aaatgtttct tacgcccaaa tgattctagt
     3301 ttttttatga aatctaagcg gtgattcatt acacgacatc cataatcaac tagctgatca
     3361 tctaacacag aaaggaatgt ttcatctatt ttttgagctg attttaggta agtgtttctt
     3421 tgctttagga tgtggttata attggttaag tcagataaat agattggctt aatttgccca
     3481 agttccatat caatgaattt tcgtcgaatc gaaggtgctc ctttaattag ttgtaaatct
     3541 tcaggagcaa ataagacaac attcatgtgt cctacataat ctgaaaggcg tgcctgtttt
     3601 aagtgattaa cttttgttac acggcctttt tgtgttagtt cgatttctag gggaatggat
     3661 ccagtttttt tctgaacgag acctgaaaga tgaagttgtt cctcatcaaa atgaatgaga
     3721 tttttatctg ttcgagttcg atgactacgc gttaaggcta aaaaatagat agcctctaac
     3781 atgtttgttt ttccttgtgc attacgtccc aaaaagacat tcaatttagg attaaagtct
     3841 attttcgttt ctttgtaatt acgaaaagtc ttgagagata gatgttgtag ccacatgatt
     3901 atctaccagg gaatcgtgga gcttgttggc ttttaggtga tgaagcaggt ttcgatttgt
     3961 cttttttaac tcccttattc atctttttga caagtttagc gatccgttct ttttcaactt
     4021 tatcagcttg gtattcatct tgttcttcag aagtaggttg tgtcaacaag atgtcaatgt
     4081 tcatgtcagg gatgtcaact ttatcgccaa tacgaagttt ttttccacga cgactctcta
     4141 attccctatt aaagtaaaca gaatgttcag agagaaatga tttaatagct cctccgctat
     4201 gtgtaattcc aagttctttg agtagtgctt ggagggtaat aaattcttca aataatttgt
     4261 attccataat tcacctcaat ctttggtatt ataccatatt ttcctaaaaa tagtgagaga
     4321 gacagcgaga aatgtaagcg atttttattt caaaagtgtt acagggaaca agaaaatttc
     4381 aggttttcgt gatataatag aagtctgtat ataaggaggt aaatcatgga gttagtgcat
     4441 ggaatttcaa cacattttat ccaatcaaaa aagtttaaaa caaacaaaat taccgtgcgt
     4501 tttaccgctc cattatccct tgatacgatt gcaggtcaca tgttgagtgc aagtatgcta
     4561 gagactgcta atcagatgta ccccacttct caagatttga ggagacactt ggccagtcta
     4621 tacggtacag atatgtcaac caattgtttc agaagagggc aaagccacat tatagaattg
     4681 acatttacct atgttcgtga tgagttttta agtaggaaaa acgtgctaac ctctcagatt
     4741 ttggaacttg taaaagaaac tcttttttca cccgtagtag ttgataatgg gtttgatccg
     4801 gccttatttg aaattgagaa aaaacaattg ctagcaagtt tagcagctga tatggatgat
     4861 tctttttatt ttgcacataa agaattggat aaattgtttt ttcatgatga acgtcttcaa
     4921 ttggaatata gtgatttacg aaatcgtatt ttagctgaaa ctccacaaag ttcttattct
     4981 tgtttccaag aatttttagc caatgatcga atagatttct ttttcctagg tgattttaat
     5041 gaggttgaaa ttcaaaatgt attagaatca tttggcttta aaggtcgaaa aggagatgtg
     5101 aaggttcagt attgtcaacc ttattctaat atccttcagg aaggtatggt tcggaaaaat
     5161 gtgggacaat ctattttgga attaggttat cattaccctt ctaaatatgg tgatgagcaa
     5221 catttaccca tgattgtaat gaatggttta cttggtggat ttgctcactc taagctcttt
     5281 acaaatgtcc gtgaaaatgc tggattagct tataccattt caagtgagct tgatttattt
     5341 agtggattct tgaggatgta tgctggtatc aatcgagaaa atcgtaacca ggctcgtaaa
     5401 atgatgaata atcaactgct tgatttaaaa aaaggttatt ttacagagtt tgagttaaat
     5461 cagaccaagg aaatgattcg ttggtcgttg ttactttctc aagataatca atcttcattg
     5521 attgaacgtg cttatcaaaa tgccttattt ggaaaatctt cagcagactt taaaagttgg
     5581 attgcaaagc ttgaacagat tgacaaagat gctatttgta gagtagctaa taatgtgaaa
     5641 ctacaagcga tttactttat ggaaggaata gaatgacaaa ggttgttttt gaagaaaaat
     5701 actatccagc tgtaaaagaa aaggtttatc gaactcgttt ggccaacgga ttgacagttg
     5761 ctcttttgcc taaaaaggaa tttaaagagg tttacgggag tgtaactgta cagtttggtt
     5821 cgatagatac gcttgtcaca gaagttgacg gagatgtaaa acaatatcct ggaggaattg
     5881 ctcattttct tgaacataaa ttatttgaga gagaagattc tagtgatttg atgtcggctt
     5941 ttacgagtct aggtgcagat agtaatgcct ttacaagctt tacaaaaaca aactatcttt
     6001 tttcagcaac ggattatttt ttagaaaatt tatatttact tgatgaattg gtaacatcag
     6061 cacactttac tgaagcttcc attctgacag agcaggatat tattcagcaa gaacgagaaa
     6121 tgtaccaaga tgatccagat tcgtgtttat tcttttcaac tttagcgaat ttgtatcctg
     6181 gtacaccttt agcaactgat atagttggaa gtgaggagtc catttcccaa atcaatttaa
     6241 ctaatttgca agaaaatttt acaaagtttt acaaacctgt aaacatgtct ctgtttttag
     6301 ttggtaattt tgatgtggag cgagtacagg actattttga aagcaaagaa ctgaaagatt
     6361 cagattttca ggaagtagca agagaaaagt tgtttttaca gcctgtaaag ccaacagata
     6421 gtatgagaat ggaagtatct tctcccaaac tagcgattgg agttagaggt aagcgagaag
     6481 tttctgaagc ggattgctat cgacatcata ttttattaaa attattgttt gcaatgatgt
     6541 ttggttggac ttcggatcgt tttcaaaaat gttatgaatc aggtaaaatt gatgcgtcct
     6601 tatctctgga agttgaaata acaagtcgct ttcattttgt catgttgaca atggatacga
     6661 aagagccagt tgctttgtct catcaattta ggaaggctat tcgtaatttt acaaaggatt
     6721 tagatattac agaggaacat ttagatatta tcaaaagaga gatgtttggc gaatttttca
     6781 gtagcatgaa ctctcttgaa tttattgcaa cgcaatatga tgcttttgaa aatggtgaga
     6841 caatttttga tttgccgaaa attttacagg aaattacttt agaggatgtc cttgatgctg
     6901 gacatcattt aatagatgat ggtgacatag ttgattttac aatattccca tcgtagtaac
     6961 ctatcataat agacactaga aagaagggat gacaagtatg agaaaaaaaa caattggaga
     7021 ggttttacga ttagctagaa tcaatcaggg attgagttta gatgaattgc agaaaaagac
     7081 agaaatccag ttagatatgt tggaagcaat ggaagcagac gatttcgatc aacttccaag
     7141 tcctttttac acgcgttctt tcttgaaaaa atatgcatgg gctgttgagt tagatgacca
     7201 aattgttttg gatgcttatg attctgggag tatgattact tatgaggaag tagatgttga
     7261 tgaagatgag ttgacaggtc gtagacgttc aagtaagaaa aagaagaaaa aaacatcatt
     7321 tttaccttta ttttatttta tcctttttgc tttatcgatt ttaatttttg tgacttatta
     7381 tgtttggaac tatattcaaa ctcaaccaga ggagccttct ctttctaatt acagtgtggt
     7441 tcaatcaaca agttcaacta gctctgttcc ccactcctca agtagtagtt cttctagtat
     7501 agaatcagct ataagtgtat caggcgaagg aaatcatgta gaaatcgctt ataagacaag
     7561 taaggaaaca gttaaattgc aattggcagt ttcagatgtt acaagttggg tcagtgtttc
     7621 agaaagcgaa cttgagggcg gtgtaacctt atcgccaaag aagaaaagtg cagaagcaac
     7681 agttgcaact aaaagtcctg taacaattac gttaggtgtt gtaaaaggtg ttactttgac
     7741 agtagataat cagactgttg atttatcgaa attaacagct cagactggac aaatcactgt
     7801 aacctttact aaaaattaag gaaaaacgaa tgaaaaaaga acaaattccc aatctcttaa
     7861 caataggtcg aattctcttt atacctattt ttatctttat tttaacgata ggaaattcga
     7921 tagagagtca tatagttgca gctattatct ttgctgttgc cagtattacc gactatttag
     7981 atggatattt agctcgtaaa tggaatgtgg tcagtaattt tggtaaattt gcagatccta
     8041 tggcggataa gttactagtt atgtcggctt ttattatgtt gattgagtta ggtatggctc
     8101 cggcttggat tgttgcagtg attatctgtc gtgagttagc tgtgacaggt ttaaggcttt
     8161 tattggttga aactggtgga acaattttag catcagcaat gcctggaaaa attaaaactt
     8221 ttagtcagat gtttgctatt attttcttgc tattacattg gactttgctt ggtcaagttc
     8281 tactttatgt agccttattt ttcactatct actctggcta tgactatttc aagggtagtg
     8341 cctatgtatt taaagggaca tttggttcga aatgaaatca ataattgatg taaaaaatct
     8401 ttcttttcgc tataaagaaa atcagaacta ctacgatgtg aaggatatta cgtttcacgt
     8461 gaaacgtgga gaatggcttt cgattgtagg gcataatggt agtggtaaat caactacggt
     8521 tcgattaatt gatggcttac tggaagcaga atccggagag attgtaattg atggccaacg
     8581 tttgactgag gaaaatgttt ggaatatacg tcgtcaaatc ggtatggttt ttcaaaatcc
     8641 agacaatcaa tttgttggag cgactgttga agatgatgtt gcttttggtt tggaaaatca
     8701 gggactttct cgtcaagaaa tgaaaaagag agtggaagaa gctctggctt tagttggcat
     8761 gttggacttt aaaaagagag agccagcgcg tctatcaggt ggccaaaagc aacgtgtggc
     8821 cattgcaggt gttgtagccc taagaccagc tattttaatc ttagatgaag caacgagtat
     8881 gttggatcct gaggggcgta gagaacttat tgggacagta aaaggaattc gaaaagacta
     8941 tgatatgacg gtcatttcta ttacccatga tttggaagaa gtcgccatga gtgatcgcgt
     9001 attggtcatg aaaaaagggg aaattgaatc aactagtagt ccaagggagc ttttctctcg
     9061 aaatgattta gatcaaattg gattagacga tccttttgct aatcaattaa aaaaatcttt
     9121 gagccagaat ggctatgatt tacctgaaaa ttatttgaca gaaagtgagc tagaggataa
     9181 gctatgggaa ttgctctaga aaatgtgaat tttacatatc aagaaggtac tcccttagct
     9241 tcagcagctt tgtcggatgt ttctttgacg attgaagatg gctcttatac agctttaatt
     9301 gggcacacag gtagtggtaa atcaactatt ttacaactct taaatggttt attggtgcca
     9361 agtcaaggga gtgtgagggt ttttgatacc ttaatcacct cgacttctaa aaataaagat
     9421 attcgtcaaa ttagaaaaca ggttggcttg gtatttcagt ttgctgaaaa tcagattttt
     9481 gaagaaacgg ttttgaagga cgttgctttt ggaccgcaaa attttggagt ttctgaagaa
     9541 gatgctgtga agactgcgcg tgagaaactg gctctggttg gaattgatga atcacttttt
     9601 gatcgtagtc cgtttgagct gtcaggggga caaatgagac gtgttgccat tgcaggcata
     9661 cttgccatgg agccagctat attagtctta gatgagccaa cagctggtct agatcctcta
     9721 gggagaaaag agttgatgac cctgttcaaa aaactccacc agtcagggat gaccatcgtc
     9781 ttggtaacgc atttgatgga tgatgttgct gaatatgcaa atcaagtcta tgtaatggaa
     9841 aagggacgtt tagtaaaggg gggcaaacca agtgatgtct ttcaagacgt tgtttttatg
     9901 gaagaagttc agttgggagt acctaaaatt acggcctttt gtaaacgatt ggctgataga
     9961 ggcgtgtcat ttaaacgatt accgattaag atagaggagt tcaaggagtc gctaaatgga
    10021 tagtatgatt ttggggcgtt atatcccagg ggattcgatt gttcaccgat tggatccacg
    10081 tagcaaattg cttgctatga tgctactgat tttgattgtg ttttgggcta ataacccctt
    10141 gacgaatctg attcttttta tagcgacagg gatatttatt gccttgtcag gagtatctct
    10201 ttcatttttt attcagggct tgaagtctat gtttttcttg attgccttca caactatttt
    10261 tcaactattt ttcatttcta atgggaatgt tttatttgag ttttcgtttg tgagaatcac
    10321 ggattatgct ttgcaacaag ctgggattat tttttgtcgc tttgtattga ttattttctt
    10381 ttcaactttg ttaaccttaa caaccatgcc tttaagtttg gcatcagctg tcgaagcttt
    10441 attagcacct ttaaagcgtg tgaaagttcc agttcatgaa attggattga tgctgtctat
    10501 gagtttgcgt tttgtcccaa ccttgatgga tgatacgacg cggattatga atgcacagaa
    10561 agctcgtgga gtggattttg gagaaggaag tatcgttcaa aaagtaaagg cgatgattcc
    10621 cattttgatt cctctttttg cgacaagttt aaaacgtgca gattccttgg ctatcgctat
    10681 ggaagcgcgt ggctatcagg gtggaaaagg cagaagtcaa taccgacaat tgaaatggac
    10741 tctaaaggat acgctgacca ttcttgttat tctcgtactt ggttgttgtt tatttttctt
    10801 aaaatcttag tagctttcag gaattgataa aaagttactg taacagtttt tgatataaag
    10861 gtagggatat gaaccgtttt aaaaaatcaa aatatgtcat tattgttttt gtcactgttc
    10921 tgcttgtgtc agctctctta gcgacgactt attcaagtac aattgtgaca aaattaggag
    10981 atggaatctc attggttgat agagttgtac aaaaaccttt tcagtggttt gattctgtca
    11041 aatcagattt ggctcatttg acacgaacat ataatgaaaa tgaaagtttg aagaaacagc
    11101 tttaccaatt agaagttaaa tcaaatgagg tggaaagttt aaagacagaa aatgaacaac
    11161 tgcgccaatt gcttgatatg aagtctaaat tgcaagccac aaagacttta gcagcagatg
    11221 ttattatgcg ttctccggta tcttggaagc aggagttgac cttagatgca ggtagatcaa
    11281 aaggtgcttc tgagaacatg ttagctattg caaatggtgg cttgattggg agtgtttcaa
    11341 aagtagagga gaactctact atagtcaacc ttctgacaaa tacggaaaat gctgataaga
    11401 tttctgttaa aattcaacat ggctctacta caatttatgg aattattatt ggctatgaca
    11461 aggaaaatga cgttcttaaa attagccaat taaatagtaa tagcgatatt agtgcgggag
    11521 ataaggtgac tactggtgga ttaggaaact ttaacgttgc tgatattcct gttggtgaag
    11581 tggttgccac aacgcatagt acagactatt tgacacgaga agtaactgtt aaattgagtg
    11641 cagatactca taatgtagat gtgatagaat tagtggggaa ttcataatga gacagttgaa
    11701 gcgagttgga gtatttttat tgcttccttt ctttgttcta attgacgccc atattagcca
    11761 gcttctgggc tcatttttcc cccatgtaca tttggctagt cattttcttt ttctatttct
    11821 cttatttgag acgatagaag tatcagagta tctctaccta gtctattgtt ttgttatagg
    11881 cttggtttat gatgtttact ttttccatct aatagggatt acaactctct tatttatctt
    11941 attgggagcc ttccttcata aattgaatag tgttattttg ttgaatcgtt ggacaagaat
    12001 gctagctatg attgtgctga cattcctgtt tgaaatgggt agttatcttt tggcttttat
    12061 ggtagggttg acagtagata gcatgtcgat ttttatagtc tatagcttgg taccgacgat
    12121 gattttaaat tttttatgga ttactgtttt tcaatttatt tttgaaaaat attatctata
    12181 agaacgacat ataaatgtaa caaaggcgta atatttatta ggcctttttt tggtatacta
    12241 gtattgtctt taaaagaagg agtatctacg taatatgaag aaaaaaatct tagcgtcact
    12301 tttattaagt acagtaatgg tttctcaagt agctgtttta acaactgcgc atgcagaaac
    12361 gactgatgac aaaattgctg ctcaagataa taaaattagt aacttaacag cacaacaaca
    12421 agaagcccaa aaacaagttg accaaattca ggagcaagta tcagctattc aagctgagca
    12481 gtctaacttg caagctgaaa atgatagatt acaagcagaa tctaagaaac tcgagggtga
    12541 gattacagaa ctttctaaaa acattgtttc tcgtaaccaa tcgttggaaa aacaagctcg
    12601 tagtgctcaa acaaatggag ccgtaactag ctatatcaat accattgtaa actcaaaatc
    12661 aattacagaa gctatttcac gtgttgctgc aatgagtgaa atcgtatctg caaacaacaa
    12721 aatgttagaa caacaaaagg cagataaaaa agctatttct gaaaaacaag tagcaaataa
    12781 tgatgctatc aatactgtaa ttgctaatca acaaaaattg gctgatgatg ctcaagcatt
    12841 gactacgaaa caggcagaac taaaagctgc tgaattaagt cttgctgctg agaaagcgac
    12901 agctgaaggg gaaaaagcaa gtctattaga gcaaaaagca gcagctgagg cagaggctcg
    12961 tgcagctgcg gtagcagaag cagcttataa agaaaaacga gctagccaac aacaatcagt
    13021 acttgcttca gcaaacacta acttaacagc tcaagtgcaa gcagtatctg aatctgcagc
    13081 agcacctgtc cgtgcaaaag ttcgtccaac atacagtaca aacgcttcaa gttatccaat
    13141 tggagaatgt acatggggag taaaaacatt ggcaccttgg gctggagact actggggtaa
    13201 tggagcacag tgggctacaa gtgcagcagc agcaggtttc cgtacaggtt caacacctca
    13261 agttggagca attgcatgtt ggaatgatgg tggatatggt cacgtagcgg ttgttacagc
    13321 tgttgaatca acaacacgta tccaagtatc agaatcaaat tatgcaggta atcgtacaat
    13381 tggaaatcac cgtggatggt tcaatccaac aacaacttct gaaggttttg ttacatatat
    13441 ttatgcagat taatttacag agggactcga atagagccct cttttcaggt tttaccgtga
    13501 caatccctat taaaaattat atcaaaatag cttgaaaata ttggaaaagt atggtagaat
    13561 gaaaattgtc gtgtgaacga taatactcat tcttgatgaa ttgtgaagca gttgcccttg
    13621 ggtcgttttg cgagttgaag tcaagaagag gaaaaaaaca aaaaggagaa atactcatgg
    13681 cagtaatttc aatgaaacaa cttcttgagg ctggtgtaca ctttggtcac caaactcgtc
    13741 gctggaatcc taagatggct aagtacatct ttactgaacg taacggaatc cacgttatcg
    13801 acttgcaaca aactgtaaaa tacgctgacc aagcatacga cttcatgcgt gatgcagcag
    13861 ctaacgatgc agttgtattg ttcgttggta ctaagaaaca agcagctgat gcagttgctg
    13921 aagaagcagt acgttcaggt caatacttca tcaaccaccg ttggttgggt ggaactctta
    13981 caaactgggg aacaatccaa aaacgtatcg ctcgtttgaa agaaattaaa cgtatggaag
    14041 aagctggaac tttcgaagtt cttcctaaga aagaagttgc acttcttaac aaacaacgtg
    14101 cgcgtcttga aaaattcttg ggcggtatcg aagatatgcc tcgtatccca gatgtgatgt
    14161 acgtagttga cccacataaa gagcaaatcg ctgttaaaga agctaaaaaa ttgggaatcc
    14221 cagttgtagc gatggttgac accaatactg atccagatga tatcgatgta atcatcccag
    14281 ctaacgatga cgctatccgt gctgttaaat tgatcacagc taaattggct gacgctatta
    14341 tcgaaggacg tcaaggtgag gatgcagtag cagttgaagc agaatttgca gcttcagaaa
    14401 ctcaagcaga ttcaattgaa gaaatcgttg aagttgtaga aggtgacaac gcttaattta
    14461 tacaaatagt aattacctag gagggcgggg cttagcccgg ctctcctatt ttcaaaaaat
    14521 ataggagaat taaaatggca gaaattacag ctaaacttgt aaaagagttg cgtgaaaaat
    14581 ctggtgccgg tgttatggac gctaaaaaag cgcttgtaga aacagacggt gacatcgaaa
    14641 aagcgattga attgcttcgt gaaaaaggta tggctaaggc agctaagaaa gctgaccgtg
    14701 ttgctgcaga aggtttgact ggtgtttatg ttaacggtaa tgttgcagca gttattgaag
    14761 taaacgctga aactgacttc gttgcaaaaa acgctcaatt cgttgaattg gtaaatacta
    14821 cagctaaagt cattgctgaa ggaaaacctg ctaacaatga agaagctctt gctttgataa
    14881 tgccttcagg tgaaactctt gaagctgcat acgtatctgc aacagcaact atcggagaga
    14941 aaatctcatt ccgtcgcttt gcattgattg aaaaaacaga cgcacaacac tttggagcat
    15001 accaacataa cggtggacgt atcggtgtta tttcagttgt tgaaggtgga gacgaagcac
    15061 ttgctaaaca attgtcaatg cacatcgcag cgatgaaacc aacagttctt tcttacaaag
    15121 aattggatga gcaattcgtt aaagatgagt tggcacaatt gaatcacgtt atcgaccaag
    15181 acaacgaaag ccgtgcaatg gttaataaac cagctcttcc acacttgaag tatggatcaa
    15241 aagctcaatt aactgatgat gttattgctc aagctgaagc tgacatcaaa gctgaattgg
    15301 ctgcagaagg caaaccagaa aaaatctggg acaaaattat tccaggtaaa atggatcgct
    15361 tcatgcttga taacactaaa gttgaccaag cttacacact tcttgcacaa gtttacatca
    15421 tggatgacag caagacagtt gaagcatacc ttgaatcagt taacgcttcg gtagttgagt
    15481 ttgctcgctt tgaagttggt gaaggtatcg agaaagctgc aaacgacttc gaagctgaag
    15541 ttgcagctac aatggcagca gccttgaata actaataata gaaaggaagc aaattgcttc
    15601 cttttttgta tagggcttag atttttcctg aaattgtttt ttccccagtc tcactcgatt
    15661 agaataatta aaacaaaaag ccctataata agggctaagt gcatttagga gactatactt
    15721 caaattcat
//
LOCUS       DAVKWZ010000047        15104 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000047 DAVKWZ010000000
VERSION     DAVKWZ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15104
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.047"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            83..880
                     /locus_tag="VW854_001576"
     CDS             83..880
                     /locus_tag="VW854_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEV0262790.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLIHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            1028..1150
                     /locus_tag="VW854_001577"
     CDS             1028..1150
                     /locus_tag="VW854_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine sulfhydrylase"
                     /protein_id="HEV0262791.1"
                     /translation="MTCDFKSETLQLHAGQVVAPATKSRAVPIYQTTFFVFDDT"
     gene            1172..2212
                     /locus_tag="VW854_001578"
     CDS             1172..2212
                     /locus_tag="VW854_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HEV0262792.1"
                     /translation="MRKPGNIYTRITNPTTATLEGGVEALATASGMTAVTYTILALAH
                     AGDHVVAASTIYGGTFNLLKEPLPRYGITTTFVDVDNLEEVEAAIKDNTKLVLIETLG
                     NSLINIPDLEKLAEIAHKHQIPLVSDNTFAIPYLINVFSHGVDIAIHSATKFIGGHGT
                     TIGGIIVDSGRFDWMASGKFPQFVDEGSSCHNLSYTRDVGAVAFIIAVRVQLLRDTGA
                     ALSPFNAFLLLQRLETSSLRVERHVQNAETIVDFLVNHPKVEKVNYPKLADSPYHALA
                     EKYLPKDVGSIFTFHVKGGEEEARKVIDHLEIFSDLANAADAKSLVVHPATTTHGQLS
                     EKDLEAAGVTPN"
     gene            2383..3657
                     /locus_tag="VW854_001579"
     CDS             2383..3657
                     /locus_tag="VW854_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEV0262793.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMKQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHEALLAKDKEV
                     QLLENQLATLRLEHENQLQKTLSDLEKERDQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKDFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3667..4545
                     /gene="truB"
                     /locus_tag="VW854_001580"
     CDS             3667..4545
                     /gene="truB"
                     /locus_tag="VW854_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEV0262794.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTTTEDASGEVIAETPVLSPLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVIIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALSLEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            4786..5088
                     /locus_tag="VW854_001581"
     CDS             4786..5088
                     /locus_tag="VW854_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV0262795.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQS
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            5196..5834
                     /gene="udk"
                     /locus_tag="VW854_001582"
     CDS             5196..5834
                     /gene="udk"
                     /locus_tag="VW854_001582"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEV0262796.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            5962..7302
                     /locus_tag="VW854_001583"
     CDS             5962..7302
                     /locus_tag="VW854_001583"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEV0262797.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLIIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            7280..7492
                     /locus_tag="VW854_001584"
     CDS             7280..7492
                     /locus_tag="VW854_001584"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEV0262798.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            7489..8364
                     /locus_tag="VW854_001585"
     CDS             7489..8364
                     /locus_tag="VW854_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEV0262799.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVAIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            8357..9172
                     /locus_tag="VW854_001586"
     CDS             8357..9172
                     /locus_tag="VW854_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEV0262800.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            9165..9596
                     /locus_tag="VW854_001587"
     CDS             9165..9596
                     /locus_tag="VW854_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV0262801.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIETFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            9603..11270
                     /gene="recN"
                     /locus_tag="VW854_001588"
     CDS             9603..11270
                     /gene="recN"
                     /locus_tag="VW854_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEV0262802.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEADIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQYGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            11272..12000
                     /locus_tag="VW854_001589"
     CDS             11272..12000
                     /locus_tag="VW854_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEV0262803.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQTQLLFLGDLIDRSEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEAADLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            12079..13902
                     /gene="lepA"
                     /locus_tag="VW854_001590"
     CDS             12079..13902
                     /gene="lepA"
                     /locus_tag="VW854_001590"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEV0262804.1"
                     /translation="MNLEELKKRQGKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
     gene            complement(14061..14141)
                     /locus_tag="VW854_001591"
                     /pseudo
     CDS             complement(14061..14141)
                     /locus_tag="VW854_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            14317..14793
                     /locus_tag="VW854_001592"
     CDS             14317..14793
                     /locus_tag="VW854_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV0262805.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEQLFDQVASLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAEAIA"
     gene            14831..>15104
                     /locus_tag="VW854_001593"
     CDS             14831..>15104
                     /locus_tag="VW854_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV0262806.1"
                     /translation="MIKILAACGAGVNSSYQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGSILFRIPAMSAPVFDNI"
BASE COUNT         4514 a         2741 c         3545 g         4304 t
ORIGIN      
        1 acttttatga aatacagaaa acgcttccta aaacctaaag tttgtgatat aattatcaag
       61 aaaaaagatt taggagttta ttatggtttc ttcagaattt atctcaaaga ttgaatttgc
      121 ttgcaataag aaagaaagtc tttatagtca aagcaaattt aagtatgcga ttcgttcgat
      181 gttcgcaggt gcatttttaa ccttcagtac tgctgcaggt gcagttgggg ctgacttgat
      241 taataaaatt gcaccaggta gtggacgctt cctctttcca ttcgtttttg cttggggctt
      301 ggcctacatt gtttttttga atgccgagtt ggtcacttca aacatgatgt tcttgactgc
      361 tggtagtttc ttaaaaaaaa tctcttggag aaaaacagct gagattttac tatactgtac
      421 cttgttcaac cttatcggag ccttgatagc agggtggggc tttgctcatt cggcagccta
      481 tgcgaatctg atacacgata gtttcatctc aggtgttgtt gagatgaagt taggccgctc
      541 aaatgaattg gtcttgcttg aggcgatttt ggcaaatatt ttcgtaaata ttgcgattct
      601 gtcatttatt ttggtcaaag atggtggtgc caaactttgg cttgtgttgt cagctattta
      661 catgtttgta ttcttaacaa acgagcacat tgcggcgaac tttgcttctt tcgcgattgt
      721 gaaattcagt gttgctgcgg attcaattgc caacttcggt gttggaaata tgcttcgcca
      781 ctggggtgtg actttcatcg gaaactttat cggaggaggc ctcttgatgg gtcttccata
      841 tgccttcctc aataaaaacg aagatactta tgtagattaa gaaaatgagc acgattgagt
      901 cgtgcttttt tcattttcaa aataaggtaa tagctatttc ttatatcaaa atatagaaaa
      961 ctgatatttg taaactataa ctcaaggtgc tacaatatcc ttaataaaat gatatggagg
     1021 tcaccttatg acttgtgatt ttaaatctga aactctacaa ctacatgctg gtcaagttgt
     1081 ggctccagct actaagtctc gtgcagtgcc gatttatcaa acaacatttt ttgtttttga
     1141 tgacacgtag gaaggtgccg atctgtttgc cttgaggaaa ccagggaaca tttatactcg
     1201 tatcaccaat cctacaacag ctacccttga aggtggtgtt gaagcgctag caacagcatc
     1261 aggtatgact gcagtgactt atacgatttt ggcgcttgcc catgctggtg accatgtagt
     1321 ggctgcttcg actatttacg gtggaacctt caatcttttg aaagaacccc ttcctcgtta
     1381 tggtatcaca acaacctttg tcgatgttga taatttggag gaagtagaag cagctatcaa
     1441 agacaatacc aagcttgtct tgattgaaac cttgggtaac tccttgatta atattccaga
     1501 cctggaaaaa ctggcagaga ttgctcataa acatcaaatc ccacttgtgt cagacaatac
     1561 ttttgcaata ccttatttga ttaacgtctt ctctcatggc gttgacattg ccattcactc
     1621 tgcgactaag tttatcggtg ggcatggtac aactattgga ggaataattg tcgatagtgg
     1681 tcgttttgac tggatggctt cagggaaatt ccctcaattt gttgacgagg gttcaagctg
     1741 ccacaatttg agctatactc gtgatgtggg tgcagtagcc tttattatag ctgttcgagt
     1801 tcaattgctt cgtgatacag gtgcagcctt gtcaccattc aatgctttcc tcttgctaca
     1861 aagacttgaa acctcttcac ttcgtgtgga acgccatgta caaaatgctg agacaattgt
     1921 tgattttctt gtcaaccatc ctaaggtaga aaaggtaaat tatccaaaac ttgcagatag
     1981 tccttatcat gccttggctg agaaatactt gccaaaagat gtcggttcaa tctttacctt
     2041 ccatgtcaaa ggtggcgagg aagaagcacg caaggtcatt gatcatttag aaatcttttc
     2101 tgaccttgca aacgcggcag atgctaaatc gcttgttgtc catccagcaa caaccactca
     2161 cggtcaattg tcagaaaaag acctagaagc agcaggtgtc acaccaaact aaattcgttt
     2221 gtcaatcggt cttgaaaatg tagaagattt gattgaagac ttgcgcttgg ccttggaaaa
     2281 aatttaaagt aaaagaagat aaacagtggg cttcgactca ctgtttttga ttttccctca
     2341 ggcatgatat aatggttaaa gaagtctaga aagaggaacg atatgaacga aatcaaatgt
     2401 cccaactgtg gggaagtctt tacagtaaat gagagtcagt atgccgaact cttgtcccaa
     2461 gtgagaacgg cagagtttga taaggaacta cacgatagga tgaagcagga actggccttg
     2521 gctgagcaaa aggccatgaa tgagcaacag actaaactgg ctcagaagga tcaagaaatt
     2581 gcgcaattac agagtcagat ccaaaacttt gatacagaaa aagaattggc caagaaagag
     2641 gttgaacaga caagccatga ggctctcttg gctaaggaca aggaagtaca gctcttagaa
     2701 aatcagttgg ctaccttgcg tttggagcat gaaaatcaac tgcaaaagac cctttctgac
     2761 ctagaaaaag aacgggatca ggttaaaaac caactacttt tgcaggaaaa ggaaaatgaa
     2821 ttatctttgg cttctgttaa gcaaaactac gaagcccagc tcaaggcagc tagtgaacaa
     2881 gtcgagtttt ataaggattt taaggctcaa caatctacaa aagcgattgg ggaaagccta
     2941 gaacagtatg cagagagtga gtttaacaag gttcgtagtt tcgcctttcc aaatgcttac
     3001 tttgagaagg ataacaaggt ctcttcgcgt gggtctaaag gggactttat cttccgtgag
     3061 tgtgatgaaa atggagttga aatcatttct atcatgtttg agatgaaaaa cgaagcagac
     3121 ggaacagaga agaagcacaa gaatgcagat ttttacaagg aattggacaa ggaccgtcgg
     3181 gagaagaact gtgagtatgc cgttttggtg accatgcttg aggccgataa tgactacttt
     3241 aacacaggga ttgttgacgt cagccacgag tatgaaaaaa tgtatgttgt tcgtcctcaa
     3301 ttctttatcc agttgattgg tctcttacgt aatgcggcgc taaattccct aaaatacaag
     3361 caggagttgg ccttggttcg cgagcaaaat attgacatta cgcattttga ggaagacttg
     3421 gatgccttta agctagcttt tgctaagaac tataattcag cttcgactaa ctttggaaaa
     3481 gctattgatg aaatcgacaa ggccatcaaa cgcatggaag aggttaagaa attcctgact
     3541 acatctgaaa accaactccg tctagctaac aacaaattgg aagatgtctc tgttaaaaaa
     3601 ttgacccgga aaaatccaac aatgaaagcg aagttcgaag cactgaaggg ggagtagaaa
     3661 gcaaaaatga acggtattat caacttaaaa aaagaagcag gaatgacctc gcatgatgcg
     3721 gtttttaaac tgcgtaagat tttgggaacc aagaaaattg gtcatggtgg aaccttggat
     3781 ccggatgtgg tgggtgtttt gccgattgcg gttggcaagg cgacacgcat ggtcgagttt
     3841 atgcaggacg agggtaagat ctatgagggg gaaattactc tgggttattc cacgacgact
     3901 gaggatgcta gtggggaagt gatcgcagaa acccctgttt tgtctccctt ggatgaaaag
     3961 cttgttgatg aagcgattgc tagcttgact gggcctatta cccagattcc ccctatgtat
     4021 tcggcagtta aggttaatgg tcgcaagctc tatgagtatg cgcgtgctgg tcaggaagtg
     4081 gagcgtccag aacgtcaggt gatcatttat caatttgagc gaacaagtcc gatttcttat
     4141 gatggccaac ttgcccgatt cacttttcgt gtaaaatgca gtaaagggac ttacatccgt
     4201 actttgtcag ttgatttggg tgaaaagctt ggttatgcgg ctcatatgtc ccatttgact
     4261 cgtactagtg ctgctggctt acaattagaa gacgctcttt ccttggagga aattgctgaa
     4321 aaagtagagg ctgggcaatt agattttctc catcctttag agattgggac aggtgacctt
     4381 gtcaaagttt tcctaactcc agaagaggct acagaagttc gctttggtcg ttttattgag
     4441 ctagaccaaa cggacaaaga actggctgcc tttgaagatg ataaattgtt agccattcta
     4501 gaaaaacggg gcaatctcta taagccaagg aaggttttta gctagatcgt ttaggaataa
     4561 aaatcgggtg atagataaca attgcttgat aaaaccccat actaatagta gaatggtttt
     4621 gggaattata atattccaat tgttgcgagt tgtaggtact caaataatct atatagaaat
     4681 ttagaggtgt gaaatgaagc aatttaaaat tctttcagat aaatatttag agtccattac
     4741 aggttctggt gggaacttag gcccaggatt tggtgtgata attccatgat gcgaaatgag
     4801 tttcgagaaa gggtggagca acttcttcaa caaaaagaaa taaatgaaaa tagtgagttg
     4861 agtcacctgt ttcgtcttgc tatacaaaat ttagacagaa atgaaaaata ccaatcggtc
     4921 atggccaatt tgagtcaagg gttgtcactt tacctcatga cgcatcatta ccaggcacct
     4981 aagtctgtca ttgattttgg tttatggatt gccaaagctc ctagccagga aagagggaga
     5041 ctggctttct tgcagatgct tgctcaaacc ctacagggct ttcgttaaaa ctgtcgaaca
     5101 tcagagggaa gactctggtg ttttttgctg atgagactat aaattttgta aaaaatgtga
     5161 tagaatagac gacggataaa ataacggagg atagcatgca aaatagacca atcattattg
     5221 gagtgacagg tggttctggt ggtggtaaaa ccagtgtttc aagagccatt ttatcgcatt
     5281 ttcctgatga aaagatttcc atgattgagc atgattcata ctacaaggat cagtctcatt
     5341 tgacctttga agagcgtgtc aaaaccaact atgaccatcc ttttgccttt gatacagact
     5401 tgatgatcga gcagattaag gaattgttgg cagggcgtcc ggtggacatc ccgacctacg
     5461 actatacaga gcatacacgg agtagcaaga cctatcgtca ggagcctcaa gatgtcttta
     5521 tcgttgaggg cattttggtc ttggaggaca agcgtctgcg cgatttgatg gatatcaaga
     5581 tttttgtgga tacggatgac gatgtgcgta ttattcgtcg tatcaagcgt gatatggagg
     5641 aacgtggccg tagccttgat agcgttatta accagtactt aggtgtggtc aaaccaatgt
     5701 accaccagtt tatcgagtca actaagcgtt atgctgatat cgtcattcct gaaggggtta
     5761 gcaataccgt ggctatcgac ctgttgacga ccaagattgc aaagattttg gaagaagctc
     5821 gaaacagcaa ataatcacat gtggaggcct tgcctccttt ttctattttt ccctcataat
     5881 ggtacataaa tggagatttt taggcatatt tttggtataa taatacccat aaaagagcaa
     5941 gtaaatgaat agtaggtgga gatggaaaag tatttatcgg taacaacttt gaccaaatat
     6001 ctgaaaatga aattcgataa agacccatac ttggaacggg tctatttaac tggtcaagtt
     6061 tccaactttc gtaaacgacc tactcaccaa tatttctccc taaaggatga ccatgcagtt
     6121 attcaagcga ccatctggtc tgggatttat cagaaattag ggtttgacct ggaagaagga
     6181 atgaagatca atgtgattgg gcgtgtacag gtctatgaac caagtggtag ctactccatc
     6241 atcattgaaa aagctgagcc tgatggggtt ggggcgcttg cgattcagtt tgaacaactt
     6301 aagaaaaaat tgacagaaga aggcctgttt caagaacgct tcaagcaagc tctaccccaa
     6361 ttttctaaga gaattggggt ggtaaccagc cgtagtggag ccgttattcg agatattatc
     6421 acaaccgtca gcaggcgatt cccaggtgtt gacatccttc tttatccgac caaggttcaa
     6481 ggtgaagggg ctgcggagga aattgctaga aatattgcgc gtgctaatca acgggacgat
     6541 ttggatttga tcattattgg tcgtggaggt ggttctattg aggatctctg ggcctttaac
     6601 gaagaaattg tggtacgagc tatttttgaa tctcgtttgc cagttatctc tagtgtgggg
     6661 catgagacgg atgtgacctt ggcagatttt gtggcagatc gacgcgctgc aacgccaaca
     6721 gcggcggctg aactggcaac acctgtgacc aagttggatg tattagctca tttgcaaaat
     6781 caggaaaaac ggatggcaac ggcagtccga aatgttctat ctaagaaaca agaggctttg
     6841 aaaaaatgca gtcagtctgt tatctttaga caacctgagc gcttgtatga cggttatttg
     6901 caacgcttgg accaactgca actgcgtttg aaacaaagtt tgcgaactcg gatttctgat
     6961 aacaaacaat tagttcaagc aagaactcat caattagtac aattatcacc tgttaccaaa
     7021 atccaacgct atcaagaccg tttaggacag ttggacaagc tcttaggtag ccaaatggcg
     7081 ttagtttatg acgccaaggt tgctgaggcc aagcgacttt cggaagcttt gctcatgttg
     7141 gatactagcc gaatcgtggc gcgtggttat gctattgtca aaaaagaaga gtccgttgta
     7201 gattcggttg agagtttgaa gaaaaaagac caagtaacgc ttttgatgcg agatggtcaa
     7261 gtagaattag aggttaaaga tgtcaaaaca aaagaaattt gaggaaaatc tagcagaact
     7321 ggaaaccatt gtccaaagtt tggaaaatgg tgaaattgct ctggaagatg cgattactgc
     7381 ctttcaaaag ggcatggtct tgtcaaaaga gctccaagct acgctggaca aggctgaaaa
     7441 gaccttggtc aaggtcatgc aagaagacgg aacagaaagt gattttgaat gaaaaagcaa
     7501 gaaaaattag ctcttgtcga gtcggctttg gaagattttt atggagacca gcagtttgcc
     7561 tctagtttac gggagtctgt tctctattct attcatgctg gtggcaagcg tattcggcct
     7621 tttctcttgt tagaagttct ggaagccttg caggttgcca tcaaacctgc tcacgcgcag
     7681 gtagctactg ccttggagat gattcataca gggagcttga ttcacgatga ccttcctgct
     7741 atggatgatg acgattatcg aagagggcgg ttaaccaatc acaagaaatt cggtgaagct
     7801 atggccattt tggctggaga tgccttattc ttagacccat atgccttgat tgcgcaggca
     7861 gatttgccaa gtcagatcaa ggtggacttg attgccaact tatcccttgc ttcaggtagt
     7921 ctgggtatgg tggcagggca agttttggat atggagggcg aacaccagca cttgtctctg
     7981 gaagaacttc agactattca tgccaataag actgggaagt tactagccta tcccttccaa
     8041 gcggcagcta ttatagctga attgtcacct gaaatgcagg tgaagctgaa aactgtgggt
     8101 gaattgattg gacttgcttt tcaagtcaga gatgatgtac tggatgtgac agctagtttt
     8161 gaggaaatcg gcaagacacc tcaaaaggat ctgcaggcag aaaaatcaac ctatcctgcc
     8221 ttgttgggct tggaagagtc cattgccttt tgtaaccaaa ccctggatca agctaatgaa
     8281 aaattagaag aaattgccca gcagcttccc tttgaaacag aatcgattgt aagtgtagta
     8341 gaaagtttga gaatcaatgg ctaaggaaag agtggatgta ctagcttata aacaggggtt
     8401 gtttgaaacg agagagcagg ccaagcgagg tgtgatggct ggcctagtcg tagcagtcct
     8461 taatggagaa cggtttgaca agccaggaga gaaaattcca gatgacaccg aattaaaact
     8521 caagggggag aaactcaagt atgtcagccg tggtggtttg aaactggaaa aggccttgca
     8581 ggtctttgat ttgtcggtgg atggcgcgac tacgattgat atcggggcct ctactggagg
     8641 ttttaccgat gtcatgctac agaatagtgc caagttggtc tttgcagtcg atgttggtac
     8701 caatcagttg gcttggaaat tacgccaaga cccacgagtt gtcagcatgg agcagttcaa
     8761 tttccgctat gctgaaaaga ctgatttcga gcaggagccg agctttgcca gtattgatgt
     8821 gagtttcatt tcccttagtc tgattttgcc agccttgcac cgtgtcttgg ctgatcaagg
     8881 tcaggtggta gcacttgtca aacctcagtt tgaggcagga cgtgagcaga ttgggaaaaa
     8941 tggaattatt cgagatgcta agattcatca gaatgtcctt gaatctgtaa cagctatggc
     9001 agtagaggca ggtttttcag tccttggctt ggacttttct cccatccaag gtggacatgg
     9061 aaatattgaa tttttagcgt atttgaaaaa agaaaagtca gcaagcaatc agattcttgc
     9121 tgagattaaa gaagcagtag agagggcgca tagtcaattt aaaaatgaat aaaaaagaga
     9181 gacttgaaaa aattagaaga ttggtgacag attatcaaat cggcacgcaa gaagaaattg
     9241 tagaacattt gaaagaagca ggtatcactg ccactcaggc gacggtatcc cgagatatca
     9301 aagagttagg tattgtcaaa attcctttga gagacaacac ctatgtctac gaattgccaa
     9361 aatcaatcgt aaaaagtctg caactggctg aagacaatat cgaatcggct gaattgatgg
     9421 ataagatgat caatctccaa gttattccag gaaatacggc ttttgtaaaa gctcagttaa
     9481 tcgagacttt tgcagacaag atttttagct gtttgactga tgatagctcg attttagtca
     9541 ttgccagaag tggaagtcta gcagaggaaa tctttgaaca agtaaaaaat tggtaggtct
     9601 atatgttact tgaaatttcg ataaaaaact ttgccattat tgaggctatt tccctcaatt
     9661 ttgaaaaggg gatgactgtc ctgactggtg aaacgggtgc agggaagtcg attatcattg
     9721 atgccatgaa tatgatgttg ggagctcgtg cgacgacaga tgttattcgt catggtgcac
     9781 caaaggcaga gattgagggg cttttctcag ttgagaatag tcgtctttta caggaaattt
     9841 ttgatgagca aggtttagaa ttaggtgatg aaattatcat ccgtcgggaa atcttgcaaa
     9901 atggtcgtag tatcagccgt gtaaatggtc agatggttaa tctgtctgtt ttgcgagcta
     9961 ttgggcaaca tcttgtagat attcatggtc agcatgacca tgaggagtta atgcgtcccc
    10021 aactgcatat ccagatgttg gatgaatttg gtgatgccgc tttttgggac ttgaaagaaa
    10081 cctatcaaac gagttttgat gcctatcgga aaatgcgtaa gcaggttctg gaagtcaaga
    10141 aaaaccaaca ggaacacaag gcacgtattg aaatgttgga atttcaaatg gcagagattg
    10201 aggcagcaaa cttgcaggct ggagaagact tggctctcaa tcaagagcga gataaactcc
    10261 tcaaccacaa aaatattgcg gatacactga ccaatgccta cagtatgttg gacaatgaag
    10321 atttttcaag tctggccaat gttcgttcag ctatgaatga catggaaagt gtcgaagagt
    10381 atgatcctga ataccgtgaa atttcaagct ctctgtctga gacctactat gttttagaag
    10441 acattagcaa acgtttggaa gctatcattg aggaccttga ttttgatggc aatcgcctga
    10501 tgcaggttga gaatcgtttg gacctccttc atactattac tcgtaagtat ggtgggactg
    10561 ttgatgatgt tttgctttat tttgccaaga ttacggaaga atacaatctc ttgacaggca
    10621 ataatctttc gtctgaggac atggaagcag agcttaagaa gttggaagtc aatcttgtca
    10681 atttggcagg tcaacttgct tctgctcgtc ataatttggc tcagcaactc gaagctgaca
    10741 ttaaacaaga actgcaagat ctttatatgg aaaaagccca gtttcaggtt cgttttagta
    10801 agggaaaatt cagtcgtgag ggaaatgaaa tggttgagtt ttatatttca accaaccctg
    10861 gagaagactt taaacccttg gttaaggttg cttctggagg ggaattatct cgtctcatgt
    10921 tagccattaa gtctgccttt tcacgtaaag aaggcaagac tagcattgtc tttgatgagg
    10981 tggatacggg agtttcaggt cgtgttgctc aagctattgc tcagaaaatt cataaaattg
    11041 gtcagtatgg tcaggttttg gctatctccc atttgccaca agtaattgcg attgcagatt
    11101 atcaattttt tattgagaag attagtaatg accattcaac ggtttcgact gttcgtctct
    11161 tgacagtcga agagcgagtg gaggaagttg ccaagatgtt ggcaggtgat gatgtgacag
    11221 aagcagccct gacgcaagcc agagaattgt tgagaaacag ggagaaataa gatgacagat
    11281 tattatgtaa ttggagatgt tcacggaaaa gctgggatgc tggaagacct tcttaaaacc
    11341 tgggatggtc agacccagtt gctctttcta ggtgatttga ttgacagaag tgaggatagt
    11401 caccgtgttc ttgaaatggt caaggacttg gtggacaatc aaggggctat ctgtttgtct
    11461 ggaaaccacg agtatatgtt tttgacttgg ctagatgacc cagaagaaag ttatgaccat
    11521 tatcgtcgca atggtggaga tacaaccatt aactctatcc taggtcgtcc cttggatgca
    11581 ccagttgatg gagtagaaga tgccaagcgg gttgccgctg aagcggcaga cttggtcgaa
    11641 ttcattcgtc aaatgccatt tgtagtagag acagacaagt atatctttgt tcacgcaggt
    11701 attgatttga ccttggacga ctggcatgaa acaacagatt ataaaaaagt atggcttaga
    11761 aaaccattcc acggagctga aaatcatact ggaaaaacca ttgtcttcgg tcatacaccg
    11821 gtctatggtt tgttacagca agaacgcggt acatctgagc tttggactac agaagatggc
    11881 aagattggaa tggatggagg agctgtttat ggtggtgtcc ttcacgggat tgtctttaca
    11941 gaccaaggaa tgacagaaca ccactttatc gaaaatgacg gctttgtcgc tgaagattag
    12001 tactcctagc agggtatggt cttgtcaaaa tatcaaaaac aatttataat aaatagatac
    12061 cctgaaagga agagaatcat gaacttagaa gaattgaaaa aacgacaggg gaagatccgt
    12121 aacttctcta ttatcgccca tattgaccac gggaaatcaa ctctagcaga ccgcattttg
    12181 gaaaaaacag agacggtttc aagtcgtgaa atgcaggccc agcttttgga tagcatggat
    12241 ctagagcggg aacgtggaat tactattaag ttgaatgcca tcgagttgaa ttacactgca
    12301 aaagatgggg aaacttatat tttccacttg attgacacac cagggcacgt tgactttacc
    12361 tatgaagttt cacgttcgct agctgcctgt gagggtgcta ttttggtggt cgatgcggct
    12421 caaggaattg aggctcaaac tcttgccaac gtttatctgg ctttggataa tgatttggaa
    12481 atcatgccta tcattaataa aattgacctg ccggctgcag atccggagcg cgtgcgtaca
    12541 gagattgaag atgtgattgg tttggatgcc agtgaagcag ttttggcttc tgccaaggct
    12601 ggtattggga ttgaagaaat cctcgagcaa attgtagaaa aagtaccagc accaacgggt
    12661 gatgtgacgg cgccacttaa ggccttgatt ttcgactctg tttacgatgc ttaccgtggg
    12721 gttatcctcc aagtgcgtgt catggacgga gtggtcaaac ctggtgataa gattcagctc
    12781 atgagcaata gtaagacctt tgatgtggcc gaagtcggta tttttacacc aaaagcggtt
    12841 ggtcgtgatt tccttgcgac tggtgatgtt ggttacattg cggcgtctat caagacggtt
    12901 caggatactc gtgtgggtga taccgttacc ttggcaacca atcctgcggc agaaccatta
    12961 catggttata agcagatgaa tcctatggtc tttgcgggtc tctacccaat cgaatcaaac
    13021 aagtacaatg acctgcgtga agcgcttgaa aaattgcaac tgaatgatgc tagtcttcag
    13081 tttgaaccag aaacatctca ggcacttgga tttggtttcc gttgtggatt tcttggactt
    13141 ctccatatgg atgttattca ggaacgttta gagcgtgagt tcaatattga cctcatcatg
    13201 acagctccgt ctgttattta caaagttaat ttgaccgacg gtgagtctat ggatgtgtct
    13261 aacccatctg agtttccaga cccaactaag attgcgacca ttgaagagcc ttatgtcaag
    13321 gcgcaaatca tggtaccaca ggagttcgtc ggagcagtaa tggagctagc tcagcgtaag
    13381 cgtggggact ttgtgactat ggactatatt gatgataacc gtgtcaatgt tatctatcaa
    13441 attcctcttg ctgaaattgt ctttgacttc tttgataaac ttaagtcttc gacacgtggt
    13501 tatgcaagct ttgactacga attgtcagaa tatcgcccat ctaagctggt gaaaatggat
    13561 atccttctca atggagataa ggtggatgcc ctcagcttta tcgttcacaa ggactttgcc
    13621 tacgaacgtg ggaaactcat cgttgataaa ctcaagaaaa tcatccctcg ccaacaattt
    13681 gaagttccaa tccaagcggc tattggacac aagattgtcg ctcgtactga tatcaaggcc
    13741 cttcgtaaga acgtacttgc taaatgttat ggtggtgacg tttctcgtaa gcgtaaactc
    13801 cttgaaaaac aaaaagctgg taagaaacgc atgaaatcca tcggatcagt tgaagttccg
    13861 caagaagcct tcctcagcgt cttgagtatg gatgaagaat aagttatcag aaatgccttt
    13921 ccttgttcac aggaaattta tataaaaatc aatagtaaag caggcttaga tggtctgctt
    13981 ttttagtttt tatactcttc gaaaatctct tcaaaccacg tcagcgtcgc cttaccgtat
    14041 gtatggttac tgacttcgtc agtttcatct acaacctcaa aactgtgttt tgaacaacct
    14101 gcggctagct tcctagtttg ctctttgatt ttcattgagt attagaacag aaaaaatgct
    14161 tggagtattt gtttgtgtgt ttacttttat ataacaaact ataaacacaa caaaaatata
    14221 aaaaaagaga caaaaaaaga acagaaagta attaacaacg atttcatata gtgttatact
    14281 tatagcataa agttaatgga aagaagggaa aaccttatgg tattagatta tttctttgac
    14341 aaaaaccttg tgttttgctt agaagcggat aatcaagaac aactctttga tcaggtagca
    14401 agcttgttgg aagaacgaga aattgtcact ccaacttatc gtgaagcctt gatcacgcgt
    14461 gaaaagtcat ttccaactgg tttagatatg gaatttctag gaaaggactt gccaaatgta
    14521 gcgattcctc acacagatat tgttcataat ctagctgaaa aagtggtggt tgttcgatta
    14581 gaaaaaccag taacttttca caatatgata gctcctgata aggaagtaga agtatcccta
    14641 ctcttcttta tcattaacaa ttcaagttca agtcaaacaa atattctggc tcagttgatg
    14701 gactttttca caggaaatgg acatcttgaa gatttatcaa aaatttccga accagaaaaa
    14761 ctttatgctt acattgctga agcaatcgct taatcttgtc tattaaaaaa taaaatcgga
    14821 ggaaatccaa atgattaaaa ttcttgctgc ctgcggtgca ggtgttaact caagttacca
    14881 aattaaaagt gctctagaag aagaactttc aaaccgtggt tatgatgttc actgtgatgc
    14941 agtcatggtg aaagatgtaa acgaagacct tatgaaaggt tacgatatct ttacaccaat
    15001 cgctgcaaca gatcttggtt ttgaaccagg tatcccagtt atcgaagctg ggtcaatctt
    15061 attccgtata ccagcaatga gcgctccagt atttgacaat atta
//
LOCUS       DAVKWZ010000048        15099 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000048 DAVKWZ010000000
VERSION     DAVKWZ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15099
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.048"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            160..720
                     /locus_tag="VW854_001594"
     CDS             160..720
                     /locus_tag="VW854_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV0262807.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     VFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     gene            721..2106
                     /locus_tag="VW854_001595"
     CDS             721..2106
                     /locus_tag="VW854_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0262808.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHVQAFTLLVGSSGSGKSSLFQMI
                     AQVSSLPYSGQVLIDGSEVSQLSIVERVQKVGILFQNPNHQFTMENLFEELIFTLENI
                     GYHLQEIDSKIAEVVQRCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMNRVTGELGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGSITWKGSVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVMLLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDSLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            2108..2758
                     /locus_tag="VW854_001596"
     CDS             2108..2758
                     /locus_tag="VW854_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEV0262809.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLTQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            2769..3461
                     /gene="tenA"
                     /locus_tag="VW854_001597"
     CDS             2769..3461
                     /gene="tenA"
                     /locus_tag="VW854_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEV0262810.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKEMEISDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            3466..3990
                     /gene="thiW"
                     /locus_tag="VW854_001598"
     CDS             3466..3990
                     /gene="thiW"
                     /locus_tag="VW854_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEV0262811.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFAERIE"
     gene            3990..4793
                     /locus_tag="VW854_001599"
     CDS             3990..4793
                     /locus_tag="VW854_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV0262812.1"
                     /translation="MQALTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRMAALYANQSSLPMVVDAVGITTSSIRKS
                     LVKDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLIVSENQVAVLENGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            4786..5418
                     /locus_tag="VW854_001600"
     CDS             4786..5418
                     /locus_tag="VW854_001600"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV0262813.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVRLIIDDRLDVCLAVDAAGLHIGDDELPVSVARKVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPV
                     VAIGGLTSENIDQLIGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(5627..6418)
                     /gene="thiD"
                     /locus_tag="VW854_001601"
     CDS             complement(5627..6418)
                     /gene="thiD"
                     /locus_tag="VW854_001601"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV0262814.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVYKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     regulatory      complement(6502..6600)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6784..7179
                     /locus_tag="VW854_001602"
     CDS             6784..7179
                     /locus_tag="VW854_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEV0262815.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     gene            7190..7561
                     /locus_tag="VW854_001603"
     CDS             7190..7561
                     /locus_tag="VW854_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEV0262816.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEIMPE
                     QAGEFGFACGMNMMHGKMIVE"
     gene            7571..9814
                     /locus_tag="VW854_001604"
     CDS             7571..9814
                     /locus_tag="VW854_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV0262817.1"
                     /translation="MTEIVKASLENGIQKIRIRAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLAGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDIIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQALHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            10021..11796
                     /gene="spxB"
                     /locus_tag="VW854_001605"
     CDS             10021..11796
                     /gene="spxB"
                     /locus_tag="VW854_001605"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEV0262818.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAISKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            11907..12254
                     /locus_tag="VW854_001606"
     CDS             11907..12254
                     /locus_tag="VW854_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV0262819.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(12371..12612)
                     /locus_tag="VW854_001607"
                     /pseudo
     CDS             complement(12371..12612)
                     /locus_tag="VW854_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            12783..13064
                     /locus_tag="VW854_001608"
     CDS             12783..13064
                     /locus_tag="VW854_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEV0262820.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYKDGVDLLGYTTWG
                     CIDSVSAGTGEMNKRYGFIYVDRDNVGNGTLKRSKKKSFYWYMSFIAMV"
     gene            13196..14140
                     /gene="manA"
                     /locus_tag="VW854_001609"
     CDS             13196..14140
                     /gene="manA"
                     /locus_tag="VW854_001609"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEV0262821.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDTLLTKVPVKAG
                     DFFYVPSGTMHAIGTGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTVKADNLRSTLLVSNDFFAVYKWEITGKVDFEKTADYSLFSILAGQGQL
                     TVDGKNYPIQKGSHFILPSDVESWTLEGQDLELIVSHP"
     gene            complement(14180..>15099)
                     /locus_tag="VW854_001610"
     CDS             complement(14180..>15099)
                     /locus_tag="VW854_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEV0262822.1"
                     /translation="IISNPAIALGAQAAFILLNIFIVSRGVQKGIERASKVMMPLLFI
                     IFVVIIGRSLSLSNAMEGVLYFLKPDFSKLTSAGLLYALRQSFFALSLGVTAMLTYAS
                     YLDKKTNLVQSRISIVAMNISVSIMAGLAIFQARSPFNIQSEGGPSLVFIVLPQLFDK
                     MPFGTIFYVLFLFATVTSSVVMLEINVDNITNQDNSKRAKWSVILGILTFVFGIPSAL
                     SYGVMADVHIFGKTFFDAMDFLVSNLLMPFGALCLSLFTGYIFKKALAMEELHLDERA
                     WKQGLFQVWLFLLRFVIPIIIVVFIAQFM"
BASE COUNT         4376 a         2862 c         3384 g         4477 t
ORIGIN      
        1 gattgaaata agatgcgaac aaatcaatta gaaaattcaa attaatttct agaaatattt
       61 tagcattctt gtgtactgtt ctaaattcag tctgctatac aaagtattta ggtcggtctt
      121 cttgagtgcc ccacttttct ttaaaaaaag gagtttttta tgttgaaaaa atggcagtta
      181 aaagatgtta tcttgcttgc tttcttgtct atcttttttg gtggggtttt cgttggttca
      241 ggatatgtgt ataatattct cagtctactc ttaacacctc ttggtttgca ggtctttgcc
      301 aatgaaatcc tcttcggtct ctggtgtatg gctgcgccca ttgctgccat ctttgttccg
      361 agagtcggaa gtgcaacgat tggagaagtg ctagctgcgc ttgctgaagt cctttatggt
      421 agccaatttg gtctaggagc tcttttgtct ggcttggttc aaggtttggg aagtgaattt
      481 ggttttatcg taactaagaa tcgctatgaa agttggctct ctctaactgc taatagtatt
      541 gggattacgc ttgttagctt tgtctatgaa tacattaagt taggttacta cgccttttcc
      601 cttccgtttg tcctttcctt acttgtggta cgttttattt ctgtttattt cttctgtacc
      661 atcttggttc gtgccattgt caaactctat catcagtttg caactggagg aaaagcatag
      721 atgggtctgg aactacgagc gattcagtcc ccaatcttct ctgagccgtt tgattttact
      781 tttcatgtgc aagcctttac cttgttagtt gggagtagtg ggtctggaaa atcgagcctc
      841 tttcaaatga ttgcccaagt tagttctctt ccctatagcg gtcaagtcct gatagatggg
      901 agtgaggtca gtcagctttc tatcgtcgaa cgtgtccaga aggttggcat tctctttcaa
      961 aatcccaatc atcaatttac catggagaac ttgtttgagg agctgatttt caccttggag
     1021 aatatcggct atcaccttca ggaaattgat tctaaaatag cagaggttgt ccagcgatgt
     1081 cgttgcgagg caatcttaca ccgtcccatt catcacctat caggtgggga aaagcaaaaa
     1141 gcagcgctgg ctgtcctctt tgccatgaat cctagggtat atctcttgga tgagcccttc
     1201 gcttccattg accgcaagag cagaatcgag atattggaga ttctaaaaga gttggctctt
     1261 gatgggaaga cagttatttt gtgcgaccat gatttatctg actataaagc ctatatcgac
     1321 catatggttg agctaagaga cggaaaacta agggaagtgt ttcaaatccc ttcctatgag
     1381 atgacacagg ttgcttcaaa ggaagttgct tctagcccgg aactattcca tatgaaccgt
     1441 gtgactggtg agcttggtaa tcgccccctc ttttcaattg ctgatttcac attctatcaa
     1501 gggatttcct gtatcctggg tgacaatggt gtcgggaaat caaccctctt tcggtctatt
     1561 cttcaatttc aaaagtataa ggggagcatt acttggaagg gttcggtcct gaaaaagaaa
     1621 aagagtttgt atcgtgatct gactggtgtt gttcaggaag ctgagaagca gtttatccga
     1681 gtcagtctgc gagaggagct tcaattagat ggacctgatt ctgaaagaaa tcagcggatt
     1741 tttcaagctt tacgatattt tgatttggag caggcagtcg ataagagtcc ctatcaatta
     1801 agtggtggtc agcaaaaaat tcttcagctc ttgaccatct tgaccagtaa ggcttccgtg
     1861 atgttgctag atgaaccttt tgcaggtttg gatgatagag cctgccatta tttttgcaag
     1921 tggattgtgg aggagaggaa tcaaggaaga agttttctgc tcattagtca tcgtttagac
     1981 tctttgattt ctgtggttga ttattggatt gagatgacta gtcagggtct tcggcatgtg
     2041 aaagaagtga ccattaccaa accacttaca tctcagagta gcaataccca aggggaggtg
     2101 agatagtatg gtcaaagtag caacccagac accgattatc agtctcttct tgctgatttt
     2161 atccttggaa acatctttca ttccttcgat tgctctgaat ctttcggtag tcgcattttg
     2221 tattctcttt atgctctatt accgtcgatt taaaatgtta gcttggatga tcatacttgc
     2281 cattttacca tcttttgcca actactgggc agttcagtta cacggagatg cttcacaggc
     2341 agtcatgctt ggaacgaggg cctttgtgac agtttgtatc ggccttgtct ttgtttcctc
     2401 tatttcacta aaagagcttc tcttgtactt gactcaaaag gggctatcac gctcttggtc
     2461 ctatgccttg attgtggtat tcaattcctt tcctctcatt cagcaagaaa tcaagtccct
     2521 caaagaagct tgcctattac gtggtcaaga actgcatttt tggtcgccct tgatttacag
     2581 taaggttctg atgacagtct ttaggtggcg ccatctttac ctgagagctc tgtctgctca
     2641 tggatatgac gaacatgcac agttgaagaa tagctatcgg actttttata ttcctaaaaa
     2701 aacaaaatta atctacctgc ttttcttttt attgcttcaa accagtctat ttttataaag
     2761 gagttattat ggaatttaca gatattgcga tggaattatc caagaaagct tggcaggctt
     2821 cctttcatca tccctttatt ttacaattac aagaggggaa tttagaacct gccattttcc
     2881 gctattacct gattcaggat gcctactatc tgaaggcctt ctcagaaatc tatcatcttt
     2941 tggctgataa gacttcaaac caagagatga aaagactctt gaaacaaaat gctcagggtt
     3001 tagtggaggg tgagttattt attcgccaac aatttttcaa ggaaatggaa atcagcgacc
     3061 aggaaatgga gcaacatcca attgctccaa cctgttatca ttacatttct catatttata
     3121 ggcaatttgc agaaccgaac ttagccatcg cttttgcaag cttgcttcct tgtccttggt
     3181 tataccatga tataggcaaa tcacttaatc ttaaaccatc accaaatcct ctctatcaac
     3241 aatggattga aacttatatt acggatgagt tagagcaaca gatcagagag gagggagcac
     3301 tggtcaatca gctctatcga gaaagtgatg agacagataa gcaaaaaatg ctagatgcct
     3361 tccacatcag tgttcatatg gaagccaagt tttgggagat ggcttaccaa caccagacat
     3421 ggaagagcga tttacagtct ttagaaaaag gagaagaata gaaacatgag aaagcaccaa
     3481 ctacaagttc acaaattgac cattttatct atgatgattg cccttgatgt agtccttaca
     3541 cctatctttc ggattgaggg aatggcaccg atgtccagtg tagtcaatat tctagcggga
     3601 atcatgatgg gacctgttta tgccttggct atggctacag tgacagcctt tatccgtatg
     3661 acgactcaag ggattccgcc tttagctctc acaggagcga cttttggagc ccttctagca
     3721 ggtctctttt ataagtacgg tcgaaaattt tacttttctg ccttgggaga aattgtggga
     3781 acaggtatta ttggttctat tgtttcctat cctgttatgg tgctctttac aggatcggct
     3841 gctaagctta gttggtttat ctacacgcct cgatttttcg gagcaacctt gattggtaca
     3901 gcgatttcct ttattgcatt tagattttta atcaagcagg aattctttaa aaaagtgcaa
     3961 ggatatttct ttgctgaaag gatagaatga tgcaggcatt gacaaatcct tttcctatag
     4021 gctctagttc cctcattcac tgtatgacca atgagatttc ttgtgagatg ctggcaaatg
     4081 ggattttggc tctgggatgc aaacctgtca tggcagatga tccccgtgaa gttcttgatt
     4141 ttactaagca aagccaggct ctcttcatca atttggggca tttgtcagct gagaaggaaa
     4201 aagcaatccg catggcagct ttgtatgcaa accaatcttc tctcccgatg gtagtagatg
     4261 cggttggcat aacgacttca tccattcgta agagcttagt taaagacctt ttagactata
     4321 gacctacggt ccttaaagga aacatgtcag aaattcgaag tcttgttgga ttaaagcatc
     4381 acggcgttgg ggtcgatgcg agtgctaaag atcaagaaac ggaggatttg cttcaagtct
     4441 tgaaagactg gtgtcagacc tatcctggta tgtcattctt agtcacaggt cccaaggacc
     4501 ttattgtttc ggagaatcaa gttgctgtac ttgagaatgg ctgtactgaa ttagactgga
     4561 taacagggac aggagacttg gttggagcct taacagctgt ttttctcagc caaggaaaga
     4621 ctggttttga agcttcttgc ttagcagtct cttatctcaa tatcgctgct gagaaaatag
     4681 ttgttcaagg aatgggattg gaagaatttc gttaccaagt actcaatcag ctttcgctcc
     4741 taagaagaga tgaaaattgg ctagatacca tcaaaggaga ggtttatgaa tagagaagcg
     4801 cttagactat atctggtaac caatcgctat caagattccg tggaaagctt tcttgcaaaa
     4861 gttgagacgg cctgccgttc aggggttacc atagtccaat tgcgagaaaa aaatctcaca
     4921 accaatcaat attatcaact ggcaaaacaa gtcaaggaaa taacagatgc ctatcaggtc
     4981 cgcttgataa tcgatgatcg gttggatgtt tgtcttgcgg ttgatgctgc tggtctgcat
     5041 attggagatg acgaactccc agtttcggta gctcgcaaag tcttgggtcc tgaaaaaatc
     5101 ctcggtgtca ccgctaaaac ggttaaaaga gctctggaag cggagaaatc aggtgcagat
     5161 tacttgggga caggagccat ttttccgaca accaccaagg aaaatgcacc catcaccctg
     5221 atttcaacct tgaaaacaat ttgtcaaacg gttgccattc cagtagttgc tattggcggc
     5281 ttgacatcag agaatattga ccaacttatc ggcacgggta tagctggtgt agctgttgta
     5341 cgtgatttga tgcaagcaga agatattgag gcaaaaacgc aagccttttt aacaaagttg
     5401 catgacattc tttcctaatt aatactcaat gaaaatcaaa gagtaaacta gaaagctagt
     5461 cgcaggttgc tcaaagtact gccttgaggt tttagataag actgacgaag tcagctcaaa
     5521 acagtgtttt gaggttgcag atggaagctg acgtagtttg aagagatttt cgaagggtat
     5581 aaaaactctc taattccctt aaagtgggaa ctagagagtt tttttcttaa tctttaaaag
     5641 ttgtatggtt gactggacca gaaccatgac cgagttgagg ggcatcttga atagcttttg
     5701 tgataaaggc cttggcctta taaactgcct ggtaaagact cttgcccttg gctaattcag
     5761 cagtaatcac tgcagcaaag gtacatccag taccatgggt gtgacaggtt tgaattcgtg
     5821 ggctttccca gacaaattgt tcattcttgg taaagaggaa atctttagca ccacctttga
     5881 gatgtccgcc tttgataacc acagactgag gaccaaattc ttttaaaatc aggcgaccag
     5941 cacgctgcat gtcttcgggg tcatggattg aaaaaccaac aatctcttct gcttcaggaa
     6001 gatttggcgt aataatagtt gctagaggta gtaagtttgt tttgagatag tctctagcac
     6061 ttgagtcaat caaggcatct ccacttgtag caaccataac aggatcaagg acatagggac
     6121 aatccagttt tttaagatag ggttggatga tttccatgat ttcagtagta gccaacattc
     6181 cagtttttac agcctgaggt ggaatatcag aaaagacact ctccaattgg gctttcaaca
     6241 tttgaggaga aacgtgctcg attagctgaa cacctctggt attttgagcg acaagactgg
     6301 ttacaacagc cattccatag acatctctcg cttggaatga ctttaaatct gccataatgc
     6361 cagcaccacc actagggtca gtccctgcaa tggtcaaagc aacgggtaaa taagtcatct
     6421 aaaacctccc aaccaactga agtttgtatt cttaagaaca agctagtctg ttttagaaag
     6481 gcaatagaag actgctagtc cccattatca tttccacttc catcagctag cattatctag
     6541 attgagtcag agggatctaa cagtcgttaa tctcagcttt acgcacccct agtggttgtt
     6601 ttcttttttc tttagtatag catattttta ttgtttatat agtaatattt gactgcaaat
     6661 cgtttttcaa atgattaaaa ttctattttt cgaagaaaat attatttcta taattgacaa
     6721 atgtagattt taagagtata ctgatgagtg taattgacaa atgtagattt tggaggtatg
     6781 gtaatgcaga tttcagatgc agaatggcag gtcatgaaga ttatttggat gcagggggag
     6841 cagaccagta cagatttgat tagggttttg gcagagcggt ttgactggtc caagtccacg
     6901 gttcagacac ttttggctcg tctggttgag aaagagtgtt tgactcggaa aaaagaaggc
     6961 aagtcctttg tttattcagc ccttttaact ctagaccaaa gtcgggattt acttgtccaa
     7021 gatatcaaag acaaggtttg ttcccgtagg attaggaatt tgttggctga cttgattgta
     7081 gaatgtgaat ttactcagac tgacttggaa gacttggaag ctgtgatttc agagaagaaa
     7141 tcaagtgctg taacagaagt aagatgtaat tgtatgtaaa ggagacgtca tgttaaatag
     7201 tattgtaacc attatttgta ttgcccttat cgcatttatc ttgttttggt ttttcaaaaa
     7261 gcctgaaaaa tctggacaaa aagcccagca aaaaaacgga taccaagaga ttcgagtgga
     7321 agtcatggga ggctatactc ctgagttgat tgtcctaaag aaatcagtgc cagcacgcat
     7381 tgtctttgac cgcaaggatc cttcaccatg tctggatcaa attgtttttc cagattttgg
     7441 tgtacatgcg aacctgccaa tgggggaaga gtatgtagtg gaaatcatgc ctgaacaggc
     7501 tggagagttt ggctttgctt gtggtatgaa catgatgcac ggcaagatga ttgtagagta
     7561 ggtggagact atgacagaaa ttgtgaaagc aagcttagaa aatggcattc aaaaaatccg
     7621 tatccgagct gaaaaaggct atcatccagc ccatatccag cttcaaaagg gaattccagc
     7681 tgagattacc tttcatcgtg ctactccttc aaactgttat aaggaaattc tgtttgaaga
     7741 agaaggtatc ttggaaccaa tcggcgtaga tgaggagaaa gtcattcgtt ttacgcctca
     7801 agaattaggg cgacatgaat tttcttgtgg catgaagatg caaaagggaa gctatacagt
     7861 cgttgagaag actcgaaaat ctctatctct cctgcaacgt ttttggatta ctagtatctt
     7921 tactgtgcct cttgtgattc tcatgattgg gatgttggca ggtagcatta gtcatcaagt
     7981 catgcattgg ggaacctttt tagcaacaac gcctattatg ttagttgcag gtaagccata
     8041 tatccagagt gcttgggcca gttttaaaaa gcacaatgcc aacatggata ccttggttgc
     8101 gctgggaact ctagtggctt atttctatag cctagttgct ctctttgctg gtctccctgt
     8161 ttacttcgaa agtgctggat ttatcctctt tttcgttctt ttgggagcag tttttgagga
     8221 aaaaatgaga aaaaatacgt cccaagctgt ggagaaatta ctggacttgc aagctaaaac
     8281 cgcagaagtc ttgagtgatg atagttatgt ccaagttcct ttggaacaag tcaaggtagg
     8341 cgacctgatt cgagtgcgtc ccggtgaaaa gattgctgtt gatggtgtcg tagtagaagg
     8401 tgtctctagt attgacgaat ccatggtgac aggtgagagt ctgcctgtgg acaagacagt
     8461 tggagatact gtcattggct caaccatcaa tcatagtgga acgcttgtct ttagagcaga
     8521 aaaagttggc tcagagactg ttttggctca gattgtggat tttgtgaaga aagctcagac
     8581 aagtcgtgcg ccgattcagg acttgacgga taagatttca gggatttttg tcccagtagt
     8641 tgtcatttta ggaatcatga ccttttgggt ttggttcgtc ttgctcaggg atagtgtggt
     8701 cgtgcttgga gctagctttg tgtcctctct tctctacgga gtggcggttt tgattatcgc
     8761 ctgtccttgt gccttgggac ttgcaacacc gacagccctt atggtgggga caggacgtag
     8821 tgccaagatg ggggttctcc tcaaaaatgg aactgtctta caggaaatcc agaaagttca
     8881 aactcttgtc tttgataaga ccgggacttt gacggaaggg aaacctgtgg taacagatat
     8941 catcggtgac gaagtagaag tgtttggatt ggcagcctcc ttggaagatg cttctcaaca
     9001 cccactggct gaggctatcg ttaagcgagc gagtgaagct ggacttgagt ttcaaactgt
     9061 tgaaaatttt caggccttgc acgggaaagg tgtttcaggg cgaatcaatg gaaaacaagt
     9121 tttacttgga aatgctaaaa tgctggatgg catggatatt tctaatactt atcaagataa
     9181 actagaagaa ctagaaaaag aagctaagac agttgtgttt ttagctgttg acaatgaaat
     9241 caaaggcttg cttgctttgc aagatattcc taaggaaaat gctaagctag ccatcagtca
     9301 gctaaaaaaa cgtggtctcc gaacagtcat gctgacagga gacaatgctg gtgtggcgcg
     9361 tgctattgca gatcaaatcg gaattgaaga ggtcattgca ggcgtcttgc cagaagaaaa
     9421 agcccatgaa atccataaac tgcaacagtc agggaaagta gcctttgttg gggacggtat
     9481 caatgacgct cctgccctta gtgtagcaga tgtggggatt gctatgggag ctggaacaga
     9541 tatcgccatc gagtcagcag atttggtgtt gacaaccaat aatcttttag gagtggttcg
     9601 tgcctttgat atgagtaaga aaacctttca tcgaattcta ctcaatcttt tctgggcttt
     9661 tatctacaat gttgtcggaa ttccgattgc agcaggagtc ttttcaggtg ttggactggc
     9721 tctcaaccca gaattggcag gtctagccat ggcctttagt tctgtatctg ttctgaccag
     9781 ttcccttctg ttaaatttta gtaaaataga ctaaaagtaa tgactagatt tctttgttat
     9841 aaaacagaaa tgacagaact ttaaaaactg tattagaata gagaatagag agttttgagc
     9901 agatttttag aaaagtcagc ataaatatga tacagtggaa tagtaaaaat ttggagaacg
     9961 tttccaattc tatgtaatcg tattctccaa atctaaaaaa attgaaggag agttatcatt
    10021 atgactcaag ggaaaattac tgcatctgca gcaatgctta acgtattgaa aacatggggc
    10081 gtagatacaa tctacggtat cccatcagga acactcagct cattgatgga cgctttggct
    10141 gaagacaaag atatccgctt cttacaagtt cgccacgaag agacaggtgc tcttgcagcg
    10201 gttatgcaag ctaaattcgg cggctcaatc ggggttgcag ttggttcagg tggtccaggt
    10261 gcgactcact tgattaacgg tgtttacgat gcagctatgg ataacactcc attcctagcg
    10321 atccttggat cacgtccagt taacgaattg aacatggatg ctttccaaga gcttaaccaa
    10381 aacccaatgt acaacggtat cgctgtttac aacaaacgtg tagcttacgc tgagcaattg
    10441 ccaaaagtaa ttgacgaagc ctgccgtgct gcaatttcta aaaaaggtcc agctgttgtt
    10501 gaaattccag taaacttcgg tttccaagaa atcgacgaaa actcatacta cggttcaggt
    10561 tcatacgaac gctcattcat cgctcctgct ttgaacgaag ttgaaatcga caaagctgtt
    10621 gaaatcttga acaatgctga acgcccagtt atctatgctg gatttggtgg tgttaaagct
    10681 ggtgaagtga ttactgaatt gtcacgtaaa atcaaagcac caatcatcac aactggtaaa
    10741 aactttgaag ctttcgaatg gaactatgaa ggtttgacag gttctgctta tcgtgttggt
    10801 tggaaaccag ccaacgaagt ggtctttgaa gcagacacag ttcttttcct tggttcaaac
    10861 ttcccatttg ctgaagttta cgaagcattc aagaacactg aaaaattcat ccaagttgat
    10921 atcgaccctt acaaacttgg taaacgtcat gcccttgacg cttcaatcct tggtgatgct
    10981 ggtcaagcag ctaaagctat ccttgacaaa gtaaacccag ttgaatcaac tccatggtgg
    11041 cgtgcaaacg ttaagaacaa ccaaaactgg cgtgattaca tgaacaaact cgaaggtaaa
    11101 actgagggtg aattgcaatt gtatcaagtt tacaatgcaa tcaacaaaca tgctgatcaa
    11161 gacgctatct actcaatcga cgtaggtaac actactcaaa catctactcg tcaccttcac
    11221 atgacaccta agaacatgtg gcgtacatct ccactctttg cgacaatggg tattgccctt
    11281 cctggtggta tcgctgctaa gaaagacaat ccagatcgcc aagtatggaa catcatgggt
    11341 gacggagcat tcaacatgtg ctacccagac gttatcacaa acgttcaata cgaccttcca
    11401 gttatcaacc ttgtcttctc aaatgctgag tacggcttca tcaagaacaa atacgaagat
    11461 acaaacaaac acttgtttgg tgtagacttc acaaacgctg actacgctaa aattgcggaa
    11521 gctcaaggag ctgttggatt cacagttgac cgtatcgaag acatcgatgc agttgttgca
    11581 gaagctgtta aattgaacaa agaaggtaaa actgttgtca tcgatgctcg catcactcaa
    11641 caccgtccac ttccagtaga agtacttgaa ttggatccaa aacttcactc agaagaagct
    11701 atcaaagcct tcaaggaaaa atacgaagca gaagaactcg taccattccg tctcttcttg
    11761 gaagaagaag gattgcaatc acgcgcaatt aaataattcc tctcgccgaa aatcaaatat
    11821 gaaacttgta gactttattc tttgattttc atagagcaga actttaaaag atcggagcaa
    11881 tccggtcttt ttatggagga gagtaaatga atttaaatca attagatatt atcgtttcaa
    11941 atgttcccca agtctgtgct gacttggagc atatcttgga taaaaaggca gattatgctg
    12001 atgatggttt tgctcagttt acgattggta gtcactgcct tatgttgtca caaaatcatt
    12061 tggttccttt ggaaaacttt cagtcaggaa tcattattca tatcgaggtt gaggatgtag
    12121 accagaacta caaacggttg aacgagcttg gtataaaggt tttacacgga ccaactgtaa
    12181 ccgattgggg aacagagtcc ttattagttc aaggacctgc tggtctagtg cttgattttt
    12241 atcgtatgaa ataggtcgcg tagtcatttg acgttaacct tagctatttt taaaatagaa
    12301 attgagaata tgaactattg aaagatcgga gcaatccggt ctttttatat aaagaaattg
    12361 catataatac tcatctgctt tcaaaaagta ttctagtcca tctctgatta acgatggact
    12421 ttatcacctc cttctccagt ccttgtatga catcttggag ttgattcatg acatcttcca
    12481 aagttcgaaa ggctttattc ttaaatccac gtttatgaat ctctttccac acttgttcga
    12541 tgggttcatc tctggtgtgt atggaggaat aaatgcaaaa ccaatattag tcggagtctt
    12601 tataagattt gcccatcaga cgaaatagaa tgatttgaag gcatttatga tatttagctg
    12661 tacgagagtc tttaaaaagt gttttgatgg tttggatttc ttctttagtt gatttcatat
    12721 cactattata taatgctttt tgattttagt atagtattat cgtagaaaat ggactcggtg
    12781 ctatggatac accggatgag aatggttatg tagcagatga ctaccggatt acttacttag
    12841 aggcccacat caaggccatg cgagatgcca tttacaaaga cggggttgac ttgcttggtt
    12901 atacgacatg gggctgtatc gattcggttt cagctggcac aggggaaatg aataagcgct
    12961 atggctttat ctatgtagat cgagataatg taggaaatgg gactctcaaa cgtagcaaga
    13021 agaagtcctt ctattggtac atgtcattta tagcaatggt gtaagcatcg agtagagcac
    13081 atttgtttac tatttttata aaatcttctc cctgctttat aagatgtaaa aaagagttca
    13141 aatggaactc tttttttgct ataatgaggg gagaaaaatc agacaggaga ttaagatgtc
    13201 agaaccatta tttttacaat cagttatgca agaaaaaatc tggggtggta ccaagctacg
    13261 tgatgagttt ggctacgaca tcccaagtga aaaagtcgga gagtactggg ctatctcagc
    13321 tcatctaaat ggtgtttcta aagttgccaa tggtcgttac gagggaacag atcttgctac
    13381 tttgtatgcg gaacaccgtg aattatttgg caatcgtcct gagcctgtat ttccgctttt
    13441 gactaagatt ttggatgcta acgactggct cagcgttcaa gttcacccag acgatgctta
    13501 tggactagag catgaaggcg aacttggaaa aacagaatgc tggtacatca tcgctgcgga
    13561 tgaaggttca gagattatct acggccacaa tgccaagtca aaagaagaac tccgccagca
    13621 aatcgaggac aagaactggg ataccttgct taccaaagtc cctgtcaagg ctggagattt
    13681 cttctatgta ccaagtggca ccatgcatgc tatcggtact ggcatcttga ttcttgaaac
    13741 ccaacagtct agtgatacaa cttaccgcgt ctatgacttt gaccgaaagg acgataaggg
    13801 caacttgcgt gaacttcacc ttgaaaaatc catcgatgtt ttgaacattg gcgagccagc
    13861 aaatagccgt cctgtaactg ttaaagcaga taatttgcgt tccactttac ttgtttctaa
    13921 tgatttcttc gcagtttaca agtgggaaat cactggaaaa gttgattttg agaagacagc
    13981 tgattacagt ttgtttagta ttttagctgg ccaaggtcaa ctgactgttg atggaaaaaa
    14041 ctatccaatc caaaaaggga gccactttat cctaccaagt gatgttgaat cttggacttt
    14101 agaagggcaa gatttagaat taattgttag tcatccataa aaaagaaagg gcctgagttc
    14161 attactcaag tccttttgat tacataaatt gggcgatgaa gaccacgatg atgattggaa
    14221 tgacgaaacg aagaaggaag agccagactt ggaacagtcc ttgtttccat gctctttcat
    14281 cgagatggag ttcctccatt gcaagagcct ttttaaagat atagcctgta aaaagtgaaa
    14341 ggcagagagc tccaaatggc atgagaagat tggaaaccaa gaaatccatg gcatcaaaga
    14401 aagttttacc aaagatgtga acatccgcca taactccata ggatagggct gaaggaatgc
    14461 caaagacaaa ggtcaaaatt cctaaaataa cactccattt ggcacgtttg ctgttgtcct
    14521 gattggtgat attgtctaca ttgatttcca gcataacgac agaagaagtg acagtcgcaa
    14581 agaggaagag gacatagaaa atggttccaa aaggcatctt gtcaaagagt tgaggcaaga
    14641 cgataaagac caagctcggt cccccttcag actggatatt gaagggggac cgagcttgga
    14701 aaatggctag acctgccatg atggataccg agatattcat ggctacgatg gagattcttg
    14761 actggaccag attggttttc ttgtccaagt aagaagcata ggtcaacatg gctgtaaccc
    14821 ctagtgagag ggcaaagaaa gattgtctca gagcatagag gagaccagcg ctagtcagtt
    14881 ttgaaaagtc tggtttgagg aagtaaagaa ccccttccat ggcatttgac aagctgagag
    14941 agcgaccgat gataacaaca aagatgataa agagcagggg catcatgact ttcgaagctc
    15001 tttcaatccc tttttgaacc ccacgtgata caatgaagat attcaatagg ataaaggccg
    15061 cttgagctcc tagggcaatg gctggatttg aaatgatca
//
LOCUS       DAVKWZ010000049        13816 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000049 DAVKWZ010000000
VERSION     DAVKWZ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13816
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.049"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(20..2179)
                     /locus_tag="VW854_001611"
     CDS             complement(20..2179)
                     /locus_tag="VW854_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEV0262823.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNAPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGVYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLTYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWNSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(2176..2772)
                     /gene="recU"
                     /locus_tag="VW854_001612"
     CDS             complement(2176..2772)
                     /gene="recU"
                     /locus_tag="VW854_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEV0262824.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            2838..3365
                     /locus_tag="VW854_001613"
     CDS             2838..3365
                     /locus_tag="VW854_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEV0262825.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            3435..3764
                     /gene="gpsB"
                     /locus_tag="VW854_001614"
     CDS             3435..3764
                     /gene="gpsB"
                     /locus_tag="VW854_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEV0262826.1"
                     /translation="MASIIFSAKDIFDQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            3785..4165
                     /gene="rnpB"
                     /locus_tag="VW854_001615"
     ncRNA           3785..4165
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VW854_001615"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4250..5407
                     /locus_tag="VW854_001616"
     CDS             4250..5407
                     /locus_tag="VW854_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEV0262827.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQIENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVERELELDIMGCDIDARMVEIAKANA
                     QVAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            5420..6814
                     /gene="mapZ"
                     /locus_tag="VW854_001617"
     CDS             5420..6814
                     /gene="mapZ"
                     /locus_tag="VW854_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEV0262828.1"
                     /translation="MSKKRRNRQKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVEIQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLNQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKPAIV
                     DGVLDANAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            6890..8314
                     /gene="gndA"
                     /locus_tag="VW854_001618"
     CDS             6890..8314
                     /gene="gndA"
                     /locus_tag="VW854_001618"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEV0262829.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPATFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            8326..9015
                     /locus_tag="VW854_001619"
     CDS             8326..9015
                     /locus_tag="VW854_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0262830.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLMLLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVLIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            9113..10135
                     /gene="cbpC"
                     /locus_tag="VW854_001620"
     CDS             9113..10135
                     /gene="cbpC"
                     /locus_tag="VW854_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HEV0262831.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDNLFDNQPVNEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     EGHWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDASGKML
                     TDWQKVNGKWYYFGSSGSMATGWKYVRGKWYYLDNKNGDMKTGWQNLGNNWYYLRSSG
                     AMVTGWYQDGSTWYYLDAENGDMKIGWTKVNGKWYYLNSSGAMVTGSQTIDGKVYNFA
                     SSGEWI"
     gene            10154..11152
                     /gene="cbpJ"
                     /locus_tag="VW854_001621"
     CDS             10154..11152
                     /gene="cbpJ"
                     /locus_tag="VW854_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpJ"
                     /protein_id="HEV0262832.1"
                     /translation="MKLLKKTMQAGLTVIFFGLLATNTVFADNSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDSLSGEMVVGWQYIPFPSKDSTIGPYPNGMRLEGFPNS
                     EWYYFDKNGVLQEFVGWKTLEIKTKDSVGRKYGEKREDSEDKEEKRYYTNYYFNQNHS
                     LETGWLYDQSNWYYLAKTEINGENYLGGERRAGWINDDSTWYYLDPTTGIMQTGWQYL
                     GNRWYYLRSSGAMATGWYQEGTTWYYLDQPNGDMKTGWQNLGNKWYYLRSSGAMVTGW
                     VKDDSTWYYLNAGNGDMKTGWFQVNGRWYYAYSSGALAVNTTVDGYSVNYNGEWVR"
     gene            complement(11270..12502)
                     /locus_tag="VW854_001622"
     CDS             complement(11270..12502)
                     /locus_tag="VW854_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV0262833.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALWIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEISHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLLALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLTLITSILCQAYIVIFMTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(12545..13507)
                     /locus_tag="VW854_001623"
     CDS             complement(12545..13507)
                     /locus_tag="VW854_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEV0262834.1"
                     /translation="MTSSTEFGNFVSNLSFQHGIAGLLFPLNKLYPPELDSKILSIIK
                     KAVTIRTTHTYEYQYSLLFGDAGYLWLLLHLFSISKNQYYLQLANVTAKKLIENYDTL
                     EEIDFALGKSGVLLSLIKYYQFTNDNTLKIFIHNSIGEIYHYFLQRDTAKESILDYSF
                     AHGYCGIAYALFAYSKVLEPSMFYNDLHTFHTELKKLLEKVTSNTENLGNLQLSWCKG
                     ISGIILYLCMYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLM
                     KKIQQVILACSERDDHGLLMFQGDSGKADLFDFGIGSMGYIGVY"
BASE COUNT         4203 a         2368 c         3145 g         4100 t
ORIGIN      
        1 aaaatctggg tgataaatgt tatggttgtg ctggttgagg attctgattt tgttgatcag
       61 gggttgtatt tgattgttgc gtattattgt taggattggt agtcgtacta ttatttgtgc
      121 ttggagtggt tgagctagac tgtgaagttg aactatctga tgatgagctt gaactttcag
      181 ttgatggggg ttgttgtgga gcaggtgagt tccacgtaga acgagcacca tttttaaata
      241 cgaattctcc atttctgtag agcccctctg gtatattcca atcttctgga ttgcttcctt
      301 cagacaggta ggtcatcata gagcggtaaa ctttggcagc gaccgtaagg ccattgccta
      361 caagtggtgt cagacggtta gaatagcctg tccatacagc cattgaatat ttacgcgtat
      421 agccagcaaa tagttcatca ggtgctacaa attgagaggt cttgatgtgg ttttcaattt
      481 cctcgtctgt atagttagag gttcctgttt taccagcctg aggaagccaa gcaagatagg
      541 catttcgtcc agttccataa gtcaagactg ttttcatcat gtcggtcatc atataggctg
      601 tcgtttcttt catggcacga gttccgacat tagagaactc tttttcactc ccatcactaa
      661 agacgacttt atggatatac attggtttat agtaagttcc accatttgca aaggcagcgt
      721 aagcagcagc catcttttca ctacttgctc catatttttt gtctgattcg gttgtgttac
      781 ttgaaatggc atttgagtag tgaatacttg ggtagtcgat tccgagacca tttaggaaag
      841 tcttggcgcg gttgagtccg accttgttta gagtttccac ggctgggacg tttcgcgatt
      901 gttgcagggc gtattgcaag gtgatgttgc caaagtagcc cctatcccag ttataaacag
      961 gagtatttgt cccagggtaa ttatagggct catcgtgaac gatagtagca gttgaatcgt
     1021 agacaccgta ctccaaggca ggagcatagt ctgtgatcgg tttcatagtt gatccccagt
     1081 cgcggtttgt ttctactgct tggttaattc cgaaggaaac attacttgac tgatggcgtg
     1141 ctcctagctg ggcaatgact ttaccgttag aaacatcaac aatggtagaa gcgacttgca
     1201 attcatcgtc tggataggca acgtattcgt ctgtattgta aatatcccac agatgttttt
     1261 gagcttcttg gtctacattt gtgtagacat ccatcccagt tgtgagcagg ttatagcctg
     1321 tttcttcttc aacttgattg atgacttcct tgaggtaatt atccatgtaa gcagggtaat
     1381 tacttgctga tttgagactt tgtagtccat cagtaattgg tgcattgact gctttctcat
     1441 actgttcagc agagatgtag ccttgatttt tcatttcaga taagaccaag tttcggcggt
     1501 cttgggctgc ttctggatgt gaataggggt catattggtt tggtgcctga ggcattccag
     1561 ccagcaaggc taactgaggt aaacttaaat tattgaggtc tttaccatag tagttttgag
     1621 ctgctgtctg cattccatag ttcccattag acatgtagac cttatttata tagtaggtca
     1681 agatttcttg cttggttgct ttttgttcta actgaatcgc taaccaagct tcctgagcct
     1741 tacgagaaat agtctggtcg gaagtcgaag ttgaaaagta agtcaactta atcaactgtt
     1801 gggtgagagt tgatccacct tggagggaat tgctttgcag attgcgcaag aaagctccca
     1861 ggatacggat ggtatcaatc cccctgtggt cgaagaagcg atggtcttcg atagaaacga
     1921 ttgccttaac caaatctgtg ggaatatcat tagcttgggc attgacgcgg cgttcagaac
     1981 ccaagtcagc aatgagttga tttttattgt cgtagatttt actagaagtt gttgcaacta
     2041 gtttactctc ggataggcta ggagccttgc taacgtagta gaaaaaaact cctccgccta
     2101 agacaatggc tgcgataacc aagcttaaga agctaatgct cagatacttg attaggcgca
     2161 gaatcgttgg tttgttcatc ttgttttacc acctaataaa tgttctttga taacattgag
     2221 ataaggaatt tgagggaagg caccagcctt gatttcatat ccatattctc gaatatattc
     2281 aagtggcatt gatttttgtc ccttatcttg atgatagaag cgaatcaaat cgaatgccgg
     2341 caataagtag gtttcttgct gagaagaaaa gtgaagaagg acaaagcaga ttccttgttg
     2401 ggcaaggact tgttccatat gctgaatctg atgtggatga aaatttttca tcggaatcgc
     2461 acgtttttgt tttgtttcct tgacttcaaa gtcgatgtaa tatccattat aaacgccaga
     2521 atagtccgtc gttgaagctt gtcgaaaata ggcttcaaca atcttggcac gacttcgttg
     2581 tggatagtcc acttgtacga tttgaatagg agttggtttc ttatgtataa cagccaagcc
     2641 ctgagacaaa tagtagtcgt tggtagcatt gatcatcttt tcaaaagaca ttcctcgatt
     2701 tgcgaaattt ttgggttgag aaagagatgt ttgtcttttt tgtgatgaaa ctttatgtgg
     2761 atagttgacc ataattctcc ttattggtac aataacatca ctttattata ccataatttg
     2821 cacagaaagg gttaaaaatg gctacagctt tggttttagg ctattctgct tttgatttgg
     2881 gtttattttc tgataaggat cctcgtctta aattgattaa aaaagcgata cgtaaagatt
     2941 tagaggctat ggcagcagat ggggtgtctt ggcttgtgtt tacaggaagt ttgggttttg
     3001 aatattgggt tttagaggtt gctcaggaga tgaaaaccga atacggtttc cagttggcca
     3061 ccatttttgc ttttgaaacc catggggaaa attggaatga aggcaatcag atgaaactga
     3121 gtcgctttaa gcaggtagac tttgtcaaat atgcttatcc tcgctatgaa cacaaggggc
     3181 agttaagaga ttaccagcag tttttgctgg aaaacacgac tagttcctat cttttttatg
     3241 atgaagaaaa tgaaacaaaa ctagcttatt tttaccaaaa gatgaaaaat caagaggact
     3301 attttataaa gagattaaca tttgatcaat taaatgaact tgctgaaaat ttttccgaaa
     3361 attgaagctt tgaccttgat ttttgtttgc ctttttttat ataataatac tagcaagcga
     3421 gaatggagag agacatggca agtattattt tttcagcgaa agatattttt gatcaagagt
     3481 ttggacgtga agtccgtggc tataataaag tagaagttga cgagttttta gacgatgtca
     3541 tcaaggacta tgaaacctat gctgccttgg tcaagtcact tcgtcaggaa attgcggatt
     3601 tgaaggaaga attaactcgt aaaccgaaac cttcaccagt tcaagcagaa ccccttgaag
     3661 cggcaattac aagttctatg acgaattttg atattttgaa acgcctgaat agattggaaa
     3721 aagaagtttt tggtaaacaa attttagata actcagattt ttaagtagtt atttgagatg
     3781 tgcaattttt ggataatcgc gtgaggagaa ttgcttctca tgaggaaagt ccatgctagc
     3841 acaggctgtg atgcctgtag tgtttgtgct aggcgaaacc ataagcctag ggacgagaaa
     3901 tcgttacggc agttgaaatg gctaagtcct tggataagcc agagtaggct tgaaagtgcc
     3961 acagtgacgg agtctttctg gaaacagaga gagtggaacg cggtaaaccc ctcaagctag
     4021 caacccaaat tttggtcggg gcatggagta cgcggaaacg aacgtagtat tctgactgct
     4081 atcagttaga gctgttagtg gtagacagat gattatcgaa ggaagtggtc ctagtcactt
     4141 ctggaacaaa acatggctta tagaaaattg catataggtt ggggctgaga aattttctca
     4201 acctcatttt ttaaagtgga catatagaaa ggtcttgcaa gactgtaaca tgaaaaaaga
     4261 atttaattta attgcaactg tggcagcagg gcttgaggct gtcgttggtc gtgaagtgcg
     4321 agagttgggc tacgattgtc agattgaaaa tggacgtgtt cgttttcaag gagacgtgag
     4381 agctattatc gaaaccaacc tttggcttcg ggcagcagat cgtatcaaaa ttatcgtagg
     4441 aacgttccct gctaagactt ttgaagagct atttcaggga gttttcgctt tagattggga
     4501 aaattattta ccacttggag ctcggttccc gatttcaaaa gctaaatgtg ttaagtccaa
     4561 acttcacaat gagcccagtg ttcaggctat ttctaagaaa gctgttgtca agaaattgca
     4621 gaaacactat gctcgcccag aaggggttcc tctgatggag aatggcccag agtttaagat
     4681 tgaggtctct attctcaaag atgtggcaac tgtcatgatt gatacgaccg ggtctagcct
     4741 ctttaaacgt ggttatcgta ccgaaaaagg tggcgctcct atcaaggaaa atatggcagc
     4801 agccatttta caactttcta actggtatcc agacaagcct ttgattgatc cgacctgtgg
     4861 ttcggggact ttctgtattg aggcagttat gattgctaga aagatggcgc caggtcttcg
     4921 tcgctctttt gcatttgagg aatggaactg gatcagcgat cgcttgattc aagaagtgcg
     4981 cacagaagcg gctaaaaaag tagaacgtga gcttgagctg gatatcatgg gctgtgatat
     5041 tgatgctcgc atggtggaaa ttgctaaggc caatgctcag gtggctggtg ttgcaggaga
     5101 cattactttt aagcagatgc gcgtgcagga tttacgttcc gataaaatca atggagtaat
     5161 catttccaat ccgccttatg gtgaacgttt gtcagatgat gcaggggtga ccaagctcta
     5221 tgctgagatg gggcaagtat ttgcaccgct gaaaacttgg agcaaattta tcctgactag
     5281 tgatgaagct tttgaaagca agtatggtag ccaagcagat aagaagcgta agttatacaa
     5341 cggaaccttg aaagtggatc tgtatcaata ttttggtcag cgtgtcaaac ggcaagaggt
     5401 aaaatagaaa gggatactca tgagtaaaaa aagacgaaat cgtcaaaaaa aagaaggtca
     5461 agaaccgcaa tttgattttg atgaagcaaa agagctaaca gttggtcaag ctattcgtaa
     5521 aaatgaagaa gtggaagcag gagtcttgcc tgaggattcc attttggaca agtatgttaa
     5581 gcaacacaga gatgaaattg aggcggataa gtttgcgact cgtcaataca aaaaagagga
     5641 gttcgttgaa attcagagtc tggatgattt aattcaagag atgcgtgagg ctgtagagaa
     5701 gtcagaagct tcttcggagg aagttccatc ttctgaagac atcttactac ccttgcctct
     5761 ggacgatgag gagcaaggct tggatcctct attgctagat gatgaaaatc caacagaaat
     5821 gactgaagaa gtggaagagg agcaaaacct ttctcgtctg gatcaagagg actcagaaaa
     5881 gaaaagtaaa aaaggcttta ttttgaccgt tttggcgctt gtatcagtaa ttatttgtgt
     5941 cagtgcttat tatgtctacc gtcaagtggc tcgttcgacc aaggaaattg aaacttctca
     6001 atcaactaca gccaatcaat cggatgtgga tgattttaat acactttatg acgcctttta
     6061 cacagatagc aataaaacgg ctttgaaaaa tagccagttt gataaactga atcaactcaa
     6121 gactttactt gataagctgg aaggtagtcg tgaacatacg cttgccaaat ctaaatatga
     6181 tagtctagca acgcaaatca aggctattca agatgtcaat gctcaatttg agaaaccagc
     6241 tattgtggat ggtgtgttgg atgccaatgc caaagccaaa tcggatgcta aatttacgga
     6301 tattaaaact ggaaatacgg agcttgataa agtgctagat aaggctatca gtcttggtaa
     6361 gagccagcaa acaagtactt ctagctcaag ttcaagtcaa actagcagct caagttcaag
     6421 tcaagcaagc tcaaatacga caagtgagcc aaaaccaagt agttcaaatg agactagaag
     6481 tagtcgcagt gaagtcaata tgggtctctc gagtgcaggg gttgctgttc aaagaagtgc
     6541 cagtcgtgtt gcctataatc agtctgctat tgatgatagt aataactctg cctgggattt
     6601 tgcggatggt gtcttagaac aaattctagc gacttcacgt tcacgtggct atatcactgg
     6661 agaccaatat atccttgaac gtgtcaatat cgttaacggc aatggttatt acaacctcta
     6721 caagccagat ggaacctatc tctttaccct taactgtaag acaggctact ttgtcggaaa
     6781 tggcgctggt catgcggatg acttggacta ctaagcagtc gttacaaaat tctttctttt
     6841 caaaagtaaa aatgataaaa taaaacaaat gaaacaagag gagtgtcaaa tgacaaaagc
     6901 taactttggt gtcgtaggta tggccgtaat gggtcgtaac cttgccctta atattgaatc
     6961 tcgtggttac acagttgcta tctacaaccg tagtaaagaa aaaacggaag atgtgattgc
     7021 ttgccatcct gaaaagaact ttgtaccaag ctatgacgtt gaaagttttg taaactcaat
     7081 cgaaaaacct cgtcgtatca tgctgatggt tcaagctgga cctggtacag atgctactat
     7141 ccaagccctt cttccacacc ttgacaaggg tgatatcttg attgacggag gaaatacttt
     7201 ctacaaagat accatccgtc gtaatgaaga attggcaaac tctggtatca actttatcgg
     7261 tactggggtt tctggtggtg aaaaaggtgc ccttgaaggt ccttctatca tgcctggtgg
     7321 acaaaaagaa gcctacgaat tggttgcgga tgttcttgaa gaaatctcag ctaaagcacc
     7381 agaagatggc aaaccatgtg tgacttacat cggtcctgat ggagctggtc actatgtgaa
     7441 aatggttcac aatggtattg agtacggtga tatgcaattg atcgcagaaa gctatgactt
     7501 gatgcaacac ttgctaggcc tttctgcaga agatatggct gaaatcttta ctgagtggaa
     7561 caagggtgaa ttagacagct acttgattga aatcacagct gatatcttga gccgtaaaga
     7621 cgatgaaggc caagatggac caatcgtaga ctacatcctt gatgctgcag gtaacaaggg
     7681 aactggtaaa tggacgagcc aatcatctct tgaccttggt gtaccattgt cactgattac
     7741 tgagtcagtg tttgcacgct acatttcaac ttacaaagaa gaacgtgtac atgctagcaa
     7801 ggtgcttcca aaaccagcta ccttcaactt tgaaggagac aaggctgaat tgattgaaaa
     7861 aatccgtcaa gccctttact tctcaaaaat catttcatac gcacaaggat ttgctcaatt
     7921 gcgtgtagcc tctaaagaaa acaactggaa cttgccattt gcagatatcg catctatctg
     7981 gcgtgatggc tgtatcatcc gttctcgttt cttgcaaaag attacagatg cttacaaccg
     8041 cgatgcagat cttgccaacc ttcttttgga cgagtacttc ttggatgtta ctgctaagta
     8101 tcaacaagca gtgcgtgata tcgtagctct tgcggttcaa gctggtgtgc cagtgccaac
     8161 tttctcagca gctattactt actttgatag ctaccgttca gctgaccttc cagctaactt
     8221 gatccaagca caacgtgact actttggtgc tcacacttac caacgtaaag acaaagaagg
     8281 aaccttccac tactcttggt atgacgaaaa ataagtaggt cagccatggg gaaacggatt
     8341 ttattacttg agaaagaacg aaatctagct cattttttaa gtttggaact ccagaaagag
     8401 cagtatcggg ttgatctggt agaggagggg caaaaagccc tctccatggc tcttcagaca
     8461 gactatgatt tgatgttatt gaacgttaat ctgggagata tgatggctca ggattttgca
     8521 gaaaaattga gtcgaactaa acctgcctca gtcatcatga ttttagatca ttgggaagac
     8581 ttgcaagaag agctggaagt tgttcagcgt tttgcagttt catacattta taagccagtc
     8641 cttatcgaaa atctggtagc gcgtatttcg gcgatcttcc gaggtcggga cttcattgat
     8701 caacactgca gtctgatgaa agttccaagg acctaccgca atcttaggat agatgttgaa
     8761 catcacacgg tttatcgtgg tgaagagatg attgctctga cccgccgtga gtatgacctt
     8821 ttggcgacac ttatgggaag caagaaagta ttgactcgtg agcaattgtt ggaaagtgtt
     8881 tggaagtatg aaagtgcgac cgagacaaat atcgtagatg tctatatccg ctatctacgg
     8941 agcaagcttg atgttaaagg ccaaaaaagc tacattaaaa ccgtgcgtgg tgttggatat
     9001 accatgcaag aatagataag cagttgcagt tgtgtaactg cttttttgag gagtttttat
     9061 atattgacat gcagtcgggt ctttgctaca atcgttttag gaggatatat agatgaaact
     9121 tttgaaaaaa atgatgcaag ttctactagc agtctttttc tttggtttgc tagctacaaa
     9181 tacggtattt gcgaatacca caggtggccg atttgttgat aaggataata gaaaatatta
     9241 tgtaaaagat gatcataaag caatctattg gcataaaata gacggtaaaa cttactattt
     9301 tggtgatatt ggagagatgg ttgtcggttg gcaatactta gaaattcctg gaacaggtta
     9361 tcgtgataat ttattcgata accaaccagt taatgaaatt ggccttcagg agaagtggta
     9421 ctattttgga caagatggtg ctttgctaga acaaacagat aaacaagtac tagaggcaaa
     9481 aacgtctgaa aatacaggaa aagtatacgg tgaacaatat cctctatctg ctgaaaagag
     9541 aacttattat tttgataata attatgctgt aaagacaggc tggatttatg aagagggtca
     9601 ttggtattat ttaaataagc taggaaattt tggcgatgat tcttacaatc cactaccaat
     9661 tggtgaagtt gctaagggtt ggactcaaga ttttcatgtt actattgaca ttgatagaag
     9721 caaacctgct ccatggtact acctagatgc ttcaggtaag atgcttacag attggcaaaa
     9781 agtaaacgga aaatggtatt attttggctc ctctggttct atggcaacag gttggaaata
     9841 tgtacgaggc aaatggtatt acttagataa taaaaatggc gatatgaaaa caggttggca
     9901 aaaccttggg aacaactggt actacctccg ttcatcagga gctatggtaa ctggctggta
     9961 tcaagatggt tcaacttggt actatttaga tgctgaaaat ggtgatatga aaataggttg
    10021 gacaaaagta aatggaaaat ggtattatct caattcctct ggagcaatgg ttacaggtag
    10081 tcaaactatc gatggtaaag tttataactt cgcttcatct ggtgagtgga tttaatgtta
    10141 gaggatatat aaaatgaagc ttttgaaaaa aactatgcaa gctggactaa cagtaatttt
    10201 ctttggtttg ctagctacga atacagtatt tgcagataac tctgaaggat ggcagtttgt
    10261 ccaagaaaac ggtagaacct attacaaaaa gggggacctc aaagaaacct actggcgagt
    10321 gattgatggt aagtactatt attttgattc tctatctgga gagatggttg tcggctggca
    10381 atatatcccg tttccatcta aagatagtac aattggtcct tacccaaatg gtatgagatt
    10441 agaaggtttt ccaaattcag agtggtacta cttcgataaa aatggagtgc tacaagagtt
    10501 tgttggttgg aaaacattag agattaaaac taaagacagt gttggaagaa agtacgggga
    10561 aaaacgtgaa gattcagaag ataaagaaga gaaacgttat tatacgaact attactttaa
    10621 tcaaaatcat tctttagaga caggttggct ttatgatcag tctaactggt attatctagc
    10681 taagacggaa attaatggag aaaactacct tggtggtgaa agacgtgcgg ggtggataaa
    10741 cgatgattca acttggtact atttagatcc aacaactggt attatgcaaa caggttggca
    10801 atatctaggt aatagatggt actacctccg ttcctcagga gcaatggcca ctggctggta
    10861 tcaggaaggt accacttggt attatttaga ccagccaaat ggcgatatga aaacaggttg
    10921 gcaaaacctt gggaacaaat ggtactatct ccgttcgtca ggcgccatgg tgactggctg
    10981 ggtgaaagat gactcaactt ggtattacct aaatgcaggt aatggagaca tgaagacagg
    11041 ttggttccag gtcaatggca gatggtacta cgcttatagc tcaggtgctt tggcagtgaa
    11101 tacgaccgta gatggctatt ctgtcaacta taatggcgaa tgggttcggt aatgacaggg
    11161 cgcttataat tatattagaa cactcgctta atcaaagaag tgactttgtt gtacttgacc
    11221 tgacgattgg ggagattaaa agcatgtgaa atctacacat gctttttcgt tagttatgag
    11281 tgttgaactt gactaaccat tgacaaaaga taagagcaat taaaataaga tacaaaaata
    11341 tcaatgctaa ctgcaccgaa acgcccgatg ctatggatag ggctaatagg gagagcaggg
    11401 acggggcgag aatgtccact gtgctcagag cagagcccac cgtccccatc ttgtcactgg
    11461 ggatatgggc aaagacagct gcttgtaggc gagggctgag aatgccgata atcgtagaac
    11521 tgatgaaact ggtcatgaaa attacgatat aggcttgaca aaggatacta gttattagag
    11581 tcaataggga aatctcacag aaaaccaaga ggtgcgtgaa acggatattt ttaaatagat
    11641 tactgctggt catattgcca aggatagctc cacaagacat gatgatggag agcagggcga
    11701 ggagttgccc aatgctcagt tgtgctagtg tctgttggat gaagcggagg gcaatcagcc
    11761 gaggggacac atccaagatg gcaatcatac tggtcgtcag aaacagtagt atgacggtat
    11821 cttttaacct caggagcaca gccagcgact gtttaagatg ttgaaaatat ttcttaaaac
    11881 tcagtgctgt atgtgatatt tcaattcttt tttcaacctc atacaaggta tgtcgaataa
    11941 catacaggcc caaaaaggca atgacaaaag tcagagtgtt gataagggaa atagtttgaa
    12001 tacttataac attgataagg aatgcgccag ccagattggc gacaatacgg acaaccctca
    12061 tcagagactg cctgaacccc atggcatcat gcaggtcgtc cttaattaca ctgatgtaga
    12121 gggcgttcat catgtaggca gacaggtagc tcaaggtgtc agagatgagg ttgatgatga
    12181 caatcataat gactgaaact aatgaaaagg gcgacaagac gagaaatact gtcaaaatag
    12241 cgtagagcag gattttgatt ttggcaatcc agagtgcgtg tttgactcta ttttcttgaa
    12301 aatctcctag aatcccgaga aagagttgcg atagtagggg aaccatctct gaaatggaaa
    12361 tcagtaaaat cgctagcgga gcgaaattgt actgagccac ataattgata atggcgaggt
    12421 agaaaatact gtcgccgaat gatgaaaaac agctgtttag cagcaactgt ctatatatgt
    12481 tgtttgtcca aaataatttc atacttttct ccttatgtct gcacatcgaa tgggaattta
    12541 ttatttaata gacaccaata taccccatgc ttcctattcc gaagtcaaac aaatctgctt
    12601 taccactatc tccttgaaac atcagtaaac cgtgatcatc tcgttcagaa catgctaaaa
    12661 ttacctgttg gatttttttc atcagtaatt tgttttgatt gtagacagtg gtttgtagta
    12721 agctagttat tccgtggcaa tatcctgtca tcatttttag atgatggtta aaaacaaatt
    12781 cttgatattt actaataata tctttgtttc cgtcacaatc gtacatacaa agatataaga
    12841 ttattccgga aattcctttg caccaagaaa gttgtaaatt tcctaaattt tcagtattag
    12901 aagtaacttt ttctaataat ttttttaatt cagtatggaa tgtatggaga tcattataga
    12961 acatagaagg ttctaagact ttagaatagg caaataaagc atatgcaatt ccacaatatc
    13021 catgagcaaa gctatagtct aaaatgcttt ctttggctgt atctctttgt aggaaataat
    13081 gataaatttc ccctatacta ttgtggatga aaattttaag agtattgtca ttggtaaatt
    13141 gatagtattt tattaatgat aataggacac cagattttcc caatgcaaag tctatttcct
    13201 ctaaagtatc ataattctct attaattttt tagcggtgac gtttgctaat tgtagatagt
    13261 attgattttt actgatagaa aataaatgta ggagtaacca tagatagcct gcatcaccaa
    13321 atagcagtga gtattgatat tcatatgtat gtgtcgttct aattgtcact gccttcttga
    13381 tgatagagag tattttagaa tccagttctg gggggtacaa tttatttaga ggaaacagta
    13441 gtccagctat tccgtgttgg aatgataaat tacttacgaa atttccaaat tctgttgaag
    13501 aagtgatcct tccttcagaa tttacagttt tatctagtaa aaattgtaga acactattac
    13561 attctgtttt gtaatcatat ttatgttcct ctattttttt aattttgact aaattatttg
    13621 tttgacttaa atttagtaga cattgtaacc aaaatagttg ctcatctgtg agctgacctt
    13681 gagtatgtga tagttctagt aattgaatag gatcctttag ttagattcaa tcaatctata
    13741 acacaacaac ataataatac ttttaggata gcttgtcaaa tattatagta aaatgagata
    13801 ataacaggat aaatcg
//
LOCUS       DAVKWZ010000050        13787 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000050 DAVKWZ010000000
VERSION     DAVKWZ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13787
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.050"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            13..936
                     /locus_tag="VW854_001624"
     CDS             13..936
                     /locus_tag="VW854_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEV0262835.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHDIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            996..1601
                     /locus_tag="VW854_001625"
     CDS             996..1601
                     /locus_tag="VW854_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV0262836.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFADKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQSFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEEFDWENL"
     gene            1619..2863
                     /locus_tag="VW854_001626"
     CDS             1619..2863
                     /locus_tag="VW854_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV0262837.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRNFKFKFELEVLEIDEVPMF
                     NQDEKWDESFQLRFLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGDHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(2980..3237)
                     /locus_tag="VW854_001627"
     CDS             complement(2980..3237)
                     /locus_tag="VW854_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEV0262838.1"
                     /translation="MDPKKIARINELAKKKKTEGLTLEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(3279..5315)
                     /locus_tag="VW854_001628"
     CDS             complement(3279..5315)
                     /locus_tag="VW854_001628"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEV0262839.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQSVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSELFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(5527..6444)
                     /gene="glyQ"
                     /locus_tag="VW854_001629"
     CDS             complement(5527..6444)
                     /gene="glyQ"
                     /locus_tag="VW854_001629"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEV0262840.1"
                     /translation="MSKKLTFQEIILTLQRFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATSPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRAKLLAEDAE"
     gene            complement(6638..7399)
                     /locus_tag="VW854_001630"
     CDS             complement(6638..7399)
                     /locus_tag="VW854_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV0262841.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMAPDLAVWKELYLYQLMDYECSQQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVLDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            complement(7672..8514)
                     /locus_tag="VW854_001631"
     CDS             complement(7672..8514)
                     /locus_tag="VW854_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV0262842.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQDQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     IAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERENLKTIAVQSGAPRVD
                     DVDF"
     gene            complement(8628..10019)
                     /locus_tag="VW854_001632"
     CDS             complement(8628..10019)
                     /locus_tag="VW854_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV0262843.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKYQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(10254..10631)
                     /locus_tag="VW854_001633"
     CDS             complement(10254..10631)
                     /locus_tag="VW854_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEV0262844.1"
                     /translation="MICQFIRDMLDLPAKNVTILEGSNIHVLPSMPYSAQDFYTSIDV
                     LAELDNGIQVIIEIQVHHQNFFINRLWAYLCSQVNQNLEKIRQREGDTHQSYKQIAPV
                     YAIAIVDSNYFSDDLAFHSFIVK"
     gene            10846..11823
                     /locus_tag="VW854_001634"
     CDS             10846..11823
                     /locus_tag="VW854_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV0262845.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLAG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFEKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            11820..12902
                     /locus_tag="VW854_001635"
     CDS             11820..12902
                     /locus_tag="VW854_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV0262846.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     ILRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKVILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(12966..13787)
                     /locus_tag="VW854_001636"
                     /pseudo
     CDS             complement(12966..13787)
                     /locus_tag="VW854_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         4045 a         3057 c         2515 g         4170 t
ORIGIN      
        1 ggagttggta gcttgactct tcgttcacat tctgaacggc taatggggac tactatcact
       61 atttcattag tggatgagca agccgatatc tttctccaaa agtcctttga cttgctcaaa
      121 gaacttgaat accgcttcaa cgccaatagt caagaatctg agttgatgga aatcaattat
      181 caagcaggaa tagcccccgt cacggttcat ccagacctgt ttgagctgat ttcacttggg
      241 ttagagcata gcctagcgct ctctagccac ctcaatatca gcattggtcc cttaattcaa
      301 acctggcgta tcggtttttc agatgccaag gtcgcccagc ctcaagagat tgaatcggtg
      361 ctgcctctaa tcaatcctca tgatatcgag ttagattctt ctacttctac tgtgttttta
      421 aaacagaaag gaatgaagat cgatctaggt tgtttagcca aaggatacag tgcggataag
      481 gttgcccaat ttcttaggaa agagggggtg acttctgcct tgatcaatct gggagggaat
      541 atcctgacca ttggaaaaaa tcaggcaaga ggggataacc catggcaaat cgggattcaa
      601 gacccagcca atcctagggg aaatcattta atgaccatcc ctgttgtcaa taaatctgtc
      661 gtgacttcag gcatttatga acgtcacctg accgtcgatg gacaagatta ccatcacatt
      721 tttgacagtc aaacaggata tcctgttgaa acggaactag cgagtctaac aatcatctct
      781 gataaatcag tcgatggcga aatctggacg actcgactct ttggagaaag acctgcttct
      841 atcctctggc aagtcgaaag tttggagggc atcgaagtta tcctcatcga taaagaaggc
      901 cacctaagct gttcttcagg aattcctact ctatagagac aactgtttca atggaatttt
      961 aaaattgtat tatgtaaaaa gagaaaggaa atctcatgtt aaaacttatt gctattgttg
     1021 gaacaaattc aaaacgttct acaaaccgtc aattgcttca atacatgcaa aaacactttg
     1081 ctgacaaagc tgaaattgag cttgttgaaa tcaaggccat tcctgtcttc aacaaaccag
     1141 ctgacaagca agtacctgct gaaatattgg aaattgctgc taaaatcgaa gaggcagatg
     1201 gcgttattat cggtactcct gagtatgatc actctattcc agctgttttg atgagtgctc
     1261 ttgcttggct atcttacggt atctacccac ttttgaacaa accaatcatg attacaggtg
     1321 cttcttacgg tacgcttggt tcatctcgtg cccaattgca acttcgtcaa atcttgaatg
     1381 ctcctgaaat caaggcaaat gttcttccag atgaattctt gctctcacac tctcttcaat
     1441 catttaaccc aagtggcgac ttggttgacc ttgatgttat caagaaattg gatgccatct
     1501 ttgatgactt ccgtatcttc gtgaagatta ctgagaaatt gcgcaatgcg caagaattgc
     1561 ttcgcaaaga tgcagaagaa tttgactggg aaaatttgta agataggaga ccgaaagaat
     1621 gaaatttgtt ggacttgttg gatcaaacta cgatcaatca tataaccgca aactcttgga
     1681 atttatccgt cgcaatttca aattcaaatt tgaattagaa gtacttgaaa tcgacgaagt
     1741 tccaatgttt aaccaagacg aaaaatggga cgaaagcttc caattgcgtt tcttgtataa
     1801 caagattaca cgtgctgatg gtgtcattat cgccactcct gagcacaacc acactatctc
     1861 agcttcactc aaatctgtac ttgaatggct ttcatacgaa gttcatccat ttgaaaacaa
     1921 gcctgttatg attgtgggtg catcatacta tgaccaagga acatcacgtg ctcaagttca
     1981 ccttcgtaaa atccttgatg ctccaggtgt taatgcctac acgcttccag gtaacgaatt
     2041 ccttcttggt aaagctaagg aagcttttga taacaacgga aatatcacca acgaaggcac
     2101 tgttaaattc cttgaaactt gcttagataa ctttgttaaa tacgtaggag tcgtttcgaa
     2161 attgaaaaaa ccaaaaccaa tcgaatcaga agacttggat tgtggaaaac caatcgctac
     2221 aaccatcaca gaagttgacc ctgacgatcc agaatgggta gaaaaagttg cagcaatcac
     2281 tggtgctgtt tctggtgata cctatgtcaa attggaccac ggtatcctaa cagttaacca
     2341 aattgatatg ttcttgaaag ctatgccatt tgaattgaca tacgctgacg acaacaacca
     2401 attcctctac tacaacaacg ctcaccaaga tccagacact atgttcgcta aacgtgtacc
     2461 acctcaatca ggtagccgta tgtcgactgt tcatggttct cttccaccag cacgtatgaa
     2521 gaacgtagag tgggttatcg gaacacttcg caacggaaac caagaatacg tccgtacgat
     2581 cgttccaggt tctcctgcag gtgttatcaa cactcacaac taccaagcaa tgtactatcc
     2641 tgatggatca tacgctggta tcaatgaaat tgtctttaac ttccaaccat ggcttgactg
     2701 gtacctaaaa gagactggtc aacgtttggt gggtggtagc ggaccatttg ctcctgctgc
     2761 cggagatcat ggagacgccg atgctacttc tggcgcttct gattcagggg atgctggagg
     2821 ccacggtggt gacgcagacg ctacagccgg tgcaagttac taatgatagt ttttaggaac
     2881 cattatggtt ccttttttag cgaaaaaaga ctagcaaaca caaactgcta gtctctttgt
     2941 tatcgtttca tctatctttg ttaaaaaaaa gagtacatat tatgaatttg ggtcatcaag
     3001 actacggcca tgtaatcctt tttcacgttg tacttggcgt agtttttctg gtgtaacatc
     3061 gtttccttct tcgtccacaa ttttgattcc ttcaatgtga tgacgaacag cgcgacgata
     3121 accttcgatg tactcctcac gtagtttggc ttgttccact ttttcttcta gtgttaagcc
     3181 ttctgttttt ttctttttag caagctcatt gatacgagca atttttttcg gatccatttc
     3241 ttctcctctg taataagata aagtccgttt atcaaatttt atttagtgtt aatttggtta
     3301 aaacaagcaa acttagctgc tttcttggtt agttgtgaca agattgccaa acgattttga
     3361 cggacagcct gatcttcagc cattaccata gtattttcaa agaaagcatc aatgactggg
     3421 ctaagcgcaa agagttgttt caattgctga cttgcaggtc ctgataaaac gagtgtttct
     3481 actgcttctg ccaaagcttt ttcttggtca ttctcaaata gttctgaatc aactgtagca
     3541 accccttctg ccttctcagc caggttaaag gcacgagaaa gtgattcaac agatggttta
     3601 aaatcttctt ccttgcttac ttctacgaga gcacttgctg cttccaacat atctgccaca
     3661 acaaagtttg aacctgcaag aactgcttcc ttgatatctt ttggagtaga gcccatcatc
     3721 ttatcaacac gagccttgat aaagtccata acctctgctt tattttcata agtcaaactg
     3781 tcaaatttca atgcataaag gctatcaatc agctcatcca tagcaatgtg ccaaccaaag
     3841 gcatccaaga tacgaaccac accttgagtt gcacgacgaa gggcataagg gtcattagaa
     3901 cctgatggaa tcaatcctac tgagaagaaa ctcaaaatcg tatccaattt gtctgcaatg
     3961 gctagaactg cgccgacctt gctctctgga agttctcctt cagctgatgt aggcatgtag
     4021 tgttcacgaa tagcagctgc caccgctgga gtttcaccag caagaagggt gtatttttca
     4081 cccataattc cttggagttc gtcaaattca ccaaccatac ctgtcaacaa gtcaaacttg
     4141 taaatggctg ctgcacgagc aaggtcaact gtttcatcca ctgacaaacc agctttttct
     4201 gccaaaagta cagtgatttg acccgtacga atcatgtgtt cacgaaggga accaatcttc
     4261 tcatggaagg tgacattgtt taatttttca acaagatctg aaatcaccaa tttttggtct
     4321 tcacgccaga agaattctcc gtcttccaag cgggctacca agactttttc atttcctttg
     4381 atgacatttt tcaaacgctc tgcgtttccg ttacgaacag aaatgaagtt tggcaagagt
     4441 tttccatctt gatcacgaac aacaaagtaa cgctggtgtt ccttcataga agtcaccaag
     4501 acttcttctg gaacttcaag gtatttagca tcaaaacttc ccatgaaggc agttgggtat
     4561 tcaaccaaat tcaagacttc attcagcaaa tccgcatcaa tttcgatacg tacaccatgt
     4621 tttgcctcaa tttccttgat ttggtcaaca atcatttgct cacgttcaca tggatctgcg
     4681 attacaaact gcttacgaag gtcttcttca tagctcaatg ctgactgaat tttggtttct
     4741 ttccccaaga aacgatggcc acggctcaca cgacttccct tgatatcaag gaaatccaag
     4801 tcaaactctt gctcatccaa gagaacagtt aaagtgtgaa cagggcggat gtattcaaag
     4861 ctatttccag cccagtgcat gctgacaggg aaagtcaatg acttcaagac atctacaaca
     4921 ccaggaacaa tggcttcaac tgattggcca acttcttcct tagtgacata gacatattct
     4981 tcacccttaa tttcacggaa ttcgatatct tcaacagtca aacctttccc acggacaaat
     5041 ccttgagctg ctttggtgaa gtttccatca ctatccaagg caattttctt tgctggtccc
     5101 ttgaaatctt ctgtcaaatc agactgtttg tctgataaac cagtcacacg aacagccaaa
     5161 cgacgtggtg ttgagaaggt ttgaatggct tcaaaagaca ggcggttttc cttgaggaag
     5221 gctgccattt tttcgcctag ttgtttttca cttggtgtga caacataggc tggtaattct
     5281 tcaagaccga gttctactaa taagtttttt gtcatgtttt ttctccgaaa agtatctttt
     5341 tattttccag tttgccttat gtgattggca cgcaagcaac caacttcgtt gtttcattgc
     5401 taaaattgga gccaaaagtc tccaattttg cctagtatcc ttagtttcac taaggatacc
     5461 ttcatcactg cggcaactgg ctcagggctc actctgttcg cccattttcg taatttgtca
     5521 ctctctttat tctgcgtctt ctgctaggag tttagctctt gtttcttcat ccaaaagtgg
     5581 gtagcctagg cgtttgcgtt ctgcgacaaa ggttttggct acgacacggg ccaagttacg
     5641 gatacgagcg atatagcctg cacgctctgt tacagatacg gcaccacgcg cgtcaagcag
     5701 attaaaggta tgtgaacatt tgagaacata gtcataggca gggtgtacca agccttcttc
     5761 taatgcacga ccagcttctt tttcaaactt atcaaaattt tcaagcaaca tttcttggtc
     5821 cgaaatttca aatgaatatt ttgagtgctc atactcaggc tggataaaga tttctccgta
     5881 ttttacacca tcagcccact cgatatcata gacagagtct acttcttgaa tgtaagaagc
     5941 caagcgctcc aaaccatagg taacttccgc agtcacaggg ctagttgcca atccaccgac
     6001 ttgttggaaa taagtgaact gagtgatttc cattccgtca agccaaactt cccaaccaag
     6061 accagctgaa ccagttgatg ggttttccca gttgtcctca acaaaacgaa tatcgtgctc
     6121 caaaggattg attcccaatt tttccaaaga ctcaaggtaa agttcttgga tatttgatgg
     6181 agaaggcttc atgaccacct ggaattggtg gtgttggtag agacggttag ggttttcccc
     6241 ataacgaccg tcagcaggac gacgtgatgg ctctacataa gctgcattcc atggctcagg
     6301 tccgatagca cgaaggaaag tgtaaggact cattgttccc gcaccttttt cattgtcata
     6361 agcctgcata agcatacaac cttggtcatt ccaaaatcgt tgcaaagtca aaataatttc
     6421 ttgaaatgtt aatttcttag acattgaata ctccttaata aaaaataatt tcttgcgaca
     6481 cgagtctacc tcgtcgatta tacgaggcaa gacgagttag tactgcgtct agctcagcac
     6541 cctagtgctg agcgtggggc agtccgactg taaggaggtc tctgccactg atgcagtgaa
     6601 atgtcaattt cttagacatt gtttactcct ttagttttta tatttctttt tcaattaatt
     6661 tctgctgaac aaaccaatct aacaagggaa aagttttttc tgtatttgcc cataaatgat
     6721 aagaatctaa gaccgcttgt acactaccta aattttctac caacttatct actgtcaaaa
     6781 agctgcgcca gtattcataa aaaatacttg catagttctt atcataagtc gaagaaccaa
     6841 aatcattcaa tgaatgccaa ccatacttct tctgaaaaag ttctacgaga gattgattac
     6901 aaattttttc cgcttgaaat tcttcttctg tcaagaaata cttgcgacta atatattcaa
     6961 ccatcccctc ttcgaaccag atataagagt cataaccatc aaaatcatct aaaaaatgct
     7021 ccgaccaatg agctaactca tgtcctacaa tctgtaagag gaaattttca gataaagaat
     7081 gatagtgact ttctattgct tgagtttgct gagaacactc gtagtccatc aattgataca
     7141 aatacaactc tttccaaaca gctaaatcag gcgccataac cattcgtcta ttatttgtat
     7201 aggctggaac agcgctttcc ctaataatct gtgtagcagc attaaaatct gaccaaataa
     7261 cggcttgcgg taaatcacag actgcaagtt cattctttaa aaaagattga taatctttca
     7321 acttttcagt atttcttact acaaaatcac gaaatgtagc tagttgacta tcgtctttta
     7381 caagataaag gttgtccacg gctctccttt ctttcacaca atagactgaa taagcaaaag
     7441 aaccacatct gacaatccta ggttgattta acctaggggc gtcagaaacg cggttccacc
     7501 ctaatttatt acttcattgt ttttgaaaat actacagaaa gcgccagccc tttattttgc
     7561 cctacttggc tcccactatc ccaagctcgc ttgaagaacg ccataagact ttctttcctg
     7621 ctgactatta tatcaaaaat tagaataatg tacaattctt tttatgattt tctagaaatc
     7681 cacatcatca actcgtggag cacccgattg aacagcaatc gtttttaagt tttctctctc
     7741 ctcatgactc agttcaattc caaagcaatc aagattagct tgaatacgag aggttgtgac
     7801 agattttgga agtggtaaaa atccttctgc caagctccag gccaaggcta tctgagcaat
     7861 cgattttccg tgatttgctg cgatttcttg cacttgcttg ctatcaaata gttctccctg
     7921 accaaaaggc ccccaagctt ccaataaaat tcccttttca cgacagtaag ctacgacttg
     7981 atcttgatac acacctggcg ccaagcgaac ttgattgacc gcaggaacga tagttgcagt
     8041 ttcaagcaag gcatccaaat gatggggaag aaaattgcta acgccgatag cacggatttt
     8101 cccttcttga tagaggtctt ccatcgctct ccaaacttcc gcattgcgag ttttccatgc
     8161 gtcattttct ctgagcggtt ttgggttcgg ccaatgaatc aaatacaaat ccaaataatc
     8221 caagcccagt ttttctatag atttttccaa agcttgacga gtttgctcat aggtttgctg
     8281 actattccaa agcttggtag ttacgaacat ttcttcacgt ggaactccgc tatctttgat
     8341 tgcttgacca acactttctt cattctgata aatcgccgcc gtatcaatat gacgataacc
     8401 agccttcaag gcttctaaca ctgcacgata ggcttcttct ccatccttag ccttaaaagt
     8461 tccaaatccc aatactggaa tttctactcc attatttaat tgataggtat tcatatgctt
     8521 ctccttctat tttattcatc aattatagca taaaacattt tttaagtagt actatcttct
     8581 gtaaaaaata agtctgtcaa atatctaaca gacctatttt ttcttgctta ttcatcacga
     8641 ctatagtaca gctcatgagc ttttaggcga gtattgagca tctctggtat ggtcacaaag
     8701 gtataacctt gatttttcaa atactcaatg acccttggca aggcattgac tgtcggactg
     8761 tgaatatcat gcatcaaaac gatagagccg ttagctactt gatgctgaat ttctgtcaaa
     8821 atagatgctt catttttact cttccagtcc agactatcca catcccacat gataaagctc
     8881 aaatccaagc tattgcgaat atcatctgta atagcaccat aaggtggacg catgagttta
     8941 gaactagaac ccagcacttt agttagcaca tcctcagtat cagtaatctg ctttttagct
     9001 tcatcaagag agagttgcga gagaatcgga tggctccaac tatggtttcc aacaacatga
     9061 ccttcagatt ttatcctctt caccaagtcc tcattcccag aaacattttt cccaagcaca
     9121 aagaaagtcg ctttaatatc atatttagct agggtctcta atacctgcgg ggtcgttgct
     9181 ggatttggac catcatcaaa ggttagagcg acaacttttt gatatttctt atcaaagtaa
     9241 gattggtaca aggccgcatc tttttcgagt aagtacgaag attggataac atcaaagaaa
     9301 gcagatactg gcaaggctat ctcttctaaa ttttcaacca caggacttgg ataaaggata
     9361 atctgactat ccttgtaatc aaaattccat gcagacaagt cttggtcaga gaagtttttt
     9421 acaatctgct cactctggtc ttgctctatt tttttatcct ctatgaagga ggtcaactct
     9481 tttatcagct gttccttagc cttactagca tctgaaaaca gttggtcaag tgtaaaaggt
     9541 tgcccatctt ctgttaagtg aacctttcct agactggtct tttcagtctc ttcaatctta
     9601 aaagcaacta aatcatagat ttgtttggtc acattacgat taacgactcc ctttaaacct
     9661 gactcttctt gctctgtata gtagaaaaca agattgtcct tactttctag cttgtcctta
     9721 acatcttgat ttatcagctc cctaacagag gaaatcactt tctccccttg gagaggataa
     9781 taggcaatca cttcggcttg cccctgacga aaatgctcct tctgatttcc ctcactcaat
     9841 tgatcatctt tctctttttt gagcgattca atcttttgct caaaactttt ttgctggtag
     9901 atcttaaaag caataaatcc gcctaatagg caaatagaaa ttgctaaaat accaatcaaa
     9961 gccaataata catgtctcgt tttcccgtgt ctgccacgtc ctagtctact tttattcata
    10021 tttatatcat aacaaatttg agcaataatt tcaatgatac aatttggcaa tccacaattc
    10081 ctctagcaag ccaaagcatc ttacatcaac tccaacatcc gtatctgtag agattatgta
    10141 gttttctatc tatggaattt ctagtagatt gaagctagaa tagtagacct atacttctaa
    10201 aacattgtta gaaatcgatt tgactgtcct gatcgatttg tcctgttctc atttcatttt
    10261 actataaaac tatgaaaagc taggtcatct gagaagtaat tactatcgac aattgcgata
    10321 gcgtatactg gtgcgatttg tttgtagctc tggtgggtat caccttcacg ttggcgaatt
    10381 ttttctaggt tttgattaac ctgactgcac agataagccc ataggcgatt gatgaaaaaa
    10441 ttctgatgat gaacctgaat ttcgatgata acttggattc cattatctaa ctccgccaag
    10501 acgtctatac tagtatagaa atcttgcgct gagtagggca tggaaggcaa gacgtgaatg
    10561 ttacttccct ccaaaatcgt cacatttttt gctggcaagt ccagcatatc gcggataaat
    10621 tgacaaatca ttagttgggc tgatacctgg atgtctgaga atcctccttt tcctccttct
    10681 attatagcac atttttaaat ctaggtttgc tagattctat gcttctatct atttattcgg
    10741 aaaagaaagc taatagctta tttgaaattt tctcctctct gttggatagt cccgtctatc
    10801 tatgttataa tgaaagaagg atttaaaatc ggaaaaggag tatttatgct aaaattaggt
    10861 gtcatcggaa ctggcgctat cagccatcat ttcatagaag cagcccatac cagtggagaa
    10921 taccaactgg tcgcaatcta ttctagaaaa ctagaaactg cagcaacctt tgcttctcgc
    10981 tatcagaata tccaactatt cgatcaatta gaagtcttct tcaaatcttc ttttgattta
    11041 gtctatatcg ccagtccaaa ctccttacat tttgctcagg caaaagctgc cttgtctgct
    11101 ggtaaacatg ttattctcga aaagccagct gtcactcaac cacaagaatg gtttgatttg
    11161 attcaaacag ctgaaaaaaa taactgtttt atctttgaag cagctcgtaa ttaccacgag
    11221 aaagccttta ctactatcaa aaacttttta gcagataagc aagtgttagg agcagatttc
    11281 aactatgcca aatactcttc caagatgcct gacttgttgg ctggacaaac accaaatgtc
    11341 ttttcagacc gttttgctgg tggagccctc atggatttgg ggatttatcc cctctatgct
    11401 gctgttcgtc tttttgaaaa agctaatgac gcgacttacc atgctcaaca gcttgacaat
    11461 agcattgacc taaatggaga tggtatcctc ttctacccag actatcaagt tcacatcaag
    11521 gctggaaaaa acatcacttc caatcttcct tgcgagattt atacaacaga tggaaccttg
    11581 actctcaaca cgattgagca tattcgttca gctattttta ccgaccacca aggaaatcaa
    11641 gtccagcttc ctatccaaca ggctcctcat acgatgactg aggaagtcgc tgcatttgca
    11701 cacatgatcc agcaaccaga tctgaatctc taccaaactt ggctgtatga tgcaggctct
    11761 gttcatgagc tactatatac catgcgccag actgctggca ttagatttga ggcagaaaaa
    11821 tgaaaaccaa actaccgact gaatggcaag aactgagtga ccaactcggt ttccaagaat
    11881 tcacccccat tcaaactcaa ctatttgagc cccttcttgc tggagaaaac ctcctaggag
    11941 tgagccaaac cggaactggt aagaccctag cttatctcct accaagtctt ctcagactac
    12001 aaaagaaaaa agcccaacaa ctcttgattc tagcaccaaa tacggaactt gctggacaga
    12061 tttttgatgt atgtaaaacg tgggcagaag ctatcggatt gactgctcaa ctcttccttt
    12121 caggttcaag tcagaaacga caaattgaac gtcttaaaaa ggggccagaa attctgattg
    12181 gaactcctgg ccgtatcttt gaactcatta aacttaaaaa aatcaagatg atgaatgtgg
    12241 aaaccatcat cctggatgaa tttgaccaat tactagatga ttctcagatt cactttgtag
    12301 aaaaaatcac tcactacgca cctcgtgacc accaactggt ctacatgagt gccacgacca
    12361 agtttgatca agaaaagatt gtaccgaata cacgcactat tgatctctct gatcaaaaat
    12421 tggacaatat ccagcatttc tacatgcagg tagaccaacg tcatcgagtg gatatactac
    12481 gaaaactggc tcatgtagag gatttccgtg gtctagtctt cttcaatagc ctgtcagacc
    12541 ttgggaatgc cgaggaaaaa ctacagtatc gtgatatatt ggctgtttcc ctcgctagtg
    12601 atgttaatgt taaatttaga aaagtcatct tagaaaagtt taaagataac caactaaccc
    12661 tgcttcttgc aactgacctt ttggctcgtg gaattgatat cgatagccta gaatgtgtcg
    12721 taaactttga tattcctaga gatagcgaaa cctacacaca ccgtgctggc cgtactggcc
    12781 gtatgggcaa ggaaggatat gttattactc tcgtcactca tccagaagaa atcaaaaaac
    12841 ttaaaaagtt tgcaagtatc cgagaaattg tcctaaaaaa ccaagaactc tatatcaaat
    12901 aaagccccat tgctctatac acatccagtg tatggagctt ttttacaagc aattcaattt
    12961 tatactcatc tgttttcaaa aagcattcta atccatctcc gattaacgat ggactttatc
    13021 acctccttct ccagttcttg tataatatct tgaagttgat tcatgacatc ttccaaagtt
    13081 cgaaaggctt tattcttaaa tccacgttta cgaatctctt tccacacttg ttcaacgggg
    13141 tttatctctg gtgtgtatgg aggaataaat gcaaagccaa tattagtcgg aatttttaag
    13201 gtacttgatt tatgccatat agcattgtcc ataacgagta aaagataatc atctggataa
    13261 gcttgtgaaa gctcttctaa aaaggcgttc atccactcag tattacatcc accagctctt
    13321 aagaaaaatg attcgcctgt atgggcatca acagctccat aacaatagcg aaattctcgt
    13381 atatagtgac tatggacatg tggacctact cctattggag accaacaaga tcccagttta
    13441 ctgattctac cgaaaccagc ctcatcttgg tacatcgagc gaaccttatg aaaacgtcta
    13501 ctggttttaa agcgctttct tttcttcttg aattgagatt ttatttttag acgcgacaat
    13561 ggtttgagcg tctgctttct taggatgttc tggacgtggc ataatatttc gccaaccatg
    13621 gcgcttcaac agttgataga aggcatcacg tgtgtaggaa cgatataagc ctgaaataag
    13681 gtattgtaac aaattctcct gcctctgtag ccttcaaatg gcgggcaaga aaggctttct
    13741 cttcctcaac tgtcatatat gcatggttac gaccaccacg tgtttct
//
LOCUS       DAVKWZ010000051        13328 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000051 DAVKWZ010000000
VERSION     DAVKWZ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13328
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.051"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            173..355
                     /locus_tag="VW854_001637"
     CDS             173..355
                     /locus_tag="VW854_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV0262847.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDE
                     KNIDTYQRVNRLPAKL"
     gene            300..1149
                     /locus_tag="VW854_001638"
     CDS             join(300..713,715..1149)
                     /locus_tag="VW854_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEV0262848.1"
                     /translation="MKKTLTPTKESIDSLRNYEASKQLTDARFKRLVGVQRTTFEEML
                     AVLKTTYQLKHAKGGRKPKLSLEDLLMATLQYVREYRTYEEIAADFGIHESNLIRRSQ
                     WVEATLVQSGVTISRTPLSSEDTVMIDATEVKINRPKKELANDSGKKKCHAMKAQAIV
                     TSQGRIVSLDITVNYCHDMKLFKMSRRNIGQAGKILADSGYQGPMKIYPQAQTPRKSS
                     KLKPLIAEDKAYNHALSKERSKVENIFAKVKTFKIFSTTYRNHRKRFGLRMNLIAGII
                     NHELGF"
     gene            complement(1221..1583)
                     /locus_tag="VW854_001639"
     CDS             complement(1221..1583)
                     /locus_tag="VW854_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262849.1"
                     /translation="MPKTKTLAELADVILWSFDFANDHAHTFFMDNVEWSHADSYFLS
                     FVSDDVEERYTENVYLDSLSVKQKFKFIFDFGDEWRFECQVLREIETEDEEAYLVRSV
                     GTSPEQYPDYDGFDYEEW"
     gene            complement(1961..3841)
                     /locus_tag="VW854_001640"
     CDS             complement(1961..3841)
                     /locus_tag="VW854_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV0262850.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIQGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVENILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDGLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDIL
                     CPILDQHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEFYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(3835..4704)
                     /locus_tag="VW854_001641"
     CDS             complement(3835..4704)
                     /locus_tag="VW854_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV0262851.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLTWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNALCQEAIERMNRNLTQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVEDFVDAYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(4788..7433)
                     /locus_tag="VW854_001642"
     CDS             complement(4788..7433)
                     /locus_tag="VW854_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEV0262852.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVATFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGEQE
                     HREGIYQNGVIDTPFVTVSMDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTACYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFAIECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLAPQEIRTEFIKKEEI"
     gene            complement(7524..8804)
                     /locus_tag="VW854_001643"
     CDS             complement(7524..8804)
                     /locus_tag="VW854_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEV0262853.1"
                     /translation="MVYSKEIVREWLDEVAERAKDYPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDEIF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIKNYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            8976..11060
                     /locus_tag="VW854_001644"
     CDS             8976..11060
                     /locus_tag="VW854_001644"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEV0262854.1"
                     /translation="MKPLLETIDTRFGTTNKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDRDK
                     ASFQPHYLKIFSLRYQIETQLTPTCYGASIRLKQKQGKALSLYLHATDELTVEQVDKR
                     TLALQQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKTSAQADWENLLHRFDIIETGEADRTFFDHCLYRLFLFPQTF
                     YEVDESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLKTASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIANCAKKLKNIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGISLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            complement(11100..12779)
                     /locus_tag="VW854_001645"
     CDS             complement(11100..12779)
                     /locus_tag="VW854_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEV0262855.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGDCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWANDADLPIQLELDLGSPKTFDVIELREDLKLGQRIS
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGPLVEAQKVRVMITEAQSMPLLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIQVLQFQAGESKKDLHLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
BASE COUNT         3742 a         3145 c         2685 g         3756 t
ORIGIN      
        1 gtcaaggaaa attaacccac tttaatttct tttttaaccc gggttaaaaa agaaattaaa
       61 gtgggttaaa aaagaaatta actgagtaaa aactctcgaa attcaattcg ctatattatc
      121 actcaacttg aacaaatacc agaaccacaa aaaacagatg taaaggaaaa atatggactt
      181 aaaatttgaa ggggttgatt tagaatacaa aaaagctaaa aacaatcttc cagagtcttt
      241 ctgggaaacc tactcagcat ttgcaaatac aaatggcggg aaaataattc tcgggataga
      301 tgaaaaaaac attgacacct accaaagagt caatagactc cctgcgaaat tatgaagcaa
      361 gtaaacaact aaccgatgca cgatttaagc gtcttgttgg tgttcagcgc accacttttg
      421 aagagatgtt agctgtgtta aaaacaactt atcaacttaa acacgcaaaa ggtggacgaa
      481 aacctaaatt aagcctagaa gaccttctta tggccactct tcaatatgtg cgagaatacc
      541 gcacttatga agaaattgcg gctgattttg gtattcacga aagcaactta atccgtcgga
      601 gccaatgggt tgaagcaact cttgttcaaa gtggtgttac gatttcaaga actcctctca
      661 gttctgagga cacggtaatg attgatgcga cggaagtaaa aatcaatcgc cctaaaaaaa
      721 gaactagcga atgattctgg taaaaagaaa tgccacgcta tgaaggctca agcgattgtc
      781 acaagtcaag ggagaattgt ttctttggat atcactgtga actattgtca tgatatgaag
      841 ttgttcaaaa tgagtcgcag aaatatcgga caagctggta aaatcttggc tgacagtggt
      901 tatcaagggc ccatgaagat atatcctcaa gcacaaactc cacgtaaatc cagcaaactc
      961 aagccgctaa tagctgaaga taaagcctat aaccatgcgc tatctaagga gagaagcaag
     1021 gttgagaaca tctttgccaa agtaaaaacg tttaaaatat tttcaacaac ctatcgaaat
     1081 catcgtaaac gcttcggatt acgaatgaat ttgattgctg gtattatcaa tcatgaacta
     1141 ggattctagt tttgcaggaa gtctattgaa atactaagcc tacacacaga ctgattccaa
     1201 ttgtttttta gatttcaatt ttaccattct tcatagtcaa aaccatcata atctggatat
     1261 tgttctggcg acgttccaac cgaacgtacg agataagctt cttcgtcctc tgtctcgatt
     1321 tctctcagca cttggcattc aaaacgccat tcatcaccga agtcgaaaat aaacttaaat
     1381 ttttgtttga cacttaggct atccagatag acattttctg tgtaacgttc ttcaacatcg
     1441 tcactaacaa agctaagaaa gtaagaatct gcatgactcc actcaacatt atccatgaaa
     1501 aatgtgtgag catgatcatt tgcaaaatca aaactccata aaatcacatc tgctagttcc
     1561 gctaacgttt ttgtttttgg aatctatata tgtcggtagc aacctttttt aaatttgacg
     1621 gaaatgatcc aagcttctac agatgctggc ttttcagctt ctatttcttg ttttactaat
     1681 tttaagtgta gcataatacc tccaattgct catcgataga attgctctta ggaaacattc
     1741 actttctgct attttatcat aagtttgtct aactgtagca tttgaaacca gataattctt
     1801 ctgtttcacg cgaaacaccc tgcgccaagc aaggtgttct tgtttttata ctcaatgaaa
     1861 atcaaagagc aaactaggaa gctagctgca ggctgctcaa aacactgttt tgaggttgta
     1921 gatggaagct gacgtggttt gaagagattt tcgaagagta ttaagtcgta taaatcgtcg
     1981 acgctgtcgc aatggtatgc cactggttgg ctgtagttgc tgcgaaatcc ttgtctgcgt
     2041 agaagtcagt aaatggtagg atttcaactt ccagctcgtc gatgcggtca agctgaccag
     2101 ccagataaaa ctcgatacgg ctttctgctc gtttgatgcg ttgcaagagt ccgcccatac
     2161 ggatgtcaac tgtatccaaa ccaaagacct tgttttcttt cagccattgg tgactaaaga
     2221 gggcatggaa gtcttcaatt tggcttctaa gttctggtaa ttcttgtctg gcgatttgtt
     2281 gtaaactttc tttatcatcc gcttggtagg cctgacgaat gcgtcgtccc acatctactt
     2341 tgctacttaa aatagcattc aactgggcct gagtttcaaa gagataggca tagtttccag
     2401 ctttttcttt aatgttagca agcgtctcag cagcctgagc gaagtgcggt ttgtcctgtt
     2461 caggtgtcat gtgttgatca agaatcggac aaagaatatc ctgataaaaa acatagcggt
     2521 tgggattgat accgctgaga ttgcctggta ggtctggtaa gaggttggca aggtcaatct
     2581 gcataaaatc ctcaaccgtt agaccagtat tggtcttgaa gtgcgcagac aaaccatcta
     2641 ggtcattgcg atagctgagt tctgcccaga tttgcaagct tggtaggata gagaactggg
     2701 cagtttcacc accattgtct ccccaacccg ttacgatgac ttctttaatc tgattggcac
     2761 ggcaggcttt attagcctcg atagccacta gacggctaaa atggttgtga ggtgtaaagc
     2821 caatccactt ccaagctccc cctgcaaatg caaggtcatg gctaatcttg tgatgattgc
     2881 ggaaattacg gttgtatttt tcctcgctat cctgataata atcccagtaa accagagtca
     2941 cacggtcttt gagacggtct aggtagacac gagtttcctc tggaatttcc acatcacgat
     3001 cgtactggcc atccgctgac atgagtttga agaacatatc actccacatc tggcagtgga
     3061 aaccatattt gtcagcaata tccagcacgc gctccaagtg ttggcacatg aggagactac
     3121 gatccacaac accgttcaaa atcaggtagc gtcccaaacc aaccaagtgg gcttcgtcca
     3181 tcccgatatt gaccttgcga gtcttcagtt tagacaacgt ggcaaacatg ccatcaatca
     3241 ggtcataaac cttttcttcg ccaatgagaa gaatgttctc tacatcacgg agctcctgca
     3301 cttccttgac accccatttg acaaaggccg acaagtgggc caaggtctgg atgcatggta
     3361 caaaggtcat gtcaaactgt tgagcatagg cttctatttc ctgcaactcc tctgctgaat
     3421 aagctccacg gaaatagcca aagtaaggct gtccttcaat ctggtaagtg tcttccatgt
     3481 aaagctcaaa ggttgagtag cccatgagag ccaagacctc aatcatctgc ttggcagaag
     3541 ccacattcag caccgcattt cgagaacagt caaccatgta agccaaatct tcgtaagctg
     3601 cttgttcctc aatctctact ttatcagctt ctgctagaac tgttaccaac aaggacaagg
     3661 cacgataaag ttggtgaggt ttgcggtagg tcaattgata gtgaccttcc tcaccctgga
     3721 tagagataga ggcttggtca gactgagtga cagccacttc cacatctggt agagaaatgt
     3781 gaccttttaa aacctcaata gcttgcgttt gtttgggact aagtcctgta aatcttacca
     3841 ttgcttttcc tcctgtagcc agttgacaag ggcaccgtag agattggcat ctgcgtgata
     3901 ggtgcaggcc tggataactg gtgcgaccgt gtattcttcg taggcatcga caaagtcttc
     3961 aacagccttc ttaacacctt ggataaaatc tggattttga ctgatagagc cacccagact
     4021 gatgacatct ggatcgatga gatactggat attgagcaag ccttgcgtca gattgcggtt
     4081 catgcgctca atggcttctt gacaaagagc attaccagct gcggcctctt ggtaaatctt
     4141 gcgaccgtcc caatcagtat gaccagattt ttcaatcacg tatcgtacca tattcccagt
     4201 tgatgctagt tgcgaccagt tattaagttt ttcagcaggg gcaagggttg tcatgtagcc
     4261 aaattctcca cccagaccgt ggcgacctcg atgaagtcta ccattgataa tcatggctcc
     4321 gccaatccct gtcccaatca cgacacaggc tgcattttca agctctggat gagctagtag
     4381 ttcactgagt ccaacgcagt tggcatcatt ttctaaatgg acaggtagct gataagagct
     4441 aagcgcctca taccaagaaa agccatggat atagggcacc gcactgaagc catcaatcac
     4501 acctgtctct tgattgaccg cacctggaac gctcatagca atcccactgt aatcctgttc
     4561 tgacaagcgt tgatctagcc acgttagtaa atcctccaag ttttcaggcg ttgaaatact
     4621 tgtcttatcc agtattttcc catcaggagt cagactggca aacttaatcc cagtccctcc
     4681 gatatcaatc gttgcaatgg tcattgtttt taccataaaa aggggcgaat cagtagttag
     4741 ttcttacctt ttcgcccctc ctttcttttg atgtttactt tttgaaatta aatttcttct
     4801 tttttgatga attctgtacg aatctcttgt ggagccaata gtcctgaatg aactggatat
     4861 ggtcgctcaa gtaagtcaag aatggtttgt tgatgttctg atatgcgcac attttcttga
     4921 ctcatattat agtaacggag gacatagcct tcttcatttt ccgccacttt aaaggctgtt
     4981 ggacaaactt gcggtatgct gagaacagaa tggctcaaga ggctaccagt cgcaaccacg
     5041 cttccttcct gtctagcaag ctgaaggctg gtaaacggtg tctgcaaggc tttggcacga
     5101 cgataggctg agaagcgttc ttgggcttgg tggcattcaa tcgcaaattc gacttcaaac
     5161 tcccgcaagc attgtgcttc tggcgttggg aagtagcccc agtcacctag ctcacctgat
     5221 gcacgcaaaa tggtcacggc aatggtgtta tccccaagga tttcgtattc attcaatccc
     5281 ttattggata cagtcacccc tttttcatcg tcatacagac tgacaaaagc ttgttggtgt
     5341 tgaggatttt caggattttc ccatgaagca gctggtttgt ttggtcgtgt caccacctca
     5401 tagatacttt cagaatcatt acttggacgc gtgttatgag tcttgaccaa gagacggata
     5461 cggtgatcct tggcagtgtt agtaaagcga gtcttgaagc ggatttgtgg attgtcaacg
     5521 aagacagtca actcagtttc cagaggaatg tttgtcaatt cttctgaccg tccagcctca
     5581 cgcttcataa actcgatgat accttgttgc tcttcttcta gcttttcatc cgcgctgaca
     5641 ggcacggtca attcatgttt gagcaagatt ttagcatagc aagctgtgtt ttccaagacc
     5701 tcgtggccct taagctctgc aaagattggc tctgttcctt ttggttggaa atagatatac
     5761 tcgtttccga tgtccccacg gtcttcaaag cggataaagt cttcataggc ttcgtgagtt
     5821 gtcttatcat agactgtgat gttgtcatcc atactcaccg ttacgaatgg tgtatcaatc
     5881 actccgtttt ggtaaatacc ctcacggtgt tcttgttctc cttccagcaa ttggaaggtt
     5941 gtccaagaaa gcggtgctag gtgaactgga atggtcacgc gcacttgtcg agcaatacga
     6001 gcttggcgga acttgtcttt tggtaaatcg tattcaaaat tagctccgag gtcttcgatt
     6061 gtagcctcta caggacgacc atccaagtcc tccacacggt aacttggcaa gataagagca
     6121 gccatctttt tgtagccttc tgttgggtgc aattccttga aatcacaagt cgccacatca
     6181 atcactgtgc tgacagtatc gaccttatca tgcaagcctg tgttaatgac agtaaagaga
     6241 tagtcacttt gagccttatc cgtagcaatt ttacccttcc actcgttgag caaattactt
     6301 ttaacaaagt ttcctacttg gttgaccttg gcaaaacgcg tttccatctc gcggtgaact
     6361 tcgtccacgc tacagccaca gatactatca tgtggcgcat tctgcaaaag tgttttccaa
     6421 gcataggtca actggtcctt gtggttgtgt ccaccagtga taatagtcaa gggttctacc
     6481 acttgctcta ggaggttgct attttcttgg aaggcttgtt ttaggtaaat gcgggatgaa
     6541 gaagtgttgg caagtgtgta ccagccatct gtttcctgac tggtcaactc acctgtaaca
     6601 gttgataagt gttcaggaag cgcaccttct acagcttgaa catattcatc aaaggaacta
     6661 tgaacaaaga ttacatccgg gaagagttca tttgccacac gaatggcttc gctcagattt
     6721 ttctgtacag gctggtggtc acagccgttc atcatcaacc attggttggt cgaagcgtag
     6781 gcacgcacat ctgacaattt ttgtttccag aaggtcaagg cctcatcttt gtcaactgga
     6841 atttcattcc cgttactgta ccagttggca aagagaatac ctaaaacacg actaccatcc
     6901 acaccctgcc agtacatttc tgaaaactgg gatgtaaact gctcatcttc aaggacttgg
     6961 ttgtcaaatc caatcggctt cacaccacga ccaaaggtcg ccacgtgaat gcctgatttt
     7021 tgaagaattt gaggcgcttg tcccatattt ccaaaggtat ctggaaagta gccaatctgg
     7081 gttgatttac cccatttggc agcttcttgt tgaccaatca aggtattgcg gacattggct
     7141 tcactggaga tcaaataatc atcctgcaag atgtaaaagg gaccaatttt aagcttgccc
     7201 tcgtcaatgt agcgttggac cttgtcacga ttttcagggc gaatttgtaa gtagtcgtca
     7261 aggacaatag tttgtccatc caagtggaaa ctcttgaact cagggtcatt ttcaaagaga
     7321 tcaaagagat tgtcaaacaa ttccaccaac tgcatacgat gactttcaaa aggcaagtac
     7381 cactcacggt cccagtgact atgtgagata atatgtacaa caacattttc catgaagtaa
     7441 aacctcattc taaatttaaa ttttaacgtt ttaaatgtaa acttgtgatt ctgataagaa
     7501 tcggttgagc taaagcgagc cccttagcga atatccaagt aatccaagac caactcacag
     7561 aacatcatgt tagcccagga gaaccattca cgagagtaga gggtcggatc atctacatga
     7621 aagctttcgt gcatgacacc tgtaccacca tcgcaggcaa ccagctgatc cagcaagaat
     7681 tttttctctg ccttatctct tgttgtcaag ccttggatag aaagggcgat tggccagata
     7741 tagcgataga aggtatgaga actgccgaga ccgcttgcgt attctccttg gtagaagtat
     7801 ggattttcag agctcaaaat ggtacgacga gtagcttgat acacttcatc atcgaccgaa
     7861 cagtagccca gatagggcgc agctagtaga cttggtacat ttggatcatc catgatgctg
     7921 gcatttccta ggccatccac ttcaaaagcg taaatctttt cgcccttgct gttggtggtg
     7981 taagcgtagt ttttgattcc ttcttggatt tcatcctgaa gacgcttggc atcagcaata
     8041 acactctggc tatcagctag gtttaatgct gcgaagattt cttgcacata acccaagact
     8101 actacagcaa acatatttga cggaatcaag taactatact ggcaacagtc atcactcgga
     8161 cgaaaagctg accaagtcat acctgtcact gcaaagtcag gtccaaagcc atcatttacc
     8221 aaggtgtctt ccttacggtc cgtatctcgg acaaaacgat aaggagagtt cttgtggtct
     8281 tgttccaccg tccacagatg gagaatttcc ttagtcgctg cgacaaaaat ctcatcaaac
     8341 tgactagtct cgccagtctc tttccagagg agataagcca actgcaaagg atagcaaagc
     8401 gaatccacct catacttgcg ctcccagatc cagccgttaa ggtctgtgtg gtcagtctcg
     8461 tggtgccctt tccagttctc ctcaatgttg aaggagttag catagggatc cttgagtacc
     8521 aaggtcatct gacgtttgac caaacctgca atggtctgac gcaggagggc atctctttta
     8581 gctacatgaa ggtagggtct gagttgggct gtcgaatctc gaagccacat ggcaggaata
     8641 tccccagtca agacaaaagt tgaaccatct tctaagattt caaccgtatt gtccaaggta
     8701 tcggtgtagc aacgctcgaa aacatccacc cactctggat agtccttagc ccgctctgct
     8761 acttcatcta gccactctct gacgatttct ttcgaataaa ccattttaaa ttttacctct
     8821 ttcaaacaag tttcatgttc agtatatagc ttttgagaca gaaaatacat acacaaatga
     8881 tagtcatttt tctacaaaat agttattttt gaaactcctt ttctaaaggc cctctttttc
     8941 tgatataatg tagaaaaaca gctaaaggaa agactatgaa accactactt gaaaccatcg
     9001 atacccgctt tggaactacc aacaagcatg ccttttctcg gggaaatacc ctgccataca
     9061 caggcgtgcc ttttgggatg aattactttg tgccccagac cagtgaccag gatggatctt
     9121 ggttttttga tccacatctg cctatctttc aggggattcg attaactcac cagcccagtc
     9181 cttggattgg ggactactct tggctccttc tgacacctgt caccagtcaa ctgggtggag
     9241 acagcctctt tcaccgccaa tcttcctatg atagggataa ggcctctttc caacctcatt
     9301 atctaaagat tttctccctg cgctatcaga ttgaaaccca gctcacaccg acttgctacg
     9361 gtgcttctat tcgtttgaag caaaagcaag gcaaagccct ctccctctat cttcacgcaa
     9421 cagatgaact gacagtagag caagtagata agcgaactct tgccctgcaa caagaaggta
     9481 aaactgaaac taacaaaaat tcgctaacga tgttcactgc cctgcaaatg aacacggata
     9541 ttcttgctat cagccaagaa gctggagact ggcgaattga cttagcaagt agtcaaaccg
     9601 agatgcaact agccacttct ttcatctctc cttctcaagc tctaatcaat ctacctcaag
     9661 aagattttga tagctgtaaa acaagtgccc aagcggattg ggaaaatctc ctccatcgtt
     9721 ttgacattat agagacagga gaggctgacc gaaccttctt tgaccactgc ctctacagac
     9781 tcttcctctt cccacagact ttttatgagg ttgatgagtc agggcaagcc atccacatgg
     9841 atctggctac tggtactgtc aagcctggtg tcctctttag caacaatggt ttctgggata
     9901 ccttccgcac caccttcccc ctctttgccc ttatcatacc ggagcactat caacgctttt
     9961 tagaaggttt cctcaatagc taccgcgata ctggtttcct tccaaaatgg ctggctccag
    10021 atgaacgggg tatgatgcca ggtacacttt tagacggtat tatcgcagat agcgcctgca
    10081 aggacatggc ccccgaccta gaaggagaac tcttccaagc catgctcaaa acagccagca
    10141 aagccgaccc tctcggcatc aatggccgcc acggactagc ccaataccaa gaactaggtt
    10201 acctctctac cgaccaccac gaaagtgtta gccatactct agactatgcc tatagtgact
    10261 tttgtatcgc caactgtgcc aaaaaactaa aaaacataga aatcgctgaa acctacaagg
    10321 ctgcttcaca aaattaccgc cagctatttg acgctgagac aggttacatg cgagcacgag
    10381 acaatcaagg aaactttcac cctgacttct ctccttatag ttgggggcga gactacgctg
    10441 aatgctctgc cattcaagct actttaggcg ttctccacga catccctggc ttaatccaac
    10501 tgatgggtgg aaaagaaacc tttagcaact atcttttgaa agcctgtcaa gatgctcccc
    10561 tctttgaaac aacaggctat ggttacgaaa ttcacgaaat gagcgagatg gctactgctc
    10621 cttttggaca gatagccatt tccaaccagc ctagtttcca cattccttat ctcttccgtt
    10681 acagcgatta ccctgactac actgcccttc ttatcaagac gctccgtcag aaagcttttc
    10741 acccaagttg ggaagcctat cctggagatg aagacaatgg tagtctctct gcttggtaca
    10801 tctggtcagc tctcggattt tatccaacct gtccaggaaa accaagctat gacctcggaa
    10861 tctctctctt tgaccatcta cgagtctacc tcgctaaaga agataaatgg ctggatatcc
    10921 atactaaaca aaaccacaac cattttaact ttgtcaaaga atgccgactg gacaaaacac
    10981 tcgtatcaac tattcaacac caagacctct taaaagctga acaactaact ttcaccctca
    11041 gctggttacc aagtcattaa taaccaaaag aacctttgca tcgcgcaaag gttcttcttt
    11101 tatgaaactt ggacttgaag ttgatccaca agctgaccat ccaccgttag gttcagatag
    11161 aaatccaagg ttttatctcc tgcgaaatac agggttggta gatgcaaatc ttttttgctt
    11221 tcccctgctt ggaattgaag aacttgaatc tcatcctgat aggcgacacc atggacacct
    11281 gtccccggtt gaatcgaaat cttagcctct aaagaggagc tactttcact acgttcaatc
    11341 ctaaaatgaa gagtctctcc ctttgccaca gctaggcttt tttctgcaaa tgctagttcc
    11401 tgaacaactt ctttttttga taatccagga gttttataaa gggaaatctt ggtcagtaga
    11461 ggcatagatt gtgcttcagt aatcatcacg cgcaccttct gtgcctctac tagcggacct
    11521 cgcaagaggc gcttatgacc aactgtgaag cccctgccaa attcctgcca gacaccatcc
    11581 acctctactt gcacatggaa agcagaaata cgttggccta acttcaaatc ttctctcaac
    11641 tcaattacat caaaagtttt aggagaacct aagtcgagtt ctaactggat gggcaagtct
    11701 gcatcgtttg cccaagagct ggtctcaagg ccgtctgtca aatggcgaca agcaaagtct
    11761 gcggaaagag ctggaccaga tacctcagct cccagagcca aatcttcttt atagagctca
    11821 ttgcgatagg tcgcaaattc ataaagtcgt tcaatatcct ttgcatcaaa gagcccagct
    11881 tgattcggcg gaatattaag caagagtgga gttcctcgcc ctactgagtg aaagtagatt
    11941 tcgaccaact cctcgagaga cttaggatcc tgatcctcat ggtagaacca acctggacgg
    12001 atggaaacat ctgcctctcc gattgaaaaa atcgtgcccg agggatcccc gtgctgaaga
    12061 tagttcagct ctgcttctgt tcctagttta tcaggattca ccttttgcca cagtggatca
    12121 cctgcatacc ctcgttcatt gccaatccag cggatactgg tgccttctgt tgaaaaaatc
    12181 aagcaatcgc cctgcaggtc acgaatggtt tcaaaccatt tttcaaattc ataattaacc
    12241 ttttgcgcgc cctctcctct ggcaccatcc atccaaacct cagcgaactt accagcattc
    12301 ccatagttag gatttgataa gatttccttc aactgagcca gataataggc attgtagtcc
    12361 gcttctcggt ccacatgata gaggggacta tgggcatccc acggtgacag gtagaccccc
    12421 atatccatat caaactctgt ggcagcttgg gatacttcaa ggagcaagtc gccctttcct
    12481 ctcctccaag gactgacctt aaccgaataa tctgtgtgag ctgtcggata aaggacaaag
    12541 ccatcgtggt gcttgaccac caaaatcaac tttttgaagc ccgtttcctt gagcacacga
    12601 acccactcac gcgcatccaa ctgactcggg ttaaagcgct caggatcctc ctgtccagtc
    12661 ccccattctt ggtcataaaa ggtattagga ccgaagtgga taaaagctgc tagttcatct
    12721 cccagataag ctagctgagt ctgacttggt aacggtccat gcggtttgat tttcttcatc
    12781 gttatatatc tccttctttt gcaacttctc ttgttcacaa ggttatccac aggttgtgga
    12841 taataaacca tgatctaaca gccagctctt tccaactcca actggtaggc tcaggatttt
    12901 tctcctcaat gttccctctt caaacagctc tgtcatatgg ttctgctaac acctctcaga
    12961 atgctactct ccataattat tctcaggctt ctttcaggtc attgtgtgaa gaccattcac
    13021 tctccagact tttccacaga ttgtgaataa gtgattattc taagctatca cctcgtcgac
    13081 tataacaaaa aataaaaaga gggtcaataa gataattatc tcttcctaaa tactcagaaa
    13141 agattctata atttgtacaa ttgaacaaga aatgcttctc caaaatctat cttcaaaaga
    13201 atgttgcatc tttttatcct ttcacacaaa catttctctt tatacagatc caagtgataa
    13261 ccagatgata attggacact ttttggtcat ttgtatattt tttgaaaaat tttttaaaaa
    13321 aattttat
//
LOCUS       DAVKWZ010000052        12979 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000052 DAVKWZ010000000
VERSION     DAVKWZ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12979
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.052"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            <1..90
                     /locus_tag="VW854_001646"
     CDS             <1..90
                     /locus_tag="VW854_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEV0262856.1"
                     /translation="FSTTYRNHRKRFGLRMNLSAGIINHELGF"
     gene            281..2572
                     /gene="pcrA"
                     /locus_tag="VW854_001647"
     CDS             281..2572
                     /gene="pcrA"
                     /locus_tag="VW854_001647"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEV0262857.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDSDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKPASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            2615..3295
                     /gene="radC"
                     /locus_tag="VW854_001648"
     CDS             2615..3295
                     /gene="radC"
                     /locus_tag="VW854_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEV0262858.1"
                     /translation="MYSISFQEDSLLPREKLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(3292..3981)
                     /locus_tag="VW854_001649"
     CDS             complement(3292..3981)
                     /locus_tag="VW854_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV0262859.1"
                     /translation="MKKPVIGITGNEKTHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEIACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(3999..4640)
                     /locus_tag="VW854_001650"
     CDS             complement(3999..4640)
                     /locus_tag="VW854_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEV0262860.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(4723..4938)
                     /locus_tag="VW854_001651"
     CDS             complement(4723..4938)
                     /locus_tag="VW854_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV0262861.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGKKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(4946..5293)
                     /locus_tag="VW854_001652"
     CDS             complement(4946..5293)
                     /locus_tag="VW854_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEV0262862.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(5298..6413)
                     /locus_tag="VW854_001653"
     CDS             complement(5298..6413)
                     /locus_tag="VW854_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV0262863.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLKAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(6423..7382)
                     /locus_tag="VW854_001654"
     CDS             complement(6423..7382)
                     /locus_tag="VW854_001654"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV0262864.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(7495..8064)
                     /locus_tag="VW854_001655"
     CDS             complement(7495..8064)
                     /locus_tag="VW854_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEV0262865.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDMTDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            8192..8863
                     /locus_tag="VW854_001656"
     CDS             8192..8863
                     /locus_tag="VW854_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEV0262866.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            8847..9665
                     /locus_tag="VW854_001657"
     CDS             8847..9665
                     /locus_tag="VW854_001657"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEV0262867.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            9662..10558
                     /locus_tag="VW854_001658"
     CDS             9662..10558
                     /locus_tag="VW854_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV0262868.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIYIPAEEGFETLEAIEHPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            10602..11576
                     /gene="pta"
                     /locus_tag="VW854_001659"
     CDS             10602..11576
                     /gene="pta"
                     /locus_tag="VW854_001659"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEV0262869.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(11838..12979)
                     /locus_tag="VW854_001660"
                     /pseudo
     CDS             complement(11838..12979)
                     /locus_tag="VW854_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3799 a         2461 c         2794 g         3925 t
ORIGIN      
        1 ttttcaacaa cctatcgaaa tcatcgtaaa cgcttcggat tacgaatgaa tttgagtgct
       61 ggtattatca atcatgaact aggattctag ttttgcagga agtctattga ggtattgagc
      121 tagtttatga aaaaattggg tgaaaagtcg agtgttttag aaacccacag tgtagtattc
      181 tagtttcaat ccactatatt ttgctactcc ccgtaaagtt tctattttcc ctgatttctg
      241 atataataga aatattgact tcaagagtaa ggaagagaag atgaacgcat tattaaatgg
      301 aatgaatgac cgtcaggctg aggcggtgca aacgacagaa ggtcccttgc taatcatggc
      361 aggggctggt tctggaaaga ctcgtgtttt gacccaccgt atcgcttatt tgattgatga
      421 aaagctggtc aatccttgga atatcttggc cattaccttt accaacaagg ctgcgcgtga
      481 gatgaaagag cgtgcttata gcctcaatcc agcgactcag gactgtctga ttgcgacctt
      541 ccactccatg tgtgtgcgta ttttgcgtcg cgatgcggac catattggct acaatcgtaa
      601 ttttacaatt gtggatcctg gtgaacagcg aacgctcatg aaacgtattc tcaaacagtt
      661 gaacttggac cctaaaaaat ggaatgaacg aactattttg gggaccattt ccaatgctaa
      721 gaatgatttg attgatgatg ttgcttatgc tgcccaagct ggcgatatgt atacgcaaat
      781 tgtggcccag tgttatacag cctatcaaaa agaacttcgt cagtctgaat ccgttgactt
      841 tgatgatttg attatgctga ccttgcgtct ctttgatcaa aatcctgatg ttttgaccta
      901 ctaccagcaa aaattccaat acatccacgt tgatgagtac caagatacca accacgctca
      961 gtaccaattg gtcaaactct tggcttcccg ttttaaaaat atctgtgtgg ttggggatgc
     1021 ggaccagtct atctacggtt ggcgtggtgc tgatatgcag aatatcttgg actttgaaaa
     1081 ggattacccc aaagccaagg ttgttttgtt ggaggaaaat taccgctcaa ccaaaaccat
     1141 tctccaagcg gccaacgagg ttattaaaaa taataaaaat cgccgtccta aaaatctctg
     1201 gactcaaaac gctgatgggg agcaaatcgt ttactatcgt gccgatgatg agctggatga
     1261 ggctgtattt gtagccagaa ccatcgatga acttagtcgc agtcaaaact tccttcataa
     1321 ggattttgca gttctctatc ggactaatgc ccagtcccgt acaattgagg aagccctgct
     1381 caagtctaac attccttata ccatggttgg cggaaccaaa ttctacagcc gtaaggaaat
     1441 tcgcgatatt attgcttatc tcaaccttat tgctaatttg agtgacaata ttagttttga
     1501 gcgtattatc aacgagccta aacgtggaat tggtttaggt acagttgaga aaatccgtga
     1561 ttttgcaaat ttgcaaaata tgtctatgct ggatgcttct gctaatatta tgttgtctgg
     1621 tatcaagggt aaggcagccc aatctatctg ggattttgcc aatatgatgc ttgatttgcg
     1681 ggagcagcta gaccacttaa gcattacaga gttggttgag tccgtcctag aaaaaacagg
     1741 ttatgtcgat attcttaacg cccaagcgac tctagaaagc aaggcacggg ttgaaaatat
     1801 cgaagagttt ctttctgtta cgaagaactt tgatgacacc acggatgtga cagaagagga
     1861 aactggtctg gacaaactga gtcgtttctt aaatgacttg gctttgattg ccgacacaga
     1921 ttcaggtagt caggagacat cagaagtgac cttgatgacc ctgcatgctg ccaaaggtct
     1981 cgaatttcca gttgtctttt tgattgggat ggaagaaaat gtctttccac ttagtcgtgc
     2041 gactgaagat tcagatgaat tagaagaaga gcgccgtcta gcctatgtag gtatcacgcg
     2101 tgcagagaaa attctctatc tgaccaatgc caactcacgc ttgctttttg gtcgtaccaa
     2161 ttataaccgt ccgactcgtt ttattaacga aatcagttca gacttgcttg agtatcaagg
     2221 tctggctcgt cctgcaaata caagctttaa ggcatcatat agcagtggta gtatttcctt
     2281 tggtcaaggt atgagtttgg ctcaggctct tcaagaccgt aaacgcggtg ctgccccaaa
     2341 atcaatccag tcaagcggtc ttccatttgg tcaatttaca gctggcgcaa aaccagcatc
     2401 tagcgaggca aattggtcca ttggtgatat tgctctccac aagaaatggg gagagggaac
     2461 cgttctggaa gtttcaggta gcggtgctag gcaggaattg aaaatcaatt tcccagaagt
     2521 aggtttgaaa aaacttttag ccagtgtggc tccaattgag aaaaaaatct aattttccat
     2581 ccttctcacg aataataaag tgaggaggat ttttatgtac agtatttcat tccaagaaga
     2641 ttcactatta ccaagagaaa agctggccaa ggaaggagtt gaagcgctta gtaaccaaga
     2701 gttgctagct attttactca ggacaggaac acgtcaagct agcgtttttg aaattgccca
     2761 aaaagtcttg aacaatcttt caagcctaac ggatttgaaa aaaatgaccc tgcaggaatt
     2821 gcagagtttg tctggtattg ggcgtgttaa ggccatagaa ttacaagcta tgattgaact
     2881 ggggcatcgt attcacaaac acgagactct tgaaatggaa agtattctca gcagtcaaaa
     2941 gttggccaag aagatgcagc aggaattagg ggataaaaaa caagagcacc tggtggcact
     3001 ctatctcaat actcaaaatc aaatcatcca tcagcagacc atttttatcg ggtctgtaac
     3061 tcgtagtatc gctgaaccgc gagagattct tcactatgca atcaagcata tggcgacttc
     3121 tcttatcttg gtccacaatc atccttcagg agcggtagcg cctagccaaa atgatgatca
     3181 tgtcactaaa cttgttaaag aagcctgcga attgatgggg attgttctct tggaccattt
     3241 gattgtctct cattctaatt actttagtta tcgtgaaaag acagatttaa tctaaagttc
     3301 attaacgaca tagtcaaaga gttttttatc tttgggacga ttttcaaaaa gaagttctgg
     3361 atgccattgg acaccgagaa aggcgacatc atccgtactc atgacagcct caatgatacc
     3421 atctttagga tcatgagcca caacttttaa atttggtgct aagtccttga tgctctggtg
     3481 gtggaaggag ttgatatgag agatttctcc atagatttct tggagaacgg tatctggttc
     3541 tgttaccaag cgttgagttg tgtactcaac agaagaatcc tgccaatggt cttcgatatc
     3601 ttggtacaaa gttccaccca tggcaacgtt aaagagttgg gtaccacggc agacagagaa
     3661 aatgggcttt ttctgtttaa tagcttcctt gatgagggcc agttcgaaga tatctctttg
     3721 aaggtgatag tcatcactat caatggtttt gggttcgcca taaaattttg gatcgacatt
     3781 ttgcccacct gtcaagatga gcttgtcaat caaactgata tagtggcagg ctatttcttg
     3841 atcaccaatc ggtaggatga tgggaatccc tccagcatct ttaacgcctt caacaaagcc
     3901 ttttgctgcg tagctcatca tgatgtcatc atctggatga gttttttcgt ttcctgtaat
     3961 cccaataact ggttttttca taaaatgatt ttcgctttct aatcctcttt tcgcatgaag
     4021 tagaggaggg tttggagttc acttgtcaaa tcgacatact gaacgaccac gtcttttggt
     4081 aaatgcagat ggactggtga aaaactgaga attcctttca caccagcatc aaccaagaga
     4141 ttagcaacct cttgtgactt gacgctggga acagttagga tagcagtctt cacatcagca
     4201 tccttgattt tatccttgat ctgagaaatc ccgtaaatgg gaatcccgtc aggagtttgg
     4261 gtaccgactt caggatggtc gtctaggtca aaggccatga taatcttcat cttgttacgt
     4321 tcgtggaagc ggtagtggag aagggcatgg cccatatttc caataccaac cagcatgaca
     4381 ttggtaatag agttgtcatt gagcaaatcg gcaaaaaatg tcattagttt tttgacatca
     4441 tagccaaaac cacgacgacc aagttcacca aaataggaaa aatcacgacg tacggtcgct
     4501 gaatcaatac cgatagcctc tgcaatttgc ttagagttgg cacgttcaat cttttctgca
     4561 tgaaatctct taaaaattcg atagtagaga gagagtcttt ttgctgtagc ttttggaata
     4621 gcaaactgtt tatctttcac aaaatcacaa cctttctatt cttctatttt atagaaacat
     4681 tgtgaaaaaa tcaacaaaaa taagaaaaaa ctaagaaaaa tcttagtttt gatgtaaaaa
     4741 atctgcatga gatagaaaac ggtagaggtc tccgaccagc ccctgataaa cttttttgcc
     4801 cctaaaagtc agagaagtca cataaagtgt atctggtaag gttacacatc ctgacaaagt
     4861 caacatgaga gcctcatgat cctcatactt gagagtacgc tctacatgat agcagtcctt
     4921 ataggtcagt tcaaacattt tggctctatc tttccgattt tgtaaagaca ccacgttcta
     4981 ccaagctatc catgaggaag tagaattttt cctgatgaat atggtggtct tctgatttga
     5041 aaatatcaac tagacgaagg ccaaacttgt cagtgatatt gattttagcc cctgtaagtt
     5101 ccttgttaat gatgattttg agttggaagc cttcaccgct gtttggcact ttttccaaaa
     5161 ggcgagtcag ttcatagtta ccaaccttag tttcaaaaaa ggtgttatct ttgagggtga
     5221 attttttaac agaagggcta agagtgtaat cgtaacgaca attttttaac tgaatgattt
     5281 tttcaaatgc catatggcta acctccgata atttctttta aggtttttgc gagggtttgt
     5341 aggtcttcaa cggtattttg tggcgacaaa ctgatgcgaa gggattcctt caagcgttct
     5401 gaatttgcgc catacatggc tttaagaaca tggctggatt ggacaacgcc tgcagtacag
     5461 gctgagccag tagagattga aattccagct aaatctagcc gaaggagtaa gaggtcattt
     5521 ttctgaccag gaaatccaat attgagaaca taagggagat gatgttttcc tctattcaag
     5581 taatactgaa tgccctccag ctctgccaga aaggcagttt ctagattttg tacatgttga
     5641 aaatgttctt cttgtttttc taggtcttct tttagggctg caaccatgcc tacgatggca
     5701 ggcagatttt cagttcctgc acgttttttc tgttcctgat ctccgccatg tagataggaa
     5761 tcaaagtcca tgctagatgc gtagagaaaa ccgattccct taggaccatg gaatttgtgg
     5821 gcagaagcag tgagaaaatc aatgcccaat tcttctgaat gaattgggat tttaccaata
     5881 gcctgaactg catcaacatg ataggcagca gggtgttgct tgagtatttg gccaatttca
     5941 gcgatgggaa gtaggtttcc tgtctcatta ttggcaaaca tagtagaaac caaaatcgta
     6001 tcgtcacgta aagccttttg aatttgctgg gctgtgattt cttgattttc tggctggata
     6061 atggttgctt caaacccaaa gtgttgaacc aagtaatcaa ttgtttcaag gacagcatgg
     6121 tgctcgatgg cagttgtgat gatatgtttt ccttgttctt ggtgacgaag acagtagcca
     6181 atgatggtag tattattgcc ttcagtccca ccagaagtga aaaagatatg ttgaggtttt
     6241 gtccttagta actgggctag ttcctgacgg gcttctcgca agagtttgcc agcttgacga
     6301 ccatgaccat gaatactaga aggatttccg tgggtttctt gcataacctt ggtcatagct
     6361 gaaatagcaa ctgctgacat aggagtcgtt gcagcattgt ccaaataaat caaagaatca
     6421 ccttatttct ttttattgta ggcaaagagt gggctgactg gttttctttc gtgaatacgg
     6481 acgatagcat caccaattaa ctcactagca gtgatgtagc atacattttt aggagttttt
     6541 tcttttgttg ctactgaatc agtcacaaga atttctttaa tattagtatt gtcaagaagc
     6601 tcagcagctc cctcgacgaa gagaccgtgg ctagaaacag cataaatttc tgtagctcct
     6661 tcacgttcaa cgattttaga agcttcagag aaggtacgtc ctgtatttaa aatatcatca
     6721 atcaagatag ctttcttacc ttcaacatca ccaataatat aaccttcgtt acgagttgca
     6781 tcgtcttgag ggtagtcgat aatggcgata ggagcatcaa gatattcagc caggctacgc
     6841 gcacgtttga cacctgaatt tttagggcta acgacaacaa catctgaacc aagcaatcct
     6901 ttatcgcagt aatgttttgc gaatagggga acagtgaaaa gattatccac tggaatatca
     6961 aagaaacctt gaacctgaac ggcatgcaaa tcaagagtca ggatacgatc aactccagcc
     7021 ttaaccagca tattggcaac tagttttgct gtaagtggct cacgaggaca agcaatgcgg
     7081 tcttgacgtg catagccaaa atatggaagg acaacgttga tactgtgggc acttgcacgc
     7141 acacaagcat cgaccatgat taacaattcc attaggtggt tgttgacagg gaaacttgtt
     7201 gattggatga tgtaaacatc ataaccacgg acactttctt cgatatttac ttggatttct
     7261 ccgtctgaaa attgacgtga tgatagtttt ccaagtggga caccaacagc ttgggcaatt
     7321 ttttgtgcaa tctcttggtt agagttgagt gcgaaaagtt tcatgttttt tctatctgac
     7381 attatagacc gtcctctgta aactttataa atcctagtta tatttacctt acatatatga
     7441 actggggttt gtgtattttt atcttttcta ttttaccaaa aaatggagat tatttcagct
     7501 atttttcata cttttgacaa atcgaaccaa ttttgaagga gctttttggt aggaaatctg
     7561 atttttctct aaaaattgtc ggaaatcctg tttgccttgc tcatgatttt ccacttcaag
     7621 ctccaattcg taatctgtca tatcaaagta tcggctctga tccagtgcca tgagaccaat
     7681 agctgttttc atttcatagc gaagcgttgt tagacaacca agaacctgcc agttcttact
     7741 ttggatacca tgtttcgcca attcatccag tactagccct tgaggaagtt cttccttact
     7801 cagatagttc tcagcatctt ttagttgcaa tttttggttg tattccatgt ttccaacact
     7861 ctgcgggact ttgagtgtca actcagccca gtcttcaaag gttcgaatgc gcatagcgac
     7921 tttcttttct cgcagttcaa aatcaggcgt gtcgatgtag taatttgttt gaagaacagg
     7981 agtgacacct gtgaactggt cttttagacg attgtattca tcttttttca atagtgtttt
     8041 caattcaatt tctaaatgtt tcatttttct tacctttttt tatcgttgaa agcggattta
     8101 tggtataata agcattgtat ttattgtata tgaatctgga gaaaaaatca aagatatttt
     8161 tgacggataa tatgagaaca agggagaata tatgacctta gaatgggaag aatttctaga
     8221 tccttacatt caagctgttg gtgagttaaa gattaaactt cgtggtattc gtaagcaata
     8281 tcgtaagcaa aataagcatt ctccaattga gtttgtgacc ggtcgagtca agccaattga
     8341 gagcatcaaa gaaaaaatgg ctcgtcgtgg cattacttat gcgaccttgg aacacgattt
     8401 gcaggatatt gctggcttac gtgtgatggt tcagtttgta gatgacgtca aggaagtagt
     8461 ggatattttg cacaagcgtc aggatatgcg aatcatacag gagcgagatt acattactca
     8521 tagaaaagca tcaggctatc gttcctatca tgtggtagta gaatatacgg ttgataccat
     8581 caatggagct aagactattt tggcagaaat tcaaattcgt actttggcca tgaatttctg
     8641 ggcaacgata gaacattctc tcaactacaa gtaccaaggg gatttcccag atgagattaa
     8701 gaagcgactg gaaattacag ctagaatcgc ccatcagttg gatgaagaaa tgggtgaaat
     8761 tcgtgatgat atccaagaag cccaggcact ttttgatcct ttgagtagaa aattaaatga
     8821 cggtgtagga aacagtgacg atacagatga agaatacagg taaacgaatt gatctgatag
     8881 ccaatagaaa accgcagagt caaagggttt tgtatgaatt gcgagatcgt ttgaagagaa
     8941 atcagtttat actcaatgat accaatccgg atattgtcat ttccattggc ggggatggta
     9001 tgctcttgtc ggcctttcat aagtacgaaa atcagcttga caaggtccgc tttatcggtc
     9061 ttcatactgg acatttgggc ttctatacag attatcgtga ttttgagttg gacaagctag
     9121 tgactaattt gcagctagat actggggcaa gggtttctta ccctgttctg aatgtgaagg
     9181 tctttcttga aaatggtgaa gttaagattt tcagagcact caacgaagcc agcatccgca
     9241 ggtctgatcg aaccatggtg gcagatattg taataaatgg tgttcccttt gaacgttttc
     9301 gtggagacgg gctaacagtt tcgacaccga ctggtagtac tgcctataac aagtctcttg
     9361 gcggtgctgt tttacaccct accattgaag ctttgcaatt aacggaaatt gccagcctta
     9421 ataatcgtgt ttatcgaaca ctgggctctt ccattattgt gcctaagaag gataagattg
     9481 aacttattcc aacaagaaac gattatcata ctatttcggt tgacaatagc gtttattctt
     9541 tccgtaatat tgagcgtatt gagtatcaaa tcgaccatca taagattcac tttgtcgcga
     9601 ctcctagcca taccagtttc tggaaccgtg ttaaggatgc ctttatcggt gaggtggatg
     9661 aatgaggttt gaatttatcg cagatgaaca tgtcaaggtt aagacctttt taaaaaagca
     9721 cgaggtttct aagggattgc tggccaagat taagtttcga ggtggagcta ttctggtcaa
     9781 taatcaaccg caaaatgcaa cgtatctatt ggacgttgga gactacgtta ccatttacat
     9841 tcccgctgag gaaggctttg aaaccttgga ggctattgag catccattag atattctcta
     9901 tgaggatgac cactttctag tcttgaataa accctatgga gtggcttcta ttcctagtgt
     9961 caatcactct aataccattg ccaattttat caagggttac tatgtcaagc aaaattatga
    10021 aaatcagcag gttcacattg ttaccagact agatagggac acttctggct tgatgctctt
    10081 tgccaagcac ggttatgccc atgcacgatt agacaagcag ttgcagaaga aatctatcga
    10141 gaaacgctac tttgctttgg ttaagggaga tggacatttg gagccagaag gggaaattat
    10201 tgctccgatt gcgcgtgatg aagattccat tattaccaga cgagtggcta aaggcggaaa
    10261 gtatgcccat acttcataca agattgtagc ttcttatgga aatattcact tggtctatat
    10321 tcacctgcac actggtcgaa cccatcaaat ccgagtccat ttttctcata tcggttttcc
    10381 tttgctggga gatgatttgt atggtggtag tctggaagat ggtattcaac gtcaggctct
    10441 gcattgccat tacctatcct tttatcatcc atttttagag caagacttgc agttagaaag
    10501 tcccttgccg gatgatttca gtaaccttat tacccagtta tcaactaata ctctataaaa
    10561 actgtctcag agtataatta ttatcttaaa ggagaaaact catggaagtt tttgaaagtc
    10621 tcaaagccaa ccttgttggt aaaaatgctc gtatcgttct ccctgaaggg gaagagcctc
    10681 gtattcttca agcaacaaaa cgcttagtaa aagaaacaga agtgattcct gttttgcttg
    10741 gaaatcctga aaaaattaaa atttatcttg aaattgaagg aatcatggat ggttatgagg
    10801 tcatcgaccc tcaacattat cctcaatttg aagaaatggt ttctgccttg gtggagcgtc
    10861 gcaagggcaa aatgactgaa gaagatgtac gcaaggtttt ggttgaagat gtcaactact
    10921 ttggtgtgat gttggtttac ttgggcttgg ttgatggaat ggtgtcagga gcgattcact
    10981 caacagcttc aacagttcgc ccagctctac aaatcatcaa aactcgtcca aatgtaactc
    11041 gtacttcagg agccttcctc atggttcgtg gtacggaacg ttacctattt ggagactgtg
    11101 ccattaatat caatccagat gcagaagcct tggctgaaat tgccatcaac tcagcaatca
    11161 cagctaagat gtttggcatc gaacctaaaa ttgccatgtt gagctattct actaaaggtt
    11221 cagggtttgg tgaaagcgtt gataaggtcg ttgaagcaac taaaattgct cacgacttgc
    11281 gtcctgacct tgaaatcgat ggtgagttgc aatttgatgc ggcctttgtt cccgaaactg
    11341 cagctctgaa agctccggga agtacagtag ctggtcaagc aaatgtcttc atcttcccag
    11401 gtatcgaggc aggaaatatc ggttacaaga tggctgaacg cctgggtggc tttgcggctg
    11461 taggacctgt tttgcaaggt ttaaacaagc cagttaatga tctttctcgt ggatgtaatg
    11521 cagatgatgt ttacaagttg accctcatca cagcagctca agcagttcat caatagtgaa
    11581 aactataaag tgatatacta tgctatactg tagttatgaa actatgtacg aaaagcactg
    11641 ccattaattc ctgagaacta aattactgat tggtgtcaaa aaggaaaact tccaagcgat
    11701 gatatcctgt ctatacacga cctatagaaa tctgtaatat acatatccgt aaaacgataa
    11761 attccctttt tgattttaaa tgagtatgaa aagagaattt tttggctctt tgtcaactgt
    11821 agtgggttga agaaaagcta agctcgagaa aggacaaatt tcatcctttc ttttttgata
    11881 ttcagagcga taaaaatccg ttttttgaag ttttcaaagt tccgaaaacc aaaggcattg
    11941 cgcttgataa gtttgatgag attattggtc gcttccagtt tggcgttaga atagtgtagt
    12001 tgaagggcgt tgataatctt ttctttatct ttgaggaagg ttttaaagac agtctgaaaa
    12061 ataggatgaa cctgcttaag attgtcctca ataagtccga aaaatttctc tggttcctta
    12121 ttctggaagt gaaaaagcaa gagttgatag agctgatagt ggtgtttcaa gtcttccgaa
    12181 tagctcaaaa gcttgtttaa aatctcttta ttggttaagt gcatacgaaa aataggacga
    12241 taaaatcact tatcactcag tttacggcta tcctgttgaa tgagtttcca gtagcgcttg
    12301 atagccttgt attcatggga ttttcgatga aactgattca tgatttggac acgcacacga
    12361 ctcatagcac ggctaagatg ttgtacaatg tgaaagcgat caagaacgat tttagcattc
    12421 gggagtgaaa cagcctggga gactgtttca gcctgagcct agaaatttga aagcgaagct
    12481 gtttagccaa gtcatagtaa gggctaaaca tatccatagt aatgattttg acgcgacatc
    12541 ggacggctct atcatattta agaaagtgat ctcggatgac agcttgtgtt ctaccttcaa
    12601 gaacagtgat gatattgaga ttgttaaaat cttgcgcaat aaagctcatc tccatctccc
    12661 gattgaaaca gtcactcccc ggactgtttc aacgtcccaa gacataatct taggcagatt
    12721 tctaaaatta cactcaaagt ggaagtcatt gagctttcga atgacagttg aagttgagat
    12781 ggaaagctga tgggcaatat cagtcataga aatcttttca attaactttt gagcaatctt
    12841 ttggttgatg atacgaggga tttgatgatt cttcttgacg atagaagttt cagcaaccat
    12901 aattttagaa caatgatagc acttgaaacg acgctttcta aggagaattc tagtaggcat
    12961 accagttgtt tcaagataa
//
LOCUS       DAVKWZ010000053        12639 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000053 DAVKWZ010000000
VERSION     DAVKWZ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12639
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.053"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            23..1159
                     /gene="dnaN"
                     /locus_tag="VW854_001661"
     CDS             23..1159
                     /gene="dnaN"
                     /locus_tag="VW854_001661"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEV0262870.1"
                     /translation="MIHFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IEAVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            1224..1418
                     /locus_tag="VW854_001662"
     CDS             1224..1418
                     /locus_tag="VW854_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEV0262871.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            1502..2617
                     /gene="ychF"
                     /locus_tag="VW854_001663"
     CDS             1502..2617
                     /gene="ychF"
                     /locus_tag="VW854_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEV0262872.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            2688..3257
                     /locus_tag="VW854_001664"
     CDS             2688..3257
                     /locus_tag="VW854_001664"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEV0262873.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDEYIGI
                     LQSVDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            3258..6767
                     /gene="mfd"
                     /locus_tag="VW854_001665"
     CDS             3258..6767
                     /gene="mfd"
                     /locus_tag="VW854_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEV0262874.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLMSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERELAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTNICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNIDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGFGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKEFKEENSI"
     gene            6835..7101
                     /locus_tag="VW854_001666"
     CDS             6835..7101
                     /locus_tag="VW854_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEV0262875.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            7094..7462
                     /locus_tag="VW854_001667"
     CDS             7094..7462
                     /locus_tag="VW854_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEV0262876.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            7582..8850
                     /locus_tag="VW854_001668"
     CDS             7582..8850
                     /locus_tag="VW854_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEV0262877.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMAYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKVWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            8847..10124
                     /gene="tilS"
                     /locus_tag="VW854_001669"
     CDS             8847..10124
                     /gene="tilS"
                     /locus_tag="VW854_001669"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEV0262878.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLTLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ESIQQIPVSRETAIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            10128..10670
                     /gene="hpt"
                     /locus_tag="VW854_001670"
     CDS             10128..10670
                     /gene="hpt"
                     /locus_tag="VW854_001670"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEV0262879.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKAREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            10686..>12639
                     /gene="ftsH"
                     /locus_tag="VW854_001671"
     CDS             10686..>12639
                     /gene="ftsH"
                     /locus_tag="VW854_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEV0262880.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMLGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDE"
BASE COUNT         4359 a         1901 c         2569 g         3810 t
ORIGIN      
        1 aaaaataaat aaaggagaat ccatgattca tttttcaatt aataaaaatt tatttctaca
       61 agcattaaat actactaaga gagctattag ttctaaaaat gccattccta ttttatcaac
      121 agtaaaaatt gacgtgacca atgaaggtat tactttaatt ggttcaaatg gtcaaatttc
      181 aattgaaaat tttatttctc aaaaaaatga agatgctggt ttgttaatta cttctttagg
      241 ttcgatcctt cttgaagctt ctttctttat caatgtagta tctagtttac ctgatgtaac
      301 tcttgatttt aaagaaattg aacaaaatca aattgtttta accagtggca aatcagaaat
      361 taccctaaaa ggaaaagata gcgaacaata tccacgaatc caagaaattt cagcaagcac
      421 tcctttaata cttgaaacaa aattactcaa gaaaattatt aatgaaacag cctttgctgc
      481 aagtacacaa gagagtcgtc cgattttaac aggtgtccac ttcgtattga gtcaacacaa
      541 agagttaaaa acagttgcaa cagactctca tcgcctaagc cagaaaaaat tgactcttga
      601 aaaaaatagt gatgattttg atgtcgtaat tcctagccgt tctctacgcg aattttcagc
      661 ggtatttaca gatgatatcg aagctgtaga gattttcttt gccaataacc aaatcctctt
      721 tagaagcgaa aatattagct tctatactcg tctcctagaa ggaaactatc ctgatacaga
      781 tcgcttgatt ccaacagact ttaacactac tattactttt aatgtggtaa acttacgcca
      841 gtcaatggag cgtgcccgtc ttttatcaag tgcgactcaa aatggtactg tgaaacttga
      901 aattaaggat ggggttgtta gcgcccatgt tcactctcca gaagttggta aagtaaacga
      961 agaaatcgat actgatcagg ttactggtga agatttgacc attagtttca acccaactta
     1021 cttgattgat tctcttaaag ctttaaatag cgaaaaggtg accattagct ttatctcagc
     1081 tgttcgtcca tttactcttg tgccagcaga tactgacgaa gacttcatgc agctcattac
     1141 accagttcgt acaaattaag tgaaagaggt tgagcctggc tcgcctcttt tatgatataa
     1201 tcgaaaaaga aaaggagagt agtatgtatc aagttggaaa ttttgttgag atgaaaaaat
     1261 cacacgcttg tacaatcaag tcgactggta aaaaggctaa tcgttgggaa attacacgtg
     1321 taggagcaga tatcaaaata aaatgcagta attgtgagca tgttgtcatg atggggcgat
     1381 atgattttga gcgaaaaatg aataaaatta ttgactgaga acccttagtt agagggttag
     1441 cactttatcc ctttttgtgt tataatatta gggattgaaa tgaaaacgga gaatgagaaa
     1501 tatggctttg acagcaggta tcgttggttt gccaaacgtt ggtaaatcaa cactatttaa
     1561 tgcaattaca aaagcaggag cagaggcagc aaactaccca tttgcgacta ttgatccaaa
     1621 tgttggaatg gtggaagttc cagatgaacg cctacaaaaa ctaactgaaa tgataactcc
     1681 taaaaagaca gttcccacaa catttgaatt tacagatatt gcagggattg taaaaggagc
     1741 ttcaaaagga gaagggctag ggaataaatt cttggccaat attcgtgaag tagatgcgat
     1801 tgttcacgta gttcgtgctt ttgatgatga aaatgtaatg cgcgagcaag gacgtgaaga
     1861 cgcctttgta gatccacttg cagatattga tacaattaat ctggaattaa ttcttgctga
     1921 cttagaatca gtgaacaaac gatatgcgcg tgtagaaaag atggcacgta cgcaaaaaga
     1981 taaagaatca gtagcagaat tcaatgttct tcaaaagatt aaaccagtcc tagaagacgg
     2041 gaaatcagct cgtaccattg aatttacaga tgaggaacaa aaggttgtca aaggtctttt
     2101 ccttttgacg actaaaccag ttctttatgt agctaatgtg gacgaggatg tggtttcaga
     2161 acctgactct atcgactatg tcaaacaaat tcgtgaattt gcagcgacag aaaatgctga
     2221 agtagtcgtt atttctgcgc gtgctgagga agaaatttct gaattggatg atgaagataa
     2281 aaaagagttt cttgaagcca ttggtttgac agaatcaggt gtagataagt tgacgcgtgc
     2341 agcttaccac ttgcttggat tgggaactta cttcacagct ggtgaaaaag aagttcgcgc
     2401 ttggactttc aaacgtggta tgaaggctcc tcaagcagct ggtattatcc actcagactt
     2461 tgaaaaaggc tttattcgtg cagtaaccat gtcatatgaa gatctagtga aatacggatc
     2521 tgaaaaggcc gtaaaagaag ctggacgctt gcgtgaagaa ggaaaagaat atatcgttca
     2581 agatggcgat atcatggaat tccgctttaa tgtctaaaaa ttaataaatg gtgtcaatta
     2641 ggttggaaaa aaattccaac ccttttggct tttgaaagga aaaataaatg accaaattac
     2701 ttgtaggctt gggaaatcca ggggataaat attttgaaac aaaacacaat gttggtttta
     2761 tgttgattga tcaactagcg aagaaacaga atgtcacttt tacacacgat aagatatttc
     2821 aagctgacct agcatccttt ttcctaaatg gagaaaaaat ttatctggtt aaaccaacga
     2881 cctttatgaa tgaaagtgga aaagcagttc atgctttatt aacttactat ggtttggata
     2941 ttgacgattt acttatcatt tacgatgatc ttgacatgga agttgggaaa attcgtttaa
     3001 gagcaaaagg ctcagcaggt ggtcataatg gtatcaagtc tattattcaa catataggaa
     3061 ctcaggtctt taaccgtgtt aagattggaa ttggaagacc taaaaatggt atgtcagttg
     3121 ttcatcatgt tttgagtaag tttgacaggg atgagtatat cggtatttta cagtctgttg
     3181 acaaagttga cgattctgta aactactatt tacaagagaa aaattttgag aaaacaatgc
     3241 agaggtataa cggataaatg gtgaccttat tagatttatt ctcagaaaat gatcagatta
     3301 aaaaatggca tcaaaattta acagataaga aaagacaact aatacttggt ttatcaacat
     3361 ctactaaggc tcttgcaatt gcaagcagtt tagaaaaaga agataggatt gtgttattga
     3421 tgtcaactta tggagaagca gaaggacttg ttagtgatct tatatctatc ttgggtgagg
     3481 aactcgtcta tccatttttg gtagatgatg ctcctatggt ggagtttttg atgtcttcac
     3541 aggaaaaaat tatttcacgg gttgaagcct tgcgtttttt gactgattca tctaagaaag
     3601 ggattttagt ttgtaatatc gcagcaagtc gattgatttt accgtctccc aatgcattca
     3661 aagatagtat tgtaaaaatc tcagttggtg aagaatatga tcaacacgcg tttatccatc
     3721 agttaaagga aaatggctat cgaaaagtta ctcaagtaca aactcagggc gaatttagtc
     3781 ttcgaggaga tattttagat atttttgaaa tatcccagtt agaaccttgt cgaattgagt
     3841 tttttggtga tgaaattgat ggtatcaggt catttgaagt agaaacacaa ttatcgaaag
     3901 aaaataagac agaactcact atctttccag ctagtgatat gcttttgaga gaaaaggatt
     3961 atcaacgagg acagtcagct ttagaaaaac aaatttcaaa aactttatca cctattttga
     4021 aatcatacct agaagaaatt ctttcaagtt ttcaccaaaa acaaagtcat gcagactctc
     4081 ggaagttttt atctttgtgc tatgataaga catggactgt ctttgattat attgaaaaag
     4141 atactccaat attctttgat gattatcaaa aattgatgaa tcagtatgaa gtctttgaaa
     4201 gagaattagc gcagtacttt acagaagaat tacagaatag taaagcattt tctgatatgc
     4261 agtatttttc tgatattgaa caaatctata aaaaacaaag tccagtgacc tttttttcta
     4321 atcttcaaaa gggtttagga aatctcaaat ttgacaaaat ttatcaattc aatcaatatc
     4381 ctatgcagga atttttcaat cagttttctt ttctaaaaga agaaattgaa cgatataaaa
     4441 aaatggatta caccattatt ctgcagtcta gcaattcaat gggaagtaaa acattggagg
     4501 atatgttaga ggaatatcag attaaattgg attctagaga taagacaaat atctgtaaag
     4561 aatctgtaaa cttaatagag ggtaatctca gacatggttt tcattttgta gatgaaaaga
     4621 ttttattgat aactgaacat gagatttttc aaaagaaatt aaagcgtcgt tttcgaagac
     4681 aacatgtttc aaatgcagag agattaaaag attacaatga acttgaaaaa ggggactatg
     4741 ttgtccatca tatccatggg attggtcaat atctaggaat tgaaaccatt gaaatcaagg
     4801 gaattcatcg tgattatgtc agtgtccaat accaaaatgg tgatcaaatt tctattcccg
     4861 tggaacagat tcatctactg tccaaatata tttcaagtga tggtaaagct ccaaaactca
     4921 ataaattaaa tgacggtcat tttaaaaagg ccaagcaaaa ggttaagaac caggtagagg
     4981 atatagctga tgatttaatc aaactctact ctgaacgtag tcagttgaag ggttttgctt
     5041 tctcagctga tgatgatgat caagatgcct ttgatgatgc tttcccttat gttgaaacgg
     5101 atgatcaact tcgtagtatt gaggaaatca agagggatat gcaggcttct cagccaatgg
     5161 atcgactttt agttggggat gttggttttg gaaagactga agttgctatg cgtgcagcct
     5221 ttaaagcagt caatgatcac aaacaggttg tcattctagt tccgacgacg gttttagcgc
     5281 aacagcacta tacgaatttt aaggaacgat tccaaaattt tgcagttaat attgatgtgt
     5341 tgagtcgctt tagaagtaaa aaagagcaga ctgcaacact tgaaaaattg aaaaacggtc
     5401 aagtcgatat tttgattgga acacatcgtg ttttgtcaaa agatgttgtg tttgctgatt
     5461 tgggcttgat gattattgat gaggaacagc gatttggtgt caagcataag gaaactttga
     5521 aagaactgaa gaaacaagtg gatgtcctaa ccttgaccgc tacgccaatc cctcgtaccc
     5581 tccatatgtc tatgctggga atcagagatt tgtctgttat tgaaactccg ccgactaatc
     5641 gctatcctgt tcagacctat gttttggaaa agaatgatag tgtcattcgt gatgctgtct
     5701 tgcgtgaaat ggagcgtgga ggtcaagttt actatcttta caacaaagtt gacacaattg
     5761 ttcagaaggt ttcagaatta caggagttga ttccggaggc ttcgattgga tatgttcatg
     5821 gtcgaatgag tgaagtccag ttggaaaata ctctattaga ctttattgag ggacaatacg
     5881 atatcttggt gacgactact attattgaga caggggtgga cattccaaat gctaatactt
     5941 tatttattga aaatgcggac catatgggct tgtcaacctt atatcagtta agaggaagag
     6001 tcggtcgtag taatcgtatt gcttatgctt atctcatgta tcgtccagaa aaatcaatca
     6061 gtgaagtctc tgaaaagaga ttagaagcga ttaaaggatt tacagaattg ggctttggct
     6121 ttaagattgc aatgcgagat ctttcgattc gtggagcagg aaatctttta ggaaaatccc
     6181 agtctggttt cattgattct gttggttttg aattgtattc gcagttatta gaggaagcta
     6241 ttgctaaacg aaacggtaat gctaacgcta acacaagaac caaagggaat gctgagttga
     6301 ttttgcaaat tgatgcctat cttcctgata cttatatttc tgatcaacga cataagattg
     6361 aaatttacaa gaaaattcgt caaattgaca accgtgtcaa ttatgaagag ttacaagagg
     6421 agttgataga ccgttttgga gaatacccag atgtagtagc ctatctttta gagattggtt
     6481 tggtcaaatc atacttggac aaggtctttg ttcaacgtgt ggaaagaaaa gataataaaa
     6541 ttacaattca atttgaaaaa gtcactcaac gactgttttt agctcaagat tattttaaag
     6601 ctttatccgt aacgaactta aaagcaggca tcgctgagaa taagggatta atggagcttg
     6661 tatttgatgt ccaaaataag aaagattatg aaattttaga aggtctgctg atttttggag
     6721 aaagtttatt agagataaaa gagtttaagg aagaaaattc catttgatat ttttcttcta
     6781 taaaatagat aaaaatagat aaaaatggta caataataaa ttgaggtaat aaggatgaga
     6841 ttagataaat atttaaaagt atcgcgaatt atcaagcgtc gtacagtcgc aaaggaagta
     6901 gcagataaag gtagaatcaa ggttaatgga atcttggcca aaagttcaac ggacttgaaa
     6961 gttaatgacc aagttgaaat tcgctttggc aataagttgc tgcttgtaaa agtactagag
     7021 atgaaagata gtacaaaaaa agaagatgca gcaggaatgt atgaaattat cagtgaaaca
     7081 cgggtagaag aaaatgtcta aaaatattgt acaattgaat aattctttta ttcaaaatga
     7141 ataccaacgt cgtcgctacc tgatgaaaga acgacaaaaa cggaatcgtt ttatgggagg
     7201 ggtattgatt ttgattatgc tattatttat cttgccaact tttaatttag cgcagagtta
     7261 tcagcaatta ctccaaagac gtcagcaatt agcagacttg caaactcagt atcaaacttt
     7321 gagtgatgaa aaggataagg agacagcatt tgctaccaag ttgaaagatg aagattatgc
     7381 tgctaaatat acacgagcga agtactatta ttctaagtcg agggaaaaag tttatacgat
     7441 tcctgacttg cttcaaaggt gataaaatgg aaaatttatt agacgtaata gagcaatttt
     7501 tgagtttgtc agatgaaaag ctggaagaat tggctgataa aaatcaatta ttgcgtttac
     7561 aagaagaaaa ggaaaggaag aatgcgtaaa ttcttaatta ttttgttgct accaagtttt
     7621 ttgaccattt caaaagtcgt tagcacagaa aaagaagtcg tctatacttc gaaagaaatt
     7681 tattaccttt cacaatctga ctttggtatt tattttagag aaaaattaag ttctcccatg
     7741 gcttatggag aggttcctgt ttatgcgaat gaagatttag tagtggaatc tgggaaattg
     7801 actcccaaaa caagttttca aataaccgag tggcgcttaa ataaacaagg aattccagta
     7861 tttaagctat caaatcatca atttatagct gcggacaaac gatttttata tgatcaatca
     7921 gaggtaactc caacaataaa aaaagtatgg ttagaatctg actttaaact gtacaatagt
     7981 ccttatgatt taaaagaagt gaaatcatcc ttatcagctt attcgcaagt atcaatcgac
     8041 aagaccatgt ttgtagaagg aagagaattt ctacatattg atcaggctgg atgggtagct
     8101 aaagaatcaa cttctgaaga agataatcgg atgagtaaag ttcaagaaat gttatctgaa
     8161 aaatatcaga aagattcttt ctctatttat gttaagcaac tgactactgg aaaagaagct
     8221 ggtatcaatc aagatgaaaa gatgtatgca gccagcgttt tgaaactctc ctatctctat
     8281 tatacgcaag aaaaaataaa tgagggtctt tatcagttag atacgactgt aaaatacgta
     8341 tctgcagtca atgattttcc aggttcttat aaaccagagg gaagtggtag tcttcctaaa
     8401 aaagaagata ataaagaata ttctttaaag gatttaatta cgaaagtatc aaaagaatct
     8461 gataatgtag ctcataatct attgggatat tacatttcaa accaatctga tgccacattc
     8521 aaatccaaaa tgtctgccat tatgggagat gattgggatc caaaagaaaa attgatttct
     8581 tctaagatgg ccgggaagtt tatggaagct atttataatc aaaatggatt tgtgctagag
     8641 tctttgacta aaacagattt tgatagtcag cgaattgcca aaggtgtttc tgttaaagta
     8701 gctcataaaa ttggagatgc ggatgaattt aagcatgata cgggtgttgt ctatgcagat
     8761 tctccattta ttctttctat tttcactaag aattctgatt atgatacgat ttctaagata
     8821 gccaaggatg tttatgaggt tctaaaatga gggaaccaga ttttttaaat cattttctca
     8881 agaagggata tttcaaaaag catgctaagg cggttctagc tctttctggt ggattagatt
     8941 ctatgtttct atttaaggta ttgtctactt atcaaaaaga gttagagatt gaattgattc
     9001 tggctcatgt gaatcataag cagagaattg aatcagattg ggaagaaaag gaattaagga
     9061 agttggctgc tgaagcagag cttcctattt atatcagcaa tttttcagga gaattttcag
     9121 aagcgcgtgc acgaaatttt cgttatgatt tttttcaaga ggttatgaaa aagacaggtg
     9181 cgacagcttt agtcactgcc caccatgctg atgatcaggt ggaaacgatt ttgatgcgct
     9241 tgattcgagg aactcgcttg cgctatctat caggaattaa ggagaagcaa gtagtcggag
     9301 agatagaaat cattcgtccc ttcttgcatt ttcagaaaaa agactttcca tcaatttttc
     9361 actttgaaga tacatcaaat caggagaatc attattttcg aaatcgtatt cgaaactctt
     9421 acttaccaga attggaaaaa gaaaatcctc gatttaggga tgcaatctta ggcattggca
     9481 atgaaatttt agattatgat ttggcaatag ctgaattatc taacaatatt aatgtggaag
     9541 atttacagca gttattttct tactctgagt ctacacaaag agttttactt caaacttatc
     9601 tgaatcgttt tccagatttg actcttacaa aagctcagtt tgctgaagtt cagcagattt
     9661 taaaatctaa aagccagtat cgtcatccga ttaaaaatgg ctatgaattg ataaaagagt
     9721 accaacagtt tcagatttgt aaaatcagtc cgcaggctga tgaaaaggaa gatgaacttg
     9781 tgttacacta tcaaaatcag gtagcttatc aaggatattt attttccttt ggacttccat
     9841 tagaaggtga atcaattcaa caaatacctg tttcacgtga aacagccata cacattcgtc
     9901 atcgaaaaac aggagatgtt ttgattcaaa atgggcatag aaaaaaactc agacgtttat
     9961 ttattgattt gaaaatccct atggaaaaga gaaactctgc tcttattatt gagcaatttg
    10021 gtgaaattgt ctcaattttg ggaattgcga ccaataattt gagtaaaaaa acgaaaaatg
    10081 atataatgaa cactgtactt tatatagaaa aaatagatag gtaaaaaatg ttagaaaacg
    10141 atattaaaaa agtcctcgtt tcacacgatg aaattacaga agcagctaaa aaactaggtg
    10201 ctcaattaac taaagattat gcaggaaaaa atccaatctt agttgggatt ttaaaaggat
    10261 ctattccttt tatggctgaa ttggtcaaac atattgatac acatattgaa atggacttca
    10321 tgatggtttc tagctaccat ggtggaacag caagtagtgg tgttatcaat attaaacaag
    10381 atgtgactca agatatcaaa ggaagacatg ttctatttgt agaagatatc attgatacag
    10441 gtcaaacttt gaagaatttg cgagatatgt ttaaagcaag agaagcagct tctgttaaaa
    10501 ttgcaacctt gttggataaa ccagaaggac gtgttgtaga aattgaggca gactatacct
    10561 gctttactat cccaaatgag tttgtagtag gttatggttt agactacaaa gaaaattatc
    10621 gtaatcttcc ttatattgga gtattgaaag aggaagtgta ttcaaattag aaagaataat
    10681 ctttaatgaa aaaacaaaat aatggtttaa ttaaaaatcc ttttctatgg ttattattta
    10741 tctttttcct tgtgacagga ttccagtatt tctattctgg gaataactca ggaggaagtc
    10801 agcaaatcaa ctatactgag ttggtacaag aaattaccga tggtaatgta aaagaattaa
    10861 cttaccaacc aaatggtagt gttatcgaag tttctggtgt ctataaaaat cctaaaacaa
    10921 gtaaagaagg aacaggtatt cagtttttca cgccatctgt tactaaggta gagaaattta
    10981 ccagcactat tcttcctgca gatactaccg tatcagaatt gcaaaaactt gctactgacc
    11041 ataaagcaga agtaactgtt aagcatgaaa gttcaagtgg tatatggatt aatctactcg
    11101 tatccattgt gccatttgga attctattct tcttcctatt ctctatgatg ggaaatatgg
    11161 gaggaggcaa tggccgtaat ccaatgagtt ttggacgtag taaggctaaa gcagcaaata
    11221 aagaagatat taaagtaaga ttttcagatg ttgctggagc tgaggaagaa aaacaagaac
    11281 tagttgaagt tgttgagttc ttaaaagatc caaaacgatt cacaaaactt ggagcccgta
    11341 ttccagcagg tgttcttttg gagggacctc cggggacagg taagactttg cttgctaagg
    11401 cagtcgctgg agaagcaggt gttccattct ttagtatctc aggttctgac tttgtagaaa
    11461 tgtttgtcgg agttggagct agtcgtgttc gctctctttt tgaggatgcc aaaaaagcag
    11521 caccagctat catctttatc gatgaaattg atgctgttgg acgtcaacgt ggagtcggtc
    11581 tcggcggagg taatgacgaa cgtgaacaaa ccttgaacca acttttgatt gagatggatg
    11641 gttttgaggg aaatgaaggg attatcgtca tcgctgcgac aaaccgttca gatgtacttg
    11701 atcctgccct tttgcgtcca ggacgttttg atagaaaagt attggttggc cgtcctgatg
    11761 ttaaaggtcg tgaagcaatc ttgaaagttc acgctaagaa caagccttta gcagaagatg
    11821 ttgatttgaa attagtggct caacaaactc caggctttgt tggtgctgat ttagaaaatg
    11881 tcttgaatga agcagcttta gttgctgctc gtcgcaataa atcgataatt gatgcttcag
    11941 atattgatga agcagaagat agagttattg ctggaccttc taagaaagat aagacagttt
    12001 cacaaaaaga acgagaattg gttgcttacc atgaggcagg acataccatt gttggtctag
    12061 tcttgtcgaa tgctcgcgtt gtccataagg ttacaattgt accacgcggc cgtgcaggcg
    12121 gatacatgat tgcacttcct aaagaggatc aaatgcttct atctaaagaa gatatgaaag
    12181 agcaattggc tggcttaatg ggtggacgtg tagctgaaga aattatcttt aatgtccaaa
    12241 ctacaggagc ttcaaacgac tttgaacaag cgacacaaat ggcacgtgca atggttacag
    12301 agtacggtat gagtgaaaaa cttggcccag tacaatatga aggaaaccat gctatgcttg
    12361 gtgcacagag tcctcaaaaa tcaatttcag aacaaacagc ttatgaaatt gatgaagagg
    12421 ttcgttcatt attaaatgag gcacgaaata aagctgctga aattattcag tcaaatcgtg
    12481 aaactcacaa gttaattgca gaagcattat tgaaatacga aacattggat agtacacaaa
    12541 ttaaagctct ttacgaaaca ggaaagatgc ctgaagcagt agaagaggaa tctcatgcac
    12601 tatcctatga tgaagtaaag tcaaaaatga atgacgaaa
//
LOCUS       DAVKWZ010000054        12433 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000054 DAVKWZ010000000
VERSION     DAVKWZ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12433
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.054"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            120..3221
                     /locus_tag="VW854_001672"
     CDS             120..3221
                     /locus_tag="VW854_001672"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEV0262881.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGVK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQECFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQIMLNSANSDYLIDALEAGFEVAPLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSLHHFVAKSNELEEKLNEIVMKT
                     IYR"
     gene            3304..4311
                     /gene="pfkA"
                     /locus_tag="VW854_001673"
     CDS             3304..4311
                     /gene="pfkA"
                     /locus_tag="VW854_001673"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEV0262882.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGSPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            4370..5875
                     /gene="pyk"
                     /locus_tag="VW854_001674"
     CDS             4370..5875
                     /gene="pyk"
                     /locus_tag="VW854_001674"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEV0262883.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(6139..6387)
                     /locus_tag="VW854_001675"
     CDS             complement(6139..6387)
                     /locus_tag="VW854_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV0262884.1"
                     /translation="MQKANTKLEKPINQPLSSHIFRHTLLSTLAEKNAIMVRVEHKNA
                     KTVNNIYTHVSKRMEQATLEVLNTISLNRKYIRSNLDK"
     gene            complement(6554..6779)
                     /locus_tag="VW854_001676"
                     /pseudo
     CDS             complement(6554..6779)
                     /locus_tag="VW854_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7627..8499
                     /locus_tag="VW854_001677"
     CDS             7627..8499
                     /locus_tag="VW854_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEV0262885.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(8645..8989)
                     /locus_tag="VW854_001678"
                     /pseudo
     CDS             complement(8645..8989)
                     /locus_tag="VW854_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            9132..9443
                     /locus_tag="VW854_001679"
     CDS             9132..9443
                     /locus_tag="VW854_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262886.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEYPELYAVAQTSGEIPEKKREELRQIITTIFEQKMNKHSI
                     K"
     gene            9447..10163
                     /locus_tag="VW854_001680"
     CDS             9447..10163
                     /locus_tag="VW854_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV0262887.1"
                     /translation="MKKYFIGGLGSNAYHSKDFLQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSIGGDLARYFASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIISDLDVLTSKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNEATWREEALKELPDYFDTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(10293..11108)
                     /locus_tag="VW854_001681"
     CDS             complement(10293..11108)
                     /locus_tag="VW854_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEV0262888.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWISAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(11105..12010)
                     /locus_tag="VW854_001682"
     CDS             complement(11105..12010)
                     /locus_tag="VW854_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEV0262889.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGMFFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMILAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLVYSLLIGVIL"
     gene            complement(12256..>12433)
                     /locus_tag="VW854_001683"
     CDS             complement(12256..>12433)
                     /locus_tag="VW854_001683"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262890.1"
                     /translation="TDEVSSKHCFEVVDKTDEVSSKHCFEVVDKTDEVSSKHCFEVVD
                     RTDEVSNRTTVRRS"
BASE COUNT         3879 a         2177 c         2640 g         3737 t
ORIGIN      
        1 tgacaaaaat acaaatgaat agaaaataaa ttagtaagct gatgaaattt ttctcaagag
       61 aagctattta taggtgaaaa tggtataata tagtgagaag gatagaggag aagtgtaaat
      121 tgatcgcaca actagataca aaaacagtct atagttttat ggaaagcgtc atttcgatcg
      181 aaaagtatgt gagagcagct aaagaatacg gctacactca tttggctatg atggatattg
      241 acaatcttta tggcgctttc gactttctag agattacaaa aaaatacggc attcatcctt
      301 tgctagggct tgaaatgaca gtgtttgtag atgatcagga agtgaatttg cgctttttag
      361 ctctatctag tgtgggctat cagcagttga tgaagctttc gacagccaag atgcaggggg
      421 tgaaaacttg gtcagtcctg tcccagtacc tggaggatat cgcggtcatt gtgccttatt
      481 ttgatagagt tgagtcgtta gaactaggct gtgattacta tataggggtt tatccagaaa
      541 cactagcaag cgaatttcat catcctatct tacctcttta tcgggtcaac gcttttgaaa
      601 gcagggatag agaagttctt caagttttaa cagcgattaa agaaaatcta ccgctcagag
      661 aagttccctt gcgttcgaga caagatgtct ttatatcagc aagttcttta gagaaactat
      721 tccaagagtg ttttccgcaa gctttggaca atttagaaaa gcttatttca ggcatttctt
      781 acgacttgga tactagtctg aaactgcctc gttttaatcc agctagacca gcagtagagg
      841 agttgagaga gcgtgctgaa ctggggcttg ttcagaaggg gttgactagt aaagaatatc
      901 aagatagact agaccaagaa ttgtctgtta ttcatgatat gggctttgat gattatttct
      961 tggttgtttg ggatttgttg cgttttggac gatcgaatgg ctattatatg ggaatgggaa
     1021 ggggttctgc agtaggcagt ttggtttctt atgccttaga catcacgggg attgacccag
     1081 tagagaaaaa tctgattttt gaacgctttc ttaatcgtga acgctatacc atgcctgata
     1141 ttgatattga tatcccagat atttatcgtc cagattttat cagatatgtt ggtaataaat
     1201 atggtagtaa acatgcggca caaatcgtta ctttttcaac ctttggagcc aagcaagctc
     1261 ttcgagatgt tttgaaacgc tttggtgtgc cagagtatga attatctgca attactaaga
     1321 aaatcagttt tcgtgacaat cttaagtcgg cctatgaggg caatctccag tttcgtcagc
     1381 aaatcaatag taagttagaa taccaaaaag cttttgagat tgcttgcaag atagagggct
     1441 atccaaggca aacctctgtc catgcggctg gtgttgtaat tagtgaccaa gatttaacca
     1501 actacattcc tctaaagtat ggtgatgaaa ttccactgac tcagtatgat gctcatggag
     1561 ttgaggctag cggacttttg aagatggact ttctgggact acgaaatttg acctttgtcc
     1621 agaagatgca agagttgctt gctgaaacag aaggtattca tctgaaaatt gaagaaatcg
     1681 atttagaaga caaagaaacg ttagctttat ttgcctctgg taatacaaaa ggtatctttc
     1741 aatttgagca accaggtgcc attcgtctgc ttaagcgtgt gcaaccagtc tgttttgaag
     1801 atgtcgtcgc gactacttct ctaaatcgac cgggtgctag tgactatatc aataattttg
     1861 tggcaagaaa gcatgggcag gaagaagtga ctgttctgga tccagtactg gaggatattt
     1921 tggctccaac ctacggcata atgctctatc aggagcaggt tatgcaggtt gcccagcgat
     1981 ttgccggatt tagtcttggg aaagccgata ttttgcgtcg ggctatgggg aaaaaggatg
     2041 cctctgccat gcatgagatg agggcttcct ttattcaagg ttcattagaa gctggtcata
     2101 ctgtggaaaa agcagagcag gtctttgatg ttatggagaa gtttgcaggc tatggtttta
     2161 acaggtcaca cgcctatgcc tactcagcat tggccttcca gttggcttat tttaaaacac
     2221 attatccagc catattttat cagattatgt tgaattctgc caacagtgat tacttaatag
     2281 atgcacttga agcaggtttt gaagtggcgc ctctgtccat caacacgatt ccctatcacg
     2341 ataaaattgc caacaaggcc atctatctag gtttgaaatc cattaaagga gtcagtaatg
     2401 atttagctct ctggattatt gaaaatagac cttattctaa cattgaagat tttatagcta
     2461 aattacctga gaattatctg aaacttcctc tgctagaacc tttggtaaaa gttggtcttt
     2521 tcgattcatt tgaaaaaaat cgtcaaaaag tatttaataa cttagctaat ctatttgaat
     2581 ttgtgaaaga gttgggaagt ttgtttggag atgctattta tagttggcag gaatcggaag
     2641 attggacgga acaagaaaaa ttttatatgg aacaagagct tttagggata ggtgtcagca
     2701 aacatccact acaagctatt gcaagtaagg ctatttaccc gattacccca atcggaaatt
     2761 tgtcagaaaa tagctatgct attattttgg ttgaagttca gaaaataaaa gtgattcgta
     2821 ccaaaaaggg tgaaaatatg gccttcttac aggcagatga tagtaagaaa aaattggatg
     2881 tcactctctt ttcagactta tatcgtcagg ttggacagga aataaaagag ggagccttct
     2941 actatgtaaa aggaaaaata caatcacgtg atggccgtct gcaaatgatt gcacaagaaa
     3001 taagagaagc agttgctgaa cgcttttgga tacaggtgaa aaatcatgaa tcggatcaag
     3061 aaatttcacg tattttagaa caatttaaag gcccaatccc agtcatcatc cggtatgaag
     3121 aggaacagaa aaccatcgtt tctctccatc attttgtagc taaatccaat gaattagagg
     3181 agaaattgaa tgaaatcgtt atgaaaacga tttatcgcta aaaatacgga aaatagaaga
     3241 attttaaaat caaatgtgat ataatcaaga agaatgttaa aagaaaaagg agcataaacc
     3301 aatatgaaac gtattgctgt tttgactagt ggcggagacg cacctggtat gaacgctgct
     3361 atccgtgcag ttgttcgtca agcaatttca gaaggaatgg aagtgtttgg tatctatgac
     3421 ggatatgctg gtatggttgc cggtgaaatt catcccctag atgcagcttc agtaggagac
     3481 atcatttctc gtggtggtac ttttcttcac tcagctcgtt atccagagtt cgctcaactt
     3541 gaagggcaac ttaaagggat tgagcaattg aaaaaacacg gaattgaagg tgtagttgtt
     3601 atcggtggtg acggatctta ccacggcgct atgcgtttga ctgaacatgg ctccccagct
     3661 attggtcttc caggtacaat cgataacgat atcgttggta ctgactttac aatcggtttt
     3721 gacacagcgg ttactactgc catggacgct attgataaga ttcgtgatac atcatcaagt
     3781 caccgtcgta cttttgtaat cgaagttatg ggacgtaacg ctggtgatat cgctctttgg
     3841 gctggtattg caactggtgc tgatgaaatc atcatccctg aagcaggctt caagatggaa
     3901 gatattgtag caagcatcaa agctggttat gaatgtggta aaaaacacaa tatcatcgtc
     3961 ttagctgaag gtgtgatgtc agcggctgaa tttggtcaaa aacttaaaga agctggagat
     4021 acaagcgacc ttcgtgtaac agaacttgga catattcaac gtggtggttc tccaactgcg
     4081 cgtgaccgtg ttttggcgtc acgtatgggt gcacatgctg ttaaacttct taaagaaggt
     4141 atcggtggtg ttgcggttgg tattcgtaac gaaaaaatgg ttgaaaatcc aattcttggt
     4201 actgcagaag aaggggcatt gtttagcctt actgcagaag gtaagattgt ggttaacaac
     4261 ccacacaaag ccgatattga gctatctagc ttgaataaga gcttgtcata atttacaatt
     4321 tagttaataa gaccaaaaag gtcatatata taaaggagtc acaaaaatca tgaacaaacg
     4381 tgtaaaaatc gttgcaactt tgggtcctgc ggtagaaatc cgtggtggta aaaaattcgg
     4441 tgaggacgga tactggggtg aaaaacttga tgttgaagct tcagctaaaa acattgctaa
     4501 attgattgaa gctggtgcta acacattccg attcaacttc tcacacggcg accaccaaga
     4561 acaaggtgag cgtatggcaa ctgttaaact tgcggaaaaa attgcaggta aaaaagttgg
     4621 tttccttctt gatacaaaag gacctgaaat ccgtacagaa ttgttcgaag gtgaagctaa
     4681 agaatattca tacaaaactg gtgaaaaaat tcgtgttgca actaaacaag gaatcaaatc
     4741 aactcgtgaa gtgattgcgt tgaacgttgc tggtgctctt gatatctatg atgatgttga
     4801 agttggtcgt caagttttgg ttgacgatgg taaacttggt cttcgtgtgg ttgctaaaga
     4861 tgatgcaact cgtgaatttg aagttgaagt tgaaaacgat ggtatcatcg ctaaacaaaa
     4921 aggtgtgaac atccctaaca ctaaaattcc tttcccagct cttgctgaac gcgataacga
     4981 cgatatccgt ttcggtcttg aacaaggtat caacttcatc gcaatttcat tcgtacgtac
     5041 tgcaaaagat gtgaacgaag ttcgtgcaat ctgtgaagaa actggaaacg gacatgttca
     5101 attgttcgct aaaatcgaaa accaacaagg tatcgataac ttagacgaaa tcatcgaagc
     5161 agctgatggt attatgattg ctcgtggtga tatgggtatc gaagtaccgt tcgaaatggt
     5221 tccagtttat caaaaaatga ttatcaagaa agtcaatgct gcaggtaaag ttgttatcac
     5281 tgcaacaaac atgcttgaaa caatgactga aaaaccacgt gcaactcgtt cagaagtatc
     5341 agacgtattc aacgctgtta tcgacggaac tgacgctaca atgctgtcag gcgagtctgc
     5401 aaacggtaaa tacccactcg agtcagtaac tacaatggct acaatcgaca agaacgctca
     5461 agctcttctt aatgaatacg gacgtcttga ttcagattca tttgagcgta actctaagac
     5521 agaagtaatg gcttctgctg ttaaagatgc tactagctca atggatatca aattggttgt
     5581 aactcttact aagacaggtc atactgcacg tttgatttct aaataccgtc caaatgctga
     5641 catcttagca ttgacatttg acgaattgac agaacgtggc ttgatgttga actggggtgt
     5701 tatcccaatg ttgacagatg ctccatcttc aactgacgat atgttcgaaa tcgctgaacg
     5761 taaagcggta gaagcaggtc tcgttgagtc aggcgatgat atcgttatcg ttgctggtgt
     5821 gccagtagga gaagctgttc gcacaaacac aatgcgtatc cgcacagtac gttaagaaaa
     5881 atataaaaac ctatcatatc cagctttcta gcctgtatga tgggctcttt ttttgtccag
     5941 ggggcaaaac tttcataatg gattgatact cttcgaaaat caaattcaaa ccacgtcagc
     6001 gtcgccttac cgtactcaag tacagcttgc ggctagcttc ctagtttgct ctttgatttt
     6061 cattgagtat gaaataagat atgtacaaat tgattagaaa gtcaaatgaa tttctacaaa
     6121 tgttttagca atcgcaatct acttgtctag attcgatctg atatattttc gatttaatga
     6181 tatggtattt aaaacctcca aagtagcttg ctccattctt ttacttacgt gagtgtagat
     6241 gttatttact gttttagcgt ttttgtgttc cactctaacc attatagcat tcttctcagc
     6301 tagtgtacta aggagtgtgt gcctgaaaat atgggaacta aggggctggt ttatcggttt
     6361 ctctagttta gtatttgcct tttgcaaagt gatcttaaaa gcctttctct aaatttacat
     6421 atcactattg tttaacaaaa tctaatctat tttaggtcac ttattctttt tttgaaatgt
     6481 agaatgaact ttttcaaagt ttttcgaatc ttttaaaatc tgtttgtttt atatcgccat
     6541 tctcccccct tttttaattc tccctatata gcctgacaac tttcccgatg gtacgaatat
     6601 ggttgctttc gtctaggtgg atgtcggggt attcgggatt gagttttttt gaggtagcct
     6661 tggcggagtt tcttgacata gttagcgccg tctacttgga agatgccgat ggtattatag
     6721 tcaatctgtg gggtattctt gataaatagg tagtcgctgt ttcttatctt tgcctccatg
     6781 gacttgctga cgacataagc gattgggtcg tagtcgtctg ggataatgga aactccataa
     6841 ctaaatcgtc gtcctgcatc gagcggctac ctgcagagat aaactaccta acacgagagt
     6901 aagtagtctg tctgtagtcg tccagtctga tgatttttac gatacttcgt ttttctgctt
     6961 atacagttgc ctctaggcat aggttaggac tttaccttgt ctgggtggtg cccgttggtc
     7021 gtagatagat tggatatcgc taggagaatc cttttgaact ggaggaaaga gggcatcaat
     7081 caagctactg aatactttaa ctaagtcaaa tatagtattt ttcttagtag acctaaccct
     7141 tttttcataa tttctaatgg tgtttttact tatacctatc ttagtaccca attcttattg
     7201 agtccaacca ttactagtct atattgtttt atagttgatt gagtttggaa tagtacgctg
     7261 tagctgctaa aacatttcta ggaattaatt tgcctttcct aatagagttg ttcatatctt
     7321 atttcaatct attatatttt tcgcctctaa caatcgcaat ctcttcttta tctacgaacg
     7381 aaatcgcttt ctatttttgt aagtaaagca tcacacgaaa tccacgaaaa tgaaaacctt
     7441 tgttgtgttt tcgtaaaaaa tttgttgaca gagcacgaaa cgcctactat aattaaatca
     7501 agtttaagga aataacaaaa aaacaaatcg gagggaaaga cgatgaacac attaaatgag
     7561 aaagtaatca atatctgtaa agcagtagtt aaagaaactt taatccaaga catttaggag
     7621 aaagaaatga agaaatcaag aaaattagcc acattaggaa tctgttcagc cttgttttta
     7681 ggcttggctg cttgtcaaca acaacatgct acttctgagg gtacgaatca aaggcaaagc
     7741 agttcagcga aagttccatg gaaagcttca tacaccaacc taaacaacca ggtaagtaca
     7801 gaagaggtca aatctctctt atcagctcac ttggatccaa atagtgttga tgcatttttt
     7861 aatctcgtta atgactataa taccattgtc ggctcaactg gcttatcagg agatttcact
     7921 tcctttactc acaccgaata cgatgttgag aaaatcagtc atctctggaa tcaaaagaag
     7981 ggcgatttcg ttgggaccaa ctgccgtatc aatagttatt gtcttttgaa aaattcagtc
     8041 accattccaa agcttgaaaa gaatgaccag ttgcttttcc tagataatga tgcgattgat
     8101 aaaggaaagg tctttgattc acaagataag gaagagtttg atattctatt ttcgagagtt
     8161 ccaactgagg caactacaga tgtcaaggtt cacgctgaaa agatggaaac attcttctca
     8221 caatttcaat tcaatgaaaa agctcgaatg ctgtctgtag tcttgcacga caatctggat
     8281 ggcgagtatc tgtttgtagg ccacgttggg gtcttagtac ctgctgatga cggtttctta
     8341 tttgtagaga aattgacttt cgaagagccc taccaagcga ttaaatttgc tagtaaggaa
     8401 gattgctaca agtatttggg caccaagtat gcggattata caggcgaggg actggctaag
     8461 ccttttatca tggataatga taagtgggtt aaactttaat agtgttatga tatgacagta
     8521 atcttaggat ttttatttct atttttgata agttggtggg ttatttcaat cgttaatagc
     8581 cagaaaccac gcaaaaatga aacatttatt ggctatgtcc aaagatacga tattgatggg
     8641 aatggctatc aatcattacc gtgtcctcag aactgagagg agttcttgaa atcgtaaaac
     8701 cactttgaac aagagttact tcaacccatt ggctccgacg gattaagttg ctttcgtgaa
     8761 taccaaaatc agccgcaatt tgttcataag tgcgctattc tcgcacatat tgaagagtgg
     8821 ccataagaag gtcttctagg cttaatttag gttttcgtcc accttttgcg tgtttaagtt
     8881 gataagctgt ttttaataca gctaatatct cttcaaaagt cgtgcgctga acaccaacaa
     8941 gacgtttaaa tcgtgcatca gttagttgtt tacttgcttc ataattcata gaactactat
     9001 accatatttt gtttcgcagg aagtctaatg ctaaaatctt atcagtgaga agatttctta
     9061 aagtttattc tttgtagttt catctgtatt agtacataat ttaacttaga gaaaatagga
     9121 ggactaatcg aatggaatta aaagatttta cagaaaaaga acaggaaatg attaagaaaa
     9181 ggcttacaat gtctaatatt agtgacaaag aaactactga gaagattctg gcgctagtcc
     9241 cacaagactt gattaagcga atcccgtttt ttgtcagaaa acatgctaca acacgtacga
     9301 ttaaacgcat ttcaattgaa taccctgaac tctacgctgt agctcaaaca agtggtgaga
     9361 ttccagaaaa aaaacgcgaa gaattgcgtc agattatcac aactatcttt gaacaaaaga
     9421 tgaataagca tagtattaag tagagaatga aaaaatattt tattggcggt ttgggaagca
     9481 atgcctatca tagcaaggat tttcttcaag aactagattc gcaggtctat tttctaaatc
     9541 catatgaaaa gcatcttcga gatgaaacag aattgaaatc atggtttaaa aatgagattg
     9601 tagaggaaga atctatctgt ctgataggtc attctattgg aggcgattta gctcgttatt
     9661 tcgcatcgga atttgaagaa gtaaagaaac tgattctttt ggatggtggc tatctagatt
     9721 tagataagat tttacctttg gatacagagt tagaggaaac taaaaattat atcaaatctc
     9781 aaattatttc ggacttagat gttcttactt ctaaagaaaa atctgaagca aagcattggt
     9841 cagaaaatat ggagaaagct gtaagacagt cctatcactg gaatgttgag tataatagat
     9901 atgagttggc tataaattat gaaaatatag aagcgatact ccgcctacgg agaaaaatac
     9961 aagcttttaa gagagaagtg ggagatacct tgtttattag tcctcgctat cctaatgaag
    10021 ctacatggag agaggaagcc ctaaaagaat tgccagacta ttttgatact atttttttag
    10081 aaaactttgg ccatgagctt tatactcaag cacctaaaga aatcgctagt ctaatgaatg
    10141 agtggctcgc ttattttcta tgagctactt gtattgctgg aaatggtata agatgaaaat
    10201 ctacaagtca ctaattttca tataaccaaa aaacgaacag gctctttttc tgttattcag
    10261 aagagaactt gttcgttttt atcttgattt tcttaaaaag ctctatatac atagggattt
    10321 tctggtttat cgactttggt aaagctgtcg atttccaagg ttggaaggtt ttgtttgagt
    10381 tctttatggt agtgattgac cagttttcct ttggctgaaa tccaagtgtt gttttcatac
    10441 tgccgggtat tgcccttggt cagcaatcca tagacaccag aatctgcgat gcagtgaata
    10501 atgccgaagc ggaacagaaa ttggctattg gcatgactgg ggtcattata gacaaagcct
    10561 gtgaactgga ttgtcttacc ctcaaattca tctggatagt cgtagatagc ctccatgacc
    10621 tccatatagt tttcattagt gatctgaata ctatcttggg ataagtattt atccgccgcc
    10681 gttcgcattt ccttttcata ggctgatttt gaaaaataag aactggtatc tggtttcaaa
    10741 tattggcttg tcgtcccttc gcttgtctga atggctagat ccgttccttc cgataggggg
    10801 aaatgataac ctttagcaga aacggtctga gaatccaagc taacagttgg gaaagttaag
    10861 ccgatgacaa gtggaatagc cagaagagaa atactcgtca tcttggctaa tcggctgttc
    10921 agatgactgt gggttttgac ttgcttcatc cagatataca attgaacgat agccaagata
    10981 aaagaaagca ccatggagat ataggccaga taggaatagt gcatgttgat gtactggttt
    11041 agtttgcccg acagatggag gtaaatagtc agttcaaaat agccagctaa aactaaaaat
    11101 cgaatcatag gatgactcca atcaagagag aatagactaa gacgacaaga gttacaattg
    11161 ttatgaagtg actgataaat cgtgctttca agtaattttt catcatgaga atatttttga
    11221 tatccagcat tggaccaatg acgagaaagg ccagaactgg tgccaaaccg aaactcgaga
    11281 gaagagaagc acctataaag gcatccgcct cactacagag cgaaagaaga aaggctaaaa
    11341 tcatcaagag caggatggca aaaagagggg tcgcactgat agaggtcaga atccgagtcg
    11401 gaacgtagac ctgtattata gaagcaaaga ggcagccaaa taccaaataa cgccccgtat
    11461 caaaaaattc atcaatggcc tgcacaaaga cttgaaaaac tttttttgca gaactcaagt
    11521 aagaaaaatc atgctcatga caagccagac gattttcttt ctgaatcggt tcttgccaga
    11581 aaaatcctag aaaaattcct agtattacag ccacaagaat ggaacccaga gctcgtaata
    11641 gggcgacatg gaaggagttg ccaaaggcag aataggtcgc aaaaagaaca atgggattga
    11701 taacaggtgc tgtcacaaga aaaggaacgg ccgtgtaact tggaaccttt ttttccagaa
    11761 aacgattgat gatggggacg attccacatt cacaagaagg gaaaaacata ccgacgaagg
    11821 tcccaaaaaa gattctcccc caacgatttc gagggagaaa atgataaacc ttgtcaggtg
    11881 tgatataaac ttcaatcagc cctgagacaa tgcttcctat cagaacgaag ggaagggctt
    11941 caatgataat ggagagaaaa atggctccag tttgaagtac actagatggg agttgttgaa
    12001 aagcagtcat ctagtttttc ttttctgctt tggctttttt ctctttgtca tcagggaaag
    12061 taacttgttt caaatcaggg agtttagcaa actcttcaaa catcttgtcc aagtcgtctc
    12121 gttttgtaaa tttgacagcc ataggtgtac ctcgattcta aattctacct ctattatact
    12181 attattttgc ggaaaaggga gttttttaat ttgttttcga tagttgatac acttcgaaaa
    12241 tctcttcaaa ccacgtcagc ttcgccttac cgtagtacgg ttactgactt cgtcagttct
    12301 atccacaacc tcaaaacagt gttttgagct gacttcgtca gtcttatcca caacctcaaa
    12361 acagtgtttt gagctgactt cgtcagtctt atccacaacc tcaaaacagt gttttgagct
    12421 gacttcgtca gtc
//
LOCUS       DAVKWZ010000055        12121 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000055 DAVKWZ010000000
VERSION     DAVKWZ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12121
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.055"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(49..762)
                     /gene="rsmG"
                     /locus_tag="VW854_001684"
     CDS             complement(49..762)
                     /gene="rsmG"
                     /locus_tag="VW854_001684"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEV0262891.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            856..1416
                     /locus_tag="VW854_001685"
     CDS             856..1416
                     /locus_tag="VW854_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEV0262892.1"
                     /translation="MTWIILGVIALVVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLKKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            1418..2317
                     /gene="htpX"
                     /locus_tag="VW854_001686"
     CDS             1418..2317
                     /gene="htpX"
                     /locus_tag="VW854_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEV0262893.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSGMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(2361..3920)
                     /locus_tag="VW854_001687"
     CDS             complement(2361..3920)
                     /locus_tag="VW854_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV0262894.1"
                     /translation="MHYEHSRKGNHMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKTLMGEDLSDFTIKGDIQSDYQSLAYIPQKVPEDLKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKTAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLAQELQLTVRGQEKIGIIGPNGIGKSTLLAKLQQLLNDKREISLGFMPQDYHK
                     KLQLDLSSIAYISKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSSIYRLTE
                     HGLELVNLEDV"
     gene            complement(4335..4475)
                     /locus_tag="VW854_001688"
     CDS             complement(4335..4475)
                     /locus_tag="VW854_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blpT protein"
                     /protein_id="HEV0262895.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQVTADDIRLQELLNTTMQELKKQ
                     KS"
     gene            4645..5187
                     /locus_tag="VW854_001689"
     CDS             4645..5187
                     /locus_tag="VW854_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEV0262896.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGALDLAESVSDNILLNIPIKVLTAEEEAGQGFVSGNDWQIMTEEEYQAQQA
                     VKKEENSPFAGLQGLFDGDE"
     gene            5187..5816
                     /gene="nth"
                     /locus_tag="VW854_001690"
     CDS             5187..5816
                     /gene="nth"
                     /locus_tag="VW854_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEV0262897.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            6027..6548
                     /gene="pyrR"
                     /locus_tag="VW854_001691"
     CDS             6027..6548
                     /gene="pyrR"
                     /locus_tag="VW854_001691"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEV0262898.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            6567..7490
                     /locus_tag="VW854_001692"
     CDS             6567..7490
                     /locus_tag="VW854_001692"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEV0262899.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGKFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            7540..8619
                     /gene="carA"
                     /locus_tag="VW854_001693"
     CDS             7540..8619
                     /gene="carA"
                     /locus_tag="VW854_001693"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEV0262900.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            8932..12108
                     /gene="carB"
                     /locus_tag="VW854_001694"
     CDS             8932..12108
                     /gene="carB"
                     /locus_tag="VW854_001694"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEV0262901.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPVQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLETGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDQVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKDVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
BASE COUNT         3455 a         2391 c         2685 g         3590 t
ORIGIN      
        1 aaataagaga gcgaggttga ttttgtaaat atttttttac ttaaaaattt aaagcgggcg
       61 tttatttggc ataccagcct tacgtggata tttatttggt gtttcttttt tcttttctac
      121 cactgtgatg taacgcggat ctctatttgg tagggcgtag ctgagattgt cttcgacttt
      181 actaaaaagg agattgaggg cattcttagc ttctaacaac tcctcaggcg cattgctggc
      241 cttgagtgcc aatagttttc caccaacctt aaggtaggga atagtcaatt cagataggac
      301 ctgcatacgg gcaaccgcac gagctgttac aaaatcatat tgagcacgga agttcttgtc
      361 ttgggcaaaa tcttcggcac gtccgtggta gaaatgaact ccgttcaaat ccagttcttg
      421 agccaagagt tgtaggaagt tgatgcgctt attgagtgaa tcaataatgg tcacatctaa
      481 ctccggatag agaattttca ttggtagact aggaaatcct gccccagccc cgatatcaag
      541 aagtttgata gtttcattgg gaatcaaacc ttgaagaatg ggtgcaatcg aatcgtaaaa
      601 atgtttgaga taaacttctt ccttgtccgt aatcgccgtc aaattaatct tctcattcca
      661 ctcgaccaag agctcaaaat aacgttcaaa ttgttctttt tgctggtccg aaagtggaag
      721 attctgctcg gcaagcaagt tgtaaaatgt ttttggtttc atagttattt ccttctttag
      781 tatcatctta ttttaccaca atcatagaaa atttgatata ctggtactaa tctaaaagca
      841 gaaaggactt atattatgac ttggattatt cttggagtta tcgctcttgt tgttattttt
      901 gtgattgtta gctataacgg tttggttaaa aatcgtatgc aaaccaagga ggcttggagt
      961 cagattgatg ttcagttgaa acgtcgcaat gatctcttgc caaacttgat tgagactgta
     1021 aaaggttatg ccaaatatga aggttctacc cttaaaaagg tggcagaact acgtaaccaa
     1081 gtggcggcag cgacttcacc agcagaagct atgaaagcca gtgatgccct cactcgtcag
     1141 gtttcaggta tttttgcagt tgcagaaagc tatccagatt tgaaagctag tgctaacttt
     1201 gttaaattgc aagaggagtt gacaaacaca gaaaataaaa tttcttactc tcgtcaactc
     1261 tataacagtg ttgtcagcaa ctacaatgta aaattagaaa ctttcccgag caatattatc
     1321 gctggaatgt ttggatttaa agcggcagat ttccttcaaa cacctgaaga ggaaaagtcg
     1381 gttcctaaag ttgattttag cggtttaggt gactaagatg ttgtttgatc aaattgcaag
     1441 caataaacga aaaacctgga ttttgttgct ggtatttttc ctactcttag ctcttgttgg
     1501 ttatgcggtt ggttatctct ttataagatc tggacttggt ggtttggtta tcgcactgat
     1561 tatcggcttt atctacgctt tgtctatgat ttttcaatcg acagagattg tcatgtccat
     1621 gaatggagcg cgtgaggtgg atgagcaaac ggcaccagac ctctaccatg tagtggaaga
     1681 tatggctctg gtcgcccaga ttcctatgcc ccgtgttttc atcattgatg atccagcctt
     1741 aaatgccttt gcgacaggtt ctaatcctca aaatgcggct gttgctgcga cttcaggtct
     1801 actagctatc atgaatcgtg aagaactaga agctgttatg ggacatgaag tcagtcatat
     1861 tcgtaattat gatatccgta tttcgactat tgcagttgcc cttgctagtg ctatcaccat
     1921 gctttctggt atggcaggtc gtatgatgtg gtggggtgga gcaggtcgca gacgaagtga
     1981 tgatgaccga gatggaaatg gtcttgaaat cattatgcta gtggtttccc tactagctat
     2041 tgtactggca cctctcgctg caaccttggt tcagctcgct atttctcgtc agagggaatt
     2101 tctggcagat gcatctagtg tcgagctgac tcgcaatccc cagggaatga ttaatgccct
     2161 agataagttg gacaatagta aacctatgag tcgccacgtc gatgatgcta gcagtgctct
     2221 ttatatcaat gatcccaaga aaggtggggg gttccaaaaa ctcttttata cccacccacc
     2281 tatctcagaa cggattgaac gtttaaaaca gatgtaaaag aatccagagg tcgtctctgg
     2341 atttttgcta tgttgcaaat ttatacatct tctaaattaa ctaactccaa accgtgttct
     2401 gtcaagcggt agatgcttga acagacctct tttaagaaac gacggtcatg cgaaacagtg
     2461 atgagaccgc ctggataggt tgcaaagagt tttctgattt ggggttgaga agtgggagaa
     2521 aagtttcgtg tgggttcatc cagcaggaga aagtttggtt tgcgcaggac taaatccaaa
     2581 agcaggagtt ttccctgttg tccgccagat aaggagcgaa tttgatgctg catttctgga
     2641 taactgaaat tgagactggc taggtgagat tggattttct gaagttcctc tttttcccca
     2701 gttttgctga tataagctat tgaggataaa tccaattgca gttttttgtg gtaatcttgt
     2761 ggcataaaac caagtgaaat ctctctttta tcattaagaa gttgctgtaa tttggctaac
     2821 agagttgatt tcccaatacc atttgggccg atgataccga ttttttcttg gccacggaca
     2881 gttagttgca gttcctgagc caaaacacgg tcgtcaatgg acaaattttc tttttccagt
     2941 tggactaaga ctttagaagc tggtaatggt tggatgtctg aaaagaaaag ttggatttgt
     3001 tcctcttcaa gtggcttttg agtcatggac tgagctgtct tttcgtagcg tttttcttgt
     3061 gagaggacag ttttcatctt tttagccaat aggcgaccgg cagtactatc tttggtagct
     3121 cgaagcgcag tttctacatt ttgcttaact ctccgatgtt tttccatggt tttatcgtag
     3181 gctctttggt tgttagcagc ttgctgactt tgtttggcaa aattagcctt tctctgctca
     3241 ctatagctat catagtctaa atgctctact agcgtttccg cttctttacg gtgtttgacc
     3301 agtcgcaagt ggacaatagt gtctgccgtt tcagaaagaa agtcttcatc atgggaaatg
     3361 aaaataacgg tttgcctggt cttttgaatc tggcctttta gccaatcaac tgtctcaagg
     3421 tctaggtcat ttgaaggttc atctaaaaat agaatctcaa agggtttggc taactcatgg
     3481 ataagctgaa ttttcaaagc ttcgccccct gatagattgc caatctcttg gtcacttgcg
     3541 aaacgattgc tatcaaaatg caattcctcc gccaaacgat agaggatact gtagtctaaa
     3601 tcaatagaat ctaaaaagaa gtagtcgtgt aaagttttct tttttaggtc ctcagggact
     3661 ttttgaggaa tgtaggccag tgactgataa tcagactgga tgtctccctt gatagtgaaa
     3721 tcagacaaat cttcccccat taaagtttta agtaaggttg atttaccatt tccttcttcg
     3781 ccaataatag ctaccttttc cccgtcttgt atggtcatgc ttaggtcaga gacaaggtct
     3841 cgtaaatctt tgttttgtgt gatggtcagg tgattgattt ttatcatatg atttcccttt
     3901 ctagaatgtt catagtgcat aacaaaaagc tctactttac ctagtagagc ccaaagtcta
     3961 tgccaaaaaa actctattaa catgccagaa aaagagcata aaagagcaca caaaaagaga
     4021 caaaaacaag atctactaaa taaaggttct ttatttgata gacttagttg ttcatgtctc
     4081 ccttacctcc gagtattagt atctttatat tataccttta aggaaatttt gtcaacagaa
     4141 aactggaaat tctatgctct aaaatccaaa aacaggtcca tagagactta gtacgtgttt
     4201 gacatactcg taatggattt tatcatagtt tcgccaagca gtgcgtgctt gattgtttag
     4261 ttttaggcta cccagtccaa tcagaggact ggtgggttgg taaaatttct caagattaat
     4321 taagatactg ttgttcaact tttctgtttt ttgagctctt gcatagtagt gtttagcagc
     4381 tcttgtagtc gaatatcatc tgccgtaacc tgtttacagt tttggtagct tttatttaac
     4441 ttggatagga gttgaatgag tttcttgtct tccatgtgag catcctcctt gatctgatgt
     4501 actctttccc tatagtatag cactaaaaaa taaaactatc atagtaaata tgttaaaatt
     4561 ttgaatttta attacaaagt ttggtgtgct gatttgtagc cttttcatgg tataatcaag
     4621 agagtaaatc tttatggagg aagtatgaaa ttaaatattc aagaaattcg taagcagtct
     4681 gaaggtttga actttgaaca aacgttagat ttagttgatg acctgcgtgc acgtaatcaa
     4741 gaaattttag atgtaaaaga tatccttgca gttgggaaag tacaatatga agaccgtatg
     4801 tatttcttag attatcaact atcttatacc attgttcttg cttcgagtcg cagtatggag
     4861 ccagttgagt tagttgaatc ttatccagtc acggaagttt tcatggaagg cgcaactaac
     4921 cagctagatc aagaagtttt agatgatgac ttggtcttgc ccatcgaaaa tggggcgctt
     4981 gaccttgctg agagtgtatc agacaatatc ctgctaaaca ttcctatcaa ggtcttgacg
     5041 gctgaagaag aagctggtca aggatttgtc tcaggaaatg actggcaaat catgacagag
     5101 gaagaatacc aagctcaaca agcagtaaag aaagaagaaa acagtccttt tgctggctta
     5161 caaggactat ttgacggaga tgaataatgg tcttgtcaaa aaaacgagca cgaaaggtgc
     5221 tagaagaaat cattgccctc ttcccagatg ccaagcctag tcttgatttt accaatcatt
     5281 ttgaactcct ggttgcggtc atgttatcag cccagacgac agatgcagcg gtaaataagg
     5341 ccacaccagg tctctttgtt gcctttccaa caccccaagc tatgtctgta gcgacagaga
     5401 gtgagattgc ttcacatatt tctcgcttgg gattgtatcg gaataaagct aaattcctta
     5461 aaaaatgtgc ccaacagtta ctagacgatt ttgatggtca agtccctcag acacgtgaag
     5521 aattggagag tttggcaggt gttggtcgca agacagccaa tgttgtcatg agtgtaggat
     5581 ttgggattcc agcctttgca gtggatactc atgtggagcg tatttgcaaa caccacgata
     5641 ttgtcaaaaa atcagcgacg ccacttgagg tggaaaagcg ggtcatggat atcttgccgc
     5701 ctgagcagtg gttagctgcc catcaggcca tgatttattt tggaagagcc atttgccatc
     5761 caaaaaatcc agagtgtgac cagtacccac aattatatga ttttagcaat ttgtaagatg
     5821 ctacattgaa actttttgct tgattttaag catgctttgt gctatagtaa atgataacta
     5881 aatattcctt taaacctgtc ccgtgaggca ggcaaggagc gcgataaagt agaagggtga
     5941 agtctatact gtgttcagta gggctcgctt ggctataaat ttcaagtctc cttgtttaag
     6001 gagacttttc tttttggagg tttgtcatga aaacaaaaga agttgtagac gaattgaccg
     6061 tcaaacgagc gattacgcgt attacttatg agattatcga acgcaacaaa gatttgaata
     6121 agatcgtctt ggctggtatt aaaactcgtg gtgtctttat tgcccaccga atccaagaac
     6181 gtttgaagca gttagaaaat ctttcagttc ctgttgtgga attggatact aaacctttcc
     6241 gtgatgatgt taaaagtgga gaagatactt ctttggtttc tgtcgatgtg acagaccgcg
     6301 aagttatctt ggtggatgat gtgctctata caggtcgtac catccgtgct gctattgata
     6361 atattgtcgg tcatggtcgt cctgcgcgtg tgagtttagc agttctagtc gatcgtggac
     6421 atagagaatt gccaatccgt ccagattacg ttggaaaaaa tatcccaacc agtcgttctg
     6481 aagaaatcat cgtagagatg acagaacttg atgaccaaga cagagttctg attactgaag
     6541 aagcttagaa agttaaagga gtagccatgt cagaaaatca acaagcattg aaccatgtgg
     6601 tgtccatgga agacctcact gtcgatcaag taatgaaatt gatcaagcga ggaattgagt
     6661 ttaaaaatgg agcccagctt ccctatgaag accatccgat tgtttccaat ctcttctttg
     6721 aggattctac acggacacat aagtcctttg aagtcgcaga gattaaactt ggattggaac
     6781 gacttgactt tgatgtgaag actagctcgg ttaataaggg tgagacactt tatgacacca
     6841 ttttgactct gtctgcttta ggagtggatg tctgtgtgat tcgccatcca gaggtcgact
     6901 actatagaga attgattgct agtccaacga ttacgacttc catcatcaat ggtggagatg
     6961 gttcgggcca acaccctagc cagagcttgc ttgatttgat gaccatttat gaggaatttg
     7021 gccactttga gggtcttaaa gttgctattg caggtgactt ggaccactca cgcgttgcca
     7081 aatccaatat gcagattttg aaacgcttgg gagctgaact ctttttcgct ggacctgagg
     7141 aatggagaag tcaagagttt gcagactatg gaaagtttgt aaccattgat gaaatcattg
     7201 atcaggttga tgtcatgatg tttctccgtg tgcaacacga acgccatgat agtggagctg
     7261 tattttcaaa agaagactac catgcccaac atggcttgac tcaagaacgt tacgatcgtt
     7321 tgaaagaaac agcaatcctc atgcacccag ctccaatcaa tcgtgatgta gaaatagcag
     7381 accacttggt tgaagcacca aaatcacgga ttgtccaaca aatgaccaat ggtgtctttg
     7441 ttcgaatggc aatcttagaa tccgtactag cgagtagaaa cgccaactaa aacacaagac
     7501 gttaaaagac gccagtgagc gtccacgaga ggtgaaaata tgacaaaaag aattctagta
     7561 ttagaagatg gcacagtttt tgaaggcaag gccttcggag cagatattga tgtaacaggc
     7621 gaaatcgtct ttaatacagg gatgaccggc taccaagaat ccattacaga ccagtcttat
     7681 aatggacaaa tcttgacctt tacttatcct ttggtaggaa attatggtat taatcgtgat
     7741 gattatgaat ccattattcc aacttgtaag ggagtcgttg tttttgaaga agcgcgtaga
     7801 gctagcaact ggcgcaacca aatgaccttg gatgaatttt tgaaagccaa gaaaattcca
     7861 gggatttcag gaattgatac gcgtgctctt accaagatta tccgtaagca tggtactatg
     7921 cgtgcaacct tgacccatgt tggggacagt atggaccatg tgacggacca gctccaagca
     7981 acagttttac caacagacaa tatcaaacag gtttctacta aaacgtctta tccagctcca
     8041 ggagttggtt taagcgtggt gctagtggac tttggtctca agcactcaat cctacgtgaa
     8101 ctttctaaac gcaactgtaa cgtgacagtt gttccttatt cgacaacggc tgaagaaatt
     8161 ctccatctca atcctgacgg agttatgttg tcaaatggtc caggtaaccc agaagacgtt
     8221 ccacaagcac tcgacatgat tcgcggtgta caaggaaaaa ttccaatctt tggtatttgt
     8281 atgggacacc aactctttgc aatggcaaac ggggctaaga cctataagat gaagtttggc
     8341 caccgtggat tcaaccatgc ggtacgtgaa atcgcaacag gacgagtaga cttcaccagc
     8401 cagaaccatg gttatgcagt cagccgcgag gatttgccag agcacttgat tattacccac
     8461 gaagaaatca atgacaagtc agtggaaggt gtgcgccaca gataccaacc tgctttctct
     8521 gttcaatacc acccagatgc agctcctggg ccacacgacg ctagctacct atttgacgag
     8581 tttatcgaga tgatggaagt ttttaaacaa tcaaactaag aacgagtaaa agctcgtcgg
     8641 agaatttatg taactgaaca cggacggaaa gctcagaatg tagagaaacc aacttggatt
     8701 gtcaatcctt ccttgatttt ctaaaattca atcgctttct attcgtcctt tgtatcttat
     8761 ttaatgtcgc cctgagaggc gacgaataga aaggagaagg gtgctccgta tttgctgaac
     8821 tgaatacggg ctacggacgg tgctaaaaag atagttattc ctagaactga cagttcttcg
     8881 tcataactcc tattttagct ttgtccgctt gacgccctta atatcttata tatgcctaaa
     8941 cgtactgata ttcaaaaaat tatggtgatt ggttctggtc cgattattat tggtcaggct
     9001 gctgagtttg actacgctgg gactcaggct tgcttgtcgt tgaaagagga aggttatgag
     9061 gttgttttgg ttaactcaaa tcctgcaacc atcatgacgg acaaggaaat tgctgacaag
     9121 gtttacattg aaccgattac acttgagttt gtgacacgta ttcttcgtaa ggaacgtcca
     9181 gatgccttgc taccaacact cggtggtcag acaggtctca acatggccat ggaattgtct
     9241 aaaaatggta tcttagatga gcttggcgtt gaacttctgg gtaccaaatt atctgccatt
     9301 gaccaagcgg aggaccgtga cctctttaaa caattgatgg aagagcttga acaacccatt
     9361 ccagaatctg aaattgttaa cacagtagaa gaagcagttg cctttgcagc gacaattggc
     9421 tacccagtca tcgttcgtcc agcctttacc ctcggtggta ctggtggtgg tatgtgtgcc
     9481 aatgagaaag aattgcgtga aatcactgaa aatggtttga aattgtcacc tgttacccaa
     9541 tgtttgattg agcgttcgat tgccggtttc aaggaaattg aatacgaagt gatgcgtgac
     9601 tcagctgaca atgccttggt tgtttgtaac atggaaaact ttgacccagt tgggattcac
     9661 acaggggatt ccattgtatt tgcccctgtg caaaccatgt cagactatga aaaccaaatg
     9721 cttcgtgatg cgagcttgag cattattcgt gccctcaaga ttgaaggtgg atgtaatgtt
     9781 cagctagctc ttgatcctaa cagcttcaag tattatgtta tcgaagtaaa ccctcgtgta
     9841 tcacgttcgt cagcccttgc ttctaaagcg actggatatc cgattgctaa gttggctgcc
     9901 aagattgcag taggtttgac cttggatgag gttataaacc cagttacagg ttcaacctat
     9961 gccatgtttg aaccagccct tgactacgta gttgctaaga ttccacgttt cccatttgac
    10021 aagtttgaaa agggtgaacg ccgtctcggt acacaaatga aggcgactgg agaagtcatg
    10081 gcaatcggtc gtaatatcga ggaatctctt ctcaaggcct gccgctccct tgaaactggg
    10141 gtgcaccaca atgaaatacc tgaacttgca gcagtttctg atgatgcctt gattgaaaag
    10201 gttgtcaaag cccaagatga ccgtctcttc tacgtatcag aagcaattcg tcgtggctac
    10261 acacctgaag aaattgcaga gctaacaaaa attgatatct tctatcttga taaactcttg
    10321 catatctttg aaattgagca agaattgggt gctcatccac aagatctaga agttttgaaa
    10381 acagccaaac ttaatggttt ctcagaccgt aagattgctg aactctgggg aacgactgat
    10441 gatcaagttc gccaacttcg cttggaaaac aagattgttc cagtttataa gatggtagat
    10501 acttgtgcgg cagagttcga ctctgaaacg ccatatttct attcaaccta tggttgggaa
    10561 aatgaatcta tcagatctga taaagaatct gtacttgtcc taggttcagg tccaatccgt
    10621 atcggtcaag gggttgagtt tgactacgca accgttcact ctgttaaggc tatccaggca
    10681 gctggttatg aagctatcat catgaactca aacccagaga ccgtttcaac agacttctct
    10741 gtatcagata agctttactt tgagccattg acattcgaag atgttatgaa cgtcattgac
    10801 ttggaacaac caaagggagt tatcgttcag tttggtggtc aaacagccat caaccttgcg
    10861 gagccattgg caaaagcagg tgtgaccatc cttggtacac aggttgctga cctagaccgt
    10921 gccgaagacc gtgacctctt cgagcaagct ctcaaagaat tggatattcc acagccacca
    10981 ggacaaacgg ctaccaatga agaagaagca gctcttgcag ctcgtaagat tggcttccca
    11041 gttcttgtcc gcccatctta tgtacttggt ggacgtgcca tggaaatcgt tgaaaacgaa
    11101 gaagaccttc gttcttacat gcgaactgct gttaaggcta gtccagatca cccagttctt
    11161 gtcgactctt atatcgttgg gcaagagtgc gaagttgatg ccatttcaga tggtaaagat
    11221 gtactcattc ctggtatcat ggagcatatc gaacgtgctg gtgtccactc aggtgactca
    11281 atggctgttt acccaccaca aaccttgtcg caaaaagttc aggagaccat cgcagactac
    11341 accaaacgct tagcaatcgg tcttcactgc cttggaatga tgaacatcca gtttgttatc
    11401 aaggatgaaa aagtctacgt tattgaggtc aatccacgtg ccagccgtac cgttccattt
    11461 ctatctaagg taaccaatat tcctatggct caagtagcga ccaagctcat tcttggtcaa
    11521 agtttatcag aacttggcta ccaaaatgga ctttaccctg aaagcacccg tgttcatatc
    11581 aaggcacctg tcttctcctt tactaaacta gctaaggtag acagcttgct cggtcctgaa
    11641 atgaagtcaa caggtgaagt tatgggttct gatgctactt tggaaaaagc tctctataaa
    11701 gcctttgaag cttcttatct acacttgcca acttttggta atgtcgtctt taccatcgct
    11761 gatgatgcca aagaagaagc cttgaacttg gctcgtcgtt tccaaaacat tggttatgga
    11821 atccttgcga cagaagggac agcagccttc tttgctagtc atggcttgca agcccaacct
    11881 gttggtaaga ttggtgatga cgataaggat atcccaagtt ttgtacgcaa aggaagaatt
    11941 caagctatca ttaacacagt aggaacaaaa cgaactgctg acgaagatgg tgagcaaatt
    12001 cgtcgttcag ccattgaaca cggggtgccc ctcttcacag cgctagatac agctaatgcc
    12061 atgcttaagg tacttgaaag tcgtagcttt gttacagaag cgatttaaat agaaaaataa
    12121 g
//
LOCUS       DAVKWZ010000056        11991 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000056 DAVKWZ010000000
VERSION     DAVKWZ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11991
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.056"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(144..1775)
                     /locus_tag="VW854_001695"
     CDS             complement(144..1775)
                     /locus_tag="VW854_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEV0262902.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            2068..7008
                     /locus_tag="VW854_001696"
     CDS             2068..7008
                     /locus_tag="VW854_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEV0262903.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFVGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHTRENKLEKAEGVATASETAEAASAAKPEEK
                     ASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAAEANKTEKEVQPDVPKNTEKT
                     LKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNEKASKEAKVQALSN
                     TNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDAGHRNGVPVYGTLF
                     FNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFINQETTGDLVKPLG
                     EKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYNYQFMQPEGDKVPA
                     DNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKTKVKWNDILDENGK
                     LRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGDKDWYGIANLVADR
                     TPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWRWWQTSTGEKLRAE
                     YDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVAHKGGKGSKVYMAF
                     STTSDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFFEHEGAVKDYQFNL
                     GQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADFYEVYEKDGDSWKL
                     LTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTTFDWGMTVKDTSLP
                     KPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSAQLSGSVDIRLTKP
                     RTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKNADGEWKLAKEVRGNKAHVTDITLD
                     KPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMAKAAARSLGNNKVQ
                     VGFADVQAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQSGLLYYRTQLPGK
                     EISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLRVQYEGGTEDELIR
                     LTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEASPKTILGIEVSQEP
                     KKDYLVGDSLNLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQKTGRQTLTLRYQGH
                     EFNFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEAFLKAIEAAEQVLK
                     DHETSTQDQVNDRLNKLTEAHKALNGQEKFKEEKTELDRLTGEVQELLAAKPNHPSGS
                     ALAPLLEKNKALVEKVDLSPEELATAKQSLKDLVALLKEDKPAVFSDSKTGVEVHFSN
                     KEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVDLSYASIVKIPIEK
                     DKKVKKVFFLPEGKEAVELTFEQTDSHVIFTAPHFTHYAFVYESAEKPQPAKPAPQNK
                     VLPKPTYQPASDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRVIIQGRDGLLRHVF
                     EVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQNTAVKSEEASKQL
                     PNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            7098..8294
                     /locus_tag="VW854_001697"
     CDS             7098..8294
                     /locus_tag="VW854_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEV0262904.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            8430..8957
                     /locus_tag="VW854_001698"
     CDS             8430..8957
                     /locus_tag="VW854_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV0262905.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETILYVFVRVLETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            9034..9390
                     /gene="glnR"
                     /locus_tag="VW854_001699"
     CDS             9034..9390
                     /gene="glnR"
                     /locus_tag="VW854_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEV0262906.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            9427..10773
                     /gene="glnA"
                     /locus_tag="VW854_001700"
     CDS             9427..10773
                     /gene="glnA"
                     /locus_tag="VW854_001700"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEV0262907.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYVAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(10869..10988)
                     /locus_tag="VW854_001701"
     CDS             complement(10869..10988)
                     /locus_tag="VW854_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEV0262908.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            11246..11383
                     /locus_tag="VW854_001702"
     CDS             11246..11383
                     /locus_tag="VW854_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262909.1"
                     /translation="MNPDRAEEYFCRGCQGENPEDIEFYDEQLQAEKVEVLNIRLEVK
                     N"
     gene            11476..11991
                     /locus_tag="VW854_001703"
                     /pseudo
     CDS             11476..11991
                     /locus_tag="VW854_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187262.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         3857 a         2186 c         2568 g         3380 t
ORIGIN      
        1 aacaatgttt tagaaataga ggtgtactat tctagtttca atatactata tatctgcaga
       61 atactctttc agtttcattc agcctagtct tgcttaggaa aaagtaagaa gccatccaat
      121 tatgatggat agcttcttgg ttattagatt tgttctgcaa aaaccttttc agcaaggttc
      181 atagcgtggt ctgatacacg agtgtagtgt gagatgatgt cgataaagtt gaccccagct
      241 tgtgttgaac attcgccttt gttgaggcgt ttgatgtggg tttttctgag aacacgttct
      301 atcttattga ttgcttcatg acgttctaca agactgcgtg ctttttcaat atcattgttt
      361 tccacactat ccagagcatc tttgataaag tcactagttt ggcggtaaac ttcctctaat
      421 tctttcaagg cggcatcaga aaattcaaca tttttccgtt gaagatagtc agtcagattg
      481 agtagagcct ccgtgtggtc tccaatccgt tccaaatcac gggaggaatc aaggatattg
      541 gtgagcactt cactttcttt ttggctgaga gcttcgcttg aaagggcaat gagataacgt
      601 gttaattgct catcgatggt gttaattgct tcttcggttt tatgcccttt ttcagcaact
      661 ttttcatcca agtcaatgat gtacttatag gaaaggtcaa aggctttagc agcgtagttt
      721 cctaagtgca agagctcttt cttagcattt cctagagcga tagatggggc ctgtttgatg
      781 aaatgttcat caagatataa gggttcgtat tttacaacct cgtcctctcc aggaataatc
      841 ttggttacaa agtaagccag agctccgata aatggaaatt ggacaatggt gttggtaata
      901 ttaaaggttc cgtgagcaaa ggcgatggtc atttctggtg ctagatttag cgtagcttca
      961 aaccaatgaa tcaggacagt aaaaggaact agaaaaataa cgcagacgac tgttccgata
     1021 acgttgaagg caacatgagc tcctgctacc cgtttagctg caatattagc ccctaaagag
     1081 gcaatgatgg ctgtaatggt tgtcccgata ttgtcaccaa atagaactgg caaagctccc
     1141 tgtaggtcaa ttagattgcc ggcgtagagg ttttgtaaaa tcccaatggt agccgaagaa
     1201 gcttgaatta gcaaggtcaa gccggtaccg acaaagacac ccaaaacagg attcttactt
     1261 agctcaatca tatagtcctt aaagacctgt aaatccttga gtggagccat tgcgccgctc
     1321 atgagattga gggcaaaaaa gataccaccg acaccaaaga ggatgcgtcc gatattattg
     1381 actgtccgat tttttgtaaa aaagagacag acggcaccga taaagagcat aggtagggca
     1441 tagttaccta atttaaaacc gatgagaaag gatgtgacag ttgtcccaat attagcaccc
     1501 atgacaatcc cgatagcctg acgtaaggtt aagagaccgg cactgaccag gccgactgta
     1561 ataactgtta caccagaact agactgaatt agagcagtca tcccaatacc aaccagaact
     1621 ccaaagaaag gattactagt atatttgtca atgtaaaaac gaaggcgatc tccagcagct
     1681 tgttgcaaac cgtctcccat ggtcttgata ctatataaga atagccccag accacctaaa
     1741 aagtgaaata aaatttcctg ccaattaatg gacatttctt tttcctccga aaaataatcg
     1801 cggaatatct cctattctat tttaaaggat aaaagtaaat ctaacaagtg ttaatgtcaa
     1861 gtttgtaaaa gaaaattata attgataagt gtattatttt taagagtttt taaccttatt
     1921 ttagatttgt gaaaacagct attgtttttt gtctaaaaat gttttaggat aaaaatgtga
     1981 ttatcatggt tctagagttt gataaaaaac tacagaatat aaagcgcttt cttgaaaaca
     2041 acaaaatcaa tcttttagga ggagaaaatg aagaatccat tttttgaaag acgttgtcgt
     2101 tacagtattc gtaagttatc agtaggagcc tgctcgctga tgattggtgc tgttttattt
     2161 gttggtccag ccttggctga agaaactgca gttcctgaaa atagcggagc taatacagag
     2221 cttgtttcag gagagagtga gcattcgacc aatgaagctg ataagcagaa tgaaggggaa
     2281 catactagag aaaacaagct agaaaaggca gaaggagtag cgacagcatc tgaaactgca
     2341 gaagcagcta gcgcagctaa accagaggaa aaagcaagtg aggtggttgc agaaacacca
     2401 tctgcagaag caaaacctaa gtctgacaag gaaacagaag caaagcccga agcaactaac
     2461 caaggggatg agtctaagcc agcagccgaa gctaataaga ctgaaaaaga agtccagcca
     2521 gatgtcccta aaaatacaga aaaaacatta aaaccaaagg aaatcaaatt taattcttgg
     2581 gaagaattgt taaaatggga accaggtgct cgtgaagatg atgctattaa ccgcggatct
     2641 gttgtcctcg cttcacgtcg gacaggtcat ttagtcaatg aaaaagctag caaggaagca
     2701 aaagttcaag ccttatcaaa caccaattct aaagcaaaag accatgcttc tgttggtgga
     2761 gaagagttca aggcctatgc ttttgactat tggcaatatc tagattcaat ggtcttctgg
     2821 gaaggtctcg taccaactcc tgacgttatt gatgcaggtc accgtaacgg ggttcctgta
     2881 tacggtacac tcttcttcaa ctggtctaat agtattgcag atcaagaaag atttgctgaa
     2941 gctttgaagc aagacgcaga tggtagcttc ccaattgccc gtaaattggt agacatggcc
     3001 aagtattatg gctatgatgg ctatttcatc aaccaagaaa caactggaga tttggttaaa
     3061 cctcttggag aaaagatgcg ccagtttatg ctctatagca aggaatatgc tgctaaggta
     3121 aaccatccaa tcaagtattc ttggtacgat gccatgacct ataactatgg acgttaccat
     3181 caagatggtt tgggagaata caactaccaa ttcatgcaac cagaaggaga taaggttccg
     3241 gcagataact tctttgctaa ctttaactgg gataaggcta aaaatgatta cactattgca
     3301 actgccaact ggattggtcg taatccttat gatgtatttg caggtttgga attgcaacag
     3361 ggtggttcct acaagacaaa ggttaagtgg aatgacattt tagacgaaaa tgggaaattg
     3421 cgcctttctc ttggtttatt tgccccagat accattacaa gtttaggaaa aactggtgaa
     3481 gattatcata aaaatgaaga tatcttcttt acaggttatc aaggagaccc cactggccaa
     3541 aaaccaggtg acaaagattg gtatggtatt gctaacctag ttgcggaccg tacgccagcg
     3601 gtaggtaata cttttactac ttcttttaat acaggtcatg gtaaaaaatg gttcgtagat
     3661 ggtaaggttt ctaaggattc tgagtggaat tatcgttcag tatcaggtgt tcttccaaca
     3721 tggcgctggt ggcagacttc aacaggggaa aaacttcgtg cagaatatga ttttacagat
     3781 gcctataatg gcggaaattc ccttaaattc tctggtgatg tagccggtaa gacagatcag
     3841 gatgtgagac tttattctac taagttagaa gtaactgaga agaccaaact tcgtgttgcc
     3901 cacaagggag gaaaaggttc taaagtttat atggcattct ctacaacttc agactacaaa
     3961 ttcgatgatg cagatgcatg gaaagagcta accctttctg acaactggac aaatgaagaa
     4021 tttgatctca gctcactagc aggtaaaacc atctatgcag tcaaactatt tttcgagcat
     4081 gaaggtgctg taaaagatta tcagttcaac ctaggacaat taactatctc ggacaatcac
     4141 caagagccac aatcgccgac aagcttttct gtagtgaaac aatctcttaa aaatgcccaa
     4201 gaagcggaag cagttgtgca atttaaaggc aacaaggatg cagatttcta tgaagtttat
     4261 gaaaaagatg gagacagctg gaaattacta actggctcat cttctacaac tatttatcta
     4321 ccaaaagtta gccgctcagc aagtgctcag ggtacaactc aagaactgaa ggttgtagca
     4381 gtcggtaaaa atggagttcg ttcagaagct gcaaccacaa cctttgattg gggtatgact
     4441 gtaaaagata ccagcctacc aaaaccacta gctgaaaata tcgttccagg tgcaacagtt
     4501 attgatagta ctttccctaa gactgaaggt ggagaaggta ttgaaggtat gttgaacggt
     4561 accattacta gcttgtcaga taaatggtct tcagctcagt tgagtggtag tgtggatatt
     4621 cgtttgacca agccacgtac cgttgttaga tgggtcatgg atcatgcagg ggctggtggt
     4681 gagtctgtta acgatggctt gatgaacacc aaagactttg acctttatta taaaaatgca
     4741 gatggtgagt ggaagctagc taaggaagtc cgtggcaaca aagcacacgt gacagatatc
     4801 actcttgata aaccaatcac tgctcaagac tggcgcttga atgttgtcac ttctgacaat
     4861 ggaactccat ggaaggctat tcgtatctat aactggaaaa tgtatgaaaa gcttgatact
     4921 gagagtgtca atattccgat ggccaaggct gcagcccgtt ctctaggcaa taacaaggta
     4981 caagttggct ttgcagatgt acaggctgga gcaactatta ccgtttatga taatccaaat
     5041 tctcaaactc cgctcgcaac cttgaagagc gaagttggag gagacctagc aagtgcacca
     5101 ttggatttga caaatcaatc tggtcttctt tattatcgta cccagttgcc aggcaaggaa
     5161 attagtaatg tcctagcagt ttccgttcca aaagatgaca gaagaatcaa gtcagtcagc
     5221 ctagaaacag gacctaagaa aacaagctac gccgaagggg aggatttgga ccttagaggt
     5281 ggtgttcttc gagttcagta tgaaggagga actgaggacg aactcattcg cctaactcac
     5341 gcaggtgtat cagtatcagg ttttgatacg catcataagg gagaacagaa tcttactctc
     5401 caatatttgg gacaaccggt aaatgctaat ttgtcagtga ctgtcactgg ccaagacgaa
     5461 gcaagtccga aaactatttt gggaattgaa gtaagtcagg aaccgaaaaa agattaccta
     5521 gttggtgata gcttaaactt gtctgaagga cgctttgcag tggcttatag caatgacacc
     5581 atggaagaac attcctttac tgatgaggga gttgaaattt ctggttacga tgctcaaaag
     5641 actggtcgtc aaaccttgac gcttcgttac caaggtcatg aattcaattt tgatgttttg
     5701 gtatctccaa aagcagcatt gaacgatgag tacctcaaac aaaaattagc agaagttgaa
     5761 gctgctaaga acaaggtggt ctataacttt gcttcaccag aagtaaaaga agctttcttg
     5821 aaagcaattg aagcggccga acaagtgttg aaagaccatg aaactagcac ccaagatcaa
     5881 gtcaatgacc gacttaataa attgacagaa gctcataaag ctctgaatgg tcaagagaaa
     5941 tttaaggaag aaaagacaga acttgatcgc ttaacaggtg aggttcaaga actcttggct
     6001 gccaaaccaa accatccttc aggttctgcc ctagctccgc ttcttgagaa aaacaaggcc
     6061 ttggttgaaa aagtagattt gagtccagaa gagcttgcaa cagcgaaaca gagtctaaaa
     6121 gatctggttg ctttattgaa agaagacaag ccagcagtct tttctgatag taaaacaggt
     6181 gttgaagtac acttctcaaa taaagagaag actgtcatca agggtttgaa agtagagcgt
     6241 gttcaagcaa gtgctgaaga gaagaaatac tttgctggag aagatgctca tgtctttgaa
     6301 atagaaggtt tggatgaaaa aggtcaagat gttgatctct cttacgcttc tattgtgaaa
     6361 atcccaattg aaaaagataa gaaagttaag aaagtatttt tcttacctga aggcaaagag
     6421 gcagtagaat tgacttttga acaaacggat agtcatgtta tctttacagc accacacttt
     6481 actcattatg cctttgttta tgaatctgct gaaaaaccac aacctgctaa accagcacca
     6541 caaaacaaag tccttccaaa acctacttat caaccggctt ctgatcaaca aaaggctcct
     6601 aaattggaag ttcaagagga aaaggttgcc tttcatcgtc aagagcatga aaatgctgag
     6661 atgctagttg gggaacaacg agtcatcata cagggacgag atggactgtt aagacatgtc
     6721 tttgaagttg atgaaaacgg tcagcgtcgt cttcgttcaa cagaagtcat ccaagaagcg
     6781 attccagaaa ttgttgaaat tggaacaaaa gtaaaaacag taccagcagt agtagctaca
     6841 caggaaaaac cagctcaaaa tacagcagtt aaatcagaag aagcaagcaa acaattgcca
     6901 aatacaggaa cagctgatgc taatgaagcc ctaatagcag gcttagccag cctcggtctt
     6961 gctagtttag ccttgacctt gagacggaaa agagaagata aagattaaat atcgaaaaat
     7021 cttgtgaaat ctttccttat atttccaaag tgtgatataa tagtttcgaa taaaataaat
     7081 aaaggagttt ttgtaacatg gcaaaactta ctgttaaaga cgttgacttg aaaggtaaaa
     7141 aagtcctcgt tcgtgttgac ttcaacgtac cattgaaaga tggcgtaatc actaacgata
     7201 accgtatcac agcagctctt ccaactatta agtacatcat cgaacaaggt ggacgtgcaa
     7261 ttcttttctc tcaccttgga cgtgtgaaag aagaagctga taaagctggt aaatcacttg
     7321 ctcctgtagc agctgacttg gcagcaaaac ttggtcaaga tgttgttttc ccaggtgtca
     7381 ctcgtggtgc tgaattggaa gcggcaatca acgctcttga agatggacaa gttctcttgg
     7441 ttgaaaacac tcgttacgaa gatgttgacg gcaagaaaga atctaaaaac gatcctgaac
     7501 ttggtaaata ctgggcatca cttggagatg gtatcttcgt aaacgatgca ttcggtacag
     7561 ctcaccgtgc acacgcatct aacgttggta tctcagcaaa cgttgaaaaa gcagttgctg
     7621 gtttccttct tgaaaacgaa attgcctaca tccaagaagc agttgaaact ccagaacgtc
     7681 cattcgtggc tatccttggt ggttcaaaag tttcagacaa gatcggtgtt atcgaaaact
     7741 tgcttgaaaa agctgataaa gtccttatcg gtggtgggat gacttacaca ttctacaaag
     7801 cacaaggtat cgaaatcggt aactcacttg tagaagaaga caaattggat gttgcgaaag
     7861 ctcttcttga aaaagcaaat ggtaaattga tcttgccagt tgactcaaaa gaagctaacg
     7921 catttgctgg ttacactgaa gtgcgtgaca ctgaaggtga agcagtttct gaaggcttcc
     7981 ttggtcttga catcggtcca aaatctatcg ccaaatttga cgaagctttg actggtgcca
     8041 aaacagttgt atggaacgga cctatgggtg tatttgaaaa cccagatttc caagctggta
     8101 caatcggtgt gatggacgct atcgtgaaac aaccaggagt taaatcaatc atcggtggtg
     8161 gtgactcagc tgccgcagcg attaaccttg gccgtgcaga caagttctca tggattagta
     8221 cgggtggtgg agcatcaatg gaacttcttg aaggtaaggt tcttccagga cttgcagccc
     8281 tgacagaaaa ataagatttt ataaacaaat caaagaagag agggatgaaa gttcctcttt
     8341 tcttttgctt aaaataaaaa cgcttcctct caactattac tcataaaaat caccgattta
     8401 tgataaaatg gaaatagaaa gttgagatta tgagttattt taaaaaatat aaattcgata
     8461 aatcccagtt caaacttggt atgcgaacct ttaaaacagg tattgctgtt tttctagttc
     8521 tcttgatttt tggctttttt ggctggaaag gtcttcaaat tggtgctttg acagccgttt
     8581 ttagcctgag ggagagtttt gatgagagtg ttcattttgg gacttcgcgt attctaggaa
     8641 atagtatcgg tggactctat gccttggtct ttttcttatt aaataccttt ttccacgaag
     8701 ccttttgggt gaccttggta gttgttccaa tctgcaccat gttaaccatt atgacaaatg
     8761 tagccatgaa taacaaagca ggggttattg gtggtgtagc agctatgtta atcattaccc
     8821 tatcaattcc aagtggtgag acaattttgt acgtgtttgt gcgtgtatta gaaacgttta
     8881 tgggagtttt tgtcgcaatt atcgtaaatt acgatattga tcgtattcgt ctctttttag
     8941 agaaaaaaga aaaataatgt tacattttat aacattatca attgacgttt gtcttttttt
     9001 agactataat agacagaaag aaggaaattg taaatgaagg aaaaagaatt tcgccgaaat
     9061 atggctgttt ttcctatcgg cagtgttatg aagttgaccg atttatcggc gcgtcagatt
     9121 cgttattatg aagatcaaga gttgatcaag cccgatcgaa acgaaggaaa tcgtcgcatg
     9181 tattccttga atgacatgga tcgtctgctt gaaatcaaag attatatctc tgaaggttat
     9241 aatatcgctg ccattaagaa aaaatatgct gaacgtgaag cgaaatccaa gaaagcggtt
     9301 agtcagactg aagtacgtcg tgcacttcac aatgaactcc tccaacaggg gcgttttgct
     9361 tcagtacagt caccttttgg tcgcggttag gcaaccgcaa gtagtcatag attaaggaga
     9421 aaacttatgc caatcacagc tgcagatatt cgtcgtgaag tcaaggaaaa aaatgttacc
     9481 tttattcgtc ttatgttctc agatattttg ggaaccatga aaaacgtcga aattcctgct
     9541 acagatgaac agttagataa ggtcttgtcg aacaaggtta tgtttgatgg atcttctatt
     9601 gaaggttttg tacgtatcaa tgagtcggat atgtacttgt acccggactt ggatacatgg
     9661 acagtcttcc cttggggaga tgaaaatgga agtgttgcag gtctgatctg tgatgtctat
     9721 acaacagaag gtgaaccatt tgcgggtgac cctcgtggta atttgaaacg agctcttcgt
     9781 cacatggaag aagttggatt caaatccttc aaccttggtc cagagccaga attcttccta
     9841 tttaagttgg atgaaaatgg ggacccaaca cttgaagtga atgacaaggg tggctacttt
     9901 gacttggcac ctactgatct tgcggacaac acacgtcgtg agattgtgaa tgtcttgacc
     9961 aaaatgggat ttgaagtaga agcgagtcac cacgaggttg cggttggaca gcatgagatt
    10021 gactttaagt acgatgaagt tctccgtgct tgtgataaga ttcaaatctt taagcttgtt
    10081 gttaaaacca ttgctcgcaa acacggactt tacgcaacat ttatggcgaa gccaaaattt
    10141 ggtattgctg gatcaggtat gcactgtaat atgtccttgt ttgatgcaga aggaaataac
    10201 gccttctttg atccaaatga tccaaaagga atgcagttgt cagaaacagc ttaccatttc
    10261 ctaggcggtt tgatcaagca tgcttacaac tatactgcta tcatgaaccc aacagttaac
    10321 tcatacaaac gtttggttcc aggttatgaa gcgcctgttt acattgcttg ggctggtcgt
    10381 aaccgttcgc cacttgtgcg cgtacctgct tcacgtggta tgggaactcg tcttgagttg
    10441 cgttcagtgg atccaatggc gaacccttac gttgctatgg ctgttctttt ggaagttggt
    10501 ttgtatggta ttgaaaataa aatcgaagca ccagctccta tcgaagaaaa tatctacatc
    10561 atgacagcag aagagcgcaa ggaagctggt attacagacc ttccatcaac tcttcacaac
    10621 gctttgaaag ctttgacaga agatgaagtg gttaaagctg ctctcggaga tcacatctat
    10681 actagcttcc ttgaagccaa acgaatcgaa tgggcaagtt atgcaacctt cgtttcacaa
    10741 tgggaaattg ataattattt agacctttac taatactaat atagaagaaa gattgcctgt
    10801 gggtaaacag tcacacagca gtctaagcca tcaaatacct ataaccctta aaatagcgat
    10861 ttcggtcgct atttattttt cgaaaaatcc tcttgactct tttgcgaatt agatttatca
    10921 tcagacgtgt agaagaaaat taaggagtca ctatggaaaa attcaatttc aaaaacaata
    10981 tcgggcaaga aaacaagtta cttcaaatcg aaatctacaa gtttactaac ttttgcaagc
    11041 ttcaaaatta cacatctgta aatatttttt cgaaagatat atttgaagcc atagtaaatt
    11101 gagactagaa tagtacacta catctgctaa aaatttctag aaactaattt aactttccta
    11161 atcgatttgt ttacatctta tttcaatcta ctatattatc tcaataaatg atgatggcga
    11221 ctgttttgtt tgtgcttgtc gatttttgaa tcctgatcga gcggaagaat atttctgccg
    11281 aggttgccaa ggggaaaatc ctgaagatat tgaattttat gatgaacaat tacaggcaga
    11341 aaaggtggag gttttgaata ttcgattgga agtaaaaaat tgaattatct acaatctcat
    11401 caacaataaa cctatttcaa ccccattatc tataggcgta tttttacgcc tttttatata
    11461 cttgaagcac ataagaaaca ttatgggaag tttgctgatg gaagcactca agaaattgat
    11521 gttccttatg atattcctga tacttgggag tgggtgagga taaaatcaat ttattggaat
    11581 tttgggcaaa ataagccaga gaaatccttt aggtatatag atacgtctag tattgataga
    11641 aaaaagaata taatcaacta caaaaatcta caatatcttt cacctgaaca agcgccttcc
    11701 cgtgctagaa aattagtttc gcagaatagt gtcttatttt caacagttag accatatcta
    11761 aaaaatattg ctgtagttag agaacttaaa gagtatttga tagctagtac agcatttatt
    11821 gttttggata ctttacttaa cgaaacatat ttgaaatatt atttattatc agataatttt
    11881 atcaatcgtg ttaataataa atctactgga acaagttatc ctgcaatcaa tgattataat
    11941 tttaatctat tattaattgc tctcccccca ctatccgaac aacaacgaat a
//
LOCUS       DAVKWZ010000057        11827 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000057 DAVKWZ010000000
VERSION     DAVKWZ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11827
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.057"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(257..1243)
                     /locus_tag="VW854_001704"
     CDS             complement(257..1243)
                     /locus_tag="VW854_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV0262910.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            complement(1253..2053)
                     /locus_tag="VW854_001705"
     CDS             complement(1253..2053)
                     /locus_tag="VW854_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEV0262911.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTASQAPSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            complement(2306..3148)
                     /locus_tag="VW854_001706"
     CDS             complement(2306..3148)
                     /locus_tag="VW854_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV0262912.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(3150..4442)
                     /locus_tag="VW854_001707"
     CDS             complement(3150..4442)
                     /locus_tag="VW854_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV0262913.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            complement(4553..5824)
                     /locus_tag="VW854_001708"
     CDS             complement(4553..5824)
                     /locus_tag="VW854_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV0262914.1"
                     /translation="MSSKFMKSTAVLGTVTLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIVGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            6374..7891
                     /gene="malQ"
                     /locus_tag="VW854_001709"
     CDS             6374..7891
                     /gene="malQ"
                     /locus_tag="VW854_001709"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEV0262915.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVGQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEYFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            7917..10175
                     /gene="glgP"
                     /locus_tag="VW854_001710"
     CDS             7917..10175
                     /gene="glgP"
                     /locus_tag="VW854_001710"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEV0262916.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIELDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGDENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(11220..11792)
                     /locus_tag="VW854_001711"
     CDS             complement(11220..11792)
                     /locus_tag="VW854_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262917.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     FAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
BASE COUNT         3594 a         2212 c         2480 g         3541 t
ORIGIN      
        1 catctacaac ctcaaaacca tgttttgagc tgacttcgtc agtttcatct acaacctcaa
       61 aaccatgttt tgagcaacct gcggctagct tcctagtttg cactttgatt ttcattgagt
      121 ataaaaagat cttgggtaac aactcaattt cagtagaaaa ggaagtaaat cttcccacaa
      181 taaaacgcat agtatcaagt tttttcaata cctgatagta tgcgtttttg cttttaacga
      241 ctttttgccc agtctcttat ttttcgataa ttttgtgggc gatcaattga cggtaacaaa
      301 tttgtttatt gtttttatta tcattaataa tctggagaat agtttcaagg gaaacacgac
      361 caagctctaa actattgata tcgacatagg ctgccaagtt gagcttggga ttaaccgagt
      421 caaagctgag aacagggaca tccagctggt gtttggcaat atagttacaa acaccttcag
      481 ctaggaggct atcggttgtg atgatagcat caatttgtgg atcgtgcttg aataatcgct
      541 tgctaaattt atagcccttt tcttccagaa actcgtcggc aaagtagatg cgattgttgt
      601 cagtggtaag tttgtaatgt ttaagcgcct gttcatagcc tgttaaacgg tctttggtca
      661 cgaagagctt tttacttcct ccgataaagg caatgcgttt gcagcctttt ttgatgaaat
      721 attcagtcgc atcaaaacca gcttgaacat tgtcgttgtc gacaagtggg atgaaaggag
      781 atagagattt acctaagata aggaagggga actgttcttc tgcgacgagt tttacgagag
      841 ggtcttcttc ttgggcatag agaaagatta gcccatctac acgcttgccg tagaccattt
      901 gtgaaatagc gttgagacgc tccttctcat ctttccctgt tgctatctga atggcatagt
      961 ggttttcaga tgcgacttga gagatgccac gtagaaccga tggaaagaaa ggattctggt
     1021 agaaggcgtc tgagtcatca ggaagaacta atccgataac ctgagtatag ctgcttacca
     1081 agctacgagc gttgaggttt gggtggtagt tgagttcctt catagctttg cgaacgcgtt
     1141 tttttgtttc gtcgctaatg gttgatttat tttgaataac acgggttacg gttgaaggcg
     1201 aaacaccagc agccttggcc acgtctttaa tcgtaacggg cataaaaatc tcctatttat
     1261 ggtaatttgg atcacggaaa tgtgttttat aaaagatagt gaccagatac agaacaaaaa
     1321 gaatattttg tacagtaatc agggttgtca tattttggcc aaataatccc aaaataagtg
     1381 taatcagagt cggcaatcct aaacagttca agataaagtg atagcactct ttaaaggttc
     1441 taaatgaaaa gaggcgtgat ttcttagtga tataaaggag aagactagct cctagagaga
     1501 cgataaagaa attcaaacca aagaggaagc tcgcaccgag aactaggaag aggctgatat
     1561 agacacgatt ttgttggtac cagtctttag aaattgcttg ggtcaagctg tctttgcttt
     1621 tgaaactctc agtctgaatg gctcggtaag agatgcgggt cagttccttg ctttccttgc
     1681 tgatgactag ctcatttgta tcgaaatgca gttgcaagtc cttaggtaat tccttgattt
     1741 gacttggacc aatcacaaca gaaggggctt gactagctgt tccagtataa gttaatgtgc
     1801 catcgacaat tgtagcatgt tcagagagat cctggacaac ctcatttgtc agaggttcat
     1861 agacattatc gataaaagtt tctagcggat aggtctcctg ggagctgttt tggatagcaa
     1921 tgggtaccat agacaagctg ataaggaaga tactggtaaa gagaagttga aaccagttga
     1981 gctcgaaacg tttggacagg ggcttacgaa acccccaaat acttgaaaaa taggaaaatg
     2041 gatatggaag cataggtatc tttctaaaag gtgttttcta tatgagtatt attatataga
     2101 gaaatgcgct ttatttcaag tctaggagaa aaattgcttg ctacaaggat attatttaca
     2161 gtgacaattg ctaataatca atgaaaatca aagtgcaaac tagccacagg ttgctcaaag
     2221 cacaactttg aggttggaga taaagctgac gtggtttgaa gagattttcg aagagtataa
     2281 aatgaaaaag ggtggcgggg ataaattatc ccttgtcgcc accacttgta agtcctgaaa
     2341 caaagttctt ttgtaggaag aagaagagaa tacagattgg aagggcgatg aggatagcac
     2401 ctgctgagaa gtaggcaatc ttcaagtttt tcgcattgtt aacgaaggtt tggagaccta
     2461 cggcaacagt aaagtattct ttctcacgaa gcaagaaact agagaggatg tagtccccga
     2521 aaggtcccat gaaggcccag agagcttgta cggcaaccat tgggcgaaca agtggtagaa
     2581 caatttgcca gaagcggcgg aagtgtcctg caccgtctag ttttgcagat tcgtctaaag
     2641 acattggaac tgtatcgaag tagcctttca tgagccaagc attcatcggg ataccaccac
     2701 caacgtagag gaagatgagg aaccagttgt ggttaagggc gttcaacata agcgccataa
     2761 cgaagaaggc tgtcaaagcg gccatagttg gcaccatttg gatgatcaag aagaagacca
     2821 aactttgttt acgagccaag aagttgtaac ggctgtaagc ataaccagca agtacgatga
     2881 tacttgtttg aacagccatg gtaattaagg cgataatcaa agtgttgagg taccaagtac
     2941 cgtacaaggt ttcagtgaag aggcctttaa agttatcaaa attgaggtcg atattagtat
     3001 ctagtttaaa ggctgagacg ttacctgctt taaaggctga cataatggta atcaacagtg
     3061 gatagataat tacaattgat agaccaatca ggtaaaggta agtaaggctt tgagtcagtc
     3121 tacgtttgag tttaattgag ttattcatct tagacgtcct ccatatcaaa tgcgtgtagt
     3181 ttcttgaatg cgatcataga gattgagatg acaatgatag agataatcaa ggtaacagct
     3241 gccgccattg agtattgagg agatgtacct gttgtcaaac ggtagatcca tgagatcaag
     3301 atatcggttg aaccagctcc acctccgaca ctaccaggtc ctccaccatt gaagaggtac
     3361 atgatagaga agttgttaaa gttgaaggtg tattggctaa tcaaagtagg tgccgcaaca
     3421 gccaaaatca ttgggaaagt gatgttgcgg aatttttgcc aagcgttggc accgtcaata
     3481 taagctgctt cgtaaaggtc gttaggaata gattgcaaga tacccaaagt caaaacgtag
     3541 atgtatggga atccgagcca accttgcatc ataatcaagg caatcttagt ccaagttggg
     3601 tctgttttcc aaggaataag agctccatca aggaaaggaa ggaatttagc caagattggc
     3661 aatacttgag tgttgatagc accgacacta tcgttaaaca tgtttgagaa tgtcaagata
     3721 gtgatgaagg ctgggacagc ccaaggaaga aggaaaataa caccaaagat acgttttcct
     3781 ttgataaatg gttggttggc aatgatagct gtgaagatac caattacgat ttgtaaagta
     3841 gaagctgcca aagcccaaat gatagtccaa gaaagaacag aaccaaaggc agaacggaag
     3901 gtactcaagc tccaaatgtt tgtaaagttg gtcaaaccaa cccagtccaa caacttgttt
     3961 ggtggcaagt gttggaagtc gtagttggta aaggcgatca tcaaggttac gataactggg
     4021 aagataatcg cgaaggtcat ggcaacataa gatggaatga tcaagaggta agggaagcca
     4081 ttttcataaa tccctttgat catgtctttg agtgtgcgtg gaactggaat tccattgtta
     4141 atgcgttttg caatcgtatg tgcatctttg atatttgaga aataaaagag tacataaacg
     4201 attacaaaga ttagatggaa ggcaccacga atcagcataa agagggaatt atcacgacct
     4261 ggtttgtcac caagagtgat gaggttgctc aattcagggg ttgcaagtgc taggaagtaa
     4321 aggacaaata cgatggttac accaaggaag ataaaacctt tggctttttg tttattgtaa
     4381 atctgtccta acccaggaat gatagacagc agggctgctt tactaggttg ttgcttttcc
     4441 atattaactc ctttcatagg atactacttt tttataagaa agctaaatta tttgtgtctc
     4501 tattgataaa ttcaaagggg gatttgattt ccacccccct tgaacaaatt ttttattcac
     4561 caaatttttg tttgattgtt tctttgatca atgttacagc atcgttagca gctgttttag
     4621 catctttttg accacttaca gcatcaaaga gcatattttt cgctggatcc caaactgcag
     4681 acatttgaga gatgtttggc agtggttgag tgttcttgaa ctgtttgata acagctgttg
     4741 tcaactcatc gtttttacct tcagcgtatg aacgagcctc agtattagct gggatttcgt
     4801 tagtcttatc atataatact ttttgttgtt cagttgcaac aaggaagtct acaaattttt
     4861 gagaagcttc aaggttctta acggcttgag gaatgaccca agctttacca ccaccgaatg
     4921 cagcatattc ttttccattt ggaagagttg ggatagttgc aactccgtag tttactttag
     4981 catctttaaa ggcttgagct ttccaaggtc cgtcgatgat agcagctgtt ttaccttctt
     5041 ggaattgagt ttggattaag tttccagcac cttctgtatc ttgcatacct ttaggccatt
     5101 tttcgtacca agatttagcg tagttgatac ctacgataga accgtcgttt gcaagaccga
     5161 tgtctttagc gtctttaccg ttttggccaa agacgtaagc accgttaccg gcaagaagtc
     5221 catatgtata gtagaagttt gtccagtcag ctaggaaggc agtagtttta ccatcttcac
     5281 cagcgaatgc gtatttgcta tctttagcaa ggttttccaa gtcagcaaat gtttttggag
     5341 catctttcac caagtctttg ttgtagtaca taacaagtga ctcgataacg gcaggagcac
     5401 cgtaaacttt accattagca gctgttacaa gagatttagt tgtgtcgtct gttttagcac
     5461 catcgctcaa tttcacttct gaaagttgtc cgtcagaacc aaggctacct acacggtcgt
     5521 atggagccat cataacatca gggacattac cagattggtt gtcaagagaa agtttatcaa
     5581 gacctcctag agcatcacca gttttaagag tgacttttac tccagcttct ttttcataag
     5641 ctttagcaac ctcttcaata tagctcttat atccctcgtc tacatataca gtgagttctt
     5701 tgacttcaga tgaaccagaa tcagcaggct tatcagcagt tttgcttccg caagctacca
     5761 aaagcaagct agcaagtgta acagttccaa gcaccgcagt gctcttcata aatttagatg
     5821 acatagtgta ttcctcccaa agaatagcaa gttttattga taaggaaacg caaacgtttt
     5881 cctttatgag cttagtatag cacaaataga aaacggttgc aagtattttt tgtaaaaatt
     5941 ttaaaaaatt tttaagcttg atttgatagc ataattttgc attttaatag aagaaaagta
     6001 tgaaatagat aagaatacag tatttttaat aaaaatatat ggaatctgtc taaagaggga
     6061 aaccaggagg ggctacctcc ctgtttctaa tccctatcta ttttctatag acgtttgtgc
     6121 tttgttgcat ataaattcaa gctccctaag gaggttttgt actttgtaga ctgtctcgtg
     6181 agagtcaaac tttaggatta gatagtttta agttatcttt gctaaaatga gttagtctcc
     6241 ttaaaaataa tcaaaaaagt tttttaaaaa cgcttgcaat tatgcttgaa aaggagtata
     6301 cttataagta acgcaaacgt ttgcgtctgc aaaatacgca acgttccatt attttaacac
     6361 acgaggtgct attatgaaaa aacgtcaaag tggtgtgttg atgcacatct cttctcttcc
     6421 aggagcttac ggaatcggat catttggtca aagtgcttac gacttcgttg atttcttggt
     6481 ccgtacaaaa caacgttact ggcaaatcct tccattagga gcaactagtt acggggattc
     6541 tccttaccaa tctttctcag ccttcgcagg aaacactcat tttatcgatt tagatatctt
     6601 ggtggggcaa ggtttgttgg aagcaagtga ccttgaagga gttgactttg gtagcgatgc
     6661 gtctgaagtt gactatgcta aaatctacta tgcacgtcgt cctcttttag aaaaagcggt
     6721 gaaacgtttc tttgaagtcg gagatgttaa agattttgag aaatttgctc aagataacca
     6781 atcatggctt gagctctttg ctgagtatat ggctatcaaa gagtattttg acaatcttgc
     6841 ttggactgaa tggccagatg cagatgctcg tgctcgtaaa gcttcagcac ttgaaagcta
     6901 tcgtgagcaa ttggcagaca agttggttta ccaccgtgtg actcaatact tcttcttcca
     6961 acaatggttg aaattgaaag cttacgctaa cgacaaccac attgaaatcg ttggggacat
     7021 gccaatctac gtagcggaag attcaagtga tatgtgggca aatccacatc tcttcaaaac
     7081 agatgtcaat ggtaaggcta cttgtatcgc aggatgccca ccagatgagt tttctgtaac
     7141 tggtcagctt tggggtaacc caatctatga ctgggaagca atggacaaag acggctacaa
     7201 atggtggatt gaacgcttgc gtgaaagctt caaaatctac gatatcgttc gtatcgacca
     7261 cttccgtggc ttcgaatctt attgggaaat ccctgctggt tccgatacag cagcacctgg
     7321 tgagtgggtg aaaggtccag gctacaagct ttttgcagcc gttaaggaag aacttggtga
     7381 gctaaacatc atcgcagaag accttggctt catgacagat gaagtgatcg aattgcgtga
     7441 acgtactggc ttcccaggaa tgaagattct tcaatttgcc ttcaacccag aagacgaaag
     7501 cattgatagc ccacacttgg cacctgctaa ctcagttatg tacacaggaa cacacgataa
     7561 caatacggtt cttggttggt accgtaacga gattgatgat gcgactcgtg agtacatggc
     7621 tcgttacacg aaccgtaaag aatacgaaac agtggtacac gctatgcttc gtacagtatt
     7681 ttcatcagtt agctttatgg caattgcaac tatgcaagat ttactagaat tggatgaggc
     7741 agctcgtatg aacttcccat ctacccttgg tggaaactgg tcttggcgta tgactgaaga
     7801 tcaattgaca ccagctgtcg aggaaggttt gcttgacttg acaacaattt atcgccgaat
     7861 taatgaaaat ttggtagatt taaagaaata agacaataat caggagacaa ctaaacatgt
     7921 tatcactaca agaatttgta caaaatcgtt acaataaaac cattgcagaa tgtagcaatg
     7981 aagagcttta ccttgctctt cttaactaca gcaagcttgc aagcagccaa aaaccagtca
     8041 acactggtaa gaaaaaagtt tactacatct cagctgagtt cttgattggt aaactcttgt
     8101 caaacaactt gattaacctt ggtctttacg acgatgttaa aaaagaactt gcagctgcag
     8161 gtaaagactt gatcgaagtt gaagaagttg aattggaacc atctcttggt aatggtggtt
     8221 tgggacgttt ggctgcctgc tttatcgact caattgctac tcttggtttg aatggtgacg
     8281 gtgttggtct taactaccac tttggtcttt tccaacaagt tcttaaaaac aaccaacaag
     8341 aaacaattcc aaatgcatgg ttgacagagc aaaactggtt ggttcgctca agccgtagct
     8401 accaagtacc atttgcagat tttactttga catcaactct ttacgatatt gatgttactg
     8461 gttatgaaac agcgactaaa aaccgcttgc gtttgtttga cttggattca gttgattctt
     8521 ctattattaa agatggtatc aactttgaca agacagatat cgctcgcaac ttgactctct
     8581 tcctttaccc agatgatagt gaccgtcaag gtgaattgct ccgtatcttc caacaatact
     8641 tcatggtttc aaacggtgcg caattgatca tcgacgaagc aatcgaaaaa ggaagcaact
     8701 tgcatgacct tgctgactac gcagttgtcc aaatcaacga tactcaccca tcaatggtga
     8761 ttcctgaatt gattcgtctt ttgactgcac gtggtatcga gcttgacgaa gcaatctcaa
     8821 ttgttcgtag catgactgcc tacactaacc acacaatcct tgctgaagcg cttgaaaaat
     8881 ggcctcttga attcttgcaa gaagtggttc ctcacttggt accaatcatc gaagaattgg
     8941 accgtcgtgt gaaggcagag tacaaagatc cagctgttca aatcatcgat gagagcggac
     9001 gtgttcacat ggctcacatg gatatccact acggatacag tgttaacggg gttgcagcac
     9061 tccatactga aatcttgaaa aattctgagt tgaaagcctt ctacgacctt tacccagaaa
     9121 agttcaacaa caaaacaaac ggtatcactt tccgtcgttg gcttatgcat gctaacccaa
     9181 gattgtctca ctacttggat gagattcttg gagatggttg gcaccatgaa gcagatgagc
     9241 ttgaaaaact tttgtcttat gaagacaaag cagctgtcaa agaaaaattg gaaagcatca
     9301 aggctcacaa caaacgtaaa ttggctcgtc acttgaaaga acaccaaggt gtggaaatca
     9361 atccaaattc tatctttgat atccaaatca aacgtcttca cgagtacaaa cgccaacaaa
     9421 tgaacgcttt gtacgtaatc cacaaatacc ttgacatcaa agctggtaac atccctgctc
     9481 gcccaattac aatcttcttt ggtggtaaag cagctccagc ctacacaatc gctcaagata
     9541 tcattcactt gatcctttgc atgtcagaag ttattgctaa cgatccagca gtagctccac
     9601 acttgcaagt agttatggtt gaaaactaca acgttactgc agcaagcttc cttatcccag
     9661 catgtgatat ctcagaacaa atctcacttg cttctaaaga agcttcaggt actggtaaca
     9721 tgaaattcat gttgaacgga gctttgactc ttggtactat ggacggagct aacgtggaaa
     9781 tcgctgagtt ggttggtgac gaaaacatct acatctttgg tgaagattca gaaactgtta
     9841 tcgaccttta cgcaaaagca gcatataaat caagcgaatt ctacgctcgt gaagctatca
     9901 aaccattggt tgacttcatc gttagtgatg cagttcttgc agctggaaac aaagagcgct
     9961 tggaacgtct ttacaatgaa ttgatcaaca aagactggtt catgactctt cttgatttgg
    10021 aagactacat caaagtcaaa gagcaaatgc ttgctgacta cgaagaccgt gacgcatggt
    10081 tggataaagt catcgttaac atttctaaag caggattctt ctcatctgac cgtacaatcg
    10141 ctcagtataa tgaagacatc tggcacttga actaatactc ttcgaaaatc tcttcaaacc
    10201 acgtcagctt tatctgcaac ctcaaagcgg tgctttgagc aacctgcggc tagcttccta
    10261 gtttgctctt tgattttcat tgagtataag atacaaattt atactaatac attttgtaaa
    10321 aaagtgagtt tcgattgaaa ttcgcttttt taaatgctgt ggatttgggt caatcttgtc
    10381 taaaaatagg gaaatcctag atacagtgaa ggctttaaat gctggttttt actgtcttca
    10441 gccttatatt ttttcgtagt tggttacctc atatctcttg tattcgctta cataaagtat
    10501 tataatataa ttgtaggaaa gaaggtgttt ttatgatata cacacttaaa ttggtgttgt
    10561 ttattacctt tcttgtaata agcttgttac ctgataagat ttttggaaaa aataaaaaaa
    10621 tttggaaaat agtttttgca atattgacgg cagtggcagc attgtcattt atgtactaag
    10681 ttattttaag aatgtaggga aataaaccct acattctttt tagttttttc tgttttctaa
    10741 attctattta tccaagcgat tcaacatttc ttgcttcttc gcttcaagtt ctgcacgctt
    10801 ttcttcgatt tcggcatgtt ttttctcgag ttcagaacaa cttgcaccat tgctaaattc
    10861 ttttcgccat caggagatag ggtgagtcga catgtctatt actcacccaa agcagtccta
    10921 caaagcagga attttctgtt acttttttgg aaatagtaac gtttatacag ctttgacact
    10981 tcgtatcaaa gcgccaaaca cactccgagg gatttacaga aagcagaaaa ggaatgatct
    11041 ggtataagat cattcctttt ctctcttttt ctttaagtaa ttatatacaa tgtacgacga
    11101 agtcgtcatt gcaatgctga tccaccacct aaagggaact ttaaacaaca ttgataagat
    11161 aaagaatata aacaacgaaa atacgttata cccaattaat tttattgtat atctcatgat
    11221 taaaagttaa tccttccgtt gttaggaatg gcatcatttt tatcccataa ttgtgctaaa
    11281 taagtccccg gtgataataa attcatagcg aattctaaag caacatcatt tacaaaccaa
    11341 ctacctagat atctagaaat tgctgaacga atagcacttt ttgctgcatg ttttcctttt
    11401 actttaatta gatttgcaag gcctgcagta gttcctccta atgctaaagc tattgcagta
    11461 tctaatagag cacccatttg attaactgta ataccttgcc aagctgctct aaatggagag
    11521 tatgtaggtg ggattgtata atcgccttgt aattgtcggt taattacttc tttgatccat
    11581 tgttgtgaga cgtctggatg aaaagattgg atttcgtttg caagtgtatt gatttgttct
    11641 tctgttagag aagtgacagg ttgaagttcc atatttgttt caatttgtga tacttgttca
    11701 gaagcgtata cagctgaaac acttggaatc gctgatacaa ttaacacaat tgacgtcaaa
    11761 aaaaccgaaa taaatttcat taatttgttc atgagctttt ctccttttta tttgtatctg
    11821 cttacat
//
LOCUS       DAVKWZ010000058        11005 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000058 DAVKWZ010000000
VERSION     DAVKWZ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11005
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.058"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            1..971
                     /locus_tag="VW854_001712"
                     /pseudo
     CDS             1..971
                     /locus_tag="VW854_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1099..2121)
                     /gene="cbpF"
                     /locus_tag="VW854_001713"
     CDS             complement(1099..2121)
                     /gene="cbpF"
                     /locus_tag="VW854_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpF"
                     /protein_id="HEV0262918.1"
                     /translation="MKLLKKMMQVALATFFFGLLGTSTVFADDSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDPLSGEMVVGWQYIPAPHKGVTIGPSPRIEIALRPDWF
                     YFGQDGVLQEFVGKQVLEAKTATNTNKHHGEEYDSPAEKRVYYFEDQRSYHTLKTGWI
                     YEEGHWYYLQKDGGFDSRINRLTVGELARGWVKDSPLTYDEEKLKAAPWYYLDPTTGI
                     MQTGWQFLGNRWYYLHSSGAMATGWYQDGSTWYYLDAETGDMRTGWQYLGNKWYYLRS
                     SGAMATGWYQEGSTWYYLNASNGDMKTGWFQVNGNWYYTYDSGALAVNTTVGGYYLNY
                     NGEWVK"
     gene            complement(2140..2961)
                     /gene="cbpG"
                     /locus_tag="VW854_001714"
     CDS             complement(2140..2961)
                     /gene="cbpG"
                     /locus_tag="VW854_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpG"
                     /protein_id="HEV0262919.1"
                     /translation="MNVEGRGSANFIKDNVLITAAHNYYRHDYGKEADDIYVLPAVSP
                     SQELFGKIKVKEVCYLKEFRNLNSKDAREYDLALLILEEPIGAKLGTLGLPTSQKNLT
                     GITVTITGYPSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGYSGSTVYDASHRVVG
                     VHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGWKKINGSWYHYRQHDKQTGWQE
                     INDTWYYLDSSGKMLTDWQKVNGKWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            complement(3042..3281)
                     /locus_tag="VW854_001715"
     CDS             complement(3042..3281)
                     /locus_tag="VW854_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262920.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPESNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(3431..3697)
                     /locus_tag="VW854_001716"
                     /pseudo
     CDS             complement(3431..3697)
                     /locus_tag="VW854_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(3722..4033)
                     /locus_tag="VW854_001717"
     CDS             complement(3722..4033)
                     /locus_tag="VW854_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEV0262921.1"
                     /translation="MDWDFADTCWEKDPRKYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSLWDTVDILDRVVGSLVYEHPELEEIILILFENLFKPRQLYLQPQNSVLSNLRLA
                     S"
     gene            complement(3958..4200)
                     /locus_tag="VW854_001718"
     CDS             complement(3958..4200)
                     /locus_tag="VW854_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEV0262922.1"
                     /translation="MSLADLLEELEAAKDSKKARSMEAYMRHQFSFLGIAVPERNKLY
                     KNIFQKRKKQRLSIGILQTLAGKRILENTNMWLLTI"
     gene            complement(4201..4833)
                     /locus_tag="VW854_001719"
     CDS             complement(4201..4833)
                     /locus_tag="VW854_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0262923.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQEEF"
     gene            complement(4847..5842)
                     /locus_tag="VW854_001720"
     CDS             complement(4847..5842)
                     /locus_tag="VW854_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV0262924.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLYDVEKTEK
                     FVFLLLVFSMSMTCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVADPDLDASFKS
                     LSGKLNLLTEALQKAENQSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQIQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(5839..6537)
                     /locus_tag="VW854_001721"
     CDS             complement(5839..6537)
                     /locus_tag="VW854_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEV0262925.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAVTILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(6615..7625)
                     /locus_tag="VW854_001722"
     CDS             complement(6615..7625)
                     /locus_tag="VW854_001722"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEV0262926.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SSQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKAVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEANDQMK
                     KA"
     gene            complement(7609..8616)
                     /locus_tag="VW854_001723"
     CDS             complement(7609..8616)
                     /locus_tag="VW854_001723"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEV0262927.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLKICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYNLSVDQNLLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKAAAWLEEENLATVLERDWGFFISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKAEKVIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(8603..9556)
                     /gene="mvaD"
                     /locus_tag="VW854_001724"
     CDS             complement(8603..9556)
                     /gene="mvaD"
                     /locus_tag="VW854_001724"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEV0262928.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGHRYRLIVSKTKDLSQDDCC"
     gene            complement(9538..10416)
                     /gene="mvk"
                     /locus_tag="VW854_001725"
     CDS             complement(9538..10416)
                     /gene="mvk"
                     /locus_tag="VW854_001725"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEV0262929.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLNITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(10538..10717)
                     /locus_tag="VW854_001726"
     CDS             complement(10538..10717)
                     /locus_tag="VW854_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HEV0262930.1"
                     /translation="MLPHDFPLYLMVWSFFRRSMTTGWFQVNGRWYYAYSSGALAVNT
                     TVDGYFVNYNGEWVQ"
BASE COUNT         3278 a         2527 c         1956 g         3244 t
ORIGIN      
        1 gagaggacag aattagaacg tcttcaagca gaaaatgagt acctgagagc ggagaatgcc
       61 atcctaaaaa agttaagaga actccgattg aaggaggaaa aagagaaaga agaaagacag
      121 aaattgttca agaattaatg actgagtttt cgttagatct tcttctaaaa gccattaaac
      181 tagctcgttc gacctactac tatcacttga aacagctaga taaaccagat aaggaccaag
      241 agcttaaagc tgaaattcaa tccattttta tcgaacacaa gggaaattat gcttatcgtc
      301 gaattcattt agaactaaga aatcgtggtt atctggtaaa tcataaaaga gttcaaggct
      361 tgatgaaagt actcaattta caagctaaaa cgcgacagaa acgaaaatat tcttctcata
      421 aaggagacgt tggcaagaag gcagagaatc tcattcaagg ccaatttgaa ggctctaaaa
      481 caatggaaca gtgctacaca gatgtgactg aatttgccat tccagtaagt actcaaaagc
      541 tttacttatc accagtttta gatggcttta atagcgaaat tatcgcctat aatctttcaa
      601 cttcacccaa cttagaacaa gtacaaacaa tgttggaaca ggcattcaca gagaagcact
      661 acgagaatac gattctccat agtgaccaag gctggcaata tcaacacgat tcttatcatc
      721 gattcctaga gagtaaggga attcaagcat ctatgtcacg caagggaaac agccaagaca
      781 acggtatgat ggaatccttc tttgggattc tgaaatcgga aatgttttac ggttatgaga
      841 agtcgtttca gtcgtttaag caattggaac aagctattat agactatatt gattactaca
      901 acaataaacg aattaaggta aaactaaaag gacttagtcc tgtgcagtac agaactaaat
      961 cctttgaata aattatttgt ctaacttttt ggggtcagta cattgttaac ttgatactat
     1021 gcgttttttg attgtgaaga cttttatcca cctattatac aaagtcaagt atccatcaca
     1081 gtttacaatt agtcttcatt acttaaccca ttcaccatta tagtttaaat agtaaccacc
     1141 tactgtggta ttaacagcta aagcacctga atcataggta tagtaccagt taccattgac
     1201 ttggaaccag cctgttttca tatctccatt acttgcattt agatagtacc aagtcgaacc
     1261 ttcctgatac caaccagttg ccatagctcc tgatgaacgg agatagtacc acttgttacc
     1321 aaggtattgc cagccagttc tcatatcacc agtttcagca tctaaatagt accaagttga
     1381 accatcttga taccagccag ttgccatagc tcctgacgaa tggaggtagt accatctatt
     1441 acctagaaat tgccaaccag tttgcataat gccagttgtt ggatctagat agtaccatgg
     1501 agctgctttt agcttctctt catcatacgt aagaggggaa tccttaaccc aaccacgtgc
     1561 tagctctcca accgtcaatc tgttgatgcg cgaatcaaag ccaccatcct tctgtaaata
     1621 ataccaatga ccctcttcgt aaatccaacc agtttttaaa gtatgataac tacgctgatc
     1681 ttcaaaataa tagactcgtt tctctgctgg gctatcatat tcttccccat gatgtttgtt
     1741 ggtattcgta gcagtttttg cttctaaaac ttgcttgcca acaaattctt gtaagacacc
     1801 atcttgacca aaataaaacc aatctggtct aagagcaatc tctattcttg gagaaggacc
     1861 aatcgtaacc cccttgtgtg gagcaggtat atattgccag ccgacaacca tctctccgga
     1921 taaaggatca aaataatagt acttcccatc tatcactctc cagtaggttt cttttagatc
     1981 cccctttttg tagtaggttc taccattttc ttggacaaac tgccatcctt cagaatcatc
     2041 tgcaaatact gtactggtcc ctagcaaacc gaagaaaaat gtggctagtg cgacttgcat
     2101 catttttttc aaaagcttca ttttatatat cctccaacat taaatccact caccagatga
     2161 agcgaagtta taaactttac catcgatagt ttggctacct gtaaccattg ctccatttga
     2221 attgagataa taccattttc catttacttt ttgccaatct gtaagcatct taccggaact
     2281 gtctaaataa taccaagtat catttatctc ctgccaaccc gtttgtttat catgttgtct
     2341 ataatggtac caactaccat ttattttctt ccatccttca agagagtaac ctttaagaac
     2401 cgaataaata aatggcaaat ttcgttcatt taatttaact gcactgttaa tttgattagc
     2461 tccatctcct aaagtatgca ctcctactac acggtgacta gcatcataaa ctgtagatcc
     2521 actatacccc tctaaagtat caacttggta atccaagaac atgccatcat cacttaaaac
     2581 ttgtttttta tctgtataca tttgatgaat tttaaaatta tatgatggat agcctgtgat
     2641 agtcacagtt attcctgtca aatttttttg actagtagga agacccaaag tccctaattt
     2701 tgcaccaatg ggctcttcta gaattaataa agccaagtca tattcccttg catccttaga
     2761 atttaaattt ctaaattcct tcaaataaca aacttccttt actttgatct ttccaaatag
     2821 ttcttgactt ggactaacag ccggaagaac ataaatatca tccgcttctt tcccatagtc
     2881 atgtctgtag tagttgtgag ccgctgtaat taacacatta tctttaataa aattcgcact
     2941 tcccctccct tctacattca ttccatcggc tactccataa tacttagata aaaccatagc
     3001 tgaagtcgaa tacggatact gtaaagtatt atcaatttta atcaaatcat cattaccgat
     3061 aatacttctg attgcttttg gtagtatgaa ccatacgttg gtgaaatctc agataatgaa
     3121 gaatcattag actctggacc tttttctagt gtctcactta cctcatattc ttcacctgta
     3181 ctagaaataa cactcaaagc agatactgtc gataactggc tagccaataa agtactcgca
     3241 ataattgaaa tacccaattt tttataaaca gttttcttca ttatcgtatc ctcctaatgt
     3301 aattatagcg tactattcta aatttctcaa tctactatag aatcaagaaa tctaccacct
     3361 tctttaaata ccctccatta tcacataaac aggtaaactt ttcaattaat gactgcgctt
     3421 ttcaatcacg ctagaggtac ttgcttgctt ctttgatact aagttcagcc attctttcct
     3481 tgtttttctc aataaagcat gttacccaag tgggattcgt tttggagtag tctcgcagag
     3541 tccagccaat ggctttattg ataaaaaatt ctgtttggtt caagttatga aggagaatct
     3601 tttccattaa ttgagtattg gtcttctctt ttcttaacaa ctggtggtca atagcgacac
     3661 gtctcagcca gatattatct gataggctcc attttatact caatgaaaat caaagagcaa
     3721 actaggaagc tagccgcaga ttgctcaaaa cactgttttg aggttgcaga tagagctgac
     3781 gtggtttgaa gagattttcg aagagtatta agattatttc ttctagttca gggtgttcat
     3841 acaccaaact ccctactact cgatctagga tatctaccgt gtcccacaag gattttgtca
     3901 cgactaactg ctctagctta ggcaaatcgg tttcctttag ataagactgc attgctttca
     3961 aatagttagc agccacatat tggtattttc taggatcctt ttcccagcaa gtgtctgcaa
     4021 aatcccaatc gataatcttt gtttttttcg cttctggaaa atatttttat agagtttatt
     4081 tctttcaggc accgcaatac ctagaaaaga aaattgatgg cgcatatagg cttccatgga
     4141 ccttgctttt ttagagtctt ttgctgcttc tagctcctca agtaaatctg ctaaactcat
     4201 ctaaaactcc tcttgcccca ccaaatggtg ctgaaaggca tagacagccg cctgggtacg
     4261 atcgctgact tcaagtttgg caagaatatt ggacacgtgg gtcttgaccg tcttgagaga
     4321 gataaagagg tcatctgcga tgcgctgatt ttcgtagccc ttggcgatga gttggagaac
     4381 atctcgctca cgcgcagtca attcttcatg aagttccata tgattgcggt ggtattcaac
     4441 cttcttgcta acctcttgct caatggccag ctcgccagca gctaccttac tgacggcatg
     4501 aagcaattca tctgcactag aagtcttgag catatagcct ttggcaccag catctaagac
     4561 tggcatgatt ttttcattgt ccaaataaga ggtcacaatc aaaatcttgg cttcaggcca
     4621 ttctttaagg attgctaagg tcgcgtcaat tccattcatc tcaggcatga caatatccat
     4681 gacaatgaca tctggacgca gttccaaggc caagtcaatc ccttgagacc cgttggacgc
     4741 ctcacccaca acttctacat cgtcttggag gtcaaagtag cttttcaaac ccaaacggac
     4801 catttcatgg tcatctacta gtaaaatttt catctttact cctttatcat tccttatcta
     4861 acaggggaat acggatatca actgccagcc cttgcttggg agctgttaat aactgaaccg
     4921 tccctgccat atcttcaacc cgctccttga tatttcgcag tccataactc aagtcgtcta
     4981 agctccctaa ctggaaacca atcccattgt ccaccacctt cagttgcaat tcaacatctg
     5041 tctgatagag gtagacatct aggcaagatg cctgggcatg gcggagggta ttgctaatca
     5101 actcttgcag gatacggaag atatgctcct cgattttctt aggcaatttc gtcatattct
     5161 gcttgagact aaccctaaga tcactcttgt cctcaagctc ttttaagaga atctgaatcc
     5221 cttctatcaa gctcttctgc tccagttcaa ctggtcgcaa atgcaagagc aaaacccgca
     5281 aatccttctg ggcagtttct aaaatagctg tgacactctg caactggatc tgcatctttt
     5341 ctctatccaa tttcaaagcc tgctgactga tacccgataa aatcatgtgg gccgcaaaca
     5401 actcctgact gactgtatcg tgcaaatccc gagcaattcg cttccgttcc ttctcgatga
     5461 tttcctcttc ctgagcaagg ctctgatttt cagctttttg aagagcctct gtcaaaaggt
     5521 taagtttacc tgataaggac ttgaaactgg catccaaatc tggatctgca acctgaacca
     5581 cttcttgccc tgctaataaa cgcttgagat tagcctgcat ttttcttaga gaaagctctt
     5641 cgatccctcg ccaaaacagg gctaagagac aggtcatgga catgctgaaa accaacaata
     5701 aaaagacaaa tttttctgtt ttttcgacat cgtacaaaaa gatagaccag tcaaaatcaa
     5761 gtatttccag caagctgtgg gagaaaaaaa agacaaatag gaaggaggtg agagcaataa
     5821 tgacataggc ttgttttttc atcctctaac cacctccaca tcaccaatca tagtggtcaa
     5881 gaaaatcttg acactcttgt tactcttgag atagtctttt gtttcttgat gatagtgttc
     5941 attgcggagg gctcgcttgg gctggttgaa aaaagtcaaa tccccataga gacagttaac
     6001 gctgagactg acttccacat ctacaggtac gatgattttg gtcgttccta ccatctttct
     6061 gaggataatg acattgtcat gattggttaa gatgaccctc tccagatgaa tagtgtcctt
     6121 gcccatgaag cgaaagagat tgatatcatc gaattggcaa gtctggtagc ttgaaaaatg
     6181 atgaagattt ccaaaccaac gatttttctc cttcttaacc gtcacgacct cttcaaaaac
     6241 caaattggtc tgctcttttt cctggttcat catcgggtaa agaagaaaga ggctatagat
     6301 aaccgcaaca aaaatagcta gaatcacaaa aggattgagc ataacgatga aaaagaagag
     6361 aatggttacc gctactaaaa gaagattatt tccctcttta ccagtgtagt agcgaatcaa
     6421 aagcaaaaag aggaatagta tcagcagaaa acgcgaaaaa tgctctgata ccatcaaaat
     6481 cagagctcct gtcagaagac aggcttcgat aaataaaaag attttaaatt ttctcatagg
     6541 ttcatcctct cccttctatt ttatcacaat tcaaaaaagt cacctcagtc tgaggatgga
     6601 aaaaaaggcg ctggttacgc ctttttcatc tgatcatttg cttcttttaa ttttccataa
     6661 agaagatagt ctactttttg tagatctgct atggtggcac agttaaggga acacataatc
     6721 aagcgtagat ctgctttcca gccttggaca atgccaatca cttcttcaac tgtgtaggtt
     6781 tcaaccaatt ccagaacggt tcgtgacaat cccacagcct tagcaccaaa aaccaagcac
     6841 ttaatcatat ccagcggatt ccgaacccct ccactaacca agagttcgac cttatctttc
     6901 cattcttggg cattgagaag ggcctgcatg gtagactgac cccattgatt gaggtaatca
     6961 cgctggctac tacgacggtt ttcgatatag gcaaagctgg tgccaccacg acccgatagg
     7021 tccactgtac gaacaccgaa ttcataggct ctttcgattg tcttggcatc cattccaaag
     7081 cccacttcct tgaggacaat aggaacggga atttgcttgc tataatctgc tagatgcgat
     7141 tgccagcttc taaacttcct ttctccctcg ggcatgagta attcctgcat gacattgaca
     7201 tgcacttgca atagcacagg attcatctct tctacagtct gaagtcctaa ctcgacaggc
     7261 ttgtccaatc caatattggt tccaaggagg agattgggat gactagactt gacagaaaaa
     7321 gaatcatccg ttggattttt gagggctgcg ctataagaac ccgttacaaa taaaatacca
     7381 caggattccg ccacctgagc cagcttttga ttgatttctc ttcccttatt acttccacca
     7441 gtcatggcat tgatataaaa aggaaagtcc cactttcgac cagcaaactc tgtcgaaaga
     7501 tcgatttcat ccagattgta aagaggcaag gaagaatgaa tcagctccac ctcatcaaag
     7561 ctattatagg aacttttctg ctcaagggca tagaggatat gctcgtcctt acgatttgtc
     7621 gtcatgtcct atcctttctt gatataagag ctcaatcccc agatcggccc aacgattttt
     7681 taaggttttg gttgattgcg catcaaaact cagggcgatg ccacagtcac caccaccagc
     7741 accactactc ttggcaacgg cctgcaaatc ttgactggct tctttcaact gtctaagcaa
     7801 aggcgtgtaa atatctgtac tcaagccttc taaaagcttg ctggctactt ctacttgctc
     7861 gataactttt tcggctttcc cctgctccaa ggcttcgacc aaagaaacca ccgtttcttt
     7921 tgaggaactt aaaaaatttt gattgatatt ttgcttgatt tgctggacca tgtgactcga
     7981 tacagccact tccttggtcc atcccactaa gaaatcacat tctaaagttg gtttcacttg
     8041 tgagataaaa aatccccaat cacgctccag aactgtcgcc aagttttctt cttctaacca
     8101 agcagccgcc ttctggcgat caaatgactg gtagagaacc aaatcctctg ccacaataca
     8161 ggcaaggtcg cccatggaac cattgtctcc tcgcttgagc aagacagcgc tagtcagctt
     8221 gaacaagaga ttctgatcaa ccgaaagatt atagagagcc aataaagcct tgacaaccaa
     8281 gacaacgacg ctgccactag aacctagacc aaactttttc ccttctcgtt ccattttgcc
     8341 acagattttt agggaaaaag gtcttaaatt ctgaccgcga acagcgagga agtctcccat
     8401 caaagcaatc gtttcttgaa tcaagctgta gtcaggatta ggccttaagt ccactgcgaa
     8461 atcaaacata tctgaataga tacggtagct gtcagaaaaa gcaatctcag ccctcatata
     8521 gatgggaata tcctttatca aagctaactg ccctggctct aaaatagcat attcacctgc
     8581 ccaatagagt tttccgcaag ttttaacagc aatcatcttg actcaaatcc tttgtttttg
     8641 acacaatcaa gcgataacga tgaccgaaaa tttctgataa atgctccaag tctttctcct
     8701 gacagaagac cttaacattg ggaccagcat ccatggtaaa gtagcaggcc tctcctttct
     8761 cacgaagctg gcgaacaaag tccatagcct cataagaggc atccgtcaga taagaaaagg
     8821 ctggactagc agtctttgtc gtagcatgca tagccagggc atttttctcc gttaattctc
     8881 caatcttggc aaaatcattt tccttgagat aaatcagcat atcctgatag tccttctcag
     8941 actgacgaac ccagtcgtca aaagtcgtcg aggtttccac acaaagtttc atcccgtcac
     9001 ggctagagat tggttttttc ttgtcctcta gcaccaacat aatcatagct agtttcaagt
     9061 ctgtctctac agggtaaatt tctccactat ccttatccca ggctcctagt ggtccataaa
     9121 aactccgaga agaagaacct gaggcaaatt tggcttcctg tgccaactga cttctatcca
     9181 atccaagctt gaaataagca ttacaagcct tgaccagggc ggacaaacca ctagaacttg
     9241 aggacaggcc cgctgccgta ggcatattgt tttgagtatc gatacggaca aagccctcac
     9301 cagctggacg ataacggtca ataatcttac tcatcttggc atgctcgacc tcattttgta
     9361 gctgaccatt gatgtaaaat tcgtcagctg ttacattggc tggtaaaggc gacaaggtcg
     9421 tctctgtata catattttcc aaagttagag aaatactgct agtagcaggc accatctctt
     9481 tttctttttt ctttccccaa tatttgataa tagcaatatt tgcgtaggaa cgtactgtta
     9541 caggctctct atccatgtct gaacagctcc tttctcttct aatctttctg ctagttcttg
     9601 tgcgtgtgtc aaattggtta ccaaggctat gatacaacct cctagcccac caccgctcat
     9661 cttggcaccc agagcaccat ggctaagagt cgtttcaacc aaaaagtctg cctcagggct
     9721 actgactcca atttctttta aatgtaaatg cgcttgactg aggatttgtc ccagtccttc
     9781 agcatctttt tgtgaaatcg caacttctgc ttgctgggtt aattctccca aggcatgcaa
     9841 aaacggtagg gcatccttgc ccttattttg aaccacttgg atggcttcac gagtatgacc
     9901 ataaacaccc gtatcggcaa tcaccaaata ggcggataaa tccatctcaa gttctgtaaa
     9961 tcctacgttc ttgataaagc gaataggttg gtcactaaga caggtcttag catccaaacc
    10021 actaggattc atatgggcaa tcatttcagc tcgattgacc aagatttcta gtacatcatg
    10081 aggcagatca gcctgatagt agtcaaatac tgcacgaatg gccgctatgc tgatagccgc
    10141 tgacgaaccc atcccccgtt tctcagggat agccgagtca atctcacaac gaatgcaggc
    10201 ttctgtgata ttcaaatact ccagtgaggc ataaaccgcc atggacaagg tatcctcctc
    10261 ataaaggcgc caaggactct ctgcaggaac taccttacag gtcacctcca cctccaaaag
    10321 aggcagggaa atggcaggat aaccgtaaac gaccgcatgt tcccctatta aaattatctt
    10381 actatgtgcc tgaccgacac caactttttt tgtcattttt tccttttact agacgaaaaa
    10441 acgtcttatt tttcatacaa gtattaattc tttcctatct attttattat attttcacaa
    10501 aaaaagcgat tgtttccatt cacaatcgct tctttcatta ttgaacccat tcgccattat
    10561 aattgacaaa atagccatct acggtcgtat tcactgccaa ggcacctgag ctataagcgt
    10621 agtaccatct gccattgacc tggaaccaac ctgtcgtcat agaacgacga aagaaactcc
    10681 ataccattaa gtaaagagga aagtcgtgag ggagcatgcg ccattgacaa cctgttttag
    10741 tgacgtacaa agtctcatta acaagtactc gtttcggtca tttataggtg cggtgtttgg
    10801 agaaataggg ttcaatctta gcccattctt gatcgtttaa atcagtatca tatactttgc
    10861 gtgtcataac tctagtttaa cattttttgt gaatacaggt tctaaataat cgaccacgaa
    10921 aatttcttaa gtggaaaacg ccttatgaag tatgctacgg gaaagttatg cacttaattt
    10981 gacaattcaa gatgtaaaaa tatat
//
LOCUS       DAVKWZ010000059        10682 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000059 DAVKWZ010000000
VERSION     DAVKWZ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10682
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.059"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(92..1516)
                     /locus_tag="VW854_001727"
     CDS             complement(92..1516)
                     /locus_tag="VW854_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV0262931.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAIERAAYLHKTAAILERDKEEIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFAIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2059..4338)
                     /gene="pulA"
                     /locus_tag="VW854_001728"
     CDS             complement(2059..4338)
                     /gene="pulA"
                     /locus_tag="VW854_001728"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEV0262932.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKAYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGCLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4450..6408)
                     /gene="ligA"
                     /locus_tag="VW854_001729"
     CDS             complement(4450..6408)
                     /gene="ligA"
                     /locus_tag="VW854_001729"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEV0262933.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLSQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6501..7679)
                     /locus_tag="VW854_001730"
     CDS             complement(6501..7679)
                     /locus_tag="VW854_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV0262934.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7759..8622)
                     /locus_tag="VW854_001731"
     CDS             complement(7759..8622)
                     /locus_tag="VW854_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEV0262935.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTPLYFELNWILLQGLICQRDASYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8625..10526)
                     /locus_tag="VW854_001732"
     CDS             complement(8625..10526)
                     /locus_tag="VW854_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV0262936.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFNDLRRTERQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETIAWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLVTEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKSANMTFQSEKTSGNVVLTVENAAV
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSEQV"
BASE COUNT         3206 a         2367 c         1904 g         3205 t
ORIGIN      
        1 actataatac caaaaagttc gtattttttc tataattatt attttatagc aaaaaaataa
       61 aaccaggaaa acaatttcct gattttacac gttatttcac atcaaaaaca atggatttga
      121 catttgtcat cgcttcaatg ctatatttaa ttccttgcac tccagctcca gaacctttga
      181 caccaaggaa tgggaaatta tctggaccac gctgggtttt attattaatg tggactgtac
      241 ctacttcaag tttttcagca attgcaaagg cttttttgaa atcatttgta aaaactgatg
      301 attgaaggcc gaattcagat tcgttggcaa aagcaatagc ctcctctaca ctagccacac
      361 gaatgattgg taaaacagga ccaaatggct cttcccatgc cactttcata tcttttgtaa
      421 cttggtcaaa aagcactggc cagaggagat tgccctcacg tttgattggt gtaagagcct
      481 gagcctcttt ttcttgtgca tcctcaatca agccccaaat gaagtcggct gaagcattgt
      541 caataacagg tgtaatatca gcattgtcaa atggatcacc aactgttaat ttagaaactt
      601 cttcctgaag caaagtagct aatttatctg ctacactttc gagaacaatg acacgtttaa
      661 tagccgtgca acgttgtcct gagtagctaa aggctcccgc aacaatttgc ttggcagcat
      721 gttccaaatc tgcatcttct agtacaagag ctgcatcttt cccaccaagt tccaacatga
      781 taggacgcat accagctaaa cgaccaatac gttctccaat aggagttgaa cctgtaaagt
      841 tgatgaagtt gacttctttg tgctcaatga tataatcccc aatttctgaa ccacgacctg
      901 taatggtgtt gaaaacacct gccggaatcc ctgcttcttc aaatgcttta gccaacaaga
      961 gtccagaaat ggaaccttgt gttggtggct taaacatgac cacattccct gcaatcaagg
     1021 caggtgcaat tttagaagca gataaattaa ctggataatt aaagggagca atcgctagca
     1081 cgataccaac tggttcacga cggacaacag ccagtttgtt tttacttgct gcctcaaaac
     1141 caccaccttc cattgcttgt ccagtgatac ggagaccttc ctcagcagca taacgaatca
     1201 agtctgctgt acgcactact tctccaattg ctgctttaat cccttttgct acttctttgg
     1261 caaggatagt accaatttct tccttatcgc gttctaaaat agctgctgtt ttatgcaaat
     1321 aagccgcacg ttcaattgct gataaagctc gccatgctgg cagggctgca cgcgcagctt
     1381 gcatagcctc atcagcttca gtctgagtca tggctggaac tgtacccaat tcttcttgat
     1441 tgattggtga ataaatcgta atttcttgtt cagatgattt ccattttcca tttactaaat
     1501 tctgatatct tgtcaaattc ttgtcctcct gaaaatgatt aagatatatt ttaccaaatt
     1561 ttcagaaaac cacaaccctt tttgcgaaat ttttgagatt attttcacaa gcttgatttt
     1621 tcaaagtata ctcaataaaa ataaaaaaat ccactacgtc aaggcgaggc taatgtggtt
     1681 tgaagaaatt ttcgaagagc gtgaatgagt atcatctata gtaaaataaa aaactgaaca
     1741 atttggtttg ggacagtcaa accaatttct cacaatgttt tagagacaag ggtgtgctat
     1801 tccaatttca ggctactata actgtcatag attgctgaaa caaagtctag gtaaaagtct
     1861 tcataataaa aagacctcct atcaaatgtt caaaaacttt gataggaggt cttgtttcgt
     1921 gaaaatattt atcaaatttt ctatacaagt gagctgttag ccaggttctt tctattcttt
     1981 caatttcaat gaatggattt tttaccaata ctcataactg ggaatttttc tgtctaaaaa
     2041 tagcgagata gatggtattt ataaaacact caagacagct agactaatat catttaaaac
     2101 attatcttct tttgagcgac tgttggttac caacatagct aaatttcctg cattttcaaa
     2161 ttgatagggt tctgatttag cattcacaac taccaagagg tgttctttgc cgtgaacttc
     2221 atagataagg cagccgctat gttcaatcgc agaatgcaca aagacatgat ggtaaatttc
     2281 atcatagcta gagtaagaaa aggcaccagt ttttgtcttc aatcggatga cttgacggat
     2341 aaactcaata ctgtcttgac gctcattaat caagttccag ttcacttggt tcacactgtc
     2401 aggagcatta tagctattca tcgcacgctc tctatcatca tgggtcaact caccattttc
     2461 accagtcgca accagtttgg tacgaccaaa ttcttgaccg atttccataa aggccatccc
     2521 ctgcatgagc agattcatgg ctgtggcagt ttcgaccttg cgcatgattt gctctgaact
     2581 ttggtctgga tgaagggttg ccaataaatc gtgaagattg taattgtcat gggcttctac
     2641 atagttaagc acctgatttg gatgtgtata gcttcctaat tcacgactcc ctaggattgc
     2701 tttagctaga attggctctg tcgcagcacc actgacaaaa cctgacttga tagcaccata
     2761 aacttctccc cctttgacag catcgcgctg attgtcatta aagaaaccaa tatttggcat
     2821 ctggtaggca ttgtccttct tggccttatc ataaggggca agacctgttc ccatatccca
     2881 tccttctcca tagaggataa tgttggagtc gatttcatcc aagctttgac gaatcatctg
     2941 catggtcttg acatcatgaa tccccatcaa gtcaaaacgg aagccgtcaa tattatattc
     3001 ctgcacccag tatagaagag aatcaatcat atacttgcga aacatttcgt gttcactggc
     3061 tgtttcattt ccaacacccg ttccattctg gaaggtacca tctggattca tacgataata
     3121 gtaatcaggg actgttgttt ggaatggtgc atcaacaact gagaaggtat ggttatagac
     3181 tacatccata atgactccaa tacccgcatc gtgataagct tgaaccatca ccttcaaatc
     3241 acgaatgacc tgagctggat catctggatt agttgaaaaa ctagtttctg gcgcgttata
     3301 gttttgtgga tcataacccc agttgtaggt tacatttcca tcctcatcgt attctttatg
     3361 acggtctgca attggttgca attgaacata attgtagccc agcttcttga tgtaatcaaa
     3421 agcagttgac tggccgtatt ggttaactgt tccagcctga gcagcaccca agaaagttcc
     3481 tcgaagatgt tcatctacac ccgatgtagg tgatttagtc aaatcacgaa tatgcatttc
     3541 acagataact gccttacatg gattttccaa gcgccaagta gcctccgaac cgtgcttaac
     3601 ctcgaagttt tcaacttgct tttctacatg gctcagaata gctgaacgtt tgccatcagg
     3661 gctggtcgcg attgtataag gatcacgtgt cagtgtttgg tgatgaggga attggacttg
     3721 atactgataa gccttaccta ccaaatcttc ctcaacatcc aaactccaga caccgattgt
     3781 attgtcctta tgattataag agtagctatt gcctcttttc atctcaaaag tcttccaaac
     3841 gggtgcatca ttagcagctg attcataaac gacaacttgc acttctgtcg ctgtaggtga
     3901 ccagagggaa aaatgagcct gattgtcctc tacacggcaa cccaattctc cttggtaacc
     3961 ccaatgatga tcaaaactag cactgttaat ggccttatca aaggcaaaag gattttgatt
     4021 tttatagaaa ggactggcaa tagcaggatt ttcagagtaa taaatcctat catcgccttc
     4081 caaaatccag acctctgtta ataggggata gtgattaaaa cggatagaat attctttact
     4141 agtttgacct gtatgaacca caaaattcaa gctttctata acatgtgaac ttgggtgttc
     4201 aaagctaaat aaagctccaa aataatcttc tttgtaggtt agcaaatcaa ttcgttgatc
     4261 ctgacttttt acaaaggagc aagtgtcata ttctccattc ttacgatggt aatgaatgcg
     4321 catagggtag ttatacattt tttatttttc ctttttactt tgtttctatt tcactaataa
     4381 atttttgtca atctcgtctc aattaacaga catagtcata ttctctaaac tctgttttta
     4441 aacgatccat tacaaacttt ctagccatgc ctcatctctg acctggatac caagttcttg
     4501 tgctttttgc agtttacttc cagcgtctgc acctaccacg acgaggtcgg tctttttaga
     4561 aacactacct gtcactttgg cacccagact ttcgagttta cttttagctt ctgagcgctt
     4621 gagtcgttcc aattttcctg tcaataccac ggtcaaacct gacaaggccg catccgctac
     4681 taccgtctgt cctttatagt ccagattgac cccagtttct ttcaattctc tgagcagaat
     4741 ttcagagcct tctgtcgcaa aataagtctg aagacttttg gcaatcacgc cacctagact
     4801 ttcaatacta gccacttcct ctgaatctgc ctgagacaga ttttcaattg aatggaaata
     4861 ttgaagtaaa agctgactag ccttgcttcc gacatgacga attcccaaac caaataagag
     4921 cttctcggca gaattttcct ttgatgcttg gatagcctga tacagtttag cagcggactt
     4981 ttccttaacc ccctctaaaa ggaggaaatc ctcttcttgc aaacgataaa tatccgccac
     5041 atccttgact aaattagcag caaaaagctt ctcaacaata gatggaccaa ggcctgtaat
     5101 attcatagca tcacgagaag caaagtgaat caagccttcc atgatttgag cagggcaacg
     5161 cggattgata caacgtaggg ccacttcatc ttcaaagtgc aacaagtcag agttacaact
     5221 tggacagttt gtagggatat ctagtttttc ttcagaaacc cgtttggact ctaccacacg
     5281 taaaacggca gggatgatgt caccagcctt atatacaatg accgtatcgt cttttcggat
     5341 atctttttca gcaatataat ctacattgtg cagggtcgca cggctaacag tcgtaccggc
     5401 aagttgtact ggtgttagat tagcagttgg agttacaaca ccggtacggc caactgtcca
     5461 gtcaactgat aagagttgag cttctttttc ttcagcaggg aacttgtagg ctactgccca
     5521 ctttggagcc ttaacggtaa aaccaagttc ttcttgactt gctaggtcgt tgaccttgat
     5581 taccactcca tcaatatcgt aaggtagatt ttcccgttcc tgtcctactt cttggataaa
     5641 attccagatt tcatctatgt tttcagccaa gattcgctta ggattgacca caaaacctag
     5701 ttgttctagg tacttcaaac ccttttcttg gctatcacga gttgaagggc tggcttcttg
     5761 atagagaaac gttgcaagat tacgcttggc aactactgct gtatccaact gacgcagagt
     5821 tcctgctgcc gcattacgag gattagcaaa ttcaggctct ccattttctt ggcgcacttg
     5881 gttaacttgg tcaaaggaag cgcgtggcat gtaacattcc ccacgaactg tgatatctag
     5941 ttcttctggc aaagtcaaag ggatgtcctt aacacgcttg aggttttctg tgatattttc
     6001 accaattgaa ccatctccac gtgttacccc agcaaccaaa atcccctttt cataagtcag
     6061 cgagatagat aagccatcga ttttcagctc acaaatatag gtcggatgag ccacttcctt
     6121 acgaacacgc gcatcaaaag catctagctc ctcacgtgaa aaagcatcct gcaaactata
     6181 aagaggatac tgatgactgt atttttcaaa accatctaaa accttgccac caacacgatg
     6241 agtcggactg tctgctagca cttgctctgg ataagcagtt tctaactcga ccaactcacg
     6301 gtaaaggcgg tcatactcac tgtctgaaac cgagggatta tcgctggtat agtactcagt
     6361 cgcatagcga ttgagcaaag cgactaactc attcattctt ttattcataa gaccatttta
     6421 ccataaaaca agccctcctc acaaacgaga agggcggaaa aaacacttag tttgaaatta
     6481 tttttgaaac tcaagcaacc ttatatcaat ttttcaaaat gagttcgaac ataaataaac
     6541 gatatacaag acaagatgat aacaccactt ccaattatca ggaaagaaga gagatgtaca
     6601 cttggcaaga ctgtcataaa tccttttgca ataggcataa atagaatagc taaggtaaaa
     6661 attgtactca gtactctccc aagaaattcg ctctcaacct tggtttgtac ttgagtaaaa
     6721 aagtgaatat taaaaatcgt cataaacaat tcacaaacta aatttccaga aaaggaaaga
     6781 aaagttggaa gtggtaatcc catcataaaa actccgacac ctgtcaaagc cagtaaaatc
     6841 aaaagattat aaatattagc tttaatttta ctagctagaa gagccccaat gatggaacca
     6901 atagccccca tagttaaaat acttgcatag gctccttctg acccgtaaag ctgattcgaa
     6961 aagggaagta gaaattcaaa agctgcaaaa aagaaattaa cgctggaagc taccagcaaa
     7021 aggaagaaaa tttcttgctg atgccagata tagtgtaacc catccttgat atctacaaaa
     7081 atatctctcc cagtaaaagc ctttttctct tgaacttttg cttcctcttt tggaaggaaa
     7141 gccactagaa caaaagcaat gaaaaaagtc agcgagtcta gcagtagcgt catatggaga
     7201 cttgcaaact gtaaaacaag gaaggaaaga acaggagagc taacacctac aacctgcaaa
     7261 accagctcta agcgagaatt atagatcaca atctcatctt tctccaccac ttcagttatg
     7321 atagctttat tggctgtgcg agaaaaggca aaagcaatag cctgcacaat gttagcaaca
     7381 atcaaagcgc caatcatcca gctatcattc cttatgaaag aaatagccag acaaagaatc
     7441 ccacaaacaa gatctgccgt cattaaaatc ttacgacgag aaaaacggtc tgaaataact
     7501 ccgccaaagg gattgacgag aatagatgtg acgagctcag aaatctgata cattcctaaa
     7561 actgtctgtc ctatagtccc catagaagcc aaccagacac tatttccata atcatagagc
     7621 atatttccca ttttattgat agccccacgg ctaatcaact gcactgcata gcgattcata
     7681 ttaaagctcc tctcaaattt tgaaactatt gtatcaaaac cgaaaggagc tttttatttt
     7741 ttcccttatt tgggaaaatt aacttttgac aaatttttcg tagtgttcct gataataggc
     7801 tacttgctct ggaagaccta acacatcaaa aatatgcatg gcctcttgca tctgcttaca
     7861 gccttcttta cactgtcctt tttgatataa ggcaaaacct tttaaataat ggaaaacatt
     7921 acgctcataa agcttaatac ctttgtcaat aatcttctct gtataagcct caaaatagtt
     7981 ggcattataa aaagaagaat gctctaaaca atgctggtaa caattgaggg ccaaaatcaa
     8041 cactaatctc ttatggcgac taatctcttg gtaaaattcc tccctctcca taacttctct
     8101 accaatccga gtgacatagt ctacatcgta gaaactatag aggttaccga aaagaatcaa
     8161 ctcatacatg gtccattctt ctgttttgaa gagataatct gctaccttat ccaaatcatc
     8221 ctgcttcata tcataactcg catctctttg acaaatcaga ccttgtagca aaatccagtt
     8281 cagctcaaaa taaaggggag tcgtcgaact cttagacttt tcaagttgtt ctctttgaag
     8341 cttttgaaaa cctgcaatat cgtttgaata gtaaagtggg ataatctgtg ccatcataga
     8401 cacatgttca tgattatgaa aattccttgc cttatccatg aaattttcga ttgttacatg
     8461 aatgttatcc aaaatctcaa agaaacggga gactgccagg tcagactccc caagctcaaa
     8521 gcgagataac tgagaggtag agcaggattc gcctgctgct tcctttaaag aataatttcc
     8581 acttgttcga aattcacgaa atacttttcc aagatgttcc atctttacac ctgctctgat
     8641 aattcttccc actcaagcat agcttcttcc tgacgatggc tgattttgtc cagctcagcc
     8701 tgtaattcca tgagtttgtc ggcatcgttt gtttccaaca tttgttcaga aatggcttgg
     8761 ctttgacttt ctagctcttc aatttcagct tctagacttt cgatttgtcg catgagtttg
     8821 cgaacttctt tttgactttc tttctgggcc tgatagtcat tgactggact tgcttccttt
     8881 gcttgattgc tagttgaagc ttcctcagtc tgactcattt ctgctgttgc tttcttctca
     8941 acatagtagt cgtaatctcc aaggtagaga gttgaaccat tctcagacaa ttccaaaaca
     9001 tgagttgcca cacgattgat aaagtaacga tcatgactga caaacagcaa ggttccatca
     9061 aagtcaatca aggcattttc tagcacttcc ttactatcaa tatccaagtg gttggtcggc
     9121 tcatccagaa tcaaaaagtt attgttttcc atagacaatt tagctaaaag caaacgagct
     9181 ttttcgccac cagatagcat gccgactgat tttttaacat catctcctga gaaaaggaag
     9241 gctccaagac ggttgcggat ttcaacttct ggtgtcagtt tgaaatcatt ccagagttca
     9301 tccagcaccg tattacttgg tgtcagcttg ctttgggttt ggtcatagta accaacctca
     9361 acattagcgc caaagcgctt ttctcccttg ataaaaggaa tctggtccac aatagacttg
     9421 ataaaggttg acttgccgat accatttgga ccaacgatag cgacagcatt catcttacga
     9481 agatctaggt taatcggttg tgacaagact tccccgtcat agccaacagc tgcattttca
     9541 acagtcaaaa caacattgcc cgacgttttt tcagactgga aggtcatgtt ggctgatttc
     9601 ttgccagctt caggcttgtc caaacgttcc attttttcca gttgtttacg gcgagattga
     9661 gcacgtttag tcgttgaagc acgaactaga ttgcgattga caaagtcttc cagagcagcg
     9721 atttccttct gttgcttttc atagtttttt gcctcagtaa ctagcttttg ctccttcaat
     9781 tcgacaaaac gagagtaatt ccccacatag cgatccaagg aatgcttggt caaatctagc
     9841 gtaattgtcg caaccttgtc caagaaataa cggtcgtggc tgacgataat gagggcaccg
     9901 ctatagttta ccaagtaatt ctctagccag gcgatggttt caatatccaa gtggttagtt
     9961 ggctcgtcca agaccaagag attgggcttt tcaaggagca ttttggcaag tgccaaacga
    10021 gtattttgac caccagaaag ctcagcaatt ttcatctgcc acatagactc gtcaaacttg
    10081 aatccattca aaatcgctcg aatatcagct tcataggtaa agccacctgc ttggcgaaaa
    10141 ttctcagata agcggtcata atctgacatc agtttatcca aatcctcacc agacttttca
    10201 cccatctcca gctccatctg acgcagttgt ctctccgtcc gacgcaaatc attaaagaca
    10261 tgaagcattt catcgtagat ggtattttca gactcaaaac ggctatcttg ggctaggtaa
    10321 gacagagaaa tatctttttt cttattgatt tctccgctag ttggctcctc ttctccaact
    10381 aaaatcttca aaagagtaga cttacctgca ccatttttcc caacaagagc aatccgatct
    10441 cgttcatcaa cctgcaggtt gatattatcg aaaagaacct ctcctgcaaa agaacgttca
    10501 attttattag cttgtaaaat aatcatacaa gtagtatagc atgtttccct aaggcattca
    10561 agataatcgt aagtctttta gtacaacttt tataacataa aataaactaa attatgtata
    10621 ttttatatta gatttcttca ctatcttgtt ggattttcta accagctaat cttgtttcaa
    10681 at
//
LOCUS       DAVKWZ010000060        10603 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000060 DAVKWZ010000000
VERSION     DAVKWZ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10603
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.060"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            169..1956
                     /locus_tag="VW854_001733"
     CDS             169..1956
                     /locus_tag="VW854_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEV0262937.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPCEGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLNNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKEENL"
     gene            1953..2429
                     /locus_tag="VW854_001734"
     CDS             1953..2429
                     /locus_tag="VW854_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV0262938.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILGGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            2457..3362
                     /locus_tag="VW854_001735"
     CDS             2457..3362
                     /locus_tag="VW854_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV0262939.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            3349..4164
                     /locus_tag="VW854_001736"
     CDS             3349..4164
                     /locus_tag="VW854_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV0262940.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            4171..4575
                     /locus_tag="VW854_001737"
     CDS             4171..4575
                     /locus_tag="VW854_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV0262941.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            4874..6040
                     /locus_tag="VW854_001738"
     CDS             4874..6040
                     /locus_tag="VW854_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEV0262942.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCATDGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            6156..7193
                     /locus_tag="VW854_001739"
     CDS             6156..7193
                     /locus_tag="VW854_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEV0262943.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHVYNGTLLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            7400..8254
                     /locus_tag="VW854_001740"
     CDS             7400..8254
                     /locus_tag="VW854_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV0262944.1"
                     /translation="MTYEYKSHIYLAETALNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLENKFSVPGASWIAAGDYHHHLAVNEW
                     RGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSSKLEITDSDGI
                     VTRIRLAR"
     gene            8404..8661
                     /locus_tag="VW854_001741"
     CDS             8404..8661
                     /locus_tag="VW854_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEV0262945.1"
                     /translation="MTNSKYITCLKRSEGQLRGIQKMIEGDRDCADIVTQLTAVRSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
     gene            complement(8874..8957)
                     /locus_tag="VW854_001742"
                     /pseudo
     CDS             complement(8874..8957)
                     /locus_tag="VW854_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004238277.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl-tRNA synthetase"
     gene            complement(8991..9083)
                     /locus_tag="VW854_001743"
                     /pseudo
     CDS             complement(8991..9083)
                     /locus_tag="VW854_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(9119..9415)
                     /locus_tag="VW854_001744"
                     /pseudo
     CDS             complement(9119..9415)
                     /locus_tag="VW854_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            complement(9405..9566)
                     /locus_tag="VW854_001745"
     CDS             complement(9405..9566)
                     /locus_tag="VW854_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002875407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HEV0262946.1"
                     /translation="MDKKQAYIVSCHSGLRSYIAEPILKQAGFTVQNLDGAYSLYKMA
                     NPEGVEYGN"
     gene            9636..10400
                     /locus_tag="VW854_001746"
     CDS             9636..10400
                     /locus_tag="VW854_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEV0262947.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGANCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
BASE COUNT         2954 a         2002 c         2448 g         3199 t
ORIGIN      
        1 gagcgtttga aatgggtcta aaaatgtttg aaactaattg aatgtgtgta taatagtaaa
       61 aatgcagtaa atatcccgac aatttctatt gacaattcaa acagattggt ttataattaa
      121 tataacaaca aatgaaagcg caaactttcg cggtcggaag gtagttttat gacacgattt
      181 gagatacgag atgatttcta tctcgatgga aaatcattta agattttatc tggtgccatt
      241 cattatttta gggttcctcc agaggattgg tatcattcgc tctataactt gaaggctctt
      301 ggttttaata cggtagagac ttatgttgct tggaatttac acgagccttg tgaaggtgag
      361 tttcattttg aaggtgatct ggatttagag aaatttctcc aaatagcgca ggatttgggt
      421 ctctacgcaa ttgtgcgtcc gtctccattt atctgtgcgg aatgggaatt cggtggctta
      481 ccagcttggc tcttgaccaa gaacatgcga attcgctcat ccgacccagc atatatcgag
      541 gcagttggtc gctactatga tcagttattg ccaagactgg tgcctcgttt gttgaacaat
      601 ggtggcaata ttctcatgat gcaggttgaa aatgagtatg gttcttacgg agaagataag
      661 gcttacctga gagcgattcg acagctaatg gaagagtgtg gcgtaacctg tcccctcttt
      721 acatcagatg gtccatggcg agctactctg aaagctggaa ccttaattga agaggacctc
      781 tttgtaacag gaaactttgg ttctaaggca ccttacaact tttcgcagat gcaggaattc
      841 tttgatgaac atggtaagaa atggccactc atgtgtatgg agttctggga tggttggttc
      901 aatcgctgga aagaaccgat tatcacacgg gatcctaagg aattggcaga tgcagttcga
      961 gaggttttgg aacaaggctc tatcaatctt tacatgttcc acggtggtac aaactttggt
     1021 ttcatgaatg gttgctcagc tcgaggaact ttggacctgc cacaagttac atcttatgat
     1081 tacgatgccc ttctggatga agaaggaaat ccaactgcta aatatcttgc agtcaagaag
     1141 atgatggcaa cacatttttc agagtatccg cagttggaac cactctacaa agagagtatg
     1201 gagttggatg ctattccact agttgaaaaa gtttctttgt ttgaaacctt agatagcttg
     1261 tcaagtcctg tagaaagtct ctatcctcaa aagatggagg agctgggaca aagttatggc
     1321 tacctacttt atcgaacaga aacaaactgg gatgcagaag aagaaagact tcgtatcatt
     1381 gatggtcgag atagggccca gctgtatgtc gatggtcagt gggttaaaac tcaatatcag
     1441 acagagattg gggaagatat tttttatcaa ggtaaaaaga aagggctatc taggttagat
     1501 atcttgatag aaaatatggg gcgtgtcaac tatgggcata agttcttagc ggatacgcaa
     1561 cgtaagggaa ttcggacagg ggtctgtaag gatctgcatt tcttactaaa ctggaaacac
     1621 tatccactcc cactagacaa tcctgagaaa attgattttt caaaaggatg gactcaagga
     1681 caaccagcct tttacgctta tgactttaca gtcgaagagc caaaagatac ttacctagac
     1741 ttgtctgagt ttggtaaggg agttgccttt gtcaatgggc agaatctagg acgtttttgg
     1801 aacgttggcc caactctctc actttatatc cctcatagct atctcaagga aggtgccaac
     1861 cgcatcatta tctttgaaac agaaggtcaa tataaagaag agattcattt aactcgtaaa
     1921 cctacactaa aacatataaa ggaggaaaac ttatgacaat tgtaggatgc cgtattgatg
     1981 gacgtttgat ccacggacaa gtagccaatc tttgggctgg aaaactaaat gtttcacgca
     2041 ttatggttgt agacgacgaa gttgtcaaca acgatattga aaagagtggt ttgaaacttg
     2101 cgacaccacc aggtgtgaaa ttgagtattt tgccagttga gaaagctgca gccaatattc
     2161 ttggtggcaa atacgatagc caacgtctct ttatcgtggc tcgtaaacca gaccgcttcc
     2221 ttggtttggt agaagcaggt gtaccacttg aaacccttaa tgttgggaat atgtctcaaa
     2281 caccagaaac tcgttctatt acacgttcta tcaacgtagt agacaaggat gtggaagact
     2341 tccacaaact ggcagaaaaa ggtgttaaac ttactgctca gatggttcca aatgatccaa
     2401 tttcagactt tttgagctta ttaaaatagg aaaaaaattt ttaggaggtc attgttatga
     2461 tacaatggtg gcaaatttta cttctcactt tgtactcagc ttatcaaatc tgtgatgagt
     2521 tgacgatcgt ttcatctgca ggttcccctg tatttgctgg tttcattact ggtttaatca
     2581 tgggagatgt gactactggt ttacttatcg gtggtaactt gcaactgttc gttcttgggg
     2641 ttggtacctt cggtggtgct tctcgtatcg acgcaacttc tggtgcggtt cttgcgacag
     2701 ccttctctgt ttcacaagga attgatgcac cgcttgccat tactacaatc gctgtaccag
     2761 tagcagctct cttgacttac ttcgacgttc ttggtcgtat gactactacc ttcttcgctc
     2821 accgtgtgga tgctgcaatc gaacgctttg actataaagg tattgaacgc aactacttgc
     2881 ttggtgcgat tccgtgggct ctatctcgtg cccttccagt cttctttgcc cttgcttttg
     2941 gtggtgcctt tgtacaatca gtagtagact tcgttgaagc ctacaaatgg gttgcagatg
     3001 gcttgacact cgcaggacgt atgcttccag gtcttggatt tgcaatcttg cttcgttacc
     3061 ttccagttaa acgtaacctt cactaccttg ctatgggatt tggtttgaca gctatgttga
     3121 ctgttcttta ctcatatgta acaggtcttg gtggcgctgt tgctggtatc gtaggtactc
     3181 ttcctgctga agttgctgaa aaaattggtt tcgtgaacaa cttcaaaggt ttgtctatga
     3241 ttggtatttc tatcgtaggt attttccttg cagtgcttca cttcaaaaat agccaaaaag
     3301 tagctgtagc agcaccttct acaccatcag aaagtgggga aatcgaagat gacgaattct
     3361 aattacaaac ttacaaaaga agattttaat caaatcaaca aacgtagctt gtttactttc
     3421 caattaggtt ggaactacga acgtatgcaa gcttctggtt acctttacat gatcttgcct
     3481 cagttgcgta aaatgtatgg tgatggaact cctgaattga aagaaatgat gaaagttcat
     3541 actcaattct tcaatacttc accattcttc cataccatta tcgctggttt tgaccttgcc
     3601 atggaagaaa aagatggtgt aggttcaaaa gacgccgtta acggtatcaa gacaggtttg
     3661 atgggaccat tcgctcctct tggggataca atctttggtt cacttgtacc tgctatcatg
     3721 gggtcagtcg cagcaactat ggctatcgct ggccaacctt gggggatctt cctttggatt
     3781 gcagttgcag tagcgtatga catcttccgt tggaaacagt tggaatttgc ttacaaagaa
     3841 ggggttaacc ttatcaacaa catgcaaagt accttgacag ctttgattga cgctgcatcc
     3901 gtacttggtg tcttcatgat gggtgctctt gtagcaacag tgattaactt tgaaatttct
     3961 tacaagttgc caatcggtga aaagatgatt gatttccaag acatcttgaa ccaaatcttc
     4021 ccacgtttgc ttccagcaat ctttactgcc tttatcttct ggttgcttgg taagaaaggt
     4081 atgaactcta ctaaagctat cggtattatt atcgtacttg ctttggctct ttctgccctt
     4141 ggtcactttg cacttggaat gtaattcctt atgactaaat cattaatttt ggtgagccat
     4201 ggtcgcttct gtgaggagct tagaggtagc acagaaatga ttatgggccc acaagacaac
     4261 atttacacag tagctcttct tccagaagat ggcccagaag aatttactgc taagtttgaa
     4321 gctgttattg aaggattgga tgatttccta gtctttgcgg atcttctcgg tgggacacct
     4381 tgtaatgtgg tgagtcgctt gatcatggaa ggtcgtgata ttgaccttta cgcagggatg
     4441 aatcttccaa tggtgattga atttatcaat gcgagcctta caggcgcaga tgcggactac
     4501 aagagccgtg ctgcagaaag cattgtgaaa gttaatgacc tgttagcggg cttcgatgat
     4561 gacgaagatg aataatactc ttcgaaaatc tcttcaaact acgtcaacgt cgccttaccg
     4621 taggtatatg ttactgactt cgtcagtctt atccggcaac ctcaaaacgg tgttttgagc
     4681 tgacttcgtc agtcttatcc ggcaacctca aagcagtgct ttgagcagcc tgcggctagt
     4741 ttcctacaga ttttagttgg aactcgattc aattcatgtg acaacgtgaa aatcgttaga
     4801 gcattttata tagaatatac atgggaatgg agcttactcc cattcctata tttaatagaa
     4861 aaagaggaac tcaatgctac attatacaaa agaagacttg ctcgaattgg gtgcagaaat
     4921 cactacgcgt gaaatctacc aacagcctga tgtatggaga gaagcttttg aattttatca
     4981 agcaaaacgt gaagaaattg cagccttcct acaagaaatc gctgataaac atgactatat
     5041 taaggttatc ttgacaggtg ctgggacttc tgcttatgtg ggagacacct tgctacctta
     5101 ctttaaggaa gtctatgacg aacgcaaatg gaatttcaat gctattgcga caacagatat
     5161 cgttgccaat ccagcaacct atttgaaaaa agatgtggca actgtccttg tgtcttttgc
     5221 tcgtagtggg aactcgcctg aaagtgtggc gactgttgat ttggccaaat ccttggtgga
     5281 tgagctttat caagtgacga ttacttgtgc aacagatggt aaattggctc ttcaagctca
     5341 cggtgatgac cgtaatctct tgctcttgca accagctgtc tctaatgatg ctggatttgc
     5401 catgacttct agctttacgt ctatgatgtt gacagctctc ttggtctttg atcctacaga
     5461 atttgctgtt aagtctgaac gttttgaagt tgtatctagt cttgcccgta aagttttaga
     5521 caaggcagaa gatgtcaaag agctcgttga tttagacttt aaccgtgtca tctatctagg
     5581 cgctggtcct ttctttggac ttgctcatga agctcagctc aagattttgg aattaactgc
     5641 tggtcaagtt gcgaccatgt atgaaagccc agttggcttc cgtcacggtc caaaatctct
     5701 tatcaacgac aatacagttg ttttggtctt tggtacaacg acagactaca ctcgtaagta
     5761 cgacttggac ttggttcgtg aagttgctgg tgaccagatt gctcgtcgtg ttgtgctttt
     5821 gagtgatcaa gcttttggtc ttgaaaatgt caaagaagtg gcccttggtt gtggcggtgt
     5881 cttgaatgat atttaccgtg tcttccctta catcgtttat gcccaactct ttgccctatt
     5941 gacttcactt aaggtagaaa ataaaccaga tacaccgtct cctacaggta cagtaaaccg
     6001 tgtagtacaa ggtgtcataa ttcacgaata tcaaaagtaa gacagtgttt atgaattctt
     6061 gacaagagga tttgtaaatt atcagataaa ccatagattg tcagtacgct ttctatggtt
     6121 tgtttgcttg agagaaatag taaaaggaga acagaatgaa agcatacaca gagcgtgtat
     6181 ttggaaatgt tgagggcgag gatgtcttgg cctatcgatt tgagacagac ggtggctacc
     6241 aacttgaggt tatgacttat ggtgcgacca tcttgcgcta tgtcgcacct gacaaggctg
     6301 gaaattttgc caatgttatc ttgggatttg atgactttga tagttatgta ggcaatagtc
     6361 ccaagcatgg agcaagtgta ggtcctgtag cgggtcgtat tgcaggtgcg acctttgagc
     6421 tcaatggtaa gacctatgac cttgaggtta ataatgctag caactgtaat cacagtggtt
     6481 caactggttg ggattccagc ttgtttgaag ttgaagaagt aagcgatcat ggcttgactc
     6541 tctacacaga gcgtacagat gggacaggag ggttccctgg aaatctcaag atttggatca
     6601 gttatcactt ggaagaaact ggtgcctatg aaatcagcta caaggtgacg accgatcagg
     6661 atacgctggt caatccaacc aaccacagct atttcaactt gtctggtgat ttcacgcaga
     6721 cgattgaccg tcatgtcttc caactaaaca cagagggcat ttactcaatc gctcctgacg
     6781 gtgttcctgc caaaactcca gaagccaacc gtgatgtggt caaacacgtc tacaatggta
     6841 ccttgttgaa ggatatcttt gcagaagaag atgagcaaat ccagctggca tcaggtttgg
     6901 atcatccatt tgcccttcct gcaggccatg acaatgctgg attcctttat gaccaaaatt
     6961 caggtcgctt cctgcttttc aagacagaag ctccttgctt tgtggtctac acagcaaact
     7021 ttgtggatga aagtgtcatc ataggaggtc agccaatgct acagcacaat gggattgctc
     7081 ttgaagcgca agctttacca gatgccattc acagtgacct taaaggccaa gtcattctta
     7141 aagctggtca aaccttcacc agtaagacac gttatgaact tgttgtgaaa taaaaaagct
     7201 ggtcaaaaag tctttaaaat taaaataacg catattgtca ggtgatgaaa agtttgataa
     7261 tatgcgtttt attatgaaat gatttacttt atattatcct gaaatggtgt tatttttgag
     7321 ttatctggct tgactttctt gatggaaatt atataatagt tgtaataata ataattacaa
     7381 cgagaaaaga ggaccattta tgacctatga atataagagt cacatttatt tggcagagac
     7441 agctttaaat gtaaaggatt tggccagtca aacagccttt tatcagcaag ttattggttt
     7501 agaaatctta tctcaaactg agacagagtc cattctaggc cttggtggaa aagtcttggt
     7561 acagctgatt caagcacaag agagcggaga agtgagggaa cattatggtc tttaccattt
     7621 ggccattctc ttgcccacac gcaaggcttt ggctgatgtc ttgaaacacc tgacagattt
     7681 gcaaattcct cttgtcggcg gtgcagacca tggttacagt gaagctattt acttggaaga
     7741 tttggaggga aatggcattg aactctatcg agataagcca gtttccacat gggatattcg
     7801 agaagatgga cgcattatcg gagtgacgga ggctcttgcg gcgcaggaca tttatgagtt
     7861 gggggaaaga gtagagccct ttatcctggc agaaggtaca agaatggggc atattcatct
     7921 ttccgtcaag gatagtcgaa agtccagcca gttttatcaa aaggtgttag ggctagagaa
     7981 taaattcagt gtgcctggcg ctagttggat tgcggctgga gattaccatc atcatttagc
     8041 agttaacgaa tggagaggaa aaggtctggc ttcgcgtaag caaggcttac caggtttagc
     8101 ctactatgtc atcgaagtcg cacataaaaa agaactgtta acgattgccc aacgagcaca
     8161 agaagttgac gcaccaatca aatggatgac atcgagcaaa ttggaaatca cagactcaga
     8221 tggcatcgtg acccgtattc gtttagcaag gtaaaattag gaatttttaa taagttataa
     8281 gtattgctag tttgcttttt gaacggtctt tggtaacaga actgcgtgag ctggatgggt
     8341 gagaattttg acaaagaagc tgattagaga tataatgaag aaaaatcgag caaggaaaaa
     8401 gaaatgacaa attcaaagta tattacttgt ttgaaacgtt cagagggcca gttgcgtggg
     8461 attcaaaaga tgattgaagg agatcgtgac tgtgctgata ttgtgacgca actgacagca
     8521 gtgagatcta gtgtagagcg cgtgattgag atgataatta ccgaaaatct tactgaatgt
     8581 atcaatcagc cactagatga ttctgaagct caaaaggaac gcctagaaaa ggctatccga
     8641 tacttgatta aacggaaata aatgaatgtt ttgagacgat atgtgcaaag gtctattaac
     8701 tattagaata gtttaatgtg agtcaaaaga agttgtaaaa aatctttaaa attaaaaaac
     8761 gcatagtatc aggtattgaa atgtactgcc ccccaaaagt tagattcttt ctgtctaact
     8821 tttggggggc agtacataaa aaccttgata gtatgcgttt tattgtgggg agacttatac
     8881 tcttcgaaaa tctcttcaaa ccacgtcagt ttcaccttgc cgtagatatg gttactgact
     8941 tcgtcagttc tatccacaac ctcaaaacag tgttttgagc tgactttgtc agttccatcc
     9001 acaaccttaa aacagtgttt tgagcagcct gcggctaact tcctagtttg cactttaatt
     9061 ttcattgagt attacttcat cttctcatga aattgagttt ttgcccagct cctcttgatt
     9121 attctacttg accgggccaa gtattcatac caccttctac gttggtaacg gtgaggcctt
     9181 gggagctgag aaattggcag gtagatgcag agcggactcc tccttgacaa ataacatagt
     9241 attcatggtc aggtttgagt tctttgtagc cttgctctaa ggtacttaat ggaagatttt
     9301 tggcacctgg tgcatgtcct gcttggaatt catgtgcttc acggacatcg ataagttcta
     9361 gattttcatt ttagtatttt ccataaaagt cagccatgct gatactagtt tccatattct
     9421 actccttctg ggttagccat tttgtatagt gaataagcgc cgtcaaggtt ttggacggta
     9481 aatcctgctt gcttgaggat aggctctgcg atatagctgc gcaaaccact gtggcagctg
     9541 acgatgtagg cttgtttttt gtccagttca tcagcttcct ccttgacact ttgtcagttt
     9601 ttgatacaat agtacaaaat tagaggaggt gggctatgat tcagaaacat gcgattccca
     9661 ttttagagtt tgatgataat cctcaggcgg ttatcatgcc caatcacgag gggttggatt
     9721 tgcagttgcc aaagaagtgt gtctatgctt ttttaggtga ggagattgac cgctatgcga
     9781 gggaagtagg ggcgaactgt gttggcgaat tcgtttctgc taccaagacc tatccagtct
     9841 atgtcatcaa ctacaagggt gaggaggtct gtctggctca ggctcctgtt ggctccgctc
     9901 cagcagccca gtttatggat tggttgattg gctatggtgt ggagcagatt atctctactg
     9961 ggacctgtgg tgtcctagct gatatagagg aaaatgcctt tctagtccct gttcgcgctt
    10021 tgcgagatga gggagccagt taccactatg tggcaccttg tcgttatatg gaaatgcagc
    10081 cagaggctat tgctgctatt gaggaagttt tggaagacag agggattcct tatgaagaag
    10141 tcatgacctg gacgacagac ggtttttacc gagaaacggc tgaaaaggtg gcttatcgta
    10201 aggaagaagg ctgtgctgtt gtggagatgg agtgttctgc tcttgcggca gtagctcaat
    10261 tgcgtggggt tctctggggt gaattgttgt tcacagcaga ttctctagcg gacttggacc
    10321 agtacgacag tcgtgactgg ggctcggaag cttttaataa ggcgctagaa ctgagtttag
    10381 caagtgttca ccacctttag ttgtactggc aaaggatttg ttttatcata aaatgtctag
    10441 ctcatacttt tcaaaaatat gtttaaacga ggtcaccttc ctcttgtcct aggcatgttg
    10501 aggttgggaa aaatctttaa aatcagaaaa acgtatcata tcaggtgata aaaactttga
    10561 tactatgcgt tttatgttgg taagatttag agtgagatga aat
//
LOCUS       DAVKWZ010000061        10385 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000061 DAVKWZ010000000
VERSION     DAVKWZ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10385
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.061"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            8..202
                     /locus_tag="VW854_001747"
     CDS             8..202
                     /locus_tag="VW854_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV0262948.1"
                     /translation="MKGKEYENRTVSFAISSKGEKMKVENISYRVDHRILFDNISFDT
                     SSSDVTLITGKNGTGKSTLL"
     gene            complement(568..1878)
                     /locus_tag="VW854_001748"
     CDS             complement(568..1878)
                     /locus_tag="VW854_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEV0262949.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTSIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            2115..2987
                     /locus_tag="VW854_001749"
     CDS             2115..2987
                     /locus_tag="VW854_001749"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEV0262950.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
     gene            3117..3963
                     /locus_tag="VW854_001750"
                     /pseudo
     CDS             3117..3963
                     /locus_tag="VW854_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            4049..4999
                     /locus_tag="VW854_001751"
     CDS             4049..4999
                     /locus_tag="VW854_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV0262951.1"
                     /translation="MGKKRWARNGSESNDASYAQVVSLYNDTSISVSNNETDKVLTGS
                     LYTETNEQGLTIPSSLLKNWNEQTGKNLTTNDLIGKSVSASIVESAAETSKIAQFQTK
                     IVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTEELQK
                     NKKYTVLSQQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRSKEIG
                     VMKAVGYQNRGVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSITKVFE
                     LNLLSVLGTLVFALLLGYVSAYFPARKISKMDPVESLRYE"
     gene            5176..6183
                     /gene="gap"
                     /locus_tag="VW854_001752"
     CDS             5176..6183
                     /gene="gap"
                     /locus_tag="VW854_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEV0262952.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFIKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(6251..7087)
                     /locus_tag="VW854_001753"
     CDS             complement(6251..7087)
                     /locus_tag="VW854_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV0262953.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWNEIVNPGD
                     VCQLTFDEEDYSQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETHVSRLKQFSNKTSLAHCKLKTGRTHQIR
                     VHLLHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLERLTFTTFSNTFEKELKK
                     NG"
     gene            7213..9408
                     /locus_tag="VW854_001754"
     CDS             7213..9408
                     /locus_tag="VW854_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV0262954.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGVYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKSQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            9461..9613
                     /gene="rpmG"
                     /locus_tag="VW854_001755"
     CDS             9461..9613
                     /gene="rpmG"
                     /locus_tag="VW854_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV0262955.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            9623..9799
                     /gene="secE"
                     /locus_tag="VW854_001756"
     CDS             9623..9799
                     /gene="secE"
                     /locus_tag="VW854_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEV0262956.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            9854..>10385
                     /gene="nusG"
                     /locus_tag="VW854_001757"
     CDS             9854..>10385
                     /gene="nusG"
                     /locus_tag="VW854_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEV0262957.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAE"
BASE COUNT         3257 a         1788 c         2262 g         3078 t
ORIGIN      
        1 aatatagatg aaggggaaag agtatgaaaa cagaactgtt tcttttgcta ttagttcaaa
       61 aggagaaaaa atgaaagtag aaaatatttc gtatagggtg gatcatcgta tattgtttga
      121 taatatttct tttgatactt cgagttcaga cgtgacatta attactggta aaaatggtac
      181 aggaaagtca actttactat agtagattga aactagaata gtacacatct acttctaaaa
      241 tattgttaga aatcgatttg actatcctga tctatttgtc ctggtcttat ttcatttcac
      301 tatatttcaa attgagtatg acgaagtgcg ctcccatgtc ctgggaacgc actttcttca
      361 tatttttcat attcttgaat ccatcgataa agactattgg gatgagtttt taaagttgaa
      421 ctaatcattt ttacaggatg aggtttacag cagagagttt gaaggcttta tcaaaggttt
      481 ttcttggcat aatgactttt cctcgtttcc gcttaatttt gtgtctactt tatgatacca
      541 agtccaccct taagttagat aataaatcta acttaaggaa gctagaagga tgagaatcca
      601 ggtggtcaag agtcccaaac ttaagctgat agggacaccc agaataattt gctttttgaa
      661 ggcaagaccg cgttcctcta tattgggaag tgagagttga atgagagaac cagctgatga
      721 aaagggtgag atattagtag atagagcgcc aataacggtg gctgttgtga gtaagtgaat
      781 atcaatctga ggattttgag cactgatgat agcaatgatg ggaaagaggg ctggagctac
      841 aacggatagg gtggaactaa agagtgacat cactccggct atcacacaaa agaacagagg
      901 taaccagaaa tgaggaatgg atgttgtcat gaggtgccct atcagtgtga ctaaacctga
      961 cttgaccgct agagacatta gtaagctcat gccgcagagc atgataattg tagcccaggg
     1021 aactttagcc aaaatggctt cttgcttccc taatttgagc cttaaggcga ggcagaccat
     1081 gagtattgag acaaagccaa tatcaaatgt tttttgataa gtagctatcc aggcgatgtt
     1141 tgggaaaatg agatgcaaca agggaaaaag ccaaaccaaa accatgctgc tgatcatgag
     1201 caaggtggtt tgtctttgaa ccttgctgag gagtggtggt tggtcaatag tcaaggatga
     1261 gtttgttctt cccttactat agtgactgta acaggataat aaaagcaaga cgatgagtgg
     1321 gtagataatg ctgacgataa agatatgatt gccaagtgaa aaagcttgct cttcccatcc
     1381 catttgctta aacaggcctt gaaagacaat gcctgagcta ctggttatca aattagcccc
     1441 tcctgaagct ccccaattga cggcttgagc tccaatcaaa gggtgtttgt ccgctttttg
     1501 acagagggta atcgctagag gacagcaaac ggccatagta gtgaaaaatc cagcacctaa
     1561 agcagacaaa agggttgcca tcaggtataa aatcatgtag agggcgttag ggtgggtgcg
     1621 tgtgcggtag agaatgtgtt gagccaaaac atcaagagta ccgttagttg ttgcaacgtt
     1681 ataaaagaga gagacgctaa aaatggtaaa aaagagtgag gttggccaaa aatgaagaag
     1741 ttctttgggg cttaatccca tgagagtggt tgcgatgagg taagaaaaag caatagccag
     1801 caggccaata ttgattttgg tgcggtaacc aattccaatg gctagagcaa tggcgctaat
     1861 cattattaaa tgaatcattg aattgtcctt tcagttagaa tatggaaata ggttggcaat
     1921 tttggcgctc aaaagaccac ctgtttccta ttggtacttg actattatac acaaaaaaaa
     1981 tacaattgtc ttgacaattg tattgacact tgttagaatt aaaaatgagg tattcaggct
     2041 tgtgatttcc tatggaagtt aatagtaatt gcctctagtg cttacaagtg atattaaaaa
     2101 tagaggactt agtgatgtca atcatttcaa ctgatttaac cccttttcaa atagatgata
     2161 cattgaaagc agccttgcga gaagatgttc attccgaaga ttacagtacc aatgccattt
     2221 ttgatcatca tggccaagcc aaggtgtcgc tttttgccaa ggaagctggt gttttagcgg
     2281 gactaaccgt ttttcaaagg gtttttaccc tatttgatgc cgaggtgacc ttccagaatc
     2341 ctcatcaatt taaggatggg gatcgtttga ctagtggcga tttggtttta gaaatcatag
     2401 gctcggtgag aagtctctta acatgtgaac gcgttgcctt gaatttttta caacatttat
     2461 cagggattgc ttcgatgaca gctgcttatg tagaagcctt aggcgatgat tgcattaagg
     2521 tatttgatac tcgaaaaact actcctaatt tacgtctttt tgagaaatat gccgtgagag
     2581 ttggcggtgg ctataatcat cgctttaatt tatcagatgc tatcatgcta aaagacaatc
     2641 acattgcggc agtaggtagt gttcaaaagg caattgctca agcgcgtgcc tatgcccctt
     2701 ttgtgaaaat ggtcgaggtg gaagtggaaa gccttgctgc tgccgaagaa gctgcggcgg
     2761 cgggtgctga tattatcatg ttggataata tgtcattgga acagattgaa caggccatta
     2821 ccctaattgc aggacgttct cggattgaat gttctggaaa tattgatatg accactatta
     2881 gccgttttcg tggtttagcg attgattacg tctccagtgg tagtttaacc catagtgcta
     2941 agagtcttga tttttccatg aagggtttaa cctaccttga tgtctaagtt gtaaaataaa
     3001 ctaacttttt aaaggatgtc tttcctctag aacgagtttt atgtcagata gtttaaacgc
     3061 ctcttcaaat atagtaaaat gaaccaaaaa tagtacacaa tgtggtataa tcttttatgg
     3121 catattcaat agattttcgt aaaaaagttc tctcttattg tgagcgaaca ggtagtataa
     3181 cagaagcatc acacgttttc caaatctcac gtaataccat ttatggctgg ttaaagctaa
     3241 aagagaaaac aggagagcta aaccaccaag taaaaggaac aaaaccaaga aaggttgata
     3301 gagatagact taaaaactat cttactgaca atccagacgc ttatttgact gaaatagctt
     3361 ctgaatttgg ctgtcatcca actaccatcc actatgcgct caaagctatg ggctacactc
     3421 gaaaaaaaga accacaccta ctatgaacaa gacccagaaa aagtagcctt atttcttaaa
     3481 aattttaata gtttaaagca cctagcacct gtttagattg atgaaacagg attcgatact
     3541 tatttttatc gagaatatgg tcgctcatta aaaggtcagt taagaagagg taaagtatct
     3601 ggaagaagat atcagaggat ttctttggtt gcaggtctaa caaatggtga gttaatcgct
     3661 ccaatgactt acgaagagat gatgacgagc gacttttttg aagcttggtt tcagaagttt
     3721 ctcttaccaa cattaaccac accatcggtt attattatgg ataatgcaag attccataga
     3781 atgggtaagt tagaactttt atgcgaggag tttgggcata aacttttacc tcttcctccc
     3841 tactcacctg agtacaatcc tattgagaaa acatgggctc atatcaaaaa gcacctcaaa
     3901 aaggtattac caagttgcaa taccttttat gaggctcttt tgtcctgctc ttgtttcaat
     3961 tgactatagt tcacggatac agttgggaaa gaagctatta aatatttgga gagtcctttt
     4021 gggatgaaca tgtcaaatat tactatagat gggcaagaaa agatgggcaa gaaatggatc
     4081 tgagtcaaac gatgcttctt atgctcaagt agtgagtctt tataatgata caagtatctc
     4141 tgttagtaat aatgagacgg acaaagtgtt gactggatcc ctctatactg aaacaaatga
     4201 acagggatta acgattccaa gcagtttact aaaaaattgg aatgaacaga caggaaaaaa
     4261 tttgacaact aatgatctta ttggcaaatc agtctcagcc agcattgtag aaagtgctgc
     4321 cgaaactagt aagattgctc aatttcaaac gaagattgta cgtgtaatca atgatgaaga
     4381 tgacatggag gacagcaaca gtttcatgct gtctcatcaa atggaaacga ttttgaaaga
     4441 ggctggattt acgaaagctg tatcttattt tatcttggaa ctcaaagatc catcacagac
     4501 aaaagtagta acagaagaat tacagaaaaa taagaagtat actgtgcttt ctcaacagag
     4561 agttcttgat attgtgatta cctttattcg tgttattcag ggattattga ttgtgctttc
     4621 atcacaagct attgtggtag cagcggttat gattggtatc attatttaca tcaatatcat
     4681 gcaacgttcc aaggaaatag gtgtcatgaa agcagttggt tatcagaatc gtggtgtcaa
     4741 aggaattttt atttacgagg ctatctggat tgtaggcatc gccttgctgc tggcattttt
     4801 ggttgcacaa ggggtgggaa gtttggcgaa tgcgattgta agtcactttt acccatccat
     4861 cactaaggtt tttgaattaa atcttttatc tgttttagga actctagttt tcgctctatt
     4921 acttggttat gtctcagcct acttcccggc gcgtaagatt agtaaaatgg atcctgtaga
     4981 atcgttacgc tatgaataat aagggattcc ttgatttaca gtcttaaaaa gaaactcaca
     5041 tagaaaaaca atggccatac ttgcaattca actcaaatag ttgtataata ggtttgttga
     5101 aaggtgattt gtagtgattc aagttactct tttcacaata aaaatttcat gattttcata
     5161 aggaggaaat cactaatggt agttaaagtt ggtattaacg gtttcggacg tatcggtcgt
     5221 cttgctttcc gtcgtatcca aaacgtagaa ggtgttgaag ttacacgcat caacgacctt
     5281 acagatccag ttatgcttgc acacttgttg aaatacgaca caactcaagg tcgtttcgac
     5341 ggtactgttg aagttaaaga aggtggattt gaagttaacg gtaaattcat caaagtttct
     5401 gctgaacgtg atccagaaca aatcgactgg gctactgacg gtgtagaaat cgttcttgaa
     5461 gctactggtt tctttgctaa gaaagaagca gctgaaaaac accttaaagg tggagctaaa
     5521 aaagttgtta tcactgctcc tggtggaaac gacgttaaaa cagttgtatt caacactaac
     5581 cacgacgttc ttgacggtac tgaaacagtt atctcaggtg cttcatgtac tacaaactgc
     5641 ttggctccaa tggctaaagc tcttcaagac aactttggtg ttgttgaagg attgatgact
     5701 actatccacg cttacactgg tgaccaaatg atccttgacg gaccacaccg tggtggtgac
     5761 cttcgccgtg ctcgcgctgg tgctgcaaac atcgttccta actcaactgg tgctgcaaaa
     5821 gctatcggtc ttgtaatccc agaattgaat ggtaaacttg atggatctgc acaacgcgtt
     5881 ccaactccaa ctggatcagt tactgaattg gtagcagttc ttgaaaagaa cgttactgtt
     5941 gatgaagtga acgcagctat gaaagcagct tcaaacgaat catacggtta cacagaagat
     6001 ccaatcgtat cttcagatat cgtaggtatg tcttacggtt cattgtttga cgcaactcaa
     6061 actaaagttc ttgacgttga cggtaaacaa ttggttaaag ttgtatcatg gtacgacaac
     6121 gaaatgtcat acactgcaca acttgttcgt actcttgaat actttgcaaa gattgctaaa
     6181 taattcttga gttgatagaa agcaaggctt tgtggtcttg cttttttata tggaaaaatg
     6241 gatgacacga tcatccattc ttttttaatt ctttttcaaa tgtatttgaa aaggtagtga
     6301 aagttagcct ctctaaagta agtgggtggg taaaggaaag tcggaaggca tgaagcataa
     6361 gccggcttgt ctttgattta ctattataga gagggtctcc caggatagga agattatgat
     6421 gcaaaaggtg cacacgaatc tgatgggttc gccctgtctt tagcttgcaa tgagccaagg
     6481 aagtcttgtt tgagaattgc tttaatctgc ttacatgcgt ttcagcatat tgcccatttt
     6541 ttgcatcaac tattcttttt ctacgatcat ggcgatcacg tccaattttg tctctgaaaa
     6601 caagttcttt tctgttgata tttccatcaa ctagagccca atattctcta gaaatctctt
     6661 ttttctccaa taagcgattg agaatgggca ggataaaagg atttttggca aagagaacta
     6721 agccactggt ttccatgtcc agacgatgaa cgacatagca ggtttggcca acataagtac
     6781 tgacatggtt aagaagggca atttcgtttg gttgattacc atgtgttttc atcccctctg
     6841 gtttgtttac aataatcaag tgttgatctt gataaacttc ctgcactaag tctgggttgc
     6901 cccaagggat cgttttttgg gaataatctt cctcgtcaaa agtcaactgg caaacatctc
     6961 caggatttac gatttcgttc cagtggactt cttcttgatt tatcaaaata tgttttttga
     7021 ttctcaaaaa atgacggatt tttctaggga tgaggagttg ttcctcaagt aattgcttta
     7081 ccgtcatttg aggtagagag gcgggtaatg taaatgtgaa ttgcatacag atattgtaac
     7141 aaaaaaagcc ctatttggat aggaaatagc taaattcttg tcttcctatg atgaagatga
     7201 taaaataaac gcatgaaatt agataaatta tttgagaaat ttctttctct ttttaaaaaa
     7261 gaaacaagtg aactagagga ctctgattct actatcttac gtcgctctcg tagtgatcga
     7321 aaaaaattag cccaagtagg tccgattcga aaattctggc gtcgttatca tctaacaaag
     7381 attatcctta tactaggttt gagtgcaggc ttgctagttg gagtctattt gtttgctgta
     7441 gccaagtcga ccaatgtcaa tgatttgcaa aatgccttga aaactcggac tcttattttt
     7501 gaccgtgaag aaaaagaggc tggtgccttg tctggtcaaa agggaaccta tgttgagctg
     7561 actgacatca gtaaaaactt gcagaatgct gttattgcga cagaagaccg ttctttctat
     7621 aaaaatgacg ggattaacta tggccgtttc ttcttggcta ttgtcactgc tggacgttca
     7681 ggtggtggct ctaccattac ccaacagctg gctaaaaacg cctatttatc gcaggatcaa
     7741 actgttgaga gaaaagcgaa agaatttttc cttgccttag aattaagcaa aaaatatagt
     7801 aaggagcaaa ttctaaccat gtaccttaac aacgcttatt ttggaaatgg tgtgtggggt
     7861 gtagaagatg cgagtaagaa atactttgga gtttctgcat cagaagtgag tctggatcaa
     7921 gctgcgactc tggcagggat gctcaagggg ccggaactgt ataatccctt gaattctgta
     7981 gaagattcta ctaatcggcg cgatactgtc ttgcagaata tggttgcagc aggatatatt
     8041 gataaaagcc aagaaaccga agctgctgaa gttgatatga cttcgcaatt gcacgataag
     8101 tatgaaggaa aaatctcaga ttaccgttac ccctcttatt ttgatgcggt ggttaatgaa
     8161 gctgtttcca agtataatct aacagaggaa gagattgtca ataatggcta ccgcatttac
     8221 acagagctgg accaaaacta ccaagcaaat atgcagattg tttatgaaaa cacatcgcta
     8281 tttccgaggg cagaggatgg aacgtttgct caatcaggaa gtgtagctct cgaaccgaaa
     8341 acagggggag ttcgtggagt tgtcggtcaa gttgctgaca atgataaaac tggattccgg
     8401 aatttcaact atgcaaccca atcaaagcgt agtcctggtt ctacaattaa gcctttagtt
     8461 gtttatacgc cagcagttga agcaggctgg gctttgaata agcagttgga taaccatacc
     8521 atgcagtatg acagctataa ggttgataac tatgcaggga tcaaaacgag tcgagaagtt
     8581 cctatgtatc aagccttggc agaatcgctt aatctacctg ctgttgccac tgttaatgat
     8641 ttgggtgtcg acaaggcttt tgaggcaggc gaaaaattcg gactcaacat ggaaaaggtc
     8701 gaccgtgttc ttggtgtcgc cttgggaagc ggtgttgaaa ccaaccctct tcaaatggct
     8761 caagcatacg ctgcctttgc aaatgaaggt ttaatgcctg aagctcattt tattagtaga
     8821 attgaaaatg ctagtggaca agttattgcg agtcataaaa attcacaaaa acgggtgatt
     8881 gataagtctg tagctgacaa gatgaccagt atgatgttgg ggactttcac caacggtacc
     8941 ggtattagtt catcgcctgc agactatatt atggcaggga aaactggaac aactgaagca
     9001 gttttcaatc cggagtacac aagtgaccag tgggtaattg gttatactcc ggatgtagtg
     9061 attagccact ggcttggctt tcctaccact gatgaaaatc actatctagc tggctctact
     9121 tcaaacggtg cagctcatgt ctttagaaac attgccaata ccattttacc ttatacgcca
     9181 ggaagtacct ttacggttga aaatgcttat aagcaaaatg gaattgcacc agccaataca
     9241 aaaagacaag tacaaaccaa tgataatagc cagacagatg ataatttgtc tgatattcga
     9301 gggcgtgcgc aaagtctagt agatgaggct agccgggcta tctcagatgc gaagattaag
     9361 gaaaaggctc aaacaatatg ggattcgata gtcaatctat ttcgttaaga tgcttgtcaa
     9421 agcctagctt tcttgttata atggataaga tggaggcgtt atggcactaa aaaaagcaag
     9481 cctagcttgt gcggtttgtg gttcgagaaa ctattcaatc aagatcagcg gaaaccccaa
     9541 gcctacacga ctagaagtaa ataaattttg taagcattgt ggcaagtaca ctacacacag
     9601 agaaacgaga taggagagag cgatgcgttt tattggagat atttttagac ttcttaaaga
     9661 cacaacatgg ccaactcgca aggaaagctg gagagatttt cgttctatca tggaatacac
     9721 agctttcttt gtagtaatta tttacatttt tgaccagttg attgtttcag gtttgattcg
     9781 atttattaac attttttaga agattagtgg agttaattac actagaaatc ttctatttat
     9841 gaaaggaaat atcatggata gttttgataa aggatggttt gttttacaaa cttattctgg
     9901 ttatgaaaat aaggtgaaag aaaatctatt acaacgtgca caaacctaca atatgttgga
     9961 taatattcta cgcgttgaaa ttccaacaca aacagtgcaa gttgaaaaaa atggaaagag
    10021 aaaagaagta gaagaaaatc gctttccagg ttatgttctt gtagaaatgg tcatgacaga
    10081 tgaagcttgg tttgttgttc gaaacacacc aaatgttaca ggatttgtcg gatcacacgg
    10141 gaacagatca aaaccaactc cattattgga acaagaaatt cgtgacattt tggtatctat
    10201 gggacaaact gttcaagaat ttgatttcga tgttgagatt ggtcaaactg tacgtattat
    10261 tgatggtgct tttgcagact acactggtaa gattacagaa attgataata acaaagtgaa
    10321 aatgattatc tctatgtttg gtaatgacac agttgcagaa gtaaacctaa accaaatcgc
    10381 agaat
//
LOCUS       DAVKWZ010000062        10215 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000062 DAVKWZ010000000
VERSION     DAVKWZ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10215
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.062"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            <1..76
                     /locus_tag="VW854_001758"
     CDS             <1..76
                     /locus_tag="VW854_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEV0262958.1"
                     /translation="IKIKLPIDSRGTPDWNYMERYRDR"
     gene            42..356
                     /locus_tag="VW854_001759"
     CDS             42..356
                     /locus_tag="VW854_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262959.1"
                     /translation="MGITWKDIEIDKEREGEIDKKSTPIFYGEFKNVRLSKEEYKNLK
                     EKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWYEEDKDKLRQKGLNKKMNYDVGE
                     SL"
     gene            1040..1201
                     /locus_tag="VW854_001760"
     CDS             1040..1201
                     /locus_tag="VW854_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262960.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
     gene            1293..2972
                     /locus_tag="VW854_001761"
     CDS             1293..2972
                     /locus_tag="VW854_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV0262961.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLGAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDYGVNLICVEDGIDSFKDAGKLMISVLSAVAEIERENIRIQTMEGCIQKAREGKWNG
                     GFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAI
                     VSDEVWQAAQVKLKSQAKKYERVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKD
                     GTKYKDFYYYGCKHRQMIRGHECTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEK
                     INMKVDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLCR
                     MYDKIDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVEHRQLISAL
                     ISEIQVYEEKQSNGQWLKSITFKLPIIEENLNIGLDNDEQVECVSLLEKRS"
     gene            3240..3806
                     /locus_tag="VW854_001762"
     CDS             3240..3806
                     /locus_tag="VW854_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262962.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            4128..4316
                     /locus_tag="VW854_001763"
     CDS             4128..4316
                     /locus_tag="VW854_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEV0262963.1"
                     /translation="MATDKNRIMISLDDKKLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            4378..5136
                     /locus_tag="VW854_001764"
     CDS             4378..5136
                     /locus_tag="VW854_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV0262964.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVYDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVIF"
     gene            5194..5655
                     /locus_tag="VW854_001765"
     CDS             5194..5655
                     /locus_tag="VW854_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEV0262965.1"
                     /translation="MLGVIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGIWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRF"
     gene            5689..6048
                     /locus_tag="VW854_001766"
     CDS             5689..6048
                     /locus_tag="VW854_001766"
                     /inference="COORDINATES: protein motif:HMM:NF015692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEV0262966.1"
                     /translation="MNDNPIECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQET
                     VPQAIIAFLESRDFEDAIRNVISLGGDSDTLAAITCSIAEAAYGIPDWIKDKAYSYLD
                     EPLKDVVRRWENRIKVY"
     gene            6160..7044
                     /locus_tag="VW854_001767"
     CDS             6160..7044
                     /locus_tag="VW854_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEV0262967.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            7207..7911
                     /locus_tag="VW854_001768"
     CDS             7207..7911
                     /locus_tag="VW854_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HEV0262968.1"
                     /translation="MELSAIYHRPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDP
                     FIFMEEFYQDTKEMVKITSGTLFDHWQVEVSVDFARIQYLFELRDTEGQNILYGDKGC
                     VENSLENLHAIGNGFKLPYLHEIDACKVPDWVSNTVWYQIFPERFANGNALLNPEGTL
                     DWDSSVTPKSDDFFGGDLQGIIDHMDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEI
                     DRHFGDSVAWVRQGIF"
     gene            7887..8342
                     /locus_tag="VW854_001769"
     CDS             7887..8342
                     /locus_tag="VW854_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0262969.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGGGNLQP
                     EKWNETAKQLYNIIMWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKRVNTTIKN
                     GSKGKTLTEMINGYRADNDIRDEICNFNILKNKIRDMKDQQGNTMESYF"
     gene            8787..9263
                     /locus_tag="VW854_001770"
     CDS             8787..9263
                     /locus_tag="VW854_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0262970.1"
                     /translation="MIGKNIKSLRKTHDLTQPEFARIIGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNPWILMSDDLSDLIQTNIYLVENFDEIERYSGYLDGIERMLEISEKRMVA"
     gene            9263..10024
                     /locus_tag="VW854_001771"
     CDS             9263..10024
                     /locus_tag="VW854_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PezT"
                     /protein_id="HEV0262971.1"
                     /translation="MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQEYGKDSVEYTKDFAGKMVESLV
                     TKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
                     LYIINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRSCVYDSKENTT
                     SAADVLQELLFGEWSQVEKEMLQVGEKRLNELLEK"
BASE COUNT         3714 a         1360 c         2102 g         3039 t
ORIGIN      
        1 tatcaaaatt aaattaccga tagacagtag aggtactcca gattggaatt acatggaaag
       61 atatagagat agataaagag agagagggag agatagataa aaagtccacc cccatttttt
      121 atggagaatt caaaaatgta aggttaagca aagaagaata taaaaatcta aaagaaaaat
      181 taaactcaca cacagatata atgataaata aactatccag atacatggaa agcagtggta
      241 agacctatca aaaccactat gtgacaatct taaaatggta tgaagaagat aaagacaaac
      301 taagacagaa aggtttaaat aaaaaaatga attatgatgt aggagaatct ttatagataa
      361 aaagaggtgg aaaggcacta ttaaagactt taagtctaaa aaggtatcaa agtatgccag
      421 agataaaaaa atataaatat aacactttaa aggcgattag aaaattaaag aaatgctaga
      481 agacttttag atgacagtta ccaagagggt ctaattttca ctgctactta tgcagtggga
      541 atttgacctt tttatataag caaattctat ttcaaaattg gttaccaaga aagacttctt
      601 ttcactgtta agtataagag ggtaatttat agaattttgt tacattaaga taaatatttt
      661 ctgatggtca aattttaaaa agattaataa ctctcgataa attttgaacc ttaacaattg
      721 aatagaccaa gacaagacgt taattatttt tggagcgtaa taacctatcc gtcaagatct
      781 ttcggctgaa taattcggca aaaaaagttc tgctaagtaa aaagaaagtg agcgataagt
      841 aagagtttga atatagggag ggatacccta tccgctatga agaaaatgag gataatgata
      901 cttctaaggt tgaagccggc tcatccggaa tagaggcgga ggtgagattc ctatgttgct
      961 aatccaaagc acttgtcaat gtcaaaaaac ttaattaaac aaataattat tagatataaa
     1021 actttagagg aggtatatat tggacaatga ctggaatgga ttggctgatt taatagcaaa
     1081 tctgattgca aaatatgctg gtgctttaga tttagataat ctacccgatc caacgccagc
     1141 aaaaaatcaa gagatgaaaa atagttttga catggcaaaa acacaaattg agacggacta
     1201 ataaaagtgt tataatatac ttgaaatgat agtccaaact taaattaaaa aaattatata
     1261 tgatatagaa aaattatatt gaggttaata ctatgtcaaa agaaaaaata aaagtatacc
     1321 tctatacacg agtatctacg tcaatacaga tagaggggta ttcgttaggg gcacaaaaat
     1381 cacgaatgaa agcttttgct atttacaacg attatgaaat tgttggagag tatgaagatg
     1441 ccgggaagtc tggcaagtct attgaaggta gaatacagtt caatcgaatg atggaagata
     1501 taaaatctgg aaaagatgga gtatcttttg ttcttgtgtt taagttatca agatttgcaa
     1561 gaaatgccgc tgatgttctt tctacacttc agataatgca ggattatgga gttaatctta
     1621 tctgcgttga agatggcatt gattcattta aagatgctgg gaaactaatg atttctgttt
     1681 tatcagctgt ggctgaaata gaaagagaaa acatccgtat tcaaacaatg gaaggttgca
     1741 ttcaaaaagc aagggaagga aaatggaatg gtggctttgc tccgtatgga tataaacttg
     1801 aagacggcaa gctgtttata aatgaggaag aggcagttgc aataagaacg attttcgacc
     1861 agtatgtaaa tactacgata ggagccaatg ggatatctaa atacttagag aatcatggaa
     1921 ttagaaaaat cccaagacag aatggtaaga atcctttgtt tgatgcaggt cttataagaa
     1981 agatattaaa gaatcctgta tataatggga aaatagcatt tggaagaaga actttagaaa
     2041 aagttcatgg tacaagaaat gaatataagc aggttgaaca agatgaatat ctaatatctg
     2101 aagggataca tgaagctata gtttccgatg aagtttggca agctgctcag gttaagctaa
     2161 aatctcaagc aaagaaatat gagcgtgtga ataaaggaaa agatacacgc acacacttgc
     2221 tttctggaat tgtaaaatgc ccgatatgtg gagtgggaat gtttgggaac aagtgtatca
     2281 agaaaaagaa agatggcaca aagtataaag atttttatta ctatggttgt aaacataggc
     2341 agatgataag aggtcatgag tgtacattca gtaagcaaat tagagaagaa ttgttagatg
     2401 atgctgttgc agaggtgatt gtcaagatag taagtaatcc gaaatttgct tctatgatgc
     2461 aagaaaaaat caacatgaag gtggatacct ctgaaataga aaaagaaata gataattacc
     2521 aaaaggaatt gagaaagagt cattctacaa agtttaagct aattgaggaa atagataatt
     2581 tagatgttga agataagcat tataagagaa ggaaacagga tttagatgat agactatgtc
     2641 gtatgtatga caaaatagat gaattagaat catcactaat tgatgcgaaa gcaaagaaac
     2701 agactattga agctgaaaag cttacaggag ataacatata taaggttctg atctattttg
     2761 ataagctcta taaagtcatg aatgatgtag agcataggca gttaatttca gctttgattt
     2821 ctgaaattca agtttatgaa gaaaaacaat ctaacggaca atggctaaaa tccattactt
     2881 ttaagctacc aattatagaa gaaaatctca acataggttt ggataatgat gaacaagttg
     2941 aatgtgtaag tttattagag aaacgtagtt aatcctcaaa aggttcccca aaccttttga
     3001 gtcccactga cgcatcacgc tgaggcggta tcactgcttg tacgagttga agcatcagcg
     3061 aagtaaaagt agatgttcag ccaatgcgac gaggtgagac cgaaggtcga taaggtgtgt
     3121 tctgggtata ggactcgtaa agtaagtagg aaatatgatg agatgataca gagagcctga
     3181 attggtcagg tgacgtgctt atttacatta gcctgataat gaaaatggag agattatcga
     3241 tggttatatt ggaattgtac caaaatgatt attctaaaga tttagtagca tttgattcca
     3301 tagaagatgg gaaagccttt gtagctcaaa tcccgggata cactctggaa acagaagatg
     3361 gttttgaagt ggagtatttt aatcctaaaa acataccgga ttatatggaa attattttta
     3421 acggaaatat tgttccttta tctaaattta tgttcgatcc tgaggaaaat gtaaacatta
     3481 tttggaaaga gatttcaaat ttatccctca aaaatgatag agtgatcgaa ggatattcaa
     3541 aaattgatgc atatgtcgtt aacaaccatg aagttaaagc ctatgtcgaa acaagagaaa
     3601 cgaactatcg taaagcaaaa gacttcttag aaagccgcgg atatgaaatc gacagaagct
     3661 ttttcggaag tgaagacggt gaagcgattc tttacagaaa aaaagggatc gaagtttggc
     3721 atttcttgtg tcacttagac ccaatgtttg tagagattga agatgtagaa ggatatgtta
     3781 aagaagaagt tggagagatt caatagtcaa ccaaagttcc ttgtgagtat atacctttgc
     3841 tagtaaaaga gtcaaaacgt tataacgaag ggggggaaga aaacccttga gtgtaagtct
     3901 ttttcttgtt tagagggaaa gtattagagt agccgctcat aatttatact agatttgata
     3961 actgatttga gacagatttt atttctataa atattgtgaa aattttttaa taccaaagat
     4021 ttaaagttac tgtgttaaaa atcctctcca cttttaaact aatgtattat aaaagccagt
     4081 tgacacaagt gttacacctg tgatagaatt aacttgaggt gatgaaaatg gcaaccgata
     4141 aaaatagaat aatgattagc ttggatgata aaaaactaga gaaacttgaa aatttagtag
     4201 aagatgctag agatagaagg ggaatgcgac taacaaagtc ccaggttatt gaattactcc
     4261 taaataccgt tgattatttc gatgatatta tgggagctat ttactcaaaa aaataagagc
     4321 aaaattgcta ttatttttta caaatcggtg taacactagt gatataggag tgatgaaatg
     4381 gttgtttata ttagacaaag taaattacca agtgaggttt caattaataa atacaatgct
     4441 caagtaggtg cctacttaca aggagaagaa gttattttat atcaatcttt ttctgaaata
     4501 aaagagttaa caagtgaaga tatagttgta gattatataa tggagactag agcattacta
     4561 aaaatgatgg gcttaaacgt tccggtttat gactatccta ttgagcttaa agagttttat
     4621 ggtcgaaaga tttatgctgg tattttagga gagattgtga atatacctga taattgggga
     4681 aaatttatta agcctaaagc tggttcaaaa gtctttactg gaagagttgt taatggaacc
     4741 catgatttaa taggtattgg tctacctttc gactatccta tatggattag tgaggttgta
     4801 gaattcatag ctgaatggcg ttgttttgtg ttagatggtc gcgtattaga tgttcgaccc
     4861 tatacaggtg attatcatgc acaatttgat gcaagtgtaa ttgatgaagc gatatcatgt
     4921 tggaaagatg cgccaatagc ttatggacta gatatcggtg ttactcgcga tggcagaaca
     4981 cttgttgttg aagtaaatga tggttatgca ttgggaaatt atggcctatc tcctttaaaa
     5041 tcaatcaatt ttcatagagc taggtggaaa gaaatggtaa aaccctattt tgaaaaaaat
     5101 gaaattttta aaattcaaca ggatgttatt ttctaaaata aatatggtag ataaccaata
     5161 taaaaagcat caaaatgaac aaggaggaat ctgatgcttg gagtaattat tggagatatt
     5221 gtcggttctg tttacgaatg gaacaatatc aaaacaaagg actttccttt atttcggaag
     5281 gactgctttt ttacagatga cacggttatg acctgtgctg ttgcagaagc aattatgaat
     5341 ggtggacaaa aagacgactt cattgacgcg atgaagaaat atggcagaat gtatccgaat
     5401 gctgattacg gtgctcggtt taatgcatgg ctaaacagcg ataaccgtga gccttataat
     5461 agctttggga atggatcagc tatgcgtatt tctccatgtg cttggatcat ggactgtggt
     5521 ttttatgcga aaactggtat atggccatca tctagaggac ttacgagtct ttctgcagag
     5581 gtaactcata atcatccaga aggtgtcaaa ggcgctatgg ctacagctga tgctatcttt
     5641 ctatgtcgtt tttaatttgg tggttattgt agagagtacg agtaatcaat taacgacaat
     5701 cctatagagt gtaaaagacg aattaaggat tatatagaaa aagaatacga ttacaatcta
     5761 tctcaaactt tagatgaaat ccgtcctaac tatcgtttta acgaaacatg tcaggaaact
     5821 gtccctcaag ccattatcgc ctttcttgaa agtagagact tcgaagatgc gataagaaat
     5881 gtcatctcac ttggtggcga cagtgatact ctcgcagcaa tcacttgtag catagcagag
     5941 gcagcttacg gtattcctga ttggatcaag gataaggcct attcttactt ggatgaaccc
     6001 ttaaaggatg tagttaggcg atgggaaaat agaataaaag tgtattaatc tccagtacgt
     6061 cagatttctc ttttctgtat tttcattgta taataggatt acatttactt gcatacgacg
     6121 aaatgtaaac atttctgttt aaaattaagg ataaaatcaa tgaaaaaagc aatggtaatt
     6181 atcaacccta cttctggtgg cgagaaggct ttggattaca aagaaaagct ggagaataaa
     6241 gcaaaagaat actttgaata tgttgaaacc aaaattaccg aaaaagcgct ggatgcaaca
     6301 cattttgctg aagaagcttc tcgtgagcag tatgatgcag tggttgtgtt tggtggagat
     6361 ggaactgtca atgaagtcat ttcaggtatt gatgagagag actacattcc taagttaggg
     6421 attatcccag gcggtacagg taacctcatt acaaaacttt tggaaatcaa tcaagacatc
     6481 gatggcgcaa ttgacgaact ggattttgat ttaaccaata agattgatat cggtaaagca
     6541 aatgacaact attttggtta tatctttagt atcggttctc tgcctgaggc gattcacaat
     6601 gttgaaatag aggacaaaac aaaattcggt attttaacct atgctgtaaa taccatgaag
     6661 tctgtcatga cagatcaggt ctttaacatt aaggttgaga cagaaaatgg aaattatgtt
     6721 ggtgaagcta gccatgtttt ggtccttttg acaaattact tcgctgataa gaaaatcttt
     6781 gaagaaaaca aggacggcta tgccaacatt ttgattctga aagatgcctc tatattctcc
     6841 aaattatccg tcattcctga tttattaaaa ggggatgttg tcgcaaatga taatatcgag
     6901 tatatcaaag cgcgtaatat taaaatctct tcagatagtg aattggagtc agatgttgac
     6961 ggagataaat cagataacct acctgtagaa atcaaagtcc tagctcagcg agtagaagta
     7021 ttttcaaaac cgaaagagga ttagtatata gagaaagcct tttttaaggc tttttgtata
     7081 ctttaaaaga tagttccttt aacaacggac attccttgca aatagtttac aaaaatagta
     7141 tactggattt attgagtttg aaaacgtttg cgtaaaattt gaatgaatac tttaggagac
     7201 aaattgatgg aattgagtgc tatttaccat aggcctgagt cggagtatgc ctatctttat
     7261 aaggataaga aactccatat tcgaattcga actaagaaag gggacattga aagcatcaac
     7321 ttgcactatg gggacccttt tatctttatg gaggagtttt atcaggatac aaaagaaatg
     7381 gtcaagataa cttctggtac cttatttgac cattggcagg ttgaagtgtc agttgacttt
     7441 gcacgtatcc agtatctctt tgagctcaga gatacagaag gtcaaaatat tttgtatggc
     7501 gataaagggt gtgtggaaaa ttctctagaa aatcttcatg caattgggaa tggatttaag
     7561 ttgccttatc ttcatgagat tgatgcctgc aaggttcctg actgggtttc aaatacggta
     7621 tggtatcaga tatttcctga aagatttgcc aatggcaatg ctctattaaa cccagaaggg
     7681 actttagact gggattcatc tgtcacacct aagagcgatg atttctttgg tggtgattta
     7741 caggggatta ttgatcatat ggattacttg caagacttgg gtattactgg actatatctt
     7801 tgtcccatct ttgaatctac aagcaatcac aagtacaata cgacagatta ctttgaaatt
     7861 gaccgtcatt ttggagacag tgttgcatgg gtgcggcaag ggatttttta gaactaatta
     7921 atcacaaagg gctacctctt tttaatccgc ttgtaggtga ttctcatgta aataatagac
     7981 aagaatatga caatacaggg ggtggaaatt tacaacctga aaagtggaat gaaactgcaa
     8041 agcagcttta taatattata atgtggttga ttattttatg gaatgctaag ccggatacac
     8101 ctttatttaa ttttaaagac gaagtaatta agtataaaac atatgagcct tttgaaagca
     8161 gtataaaaag agtaaatact actataaaga atggtagtaa agggaaaact ctgactgaga
     8221 tgattaatgg ctacagagcg gataacgata ttagagatga aatttgtaac tttaatattc
     8281 tgaaaaataa aattcgtgat atgaaagacc aacaaggaaa tacaatggaa tcttactttt
     8341 agttattgtt gaattttggg tattctataa aatatcctaa ttgagattta aatagtagac
     8401 tatacaatat agttaaaata tcagtaaaaa cgacacttta ttgaggtatt ggatacgctt
     8461 tgctaatagc ctaataatca catgtggagt gttgctacaa cgaaaaaggt gataatcctt
     8521 gatttcaagc tattttataa gcattttgtc tttgtagata aaggcaattt tgacaataaa
     8581 aatcctaaaa ggtgaatcgt tatagatgta tttgtagata tcgtttgcgc atcgaaaaaa
     8641 ttaatacaag aataaatatt tatggctctt taggtgactt ttatagaagt aaagtttagg
     8701 atagaaaaac aagaaataac gcaccatttt tggtgcgtta tgctttttta tgctataatg
     8761 gatttataaa aataaaggag tttgctatga ttggaaagaa cataaaatcc ttacgtaaaa
     8821 cacatgactt aacacaacct gaatttgcac gaattatagg aatttctcga aatagcttga
     8881 gtcgttatga aaatggaact agttcagtct ctacggaatt aattgacatc atttgtcaga
     8941 agtttaatgt atcttatgtc gatattgtag gagaagataa aatgctcaat cctgttgaag
     9001 attatgaatt gactttgaaa attgaaattg tgaaagaaag aggtgctaat ctattatctc
     9061 gactctatcg ttatcaagat agtcagggaa ttagcattga tgatgaatct aatccttgga
     9121 ttttaatgag tgatgatcta tctgacttga ttcagacaaa tatctattta gtagaaaatt
     9181 ttgatgaaat agagagatat agcggctatt tggatggaat tgaacgtatg ttagagatat
     9241 ctgaaaagcg gatggtagct taatggaaat ccaagattat actgatagtg aattcaaaca
     9301 tgctttagca aggaatcttc gttcactgac aagaggaaaa aagtccagta aacaacctat
     9361 agcgattttg cttggaggtc aaagtggtgc cggtaagact acaattcatc gtattaaaca
     9421 gaaagaattt caaggaaata ttgttatcat agatggtgat agttttcgtt ctcagcatcc
     9481 acactattta gaactgcagc aagaatatgg caaagacagt gtagaatata ccaaagattt
     9541 tgcaggaaaa atggtagagt ctttagtaac aaaattgagt agtttgagat acaatctttt
     9601 gatagaggga actttacgaa cagttgatgt tccaaagaaa acagcacaac tcttgaaaaa
     9661 taagggatat gaagtacaat tggccttaat tgcgacaaag cctgaattgt cgtatctaag
     9721 tactcttatc cgttatgaag aactgtacat tatcaatcca aatcaagcac gcgcaactcc
     9781 aaaagaacat catgatttca ttgtaaatca tctagttgat aatacacgac aattggaaga
     9841 actggctatc tttgaaagaa ttcaaattta ccaacgagat agaagttgtg tatatgattc
     9901 aaaagaaaat acaacttcag cagcagatgt tcttcaagag ttactgtttg gggagtggag
     9961 tcaggtagag aaggagatgt tgcaggtggg ggaaaagaga cttaatgaat tacttgaaaa
    10021 ataaacaatt gatattttta ggagagtaga aatgagaggg tttaataaca agataaagtc
    10081 tgtttatcaa gaactaacaa attccaaaga gaaattcggt agctttcaca agactttaat
    10141 tcatttgcat acacctgttt cttatgatta caagctattt tctaattgga ctgcaacgaa
    10201 atatagaaaa attac
//
LOCUS       DAVKWZ010000063        10033 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000063 DAVKWZ010000000
VERSION     DAVKWZ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10033
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.063"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(8..166)
                     /locus_tag="VW854_001772"
     CDS             complement(8..166)
                     /locus_tag="VW854_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HEV0262972.1"
                     /translation="MSLLETAKRHQLDSEKYLFYLLECLPNEETLVNKEVLEAYLPWT
                     KVVQEKCK"
     gene            complement(237..683)
                     /locus_tag="VW854_001773"
                     /pseudo
     CDS             complement(237..683)
                     /locus_tag="VW854_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295150.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(759..1193)
                     /locus_tag="VW854_001774"
     CDS             complement(759..1193)
                     /locus_tag="VW854_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0262973.1"
                     /translation="MLLVLVSLAHSFGSVSIIAHIIHQKINLKVPNYRQEEDWARMDL
                     PITRKEISNWHIKASQYYLESLYNLLREKLLEQPLLHADETSYRVLESDSHLTYYWTF
                     LSGKAENQAITLYHHDQRRSGLVVQEFLGDYSGYVHCDMLRQ"
     gene            complement(1269..2120)
                     /gene="rfbD"
                     /locus_tag="VW854_001775"
     CDS             complement(1269..2120)
                     /gene="rfbD"
                     /locus_tag="VW854_001775"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEV0262974.1"
                     /translation="MILITGANGQLGTELRYLLDERNEEYVAVDVAEMDITNEEMVEK
                     VFEEVKPTLVYHCAAYTAVDAAEDEGKELDFAINVTGTKNVAKASEKHGATLVYISTD
                     YVFDGKKPVGQEWEVDDRPDPQTEYGRTKRMGEELVEKHVSNFYIIRTAWVFGNYGKN
                     FVFTMQNLAKTHKTLTVVNDQYGRPTWTRTLAEFMTYLAENRKEFGYYHLSNDATEDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALQE
                     FYKQEVR"
     gene            complement(2186..3235)
                     /gene="rfbB"
                     /locus_tag="VW854_001776"
     CDS             complement(2186..3235)
                     /gene="rfbB"
                     /locus_tag="VW854_001776"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEV0262975.1"
                     /translation="MTEYKNIIVTGGAGFIGSNFVHYVYENFPGVHVTVLDKLTYAGN
                     RANIEEILGNRVELVVGDIADAELVDKLAAQADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQATD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKATIKWYTDNQEWWKAEKE
                     AVEANYAKTQEIITV"
     gene            complement(3232..3840)
                     /locus_tag="VW854_001777"
     CDS             complement(3232..3840)
                     /locus_tag="VW854_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEV0262976.1"
                     /translation="MTDNFFGKTLAARKVEAIPGMLEFDIPVHGDNRGWFKENFQKEK
                     MLPLGFPESFFAEGKLQNNVSFSRKNVLRGLHAEPWDKYISVADGGKVLGSWVDLREG
                     ETFGNTYQTVIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPSLGIEWENIAEAEVSEADKNHPLLKDVKPLKKKICKKERI"
     gene            complement(3841..4710)
                     /gene="rfbA"
                     /locus_tag="VW854_001778"
     CDS             complement(3841..4710)
                     /gene="rfbA"
                     /locus_tag="VW854_001778"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEV0262977.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIRDILIISTPQDLPRFKELLQDGSEFGIKLSYAEQPSPDGLAQAFIIGEEFIGDDSV
                     ALILGDNIYHGPGLSTMLQKAAKKEKGATVFGYHVKDPERFGVVEFDENMNAISIEEK
                     PEYPRSNYAVTGLYFYDNDVVEIAKSIKPSPRGELEITDVNKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEASQYIETVQRMQNVQVANLEEIAYRMGYISREDVLALAQPLKKN
                     EYGQYLLRLIGEA"
     gene            complement(4870..5703)
                     /locus_tag="VW854_001779"
     CDS             complement(4870..5703)
                     /locus_tag="VW854_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEV0262978.1"
                     /translation="MKLTNRVDYFGADISELQNKKLFLFDMDGTIYEEDRLFEGTLEL
                     LDYIHNIGGEYIFITNNSSKSVVDYVEKVNRLGIKAERDNFFTSAQATIVYIKENYPK
                     SKVYCQGTKSLIKELSDAGIDVTEQVSADIDVVLVGFDTELTSDKIRNTCEILSTKDV
                     PFIATNPDIRCPVSFGFIPDCGSICDMISKSVDRKPVYIGKPEPTMVDIVRKKLNYSL
                     FETVVIGDRLYTDIMTGINAGVTSVCVLTGEATVNDIQQDSIKPTYTFKNVKEMWKGI
                     V"
     gene            complement(5718..6422)
                     /locus_tag="VW854_001780"
     CDS             complement(5718..6422)
                     /locus_tag="VW854_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HEV0262979.1"
                     /translation="MKALILAAGLGTRLAPITNEVPKSLVPVNGKPILMKQIENLYQN
                     NITDITIIAGYKSSVLTDAVTEKYPEINIIDNVDFKTTNNMYSAYLGKAAMGDSDFLM
                     MNADVFYDASVIKSLLLHKAPNAIVTDLGIYIEESMKVVEKNGRLVEISKQISPEETL
                     GASIDVYKFSYEAGARFFEKCKEFIEDKRELQMWSEVALNAILSEVEFVACPLEGRWL
                     EIDNHEDLVAAEKLFA"
     gene            complement(6438..7466)
                     /locus_tag="VW854_001781"
     CDS             complement(6438..7466)
                     /locus_tag="VW854_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEV0262980.1"
                     /translation="MKNNDLKIGSGAIHQISATLSQNSISGKILYCADPVVDDLYGSI
                     VRSQIEEIGRVKEESCNYNTIAYAMNIAERAIATDIDCIVGMGGGRVLDVCKYASFIS
                     KRPYLSIPTTAANDGIASPVAVLKRQDDRPKSLGAAIPSMTLIDIDVIASGPIQNIKA
                     GIGDTISNYTALKDWELAVERGKDEMHGFAYLMSQNSLDALMKTKYNSITPDFIEVLV
                     NSLVLSGIAMDFAGSSRPVSGSEHLFSHALDYYGSTRNLHGIQVALGTVAVLKLIENS
                     VDTVVDYLQRFEVHINPKLLGIDEELFIYCMQHATKMRSNRYTYLHEVDLSTDRLKQI
                     YKELISEL"
     gene            complement(7502..8650)
                     /locus_tag="VW854_001782"
     CDS             complement(7502..8650)
                     /locus_tag="VW854_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HEV0262981.1"
                     /translation="MNILQYIKILARTIFMLLISTVLLPVRLKNNKILFINFNGKGYG
                     DNPKSICEYLRTTYPDLDLVWLARDNEGFPDGVRVVKYGTFQAFYEQASSKVWVYNVR
                     AFARILKKRGQIYIQTWHGASSFKLIEKQADLPINYVLEAKYDARVTDIMISDSRKQT
                     EEFQKYFWYSGEIFEVGMPRNDALFHYKEDYDKLNNIRKELSIHSDDYVILYAPTFRD
                     DGDASYLDINFERLLQCVEHGIKKKCKFLIRLHPNHSHLCNNISFNKNIINATFYSDM
                     QELTLLADVLVTDYSSSIFDFMLLNKPYVRYVNDLEKYAELRGVSDTYYELPDSIIKT
                     AEELYDLLPKKIENFDYDSIKKYRNEILCPIFNGTASENVGRRIIQEL"
     gene            complement(8653..>10033)
                     /locus_tag="VW854_001783"
     CDS             complement(8653..>10033)
                     /locus_tag="VW854_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEV0262982.1"
                     /translation="YKELAKNTGTFALANFSSKILIFLLVPIYTKVLTTTEYGFYDLV
                     YTTIQLLVPILTLNISEAVMRFLMKEDVSKKSVFSIAILDIFLGSIIFCLLLLVNQIF
                     SLSELISQYSIYIMAIFAFYTLNNFLIQYSKGIDKIGVTAISGVISAAVMLSMNILLL
                     VVLNWGLLGFFIANICGYVIPCVYIIVKLKLWDLFELKIDRSLQWEMIYYTLPLILNT
                     LSWWVNNTSDRYIITVIIGIQASAIISVAYKIPQIFSTISAIFIQSWQISAIKIQEEK
                     EGNTFISKMLLYYNALLLIIASGIILFVKPISNILFGASFYSAWTLVPFLIISSLFNA
                     ISGYIGAIMGAKMDTKNIAKSALVGMIANVFLNIVLTFLMGLQGITISTMIASFLIFY
                     MRKDSVEEIAPETYRAIYLSWFLLVVEASLLVYIDFIIGATLVTLINLFLLKDTLKPL
                     CLKLLKGFK"
BASE COUNT         3176 a         2098 c         1525 g         3234 t
ORIGIN      
        1 agatttctta tttgcacttt tcttgtacaa ctttagtcca tggtaaataa gcctctaaaa
       61 cctctttgtt tacgagagtt tcctcgtttg gaagacattc tagaagatag aatagatatt
      121 tctcgctatc taattgatga cgtttagctg tttccaacaa actcataata atagctgttg
      181 ctttagctcc ttcaaaactt tgagaaaata accaccctcg ctttttaaaa tttagcttag
      241 gctaaaagag tccactgaac tcttttactc cgtcccataa ccaatgattt aatggcgcgt
      301 tcagctagat tattggaaag gaccagatgt ccgtctttca aaatagtctt aaaggtttct
      361 tcatacttga ggctgtattc aattgccctt cctagttttg aacctgctaa aactgactga
      421 cgccggcact aagcaaagaa gtcttccatt aggggttgga gctcttcttg acgtttctgt
      481 agtcgttcat cagctggcaa agcctcccag tctctttcca gggaaaataa ctgatcacaa
      541 taagctaaac ctttagcttc taaggatgat ttatctgctt gcttgggggt cgcttcaaaa
      601 aacttccttc tcacatgtgc ccaacatcca acaagttcag cttcttccag ttgacgataa
      661 gcttctagcg gttcgcagtt taccaagctt agcgtcgcct tgctcctaaa ccttggactg
      721 ctatcgcata ggcagaacta gaggactaaa gtcctaagtt actgccgcaa catatcacaa
      781 tgcacatagc cagaataatc tcctaggaat tcttgtacta ctaaaccact ccgacgctga
      841 tcatgatggt acagcgtgat tgcttgattc tcagctttcc cagacaaaaa ggtccaatag
      901 taggtcagat ggctatcact ctctagaacc cgataagagg tttcatccgc atgaagaaga
      961 ggttgttcta acaacttttc tcgtaaaagg ttataaaggg actctaaata gtattgactt
     1021 gccttgatat gccaattaga gatttcctta cgtgtgattg gtaaatccat cctagcccaa
     1081 tcttcttctt ggcgataatt gggtaccttc agattaatct tctgatggat gatgtgagcg
     1141 ataatagaaa ctgagccaaa gctatgcgcc aaagagacca gcaccaacaa caagataatc
     1201 gtacatctta agctcctttg gagttcatta tctgcctctt ttattagact agaaaatcat
     1261 tctactactt atctcacttc ttgtttgtaa aattcttgca atgcatcttg ccaagttgga
     1321 ataacaaatc cagtagcttt ggctttggcc aggctcatcg ttgagtttag cggacgttta
     1381 gctttggctg gaaattgact ggaatctact ggcttgactt cgacatctgt atctttcaaa
     1441 atttcaactg caaaatcata ccatgttgtg tcttctgtcg catcatttga caaatgataa
     1501 taaccaaatt ccttacgatt ttcagctagg taggtcatga actcagccaa ggtacgagtc
     1561 caagtcggac gaccgtactg gtcatttaca actgttaaag tcttatgagt tttcgcaaga
     1621 ttttgcatgg taaaaacgaa gtttttgcca taatttccaa atacccaggc agtacggata
     1681 atatagaaat tagacacatg cttctcaact aactcttccc ccatacgctt agtgcgtcca
     1741 tattctgtct gtggatctgg tcggtcatca acttcccact cttgtccaac tggtttctta
     1801 ccgtcaaaga catagtccgt agaaatataa actagagttg caccatgctt ttcagatgct
     1861 tttgcgacat tttttgtccc cgtcacattg atggcgaagt ccaactcttt tccttcatcc
     1921 tctgctgcat caacagcggt gtaggctgca cagtggtaga ctaaagtcgg tttcacctct
     1981 tcaaaaactt tttcaaccat ttcttcattg gtaatgtcca tctcagccac atctactgcc
     2041 acgtattctt cattacgttc atccaataaa tagcgaagtt ccgttcctaa ttggccattt
     2101 gcccctgtaa ttaaaatcat ctctagacct ttctaatatg taactcttcc caatatagca
     2161 ataaaaagca gctatttcct gctttttata ctgtaataat ctcctgagtc ttagcataat
     2221 tggcttcaac agcttctttt tctgctttcc accattcttg gttatctgta taccacttga
     2281 ttgttgcctt gagcccagct tcaaagttgg taaattcagg tttccacccc aactcatcac
     2341 ggagcttgct ggcatcaatc gcatagcgaa ggtcatgtcc tgcacggtca gtcacatgat
     2401 cataggcatc cgtagcttgt cccatttcct taaggataag ttccaaaact tccttattgt
     2461 tcttctcacc atcagcccca atcaagtagg tttcaccgat ttgccctttt gtcaagattg
     2521 tccaaactcc tgaagaatgg tcattggtat gaatccagtc acgaacattc ttaccttcac
     2581 cgtaaagttt tggcttaatt ccacttagga tgttagtaat ctgacgtggg atgaattttt
     2641 cgatatgttg ataaggaccg tagttatttg aacagttgga aatcgttgcc ttgactccaa
     2701 aagaacgcac ccaggctttg acaatcaaat ctgaggctgc cttggttgat gagtacggag
     2761 agcttgggtt gtattttgtt tcagcagtaa atttctcacc tggtccttca ccatgacctg
     2821 gcaaatcttc gcgtaaaggg agatccccat aaacttcatc tgtcgataca tggtggaagc
     2881 gaatatcata cttacgagca gcttctaaaa gagtataggt tccgatgaag ttagtatgaa
     2941 taaatggcga tggatcattg agcgaattat cattgtggct ttccgctgca taatgaacga
     3001 tagcatctgc ttgagcagcc aacttgtcta ccaactccgc atcagcaatg tcaccaacaa
     3061 ctaactcaac acgattacct aaaatttcct caatattcgc gcgatttcca gcataagtca
     3121 acttatctag gactgtcacg tgaacacctg gaaagttctc gtaaacataa tggacaaagt
     3181 tagaaccgat aaagccagct ccacctgtca cgataatatt tttgtattca gtcatattct
     3241 ttccttttta caaatcttct ttttcaaagg ttttacatcc ttaagtagtg gatgattttt
     3301 atctgcttct gaaacttctg cttctgcaat attttcccat tcaataccaa ggcttggatc
     3361 agcgtagttc acaaaggcat acttgggttt gagttcaaga gcccagtaat cattgaccag
     3421 atagctatat gacactgtat ctgataaaac ttggaagcca ttagctacgc ctcgaggaac
     3481 aaagattccc ttactcgcat caatcactgt ctgataggta ttcccaaagg tttcaccctc
     3541 gcgtagatca acccaagaac ccagaacttt ccctccatct gctacagaga tgtacttatc
     3601 ccaaggctct gcgtggaggc ctcgaaggac atttttacgt gagaaggata cattgttttg
     3661 caattttcct tctgcaaaga aagactctgg aaatccaagt ggaagcattt tttccttttg
     3721 gaaattttct ttaaaccagc cacgattatc tccatgaacg gggatatcaa actccaacat
     3781 gcctggaata gcttcaacct tgcgtgccgc aagcgtctta ccgaaaaaat tatctgtcat
     3841 ctatgcttct ccaatcaaac ggagtagata ctgtccgtat tcatttttct taagtggttg
     3901 ggctaaggcc aacacatctt cgcgactgat atagcccata cggtaagcaa tttcttccaa
     3961 gtttgctacc tgaacatttt gcatccgttg gactgtttcg atgtactgcg aagcctctag
     4021 taaactttca tgagtgccag tatccaacca agcaaaacca cgtcccatga gctcaacaga
     4081 caaatcacca cgatccaagt aagccttgtt gacatctgta atttccaatt caccacgagg
     4141 acttggttta atacttttgg caatctctac tacatcatta tcatagaaat agagtcctgt
     4201 cactgcatag tttgaacgag ggtattctgg cttttcttcg atggagatag cattcatatt
     4261 ttcatcaaat tctacaacac caaagcgttc tggatccttg acatggtagc caaaaacagt
     4321 cgcacctttc tctttcttgg ctgctttttg aagcattgtg ctcagaccag gaccatggta
     4381 gatattgtca cctaaaatca aggcaacgct atcgtcaccg ataaactctt ccccaatgat
     4441 aaaggcttgt gccaaaccat ctggacttgg ttgctcagca taagaaagtt taataccaaa
     4501 ctcagaacca tcttgaagaa gctctttaaa acgaggcaaa tcttgaggag ttgagataat
     4561 caaaatatcc ctaatcccag ccaacattaa tgttgaaagt gggtagtaaa tcatcggttt
     4621 atcataaacc ggcataagtt gttttgatgc agcgcgagtc aaaggataca agcgagttcc
     4681 cgaaccacct gctagaataa tacctttcat aaaaggggta cctttctttg ttgatgagta
     4741 ctttacgttc acgaacgatt tggggagaaa aataatttta atttgtccat tattaaaata
     4801 ataaaatcaa tttaaataca tacgagtatt ttgacagtat tcatgaattc actattcaga
     4861 atgaaaaaat cagacaattc ctttccacat ttctttcacg tttttaaatg tataagtcgg
     4921 ttttatacta tcttgttgaa tatcattcac cgttgcttct cctgtcagca cacaaactga
     4981 agttactcct gcatttatac cagtcatgat atccgtatac aagcgatctc caatcacaac
     5041 tgtttcaaat agagaataat ttaatttttt tcgaacaata tcaaccatcg taggttcagg
     5101 tttacctatg taaacaggtt tcctatcgac tgatttacta atcatatcac aaatagaacc
     5161 acaatccggg atgaatccaa acgatactgg gcagcgaata tcagggttag tagctatgaa
     5221 aggtacgtcc tttgttgata gaatctcgca ggtattgcga attttatcac tggttaattc
     5281 tgtatcaaaa ccaacaagaa caacatctat atcagcacta acttgctcag ttacatcaat
     5341 tcctgcgtca gatagttctt ttatcaaaga ttttgttcct tggcaataaa ctttagattt
     5401 aggataattt tctttaatat aaacaattgt ggcttgagca gaggtaaaaa aattatctcg
     5461 ttcagcttta atacctaatc tgttaacttt ttcaacatag tcaacaacag acttagatga
     5521 attgtttgta ataaaaatat actcaccgcc aatattatga atatagtcta ataattcgag
     5581 agtaccctca aacaacctat cttcttcata aatggtacca tccatatcaa ataagaataa
     5641 ttttttattc tgaagttcac taatatcagc accaaaataa tcaactctat ttgtcaattt
     5701 cattctccgt attcggttca agcaaatagt ttttctgcag caactaagtc ttcatgatta
     5761 tcaatttcta accaacgacc ctctaatgga catgctacaa attcaacttc agaaagaatt
     5821 gcattaagag caacctcact ccacatttga agttctcgtt tatcttcaat aaattcctta
     5881 cacttttcaa agaatcgagc acctgcttca taagagaatt tataaacatc aatagaagct
     5941 cctaaagttt cctcaggtga aatctgttta gaaatttcta ctaaacgacc atttttttct
     6001 acgactttca tagactcttc aatataaata cctaaatcag ttacaattgc atttggagct
     6061 ttatgaagca acagactttt aataacagaa gcatcataaa atacatctgc attcatcatt
     6121 aagaagtcac tatcacccat tgcagctttt cctagatagg ctgaatacat attattagtc
     6181 gttttaaaat caacattatc aataatatta atttctggat acttttcagt aactgcatct
     6241 gttaatacag atgacttata tccagcaata atcgtaatat ctgtaatatt attttgatat
     6301 aaattttcaa tttgtttcat caaaattggc ttaccattga ctggtaccaa agattttggt
     6361 acctcattgg taattggagc tagacgtgtt cctaaccctg ctgctaaaat aagtgctttc
     6421 attttttcct ctcttctcta caactcgctt atgagttcct tatatatttg tttcaatcta
     6481 tctgtgctaa gatcaacttc atgcagatag gtatagcgat tacttctcat ttttgtagca
     6541 tgttgcatac aataaataaa caactcttca tctatcccca aaagtttcgg gttaatatga
     6601 acctcaaatc tttgcaagta atccactaca gtatcaacag aattttcaat taatttcaaa
     6661 actgcgactg tacctaatgc aacctgaatt ccatgaagat tccttgtaga accatagtaa
     6721 tctaatgcat gactaaataa gtgctctgac ccactgacag gtctactact tcccgcaaaa
     6781 tccattgcaa tgcctgataa aactaaagag tttaccaaaa cttcaataaa atcaggggta
     6841 atagaattat acttcgtttt cattaaagca tctaaagaat tttgcgacat tagatatgca
     6901 aaaccatgca tctcatcttt ccctcgctca actgccaact cccaatcttt caatgcagtg
     6961 taattggata ttgtatcacc gataccagct tttatatttt gtataggtcc tgatgcaata
     7021 acatcaatat caattagtgt cattgagggg atagccgccc ctaagctttt tggtctatca
     7081 tcctgccttt tcaaaacagc aactggggaa gcaatgccat catttgctgc tgttgtcgga
     7141 atcgataggt aaggacgttt cgaaataaaa gatgcatact tgcagacatc taaaacacga
     7201 cctcctccca ttcccacaat acagtcaata tctgtggcaa tagcccgttc tgctatattc
     7261 atcgcataag caatagtatt ataattgcaa ctttcttcct ttacccgacc aatttcctct
     7321 atttgtgaac gtactatcga accgtacaag tcatcgacaa ctggatcagc acaatataaa
     7381 atctttcccg aaatactatt ttgagaaagt gtagcggaaa tttgatgaat agctccactt
     7441 cctatcttta aatcattatt tttcaaatct cgttacctta aaaataaccc aatgttttac
     7501 ttcacaactc ttgaattatt ctccttccta cattttcact agcggttcca ttaaagattg
     7561 gacacagtat ttcatttcta tattttttta tagaatcata atcaaaattt tctatttttt
     7621 taggcagtaa gtcatacaat tcttcagcag ttttaataat cgaatccggc aattcgtaat
     7681 atgtatctga taccccccta agctcagcat atttttctaa atcattcaca tatctgacat
     7741 aaggcttgtt taaaagcata aaatcaaata tcgaggaaga ataatctgtg actaaaacat
     7801 ctgccaacaa agtaagctct tgcatatctg aataaaacgt agcattgatt atattcttat
     7861 taaatgaaat attattacac aaatgggagt gattagggtg taacctaatt aagaatttac
     7921 atttcttttt aattccgtgt tcaacacatt gcaaaagccg ttcaaaattt atatctaaat
     7981 aagatgcatc cccatcatct ctgaaagtag gggcatataa aataacataa tcatctgaat
     8041 gaatgcttag ttcttttctg atattattta acttgtcgta gtcttcctta tagtgaaaca
     8101 aggcatcatt tctcggcatc cccacctcaa aaatttctcc tgaataccaa aaatactttt
     8161 gaaattcttc agtttgtttt ctgctatctg aaatcattat atctgtgact cttgcatcat
     8221 acttagcttc taaaacataa tttattggta aatcagcttg tttttctatc aacttaaaac
     8281 tagaagcacc atgccaagtt tgaatataaa tctgtcctcg tttttttagt attcttgcaa
     8341 aagctcttac attatatacc caaaccttcg aactcgcttg ctcataaaaa gcttggaaag
     8401 tcccatattt aacaacccta accccatctg gaaaaccttc gttatctctt gcaagccaaa
     8461 ccaaatctaa atctgggtaa gtagttctaa gatactcaca aatactttta ggattatcac
     8521 cataaccctt cccattgaaa ttaataaata gaatcttatt attttttaac ctgacaggta
     8581 ataacactgt acttatcaat agcataaaaa tagttctagc caaaatttta atgtattgta
     8641 gaatattcat tttcatttaa atccttttaa taattttaaa cataatggtt taagagtatc
     8701 ctttaataaa aataaattga ttaatgtaac taatgtagcc ccaataataa aatctatata
     8761 aaccaaaaga ctagcctcaa ccactaacaa aaaccacgaa agataaatag ctctatatgt
     8821 ttctggagca atttcttcaa cactatcttt tctcatataa aaaataagaa aactagctat
     8881 catagttgag attgtaatcc cctgtagtcc cattaaaaat gttaatacaa tatttaaaaa
     8941 aacattagct atcattccaa ccaaagcaga tttagcaata tttttagtat ccattttagc
     9001 gcccattatc gctcctatat aacccgaaat cgcattaaac aaactgctaa taattaaaaa
     9061 cggtacaagc gtccacgctg aataaaatga agctccgaag agtatattag agattggttt
     9121 tacaaataga atgataccgc tcgctataat cagcaagagt gcattataat ataaaagcat
     9181 ttttgatata aaggtgttac cttctttttc ttcctgaatt tttattgcag atatttgcca
     9241 cgattgaatg aatatagcgc taattgtgga aaaaatttgt ggaatcttat aagcaactga
     9301 aataatcgca ctagcttgta tacctataat tactgttata atatacctat ctgaagtgtt
     9361 attaacccac caacttaaag tatttaaaat caaaggtaaa gtataatata tcatttccca
     9421 ctgtaacgat ctatcaattt taagttcaaa taaatcccat aattttagtt ttactattat
     9481 atatacacaa ggaatgacat acccacaaat atttgcgata aaaaaaccta gtagtcccca
     9541 atttaatacc actaacaata aaatattcat cgaaagcata actgctgcac ttatgactcc
     9601 agagatagcc gttacaccta ttttatcaat ccctttagag tattgtatca aaaaattatt
     9661 aagtgtataa aaagcaaaaa tcgccatgat atatatgcta tattgagaaa ttaactccga
     9721 taaagaaaat atttgattga ccagtagtaa caaacaaaaa ataatagacc ctaaaaatat
     9781 atcaagtatt gcaatcgaaa aaactgattt ttttgaaaca tcttctttca ttaaaaatct
     9841 cataactgct tcagatatat tcaatgtcag gattggtaca aggagctgaa ttgttgtata
     9901 aaccaaatca taaaaaccat actctgtcgt tgttagaact tttgtatata taggtactaa
     9961 caaaaaaatt aatatttttg atgaaaaatt agctaaagca aaagtacctg tattttttgc
    10021 taattcctta tat
//
LOCUS       DAVKWZ010000064         9895 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000064 DAVKWZ010000000
VERSION     DAVKWZ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9895
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.064"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            1..383
                     /locus_tag="VW854_001784"
                     /pseudo
     CDS             1..383
                     /locus_tag="VW854_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            386..1399
                     /gene="efeB"
                     /locus_tag="VW854_001785"
                     /pseudo
     CDS             386..1399
                     /gene="efeB"
                     /locus_tag="VW854_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259617.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
     gene            1374..1952
                     /locus_tag="VW854_001786"
     CDS             1374..1952
                     /locus_tag="VW854_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron permease"
                     /protein_id="HEV0262983.1"
                     /translation="MVRNYWKAKSWLLVLALSFLILSPAGAQESLSFFFVKITDASKT
                     VKNGGQTETQKLVTKMASDFERVENKDSEVGKIVKEKLALSGDITEAKLTEISSALLA
                     FEKEQNPVDLDAEKEKLVNRLSPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVV
                     RDNSTAHYGRVETAISFLPSSMETEPTDESGT"
     gene            complement(2009..2293)
                     /locus_tag="VW854_001787"
     CDS             complement(2009..2293)
                     /locus_tag="VW854_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEV0262984.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(2347..3282)
                     /locus_tag="VW854_001788"
     CDS             complement(2347..3282)
                     /locus_tag="VW854_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEV0262985.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQEVLKQYNVTDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            3386..3829
                     /locus_tag="VW854_001789"
     CDS             3386..3829
                     /locus_tag="VW854_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEV0262986.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELAAKVG"
     gene            3945..4211
                     /locus_tag="VW854_001790"
     CDS             3945..4211
                     /locus_tag="VW854_001790"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEV0262987.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVRSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            4208..4582
                     /gene="crcB"
                     /locus_tag="VW854_001791"
     CDS             4208..4582
                     /gene="crcB"
                     /locus_tag="VW854_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV0262988.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIFLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDAVKLLDTGRYLSLI
                     LYLLLSIGGGLLLAYYLGRKKW"
     gene            4576..4905
                     /gene="crcB"
                     /locus_tag="VW854_001792"
     CDS             4576..4905
                     /gene="crcB"
                     /locus_tag="VW854_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV0262989.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            5024..5371
                     /gene="rplS"
                     /locus_tag="VW854_001793"
     CDS             5024..5371
                     /gene="rplS"
                     /locus_tag="VW854_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEV0262990.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVGRIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            5413..5484
                     /locus_tag="VW854_001794"
     tRNA            5413..5484
                     /locus_tag="VW854_001794"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5446..5448,aa:Arg,seq:cct)
     gene            complement(5539..6345)
                     /gene="yidA"
                     /locus_tag="VW854_001795"
     CDS             complement(5539..6345)
                     /gene="yidA"
                     /locus_tag="VW854_001795"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEV0262991.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYERYSINKSAPFYLELFTKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            complement(6358..7662)
                     /locus_tag="VW854_001796"
     CDS             complement(6358..7662)
                     /locus_tag="VW854_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV0262992.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSHRFVNRKVFKSITFSQENQDQLASMRKLAE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            7734..8111
                     /locus_tag="VW854_001797"
     CDS             7734..8111
                     /locus_tag="VW854_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEV0262993.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTNRYQVDGQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            8333..>9895
                     /locus_tag="VW854_001798"
     CDS             8333..>9895
                     /locus_tag="VW854_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000921212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEV0262994.1"
                     /translation="MLKTQEIADKYGITRQTLNNWMQKGIISQPRKNNRSAYEWEEEN
                     EKEIVRVISEEIPAKYYVSNKETLKIGNRRYLGSKQKMLDFILKTVSENIGSIDSVAD
                     IFGGTGVVADLFRKQNKKVIVNDILYSNFISFQTWFSNEDVDIHKVSHIIDELNNLSP
                     KKGYVSKNFGGAYFSEENAGKIDSIREEIEKYKSGNQREYFMLLTSLLYAMDKVANTV
                     GHYDAYRKKMDYCKEIYLRVPEYNENNQNEIYNKDANKLVKEIYADLVYIDTPYNSRG
                     YENAYHVLENIAEWKKPDVEGVAKKAVNRSEKGSDYTKSKAPQAFEDLILNINAKYIL
                     VSYNNMNKKGNSRSNAKISNEEIIKILSKRGKVQVFETDFSPFTTGKSKIENHKELLY
                     LCIISPEKKKEKKLIKSALNYTGGKYKLLPQLLPLFPESYNNFIDLFSGGATVAVNLA
                     NINKSKTKKYIINDISEEVIDFYKYLESQKDVTVFLDKVEKAIEFYKLSNTQKYSYAH
                     YGVNSSTGLGSYN"
BASE COUNT         2959 a         1724 c         2214 g         2998 t
ORIGIN      
        1 aaatgtaaaa aaatatgagg agttcggact cgactctctc cttcaagaaa cacgtggtgg
       61 tcgtaaccat gcatatatga cagttgagga agagaaagcc tttcttgccc gtcatttgaa
      121 ggctacagag gcaggagaat ttgttacaat tgatgcctta tttcaggctt ataaaaagga
      181 gttaggtcgt tcctacacac gtgatgcctt ctatcaactg ttgaagcgcc atggttggcg
      241 aaatattaca ccacgtccag aacatcctag gaaagcagac gctcaaacca ttgtcgcgtc
      301 taaaaataaa atctcaattc aagaagacaa gaaagcgctt taaaaccagt agaccttttc
      361 ataaggttcg cttgatgtac caaatagtac gcccatgcaa aagaatatct attttgttgt
      421 cttggacctg catacgacgg atagagataa aattatccag ctattcaagg attggacgga
      481 ttatagtgcc aagttggtcg agggggaatt ggttaaaaaa gatggacaga atgctctctt
      541 tcctccgagc gatactggtg agaaagttgg gctcaatcct catcgtttga cgctgacttt
      601 tggtgtatct gcaagtttct tgaaaaggat gaacttggaa aacaagcgcc ctcgactgtt
      661 cagggattta cctctatttc ctaaggagca attacgtgaa aaatatactg gtggagatat
      721 tgtgattcat gcttgtgcag atgatgagca aattgccttt cacgctattc gcaatctcat
      781 ccgtaagggg agaaatgcgg tgccccttcg ttggagtcag tctggatttg cagctatcgg
      841 ggatcgaatg gagactccgt ggaacctctt tggttttaag gatggaacag caaatccaac
      901 caaggagcaa gactttgacc gcgttatctg ggctgatagt aaggactgga tggaaaatgg
      961 ttcttatatg gcagttcgtc ggattcagat gtttttagag acctgggacc gcactagtct
     1021 cgaagaacaa gaaaatacct ttggtcgtta caaggaaagc ggtgccccct ttggtaagaa
     1081 aaatgagttt gatgaagtgg atttgagtct cttacctgat gattcgcatg tttgtttggc
     1141 caaagaagtg gataagccac ttttacgtcg ttcttattct tactcagatg gcattgatga
     1201 aaagactggt cagtttgata cgggattgct cttcatttct ttccagaagg atcctgataa
     1261 ctttgttaag gttcaaacta acctaggtgc tactgataag atgaatgaat acatcactca
     1321 cataggtagt ggacttttta cttgctttgg tggtgtggag aaaggaggct acattggtca
     1381 gaaattattg gaaggctaaa agctggttac tagttttagc tttgtcattt ttaattcttt
     1441 ccccagcagg tgcccaagaa agcttgagtt ttttctttgt gaaaatcaca gatgcatcca
     1501 aaacggttaa aaatggtggt cagactgaga cccagaaact cgttacgaag atggcatcag
     1561 acttcgaaag agtagaaaat aaggattctg aggttggtaa gattgttaag gaaaagttag
     1621 ctctatctgg agatattact gaagccaaac tcaccgaaat ttcctcagct ctattagctt
     1681 ttgaaaaaga acaaaatcct gttgacctag atgcggaaaa ggaaaaattg gtaaaccgtc
     1741 taagtcctcg ttttgaaacc ttagaacagg ccattgcctc caaagatttg gaaaaagtcc
     1801 gagaagcttt taagaaaatg aattccactt ggaccatcaa tgaaagtgtg gttcgtgaca
     1861 atagtacagc tcattatgga cgtgttgaaa cagctatttc cttcctgcct agtagcatgg
     1921 aaaccgaacc tacagatgag tctggaacat gaaaacagca gttgatttca agaaacaaaa
     1981 aagaacagcc gaaactgttc tcttatcatt atttgagacc gtattttttg ttgaaacgat
     2041 ccacgcgtcc atctgcctga gtgaactttt gacgtccagt gtagaatggg tgtgagtctg
     2101 atgaaatttc cacacggatc aatgggtaag tttcgccttc gaactcaact gtttcgttag
     2161 agcgttttgt tgaaccgcta aggaattggt aaccagtagt tgtgtccatg aagacaactg
     2221 ggcgatattc tggatggata tcttttttca ttttgcaata tttcctttct gccatggtct
     2281 ctttgcgagc catagattgt taccatacta gtctatcaga ttctgaaaag tttggcaagt
     2341 attttatcag tttttaagca agttttttaa cttttggtag atgatttcgt tttcttctag
     2401 gctataggaa ttagcaccac ttgctagagg gtggcctcca ccatcatgct ccttggcgat
     2461 ttcattgata ggatggattt tactgcgtaa gcgaactcgg tagtggccat cagcctgttc
     2521 gacaaaaatt ccccagagac tcactctgtc aatgcgtcca ggtgcaccga caatggctgc
     2581 agtttcagca tcggttacat tgtattgttt caagacttct tgactcagga taacgcgagc
     2641 agcaccattt tcatccactt ccagatggtc gtagatgtag ccttgcagtt tagcaatttt
     2701 gtagctcata gtgtccattt tgcgagtgag agccgcaaag tcaaagttat gttctctcaa
     2761 ataagcagcc aggcgaagag tccgtgcagt ggtagaaggg tagaggaagc gacctgtatc
     2821 accgacaatt cctgcaaaga gcaactcagc atcgcgatct gccaaggcta gttgggttgt
     2881 ttgggcaaat agggtaatca tctcgctagc gctacttgaa ctagtatcga cccaagacag
     2941 gtcaccgtat acatcatcat ttggatggtg gtcaatctta atgagaaaat caccttgact
     3001 atagcgctta tcatcgatac gagcagtatt agctgtatca cagacgatga caagtgcgcc
     3061 ttggtaggct ctatcttcaa caagatccat ctcagccatc caagtaagag ttggttcatc
     3121 aaaaccgacg gctttgatgg ttttttctgg gaaatgatgt tccagcaagg ctttcaatcc
     3181 cacctgactt cccaaggcat cagggtctgg tttcatatga cgatgaatga taatggtgtc
     3241 gtattcttta attttttcta aaatttgttg gcaaatctcc ataaataacc tcaattcttt
     3301 ctcatttcat tgtagcacaa gaatttttat ttttaaaatg aaaaagatgt gatataatgg
     3361 agaaagataa attgaggagg acgatatggc attagcaaaa attgtatttg ccagtatgac
     3421 cggtaatacc gaagaaattg cagatattgt agcagacaaa ttacgtgact tgggcttgga
     3481 tgttgatgtt gatgaatgta caactgttga cgcttcagac ttcttggaag cagacatcgc
     3541 tatcgttgcg acctatactt atggtgatgg tgaattgcca gatgagatga tggacttcta
     3601 cgaagaccta gcagatctca acttgaatgg caaaatctac ggagtggtcg gctcaggaga
     3661 taccttctac gatgaattct gtaaggctgt tgatgacttt gaccgtgtct ttgtgtcaac
     3721 aggagcagaa aaaggttcag agtgtgttaa agttgatctt tctgccgagg aagaagatat
     3781 tgaacgcttg gaacaattcg cagaagaatt ggctgctaaa gtaggataag cataaaagtg
     3841 cgctgatgta atcaatcagt gcatttttct tatcgagagt tgggatttta ctagcctaat
     3901 tgatggcttt ttgatacaat aattcaaata aaggaggaga ctgtatggat ttagatatta
     3961 ttcgtcaaga aatcgatcaa atcgacgacc aaattgtcaa actgttagaa gaacgaatgc
     4021 atttagttga gggggtggtt gcttataaga aagcttcagg taaaccgatt ttagatacta
     4081 agagagaaga agttattttt gaaaaggtta ggagtcgtgt agaggacaag cgctatcagg
     4141 agactattgt agcgactttt tcggacatac tcaaacgttc gcgtgattat caggatcaaa
     4201 atatcaaatg aaaaaagaac aattttatcc gctaggaatt tttctagctg ctatgttggg
     4261 cggacttgtc cgatatctag tttccacctg gttaccagcc agtccagact ttccttgggg
     4321 cactctcttt gtcaactatt tgggaatttt ctgcttgatt tttcttgtca agggctatct
     4381 ggtctataag gggactagta agggcttgat tttagcactg ggtacaggtt tttgcggagg
     4441 tttaacaacc ttttctagtc tcatgcttga tgctgtgaaa ttactggata cagggcgtta
     4501 tcttagtttg atactgtatt tgcttttgag tatcggtgga ggcctgcttt tagcttacta
     4561 tttggggagg aagaaatggt aatcgtctat cttgcaatcg cctgtggctt gggagcactc
     4621 gtacggtatt tcttttcccg ctacaatcaa gcttctaaat tgccactcgg aacgctcata
     4681 gctaatcttt taggttgttt tttaattgga gtattctaca atcatgtaga gtctaaggaa
     4741 gtatatgcta ttctagcaac aggattttgt ggcggtttaa caactttttc gaccttgaat
     4801 gacgaacttc aaagactgct aagtgataag aaggtctttt attcttacct gactttgact
     4861 tacataggtg gtttggttgc gattttttta ggaattttgc tataaatttc tttacttttt
     4921 tgtgaaaatt tggtagactg tatcttgtgt gtgatggacg cacaaaaata cagtttatcc
     4981 gctgaggaag tttcctcaag attgacaaag ataggagaat aaaatgaatc cattaatcca
     5041 aagcttgact gaaggtcaac ttcgtacaga tatcccatca ttccgtcctg gtgacactgt
     5101 tcgtgtacat gcgaaagttg tcgaaggtaa ccgtgaacgt atccagattt ttgaaggtgt
     5161 tgttatcgca cgtaaaggtg ctggaatctc agaaaactac acagttcgta aaatctctaa
     5221 cggtgtaggt gttgggcgta tcttcccaat ccacactcca cgtgttgaaa aaatcgaagt
     5281 tgttcgttac ggtaaagtac gtcgtgcgaa attgtactac ttgcgtgctc ttcaaggtaa
     5341 agcagctcgt atcaaagaaa tccgtcgtta attcgactaa aaaactctgc ttttcagcag
     5401 agtttttatc tagctccctt agttcaatgg atataacaac tccctcctaa ggagtagttg
     5461 caggttcgat tcctgcaggg ggcagtaatt agacatcaaa aaccgctcaa ctgagcggtt
     5521 ttagtctatt ctttcctttt acagtaccca tgtacgaata gcatgggcaa cgccggattc
     5581 gtcatttgtt ttggtgatgt atttggcgat ttttttgatt tctggatttc cattttccat
     5641 gacaacgggg tttccaacga cttccagcat ggcacggtca ttttcttcgt ctccgatagc
     5701 catggtttca tctttggtca atccgagttt ttcagccaag tgagtaatgg ctgaaccctt
     5761 gtctacattc tttgtaaaga gttcgaggta gaaaggagca gatttgttga tggagtagcg
     5821 ctcgtaaaat tctgctggaa ttttttcaat cgcagcatcg agaatttctg gttcatcgat
     5881 aaacatacat ttaacaattt ctttgccagc catttcttca ggggtacggt agaagatagg
     5941 catgctgacg agggttgatt cgtgtacagt gtattttccg atattgcgat ttgcagtata
     6001 gataccgtcc ttggtaatgg catgcatgtg gacaccgagc ttgcgactga ggaattccat
     6061 atccagataa tcctcatagg tcaaggattc gctgataatc tcatggccag tagcagtttc
     6121 ttggacaagg gcaccattga aggtcaccac atagtcacct tggtctctca actgcaagtc
     6181 gtccagaagt ttggcaacac ctgcgatggg gcggccagtt gcaatcacga ctttgacacc
     6241 agcttctttg gcatcttgga tggcagaaaa gacttcagga gtgatttcct tttggctgtt
     6301 gactagggtt ccgtcaatat cgacggcaat tagtttaata ctcataaatt tcctcttcta
     6361 gtttttattt ggggtaaaat gatcgttctc aatcaagtgt aaaaattgct gggtaatgct
     6421 tgcgaagatg ctgttttggt ctaacatttc tttagggaaa tagaaacgat tatctccgtg
     6481 gcgactgcca gcaagggatt ggacgatagg agacaggcta gagagttcgg ccagttctcc
     6541 atttttttgt aaaatctcaa tctgtgtccg tgggttttca gattcgggac gatagatatc
     6601 ataagggagg tcaaagttct tatgaatggc agtgtagtag tcgggatcaa agccgatatc
     6661 ctcagctaat tttctcatgc tggcgagttg gtcttggttc tcttgtgaaa aggtaatgga
     6721 tttaaagacc ttgcggttga caaagcgatg cgataaatct gcaagaatct tgtcaggact
     6781 ggtcatccaa agctggaagt aggtattcat cacgccatca tccagagcca gatagtcagt
     6841 caaggtcaca tttttttcga agaaaggcag gaggtgtgga gaagttcggg caaagaaatc
     6901 cttgtcctca ggatagagtt ccttggcgcg tttgagaaga ttctgtagga gaacttccat
     6961 ggcgcgtgtt gcggggtgga aataaacctg catgtacatc tggtagcgac tgaggacgta
     7021 gtcttcgatg gcgtgcatgc cattgcgctg aaaggcgata ccattttcga taggacgaat
     7081 gactcggagg attcgagtca ggtcaaattc cccataggat gctcctgtaa aataggagtc
     7141 gcgcaagaga tagtccatgc gatctgcgtc aatctgacta gaaatgagct gcacgacctg
     7201 cttattagga taggtatggt caatgacact ggccaccttt tctgggaaat caggtgccac
     7261 ttgtagcagg acttggtgaa tctctgtctc aggattttga ataatctcct gagtaatggc
     7321 ttcatggtct gtatcaaaga gattttcaaa agtatgggag taggcaccat gcccaaggtc
     7381 gtgtaggaga gcagcggtca tggtcaagag agactcggca ggattccatt cctcaggata
     7441 tttttcttcg aaaatctctg tgatgcgtcg tgcaatttca tagactccta gacagtgaga
     7501 gaagcgactg tgttctccac cgtggaaggt ataactggaa gttcccagtt gtttgatccg
     7561 gcgcaaacgc tgaaattctt ttgtattaat caagtcatag atgatttgat tattgacatg
     7621 gatgtagttg tgaacagggt cacggaatac tttttcgttc atatgaccat tatagcaaaa
     7681 tcctgctctt tttggtaaaa tagtaggaca agagacaaga aagaggacct gatgtgaaga
     7741 ttcggatgcg aaatacaatt cagtttgatg agcagttgga agtgattgac cagctttatg
     7801 atgtggaagt gcatgaaaaa ggagattata gctacctgct tttctataat gaggaaaagg
     7861 aaaaagtagt gattaaattt catggtcaag aacttgtgat gagtcgattt tccaatccca
     7921 agaccattat gcgctttcta aaggatagtg atagtttagc ctatattccc acacctatgg
     7981 gtatgcagga gtttattatc caaacgaatc gttatcaagt ggatgggcaa aagattgaac
     8041 tagattatca actacaaaat caagagggac atccctttgc cagctatcaa ttggaaatta
     8101 cttggggcta ggctcatgtc tttttcaaat ttagctatct atcttcttgg atcggcttcc
     8161 tagtgtggta gattagggat aatatccaaa tttcatacag cagttatatt gttatgtaca
     8221 cagagattag ttttgtacta gtctctttct ctttatttta ccgaagacta ttggttggat
     8281 aatcctctca aatatggtat actcattact gtaaaaggag atggatgcct agatgctgaa
     8341 aacacaagaa attgccgata aatatggtat aacaagacag acattgaata attggatgca
     8401 aaaaggaata atttctcaac ctcgaaaaaa taatcgttct gcttatgaat gggaagaaga
     8461 aaatgaaaaa gagattgtga gagtgatttc agaagaaatt cctgcaaaat attatgtttc
     8521 aaataaagag acattaaaaa ttggaaatcg acgctatcta ggaagtaaac aaaagatgct
     8581 tgatttcata ttgaagacag tatcagaaaa tattggaagt atcgatagtg tggcggatat
     8641 atttggtgga acaggtgttg ttgcagactt atttagaaaa cagaataaaa aagttatagt
     8701 gaatgatata ctctatagca actttatttc ctttcaaact tggttttcga atgaggatgt
     8761 agatattcat aaagtttctc atataattga tgaattaaat aatttatccc ctaaaaaagg
     8821 ctatgtttct aagaattttg gtggtgctta cttttctgaa gaaaatgctg gaaagattga
     8881 ctcaattcgt gaagaaattg aaaaatataa atcaggtaat cagagagagt atttcatgtt
     8941 actaaccagt ctattgtacg ctatggataa agtggctaat actgtaggtc attatgacgc
     9001 ttataggaaa aaaatggatt attgcaagga aatatattta agagtccctg agtataatga
     9061 aaacaatcag aatgaaattt ataataaaga tgccaataaa ttagttaaag aaatttatgc
     9121 agatcttgtt tatattgata ctccatataa ttcccgtggc tatgaaaatg cctaccatgt
     9181 tttagaaaat attgctgaat ggaaaaaacc agatgtagaa ggtgtagcga aaaaagcagt
     9241 taatcgttca gaaaaagggt cggattatac aaaaagtaaa gctcctcaag cttttgaaga
     9301 tttaattcta aacattaatg ctaagtatat attggttagc tataacaata tgaataaaaa
     9361 aggtaattcc cgttcaaatg ctaagatttc aaacgaagaa ataattaaga ttttatcaaa
     9421 acgaggtaag gttcaagtat ttgaaactga tttctcacct ttcacaactg gtaagtctaa
     9481 aattgaaaac cacaaggaat tgctatattt atgcataatt tctccagaga aaaaaaaaga
     9541 aaaaaagtta attaagagcg ctcttaacta tactggagga aagtataaat tactccctca
     9601 gttattacct ctttttcctg aaagctacaa taattttatt gatttattct ctggtggagc
     9661 aacggtggca gttaatcttg ctaatattaa taaatcaaaa acgaaaaagt atatcatcaa
     9721 tgatataagt gaggaagtaa ttgattttta taaatatttg gaaagtcaaa aagatgtaac
     9781 ggtatttttg gataaagtag agaaagctat agaattttat aaactttcga acacacagaa
     9841 atatagctat gctcactacg gtgtaaatag ctctacagga ctggggtctt ataat
//
LOCUS       DAVKWZ010000065         9808 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000065 DAVKWZ010000000
VERSION     DAVKWZ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9808
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.065"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            108..584
                     /locus_tag="VW854_001799"
     CDS             108..584
                     /locus_tag="VW854_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV0262995.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEHLFDQVATLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAEATA"
     gene            622..927
                     /locus_tag="VW854_001800"
     CDS             622..927
                     /locus_tag="VW854_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV0262996.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            975..2450
                     /locus_tag="VW854_001801"
     CDS             975..2450
                     /locus_tag="VW854_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEV0262997.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            2476..2547
                     /locus_tag="VW854_001802"
                     /pseudo
     CDS             2476..2547
                     /locus_tag="VW854_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            2845..9546
                     /locus_tag="VW854_001803"
     CDS             2845..9546
                     /locus_tag="VW854_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEV0262998.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASSQEKPLKEDAKAVTNEEVNQMIEDRKVDFNQNWYF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLGTEVARVRTLIGKEPKMPKTVGFVYSDGSREKLPVTWSQ
                     VDVSQAGVVTVKGTANGREVEAHVEVLAIAKELPTVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEITEVDKAKLSVAGSRIQMTGQLAGETIHATLVVEEGNAAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFVQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLNAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQEPSLPATVTVEYDKGFPKAHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRSNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAIRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVPAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLTVEWEEVPAEN
                     LTKAGQFTVRGRVLGSNLVAEVTVRVTDKLGETLSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQGKVQFFA
                     DSGTDAPSKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVHYRADKDIEAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IHLTKEKPVSEKTVTAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGK
                     ERIFTAINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
BASE COUNT         3167 a         1866 c         2144 g         2631 t
ORIGIN      
        1 aacaaaaata taaaaaaaga gacaaaaaag aacagaaagc aattgacaac ggtttcatat
       61 agtgttatac ttatggcata aagttaatgg aaagaaggga aaaacttatg gtattagatt
      121 atttctttga caaaaacctt gtgttttgct tagaagcgga taatcaagaa catctctttg
      181 atcaggttgc aactttattg gaagaacgag aaattgtaac tccaacttat cgtgaagcct
      241 tgatcacgcg tgaaaagtca tttccaactg gtttagatat ggaatttcta ggaaaggact
      301 tgccaaatgt agcgattcct catacagata ttgttcataa tctagctgag aaagtggtgg
      361 ttgttcgatt agaaaaacca gtaacttttc acaatatgat agctcctgat aaggaagtag
      421 aagtatccct acttttcttt atcattaaca attcaagttc aagtcaaaca aatattctgg
      481 ctcagttgat ggactttttc acaggaaatg gacatcttga agacctatca aaaatttccg
      541 aaccagaaaa actttatgct tacattgctg aagcaaccgc ttaatcttgt ctattaaaaa
      601 ataaaatcgg aggaaatcca aatgattaaa attcttgctg cctgcggtgc aggtgttaac
      661 tcaagtcacc aaattaaaag tgctctagaa gaagaacttt caaaccgtgg ttatgatgtt
      721 cactgtgatg cagtcatggt gaaagatgta aacgaagacc ttatgaaagg ttacgatatc
      781 tttacaccaa tcgctgcaac agaccttggt tttgaaccag gtatcccagt tatcgaagct
      841 gggccaatct tattccgtat cccagcaatg agcgctccag tatttgacaa tattgaagca
      901 gctattaaag aacatggatt aagctaattc taatttgtta acattcatat aacataaaaa
      961 agggaggaac tgttatggat gcaatctttg acctaatcgg aaaggttttc aatcccatct
     1021 tagaaatggg tggacctgtc atcatgttga tcattttgac agtattggct ttactttttg
     1081 gagtgaaatt ctccaaagcg cttgaaggtg gtatcaaact tgccatcgct cttacaggta
     1141 tcggtgctat catcggtatg ctaaatggtg ctttctcagc atcacttgca aaattcgttg
     1201 aaaacactgg tatccaattg agtattacag acgttggttg ggcaccactc gcaacaatca
     1261 cttggggttc tgcttggaca ctatacttct tgctcatcat gttgattgtc aacgtagtga
     1321 tgcttgctat gaagaaaacc gatacacttg atgtcgatat ctttgatatc tggcacttgt
     1381 ctatcacagg tctcttgatt aaatggtatg ctgataacaa tggtgtgagt caaggggttt
     1441 cactctttat tgctacagca gctatcgtcc ttgtcggtgt gttgaaaatt atcaactctg
     1501 acttgatgaa acctacattt gatgaccttc ttaacgctcc aagttcatca ccaatgacat
     1561 caactcacat gaactacatg atgaacccag ttatcatggt tttggataag atttttgaaa
     1621 aattcttccc aggccttgat aaatatgact ttgatgctgc taaattgaac aagaaaatcg
     1681 gtttctgggg atctaaattc ttcatcggtt tcatccttgg tatcgttatc ggtattatgg
     1741 gaactccaca tccaattgca ggtgttgcag atgcagataa atggcgtctt gttatcaaag
     1801 gatggttgtc tcttggtttg actgccggtg tatctttgga actcttctca cttatcggtt
     1861 catggttcat cgcagccgta gaaccactat cacaaggtat tacaaacgtt gctactaaac
     1921 gtcttcaagg acgtaaattc aatatcggtc ttgactggcc attcatcgct ggtcgtgctg
     1981 aaatctgggc ttgtgccaac gtacttgcac caatcatgtt gattgaagca gtgcttcttt
     2041 caaaagttgg aaatggtatc ttgccacttg caggtatcat cgctatgggt gttactccag
     2101 ctctcttggt tgtaactcgt ggtaaattgc tccgtatgat tatcttcgga acactcttgt
     2161 tgccactctt ccttctttca ggtacactta ttgcaccatt tgcaacagaa cttgctaaag
     2221 gtgtaggtgc cttcccagaa ggtgtgagcc aaactcaatt gattactcac tctactcttg
     2281 aaggaccaat cgaaaaactt cttggttgga caattggtaa cactacaact ggtgatatca
     2341 aagcaatcct tggtgcagta gtcttccttg tattctatat cggtatcttt gcttggtaca
     2401 gaaaacaaat gatcaaacgt aacgaagagt acgcagcaaa agcaaaataa tgcgctccta
     2461 taaaatataa acttaatgaa aatcaaagag caaactagga agctagccgc aggttgctca
     2521 aaacacagtt ttgaggttgt agataagact gacgaagtca gctcaaaaca tggttttgag
     2581 gttgtagata agactgacga agtcagctca aaacatggtt ttgaggttgt agatgaaact
     2641 gacgaagtca gctcaaaaca tggttttgag gttgtagatg aaactgacga agtcagtaat
     2701 catatctacg gcaaggcgac actgacgaag tttgaattgg attttcgcag agtgtaaatt
     2761 gttgaaacta ggttgtcata ccatgtatac cacttgggct ttggtagtag ctaactgcac
     2821 taaatataat ataaggaggg ttttatgggg aaaggccatt ggaatcggaa aagagtttat
     2881 agcattcgta agtttgctgt gggagcttgc tcagtaatga ttgggacttg tgcagttcta
     2941 ttaggaggaa atatagctgg agaatctgta gtttatgcgg atgaaacact tattactcat
     3001 actgctgaga aacctaaaga ggaaaaaatg atagtagaag aaaaggctga taaagctttg
     3061 gaaactaaaa atgtagttga aaggacagaa caaagtgaac ctagttcaac tgaggctatt
     3121 gcatctgaga agaaagaaga tgaagccgta actccaaaag aggaaaaagt gtctgctaaa
     3181 ccggaagaaa aagctccaag gatagaatca caagcttcaa gtcaagaaaa accgctcaag
     3241 gaagatgcta aagctgtaac aaatgaagaa gtgaatcaaa tgattgaaga caggaaagtg
     3301 gattttaatc aaaattggta ctttaaactc aatgcaaatt ctaaggaagc cattaaacct
     3361 gatgcagacg tatctacgtg gaaaaaatta gatttaccgt atgactggag tatctttaac
     3421 gatttcgatc atgaatctcc tgcacaaaat gaaggtggac agctcaacgg tggggaagct
     3481 tggtatcgca agactttcaa actagatgaa aaagacctca agaaaaatgt tcgccttact
     3541 tttgatggcg tctacatgga ttctcaagtt tatgtcaatg gtcagttagt ggggcattat
     3601 ccaaatggtt ataaccagtt ctcatacgat atcaccaaat accttcacaa agatggtcgt
     3661 gagaatgtga ttgctgtcca tgcagtcaac aaacagccaa gtagccgttg gtattcagga
     3721 agtggtatct atcgtgatgt gactttacaa gtgacagata aggtgcatgt tgagaaaaat
     3781 gggacaacta ttttaacacc aaaacttgaa gaacaacaac atggcaaggt tgaaactcat
     3841 gtgaccagca aaatcgtcaa tacggacgac aaagaccatg aacttgtagc cgaatatcaa
     3901 atcgttgaac gaggtggtca tgctgtaaca ggcttagttc gtacagcgag tcgtacctta
     3961 aaagcacatg aatcaacaag cctagatgcg attttagaag ttgaaagacc aaaactctgg
     4021 accgttttaa atgacaaacc tgccttgtac gaattgatta cgcgtgttta ccgtgacggt
     4081 caattggttg atgctaagaa ggatttgttt ggttaccgtt actatcactg gactccaaat
     4141 gaaggtttct ctttgaatgg tgaacgtatt aaattccatg gagtatcctt gcaccacgac
     4201 catggggcgc ttggagcaga agaaaactat aaagcagaat atcgccgtct caaacaaatg
     4261 aaggagatgg gagttaactc tatccgtaca acccacaacc ctgctagtga gcaaaccttg
     4321 caaatcgcag cagaactagg tttactcgtt caggaagagg cctttgatac gtggtatggt
     4381 ggcaagaaac cttatgacta tggacgtttc tttgaaaaag atgccactca cccagaagct
     4441 cgaaaaggtg aaaaatggtc tgattttgac ctacgtacca tggtcgaaag aggcaaaaac
     4501 aaccctgcta tcttcatgtg gtcaattggt aatgaaatag gtgaagctaa tggtgatgcc
     4561 cactctttag caactgttaa acgtttggtc aaggttatca aggatgttga taagactcgc
     4621 tatgttacca tgggagcaga taaattccgt ttcggcaatg gtagcggagg gcatgagaaa
     4681 attgctgatg aactcgatgc tgttggattt aactattctg aagataatta caaagccctt
     4741 agagctaagc atccaaaatg gttgatttac ggttcagaaa catcatcagc aacccgtaca
     4801 cgaggaagtt actatcgccc tgaacgtgaa ttgaaacata gcaatggacc tgagcgtaat
     4861 tatgaacagt cagattatgg aaatgatcgt gtgggttggg ggaaaacagc aaccgcttca
     4921 tggacttttg accgtgacaa cgctggctat gctggacagt ttatctggac aggtacggac
     4981 tatattggtg aacctacacc atggcacaac caaaatcaaa ctcctgttaa gagctcttac
     5041 tttggtatcg tagatacagc cggcattcca aaacatgact tctatctcta ccaaagccaa
     5101 tgggtttctg ttaagaagaa accgatggta caccttcttc ctcactggaa ctgggaaaac
     5161 aaagaattag catccaaagt agctgactca gaaggtaaga ttccagttcg tgcttattcg
     5221 aatgcttcta gtgtagaatt attcttgaat ggaaaatctc ttggtcttaa gactttcaat
     5281 aaaaaacaaa ccagcgatgg gcggacttac caagaaggtg caaatgctaa tgaactttat
     5341 cttgaatgga aagttgccta tcaaccgggt accttggaag caattgctcg tgatgaatct
     5401 ggcaaggaaa ttgctcgaga taagattacg actgctggta agccagcggc agttcgtctt
     5461 attaaggaag accatgcgat tgcagcagat ggaaaagact tgacttacat ctactatgaa
     5521 attgttgaca gccaggggaa tgtggttcca actgctaata atctggttcg cttccaattg
     5581 catggccaag gtcaactggt cggtgtagat aacggagaac aagccagccg tgaacgctat
     5641 aaggcgcaag cagatggttc ttggattcgt aaagcattta atggtaaagg tgttgccatt
     5701 gtcaaatcaa ctgaacaagc agggaaattc accctgactg cccactctga tctcttgaaa
     5761 tcgaaccaag tcactgtctt tactggtaaa aaagaaggac aagagaagac tgttttgggg
     5821 acagaagtag caagagttcg tacattaatt ggtaaagaac caaaaatgcc gaaaacagta
     5881 ggttttgtat acagcgatgg tagtcgtgaa aaacttccag taacttggtc tcaagtcgat
     5941 gttagtcaag caggagttgt gactgttaaa ggtacagcaa atggacgtga ggtagaggct
     6001 catgtcgagg ttctagcaat tgcgaaagaa ttaccaacag ttaaacgtat tgctccaaat
     6061 actgacttga attctgtaga caaatctgtt tcttatgttt tgactgatgg aagtgtacaa
     6121 gagtacgaag tagatagctg ggagattacg gaagtagata aagccaaact ttcagtagct
     6181 ggatcacgta ttcaaatgac tggtcagtta gctggagaaa ctattcatgc aacccttgtg
     6241 gtagaagaag gaaatgctgc agcacctgta gtgccaactg ttactgttgg aggtgaagca
     6301 gtaacaggtc ttactagtcg acaaccaatg caatatcgta ctctatctta tggtgcccaa
     6361 ttgccagaag tcacagcaag tgctgaaaat gctgatgtga cagttcttca agcaagcgca
     6421 gcaaacggca tgcgtgcaag catatttgtt cagcctaaag atggtggccc tcttcaaacc
     6481 tatgcaattc aattcctaga agaagcacca aaaattgctc acttgagctt gcaagtggaa
     6541 aaagctgaca gtctcaaaga agaccaaact gtcaaattgt cggttcgagc tcactatcaa
     6601 gatggaacgc aagctgtatt accagctgat aaagtaacct tctctacaag tggtgaaggg
     6661 gaagtcgcaa ttcgtaaagg aatgcttgag ttgcataagc caggagcagt cactctgaac
     6721 gctgaatatg agggagctaa aggccaagtt gaactcacta tccaagccaa tactgagaag
     6781 aagattgcgc aatccatccg tcctgtaaat gtagtgacag atttgcatca ggaaccaagt
     6841 cttccagcaa cagtaacagt tgagtatgac aaaggtttcc ctaaagctca taaagtcact
     6901 tggcaagcta ttccgaaaga aaaactagac tcctatcaaa catttgaagt actaggtaaa
     6961 gttgaaggaa ttgaccttga agcgcgtgca aaagtctctg tagaaggtat cgtttcagtt
     7021 gaagaagtca gtgtgacaac tccaatcgca gaagcaccac aattaccaga aagcgttcgg
     7081 acatatgatt caaatggtca cgtttcatca gctaaggttg catgggatgc gattcgtcca
     7141 gagcaatacg ctaaggaagg tgtctttaca gttaatggtc gcttagaagg tacgcaatta
     7201 acaactaaac ttcatgttcg cgtatctgct caaactgagc aaggtgcaaa catttctgac
     7261 caatggaccg gttcagaatt gccacttgcc tttgcttcag attcaaatcc aagcgaccca
     7321 gtttcaaatg ttaatgacaa gctcatttcc tacaataacc aaccagccaa tcgttggaca
     7381 aactggaatc gtagtaatcc agaagcttca gtcggtgttc tgtttggaga ttcaggtatc
     7441 ttgagcaaac gctccgttga taatctaagt gtcggattcc acgaagacca tggagttggt
     7501 gcaccgaagt cttatgtgat tgagtattat gttggtaaga ctgtcccaac agctcctaaa
     7561 aaccctagtt ttgttggtaa tgaggaccat gtctttaatg attctgccaa ctggaaacca
     7621 gttactaatc taaaagcccc tgctcaactc aaggctggag aaatgaacca ctttagcttt
     7681 gataaagttg aaacctatgc tattcgtatt cgcatggtta aagcagataa caagcgtgga
     7741 acgtctatca cagaggtaca aatctttgcg aaacaagttg cggcagccaa acaaggacaa
     7801 acaagaatcc aagttgacgg caaagactta gcaaacttca accctgattt gacagactac
     7861 taccttgagt ctgtagatgg aaaagttccg gcagtcacag caagtgttag caacaatggt
     7921 ctcgctaccg tcgttccaag cgttcgtgaa ggtgagccag ttcgtgtcat cgcgaaagct
     7981 gaaaatggcg acatcttagg agaataccgt ctgcacttca ctaaggataa gaacttactt
     8041 tctcataaac cagttgctgc ggttaaacaa gctcgcttgc tacaagtagg tcaagcactt
     8101 gaattgccga ctaaggttcc agtttacttc acaggtaaag acggctacga aacaaaagac
     8161 ctgacagttg aatgggaaga agttccagcg gaaaatctga caaaagcagg tcaatttact
     8221 gttcgaggcc gtgtccttgg tagtaacctt gttgctgagg tcactgtacg agtgacagac
     8281 aaacttggtg agactctttc agataaccct aactatgatg aaaacagtaa ccaggccttt
     8341 gcttcagcaa ccaatgatat tgacaaaaac tctcatgacc gcgttgacta tctcaatgac
     8401 ggagatcatt cagaaaatcg tcgttggaca aactggtcac caacaccatc ttctaatcca
     8461 gaagtatcag cgggtgtgat cttccgtgaa aatggtaaga ttgtagaacg gactgttgca
     8521 caaggaaaag ttcagttctt tgcagatagt ggtacggatg caccatctaa actcgtttta
     8581 gaacgctatg tcggtccaga gtttgaagtg ccaacctact attcaaacta ccaagcctac
     8641 gacgcagacc atccattcaa caatccagaa aattgggaag ctgtgcatta tcgtgcggat
     8701 aaagacattg aagccggtga tgaaattaac gttaccttta aagctgtcaa agccaaagcc
     8761 atgagatggc gtatggagcg taaagctgac aagagcggtg ttgcgatgat tgagatgacc
     8821 ttcctggcac cgagtgaatt gcctcaagaa agtacgcaat cgaagattct tgtagatgga
     8881 aaagaacttg ctgatttcgc tgaaaatcgt caagactatc aaattaccta taaaggtcaa
     8941 cggccaaaag tctcagttga agaaaataat caagtagctt caactgtggt agatagtgga
     9001 gaagatagcc ttccagtact tgttcgcctc gtttcagaaa gtggaaaaca agtcaaggaa
     9061 taccgtatcc acttgactaa ggaaaaacca gtttctgaga agacagttac tgctgtacaa
     9121 gaagatcttc caaaactcga atttgttgaa aaagatttgg cctacaagac agttgagaaa
     9181 aaagattcaa cactgtatct aggtgaaact cgtgtagaac aagaaggaaa aactggtaaa
     9241 gaacgtatct ttacagcgat taatcctgat ggaagtaagg aagaaaaact ccgtgaagtg
     9301 gtagaagctc cgacagaccg catcgtcttg gttggaacca aaccagtagc tcaagaagct
     9361 aaaaaaccac aagtgtcaga aaaagcagat acaaaaccaa ttgattcaag tgaagctagt
     9421 caaactaata aagcccagtt accaaataca ggcagtgcgg caagccaagc agcagtagca
     9481 gcaggtttag ctcttctagg tttgagtgca ggattagtag ttactaaagg taaaaaagaa
     9541 gactagaaat tttgatacct tctttatcat taaaggccaa agaaaaactc ttgtcctaaa
     9601 tagaagataa agagtctggg acaaaatagt tctcaaccac aaaaaagcta gatatttcca
     9661 attgtggaac tctagctttt taattttgag tcattctctt attgtatagt ggattgaaac
     9721 tagaatagta cagttttact tctaaaacat tgttagaaat cgatttgact gtccttatcg
     9781 atttgtcctg ttcttatttc attttact
//
LOCUS       DAVKWZ010000066         9805 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000066 DAVKWZ010000000
VERSION     DAVKWZ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9805
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.066"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            14..493
                     /gene="rlmH"
                     /locus_tag="VW854_001804"
     CDS             14..493
                     /gene="rlmH"
                     /locus_tag="VW854_001804"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEV0262999.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            537..610
                     /locus_tag="VW854_001805"
     tRNA            537..610
                     /locus_tag="VW854_001805"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:571..573,aa:Arg,seq:tct)
     gene            775..900
                     /gene="comC"
                     /locus_tag="VW854_001806"
     CDS             775..900
                     /gene="comC"
                     /locus_tag="VW854_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HEV0263000.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRLSKFFRDFILQRKK"
     gene            921..2246
                     /locus_tag="VW854_001807"
     CDS             921..2246
                     /locus_tag="VW854_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV0263001.1"
                     /translation="MDLFGFGTVIVHFLIISHSYHFICKGQINRKELFVFGAYTLLTE
                     IVFDFPLYILYLDGLGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDEDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            2243..2995
                     /locus_tag="VW854_001808"
     CDS             2243..2995
                     /locus_tag="VW854_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0263002.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            3047..3118
                     /locus_tag="VW854_001809"
     tRNA            3047..3118
                     /locus_tag="VW854_001809"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3080..3082,aa:Glu,seq:ttc)
     gene            3124..3197
                     /locus_tag="VW854_001810"
     tRNA            3124..3197
                     /locus_tag="VW854_001810"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3158..3160,aa:Asn,seq:gtt)
     gene            complement(3238..3780)
                     /locus_tag="VW854_001811"
     CDS             complement(3238..3780)
                     /locus_tag="VW854_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEV0263003.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            3958..5519
                     /locus_tag="VW854_001812"
                     /pseudo
     CDS             3958..5519
                     /locus_tag="VW854_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518194.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
     gene            complement(5549..8101)
                     /locus_tag="VW854_001813"
     CDS             complement(5549..8101)
                     /locus_tag="VW854_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEV0263004.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAIYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLFPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVAGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEVIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(8163..9785)
                     /locus_tag="VW854_001814"
     CDS             complement(8163..9785)
                     /locus_tag="VW854_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV0263005.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
BASE COUNT         3280 a         1512 c         1823 g         3190 t
ORIGIN      
        1 ttagaataga gtcatgaaaa ttaaagttgt aacagttggg aaactgaaag aaaagtattt
       61 aaaagatggt atcgcagagt attcaaaacg aatttctaga tttgctaagt ttgaaatgat
      121 tgagttatca gatgaaaaaa caccagataa ggccagtgaa tcagaaaatc aaaagatttt
      181 agaaatagaa ggtcagagaa ttttatcaaa aattgctgac cgtgatttcg ttattgtgtt
      241 agccattgaa gggaaaactt tcttctcaga agaatttagt aagcagttag aagaaacttc
      301 tataaaagga ttttctactc ttacttttat tattggggga agtttaggat tgtcatcatc
      361 tgtaaaaaat agagccaatc tttctgtcag ttttggtcgc ctaaccttac cccatcagtt
      421 aatgagacta gttcttgttg aacaaatcta tcgcgctttt acgattcagc agggattccc
      481 ctaccataaa tagaaaattg acttttaatt gaatttttgg tagaataatt gtattaggtc
      541 tcatagctca gctggataga gcattcgcct tctaagcgaa cggtcgcagg ttcgaatcct
      601 gctgggatca atataatagc aaagctggga attttcccgg cttttttctt aaaaaagtac
      661 actttgggag aaaaaaatga tagttgagag aattttatct aaaacgaaat tccattttgt
      721 ataatggttt ttgtaagtta gcttacaaga aaaaacattt taggagattt tattatgaaa
      781 aacacagtta aattggaaca gtttgtagct ttgaaggaaa aagacttaca aaagattaaa
      841 ggtggggaga tgaggttgtc aaaattcttc cgtgatttta ttttacaaag aaaaaagtaa
      901 tgaaataagg ggaaagagta atggatttat ttggatttgg gacggttatt gttcattttt
      961 taattattag tcacagttac cattttattt gtaaaggtca aataaataga aaagaattat
     1021 tcgtttttgg tgcttataca ttactaacag aaatagtatt tgattttccc ttatatattc
     1081 tatatttaga tgggttaggg attgaaagat ttttatttcc tttgggatta tattcctatt
     1141 ttcgatggat gaaacagtat gagagggata gaggactatt cctaagttta ctactatctc
     1201 ttttatatga gagcactcat aactttctgt ccgtaacttt ctcctctata acaggagata
     1261 attttgtttt acaatatcat ttcccattct ttttcgttgt gacggtgtta acctattttg
     1321 ttacattaaa aatcatttac tatttccatt tggaactagc ctattttgac gaggactacc
     1381 tttatccttt cttgaaaaaa gtattttttg ctttactatt gctacatatt gtatctttcg
     1441 tttcagatat ggtaagtacg attaaacatt tgaatagttt tggaagtatt ttgtcatcta
     1501 ttgtctttat ctctctcctt ttgaccttct ttgcaatgaa ttctcataaa gttcaaatgg
     1561 agaaagagat tgctttgaag cagaagaaat ttgaacagaa acatttacag aattacacag
     1621 atgaaattgt tggtctgtat aatgaaatcc gtggttttcg acatgattat gctggaatgc
     1681 ttgtcagcat gcagatggca attgacagtg gtaatttaca ggaaattgac agaatttaca
     1741 atgaagtttt agtcaaagca aatcataaat tgcgttcaga taagtacact tactttgatt
     1801 tgaacaacat agaagactca gctttacgaa gtttggttgc tcagtcaatt gtctatgctc
     1861 gaaataatgg tgtagagttt acactggaag taaaagatac gattaccaag cttccaattg
     1921 aactattgga tttggttcgt atcatgagcg ttttattgaa taatgctgtc gaaggatcgg
     1981 ctgatagcta taaaaagcag atggaagtag cagttattaa gatggaaact gaaacagtta
     2041 ttgtgattca gaattcatgt aaaatgacga tgactccttc aggagatcta tttgccttag
     2101 gattctccac taagggaaga aatcgcggag tcggattaaa taatgtgaaa gaactactag
     2161 ataagtacaa caatattatt ttagaaacag agatggaagg cagtacattt agacaaatca
     2221 ttagatttaa gagggaattt gaatgaaagt tttaatttta gaagatgtta ttgaacatca
     2281 agtgagacta gagagaatat tggatgaaat ttcgaaagaa tcgaatattc caatatcata
     2341 caagacaacg ggaaaagtcc gtgaatttga agaatacatt gaaaatgatg aagtaaatca
     2401 gctttatttc ctagatatcg atattcatgg aattgagaaa aagggatttg aagtggctca
     2461 gctcattcgt cattacaatc cttacgctat tatcgtcttt atcactagtc gatcagagtt
     2521 tgcgactcta acctataaat accaggtatc agccctagat tttgttgata aggatatcaa
     2581 tgatgagatg tttaagaaga gaattgagca aaatatcttc tacacgaaga gtatgttact
     2641 tgaaaatgaa gatgttgtag attatttcga ctacaattac aagggaaatg atttaaaaat
     2701 tccttaccat gatattttgt atattgaaac aacaggggtc tctcataaat tgcgcattat
     2761 tggtaagaat tttgcaaaag agttttatgg taccatgaca gatattcagg aaaaggacaa
     2821 acatactcag cgattttatt ctcctcacaa gtcatttttg gtaaatatag gcaatatcag
     2881 agaaattgat cgaaaaaact tagaaattgt tttctatgaa gaccatcgtt gtcctatttc
     2941 aagattaaaa attagaaaat taaaagatat tttagagaaa aaatctcaaa agtgattgac
     3001 aattagcaag aaattgatat aatggttata tctgctacag atagaaggtc cgttggtcaa
     3061 ggggttaaga caccgccttt tcacggcggt aacacgggtt cgaatcccgt acggactatt
     3121 ttatccgcca tagctcagtt ggtagtagcg catgactgtt aatcatgatg tcgtaggttc
     3181 gagtcctact ggcggagtat agataaaagg gaacacagct gtgttcctct ttttgtatca
     3241 atttgtatca ccaagcattt tcataaggaa gtctgttatt tcttgagaac tttctttttt
     3301 tccatgtgca atccaagttt ggcagacacc aaaaagtgca tgagttagat agatgctact
     3361 atattctaat tcggtggtat ttagattcag ttgcataaat cgcttttgta aatctgtact
     3421 aagcatgata tgaagtttat ttcgtaagaa attttggatt tctttagtcc cattttcaga
     3481 aagaagggca gccagaagtg gttctgactc tagatattca aaaacttcta aaatagcgtc
     3541 tcttttgtga tgagcatgtt tttgaaaaat atattcaaat gtatggaata gcttgctttg
     3601 atagtgctca atcatatcat acttatcctt atagtgagta tagaagctgg aacgactaat
     3661 tccggctttt tctactaatt tgacagtaga aattttatca aatggctgtt ccatcagtaa
     3721 ttgtaccata gcattttcaa tagttcgctt tgtttttaag cgtttgttac tttcttgcat
     3781 atttcctcct tgtaaacaaa ttagactata tgtctaaaaa tagatttttt atcttgtaat
     3841 ttagattttt taatgtataa tctattatat caaaatttta gacaatatgt ttaaaaaagg
     3901 agaaactaag tttaaagaat ggaaagcaat ttaaaaaaaa caacctttat tattgtcatg
     3961 atcgggattt ctcttattcc agctctgtac aatatcatat ttttgtcatt aatgtgggat
     4021 ccatatgggc aattgtctga cttacctgtg gcagttgtaa ataatgataa agaggcttcc
     4081 tataatggta atactatggc aataggaaaa gacatggtgt ccaatttaaa agaaaataaa
     4141 accttggatt ttcattttgt agatgaagag gaaggaaaga agggattgga agatggcgat
     4201 tactatatgg tagtgacttt accaagtgat ttatctaaaa aaacaactac attatccaat
     4261 attcaatcga cagcagctta tcaatcattg acaagtgagc aacaaactgg gataagtgat
     4321 tctgtatctc aaaattcaac tgatagtatt caatcggctc agtcaattgt agctttagta
     4381 caaggtttac agggaagttt agaaaactta caaaatcaat cttctaatct ttcgacttta
     4441 aaaaatcaag ctaatcaagt attacctatt acttctactt ctttgatagg attgtcaagt
     4501 ggattaacag agatacaagg agctgttact agcaaattag ttcctgccag tcagttgatt
     4561 gcatcaggtg taaacgcata tactacaggt gttgataaag tttctcaggg cgcaagtcaa
     4621 ctaagtgaaa aaaatgccac cttgacacaa aaatcttcta gattgacagc aggagttgat
     4681 aaattacaat caggatctgg gcaattagca gacaaatcca gtcagttact ttcaggtgct
     4741 tcttcattag agaatggagc taataaattg gcagatggat ctgggaaact agcagaaggt
     4801 ggaacaaagt taacttctgg attggaagat ttacagacag gacttgcttc tttaggacaa
     4861 ggactaggta atgctagtga tcaactcaaa tcagtatcaa cagaatctaa aaatgcagag
     4921 attttgtcaa atccactcaa tctttcaaaa acagacaatg atcaagttcc tgtaaatgga
     4981 atcgcaatag ctccttatat gatatcagtt gctctttttg ttgcagcaat atcaacaaat
     5041 atgatatttg cgaaattgcc ttcaggacgt catccagaga gccgttggcc ttggttgaaa
     5101 tctcgagctg aaataaatgg tattatagct gttttggcag gaattttggt atatggagga
     5161 gttcagctta ttggtttaac tgctaatcat gagatgagaa tatttattct catcatccaa
     5221 acaagtttag tattcatgtc tatggtgacc actttagcaa cgtggaatag ccgtatagga
     5281 gcttttttct cacttatttt gcttttacta tagttagcat caagtgcagg tacttatcca
     5341 cttgctttga caaatgattt ctttagatct attaatccct ggttaccaat gagctattca
     5401 gtttcgggat tacgacaaac aatctctatc aacaagtcat tttcctagct gtcatactag
     5461 ctctatttac tggtttaggt atgctagcct atcaacataa gaaaatggaa gaagattaaa
     5521 aaaatcgacc gattaactgg tcgatttttt atgccttaga tgactttcgt ctgtgattat
     5581 agattccaaa tagtaagaga gaagtaaagg aacagattgc tccagtaata aaaccattgg
     5641 gaatgaagga aagtgtaata gttcctttcc ccttgggaat gtcaactttc ataaatccag
     5701 tttgagcttg tttaatttct attttcttac catcttggta ggcagaccaa cctttgtcat
     5761 aaggaatggt gaagaaaata gatgtatctt gttggacatc atatgtagca aaaaccttgt
     5821 ttttagaagt tgatactgtg acaggttgtt ctttaatttt ttgaattacc tcggtgaaag
     5881 ttttggtatc taaacgatag aaggtaggag attcaaatga tacttgtgaa tttccaggga
     5941 aactaacatt gatattgaaa gtttttttct ctttagtata tcctagatta aagaaggaga
     6001 agacattatc agttgtaaaa gtcttttttt caccatttac aaggatgtca accttctttt
     6061 gtttatcgtt agaaaagtga aggtttatga aagagagata aacttggctg ttttctggaa
     6121 cttcaatttg atactggatt gctgcatctt catttgaaga acttgtgaca ctaatcaaat
     6181 cattagtatt ttctattttt tctgtttttt cataaggtat tggagaaaaa taatcaaaat
     6241 tgacgttagc aagttgattt aaaaatgagg cctgattatc caaggtatgt tcattgaact
     6301 tgacatcatt gtaaacagat tgactagcaa ctgcaatcgg aagagagtat tgattttcat
     6361 atagggtaag attatctttt tgatagatat ctttaaagcc atacttatca ataggactgt
     6421 ctgagatatt gtactggata ccaaataaac tatcagccaa aatactatta tttgcatatc
     6481 ggagattgag attagtccca gaggatttaa aaccaagttt atctaaagta gagcttgatg
     6541 aacgatttcg aacagatgaa aattgagaga ttccattgta gttgaatttc atactgtcat
     6601 ttcctgtctg agtttgtagt ttttcagtac gagtaaattg atttccaata tatgttgaga
     6661 aagattccat agctgggata tctcgactat aagcacttcg agaagcaaat ccccattcct
     6721 tagcaattcc gtccatttga gatgaagcat ttaaactcat ttcaaccagt ataaataaag
     6781 agattagaat ggcaaataga ttcacagata taaacttttt gataactgca aggagtaaaa
     6841 gagaatagac aaccaaaaat tcaagagtaa gcagaatatt caaatctgtt aaaaaagaat
     6901 aatgcgattt tagatagatg gtagctaaaa atcctgctac tacaagaaaa agcgaaacta
     6961 aaaaattcca gactttaagt tctttcagac gctttaagac ttctgctgct gtgtaaatta
     7021 acaaggtaga gaaaatccaa gcatagcgat gtaaaaacat gtttggagta tgcatgcctt
     7081 gccaaaataa gtcaagagct tctatgtaaa agcttgcaat tagaaatgca aagaatatta
     7141 catatatgag tttcacgtga aacttaatag atttcagcgt aaaaaataaa atggtcaaaa
     7201 taaagggaaa tagtccaaca aaaatcattg ggatggcccc atactttgtt gtgtcaaagg
     7261 aaccaatgaa ttgcttagca aagagatcaa gataccagct actttcagtt tgaaactttg
     7321 taacttcagt caatttttcc ccatgtgtct gtaaatcaaa tagagtggga agagtcataa
     7381 tcaaactagc cataccagct aaaaaggaga taactatgaa atcaagaaca gatgattttc
     7441 gagtcttaaa gtcccacgaa atttgacaga gataccagaa aataagaaac aatactgtca
     7501 tatatccaaa ataataattt tgaataaata agattgacag acttgtaaag tacaatagga
     7561 gtttcttttc agttatcagt agatgtaaac cagttataat taaaggaatc aagataaaaa
     7621 catctagcca ggtttttatc tctaattgac tgacagtgaa actcatcaga gcataggaag
     7681 tagataaggc tagttttaaa gtctgaggga tagattgaaa caatttattc aaactaaaaa
     7741 aggttgacag accaatcaat ccaaatttta agagagttgt cagatagata gcatctggca
     7801 tattcgttag atcaaaaaag taaaccagag gcgcgagaaa actacccaag taataactag
     7861 atagggcata gaagtttagc cctagaccac ttgtaaaggt gtaaaacaga ctactatttc
     7921 catgtaggat atttcgtaag gctacatcaa aaataacgta ttgatgaaag ccatctccta
     7981 atagaggaga gttgtcgcta ttccagtaga taccttgaga tagatatact ccagacataa
     8041 tcactacagg aatgatgaaa gaaataaaat aggttcgata tgtttttaaa aatgatttca
     8101 tgttacctcg tagaatgata gaaaactcag ttggttaacc caactgagtt ttgaagtttt
     8161 atttagtctt tccaaagttc tttaactttt gcttgtactt ctgcattttc taggaattca
     8221 tcgtaggttt catcgatacg gtcaatgacg ccatttttag ataagacaat gatatggtta
     8281 gccaaagttt gaataaattc gtggtcatgg ctggcaaaga tgattgattc tttaaagttt
     8341 ttcaatccat cattcaagct tgagatagat tccaagtcca agtgatttgt tggatcatca
     8401 agtacaagga catttgattt taagagcatg agttttgaaa gcatgacacg aactttttct
     8461 ccccctgaca agacatttac aggtttgtta acttcatctc cagagaagag catacggccg
     8521 aggaagccac gtaggaaagt attgtcatct tcttctttac ttgcgaattg acgcaaccag
     8581 tcaagaattg attctcctcc tgcaaaatca gctgagttat cttttggtag gtaagattga
     8641 ctagttgtaa ctccccactt gacagttcct tcatagtcaa tatctcccat gattgcacga
     8701 attaatgcag tcgtttgaat atcattttgt ccaataagtg ctgttttatc atctggacgc
     8761 aagatgaaac taatattatc caagatagtt tcaccatcaa tctttacagt tagattttct
     8821 actgtcaaga gatcattacc aatctcacgt tccgctttaa agttgataaa tggatattta
     8881 cgactagatg gcacaatctc ttctagctca atcttatcaa gcattttctt acgtgatgtt
     8941 gcctgccttg acttagaagc attggcagag aaacgagcaa caaattcttg caattgttta
     9001 attttttctt ctgctttagc attacggtct gctagcaatt tagcagcaag ctcagaagat
     9061 tccttccaga agtcgtagtt tccgacatag agtttgattt ttccaaagtc aaggtcggcc
     9121 atgtgagtac aaactttgtt taagaagtga cggtcgtggg atactacgat aactgtgtta
     9181 tcaaagtcaa ttaagaagtc ttctaaccaa gtaatcgatt ggatatccaa accgttagta
     9241 ggctcgtcca agagaagaac atctggttta ccaaaaagtg ctttggcgag gagaaccttt
     9301 actttttcac cgttggccaa ttcgctcatg ttttggtagt gtaattcttc tggaatgttt
     9361 aggttttgaa gtagttgaga ggcttcactc tctgcttccc aacctccaag ttcggcaaac
     9421 tctccttcga gttcggcagc acgaaccccg tcctcgtctg agaaatcttc cttcatgtag
     9481 atagcatctt tctctttcat gatgctataa agtttttcat ttcccatgat aacgacatca
     9541 atggcacgtt catcttcgta gtcaaagtga ttttgacgaa gaacagagag acgttcatct
     9601 ggaccaagag agatgtgacc agtagtaggt tcgatatctc cagctaaaat ttttaaaaag
     9661 gttgattttc cggcaccatt agcaccgatt aatccgtaag tatttccttc tgtaaatttg
     9721 atattgacat catcaaaaag tttgcgatca ctaaaacgta gtgaaacatc agatactgta
     9781 agcaatattt ttctcctata tgtgt
//
LOCUS       DAVKWZ010000067         9794 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000067 DAVKWZ010000000
VERSION     DAVKWZ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9794
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.067"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            125..2053
                     /gene="glgB"
                     /locus_tag="VW854_001815"
     CDS             125..2053
                     /gene="glgB"
                     /locus_tag="VW854_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEV0263006.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAILTELPEKKWKDGLWLARRKRWGFEERPVNIYEVHAGSWKRNSDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVIMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEVIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
     gene            2043..3185
                     /locus_tag="VW854_001816"
     CDS             2043..3185
                     /locus_tag="VW854_001816"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEV0263007.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            3175..4314
                     /gene="glgD"
                     /locus_tag="VW854_001817"
     CDS             3175..4314
                     /gene="glgD"
                     /locus_tag="VW854_001817"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEV0263008.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVRTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            4311..5744
                     /gene="glgA"
                     /locus_tag="VW854_001818"
     CDS             4311..5744
                     /gene="glgA"
                     /locus_tag="VW854_001818"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEV0263009.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGFERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            5803..6174
                     /locus_tag="VW854_001819"
     CDS             5803..6174
                     /locus_tag="VW854_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEV0263010.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHCQPA"
     gene            6119..6343
                     /locus_tag="VW854_001820"
     CDS             6119..6343
                     /locus_tag="VW854_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV0263011.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVGQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGVNNLLMLKSAE"
     gene            6457..7572
                     /locus_tag="VW854_001821"
     CDS             6457..7572
                     /locus_tag="VW854_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEV0263012.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLGYQFYDRDGNVLP
                     ASGQSLLNLASVSTENCYKIPEGVQIHILADVVSPLCGHQGATYTFGNQKGLHPTMFA
                     VVDQAIQDFYEKFSPATLEIKGAGAGGGLAGGLCAFAQASIVSGIDTCLDLINFDKKV
                     SDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAEDLPPLPFENIQAV
                     FSILEKSEPLEDSLKNASLYLEHTAANIRRLLSLSKI"
     gene            complement(7569..8015)
                     /locus_tag="VW854_001822"
     CDS             complement(7569..8015)
                     /locus_tag="VW854_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1694 domain-containing protein"
                     /protein_id="HEV0263013.1"
                     /translation="MTDLSKQLLEQAHGGAKLNPDEQRRYLGTFEERVLGYADIDTAN
                     SPQLEKGFLSILEKLQEKADPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHTS
                     SPFGLIIHSNAPVQVEEKNLRLAFPKLWEGKKEEPAKTSIWKKWFS"
     gene            8179..9483
                     /gene="eno"
                     /locus_tag="VW854_001823"
     CDS             8179..9483
                     /gene="eno"
                     /locus_tag="VW854_001823"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEV0263014.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAIATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            complement(9621..9758)
                     /locus_tag="VW854_001824"
     CDS             complement(9621..9758)
                     /locus_tag="VW854_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEV0263015.1"
                     /translation="MPLKTITDRVSHSDSEVTTSIYTHVTKNMKDEAINVLDKVMKKI
                     F"
BASE COUNT         2924 a         1745 c         2177 g         2948 t
ORIGIN      
        1 acgagtaagt gaacttgttg tagattttca aaataattaa agaagtatag taatttactt
       61 gaatcttctc taaaaaggta gtataataaa aacacagaaa acgcttacag gagaagggga
      121 gtatatggat aatagagaag cattaaaaac ctttatgacg ggtgaaaatt tttatctcca
      181 acattatcta ggagcacata gggaagaact aaatggagag catggctata ccttccgtgt
      241 ttgggcacct aatgctcagg ctgttcactt ggttggtgat tttaccaact ggattgaaaa
      301 tcagattcca atggtaagaa atgattttgg ggtctgggaa gtctttacca atatggctca
      361 agaagggcat atttacaaat atcatgtcac acgtcaaaat ggtcatcaac tgatgaagat
      421 tgaccctttt gctgtcaggt atgaggctcg tccaggaaca ggggcaatct taacagagct
      481 tcctgagaag aaatggaagg atggactttg gctggcacga agaaaacgtt ggggctttga
      541 agagcgtcct gtcaatattt atgaagttca cgctggatca tggaaaagaa attctgatgg
      601 cagtccttat agttttgccc agctcaagga tgaactcatt ccttatctcg ttgaaatgaa
      661 ctatactcat attgagttta tgcccttgat gtcccatcct ttgggcttga gttgggggta
      721 tcagcttatg ggttacttcg ctttagagca tgcttatggc cgaccagagg agtttcaaga
      781 ttttgtcgag gagtgtcata cccataatat tggggttatt gtggactggg taccaggtca
      841 ctttaccatc aacgatgatg ccttagccta ttatgatggg acaccgactt ttgaatacca
      901 agaccataat aaggctcata accatggttg gggtgccctt aattttgacc ttggaaaaaa
      961 tgaagtccag tccttcttaa tttcttgcat taagcattgg attgatgtct atcatttgga
     1021 tggtattcgt gtggatgctg ttagcaacat gctctatttg gactatgatg atgctccatg
     1081 gacacctaat aaagatggcg gaaatctcaa ctatgaaggt tattatttcc ttcagcgctt
     1141 gaatgaggtt attaagttag aatatccaga tgtgataatg attgcagaag aaagttcgtc
     1201 tgcgaccaag attacgggaa tgaaagagat tggtggtcta ggatttgact acaaatggaa
     1261 catgggctgg atgaatgata tcctccgttt ctacgaagaa gatccgatct atcgtaaata
     1321 tgactttaac ctggtgactt tcagctttat gtatgttttc aaggagaatt atctcttgcc
     1381 attctcgcac gatgaagtgg ttcatggcaa gaagagtatg atgcataaga tgtggggaga
     1441 tcgttacaat caattcgcag gcttgcgcaa tctctatacg taccaaattt gtcaccctgg
     1501 taagaaattg ctcttcatgg gtagcgaata cggtcaattc ctagaatgga aatctgaaga
     1561 acagttggaa tggtctaacc tagaagaccc aatgaatgct aagatgaagt atttcacttc
     1621 tcagctaaac cagttttaca aagatcatcg ctgtctgtgg gaaatcgata ccagctatga
     1681 tggtattgaa gtcattgatg cggataatcg agaccagagt gttctttcct ttattcgtaa
     1741 gggtaaaaag ggagaaatgt tagtctgtat ctttaacatg gtacctgttg agcggaaaga
     1801 ttttacaatc ggactacccg ttgcaggaat ttacgaagaa gtatggaata ctgagttgga
     1861 agagtgggga ggcgtttgga aagaacataa tcaaactgtt caaacgcaag aaggactatg
     1921 gaaagattat gagcagacct taacctttac cctaccggct atgggagcaa gtgtatggaa
     1981 aatcaaacgt cgcttgaaat ctactaaaac cgtcacaaat aaaaaccaaa aaggagtaga
     2041 aaatgaaaaa tgaaatgtta gctttgattc ttgctggtgg gcaaggaact cgtctcggta
     2101 aactcactca aagcatcgca aaaccagctg tgcaatttgg tgggcgctac cgtatcattg
     2161 actttgccct atcaaactgt gccaactcag ggattcataa tgttggggtc gttacacagt
     2221 atcaaccact tgctctcaac aaccatattg ggaatggttc aagctgggga ctagacggta
     2281 ttaattcagg tgtctctatt cttcaacctt attctgcaag tgaaggaaat cgttggtttg
     2341 aggggactag tcacgctatt taccaaaata tcgactatat cgacagtgtc aatcctgagt
     2401 atgtcttgat tttgtctggg gatcacatct acaaaatgga ctatgatgat atgctccagt
     2461 ctcataagga taataatgcc agcttgacag tagcagtttt agacgtccct cttaaagaag
     2521 caagccgttt tggtatcatg aacacagatg ctaacaatcg tattgttgaa tttgaagaaa
     2581 aaccagctca acctaaatct acaaaagctt ctatgggaat ctacattttt gattggcaac
     2641 gccttcgtaa tatgttagtc gctgctgaaa agagcaaggt tggcatgtca gactttggta
     2701 aaaatgtcat tccaaattac cttgagtcag gtgaaagtgt ttatgcttac gaatttagtg
     2761 gttattggaa agatgttggt actattgagt cactttggga agcgaacatg gagtatattt
     2821 ctccagaaaa tgccttggat agtcgtaacc gtcaatggaa gatttactca agaaacttga
     2881 tttcaccacc aaacttcctc ggggcaaatg ctcatgtgga agactcatta gttgtagacg
     2941 gatgtttcgt tgatggaact gttaaacatt ctatcctttc aacaggcgcg caagttcgcg
     3001 aaggagcgga agtccttgat tcagttatca tgagtggagc tatcattggt caaggagcta
     3061 agattaaacg tgccattatt ggtgaaggtg cgattatttc tgacggtgtc gaaattgatg
     3121 gaacagatga agtacaagtt gtaggatata atgaagtagt gggggtagca acagatgaag
     3181 attgataaat attctgccat tttaggaaat acagttggtt ttcacaatat gtcgacattg
     3241 acggaccacc gtccagtagc aagtttgcca tttggtggga aatatcgttt gattgacttc
     3301 ccactttcaa gccttgctaa tgcaggtgtt cgtagtgtct ttggtatttt ccagcaggat
     3361 aatatcagct cagtatttga ccatattcgt tcaggacgcg agtggggctt atcaaccctt
     3421 cttagccatt actatctagg aatttacaat acccgtgtag aaagtagtac agttggaaaa
     3481 gaatactacc aacagcttct tacttatttg aaacgttctg gctcaaacca aacggttgcc
     3541 ctcaactgcg atgttcttat taacattgat ttgaaccaag ttttccacct acatagtaca
     3601 acaaaagagc ctatcactgt agtttataaa aaactagcta agaaagatat ttcagaagta
     3661 aatgcaatct tggatgtaga tgaaacagac catgttcttt ctcataaact ctttgatagc
     3721 aagtcaacag ctgaaacgtt caatatgtct acagatatct ttgtcgttga tacaccttgg
     3781 ttgattgaac acttggaaga agaagctaaa aaagaacatc cagagaaatt gcgctatgtt
     3841 ttacgggatt tggctgtaaa agagggagct ttcgcctacg agtacacggg ctatctagcc
     3901 aatattcact ctgttaaatc ttattatcaa gcgaatatcg atatgcttga atcacaaaaa
     3961 ttctattctc ttttctcacc aaaccaaaag atttatacaa aggtcaaaaa cgaagagcca
     4021 acttactatg ctaatacatc taaggtaaga acttctcagt ttgcctctgg tagtattatt
     4081 gaaggtcaag tagctaattc tgttctatca cgtaatatcc atgtccataa ggatagcttg
     4141 gttaaagata gcctgctctt ccctcgtgtt gttattggag aaggggctca ggtcgaatat
     4201 gctatcttgg acaaaggggt tgaagttgag cctggtgttg tgattcgagg aactgcagaa
     4261 catccagttg tggttaagaa aggtgctaaa gtaacagagg atattcattc atgaaaattt
     4321 tatttgtagc agcagagggt gcaccctttt caaaaacagg tggtttggga gacgtcattg
     4381 gcgctcttcc aaaatcactg gtaaaagctg ggcacgaagt tgcagtgatt ttaccctact
     4441 atgatatggt agaggctaaa tttggaaatc agattgaaga tgtgcttcat tttgaggtga
     4501 gcgttggttg gcgcagacag tattgtggaa ttaagaaaac agtattaaat ggtgtaacct
     4561 tctactttat tgacaatcaa tattatttct tccgtggtca tgtttacggt gattttgatg
     4621 acggagaacg ctttgccttt ttccaactgg ctgccattga ggctatggaa aggattgact
     4681 ttattcctga tcttctccat gttcatgact accatacagc tatgattcct ttcttgttga
     4741 aggaaaaata ccgttggatt caagcctatg aggacattga aacagtttta accattcata
     4801 atttagaatt ccaaggacaa ttttcagaag gaatgttggg tgatttgttt ggagttggct
     4861 ttgaacgtta cgctgatggc acccttcgat ggaacaactg tctgaactgg atgaaggcag
     4921 gtattctcta tgcgaaccgt gtttcaaccg tttcacctag ctatgctcat gaaattatga
     4981 ctagtcagtt tggatgtaat ttggatcaga ttcttaaaat ggagtctggt aaagtatctg
     5041 gtatcgtgaa tgggattgat gctgatcttt ataatcctca gacggatgct cttttagact
     5101 atcatttcaa tcaggaagat ttgtctggga aagccaaaaa taaggcaaaa ttgcaagaaa
     5161 gagttggctt gcctgttaga gcagacgttc cactggtggg aattgtttct cgtttgacac
     5221 gtcaaaaagg ttttgatgtg gtggtcgaaa gtcttcacca tatcttgcaa gaagatgttc
     5281 agattgttct tttgggaact ggcgatccag cctttgaagg agctttctca tggtttgctc
     5341 agatttaccc agacaagcta tcaacaaata tcacttttga tgtcaaactt gctcaggaaa
     5401 tctacgctgc ttgtgacctc ttcctcatgc caagtcgttt tgaaccgtgt ggcttgtctc
     5461 aaatgatggc tatgcgttat ggaaccttgc cattggtcca tgaagttgga ggcttgcgag
     5521 atacagttcg cgctttcaat ccaatcgaag gaagcggtac tggctttagc tttgacaatc
     5581 tatctcctta ttggttaaat tggactttcc aaacagcatt ggacttgtat agaaaccatc
     5641 cagacatttg gagaaaccta caaaaacaag ctatggagag tgacttctca tgggatacag
     5701 cctgcaagtc ataccttgac ttgtaccata gtttagttaa ttaatagata aaatcgtatg
     5761 atgacttcat acgattttta ggctgtttag agaggagaac tgatgtctca agtaaaaggc
     5821 ttgtgtgttt tggatgtaga tggaacctta atcctagaag aagtgattga tcttttaggg
     5881 agagaggcag gtcatgaggc ggaaatttcg cagattacaa gtcgagcaat gcgaggagag
     5941 ttggtctttg aaagcagttt aagaaaaaga gtgtccttgt tggaaggtct tcctattttg
     6001 gtctttgata acgtcttcaa ctcaattcat ctatcgctaa atgtcccaga gttcatctct
     6061 attctccaaa agaatggcat cctagttggt ctggtgtctg gtggatttac accaatagtt
     6121 ggagagatta gcaaaatccc ttggtattgc ctatttcact gccaaccagc ttgaagtcaa
     6181 agaaggtctt ttaacaggaa aattagttgg acaaattata agtccccagg tcaaaaaaga
     6241 aactctggaa aaatggagaa agaaactaaa actttctaaa gaaagaacgg tggcaatcgg
     6301 tgatggggtc aataatctat taatgttgaa gtcggcggag tgaggaatcg ccttttgttc
     6361 caaagaaatg ctcaaaaaag aaataccaca tcatgttgac aagagggatt ttttagaagt
     6421 tcttcctttg attgactgtt tagaatgagg gaaaatatga agattgtaat tgcaccggat
     6481 tcgtttaagg aaagcttgac agctcaacag gtagctgaag caataaaaag aggcttccaa
     6541 caatcgatag cagatgtaga atgtctcctc tgccctgttg gtgatggggg agaaggtact
     6601 gtagatgcta tccgacattc tcttgaccta aaagaaaaat ggatccaagt gacagaccct
     6661 tttggacaaa aagaagccat gcgctatttt caaaaagggg aactggcact atttgaagta
     6721 gctgacttgg ttggtcttgg aaaaattccg ctagagaaac gaaatccact tcaaatccaa
     6781 acttgtggta ttggagagtt gattctccat ctcatttcta aagggattaa agatatttat
     6841 atcggtgttg gtggcacggc cagtaatgat ggaggactgg ggattgctgc tggtttaggt
     6901 tatcaatttt atgataggga tggaaatgtc ttgcctgctt ccggtcaatc cttattaaac
     6961 ttagcttctg tttcaacaga aaattgctat aaaattcctg aaggtgttca aattcatatt
     7021 ttagcagatg tcgtgagtcc cttatgtggt catcaaggtg cgacttacac ttttggcaat
     7081 caaaaaggtc tacatccgac tatgtttgca gtcgtagatc aggcgatcca agatttttat
     7141 gaaaaattct cacctgcaac attagaaatt aaaggagcag gagcaggtgg aggccttgct
     7201 ggtggtttgt gtgcctttgc tcaggcaagt atcgtgtctg gaattgatac ctgcttggac
     7261 ttaatcaact ttgataagaa agtttcagat gctgacttgg ttgttgttgg agaaggaaga
     7321 cttgatagtc aaagctttgc tgggaaagcg cctataggtg ttgcaaaaag aacccctgtt
     7381 ggagttcctg ttattgctat ttgcggtagt cttgctgaag atttacctcc cctaccattt
     7441 gaaaatatcc aagctgtctt ttctattttg gagaaaagtg aacctttaga agatagtttg
     7501 aaaaatgcca gtctctattt ggagcacacg gctgccaata ttaggcgttt attaagtctg
     7561 agtaagattt agctaaacca tttcttccag atggatgttt tggcaggttc ttccttttta
     7621 ccttcccaaa gttttggaaa agcaagtcga aggttctttt cttctacttg tactggtgca
     7681 ttgctatgga taatcagacc gaaaggagaa gaagtatgtt cctcagatac tatggtagct
     7741 tgactatcgg tttctttggc ttccttcaag tagaaaactt gcttgtcaaa ttcgatagtt
     7801 ggtgaaatct tcacaaatag tggatctgct ttttcctgaa gtttttctaa aatagataaa
     7861 aagccttttt ctaactgagg actatttgct gtgtcaatat ctgcatatcc aagaactctt
     7921 tcctcaaaag taccaagata gcgtctttgc tcatccggat ttaattttgc tccaccatga
     7981 gcttgttcaa gtaattgttt tgataaatct gtcatgaaaa cagtatatca taaaagttag
     8041 aataaaacag acaaaagaaa gcgattactt tataaagtta aggaaaagtt gcacaaagat
     8101 aacgtttttt cttgaaaaag gaggggtttt aaggtattat agaggtgtaa aaaatttaac
     8161 tcataaggag agtaaaaaat gtcaattatt actgatgttt acgctcgcga agtcctagac
     8221 tcacgcggta acccaacact tgaagtagaa gtttacactg aatcaggtgc tttcggacgt
     8281 ggtatggttc catcaggagc ttctactggt gaacacgaag cagttgaact tcgcgacggt
     8341 gacaaatctc gttacggtgg tcttggtaca caaaaagctg ttgacaacgt aaacaacatc
     8401 attgctgaag ctatcattgg ctacgatgta cgtgatcaac aagctatcga ccgtgctatg
     8461 atcgctcttg acggtactcc taacaaaggt aaattgggtg caaacgcaat ccttggtgtg
     8521 tctatcgctg tagctcgtgc tgctgctgac taccttgaaa tcccacttta cagctacctt
     8581 ggtggattca acactaaagt tcttccaact ccaatgatga acatcatcaa cggtggttct
     8641 cactctgacg ctccaatcgc tttccaagag ttcatgatct tgccagttgg tgcgccaaca
     8701 tttaaagaag cccttcgtta cggtgctgaa atcttccacg ctcttaagaa aatccttaaa
     8761 tcacgtggtt tggaaactgc cgtaggtgac gaaggtgggt tcgctcctcg tttcgaagga
     8821 actgaagatg gtgttgaaac tatccttgct gcgattgaag ctgctggata tgtaccaggt
     8881 aaagacgtat ttatcggatt tgactgtgct tcatcagaat tctacgataa agaacgtaaa
     8941 gtttacgact acactaaatt tgaaggtgaa ggtgctgctg ttcgtacatc tgcagaacaa
     9001 atcgactacc ttgaagaatt ggttaacaaa tacccaatca tcactattga agatggtatg
     9061 gatgaaaacg actgggatgg ttggaaagct cttactgaac gtcttggtaa gaaagtacaa
     9121 cttgttggtg acgacttctt cgtaacaaac actgactacc ttgcacgtgg tatccaagaa
     9181 ggtgctgcta actcaatcct tatcaaagtt aaccaaatcg gtactcttac tgaaactttt
     9241 gaagctatcg aaatggctaa agaagctggt tacactgctg ttgtatcaca ccgttcaggt
     9301 gaaactgaag attcaacaat cgctgatatt gcaattgcaa ctaacgcagg acaaatcaag
     9361 actggttcac tttcacgtac agaccgcatc gctaaataca accaattgct tcgtatcgaa
     9421 gaccaacttg gtgaagtagc tgaatatcgt ggattgaaat cattctacaa ccttaaaaaa
     9481 taaaatagtt cagtgaacta ttttatcccg aaccatgaaa ttcaaaagtt cggccgttga
     9541 tttaacaacg tttctagccc ctcggatttt atccgaaggg ctatttttgt atttagagga
     9601 caaaaaaggg acaaaacttt ttaaaaaatc tttttcataa ctttatccag tacattgatt
     9661 gcttcatctt tcatgttctt tgtgacgtgg gtgtagatgg aagtagtgac ttcagagtcg
     9721 gaatgactaa ccctgtccgt gattgttttt aaaggaattt tattggctct ttgtcaactg
     9781 tagtgggttg aagt
//
LOCUS       DAVKWZ010000068         9656 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000068 DAVKWZ010000000
VERSION     DAVKWZ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9656
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.068"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            <1..280
                     /locus_tag="VW854_001825"
     CDS             <1..280
                     /locus_tag="VW854_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV0263016.1"
                     /translation="GSWYYLNSNGAMVTGWLQNNGSWYYLNANGSMATDWVKDGDTWY
                     YLEASGAMKASQWFKVSDKWYYVNGSGALAVNTTVDSYRVNANGEWVN"
     gene            complement(510..1490)
                     /gene="dusB"
                     /locus_tag="VW854_001826"
     CDS             complement(510..1490)
                     /gene="dusB"
                     /locus_tag="VW854_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEV0263017.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            complement(1477..2349)
                     /gene="hslO"
                     /locus_tag="VW854_001827"
     CDS             complement(1477..2349)
                     /gene="hslO"
                     /locus_tag="VW854_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEV0263018.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGAGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            2514..3941
                     /locus_tag="VW854_001828"
     CDS             2514..3941
                     /locus_tag="VW854_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV0263019.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELAIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSVGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPTFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QPPVYYLKNDLDMEDLAGIRQLLFT"
     gene            4100..5608
                     /gene="glpK"
                     /locus_tag="VW854_001829"
     CDS             4100..5608
                     /gene="glpK"
                     /locus_tag="VW854_001829"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEV0263020.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            5650..7476
                     /gene="glpO"
                     /locus_tag="VW854_001830"
     CDS             5650..7476
                     /gene="glpO"
                     /locus_tag="VW854_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEV0263021.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKKEVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKYLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            7546..8250
                     /locus_tag="VW854_001831"
     CDS             7546..8250
                     /locus_tag="VW854_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV0263022.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTIGVALKGGLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            8441..8644
                     /locus_tag="VW854_001832"
     CDS             8441..8644
                     /locus_tag="VW854_001832"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263023.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEGKKQELLNRLNK"
     gene            complement(8731..9339)
                     /locus_tag="VW854_001833"
     CDS             complement(8731..9339)
                     /locus_tag="VW854_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263024.1"
                     /translation="MSSISPSLVVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNI
                     LSDEVLEVVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVN
                     TIHGNKQANTFANFMTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSP
                     YPYYITSTYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
BASE COUNT         2778 a         1924 c         2212 g         2742 t
ORIGIN      
        1 tggttcatgg tactacctaa acagcaatgg tgctatggta acaggatggc tccaaaacaa
       61 tggctcatgg tactacctaa acgctaacgg ttcaatggca acagattggg tgaaagatgg
      121 agatacctgg tactatcttg aagcatcagg tgctatgaaa gcaagccaat ggttcaaagt
      181 atcagataaa tggtactatg tcaatggctc aggtgccctt gcagtcaaca caactgtaga
      241 tagctataga gtcaatgcca atggtgaatg ggtaaactaa acttaatata actagttaat
      301 actgacttcc tgtaagaact ctttaaagta ttccctacaa ataccatatc ctttcagtag
      361 ataatatacc cttgtaggaa gtttagatta aaaaataact ctgtaatctc tagccggatt
      421 tatagcgcta gagactacgg agtttttttg atgaggaaag aatggcggca ttcaagagac
      481 tctttaagag agttacgggt tttaaactat taagccttct ccaattgcaa gagggcttca
      541 atctctgcta gggtgctagc ttgcgaaatg gctccacgga gtttggcagc gccagatgtt
      601 ccacggaggt agtgaggagc gaggccgcgg aattcacgaa ctgcgacgtt ttctcctttg
      661 aggttaatca atcgtttcaa gtgttcgtag gcgatcttca tcttgtcttc aaaggtcaaa
      721 tcaggtagga tttctcctgt ttcaaagtaa tggttgattt ggttgaagag gtaaggattt
      781 cccatggcag ctcggccaat catgactgcg tcagcaccaa cttcttcgat gcgttgcttg
      841 gcttcttgga cagtacggat atcaccgttg gcgatgaatg gaatcttggt tagagcttgg
      901 gcaaccttgt aaagggtctc aaggtctgcg tggccagtat acatttgttc acgggtacgg
      961 ccatgcatgg cgagggcaga aacacctgca gcttcagcag cgagggcatt ttctactgcc
     1021 agagatgggt ccgcccagcc ggtacgcatt ttgacagtaa gtgggatatc aaggacagac
     1081 tggaccttgt tgatgataga gtagatcttg tcaggatcct tgagccacat agcaccagct
     1141 tcgttcttca cgattttgtt gacagggcag cccatgttga tatcgacgat atcggtcttg
     1201 gtgttttctt ggatgaattc tgctgcgcgt gctaggctgt cttcatcgct accaaaaagt
     1261 tggatagaga cagggttttc gccctcatcg atatgaagca tgtgcagggt tttttcgttg
     1321 ttgtattgga ttcccttgtc agagaccatt tccattacaa cgagtccagc tccgagctct
     1381 tttgcgatgg tacgaaaggc tgagttggtc acgccagcca taggcgctaa aacggtacga
     1441 ttgggaatct caacattgcc aatcataaaa ggtgtattaa gatttgtcac gaatgagttc
     1501 ctccaggtcc ttttcatcaa agttgtaagt agtttggcag aattgacaag tgatttctgc
     1561 cccgtggtct tcctctttca tttcctgtaa gtctgagctt ggaaggctgg caagagcgtt
     1621 cataaagcgt tcatggctac agtcacattg gaaacggatt tcttcttcag aaagacgctt
     1681 gtaggcttcg tccccgtaga tagccttgag gagggcttcg atatggtcgt cgctttcgag
     1741 aagagtagag atagctggca tttcttggat gcgtttttca aagcgagcaa tctcttcttt
     1801 cttggctcct ggcaagactt gaactaggaa accacctgca accttgacct tgtcttcctc
     1861 gtccaaaagg acattgaggc cgaccgctga aggcgtttgt tggctttcag taaggtaaaa
     1921 ggcaaggtct tcaccgattt ctccagagat gaggggagtt atagagttgt aaggatttcc
     1981 agcaccgtag tctgtgataa cgaggaattg accatttcca acaaaaggtc cgactaggac
     2041 ttcaccagtc gcagtctttt tgatgtcaac accaggattt tgaacatagc ctttgacgtt
     2101 ccccttggta tcagcgacgg tgataatagc acctagagag ctagagccca acaccttaac
     2161 tgtaagtttg gtatttcctt tttcattggc tgcgagaatc tggctggcga taagagttcg
     2221 accaagcgct acagttgagc tagcttgggt ttgatgtttt tcttgagcag tgcggacggt
     2281 ttccgtgcta tcaaggacaa aagcacgaaa ggctccgctt tctgatatag ttttaataat
     2341 tttatccata gctactattt tagcataaaa atgcccaaag ggggagccgt gtgtttactg
     2401 attttcagga taatggacca ggaaatcagc atgaaaataa aaagagaaac agattatttt
     2461 agcatttgta agatttatgc tatgcttaag gtagaaaatg aaaggggtaa caaatgtatt
     2521 taggagattt gatggagaaa gccgagtgtg gtcaattttc aatcctttcc tttctattac
     2581 aagagtctca gacgaccgtc aaggctgtaa tggaagaaac aggattttca aaagcaaccc
     2641 taaccaaata tgtcaccctg ctcaatgaca aggctttgga tagtggctta gagctggcta
     2701 ttcactcaga agatgaaaat ctgcgtctgt ctatcggtgc agctaccaag gggagagata
     2761 ttcggagctt gtttttggag agtgctgtta aataccagat tttggtttat cttctctacc
     2821 accaacagtt tttagcccat cagctggctc aagaattggt gattagcgag gctacgcttg
     2881 gtcgtcactt ggctggttta aatcagattt tgtcagaatt tgatttatcc atccaaaatg
     2941 gacgttggcg aggtccagag catcagattc gctatttcta tttctgtctt ttccgaaagg
     3001 tctggtcgag tcaggaatgg gaaggtcaca tgcagaaacc agagagaaaa caggagattg
     3061 ccaatttaga ggaaatctgc ggtgcaagtt tgtctgtggg gcagaaattg gacttggttc
     3121 tctgggctca catcagtcaa caacgtcttc gggtcaatgc ttgtcagttt caagtcatag
     3181 aagagaaaat gcgagggtat tttgacaata ttttctacct tcgtttgctg agaaaggttc
     3241 cgtccttttt tgctgggcaa catattccac taggagttga ggatggtgag atgatgatat
     3301 tcttctcttt tctcctatct catcgcattc ttcctcttca tactatggag tatattcttg
     3361 gttttggagg gcagttggca gatttactga cgcaattgat tcaagaaatg aagaaggagg
     3421 aactattggg ggattataca gaggaccatg tcacctatga actcagtcag ctttgtgctc
     3481 aagtctatct ctataagggc tatattttac aggatcgcta caagtaccag ttagagaatc
     3541 gtcatccata tttactgatg gaacatgatt ttaaagagac agcagaggag atttttcatg
     3601 ctctacctac ttttcaacag gggacagatt tagataagaa gattctctgg gaatggctcc
     3661 agttaatcga atatatggct gaaaacggtg gccagcatat gcggattggt ctggatttga
     3721 catctggttt tcttgtcttt tcaaggatgg cagccatttt gaaacggtat ttggaataca
     3781 atcgttttat taccattgaa gcttatgacc ctagtcggca ttatgatttg ctggttacca
     3841 ataacccgat tcataagaag gaacagcccc cagtttatta tttaaaaaat gacttggata
     3901 tggaagattt ggcagggatt cgtcagttat tattcactta aaaggcttgg ttaatccagg
     3961 tcttttttgt gaaattcaca caatctcctc acattttttt aaaaattaaa aaaagttgat
     4021 aaacaagaaa gcgctttatt ttgtatacta gtaagtgtaa agaggaaaca cctcaagatc
     4081 tttatcagga ggacagtaca tgtcacaaga aaaatacatc atggccattg accagggaac
     4141 tacaagttct cgtgccatca ttttcaacaa aaaaggggaa aaggttagct cgagtcaaaa
     4201 agagtttacc cagattttcc ctcaggcagg ttgggttgag cacaatgcca atgaaatttg
     4261 gaactctgtt cagtcagtta ttgcgggtgc tttcatcgaa agtggtgtca agccaaatca
     4321 aatcgaggca atcgggatta ccaaccaacg tgaaacaacg gttgtctggg ataagaaaac
     4381 aggacttcct atctacaatg ctatcgtttg gcagtcacgc cagacagcac ctttggctga
     4441 gcaactaaaa agccaaggtt atgtggaaaa attccatgaa aagactggtt tgattattga
     4501 tgcttacttc tctgctacca aggttcgttg gattttggat catgtagaag gtgctcaaga
     4561 gcgagcagaa aaaggggaat tgctctttgg tactatcgat acttggttgg tttggaaatt
     4621 gactgacggt gcggctcacg tgactgacta ctcaaatgca gctcgtacca tgctttataa
     4681 cattaaagaa ctcaaatggg atgatgagat tttggaaatc cttaacattc cgaaggctat
     4741 acttccagaa gttcgttcta actccgaaat ctacggcaag acagctccat tccatttcta
     4801 cggtggagag gtgccaatct caggtatggc tggggaccaa caagcagccc tctttggaca
     4861 gttggctttt gagccaggta tggttaagaa tacttatgga acaggctctt tcatcatcat
     4921 gaatactggg gaagagatgc agttgtctga aaacaacctc ttgacaacca ttggttacgg
     4981 aatcaacggt aaggtttatt atgccttgga aggttctatc ttcatcgcag gaagtgctat
     5041 tcagtggctt cgtgacggtc ttcgcatggt tgaaaattca ccagaatctg aaaaatacgc
     5101 tcgtgattct cacaacaacg atgaagttta tgtcgttcca gcctttacag gtctaggcgc
     5161 tccatactgg aaccaaaatg ctcgtggttc cgtctttggt ttgactcgtg gaacaagcaa
     5221 agaagacttt atcaaggcga ctttgcaatc tattgcttat caagtgcgtg atatcatcga
     5281 caccatgcaa gtggatactc agaccgccat tcaagtactg aaggtggatg gtggtgcagc
     5341 catgaacaac ttcctcatgc agttccaggc ggatatttta ggcattgaca ttgcacgtgc
     5401 taaaaacttg gaaacaacag ctctaggagc ggccttccta gcaggtttgt cagtagggta
     5461 ctggaaagac ttggacgagt tgaaactctt gaacgagaca ggagaactct ttgagccatc
     5521 tatgaacgaa tctcgcaagg aacaactcta caagggctgg aagaaggctg tgaaagcaac
     5581 tcaagtcttt gcggaagtag acgactaata ctggcagaat aaagcgattt atttagaaag
     5641 tgtgtaaata tggaattttc aaaaaaaaca cgtgaattgt caattaaaaa aatgcaggaa
     5701 cgcaccctgg acctcttgat tatcggtgga ggaatcacag gagctggtgt agccttgcag
     5761 gcggcagcta gcggtcttga gactggtttg attgaaatgc aagactttgc agaaggaaca
     5821 tctagtcgtt caacaaaatt ggttcacgga ggtcttcgct acctcaaaca attcgacgta
     5881 gaagtggtct cagatacggt ttctgaacgt gcagtggttc aacaaatcgc tccacacatt
     5941 ccaaaaccag atccaatgct cttaccagtt tacgatgaag atggagcaac ctttagcctc
     6001 ttccgtctta aagtagccat ggacttgtac gacctcttgg caggtgttag caacacacca
     6061 actgcgaaca aggttttgag caagaaagaa gtcttggaac gccagccaaa cttgaagaag
     6121 gaaggcttgg taggaggtgg agtgtatctt gacttccgta acaacgatgc gcgtctcgtg
     6181 attgaaaaca tcaaacgtgc caaccaagac ggtgccctca ttgccaacca cgtgaaggca
     6241 gaaggcttcc tctttgacga aagtggcaag attacaggtg ttgtagctcg tgatctcttg
     6301 acagatcaag tatttgaaat caaggcccgt ctggttatta atacaacagg tccttggagt
     6361 gataaagtac gtaatttgtc taataaggga acgcaattct cacaaatgcg cccaactaag
     6421 ggagttcact tggtagtgga ttcaagtaaa atcaaggttt cacagccagt ttacttcgac
     6481 acaggtttgg gtgacggtcg tatggtcttt gttctcccac gtgaaaacaa gacttacttt
     6541 ggtacaactg atacagacta cacaggtgat ttggagcatc caaaagtaac tcaagaagat
     6601 gtagattatc tacttggcat tgtcaacaac cgcttcccag aatccaacat caccattgat
     6661 gatatcgaaa gcagctgggc aggtcttcgt ccattgattg cagggaacag tgcctctgac
     6721 tataatggtg gaaataacgg taccatcagt gatgaaagct ttgacaactt gattgcgact
     6781 gttgaatctt atctctccaa agaaaaaaca cgtgaagatg ttgagtctgc tgtcagcaag
     6841 cttgaaagta gcacctctga gaaatatttg gatccatctg cagtttctcg tgggtctagc
     6901 ttggaccgtg atgacaatgg tcttttgact cttgctggcg gtaaaatcac agactaccgt
     6961 aagatggctg aaggagctat ggagcgcgtg gttgacatcc tcaaagcaga atttgaccgt
     7021 agctttaaat tgatcaattc taaaacttac cctgtttcag gtggagaatt gaacccagca
     7081 aatgtggatt cagaaatcga agcctttgcg caacttggag tatcacgtgg tttggatagc
     7141 aaggaagctc actatctggc aaatctttac ggttcaaatg caccgaaagt ctttgcactt
     7201 gctcacagct tggaacaagc gccaggactc agcttggcag atactttgtc ccttcactat
     7261 gcaatgcgca acgagttggc tcttagccca gttgacttcc ttcttcgtcg taccaaccat
     7321 atgctcttta tgcgtgatag cttggatagc atcgttgagc cagttttgga tgaaatggga
     7381 cgattctatg actggacaga agaagaaaaa gcaacttacc gtgctgatgt cgaagcagct
     7441 ctcgctaaca acgatttagc agaattaaaa aattaagaaa aaataaaaga ggtggagggc
     7501 agcattcctt gtcgcccgtc ccttcttttt aatggagaca gaaagatgat gaatgaatta
     7561 tttggagaat ttctagggac tttaatcctg attcttctag gaaatggtgt tgttgcaggt
     7621 gtggttcttc ctaaaaccaa gagcaatagc tcaggttgga ttgtgattac tatgggttgg
     7681 gggattgcag ttgcggttgc agtctttgta tctggcaagc tcagtccagc tcatttaaac
     7741 ccagctgtga ccatcggtgt ggccttaaaa ggtggtttgc cttgggcttc cgttttgcct
     7801 tatatcttag cccagttcgc aggggccatg ctgggtcaga ttttggtttg gttgcaattc
     7861 aaacctcact atgaggcaga agaaaatgca ggcaatatcc tggcaacctt cagtactgga
     7921 ccagccatca aggatactgt atcaaacttg attagcgaaa tccttggaac ctttgttttg
     7981 gtgttgacaa tctttgcttt gggtctttac gattttcagg caggtatcgg aacctttgca
     8041 gtgggaactt tgattgtcgg tatcggtcta tcactaggtg ggacaacagg ttatgccttg
     8101 aacccagctc gtgaccttgg acctcgtatc atgcacagca tcttgccaat tccaaacaag
     8161 ggagacggag actggtctta cgcttggatt cctgttgtag gccctgttat cggagcagcc
     8221 ttggccgtgc ttgtattctc acttttctaa tctagaaaac aattatgttg atagagcttg
     8281 ggcaagagcc caatttcagc aaaaaatgaa gtaaatcttc tcataataaa acgcatcata
     8341 tcaagcacga aaattccacg aggtcaacta cagtcagaaa gctgaacaac aagccaaaac
     8401 gcccaaaaaa ggcggcaaaa agcaagcacc tgcaagcaac gtgccgaaat ggtcaaatcc
     8461 tgattatgtc aacgaattag acccaaaaat cgttgatatg ctagtagaat ttcacaagtc
     8521 acaaggcact ttggaaactc ccgaggcgca agcagaaatc gcccaaaaac gtgaagaaat
     8581 cgagcaaagg agagctgagc ttgagggtaa aaaacaagag cttttgaacc gcttgaacaa
     8641 atagagtttc gcaagtatta tgcttacaaa ttacttgagc aattaactaa aatataaacc
     8701 ctgcctttat atctaggcag ggtttatatt ttagaaattc acgtaggttg ttacggtttt
     8761 tacataccca gtatagtttg agtttctata gtattcagtg ataaacttcc attttctttg
     8821 agcaacatgg atataagtac ttgttatgta gtatggatat gggctttgtg aatccaagta
     8881 agactgataa gcttgtatac caaaatatgc tccaccaatt attgcacccc atggaccccc
     8941 caataaagca cctatcctac caatcatata actgattcca gcaccagtca tgaagttagc
     9001 gaatgtgtta gcttgtttat tcccatgtat tgtgttgacg taattccaaa cattaggatc
     9061 gtatgatcta aaagatatat ttaggtcgat ttcattcttt tgataagcca tataaaatgc
     9121 cccattgata tagacgccgt cagcacgtcg ttcaatagtg tctacacttc catctggatt
     9181 gacaacctca agaacttcat cgcttaaaat atttacttgc gtatctccga accgcactga
     9241 tgagccattc tcaaactgag cctcaccaga tacaacttta gagtttgccg ataagctatc
     9301 atcagcaaaa acaacaagcg acggggaaat gctagacata cagaaaacag acataactag
     9361 caaacacatg catttaaaca tcttagacat aacggaaact cctttgtatt tttgattttt
     9421 ttcaactttt attatacaat aaaaccaaat aaaaagaaag cggtaacaat atgcttaatg
     9481 cgaaaatttt ttatatattt ttatgtttga tcgttatcga aactacaggc ttgttgttgt
     9541 tgaaaagagg tctcgaaatg ggttatttag acacagaagc tattatcctc gcagtttttt
     9601 catttgcttt ttacaaccta tgttcattcg cttgggtctg ctctacaata aaaaat
//
LOCUS       DAVKWZ010000069         9341 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000069 DAVKWZ010000000
VERSION     DAVKWZ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9341
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.069"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            86..1567
                     /locus_tag="VW854_001834"
     CDS             86..1567
                     /locus_tag="VW854_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV0263025.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSP
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            1929..2924
                     /locus_tag="VW854_001835"
     CDS             1929..2924
                     /locus_tag="VW854_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV0263026.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLRKVQR
                     AIKTLDYIPDNYARGMKKNRTETIALIIPSIWHPFFSEFAYHVEKELLKYNYKLFICN
                     ADSDSQNEIEYIEMLKQNKVDGIIGITYSDIDKYILSDLPFVSIDRHFSENVCYVTSA
                     NYKGGQTAAEELVRRDNKHLAFIGSINMYENETILRSKGFREKAKELGVDVYNFEMKE
                     PLINPEDQIRIFLQSHKEIDGIFTINDMMALRVISVMKELGKIAPDDYQIIGFDGLKT
                     SVDQSYLVSTIVQDIGMMAKESVAMILKMIKREEILEKRIVIPTYFWGDKTTKKI"
     gene            3052..4362
                     /locus_tag="VW854_001836"
     CDS             3052..4362
                     /locus_tag="VW854_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEV0263027.1"
                     /translation="MRKGTMLSVVAGLSLAILAGCSGGTNSKQASSSNDIKVWVQFSD
                     ETNEGKAWQKVVDNFNQSGKSKYKVVTEYIPRSGSGGGYEDKVNAAITTNSLPDVITL
                     DGPNTAAYAKSKVITPLDDYLKDNNMDDVLDSIKQQGTYNGKFYAFGFSESNVGVYYN
                     KKMFKEAGIAESELPTLEKPWTWDEFNTIAKKLKDHYNKPAIDFRINSNDEMLPYAYM
                     PLIWSNNGSVVNEDGTKAEGYFNSKESVEAVQFIQNLVKEGYTTVSPVEKGFETGEYP
                     MLLSGSWTIADMNTNYKDIDFGILPYPVSSKTKKLVSPSGSWQLAVTTKSDKKEAASE
                     FVKFATNTESSEIISLGNSVLPIRKSTIENIKDKVSEPMRFLMEQNSKTAHARPVVVA
                     YPQVSRAFQQAMQDISYYEENPNVQKVLDTRTKEMQTAIDQSLK"
     gene            4458..5360
                     /locus_tag="VW854_001837"
     CDS             4458..5360
                     /locus_tag="VW854_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV0263028.1"
                     /translation="MSKETVVDTKRREKIKDNILGYSFLAPALILLGIFLVIPVGMVI
                     YYAFTDYYLLTPDERKFVGLENFIRLTKDPIFLKSFLNTLKFVVWIIPVQLGAALGMA
                     LIVNKKRKGNMFFKVAFFAPVVMSLVVISILWLYLLNPNSGLLNAILNKVGIASQPFL
                     TSPKQAMYAIVFVSAWQGAGYQMLLFLGGMQNIPQDVYEAAELDGFSKWAQFRYITMP
                     LLKPTALFVLLTTLISAFKLIVQPMVMTQGGPLNSTITMVYYIYQQGFTDRLVGYSSS
                     IALVFTTLIGMISLVQRRVLKEDD"
     gene            5362..6207
                     /locus_tag="VW854_001838"
     CDS             5362..6207
                     /locus_tag="VW854_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV0263029.1"
                     /translation="MKRFKPTTILEYILLTLLAGLFLFPMVWMVVSAMKPEADVYKNL
                     TSWQAFLPSLNPADWFKPYQEVFERFSIFTYLGNSIFYAGTFAIGSIIVNALAGFAFA
                     KVNFSGKKILFGFLLALLIIPVETVLIPQFTIINSLGLVNNRLAVIIPGLASVFNIYL
                     FRNFFIAIPEEILESAKMDGASIVRIFFRIMLPMSKPAVATVGVLSFISSWNDYIWPL
                     MVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTISTIPLILVYVVAQKYIVQGLGGS
                     GTGIK"
     gene            6217..7536
                     /locus_tag="VW854_001839"
     CDS             6217..7536
                     /locus_tag="VW854_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV0263030.1"
                     /translation="MINKTFTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEKTGVRQVQNMAFSDDGIHFEKIEQNPVATGADLPDELIA
                     ADFRDPKLFEKDGRYYSVVATKHKDNVGCIVLLGSDNLVEWQFESIFLKGREHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWGEKRFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDTDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            7931..8344
                     /locus_tag="VW854_001840"
     CDS             7931..8344
                     /locus_tag="VW854_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263031.1"
                     /translation="MKNNERLGIKLSRDSVLGLREVRRLYLGSSDIPVPDGYVIEVAY
                     KQISNEIDIIDWVELNKSKIKISEISESVDIDATSLRTTLTLDTLVYEGMRDIQLKLR
                     ELTKGRVFFSFVVKLVLFASILKKKGLLEKFQEKC"
     gene            8582..8881
                     /locus_tag="VW854_001841"
     CDS             8582..8881
                     /locus_tag="VW854_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263032.1"
                     /translation="MNRNVLEFLKTETAEKISLFIRKINGLEGNVTLLSINSQDLEDI
                     KNAMLSNSNLGLKIARLDVMKKIAYASNRTHYKDGTTIMDDISSGKIHRRPKSYI"
     gene            8935..9156
                     /locus_tag="VW854_001842"
     CDS             8935..9156
                     /locus_tag="VW854_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000916559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263033.1"
                     /translation="MLKNFCQNAFISLYFIVSLLGGFSAQVDLKSFSPYQSMKIALLL
                     GVALILLQLPIMFAKLLEALKTKKGETHE"
BASE COUNT         3133 a         1324 c         1768 g         3116 t
ORIGIN      
        1 agtcgttata tcatgctata cctattcttt gtggtataat tgcaagaggt ttaatccgat
       61 aatttataaa gagagaaaga tattcatgag agatttatta tctaaaaaaa gtcataggca
      121 attagaatta ttagaattat tatttgaaca taaacgttgg tttcatcgtt ctgaactagc
      181 agagttatta aattgtacag aacgtgcagt caaagatgat ctatcccatg ttaaatctgc
      241 ttttcctgac ttgatttttc attcttctac taatggtata cgcattatta ataccgatga
      301 tagtgatatt gaaatggttt accatcattt ctttaaacat tcaactcatt tttcgatttt
      361 agaattcatc ttctttaatg aaggttgtca agctgagagt atttgtaaag aattttatat
      421 cagttcatct tcgctctatc gtattattag ccaaatcaat aaagtgatta aaaggcaatt
      481 tcaatttgaa gtcagtctga cccctgttca aatcattgga aatgagagag acattcgtta
      541 cttttttgca caatattttt cagaaaaata ttatttccta gaatggccat ttgaaaattt
      601 ttcatcagag ccactatctc aattgttaga attggtttat aaggaaacaa gctttccaat
      661 gaatttgtca acgcatagaa tgctaaagtt gctcctagtt acgaacctat atagaataaa
      721 gtttggtcat tttatggaag tagataaaga ttcttttaac gaccaaagtt tggatttttt
      781 aatgcaggca gaaggaatag aaggtgttgc tcagagtttt gaatcagaat acaatatctc
      841 tttagatgaa gaagttgttt gccaattatt tgtgtcttac tttcaaaaaa tgtttttcat
      901 agatgaaagt ctctttatga aatgcgtaaa aaaggatagt tatgttgaaa aatcttacca
      961 tctattgagt gattttattg atcagatttc agtcaagtat cagattgaaa tggagaataa
     1021 ggataatctg atttggcatc tgcataatac cgcacatctg tatcgtcaag agttgtttac
     1081 tgagtttatt ttatttgatc aaaaagggaa tacaatcagg aactttcaaa atatttttcc
     1141 aaaatttgtt tcagatataa aaaaagagct ttctcactat ttagagactc ttgaggtttg
     1201 ttctagttca atgatggtaa atcatctatc ttataccttc atcactcata ccaaacattt
     1261 ggtgattaat ttgctacaaa atcagcctaa actaaaggtt ttggttatga gtaattttga
     1321 tcagtatcat gcaaaattcg ttgcagagac actttcttat tactgtagca ataattttga
     1381 actggaggtt tggaccgaat tagaattatc aaaggaatct ttagaagatt caccttatga
     1441 tattattatt tccaatttta ttattcctcc tattgaaaat aagagactca tctattcaaa
     1501 taatataaac acggtctcac tcatatactt gttaaatgcc atgatgttta ttcgattaga
     1561 tgagtaacca aaaataacaa aaaaacgcta tttcgcgttt gaactgtacc tcaaaagtta
     1621 gacagaaaaa gtctaatttt tagggtgttt ttgttaagaa aagaccttca atcatttaag
     1681 aaaaatgtac atctagattt atagccctct aaaactcttg ctttttttgt agaagatacg
     1741 aaaaaagcca tgaaaccaag gcttttcctg ttttatgtag atgtttacac atgaaaattg
     1801 aagtattttc aaaagctaat tggagtgatt ttattcatga tgggataaat gatctggcca
     1861 aaaatgattg agaaatagtt gcttaaaaag catttgcatg atactatata ttgaaaggag
     1921 gtgataatat ggtagaacaa agaaaatcaa ttaccatgaa agatgttgct ttagaagcag
     1981 gagttagtgt tggaactgtt tcacgtgtaa ttaataaaga aaaaggcatt aaagaagtaa
     2041 ctttaaggaa agttcaaagg gctataaaaa cattggacta tatccctgat aattatgctc
     2101 gaggtatgaa aaagaataga acagaaacta tcgctttgat tattccgagc atatggcatc
     2161 catttttttc ggagtttgcc tatcatgttg agaaagagtt attaaaatat aattataaat
     2221 tatttatttg taacgctgat agcgattcac aaaatgaaat cgaatatatc gaaatgttga
     2281 aacaaaataa agtggatgga attattggta ttacatattc tgatattgac aaatatattt
     2341 tatctgattt accgtttgta agtatagata gacatttttc ggaaaatgta tgctatgtta
     2401 cttcagcaaa ttacaaagga ggacaaactg cagcagagga actggttaga agggataata
     2461 agcaccttgc ttttattggt tcaattaata tgtatgagaa tgagactata ctcagaagta
     2521 aaggattcag agaaaaagct aaggaattag gagtagatgt ctataatttt gaaatgaaag
     2581 aacctttaat aaatcctgaa gatcagataa gaattttttt gcaatctcat aaagaaattg
     2641 atggtatatt tacaataaat gatatgatgg ctctgagagt tattagtgtt atgaaagaac
     2701 tggggaaaat agcacctgat gattaccaga taattgggtt tgatggttta aaaacttctg
     2761 tcgatcaatc ttatctagtg tctacaatag ttcaagatat tggtatgatg gcaaaagaat
     2821 ctgttgcaat gatactgaaa atgattaagc gagaagagat tctagaaaaa cgaatcgtaa
     2881 ttcccacata tttttgggga gataaaacaa caaaaaaaat ttaaaaagcg cttgacaatt
     2941 aatgaaagcg ctgttataat ataatcaaca aatgaaacgt ttcaaaaaat aggctagaag
     3001 cttgtttaaa aaataaataa aaaatgaaac gtttcaaaaa ggaggaaaat gatgcgtaaa
     3061 ggtacaatgt tatctgttgt tgcggggcta tcgttagcta tacttgcagg ttgtagtgga
     3121 ggaactaact caaaacaagc gagttcttca aatgatatta aagtctgggt acaattctct
     3181 gacgaaacaa atgaaggaaa agcttggcaa aaagttgttg ataactttaa tcaatcagga
     3241 aaaagtaagt acaaggttgt aactgagtat attcctcgta gtggtagtgg tggaggatat
     3301 gaagacaagg ttaatgctgc tattacgact aatagtcttc cagatgttat tacccttgat
     3361 ggaccaaata cggcagctta tgcaaaatct aaagtaatta ctccgctaga tgattatcta
     3421 aaagataata atatggatga tgttttggac agtattaaac aacagggaac ttataatggg
     3481 aaattctatg catttggatt ttctgaatcg aatgttggtg tttattacaa taaaaaaatg
     3541 ttcaaagagg ccggaattgc ggaatcagag cttccaacgc tagaaaagcc atggacatgg
     3601 gacgaattta atacgattgc taaaaaattg aaagatcatt ataacaaacc agcaattgat
     3661 tttcgtatta attcaaacga tgaaatgttg ccatatgctt atatgccgtt gatttggtca
     3721 aataatggtt ctgtcgtaaa cgaagatgga acaaaggcag aaggatactt caactccaaa
     3781 gagagtgtag aagcagttca attcattcaa aatcttgtaa aagaaggcta tacaactgtt
     3841 agtccagtcg aaaaaggttt tgaaacagga gagtatccaa tgcttttgag tggttcttgg
     3901 acgattgctg acatgaatac aaactacaaa gatattgatt ttggtatcct tccttaccca
     3961 gtatctagta agacgaaaaa attagtatcc ccttcaggta gttggcaatt agctgtgacg
     4021 acaaaatctg ataaaaaaga ggccgcatct gaatttgtta aatttgctac aaatacagaa
     4081 tcaagtgaaa ttattagctt ggggaattcg gtccttccaa ttcgtaaatc tacaattgaa
     4141 aatatcaaag ataaagtatc cgaaccaatg cgtttcttga tggaacaaaa ttcaaaaaca
     4201 gctcatgctc gcccagttgt tgtagcatat cctcaggttt ctcgagcttt tcaacaagca
     4261 atgcaagata ttagctatta tgaagaaaat ccaaatgtgc aaaaagtctt ggatactcgt
     4321 acaaaagaaa tgcaaactgc tattgatcag tcactcaaat aatctgtaaa aaagaaggtg
     4381 gaaggaaaat gtttggaact accttcccct ttcttgttaa tagcctagtt tgatatatat
     4441 gattaaagga gatggatatg agtaaagaaa ctgttgtgga tacaaaaaga cgtgaaaaaa
     4501 taaaggataa catattaggt tatagttttt tagctccagc tttaatttta ttaggtatct
     4561 ttttagtaat tccagttgga atggtcattt attacgcttt tacagattat tatttgttga
     4621 caccagatga aagaaaattt gttggacttg aaaattttat tcgattaaca aaagatccga
     4681 ttttcttaaa gagcttcttg aatactttga aatttgttgt ttggattatt ccagttcaat
     4741 tgggagcggc cttaggaatg gctttgattg tcaataaaaa gcgaaaagga aatatgtttt
     4801 ttaaagtggc tttttttgct ccagttgtca tgtccttggt agttatttct attctttggc
     4861 tttatctttt aaatccaaat agtggcttat taaatgctat tttaaataag gtaggtattg
     4921 cttctcaacc tttcttgact agtcctaaac aagctatgta tgcaattgtt tttgtttctg
     4981 cttggcaggg tgcaggttat cagatgcttt tatttttagg aggaatgcag aatattccac
     5041 aagatgtcta tgaagctgct gaattagatg gattctcaaa atgggctcag tttcgttata
     5101 tcacgatgcc cctactaaaa ccaacagccc tgtttgtatt gttaacaact ttaatttctg
     5161 cttttaagct gattgtacaa ccaatggtta tgacgcaagg tgggccatta aactcaacaa
     5221 ttactatggt gtactatatt tatcaacaag gttttactga tcgtcttgta ggatattcta
     5281 gctcaatcgc tttagtattt acaacgttaa ttggtatgat tagcttagta caacgtcgcg
     5341 ttctgaagga ggatgactaa gatgaaacga tttaaaccaa caacaatctt agaatatatt
     5401 ctgttaactt tattggcagg actcttcctt tttcctatgg tttggatggt agtttcagct
     5461 atgaagccag aagcggatgt ttataaaaat ttgactagtt ggcaagcctt cttaccaagt
     5521 ttaaatcccg ctgattggtt taaaccttat caggaagtat ttgagcgctt tagtattttt
     5581 acatatctag ggaacagcat tttttatgct ggaacgtttg ctattggttc tattattgtc
     5641 aatgcactgg ccggtttcgc ttttgcaaaa gtcaattttt caggtaagaa aattttattt
     5701 ggtttcttac ttgctttact tattattcca gtggaaactg tgttaattcc acaatttacc
     5761 attatcaatt ccttaggttt ggtcaataat cgtcttgcag ttattattcc aggattggcg
     5821 agtgtattta atatttactt atttaggaat ttctttattg caattccaga agagattctt
     5881 gagtctgcaa agatggacgg agcaagtatc gttagaattt tctttagaat tatgcttcca
     5941 atgtctaaac ctgcagttgc tacagttggt gttttatcat ttatttcaag ctggaatgat
     6001 tatatctggc cattgatggt tttgacagat tcttctaaat tttcaatgca ggttgcaatt
     6061 acaactatta atacaactca accagtttat atcaatcagg taatggctgt attaactatt
     6121 tcaacgattc cgttgatttt ggtctatgta gtcgctcaga aatatattgt tcaaggtctg
     6181 ggtggttccg gaactggaat aaaataggat atacttatga taaataaaac atttacagta
     6241 gaaaaagcca atcgttttat agcagaaaat aaacatctcg ttaatactca atataagcct
     6301 gaagaacatt tttcagctga gattggttgg atcaatgatc caaatggatt tgtttatttt
     6361 cgtggagaat accatctctt ttatcaattc tatccatatg atagtgtttg gggacctatg
     6421 cactggggac atgctaaaag taaggacttg gtgacttggg agcacttgcc agtggcactt
     6481 gctcctgatc aagattatga ccgaaatggt tgtttctcag gctctgccat tgtcaaggat
     6541 gatcgcctct ggctcatgta cactgggcat atcgaagaaa aaaccggtgt ccgccaagtg
     6601 caaaatatgg cattttcaga cgacgggatt cactttgaaa agattgaaca aaatccagtt
     6661 gcaacaggag cagacttgcc agatgagttg attgctgctg atttccgtga tccaaaactc
     6721 tttgaaaaag atggacgcta ttactccgta gtagctacca aacacaagga taatgtgggc
     6781 tgtatcgttc tactagggtc cgataaccta gtagaatggc agttcgaatc catcttttta
     6841 aaagggagag aacaccaagg ctttatgtgg gaatgcccag attacttcga gttagatggg
     6901 aaagattgcc ttattatgtc acccatgcgt tatcagcgtg agggagactc atatcataac
     6961 atcaactcat cgcttttgtt cacgggtaag gtagattggg gagaaaaacg ttttatccca
     7021 gaatcagttc aagaaatcga tcatggccaa gacttctatg cgcctcaaac attgttggac
     7081 gatcaaaatc gtcgtatcct gattgcttgg atgcagacat gggggcgtac ccttccaacc
     7141 catgaccaag aacacaaatg ggcatgtgcc atgactttac ctagaattct aagattggaa
     7201 gatggcaaac taagacaatt ccctgttaaa aaaggccaat atcaaatcca aatagataaa
     7261 gattgtcatt accacttagg aaatgataca gattatcttg aatttggtta tgacagtaat
     7321 gcgcagcaag tttacattga tcgtagccat cttattcaaa aaattctagg tgaagaagaa
     7381 caggacacta gtcgacggta tgtagatatt gaagctaaag aattggaagt tgttctagat
     7441 aaaaattcca tcgagatttt tgtcaatcaa ggtgaagcaa gcttgactgc aacttattac
     7501 ttaacggtgc cagctgagct atcacgaatt gattaaaaat taagttattt ctcctaaaga
     7561 aaaagttctc tttctaaaat agtggaaaga ggactttttg tgttttgggt atataagctt
     7621 agtttatggt atttgtaaaa ttggtgttgg attatgattt aagctagttt tctaaagaat
     7681 ttgaaaaaaa ttttatttaa gcaaaaaaac cttggttcca aggcttttcc tgttgtattt
     7741 agatgccccc tacaggaatt gtaggagata tgttgtttag atgttcttga ttttctggtg
     7801 ttttgtaacg tttaaatgag ttttttgagt ttgttggtgg ggcgttgccc ggcaattgcc
     7861 cgacttattg tttgaaaaag aatttaaaat atggtatagt taattataga ttaacacttg
     7921 gggggaatag atgaagaata atgaaagatt aggtattaaa ttaagtagag atagcgtttt
     7981 aggattgagg gaagttagaa ggctttattt aggcagttca gatatcccag tgcctgatgg
     8041 gtatgtgatt gaagttgctt ataaacagat atcaaatgag attgatatta ttgattgggt
     8101 agagttgaac aagtcaaaaa ttaagataag tgaaattagt gaaagcgtag atatagatgc
     8161 cactagcttg agaacaactt tgactttaga cacgttagta tatgaaggta tgagagatat
     8221 acagttaaag ttgagagagc ttacaaaggg gagagtattc ttttcatttg tagtgaagtt
     8281 agttttattt gcttctattt taaagaaaaa aggtttacta gaaaaatttc aagaaaagtg
     8341 ttaatcaagt attgactttt tagttgagtt caagtatact ttaattagag agatttctct
     8401 ctaaattttt tcgcttcttt tgtgttaatc aatgacaaac tctttgtctt attttttaac
     8461 gcttgggtgt gttaatcaaa gatgtatata ttggaggatg aagatgaaag ctagagcttc
     8521 accgttgaat tgattttgtt tctagaaaaa atcaataatt agaaaaaata agaggtaaga
     8581 aatgaataga aatgtactag agtttctgaa aacagagaca gctgagaaaa ttagcctttt
     8641 catcaggaaa ataaatggat tagaaggaaa tgtgacatta ctttctatta actctcaaga
     8701 tttggaagat attaaaaatg ctatgctttc caattctaat ctcggattaa aaattgcgag
     8761 actggatgtg atgaagaaaa tagcatatgc ttctaatcgt actcattata aagatggaac
     8821 gactatcatg gatgacatct catctggtaa aatacatcgt cgccctaaaa gctatatcta
     8881 ataaggaagg ttaatccctc ttccttatta gatatttaaa aaaaggagaa aaatatgctt
     8941 aagaattttt gtcaaaatgc ctttattagt ttatatttca tagtgtctct gctaggagga
     9001 ttttcagctc aggttgacct aaaatccttc tctccttatc aatctatgaa gatagccttg
     9061 ttattaggag ttgctttaat tttattgcaa cttccaataa tgtttgctaa attattagaa
     9121 gctttaaaaa ccaagaaagg tgaaactcat gaatgacatc gtaaaaatga gattagcctt
     9181 ttgtatcact ataatattga tggcatgctc tgtttttgtg tttactcctg ttggagaagg
     9241 catgtcccta ctttgctcaa tttttgctac cccgttttta tttatttttg ttttctattt
     9301 ttttaaaata aaattaagaa ttttacttat tattatcaag g
//
LOCUS       DAVKWZ010000070         9208 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000070 DAVKWZ010000000
VERSION     DAVKWZ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9208
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.070"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            201..2183
                     /locus_tag="VW854_001843"
     CDS             201..2183
                     /locus_tag="VW854_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV0263034.1"
                     /translation="MKSSKLFALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDEQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHIDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNIFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVVIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDETTDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
     gene            2483..7786
                     /locus_tag="VW854_001844"
     CDS             2483..7786
                     /locus_tag="VW854_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV0263035.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKEDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKSPTTSTWYRGSRVAAPETGSINRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGTYGNDRTVVSVKTDNQEGVKTEDTPAEKETGPEVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKNVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRDNATVNDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQAT
                     YFDEIRTFENHSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVADD
                     FASLTAPAQAQEGLANAFDGNLSSLWHTSWGGGDVGKPATMVLKEATEITGLRYVPRG
                     SGSNGNLRDVKLVVTAESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKSDAPTVEKPEFKLSSLASEQGKTPDYKQEID
                     RPETPEQILPATGESQSDTALFLAGVSLALSALFVVKTKKD"
     gene            8064..>9208
                     /locus_tag="VW854_001845"
     CDS             8064..>9208
                     /locus_tag="VW854_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1167A family transposase"
                     /protein_id="HEV0263036.1"
                     /translation="MEQLHFITKLLDIKDPNIKILDIINMDTHKEIIAKLDYEAPSCP
                     DCGSLMKKYDFQKPSKIPYLETTGMPSRILLRKRRFKCYHCSKMMVAETPLVKKNHQI
                     PRIINQKIAQKLIEKISMTDIAHQLAISTSTVIRKLNDFHFEHDFSRLPKIMSWDEYA
                     FTKGKMSFIAQDFDNLNIITVLEGRTQAVIRNHFLRYDRAVRCQVKIITMDMFSPYYD
                     LAKQLFPCAKIVLDRFHIIQHLSRAMSRFRVQIMNQFERKSHEYKAIKRYWKLIQQDS
                     RKLSDKRFYRPTFRMHLTNKEILDKILSYSEDLKHHYQIYQLLLFHFQNKDPEKFFGL
                     IEDNLKQVHPIFQTVFKTFLKNKEKIVNALQLPYSNAKLEATNNLIKL"
BASE COUNT         3028 a         1772 c         1935 g         2473 t
ORIGIN      
        1 aaatctccag attaggaact atccgtgagt tctctagtct ggagattttt caatatactt
       61 cgttattggg cgcttacaat gatattcata ttttttgcaa agatgttgtt tgaaaaataa
      121 ttttcaaaaa ttctgaaaat tctgttgaca actttctgaa aagagtctat aatggagaga
      181 aagttttaaa ggagaaaatg atgaaaagtt caaaactatt tgcccttgcg ggcgtgacat
      241 tattggcggc gactacttta gctgcatgct ctggatcagg ttcaagtact aaaggtgaga
      301 agacattctc atacatttat gagacagacc ctgataacct caactatttg acaactgcta
      361 aggctgcgac agcaaatatt accagtaacg tggttgatgg tttgctagaa aatgatcgct
      421 acgggaactt tgtgccgtct atggctgagg attggtctgt atccaaggat ggattgactt
      481 acacttatac tatccgtaag gatgcaaaat ggtatacttc tgaaggtgaa gaatacgcgg
      541 cagtcaaagc tcaagacttt gtaacaggac taaaatatgc tgctgataaa aaatcagatg
      601 ctctttacct tgttcaagaa tcaatcaaag ggttggatgc ctatgtaaaa ggggaaatca
      661 aagatttctc acaagtagga attaaggctc tggatgaaca gacagttcag tacactttga
      721 acaaaccaga aagcttctgg aattctaaga caaccatggg tgtgcttgcg ccagttaatg
      781 aagagttttt gaattcaaaa ggggatgatt ttgccaaagc tacggatcca agtagtctct
      841 tgtataacgg tccttatttg ttgaaatcca ttgtgaccaa atcttctgtt gaatttgcga
      901 aaaatccgaa ctactgggat aaggacaatg tgcatattga caaagttaaa ttgtcattct
      961 gggatggtca agataccagc aaacctgcag aaaactttaa agatggtagc cttacagcag
     1021 ctcgtctcta tccaacaagt gcaagtttcg cagagcttga gaagagtatg aaggacaata
     1081 ttgtctatac tcaacaagac tctattacgt atctagttgg tacaaatatt gaccgtcagt
     1141 cctataaata cacatctaag accagcgacg aacaaaaggc atcgactaaa aaggctctct
     1201 taaacaagga tttccgtcag gctattgcct ttggttttga tcgtacagcc tatgcctctc
     1261 agttgaatgg acaaactgga gcaagcaaaa tcttacgtaa tatctttgtt ccaccaacat
     1321 ttgttcaagc agatggtaaa aactttggcg atatggtcaa agagaaattg gtcacttatg
     1381 gggatgaatg gaaggatgtt aatcttgcag attctcagga tggtctttac aatccagaaa
     1441 aagccaaggc tgaatttgct aaagctaaat cagccttaca agcagaaggt gtgactttcc
     1501 caattcattt ggatatgcca gttgaccaga cagcaactac aaaagttcag cgcgtccaat
     1561 ctatgaaaca atccttggaa gcaactttag gagcggataa tgtagtcatt gatattcaac
     1621 aactacaaaa agacgaagta aacaatatta catattttgc tgaaaatgct gctggcgaag
     1681 actgggattt atcagataat gtcggttggg gtccagactt tgccgatcca tcaacctacc
     1741 ttgatatcat caaaccatct gtaggagaaa gtactaaaac atatttaggg tttgactcag
     1801 gggaagataa tgtagctgct aaaaaagtag gtctatatga ctacgaaaaa ttggttactg
     1861 aggctggtga tgagactaca gatgttgcta aacgctatga taaatacgct gcagcccaag
     1921 cttggttgac agatagtgct ttgattattc caactacatc tcgtacaggg cgtccaatct
     1981 tgtctaagat ggtaccattt acaataccat ttgcattgtc aggaaataaa ggtacaagtg
     2041 aaccagtctt gtataaatac ttggaacttc aagacaaggc agtcactgta gatgaatacc
     2101 aaaaagctca ggaaaaatgg atgaaagaaa aagaagagtc taataaaaag gctcaagaag
     2161 atctcgcaaa acatgtgaaa taactgttgc aaaatataag aaaggattta gtatttctct
     2221 tgaatgctga atcctttttt acatttgtaa agaaagattc taaatgtact gacccccaaa
     2281 agttggagcc tctttttgtc agaatagaga aaatttttgt taattttact tgtttcctat
     2341 tgctttctca gttattattt gttatattaa aagtataatt atttttattt atcagggtta
     2401 agcattgcac tttcagagga aggagtattt tttaaaaaga aaatgtaaac gcttgctaaa
     2461 aaatgaaagg atttagaagt ttatgaataa aggattattt gaaaaacgtt gtaaatatag
     2521 tattcggaaa ttttcattag gtgttgcttc tgttatgatt ggagctgcat tctttgggac
     2581 aagtccggtt cttgcagata gcgtgcagtc tggttccacg gcgaacttac cagctgattt
     2641 agctactgct cttgcaacag caaaagagaa tgatgggcgt gattttgaag cgcctaaggt
     2701 gggagaagac caaggttctc cagaagttac agatggacct aagacagaag aagaactatt
     2761 agcacttgaa aaagaaaaac cggctgaaga aaaaccaaaa gaggataaac ctgcagctgc
     2821 taaacctgaa acgcctaaga cggtaacccc tgaatggcaa acggtagaga aaaaagaaca
     2881 acagggaaca gtcactatcc gagaagaaaa aggtgtccgc tacaaccaac tatcctcaac
     2941 tgctcaaaat gataacgcag gtaaaccagc cctgtttgaa aagaagggct tgaccgttga
     3001 tgccaatgga aatgcaactg ttgatttaac cttcaaagaa gattctgaaa agggcaaatc
     3061 acgctttggt gtcttcttga aatttaaaga taccaataat aatgtttttg tcggttatga
     3121 caaggatggc tggttctggg agtataaatc tccaacaact agcacttggt atagaggtag
     3181 tcgtgttgct gctcctgaaa caggatcaat aaaccgtctc tctatcactc tcaagtcaga
     3241 cggtcagcta aatgccagca ataacgatgt caatctcttt gacacagtga ctctaccagc
     3301 tgcggtcaat gaccatctta aaaatgagaa gaagatcctt ctcaaggcag gaacttatgg
     3361 caatgaccgt acggttgtca gcgttaaaac ggataaccaa gagggggtaa aaacagagga
     3421 tacccctgct gaaaaagaaa caggtcctga agttgatgat agcaaggtga cttatgacac
     3481 gattcagtct aaggtcctca aagcagtgat tgaccaagcc ttccctcgtg tcaaggaata
     3541 cagcttgaac gggcatactt tgccaggaca ggtgcaacag ttcaaccaag tctttatcaa
     3601 taaccaccga atcacccctg aagtcactta taagaaaatc aatgagacaa cagcagagta
     3661 cttgatgaag cttcgcgatg atgctcactt aatcaatgcg gaaatgacag tacgcctgca
     3721 agttgtggac aatcaattgc actttgatgt gaccaagatt gtcaaccaca atcaagtcac
     3781 tccaggtcaa aagattgatg acgaaagaaa actactttct tctattagtt tcctcggcaa
     3841 tgctttagtc tctgtttcta gtgatcaaac tggtgctaag tttgatgggg caaccatgtc
     3901 aaacaatacg catgtcagcg gagatgatca tatcgatgta accaatccaa tgaaagacct
     3961 agccaagggt tacatgtatg gatttgtttc tacagataag cttgctgctg gtgtttggag
     4021 taactctcaa aacagctatg gtggtggttc gaatgactgg actcgtttga cagcttataa
     4081 agaaacagtc ggaaatgcca actatgtagg aatccacagc tctgaatggc aatgggaaaa
     4141 agcttataaa ggcattgttt tcccagaata cacgaaggaa cttccaagtg ctaaggttgt
     4201 tatcactgaa gatgccaatg cagacaagaa cgttgattgg caagatggtg ccattgctta
     4261 tcgtagcatt atgaacaatc ctcaaggttg ggaaaaagtt aaggatatca cagcttaccg
     4321 tatcgcgatg aactttggtt ctcaagcaca aaacccattc cttatgacct tggatggtat
     4381 caagaaaatc aatctccata cagatggtct tgggcaaggt gttctcctta aaggatatgg
     4441 tagcgaaggc catgactctg gtcacttgaa ctatgctgat attggtaagc gtatcggtgg
     4501 tgtcgaagac ttcaagaccc taattgagaa ggctaagaaa tatggagctc atctaggtat
     4561 ccacgttaac gcttcagaaa cttatcctga gtctaaatac ttcaatgaaa aaattctccg
     4621 taagaatcca gatggaagct atagctatgg ttggaactgg ctagatcaag gtatcaacat
     4681 tgatgctgcc tatgacctag ctcatggtcg tttggcacgt tgggaagatt tgaagaaaaa
     4741 acttggtgac ggtctcgact ttatctatgt ggacgtttgg ggtaatggtc aatcaggtga
     4801 taacggtgcc tgggctaccc acgttcttgc taaagaaatt aacaaacaag gctggcgctt
     4861 tgcgatcgag tggggccatg gtggtgagta cgactctacc ttccatcact gggcagctga
     4921 cttgacctac ggtggctaca ccaataaagg tatcaacagt gccatcaccc gctttatccg
     4981 taaccaccaa aaagatgctt gggttgggga ctacagaagt tatggtggtg cagccaacta
     5041 tccactgcta ggtggctaca gcatgaaaga ctttgaaggc tggcaaggaa gaagtgacta
     5101 caatggctat gtaaccaact tatttgccca tgacgtcatg accaagtatt tccaacactt
     5161 cactgtaagc aaatgggaaa atggtacacc ggtgactatg accgataacg gtagcaccta
     5221 taaatggact ccagaaatgc gagtggaatt ggtagatgct gacaataata aagtagttgt
     5281 aactcgtaag tcaaatgatg tcaatagccc acaatatcgc gaacgtacag taacgctcaa
     5341 cggacgtgtc atccaagatg gttcagctta cttgactcct tggaactggg atgcaaatgg
     5401 taagaaactt tctactgata aggaaaaaat gtactacttc aatacgcagg ctggtgcaac
     5461 aacttggacc cttccaagcg attgggcaaa gagcaaggtt tacctttaca agctaactga
     5521 ccaaggtaag acagaagagc aagaactaac tgtaaaagat ggtaaaatta ccctagatct
     5581 tctagcaaat caaccatacg tactttaccg ttcaaaacaa accaatcctg aaatgtcatg
     5641 gagtgaaggc atgcacatct atgaccaagg atttaatagt ggaaccttga aacactggac
     5701 catttcaggc gatgcttcta aggcagaaat tgtcaagtct caaggggcaa acgatatgct
     5761 tcgtattcaa ggaaacaaag aaaaagttag tctcactcag aaattaactg gcttgaaacc
     5821 aaataccaag tatgccgttt atgtcggtgt cgataaccgt agtaatgcca aggcgagcat
     5881 cactgtaaat actggtgaaa aagaagtgac tacttatacc aataagtctc tcgcgctcaa
     5941 ctatgttaag gcttacgccc acaatacacg tcgtgacaat gctacagtta acgatacaag
     6001 ttacttccaa aacatgtacg ccttctttac aacaggatcg gacgtctcaa atgttactct
     6061 gacattgagt cgtgaagctg gtgatcaagc aacttacttt gatgaaattc gtacctttga
     6121 aaaccattca agcatgtatg gagacaagca tgatacaggt aaaggcacct tcaagcaaga
     6181 ctttgaaaat gttgctcagg gtatcttccc atttgtagtg ggtggtgttg aaggtgttga
     6241 agataaccgc actcacttgt ctgaaaaaca tgatccatat acacaacgtg gttggaacgg
     6301 taagaaagtt gatgatgtta tcgaaggaaa ttggtcactc aagacaaatg gactagtgag
     6361 ccgtcgtaac ttggtttacc aaactattcc gcaaaacttc cgttttgaag caggtaagac
     6421 ctaccgtgta acctttgaat acgaagcagg ttcagacaat acctatgctt ttgtagtcgg
     6481 taagggagaa ttccagtcag gtcgtcgtgg tactcaagca agcaacttgg aaatgcatga
     6541 attgccaaat acttggacag attctaagaa agccaagaag gcaaccttcc tcgtgacagg
     6601 tgcagaaaca ggggatactt gggtaggtat ctactcaact ggcaatgcaa gtaatactcg
     6661 tggtgattct ggtggaaatg ccaacttccg tggttataac gacttcatga tggataatct
     6721 tcaaatcgaa gaaattaccc taacaggtaa gatgttgaca gaaaatgctc tgaagaacta
     6781 cttgccaacg gttgccatga ctaactacac caaagagtct atggatgctt tgaaagaggc
     6841 ggtctttaac ctcagtcagg ccgatgatga tatcagtgtg gaagaagcgc gtgcagagat
     6901 tgctaagatt gaagccttga agaatgcttt ggttcagaag aaaacggctt tggtagcaga
     6961 tgactttgca agtcttacag ctcctgctca ggctcaagaa ggtcttgcaa atgcctttga
     7021 tggaaactta tctagtttat ggcatacatc atggggcgga ggagatgtag gcaagcctgc
     7081 aaccatggtc ttgaaagaag caactgaaat cactggactt cgttatgttc cacgtggatc
     7141 aggttcaaat ggtaacttgc gtgatgtgaa acttgttgtg acagctgagt ctggcaagga
     7201 gcataccttt actgcaactg attggccaga taacaataag ccaaaagaca ttgattttgg
     7261 taagacaatt aaggctaaga aaattgtcct tacaggtact aagacttacg gagatggtgg
     7321 cgataaatac caatctgcag cggaacttat ctttactcgt ccacaggtag cagaaacacc
     7381 tcttgacttg tcaggctatg aagcagcttt ggctaaggct cagaaattaa cagacaaaga
     7441 caatcaagag gaagtagcta gcgttcaggc aagcatgaaa tatgcgacgg ataaccatct
     7501 cttgacggaa agaatggtgg aatactttgc agattatctc aaccaattaa aagattctgc
     7561 tacgaaatca gatgctccaa ctgtagagaa acctgagttt aaacttagct ctttagcttc
     7621 cgaacaaggt aagacgccag attataagca agaaatagat agaccagaaa cacctgaaca
     7681 aatcttgcca gcaacaggtg agagtcaatc tgacacagcc ctcttcctag caggtgttag
     7741 cctagcccta tctgctctct ttgtagtaaa aacgaagaaa gactagtatt tagtaaaacc
     7801 tcttaacaag attacggaag cagtctctat cttttccaat gaggtttata gtacagaaaa
     7861 agcctgagaa gatgtcttct caggcttttg ttaagcacat aaatacaata gtgctatgaa
     7921 aaaatcaccc agaaaaatct ggctctataa tatctatagt gggtaaatcc actatagata
     7981 ttatggagcc tattttattg tagaaaaaaa gtcccatatg acctataatg aaaagcgaca
     8041 aaaccatcat tagaaagatt catatggaac aattacattt tatcacaaaa ctgctcgata
     8101 ttaaagaccc aaacatcaag attctagata tcatcaatat ggatacccac aaagaaatta
     8161 tcgctaagct ggattatgag gctccatctt gccctgattg tggaagtcta atgaagaaat
     8221 atgactttca aaaaccgtct aagatccctt acctcgaaac aactggtatg ccttctagaa
     8281 ttctccttag aaaacgccgt ttcaagtgct atcactgttc aaaaatgatg gttgctgaga
     8341 ctcccctggt aaagaaaaat caccaaatcc ctcgtatcat caaccaaaaa attgctcaaa
     8401 agttaattga aaaaatttct atgactgata ttgcccatca gctggccatt tcaacttcaa
     8461 ctgtcattcg caagctcaat gactttcact ttgagcatga tttttctcgg cttccaaaga
     8521 ttatgtcttg ggatgagtat gccttcacta agggaaagat gagtttcatt gcgcaagatt
     8581 ttgataatct taatattatc actgttcttg aaggcagaac acaagctgtc atccgaaatc
     8641 actttcttcg ctacgataga gccgttcgtt gtcaagtgaa aatcattacg atggatatgt
     8701 ttagtcctta ctatgacttg gctaaacagc tttttccgtg tgctaaaatc gttctagatc
     8761 gtttccatat tatccaacat ctcagccgtg ccatgagtcg ttttcgtgtt caaattatga
     8821 atcagtttga acgaaaatct catgaataca aggctatcaa acgttactgg aaactcatcc
     8881 aacaggatag tcgtaaattg agcgataaac gtttttatcg ccctactttt cgcatgcact
     8941 taacaaataa agaaattctt gacaagattt taagctattc agaagacttg aaacaccact
     9001 atcagatcta tcaactctta ctttttcact ttcagaacaa agaccctgag aaatttttcg
     9061 gactcattga ggacaatctg aagcaggttc atcctatttt tcagactgtc tttaaaacct
     9121 ttctaaagaa caaagaaaaa atcgtcaacg ctcttcaatt accttattcc aacgcaaaat
     9181 tggaagcgac caataatctc atcaaact
//
LOCUS       DAVKWZ010000071         8675 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000071 DAVKWZ010000000
VERSION     DAVKWZ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8675
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.071"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            75..425
                     /locus_tag="VW854_001846"
     CDS             75..425
                     /locus_tag="VW854_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEV0263037.1"
                     /translation="MLRHPGISPTNDLIAKKIFSNPEITCQFIRDMLDLPAKNVTILE
                     GSDIHVLLSMPYSAQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWTYLCSQVN
                     QNLEKIRQREGDTH"
     gene            640..756
                     /locus_tag="VW854_001847"
                     /pseudo
     CDS             640..756
                     /locus_tag="VW854_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
     gene            749..982
                     /locus_tag="VW854_001848"
     CDS             749..982
                     /locus_tag="VW854_001848"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263038.1"
                     /translation="MFSQLHMREEQVLLAQDYALETARAEGLEQGLERGKVEGRAERK
                     LFAFLDIVRQGLLTSEVASQQLGMSVSEFEALL"
     gene            1109..1387
                     /locus_tag="VW854_001849"
                     /pseudo
     CDS             1109..1387
                     /locus_tag="VW854_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1524..2492)
                     /locus_tag="VW854_001850"
     CDS             complement(1524..2492)
                     /locus_tag="VW854_001850"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV0263039.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(2637..3260)
                     /locus_tag="VW854_001851"
     CDS             complement(2637..3260)
                     /locus_tag="VW854_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEV0263040.1"
                     /translation="MPKKVLILSLLGGMFLSGWLSSFANTYIHDLLGVLFPDSTFLNA
                     FESAIVAPLVEEPLKLLPLVFVLALIPVRKLKSLFLLGIASGLGFQMIKDIGYIRTDL
                     PEGFDFTISRILERIISGIASHWTFSGLAVVGVYLLYRAYKGQKVGKKQGLIFLGLAL
                     GTHFLFNSPFVELETELPLAIPVVTAIAFYGFYHAYCFVEKHNELMT"
     gene            complement(3242..3451)
                     /locus_tag="VW854_001852"
     CDS             complement(3242..3451)
                     /locus_tag="VW854_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263041.1"
                     /translation="MKKSSILFIFILLLCIGLQYETIYYTDGSRSGAEYGLMGVSIFL
                     ALFYMIPALYFLFRIGKNGNCQRRF"
     gene            complement(3476..3973)
                     /locus_tag="VW854_001853"
     CDS             complement(3476..3973)
                     /locus_tag="VW854_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEV0263042.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(4046..5407)
                     /gene="radA"
                     /locus_tag="VW854_001854"
     CDS             complement(4046..5407)
                     /gene="radA"
                     /locus_tag="VW854_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEV0263043.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(5421..5936)
                     /locus_tag="VW854_001855"
     CDS             complement(5421..5936)
                     /locus_tag="VW854_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV0263044.1"
                     /translation="MKIIFVRHGEPDYSELEERSYIGFGINLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(5938..6381)
                     /locus_tag="VW854_001856"
     CDS             complement(5938..6381)
                     /locus_tag="VW854_001856"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEV0263045.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            complement(6438..6535)
                     /gene="ffs"
                     /locus_tag="VW854_001857"
     ncRNA           complement(6438..6535)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VW854_001857"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(6568..7035)
                     /locus_tag="VW854_001858"
     CDS             complement(6568..7035)
                     /locus_tag="VW854_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEV0263046.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            complement(7236..8522)
                     /locus_tag="VW854_001859"
     CDS             complement(7236..8522)
                     /locus_tag="VW854_001859"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEV0263047.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
BASE COUNT         2734 a         2088 c         1485 g         2368 t
ORIGIN      
        1 ataaattaat ttgactttcc tgatagagtt attcacatct tatttcaact cactatagaa
       61 ggaggaatag gaggattctc agacatccgg gcatcagccc aactaatgat ttgattgcta
      121 agaaaatatt cagcaatcca gaaatcactt gtcaatttat tcgcgatatg ctggacttgc
      181 cagcaaaaaa tgtgaccatt ttggagggaa gcgatattca cgtattactc tccatgcctt
      241 actcagcgca ggatttttat accagtatag acgtcttggc ggagttggat aacggtactc
      301 aagtaattat tgagattcaa gtccatcatc agaatttttt catcaatcac ttgtggactt
      361 acctgtgcag tcaggttaat caaaatcttg aaaaaattcg tcagcgagaa ggtgatactc
      421 actagagcta caaacacatc gctcctgttt acgccattgc tatcgtggat agtaattatt
      481 tctcagatga cctggctttt catagcttta gtatgcgcga agacacaaca ggtgaggtat
      541 tggcgattac caacaatgga caggaaaacc atctggttaa gatggcattc ttggaattaa
      601 aaaatacaga gaaaccagca aggacgaggt tcgcaagcca tggttggagt ttttcggcaa
      661 caagcccttt acccagcaac cgcaacgagc cattagccaa gcaaatcaac tgctggacta
      721 caagagctgg tccgaggagg acaggaaaat gtttagtcaa ctacatatgc gagaagaaca
      781 agtcttgtta gcacaggact atgccttgga aactgctagg gctgaaggtc ttgaacaagg
      841 actagagcgt gggaaagttg aaggaagggc agaaaggaaa ctttttgcct tcctagacat
      901 agtacgccaa ggtcttctga cttctgaggt tgccagccag caattaggta tgtcagtatc
      961 tgaatttgag gcactgttgt aaaatggctc cataatatcc atagtgggta aatcccctat
     1021 ggatattatg gagcctattt tgtgtagaaa aaaagtccca tatgacctat aatgaaaagc
     1081 gacaaaacaa ctcattagaa agaatcatat ggaacaatta cattttatca caaaattact
     1141 agacattaaa gaccctaata tccagatttt aaacatcatc aataaggata cacacaagga
     1201 aatcatcgcc aaactggact acgacgcccc atcttgccct gagtgcggaa accaattgaa
     1261 gaaatatgac tttcaaaaac cttctaaaat tccttatctt gaaacgactg gtatgcctac
     1321 tagaattctc cttagaaagc gtcgattcaa gtgctatcac tgttcaaaaa tgatggtcgc
     1381 tgaaacttat gatgacgtac agtcatattt cttctctttt tattatatca cagttttaaa
     1441 tctagcttta ctagattcac cgctactatc tatttattcg gaaaaaagac gaaaaaacct
     1501 gagaatcatc tcaggcttgg tcattaaatt tttttctcaa tatcgaaaag tggagaaagt
     1561 ggtcgttttt catgaatacg tacgatagca tcccctagga gatgagcgat tgaaatctgc
     1621 tcaatcttat caatcaaacg ctcttctggc agatagatgg tatccaaaac aaccaatttc
     1681 ttaatagctg atttttggat attgtccgta gcaggaccag aaagaactgg gtgcgtacag
     1741 cttgcataga cttcaacagc accagcttcc gcaagagcat ctgccgcatg acaaatcgtt
     1801 ccagcggtat caatcatatc atcaatcaag atacaagtct tgccttcaac cttaccgatg
     1861 atgttcataa cttcactagt attcatctta tcaacgctac gacgtttatc aataatagcg
     1921 atagatgttt tcaaaaattc tgccaactta cgagcacgag tcacccctcc atggtccggg
     1981 ctgacaacca catagtcaga accaaccata ccacgacgct caaaataatc tgcaatcaga
     2041 ggagcaccca tcaaatgatc cacaggaata tcaaagaatc cttgaatttg cgcagcatgc
     2101 aagtcgatgg tcaataaacg atccactcca gctacttcaa gcatatttgc gacaagtttt
     2161 gaagtgattg gctcacgcgc tctcgccttt ctatcctgac gtgcataccc atagtaaggc
     2221 atgacaacat tgacagattc tgcactcgca cgcttcaaag catctaccat aatcaaaatt
     2281 tcaagcagat tgtcatttac aggcgaacta gttgattgta ggataaagac gtgtttccca
     2341 cggattgatt cttcaatgtt gacctgaatc tctccatctg aaaattggcg aacacttgat
     2401 ttccccaact ctatcccaat ctcctgcgcc acacgttctg ccaattcttt attagaagaa
     2461 agggcaaaca gctttaaatc agaaaaagac atgatttcct ccggtatata tgtataactt
     2521 gtgcttttca caagattttc catctaccat tgtagcgctt tttgcactat ttttcaatca
     2581 aaaataaaag aaggacacca tatttgtacc cttgcatcat tcttttgaaa aatattctag
     2641 gtcatcaact cattgtgttt ctcaacaaag caataagcat gataaaaacc atagaaagca
     2701 atagccgtaa ccactggaat cgctaaaggc aactctgttt ccaactccac aaaaggagag
     2761 ttaaacaaga agtgagttcc caaggctaaa cctagaaaaa taaggccctg tttcttgcca
     2821 accttctgtc ctttataggc tctgtaaagc aagtaaacac ctactacagc tagacctgaa
     2881 aaagtccagt gagaggcaat tcctgagatg atacgctcta aaattcgcga aatagtaaaa
     2941 tcaaagccct ctggcaaatc cgtacgaata taaccaatat ccttaatcat ttggaatccc
     3001 aaaccggaag caattccaag taaaaacaaa gattttaatt ttcgcacagg aatcaaagcc
     3061 aaaacaaaaa caagtggcaa taatttcaag ggttcttcta ccaaaggagc cacaatagca
     3121 ctttcaaagg catttaaaaa tgtactatct gggaaaagaa cccccagtaa atcatggata
     3181 taagtattag caaaactaga caaccagcct gaaaggaaca tccctcccaa taaagacaga
     3241 atcaaaacct tctttggcaa ttcccatttt tcccaatacg gaagagaaaa taaagagccg
     3301 gaatcatgta aaagagagct agaaagatag aaactcccat tagtccatat tccgcacctg
     3361 acctcgaacc gtccgtatag tagatggttt catactgtaa accaatacat agcaataaaa
     3421 taaaaataaa taaaatactg cttttcttca tacactttct ttctaaatga agtatttata
     3481 attctacgac tgtcatactt cctgtatcaa cattgtaaat ggcaccagag ataatgacat
     3541 cgtctggtat taggggagac tcgataagca gttgcatatc ctcgcgtaca ctctcttcta
     3601 tatcttggaa gggcaagaag tcctggtctg acacatctac acctaattcc tcttttaaat
     3661 actcctgaaa aggttcattt tcaaaggtct gagcaccaca gtctgtatgg tgcaatacca
     3721 caatctctct tgtccccatt tgttgctggg aaataactag cgaacgaatc atgtcttcag
     3781 tcactcgacc acctgcattc cgcaagatat gagcatcccc aagtgccaaa cctagagctt
     3841 gcgcaacgtg caaacgtgag tccatacagg tcacaatggc tactctggtt ttaggtttaa
     3901 gtggcagatt taactgccca tgtagggcaa cataagcctg attggcttgc ataaactgtt
     3961 caaaatacga cacgattccc tccttgaaaa tttgatagtc aaatatttct cctatcttat
     4021 catttttaag agaatttgtc acggattatg caaagacctt tttcaagact tcctgaatcg
     4081 ttgtcacgcc aatgacctga atttccttag gcagagtgat tcctgtcaag gaattcttag
     4141 gtacataaat cttagtaaag cccagtttag cagcttcgtt gatgcgttgc tcaatacgat
     4201 tcacgcgccg aatctctcct gtcaagccca gttctccgac aaaacattcc tgaggattag
     4261 ttggcttgtc tttgtagctc gaagcaatag caactgcaac agccaagtca atggcaggtt
     4321 catccaattt aacaccacca gcagatttga gataggcatc ctgattttgc aagagaagcc
     4381 ctgcccgttt ttccaaaaca gccataatca agctagcacg gttaaaatca agtcctgtcg
     4441 tagtacgctt ggcatttcca aacatggtcg gtgttaccaa agcctgaacc tccgccaaaa
     4501 tcggacgcgt cccttccatg gttacaacga tggaggaacc agtcgcccca tccaaacgct
     4561 cttctaggaa aacttgactc ggattgagta cctcaaccaa gccgcccgac tgcatctcaa
     4621 aaatcccaat ctcattagtg gaaccaaaac gatttttgac cgctctcaaa atacgaaagg
     4681 tgtggtgacg ctccccttca aagtaaagca ccgtatccac catatgctcc aacatacgag
     4741 gcccagccaa ggttccttct ttggtcacat gacctacgat aaagatggca atgttattgg
     4801 tcttggccaa ctgcatgagt tcagcggtca cttcacgtac ctgagaaaca gacccctgca
     4861 cccctgaaat ctcaggagac atgatggtct ggatggaatc aataatgaga aagtctggct
     4921 ggatacgctc cacttctgca cgaacactct gcatattggt ctctgcatag agataaaact
     4981 cactatcaat atcacctaag cgctctgcac gtagtttaat ctgctgggca gactcctccc
     5041 cactgacata gagaactgtc cccacttggg acaactgggt tgagacttgt aggagaagag
     5101 ttgatttccc aatcccagga tccccaccga tgaggacgag acttcctggt accactccgc
     5161 ctccaagcac acggttgaat tcctccatct ccgtcttggt tcgattgaca ttgatggaag
     5221 tcacctcagc tagtttcatg ggcttggttt tctcacctgt caaggacaca cgcgcattct
     5281 tgacctcggc aacctcaacc tcttccacaa aagaagacca agacccacag ttggggcaac
     5341 gtcccagata tttaggggaa ttatacccac aattttgaca tacaaatgtc gcttttttct
     5401 ttgcgatgac aaacctcttt ctatatctct aactcacact caatcacttg gcaaaaatca
     5461 atcttctcat ttggcacaaa ctggcgcatg agcattcgat gagcaacaac taccacagtc
     5521 tgatgttctc gatacttaga catacattct agaaaccgag acttcatttc cgtagctgtc
     5581 tcatattgaa taggactatt aggaagcaac tcccccttgt tttctaaaaa cagtcttcta
     5641 gctgtttcaa agttttctat tcctgtttta tagacctgcc attcatgtaa taaaggctct
     5701 actcttaaag gaagacccgt agcacagacc acatacgaag ccgtttctaa agctcttgtg
     5761 actgcagaag atacgattat ttcagctgac gagagtaaag gatttttgct caatttctgg
     5821 acttgctgcc gtcccatctc agacaagggt gccaaattta tcccaaatcc tatataagaa
     5881 cgctcctcta actcactgta atccggctcc ccatgacgta caaagataat cttcattcta
     5941 gtgccctgtc gatccaaatc caccagttcg aacgccatca gctgcatctc catctgcaat
     6001 taagaaagta gcaaaaacag cctggacaat acgctcccca acttcaagaa caacctcttg
     6061 gtctgtgata ttcttcatct gcgcaaaaat atgcccttca tttccaggat ttccataata
     6121 atccccatca atgaccccaa ctgagttaat taaaaccaag cccttcttac gaggatttga
     6181 agaacgatca tagaggtaga gaacctcagt cggctgcata taagccttaa cccctgtcgg
     6241 aaccaagaca atctctcctg gcgcaacaac tgtacgcaca gcaaccttta agtcgtaacc
     6301 agccgcatgc gctgtctcac gcttgggcaa taaattttca tctgtaaaac tcgaaaccaa
     6361 ttcaaaacca cgaattttca taattttctc ttttctatta tcatttattc tagattattc
     6421 tatcttattt attcggaaaa agcacgaaaa aaagagcaca caattcaaat cgcttagggc
     6481 tgctggattc ctcccctgac ccgcttcacg cagaactgtt gctccactat ttattatatc
     6541 acattcctat tcattttaaa agcaaaatta ttttttccgt ctatttctaa aaaaatcctg
     6601 catgatagct gcgcattcat cttccaaaat tcctatttca acctccacac gatgattgag
     6661 acgctcatct gtcaagatat cgtacaaact cccagcagcg ccaaatttct ggtttttagc
     6721 cccatagacc acatttggaa tgcgggcaag cccaatcgcc ccactacaca tgacacaagg
     6781 ttcaatggtc acaaaaagtg tgcaatccag caagcgccag ctctcctcac tcaagttcgc
     6841 atcctctata gccataattt ccgcatgcat aaccgctcgc tgtaattcct cacgcgcatt
     6901 atgcccacga ccaatgattt ccccatcttt gacaatcaca caaccaattg gaatttcatc
     6961 gtgttcaaga gcaatctcag cctctctcaa agcctccctc ataaagactt ctttttcttc
     7021 aagcgtataa tacatctctt ttctcttttc ctacttatcg attttattat tatatcatga
     7081 atcccaacac aaaaaaagcc accgaatgcg gtgactctat agggagatta ttatgaaaaa
     7141 gaaaagttta ggatatttgt tacaacaagt taggaggtct tcttgtaact gtctatagta
     7201 tacccgacct atcttaaaca aatcttaaaa atctcttagg accaaacact ttctaaaata
     7261 tttgtttgtt cacgaccagg acctactgag aaagtagaaa tacgaacgcc aaccaattca
     7321 ctcacacgac gaacatagtt acgcgcattc tcaggaagat cttccaaatt gcgaactccg
     7381 gtaatatctt ctgaccaacc tggcaactct tcatagatag gcttgcaacg tttcaattgc
     7441 tcaagactag ctggatagta gtcaatacgt tgaccgtcaa gatcataggc cacacagatt
     7501 ttcacagtat ccaaaccgct caaaacatca atagagttca aagaaaggtt agtaatacca
     7561 gaaacacgac ggctatgacg catcacaact gagtcaaacc aacctacacg acgtggacga
     7621 ccagttgttg taccatactc atgacccact tcacggatac gttctcccac ttcatcaaac
     7681 aactcagttg ggaaaggacc atctcctaca cgactcgtat aagctttaca tacacctaca
     7741 accttgtcaa tcttgcttgg accgacacca gaaccaattg tcacaccacc agctacaggg
     7801 tttgatgacg taacaaatgg ataagtacct tggtcgatat ctagcataac accttgtgca
     7861 ccttcaaaaa gcacacgttt gccattatca agcgcatcat tcaagataac agatgtatct
     7921 atcacgtatt tcttgatttg ttgaccatat tcgtaatatt cttcaaaaat atcatcgaaa
     7981 acaatcgctt tactgtcata caatttttca aaaagacgat tcttttcagc aaggttacgt
     8041 tctaaacgct cacggaaaat atctttatct aaaagatctg caatacgaat tccaacacga
     8101 gcagccttgt ccatataagc tggaccaatt cccttaattg tcgtaccaat cttattgtcg
     8161 cccttagctt cttcttgcaa gcgatccaac tcgatatgat aaggcaaaat aacatgcgca
     8221 cgatcagaaa tacgcaagtt atcagttgtt acaccttcct catgaagata gctcaactct
     8281 tttacaagag atttaggatt tacaaccata ccattcccaa tgacagatat tttttcaggg
     8341 aagaaaatcc cagatggaat caagtgcaac ttaaatttct taccgtcaat cacaatcgtg
     8401 tgaccagcat tatcaccacc ttggtaacgt gcaatcactt ctgcattcgc tgaaaggaag
     8461 tctgtaatct tccctttacc ttcatcaccc cattgggtac ctacaacaac aactgaagtc
     8521 ataattttgt ctgagccctc aggctcttcc tttctcacat acatggcagg actctcacct
     8581 gcaattatat cttacaattt attataataa aaaatcgcct ttttatcaag aagaaacaat
     8641 agaaagattt gctatttcca actattaaaa aatga
//
LOCUS       DAVKWZ010000072         8407 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000072 DAVKWZ010000000
VERSION     DAVKWZ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8407
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.072"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            <1..220
                     /locus_tag="VW854_001860"
     CDS             <1..220
                     /locus_tag="VW854_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HEV0263048.1"
                     /translation="GCLDMVVIQKQNEIEELSTNIEKKIMNYIFEDDGNVSEVIGALD
                     IIHHLDKIAHTTQSIYKWIMYRKYGNIN"
     gene            233..1708
                     /gene="galT"
                     /locus_tag="VW854_001861"
     CDS             233..1708
                     /gene="galT"
                     /locus_tag="VW854_001861"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEV0263049.1"
                     /translation="MKTIIDNFVDRVIEYGMYNEIDKIYVKNRVLALIGEEGTDRISD
                     ENDLKQIKDYLVEIALKNGKIKDLIEEKECLGAELMNFIVPLPSRLNDIFWSSYDISP
                     QEAVEEFYKLSKDSDYIKTSAIAKNIEFRASTKYGELEITINLSKPEKDPKTIAAEKL
                     VKATNYPKCLLCMENEGYQGRINYPARSNHRIIRLKLGDEVWGFQYSPYLYFNEHAIF
                     LNSQHVPMAITSKTFEQLLEIVDILPGYFAGSNSDLPISGGSILSHNHYQGGKHIFPM
                     EKAKFESEFRFKDFEDVNAGIVKWPMSVIRLQSENKNRLLDLATKILNKWREYSDLEV
                     DIIAMTEDVPHHTVTPIARKVDGRYELDIVLRDNHTTEQYPDGVFHPHQDVQHIKKEN
                     IGLIEVMGLAILPPRLKPELEEVGKYLLGEENAIADYHLEWADQLKEKYPRINKEEVN
                     NVVQHEAGQVFARVLEDAGVYKNTPSGHEAFMRFVKSVGIN"
     gene            1721..2731
                     /gene="galE"
                     /locus_tag="VW854_001862"
     CDS             1721..2731
                     /gene="galE"
                     /locus_tag="VW854_001862"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV0263050.1"
                     /translation="MAILVTGGAGYIGSHTVVELLNLGKEVIIVDNLSNSSILVLDRI
                     EAITGIRPVFYELDVCDKQALRKVFEQESIDAAIHFAGYKAVGESVQKPVMYYKNNIM
                     STLALVEVMSEFNVKKIVFSSSATVYGINNQSPLIETMQTSATNPYGYTKVMLEQILK
                     DVHVADSEWSIALLRYFNPIGAHESGLIGEDPSGIPNNLMPYIAQVAVGKLSELSVFG
                     NDYDTLDGTGVRDYIHVVDLAIGHIKALEKVSEKTDVYIYNLGSGEGTSVLQLVNTFE
                     SVNKIPIPYKIVPRRSGDVATCYANADKAYKELNWRTTKSIEDMCRDTWNWQSKNPNG
                     YN"
     gene            2911..3570
                     /locus_tag="VW854_001863"
     CDS             2911..3570
                     /locus_tag="VW854_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEV0263051.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            3595..4662
                     /locus_tag="VW854_001864"
     CDS             3595..4662
                     /locus_tag="VW854_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV0263052.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            4766..5776
                     /locus_tag="VW854_001865"
     CDS             4766..5776
                     /locus_tag="VW854_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0263053.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            5788..7479
                     /locus_tag="VW854_001866"
     CDS             5788..7479
                     /locus_tag="VW854_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV0263054.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            7482..8192
                     /locus_tag="VW854_001867"
     CDS             7482..8192
                     /locus_tag="VW854_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEV0263055.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT         2848 a         1154 c         1650 g         2755 t
ORIGIN      
        1 tggatgttta gatatggttg ttatacaaaa acagaatgag atagaagagc ttagtacaaa
       61 tattgaaaag aaaataatga attacatatt tgaagatgat gggaatgttt ctgaagtaat
      121 tggtgcttta gatattattc atcaccttga taaaattgct catactacgc aatcaattta
      181 taaatggata atgtatagaa aatatgggaa cataaattag aaagaaaaat atatgaaaac
      241 aattattgat aattttgttg atagagtaat tgaatacgga atgtataatg aaattgacaa
      301 aatatatgtg aaaaatagag tactagcgtt aataggtgag gagggaactg atcgcatttc
      361 cgatgaaaat gacctgaaac aaataaaaga ttacttagtg gaaatcgcct taaaaaacgg
      421 taaaattaaa gatttaatcg aggaaaagga atgtttagga gcagagttaa tgaatttcat
      481 agttccttta cctagtcgat taaatgatat tttttggagt tcatatgata tatcacctca
      541 agaagcagtt gaagagtttt ataagttaag taaagatagt gattatatta aaacttctgc
      601 tattgctaag aatattgagt ttcgagcatc aactaaatac ggagagctag agattacgat
      661 aaatttgtca aaacccgaaa aagatccaaa aacaattgct gctgaaaaat tagtaaaagc
      721 cactaattat cccaaatgtt tactatgtat ggaaaatgaa ggatatcagg ggcgaataaa
      781 ttatccagca cgttcgaatc atagaattat tcgattgaaa cttggtgacg aagtatgggg
      841 attccaatat tctccctact tatactttaa tgagcatgcg atatttttaa atagtcaaca
      901 tgtaccaatg gctattacct ctaaaacatt tgaacaatta ttggagattg ttgatatttt
      961 gccaggatat tttgctggtt caaattctga tcttcctatc tcaggaggct ccattcttag
     1021 tcataaccac taccagggag ggaaacatat tttcccaatg gaaaaagcaa aatttgagag
     1081 tgaatttcgt tttaaagact ttgaggatgt taatgctggt attgtaaaat ggccaatgtc
     1141 agtaattagg ttacaaagtg aaaataaaaa tcgcttgtta gatttagcta ctaaaattct
     1201 aaataagtgg agagaatatt cagatttaga agtagacatc attgctatga ctgaagatgt
     1261 cccacaccat acggtgactc ctattgctcg taaagtggac gggagatatg aattagatat
     1321 tgttctaaga gataatcata ctactgaaca gtacccagat ggtgttttcc atcctcatca
     1381 agatgtacaa catattaaaa aggaaaatat cgggttaatc gaagtgatgg gacttgcgat
     1441 attaccacct cgtttgaaac cagagttaga agaagtggga aaatatcttt taggagagga
     1501 aaacgcaatt gcggattatc atttagaatg ggccgaccaa ttgaaagaaa aatatcctcg
     1561 cattaataaa gaagaggtta ataacgtagt tcaacacgaa gcaggacaag tgtttgcgag
     1621 agttctggaa gatgcaggag tttataagaa tacaccgtct gggcatgaag cgtttatgag
     1681 atttgttaag tcggttggaa ttaattagga ggaaacccaa atggcaatat tggtaacagg
     1741 cggagctggt tatattggta gccataccgt agtagaatta ctaaatttag gaaaggaagt
     1801 catcattgtc gacaaccttt cgaactctag catcttggtg ctagaccgta ttgaagcaat
     1861 tacaggaata cgtcccgtgt tttacgaatt agatgtttgt gataaacaag cattgagaaa
     1921 ggtatttgaa caagaatcga ttgatgctgc aattcatttt gcaggttata aagctgtcgg
     1981 agaatccgtg caaaagcctg tgatgtacta caaaaataat attatgagta cattggcact
     2041 tgttgaagtg atgtcagaat ttaatgttaa aaagattgtc ttttcttcaa gtgcgactgt
     2101 atatggaatt aacaatcagt cacctctaat tgagacgatg caaacaagtg cgacaaatcc
     2161 ttatgggtat acgaaagtga tgcttgagca aattttaaaa gatgttcatg tggcagattc
     2221 agaatggagt attgcgttgc ttcgttattt caatccaatt ggtgctcatg agtctggttt
     2281 gattggggaa gatccctcag gaattcctaa caacttgatg ccttatattg cacaagtagc
     2341 ggtaggtaag ctatcagagc taagtgtgtt cggaaatgat tatgatacgc ttgatggtac
     2401 tggtgtgcgt gattatatcc atgtagtaga tttagcaata ggacatataa aagctttaga
     2461 aaaagtatct gaaaaaacag atgtttatat ctataaccta ggctcgggag aaggcactag
     2521 tgtattacaa cttgtaaata catttgaaag tgttaataag atccctattc cttataaaat
     2581 agttccaaga cgttcaggag acgttgcaac ttgttatgca aatgcagaca aagcatataa
     2641 ggaattaaat tggaggacaa caaaatcgat tgaagacatg tgtagagata catggaattg
     2701 gcaatcaaaa aatcccaatg gctataattg atgatactgt ctatatgagt gctgtatttt
     2761 gaaaaaatta cttaaccttt taatgaaatg catatttaat gaaagataaa tttaaaaaaa
     2821 tgtttgcaag tgcttgacaa aggaaacggt ttcattgtat aatgtaaaca tggtgacacc
     2881 gatgtcaaat ataaaaaaga aggtctttat atgaaaaact taacaaaaat taagttcaaa
     2941 gagaatgggg aatttaatca tttccctggg aatacagttg tagcaaatct ttatactaaa
     3001 caagatttga tggaagttgt tgatattatt caatcacgtt atagagaatt accatttatc
     3061 gataagttta ctttaactcc aaggaattct attcacatga ctgtaattga attgttgtgc
     3121 catgaaaatc gtgaaacgga gttttggagc agtaatcttc ctctagatac acctttacag
     3181 gaaatacatg attactttgc taaacaactt gaaatttttc cattgttgga tgaagaaatt
     3241 catatgcgta taactgaaat gggaaaacaa aacatactag ttgaacctgc agatgaagct
     3301 tctgcaaaga gattagaaga aattcgtact tatgtctcag aaaaagcagg tgtttgtttc
     3361 cctaatcatg atagatatca attccatatt tcaattgggt atcttcggat tcctctaacc
     3421 gaagaggagg aagaagagtt tactaaagtc agagcagaat taactgaaat tttattagag
     3481 aagattccaa ctattactgt aaaccgtatt gactatactg tatttgaaga tatgagacaa
     3541 tttgttccat atcacgaaaa atttaaataa aataataaaa aaggagaact tatcatgaaa
     3601 aaaatgaaag tttggtctac tgtacttgca acgggagttg ctcttactac acttgctgct
     3661 tgctctggag gttcaaattc tacgactgct tcttcatctg aagaaaaagc tgataaaagt
     3721 caagaattag ttatctattc gaactcagtc tcaaatggtc gtggtgattg gttaactgct
     3781 aaagcaaaag aagctggttt taatataaaa atggttgata tcgctggcgc tcaattagca
     3841 gaccgtgtta ttgctgagaa gaataatgca gttgcagata tggtatttgg aattggtgct
     3901 gttgattcaa ataaaattag agatcaaaaa ttactagtac agtacaagcc taaatggtta
     3961 gataaaattg atcaatcttt atcagataaa gataattatt ataatcctgt gattgttcaa
     4021 ccattagttt taattggggc gcctgatgta aaagaaatgc ctaaagattg gactgaatta
     4081 ggtagtaagt ataaaggtaa atattcaatt tatggtcttc aaggtggtac aggacgggca
     4141 attctagcaa gtatcttagt tcgttacctt gatgaaaaag gagatttagg cgtatcagaa
     4201 aaaggatggg aagttgcaaa agaatacttt gcaaatgcct atactcttca aaaaggtgaa
     4261 agctcagttg ttaaagcgtt agataaagaa agcccaattc aatacggtat gatctggggt
     4321 tctggagcgt taattggaca aaaagaacaa aatgttgaat tcaaagttat gactcctgag
     4381 attggtgtac catttgtaac tgaacaaact atggttttaa gcactagtaa aaaacaagcg
     4441 ttagctaaag aatttattga ttggtttggt caatcagaaa ttcaagtaga atatagtaaa
     4501 aaatttggat ctattcctgc caataaagat gccctaaaag agttacctga agatacgaaa
     4561 aaatttgttg atcaagtgaa accacaaaat attgactggg aagctgttgg aaagcatttg
     4621 gatgaatggg tagaaaaagc tgaattagaa tacgtaaaat aagaaataag agttattttt
     4681 tatgtccaag tctctctatt atttctaaat ttaaggaaga aataaagaga ggcttttggt
     4741 tttagtaata gagggagtta gtgagatgat taaatttgat aatattcaaa ttaaatatgg
     4801 tgattttgtt gcaattgata atctgaattt agatatacat gaaggggaat tctttacatt
     4861 tcttgggcct tcaggatgtg gtaaatcaac tactttgaga gcattggtag gttttttaga
     4921 tccatcatca ggaagtattg aagttaatgg aacagatgtc actcatttgg aacctgaaaa
     4981 gcgtggaatt ggtattgtat ttcaatctta tgcgctattt ccaactatga ctgtttttga
     5041 taatattgca tttggtttaa aagttaagaa ggtagctcca gatgttatta aagctaaagt
     5101 atcagcagtg gcagcaaaaa ttaagatctc tgatcaacag ttacagcgta atgtatcaga
     5161 attatctggg ggtcaacaac aaagggtagc attggctcgt gctctggttc ttgaacctaa
     5221 aattctttgt ctagatgaac cattgtcaaa ccttgacgca aaattacgtg tagatttgag
     5281 aaaagagttg aaaagacttc aaaaagagtt aggtattact actttatatg ttactcatga
     5341 tcaagaggaa gccttgactt tatctgatag aattgcagtc tttaacaatg gatacatcga
     5401 acaggtcggt acaccagtag agatttatca taattctcaa actgaatttg tatgtgattt
     5461 cattggagat attaatgttt tgaccgatga aacagtccac gaagtattat tgaaaaatac
     5521 aagcgttttc ttagaggata aaaaaggata cattcgatta gagaaagttc gattcaatcg
     5581 tgaaactgaa caagatttta ttctaaaagg gacaattatt gatgttgagt tttctggagt
     5641 tacaattcac tatacaataa aagtttctga aagtcagatt cttaatgtaa caagtattga
     5701 tagtcaggct gctattagat ctgtcggaga aagtgtggaa ttatttatca caccatcaga
     5761 cgttctgcaa ttttaaggag gtgcagtatg cgtcataaat taaatttaaa agattggctt
     5821 attcgtttag ggttaatctg gttcttagta acatttatta tttatccaaa ctttgatcta
     5881 gtagtgaatg tatttgtaaa aggaggagaa ttttcccttg atgctgtaca tcgtgttcta
     5941 aaatctcaga gggcacttca gagtattatg aacagtttta agttagcatt ttcactcatt
     6001 attacagtta atgtcgtagg tattctttgt gttctattta cagagtactt tgatattaaa
     6061 ggtgctaaaa ttttaaaatt aggttatatg acctctttaa tttatggagg agtggtttta
     6121 gcgactggat ataaatttgt ctatggtcct tatggattga ttacaaaatt tttacaaaat
     6181 gttatccctt ctttagactc taactggttt attgggtatg gtgcagtctt attcattatg
     6241 acattttcag gaactgctaa tcatacattg tttttaacaa atacaattcg aagcgttgac
     6301 tatcacacta ttgaggctgc tcgaaatatg ggagcaaaac catttactgt tttccgaaaa
     6361 gtagtgttac caaccttaat tccaactcta tttgcactta ctattatggt ttttcttagt
     6421 ggtttatctg cagtagcagc acccatgatt gttggtggta aagaatttca aactataaat
     6481 ccaatgatta ttacatttgc agggatgggg aattctcgtg atttagctgc cttacttgca
     6541 attattttag gtattgcaac tacaattttg cttactatca tgaataagat agaaaaaggt
     6601 ggaaattata tttctatctc taagactaaa gcgcctctta aaaaacaaaa aattgcgtct
     6661 aagccttgga atatcattgc tcacattgta gcatatggat tgttcacagt tttcatgctt
     6721 ccactaattt ttatagtatt atactcattt acagatccag ttgcaattca aacaggtaac
     6781 ttaacattat caaactttac tttagaaaat tatcgcttat tctttagtaa tagtgcggca
     6841 ttctctccat tcttggtcag ctttatttat tctattattg ctgcgacaac agcaacaatt
     6901 ctcgcagttg tatttgctcg tgttgtcaga aaacataaat ctcgttttga tttcttattt
     6961 gaatatggtg ctctacttcc ttggttacta ccaagtacac ttttagcagt aagtttatta
     7021 tttactttta atcagccaca atttcttgtc ttgaatcaga ttttggtagg tagtttggta
     7081 attctactta ttgcatatat agttgtaaaa atcccatttt cttatagaat ggtacgtgct
     7141 attttattta gtgttgatga tgagatggaa gatgcagcaa gaagtatggg tgcttcacct
     7201 ttttatacta tgatgaaggt tatcattcca tttattttac cggttgttct ctctgttatt
     7261 gctttaaact ttaactcttt attaactgac ttcgacttat ctgtattcct ttaccatccc
     7321 ctagctcaac cattaggtat tacgattcga tctgcaggtg atgaaacagc aacatctaat
     7381 gcacaagctc tggtatttgt ttatacaatt gttctgatga ttatttctgg aacggtatta
     7441 tacttcacac aaagaccggg gcgtaaagta aggaaataat catgacagcc actagtcttg
     7501 ggttatcaaa tattgaaata gttgtcagga ttgttttatc agtagtcatt ggtagtataa
     7561 ttggtttaga gagagggagc aaatcccagc ctgcaggcat ccgaacttat agtattgttt
     7621 gtctagctgc atgtttgatt atgatgacga atgaatacgt atcttataaa tttgggacag
     7681 gagatcctac acgattagga gctcaagtta tatcaggtgt gggttttcta ggcgctggaa
     7741 cgattcttat tacagataaa aagaaaatta caggtctgac aactgcagca ggcatttggg
     7801 cttcggcagg aattggatta gctattggag taggttttta tgagggagct cttttagtag
     7861 ccatttctgt ttggggtgtg atatccatgt tccaaccact aaaaaaatat ctgcaaaatc
     7921 gttctaaaat gattgaattg tatatagtgg ttaaatctac agaagcctat aatcgtgtat
     7981 tggtttattg tgcagaaaat ggtatcagaa tgaccgattc aagaacagcg tttggagatg
     8041 ttaattctga cagaattgaa tactttgatg ttccggacaa aaaaatagcg tcatttatta
     8101 ctctggaatt atcaggtaga tttgaacatc ttcgactgat ggaagaaatt gctaatattg
     8161 taggtgttat ttatgtcgaa gaaattagct gaaatttagt aaacaaaggg acagatgttt
     8221 ttgtcctttt gctttttaga tgttttaatc atctaatttt tgagtagatt tttgctcaga
     8281 ggtttttatg aaagattata aaatcaattt tgatttaggt aaaatagaat attttgataa
     8341 taattgtttg atacaagtat acaaatttat ttctttttat gatatttgtg aaatggtgtt
     8401 tgccttt
//
LOCUS       DAVKWZ010000073         8133 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000073 DAVKWZ010000000
VERSION     DAVKWZ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8133
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.073"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(126..290)
                     /locus_tag="VW854_001868"
     CDS             complement(126..290)
                     /locus_tag="VW854_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEV0263056.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            complement(457..2052)
                     /locus_tag="VW854_001869"
     CDS             complement(457..2052)
                     /locus_tag="VW854_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263057.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPRSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTN
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKVQ"
     gene            complement(2064..2474)
                     /locus_tag="VW854_001870"
     CDS             complement(2064..2474)
                     /locus_tag="VW854_001870"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV0263058.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETTRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            complement(2590..3381)
                     /gene="yghU"
                     /locus_tag="VW854_001871"
     CDS             complement(2590..3381)
                     /gene="yghU"
                     /locus_tag="VW854_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEV0263059.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTEDVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            complement(3394..6090)
                     /locus_tag="VW854_001872"
     CDS             complement(3394..6090)
                     /locus_tag="VW854_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEV0263060.1"
                     /translation="MSKEQKRQAFYTQSPEEVLKAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            complement(6328..7299)
                     /locus_tag="VW854_001873"
     CDS             complement(6328..7299)
                     /locus_tag="VW854_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV0263061.1"
                     /translation="MNRNNPILYIVIPCYNEEQVLPYTNPMFVEKIEQLVKKEEIHPL
                     SKIMYVNDGSKDRTWELIESYANSIPSVVGVSQSRNRGHQSSVPAGMYEAIQFADIVI
                     TTDADGQDDINCMDKMISEYKSGSEIVYGVRDNRDTDSAFKRITAEGFYKVLRLFGAE
                     VVFNHADYRLVSKRFLKELEKFTEVNLFLRGLMPLVGFKYSYVSYKRHERIAGESHYP
                     LSKMLGLAMDGITSLSIKPIRLITSLGVLTSLFSFLLIIWVLLSKFVGTVVTGWASTY
                     AIVSLLGGVQLISLGVIGEYIGKIYLETKRRPRYIISERTFDKDRIL"
     gene            complement(7286..8056)
                     /locus_tag="VW854_001874"
     CDS             complement(7286..8056)
                     /locus_tag="VW854_001874"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263062.1"
                     /translation="MIIAIIMVSLLTQKIEMKPLIAAIIAIFVVSFPMFFAINRINST
                     AGFDLDYEWEMTYHHFIKMGLNSETDGGYLANDVNGSISIENKEERVIYNKQIIAQRI
                     KDYGVTGLINHQAKKTLNNYNDGTFGWGLEGSFFEKIFPNDSYVAKITRSFYYSSGKL
                     HLIFQGIVQSVWLSILFLVLGNVLIGREKMTREFLVLMTSLIGIFLFESIFESRSRYL
                     YTFIPIYIVAATVGFQQMTQNFKKRALKLKTGVKNEQK"
BASE COUNT         2443 a         1806 c         1491 g         2393 t
ORIGIN      
        1 tttttatcgc tctgaacatc aaaaaagaaa ggacgaattt tgtcctttct cgagcttagc
       61 tgacttcaac ccactacagt tgacaaagag cctgaaaaac ctcggtttga ccgaggtttt
      121 tctatttatt tgggccatcc taaccagaca gccacgagaa caagggctgt gccagctaaa
      181 cttgttaaga tagataagaa tttatctttt ttctccttga actgagaaaa acctatcttc
      241 aaagcgagca gtccgagtag cattgaagca accatcaaat aaaaacccat ttctttgtcc
      301 tccattaggc ttaatttacc ttattataac atatatttat taaataaacc ttttttatac
      361 tcaatgaaaa tcaaagagca aactaggaaa ctagccgcag gctgctcaaa acactgtttt
      421 gaggttgcag atagagctag agattttcga agagtattat tgtactttaa aggtcttgag
      481 ataattgtct tttcctactt gaccttcgaa ggttttacca ttttcaaggt aaggaaggtc
      541 atcggatact gaagccttta ccttgtatat cttgccatca actttaaaga agtagacagt
      601 gtctcccttg ataacagctg atttgaggtc tgctactact cccttgatgc tttctgtcgt
      661 tgcattgtca atttcaaggt cgtttttatt ggcatacttg ctgagcatct cttccactgt
      721 agtagcaacg ataacatttt ggtactcgac tgcgtctacc agggcgtact ctttgaccaa
      781 gccagcattg tcctttaagc ccatgatgta gagaggcttg tcattgaggt tgatgaggat
      841 tgggaaggtt gctttgtagg atttctcctg aacagcacct tctgctgatt cacgggctga
      901 ttcttctgtc gcagaagcca agctatactt agtaatttct cctgttcgca tattttcaag
      961 gatgaaacca agattactct catccgcatt agccgacgtc acacctgtgt agagatagat
     1021 gtcattaccg atagacaagt aattatagcc attggtagtc tgggtcacgt ttttcttgga
     1081 aatcatggca ttcaagaaac cgtccttgta cttgccgttg tagttgattt gctcaatggt
     1141 ttcctctgct ggatagatcc tgtccaccca ttctggaaca tctgataagc tgtattcctt
     1201 ggtttctcca tttgtagcat ccaagataat gactgaagca ggacgaggaa ccgcaagtcc
     1261 aaattgcttt tggtaaaccg ttgctacata gaaaggattg ccctcatcgt ccacctcaaa
     1321 agatggagtt ttaaagattt tagtcgggta cttcaagcgc aggtgacgtt tgacatcacg
     1381 gttaaaatac tccgagtctg aatacttgat tggtgtcttc aagtccacca aatccgcatt
     1441 tccagttacc atgtccacct tgatatactc accgattccc ttggcttgat tgttaaacca
     1501 tttgataggg tctgcatatt ctagtggtgt gacccgataa ggtttcccat caattgtcaa
     1561 ttgggtatag gtatctgccg ctacgtattg cgacacctta tcggttaggg aacccaagta
     1621 gcggtctcca attttttcag cagtacttct atctaggata ggaaccttac tggtgtcact
     1681 cctaggaaat tcagtaaagt ctttttccgt aaccgtgact acattggcat aatttttagc
     1741 ctgaaacatg ctggaagtta ccaaggaaac caaagctgcc aagagaagaa ttcctccaat
     1801 cgaagctaac agaattttcc ctaaccgatt gattttgaaa ccctcaagat ttaaggcagc
     1861 ttccgcctta ccgtggcgca catgaaccgt tttgacaagg tttatcccct tgccaaagcc
     1921 aaataagatt gccacaacta acaaatgccc acagaggaag aagagaaatt cccagctggt
     1981 caggttaagg ggcggtaaaa agatatacca ggtcgttgcg ataaaaataa gttcaaagac
     2041 tattcgtttc atttgctttc ctcctaaatg attcgtcctt ccaagtgatc cagctcatgc
     2101 tggcaaatct gagctgggaa gcctgtcaag gtaatggtct gttcctgcca cttgctgtca
     2161 cgataggcaa gccttgtggt ttcataacgc ttagttgatc tcacacctac caaggacaaa
     2221 cagccttctt ctgcctcata ggatccttca aaggacagaa gcactgggtt aaacatgacc
     2281 acgggaacca agccaagatt aaagataatc acgcgcttct gcaccccaat catattggca
     2341 gctagaccaa cacaggtatc acgatttgct aagagtgtat cctgcaaatc tctggcaaga
     2401 taaaggtcct cctgacttgc cggctgagac acttgagata aaaataagat atccttcaca
     2461 attttctttt ccacttcctc acactcctct tgctttttac tatattatag caattatagc
     2521 acaaaagccg ataactgcaa accctttccc tcaactgtag caaaaagcca tccgaagatg
     2581 acttttttgc tatttaattt ctgtataagt tacttccaag ccacgcttaa cagctggacg
     2641 attggcaatt ttttctgccc attttactag attttgataa cttgaggcat ccaagaattt
     2701 tgcagaacct tggtaaagat ttccttgaac taactgtcca taccaagacc agatagcaat
     2761 atctgcaatc gtatagtcat tgcctgcaat ataaggtttc tgagccaatt ccttatccaa
     2821 taaatccaac tggcgtttca cttccattgt aaaacggtta ataggatatt ccaatttttc
     2881 aggagcataa ttgaagaaat gtccaaatcc cccacctaga aaaggtgctg cacctgcttg
     2941 ccagaatagc caattcaaaa cttctacctt ttccacagga ttacttggta aaaaggctcc
     3001 aaatttctca gcaaggtaaa gaagaatatg agcagactca aagactctta catcttcagt
     3061 acctgactgg tccaataagg ctggaatctt ggaatttgga ttgagcttca caaagtctga
     3121 tccgaattga tccccatcca tgatagcaat cttatacaag tcgtaagccg cttccttaaa
     3181 accagcttct agtaattctt ccaataagat agtaaccttc acaccatttg gtgttcccag
     3241 tgaataaagc tgaaaagctt gttctccttt tggcaagttt tgttcgaaac gggcacctgc
     3301 tgttggtctg tttagccccg taaaagctcc ttgattacta gcttcatcct gccatacggt
     3361 cggtaattga tatgctgaca tccgaaacct cccttaaatc gcattcttgt caaaaccgag
     3421 tttgcgttga ataaacttaa cgatttcgac gatgataatc attgagaagc ttccagccat
     3481 aacaattccc cattgtgaca agtctagttt ggttacgtgg aagattcctt caagcggttc
     3541 tacaacgatt gttgccatga gaaggataaa ggataccaag atggaccagt taaaggtctt
     3601 agacttgaat gggccaactg tcaagatgga ttggtagaca gacttgacat tgtaggcatg
     3661 gaagagctga atcaaaccaa gggttgcaaa ggccatcgtt agggcatctg catgaatagc
     3721 atgattgtca cccacatgaa ctgggtaagc aatcgcaagg ccataaacac tcataacaag
     3781 agctgcttgg agtacacctt gataaatgat agaactcaaa acaccacctg agaagaagct
     3841 tgccttgcgt ccacgtggtt tatgattcat gacaccaggc tccgcaggtt caacaccaag
     3901 agcgatagct gggaaggtat ccgttaccaa gttgatccac aaaagatgaa ccggctgcaa
     3961 gacatcccaa ccaaacaagg ttgataggaa gatggttaat acttcagcag tattagcaga
     4021 aagtaggtac tgaatagtct tttgaatgtt tgagaagacc ttacgtcctt cttccactgc
     4081 gacgataata gtcgcaaagt tatcatctgc aagaatcata tcagaagccc ccttagaaac
     4141 ctctgtacca gtgattccca taccgatacc gatatcggct gttttcagag ctggcgcgtc
     4201 attgacaccg tcacctgtca tggcaacgac tttaccttgt ttttgccaag ccttgacgat
     4261 acgaaccttg tgttctggag acacacgggc ataaacagag tattgaccaa cgactttttc
     4321 aaattcttca tctgacagtt cattgagttc agcaccagtt aaaacgtgac cttctgtatc
     4381 gtttgcgtca atgattccca aacgtttggc aatggcttcc gctgtgtctt ggtggtcacc
     4441 tgtaatcata atcggacgga ttcccgcttc cttagccaca cgaacagcct cagcggcttc
     4501 aggacgttca gggtcaatca tcccaatcaa accagtaaaa attaaatcat tttcaagctc
     4561 ttcagaagtg agattttctg gaatactatc gataatctta taagcacctg caaggacacg
     4621 caaggcttga tgagccattt cagaattgtt tgtatgaatg agatttgtaa ccttctcatc
     4681 aatcggagca atatccccag ccttatcacg aagaagacaa cgttttaaga gttggtctgg
     4741 cgcacccttg actgctacaa ggaaacgacc atctggcaat gggtgaactg ttgacatgag
     4801 cttacggtca gagtcaaatg gcaattcagc tacacgagga tatttctcta agaaaccttt
     4861 gacatcatag cccttgtcca aggcatattg gataaaggct gtttcggttg ggtcaccaat
     4921 caagttacct tccacatcga ttttcgtatc attggccaag acaactgaac gaagtagtgg
     4981 catttcaaga cctagttcaa tatcatcagc tgagtcatgt agaaccgcat cgtagaagac
     5041 tttttcgact gtcatcttgt tcatagtcag cgtaccagtc ttatcagaag cgatgatttc
     5101 agttgaacca agtgtttcaa ctgctggcaa cttacgaacg atggaatgtc gtttggccaa
     5161 aacttgagta ccaagagaaa gaacgatggt aacgatagca ggaagtcctt ctggaatggc
     5221 tgcaacggca agggcaacag aagtcaacaa ctcaccaagt ggatttttcc cttgaatgaa
     5281 gacacccact acaaaagtaa caagggcaat gaccaagata gcataggtca agaccttaga
     5341 aaggttgttc aaattttgtt tgagtggtgt atcagtctca tccgcatctt gaagcatacc
     5401 agcaatatga ccaacttcag tgtacatacc tgtattgaca acaacaccca tcccacgacc
     5461 ataggttacg tttgagtttt ggaaggccat gttgacacgg tcaccaatac cagcatctgt
     5521 cgcaagctcg actgacaagt ctttttcgac tggtacagat tcacctgtca aggctgcttc
     5581 ttcaatttta agagagttgg cttctatcaa acgtaggtcc gctggtacca cgtcacctgc
     5641 ttcaagggca acgatatcgc ctggtaccaa ttctttagag tcaatctctg ccatgtgtcc
     5701 atcacgaaga acgcgggcaa ctggactaga catggatttg agggcttcaa tagcttcttc
     5761 agcttttcct tcttggtaaa caccaaaggc agcgttgatg ataaccacag ctaggatgat
     5821 aatggcatct gcgatatctt ccccaccaga agtcacgact gacaagattg ctgccgcaac
     5881 taggatgata atcatcaaat ccttaaattg ctcgataaac ttgaccagga ttgatcgttt
     5941 ctcgccttct tcgagttcat tgtggccaaa ttcggcaagg cgcttttccg cctcacttga
     6001 tgacaaacct tgctcggttg catccacagc cttcaagacc tcttcaggac tttgagtgta
     6061 aaacgcttgg cgtttttgtt cttttgacat gtgtctcctc cttgacattg tgtgcaaaac
     6121 agactctctt tttcccatcg tatcttttca cgacaaacaa aaagagacct gttaatcata
     6181 acaagtctcg ctgtttaaga tagtgccgga aagcatactt ttcagtataa aattcggaat
     6241 gacgacacta tcacaggttt ctgccagcta ctcccttgag tagtactatt ataccaaatt
     6301 ttgaaaagtt ttcaaagagt aaagattcta taaaattcta tccttatcaa aagtacgttc
     6361 actgataata tatctaggac gacgtttcgt ttctaggtaa atctttccaa tatactcacc
     6421 aattactccc aaactaatca attgtactcc accaagtaaa ctaactattg cataggtgct
     6481 tgcccatcca gtcacaaccg taccaacaaa cttactcaat aaaacccaaa taatcaatag
     6541 aaaactaaag agtgacgtca aaacacccag gcttgtaatc aaacgaatcg gtttgattga
     6601 taaactggta ataccatcca tagctaaacc aagcatttta gatagtggat aatggctctc
     6661 accagcaatt cgttcatggc gcttatatga aacgtaagaa tatttaaaac caactaaagg
     6721 cattaaacca cgaagaaata gattaacttc agtgaatttt tctaattcct tcaagaatct
     6781 tttagaaacc aagcgataat cagcatgatt aaaaacaact tctgctccaa ataagcgtag
     6841 cactttataa aaaccttcgg ctgtaattcg tttaaaagca gagtccgtat cacggttatc
     6901 acgtacaccg tatacaattt cagaaccact tttatactca gaaatcatct tatccatgca
     6961 gttaatatca tcctgtccat cggcatcagt ggtaatgaca atatcagcaa actgaatagc
     7021 ttcatacatc cctgcaggga cactactttg atgcccccta ttacgacttt gtgatacacc
     7081 aaccacagaa ggaatggaat tagcataact ttctatcaat tcccaagtcc gatccttact
     7141 gccatcattt acatacatga ttttactaag gggatgtatt tcttctttct ttactaattg
     7201 ttcaattttt tcaacaaaca ttggattagt atatggtagg acttgctctt cgttataaca
     7261 tggaataact atatataaaa ttggattatt tctgttcatt tttcactcct gtttttaatt
     7321 taagagctct ttttttaaaa ttctgggtca tttgttgaaa tcccactgta gctgcaacaa
     7381 tatatatcgg aataaaagta tataaatatc ttgatctgga ttcaaatata gattcaaaca
     7441 gaaaaattcc tattaacgaa gtcattaata ctagaaattc tcttgtcatt ttttctctgc
     7501 ctatcagtac attccctaaa actaaaaata aaatactcaa ccatacactt tgtacgattc
     7561 cttgaaaaat tagatgtagt ttacctgaag aataataaaa acttctagtt atctttgcta
     7621 cataagaatc atttggaaag atcttctcaa aaaaactacc ttctaacccc caaccaaaag
     7681 ttccatcatt atagttatta agagtctttt tagcttggtg gttaatcaaa cctgtaactc
     7741 cgtagtcctt aattctttgt gcaattatct gtttattata gataactcgc tcttctttat
     7801 tttcaatcga tattgaacca ttaacgtcat ttgccaaata tccaccatct gtctccgaat
     7861 ttaaacccat ctttataaaa tggtggtacg tcatttccca ctcataatca aggtcgaatc
     7921 ctgcagttga atttatgcga tttatggcaa agaacatagg aaaactcaca acaaatattg
     7981 cgattatcgc agcgataaga ggcttcattt ctattttttg agtcaagaga ctgaccataa
     8041 taattgcaat aatcattata gcaatttgag gtttcactga ataacctata attccaatag
     8101 cactcagtaa aactgatttt agaatgtaat gca
//
LOCUS       DAVKWZ010000074         8094 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000074 DAVKWZ010000000
VERSION     DAVKWZ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8094
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.074"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            157..456
                     /locus_tag="VW854_001875"
                     /pseudo
     CDS             157..456
                     /locus_tag="VW854_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
     gene            527..2548
                     /locus_tag="VW854_001876"
     CDS             527..2548
                     /locus_tag="VW854_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV0263063.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLSYKALVPVIKGR
                     LPLKRLMALTLLCQLVAVFTVGYAVKTGLTSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDTENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLTDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASLFILSSRDLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            2550..3191
                     /locus_tag="VW854_001877"
     CDS             2550..3191
                     /locus_tag="VW854_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0263064.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNCVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            3295..4647
                     /locus_tag="VW854_001878"
     CDS             3295..4647
                     /locus_tag="VW854_001878"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEV0263065.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQCLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYSNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGLFEEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLVKDLASKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRGVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            4651..5709
                     /locus_tag="VW854_001879"
     CDS             4651..5709
                     /locus_tag="VW854_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEV0263066.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGA
                     VTKVSDQKNPEPDELVDIQTHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLLELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            5719..6918
                     /gene="divIB"
                     /locus_tag="VW854_001880"
     CDS             5719..6918
                     /gene="divIB"
                     /locus_tag="VW854_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEV0263067.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEEPES
                     KEKEEQDKKLAKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVVDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNRNQT
                     TQRSSRR"
BASE COUNT         2513 a         1372 c         1736 g         2473 t
ORIGIN      
        1 tagtaaaaca tctaaaaaat tcgatttttt ccttgacaac aaataataaa aaagttattc
       61 ttatcacata agaaagcgct tttaataaaa gagagaggag gatttatttt gttgcattgt
      121 tgctcaaggt gtgctttttt tgaaagaaaa aataaaatga aaacgaaaaa acatagatta
      181 cttgctctag ctcttatttc aaattttaca ttattgggag ctgcatcagc tgctgtacaa
      241 tatccagatg gaggagtatg gacatatgga gaaggttcag gaggtggtta ggctttttca
      301 aattactatc atggtaaaaa atatcattat tcatccatcg tgagcagatg ggatggtcat
      361 tctgataaag gagaagctcc tgctggaaaa acctcctatg catggatttg gactaaatgg
      421 ggagaacaag tagcatttta ctgtgattat gactaatcgt tcattgattt aaatcgttcc
      481 ccttgtaaga aattacaggg ggaattttgt ttacaaggag tctactatga agaaactatt
      541 catattatta tcaacttttt ttctcagctt cttccttgct tggattattg tcttacgtgc
      601 gccacaatat ttatatgcaa gctatgattc cgtttcttta cttcgtgtca aaaaagatac
      661 tcaggaaccg acgcgtgagg tatttgaaca ggaattggag aattttgcaa actcagaaca
      721 gagtttaata gctagaagaa ttgtagagcc gagtaaggat gggacgactc actttactta
      781 tgcaacttat ggtcagggaa ctttaccaaa agaattccaa gaagctagcc aagaaagtcg
      841 tgaacgtagt gatccgctaa atagttatct ccttttgtca ggctccttga cgaaagaaaa
      901 gcttgccgat aaattaggag atttgggtta taaagcaagt gctgaccgaa agataccgcc
      961 ctattttctt gcttttcgaa tattactaaa tccccttatt ttaattagtt tagcaatatt
     1021 tggcttatct ttctttgctt tagtgattat cactcggatt aaggaaatga gagctgcagg
     1081 tataaaactc ttttctggtc agactctctt atccatcatg gggcattctt tatcgactga
     1141 tatcaagtgg ctccttctat cagccctcct ttccttccta ggtgggggag tcgttctttt
     1201 tagtcaaggt ttgttttatc ctatcttgtt agccacctat ggttttggga ttagtttcta
     1261 tctgttgttt ttattggcga tttcaatttt actaatgctt ctttatctaa tgagtttgag
     1321 ttacaaagca ttagttcccg ttattaaggg aagattaccc cttaaacgct tgatggcttt
     1381 aaccctattg tgtcagttag tagctgtttt tacagtaggc tacgctgtta agacaggttt
     1441 gacgtcttac caacgattga aagaacttga aatttcaaaa caagcatggc aggacagagc
     1501 agactattac cagatttcct ttggcttagg tgatagagga aaagatacag aaaatcagag
     1561 caagtggtat gcctttgcca aggaagcaat cgaagaagaa caagctcttt atgtaaagga
     1621 taatctgctc cattttgcca atccacaagg aaaaaatgaa cagggagaga cactggatac
     1681 ctatagtcca gatgctaata cgctctatgt tagtcccagt tatttggaca aggaaaaggt
     1741 cgtggtagat gctgagacca aacagaagtt agcccatctc caaaaaggtg agtttatcct
     1801 cttgctccca gaacatttgc gctctcgaga agcagaactt aagaaagttt ttgaagaaag
     1861 attgagttat tatggaaaat ctggtgagga ggcaagtgct cctttggatt atgagatgaa
     1921 agcgcacgtt agttatcttt caatgggaga aaagcggttt gtttataata acggtgagaa
     1981 tcccgtatct actcagtatt tgactgatcc gattttagtg gtattcacgc cgacttctac
     2041 aggtgatagt tttatatcct tatctagttg gtctatcaat gctggaaaac aactctttat
     2101 caaaggatat gagagtgggc tagaactctt gaagaaagct ggaatttatg agcaagtatc
     2161 ctatcttaaa gaaggaagaa gtgtttatct aactcgttat aatgaagttc aaactgaaac
     2221 agcaacttta atcttaggag ctattgtggg gatagctagt tccttgttac tattttattc
     2281 tgtcaatctt ctatatttcg agcaattccg ccgagatatc ttgattaaac gaatttcagg
     2341 tttacgattt tttgaaacac atgctcagta tatggttagc caatttgcca gttttgtatt
     2401 tggtgctagt ctctttattt taagcagtcg agacttggtg attggcttgc tcactttatt
     2461 ggtctttcta gctagtgcag ttttgacgct ttaccgtcaa gcgcagaaag aatctcgtgt
     2521 ttctatgaca attatgaaag gaaaatagga tgattgaact aaagaatata tctaaaaaat
     2581 ttggaagccg tcagctattt tcagatacga atcttcattt tgaaggtggg aaaatttatg
     2641 ccttaatcgg tacaagtggc tgtggtaaga caacactctt gaatatgatt ggacgattgg
     2701 agccatatga caaagggcaa atcatctatg atggcacttc tcttaaggac atcaagcctt
     2761 ctgttttctt tagagattac ttgggatact tatttcaaga ttttggctta attgaaagcc
     2821 aaaccgtcaa agagaatctc aatctgggtt tagttggtaa aaagttgaag gaaaaagaga
     2881 aaatctcttt gatgaaacaa gctctaaact gtgtaaacct ctcttatttg gatttgaagc
     2941 aacctatatt tgagttatca ggaggagaag cacaacgtgt tgcactagcg aagataattt
     3001 taaaggatcc gcctttgatt cttgcagatg aaccaaccgc ttccttagac cccaaaaatt
     3061 ctgaggaatt actttccatc ctagaatctt taaaaaatcc gaatcggacc attattattg
     3121 cgacccacaa tcctctgatt tgggagcaag tggatcaggt cattcgagtt accgatttat
     3181 cacatagatg atatggtaag attcagttag aagaaagagt cacaaacaca ctttgtggct
     3241 tttttatttc cataaaaatg gtaaaatagt aggagtagaa atggagttcg agacatgaaa
     3301 gtaatagatc aatttaaaaa taagaaagtt cttgttttag gtttggccaa gtctggtgaa
     3361 tctgcggctc gtttgttgga caagttaggt gccattgtga cagtaaatga tgggaaacca
     3421 ttcgaggaca atccagctgc ccaatgtttg ctggaagaag ggatcaaggt cattacaggt
     3481 ggccatcctt tggaactctt ggatgaagag tttgccctca tggtaaaaaa tccaggtatc
     3541 ccctacagca atcccatgat tgaaaaggct ttggccaagg gaattccagt cttgactgag
     3601 gtggaattgg cttatttgat ttcagaagca ccgattattg gtatcacagg atcgaacggt
     3661 aagacaacca caacgactat gattggggaa gttttgactg ctgctgggca acatggtctt
     3721 ttatcaggga atatcggcta tcctgccagt caggttgctc aaatagcatc agataaggac
     3781 acgcttgtta tggaactttc ttctttccaa ctcatgggtg ttcaagaatt ccatccagag
     3841 attgcggtta ttaccaacct catgccaact catatcgatt atcatggatt atttgaggaa
     3901 tatgtagcag ccaagtggaa tatccagaac aagatgacag cagctgattt ccttgtcttg
     3961 aactttaatc aagacttggt aaaagacttg gcttccaaga cagaagccac tgttgtacca
     4021 ttttcaacac ttgaaaaggt tgatggagct tatctggaag atggtcaact ctacttccgt
     4081 ggtgaagtag tcatggcagc gaatgaaatc ggtgttccag gtagccacaa tgtggaaaat
     4141 gcccttgcga ctattgctgt agccaagctt cgtggtgtgg acaatcaaac catcaaggaa
     4201 actctttcag ccttcggtgg tgtcaaacac cgtctccagt ttgtggatga catcaagggt
     4261 gttaaattct ataacgacag taaatcaact aatatcttgg ctactcaaaa agccttgtca
     4321 ggatttgaca acagcaaggt cgtcttgatt gcaggtggtt tggaccgtgg caatgagttt
     4381 gacgaattgg tgccagatat tactggactc aagaagatgg tcatcctggg tcaatctgca
     4441 gaacgtgtca aacgggcagc agacaaggct ggtgtcgctt atgtggaggc gacagatatt
     4501 gcagatgcga cccgcaaggc ctatgagctt gcgactcaag gagatgtggt tcttcttagt
     4561 cctgccaatg ccagctggga tatgtatgct aactttgaag tacgtggcga cctctttatc
     4621 gacacagtag cggagttaaa agaataaaat atgaaaaaaa ttgtctttac aggtgggggg
     4681 acggttggac acgttaccct caatcttttg ttaatgccca agttcatcga agatggttgg
     4741 gaagtccact atatcgggga caagcgtggt atcgaacacc aagaaatcct taagtcaggt
     4801 ttggatgtca ctttccactc cattgcgact gggaaattgc gtcgctattt ctcttggcaa
     4861 aatatgctgg acgtcttcaa agttggctgg ggaatcgtcc aatcgctctt tatcatgttg
     4921 cgacttcgtc cacagaccct tttttcaaag gggggctttg tctcagtacc gcctgttatc
     4981 gcagcgcgtg tgtcaggagt gcctgtcttt attcacgaat ctgacctgtc tatgggcttg
     5041 gccaataaaa tcgcctataa atttgcgact aagatgtatt caacctttga gcaagcttcg
     5101 agtttgtcta aggttgagca tgtgggagca gtgaccaagg tttcagatca aaaaaatcca
     5161 gaaccagatg aattggtgga tattcaaacc cactttaatc ataaattgcc gactgtattg
     5221 tttgttggcg gttctgcagg tgctcgtgtc tttaaccaat tggtgacaga ccataagaaa
     5281 gaactaacag agcgctacaa tattatcaat ctaactggag attctagcct gaatgagttg
     5341 agccaaaatc tttttcgtgt tgactatgtg accgatctct atcaaccctt gttggaattg
     5401 gctgatattg ttgtgacacg aggtggtgcc aatacgattt ttgagctctt ggcgatagca
     5461 aaattgcatg tcattgtgcc gcttggtcgt gaagctagtc gtggtgacca gattgaaaat
     5521 gcagcttact ttgtaaaaaa aggctatgca gaagaccttc aagaaagcga tttgaccttg
     5581 gatagtttgg aagagaagct ttctcactta ctaagtcaca aggaagatta ccaagctaag
     5641 atgaaagctt ctaaggaatt gaaatctcta gcagattttt atcaattgtt gaaaaaagat
     5701 ttatcataag gaaagtaaat gtcaaaagat aagaaaaatg aggacaaaga aatcctcgaa
     5761 gaattgaaag agttatcaga atggcagaaa cgaaaccaag aatatctaaa aaagaaggct
     5821 gaagaagaag cggctctagc tgaggagaag gaaaaggaaa gacaagctcg gatgggagaa
     5881 gaatctgaga agtcagagga caaacaggac caggagagtg aaacagacca ggaagattca
     5941 gaatcagcta aggaagagtc tgaagaaaaa gtagcatcct cagaggctga caaagagaaa
     6001 gaagagaaag aagaaccaga gtctaaagag aaggaggaac aggataaaaa gcttgctaaa
     6061 aaggctacaa aggaaaaacc agccaaagca aagattcctg gtatccatat cttgcgagcc
     6121 ttcacgattt tatttccaag tctgctttta ttgattgtct ctgcctactt gctcagtcct
     6181 tatgcgacca tgaaagatat tcgtgttgag ggaacggtgc aaactacagc tgatgatatt
     6241 cgacaggctt caggcattca ggattcggat tatacgatta accttctgct agacaaggca
     6301 aaatatgaaa agcagattaa gtctaactat tgggttgaat cagctcaact tgtctatcaa
     6361 tttccaacta agttcactat taaggtcaag gaatatgata ttgtggccta ctatatttct
     6421 ggtgaaaatc attatcctat tctttccagt ggtcagcttg agactagttc tgtgagtctg
     6481 aacagtttac cagaaactta tttatcagtt ctctttaatg atagtgaaca aatcaaggtt
     6541 tttgtctcag aacttgctca aattagccca gaactcaagg cggctatcca aaaggtggaa
     6601 ttagccccaa gcaaggtgac atccgattta attcgattga ccatgaatga ttcggacgaa
     6661 gtcttggttc ctctatctga aatgagtaag aaactgccat attacagtaa gattaagcct
     6721 caattgtcag aaccgagtgt ggtcgacatg gaagctggaa tctacagcta cactgtggcg
     6781 gataaattaa ttatggaagc tgaggaaaaa gccaaacaag aggccaagga agctgagaaa
     6841 aaacaagaag aagaacagaa aaaacaagag gaagagagca atcgaaacca aacgacccag
     6901 cgttcatcgc gtcgctaggt ttaccttttc tcttatagtt ctttagtgac catgttttta
     6961 cgtttaatat ttgacatctg tttttcttta tgttacatct gcaatgtaat cgattacaaa
     7021 ataatttttg atgaagaagg taacacatat gtcagatgaa gtattcttat ttgaagaaat
     7081 tgaagaaatc gtagccccta cagatggtga atttttgggg gaagttttac ttggaactgg
     7141 ggtagttctc ttaattggag tagcctgttg ttaaaaagat agggagtgat aatcatgcaa
     7201 gataactttt tatttgagga aatttcagta ccagttagtg atttttcagc tggacttgca
     7261 acaggtatcg gatttggttt agcaatcctt gctcttgctg gttgttgaag tttgttcatt
     7321 tactaacatc aagctttttc aatttcattt tagacagtca tttaaatttt ccgtattagt
     7381 cttgcagcaa gagattaata gaattagtca ttattttatt gattgcggac tgagggacta
     7441 gagtatgttt tacttaaccc ctcttttatt tattaaaggt taggtttgtt atgagaattg
     7501 ttgataagat taagatatta cctactcctt atgagggaca ctatcattta tatataccat
     7561 ccagtaagaa acatgtatta gttgggaaac aggaaaaaaa tggttagagc aactaatagg
     7621 tcaagaattt accatatcgg acttattagt gttagtaggg aagaaatatt tttaaaatat
     7681 cttgggactt taatataaca ttatctgaaa aattaaacta taaaagattt aataagaatt
     7741 ttgaaaaaat cctatttggt attttcccat tggctcttat tttgcaatgt tttattatgt
     7801 atatttatag ggcggatatt ttgactaatt tccaaaactt tccaataacg ctaaacattc
     7861 ttttattttg gttgcttgtt gttattatat ctgcaacgat acatgaattt agtcatgcaa
     7921 taattgctaa taattatcaa attgagatcc caaattgtgg aataatgcta cttttgttta
     7981 atttggcttt ttttgtagat ttaagtgggg tgcaattttt aaaaaataaa aaacaatttt
     8041 tgaaaattat gttagcagga attgcttcaa attcgatttt atcacttaca ggtt
//
LOCUS       DAVKWZ010000075         7875 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000075 DAVKWZ010000000
VERSION     DAVKWZ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7875
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.075"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(254..550)
                     /locus_tag="VW854_001881"
     CDS             complement(254..550)
                     /locus_tag="VW854_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="HEV0263068.1"
                     /translation="MKSKRMEFHNKFLIVSAMLAVISWLSLGVVSFPMLAGTLGISTK
                     AAATVVNLISAYSTVTAVISIVGAITGVGSIGSGIAATVLYILKKKGAAKAALW"
     gene            complement(1081..1974)
                     /locus_tag="VW854_001882"
     CDS             complement(1081..1974)
                     /locus_tag="VW854_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0263069.1"
                     /translation="MDELKREIGYKIKTIRRSCGKTQIDICGDESELTIRQLARIENG
                     QALATLPKLLLIADKLGVSLQNIVDVKVIEIPKGFLKLKDELIHSQTYADKGRIERKE
                     AILEEIYENYYENLPEEEQLIVDVTQARFDIYGSSDVTYGLGLVEEYFQQLLKKKYFS
                     VNDLLIIELYFFCCAMGLEDKEHFEELAQKVLLCSEYEDKASLVQMEKVLLSLFIQIQ
                     TEDSLIYIQTFEKIIAKTRHVFYRPHLFLLKAKYALFVDKNILEAESFYEKAISLAEL
                     LDDQVLVQRILAEKQIDFPTT"
     gene            complement(2728..2958)
                     /gene="blpU"
                     /locus_tag="VW854_001883"
     CDS             complement(2728..2958)
                     /gene="blpU"
                     /locus_tag="VW854_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HEV0263070.1"
                     /translation="MNTKKMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGVARGLQ
                     LGIKTRTWQGAATGAAGGAILGGVAYAATCWW"
     gene            complement(3161..3451)
                     /locus_tag="VW854_001884"
                     /pseudo
     CDS             complement(3161..3451)
                     /locus_tag="VW854_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(3497..3730)
                     /locus_tag="VW854_001885"
     CDS             complement(3497..3730)
                     /locus_tag="VW854_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV0263071.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQSVGDVVKIIQGK"
     gene            complement(3745..4728)
                     /gene="plsX"
                     /locus_tag="VW854_001886"
     CDS             complement(3745..4728)
                     /gene="plsX"
                     /locus_tag="VW854_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEV0263072.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIVGLLKTAITGGGLRAKLGALLLKDSLSGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREF"
     gene            complement(4725..5495)
                     /gene="recO"
                     /locus_tag="VW854_001887"
     CDS             complement(4725..5495)
                     /gene="recO"
                     /locus_tag="VW854_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEV0263073.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            complement(5492..6661)
                     /locus_tag="VW854_001888"
     CDS             complement(5492..6661)
                     /locus_tag="VW854_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV0263074.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQAISEIPGVLRLTLGEPDFTT
                     PDHVKEAGKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPAAFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            6810..7820
                     /locus_tag="VW854_001889"
     CDS             6810..7820
                     /locus_tag="VW854_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEV0263075.1"
                     /translation="MSFLSKNGAGILACLLISILSWYLGGFFPVVGAPVFAIFMGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKELSKFLIVMAMSAIGLKTNLVAMVKSSGKSILLGAICWIAIILTTLGMQTLIG
                     IF"
BASE COUNT         2299 a         1826 c         1245 g         2505 t
ORIGIN      
        1 gaaaaatata gtttttcctt atatagaaag tatttatttt tttactttgg ataggcagtt
       61 tcatattata acgattcaaa gtagcgtaat ctactaccaa aaaaagtaaa aataatttcc
      121 cttgaaaagg aagatagctt ttaaaataga ttgacaaact tatgataaca aaaaacaaaa
      181 gggttatccc aataaaatta agataaccta aaatactttc acattctttt ttagaaagta
      241 ttttcatcta gttctaccat agagctgctt tagcagcacc tttctttttc aatatataaa
      301 gaactgttgc tgcaataccc gaaccaatag aacctactcc tgtgattgca cctacaattg
      361 aaattactgc tgtaactgta ctataagcac taatcaaatt gactacagta gcagcagctt
      421 ttgtcgaaat tcctaatgtc ccagccaaca taggaaaaga aacaacacct aagcttaacc
      481 aactaatgac tgctaacatt gctgaaacaa taagaaattt attatgaaat tccattcttt
      541 tagatttcat tttagtgtac tccttttgta tattatgtat gttttattga gagttaattc
      601 gcacatgttg tattcctaac tctcttcaaa tgttcgtcta tattttatac tacttttatt
      661 aacatatcta taataaataa attactacta tattttccta gattatcaaa ttacttttat
      721 atcactaaga ctcaataaaa gataatgtct atttaacttt tatacgatta ctaccttctt
      781 tttagtttac acttcctaca tcataaataa acatactcaa ccctcacaat ctccaaataa
      841 ctcttctata tcattgaaaa ttgagtttca tcctctatac taatgtagga cattccttta
      901 atccaatata agaatacctt gttccattaa tctctacccg atattataga tagaaccaaa
      961 ttcctatact taaaaggaca aataacgttc caaccataat aaacctcctt tctttttgag
     1021 aacgttttct ttataggttt agtatagcaa aaacatgaaa aaaacaacat gacttagaag
     1081 tcaggttgtt gggaaatcta tctgtttttc tgctaaaatt ctctgaacta atacctgatc
     1141 gtctagcaat tctgctagag aaatcgcctt ttcatagaaa ctttctgcct ctaaaatatt
     1201 cttgtctaca aataaagcat attttgcctt tagcaaaaat agatgaggtc ggtaaaaaac
     1261 atgtcttgtt ttagcaataa ttttctcaaa tgtttgaata tatatcagac tatcttctgt
     1321 ctgaatttgg ataaataggc tcagaagaac tttttccatt tgaactaggc tagccttatc
     1381 ttcatattct gaacatagca atactttttg agctaactcc tcaaaatgtt ctttatcttc
     1441 taatcccata gcacaacaaa agaaatacaa ttcaataatc aacaaatcat ttacagaaaa
     1501 atatttcttt tttaataact gctggaaata ttcttccact aaccctaaac cgtaggtcac
     1561 atctgaacta ccataaatat caaaacgagc ttgtgtcaca tccacaatta actgctcttc
     1621 ctcaggcaaa ttctcataat agttttcata tatttcctca agtatagctt cttttcgttc
     1681 gattcttccc ttatcagcgt aggtttgaga atgaatcagc tcatctttga gttttaggaa
     1741 tccttttgga atctctataa ccttaacatc cacaatattc tgtaatgata ctccaagttt
     1801 atccgcaatc aataataatt taggaagtgt tgctaaagct tgcccatttt caatacgtgc
     1861 tagctgacga atcgtcaact ctgattcatc accacaaata tctatctgag tttttccaca
     1921 agatcgccta atagttttga ttttataacc aatttcacgt ttcaactcat ccatagtaat
     1981 actccttaag ttgattacaa agatttcttt ttcttcctaa ttcttttgtc aatcatatat
     2041 aaaacgataa gcgaaaacag cggtagaaca atataactct tttcactcat tatcagctga
     2101 aatcccaggg ttgctagtat cttaaagatg aaaatattca tcaaaaatag aaaaacaaat
     2161 actaaaaaat attttaaaac cttcttcatt tcttttcttc aactttccta atttctacac
     2221 ctattttatc gaaaatatat ataaaactcc aaataaacac ttcgactgca ctttttttgg
     2281 taaaaaatag acagaacaaa catcctacct actaaaaata tgatatgaaa ggaaaatgat
     2341 tttttactga caccctaggc aagcttggga caaataaatc attgatagca acatttacga
     2401 catcctattt aaagttcctt caatttctaa tgtccccgct atttctaagc cacctctggt
     2461 atgatctatt cctagtcaga atagataaca ccgttctaaa tcacgattca tagttactcc
     2521 ttttttgact cgttactata agactcctct ttacatgcgc aaaataattt tgattcagat
     2581 tcatcattta tagttttgat tattttttaa cagatttttc tgttttcttc tccgaagact
     2641 ttaaaaattt ttttctaatt aaatcatcca tataaggacc aaatatacca actactaaac
     2701 caataataaa acttttaaaa tccataatta ccaccaacat gtcgctgcat aggccacacc
     2761 tccaagtata gctcctcccg cagcaccagt tgctgcacct tgccatgttc ttgttttaat
     2821 tcctagctga agacctcgtg ctactcctcc tccaacacct gctttggcaa aatctcccca
     2881 attgcatccg ccaccttcaa cgcaagcaag catctcagta tccataattt caaattgtga
     2941 catctttttt gtattcataa cgaatacctc ttttttattt ttaatatttg tcttgttaca
     3001 aacttgacaa gtttagtata acagtatcta ttaatttttt tcatccaaat cttgaattgg
     3061 catcgaaacg tcttgaatta gcttttttgt ttcaaaatca tctctatttt taaaaaagat
     3121 gttttctaat cactttttta ctattagact tcctgcaaaa ctagaatcct agttcatgat
     3181 tgataatacc agcaatcaaa ttcattcgta atccgaagcg tttacgatga tttcgatagg
     3241 ttgttgaaaa cattttaaac gtttttactt tggcaaagat attctcaacc ttgcttctct
     3301 ccttagatag cgcatggtta caggctttat cttcaactgt tagcggcttg agtttgctgg
     3361 atttacgtgg agtttgtgct tgaggatata tcttcatgag cccttgataa ccactgtcag
     3421 ccaagatttt accagcttgt ccgatatttc tcatttctaa aaaccatcta cttccgttga
     3481 cttggaactc cgattgctat tttccttgaa tgattttaac cacatctcct acactttgga
     3541 gttggtcaat ttcctcatcg ctgatttcga tactaaattc atcttccaga gtcaagataa
     3601 actccatcaa gtcaacagaa tccgcatcca aatcgtcttt cagactcaag gattctgtca
     3661 cgacaaagtc ctctccctgt cgctcttgga taatggtcac aatacggtca aaaatttctt
     3721 tttctgtcat ctcttttatt ctcctcaaaa ttcacgcgca gtctgggcaa ccacgtctgt
     3781 ttctagcatg gtacgaatct gacgaatcgt actataaaca gccttggcat cgcttgagcc
     3841 atgagtcttg acaacaggtg ccttaacacc aaacaagacc gctccaccaa catctgaata
     3901 attgagctgt tttttcaaac cactgaggct gtccttgaga aggagggcac ctagtttcgc
     3961 tcgaagacca ccacctgtaa tagctgtctt gagcaagccc acgattccca tagctgtccc
     4021 ttcgatggat ttgagcacag cgtttcccgt gaaaccatct gccacaacaa catctgcaac
     4081 gccattcatc aaatcacgcg cttccacgtt tccgataaag ttcaaacttt catcagccgc
     4141 cagtaattca taagtttcct tacgaagcgg gtcgcccttg ctactctctg ttccgttgtt
     4201 gagcaaacca acgcgtggtt gcgcaatgcc acggacattt ttagcataga aggaacctag
     4261 aaccgcatat tgatggaggt gctgggctgt attttctgca ttggcaccaa ggtctagcat
     4321 gtcaaaacct tttccatcaa cggtaggcaa ggtagacatg agtccaggac ggtcgatatt
     4381 cttgatacga cccacgatga agaatcctgc tgccaacaag gcacctgtat tcccagccga
     4441 aaggacagcg tctgcttcac catctttgac agccttggct gccaatacca tactggcatt
     4501 tttcttattc ctaatagctc tcgtaggttc atcatccgaa tcaatcttct catccgtatg
     4561 gataatgctg acgcgctctg tcgctgtcag atattgcttg attttagctt catctccgta
     4621 aagttgaacc tcgatatctg aaaagtcaga tagggcttga ttgacaccct caacaatggc
     4681 ctgaggtgcg taatcgcccc ccatggcatc tactgcgatt tttttcattt cttttcctct
     4741 tttagtaatt gtccccagtc tgctagggaa tcaataaatt tctttgattt taggtgaatc
     4801 ccaacgtact cttcatataa ttgatccata aattggcgta gctcttgctt gattccaggc
     4861 ttgagcgaaa tggtctccaa agtctcaaaa tcaatagctt gaaattgatt gagcagatag
     4921 gggatattgg gattgagatg acaacgtctc ttatcctcat gataatgctc tggacagagg
     4981 caggctccat atttgaaaga aaagtcaaaa gcctgaccaa cccgatggca gaagacacac
     5041 tcattaaaat tgaggctgat tccaaatcga gtcaaaattt gaatttcaaa aatattggtc
     5101 aaaacctgat aatccaagcc tgcttccatc aactccaaag tcttttgcaa aaaagcaaac
     5161 aagggagcat cctgctgatt gtcctgcaaa ctagcatctg caagagctgc cacataggtc
     5221 gcataggcca tgacaaagag gtcactatta atcttgggaa aagtcatgac ctcatgatag
     5281 tcttcgatgt aactgagtcc gtcatcattg attcgcaaga gaaatcgtgc cagcaccaag
     5341 ggctgaataa caggcgccag cttagactga ccagcgtgtt tgacaaaaaa catgcgtttg
     5401 ccaacctgct ctgtaaaaat tttgacgagc ttgtcatcct cacgaaaatt gcgattgtaa
     5461 agcaccaagc cttgactcgt gatagactga atcatgcttc tctcatgtac tcctcaagtc
     5521 gtttcatggc ttctttgata gtctccatgc tggctgcata agataggcgg acgtagcctt
     5581 ccccgtaacg tccaaaggct gcaccaggga taaaggcaac ggccttcttc tgagcaaaat
     5641 ccttcagaaa agcaaaggag tcttgattgt agcccgctgg aattttagca aaaatataga
     5701 aggcaccgtc tggtttgata atctcaaaac caagagcagt cattttttcg atgatatagt
     5761 cccgacgttg gatatattcc ttcttcatgg gctccgcatc gtttttacca gccgtcaagg
     5821 cttctaccgc agcatgttgc gccatggtat ttgcggcagt gaccaagtac tggtgactct
     5881 tgattaactg ggctgtgaag gccgcaggag cgaaaatcag ccccaaacgc caacctgtca
     5941 tggcatgcga tttagacaaa ccattgataa taatagcctg gtctctcaac atcgttccta
     6001 gagacacatg ggcttcgcct gtgtaggtca attctgagta aacctcatca cagacaacaa
     6061 aaatttcgta cttgcgtaaa acagctgcca aggcctctaa ctgctctcga ctgtaggtaa
     6121 ttcctgtcgg attggctgga tagttgagaa taaccgcctt gagcttatca ccctgctcca
     6181 aaatggcctt ctccaacatc tcaggagtca agacaaaacc attttcagtc gtatcaatct
     6241 caacaatttc tgccccaact aagttaacaa tcggttcata gcctggataa gcaggagctg
     6301 gcaaaagtac cttgtctccc tcttccaaaa tagccgtcaa agtcgcagat aaagcctctg
     6361 tcgccccaat tgtaaccaag atttcatttt caggagcata gtccagttgg tacttttcct
     6421 taacaaagtc actggctgcc tgacgtagag tcagcagacc actcatccct gtatagtagg
     6481 attggttctg atcaatcgct cgcttgcccg cctccttgac atggtctggc gttgtaaaat
     6541 caggttcccc caaggtcaaa cgcaagaccc caggaatctc cgaaatagcc tggtcaaact
     6601 gacgaatcaa cgaaacttga attttatcta actgtttatt aaagcgctta gttaagtcca
     6661 tagatacctc ctactttcaa aacgtatctc tattatacta caaaagcccc ccgaccgcaa
     6721 aaatacatta gagaatagct ttccactttc tattttactt ttcctgttta taggtctata
     6781 atggtaatag attttatttg gaggttttta tgtcatttct atcaaaaaat ggagcaggta
     6841 tcttggcctg ccttctcatt tccatcctat cttggtactt aggaggattc ttccctgtgg
     6901 ttggcgcgcc cgtttttgcg atttttatgg gcatgctcct acatcccttt ctctcgtcct
     6961 ataaacaact ggatgctggt ttgaccttta gttccaagaa gttgctccaa tatgccgttg
     7021 tcttgcttgg ttttggtctc aatatctcgc aggtcttcgc agttggccaa tcttcactcc
     7081 ctgtcatcct gtccactatc tcaatagctc tgattattgc ctacctcttc cagcgtttct
     7141 ttgcactgga tacaaaactg gctaccttgg ttggagtagg ttcttctatc tgtgggggtt
     7201 ctgccattgc agcgacagcg cctgttatcc atgctaagga aaaggaagta gcccaagcca
     7261 tttccgttat ctttttcttc aatgtcttgg ctgcgctcat ctttccaacc ctcggcacct
     7321 ggcttcatct atccaatgaa ggcttcgccc tctttgcagg gactgcggtc aacgacactt
     7381 cctctgtaac ggctgccgcc agcgcttggg acagtcttta ccaaagcaat accctcgagt
     7441 ctgcaaccat tgttaaactc acacgtactt tggccattat ccctatcacg ctctttctat
     7501 cctactggca aagtcgccaa caagaaaaca agcaaagcct acaactgaaa aaagtcttcc
     7561 cactttttat cctttacttt atccttgcct ctctcctcac tacactactc acctctctag
     7621 gtgtgtccag tagtttcttt acccctctca aagaactctc taaattcctt attgtcatgg
     7681 ccatgagtgc tatcggtctc aaaaccaatc tggtcgctat ggtcaaatcc agtggaaaat
     7741 ccattcttct tggagccatc tgctggattg ccatcatcct caccactctt ggtatgcaga
     7801 cccttatcgg cattttctaa tactcttcga aaatctcttc aaaccacgtc aacgtcgcct
     7861 tgccgtatat atgtt
//
LOCUS       DAVKWZ010000076         7432 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000076 DAVKWZ010000000
VERSION     DAVKWZ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7432
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.076"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(76..1164)
                     /locus_tag="VW854_001890"
     CDS             complement(76..1164)
                     /locus_tag="VW854_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEV0263076.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            complement(1182..1850)
                     /locus_tag="VW854_001891"
     CDS             complement(1182..1850)
                     /locus_tag="VW854_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEV0263077.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVTQN
                     SRSI"
     gene            complement(1869..4307)
                     /locus_tag="VW854_001892"
     CDS             complement(1869..4307)
                     /locus_tag="VW854_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEV0263078.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMSIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            complement(4458..5756)
                     /locus_tag="VW854_001893"
     CDS             complement(4458..5756)
                     /locus_tag="VW854_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV0263079.1"
                     /translation="MAKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASGNLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVVTQLLVLVISTVVYFPFFKIQDRLAYQNEIKQS"
     gene            complement(5773..6081)
                     /locus_tag="VW854_001894"
     CDS             complement(5773..6081)
                     /locus_tag="VW854_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV0263080.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQASGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            complement(6127..6447)
                     /locus_tag="VW854_001895"
     CDS             complement(6127..6447)
                     /locus_tag="VW854_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV0263081.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            complement(6642..>7432)
                     /locus_tag="VW854_001896"
     CDS             complement(6642..>7432)
                     /locus_tag="VW854_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HEV0263082.1"
                     /translation="ENYVKEKYSLESLEIIPNEFDDTPTILSERISQVAAGVLRNLID
                     DNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYEMSRKFH
                     GECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGKHQWLDM
                     LTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQSLAVAYG
                     DTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
BASE COUNT         2433 a         1536 c         1061 g         2402 t
ORIGIN      
        1 tatttcaatc ttctatagaa ttcaatacag ggatcaataa aagactacca tttggaaaac
       61 agtagtcctt attgtttatt ttgaatttac ataggcatct acagcgataa tagcttgagc
      121 aacatctgaa ggcgaaatct taaacggcat ctgatgaatt gtctcaccct ccatagttgc
      181 ttgtttacca acttttatta aatcatcata tccaacttga tccaaatgca tttcttttag
      241 agttgttggc ataccaattt ttttgtaaaa ctcaatatac ttatcaagtt cttctttagg
      301 tctattttcc aataatagtt gtactaaagt tccataagct actttttcac catgtgttaa
      361 atgatgaatg tcacctgtca atgcagtaaa accattatga attgcatgcg ccgcagctaa
      421 tcctccactt tcaaaaccta gaccactcaa taaagtatta gcttcaacaa tattttctaa
      481 tgctggtgtc accactttag cttcacaagc tgccatagcc tgtaaaccat ctgcaaacag
      541 cgtttcttca catttcttcg caattgcaac tccagccaat gtttgctgtt gtcccaacat
      601 agtttttcca tttgcctgca taaccgcacg cgcctcaacc caagttgcta aaccatctgc
      661 aataccagac gctaatagac gcttaggggc ttgtgaaata acttttgtat caaccaaaac
      721 taaatctgga tttttagaat aaaatagata atgatcaaat gcaccttcat ctgtataaat
      781 aacagataaa gcagatacag gtgcgtcggt cgatgcaatt gttggagcaa taataacagg
      841 cttttcaatc aaatctgcaa tagcttttgc gctatcaatc gtctttcccc caccaagacc
      901 gataatacta tcacaatttt ctttctcagc caaggcaaca actcgattga tttcattgtc
      961 agaagcttca ccattaaata gcgccagaac aatatggaaa ccatacctat gtagataatc
     1021 ttcaaatcgt tttccaacaa tatcataaac caactgatcg cataatagaa tagggtaatt
     1081 tcccaaatcc aaaattgatt tggcattttc aaacaaggca ttttccccct gaatatatct
     1141 agaaggacta gcaaaaattc tcatatgtat ttcctctcta tctaaatgga acgactattt
     1201 tgagtaacaa accaatcgtc agaaaaatca tcaaccgcct tttggataga tggcatggcg
     1261 aaagctgatt caaaaacatc tgctcctgct gtaacagcat gcgcacctgc agctaaagca
     1321 ttatttactt gtgctacatt tttaaaggat gcagctaaaa tcttactagg agagttctgt
     1381 ctatcaatag caagagctaa ttgacgaatg acagaatttg aatcaatgtt cagattttcc
     1441 attctattat aatatggagc taggtaatcc gctcctgctt cgatagctaa taatccctga
     1501 ataactgtat aaatagctgt tgcagtgata tggtagccct ctttttttag cacctttatt
     1561 gcacgtaatc cagctggagt aacaggtact ttgataaata tatcatctcc tgcttgtctt
     1621 cgaatttcat gagcatcctt taagatgcct tcaaaatctt gagaaatcac ctgaacatga
     1681 atagagggtg tagagccaat caattctctt acatctttga ttcgttcaaa aaaattaata
     1741 gaaccctctc tttttgcaat agtgggattt gaagttaccc cagctagcgg caaaatttca
     1801 gaccactttt taatctcatc taaatttaat gtgtcaagca taaattccat aaaaagaacc
     1861 tctttatttt acaaagtatg ctcagtacgt cctataatgt catcttgggt tgccttagaa
     1921 agaacattga agaatgcaga gtatcctgca acacgaacaa ttaagtctct gtgtttttca
     1981 ggatgtttct gagcgtcaat caacgtctct ctggaaacaa cattgtattg aatatggtac
     2041 ccatgtaaac gattaaagaa tgttcgtaac aaagcaatta gttttaattt atcttcttct
     2101 ttggctaacg tttgaggatt tactttctga tttaagagaa ccccacctac gatttcatct
     2161 gttggtaatt ttgaaacaga ttttaaaaca gatgtagggc cgtgttgatc catattatgt
     2221 gatggtgaac aaccctctgc taacggtgta cccgcgttgc gtccatctgg agttgctaat
     2281 gttccacgtc cctgccctac gttggctgat atagaagatg ttcctgaata acgaattcct
     2341 ccaataggcc ctcttccata acgtgtatta ggatatttag caatttcatc aacataaatg
     2401 tcataagcag cagtaaccaa tttgtcagca taatcatcat cattaccata cttaggtgca
     2461 tcatgaatca acatttcttg aatgacctta ccttcttctc cggcataatc tgtttccagt
     2521 gcatgccaaa gctgacttgg gcttatacgt tcttcctcaa acaccaattt tttaattgca
     2581 gctaatgaat ccgacaaatt tgcaattcca acttgcaatc ctgatatata atcatatact
     2641 gctccacctt ctttaaggtg ttttccacga ccaatacaat catcagtcaa tgctgaacat
     2701 agaatatcag gaacttctcg ttccaatgat aaatcaatag aattttcaac aataacactc
     2761 attcgtgtca aatatcttag tgttttatcc caagcatttt ctaattcaga aaagttcttc
     2821 atatccttaa aatgaccaaa gcttggtgca aaccgtttac ccgaagccgg atcaattcca
     2881 tcattcatcg tgataagtag aaccttaggg aagttcatat aactcatacc tgtgcaacga
     2941 tagccccatt tccctggaac tgccgtttca acacatccaa tggcactgta atcataagca
     3001 tcatcttcca atactccttt tgcaataaaa gaaggaataa taatctcatc attattaaat
     3061 gcaggcatac caaaaccaag tttcatcact tcaatacact cattcatgaa acgagcatct
     3121 aaacctgcat ggtaacgtac agttagatta ggttgcggta gatgggtttg tgcaactgat
     3181 tttaatacca aataagataa tgggttaaca gcatccttct tatctcgagt ctgtccacca
     3241 attgtaacat tttgatataa aggacttcct gctgaagaaa atgtatgtga ttgactgcga
     3301 accttattga ttgtaattgt cttaatccaa agatttgtca gacgttcaac aatgctatct
     3361 tctgtttctt taccactttc taaatcagcc ttcatatatg gatacatata ttgatcaaaa
     3421 cggccatatg aaagagagtg gccattagat tcaatttgta aaatacattg aataaaccaa
     3481 actgattgaa tagcttctgc aaaagtagtt gccggttcat atgggactct agagcaaata
     3541 tctgcaatct caagtaattc tttcttacgt ttaggatttg cattttcggc taaactttta
     3601 gcaagagcaa caaagcgctt tgcatatact ttaatagcat cgattacgat aaatatagag
     3661 tcgtaaaaat gatatttatc aatacttgct ggatctgtta aatctagagc tacttttgct
     3721 ttacgagccc gctcttcaaa acctcttaaa ccaaattgca aaagtttctg atagttaact
     3781 gctaagtgag catctccaga attcatctta ccttccatac cgaagaatcc tgtttccata
     3841 taaacagaca cttcttcagg taataaggca ccagctctag cacgtaaatt attattttcc
     3901 caaaacggag caatacttct aagttgttct tttgtttctt ctgtaatata gaaaacatct
     3961 ccatcacgct tttcaaaaag atccaactca ttgagaacaa attctagcgt atattccgga
     4021 aaaataggag catctttatt ggaagaagct tgatttcccg caatcataga ttcttcttca
     4081 atatagatag acatattttc caaaatttct ttcagcatat atgcacgttt taggacatta
     4141 ggttgttcct tgtatcgttc ataggcgcgt gttgctagaa ctgctctctc agcatcaata
     4201 taaggttttt tatttaaaac atcttctcga tatttgttca tcctttcagt aaggctgcca
     4261 aaatattctg tcttgattgt tgttcttgct acttctgtat ttaccatcgt aagcaccttt
     4321 ctttcgaaaa tatttttatt tctttcgaaa atataatacc gatttttata gaaattttca
     4381 agcttttaac tagaaaaatt ttaactctct aatttttagc acaaatgtgt gagtttaaca
     4441 gtaacacaca aatacctcta agattgtttg atttcatttt ggtaagctaa acgatcttgt
     4501 atcttaaaga atggaaagta aaccactgta gatatcacca atactaataa ctgagtcaca
     4561 acaccttgcc atccacccac caaaaatcct gatagaatag ctggtgtact ccaaggcaat
     4621 gttacccctg agaatggctg catgaaacct gttgcaatag ctccatatac tatcacagct
     4681 gcaagtacag gaacaagaat gaaaggtaca aacataactg gattcatgac aatcggaaat
     4741 ccaaatacaa ctggctcatt tacgttaaat attgctggaa aagctgcaac ttttcctaag
     4801 gcttggtatt gttttgattt tgctgcaaaa agcatggcaa ctacaagacc aaacgtaatc
     4861 cctgaacctg atagaattaa aaatgaatct aaaaattgtt gagtaacaat atgtgcacca
     4921 ttttctaatg atagattacc agaggctaac atagctttat tagcatcaag attagataaa
     4981 agcagagctg tcactactcc atttactacc gattgcccat gaacaccaaa ccaccacaaa
     5041 aatgatataa agaatgcaat tccaatagca ccatacaaag atccagttaa accttgcaac
     5101 ggaacttgaa tagcagaata aatcatttct atgaatgttc cgccattagt caatgacttc
     5161 gctaaaatat atacaatcat agaagataag aaaattacaa atgctggaat cattgcttca
     5221 aactgtttgg caatagcttg tggaacttgt tctggcatct taataacaat ttttctcttt
     5281 ataaagaagg tataaatact tcctactacc aaacctataa tgatagcacc gataattcct
     5341 tggcctccaa accaaacttt actaatagcg tccccaatcg cctcaccttg tttagggata
     5401 taagatgatc ttagcaaaat aaagaatgca gatacagata gaactccagc tggtaaagcc
     5461 tctactccgc tattcttagc ataagaatag gcaattgaaa aacaagaaat tagacccata
     5521 atagcaaaag ttcctgaata tacttgcata aacggctctg tccaattagc tccaaaaaca
     5581 ctagcaatgc tcttatttaa tccttcgaac ggcaattgtc ccataatcaa gaacaaacta
     5641 ccaactactg tcaatggcaa aattgctaac atcccatctt ttagagctat aatgccacgc
     5701 atattcacaa acttcatcat cggtgcaatg attttctgaa catccatctt tgccataata
     5761 aatctccttt tcttacccac taatcaaaga tagggccaaa tctaatactt ttttcccatc
     5821 taacatacca tagtccatca tcggaataac agctatcgga acatcacact tatcacaaat
     5881 ttcttttgat ttatctaatg tataagcaac ttgtggaccc aatagtgcaa catctatatt
     5941 tggcgcataa tccgctaatt tagactgaga aaacgcctct atttctgcct caactccact
     6001 agcttgcgct gcaattttca tattatttac aagcatacca gtagaaaaac ctgctgcaca
     6061 aaacaaacca atcttcacca ttatgttttc ctcctctatg ttaataacaa tgataatact
     6121 ctagtattat tttttatgaa gttcttttct caaactaata atttctttga ttaaattaat
     6181 ctccgtcata ctagtcatga gatgatcttg tgaatgaaca aagagagctg taatttctgt
     6241 ctttgtacca gacgcttcct gtgccaagaa ttttgtttgt agattatgag cttctaataa
     6301 ggctgcatca gctaactgga tttcttcttc acatcgttca catgtaccat tttttatgta
     6361 atctaacgct ttataaatat gttgtttcgc atcaccagca tataaaatta aacccatgat
     6421 aatttgatct ggaacaataa tttccataaa ttcctcctca tttcactatg taaaaagctt
     6481 ttttcatttg aaattttaaa attttctttt gtaactatag aatacaacta cataataaag
     6541 ttgtcaatcc aaaaacaaca gataaactat ttttgtttat ataagcacaa atgtgcaatc
     6601 aactaaaaag aaaaacgata aactgaatca gtctatcatt ttcactcacc tagaatctct
     6661 ctaaaagtca aatccccatc ttcttccaaa acttttaaaa ttgtattttt gtctgtaatc
     6721 aaatgattta ctaaattagc acgcaagaca gaaagaatcg aagatacttt cgtatcaccg
     6781 taagcaacag ctaaactttg aggaatattt tttaaatctt ccaaagagat ggctatcgtt
     6841 ctttcctgta agttttcata gacttcttta ccttttgaat caaaaaatcg acaacaaatt
     6901 tctcctacag ttttaacttt ggtcaattct ttaaagtcat cctctgtaag catgtctaac
     6961 cattgatgtt tccctttatt gctaaaatca ccaattccga ctacagctat atctaaatct
     7021 ttccaactat ttttcaaatt ttcaaaatat cttgattgca aaataccatc tgctaacaat
     7081 ttattttctt gcacaatcgt tgcattcata aatgtacact ctccatgaaa ttttctagac
     7141 atttcataaa tcagtgtatt cacatggtat ttagcgtgta tgtgactagg accacctgct
     7201 agaggataga agtgaacatt tcggacactt ttactgtgaa ttaaatctac taaattactt
     7261 aaacttttcc cccaagaaaa gccaattttc atattatcat caattagatt cctaaggacg
     7321 cctgctgcaa cttgagaaat tctttcagat aaaattgttg gagtatcatc aaattcattt
     7381 ggaataattt ctaaactttc caaactgtat ttttctttta cataattttc ca
//
LOCUS       DAVKWZ010000077         7263 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000077 DAVKWZ010000000
VERSION     DAVKWZ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7263
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.077"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(<1..169)
                     /locus_tag="VW854_001897"
     CDS             complement(<1..169)
                     /locus_tag="VW854_001897"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263083.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDGTDVVSSKHCFEVVDGTDVVS
                     SKHCFEVVDGTD"
     gene            402..6832
                     /locus_tag="VW854_001898"
                     /pseudo
     CDS             402..6832
                     /locus_tag="VW854_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750034.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
BASE COUNT         2832 a         1009 c         1388 g         2034 t
ORIGIN      
        1 cgtcagttcc atctacaacc tcaaaacagt gttttgagct gactacgtca gttccatcca
       61 caacctcaaa acagtgtttt gagctgacta cgtcagttcc atctacaacc tcaaaacagt
      121 gttttgagca acctgcggct agtttcctag tttgctcttt gattttcatt gagtactaat
      181 cttacttttt aacgtgtgct aaaaaataaa ttaatgcatg ttaatttttc ttgaattaaa
      241 tttttttaaa tgatatactg tttttataga gagataacaa ttatatataa atgtactact
      301 ctatttccta gataatactt agtaaaactt tttataacaa aggctagcaa agttaaagtt
      361 tattatctat tggaagttaa ataaatatgt aaggaattaa aatgaaaaaa agtacagtat
      421 tgtcattaac cacagctgca gttattttag cagcctatgc ccctaatgag gtagtcttag
      481 cagacacatc tagctctgaa gatgctttaa acatctctga taaagaaaaa gtagtattag
      541 ataaggaaac agaaaataaa gagaaacatg aagatattca taatgctata gaaacttcac
      601 aggattttaa agagaagaaa acagcagtca ttgagggaaa agaagttgtt agtaaaaatc
      661 ctgtgacaga caataacact agcaatgaag aagcaacaat caaagaagat tccaatcaat
      721 cccaaggaga tcatacggac tcatttgtga ataaaaacac agaaaatccc aaaaaagaag
      781 ataaacttgt ctatattgct gaatttaaag ataaagaatc tggagaaaaa gcaatcaagc
      841 aactatccaa tcttaagaat acaaaagttt tatatactta tgatagattt ttaacggtag
      901 tgccatagaa acaactccag ataacttgga caaaattaaa caaatagaag gtattacatc
      961 ggttgaaagg gcacaaaaag ttcaacccat gatgaatcat gccagaaagg aaattggagt
     1021 tgaggaagct attgattacc taaagtctat caatgctccg tttgggaaaa attttgatgg
     1081 tagaggtatg gtcatttcaa atatcgatac tggaacagat tatagacata aggctatgag
     1141 aatcgatgat gatgccaaag cctcaatgag atttaaaaaa gaagacttaa aaggaactga
     1201 taaaaattat tggttgagtg ataaaatccc tcatgcgttc aattattata atggtggcaa
     1261 aatcactgta gaaaaatatg atgatggaag ggattatttt gacccacatg ggatgcatat
     1321 tgcagggatt cttgctggaa atgatactga acaagatatc aaaaacttta acggcataga
     1381 tggaattgca cctaatgcac aaattttctc ttacaaaatg tattctgacg caggatctgg
     1441 gtttgcgggt gatgaaacaa tgtttcatgc tattgaagat tctatcaaac acaacgttga
     1501 tgttgtttcg gtatcatctg gttttacagg aacaggtctt gtaggtgaga aatattggca
     1561 agctattagg gcattaagaa aagcaggcat tccaatggtt gtcgctacgg gtaactatgc
     1621 gacttctgct tcaagttctt catgggattt agtagccaat aatcatctga aaatgaccga
     1681 cactggaaat gtaacacgaa ctgcagcaca tgaggatgcg atagcggtcg cttctgctaa
     1741 aaatcaaaca gttgagtttg ataaagttaa cataggtgga gaaagtttta aatacagaaa
     1801 tataggggcc tttttcgata agaataaaat cacaacaaat gaagatggaa caaaagctcc
     1861 tagtaaatta aaatttgtat atataggcaa ggggcaagac caagatttga taggtttgga
     1921 tcttaggggc aaaattgcag taatggatag aatttataca aaggatttaa aaaatgcttt
     1981 taaaaaagct atggataagg gtgcacgcgc cattatggtt gtaaatactg taaattacta
     2041 caatagagat aattggacag agcttccagc tatgggatat gaagcggatg aaggtactaa
     2101 aagtcaagtg ttttcaattt caggagatga tggtgtaaag ctatggaaca tgattaatcc
     2161 taataaaaaa actgaagtca aaagaaataa taaagaagat tttaaagata aattggagca
     2221 atactatcca attgatatgg aaagttttaa ttccaacaaa ccgaatgtag gtgacgaaaa
     2281 agagattgac tttaagtttg cacctgacac agacaaagaa ctctataaag aagatatcat
     2341 cgttccagca ggatccacat cttgggggcc aagaatagat ttacttttaa aacccgatgt
     2401 ttcagcacct ggtaaaaata ttaaatctac gcttaatgtt attaatggca aatcaactta
     2461 tggctatatg tcaggaacta gtatggcgac tccaatcgtg gcagcttcta ctgttttgat
     2521 tagaccgaaa ttaaaggaaa tgcttgaaag acctgtattg aaaaatctta agggagatga
     2581 caaaatagat cttacaagtc ttacaaaaat tgccctacaa aatactgctc gacctatgat
     2641 ggatgcaact tcttggaaag aaaaaagtca atactttgca tcacctagac aacagggagc
     2701 aggcctaatt aatgtggcca atgctttgag aaatgaagtt gtagcaactt tcaaaaacac
     2761 tgattctaaa ggtttggtaa actcatatgg ttccatttct cttaaagaaa taaaaggtga
     2821 taaaaaatac tttacaatca agcttcacaa tacatcaaac agacctttga cttttaaagt
     2881 ttcagcatca gcgataacta cagattctct aactgacaga ttaaaacttg atgaaacata
     2941 taaagatgaa aaatctccag atggtaagca aattgttcca gaaattcacc cagaaaaagt
     3001 caaaggagca aatatcacat ttgagcatga tactttcact ataggcgcaa attctagctt
     3061 tgatttgaat gcggttataa atgttggaga ggccagaaac aaaaataaat ttgtagaatc
     3121 atttattcat tttgagtcag tggaagaaat ggaagctcta aactccaacg ggaagaaaat
     3181 aaacttccaa ccttctttgt cgatgcctct aatgggattt gctgggaatt ggaaccacga
     3241 accaatcctt gataaatggg cttgggaaga agggtcaaga tcaaaaacac tgggaggtta
     3301 tgatgatgat ggtaaaccga aaattccagg aaccttaaat aagggaattg gtggagaaca
     3361 tggtatagat aaatttaatc cagcaggagt tatacaaaat agaaaagata aaaatacaac
     3421 atccctggat caaaatccag aattatttgc tttcaataac gaagggatca acgctccatc
     3481 atcaagtggt tctaagattg ctaacattta tcctttagat tcaaatggaa atcctcaaga
     3541 tgctcaactt gaaagaggat taacaccttc tccacttgta ttaagaagtg cagaagaagg
     3601 attgatttca atagtaaata caaataaaga gggagaaaat caaagagact taaaagtcat
     3661 ttcgagagaa cactttatta gaggaatttt aaattctaaa agcaatgatg caaagggaat
     3721 caaatcatct aaactaaaag tttggggtga cttgaagtgg gatggactca tctataatcc
     3781 tagaggtaga gaagaaaatg caccagaaag taaggataat caagatcctg ctactaagat
     3841 aagaggtcaa tttgaaccga ttgcggaagg tcaatatttc tataaattta aatatagatt
     3901 aactaaagat tacccatggc aggtttccta tattcctgta aaaattgata acaccgcccc
     3961 taagattgtt tcggttgatt tttcaaatcc tgaaaaaatt aagttgatta caaaggatac
     4021 ttatcataag gtaaaagatc agtataagaa tgaaacgcta tttgcgagag atcaaaaaga
     4081 acatcctgaa aaatttgacg agattgcgaa cgaagtttgg tatgctggcg ccgctcttgt
     4141 taatgaagat ggagaggttg aaaaaaatct tgaagtaact tacgcaggtg agggtcaagg
     4201 aagaaataga aaacttgata aagacggaaa taccatttat gaaattaaag gtgcaggaga
     4261 tttaagagga aaaatcattg aagtcattgc attagatggt tctagcaatt tcacaaagat
     4321 tcatagaatt aaatttgctg atcatgctga tgaaaagggg atgatttctt attacctagt
     4381 agatcctgat caagattcat caaaatatca aaagcttggc gagattgcag aatctaaatt
     4441 taaaaattta ggaaatggaa aagagggtag tctaaaaaaa gatacaactg aggtagaaca
     4501 tcatcatcaa gaaaatgaag agtctattaa agaaaaatct agttttacta ttgatagaaa
     4561 tatttcaaca attagagact ttgaaaataa agacttaaag aaactcatta aaaagaaatt
     4621 tagagaagtt gaggactttg taactggtgg aaaaagaaca gtagaacttg attataaata
     4681 tgacgataaa ggaaatataa ctgcctatga tgatggtagt gccttacaat atgaaactga
     4741 aaaacttgac gaaataaaat caaaaattta tggtgttcta agcccatcta aagatggaca
     4801 ctttgaaatt cttggaaaga taagcaatgt ttctaaaaat gccaaggtat attatggaaa
     4861 tagctataaa tcgatagaaa tcaaagcgac taagtatgac tcacattcaa aaacgatgac
     4921 atttgattta tacgctaata ttaatgatat tgtggatgga ttagcttttg ctggagatat
     4981 gagattattt gttaaagatg atgatcgaat aaaagctgaa actaaaatta gaatgcctga
     5041 aaaaaataag gaaactaaag cagaatatcc ctatgtatca agttatggga atgtaatcga
     5101 attaggagaa ggagatcttt caaagaacaa accagacaat ttaactaaaa tggaatctgg
     5161 taaaatctat tctgattcag aaaaacaaca atatctgtta aaggataaca tcattctaag
     5221 aaaaggctat gcactaaaag tgactaccta taatcctgga aaaacggata tgttagaagg
     5281 aaatggagtc tatagcaagg aagatatagc aaaaatacaa aaggccaatc ctaatctaag
     5341 agtcctttca gaaacaacaa tttatgctga tagtagaaat gttgaagatg gaagaagtac
     5401 ccaagctgta ttaatgtcgg ctttggacgg ctttaacatt ataaggtatc aagtgtttac
     5461 atttaaaatg aacgataaag gggaagctat cgataaagac ggaaatcttg tgacagattc
     5521 ttctaaactt gtattatttg gtaaggatga taaagaatac actggagagg ataagtccaa
     5581 tgtagaagct ataaaagaag atggctccat gttatttatt gataccaaac cagtaaacct
     5641 ttcaatggat aagaactact ttaatccatc taaatctaat aaaatttatg tacgaaatcc
     5701 agaattttat ttaagaggta agatttctga taagggtggt tttaactggg aattgagagt
     5761 taatgaatcg gttgtagata attatttaat ctacggagat ttacacattg ataacactag
     5821 agattttaat attaagttga atgttaaaga cggtgacatc atggactggg gaatgaaaga
     5881 ctataaagca aacggatttc cagataaggt aacagatatg gatggaaatg tttatcttca
     5941 aactggctat agcaatttga atgctaaagc agttggagtc cactatcagt ttttatatga
     6001 taatgttaaa cccgaagtaa acattgatcc taagggaaat actagtatcg aatatgctga
     6061 tggaaaatct gtagtcttta acatcaatga taaaagaaat aatggattcg atggtgagat
     6121 tcaagaacaa catatttata taaatggaaa agaatataca tcatttgatg atattaaaca
     6181 aataacagac aagacactaa acattaagat tgttgtaaaa gattttgcaa gaaatacaac
     6241 cgtaaaagaa ttcattttaa ataaagatac gggagaggta agtgaattaa aacctcatag
     6301 ggtgactgtg accattcaaa atggaaaaga aatgagttca acgatagtgt cggaagaaga
     6361 ttttatttta cctgtttata agggtgaatt agaaaaagga taccaatttg atggttggga
     6421 aatttctggt ttcgaaggta aaaaagacgc tggctatgtt attaatctat caaaagatac
     6481 ctttataaaa cctgtattca agaaaataga ggagaaaaag gaggaagaaa ataaacctac
     6541 ttttgatgta tcgaaaaaga aagataaccc acaagtaaac catagtcaat taaatgaaag
     6601 tcacagaaaa gaggatttac aaagagaaga tcattcacaa aaatctgatt caactaagga
     6661 tgttacagct acagttcttg ataaaaacaa tatcagtagt aaatcaacta ctaacaatcc
     6721 taataagttg ccaaaaactg gaacagcaag cggagcccag acactattag ctgccggaat
     6781 aatgtttata gtaggaattt ttcttggatt gaagaaaaaa aatcaagatt aagataaaag
     6841 ctatagaaaa aaatggttta tgtactgaga ttagatagtg aggtgatgac atagttttgt
     6901 gaaaacagcc atttataact caattattta gtttacttca ctattgatac tatttggagt
     6961 tattaatgaa cttagtttag ataactaatg aattgattga aagggttagt attgacaata
     7021 ttggtcatat tgattagaaa ataaagtcta tcaaaattta aaggctaata gaggtgatga
     7081 gacaatttca aggaattaag tagcgtttgg ttgcttaatt cctttttatt tggaaggaag
     7141 tttttcgtaa ccgcccaata acgaagtcta ttgggcggtt acgatatgat cactgacttc
     7201 gtcagtctta tctacaacct caaaacagtg ttttgagcaa cctgcggcta gcttcctagc
     7261 tta
//
LOCUS       DAVKWZ010000078         6968 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000078 DAVKWZ010000000
VERSION     DAVKWZ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6968
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.078"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(104..379)
                     /locus_tag="VW854_001899"
     CDS             complement(104..379)
                     /locus_tag="VW854_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEV0263084.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(485..1324)
                     /locus_tag="VW854_001900"
     CDS             complement(485..1324)
                     /locus_tag="VW854_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV0263085.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRISRVTGLLSSLLNIRAVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1466..2314
                     /locus_tag="VW854_001901"
     CDS             1466..2314
                     /locus_tag="VW854_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV0263086.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSGWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(2407..3324)
                     /locus_tag="VW854_001902"
     CDS             complement(2407..3324)
                     /locus_tag="VW854_001902"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEV0263087.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            3762..3967
                     /locus_tag="VW854_001903"
                     /pseudo
     CDS             3762..3967
                     /locus_tag="VW854_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(4278..4571)
                     /gene="rpmA"
                     /locus_tag="VW854_001904"
     CDS             complement(4278..4571)
                     /gene="rpmA"
                     /locus_tag="VW854_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEV0263088.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(4588..4932)
                     /locus_tag="VW854_001905"
     CDS             complement(4588..4932)
                     /locus_tag="VW854_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEV0263089.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENY
                     SEFVQTRVITEN"
     gene            complement(4948..5262)
                     /gene="rplU"
                     /locus_tag="VW854_001906"
     CDS             complement(4948..5262)
                     /gene="rplU"
                     /locus_tag="VW854_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEV0263090.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(5298..5385)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(5444..6148)
                     /locus_tag="VW854_001907"
     CDS             complement(5444..6148)
                     /locus_tag="VW854_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEV0263091.1"
                     /translation="MGGKMRLLPIRKISRQSKRLALFLTFCAGYVDAYTFIVRGNTLV
                     AGQTGNVVFLSVELIKNNVSDVRDKVLTLLAFMMGVFLLTIYKEKLRIVKKPILSLIP
                     LAILSIIIAFVPQTVDNIYLVPPLAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLA
                     FGDYFRTKHTPFLREGFIFVSLLSSFVLGVVFSAYLTIFYHEKTILGVPIMMSVFYLS
                     MLFASWQKKVKEKASF"
     gene            6550..6849
                     /locus_tag="VW854_001908"
     CDS             6550..6849
                     /locus_tag="VW854_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEV0263092.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT         2191 a         1412 c         1164 g         2201 t
ORIGIN      
        1 cggaacattc aactttttaa ctctatttat tactagattt cataattaaa aaacctactg
       61 accaagctag aaagcttgat acaataggct ttttaaagac tgattattta acagcgtctt
      121 taagagcttt accagctttg aatgctggta ctttagaagc tgcaattgtc atttctttac
      181 cagtttgtgg gttgcgacct ttacgttctg cgcgctcacg aacttcaaag ttaccaaaac
      241 cgatcaattg aactttttca ccagctgcaa gatagtcagc tactgctgca aatacagctt
      301 caactgctgc tgctgagtct ttcttagtca attctgtagc ttctgctact ttagcgatca
      361 aatcttgttt gtttgccatg ttaatgattc ctccaaataa tttctaatta acaaatataa
      421 tcatatccta aaatcccttt taggtcaagt taaaaacgct atttcttccc attttattta
      481 ttttttagga gtggtaacgt atcaaaatag cccaagcgtt ctcacccgtg tgagtttgaa
      541 taatggaacc cgtttccaaa acagaaattg gcttttcaac ataagcttgt aagctttctt
      601 tcatctcttt tgcccaatca tcactaccag aatatgaaat tccaatctct gctacagcac
      661 gttcagaaag cgatgttatc aactcatcta accatttttt aaatgtttta gttccacgac
      721 ctttaaccat tggctgcaat tcatggtctt tcatttgcat gacagcacgg atattgagaa
      781 gagagctcaa caagccagtt acacggctaa ttcgtccacc tttgacaaga ttttccaaag
      841 ttgaaacacc aatataaagc tctgtatggt ttttaacctc ttctacatga gataaaattg
      901 cctccatatc tttaccttct tgagctaact tcgcagcctc aacaacttgg aatttcaggg
      961 cttggtcagt gaaggaacta tcaacaacag tcacatctgc agtagatagg ctagcacctt
     1021 ggcgtgctgc ttctaccgta cccgaaagag catgggacat atgaatagca agaatctggc
     1081 caccatcttt gcataggtct tcaaaaatct cagcaaagac acctacaggt ggctgacttg
     1141 ttttcggaag attcttactt tcttgcatca actgaagaaa tttaccttct tctttcaaat
     1201 ccgcatcaga ataaacaaca ttatcaatca ttacagataa tggaacaatt gtaatatcta
     1261 attgctttac tagttcaggt tcaatagtaa cagatgaatc ggttacaatc ttaatttttg
     1321 tcatagtatc aatctttcta ttttaggatt cagattggtt tccttacttc taattatatc
     1381 aaaaaaaaga ttaaaaatcc taatggagtc aatcaaattt tccgtaaaat ttgatataat
     1441 caacttataa gaaaagaggt gtcctatgat taaaaaaatt taccctattt ttaccatttt
     1501 actaggtgct gctatttatg cttttggact gacttatttt gtagttcccc atcatctctt
     1561 tgaaggaggg gcgacaggca ttaccctcat caccttttat ctttttaaaa tccctgtttc
     1621 cctcatgaac ctgctgatta atattcccct tttcatccta gcttggaaga tttttggagc
     1681 caaatccctc tattctagtt tactaggaac cttagctttg tccggctggt tagctttttt
     1741 tgagcatatt ccccttcata ttgatcttca aggtgattta ctaatcacag cccttatagc
     1801 gggaatccta ttgggaattg gccttggaat tatttttaat gctggaggta caactggcgg
     1861 aactgatatt ctagctcgta ttctcaacaa atacactcat atatccatag gaaaactgct
     1921 ctttatctta gatttttgta ttctcatgtt gattctccta atcttcaagg atttgagatt
     1981 ggtttcctac acgcttttgt ttgattttat tgtttctcgt gttattgatt tgattggtga
     2041 aggaggatat gccggcaaag gctttatgat tatcacaaaa cgtcctgacc aacttgctaa
     2101 ggcgattaat gatgacctcg gaagaggtgt tacttttatt tctggtcaag gctactatag
     2161 taaagaaaat ttgaaaatca tctactgtat tgtcggaaga aatgaaattg tgaaaacgaa
     2221 ggaaatgatt catcgaatcg atcctcaagc ctttataact attacagaag cccatgaaat
     2281 cctaggagaa ggcttcacct ttgaaaaaga ataaaaagag gtaatgtcgt gacctcaaaa
     2341 gttagactaa atcatctatc ttttgggtta cagacaacct cttttttatt ttatttactc
     2401 aagctcttaa gaccaattcc gagttacttc ttcatcagcc tttaactgat ccactaattg
     2461 gtcaactgag tcaaatttgg tcatatctcg aatgcgatca agccaataaa ccatgacggt
     2521 ttccccataa atatcttgat taaaatcaaa aatattgact tcaaaacgtg cttcttctcc
     2581 atcaaaggtc acatttttcc cgacactagc catagcacga tacttctgtc tttgaatctc
     2641 aacatcaaca acataaacgc catctgctgg catataagta cggtctaaaa gcactaaatt
     2701 cgctgtcgga taaccaattg tacgaccacg agcattacca tgaaccacca tacctcttga
     2761 tggaagcggt gcccccaaaa gttttcctgc ttctttcaca tttccatcta aaatagcttg
     2821 acggatacga gttgaactaa tctttccttt ctcatcttct acaggtggaa caatgataac
     2881 ttctccatca aagtaatcct ttaaatcttc tgctgttttt ttgtcagaac caaatgtata
     2941 atcaaaacct gcaacaataa ttttggcatt catagccttg atataagttg caaagaattc
     3001 ttgtgcagtg agactagcga attgactact aaaatcaagg agatataatt cttctacacc
     3061 ttcgcgcttt aattttcttt cacgttcagc agggttcaaa atatgcaaaa acaaatctgg
     3121 atgataaggc tctaaagcga tctttggaga ttcattaaag gtcataacga cgataggcaa
     3181 caaatccttt ctcgcagcct tgttggcaac acgaaataat tcttgatgcc ccttatgtat
     3241 gccatcaaaa tagccgagaa caacgactga atcagatggt gtgccaatat ctttttggtt
     3301 ttttatagga atagtaataa tcataaaata attatatcat agcgatagct atttctggaa
     3361 cagaaaatct gaaatgttgt ttttttcaca tgaagtgtac ctgttttcaa aaagcacttt
     3421 attctatcgt tgcttaacta tgaactttgc aatattcttc tcaaaaactt gtaggacatc
     3481 ttcaaaattt tgcaaggagt gattagactt gttcggtaac cataaagtgt catactatgc
     3541 ttatgtatga aaaagcaatg caactaactc ctgagaactt taaattacta attggtgccg
     3601 aaaaggtaga atttagaatc gaggtacacc tatggctgta aaatttacaa aatgagacaa
     3661 cttgggcaag atgtttgaag aatttcctaa actccctgat ttgaagcaag tcactttccc
     3721 taatgacaaa gaaaaaagcc aaaacagcaa agaaaaacta gatgactgct ttccaacaac
     3781 tcccatctag tgtgcttcag actgggctat ttttctctcc atctgttagc ttggattctc
     3841 agaccgtttc agctaaagaa tatcttttcc cttatcagaa ggaacggctc aagccattca
     3901 gacaagtgaa gggacgacaa gccaatattt gaaaccagat agcagttctt atagtcaatt
     3961 gaaataaaat ctgaagaaat cgagtaggaa actcatatca atgtttaaca gtgttctatt
     4021 ccagattcat actcaatgaa aattaaagtg caaactagga agttagccgc aggctgtact
     4081 tgagtacggc aaggtgaaat tgacatggtt tgaatttgat tttcgaagag tatcaatcca
     4141 ctatacatat taaaaagtat gggtaccaaa acaaaaaaac aggctctccg aaaactcgga
     4201 aagccttatt ttaagctgtt tctagcttcc tcacctttac atttcaaggt tcgggataaa
     4261 aggttcaatg gaccttttta tttagcgatt gggtaaacag aaacttgttt tttatcgcgt
     4321 cctttacgtt caaagcgtac tacgccttca actttagcga acaaagtatc gtctccacca
     4381 cgaccaacgt ttacacctgg atagatgtgt gtaccacgtt gacggtaaag gattgatcca
     4441 cctgttacag tttgtccgtc agctgcttta gctccaagac gttttgcttg tgaatcacgt
     4501 ccgtttgatg tagaacctcc accttttttg tgggcgaaaa gttgcaagtt gttaagagtc
     4561 atttttaaca taatgttttc ctccgtgtta gttttctgtg ataactctgg tttggacgaa
     4621 ctcagaatag ttctccgata agtttgccat acctaagaaa aatgattcaa agaataactg
     4681 ggtcatttct ctctggtgtg aaggaagatc ttttggtatt tcaaccatca gatagccacc
     4741 ttcatcttcg tttaattcta ggattggttc atagcctgca aatttctcaa tagaattgat
     4801 aaagttaatg gcaagcgtag aaaccgatgc acacacgaca tctaagccgt attcgccact
     4861 ctcggcgtgt ccagtaattt ccgcactcct cagctcgcca tcttcggctc tctcaaagac
     4921 tgcctgtatc atgtgttctc cttaaaatta agcgttgatt gcgttgatga caacttttgt
     4981 atatggttga cggtgacctt gtttacggtg gctacctttt ttaggtttgt acttgtaagt
     5041 aaccactttc ttttgttttc cttgtttttc aacagttcca actacagtag ctccagcaac
     5101 aagtggagtt ccgacaacag tgttttcacc accaacaaga acaacttcgt taaaagtaac
     5161 ttcttgacca gcttcaacgt tcaatttttc aacgtaaact gcttgaccaa cttcaacttt
     5221 aacttgtttt ccgccagttt tgataattgc gtatgtgctc attatgcacc tcctatgatt
     5281 tttatgggtt tccccgattt tttgtgaaga ctcgcctagc atcgtgggac gaaccactta
     5341 aacttgaata atcaagcaac gatgttcgtg cggttgcaca ggatcgtgca tagtcaactc
     5401 ttcaagtata gcatatctcc tattttctta caagtaataa cacctaaaat gaagcttttt
     5461 cttttacttt tttctgccaa gaggcaaaaa gcatgctgag gtaaaaaacg ctcatcataa
     5521 taggaacacc aagaatggtc ttttcatgat agaaaatcgt caaataggct gaaaagacaa
     5581 cgccaaggac aaaactacta agcaggctaa caaatatgaa tccttcacgc aaaaaaggag
     5641 tgtgcttggt tcggaaataa tctccaaaag ccagcatggt ccgtttgata ttccctgtca
     5701 taaaagcgtt attataggca atacccgata cttctccaaa agcagttgtc accagtccca
     5761 tacagaaggc caagggcggc actagataga tattatccac agtttgcggc acaaaagcaa
     5821 taatgattga taagattgcc aagggaatca aggacagaat aggttttttc acaattctca
     5881 atttttcctt ataaatcgtt aataaaaaga ctcccatcat aaacgctagc aaggtgagaa
     5941 ccttgtccct aacatccgaa acattatttt taattaattc tactgaaaga aagacaacat
     6001 ttccagtttg tccagctaca agggtattcc cgcgaacaat aaaagtgtaa gcatccacat
     6061 atccagcaca aaacgtcaaa aaaagtgcta accttttaga ctgacgtgat atttttctta
     6121 taggtaataa cctcatttta cctcccattg tattttctct tagaaatatt gtaccatttt
     6181 ctttctaaaa aatcgtaggc taccatttag attttactat tagcataaaa ataataatag
     6241 acaactattt atccaaaaat agatagatgt aacatgtttg caaacaaagc atacgaacct
     6301 ttagtaaaat catttccatg aaactagaat agagccctct tagcaaaaat cattatttta
     6361 atttatttct aatcactcct tgacataaat aactctcacc aataaaagac tatgtcttaa
     6421 aaaaatggta taataaaatc aatacttggg cttgatggct atgctactaa taacaattag
     6481 gagagaaaat caggcacttg ttaacaacaa ggattatccc cttgagatga aaggaacttt
     6541 agaaatctta tgatgaacat gcaaaacatg atgcgtcaag cacaaaaact tcaaaaacaa
     6601 atggaacaaa gccaagctga acttgctgct atgcaatttg ttggcaaatc tgctcaagat
     6661 cttgtccaag cgaccttaac tggcgataag aaagttgtca gcattgattt caatccagct
     6721 gtcgttgacc cagaggacct tgagactctt tctgatatga ccgttcaagc catcaactct
     6781 gctcttgaac aaatcgatga aactaccaag aaaaaactgg gtgctttcgc tgggaaatta
     6841 cctttctaaa aacaaggagc tagaacaatg cttgtcgata acaaaggcta agaaaggtgc
     6901 aaaaatgact ctataatatt tgtagtgggt aaatccccta tggatattat ggagcctatt
     6961 tttgtgta
//
LOCUS       DAVKWZ010000079         6740 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000079 DAVKWZ010000000
VERSION     DAVKWZ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6740
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.079"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(91..354)
                     /locus_tag="VW854_001909"
                     /pseudo
     CDS             complement(91..354)
                     /locus_tag="VW854_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            548..2314
                     /gene="patB"
                     /locus_tag="VW854_001910"
     CDS             548..2314
                     /gene="patB"
                     /locus_tag="VW854_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEV0263093.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSAGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            2453..3142
                     /locus_tag="VW854_001911"
     CDS             2453..3142
                     /locus_tag="VW854_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV0263094.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            3146..3325
                     /locus_tag="VW854_001912"
     CDS             3146..3325
                     /locus_tag="VW854_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263095.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            3312..4661
                     /locus_tag="VW854_001913"
     CDS             3312..4661
                     /locus_tag="VW854_001913"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEV0263096.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            4785..6245
                     /locus_tag="VW854_001914"
     CDS             4785..6245
                     /locus_tag="VW854_001914"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEV0263097.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWIIQKKDGYPTYNFAVAIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDKFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            6259..6693
                     /locus_tag="VW854_001915"
     CDS             6259..6693
                     /locus_tag="VW854_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV0263098.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAEDGQIFTGF
                     CMEGTCGVFHLCAERAALFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNER"
BASE COUNT         2108 a         1303 c         1448 g         1881 t
ORIGIN      
        1 gtaatgtttt ccttattcta acttagtctt aagataggcc ttaaaagcag gtgaaaagcg
       61 agcgcatgct ttggcagctt gtatgagtgc ctaccgcaga tgaggggaac cccgtttgat
      121 cattcttcca gctaaatcaa tctgacctga ctgataaata gaagaatcca gtccagcgaa
      181 agcttgtaat tgagcaggat tatcaaaggc atgaatattt tgaatctcgg ctaaaatgac
      241 cgcccctaaa cgattcccaa tcccagtaac cgtcgtgatg accgaattta actcagccat
      301 caagtcattg acacattttt ccgccttgtc aatgagcctc ttgtaatgtt tgatgttttc
      361 attacacgag ataaaacgtc tatgcgttat caaactcatt accaattaaa acaaatgtgg
      421 ttagatcctt tcggaaattg tcaagcgatt ggaggaaatg aactaatcca cagcggctta
      481 ttccaagtat accacttggg ctttggcagt agctaactgc gctaaatata atataaggag
      541 gagtaaaatg aagacagttc aatttttttg gcattatttt aaggtctaca agttctcatt
      601 tgtagttgtc atcctgatga ttgttctggc gacttttgcc caagccctct ttccagtctt
      661 ttctggacaa gcggtgacgc agctagccaa tttagttcaa gcttatcaaa atggcaatcc
      721 agaacttgta tggcaaagcc tatcaggaat catggtcaat cttggcctgc tggttttggt
      781 tctatttatc tctagtgtaa tatacatgtg tctcatgacg cgcgtgattg cagaatcgac
      841 caacgagatg cgcaaaggtc tctttggtaa gcttgctcag ttgacggttt ctttctttga
      901 ccgtcgacaa gatggcgata tcctgtctca ttttaccagt gatttggata atatcctcca
      961 agcctttaac gaaagcttga ttcaggtcat gagcaatatt gttttataca ttggtctgat
     1021 tcttgtcatg ttttcgagaa atgtgacgct ggctctcatc accattgcca gcaccccatt
     1081 ggctttcctt atgctgattt tcatcgtgaa aatggcacgt aaatacacca acctccagca
     1141 gaaagaggta gggaagctca acgcctatat ggatgagagc atctcaggcc aaaaagccgt
     1201 gattgtgcaa ggaattcaag aggatatgat ggcaggattt cttgaacaaa atgagcgcgt
     1261 gcgcaaggca acctttaaag gaagaatgtt ctcaggaatt cttttccctg tcatgaatgg
     1321 gatgagcctg attaatacag ccatcgtcat ctttgctggt tcggctgtac ttttgaatga
     1381 taagtctatt gaaacaagta cagccctagg tttgattgtt atgtttgcac aattttcaca
     1441 gcagtactac cagcctatta tccaagttgc agcgagttgg ggaagccttc agttggcctt
     1501 tactggagct gaacgaattc aggaaatgtt tgatgcagag gaggaaatcc gacctgaaaa
     1561 ggctccaacc ttcactaagt tgcaagaaag tgttgaaatc agtcatatcg atttttcata
     1621 cttgcctgat aaacctattt tgaaagatgt cagcatttct gcccctaaag gccagatgac
     1681 agcagttgtt gggccgacag gttcaggaaa aacgactatt atgaacctca tcaatcgctt
     1741 ttatgatgtt gatgctggtg gtatttattt tgatggtaaa gacattcgtg gctatgactt
     1801 agatagtctt agaagcaagg tgggaattgt attgcaagat tcggtcttgt ttagcggaac
     1861 gattcgagac aatatccgat ttggtgtgcc agatgctagt caggaaatgg ttgaggtagc
     1921 agcaaaagca acccacattc acgactatat cgaaagtttg cctgataagt acgatactct
     1981 tattgatgat gaccagagca tcttttcagc agggcagaag caattgattt caatcgctcg
     2041 aaccctgatg acagatccag aagttctcat tctcgatgaa gcaacttcaa acgtagatac
     2101 ggtgacagaa agcaagattc aacatgccat ggaggtggtt gtagcaggta gaactagttt
     2161 cgtcattgcc caccgcttga aaaccattct caatgcagat cagattattg tccttaaaga
     2221 tggagaagtc attgaacgtg gtaaccacca tgaacttttg aagctaggtg gcttttattc
     2281 agaactctat cacaatcaat ttgttttcga ataagaaaga agttgtccta tgtgggcagc
     2341 tttttcttgt ccataaaaaa tgtttatcac agccttaaaa aaaacatatt agacgaaagt
     2401 cattttgagt gatatgatag gactatcgtt agcattcgaa aggagaggca tcatggctag
     2461 aacggttgta ggagttgctg caaatctatg tcccgtagac gcagaaggca aaatcattca
     2521 ttcatctgta tcttgtagat tcgcagagat cattcgtcaa gtcggtggtc tccctttagt
     2581 cattcctgtt ggtgatgagt cagttgtacg tgattatgtg gaaatgattg acaaactcat
     2641 tttgacagga ggccaaaatg ttcatcctca gttttatgga gagaaaaaga ccgtcgagag
     2701 cgatgattac aatctggtcc gtgacgaatt tgaattggca ctcttgaagg aagcgcttcg
     2761 tcagaataaa ccaattatgg caatctgtcg cggtgtccaa cttgtcaatg ttgcctttgg
     2821 tggaaccctc aatcaagaaa tcgaaggtca ctggcaagga ctacctttcg ggacatctca
     2881 ctctattgag acagtagaag gaagcgtggt ggctaagcta tttgggaaag aaagtcaggt
     2941 caactcagtc catcgtcaaa gcattaaaga tttggcacct aatttccgtg taactgctat
     3001 tgattcaaga gaccaaacta ttgaagcgat tgagtctatc gacgagcacc gcattatcgg
     3061 tttacagtgg catccagagt ttctggttaa tgaagaagat ggcaatttag aattatttga
     3121 gtatttattg aatgaactgt aacgattgga acatctggtc gttctttctt ttattttttc
     3181 aaaattttta gaatgtggca ttttacgcaa acgtttgaat tctgataaaa attggagaaa
     3241 ttcgacaaaa aaacttgaaa aaaacgaagg taagcgttat gatagaaaag aagaaatatt
     3301 ggaggaagaa catgtcacat attaaatttg attattcaaa agttttagac aaatttgttg
     3361 caccacatga agtggaatac atgcaatcac aagtaacagc agcagatgaa ttgatccgta
     3421 aaggaactgg tgctggtagc gacttcttgg gatggttgga ccttcctgaa aaatacgacc
     3481 gcgaagaatt tgaccgcatc ttgaaagctg ctgagcaaat caagtcagac agcgatgttt
     3541 tggttgtgat cggtattggt ggatcttacc ttggtgcaaa agcagcaatc gacttcttga
     3601 atcaccattt tgctaacttg caaacaaaag aagaacgcaa agctccacaa atcctttacg
     3661 caggaaactc aatctcatct acttaccttg ctgacttggt agagtatgtt gcagacaaag
     3721 acttctcagt aaacgtaatt tctaaatcag gtacaacaac tgaaccagcg attgctttcc
     3781 gtgtctttaa agaactcttg gttaagaaat acggtcaaga agaagctaac aaacgtatct
     3841 atgcaacaac tgaccgccaa aagggtgctg ttaaggttga agcagacgct aacggttggg
     3901 gaacatttgt tgttccagat gatatcggtg gacgcttctc agtattgaca gccgttggtt
     3961 tgcttccaat cgcagcatca ggagctgaca tcaaagctct tatggaaggt gcgaatgcag
     4021 ctcgcaaaga ctacacttca gacaaaatct ctgaaaacga agcttaccaa tacgcagctg
     4081 ttcgtaacat cctttatcgt aaaggctatg caactgagat cttggtaaac tatgagccat
     4141 cacttcaata cttctcagaa tggtggaaac aattggctgg tgaatcagaa ggaaaagacc
     4201 aaaaaggtat ctacccaact tcagccaact tctcaactga cttgcactca cttggtcaat
     4261 ttatccaaga aggaactcgt atcatgtttg aaacagttgt ccgtgttgac aaacctcgta
     4321 aaaacgtgct tattcctact ttggaagaag accttgacgg acttggttac cttcaaggaa
     4381 aagacgttga ctttgtaaac aaaaaagcaa ctgacggtgt tcttcttgcc cacacagatg
     4441 gtgatgtacc aaacatgtat gtgactcttc cagagcaaga cgctttcact cttggttaca
     4501 ctatctactt cttcgaattg gcaattgccc tttcaggtta cttgaatgct atcaacccat
     4561 ttgaccaacc aggtgttgaa gcttataaac gtaacatgtt tgcccttctt ggaaaaccag
     4621 gatttgaaga attgagcaaa gaacttaacg cacgtctata atagaagaaa agagtggttt
     4681 gcccactctt tttactctct ttatccatag aaattggact cagccaagac ttgtgatata
     4741 atatagaaag caaaaaggca gacgcctaga taataggaga aactatgtca aaagatatcc
     4801 gcgtacgtta cgcaccaagt ccaacaggac tactacacat cggaaatgct cgtacagcat
     4861 tgtttaatta cttgtatgcg cgccatcatg gtggaacatt tctcatccgt atcgaagata
     4921 ctgaccgtaa acgccatgtc gaggatggtg aacgttcaca acttgaaaac cttcgctggt
     4981 taggcatgga ttgggatgaa agtccagaat cacatgagaa ttatcgccag tctgagcgtt
     5041 tggacttgta tcaaaaatac attgaccaac tattagctga aggaaaagcc tataaatctt
     5101 acgttacaga agaagagttg gcagctgaac gcgaacgcca agaagtagct ggcgaaacac
     5161 cacgctacat caatgaatac cttggtatga gtgaagaaga aaaagcagct tacatcgcag
     5221 aacgtgaagc agcagggatc atcccaactg ttcgtttggc tgtcaatgag tcaggtatct
     5281 acaagtggca tgatatggtc aaaggcgata tcgaatttga aggtggcaat atcggtggtg
     5341 actggattat ccaaaagaaa gacggttacc caacttacaa ctttgccgtt gccatcgatg
     5401 accatgatat gcaaatctct catgttatcc gtggagacga ccatattgct aacacaccaa
     5461 aacagctcat ggtttatgaa gcacttggtt gggaagctcc agagtttggt cacatgacct
     5521 tgattatcaa ctctgagact ggtaagaagt tatctaaacg tgatactaat acacttcaat
     5581 ttatcgaaga ttaccgaaag aaaggttacc ttccagaagc agtctttaac tttattgctc
     5641 ttcttggttg gaacccaggt ggtgaagatg agattttctc tcgtgaagaa ctcattaaac
     5701 tttttgatga aaaccgcctc agcaagtcac cagcagcctt tgatcagaaa aaactagact
     5761 ggatgagcaa tgattatatc aagaatgcag accttgaaac catctttgaa atggcaaaac
     5821 cattcttaga ggaagcaggc cgtttgactg acaaggctga aaaattagtt gagctctata
     5881 aaccacaaat gaaatcagta gatgagatta tcccattgac agatcttttc ttctcagatt
     5941 tcccagaatt gacagaagca gagcgcgaag tcatgacggg tgaaacagtt ccaacagttc
     6001 ttgaagcatt caaagcaaaa cttgaagcga tgacagatga taaatttgtg acagaaaata
     6061 tcttcccaca aattaaagca gttcaaaaag aaacaggtat taaagggaaa aatcttttca
     6121 tgcctattcg tatcgcagtt tcaggcgaaa tgcatgggcc agaattacca gatacaattt
     6181 tcttgcttgg acgtgaaaaa tcaattcagc atatcgaaaa catgctaaaa gaaatctcta
     6241 aataaagagg atacaataat ggacatctgg gaaaagatgt acgaagaagc acagaaacta
     6301 tacaatccac atgaagtatc agactttgtt tatgccaatc atgttgtagc tgcagtagaa
     6361 gcagaagatg gacaaatatt tacaggattc tgtatggagg gaacctgtgg tgttttccat
     6421 ctctgcgcag aacgggcggc cctcttcaat atgtaccaat tttcaggaca aactaaggtt
     6481 aagaaagtat tagcctttcg agacaaacca ccttatggtg gaagttcagc catgccttgc
     6541 ggtgcttgta gagaattcct tttagagctt aatgctgaaa ataaagatgc agaattcatg
     6601 atggactata atataagaaa aacagttaaa gtcgcagaac taatccctta ttggtgggga
     6661 gaagaacgtg cttctaagtt taatgagcga tagaagagtc agtataattc tggctttttt
     6721 tactataagt tgaaaaaata
//
LOCUS       DAVKWZ010000080         6658 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000080 DAVKWZ010000000
VERSION     DAVKWZ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6658
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.080"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     regulatory      complement(85..173)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(382..1011)
                     /locus_tag="VW854_001916"
     CDS             complement(382..1011)
                     /locus_tag="VW854_001916"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV0263099.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKD
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ"
     gene            complement(1013..1795)
                     /gene="thiM"
                     /locus_tag="VW854_001917"
     CDS             complement(1013..1795)
                     /gene="thiM"
                     /locus_tag="VW854_001917"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV0263100.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            complement(2032..2700)
                     /locus_tag="VW854_001918"
     CDS             complement(2032..2700)
                     /locus_tag="VW854_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEV0263101.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     regulatory      complement(2915..3003)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3255..4391)
                     /gene="lctO"
                     /locus_tag="VW854_001919"
     CDS             complement(3255..4391)
                     /gene="lctO"
                     /locus_tag="VW854_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEV0263102.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     gene            complement(5016..6506)
                     /gene="lysS"
                     /locus_tag="VW854_001920"
     CDS             complement(5016..6506)
                     /gene="lysS"
                     /locus_tag="VW854_001920"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEV0263103.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLITKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
BASE COUNT         1986 a         1420 c         1141 g         2111 t
ORIGIN      
        1 cagtttcgta aaagaaaaaa ctctaattac agatacgaat tagagttcag cttacaagat
       61 tagacagttc ttttcgacat acgaaaaaaa catttcacat ttcccttcgc cagtcttaac
      121 tgtatcaggt tcaatgggta tcatctcagc ctaaagcacc ccaaatgcct ttattattta
      181 attatgtgat tattataaca cacattttat actagttcaa gaaattgaac tggaaataca
      241 gccttgcact cacaaagaca gcagatcttt cttttgcaaa aaacaaatga cctgtttgat
      301 gaattaacca ttcaagctga atctagacat agctttttaa aaaaggaaaa tcctacttac
      361 ttagaatcca aggatagata tctattgctc actcatttcc cgaacagttt tttctatatt
      421 ttttgcatac gatattgccg aaatgattga aacgccatcc atattggtct ttataatgtc
      481 tttaatatgt ttcgtctgta tcccaccaat tgcaactaaa ggcatttgtg gcaatagttt
      541 tctcatcaat tcaagacctt cataacctat agcaccacca gcatcatctt ttgactgggt
      601 atcaaataca ggcccaacac ctacataatc tacatattca acttttgatt gttgaaattc
      661 ttcttcgttt cttatagaaa gaccaattat tttatctggc atcaattttc taatttcatc
      721 aacaccaata tcatcttgac ctacatgtac gccatcggcg tcaatttcca ttgctaaatc
      781 tatatcgtca ttaacgataa atggaacatt gtatttttta caaagttctt taatttggat
      841 agctagctca agtttttcta agccttctaa agcaccctca tctttttctc gaaattgaaa
      901 taaggttata ccacctttta aggcttcctc aacgactgta tatagatttt ttccttggca
      961 agtagtcgtt ccacaaataa aatatagttt tagtaattct ttatgaaaca tcttacttca
     1021 ctcttttgaa ttcctttaca tcttcatctg taatctcgta taaggcattt ataaattcaa
     1081 ctttaaatgt cccaggaaga tgtccatttg gacgtttttc tgccatttct ccagcgatat
     1141 tgtaaactaa cattgctgtt tctaatgatt tcaattcttg acctttttct agtccgataa
     1201 agcttgctac tacagctcct aataagcatc ctgtcccaat gactttcggc atcatagcac
     1261 taccattatg aatcgttacc acttctccat taacagcaat ggcatccact tcacctgtta
     1321 ctactattgg aatattgaac ttctcatttg ctgctagagc aatctcgtca atattatcta
     1381 ctcccgcact atctactcct ttagatgcca catctattcc tactaaagag gcaatctctc
     1441 cagcattccc cctaatcgca gctagtttgt aattgttgat tagatcatct gctacttttt
     1501 ttctatattc tcctgctcca caggctacag gatctaaaac tgctgggaca ttatatttct
     1561 ctgcaatttt cagagcagct tggtataatt tccaattttc atctgtcaat gttcctatgt
     1621 ttattaataa accaccagca tactttaata agtcctctaa atctgctgga aactcactca
     1681 tggctggtga ggcgcccagt gctactaatc catttgctgt gaaatttttt actacatcat
     1741 tggtgatgca aatgaccaaa ggtgcttttt cttttaataa ttttaaactt gtcatattga
     1801 aatccttcct tttcacttta tacgatctac taatttcgat ttatctttag ttgagaattt
     1861 ttttcattta cattgaatga tttatactca atgaaaatca aagagcaaac taggaggcta
     1921 atcgcaggtt gctcaaaaca ctgttttgag gttgtggata gaactgacgt ggtttgaaga
     1981 gattttcgaa gagtcttacc tcatcaaatt tgtaaatatc atgagccttc tctagacatc
     2041 gtaaccaata tcaaaaaaag ctaattctaa agcgactgct tgattccagc gttgctgaag
     2101 ttctgtcaaa tcttctcgat ttttaccgac acgattgagt tcgtcaacca gaaattgaac
     2161 ccactctgca aagaaaggac ctctgtggag attgatccat tccgaatgaa tatagacttc
     2221 aggtaaagcc aaatctttag aaccccagtc taaatagaga ccttctgcaa tgaccagcat
     2281 gaccaaaaga tgggcatagt ctgatgaagc caccgccgaa tacattagat cctgaaaggc
     2341 ttttgttaca gggtgcaaag tcacttctag atagtcattc tctgctactt ttaactcttt
     2401 aaaagccttt tggaaataac catcttcatc tgcttcaaga aagcctagtt gcttggcaaa
     2461 acgaagcttg gattcaagtt tatctgcgtg agctacgcag gcacccagca tggataagaa
     2521 ggcatcaaag aagtgataat cttgaatcag atagtccttt aagaccttat tctcaattgt
     2581 ccccgcaaaa agttccttaa caaaacgatg attgattgca gcctgccaat ccttctgact
     2641 gctttttaat aattctccaa cagtcaaacc tggctgaaat gcatagtctt gtgtttccat
     2701 atttacttct cctctcttta cttgttagta attaataaaa cagcaagaaa tatcaagcaa
     2761 aatcgtaatt ccacttgatc cttttaaagc acatcgagag catttacaga gagctaacta
     2821 aacaagccta tccagtttat ataaacaaaa aactccaatt acaatcaaga attagagttg
     2881 acttacaaga ttagatcgtt catttcgcca tacgaaaaaa cgtttcacat ttcccttcgc
     2941 cagtcttaac tgtatcaggt tcaatgggta ttatctcagc ctaaagcacc ccaaatgtct
     3001 ctattattta attactgaac cagtatagca aaaaatgaaa gccctagcaa gatatttgac
     3061 cgaaaaatat ctttatatag tagattgaaa ctagaatagt acacctctac ttctaaaaca
     3121 ttgttagaaa tcgatttgac tgtcctgatt gatttgtcct attcttattt cattttacta
     3181 tagttttcga tagcaattta ttcttccaat acacgaagaa aaacctccac attcagtgga
     3241 ggcaatctgt tttatcaata caattttaag tcacgagggt caactgggaa ggttgggttg
     3301 tatggattgt gacggagctt aaagtgtttg acatcttcaa tggtctgagc tccagacaat
     3361 tgcataactg tcttcaattc cgcattcaag tgttcaaaga cttgacgcac accgacacta
     3421 ccaccgagag ccaagccata aataacaggg cgaccaatag ctaccaagtc tgctcctgaa
     3481 gccaaggctt taaagacgtg ttgaccacga cgaacaccag agtcaaagac aatcggcaca
     3541 cgtctatcaa ctgcttctgc cacttcttga agcgagtcaa aggcagctgg tccaccgtcg
     3601 atttgacgac caccgtggtt ggttacccag ataccagaag ctcctgcagc aagcgaacgt
     3661 tcaacgtcct cacggcattg tggtcccttg acatacacag gaagaccaga gtattcagcg
     3721 ataaattcta catcgcgtgg agacaagcgt tgtttagctg atttgtaaac aaagtccatt
     3781 gatttaccag caccttctgg caggtattct tcaacaatcg gcatgccaac tgggaagaca
     3841 aaaccattac gcttatccac ttcacgattc ccccctacag tagcatctgc cgtcaagaca
     3901 atcgctttat aaccttcagc cttcacacgg tccatgatgt ggcggttgat accgtcatcc
     3961 ttactaaagt aaaattggaa ccaatggggg gtcccttgaa gggcttcaga aatctctgga
     4021 aggtcaacag tagagtaaga gctggttgta taaagagaac caaactcatg cacaccacgc
     4081 gcagtcgcca cttccccctg ttcatttgcc aatttatgag ccgcaacagg cgccataatg
     4141 attggtgaag atagtttttc acctgcaaat tcaatctctg tacttggatt ttcaacatcg
     4201 caaagtgtat gaggaacgat gagtttgtgg ttaaaggcgc ggatattctc tctcaaagtg
     4261 aaagtatctt ccgccccact agcgatatag ccaaatgctg ctttaggaat aacttgttgc
     4321 gccattggct ccaaatcata ggtattgatg aaatctacat gaccttctgc attgcttgtt
     4381 ttgtatgaca taaactgtcc cccttaatta agtaagcgtt tgctttttac tatacaacta
     4441 taataactct tttttcaatc attttcaaga gttttgctta aaaaccatct tattttttca
     4501 tacaatatac tagccctact ttccttaaac tctaaacttt agtctatatt aagtgaatcg
     4561 ttgagatttt ttgctgccag gcatttaaac tactttttta tttgctgatt ctatctacta
     4621 atactataaa aaaacaagtg aataagacac cagaacatcc tctaatagga cctatggact
     4681 tatatcagtc ccatatatac tgtttaagca agatactatc ctagttgacg ctaacttcct
     4741 atcccactta ttaatttttt atttatagtg aatctgaaca aatcaattgg ggaatccaag
     4801 tcaatttcta gtactttttg aacagtttca gcgtactatc gtaaaatgaa ataaaacatg
     4861 cacaaattgc ttagggaatt taaaccaatt tctaagaatg tcttagaaat caaagtgtac
     4921 tatttcaact tcaatatact acacttcatt ttctccttaa attcagtctc tttttgaatc
     4981 aaaaaatccc ctgataagag ccagaggata agaatttatt tcattgttgg gaagagcaat
     5041 acatcacgga tagttgttgt atcagtgagg agcatgcaga gacggtcgat accgattccc
     5101 aaaccacctg ttggtggcat accgtattca agggcttcaa tgtagtcata gtcgattcct
     5161 gtcgcttcat catcaccaag ttctttggct ttagcttggg cttcaaaacg gctaagttgg
     5221 tcgattgggt cgttgagctc agtaaaggca ttaccgtact ccttagtcat gataaagagc
     5281 tcgaaacggt cagtaaagcg ttggtcttca ggatttttct tagcgagtgg agatacagct
     5341 actggatgtc catagacaaa ggttggttgg attaaagttt cttcaacaaa ctcttcaaag
     5401 aaggcattga tgatgtgacc aacctcagtg tagtgtttct caactggaac tttcttctca
     5461 gcagcgatag ctttagcttc ttccaaagtc atgtcttgcc agaaatcgac accagtaatt
     5521 tctctgatag catccaccat atgaacacgc ttaaatggtt cgttaatctt gatttcagta
     5581 ccttggtagt taactgggcc atcgccctta actgatttag cagcgtgttg gataatgcct
     5641 tccgtcaagt ccatgatgtc ttggaagtct gcataagctt ggtaaacttc gatagaagta
     5701 aactcagggt tatgagtagc gtccattcct tcgttacgga agatacggcc aatttcatag
     5761 acacgttcca taccacccac gataaggcgt tttaagtgaa gttcagtcgc gatacgaagc
     5821 accatgtcaa tgttttgggc attgtggtgg gtgataaatg gacgggcagc agcaccacca
     5881 gcttcgttgt gaagaacagg tgtttccact tcaaggaatc ctttttggtc aaggtaacga
     5941 cggatttcag agatgatttt tgaacgagtg acaaaacgtt caaagctttc acgattagaa
     6001 atcaagtcaa ggtaacgttt acggtaaatt gtttcaacgt ctgtcaaacc atggaatttc
     6061 tcaggaagag gacgaagagc cttagacaag tgtgtgatgt gggttgcctt gatagagagt
     6121 tctcccatat ccgtacgcat cacttcacct tcgacaccaa ggaagtcacc aagatctgct
     6181 tttttgaaga tttcgtagtt ttcttcaccg acagcatcct tacgaacgta gatctgaatc
     6241 tggccttcgc ggtcttgaag gtgggcaaaa ccaacttttc ctttaccacg tttggttatc
     6301 aagcgtcctg cgatagtagc tgtttcgttt ttatcgtgta attgttcttt atcgaggttg
     6361 gcatatttat cttttaattc ttgtgaattt gcagtacgtt caaaacgttt tccgaaagga
     6421 tcgattcctt gttcgcggag cgcagccatt ttttcacggc gaacgatctg ctggtcattt
     6481 agttcttcca tatgttctgt agacatggga tcctcctagt tttactcaaa attgaatacg
     6541 atgagtcatt ttttgcgata ctcccatttt atcataaata agcaagaaat tcacggataa
     6601 tctttaaaaa ctaaaaagaa cctgacgcta aaatcactgg aaagaagtga aagtatct
//
LOCUS       DAVKWZ010000081         6307 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000081 DAVKWZ010000000
VERSION     DAVKWZ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.081"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            1..500
                     /locus_tag="VW854_001921"
                     /pseudo
     CDS             1..500
                     /locus_tag="VW854_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            667..867
                     /locus_tag="VW854_001922"
     CDS             667..867
                     /locus_tag="VW854_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263104.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            909..1313
                     /locus_tag="VW854_001923"
     CDS             909..1313
                     /locus_tag="VW854_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263105.1"
                     /translation="MDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETNSVPA
                     GVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKAQNLL
                     QETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            1544..2251
                     /gene="ccdA2"
                     /locus_tag="VW854_001924"
     CDS             1544..2251
                     /gene="ccdA2"
                     /locus_tag="VW854_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEV0263106.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYVMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            2262..2819
                     /gene="sdbB"
                     /locus_tag="VW854_001925"
     CDS             2262..2819
                     /gene="sdbB"
                     /locus_tag="VW854_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEV0263107.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGQEETKKTQAAQQPKQQTTVQQIS
                     VGKDVPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            3193..4584
                     /locus_tag="VW854_001926"
     CDS             3193..4584
                     /locus_tag="VW854_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV0263108.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKVPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            4727..5644
                     /gene="adcAII"
                     /locus_tag="VW854_001927"
     CDS             4727..5644
                     /gene="adcAII"
                     /locus_tag="VW854_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEV0263109.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLAEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            5652..>6307
                     /locus_tag="VW854_001928"
     CDS             5652..>6307
                     /locus_tag="VW854_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV0263110.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRYQAGQDKKESNRVAYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKDAAHADNIRTKEEIKRQKQEH
                     SHNHGGGSNDQAVVAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGNHFHYIPKSD
                     "
BASE COUNT         1948 a         1126 c         1331 g         1902 t
ORIGIN      
        1 taaaccacca agtaaaagga acaaaaccaa gaaaagttga tagagataga cttaaaaact
       61 atcttactga caatccagac gcttatttga ctgaaatagc ttctgaattt ggctgtcatc
      121 caactaccat ccactatgcg ttcaaagcta tgggctacac tcgaaaaaaa gaaccacacc
      181 tactatgaac aagacccaga aaagtagcct tatttcttaa gaattttaat agtttaaagc
      241 acctagcacc tgtttagatt gatgaaacag gattcgatac ttatttttat cgagaatatg
      301 gtcgctcatt aaaaggtcag ttaagaagag gcaaagtatc tggaagaaga tatcagagga
      361 tttctttggt tgcaggtcta acaaatggtg aattaatcgc tccaatgact tacgaagaga
      421 tgatgacgag cgactttttt gaagtatggt ttcagaagtt tttcttacca acattaacca
      481 caccatcggt tattatagta aaatgaaata agaataggac aaatcgatca ggacagtaaa
      541 atcgaattct aacaatgttt tagaagtcca tatgtactat tctagtttca atataccata
      601 actgttccta gtcaacctgc aagccctgtc ccagctccaa gctcagctcc gactcaagat
      661 cctagagtgg tagcaagcgc ttcggctagc tcaacgagca cacaagctca agagcaagtt
      721 gacaagtctg aacttcgtgc cttgagtcaa gagttagacc aacgcttgaa agctttggca
      781 acagtatctg atccgaaaat cgatgcaacc aaggctgtcc tcctagatgc tcaaaaagct
      841 ccggaagata gtgccttgac agagtaaggt ctgcatactg cagtagagtc tgtgaaggct
      901 gggctgcctt ggactctctc aaagatgtga aagcaaatgc tagcgacagc aagcctgcac
      961 aggacaagaa ggatgcaaaa caaggaacgg aagatagtaa ggattcagat aagatgactg
     1021 aaacaaactc agttccggca ggagtgattg tggtcagtct acttgccctc ctaggcgtga
     1081 ttgccttctg gctgattcgc cgtaagaaag agtcagaaat ccagcaatta agcacggaat
     1141 tgatcaaggt tctaggacag ctagatgcag aaaaagcgga taaaaaagtc cttgccaaag
     1201 cccaaaacct tctccaagaa acccttgatt tcgtgaaaga agaaaatggc tcagcagaga
     1261 cagaaactaa actagtagag gagcttaaag caatccttga caaactcaag taaaacatga
     1321 tttatgaaat gtcagaccta aaagacattt tcgttcacaa aagcagcgat gagtaatcct
     1381 tgtcgttgct ttttggctat tttgggggat aagatccgcc ttgactagca aaagaaaaga
     1441 ttggtccaat agtgcccgat acgattttgc gtagcagaaa gcttacaaat aaaattgaat
     1501 atagtaaaat aggattagaa ttattaagaa agttggttct ttattggaaa cgatagtatt
     1561 tttaatctct gtttttctag caggtgtttt atcctttttt tctccttgta tttttcctct
     1621 tctgccagtc tatgctggga ttttattgga tgatcaagaa agtgcaaaaa gcttttcttt
     1681 gtttgggaga aaggttctct ggtcaggctt gattcgaaca ctttgcttta tcgctggtat
     1741 ctctctcatt ttctttattc taggctttgg tgctggttac tttggtcata ttctctatgc
     1801 aaattggttt cgatatgtca tgggagctat tattatcatt ttgggtcttc accagatgga
     1861 aatttttcat ttgaagaaat tagaagttca aaaaagtttt acctttaaaa aatcagattc
     1921 taatcgttat tggtcagctt ttttacttgg tattaccttt agctttggtt ggacaccttg
     1981 tattggtcca gttttaagtt ctgttttagc acttgcggct tctggaggca atggtgcttg
     2041 gcaaggagcg atttatactc tcatttacac tctgggcatg gcccttcctt tcttggtctt
     2101 ggcactagct tcaggtctag tcatgccata ttttagtaaa atcaagcgtc atatgatgct
     2161 actaaagaaa attggtggtt tcctcattgt tttaatggga attttgttac tattaggaca
     2221 agtaaatgtt ctagctggaa tttttgaata aaggaggaca aatgaaaaaa gtaatgtttg
     2281 ctggcttaag tctcttgtca ttagttgtat tgatggcctg tggtcaggaa gaaactaaaa
     2341 agactcaagc agcacaacag ccaaaacaac aaacgactgt acaacaaatt tctgttggaa
     2401 aagatgttcc agactttaca ttgcaatcca tggatggcaa agaagttaag ttatctgatt
     2461 ttaagggtaa aaaggtttac ttgaagtttt gggcttcatg gtgtggtcca tgcaagaaaa
     2521 gtatgccaga gttgatggaa ctagcggcga aaccagatcg tgatttcgaa attcttactg
     2581 tcattgcacc aggaattcaa ggtgaaaaaa ctgttgagca attcccacaa tggttccaag
     2641 aacaaggata taaggatatc ccagttcttt atgataccaa agcaaccacc ttccaagctt
     2701 atcaaattcg aagcattcct acagaatatt taattgatag ccaaggaaag attggaaaga
     2761 ttcaatttgg tgctatcagt aatgcggatg cagaagcagc atttaaagaa atgaactaga
     2821 atcgataaaa tctgattaca agatgtagtc agattttggt aacttctaaa actaacttcc
     2881 agtttcccac aacctagctt ttagtatgag aaaaacgcgt gacatcagtg gaaatccgtc
     2941 ttagactaac ttccactggt tttctttttc ttgatatata agggttcaaa agcgaaaaga
     3001 ctcagaaaaa gtgcacgaca aatagcccta aaacagagtc gtcttagagt aacttccagt
     3061 tttgggagag agatggaagt tactttgaga agttacggta gtcagatttt tttagaaaaa
     3121 cattttataa atattcacat atctcctata tttatggtaa aatagaatta tcagtttatt
     3181 ttggagtcaa agatgaatat atttagaaca aagaatgtta gtttagataa aacagagatg
     3241 cataggcatt tgaagttatg ggatttgatt ttgctgggta tcggagccat ggtagggaca
     3301 ggcgtcttta caatcacagg tactgcagct gcaacacttg ctggcccagc cctagtgatt
     3361 tcaatcgtta tttctgcctt gtgtgtggga ttatcagccc tcttttttgc agaatttgcc
     3421 tcgcgagtac ccgctacagg aggtgcctat agttacctct atgctatctt aggagaattc
     3481 cctgcctggt tggctggttg gttaaccatg atggagttca tgacagccat atcaggcgta
     3541 gcttcgggtt gggcagctta ttttaaaggc ttgctctctc aatacgggat agcctttcct
     3601 caggctttaa atggtacctt taatcctcaa gcagggacat ttgttgattt attgcctatt
     3661 cttgtcttgg tcttggtgac ttcgcttgtt ttactaaatg ccaaggcagc cttacgcttt
     3721 aattcgattc tagttatttt gaaattttcc gctttagctc tctttgtttt agtaggaatt
     3781 tggaatatta agtttgataa ttggagcaat tttgctcctt acggttttgg acaaatctat
     3841 ggagctagta ctggtattat ggctggtgca tccttgatgt tcttcggttt tttgggattt
     3901 gaatccatct ctatggctgt agatgaagtc aagactcctc aaaaaaatat tcctagaggg
     3961 attgtcttat cgctttctat cgtaaccatt ctctatgcct tggtgaccct tgttttgact
     4021 ggtgtggttc actatagtca tttaaatgtc gatgatgccg ttgcctttgc ccttcgtagt
     4081 gttgggatta gttgggcagc caactatgtg tcattagtgg ctatcttgac cttgattaca
     4141 gtttgtattt cgatgaccta tgccctatcg cgtatgattt acagtttagc acgtgacggc
     4201 ttagtgcctg ctgcctttaa ggaactgacg aagactagca aggtaccaaa gaatgctact
     4261 atcttgacag gtctagcttc agcagtagca gcaggaatgt tcccactagc cagtattgca
     4321 gcctttttaa atatttgtac cttagcctac ttgatcatgt tggcttatgg tctgattcgc
     4381 ttacggaaag aaaaaggaat gcccaaagct ggggaattta aaacaccatt ggtacctctg
     4441 ttaccaattt tatcgatcat catttgttta tccttcatgt tacagtataa tatgaatacc
     4501 tggattgctt tcctagttgc gttgttagta ggaagtatta tttacttcac ttatggctat
     4561 aagcattcta ccatagaaga ataaagtgaa agtctattga gttgttgaca ctcagcagat
     4621 ttttatatgt gaaagaaatt tcaaattttt tctaaaaata tcttgacaga ttaaattttc
     4681 aggagtagaa tatttactag ttaattaaat ggttaaggag ttgttcatga agaaacaaaa
     4741 tttattttta gtcctgttaa gtgtctttct tttgtgcttg ggggcttgtg gtcaaaagga
     4801 aagtcagaca ggaaagggga tgaaaattgt gaccagtttt tatcctatct acgctatggt
     4861 taaggaagta tctggtgact tgaatgatgt tcggatgatt cagtcaagta gtggtattca
     4921 ctcctttgaa ccttcggcaa atgatatcgc agccatctat gatgcagatg tctttgttta
     4981 ccattctcat acactcgaat cttgggcagg aagtctggat cccaatctaa aaaaatccaa
     5041 agtgaaggtc ttagaggctt ctgagggaat gaccttggaa cgtgtccctg gactagagga
     5101 tgtggaagca ggggatggag ttgatgaaaa aacgctctat gaccctcaca catggctaga
     5161 tcctgaaaaa gctggagaag aagcccaaat tatcgctgat aaactttcag aggtggatag
     5221 tgagcataaa gagacttatc aaaaaaatgc gcaagccttt atcaaaaaag ctcaggaatt
     5281 gactaagaaa ttccaaccaa aatttgaaaa agcgactcag aaaacatttg taacacaaca
     5341 tacagccttt tcttatctag cgaagagatt tgggcttaat caacttggta ttgcaggcat
     5401 ctctcctgaa caagaaccaa gtccacgaca attagcagaa attcaggaat ttgttaaaac
     5461 ctataaggtt aaaacgattt ttacagaaag taatgcttct tcaaaagtag ctgaaactct
     5521 tgtcaaatca acaggtgtgg gtcttaaaac tctgaatcct ttagagtcag acccacaaaa
     5581 tgacaagacc tatttagaaa atcttgaaga aaatatgagt attctagcag aagaattaaa
     5641 gtgaggaaag aatgaaaatc aataaaaaat atctagcagg ttcagtggca gtccttgccc
     5701 taagtgtttg ttcctatgag cttggacgtt accaagctgg tcaggataag aaagagtcta
     5761 atcgagttgc ttatatagat ggtgatcagg ctggtcaaaa ggcagaaaac ttgacaccag
     5821 atgaagtcag taagagggag gggatcaacg ccgaacaaat cgtcatcaag attacggatc
     5881 aaggttatgt gacctctcat ggagaccatt atcattacta taatggcaag gtcccttatg
     5941 atgccatcat cagtgaagag ctcctcatga aagatccgaa ttatcagttg aaggattcag
     6001 acattgtcaa tgaaatcaag ggtggttatg ttatcaaggt agatggaaaa tactatgttt
     6061 accttaagga tgcagctcat gcggataata ttcggacaaa agaagagatt aaacgtcaga
     6121 agcaggaaca cagtcataat cacgggggtg gttctaacga tcaagcagta gttgcagcca
     6181 gagcccaagg acgctataca acggatgatg gttatatctt caatgcatct gatatcatcg
     6241 aagatacggg cgatgcctat atcgttcctc atggcaatca cttccactat attcctaaga
     6301 gtgattt
//
LOCUS       DAVKWZ010000082         6190 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000082 DAVKWZ010000000
VERSION     DAVKWZ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6190
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.082"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(<1..677)
                     /locus_tag="VW854_001929"
     CDS             complement(<1..677)
                     /locus_tag="VW854_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263111.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAIVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFQ
                     SIFYFTR"
     gene            complement(687..1439)
                     /locus_tag="VW854_001930"
     CDS             complement(687..1439)
                     /locus_tag="VW854_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263112.1"
                     /translation="MKQFVQFYKKDFLAVLVYFILLLSCVLSSTVYLLRCHQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLDNGIIGLLPIFQYMV
                     NSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            complement(1439..2203)
                     /locus_tag="VW854_001931"
     CDS             complement(1439..2203)
                     /locus_tag="VW854_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263113.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKKYLAIVG
                     STVIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQLLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKLGAIFIPVAYMMVGGILWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(2299..2702)
                     /locus_tag="VW854_001932"
                     /pseudo
     CDS             complement(2299..2702)
                     /locus_tag="VW854_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2882..3514)
                     /locus_tag="VW854_001933"
     CDS             complement(2882..3514)
                     /locus_tag="VW854_001933"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEV0263114.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            complement(3548..4249)
                     /gene="pyrF"
                     /locus_tag="VW854_001934"
     CDS             complement(3548..4249)
                     /gene="pyrF"
                     /locus_tag="VW854_001934"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEV0263115.1"
                     /translation="MREHRPVIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKILSQLGVDMTNIHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            4570..4662
                     /locus_tag="VW854_001935"
                     /pseudo
     CDS             4570..4662
                     /locus_tag="VW854_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516271.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(4984..5697)
                     /locus_tag="VW854_001936"
     CDS             complement(4984..5697)
                     /locus_tag="VW854_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0263116.1"
                     /translation="MLKRFLALVWLRCQIILSNKSILLQVLVPFAFTYFYKYLMETQG
                     KVNDQQALVLLMMCLPFSLALAVGSPITIILSEEKEKYNLQTLLLSGVKGSEYILSTM
                     FLPFLLTFVIMGTTPLILGVTIVHTFNYITIVLLTSLSIILFYLLIGLTAKSQVVAQV
                     ISLPAMILVAFLPMLSGLDKTVAKITDYSFMGLFTKFFTKWEGFSWNKTLIPNLTLLI
                     WIVLLLTLITITIRKKKIS"
     gene            complement(5690..>6190)
                     /locus_tag="VW854_001937"
     CDS             complement(5690..>6190)
                     /locus_tag="VW854_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0263117.1"
                     /translation="LLKRVGLYDSRKMVAGKLSTGMRQRMLLARALINKPAVLFLDEP
                     TSGLDPTTSRTIHELILELKTAGTTIFLTTHDMNEASLLCDYVALLNKGKLVEQGAPS
                     ELIQRYNKDKKIKVTDYNGNQITFDFTSLEQVSQADLENIFSIHSCEPTLEDIFITLT
                     GGKLNA"
BASE COUNT         2180 a         1229 c          982 g         1799 t
ORIGIN      
        1 tgacgagtga aatagaaaat gctttgaaaa agaaagacta aaaatagcaa ctgaagaaat
       61 aaatctcgtc ctatatctcc attccctcga ttcaaaatat aaaatagata attagttcga
      121 tttcctacaa atagaccacc aaacacaatc atgagtaaaa agaaactaac gcaagcaaag
      181 ttcatttgta cattcgctcc tagtgcgtat aacataaagc aaaacaagac taacacacaa
      241 gcattcaaag aatcaactat aaacaaaact cctatatccc agtttggtaa ctgtaacata
      301 aattgaatca ctagaacttt taccatgatt aaaatgagtc cataaagaaa acaggtccaa
      361 atctgatacc taatccagag cttaatagaa gaaaatcgaa tcacttccat aaagaaatcg
      421 gatgctcttc tacgatatac aattgctaga aaaaatgtat gaataataaa ccaatgaagc
      481 aaccatatag ataagactaa aaaagagttt tgactcagat taggcccttt taaaatttct
      541 ataacaagtc tatcatttcc ttctaaccaa taccataatc ctaaacaaat caatatccaa
      601 aaaagaacat aaccccaaaa gtacttggta tagattatct gattcaacct ccacatgtga
      661 ctacttaact ttcccatttc caacttttac actctcctcc agtttctatg aacagaaaat
      721 acagtcaatg gaattatgat agatagtaat gtaagccaat aaatcaatgc atacggattg
      781 gaatttacca tatactgaaa aattggcaat aatccaatta tcccattgtc caactgataa
      841 atccagaata aggattctac aaataaaaaa gtaaacagaa atactaagct catgtttgat
      901 tttttaaacc aagaaaaact gctaatagta agtaaggata aaaagaaata gtatgctata
      961 taagagaaaa aaaatcctat aaagaaacta gcattgtttg caatacttat accataaaat
     1021 tctcttaaaa aatcaacctc ctttatctcc aaagagaagc taaaaccatt actaaatgca
     1081 attagaaaaa tcaataaaaa taaagtcgcc gtccaaatcc ccgtactaag agctgctaat
     1141 ttgaaactaa aactggtaaa gtgcttaatt gatttcagac gaatgcgaca ctccaaccta
     1201 ttaaaatagt tattcatcaa ataaaaaaag aacaatatat atgtgaacgg aaagcaatat
     1261 actccagtcg tcatatcttg aagtaaaact aagatccatt ctaatacatt tggatggatt
     1321 gaatattggt gacagcgcaa taaatatact gtactagata aaacacagga tagcagtaat
     1381 ataaaataaa ccaatactgc taagaaatct tttttataaa attgaacaaa ttgtttcatt
     1441 atacatagtc ctctgaatgt agaaaaaatg taccataaac aaccaaacaa ctaacaaata
     1501 aaataaaagc aagatgccca ctaactaagg aaagactgat atctttctga tatcccaaag
     1561 ctagcgttgt cacaggttct aagtaagata gccctaaaat agcccataaa ataccaccaa
     1621 ccatcatata ggcaactggg atgaaaatag ctcctaattt tttcttcact agcaaagcac
     1681 tagctagtcc aaaaatagag aacacagcgc cccaaactcc ataccagaga gtcgtcacaa
     1741 gactatagag caactgatta gaatcaaata attcttttaa ggcaccacta taatctccaa
     1801 tataaatttc ctgataagga gtcactaaaa gattaattcc taataataat aaatagggga
     1861 gaaaaaagac tagaaaagaa gaaataatta cagtactacc cacaatagcc agatacttct
     1921 ttttagaaat tcgacacaat tgtgctgtta gaaaatgact ctcagcatcc tctattatct
     1981 gactagaata gggcagtgta cagataagtg cagctactag gctaatcggt gaaaatcctc
     2041 gaatagaaga agcggcaaaa aaaatcgata aaccttcaat tttataaata ccattgaaag
     2101 caaggaaatt tcctaaactc atgcaaagaa gggtcaaaaa taaagacata taaaaccgag
     2161 gtgattgaac gactcgtaca agattacaaa tgaaaaattc catcccaact cctccttata
     2221 ataaaaatag ggtgtagcat tcttttttca tgctacaccc acaatcaacc atctttaagg
     2281 cttactctga caagtaagtt aataagaatc tgaactccaa gaacctgaag tatgaattct
     2341 tacatgattt ccaaattgtg gcgccatagc taatctagta ccagaaccaa tataattgtc
     2401 accacctcca ttatagtaca tgacaatcct agagccagac cccaatgaat ataccggggt
     2461 aatatctgac ccactatagg cgctacgaat agaggtattt aaccttttac cgccaccagt
     2521 gctgtcacta ttattaattc cagcagaggc gttttctttc tcaagctcac tagttgtatc
     2581 cgaaccccaa cgcggcaacg ttaaatcata atttccaatc tgtctagcta aaactgtagg
     2641 gagaaacaaa ctagatgcaa ttgtaactac gcaaaaaagg aatatatttt tcttcttttc
     2701 atgacatcgt cctcttaaga agtgataaac aagatagttg attgatcaat ttgatacata
     2761 ttacattttc attatatcaa taaaagaaag cgttgtcaat tattttattt aaaatattgt
     2821 catattttaa ataaaatggt aaaagagact cggtagcctc gtctctcttt atcttactga
     2881 cctaaccttc ttgccaattt tcttggtctt ctttaaagcg ttttagaaga tcaaggccct
     2941 ctggcgtgat gtaaccttct tcttgggcta gatggataag ctcgctatag tttgaaagcg
     3001 tcacaagttt aacaccagca tctgcaaagt tcttatctgc ttttggcaat tggtagctga
     3061 aaatcgctac aactccaagc acatctgctc cttctcgctt ggctgctgct acagcttcaa
     3121 gaactgaacc acccgttgaa ataaggtctt caaccactac cattttttga ccttgagcta
     3181 cgcgaccttc gatttgatta ccagctccat ggtcttttgg tttgctacgg atgtaggcaa
     3241 aaggcaagtc catcttatca gcaataatgg ctccgtgtgg aatccctgct gttgcagttc
     3301 ctgcaatcac ttctacttca ggaaaggctt ctttgatagc ttccacaaaa ccattttcaa
     3361 ttagggtacg agtttctgga taggctagtg tcacacgatt atcagtgtaa atcggtgact
     3421 tgataccaga tgcccaagtg aagggttcct ctggtttgag gtaaacggct tggattttca
     3481 agaggtggct agcgatatct ttagcaagtg tcatggtatt ctccttttat ttttctaatc
     3541 tagttcttta attccagtcc tgtgtccatt catccttgat ggcatgataa gctgcaacag
     3601 gatcctcagc ttgggtaatg ggacgtccca ctacgatata gtcactgccg atttgatagg
     3661 catcagcagg tgtcatgact cgtttttgat ctccaactgc aacaccagct ggacgaatcc
     3721 ctggtgtcag acagataaaa tctggattgg tagcctgctt gatgacttgt acttcctgag
     3781 ccgagcaaac aacaccatcc aagccagctt cagctgtctt cttggcatag tgaatcacag
     3841 actcttgcag actggtttgg atattttgaa actcctgcat ctgagcttct gacgttgatg
     3901 tgagctgcgt tacagcgatc aatttggctt gacttccaag accttcacgc gcagccttca
     3961 tcatctctac accaccagcc gcatgaatat tggtcatatc cacaccaagc tgagatagga
     4021 tcttcattgc tgacttgact gtattaggaa tgtcatgaag tttgagatcc aaaaagacac
     4081 tatgacccaa accttttaag taggacacaa tctcaggccc cgctgcgtaa taaagctcca
     4141 tccctacctt gagataaagg ctttcttctg ctgggaaaag agctaaaaat tccttgaccg
     4201 cctcaaaact aggaaaatca agagcaatga ctggacgatg ttctcgcata ggtttctcct
     4261 tttaccttta ctagtgagag agtctggcca acagaagcca cgaaaaaagg aacctgccaa
     4321 cagcaaggtt ccacgaaaaa tgggtagtct ccaccctttc accgtctaac cttagctgcc
     4381 tctctggact gctttaaagg ttttctatta ttctattatt tctactagca cttgtcaagt
     4441 aaaaacttag tcacgtatgt gttgactgct attgaacacc tcacaagact ctttccaaaa
     4501 gaatagttaa agactttcac ttaggacaga gaaaaagagt ttgcttgtta ccctttaatt
     4561 gaacaaatgg gaattctgtt ttattttgcg gatacctatt catcttggca aaggggaagt
     4621 aatgagaatg cgaatggact tcttcgtgag tattttctta aaccgatttg gcaacaatta
     4681 ccgaaaaaat ccttaatggt gctttgctag cttattaatc accgtcccca aaaatacttg
     4741 gattacaaga caggttttga agcacttata gaggagattt aaaaatgttg cattttttat
     4801 tgcaatttat cagttatctt atcacccaaa acttctgcaa ttagtatgag cactattgtt
     4861 atttttcttt tttcgtacac tcaaatcaaa atatagcaat aagactattc tgtagaattg
     4921 ctgtcaaaaa gatcagttca tttttccttc ccacttgtgt ttataatcat taaaataact
     4981 caattaagaa attttctttt tcctaatagt tatcgtaatt aaagttaata gaagaacaat
     5041 ccaaataagt agtgttagat taggaattag agttttattc catgaaaatc cctcccattt
     5101 tgtgaaaaac ttagtaaaca gtcccataaa actataatct gttatcttcg caactgtctt
     5161 atccaaacca gatagcatcg gtaagaaagc aactaaaatc atagcaggaa gactgataac
     5221 ctgagctact acttggctct tcgcggttaa acctatcaat aaatagaata aaatgatgga
     5281 taaagaggtt agaagaacga ttgtaatata attaaaagta tgtacaattg taactcctaa
     5341 aataagagga gtagttccca taatcacaaa agttagcaaa aaaggaagaa acatcgttga
     5401 taaaatgtat tcggatcctt taacaccact caacagaaga gtttgtaaat tgtacttttc
     5461 tttttcttca gacaagataa tagttatagg acttccaaca gccaaagcca aagaaaaagg
     5521 taaacacatc atcaaaagaa ctaatgcctg ttgatcgttg accttcccct gtgtttccat
     5581 aagatattta taaaaatatg tgaaagcaaa aggcactaaa acttgcaata aaatactctt
     5641 attggaaagg atgatttgac aacgcaacca taccaaagcc agaaaccgtt taagcattta
     5701 gctttcctcc tgttaatgtg ataaaaatat cttctaaagt aggctcacat gaatgaattg
     5761 aaaaaatatt ttccagatca gcctgagata cctgttctag tgatgtaaaa tcaaaagtta
     5821 tctgattccc attataatct gtaaccttaa tctttttatc tttattatat ctttgaatga
     5881 gttcagaagg agctccttgc tcaactaatt tccctttatt taataaggca acataatcac
     5941 ataaaagact tgcttcattc atatcatgag tcgttagaaa aatcgttgtc cctgctgttt
     6001 tcaattctaa aattaactca tgaattgttc gagaagttgt gggatctaga cctgaggtcg
     6061 gttcatccag aaagagtaca gcgggtttgt tgataagagc tcgtgctaaa agcattcgtt
     6121 gcctcattcc agtggataat tttcctgcta ccatcttgcg actatcatat aatcctactc
     6181 gctttaacaa
//
LOCUS       DAVKWZ010000083         6141 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000083 DAVKWZ010000000
VERSION     DAVKWZ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.083"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            185..1954
                     /locus_tag="VW854_001938"
     CDS             185..1954
                     /locus_tag="VW854_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEV0263118.1"
                     /translation="MEEKVSLKVRVQKLGTSLSNMVMPNIGAFIAWGVLTALFIADGY
                     LPNEQLATVVGPMLTYLLPILIGYTGGYMIHGQRGAVVGAIATVGAITGSSVPMFIGA
                     MVMGPLGGWTIKKFDEKFQEKIRPGFEMLVNNFSAGLVGFALLLLAFYAIGPVVSTLT
                     GAVGNGVEAIVNARLLPMANIIIEPAKVLFLNNALNHGIFTPLGVEQVVQAGKSILFL
                     LEANPGPGLGILLAYAVFGKGSAKSSSWGAMVIHFFGGIHEIYFPYVMMKPTLFLAAM
                     AGGISGTFTFQLLDAGLKSPASPGSIIAIMATAPKGVWPHINVLLGVLVAAVVSFLVA
                     ALILHADKSTEDSLEAAQAATQAAKAQSKGQLVSTSVDAVVSTDSVEKIIFACDAGMG
                     SSAMGASILRDKVKKAGLEIPVSNQAISNLLDTPKTLIVTQEELTPRAKDKSPSAIHV
                     SVDNFLASPRYDEIVASLTGASPIAEIEGDIPTSAPVDSQEIDLNHIDAVVVAYGKAQ
                     GTATMGCETIRAIFRNKNIRIPVSTAKISELGEFNSKNIMIVTTISLQAEVQQAAPNS
                     QFLIVDSLVTTPEYDKMAARMYK"
     gene            1978..3933
                     /locus_tag="VW854_001939"
     CDS             1978..3933
                     /locus_tag="VW854_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV0263119.1"
                     /translation="MLLTKREEQLLKAFLHVGKLSMQDMTEILQVSSRTIYRTLSDLT
                     DSMEQYGIEITKHGKYYILTGELDDLPTELEVLVEYSPQERQELITYRLLTESGFVTN
                     EALQECTKVSNVTIIQDISDIDKRLLDFDLKIERQKGYRISGDSVGKRRFLAILLTNC
                     ISVADFSTGNFGSFDILEADRTRLASQIVNKQLSGFPDMDARMKMFFAILLSLIGQEQ
                     NIENSPNTSKQALEISQKIFQTYSKQTAQFYSIQEIIYFASILDELIIKRQDNPLFTE
                     KFDGEFFYNISNLIDTVSMYTKIDFFKDKILFNFLFHHIRLSLGVPILFQGENLPESV
                     QILVERNKFLYTVISLLVNDIFPKYLHTEYEYGMIALHFISSLGRSPEIYPVRVLLLT
                     DERRVTRDLLVSKIKSVAPFVELIDIQSLVDYHSIDLSQYDYILSTKPLTNQEIDVIS
                     SFPTVKELLELQERLQYVQAHRTIVARDAIAPEKSYDLQDYLISSSQLLSQFELVQLE
                     NNQSFEHTVEQIIQYQKNVSDRDYLTRKLLSHFQNSPMAIPNTGLVLLHSQSSKVTTN
                     SFTMFELKLPISALSMKREEEEVKRCLLMLMSKEASEEARDLMTAISQSIIENHLYTE
                     IYKTGNQSIIYQMLNTIFNEKIKKLEN"
     gene            3935..4372
                     /locus_tag="VW854_001940"
     CDS             3935..4372
                     /locus_tag="VW854_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV0263120.1"
                     /translation="MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNEDYIEAMIE
                     RDKELSVYMGNFIAIPHGTDAAKKDVLKSGITVVQVPRGVDFGNVSNPQVATVLFGIA
                     GIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE"
     gene            4434..5570
                     /locus_tag="VW854_001941"
     CDS             4434..5570
                     /locus_tag="VW854_001941"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEV0263121.1"
                     /translation="MKHSVHFGAGNIGRGFIGEILFKNGFHIDFVDVNNQIIHALNEK
                     GKYEIEIAQKGQSRIEVTNVAGINSKEHPEQVIEAIQRTDIITTAIGPNILPFIAELL
                     AKGIEARRVAGNTQALDVMACENMIGGSQFLYQEVKKYLSPEGLTFADNYIGFPNAAV
                     DRIVPAQSHEDSLFVVVEPFNEWVVETKRLKNPDLRLEDVHYEEDLEPFIERKLFSVN
                     SGHATSAYIGAHYGAKTILEALQNPNIKSRIESVLAEIRSLLIAKWNFDKKELENYHK
                     VIIERFENPFIVDEVSRVARTPIRKLGYNERFIRPIRELKELSLSYKNLLKTVGYAFD
                     YRDVNDEESIRLGELLAKQSVKDVVIQVTGLDDQELIEQIVEYI"
BASE COUNT         1884 a         1032 c         1253 g         1972 t
ORIGIN      
        1 ttttttctgt ctaacttttg gggtgtagtt catgtacacc tgatatgatg cgttttataa
       61 tttttaagcc tttttgccca gcctcgtcaa aagtaatgtt ttgacacaaa atctgtgaca
      121 aaactttagt tttaaaggtt tttaactttg tatatactag ttttaagaaa aggaggatga
      181 tctaatggaa gaaaaagtat cattgaaagt cagggttcaa aaactaggga catcgctttc
      241 aaatatggtt atgcccaata ttggagcatt tattgcttgg ggagtattga ctgccctctt
      301 tatcgctgat ggctatctgc caaatgaaca gttagctact gttgttggtc ctatgttaac
      361 gtatttattg ccaatcctga ttggttacac aggtggatat atgatccatg gccaacgtgg
      421 tgccgttgta ggagctattg ctactgttgg tgcaatcaca ggttctagtg ttcctatgtt
      481 tatcggagct atggtaatgg gcccactggg aggatggact atcaagaaat ttgatgagaa
      541 gttccaagaa aaaattcgtc ccggatttga aatgttagtt aataacttct cagctggtct
      601 cgttggtttt gcattattgc ttttggcttt ctacgcaatc ggtccagtcg tatcgactct
      661 tactggagct gttgggaatg gtgttgaggc tattgtcaat gctcgcctcc ttcctatggc
      721 taatattatc atcgaaccgg ctaaagtcct tttcctcaat aatgccctca atcatggcat
      781 ttttactcct ctgggagtag aacaggtagt tcaagctggt aagtcaattc tcttcctatt
      841 ggaagctaat cctggaccag gcctgggaat tctattagct tatgctgtat tcggtaaagg
      901 ttctgctaaa tcttcttctt ggggggcaat ggttattcat ttcttcggag ggattcatga
      961 aatttacttt ccttatgtta tgatgaagcc tactctattt ttagctgcta tggcgggagg
     1021 tatctctgga acttttactt ttcaactctt agacgctggt cttaaatctc cagcttcacc
     1081 aggttctatt attgcgatta tggctacggc gccaaaaggt gtttggcccc atataaatgt
     1141 tcttctaggt gttttagtag cagcagttgt ttctttcctt gtagcagccc ttattcttca
     1201 tgcagacaag tcaactgagg attcgctcga agctgctcag gcggctaccc aagcagctaa
     1261 ggctcagtct aaaggtcagt tagtatcaac ttctgttgat gcagttgttt cgacagactc
     1321 agtggaaaaa atcattttcg cctgcgatgc tggtatggga agctctgcta tgggagctag
     1381 tattcttcga gataaggtta aaaaagcagg tctagagatt ccagtatcta atcaggcaat
     1441 ctcaaatttg cttgatacac caaaaacatt aattgttact caggaagaac tgacaccaag
     1501 agctaaagac aagagtccaa gtgctattca tgtttctgtt gataatttct tagcgtcccc
     1561 tcgttatgat gaaattgtag cttcattaac aggagcttct ccaatagcag aaattgaagg
     1621 agatatacca acttcagcac cagtagatag tcaggaaatt gaccttaacc atattgatgc
     1681 tgtagtagtt gcttatggta aagcacaggg aactgcaact atgggctgtg aaacgattcg
     1741 ggctatcttt agaaacaaga atattcgtat tccagtttct actgccaaaa tttcagaatt
     1801 aggtgaattt aattctaaaa acataatgat tgtaacaact atttctttac aggcagaagt
     1861 gcagcaagca gcaccgaatt ctcaatttct tattgtggat agtttagtaa caacaccaga
     1921 atatgacaaa atggctgcta gaatgtacaa atagaactag aggtttctaa attacgaatg
     1981 ctattaacca aacgagaaga acaattattg aaggctttcc tacatgtagg gaagctttca
     2041 atgcaagata tgactgaaat cttacaggtt tcatctagaa caatttatcg aactttatca
     2101 gatttgacag atagcatgga gcaatatgga atcgaaataa cgaagcatgg gaaatactat
     2161 attttgactg gagagttgga tgatttgccg acagaacttg aagtgttagt tgagtatagt
     2221 ccccaagaaa gacaagagtt gattacctat cgccttctga ctgagagcgg ttttgtcacc
     2281 aatgaagcat tgcaagagtg cacgaaagtc agtaatgtaa ctattattca ggatatttca
     2341 gatattgata agcgtctttt agactttgat ctgaaaattg aacgacaaaa aggttatcgg
     2401 atttctggtg attcagttgg taagagaaga tttttggcta ttttactgac aaactgtatc
     2461 tcagtagcag atttttcaac cggtaatttt gggagctttg atattttaga agcagataga
     2521 actaggctgg ccagtcagat tgttaataag caactgtcag gttttccaga tatggatgct
     2581 aggatgaaga tgttttttgc gatcttgtta tctcttatag gtcaggagca aaacattgaa
     2641 aattcaccta atactagtaa gcaggctttg gaaatttctc aaaaaatttt tcaaacttac
     2701 tctaagcaga ctgcacaatt ttatagtatt caggaaatta tctattttgc gagcatcttg
     2761 gatgaattaa tcattaaacg tcaggacaat ccgctcttta cggagaaatt tgatggtgaa
     2821 tttttctaca atatttcaaa tctgattgat acggtttcca tgtataccaa gattgacttt
     2881 tttaaggaca agattttatt caattttctt ttccatcata ttcggctcag tttaggcgtc
     2941 cctatccttt ttcagggtga aaatttgcca gaatctgtcc agattttagt tgaaaggaat
     3001 aaatttcttt atacagtcat cagtctttta gtgaatgata tttttccgaa atatcttcat
     3061 acagagtatg agtatggcat gattgcccta cattttatct ctagcttagg ccgtagtcca
     3121 gagatttatc cagtccgtgt tttgctttta acggatgaac gtcgggtcac tagagattta
     3181 ttagtcagta aaattaagag tgttgctcct tttgtagagt tgatagatat tcagtctcta
     3241 gtagattacc acagtattga tctcagtcag tatgattata ttttatctac caagccgctg
     3301 actaatcagg aaatcgatgt aatttctagt tttccaaccg tcaaagaatt gcttgaatta
     3361 caggaacgac ttcagtatgt acaggcacat cgtacaattg tcgcgcgtga tgctatcgct
     3421 ccagagaaaa gttatgactt gcaagattat ttaatatcta gtagtcagct tttgagtcaa
     3481 tttgagttgg ttcaattgga gaataatcaa tcatttgagc acacggtaga acaaatcatc
     3541 caatatcaga agaatgtgag tgacagagat tacctaacaa gaaaattgtt atctcacttc
     3601 cagaatagtc ctatggctat tcctaatact ggtctggtgc ttttacatag tcagtctagc
     3661 aaagtaacaa caaatagttt tactatgttt gaactcaaac tacctatctc cgcattgtca
     3721 atgaaacgag aggaagaaga ggtcaaaagg tgtctgctaa tgctaatgtc taaagaagct
     3781 agcgaggaag ctagagattt aatgacagct attagtcagt cgattattga aaatcatctt
     3841 tatacagaga tttacaagac gggaaatcaa tccattattt atcagatgct aaatactatt
     3901 tttaacgaaa aaattaagaa attggagaac taatatgaaa cttgaaaaac atttgattaa
     3961 gcttaataaa caattttcta acaaggagga agctatttgt tattgtgggc aagttcttta
     4021 tgagggtgga tatgttaatg aagactatat tgaagccatg attgagcgag ataaagagct
     4081 atctgtttac atgggtaact ttatcgccat accgcatgga acagatgcag caaaaaagga
     4141 tgtcctcaag tctggtatta cagtcgttca agtccctaga ggggttgatt ttgggaatgt
     4201 atctaaccct caagtggcaa cggttctttt tggcattgct ggtattggta atgaacactt
     4261 agaaattatt cagaaaattt ctatcttctg tgcagatgtg gataatgttc ttaaactagc
     4321 agatgctcag tcaaaagagg aagtattgcg cttatttgat gctgttgaat aattgaattt
     4381 cgtcatttgt catctagtat atatgtccct caaataggaa aaggagaaat tgaatgaaac
     4441 attctgttca ttttggtgcc ggtaatatcg gtcgtggttt tataggtgaa attctattta
     4501 aaaatggttt ccatattgat tttgtggatg tcaataatca gataattcat gctctaaatg
     4561 aaaagggcaa gtatgaaatt gaaattgcac agaaaggaca gtctcgtata gaagtaacta
     4621 atgtggctgg aattaatagc aaagaacatc ctgagcaagt cattgaagcg attcaaagga
     4681 cggatattat tactactgca atcggaccta atatactccc ttttatcgcc gaacttctag
     4741 ccaaaggaat cgaagctcgc cgagttgcag gaaatacaca ggcattggat gttatggcct
     4801 gtgaaaatat gattggcggg tctcaatttc tttatcaaga agtcaagaaa tatttaagtc
     4861 cggaaggttt gacatttgct gataactaca taggttttcc aaatgctgca gtagacagga
     4921 ttgttccagc acaaagtcac gaagattccc tttttgttgt ggtcgagccc tttaatgaat
     4981 gggtcgtgga aaccaagcgt cttaaaaatc cagatttacg tctagaagat gtgcattatg
     5041 aagaagattt agaacccttt attgagcgaa aacttttttc agtcaattct ggacatgcaa
     5101 cttcagctta cattggtgcg cattatggtg ccaagacaat tttggaagct cttcaaaatc
     5161 ctaatatcaa atctcggatt gaatctgtat tagctgaaat tcggagtctc ttgattgcca
     5221 aatggaactt tgataaaaaa gaattggaga attatcacaa agtcattata gaacgatttg
     5281 aaaacccttt catagtggac gaggttagtc gcgtagctcg tactccaatc cgaaaattag
     5341 gctataatga acgattcatc cggccgatac gtgaattgaa agaactcagt ttgtcatata
     5401 aaaacctact taaaacagtt ggctatgcct ttgactatcg cgatgtaaat gatgaagaaa
     5461 gtattcgatt aggtgaattg ttggctaaac aatcagtcaa agatgttgtt atacaagtta
     5521 caggtttaga tgaccaagaa ttgattgagc aaattgtaga gtatatttaa tctttttcga
     5581 aagtctcttc aaatcaggtt agcatccatc tgcaacctca aaacagtatt ttgagctgac
     5641 ttcgtcagtt ctatctacaa cctcaaagca gtgctttgag ctgactccgt cagtcttatc
     5701 tgcaacctca aaacactgtt tgagttatct gcggtaatct ttctagcttg tctttgattt
     5761 ttgttgttat ttataaggta aaagaagctg gacaaaaagt cttcaaaatc ggaaaaaggc
     5821 agcctatcgg gtgttcaaaa atcttgatag gatgtccttt attatggaaa gccttattgg
     5881 attttctcct cagattgagt ttttgatcag ctttatgaga taggtcttgc tagagatgta
     5941 gcccatcatg ttatttttat ggacagtggg aaaattgttg aaaaaaataa tgcccatcaa
     6001 ttctttagtc gtccaagaga agaacgaacc aagcaatttt ggaacgaatt ctttcgaatg
     6061 cgatctatat agtaaaatga aacaagaaca ggacaaatcg atcaggacag tcaaatcgat
     6121 ttctaaaaat gttttagaag t
//
LOCUS       DAVKWZ010000084         5942 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000084 DAVKWZ010000000
VERSION     DAVKWZ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5942
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.084"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(24..575)
                     /locus_tag="VW854_001942"
     CDS             complement(24..575)
                     /locus_tag="VW854_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0263122.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDVEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGHGMMTEALKAVLDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            complement(822..1646)
                     /gene="nadE"
                     /locus_tag="VW854_001943"
     CDS             complement(822..1646)
                     /gene="nadE"
                     /locus_tag="VW854_001943"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEV0263123.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            complement(1643..3103)
                     /locus_tag="VW854_001944"
     CDS             complement(1643..3103)
                     /locus_tag="VW854_001944"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEV0263124.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            complement(3219..3707)
                     /locus_tag="VW854_001945"
     CDS             complement(3219..3707)
                     /locus_tag="VW854_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263125.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            complement(3697..3906)
                     /locus_tag="VW854_001946"
     CDS             complement(3697..3906)
                     /locus_tag="VW854_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0263126.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            complement(4291..5019)
                     /locus_tag="VW854_001947"
     CDS             complement(4291..5019)
                     /locus_tag="VW854_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0263127.1"
                     /translation="MKLNKFNFLKENIRNLYSSGVIYLSLLISFIPPILLTFFILKTQ
                     GTSLGIKHISNFYAMLGMLMAVIHANRVISRDFSHNTVSLFYNQQKNRMIYVLSNFLY
                     AISVSIIYALNGIVLLVIVSKLGIPGDLGLDFIVAIVVNTILLVLFYFLLSYIFYLYK
                     LKSGLVFGILVALLLFIPNILNTMMMNTSNDLFIKAIELLPFYSLPVFVASNTMSISQ
                     YLVLITTIILLYFFTLKKSKKYSF"
     gene            complement(5035..5826)
                     /locus_tag="VW854_001948"
     CDS             complement(5035..5826)
                     /locus_tag="VW854_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0263128.1"
                     /translation="MNFALNKGEIVGLAGRNGVGKSTLMKILVQNNQPTSGNIISSDN
                     VGYLIEEPKLFLSKTGLENLKYLSNLYGVDYNQEIFRSLIQELDLTQSINKKVKTYSL
                     GTKQKLALLLTLVTEPDILILDEPTNGLDIESSQIVLSVLKNLALHENVGILISSHKL
                     EDIEEICERVLFLENGLLTFQKVGKDSHNFLFEIAFSSATDRDIFITKQEFGDIVQEE
                     GLSITMSGNIQSSELFKFFNENSIKVVDFETKKETLKDIYLNRSK"
BASE COUNT         1820 a         1274 c         1014 g         1834 t
ORIGIN      
        1 tcgatagaaa cagaaaatag aattcaacat tcttccctac ttataccata ataaataaga
       61 tccgcaagat agccttttct ctctacacca ttaacaatgg tttgtagata ggacattcca
      121 gccttttcca tgacacgacc tgaagctggg ttgagactgg cataacgtgc tctgaccttt
      181 tgaaaacctg cttgagtaaa acaaaagtcc aagacagctt tcaaggcctc tgtcatcata
      241 ccatgtcccc agtaagcctt gcctagcaca tagccgattt cacagcttaa atcagcctcg
      301 tctatcttaa cgatgctgat atctcctatc acttgctctg ggttttcttt tagacaaatg
      361 gcccatttat aatagttgag attagtatag gaagcaaccc aattgcgaat cgagtttcga
      421 gtgatttcga catcaggatg gggatcccag gtaacatagg tcagattctc agcggatgaa
      481 gcccaatttt gaaacatggc ttctgcatca ctctccacaa atcttcttaa aatcaaacgt
      541 tctgtctgta atatttgcgt accgatagat ttcatgatgc tacctcactt ccttatttct
      601 atgctcgtag taaaaagact caactcagta acagctagaa aacgagtttg tcttctaatt
      661 tcttgacgca gttgctgcat ggtttgaaat gcgctctagc aatctttttc aaacctagtc
      721 atcgttgcag gggtaagacg atgaaatcga actcttcgag ttaccgaatc tgcctcattt
      781 tcttatttct aaactcgggg ttcaagatcc cccggacctt tttactccca aaagtcatca
      841 aatacggtga tgggtaagtg gcgtttgtgt tggcctttgt gccaccagtt ttcaatggtc
      901 gcttgagctt ctgggctgat tgttttgcct tctaggtaat cgtcaatctc tgcataggtg
      961 actccaagtg cgacttcgtc agctaggcct ggtttgtctt cttctaggtc tgccgttggg
     1021 attttttcat aaagggctgg ctctgcgcca agtttctgca agagttgttt tccttggcgt
     1081 ttattgaggc ggtaaagagg gagaatatcc gcaccgccgt caccaaactt ggtaaagaaa
     1141 cctgtgatat tttccgcggc gtggtctgtt ccaatgaccg ctccgctatg ggaaccagca
     1201 agggcatact gagcaatcat acggcaacgt gccttgatat tccccttgtt gaagtctgaa
     1261 acaggactac ctgtcgcttc aactgcagct gtcatggcat cagctgattc cttgatattc
     1321 acaaccaagc tgacatctgg ctggatgaag gctagggctt tttgagcatc tgcttcatca
     1381 gcttgcactc cgtatggcag gcggacagcg ataaatttgt agctatcgtc tcccgtttca
     1441 gctcgcagtt cttccatagc taattgcgcc aaacgtcctg ccaaggttga atcttgtccc
     1501 ccagaaatcc ctagtacaaa ggtttttagg aagggatgtt ttttcagata tctttttaag
     1561 aaatcaatag aacgacggat ttcttcctgg gcatcaatca ctggtttgac acccagctct
     1621 tggataatcg tttcttgcaa actcattctt cttctccttc accaagggct tccttgcgca
     1681 tcttatcaat caagtccatc ttatcttgcc atacatcacg cgccaaatcc actggatagt
     1741 gctgcggatt gagcacacgc ttatactcat cccacaactt gtcaaattcc ttacgggcat
     1801 aatcctgaat gtcagtcaaa ctaggcaagt tgtaaactaa tataccttct ttgaagatat
     1861 ccaccaagag aggaacggca tcaaaattac gaaccgtctt cttgatgtat gtataggtcg
     1921 gatggaacat cttgatttct gtcatgtcgc taatatccac accatcataa gtgatgtagt
     1981 caccttctga cttgcctttt tcacgactgg taatgcgcca cacctgcttc ttacctggcg
     2041 tcgacacttt ttccgcatta ttagacagct taatcgtatt gcgcatctga ccagtttcat
     2101 cttcgattgc aacaatcttg taaaccgccc caagagctgg ctggtcatag gctgtaatca
     2161 gcttggtacc cacgccccag acatcaatct tggccttttg catcttgagg ttgaggatag
     2221 tattttcgtc caaatcatta gaagcataaa tcttagcctc tgtaaatcca gcctcgtcca
     2281 gttgctgacg gactttctta gaaatgtagg caatatcccc agagtcaatc cgcacaccca
     2341 taaagttaat ctgatcaccc agctcacgcg ccacctgaat ggcagctggt acaccgatgc
     2401 gaagggtgtc ataggtatcc acaagaaaga cacaattttt gtgggtcgca gcgtaagcct
     2461 tgaaagcttc atagtcattg ccataaacct gtaccaaggc atgggcatgg gttcccaaaa
     2521 caggaatgtc aaagagctta cccgcacgca cgttgctggt tccattggcg ccaccaatca
     2581 cagctgcgcg tgttccccag atggccgcat ccatttcttg agcccgacgt gtcccaaact
     2641 ccatcaaggg ttcatcttcg ataaccgaac gaatacgagc tgccttcgtc gccaccaagg
     2701 tctggtagtt gacgatgttc aaaagagccg tttcgaccaa ctgacattgg gctagaggtc
     2761 cttccacctg cacaatcggt tcattagcaa aaaccaaatc cccttcttgg gcagaacgaa
     2821 cggtcaactc caacttgaaa ttgcgaaggt aatccaagaa cgccccatga taaccaagcg
     2881 actccaaata ggctatatca ctatctgaaa aacgcaagtc ttcaagatag ttcacaattc
     2941 tttccaaacc tgcaaaaacc gcatagccgt tcttaaaagg ctgttggcgg aaatacacct
     3001 caaagaccgc cttcttattg tgaatccctt ggtcaaagta aacctgcatc atgttgatct
     3061 ggtacaagtc cgtgtgcaat gtcaaactat catctggata catacttttc ctacttcctt
     3121 agctagaaac ccatgaaaat tttcaagaac tttcatgtat tccaataaat tagtactatt
     3181 atatcacatt ttagctggat tgagaaaaga gtaacaagct attctccact ctccaattca
     3241 tccatatctt gttcaaattt tttctgagcc cattcgccat agctcttaag accaagattg
     3301 ccaataaaga cccacggaag gtaaatgaca taagtaatga cccaagcaga caggtattta
     3361 aaattcaaag gattgtgctg ataaatttct atgttgaatt gataattctg caacatcaaa
     3421 agagccgtaa tagccaaggt taggaaaaaa caacccaaaa tcgtaaaatg aaaacgacta
     3481 tagtaggtca ctcccagata acgggcacga ttgaaaaagt aaaatgtccc tatgatgata
     3541 acgattagca gcatattaga attaaaaagg cttggtgcta atactgaaat gatataagat
     3601 aggagcgaca aagcaatgca gatatagaaa ctttcagagc ccgctttatt gaacagttgt
     3661 tcttctcttt cgtctagtaa ttgataataa taaaatctat ttttcatctt cttcctccca
     3721 aaatagttgg tctagggttt tccctaaaca tctgcaaata gactggcaga gcgagagact
     3781 gggattgtat tttcccgcct ctatcaaacc aatagtctgg cgtgtcaccc cgacagcctc
     3841 tgccagttga ccttgtgtta aatcacgctc tacccgagct aattttaatt ttaaattttt
     3901 agccaccttc gtcctcctta tagttttaat actcatctac tcttaaaaaa tccaaaacca
     3961 acacaagcta tcagctattt attataaccc cattaatttg cactccagta ttatcttcag
     4021 ccgaaaatca gtattttagg ttgtttgaaa taaacttcct agtttactct tttattttta
     4081 tttagtatta gctttagtag gatcttttaa acgagttttt attgcttata tttcttgttt
     4141 agtttagttg gtttcatatc actattatat aatgcttttg gaaatgagcc tagtataagt
     4201 caaaaacgtg ttgaatagtg aaagcaggag cataccaaga tgactgattt tttactgtat
     4261 tttaggtatg caaaataata ctaaatgaaa ctaaaatgaa tacttcttgc ttttcttgag
     4321 agtgaaaaag tacaataaaa tgattgtagt gattagcaca agatactgac taatagacat
     4381 cgtatttgaa gccacaaata caggtaaaga ataaaaagga agaagttcaa ttgctttgat
     4441 aaacaaatca ttactagtat tcatcatcat cgtatttaat atattaggga taaagagtag
     4501 taaagctact aaaataccaa ataccaagcc acttttcaat ttgtataaat agaaaatgta
     4561 agataataga aaataaaaca ggactaacaa aattgtattg actacaatag ctactataaa
     4621 atctaatcct aaatcacctg gaatacccaa tttacttact atgactagta acacaatgcc
     4681 attcaaagca taaataatgg aaactgagat ggcatataga aaattagaca agacgtaaat
     4741 catccgattc ttctgttgat tataaaacaa acttaccgta ttgtgggaaa aatctctact
     4801 aatgactcga ttagcatgta taacagccat taacataccg agcatagcat aaaagtttga
     4861 aatatgctta ataccaagcg atgtcccttg agtctttaga ataaagaatg taagcaatat
     4921 cggcggtata aacgagataa gcaaactgag atatattacg cctgatgaat ataaatttct
     4981 tatattttcc ttaagaaaat taaatttatt taatttcatg attataacct tcctttattt
     5041 tgaacgattt agataaatat ctttaagcgt ctctttttta gtttcaaaat caactacttt
     5101 aatagagttt tcgttaaaaa atttaaaaag ctcactactt tgaatattcc cagacatagt
     5161 aatgctcagt ccctcttcct gaacaatatc cccaaattct tgtttggtaa tgaaaatgtc
     5221 tctatctgta gctgatgaaa aagctatctc aaacaagaaa ttatgactat cttttcctac
     5281 tttttgaaat gtcaaaagcc cgttctccaa gaaaagaacc ctctcacaaa tttcttcaat
     5341 atcttctaat ttatgactcg atattaaaat tcccacattt tcatgtaaag ctaaattttt
     5401 tagaaccgat aaaactattt gtgatgattc aatatctaaa ccattagtcg gttcatctaa
     5461 aatcaatata tcaggttccg taacgagagt tagaagcaaa gctaattttt gttttgtacc
     5521 caaagaatag gtctttactt ttttattaat agactgagtc aaatctaact cttggatcaa
     5581 acttctaaat atttcttgat tgtagtcaac accatataaa tttgacaaat attttaaatt
     5641 ctctaaacct gttttagata aaaataattt tggttcttcg attaaatacc caacattatc
     5701 actgcttata atattacctg aagtcggttg attattctga acaagaattt tcatcaacgt
     5761 actcttacca actccatttc tccctgctag accaacaatt tcacccttgt taagtgcaaa
     5821 atttatatct ttgagaatga ttgaatgacc ataatgctta cttacattta taacttacat
     5881 tttttattcc tccattaata ttcatggttt gctctttgat tcttattgag tttaaaaagt
     5941 tg
//
LOCUS       DAVKWZ010000085         5850 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000085 DAVKWZ010000000
VERSION     DAVKWZ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5850
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.085"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(128..1138)
                     /gene="ccpA"
                     /locus_tag="VW854_001949"
     CDS             complement(128..1138)
                     /gene="ccpA"
                     /locus_tag="VW854_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEV0263129.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGIAYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(1563..2162)
                     /locus_tag="VW854_001950"
     CDS             complement(1563..2162)
                     /locus_tag="VW854_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0263130.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSERLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(2164..3261)
                     /locus_tag="VW854_001951"
     CDS             complement(2164..3261)
                     /locus_tag="VW854_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV0263131.1"
                     /translation="MLERLKSIHYMFWTSLIFMLFPILPVVIGELPAWHLLVDILFVV
                     TYLGVLITKNQRLSWLFWGLMLVYVAGNTAFVAGNYIWFFFFLSNLLIYHFGVRSLKS
                     LHVWTFLLAQVLVVGRLLIFQRIEVEFLVYMLVILTFVDLMTLGSVRIRLVEDLKEAQ
                     VKQNTQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQMQAYPQVEKELR
                     EIQQISKESMREVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     ESMASMILLELVTNIIKHAKASKAYLKLERTEKELILTVSDDGCGFAFLKGDELHTVR
                     DRVFPFSGEVSVISQKHPTEVQVRLPYKERN"
     gene            complement(3261..3998)
                     /locus_tag="VW854_001952"
     CDS             complement(3261..3998)
                     /locus_tag="VW854_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0263132.1"
                     /translation="MKRWIALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GEPANFMRDYLISMTAFSMMSTTILSFPAVLHTDKMNNWQKTLHHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFTVGHFVRGVDMPLVKWVGAAILLIVGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAVGKCLPTYQLMELLKTFLNEGGINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            complement(4000..4884)
                     /locus_tag="VW854_001953"
     CDS             complement(4000..4884)
                     /locus_tag="VW854_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0263133.1"
                     /translation="MTVVKVEKLSKKIKDKEILRNISFEINDGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLPVTSSKLDYTRAYLPQENVIVQKLKVKELIAFFQRIY
                     PNPLSNQEIDQLLQFVKQQKEQLAEKLSGGQKRLFSFVLTLIGRPKIVFLDEPTASMD
                     TSTRQRFWEIVQELKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     EIEKHFILPIAYKEVVEQSNLVENWTLKQDSLQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLNTIFEETQKGDN"
     gene            complement(4871..5065)
                     /locus_tag="VW854_001954"
     CDS             complement(4871..5065)
                     /locus_tag="VW854_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263134.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTDWFDQALLRQFIHGALGFGFM
                     IFVVFPMGMEWLKGESHDCG"
     gene            complement(5062..5268)
                     /locus_tag="VW854_001955"
     CDS             complement(5062..5268)
                     /locus_tag="VW854_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263135.1"
                     /translation="MGEEEMRNKMIIAMSLVVAGVMTYLMFSGLDEDFYHFPWKVFAG
                     FGIMSWLVREGLKLVRDVKKEFEE"
BASE COUNT         1865 a         1292 c          959 g         1734 t
ORIGIN      
        1 caacctcaaa acagtgtgtt gcgcagcctt taaccagccg cctagtccgc tctatggtat
       61 tcattaagtc aacatctctt gtttaagagc acaaaatcag gaaatcttct cgattccctg
      121 attttttcta tttacgtttt cgtgttgagc tacgttctgt caaaccatga ggtaagagaa
      181 cttcacgttc ttccaactct tccttatgca taatcttggt caacatacgc atactaatgg
      241 caccaaggtc ataaagaggt tgggcaatcg ttgtcaagtt tggacgggta aagcgtgaga
      301 tttgtgaatc atcactagta ataatttcaa aatcttctgg cacagaaaca cccttatcag
      361 ccaaaccgtt caagactcct gctgccaact catcacctgt cacaactgct gcagttgcat
      421 ttgatgaaat caaacgctct gctaaggcgt aaccatcatc atagctatat ttagattcaa
      481 ataccaaacc ctcactataa gcgattcctg cttttttcaa ggtttccttg tagccaacta
      541 aacgaacctt accattgatg tcatccacta gcggaccgct aacgaaagca atacgctcat
      601 tttctttagc aaggtaactc actgcatcaa ttgttgcttg cttatagtca atattgacac
      661 ttggcaactg gtgctcaaca tcgacagttc ctgcgagaac aatcggagta cgtgaacgcg
      721 aaaattctga gcgaatttta tctgtcaagt gataacccat atagataatg ccatctacct
      781 gctttgaaaa gagggtattg acaacagaaa cttctttctc gttatcttca tcgctattag
      841 ctaggacaat attgtacttg tacatttctg caatatcatc aatcccctta gccaaactcg
      901 aaaaataacc attggtaata tttggaatca cgacaccgac agtggttgtc tttttacttg
      961 caagaccacg cgcaactgca tttggacgat aatccaaacg atcaattacc tctagcactt
     1021 ttttacgggt attctctttt acatttttat tgccattgac cacacggctg accgtcgcca
     1081 tggaaacacc tgcttcacga gcgacatcat aaatggttac tgtatcatct gcattcattc
     1141 cttttcctgt cctttctatc tcacacattc ttttacaagt agaggtactg attgaagctc
     1201 tatatctact tacaaaagtg aagatgtgaa aatttcgttt tcatatttct acttattcca
     1261 ttctatcact aattgtaaac actttcaagt gttttttgaa gattgattga aaaaatttca
     1321 tagaaaacct aggtttagct ccttgctacc accttagact aaacacaaag gaggaaacta
     1381 agccctccta aagttatagt aaaatgaaat aagaacagga taaatcgatc aggacagtca
     1441 aatcgatttc taacaatgtt ttagaagtag aggtgtacta ttctagtttc aatctactat
     1501 aggtattgtt ccattcacta ccgtcaattt tagcacatag ccttcataaa aatattatat
     1561 catcataacc aaccagattc ttttgcgata ttagctgcct ctgttcgatt accagcatcc
     1621 agttttgaaa gaatattggt gacatagttt cgaatagttc cgtttgagag gtaaagctga
     1681 tctgcgattt cttggttgga taagccccgg gcgattccct ttaagacagc aatctcttgt
     1741 tctgtcaacg gattgggacg ggtcatcacc acttccatca attcaggcga atactccttg
     1801 cgtccttcga ggacggtgtg caaggtttgc atgaggtctg caatgcttcg ttcctttaat
     1861 acataagcat ccactccagc cttgaccgca cgttcaaaat acccagcacg cttgaaggtc
     1921 gtcaccacaa ccacctttgt ttcaagcctt tctgatcgta tccactccaa gacttcaaga
     1981 cctgtcttaa caggcatttc tacgtcaagg atggcgatat ctacagactc cttttctaat
     2041 agttggattg cttcttgccc attcttggct tgaaggacag actccacatc tggttgaagc
     2101 gtgagcaatt gacacatggc atctcgcaac atactttgat cttctgcgac taatactttc
     2161 atcttagttt ctctccttat aaggtagtcg aacttgcact tccgttggat gtttctgact
     2221 gattacactt acttctcctg aaaatggaaa aacacgatct cggactgtat ggagctcatc
     2281 tccttttaga aaagcaaagc cgcagccatc atcacttact gttaaaatga gttccttctc
     2341 tgtccgttct aattttaagt aagctttaga cgctttggca tgtttgatga tattggtcac
     2401 caactcaagc aaaatcatag aagccattga ctccaattct tgagtaaggc tagcagtatc
     2461 tagttggtta tccgtttcca cctcaattcc agcaatttct aacatctttt tcacagtctc
     2521 tagttcggat gtcaaagttc tagacttaag attttccaca atggttcgaa cttcacgcat
     2581 tgattctttg ctaatttgct gtatttctct taattccttt tccacttgtg gataagcctg
     2641 catttgaaat agctgcaagg ctagatctgt cttgacacta agcatagcaa aggtatgtcc
     2701 cagactatca tgcaaatcct gaccgatacg attgcgctca ttttctgcta gcaatagatt
     2761 tatctgggta ttttgcttga cctgagcttc tttcaaatcc tcgacaaggc gaattcgaac
     2821 cgagcccaaa gtcatcaaat cgacaaaagt aagaattaca agcatataga ctagaaactc
     2881 aacttcgatt ctctgaaaaa tcaaaagtcg ccccacaaca aggacttgag caaggagaaa
     2941 tgtccataca tgtaaagact ttaaactacg tacgccaaaa tgataaatta agagattgga
     3001 aaggaaaaag aaaaaccaga tataattccc agcaacaaag gcggtattcc cagctacata
     3061 gaccagcatg agcccccaga aaagccaaga taggcgctgg ttcttagtta ttaaaacacc
     3121 caaatacgtc actacaaata gaatatcaac caataaatgc caagcaggaa gctccccaat
     3181 cactacaggt aggatgggaa aaagcataaa aatcaaactg gtccaaaaca tatagtgtat
     3241 gcttttcagt ctttcaagca ttaagcattc tccttatgac cttgaaggta aatagtcaaa
     3301 ccaaataaaa ctgctgaaaa aacaagtaaa taaactgtgg ctgatagatt gatgccaccc
     3361 tcgtttaaga aggtcttgag caactccatc aattgatagg tcgggagaca cttaccaact
     3421 gcttgcatcc agtctggaaa taaagaaatg ggcatccaga gtccgcctaa aacagccaat
     3481 cctagataaa gaagattgcc cacaacagac atcagctgac tagttggtaa gagagtcaag
     3541 gtcaagccaa gtgctacaaa agcaatactc cccacaatca gcaaaatcgc agctccaacc
     3601 cattttacta gaggcatgtc tacacctctt acaaaatgtc caactgtaaa aacaaccaga
     3661 attgagacca aataatcaac cagcatactt gttatctttg atagataata ttctaccata
     3721 tttacagggc tatgatgcaa tgttttctgc cagttgttca tcttatcggt atgtaaaaca
     3781 gctggaaatg agaggatagt tgtcgacatc atggaaaagg cagtcatgga gatgaggtaa
     3841 tcacgcataa aattcgcggg ttcacctggt gtgtcctgat agataccaga aaaaaataaa
     3901 tagaaagccg tcggcatccc taccgacaat agataataga ttaattgtcg tttggtcaat
     3961 aaaaattcta tcttactaag tgctatccat cgtttcatct tagttatctc ccttctgagt
     4021 ctcttcaaag attgtattca acaaactacg attattaact tcgatttctt gcatcctaca
     4081 tcctgcttgg gctaatagtt cccagaaagc atctgcttct cgagtgacta cttgtaaaga
     4141 atcttgcttt agggtccagt tttcaaccaa atttgactgc tctacgactt ccttgtaagc
     4201 tataggaagg ataaagtgct tttctatttc ctcgctacgc atagctagag gcgtcgtatc
     4261 tcgaatcaac tctcccttat ttaagagcaa aatgcggtct gcagtgtgtt ccacttcctc
     4321 aatataatgg gacgaataga gaatggtgac tccttgcgct tttaactcct ggacaatttc
     4381 ccaaaaacgt tgacgagttg aggtatccat ggacgcagta ggctcatcta aaaaaacaat
     4441 ctttggtcgc ccaattaagg tcaagacgaa agaaaagaga cgcttttgac cgcctgataa
     4501 tttttctgcc aactgttctt tttgttgctt gacaaactgc aatagttgat caatttcctg
     4561 attgctcaaa ggatttggat agatacgttg aaagaaagca atcaactctt taacttttaa
     4621 tttctgaacg attacatttt cttgagggag gtaagcccta gtatagtcta acttagaact
     4681 cgtcactggt aaaccttgaa tagatacttg accacttgtg accagtttat ctccaagcaa
     4741 acaatctaag agtgttgtct ttccggcacc atttggacca attaaggcaa cacattcacc
     4801 atcgttgatt tcaaaggaga tgttccgcaa aatttccttg tcttttattt tcttactcaa
     4861 tttttcaact ttaaccacag tcatgagact ctcctttcaa ccactccatt cccataggga
     4921 aaacgacgaa aatcataaat ccaaacccca aagcaccatg aatgaattgg cgaagcaagg
     4981 cttgatcaaa ccaatcagta aacatttcca ctaaccatac caagagtgac aggccgataa
     5041 agaaatagat gatccctctc ttcattcttc aaactccttt ttcacatctc tgactaattt
     5101 caaaccttct ctaacaagcc aagacatgat tccaaagcca gcaaagacct tccaaggaaa
     5161 atggtagaaa tcctcatcca atcccgaaaa catgagatag gtcataactc ctgctactac
     5221 taaactcatt gcaataatca ttttatttct catctcttct tcccccattt catactacaa
     5281 ttatagtctt ttaaaatcag aggaggtaga agcttctgtc actagaaaat atgacaaatg
     5341 tcataaaaaa ttctgttcaa aacaagcaag atacactata caataaaaca caatcagaaa
     5401 aatctaaggc aacttcctca aaagagatat caaactcaat tcacaccaca atgtaaacta
     5461 atacttattt aaaatcaaaa agagtagaaa tttttatcag acaaatacat atatagtgta
     5521 ttgaatctat aacagtaggc cttaaatact aaaatatttc tataaattaa tttaactttc
     5581 ctgatagagc tgttcatatc ttatttcaat tctctatatt atacgttgaa caaaaccctt
     5641 ctattgcttt cttaaaaatt tataagagtt ataaaatctg ttaaatttca atgtgtatac
     5701 ctaaactacg gtatttattg aaaagactgg agacaaaaag tatacgctgc caaaatgaat
     5761 tactgaaaat caaaaaagag agaaccaaac tgattccctc ttaatgtata taatatctag
     5821 ttttaaaaat acacactcac atatctctgt
//
LOCUS       DAVKWZ010000086         5848 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000086 DAVKWZ010000000
VERSION     DAVKWZ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5848
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.086"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            1..993
                     /locus_tag="VW854_001956"
                     /pseudo
     CDS             1..993
                     /locus_tag="VW854_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1109..1912)
                     /locus_tag="VW854_001957"
     CDS             complement(1109..1912)
                     /locus_tag="VW854_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEV0263136.1"
                     /translation="MQPTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNTPFQQHSKY
                     FRDALVLDNAKILQRRSEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
     gene            complement(2193..2948)
                     /locus_tag="VW854_001958"
     CDS             complement(2193..2948)
                     /locus_tag="VW854_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HEV0263137.1"
                     /translation="MSKHPHYELLNLIGYGLAKFDKLFIKEFQCSSKSEFYRYVVSLG
                     IAETTGVVKNRMDLFDPYFDNNRKGWWQKAEVYRFRKDLIDMMFGNEDVHSYAEIVKM
                     LLASEGKKTGITIVEKPIVRTKFKRLQETGMEAENYFILHFDKEEKFQGGQLTDARLY
                     GDGYDFQVDVQEYSYLAEVKGIRKSKGRVRLTAKEFEKVKEFQSDFILSLVTNLDDIP
                     KLVLIDNPLKHFEFKKNIIKNEIIEYRSVEDLY"
     gene            complement(3018..5669)
                     /locus_tag="VW854_001959"
     CDS             complement(3018..5669)
                     /locus_tag="VW854_001959"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEV0263138.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAIAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNIMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNGGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDDKVITRSVLLYTLDKI
                     LRLLHSIMPFVTEEIFGQYAEGSIVTAAYPTVNPAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIFLPLVDLLNVEEELARLEKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     EKDKQADYQAKYDVTVARIDEMKKLVK"
     gene            complement(5681..>5848)
                     /locus_tag="VW854_001960"
     CDS             complement(5681..>5848)
                     /locus_tag="VW854_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HEV0263139.1"
                     /translation="KLKHSRYYISKISKYDLKYNPGNYTMSPLAAFAEIEEIYIELSK
                     IGLVKIVNKSN"
BASE COUNT         1778 a         1222 c         1125 g         1723 t
ORIGIN      
        1 ccaagtcgta cgatacttct taactggcta gcacaataca ggaaaaacgg gtatactatt
       61 gttgagaaaa caagagagag agtacctgag atcggagaat gccatcctaa aaaagttaag
      121 agaactccga ttgaaggagg aaaaagagaa agaagaaaga cagaaattgt tcaagaatta
      181 atgactgagt tttcgttaga tcttcttcta aaagccatta aactagctcg ttcgacctac
      241 tactatcact tgaaacagct agataaacca gataaggacc aagagcttaa agctgaaatt
      301 caatccatct ttatcgaaca caagggaaat tatgcttatc gtcgaattta tttagaacta
      361 agaaatcgtg cttatctggt aaatcataaa agagttcaag acttgatgaa agtactcaat
      421 ttacaagcta aaatgtgaca gaaacgaaaa taaaggagac gttggtaaga aggcagagaa
      481 tctcattcaa ggccaatttg aaggctctaa aacaatggaa aagtgctaca cagatgtgac
      541 tgaatttgcc attccagcaa gtactcaaaa gctttactta tcaccagttt tagatggctt
      601 taacagcgaa attatcgcct ataatctttc aacttcaccc aacttagaac aagtgaagag
      661 tatgttagaa caggccttta cagagaaata ctatgagaat acgattctcc atagtgacca
      721 aggctggcaa tatcaacacg attcttatca tcggttctta gagagtaagg gaattcaagc
      781 atccatgtca cgcaagggaa acagccaaga caacggtatg atggagtcct tctttgggat
      841 tctgaaattt atggctatga gaaaacattt aaatcactta atcaattgga acaagctatt
      901 gtagactata ttgattacta caacaataaa cgaattaagg taaaactaaa aggacttagc
      961 cttgtgcaat acagaactaa atccttcgga taaattattt gtctaacttt ttggggtcag
     1021 tacacctaaa actttgatga tatacgtttt cttgtgagaa tatttacttc atttttgcct
     1081 aaaattcaat gtttactcag tatttggatt atgaaaaatc gaggtctaaa tctagataca
     1141 ttttttctga agacaaatca ttttgaccac cgagcaagag attttcaaaa aaagctgtta
     1201 aaaactcaga acgtcgctgt aaaatctttg cattatctaa taccaaggca tcacgaaaat
     1261 atttggaatg ttgctgaaat ggtgtattat caatatcaaa accaaactca cgaagatact
     1321 gaatcaaaaa gaccgttact gtccgagtgt ttccttcgcg aaatggatga atctgccaga
     1381 ttcctgaaat aaaatgctgg atttgtttaa ccacatccgc ctgagttagt gtcgcatatg
     1441 caacttgttt ttcctgatta aaatcataat ctaaggtcat ttgaatcatg gagtaatcag
     1501 agtacacaac actttcacca ttcaaaacag gttcattctt tgtgatattg gtctgacgaa
     1561 attgccccac cggaatagag ggttcaaata tatcttgaaa caactcctta tgaatagcaa
     1621 gtaaggtcgc aggactaaag ctaaagcctc ttcgagacaa tagttctaca atacgtagag
     1681 aaaccaagtc tgcctcttcc gtacttgcat caatagtatg gtgataagcc gttgcatcct
     1741 cataaacctg ctcataagtc agttctcccc gggactgttt ctcagccaaa gattccatat
     1801 acgctgatgg cactagattg tcaactttct gcagaccaaa acctatccgc cataaatcac
     1861 gcttcgcttc ataagacaag tttggattgt caatgttgta agttggttgc atagaaatat
     1921 cctttcaatt gtttttcttc attaccagta aatcacgaaa ttggttggaa actggcttaa
     1981 actataaaaa gtcctcacaa taggttctat gatatatata tatatatact tcatgtaaat
     2041 aataaaagag ttataatcag cactactgat aaattcaata ccgatttcgc catcttaaaa
     2101 cttagtcaaa cgaataccgt tgatttccag ttcattaagt cgttgacctg tcaagacttc
     2161 tcgttccttc aactggaaaa gttaaggggg aactaataca aatcctctac gcttctatat
     2221 tcaatgattt catttttgat aatattcttt ttaaactcaa aatgttttaa aggattatcg
     2281 attaacacta actttggaat atcatctaga tttgtgacca aggataaaat aaaatccgat
     2341 tgaaactcct tgactttttc aaattctttt gcagttaaac gcacacgccc cttagatttt
     2401 cggatacctt ttacttcggc aaggtaagaa tactcttgga catctacctg aaagtcatat
     2461 ccatcaccat aaagacgggc atcggttaac tgtccacctt ggaatttttc ttctttatca
     2521 aaatgaagga taaaataatt ctcagcctcc attcccgttt cctgtagacg tttgaattta
     2581 gttcgaacta ttggtttttc aacgatggtt atgcctgttt tcttcccttc actggcaagt
     2641 aacattttaa ctatttcagc ataactatga acatcttcat ttccaaacat catatcaatt
     2701 aaatctttac gaaaacgata aacttcagct ttctgccacc aaccttttcg attattgtca
     2761 aaataaggat caaataaatc cattctattt tttacaactc cagttgtttc agcaattccc
     2821 agagaaacca catagcgata aaactccgac ttgctagagc attgaaattc ttttataaaa
     2881 agcttgtcaa acttggcaag accgtagcca attaaattta acaattcata atgagggtgt
     2941 ttggacatca ctttctccat caatagatat tgatttcatt ataccaaaaa acacggctca
     3001 tcaccgtgtt tctgtgttta tttcaccaac ttcttcatct catcaatacg tgctacggtc
     3061 acatcatact tggcttggta gtcggcttgt ttgtcttttt ctttttggac aacttctggt
     3121 ttagcgttgg cgacgaagcg ttcgttgctg agttttttgc caaccatgtc gagttctttc
     3181 tgccatttgg caagttcttt ttccagacgt gccaattctt cttcgacatt caagaggtct
     3241 accagtggca agaagatttc ggctcctgta ataacgcttg acatagccag ttcaggtgca
     3301 gggatggttg atgcgatttc caagtgttct ggatttgtaa agcgtttgat gtagttgaca
     3361 ttgctgttaa agaaggcttc caagtcacta tcgctagtct taacaaggat ggtgataggt
     3421 ttgcttggtg ctacatttac ttccgcacgc gcattacgaa cggcacggat caagtctttg
     3481 agactttcca caccagtgtg ggcagcaagg tcctcaaagg ctgggttgac agttgggtat
     3541 gctgctgtca cgatagagcc ttcagcgtac tgaccaaaga tttcttctgt cacgaacggc
     3601 attattgagt gaaggagacg aaggatttta tccagcgtat agaggagaac agatcgagta
     3661 attaccttat cgtcttcatt gtcgctgtaa agaacttcct tggtcaactc aacataccag
     3721 ttggcaaatt cttcccaaat gaagttgtaa aggatatgac cagccacacc aaactcgaac
     3781 ttatcaaagt tttcagtaac ttttgcaatg gtttcgttga gattgtggag aatccagcgg
     3841 tccgtcacat taccagcctc acctgttgca acttttgtga cattgtcatg cgccacatcc
     3901 agcgtcaaac ctccattgtt catgaggata tagcgagaaa tgttccaaat cttgttaata
     3961 aagttccatg aagcatccat tttctcataa gagaaacgca cgtcttgtcc tggcgcagaa
     4021 ccgtttgaaa ggaaccatcg aagggcatcg gcaccgtatt tctcgataac atccattggg
     4081 tcaatcccgt taccgagaga cttagacatc ttgcgtcctt gctcgtcacg aatgagaccg
     4141 tggataagga cgttttggaa tggttgacgg cctgtgaatt ccaatgactg gaagatcata
     4201 cgagacaccc agaagaagat gatgtcgtaa cctgttacca aggttgaagt tgggaaataa
     4261 cgtttaaagt cttctgagtc gacttcaggc cagcccatgg ttgaaaatgg ccagagggca
     4321 gaactgaacc aagtatccaa gacgtcttcg tcctgagtcc atccgtcacc ttctggagct
     4381 tcttcgccga catacatttc accatcagca ttgtaccaag cagggatttg gtgaccccac
     4441 cagagctgac gagagataac ccagtcgtgg acattttcca tccattgaag gaaggtatcg
     4501 ttgaaacgag gtgggtagaa ttcgaccttg tcctctgtgt cttggttggc aatggcgttc
     4561 ttagccaatt ggtccatctt gacgaaccat tgagtagaca agcgtggctc aactacgaca
     4621 cctgtacgtt ctgagtgacc aacactgtgg acacgttttt cgattttgac aagggcaccg
     4681 atttcttcca acttagcaac gactgcctta cgagcttcaa aacgatccat gcctgaaaat
     4741 tcaaaggcaa gctcgttcat agttccgtcg tcgttcatga tgttgacttg tggcaagtta
     4801 tgacgttggc caaccaagaa gtcatttgga tcgtgggcag gtgtgatttt cacgacacca
     4861 gtaccaaact caggatctgc gtgctcatct ccaactattg ggatgagttt attagcgatt
     4921 ggaaggatga cgtttttacc aatcaagtcc ttgtagcgcg ggtcttctgg attaaccgca
     4981 atcgcaacgt ccccaaacat agtctcagga cgagttgtag caacttcaag ggcgcgtgaa
     5041 ccatcttcca gcatgtaatt catgtggtag aaggcacctt ccacatcctt gtgaatcacc
     5101 tcaatatcag aaagggctgt gcgagctgct gggtcccagt tgatgataaa ctcaccacgg
     5161 tagatccagc ctttcttgta aaggtccaca aagaccttac gaacagcttt tgacaaacct
     5221 tcgtcaagag tgaaacgctc acgagaatag tctacagaga gccccatctt gccccattgt
     5281 tccttgatgg tagtggcata ttcgtctttc cattcccaga ctttcgtcaa gaaagactca
     5341 cgaccaaggt catagcggga aatgccctca ccacgcaagc gctcctcaac cttagcctga
     5401 gtggcaatcc ctgcgtggtc catcccagga agccaaaggg tatcaaaacc ttgcatgcgt
     5461 ttttgacgga tgataatatc ttgcaaagtt gtatcccaag cgtgaccaag gtgaagttta
     5521 cctgtaacgt ttggtggtgg aataacgatt gaataaggct tagccttttg atcgcctgaa
     5581 ggcttgaaaa catcagcatc aagccatttt tggtaacgac cagcctcaac ctcggctgga
     5641 ttgtatttag atgaaagttc tttagacatg tgtgttctcc ttaattactt ttattgacaa
     5701 ttttaaccaa accaattttt gaaagttcaa tataaatctc ttcaatttca gcaaatgctg
     5761 caagtggtga catagtataa tttccaggat tatatttaag atcatacttg cttatctttg
     5821 aaatataata cctagagtgt ttcaattt
//
LOCUS       DAVKWZ010000087         5676 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000087 DAVKWZ010000000
VERSION     DAVKWZ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5676
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.087"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(<1..237)
                     /locus_tag="VW854_001961"
     CDS             complement(<1..237)
                     /locus_tag="VW854_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263140.1"
                     /translation="MKILNIELANVEQTDLGFEHWVDVTYQVPILKNEYTVKLLLLME
                     CRIEDQEVIEYLVSTWKYRDLVLHSVRMYEIEKSE"
     gene            complement(508..1464)
                     /gene="lytA"
                     /locus_tag="VW854_001962"
     CDS             complement(508..1464)
                     /gene="lytA"
                     /locus_tag="VW854_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEV0263141.1"
                     /translation="MDIDRNRLRTGLPQVGVQPYRQVHAHSTGNRNSTAQNEADYHYR
                     KDPELGFFSHVVGNGRVMQVGPVDNGSWDVGGGWNTESYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTDDLEGIKTHEYCTNNQPNNHSDHVDPYPYLASWGIS
                     REQFKQDIENGLSAATGWQKNGTGYWYVHSDGSYPKDKFEKINGTWYYFDGSGYMLSD
                     RWKKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYIKPDGTLADKPDFTVEPDGLITVK"
     gene            complement(1464..1799)
                     /locus_tag="VW854_001963"
     CDS             complement(1464..1799)
                     /locus_tag="VW854_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEV0263142.1"
                     /translation="MQQITEIITNGAISILVILAGIAVKAVKEYLVKKGGEKTIKIVE
                     ILAKNAVNAVEQVAAETGYKGDEKLAQARAKVRAELTKYNISMTDKDLDTFVESAVKQ
                     MNDAWKGQE"
     gene            complement(1803..2102)
                     /locus_tag="VW854_001964"
     CDS             complement(1803..2102)
                     /locus_tag="VW854_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263143.1"
                     /translation="MPIEEAEKIAQSQVAWAILFILLFFIIIRYLIKTSDKREKKIMD
                     LHEQSKADSNRREERLMTHLEKTTTELTTITHTVGDIQKEMVRMNDRMEEIEKGE"
     gene            complement(2111..2461)
                     /locus_tag="VW854_001965"
     CDS             complement(2111..2461)
                     /locus_tag="VW854_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263144.1"
                     /translation="MAETIQNTDNLLDLTKITEPFDLASALRYMKENGEFIRCKNVSD
                     DFYMYRDVQKRPVIVNGRRQLKDVETVWAFNQWGGTIATINVAVLLNHEFYIMKFDAE
                     GNPDWTVPTVEPKE"
     gene            complement(2464..2667)
                     /locus_tag="VW854_001966"
     CDS             complement(2464..2667)
                     /locus_tag="VW854_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263145.1"
                     /translation="MNTEQLNQALRMTISEISTTSTNSMITSNILSIQLDEQREENQR
                     LQARVDELEALLDEQTKPADKGE"
     gene            complement(2648..4029)
                     /locus_tag="VW854_001967"
                     /pseudo
     CDS             complement(2648..4029)
                     /locus_tag="VW854_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838376.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
BASE COUNT         1447 a         1325 c         1014 g         1890 t
ORIGIN      
        1 ctcactcttt tctatctcat acatccttac cgaatgcaac acgagatcac gatacttcca
       61 agtgctgacc agatactcaa taacctcttg gtcctctatc ctgcattcca taagtaataa
      121 tagcttgacc gtgtattcat tcttcaaaat cggaacctga taagtcacat ctacccaatg
      181 ctcaaaacct aagtctgtct gctctacatt tgctagttca atatttaaaa tcttcatttt
      241 tattcctcct acttatctat tcgtaaaaga agataaaaag ttatgaaaaa atcattattt
      301 ttttattttt aacaaaacac cgtttttgac aataatcaaa aactaaaaag gagctattat
      361 taacaaaatg gcatttttga caataatgct cttccattcc tcctccctat tcttcatgta
      421 aacgcttttt tgaagaatca gagcaaacaa aaaaaccgca agcctgagcc tgcggtgaaa
      481 gaacaattta gaaagtttcc tttctattta tttaactgta atcaagccat ctggctctac
      541 tgtgaagtct ggcttgtctg ccagtgttcc gtctggtttg atgtagtacc agcctgttcc
      601 gtctgctgac tggataaagg catttgatac catggcgcct tctttagcgt ctaagtagta
      661 ccaagtgtcc ttgtacttga cccagcctgt cttcatggca ccttcttcgt tgaaatagta
      721 ccacttatca gcgattttct tccagcctgt agccatttcg cctgagttgt cgaaccagta
      781 ccagttgccg tctgtgtgct tcttccagcg gtctgaaagc atatagcctg agccatcgaa
      841 ataataccag gtaccgttga ttttctcaaa cttatctttt ggataagagc cgtctgagtg
      901 tacgtaccag tagccagtgc catttttctg ccagcctgtt gcagcgctca agccgttttc
      961 gatgtcttgc ttaaactgct cacggctaat gccccaactt gcaagatatg gatatggatc
     1021 cacatggtct gagtggttgt ttggttggtt attggtacag tattcatgcg tcttgatacc
     1081 ttccaagtcg tctgtatcaa gagtcttcgg caaacctgct tcatctgcta gattgcgtaa
     1141 gagttcgata taaaggcggt agtccgtcat gaactcttct ttagttgaat ggctttcaat
     1201 cagttcaacc gctgcgtaac tctcagtatt ccaaccgccc ccaacatccc aacttccgtt
     1261 gtccacagga cctacttgca tgacacggcc atttccgaca acatgtgaaa agaaccctag
     1321 ttcagggtcc tttctatagt ggtaatcagc ctcattttga gctgttgagt tacggttgcc
     1381 tgttgagtgg gcgtgtactt gtcgataagg ctgcacccca acctgtggca agcctgttcg
     1441 tagtctgttt ctatcgatat ccattactct tgtcctttcc aagcgtcgtt catctgcttc
     1501 acggctgact ccacaaaggt gtctaagtcc ttgtccgtca tactgatatt gtattttgta
     1561 agctcagcac ggactttagc acgagcctgt gccagttttt catctccctt gtagccagtt
     1621 tcagcagcta cctgctccac ggcattaact gcgttcttag ccaagatttc aacaatcttg
     1681 atagtctttt ctccaccttt tttgacgagg tattccttga ctgccttgac tgcgatacca
     1741 gccaaaataa caaggatgct gattgctcca ttagtaatga tttcagtaat ttgttgcatt
     1801 tgttattctc ctttttcgat ttcttccatg cggtcgttca tgcgaaccat ttctttttga
     1861 atgtctccga ccgtgtgagt gattgtggtt aattctgtag tggtcttttc taggtgagtc
     1921 atcaaacgct cttctcgtct attagagtcg gcctttgatt gctcgtgcaa atccataatc
     1981 ttcttctctc gcttgtccga agtcttgata agatatcgaa tgataataaa aaaaagcaag
     2041 ataaacaaaa tcgcccaagc tacctgactt tgagcgattt tttcagcttc ttcaattggc
     2101 atacaacctc ctattcttta ggttctaccg ttggaaccgt ccagtcagga ttgccctctg
     2161 catcaaattt catgatatag aattcatgat tcaacagaac ggcgacgttg attgttgcga
     2221 ttgtaccacc ccactggttg aacgcccaaa cggtttcaac atccttgagt tggcgacggc
     2281 catttacgat cacaggacgt ttttgaacat cacgatacat atagaagtca tcgcttacat
     2341 tcttgcaacg aatgaactct ccattttctt tcatgtagcg caaagcactc gcaagatcaa
     2401 atggttctgt gatttttgta aggtctagta agttatctgt gttttgaatt gtttctgcca
     2461 tgtctattct cctttgtctg ctggtttagt ttgttcatca agcagagctt ccagctcatc
     2521 cactcgtgct tgaagtcttt gattttcttc cctttgctca tccaactgaa tactcaagat
     2581 attacttgta atcatcgaat ttgttgaagt tgttgatatt tcactaattg tcattcgtaa
     2641 ggcttggtta agctgttctg tgttcatttt ctaagttctc caatctgtgt gttcgttttc
     2701 tattttcaag agcaagctcc tgaattgctt taagtgcgat attggtcaac atgttgttat
     2761 tcatgctatt gttttctcca ttttttctat tttttgattt aattcttgaa tagccttgat
     2821 taagtaagga actaaagcgg tatagtcgat atgtagatag ccatctggat tctcaggatc
     2881 tcgtgagaca attcttggaa cgatggtttc agcctcttga gctattagac caatctcctc
     2941 atgtttctta ttttcgatga aatcaaatgc aaccattctt aatctgttga ttttgtccaa
     3001 ggctttcaca gctgtatctg tgatgttctc ttttaagcgt ctatctgatt tttgttccat
     3061 ccaatacttc aagctaccgc taccgacctg attccaccaa acaaccgcat tccttccgcc
     3121 tttgggattc caaccatcac caagcacatc tttacttcca agttcgatac catttgaaaa
     3181 cacaggagaa cgagaaaaag tagtattccc atagaagttt gctctcgatg aattcgaaaa
     3241 atccacttga tcataaaaac cgacttcatt cctacagtac attttcccat cagtattgac
     3301 gttccatgct ttaggtccgg catagttcca attatttccc cagttcgccc agaaggctgt
     3361 ccggactcca tgcccggcac cattccccat accaacagag aactgattga cacctgaaat
     3421 ccagcgaccg ccaccctggt caaattgacc aagtgtgaat ccaccaattc ggccttgata
     3481 ggcttctagg aaggttgagc tagaaatgac ggactcaacc ttagtagaga agatacgttt
     3541 agatgtcagt cggtcaataa aagcatcttt tgcagttaat tttctaataa gcgcatcgtc
     3601 aactttcaac ttctcagcag ttaccgcttc agcttctaat atcgtagtcg tgaccgaacc
     3661 agcttcaaaa ttggccgttt tgagcttatc aaccatggca gacttgatga ctgctctgtc
     3721 aatcagggtc tctccagtga tgtgggtcaa tttcccaact aagcggttat gtccattggc
     3781 gccaagattg attccagaga tgatatctcc agccgagttg atgttttcaa ctacccatga
     3841 gccagctagt tgagtcattt ttgtttgcgt tgcttcaagc ttcttattcg catctgcgac
     3901 tgcatcttct ggatgtggtt gccatgttct aggtttataa cctttgtaca agtcaacttc
     3961 tgtaatatac aaatcagctg ttcctgatga tgagccattg ttatcaaaac gaatgtaagc
     4021 attatccatt tctccggaat taaaagttac tgagacatct tcgcatctag aggtagatag
     4081 tttcttgctg ctaacaactt tcttaacgat tgtgaatcca tcgctctcgc ctgctcttcg
     4141 tcccaaaata taaacatcat agcttgctag agcactgttg ttaaatcctc taaaattcag
     4201 tacatagtca gtatttcgtt caagattaaa acggtgacta tacaaaaagt tttcgttttt
     4261 agttgcatta cttaaacgca taaggtcttt ctgcccgttg tgataaaagc tatgcttaac
     4321 caatcttcct aaattttgag ttgagcccca ttcattcgta gcatttttaa aatcactatt
     4381 cttaatgagg ttagggccgc ttacactata tttcccaacc tcaacctgaa atagttgatt
     4441 ggtcagagcc atgcgagcaa ccttatccgc aattccattt tcagtattgc ccaaaatccg
     4501 ctcgtaaagt ttactggttt ccttaacacg ctggaagtca gtagtctcta cttttcgagc
     4561 tagttgattg gtcacattcg caaattgact atcagcattc gctttgtttg cagaaacctg
     4621 atcagatatt ctacccattt gtcgttcagc attatccttg tttgtagcga cctgagtctt
     4681 taaatttgaa atctgagtag tggttccttg ctcactgctt gtaagtctat ttgatagacc
     4741 actgatttga ccgcccacat cttgcttata agtagttatc tgacttgaaa tatccgtgaa
     4801 cttaccatct acagattgac gatagctagc gatttgacta gcgatgtctt tattcgcact
     4861 agttttaaca gcttcaatcc tctgattgat acccttaaca tcttcttgat aagtagcctt
     4921 accaacgaaa tcacgattga ccagctcacg gactgctgtc gcttgtctcg tgctctcctc
     4981 acgagtatag cgctgtaggg cttcctgtcg ctgaccgtct ttatttacat attcctgaat
     5041 agctgataaa tcggttcgca agccctgagc tgtccgctca aaggtagcct tagcctcagt
     5101 gatgagacca tcagcgtcct caggcgcagg actccagtcc gtcgccacac taccaatttc
     5161 aaccttgatt cctgttaccc aagctgtacc gcttgtagca ccttcaagat tgaatcgcaa
     5221 tgatgtcttc aattgatcaa aatttgtttt ttcagagtag tcataagtga atgtaatata
     5281 tttccaatct gccgaacctt tatacatacc aagcgtagca taatctggac cactctgtac
     5341 tccggtctca ctattttttc taaaaagata atgtttgaag caattaaata cattccaaaa
     5401 atttcgacct tggactacat tttcgtactt gatccaagcg ctaaaagtaa ctttttgata
     5461 caaccttgag ctgaaatctg gttcaatgtt gaacattaaa gtggagttgt tctctagcct
     5521 atagcattct ttttgacctg tgacgtggtt ttcaggtaat ttttcaatta cagctccaac
     5581 cgtcttggat tttatccata gattccgtcc tcccaccttc atttttgaaa attcttcacg
     5641 caatttccca gcttcagata caactaaagt cttatc
//
LOCUS       DAVKWZ010000088         5581 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000088 DAVKWZ010000000
VERSION     DAVKWZ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5581
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.088"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            1273..2532
                     /locus_tag="VW854_001968"
     CDS             1273..2532
                     /locus_tag="VW854_001968"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV0263146.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAKGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            2548..3099
                     /locus_tag="VW854_001969"
     CDS             2548..3099
                     /locus_tag="VW854_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0263147.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLFIITHLENKASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            3092..4369
                     /locus_tag="VW854_001970"
     CDS             3092..4369
                     /locus_tag="VW854_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV0263148.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQIHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            4385..5245
                     /locus_tag="VW854_001971"
     CDS             4385..5245
                     /locus_tag="VW854_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEV0263149.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
BASE COUNT         1683 a          906 c         1258 g         1734 t
ORIGIN      
        1 gcgtcgtagt ccagcttagc gatgatttct ttgtgtgaat ccctattgac gacatctata
       61 atttggatat ttgtgtcttt gatatcgagt agttttgtga taaaatgtaa ttgtcccaca
      121 tgaatctttc taatgagttg tttgatcgct tttcattata gatcttatgg gacttttttt
      181 ctacaataaa ataggctcca taatatctat agtggattta cccactacaa atattataga
      241 gccaaaaatc ccgtgcctca tcagacacgg gattttgtgg tacgacaggc atgtatagca
      301 aactgaatct ggaatagcac agcatatctt ctaaaatatt gttataaatt gatttgaatt
      361 tcaaaattaa attgtttgat tcttatttca atttgttata gtatatctga tgtcaaagtt
      421 ctcggcgagt caaatagcga ttcccaagcc tgactatcgt gaggtagcgg attaaaatgg
      481 tctggggata gaccgtttta agtctgacgc tggaaataag aattgtcaga agaagggata
      541 gcgaaatcgt ggctctacga acaggaacgt gataataagg cgtatatagc ggataagagg
      601 gcatcaaact ctaaagtcca aaaaggtagt cgtaacctat atgcgtaaat cacgagagta
      661 attgaattcg tactaagatt ttctattttc actgtaacct tttaacgccc ttatatcttg
      721 tatacacgag gaaagatgta cgacttatcc cgtgaggtct atcactataa agagaaaacg
      781 acagatagaa gtgatcctga gtcacggtta tctgtctgat aggacggtat gtataaaacg
      841 cttctgtgaa ctgagagaag ggggagaagt tcttgctaaa atttagttga acagccgtat
      901 tccgatactt agataagaga tctagtctta gctcctactc agttttaggg gataaaaaag
      961 gggcaatagc gattcgagaa agattatact cttcgaaaat ctcttcaaat cacgtcaata
     1021 tcgccttgtc gtatgtgtag gatactgact acgtcagttc catctacaac ctcaaaacag
     1081 tgttttgagc aacctgcggc tagtttccta gtttgatctt tgattttcat tgagtattag
     1141 taattcagtt actaactcgt caactctgat ttatccaata aaattgaaaa ggatggaaaa
     1201 aaggataaat ttatgatata ctttattttg aagaccttat tagaaatctt gaaagagtat
     1261 tgaaaactta gaatgagaaa aattgttatc aatggtggat taccactgca aggtgaaatc
     1321 actattagtg gtgctaaaaa tagtgtcgtt gccttaattc cagctattat cttggctgat
     1381 gatgtggtga ctttggattg cgttccagat atttcggatg tagccagtct tgtcgaaatc
     1441 atggaattga tgggagctac tgttaagcgt tatgacgatg tattggagat tgacccaaga
     1501 ggtgttcaaa atattccaat gccttatggt aaaattaaca gtcttcgtgc atcttactat
     1561 ttttatggga gcctcttagg ccgttttggt gaagcgacag ttggtctacc gggaggatgt
     1621 gatcttggtc ctcgtccgat tgacttacac cttaaggcgt ttgaagctat gggtgccact
     1681 gctagctacg agggagataa catgaagtta tctgctaaag atacaggact tcatggtgca
     1741 agtatttaca tggatacggt tagtgtggga gcaacgatta atacgatgat tgctgcggtt
     1801 aaagcaaatg gtcgtactat tattgaaaat gcagcccgtg aacctgagat tattgatgta
     1861 gctactctct tgaataatat gggtgcccat atccgtgggg caggaactaa tatcatcatt
     1921 attgatggtg ttgaaagatt acatgggaca cgtcatcagg tgattccaga ccgcattgaa
     1981 gctggaacat atatatcttt agctgctgca gttggtaaag gaattcgtat aaataatgtt
     2041 ctttacgaac acctggaagg gtttattgct aagttggaag aaatgggagt gagaatgact
     2101 gtatctgaag acagcatttt tgtcgaggaa cagtctaatt tgaaagcaat caatattaag
     2161 acagctcctt acccaggctt tgcaactgat ttgcaacaac cgcttacccc tcttttacta
     2221 agagcgaatg gtcgtggtac aattgtcgat acgatttacg aaaaacgtgt aaatcatgtt
     2281 tttgaactag caaagatgga tgcggatatt tcgacaacaa atggtcatat tttgtacacg
     2341 ggtggacgtg atttacgtgg ggccagtgtt aaagcgaccg acttaagagc tggggctgca
     2401 ctagtcattg ctgggcttat ggctaaaggt aaaactgaaa ttaccaatat cgagtttatc
     2461 ttacgtggtt attctgatat tatcgaaaaa ttacgtaatt taggagcgga tattagactt
     2521 gttgaggatt aaaccgtaga ggtgtttatg aatatttgga ccaaattagc aatgttttct
     2581 ttttttgaaa cggatcgctt gtatttgcgt cctttctttt ttagtgatag tcaggacttc
     2641 cgcgagatag cttcaaatcc agaaaatctt caatttattt tcccaacgca ggcaagtctg
     2701 gaagaaagtc aatatgcact ggccaattac tttatgaagt cccctttggg agtgtgggca
     2761 atttgtgacc agaaaaatca acaaatgatt ggttctatta aatttgagaa gttagatgaa
     2821 atcaaaaaag aagctgagct tggctatttt ttgagaaaag atgcttggtc gcaaggattt
     2881 atgacagagg ttgttagaaa aatttgtcag ctttcttttg aggaatttgg cttaaaacaa
     2941 ttatttatca ttacccacct tgaaaataaa gctagccaaa gagttgctct taagtctgga
     3001 tttagtttgt tccgtcagtt taagggaagt gatcgttaca caagaaaaat gcgggattat
     3061 cttgaatttc ggtatgtaaa aggagagttc aatgagtaag catcaggaaa ttctaagcta
     3121 tttggaggaa ttaccagtag gtaaaagggt cagtgttcgt agcatttcga atcatctagg
     3181 agttagtgat ggaacagcct atcgggctat taaagaagct gaaaaccgtg gaattgtgga
     3241 gacccgtcct agaagtggaa caattcgtgt taaatcccag aaagttgcta tagagagatt
     3301 aacgtttgct gaaattgcag aagtgacttc ttctgaggtt ctggctgggc aagaaggttt
     3361 agagagagaa tttagtaagt tttcaattgg tgccatgact gaacaaaata tcttgtctta
     3421 ccttcatgat ggggggctct tgattgtcgg agaccgaacc cgtattcagt tgctagcctt
     3481 ggaaaatgaa aatgcagttc tggttacagg gggatttcag attcatgatg atgtgcttaa
     3541 actggccaat caaaaaggga ttcctgttct aagaagtaag catgatacct ttaccgtcgc
     3601 gaccatgatc aataaagcct tgtcaaatgt ccaaatcaag actgatattc tgacagttga
     3661 gaaactttat cgccctagtc atgagtatgg ttttctgaga gagacagata cagttaaaga
     3721 ttatttggac ttggttcgta agaatcgtag cagccgtttc cctgttatca atcaacatca
     3781 ggtcgttgtt ggtgttgtaa ccatgagaga cgctggtgat aaatcaccaa gcacgacaat
     3841 tgataaggtt atgtctcgta gtctattttt ggttggatta tcgacaaata ttgccaatgt
     3901 gagtcaacgg atgatcgcag aagactttga aatggtacca gttgttcgaa gcaatcaaac
     3961 tttgcttggc gttgtgacgc gacgagatgt catggagaag atgagccgtt cccaagtttc
     4021 ggctctacca actttttctg agcagattgg acaaaagctc tcttatcacc atgatgaagt
     4081 agtcattaca gtggaaccct ttatgctaga aaaaaatgga gttttggcta atggtgtatt
     4141 ggcagaaatt ctgacccaca tgacccaaga tttagttgtt aatagtggtc gcaatctcat
     4201 tatcgagcag atgctgatct actttttgca ggctgttcag atagatgata tattgcgcat
     4261 tcaggcacgg attattcatc atacgagacg gtcagctata attgattacg atatttatca
     4321 tggtcaccag attgtttcaa aagcaaatgt gactgttaaa attaattaga aactaggaga
     4381 aaagatgata acattaaaat cagctcgtga aatcgaagct atggacaagg ctggtgattt
     4441 tctagcaagt attcatatag gcttacgtga tttgattaag ccaggcgtag atatgtggga
     4501 agttgaagaa tatgtccgcc gtcgttgtaa agaagaaaat ttccttccac ttcagattgg
     4561 ggttgacggt gccatgatgg actatcctta tgctacctgt tgctctctta acgatgaagt
     4621 ggctcacgct ttccctcgtc attatatctt gaaagatggt gatttgctca aagttgatat
     4681 ggttttggga ggtcccattg ctaaatctga cctgaatgtc tcaaaattaa acttcaacaa
     4741 tgttgaacaa atgaaaaaat acactcagag ctattctggt ggtttagcag actcatgttg
     4801 ggcttatgct gttggtacac cgtccgaaga agtcaaaaac ttgatggatg taaccaaaga
     4861 agctatgtac aagggtattg agcaagctgt tgttggaaat cgtatcggtg atatcggtgc
     4921 ggctattcaa gaatacgctg aaagtcgtgg ttacggtgta gtgcgtgatt tggttggtca
     4981 tggtgttggc ccaactatgc acgaagaacc aatggttcct aattatggta ttgcaggtcg
     5041 tggactccgt cttcgtgaag gaatggtctt aaccattgaa ccaatgatca atacaggcga
     5101 ttgggaaatt gatacagata tgaaaactgg ttgggcgcat aagaccattg acggtggatt
     5161 gtcatgtcag tatgaacacc aatttgtcat tacgaaagat ggacctgtta tcttgactag
     5221 ccaaggtgaa gaaggaactt attaataaaa agtgaaaaga ctactggaag tttattttga
     5281 taaaaaatcc agtagatctt ttcataataa aacgcattgt atcaagtgtt aggggctgat
     5341 atcatgcgtt tttctgcttt taagattttt tccaactctg tttgtaagcg catcataaca
     5401 aagggtctag gattcagggc tctcctccta tatactatta gtaaagtaaa actaagggag
     5461 gatattttag tgtcgcagtc tattgttcct gtagagattc cacaatattg tcgttttgat
     5521 tctaaaaaga gaaatggaat tctgtttaat gttcgtattg ccaatcttaa atttactttt
     5581 t
//
LOCUS       DAVKWZ010000089         5523 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000089 DAVKWZ010000000
VERSION     DAVKWZ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5523
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.089"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(<1..317)
                     /locus_tag="VW854_001972"
     CDS             complement(<1..317)
                     /locus_tag="VW854_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0263150.1"
                     /translation="MNRLKELRQEKKLSQKEIAETLGFSLRSFQRMENGESQIKPEKA
                     QLLADYFGVSVANLLGYENNFIESVKNLSQKDGSDEVFFKAFRAYYELKTADGRENLL
                     TLK"
     gene            536..736
                     /locus_tag="VW854_001973"
     CDS             536..736
                     /locus_tag="VW854_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV0263151.1"
                     /translation="MSKLRGYRVMLGLTQQQMADKLKISLQSYNNKELGKTPFNDKER
                     LAIKSMVAEIKPDITIDEIFYS"
     gene            921..1349
                     /locus_tag="VW854_001974"
     CDS             921..1349
                     /locus_tag="VW854_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263152.1"
                     /translation="MGKKHQVVKFKDIAEKLPELEGKNLEEIAGVLGYRNLESCRVNL
                     YNLRQNKRLGFEVEKGVYTKFELLDDTVKEELEEKELSERGHFLQSLDYYKVAKNAFE
                     IAEDKTVKAETRQKAERDILDAMKHIPKKHRAILYDMMEG"
     gene            1696..2148
                     /locus_tag="VW854_001975"
     CDS             1696..2148
                     /locus_tag="VW854_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263153.1"
                     /translation="MTETTYDIIAKSLDRISMELHQADENNDFLRIGLLSGQLKAIKE
                     NLHRLLWIELPELNESHKIEAVSKSTTGIFFHPGIFEMDAMRQAFFKRQAKHFFDNAT
                     EQQEYIEYAEKEYLEATKTLKDILFNTNNGTRQVNKDCLVEKFEEAMQ"
     gene            2145..2324
                     /locus_tag="VW854_001976"
     CDS             2145..2324
                     /locus_tag="VW854_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263154.1"
                     /translation="MTQSEFYNRMTKIKDRHPNLFQFIIDFLDDKVSTEEVYDFLKME
                     RSYQVNHIKNYKARA"
     gene            2326..2541
                     /locus_tag="VW854_001977"
     CDS             2326..2541
                     /locus_tag="VW854_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263155.1"
                     /translation="MNELDLSNTQAVFVSVVLIGILLYLNHRDRKKSAQIERENQQTI
                     ETPSDDLNPDYGRYIQLAGVRVSGGME"
     gene            2538..2831
                     /locus_tag="VW854_001978"
     CDS             2538..2831
                     /locus_tag="VW854_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263156.1"
                     /translation="MSYTVKIYLDFEKIPDETYFTKEIFLAMCTDNATKLAVTMGLLA
                     KQALDLETRAVILKTLNTIIENQKAILQGVASGQITFLNKSKGEEELDESIRE"
     gene            2812..3153
                     /locus_tag="VW854_001979"
     CDS             2812..3153
                     /locus_tag="VW854_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV0263157.1"
                     /translation="MSLSENDKRVLRLIKVGAENSITGLEISLTTKLTERTVQDIIKR
                     LIIKHNIPIVGVRNGFYRGYFIPRNKGELLDGAKAFYNQVQEESKRLAVLMNSDLESY
                     KETLKEVGGYV"
     gene            3146..3418
                     /locus_tag="VW854_001980"
     CDS             3146..3418
                     /locus_tag="VW854_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263158.1"
                     /translation="MFSLSRESEKDLTHGILEVVERYLEAHEKVPPRLTKLINRIELK
                     EELKISDNTLNKWESQGLRRYQPPDDGSRMIYYLVTDIWKFLGVDE"
     gene            3415..3930
                     /locus_tag="VW854_001981"
     CDS             3415..3930
                     /locus_tag="VW854_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001263812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263159.1"
                     /translation="MRVIELILSADKLPLFGFLKSTPTQVWKNGEHYKFIYFEPIGEG
                     LTAFHYKGLYVAVKDENEEVEGWELTRDLEIGLASPDLLMILKNLEVNKLTEQRQGLG
                     VELKGWVFNLICNGIYTRYETSLFVRLLFVNGYSFSQLVDLFSAIVKRKDLASYFLEV
                     ATKFYKEVAFE"
     gene            3923..5374
                     /locus_tag="VW854_001982"
     CDS             3923..5374
                     /locus_tag="VW854_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated protein E"
                     /protein_id="HEV0263160.1"
                     /translation="MNSNDIVNKIIEENQQEAPPEVVDLTQARKTNEEHNSLNLAKRP
                     RGDGFDRGLDNLRKILSGDSKLKGAIQYNTFTYEIEVTRPMKLNGRTLNGAIDDLIIR
                     EIRAYIATKYKLDYKKPDIADFLEIVAGENSYNPLKDYLESCESEYKELVNQREPFDI
                     LRHYLNIKDDEYNRIIMDLFFRGAVAKVFDPTVKFDFVLDLTGRQGVGKTQFFEGLFT
                     HKYFTTVETFTDKDDKARMVRNWCVFDDEMVASKKASFSELKKFITETKLEFRPPYAS
                     SDRRLPKSFIIVRATNDHDYLNDLTGERRFLVVEVHKDTAYKGRKWTEKDRRAFWGAM
                     VVAWRANQVLNLTDEQEKLVNEVRSRYKFVDEILEDLERYLDTPYPKRMYQFPMTDRM
                     RYYYIYDMMNEGYYRNNRGGTVELDTDTYGELVDRDKMTINLFFQEVYLTDKAPPKDK
                     AKVKKYMQNRDGWEHRRSTRFGKSIKPAYVKKM"
BASE COUNT         1953 a          911 c         1220 g         1439 t
ORIGIN      
        1 tcttttaaag tcaataaatt ctctctacca tcagctgttt ttagttcata gtatgctcta
       61 aatgctttaa aaaatacttc atcagagcca tctttttgac ttaaattttt tacagattca
      121 ataaaattgt tttcataacc gagtaagtta gcaacgctta ccccgaagta atcagcaagt
      181 agttgggctt tttctggttt aatttggctt tctccgtttt ccattcgttg gaatgacctt
      241 aaagaaaatc ccaaagtttc agctatttct ttttgagata gctttttttc ttgtcttaat
      301 tctttcaatc tattcatgtt ttatcacacc tttcacagat gattataacc ttatgtttaa
      361 aaaaagtcaa aaaatgacgt aaaaaaatca aaaaaactat tgacaacgtc taaaactgac
      421 gttataataa aaatcaagct tatgttgcgt cataaaatga cgtcaagcac cccctccaac
      481 ctttcacact ttcaatctat tcatggaggg ggatttttta gaaaggagca aatctatgag
      541 caaactacga ggctatcggg ttatgttagg actaactcag caacaaatgg cggacaagct
      601 aaaaatttct ttgcagtcgt acaacaacaa agaattaggt aaaacgccat tcaatgacaa
      661 agaacgtcta gcgattaagt caatggttgc agaaatcaaa ccagacataa caatagacga
      721 aatattttat agttagaaag gagcgaacta atcgcaatac tactctatat ttacagattt
      781 ctcatgtggt gctttaccac tggggattga caacaaaaaa gtcacttgct gaatgtttgg
      841 cgacagaagc aagcgactgg atcaagagta tagatatttt ttctatacct tgattatagc
      901 atagaaagac aaggtaaaca atgggaaaga aacatcaagt agtaaaattt aaagatatag
      961 ctgaaaaatt gcctgaatta gaaggtaaaa atttagaaga aatcgctgga gtattaggct
     1021 accgcaactt ggagagttgc agggtcaacc tttacaatct cagacaaaac aagcgtctag
     1081 ggtttgaagt agaaaaaggg gtttacacta agttcgaact cttggacgat actgtaaaag
     1141 aagaactgga agaaaaagaa ttgtctgaac gtggtcattt tttacagtcg ctagactact
     1201 acaaagtagc aaaaaacgcc tttgaaatag cagaagataa aacagtaaaa gcagaaaccc
     1261 gacaaaaagc agagcgtgac atattggacg cgatgaagca cattcctaaa aaacatcgcg
     1321 ctatacttta tgacatgatg gagggctagg atggaagcaa aaacccactt cgctagattc
     1381 atgcgtagag gcatggagtt agcaaggcaa ttacatagta aagaaattca acgtgatgaa
     1441 tttgatagag cctggaaaag attaggcgat taaatcgaaa acgaaacgaa gaaaaactaa
     1501 gaaaaccgaa gagcaggcaa gcaattagaa aaggttttga aatcgagtgc tgacacgacg
     1561 attctaagca cttgtttaga aaaaatgtgg gtgattaccc acgaaacatc actacaagcg
     1621 tccgccaact tggggcaaat gcccagcgtt tggagtggtg aaataatcca atataggaaa
     1681 aagggaaaac agactatgac agaaacaaca tacgatatta tcgctaaaag cttggataga
     1741 attagtatgg aattacacca agcagacgaa aacaatgatt ttttgagaat aggactctta
     1801 tcaggacaat taaaagctat caaagaaaat ttacaccgct tactttggat tgagcttcct
     1861 gaattgaatg aaagtcataa aatcgaggca gtctctaaaa gtactactgg aatctttttc
     1921 catccaggta tttttgagat ggatgcaatg agacaagcat tctttaaacg tcaagccaag
     1981 catttttttg acaatgcaac agagcaacag gagtatatag aatatgctga aaaggagtat
     2041 ttagaggcta ctaaaacctt gaaggatatc ctttttaaca ctaataatgg gactagacaa
     2101 gtaaataaag attgtcttgt agaaaaattt gaagaggcaa tgcaatgaca caatcagaat
     2161 tttacaaccg aatgactaaa atcaaggata gacatccgaa cctctttcag ttcatcattg
     2221 actttttaga tgataaagta agcaccgaag aggtgtatga ttttctgaag atggagcgaa
     2281 gctatcaagt aaatcatatc aagaattaca aagcgagagc ataacatgaa cgaactagat
     2341 ttaagcaaca cacaggcggt ttttgttagc gtggtactga ttggcatact gctttatcta
     2401 aaccaccgag accgcaaaaa aagcgcccaa attgagcgag aaaaccaaca gacgatagaa
     2461 acacctagcg atgatttaaa ccctgattat gggcgatata tccagcttgc aggggtaaga
     2521 gtttccggag ggatggaatg agttacacag tgaagatata ccttgatttt gaaaaaatac
     2581 cagatgaaac ctattttact aaagagattt ttctagctat gtgtacggac aatgcaacaa
     2641 agttagctgt aactatgggt ttgctggcaa aacaagcgtt ggatttagag actagggctg
     2701 ttattttaaa aacccttaat acaatcatag aaaatcaaaa agctattttg caaggggtag
     2761 cgagcggaca aataaccttt ttgaataaaa gtaaaggaga ggaggaactg gatgagtcta
     2821 tcagagaatg acaagcgagt tttaagacta atcaaggtag gggctgagaa ctccataaca
     2881 gggttagaaa tcagcctgac aaccaagcta acagaaagaa ccgtgcaaga tattattaag
     2941 cgtttaataa tcaagcataa catcccaatt gtgggggtta gaaatggatt ttatagaggt
     3001 tatttcatac ctagaaacaa aggcgagtta ctagatggtg ctaaggcttt ttacaaccaa
     3061 gtacaggagg agagcaagcg cctagcggtg ttgatgaata gcgatttaga aagctacaaa
     3121 gaaaccttga aggaggtggg cggatatgtt tagcctaagc cgagaaagcg agaaagacct
     3181 aacgcatggc attctggagg tagtggaaag atacctggaa gcgcatgaga aagtaccgcc
     3241 aagattgacc aagttaataa ataggattga actcaaagag gaattgaaaa ttagtgacaa
     3301 cacgctgaat aagtgggaaa gtcagggttt aagacgctat caaccgccgg acgatggttc
     3361 aagaatgatt tactatttag taactgacat ctggaagttt ctgggggtgg atgaatgagg
     3421 gtgatagagt taattttatc cgctgacaaa ttgcctttgt ttggctttct caagtctacg
     3481 ccaacccaag tttggaagaa tggggaacac tacaaattta tctattttga gccaataggc
     3541 gagggcttga cggctttcca ctacaaaggt ttgtatgtgg cagttaaaga cgaaaacgag
     3601 gaagtagagg gctgggaact aaccagagat ttagaaatag gtttggctag tcctgacttg
     3661 ctgatgatcc tgaaaaattt agaggtaaat aaattgacag agcaacggca ggggcttgga
     3721 gtggagttaa aaggctgggt ttttaacctg atttgtaacg gcatttatac cagatatgag
     3781 acttcgcttt ttgtccgctt gctatttgtc aatggctaca gttttagtca gctggtggac
     3841 ttgttttcag caatcgtcaa acgcaaagac ctagcaagct actttctaga agtagcgaca
     3901 aaattctata aggaggtggc ttttgaatag caatgacatt gtaaataaaa tcattgaaga
     3961 gaatcagcaa gaagcaccgc ctgaagtggt ggacttgacc caagcaagga aaaccaacga
     4021 ggaacacaat agcctgaact tggcaaagag accaagaggg gacggctttg atagaggttt
     4081 agacaatctg agaaagattt tgagcggaga tagcaagcta aaaggggcga tacagtacaa
     4141 cacttttaca tatgaaattg aagtgactag accaatgaag ctaaacggta gaaccctgaa
     4201 cggtgcaatc gatgacctga ttatcagaga gattagggct tatattgcta ccaagtataa
     4261 gctagactat aaaaagcctg atatagctga ttttttggag atagtggctg gagaaaatag
     4321 ctacaacccc ttaaaagact atctggaatc ttgcgaaagc gagtataaag agttagtgaa
     4381 tcaacgtgag ccctttgata ttttaaggca ttatcttaat atcaaggatg acgaatataa
     4441 ccgtattatc atggatttgt ttttccgtgg agcggttgcc aaggtgtttg accctaccgt
     4501 taagtttgac tttgtgctgg acttgactgg aaggcaggga gtaggaaaga cacaattttt
     4561 tgaggggctt tttactcaca agtattttac aaccgttgag acattcacag acaaagacga
     4621 caaggctaga atggtgagaa actggtgtgt ctttgatgat gagatggtgg ccagtaaaaa
     4681 ggctagtttt tcagaattga agaaattcat cacagaaacc aagctagagt ttagaccccc
     4741 ttacgcttcc agtgacaggc gattgcctaa gagttttatc attgtcagag caactaatga
     4801 ccatgattat ttgaacgacc tgacagggga aaggcgcttt ctagttgtag aagtccacaa
     4861 ggacaccgcc tataagggca ggaaatggac agagaaagac cgcagagcct tttggggcgc
     4921 tatggtagta gcttggaggg ctaaccaagt cttgaacctg acggacgagc aggaaaagct
     4981 agtaaatgag gttagaagtc gttacaagtt tgtagatgaa attcttgagg atttggagcg
     5041 ctatttagat actccttacc caaaaagaat gtatcagttc ccgatgaccg acagaatgcg
     5101 ctactattat atctatgata tgatgaacga gggatactat agaaataaca ggggtggaac
     5161 agttgaactt gacacagaca cctatggcga actggtagat agggacaaga tgaccattaa
     5221 cctatttttt caagaggtgt atttgactga caaagcaccg ccaaaggata aggctaaggt
     5281 taaaaagtat atgcaaaacc gagacggctg ggaacatagg cgatcaacaa gatttggtaa
     5341 aagtattaaa ccagcttacg ttaaaaaaat gtagtcagtg tagtttattt ttaaaagtga
     5401 ctacatttta aacaggaaga aaacccttga tacataaggg gttagaaaaa agtgttgttt
     5461 ttactatttt actgactaca cccctaaacc cttggtatta ttgactttta tataaaaatg
     5521 tag
//
LOCUS       DAVKWZ010000090         5493 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000090 DAVKWZ010000000
VERSION     DAVKWZ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5493
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.090"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            82..1836
                     /locus_tag="VW854_001983"
     CDS             82..1836
                     /locus_tag="VW854_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0263161.1"
                     /translation="MKIYKKLFAYVQDKKYLGVLAIIFSAISALLTVYGYYLIYKFLD
                     KLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKA
                     SFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIITPVLVLALGFIVSIRVGI
                     ILLALTIIGGLILGAMMGEQEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFK
                     SFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLE
                     LIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQKDKLVHGNINNFKNY
                     NIEFENVSFAYNDKAVIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSI
                     KIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGC
                     DLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEF
                     ELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSL
                     QEMFNSANEWRVSNERVL"
     gene            1820..3541
                     /locus_tag="VW854_001984"
     CDS             1820..3541
                     /locus_tag="VW854_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0263162.1"
                     /translation="MKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAGNVKMGL
                     AVIIPSILSFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     DDLYKKMEDSERIHLKAEVTTILTLSISSIFSFISLAVVIFVGVNLIINKEINSLYLI
                     GYLLAAMKIKDSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKFDIDLKD
                     VEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQILIDGK
                     DIKEISTESLFDKVSIVFQDVVLFNQSVMENIRIGKQDASDEEVKRAAKLANCTDFIE
                     KMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVNNEKKIQES
                     LNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKNLIEKTK
                     MAEEFIY"
     gene            3552..4136
                     /locus_tag="VW854_001985"
     CDS             3552..4136
                     /locus_tag="VW854_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEV0263163.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     YMALVALGAFLGGILGAYIGKALLKKHFEKAGIV"
     gene            4136..4831
                     /locus_tag="VW854_001986"
     CDS             4136..4831
                     /locus_tag="VW854_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV0263164.1"
                     /translation="MPTFSSNAPFNPNPISKILVVFITGLTVMHSINIRFELAIVCLI
                     GILLYLNGYKKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIPIVIRMFILPFMA
                     ASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKNIKIAMRVRGINF
                     KNPVKYLEYVSVPLLIISSNISDDIAKAAETKAIENPIAKTRYIRVKIQLIDFVYVLA
                     VVGLIVGGLIWLK"
     gene            4819..>5493
                     /locus_tag="VW854_001987"
     CDS             4819..>5493
                     /locus_tag="VW854_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0263165.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGSGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDMLEIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDESSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMDIVDRVFLIDKGKLK
                     KTYTRSE"
BASE COUNT         1971 a          717 c         1030 g         1775 t
ORIGIN      
        1 tataatagaa aagcaatgat agatattatc aattaagcga atttatatct aaaagggata
       61 ttaaagaaag gagatatgct tatgaagatt tacaaaaaac tatttgctta tgtccaagat
      121 aagaaatatc ttggggtttt ggccataatt ttttctgcta tatctgctct acttacagta
      181 tatggatatt atttaatcta caaatttcta gataagttaa taattaattc aaatttatcc
      241 ggtgcagaga gcatagcatt aaaatctgtt attacactaa caagtggagc gatattttat
      301 tttgtctcag gaatgttttc acatatcttg ggattcaggc ttgaaacaaa tttaagaaaa
      361 aggggaatcg atggtctgga aaaagcaagt tttaggttct ttgacttaaa tccatctggt
      421 caaataagaa agattataga tgacaatgct gcacaaactc atcaggtggt agcacacatg
      481 attcccgata gttctcaggc aataatcaca cccgtacttg tacttgcact tggctttata
      541 gtaagtataa gagttggcat aattttgctt gctcttacta taattggtgg cttaatttta
      601 ggggcaatga tgggcgagca agaatttatg aagatatacc aagaatccct atctaaacta
      661 agtgctgaaa ctgttgagta cgtgagagga atgcaagttg taaaaatatt taaagcaaat
      721 gtagagtctt ttaaaagctt ttataaggcg ataaaagatt actcaaagta tgcttatgat
      781 tattccctat cttgtaaaag gccttatgtt ttgtatcaat ggttattttt tggactgatt
      841 gcaattttaa ttattcctat agtttatttt atgactagct tagctagcgc aaaggtgatt
      901 ttacttgagc ttatcatgat tttattttta tcaggagttc tctttgtttc attcatgaga
      961 atgatgtggt actccatgta tatttctcaa ggaaattatg cagtagatac tttagaggcg
     1021 ctttacgaag atatgcaaaa agacaaatta gtgcatggta atatcaataa ttttaaaaac
     1081 tataatatag aatttgagaa tgttagcttt gcttataatg ataaagctgt cattgaaaat
     1141 ttatccttta atttagaaga aggaaagtcc tacgcacttg tcggttcatc tggatcaggc
     1201 aaatcaacag tagcaaaact tatatcaggt ttttataatg ttaataaagg aagcataaag
     1261 ataggcggga tagcaataag tgaatattct gacgaagcct taattaaagc catttccttt
     1321 gtttttcaag attcaaaatt attcaagaag agcatttatg ataatgtagc tttagctaat
     1381 aaagatgcga cgaaagatga cgttatgaga gccttaaaat tagcaggatg cgatttaata
     1441 ttagacaaat tcccagaaag agaaaataca atcataggct caaaaggtgt ttatttatcc
     1501 ggtggagaaa aacaaagaat tgcaattgct agagcaattt taaaggattc caaaattatt
     1561 attatggatg aagcatcagc atctattgac ccagataacg agtttgaatt gcaaaaagct
     1621 tttaaaaatc ttatgaagga taaaacagtt atcatgattg cacacaggct atctacaatt
     1681 aaagaccttg atgaaattat tgtcatggat agtggaaaaa ttatagaaag agggtctgac
     1741 aaagaattaa tgtcaaaaga tacaaggtat aagagcctgc aagagatgtt taacagtgcg
     1801 aatgaatgga gggtttcaaa tgaaagagtt ttataaaaaa agatttgctc ttacagatgg
     1861 aggagcaaga aatttaagta aagcaacact ggcttcattt ttcgtttatt gtataaacat
     1921 gcttcctgcc atattactta tgatttttgc tcaggaagtt ttggaaaata tgggcaaaag
     1981 caatggcttt tatatagtat tctcagtttt gactttgata gcaatgtata ttttgctttc
     2041 tatcgaatac gataaattat ataacacaac ctatcaagaa agtgcagatt taagaataag
     2101 gacagcggag aatttatcaa aattacctct atcttacttt tctaaacatg acatttccga
     2161 catttcacaa acaatcatgg ctgatattga aggcatagag catgcaatga gccactcaat
     2221 accaaaggtg ggcggcatgg tactgttttt cccattaata tctgtaatga tgctagcggg
     2281 caatgtcaag atgggtttag ctgtaattat tccatctatt ttaagcttta tatttatacc
     2341 tttatctaaa aaatatcagg ttaatggaca gaatagatat tatgatgtct taagaaaaaa
     2401 ctcagaaagc tttcaagaaa atatcgaaat gcaaatggag attaaagcat ataatttatc
     2461 gaaggatatt aaagacgact tatataaaaa aatggaagat agtgagagaa tacacttaaa
     2521 ggcggaagta actacaattt taactttgtc tatatcttca atatttagct ttatatctct
     2581 tgctgttgtg atatttgtcg gcgtaaatct aattattaat aaagagataa attctctcta
     2641 ccttatagga tatttactag ctgctatgaa gataaaagac tctttagatg catctaaaga
     2701 gggcttgatg gaaatatttt atttatcgcc caaaatagaa agattaaaag aaattcaaaa
     2761 tcaagattta caagaaggcg atgactatag cttaaaaaaa tttgatattg atctaaaaga
     2821 tgttgagttt gcctacaata aagacgcaaa agttttaaat ggtgtcagtt ttaaagctaa
     2881 gcagggagag gtcactgctt tggtaggtgc aagtggctgc ggtaaaacaa ctatcttgaa
     2941 acttatatca agactttatg attatgacaa gggacaaatc ttaatcgatg gcaaagatat
     3001 aaaggaaata tcaacagaat ccctttttga taaggtgtct attgttttcc aagatgtggt
     3061 tctctttaat caaagcgtta tggaaaatat tagaatcggt aagcaagatg caagtgacga
     3121 agaggttaaa agagcagcaa aacttgcaaa ttgcacagat tttatagaaa aaatggataa
     3181 aggtttcgat acagttattg gtgaaaacgg agctgagcta tcaggaggag aaagacaaag
     3241 attatcaata gccagagcct tcttaaaaga tgcgccgata ttgatcttag atgagataac
     3301 agcaagcctt gatgttaaca acgagaaaaa gattcaagag tctttaaata atttagttaa
     3361 agataaaact gttgtaatca tttcacatag aatgaaatcc atagaaaatg cagacaagat
     3421 agtagttctt caaaacggaa gagtagaaag cgaaggtaag catgaagagc ttttacaaaa
     3481 atcaaaaatt tacaaaaatt taatagaaaa gacaaaaatg gcagaagaat ttatttatta
     3541 ggaggactac aatggataat aaaaaattaa aagtaaaaga tttagtaagt atcggtgttt
     3601 ttggcgtaat ttattttgcc ttcatgtttg gagttggtat gatgggcttg attccaatat
     3661 tgttcttaat atacccgaca gtattagcca tagttgcagg aactgttgtt atgttattta
     3721 tggctaaggt tcaaaagcca tgggcactat ttatatttgg tatgatatca ccacttgtga
     3781 tgtttgcagc tggtcatacc tacgtagttg tggttttatc acttatagta atgataatag
     3841 cagaattaat tagaaagatt ggtaattata attcatttaa atacaatatg ctttcttatg
     3901 caatcttcag cacatggata tgtagctctt taatgcaaat gcttttagca aaagaaaaat
     3961 atatggagtg gtctttgatg acgatgggaa aagattatgt tgatgtatta gaaaagttaa
     4021 taacttatcc ttacatggct ttagtagcct taggtgcttt cttaggagga attcttggag
     4081 catatatagg caaggctcta ttgaaaaaac actttgaaaa agcaggcatc gtataatgcc
     4141 tactttttct tcaaatgctc cctttaatcc caatccgatc tctaagatat tagtcgtatt
     4201 tatcacaggc ttaactgtta tgcatagcat aaacatccgt tttgagctgg caattgtttg
     4261 tcttattggt attttgcttt atttgaatgg atacaaaaaa acacttttca aatggattat
     4321 attgtgtggg atactttact ccttgcctaa ttttatggtg ttatctgaat taaaccctat
     4381 agttaagatg tttttgagta tacctattgt tattagaatg tttattttac catttatggc
     4441 agcaagcttt atgataaaga cctcggatgt aggcgcaata atttcatcga tggataagct
     4501 taagatttca aagaatgtat ccatacctat tgcggttatg tttagattct tcccatcttt
     4561 taaggaggag aagaaaaaca tcaaaatagc tatgagagta agagggataa attttaaaaa
     4621 cccagtcaaa tatcttgaat atgtttctgt gccactactc attatatcat ctaatatatc
     4681 agatgacatt gcaaaagcgg cagaaacaaa ggcaatagaa aatccaattg ccaagaccag
     4741 atacattcgc gtaaagatac agctaattga ttttgtttat gttttagcgg ttgttggact
     4801 tattgtggga ggcttaatat ggttgaaata aaaaatttaa gtcttgatta tggtgaagag
     4861 catatattag atgatatatc actatccata gccgagggag agtgcgtgct atttacagga
     4921 aaaagtggaa gtggtaagtc atctttaata aattcaatca atggactagc tgtaaggtat
     4981 gataacgcaa agacaaaggg cgaaataatt attgatggta agaatataaa aaatttggaa
     5041 ctttatcaaa tctcaatgct tgtttcaact gtttttcaaa atcctaagac atattttttt
     5101 aatgtcaata cgacattaga attattattt tatttggaaa atatcggtct tgcaagagaa
     5161 gagatggaca ggcgtttgaa ggatatgctt gagatattcc cgataaaaaa tcttttgaac
     5221 agaaatatat ttaatctatc cggcggtgaa aaacaaattc tttgcattgc agcttcttat
     5281 atagcaggta caaagattat agttatggat gagtcttcat cgaatttaga tattaaaagc
     5341 ataagtgttt tggcaaagat gctaaagata ttaaaagaga aaggcataag cataattgtt
     5401 gcagagcata gaatttatta tttgatggac atagttgacc gtgtattttt aatagataaa
     5461 ggaaagctta aaaaaactta tactagaagt gaa
//
LOCUS       DAVKWZ010000091         5335 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000091 DAVKWZ010000000
VERSION     DAVKWZ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.091"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            148..870
                     /locus_tag="VW854_001988"
     CDS             148..870
                     /locus_tag="VW854_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV0263166.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTC
                     QPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            1088..2422
                     /gene="pepC"
                     /locus_tag="VW854_001989"
     CDS             1088..2422
                     /gene="pepC"
                     /locus_tag="VW854_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEV0263167.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSR
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQATVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
     gene            complement(2478..3389)
                     /locus_tag="VW854_001990"
     CDS             complement(2478..3389)
                     /locus_tag="VW854_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV0263168.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(3413..4216)
                     /locus_tag="VW854_001991"
     CDS             complement(3413..4216)
                     /locus_tag="VW854_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEV0263169.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(4244..5233)
                     /gene="manX"
                     /locus_tag="VW854_001992"
     CDS             complement(4244..5233)
                     /gene="manX"
                     /locus_tag="VW854_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEV0263170.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
BASE COUNT         1630 a         1089 c         1163 g         1453 t
ORIGIN      
        1 tacaaaaata agaaataaat agattaacca catatggatg tttcctttta aacgaagtat
       61 atttatacta taccataaaa ttgataatag ttcacctaat aaaatgttag ttatgtacaa
      121 caataatttc catgatataa tagttctatg agattagata aatttttagt tgcctgtgcg
      181 gtgggaagtc ggactgaggt caaaaacttg ctcaaggctg ggcgcgtgac tgtaaatggt
      241 aaaaaagaaa aatcagctaa attgcagatt gatgaaaaaa tagatgagat tcgctttgat
      301 gggcaagtgt tggagtatga agagtttgtc tactacatga tgaacaagcc caaaggagtt
      361 atctcagcga ctgaggatcc caagcacaga accgttctgg acttgttgga tgacttggcg
      421 cggagtaagg aagttttccc agtaggacgc ttggatattg acacgcatgg tcttttgctc
      481 ttgaccaatg atggtcagct ggctcatgtt cttctttcgc ccaagcgtca tgtggacaag
      541 acttatctgg cacaagtcaa gggaatcatg acccaagaag atgtggagac atttgctgag
      601 ggtattcctc tcaaagactt tacctgtcaa cccgctatac tggagcttgt atccatagat
      661 acagaaaaga atcaaagcca aatccgtgtg accattgcag aagggaagtt tcatcagatc
      721 aagcgtatgg tgggctactg tggcaaggaa gtggtggact tgcaacgatt gactatggga
      781 actttagtat tagatgagaa cctggaacga ggggaatggc gtcgcttgac caaggaagaa
      841 ttagaaattc tccgcgctaa tattatttaa tttagtttaa tactcaatga aaatcaaaga
      901 gcaaactagg aaactagccg caggctgtac ttgagtacgg caaggcgacg ttgacgtggt
      961 ttgaatttga ttttcgaaga gtataaattc gaaaaaggtg agggacaatg tcctcgcctt
     1021 ttatgttttt tagttgcttc ctttgtgaaa agagttataa tagactgtag aataaaagga
     1081 ggaatctatg aacgcgattc aagaatcatt tactgataaa ctatttgcca actatgaagc
     1141 aaatgtaaaa taccaagcga ttgaaaatgc tgccagccac aacggaattt ttgcagctct
     1201 ggaacgtcgc caaagccgtg tagacaacac acctgttttc tcattggatt taaccaagga
     1261 caaggtcact aaccagaaag cgtctggtcg ttgctggatg tttgcggctc tcaacacctt
     1321 ccgccacaaa ctcatctcgc aatacaaatt ggagaacttt gagttgtcac aagcccacac
     1381 tttcttctgg gataagtatg agaaatcaaa ctggttcttg gagcaagtca ttgcgacttc
     1441 agaccaagaa ttgactagcc gcaaggttag cttcttactc caaacacctc aacaagatgg
     1501 cggtcaatgg gatatggtcg tttccctctt tgaaaaatac ggtgtcgtgc ctaagtcagt
     1561 ttatcctgag tctgtttcat ctagcagcag tcgtgagcta aatgcgatcc ttaataaatt
     1621 gcttcgtcaa gatgctcaaa tcttgcgtga cttgcttgtt tctggtgcag atcaagcgac
     1681 tgttcaagct aagaaagaag acctcttgca agaaatcttt aactttcttg ctatgtcatt
     1741 aggacttcca ccacgcaagt ttgactttgc ttatcgcgat aaagataaca actacaaaag
     1801 tgaaaaagga atcacaccac aagagtttta caagaaatat gtcaatcttc ctttagaaga
     1861 ctacgtttct gttatcaatg ctccaactgc tgataaacct tacggaaaat cttacacagt
     1921 tgagatgttg gggaatgttg ttggtagccg tgcagttcgc tacatcaacg ttccaatgga
     1981 gcgcttgaaa gaattggcga ttgcccaaat gcaagcaggt gagactgttt ggtttggttc
     2041 tgatgtcggc cagctcagca accgtaaggc tggaatcctt gcgacagatg tttatgactt
     2101 tgaatcaagc atggacatta aacttactca agacaaggct ggacgtttgg actatagcga
     2161 aagcttgatg acccacgcca tggtcttgac aggtgttgac ttggacgaaa atggtaaatc
     2221 aaccaagtgg aaggttgaaa actcatgggg agacaaggtc ggtacagatg gttactttgt
     2281 tgcctcagac gcttggatgg acgaatacac ataccaaatc gttgttcgta aggaattgct
     2341 gacagcagaa gaacaagctg cctatggagc agaaccaatc gtacttgcac catgggatcc
     2401 aatgggagcc ttggctgaat aaaagcatag aaaaaaggaa tcagatttta gaacctggtt
     2461 cctttttagt tgcttgatta catgatgtga agaacatgtg ccacaatacc cactgcgaag
     2521 agggcaagga taatagtgat tggagatact tttttcttaa gtaagtacat gcaaagtaaa
     2581 gtaaggagta gtcctgataa tccaggaatc aacatatcca agttttgttg gaaagtagta
     2641 actttttcag gagtttgaga caatccttgt cctacttgtg cgaatgcttc ttggatacct
     2701 ttagaccctt ctggcaattt atcccaatgg atataagcct tttcatctag ttgaacttta
     2761 gaaacatcga aagcaaattt aatatttacc caacgttgaa caaggacagc aagaatgaac
     2821 atcccaagga tagaagctcc tttagtgata tcttgaagga taccaccaga catatcttta
     2881 gtgatttctg atccagcctt gtatccaatc tcttgaacat accacaagaa tgacatacga
     2941 atcaagttcc atgcaacaaa gaagaggagt ggccccaaga tattgccagt aagggcaagt
     3001 gaagcaccga gagatccaag gattgggcgt actgtaaacc agaatactgg gtcaccgata
     3061 ccagcaagag gtcccatcat accgattttg accccttgga tagcagcgtc atcgatttcc
     3121 acaccgttag cacgttcttc ttcaagcgca agagtaaccc ccatgactgg agcagctacg
     3181 tatggatgag tgttgaagaa ctcaaggtga cgctcaagag cagcgatttg atcttctttt
     3241 ttagtataga gttttttgat agctggaatg agtgtataag cccagcccaa gttttgcatc
     3301 cgttcaaagt tccaagaccc ttgtaagaag gttgaacgcc accaaacttt tttacgatct
     3361 gatttagtta attgaagttt ttcagtcatg atgttttcag tcctttctta tcttagtagt
     3421 cttctaggat atcgccgatt gggtcgttag aagttgcggc tcctccgcca ccatttccac
     3481 cagttttaga aaggtgaagg tagataagag cgatagcaac accgatagca ccgaatccga
     3541 ttagagtaat atctgacaca gcagcgagaa cgaaaccaag agcgaagaat ggccatactt
     3601 cacgagttgc catcatgttg ataaccatgg cgtaaccaac ggcaacgacc ataccaccac
     3661 cgatagccat accatctttg agccagtctg gcatggcact aaggatactt tgtacagttt
     3721 cagttggtac cataaggaga agagctgcag gaagcgcgat acgaagtcct tggaagagta
     3781 gcgcaatgaa atgcgcacgc tccacagcgc cgaagtcacc ttttttagcg gcagcatctg
     3841 cagtatgaac caaaccaact gaaattgtac gaacaatcat tgtcaagaaa agtccagcta
     3901 cggcaagagg gatagcaacc gcttgggcaa caccgatacc agtcttggta aagtcaccac
     3961 caagaaccat gataatggca gcagcgacag aagcaagtgc agcatcagga gcgatagcag
     4021 caccgatatt tgaccaacca agggcaatca tttgaagcga tccaccgagg ataatccctg
     4081 cttccaagtg acctgttaca agcccaataa gggtacaggc tacaagtggt tggtgaaatt
     4141 ggaactggtc gaggatgcct tcaagacctg caaagaaggc tacaacgact actaaaacca
     4201 tagaaataat agacatgttt aaaatccttt cataaataat ggcttatttg acattggctt
     4261 tgttaatcaa gtcaaacaaa tcttttttag aatcatttgg tactttacgg acatcaaatt
     4321 caacacccaa gtcacgcatt ttttcaaatg tcgcaacatc ttctttgtcc atagacaaaa
     4381 cggtattgac caatgtttta cctgttgagt gagccataga accaacgtta agagtcttga
     4441 ttggcacgcc gccttcgatg gcacgaaggg catcttgagg tgtttcaaac aagataaggg
     4501 catgtgtttc tccaaaacgt gggtcttttg aaatctcaat cagtttttga attggaacca
     4561 cgttagcctt gacattacct ggagctgctt gtttaatcaa ttctttacga aggtcgtctt
     4621 tagccacatt atctgaagca acgatgatac gatttgcttt tgaatctgga gtccaagcag
     4681 ttgcaacctg accgtgaagt agacgtgtgt caagacgggc aagattgatt ttcaatttac
     4741 cgtctccgat aacagttcct tctgggatag cagtttgggc aactggagca gctgcagcgc
     4801 ttgcaacttc ttcaactgga tttagctctt ctggaagagc tttgatgcca tctttggctt
     4861 ctttaatgat attagcagcg actttttcta cacctgcagc agcgtccatg aggcgctctg
     4921 tgtaggcttg aatcaacatc ggtaagttaa gtcctgtgat gatggcaaac ttacgctcag
     4981 gattttctcc catcacgcga ctagcttggt taaatggaga accactccaa aggtcagcca
     5041 aaactagaac ctcatcttct gcgtcaaatg cagcaacagc gttattaaac ttagcgtata
     5101 gatcatcagg accttcattt ggcataaagg tcacaacttg aaccttttct tgttcaccaa
     5161 agatcataga tcctgactga tgaatacccg cagcaaattc gccgtggctc gcaataatga
     5221 ttccgatact cattattgtc attcctcctt ttttgtttta gttttcattt aagaaaactt
     5281 taagactgtt taagtataaa ccgttttcat aaaaaaagca actatttatc ataaa
//
LOCUS       DAVKWZ010000092         5213 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000092 DAVKWZ010000000
VERSION     DAVKWZ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.092"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            192..1571
                     /locus_tag="VW854_001993"
     CDS             192..1571
                     /locus_tag="VW854_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV0263171.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVIAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            1723..2598
                     /gene="pdxS"
                     /locus_tag="VW854_001994"
     CDS             1723..2598
                     /gene="pdxS"
                     /locus_tag="VW854_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEV0263172.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            2599..3183
                     /gene="pdxT"
                     /locus_tag="VW854_001995"
     CDS             2599..3183
                     /gene="pdxT"
                     /locus_tag="VW854_001995"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEV0263173.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEDVEILATVDNQIVA
                     AQEKNMLVTSFHPELTDDAHLHQYFIEMCKEKRQ"
     gene            3740..4471
                     /locus_tag="VW854_001996"
     CDS             3740..4471
                     /locus_tag="VW854_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0263174.1"
                     /translation="MVNNVAVKVSNLSKEFLLGQDKTVSILKDISLSVNYGEFVSILG
                     VSGSGKSTLLSCLSSLSEPTSGEVVINGVNPYTLKEGKLAKFRRQDIAIIFQNYNLVP
                     ALPVLENVTLPLRLSGKSVDSNKVKKMLDSLNFKAELSSLVATLSGGEQQKVAITRAI
                     IADSKIIFADEPTGALDSVSRKLIFETLRNLASQGKCVLMVTHDIELASKTDRALILK
                     DGKISRQIIKPSADELYQALESSKD"
     gene            4489..>5213
                     /locus_tag="VW854_001997"
     CDS             4489..>5213
                     /locus_tag="VW854_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-carotene 15,15'-monooxygenase"
                     /protein_id="HEV0263175.1"
                     /translation="MLKLIIYQFQYSKRQWLGTIPLLFVSSLIVETSLFGIASSIKTA
                     NINASQLFQMLIIFGGTTLFFLISNNIRLLIDIFKKDYQLWTILGASRTQLSLLVSGQ
                     FYLMAVIVSSIGTILSFIMADSYYKFLQNLLGRDELPDLVITANIQSILLSIFIVPTI
                     VGIGAYFYSSRILKISSILKPKKKKRKVTVTGFVNISVRLFLWLLCIGSIVSAGFIRN
                     KEIIEKQSSIILFLLIIHILIIQS"
BASE COUNT         1669 a          879 c         1055 g         1610 t
ORIGIN      
        1 ttagaatttt cacaaacaaa atttggaaaa agtcaagaaa tatgctcata aaattcatga
       61 ggcttgaaaa caggataaat ggggaattat ttttgataaa aaatgctgaa ataatagtac
      121 cccccttgta aacgctaacg gtaaatggta tactagtaaa gtaaatttag aatgaaggca
      181 ggaaattttt tatgagtaaa atcgttgtag tcggtgctaa ccacgctggt acagcatgta
      241 tcaataccat gttggataat tttggaaatg agaacgaaat tgttgtattt gaccaaaact
      301 ctaacatctc ttttctagga tgtggaatgg ctctttggat tggtgaacaa attgacggtg
      361 ctgaaggctt gttctattct gataaagaaa aattggaagc taaaggtgct aaagtttaca
      421 tgaactcacc tgttctttca atcgactatg ataacaaagt agttatagcg gaagttgaag
      481 gaaaagaaca caaagaatca tacgagaaat tgattttcgc tacaggttct acaccaatct
      541 tgccaccaat cgaaggtgtt gaaattgtta aaggaaaccg cgaatttaaa gcaactcttg
      601 aaaatgttca attcgtgaaa ttgtaccaaa atgctgaaga agttatcaat aaactttctg
      661 acaagagcca acacctcgac cgtatcgccg ttgttggtgg tggttacatc ggtgttgaac
      721 ttgctgaggc ctttgaacgt cttggaaaag aagttgtcct tgttgatatc gttgatactg
      781 tcttgaacgg ttactatgac aaagacttca cacaaatgat ggcgaagaac ttggaagatc
      841 acaacatccg cttggctcta ggtcaaactg ttaaagcaat cgaaggtgac ggtaaagttg
      901 aacgcttgat tactgacaaa gaaagctttg acgtggatat ggttatcctt gcagttggtt
      961 tccgtccaaa cacagccctt gctgatggta agatcgaact cttccgcaac ggtgccttcc
     1021 ttgtagacaa gaaacaagaa acatctatcc caggcgttta cgctgttggt gactgtgcga
     1081 ctgtttatga caatgctcgt aaagatacaa gctatatcgc tcttgcttca aatgctgttc
     1141 gcactggtat cgttggtgcc tacaacgctt gtggacatga attggaagga atcggtgttc
     1201 aaggttcaaa tggtatctca atctacggtc ttcacatggt ttcaactggt ttgactcttg
     1261 aaaaagcgaa agctgctggt tacaacgcaa ctgaaacagg ctttaacgat cttcaaaaac
     1321 cagaattcat gaaacatgac aaccatgaag tagcaattaa gattgtcttt gacaaagata
     1381 gccgtgaaat tcttggtgcc caaatggttt cccatgatat tgcaattagc atgggaatcc
     1441 acatgttctc acttgctatc caagagcatg tgacaattga taaattggca ttgacagacc
     1501 tcttcttctt gccacacttc aacaaaccat acaactacat cacaatggct gcccttacgg
     1561 ctgaaaaata aaaatgaatg agctatctgg ccttaagtta aggtcagata gtttttagct
     1621 aatctgtccc catacaatta tagttttttt atcttgtgct tcattctgtt ctgacttaaa
     1681 atgaaaaggt agctaccaat acaaatgatg aggataaaac aaatgactga aaatcgttat
     1741 gaactaaata aaaacttggc acagatgctc aagggtggtg ttattatgga tgtgcagaat
     1801 cctgaacagg ctcgtatcgc agaagctgct ggtgcggcag ctgtgatggc cttggaacga
     1861 attccggctg atattcgtgc agctggagga gtttcccgca tgagcgaccc aaagatgatt
     1921 aaggaaatcc aagaagcggt tagtattcca gtaatggcta aggtcagaat cgggcatttt
     1981 gttgaagctc agattttaga ggctattgaa attgattata tcgacgagag tgaagttcta
     2041 tctccagctg atgaccgttt ccatgtggac aagaaagaat tccaagttcc ttttgtctgt
     2101 ggtgctaagg atttgggtga agccttgcgt cgtatcgctg aaggtgcttc catgattcgt
     2161 accaaaggag aaccagggac aggggatatc gtccaagctg ttcgtcatat gcgtatgatg
     2221 aatcaggaaa ttcgccgcat tcaaaactta cgtgaggacg agctttatgt tgctgccaag
     2281 gatttgcaag tccctgtaga attggtccaa tatgttcatg aacatggaaa attgccagtt
     2341 gtaaatttcg ctgctggagg tgttgcaacg ccagcagatg ctgcgttaat gatgcaacta
     2401 ggggcagagg gggtctttgt cggttcaggt attttcaagt caggagatcc tgttaaacga
     2461 gcgagtgcca ttgttaaggc tgtgactaac ttccgtaatc ctcaaatcct agctcaaatc
     2521 tctgaagatt taggagaagc catggttggt attaatgaaa atgaaatcca aattctcatg
     2581 gctgaacgag gaaaatagat gaaaatcgga atattggcct tgcaaggggc ctttgcagaa
     2641 catgcaaaag tgctagatca attaggtgtc gagagtgtag aactcagaaa tctagatgat
     2701 tttcagcaag atcagagtga cttgtcgggt ttgattttgc ctggtggtga gtctacaacc
     2761 atgggcaagc tcttacgtga ccagaacatg ctacttccca tccgagaagc cattctatct
     2821 ggcttaccag tgtttgggac ctgtgcgggc ttaattttgc tggctaagga aatcacttct
     2881 cagaaagaga gtcatctagg aactatggat atggtggtcg agcgtaatgc ttatgggcgc
     2941 caattaggaa gtttctacac ggaagcagaa tgtaagggag ttggcaagat tccaatgacc
     3001 tttatccgtg gtccaattat cagcagtgtt ggagaagatg tagaaattct agcaacagtt
     3061 gataatcaaa tcgttgcagc ccaagaaaaa aatatgttgg taacttcttt tcatccagaa
     3121 ttgacagatg atgctcactt acaccagtac tttatcgaaa tgtgtaaaga aaaaagacag
     3181 taaaaaagct caaaaaatca cttcctgtgc tatactgtaa ttgaaatttt tttagttttt
     3241 gcgttagaac tgtttcctca gtcctagcgc cttttctttt tgataagtct gcttggtgga
     3301 gagtagtttg taaacgattc tgagaatctt atgagcacaa gcaatgacgg ctttcatctt
     3361 gcttcctctt tgggaaattc gattgtaaaa ctctaagcag gctctgcctg cttagagttt
     3421 tacatatgct actatctttg ttatagctaa cttaaattct catttattaa gtatacctaa
     3481 gttttatgtt taaattttgt atagaatagt tgtatcactt aacaaagcta tgtttacgct
     3541 ttcaatcaag tctcatttat gatactatta tggtaataga aaaaattctt agttcgctaa
     3601 agacctttat tcgagatcca gaggaaatag aaattttatc cattaaaaat acgtttcata
     3661 agttacttac ttgatattaa tagaattcca atattgtata attaaatcaa agatgacact
     3721 ggaatcagga ggatttatta tggttaataa tgtagctgtt aaagtctcaa acctttcaaa
     3781 agaatttttg ttagggcaag acaaaactgt ctctattttg aaagatattt ccttatctgt
     3841 caattatggg gaatttgtat caatacttgg tgtcagtggt tctggaaagt ctactttgct
     3901 aagttgctta tcaagtttat ctgaaccaac aagtggcgaa gttgttatca atggtgtaaa
     3961 tccatatacc ttaaaagaag ggaagcttgc taaatttaga agacaagata ttgcaataat
     4021 ttttcaaaac tataatctgg tacctgctct acctgtacta gaaaatgtta cacttcctct
     4081 gagactatca ggaaagagtg ttgatagcaa taaagtaaaa aaaatgttgg atagtttgaa
     4141 ttttaaagca gaactatcat cattagttgc taccttatca ggtggagaac agcaaaaagt
     4201 ggctattact cgtgcaataa tagctgatag taaaattatt tttgctgatg agccaacagg
     4261 agctctggac agcgtctcaa gaaaacttat atttgaaaca cttcgtaatt tagcgagtca
     4321 aggaaaatgt gttcttatgg tcactcatga tattgagttg gcttcaaaaa ctgacagagc
     4381 actcatttta aaagacggaa aaatatctcg acaaattatt aaaccttcag ctgatgagct
     4441 ctaccaagca cttgaaagta gtaaagatta atttgaggag gatttattat gctaaaatta
     4501 atcatttacc aatttcagta ctcaaaaaga caatggttag gaacaatacc attactattt
     4561 gtatctagcc tgattgttga aacatcttta tttggtattg ctagttcaat caagactgct
     4621 aatattaacg cttcacaact ttttcaaatg ctaataattt ttggcgggac tactctattt
     4681 ttccttattt caaataatat aagactgcta atagatatct ttaaaaagga ttaccaatta
     4741 tggactatct taggcgcaag cagaactcag ctatctttat tagtatctgg acagttttat
     4801 ttaatggcag tgattgttag tagtattggt acaatacttt catttatcat ggcagatagt
     4861 tattacaaat ttttacaaaa tttattagga agagatgagt tacctgattt agttattaca
     4921 gccaacattc aatcaatttt attgagtatt tttatagtcc caacaattgt cgggataggg
     4981 gcttactttt attcaagtcg aatacttaaa atatcatcaa tattaaaacc aaaaaagaaa
     5041 aaaagaaaag ttacagtaac tggatttgtt aacatctctg ttcgtttatt cctatggcta
     5101 ctatgtatag gttctattgt ttcagcaggt tttatcagaa acaaagaaat aattgaaaaa
     5161 cagtcaagca taatattatt tttattaatt attcacatcc ttattattca atc
//
LOCUS       DAVKWZ010000093         5190 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000093 DAVKWZ010000000
VERSION     DAVKWZ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.093"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(336..1064)
                     /locus_tag="VW854_001998"
     CDS             complement(336..1064)
                     /locus_tag="VW854_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV0263176.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            1222..2631
                     /locus_tag="VW854_001999"
     CDS             1222..2631
                     /locus_tag="VW854_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV0263177.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFMKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLGLDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKAIYDIAMIVKEEYDNIPWFISENGMGVENEARFIGENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            2649..3944
                     /locus_tag="VW854_002000"
     CDS             2649..3944
                     /locus_tag="VW854_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV0263178.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            3949..4257
                     /locus_tag="VW854_002001"
     CDS             3949..4257
                     /locus_tag="VW854_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV0263179.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            4254..4562
                     /locus_tag="VW854_002002"
     CDS             4254..4562
                     /locus_tag="VW854_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV0263180.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         1743 a          876 c          947 g         1624 t
ORIGIN      
        1 caatttgtta gaagtcacag tctactattc taacctcagt atactatatg tgttacaaga
       61 aaagactcaa aaacttctcg tcagtgtaag aatgtttgaa acactattta aaactaaata
      121 actataagaa gtagatcagg tgaacaattt cagtacctac tctataagct atacgaagat
      181 atttttcaac atgatggata aaacgatcac tcttttaaag ccatcttatt tagaaaagag
      241 aaaccattta agcatagatg ggaccttctt gccctgtttt aaatttttct taaactagag
      301 cctgtaaaag attggcttca ttttcacatt ttctcctagg aggaatgtcg taatgcaaaa
      361 gaattaaatt caaataaatc atagcgatga cgactaatcg agtactcaaa cggagttccg
      421 ttatccaaat aagccacttg ttctacttca aataccggtt ccgttggaag gagctgcaag
      481 taatgttgct cattttctga actagaagaa gctcgcaaga tttttgtagc actggcaata
      541 tgcaatccta actttccttc aatgtaattg taaatcgatt tttgtaaaat atcttcagta
      601 atacctggaa taacatcggt actcatataa gtttgttcca gaacataagg tttaccgtca
      661 ataatacgca gacggtaaat attataaact ggactctgca aagcgacctg tagtttttct
      721 gctaaaaatt catttgcaaa ttctaatttg aagtgaataa tcctcgagtc aattgtagaa
      781 tgagcatctt cagatatttt tgtcagtccc cggatatctc tttctggaac gattaatttt
      841 ctttttgagc tgccacgaga gagaacaaag gttccctgcc cacgttttct aaaaattaag
      901 ccctcagaaa ctaacaagtc taaagctttt ttgatcgtca ttcgactaca ggcaaactgt
      961 ttagctaatg tcatttcatc aggcaattgt tcgttcatgg tatatttatt acaaagaatc
     1021 ttttcacgaa tctcattgta aataaataaa tattttgtac tcatttaatc tggttctctc
     1081 tataatattc taatagtagt ataacaaagt tcaagaaatg aggcaaacac aagatatgta
     1141 tcaacaattt ttaaaaatgt ctagacaaat tttaaaatta tgctatactt tatgtatagg
     1201 ataatagaaa ggaggttgag catgaaaaaa aagttagtat ttcctaatct gttttggtgg
     1261 ggagctgctt ctagcggacc tcagacagaa ggtcaatatg gaaaagtaca tgaaaatgtg
     1321 atggactact ggttcaaaac gcatccagaa gattttttcg ataatgtcgg acctcttgta
     1381 gccagtaact tttttcatac ttacaccgaa gatttccact tgatgaagga aattggagtt
     1441 aattctttcc gcacttccat ccaatggagt cgactcatca agaatttaga gacaggtgag
     1501 cctgatccaa aaggtattgc tttctacaat gccatcattg aagaagctaa aaagaaccag
     1561 atggatcttg tgatgaattt acatcatttt gatttaccag tggaacttct tcaaaaatac
     1621 ggtggttggg aaagcaaaca tgtagtggag ttattcatga agtttgccaa gactgctttc
     1681 acatgctttg gagataaggt tcattactgg acaactttca atgagccaat ggtcattcca
     1741 gaagcaggat acttatatgc tttccattat ccaaatctaa aaggaaaggg aaaagaggcc
     1801 gtacaagtca tctataatct aaaccttgct agtgcaaaag tgattcaact atatcgctca
     1861 ttaggacttg atggaaagat tgggattatt ttaaacttga cacctgctta tccaagaagt
     1921 aattctccag aagacttaga agcaagtcga tttacagatg acttctttaa caaagtcttc
     1981 ttgaatccag ctgttaaagg aactttccca gaaagattgg taaaacagct agagagagat
     2041 ggcgtgttat ggagtcatac cgaaaaagag cttcaactga tgaaatcaaa tacggttgat
     2101 tttcttggag taaactacta ccatccaaaa cgtgttcaag cacaagcaaa tcctgaggaa
     2161 tatcagacgc cctggatgcc agaccaatac ttcaaagagt atgaatggcc ggagcgtcgc
     2221 atgaatccat atcgtggttg ggaaattttt ccgaaagcca tttatgatat tgctatgatt
     2281 gtgaaggaag aatatgataa tattccatgg tttatcagtg aaaacggaat gggtgttgaa
     2341 aacgaagcac ggtttatcgg tgaaaatgga gttatcgatg acgtgtatcg tattgaattt
     2401 tatgaagaac atttaagatg gctacataaa gccattgaag agggaagtca ctgttttgga
     2461 taccacgctt ggaccgcatt tgattgctgg tcttggaata atgcatataa gaatcgttac
     2521 ggatttatct ccgttgattt agaaacgcaa aagagaacca tcaagagctc aggaagatgg
     2581 tatcgcaaag taagtgacaa taacggtttt gaagtagaaa ttgaggagta aagagaggag
     2641 atattcagat gaaaaactca aaatttatag atcaatttgc cacctttgct ggtaaactag
     2701 ggaaccaaat tcatttaaaa accctaagag atgcattcgt aacagtaatg ccattatata
     2761 ttttggcagg tttaatcgtt cttttgaaca acacggtatt taagtggatt ttccaagggg
     2821 atacattaac aagattccaa tattggggaa taacaattgc aaacggtact ttaagtattt
     2881 caggtatgat tattgctgta atggttggtt atttcttagc taaaaacaga gatttcgaaa
     2941 acccgttagc agcatcaatg ctatcattag tttctttaat tgtgatgatg ccaaatacag
     3001 tttctgtagt tcctgacgga gcaaaagatg ctgtaaacat ttcaggtgtt ctttcattca
     3061 acaacacagg tacaggcgca atgttcgccg gggttatcgt agcgattatt gcaacagaat
     3121 tattcattga attatcaaac gttaaagctt tacaaatgaa ccttggtgaa aatattccac
     3181 cagctgttag tagatcattt agcgtattac ttccagtcat gaccgtcatc tccttatttg
     3241 gggttgtttc agcattatta ttcaatataa ctggaatgaa cttaatctca atcattacaa
     3301 tctttattca agaaccaatt cgtcatattg gtacaagctt aatcggggtc attattattt
     3361 actctttagg aaatatgtta tggctatttg gtattcacca agcagttatt tacagtgcca
     3421 tcctagaacc attactatta attaacatta ctgaaaacat cactgcagca aataatggac
     3481 aagccattcc acacatcatc aacctatcac aaatacaaac attcgcttta atgggtggta
     3541 gtggatctac attatgttta ttaatagcaa cattcttagt aagtcgcaat gctgtttcta
     3601 aaaacgtggc taaattatct tttggacctg gtatcttcaa tatcaatgaa ccagtattat
     3661 tcggttaccc aatcgtttat aacatttcat tagctattcc atttatcaca gttccagtcc
     3721 ttggtatttt aatcagctac ttagcaacag ttacaggatt catgagtcct gcatttatac
     3781 aagttccttg gactacacca gtattcttaa atgcatggtt agcaacagca ggggacgtga
     3841 gagcagttct agttcaattc atcatctttg cacttggagt tcttctatac attccattta
     3901 tcaaagttaa tgacaaagtt gttgagcagg aaatggaagg ttaagattat gaaacaaatt
     3961 ttattagtat gtaatgcggg aatgtcgaca agtatgcttg ttaaaaaaat gcaacaaagt
     4021 gcgactgaac gtgggattga aatttctatc caagcaaaat caatgacaga agctaaaaag
     4081 aatatccatg aagcagatgt cattctaatt gggccacaaa ttcgttatga gttacttgct
     4141 gtgaaagaaa ttgcaggaaa tatccctgta gataccattg atatgcggga ttacggaatg
     4201 atgaatggag caaaagttct ggaacaagca cttgcatgga taggagaaat aagatgacag
     4261 aagaaatgga atatatctgt tttcagctca ttgccaatag tggagcagcc aaatcatcct
     4321 ttattgaagc catccaacta gctaaggcag gaaatttaaa agaagcgaaa attaaagttg
     4381 aagaagcgga agactcctta gtagaagcgc acaagattca ctctaatctc atacaaaaag
     4441 aagctactgg agaaaaaata ggattctcct tgctatttat gcatgcagaa gaccaaatgg
     4501 cttcaacgga gattattcag ttgctatcta aagaattcat cgatctctat caaaataaat
     4561 aggaacactg tcattcccat ctgtctttat taatgacggc tacattgtag gaacctccaa
     4621 aagttggaca aaatgattga aaggattgga tcctgcatta cacaagatcc aatccttttc
     4681 ttttactcta gtttttataa tatgttgaat ctagaatagt ccactgcagt ttctaaaaaa
     4741 tttctggaaa tggatgttaa gctccaattt atttgtttat atcttatttc agtttactat
     4801 actagcttat tattctagtg aaaaattgaa tgagccacta tttatttaat atgtatgatg
     4861 agggaatgac tcaaaaactt cttgtcgata taagaatatc tgagatagta ttttaaaacg
     4921 aaaggattat aaaagctcat ttccttcgca cttattttat agagatctat cattatactc
     4981 tttagaaatc aaaaagcaac acaccttgtc atcgttttat cgtaaatctg ttaatgaggg
     5041 ggctagtctt atctgcaatc tcaaatcagt gttttgagct gaatggcact cgttttctag
     5101 tttgctcttt gattttaatt gggccttagt aaatactact cttaaattaa actatagtga
     5161 attgaaataa gatgtgaaca actctatcag
//
LOCUS       DAVKWZ010000094         5151 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000094 DAVKWZ010000000
VERSION     DAVKWZ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.094"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            284..1829
                     /locus_tag="VW854_002003"
     rRNA            284..1829
                     /locus_tag="VW854_002003"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1881..1953
                     /locus_tag="VW854_002004"
     tRNA            1881..1953
                     /locus_tag="VW854_002004"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1914..1916,aa:Ala,seq:tgc)
     gene            2078..4979
                     /locus_tag="VW854_002005"
     rRNA            2078..4979
                     /locus_tag="VW854_002005"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            5056..>5151
                     /gene="rrf"
                     /locus_tag="VW854_002006"
     rRNA            5056..>5151
                     /gene="rrf"
                     /locus_tag="VW854_002006"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1460 a         1043 c         1507 g         1141 t
ORIGIN      
        1 ggtaacacgg gttcgaatcc cgtacggact atggtatgtt gcgtcaggac cacttgatga
       61 aaaaaagttt aaaaaaactt aaaaatcttc aaaaaagtgt tgacaagcga aagcagttgt
      121 gatatactaa tatagttgtc gcttgagaga agcaagtgac aaagaccttt gaaaactgaa
      181 caagacgaac caatgtgcag ggcgctacaa cataagttgt agtactgaac aatgaaaaaa
      241 acaataaatc tgtcagtgac agaaatgagt gagaactcaa acttttaatg agagtttgat
      301 cctggctcag gacgaacgct ggcggcgtgc ctaatacatg caagtagaac gctgaaggag
      361 gagcttgctt ctctggatga gttgcgaacg ggtgagtaac gcgtaggtaa cctgcctggt
      421 agcgggggat aactattgga aacgatagct aataccgcat aagagtagat gttgcatgac
      481 atttgcttaa aaggtgcact tgcatcacta ccagatggac ctgcgttgta ttagctagtt
      541 ggtggggtaa cggctcacca aggcgacgat acatagccga cctgagaggg tgatcggcca
      601 cactgggact gagacacggc ccagactcct acgggaggca gcagtaggga atcttcggca
      661 atggacggaa gtctgaccga gcaacgccgc gtgagtgaag aaggttttcg gatcgtaaag
      721 ctctgttgta agagaagaac gagtgtgaga gtggaaagtt cacactgtga cggtatctta
      781 ccagaaaggg acggctaact acgtgccagc agccgcggta atacgtaggt cccgagcgtt
      841 gtccggattt attgggcgta aagcgagcgc aggcggttag ataagtctga agttaaaggc
      901 tgtggcttaa ccatagtagg ctttggaaac tgtttaactt gagtgcaaga ggggagagtg
      961 gaattccatg tgtagcggtg aaatgcgtag atatatggag gaacaccggt ggcgaaagcg
     1021 gctctctggc ttgtaactga cgctgaggct cgaaagcgtg gggagcaaac aggattagat
     1081 accctggtag tccacgctgt aaacgatgag tgctaggtgt tagacccttt ccggggttta
     1141 gtgccgtagc taacgcatta agcactccgc ctggggagta cgaccgcaag gttgaaactc
     1201 aaaggaattg acgggggccc gcacaagcgg tggagcatgt ggtttaattc gaagcaacgc
     1261 gaagaacctt accaggtctt gacatccctc tgaccgctct agagatagag ttttccttcg
     1321 ggacagaggt gacaggtggt gcatggttgt cgtcagctcg tgtcgtgaga tgttgggtta
     1381 agtcccgcaa cgagcgcaac ccctattgtt agttgccatc atttagttgg gcactctagc
     1441 gagactgccg gtaataaacc ggaggaaggt ggggatgacg tcaaatcatc atgcccctta
     1501 tgacctgggc tacacacgtg ctacaatggc tggtacaacg agtcgcaagc cggtgacggc
     1561 aagctaatct cttaaagcca gtctcagttc ggattgtagg ctgcaactcg cctacatgaa
     1621 gtcggaatcg ctagtaatcg cggatcagca cgccgcggtg aatacgttcc cgggccttgt
     1681 acacaccgcc cgtcacacca cgagagtttg taacacccga agtcggtgag gtaaccgtaa
     1741 ggagccagcc gcctaaggtg ggatagatga ttggggtgaa gtcgtaacaa ggtagccgta
     1801 tcggaaggtg cggctggatc acctcctttc taaggataag gaactgcgca ttggtcttgt
     1861 ttagtcttga gaggtcttgt ggggccttag ctcagctggg agagcgcctg ctttgcacgc
     1921 aggaggtcag cggttcgatc ccgctaggct ccattggtga gagatcacca agtaatgcac
     1981 attgaaaatt gaatatctat atcaaatagt aacaagaaaa taaaccgaaa acgctgtagt
     2041 attaataaga gtttatgact gaaaggtcag aaaataaggt taagttaata agggcgcacg
     2101 gtggatgcct tggcactagg agccgacgaa ggacgtgaca aacgacgata tgccttgggt
     2161 agctgtaagt aagcgatgat ccagggattt ccgaatgggg gaacccaaca ggtagtacct
     2221 gttacccaca tctgttaagg atgtgaggag gaagacgcag tgaactgaaa catctaagta
     2281 gctgcaggaa gagaaagcaa aagcgattgc cttagtagcg gcgagcgaaa cggcaggagg
     2341 gcaaaccgaa gagtttactc ttcggggttg taggactgca atgtggactc aaagattata
     2401 gaagaatgat ttgggaagat cagccaaaga gagtaatagc ctcgtattta aaatagtctt
     2461 tgtacttagc agtatcctga gtacggcggg acacgtgaaa tcccgtcgga atctgggagg
     2521 accatctccc aaccctaaat actccctagt gaccgatagt gaaccagtac cgtgagggaa
     2581 aggtgaaaag caccccggga ggggagtgaa atagaacctg aaaccgtgtg cctacaacaa
     2641 gttcgagccc gttaatgggt gagagcgtgc cttttgtaga atgaaccggc gagttacgtt
     2701 atgatgcgag gttaagttga agagacggag ccgtagggaa accgagtctg aatagggcgc
     2761 cttagtatca tgacgtagac ccgaaaccat gtgacctacc catgagcagg ttgaaggtgc
     2821 ggtaagacgc actggaggac cgaaccaggg cacgttgaaa agtgcttgga tgacttgtgg
     2881 gtagcggaga aattccaaac gaacttggag atagctggtt ctctccgaaa tagctttagg
     2941 gctagcgtcg acattagaga ttcttggagg tagagcactg tttgggtgag gggtccatcc
     3001 cggattacca atctcagata aactccgaat gccaatgaat tatggtcggc agtcagactg
     3061 cgagtgctaa gatccgtagt cgaaagggaa acagcccaga ccaccagcta aggtcccaaa
     3121 ataattgtta agtggaaaag gatgtggggt tgcacagaca actaggatgt tagcttagaa
     3181 gcagctattc attcaaagag tgcgtaatag ctcactagtc gagtgaccct gcgccgaaaa
     3241 tgtaccgggg ctaaaacaat ttaccgaagc tgtggatacc tttataggta tggtaggaga
     3301 gcgttctatg tgtgatgaag gtataccgtg aggagtgctg gaacgcatag aagtgagaat
     3361 gccggtatga gtagcgaaag acaggtgaga atcctgtcca ccgtaagact aaggtttcca
     3421 ggggaaggct cgtccgccct gggttagtcg ggacctaagg agagaccgaa aggtgtatcc
     3481 gatggacaac aggttgatat tcctgtacta gagtatgtag tgatggaggg acgcagtagg
     3541 ctaactaaag cagacgattg gaagagtctg tctaagcagt gaggtgtgaa ttgagtcaaa
     3601 tgcttaattc tataacattg agctgtgatg gggagcgaag tttagtagcg aagttagtga
     3661 cgtcacactg ccaagaaaag cttctagcgt ttaaacatac tctacccgta ccgcaaaccg
     3721 acacaggtag tcgaggcgag tagcctcagg tgagcgagag aactctcgtt aaggaactcg
     3781 gcaaaatgac cccgtaactt cgggagaagg ggtgctgact taaagtcagc cgcagtgaat
     3841 aggcccaagc aactgtttat caaaaacaca gctctctgct aaatcgtaag atgatgtata
     3901 gggggtgacg cctgcccggt gctggaaggt taagaggagt gcttagcgta agcgaaggta
     3961 tgaattgaag ccccagtaaa cggcggccgt aactataacg gtcctaaggt agcgaaattc
     4021 cttgtcgggt aagttccgac ccgcacgaaa ggcgtaatga tttgggcact gtctcaacga
     4081 gagactcggt gaaattttag tacctgtgaa gatgcaggtt acccgcgaca ggacggaaag
     4141 accccatgga gctttactgc agtttgatat tgagtgtctg taccacatgt acaggatagg
     4201 taggagtcta agagatcggg acgccagttt cgaaggagac gctgttggga tactaccctt
     4261 gtgttatggc cactctaacc cagataggtg atccctatcg gagacagtgt ctgacgggca
     4321 gtttgactgg ggcggtcgcc tcctaaaagg taacggaggc gcccaaaggt tccctcagaa
     4381 tggttggaaa tcattcgcag agtgtaaagg tataagggag cttgactgcg agagctacaa
     4441 ctcgagcagg gacgaaagtc gggcttagtg atccggtggt tccgtatgga agggccatcg
     4501 ctcaacggat aaaagctacc ctggggataa caggcttatc tcccccaaga gttcacatcg
     4561 acggggaggt ttggcacctc gatgtcggct cgtcgcatcc tggggctgta gtcggtccca
     4621 agggttgggc tgttcgccca ttaaagcggc acgcgagctg ggttcagaac gtcgtgagac
     4681 agttcggtcc ctatccgtcg cgggcgtagg aaatttgaga ggatctgctc ctagtacgag
     4741 aggaccagag tggacttacc gctggtgtac cagttgtctt gccaaaggca tcgctgggta
     4801 gctatgtagg gaagggataa acgctgaaag catctaagtg tgaaacccac ctcaagatga
     4861 gatttcccat gattatatat cagtaagagc cctgagagat gatcaggtag ataggttaga
     4921 agtggaagtg tggcgacaca tgtagcggac taatactaat agctcgagga cttatccaaa
     4981 gtaactgaga atatgaaagc gaacggtttt cttaagttga atagatattc aattttgagt
     5041 aggtattact cagagttaag tgacgatagc ctaggagata cacctgtacc catgccgaac
     5101 acagaagtta agccctagaa cgccggaagt agttgggggt tgccccctgt g
//
LOCUS       DAVKWZ010000095         4983 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000095 DAVKWZ010000000
VERSION     DAVKWZ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.095"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            20..466
                     /gene="argR"
                     /locus_tag="VW854_002007"
     CDS             20..466
                     /gene="argR"
                     /locus_tag="VW854_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV0263181.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            517..3051
                     /gene="mutS"
                     /locus_tag="VW854_002008"
     CDS             517..3051
                     /gene="mutS"
                     /locus_tag="VW854_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEV0263182.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVAIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            3198..4892
                     /gene="patA"
                     /locus_tag="VW854_002009"
     CDS             3198..4892
                     /gene="patA"
                     /locus_tag="VW854_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEV0263183.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLDALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELFGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQNGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
BASE COUNT         1366 a          968 c         1223 g         1426 t
ORIGIN      
        1 atgcacgcaa gaggatttta tgagaaaaag agatcgtcat cagttaataa aaaaaatgat
       61 tactgaggag aaattaagta cacaaaaaga aattcaagat cggttggagg cgcacaatgt
      121 ttgtgtgacg cagacaacct tgtctcgtga tttgcgcgaa atcggcttga ccaaggtcaa
      181 gaaaaatgat atggtgtatt atgtactagt aaatgagaca gaaaagattg atttggtgga
      241 atttttgtct catcatttag aaggtgttgc aagagcagag tttaccttgg tgcttcatac
      301 caaattggga gaagcctctg ttttggcaaa tattgtagat gtaaacaagg atgaatggat
      361 tttaggaaca gttgctggtg ccaatacctt attggttatt tgtcgagatc agcacgttgc
      421 caaactcatg gaagatcgtt tgctagattt gatgaaagat aagtaaggtc ttgggagttg
      481 ctctcaagac ttatttttga aaaggagaga cagaaaatgg cgatagaaaa gctatcaccc
      541 ggcatgcaac agtatgtgga tattaaaaag caatatccag atgctttttt gctctttcgg
      601 atgggtgatt tttatgaatt attttatgag gatgcggtca atgctgcgca gattctggaa
      661 atttccttaa cgagtcgcaa caagaatgcc gacaatccga tccctatggc gggtgttccc
      721 tatcattctg ctcaacagta tatcgatgtc ttgattgagc agggttataa ggtggctatc
      781 gcagagcaga tggaagatcc taaacaagca gttggggttg ttaaacgaga ggttgttcag
      841 gtcattacgc cagggacagt ggtcgatagc agtaagccgg acagtcagaa taattttttg
      901 gttgccatag accgcgaagg caatcaattt ggcctagctt atatggattt ggtgacgggt
      961 gacttttatg tgacaggtct tttggatttc acgctggttt gtggggaaat ccgtaacctc
     1021 aaggctcgag aagtggtgtt gggttatgac ttgtctgagg aagaagaaca aatcctcagc
     1081 cgccagatga atctggtact ctcttatgaa aaagaaagct ttgaagacct tcatttattg
     1141 gatttgcgat tggcaacggt ggagcaaacg gcatctagta agctgctcca gtatgttcat
     1201 cggactcaga tgagggaatt gaaccacctc aaacctgtta tccgctacga aattaaggat
     1261 ttcttgcaga tggattatgc gaccaaggct agtctggatt tggttgagaa tgctcgctca
     1321 ggtaagaaac aaggcagtct tttctggctt ttggatgaaa ccaaaacggc tatggggatg
     1381 cgtctcttgc gttcttggat tcatcgcccc ttgattgata aggaacgaat cgtccaacgt
     1441 caagaagtag tgcaggtctt tctcgaccat ttctttgagc gtagtgactt gacagacagt
     1501 ctcaagggtg tttatgacat tgagcgcttg gctagtcgtg tttcttttgg caaaaccaat
     1561 ccaaaggatc tcttgcagtt ggcgactacc ttgtctagtg tgccacggat tcgtgcgatt
     1621 ttagaaggga tggagcaacc tactctagcc tatctcatcg cacaactgga tgcaatccct
     1681 gagttggaga gtttgattag cgcagcgatt gctcctgaag ctcctcatgt gattacagat
     1741 gggggaatta tccggactgg atttgatgag actttagaca agtatcgttg cgttctcaga
     1801 gaagggacta gctggattgc tgagattgag gctaaggagc gagaaaactc tggtatcagc
     1861 acgctcaaga ttgactacaa taaaaaggat ggctactatt ttcatgtgac caattcgcaa
     1921 ctaggaaatg tgccagccca ctttttccgc aaggcgacgc tgaaaaactc agaacgcttt
     1981 ggaaccgaag aattagcccg tatcgaggga gatatgcttg aggcgcgtga gaagtcagcc
     2041 aacctcgaat acgaaatatt tatgcgcatt cgtgaagagg tcggcaagta catccagcgt
     2101 ttacaagctc tagcccaagg aattgcgacg gttgatgtct tacagagtct ggcggttgtg
     2161 gctgaaaccc agcatttgat tcgacctgag tttggtgacg attcacaaat tgatatccgg
     2221 aaagggcgcc atgctgtcgt tgaaaaggtt atgggggctc agacctatat tccaaatacg
     2281 attcagatgg cagaagatac cagtattcaa ctggttacag ggccaaacat gagtgggaag
     2341 tctacctata tgcgtcagtt agccatgacg gcggttatgg cccagctggg ttcctatgtt
     2401 cctgctgaaa gcgcccattt accgattttt gatgcgattt ttacccgtat cggagcagca
     2461 gatgacttgg tttcgggtca gtcaaccttt atggtggaga tgatggaggc caataatgcc
     2521 atttcgcatg cgaccaagaa ctctctcatt ctctttgatg aattgggacg tggaactgca
     2581 acttatgacg ggatggctct tgctcagtcc atcatcgaat atatccatga gcacatcgga
     2641 gctaagaccc tctttgcgac ccactaccat gagttgacta gtctggagtc tagtttacaa
     2701 cacttggtca atgtccacgt ggcaactttg gagcaggatg ggcaggtcac cttccttcac
     2761 aagattgaac cgggaccagc tgataaatcc tacggtatcc atgttgccaa gattgctggc
     2821 ttgccagcag accttttagc aagggcggat aagattttga ctcagctaga gaatcaagga
     2881 acagagagtc ctcctcccat gagacaaact agtgctgtca ctgaacagat ttcactcttt
     2941 gatagggcag aagagcatcc tatcctagca gaattagcta aactggatgt gtataatatg
     3001 acacctatgc aggttatgaa tgtcttagta gagttaaaac agaaactata aaaccaagac
     3061 tcactagtta atctagctgt atcaaggaga cttctttgac aattctccac ttttttgcta
     3121 gaataacatc acacaaacag aatgaaaagg agctgacgca ttgtcgctcc cttttgtcta
     3181 ttttttaagg agaaagtatg ctgattcaga aaataaaaac ctacaagtgg caggccttgg
     3241 cttcgctcct gatgacaggc ttgatggttg ctagttcact tctgcaaccg cgttatctgc
     3301 aggaagtatt agacgccctc cttactggga aatatgaagc tatttatagt atcggggctt
     3361 ggttgattgg tgtggccgta gtcggtctag ttgctggtgg actcaatgtt gtcctcgcag
     3421 cctatattgc ccaaggagtt tcatccgacc ttcgggagga tgccttccgt aaaattcaaa
     3481 ccttttctta tgctgatatt gaacaattta atgcgggaaa tctagtcgtt cgaatgacaa
     3541 atgatatcaa ccagattcag aacgttgtca tgatgacctt ccaaattctt ttcagacttc
     3601 ccctcttgtt catcggttcg tttatcctag cggttcaaac cttaccttct ctgtggtggg
     3661 tgattgttct catggtagtc ttgatttttg gtttgactgc tgtcatgatg ggaatgatgg
     3721 ggcctcgttt tgccaagttt caaacccttc ttgagcgcat caatgccatt gccaaggaaa
     3781 atttacgtgg cgttcgtgtg gtcaagtcct ttgtccaaga aaaagagcaa tttgctaagt
     3841 ttacagaggt ctcagacgag ctttttggtc aaaaccttta cattggttat gccttttcag
     3901 tagtggaacc ctttatgatg ttggttggtt acggggcggt cttcctctct atttggctgg
     3961 tcgcgggaat ggttcagtcg gatccgtctg ttgttggttc catcgcttct tttgttaatt
     4021 acctaagcca gattatcttt accattgtta tggttggatt tttgggaaat tctgtcagcc
     4081 gtgccatgat ttccatgcgt cgtattcgag aaattcttga cgcagagcca gctatgacct
     4141 tcaaggatat cccagatgaa gagttggttg gaagccttag ctttgaaaat gtgaccttta
     4201 cctatccaat ggacaaggaa ccgatgctga aagatgtgag ctttactatt gaacctggtc
     4261 aaatggttgg tgtagttgga gcgactggtg caggaaagtc aaccttggct caattgattc
     4321 cacgtctctt tgatccacag aacggggcca ttaaaatcgg tggcaaggat attcgagaag
     4381 tgagtgaagg aaccctgcgt aaaacagttt ccatcgttct ccaacgtgcc attcttttta
     4441 gtggaacgat tgcagataac ttgagacagg ggaagggaaa tgctactcta tttgaaatgg
     4501 agcgcgcagc caatattgcc caggctagtg aattcattca tcgtatggag aaaacctttg
     4561 aaagtccagt tgaagaacgg ggaaccaatt tctctggtgg acaaaaacaa aggatgtcga
     4621 ttgcgcgtgg gattgtcagc aatccacgta ttctgatttt tgatgattcg acctcagcct
     4681 tggatgccaa atcagagcgc ttggtgcaag aagctttgaa taaggacttg aaggggacga
     4741 caaccattat tattgctcaa aaaattagct cggttgtcca tgcagacaag atcttggttc
     4801 taaatcaagg acgattgatt ggtcaaggta cgcatgcaga cttggttgcc aacaatgccg
     4861 tttaccgtga aatctatgaa acacagaaat gaaagacaaa ctataagaaa agtcaatagt
     4921 tttatctaaa ctatttctta tttcaatttg atgatttggc gatgatttta gagcacggca
     4981 aaa
//
LOCUS       DAVKWZ010000096         4368 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000096 DAVKWZ010000000
VERSION     DAVKWZ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.096"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            7..1032
                     /locus_tag="VW854_002010"
     CDS             7..1032
                     /locus_tag="VW854_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV0263184.1"
                     /translation="MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDH
                     AFGQTILDKKPERVATIAWGNHDVALALGIVPVGLSKANYGVSADKGVLPWTEEKIKE
                     LNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQT
                     LWRDMIRIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAAD
                     TSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITY
                     GDDKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLG
                     NACKNAK"
     gene            1022..2029
                     /locus_tag="VW854_002011"
     CDS             1022..2029
                     /locus_tag="VW854_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV0263185.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLAAFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVN
                     N"
     gene            2029..3036
                     /locus_tag="VW854_002012"
     CDS             2029..3036
                     /locus_tag="VW854_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV0263186.1"
                     /translation="MQNLIIGIQKRKNRITLFSSLFLLIIISLSFFILLIGDESYSFS
                     TLIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQTMLRNPLASPDIIG
                     VTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSSLILFLLAKKDGFSAARLIILGIG
                     FQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLIVSIIATILVLFFN
                     KRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPIACISFLAGPIALN
                     IGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSPYLIYLLIKMNRRN
                     I"
     gene            3036..3830
                     /locus_tag="VW854_002013"
     CDS             3036..3830
                     /locus_tag="VW854_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0263187.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVMANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHVNS"
BASE COUNT         1577 a          637 c          742 g         1412 t
ORIGIN      
        1 gtttttatga aaaacaaatt ttttctaata gctattttag ctatgtgtat agtttttagc
       61 gcttgttctt ctaattctgt taaaaatgaa gaaaatactt ctaaagagca tgcgcctgat
      121 aaaatagttt tagatcatgc tttcggtcaa actatattag ataaaaaacc tgaaagagtt
      181 gcaactattg cttggggaaa tcatgatgta gcattagctt taggaatagt tcctgttgga
      241 ctttcaaaag caaattacgg tgtaagtgct gataaaggag ttttaccatg gacagaagaa
      301 aaaatcaaag aactaaatgg taaagctaac ctatttgacg atttggatgg acttaacttt
      361 gaagcaatat caaattctaa accagatgtt atcttagcag gttattctgg tataactaaa
      421 gaagattatg acactctatc aaaaattgct cctgtagcag catacaaatc taaaccttgg
      481 caaactttat ggagagatat gattagaatt gattcaaaag ccttaggtat ggaaaaagaa
      541 ggtgatgagt taatcaaaaa tactgaagct cgtatatcca aagaattaga aaaacatcca
      601 gaaatcaaag gaaaaatcaa aggaaaaaaa gtattattta ctatgattaa tgctgcagat
      661 acatcaaaat tctggattta tactagcaaa gatccaagag caaattattt aacagattta
      721 ggtctagttt tccctgaatc attaaaagaa tttgagagtg aagatagttt tgcaaaggaa
      781 atttctgcag aagaagcaaa taagataaat gatgctgatg taatcataac ttatggtgat
      841 gataaaactc ttgaagcttt acaaaaagat cctcttttag gtaaaataaa tgcaattaaa
      901 aatggtgccg ttgctgtaat tccagataat acaccgttag cagcctcatg cactccaaca
      961 ccactttcaa taaactatac tattgaagaa tatctaaatc ttttaggaaa tgcatgcaaa
     1021 aatgcgaaat aaaaaacaaa taaacctagg cataattttt gtaatctgcc taggtcttct
     1081 tattacaata tttttgtcat taaagcttgg aacaaaagaa attaatatca gagatttttt
     1141 agcagctttt ggaatgggta atacaaatga tgattttatt aaatcaatta tatataaaag
     1201 aatacctaga actatttttg caattttagc aggttctagt cttgccataa gcggtgtatt
     1261 gatgcaatca gttactagaa acccaatagc tgatccaggt atactcggta taaacacagg
     1321 agcaagtctt agtgtagtaa ttggtctttc ttttttagga atttcatcaa gcataagcca
     1381 tataagtttt gcaatcattg gtggcttagt aagtgcaatt tttgtatacg cgattgctgt
     1441 aagcggaaaa gcaggcctta cccctataaa acttgcctta tcaggaactt gtgttagtat
     1501 ggctttaagc agttttgtaa gttttttaat tttaccgaat aataacgtct tagacaaatt
     1561 tagattttgg caaataggta gccttggagc agctacatta tcttctatat ctacactact
     1621 accttttata attttaggtc acttgatagc tatatttatt tcatcagatt taaacgcttt
     1681 agctatgggt gatgaaatgg ctgttggtct tggagttaat gttaatagga taagatcact
     1741 tgcaataatt gcaagtgtgc ttttatgttc aagtattact gcaattggtg gacctattgg
     1801 cttcgtaggt cttatagttc ctcacttttg tggcttattt ataagcaaag atatacgcac
     1861 aatgaccatt tcttcatctt ttataggtgc agagctcttg cttatatgtg atataatcgg
     1921 ccgtatgtta ggtaaaccag gtgaaattga agtagggata attactgcaa taatcggggg
     1981 tccagtactt atttatgtaa ctatgaaaaa tagaggggtt aataactaat gcaaaattta
     2041 attataggta ttcaaaaaag aaaaaataga ataacactat tctcctcact atttttatta
     2101 ataataatca gtctatcatt tttcatttta cttatcggag atgaaagtta ttctttttca
     2161 actttgatta aagtcttaaa tagtgaaact gttcctggag ctagtttttc gattatggaa
     2221 attagattac caaaattatt agcaggaatt atagctggct ggtcttttgg attggcagga
     2281 tttatctttc aaactatgtt aagaaatcct cttgcaagtc ctgatataat cggtgtcaca
     2341 agttcttcat ctattgcagc ggtcttttgc atattggtat taaaaacaaa tagtttaact
     2401 actggaatta tttcaataac ttgtggacta acatcatctt taatattatt tttactagct
     2461 aaaaaagatg gtttttcagc agcaagactg ataatattag gtattggttt tcaagctgtc
     2521 acaagagcag gcacctcatt tttattgttg aaagtagcaa gatatgaatt acaagaagtt
     2581 atgagatggc tcagtggctc tttatctttt acaaagttag atgacatacc tcttgttcta
     2641 atagtaagta ttattgctac tatattagtt ttatttttta ataaaagact agaaattatt
     2701 gaacttggtg aagaaatagc aatcggactt ggtgcaaatc ccgagctttc aaggcttgtt
     2761 ttaatttttt gcgctgtatc tttaactgct ttttctactt caattacagg accaatagct
     2821 tgtatatctt ttttagctgg tcccatagcc ttaaatattg gcaagaaaag aagcccaata
     2881 ttagctggat tggttggaat tttactagtt ttgttatcag acatattctc tcaaaatatt
     2941 ttaccagcta gatatccagt aggtgttgta actggcttgt taggttcacc atacttaata
     3001 tatttactaa taaaaatgaa caggaggaat atataatgaa aggtctgtgg tcaaataatc
     3061 ttacctgcgg ttatgatgaa aaaataatct tggaaaatat aaatataaaa atacctgaag
     3121 aaaaaatatc agttattatt gggtcaaatg gttgtgggaa atcaacactc attaaaacct
     3181 tgtctcgact tataaagcca ttagagggag aagtattgct tgataataaa tcaattaatt
     3241 cttataaaga aaaagattta gcaaaacaca tagctatatt acctcaatct ccaataatcc
     3301 ctgaatcaat aacagtagct gatcttgtaa gccgtggtcg tttcccctac agaaagcctt
     3361 ttaagagtct tggaaaagat gaccttgaaa taataaacag atcaatggtt atggccaatg
     3421 ttgaagatct agcaaataac ctagttgaag aactttctgg gggtcaaagg caaagagtat
     3481 ggatagctct agccctagcc caagatacaa gtatcctact tttagatgag ccaactactt
     3541 acttggatat ctcatatcaa atagaactat tagacctctt gactgatcta aaccaaaaat
     3601 ataagacaac catttgcatg attttgcacg atataaatct aacagcaaga tacgctgatt
     3661 acctatttgc aattaaagaa ggtaaacttg ttgcagaggg aaagcctgaa gatatactaa
     3721 atgataaact agttaaagat atctttaatc ttgaagcaaa aattatacgt gaccctattt
     3781 ccaattcgcc tctaatgatt cctattggca agcaccatgt taactcttaa taattgaata
     3841 ttggaattca tttaaaagtc gcaacttttg aactgaccct caaaagttag gacaaaaaac
     3901 taactaaagg aggtcagttt tttaatggca aatacagcac agaatttaag ataaaattag
     3961 taaaagaata tcatgaatga aaaataggat atagagaata acagcaatat taaaaagatc
     4021 aggactgata ataaacaaaa agaaagtaca acgactagtt caaaagctaa aacttcaagt
     4081 aaaaagttat tcaagaaaat ctagaaaata ctcatcctac aaaggacagg taggaaaaat
     4141 atcagacaac aaaataaaaa gaaatattta ttaccgaaaa aaaggggaaa tcgagtcaag
     4201 taccaacagg ctcatctatt ccaaaaagat atttactcga tggaaaaact cctagaatta
     4261 gtgctacaaa tattgacaat ggtatcttag gttactacga agatatagat gataaaaatt
     4321 atagagtttt tgaaaatttt atatctgttt catttttagg agcagtct
//
LOCUS       DAVKWZ010000097         4259 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000097 DAVKWZ010000000
VERSION     DAVKWZ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.097"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            354..2855
                     /locus_tag="VW854_002014"
     CDS             354..2855
                     /locus_tag="VW854_002014"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEV0263188.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEIPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVAEAAYTEDGLHVNSDFLDGLNKEEA
                     IAKIVAWLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTRADGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KVYNETVKSVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFVQLIAPFAPHL
                     AEELWQTVAATGESISYVTWPTWDESKLVEDEIEIVVQIKGKVRAKIMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVIVVPNKLVNIVVK"
     gene            3049..3744
                     /locus_tag="VW854_002015"
     CDS             3049..3744
                     /locus_tag="VW854_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0263189.1"
                     /translation="MPVNEYGQMIGESMEAYTPGELPSFDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPREMWTYLFQEPVANLEELVTLLNQMLARKDRFYYAIIDKATGKALG
                     TLSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYVFDELNYRRYEWKCDAL
                     NLPSRRAAERLGFVYEGTFRQAVVYKGRTRDTDWLSMIDKDWPKVKSRLEIWLRPENF
                     DKNGQQYKSLREF"
     gene            3756..4172
                     /locus_tag="VW854_002016"
     CDS             3756..4172
                     /locus_tag="VW854_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0263190.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPQMLEQALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVFVQDLIVLPSYQRQGIGSSLMKQALEDFKEAYQVQ
                     LATEQTEKNVGFYRSMGFETLSTYDCTGMIWVNKEK"
BASE COUNT         1260 a          813 c          984 g         1202 t
ORIGIN      
        1 ctataaatgt tttagcaatt gtttcgtact atttcagatt cagtctacta tacgttcttc
       61 ataaatcaaa aagcgacata ggtagtcggc tatttattgt gaatacatta attagcattc
      121 cagttttatc ttcggtctaa aataagtatt ttgtgctata cgagataagc ttcttgactt
      181 actccttgat ttattccttg atttactgca taacaatggg ataaaaagtg ggagatagag
      241 caattcatag tcatcaaaat taatgagata cagtatacag tttttccttt aaacacattt
      301 caaattccct caaaaatggt ataatagtaa catcacaaaa ttggagagag accatgagtt
      361 tttacaatca taaagaaatt gagcctaagt ggcagggcta ctgggcagaa catcatacat
      421 ttaagacagg aacagatgca tcaaaaccta agttttatgc gcttgatatg ttcccttatc
      481 cgtctggagc tggtctgcac gtaggacacc cagaaggtta tactgcaacc gatatcctca
      541 gtcgttacaa acgtgcgcaa ggctacaatg tccttcaccc aatgggttgg gatgcttttg
      601 gtttgcctgc agagcaatac gctatggata ctggtaatga cccagcagaa tttacagcgg
      661 aaaacattgc caacttcaaa cgtcaaatca atgcgcttgg attttcttat gactgggatc
      721 gtgaagtcaa cacaacagat ccaaactact acaagtggac tcaatggatt ttcaccaagc
      781 tttacgaaaa aggcttggcc tatgaagctg aagtgccagt aaactgggtt gaggaattgg
      841 gaactgccat tgccaatgaa gaagtgcttc ctgacggaac ttctgagcgt ggaggctatc
      901 cagttgtccg caaaccaatg cgccaatgga tgctcaaaat cacggcttac gcagagcgct
      961 tgctcaatga cttagatgaa ctagattggt cagagtctat caaggatatg caacgcaact
     1021 ggattggtaa atcaactggt gccaatgtaa ctttcaaagt aaaaggaaca gacaaggaat
     1081 ttacagtctt tactactcgt ccggacacac ttttcggtgc gactttcact gtcttggctc
     1141 ctgaacatga attagtagac gctatcacaa gttcagagca agcagaagct gtagcagact
     1201 ataaacacca agctagcctc aaatctgact tggctcgtac agaccttgcc aaagaaaaaa
     1261 ctggtgtttg gactggtgct tatgccatca accctgtcaa tggtaaggaa attccaatct
     1321 ggattgccga ctatgttctt gctagttacg gaacaggtgc cgttatggcc gttccagctc
     1381 acgaccaacg tgactgggaa tttgccaaac aatttgacct tccaatcgtc gaagtgcttg
     1441 aaggtggaaa tgtagcagaa gctgcctata cagaggatgg tcttcatgtc aattcagact
     1501 tcctagatgg actcaacaaa gaagaagcta ttgccaagat tgtggcttgg ttggaagaaa
     1561 aaggctgtgg acaagagaag gttacctacc gtctccgcga ctggctcttt agccgtcaac
     1621 gttactgggg tgaaccaatt cctatcattc attgggaaga tgggacttca acagctgttc
     1681 ctgaaagtga attgccactt gtcttgcctg taaccaagga tatccgccct tcaggtacag
     1741 gggaaagtcc attggctaac ttgacagatt ggcttgaagt gactcgtgca gatggtgtca
     1801 aaggtcgtcg tgaaaccaac actatgccac aatgggcagg ttcaagctgg tactacctcc
     1861 gctatattga cccacacaat actgagaaat tggctgatga ggatcttctc aaacaatggt
     1921 tgccagtaga tatctacgtg ggtggtgcag agcatgctgt tcttcacttg ctctacgctc
     1981 gtttctggca taaattcctc tatgacctcg gtgttgttcc aactaaggaa ccattccaaa
     2041 aactctttaa ccaaggaatg attttgggaa caagctaccg tgaccaccgt ggggctcttg
     2101 tggcaaccga taaggttgaa aaacgtgacg gttctttctt ccatgtggaa acaggagagg
     2161 agttggagca agcgccagcc aagatgtcta aatctcttaa gaatgttgtc aacccagacg
     2221 atgtggtgga gcaatacggt gccgataccc ttcgtgtcta tgaaatgttt atgggaccac
     2281 ttgatgcttc gattgcttgg tcagaagaag gtctggaagg aagccgtaaa ttccttgacc
     2341 gtgtttaccg tttgattaca agtaaagaaa tccttgcgga aaacaatggc gctcttgata
     2401 aggtttacaa tgaaacagtc aaatctgtca cagagcaaat tgagtccctc aaattcaaca
     2461 ccgccattgc ccaacttatg gtctttgtca atgcggctaa caaggaagac aagctctatg
     2521 ttgactacgc caaaggcttc gttcagttga ttgcaccatt tgcacctcac ttggcagaag
     2581 agctctggca aacagttgcg gcaacaggtg agtcaatctc ttacgtgact tggccaacat
     2641 gggatgaaag caaattggtt gaagacgaaa tcgaaatcgt cgtccaaatc aaaggtaaag
     2701 ttcgtgccaa aatcatggtt gctaaagacc tatcacgcga agaattgcaa gagattgctc
     2761 tagctgatga aaaagtcaaa gcagaaattg atggtaagga aatcgtgaaa gtgattgtgg
     2821 taccgaataa attggttaat attgttgtga aataaccaat ttatttgctg gtttcaaatc
     2881 tcggacagtt tcctaaactg tttgagccag ttaaactcgt taatattgtt ttgaaataag
     2941 ataggagtcc ttcagagtag aatctggggg attttttgaa tcttcttatg aaagtatgat
     3001 atactatggg caactataaa gtttgaaaag tgaaataagg agaataagat gccagtaaat
     3061 gaatatggtc aaatgattgg ggagtcaatg gaagcttata ctccaggtga attgccttct
     3121 tttgatttct tagaagggcg ttatgctcga atagaagctc tttcagtgga aaagcacgcg
     3181 gaggatttat tagctgttta tggccctgat actcctcggg agatgtggac ctacctcttt
     3241 caggagccag tggcaaatct ggaggagctg gttactcttt taaatcagat gttggctcgt
     3301 aaggaccgtt tttactatgc aattatagac aaggcaactg gtaaggcttt gggaactttg
     3361 tctctcatgc gtattgacca gaataaccga gtaatagaag tgggagctgt cactttttct
     3421 ccagagctca gggggacacg gataggaaca gaagctcagt atcttttggc tcgctatgtc
     3481 tttgatgagc ttaactatcg tcgctatgag tggaaatgcg atgctcttaa cctgccatcc
     3541 agacgagctg cggagcgttt gggatttgtc tacgaaggaa cctttcgtca ggcagtggtt
     3601 tataaggggc gtacgaggga tacggattgg ttgtctatga ttgataagga ctggcctaaa
     3661 gtcaaatctc gtttggaaat atggttgcgt cctgaaaact ttgataaaaa tggacagcag
     3721 tacaagagct tgagagaatt ctaagaggtg ttgagataat tactattaaa aagcaagaaa
     3781 ttgtcaagct agaggatgtt ttgcatctct atcaggctgt cggttggaca aattataccc
     3841 atcaacctca gatgctggag caggccttgt ctcattcatt agtaatttat ctggcgcttg
     3901 atggcgatgc tgtggtgggc ttgattcgtt tggttggaga tggtttttca tcagtttttg
     3961 tacaggattt gattgtttta cctagctatc agcgccaagg gattggtagc tccttgatga
     4021 aacaggcttt agaggatttt aaagaggctt atcaagtcca gctggcgaca gaacagacag
     4081 aaaaaaatgt aggattttat cgttctatgg gttttgaaac tttatccacc tatgattgta
     4141 caggaatgat ttgggtaaac aaagaaaaat aacaaaactt gttttttctt aagcaaagtt
     4201 taaggatggt ctagtattat atagtcatta aataaagacc tcctaacttt atttaataa
//
LOCUS       DAVKWZ010000098         4240 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000098 DAVKWZ010000000
VERSION     DAVKWZ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.098"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            1..478
                     /locus_tag="VW854_002017"
                     /pseudo
     CDS             1..478
                     /locus_tag="VW854_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001822207.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            406..858
                     /locus_tag="VW854_002018"
     CDS             406..858
                     /locus_tag="VW854_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263191.1"
                     /translation="MEFNINSKESSQQLDILYKEVGVLSLGQKVVAMLDFLLAYSDYS
                     KDFRPLIIDRPEDNLDNRYIYRHLVQQFRDVKAQRQIILATHNATIVTNSMTDQVVIM
                     ESDGVNGWIESQGYVSEKYIKNHIINQLEGGKDSFKHKMSIYETALSE"
     gene            1677..2036
                     /locus_tag="VW854_002019"
     CDS             1677..2036
                     /locus_tag="VW854_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263192.1"
                     /translation="MSEQYRDIRKEVNLTADELKQIEKMMEVDNYRHFSPFVRDKILM
                     TDDKQLAAKEWFSLWQSQKFEQISRDVHLVLIIARENHQVTQEHVSILLTCVQELIAE
                     VNQVQSLSRGFREKYMR"
     gene            2046..2411
                     /locus_tag="VW854_002020"
     CDS             2046..2411
                     /locus_tag="VW854_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEV0263193.1"
                     /translation="MVYRYRTNLKKVFLTDSELHQLNERIAKSNCQNFSVYARKVLLN
                     PNMSFVTINTDTYEQLVFELRRIGNNINQIARAINQSHLISQDQLQELSKGVGELIKE
                     VDKEFQVEVKRLKEFHGSH"
     gene            2398..>4240
                     /locus_tag="VW854_002021"
     CDS             2398..>4240
                     /locus_tag="VW854_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEV0263194.1"
                     /translation="MVVTKHFATHGKKYRRRLIKYILNPDKTDNLKLVSDFGMSNYLD
                     FPSHAEMVEMYNVNFSNNDKLYESRNDRQEKHQQTIHAHYLIQSFSPEDNLTPEEINR
                     IGYEIMMELTGGRFKFIVATHTDKDHVHNHILINAIDRNSDKKLIWNYALERNLRMIS
                     DRISKVTGAKIIEKRYSYRDYKKYRESSHKFELKQRLYFLLQQSKSFDDFLEKAKQLH
                     VQIDFSQKHSRFLMTDRTMIKPIRGRQLSKRDLYDEDFFKTYFAKQEIESRLKFLLKS
                     VYSLEELHVKAKELNLTIELKQKNVMFTLEEDGKKISLSHKKISDKKLYDVQFFNRYF
                     EDREVRDIQALENLQEDFQTFREEQHKEKVSAEEIEEAFKKYKEKRDAIHEFEIELTD
                     NQIEKLVDDGVYIQVSFGIKQSGLIFIPNYQLDIFEEDNHKKYKVYIRETSSYFVYNK
                     ENMDNNCFIKGRTLIRQLSNDSQKLPYRRPTLKSLKEKISEINLMIELSNTNKQYQEI
                     KDELVLEIAEIDMQLEETQEKIATLNKMAEVLINLKSEDHETRKLAKYDFDQMNMTES
                     IMLDRLNTDILKLQQELGNEINKYEEIARRLDLFVKIINTNKFTVLKF"
BASE COUNT         1547 a          632 c          833 g         1228 t
ORIGIN      
        1 atcataaaaa atggaataga aatagttgta gtaactgatc ataatactac caaaggtatt
       61 aaaaagttac aaatggcagt ctcaatcata atgaaaaatt atccgattta tgatatacat
      121 cctcatattt tacatggagt agaaattagt gcagcagata aattgcatat tgtatgtata
      181 tatgattatg aacaagaatc atgggttaat caatggttaa gtgaaaatat tataagtgag
      241 aaagatggaa gttatcaaca ttcactgact ataatgaagg atttcaataa tcaaaaaata
      301 gttaactata ttgctcattt caatagttat gacattttga aaaaaggttc tcacttatca
      361 ggtgcatata aacgaaaaat tttttctaaa gaaaatacac gatttttgga gtttaatatt
      421 aactcgaaag aatcttcgca acaacttgat attctctata aagaagttgg tgtattaagt
      481 ttgggacaaa aagttgtagc catgcttgat tttttattag catatagtga ttattctaaa
      541 gacttcagac cattgattat tgatcggcct gaagacaatc tagacaatcg ttatatttac
      601 aggcatttag ttcagcagtt tagagatgtg aaagctcaac gtcaaattat tttagcaaca
      661 cataatgcta caattgtaac aaattctatg acagatcaag ttgttattat ggagtcagat
      721 ggagttaacg gatggattga atcacaggga tatgttagtg aaaaatatat aaaaaatcat
      781 atcatcaatc aattagaggg aggaaaagat tccttcaagc ataaaatgtc tatatatgag
      841 acggctttat cagagtagag tcagaaaaag taggttagaa atttagccta cttttttctt
      901 tgtccgacag gcatagtgta catctgaggt ccaagtcctc tgtggatgtt tgctgcagat
      961 gaaaccaata gcgactccta agcctgaata tcgtgaggta ggggggatag gaaggaatta
     1021 gcgaaatcaa ggttctacaa acagaatcgc gacttgaagc catatatagc ggatgaggaa
     1081 ctctaaaatc caaataggtg tcgtaaccta tatacgtaaa ttacgagagt aaactaggaa
     1141 agatgtacgg cttattccgt gagcgtttag gacgtagtac aacgaatcat gggagtcagc
     1201 tgaacacata gtattgaaga aatttctgta atggaaatgg agcgaagaag tgaacgatta
     1261 aatgaatacc tctctaatta aatttgtcaa ttctaattcc tggtatgaaa agacagtgac
     1321 ctgaaaatgt aaacgatggg agctgatcat aaatatagga cggtacatgc agtggtgtta
     1381 gagattagtc cttacttgat ttgtgataac ttccccaaat ttcttctgct atacttttct
     1441 caacttttaa aaatccaact aagaatttta cctgggggtt tgggggcgaa gccaccaagt
     1501 tatcttatcg ttggctgtca aaactggaag gttttggtta gctggcgata tgattttttg
     1561 ggtattgtga tcacaatgcc tgaactcgca aagaccgaac aaggacttta agaaatttcc
     1621 cgtatgacac acggggtcgg ggtttacccg agaaaggatg aaagtgagga aaagagatgt
     1681 ctgaacagta ccgtgacatt cgaaaagagg taaatctaac cgcagatgag ctaaaacaga
     1741 ttgaaaaaat gatggaggtt gataattatc gtcacttttc accattcgtc agggataaaa
     1801 ttttgatgac tgatgacaaa cagttagctg ccaaagaatg gttttccctt tggcagtctc
     1861 aaaagtttga acagatcagt cgagatgtgc atttggtttt gattatcgca agagagaatc
     1921 accaagtgac tcaagaacac gtgtcaatct tattgacctg cgttcaagaa ttgattgcgg
     1981 aagtcaatca agtacagtca ctcagtcgtg ggtttcgtga aaaatacatg agataggagg
     2041 ctactatggt ttatcgttat cgtaccaatc tcaaaaaagt atttctaaca gattcagaat
     2101 tacaccaatt gaatgaacgg attgctaaga gcaattgtca aaatttctca gtctatgcca
     2161 gaaaagtttt gcttaatccc aatatgtcat ttgtcacgat taacactgat acttatgaac
     2221 agttagtgtt tgaattgagg aggattggaa ataacatcaa tcaaattgcg cgtgcgatta
     2281 atcaaagcca tctgatttct caggaccaat tacaagaatt gagtaaagga gtcggagagt
     2341 tgatcaagga agtggacaaa gaatttcaag tggaagtgaa aagattgaag gagtttcatg
     2401 gtagtcacta agcattttgc gacacacgga aaaaaatatc gtaggcgttt aattaagtat
     2461 atcctcaatc ctgataaaac ggacaatttg aaattagtat ctgattttgg catgagcaat
     2521 tacttggact tccctagtca tgcagaaatg gtagagatgt acaatgtcaa cttcagcaat
     2581 aatgacaagt tgtacgaatc cagaaacgac cgacaagaaa aacaccaaca aactattcat
     2641 gctcattacc tcattcaatc attttctcct gaagataatc tgacacctga agaaattaac
     2701 cgcattggtt atgagatcat gatggaattg acaggaggtc gttttaagtt tatcgtagca
     2761 actcacacag acaaagatca tgttcataat cacatcctaa tcaacgctat tgaccgcaat
     2821 tcagataaaa agttgatatg gaactatgcc ttggaacgaa acctccgtat gatttcagac
     2881 cgtatttcta aagtgactgg tgcaaaaatt attgagaaac gttattccta tcgagattat
     2941 aaaaaatata gggaatctag tcataagttt gaattgaaac aacgtcttta ttttttgcta
     3001 cagcaatcta aatcgtttga tgatttttta gagaaagcaa agcagttgca tgttcagatt
     3061 gattttagtc aaaagcatag ccgttttttg atgacagata gaacgatgat aaaaccaatt
     3121 cgaggacgcc aactcagcaa acgagattta tatgatgaag acttttttaa aacatatttt
     3181 gccaaacaag agattgaaag tcgtttaaaa tttttactaa agtctgttta ttcattagaa
     3241 gaattgcatg taaaggctaa agagttgaat ctaaccatcg agttaaaaca gaaaaatgtg
     3301 atgtttaccc tagaagaaga tggcaaaaag ataagcctga gtcataaaaa aattagcgat
     3361 aagaaattgt acgatgttca attttttaat cgttattttg aagatagaga agttagagat
     3421 attcaagcat tagaaaatct tcaggaagat tttcagactt ttcgagagga gcagcataag
     3481 gagaaagtct ccgctgaaga aatcgaggaa gccttcaaga aatacaagga aaaacgagat
     3541 gctatccatg agtttgagat tgaacttacg gataatcaga tagagaagtt ggttgatgat
     3601 ggtgtctata tacaggtttc gtttggtatc aaacagagtg gactcatctt tattcctaac
     3661 taccagctag atatttttga ggaagacaat cataaaaaat ataaagtcta cattcgagaa
     3721 acgtcttctt actttgtcta taataaagaa aacatggata ataattgttt catcaagggt
     3781 aggaccttaa tcagacaatt gagcaacgat agccaaaaac tcccctatag acgaccaact
     3841 ttaaaaagtc ttaaagagaa gatttctgaa attaatctta tgattgaact atctaacacg
     3901 aacaaacaat accaagaaat caaagatgag cttgtattag aaattgcaga gattgatatg
     3961 cagctagaag aaacccaaga aaaaatcgcc accttaaaca agatggcgga agtccttatc
     4021 aatttgaaga gtgaagatca tgagactaga aaattagcta agtatgattt tgaccaaatg
     4081 aacatgacgg aatcaataat gttagatcga ctcaatacgg atattttaaa attacaacag
     4141 gaacttggta atgaaattaa taaatatgag gagatagcta gaagattgga tttgtttgtg
     4201 aaaattatta ataccaataa atttacagtt ctaaaatttc
//
LOCUS       DAVKWZ010000099         4192 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000099 DAVKWZ010000000
VERSION     DAVKWZ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.099"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            720..1712
                     /locus_tag="VW854_002022"
     CDS             720..1712
                     /locus_tag="VW854_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV0263195.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPDSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            1714..2532
                     /locus_tag="VW854_002023"
     CDS             1714..2532
                     /locus_tag="VW854_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV0263196.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2534..3319
                     /locus_tag="VW854_002024"
     CDS             2534..3319
                     /locus_tag="VW854_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0263197.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            3417..3635
                     /locus_tag="VW854_002025"
     CDS             3417..3635
                     /locus_tag="VW854_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEV0263198.1"
                     /translation="MKDLPEWFGIPESTQTYIEGTTTLQVWTAYQESDLTGFVSLSYS
                     SEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            3620..3817
                     /locus_tag="VW854_002026"
     CDS             3620..3817
                     /locus_tag="VW854_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0263199.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEASNKDYDRTNDFYRGLGFKKL
                     EIFPQLWNPQNPCQILIKKLE"
BASE COUNT         1165 a          718 c          904 g         1405 t
ORIGIN      
        1 gctatatatt atggtatcga atcttcatca gaatgataaa attaatcaat tgatatctga
       61 ttacaaacag aatatgaaag ctttttatat cactattgaa aaatttatac gagatgatga
      121 aagccttaag tgttatttta taaaggttat ttcaagtcgt tccaaggtaa caagtctaga
      181 tcagattgaa gctgataaaa cgatacaaag aaaatattca agtgagctaa aaaaatttat
      241 tggattttat aatgagatta tttgtgagga aaatagtttc ctacatgtac gaaagaggtg
      301 gtcgagttgg tttaggtagt cgatgcgtga gttgataatt ctcagggtat ggacttcttt
      361 ttcatgaatg aggtaaaaga gcaggtattg tttagagaca atcattctga gcatattttc
      421 tggatagagg gagtatccga ttttatgatc aaagttaata ccgccctctg gtgagaagat
      481 gagtaggttg gtaatttaaa ctattaaaca gaatttttga ttaaaagtat tattttatga
      541 gagaaatcct aatttcacaa tccataggca aactcttgca tttcgttttt tattggacta
      601 taataggttg gtataaagcc ttttgtagta ataaaatgta gaaggtgtag aaagcaagga
      661 tttagaatat ttgtagttaa aaacacaatg ttgctattcc ttacgatagg gagatagata
      721 tggcaatgat agaagtggaa catcttcaga aaaattttgt gaagactgtt aaggaaccgg
      781 gcttgaaggg ggctttgcgc tcctttattc atcctgaaaa gcagaccttt gaagcggtca
      841 aggatttgac ctttgaggtt ccaaaagggc agattttagg atttatcggg gcaaatggtg
      901 ctgggaagtc gacaaccatt aaaatgctga caggaatttt gaaaccaaca tctggttttt
      961 gtcggattaa cggcaagatt ccccaggaca atcggcaaga ttatgtcaaa gatattggcg
     1021 tagtctttgg acaacgcacc cagctatggt gggatttggc tctgcaagag acctacactg
     1081 tcttaaaaga gatttatgat gtgccagact cgctctttca taagcgtatg gactttttga
     1141 atgaagtctt ggatttgaag gactttatca aggatcccgt gcggactctt tcactgggac
     1201 aacggatgcg ggcggatatt gcggcctcct tgctccacaa tcccaaggtt ctttttttag
     1261 atgagccgac cattggtttg gacgtttcgg ttaaggataa tattcgtcgg gcaattactc
     1321 agatcaatca agaggaagaa actaccattc ttttgaccac tcacgatttg agtgatattg
     1381 agcaactttg tgatcggatt ttcatgattg acaaggggca agagattttt gatggaacgg
     1441 tgagccaact caaggagacc tttggtaaga tgaagactct ctcttttgaa ctgctaccag
     1501 gtcaaagtca tctcgtctct cactatgaag gtctgtctga tatgaccatt gatagacaag
     1561 gaaacagcct caacattgaa tttgatagtt ctcgctacca gtcagctgac attatcaagc
     1621 aaaccctgtc tgattttgaa atccgcgatt tgaagatggt ggatacggat attgaggata
     1681 ttatccgtcg cttctaccga aaggagctct aggatgatca aattgtggag acgttataaa
     1741 ccctttatca atgcaggggt tcaggagttg attacttacc gagtcaactt tattctctat
     1801 cggattggcg atgtcatggg ggcttttgtg gccttttatc tctggaaggc tgtctttgat
     1861 tcttcgcaag agtctttgat tcagggcttc agtatggcgg atatcaccct ctacatcatc
     1921 atgagttttg tgaccaatct tctgactaga tccgattcgt cctttatgat tggggaggag
     1981 gtcaaggatg gttccattat catgcgtttg ttgcgaccag tgcattttgc ggcctcctat
     2041 cttttcaccg agcttggttc caagtggttg atttttatca gcgttggcct tccattttta
     2101 agtgtcattg tcttgatgaa aatcatatcg ggtcaaggta ttgtagaggt gctaggatta
     2161 actgtccttt atctttttag cttaacgctc gcctatctga ttaacttttt ctttaatatt
     2221 tgctttggat tttcagcctt tgtgtttaaa aatctttggg gttccaacct acttaagact
     2281 tccatagtgg cttttatgtc ggggagtttg attcccttgg cattctttcc aaaggttgtt
     2341 tcagatattc tttccttttt gcctttttca tccttgattt atactccagt tatgatcatt
     2401 gttggaaaat acgatgccag tcagattctt caggcactcc ttttgcagtt cttctggctc
     2461 ttagtgatgg tgggattgtc tcagttgatt tggaaacggg tccagtcctt tatcaccatt
     2521 caaggaggtt agtatgaaaa aatatcaacg aatgcatctg atttttatca gacaatacat
     2581 caaacaaatc atggaatata aggtggattt tgtggttggt gtcttgggag tctttctggc
     2641 tcaaggcttg aatctcttgt ttctcaatgt catctttcaa catattccat tcctagaagg
     2701 ttggaccttt caagagatag ctttcattta tggattttcc ttgattccca agggaatgga
     2761 ccatctcttt tttgacaatc tctgggcact agggcaacgc ctagtccgaa aaggagagtt
     2821 tgacaagtat ctgactcgtc ccatcaatcc tctctttcac atcctagttg aaacctttca
     2881 gattgatgcc ttgggtgaac tcttagtcgg tggtatttta ttgggaacaa cagtgaccag
     2941 cattgtttgg actcttccaa aatttctgct tttcctagtt tgtattcctt ttgcgacctt
     3001 gatttatact tctcttaaaa tcgcaacagc cagtatcgcc ttttggacta agcagtcagg
     3061 cgccatgatt tacatcttct atatgttcaa tgactttgct aagtatccga tttctattta
     3121 caattctctt cttcgttggt tgattagctt tatcgtgcct ttcgccttta cagcctacta
     3181 tccagctagc tatttcttac aggaaaagga tgtgttcttt aacgtaggag gtttgatgtt
     3241 gatttctctg gttttctttg ttatttccct taaactttgg gataagggct tagattccta
     3301 cgaaagtgcg ggttcgtaaa agctaaagta agactaaaat caagaaagga acttatgatg
     3361 tttgtaattg aagaagtcaa ggatgaaaat caaaaaaggc agttgtcgct gagattttga
     3421 aggatttgcc agaatggttt ggaatcccag aaagcacaca aacctatata gaaggaacca
     3481 cgacactgca agtttggacc gcctatcagg agagtgattt gactggattt gtaagcttat
     3541 cctattcgag tgaagattgt gcagagattg attgtctcgg cgtaaaaaag cttatcaagg
     3601 tagaaaaatt gggagccaat tgcttgctac tttagagagt gaagctcgta aaaaagttgg
     3661 ttatctgcag gtcaaaacag tggcagaagc ttctaataaa gattatgatc gaacaaatga
     3721 cttttatcga ggtcttggct ttaaaaagtt agagattttt cctcaactat ggaatccgca
     3781 aaatccttgt cagattttga ttaaaaagct tgaataatat tacttgacat ctatttttta
     3841 ttctcagagt gctatactgt aagtgtaatc gccgatttag ctcagttggt agagcaaggc
     3901 actcgtaaag cctaggttat aggtagataa acgactgagg agttgaaaaa atagataggt
     3961 agaagataac cgttaagcct tattcttagc ggttatttat attgtttaat agcgctgata
     4021 tttcatcaat tatgcctgtt ttcgtgtttc cggtagttgt tcaagtttat tgctactatt
     4081 tttgatggta tgaatgtgct tataatgtat cccggttaac gaaagttttg gacttatact
     4141 cttagaaaat ctcttcaaac cacgtcaacg ccgccttgcc gtgcgtatgg tt
//
LOCUS       DAVKWZ010000100         4067 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000100 DAVKWZ010000000
VERSION     DAVKWZ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.100"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            <1..340
                     /locus_tag="VW854_002027"
     CDS             <1..340
                     /locus_tag="VW854_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEV0263200.1"
                     /translation="DSLDNRAIYSELTKYIKKTKKNRQIIIVTHNPNIVVSGDAENII
                     VANQQSDNSPNQNGKKFDYVNGALENRNNDSTSEFILQKYNIREHVCDILEGGEDAFI
                     KRENKYSING"
     gene            473..805
                     /locus_tag="VW854_002028"
     CDS             473..805
                     /locus_tag="VW854_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEV0263201.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDNFLQEQIEI
                     LTSIDNRE"
     gene            817..2388
                     /gene="ffh"
                     /locus_tag="VW854_002029"
     CDS             817..2388
                     /gene="ffh"
                     /locus_tag="VW854_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEV0263202.1"
                     /translation="MAFESLTERLQNVFKSLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRLAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(2583..3866)
                     /locus_tag="VW854_002030"
     CDS             complement(2583..3866)
                     /locus_tag="VW854_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEV0263203.1"
                     /translation="MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLEANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLIGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
BASE COUNT         1340 a          729 c          906 g         1092 t
ORIGIN      
        1 agatagtttg gataataggg caatatatag tgaattgaca aaatacatca aaaaaactaa
       61 aaagaatcga caaataatca tagttactca caatcctaat atagttgtat ctggtgatgc
      121 agaaaatatt attgtagcta atcagcaatc tgataattct ccaaatcaaa atggtaagaa
      181 gtttgattat gtaaatggtg ctttagagaa ccgtaataat gattctacat ctgaattcat
      241 acttcaaaaa tacaacatca gggaacatgt atgtgatatt ttggaaggtg gagaagacgc
      301 ctttattaag agggaaaata aatacagtat taatggctaa taataattgt attaattaaa
      361 aatggagagc attaatttat aaaagacaca tatttagttc tagcaaacag tctttttaag
      421 gagttaggat tggtaattgg actgccgggt aatgtgtggt ataataaaag ttatggaaat
      481 cgaaaaaacc aatcgtatga atgcgctctt tgaattttat gcggcgcttt tgacagataa
      541 gcaaatgaat tatatcgagc tctactacgc tgatgattac agccttgctg aaattgccga
      601 ggagttcggt gtcagtcgtc aggctgtcta tgacaatatc aagcgaacag aaaagattct
      661 ggaagattat gagatgaaat tgcacatgta ctcggactat attgtccgca gtcagatttt
      721 tgatcagatt ttggagcgct atcccaagga taactttctg caggagcaga tagaaatttt
      781 aacaagcatt gataatagag aataagagga agaaaaatgg catttgaaag tttaacagaa
      841 cgtttgcaga acgtctttaa aagtctacgt aaaaaaggaa aaatctctga atctgatgtc
      901 caagaggcaa ccaaagaaat tcgcttggcc ttgctcgagg ccgacgttgc cttgcctgtt
      961 gtaaaggact ttatcaagaa agttcgtgag cgtgcagtcg ggcatgaggt cattgataca
     1021 cttaatcctg cgcaacagat tattaaaatc gttgatgagg aactgacagc tgttttaggt
     1081 tctgatacgg cagaaattat caagtcacct aagattccta caatcatcat gatggttggt
     1141 ttgcagggag ctggtaaaac aacttttgca ggtaagctgg ctaacaaact caagaaggaa
     1201 gaaaatgccc gtcctttgat gattgcggcg gatatttacc gtcttgctgc cattgatcag
     1261 ctgaagacac tgggacaaca gattgatgtg ccggttttcg ctcttgggac agaggttcca
     1321 gctgttgaga ttgtacgtca aggtttggag caagctcaaa ctaatcataa cgactatgtc
     1381 ttgattgata ctgcgggtcg tttgcagatt gatgagctcc tcatgaatga gcttcgtgat
     1441 gtgaaagcat tggctcaacc aaatgaaatc ttgcttgtcg ttgatgctat gattggtcag
     1501 gaagcagcca atgttgcgcg tgagtttaat gctcagttgg aagtgactgg ggtcatcctt
     1561 accaagattg atggcgatac tcgtggtggt gctgctctgt ctgttcgtca cattactgga
     1621 aaaccaatca agttcactgg tacaggtgaa aaaattacag atatcgaaac cttccaccca
     1681 gaccgtatgt ctagccgtat ccttggcatg ggggatatgc tcactttgat tgagaaagct
     1741 tctcaggaat acgatgaaca aaaagccctt gaaatggctg agaagatgcg cgaaaacacc
     1801 tttgatttta atgatttcat cgatcaatta gatcaggtgc aaaatatggg gccgatggaa
     1861 gacttgctca agatgattcc aggtatggcc aacaatccag cccttcaaaa catgaaggtg
     1921 gatgaacgcc agattgctcg taaacgtgcc attgtgtctt cgatgacacc tgaagagcgt
     1981 gaaaacccag atttgttaaa tccaagccgt cgccgtcgta ttgctgctgg ttctggaaat
     2041 acattcgtcg aagtcaataa attcatcaag gactttaacc aggctaaaca gctcatgcag
     2101 ggtgttatgt ctggggatat gaataaaatg atgaagcaaa tggggattaa tccaaataac
     2161 cttcctaaaa atatgccaaa tatgggagga atggatatgt ctgcccttga aggaatgatg
     2221 ggacaaggcg gtatgcctga cttatcagct ctcggaggag caggaatgcc agatatgagc
     2281 cagatgtttg gtggcggttt gaaaggtaaa attggtgaat ttgccatgaa acagtccatg
     2341 aaacgtatgg ctaacaaaat gaagaaagcg aagaagaaac gcaagtaaga caagagattg
     2401 atgcaaatct catttgatga gaaaataaag taaaaatttc tatgataaaa cgtacagtat
     2461 caaggtttta gaatatctga tattatacgt tttttgattt taataatttt gttaagacac
     2521 ttttattata gtatacgaaa aatccactca gctgtctgag tggattaggt gtatttagac
     2581 tcttagtctt tattttcgta tggcaagatc aagttcaaga tgattcctgt catggctgaa
     2641 agggcagtac ctgaaagtgt aactggacca agtttaagga tagctcctcc aagtccaaga
     2701 accaacatag cacttgcgat gatgaggttt cgcatttgag cgaaatcaac acgttcttta
     2761 atcaagactt tcaaaccatt gctggcgata accccataga gaaggattga cataccacca
     2821 agtacagcgt ttggaatagt tgaaatcaag gcagtgaatt taccaaggaa gctgagggca
     2881 atcgcgatga aggcagcgtt acggataact gagacagaag cgatacgagt cataccgata
     2941 acccctgtat tttctccgta agttgtattg gctggtccac caaggaaggc agaaacagaa
     3001 gttgcgatac cgtcaccaat aagagtacgg tgaagacctg gttcttttaa gaattgacgg
     3061 ccacagattt gacccaaaac agtatggtct ccgatatgtt cagaaattgt tacgatagcg
     3121 attggcaaga tagcgatggc ttctggacca aagtaaagat tgtactcttt aaaggcacca
     3181 cctgtgctaa atggcaagta gaaaccagga atttcaaacc agttagcttc taggacaggc
     3241 gtaaagtcaa ccaaaccaag agttagtgcg aagaggtaac caccgataat ggcaaagagg
     3301 aatggaatga ttcgtaggaa gccttttcct tttgtattga taaaggcagc aattaggaaa
     3361 gtaacaacgg ctaccagagc atttttccaa tttccgtctg ctacaagacc tgcattggta
     3421 acagctgaac ctgcaagtcc aagaccgata acgatgatca taggaccgat aatgattggt
     3481 ggcaagagtt tatcaatcca ttttgttcct acaaatcgga tgctggtagc aacaaggaca
     3541 tagaccaaac cagtcaagat aacccctgtt tgagcagcag atacatcccc ccccatttct
     3601 ttcatagcca gtgacatagc tgtgataaag gcgaatgaag aacctagata aactggaact
     3661 ttaaaaccag tagcaatcat gtagatgagt gttccaacac ctgaagcaaa aagggcaaca
     3721 gatacaggca ttcccaaaat caatggtacc aagatggtcg caccaaacat ggcgaaaacg
     3781 tgttggaaac taaggagaat tccttttcca gccgaaggac gttggtcaac gtctagtaac
     3841 aaatcaacag ttgattcttg tttcatataa acctcacttt tgggcatcaa aaaagagccc
     3901 attattttac agcaataggc cctcagagta agacttctct aaaatctaga ctttaaagag
     3961 tttcaaatat ttttgcgtct tcgtcacctc acgggatgac attaaaaacc tttgcttagg
     4021 atagtatagc agaaaaaaat gattttgtaa atcatttttt cccgagc
//
LOCUS       DAVKWZ010000101         3879 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000101 DAVKWZ010000000
VERSION     DAVKWZ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.101"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            8..622
                     /locus_tag="VW854_002031"
     CDS             8..622
                     /locus_tag="VW854_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0263204.1"
                     /translation="MKLKKTCVFQNLNFSCKQGEIIGITGANGSGKSVLFKLIAGLYS
                     PSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLASIRGVVGNQEIN
                     DTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMNALDKSSVENMR
                     TLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(709..1580)
                     /locus_tag="VW854_002032"
                     /pseudo
     CDS             complement(709..1580)
                     /locus_tag="VW854_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
     gene            complement(1592..2350)
                     /locus_tag="VW854_002033"
     CDS             complement(1592..2350)
                     /locus_tag="VW854_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV0263205.1"
                     /translation="MSETILEIKELKKSFGDNPILQGLSLEIKKGEVVVILGPSGCGK
                     STLLRCLNGLESIQGGDILLDGQSIVENKKDFHLVRQKIGMVFQSYELFPHLDVLQNL
                     ILGPIKAQGRDKKEVTEEALQLLERVGLLDKQHSFARQLSGGQKQRVAIVRALLIHPE
                     IILFDEVTASLDPEMVREVLELINDLAQEGRTMILVTHEMQFAQAITDRIIFLDQGKI
                     AEEGTAQAFFTNLQTKRAQEFLNVFDFSQFGSYL"
     gene            complement(2351..2638)
                     /locus_tag="VW854_002034"
     CDS             complement(2351..2638)
                     /locus_tag="VW854_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV0263206.1"
                     /translation="MTNVQLYYHIIIPQVLRRLLPQAINLVTRMIKTTSLVVLIGVVE
                     VTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTHLEKHWRN"
     gene            complement(2648..3028)
                     /locus_tag="VW854_002035"
     CDS             complement(2648..3028)
                     /locus_tag="VW854_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154138.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV0263207.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIGISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLDFIRIMPQLVLLFIVYFGLARNFNINISGETSAIIIFTLW
                     GTAEMGDLVRGAITSLPKHQFESG"
     gene            complement(2988..3668)
                     /locus_tag="VW854_002036"
     CDS             complement(2988..3668)
                     /locus_tag="VW854_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV0263208.1"
                     /translation="MDWSIVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHY
                     RIPVLAQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMA
                     ESFRSGLETISQTQQEIGLAIGLTPLQVFRYVVLPQATAVALPSFSANVIFLIKETSV
                     FSAVALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRTRHAGFG
                     NPSTLSRK"
BASE COUNT         1195 a          703 c          848 g         1133 t
ORIGIN      
        1 tattgagttg aaattaaaaa aaacatgtgt ttttcaaaac cttaacttta gttgtaaaca
       61 gggggaaatt ataggaatta ctggtgcgaa tggctcaggg aaaagtgtat tgtttaaatt
      121 aatagctggt ttatatagtc cgtcttatgg agaagtgtta atcaatgggg aaaatattgt
      181 tcctgagaga aaaattccag ctaatttggg agctttgatt gaagaacctg gttttataaa
      241 ttattatagt ggctttaaga atttacaata tttggcaagc atacgaggag tagttggtaa
      301 tcaggaaatc aatgatacac tgaaaatagt tggtctatat gagcaaaaag accagaaagt
      361 taaaacttat tcgctaggta tgaggaaaaa gctagggatt gctcaagcaa ttatggagaa
      421 tccctctatt cttttactag atgaacctat gaatgccttg gataaatcaa gtgtagaaaa
      481 tatgagaaca ttgtttagaa agctctctag tgaaaaaggg acaacaattt tgattgctag
      541 tcatagtgaa gaggatattc gtatcttatg tgataaagta tatgcaatag aagataaagt
      601 atgtacactg tgttcagatt gaacatctta gatagactca ggatgttata ggtaaaatct
      661 taaaaaggga ttggagctta aaatccagtt ccttttaaga gttaatgact aatcaacttt
      721 tccaccttca acaaccaggt catctgcttt agcagcgtca ccgtaggttg ggtgaagtgt
      781 tttttcatag gccttgtgga agaagttttc cttgcctaat ttttcaatat ctttattgat
      841 gaagtctagc aattcttggt tgtctttttg aactgctgcc gcaatggtat cgggatcacc
      901 gagggaagta attcctactt caaatccttt attttcaagc gcccaagcta gaacttccgt
      961 attgtcagtt gaaaaggcat ctccacgtcc gtcaagaaga gcttggtaag agtcactgta
     1021 ttggtcgtat ttttggagtt tgatttctgg atgattcttt tcaaaataag tctcagcagt
     1081 cgttcctttt gtgacaatta aggttttacc ttcaagttgt ttgacgtctg taatgagacc
     1141 agtcttaggt gatacgacac ccagagaaac tttcatatat ggaagggcaa aatcaacttg
     1201 tttcttacgt tcgtcagtta ctgtaaagtt agcaagagta atatctacct tgtttgaaat
     1261 caagtattcc gcacggttgg cggcatcgac tgaaatgtat ttaaccttga caccaaggtc
     1321 ttgagctagt tggttcccta gttcaatatc gtagcgtagc cttggtaaga accatcattg
     1381 tcaacgtagc caaacggttt tttatctcca aacacggcga ttcgcagttc accgcttttt
     1441 ttgatttcat cgatagtgcg agctttggca gttgtttttc cagaggatga accagcgttt
     1501 ccacctgagc tacaagcagt gataaagata agggcaaagg caagtgctaa aacagttaag
     1561 agtggtttga atagtttcat aagaatctcc tctatagata tgagccgaat tggctaaagt
     1621 caaagacgtt taaaaattcc tgggctcgtt tggtttgcag attggtaaag aaggcttgag
     1681 ctgttccttc ttcagcgatt ttcccttggt cgaggaagat aatccggtca gtaatggctt
     1741 gggcaaactg catttcgtgg gttactaaaa tcatggtacg gccttcttgg gccaaatcat
     1801 tgataagttc cagcacctca cgcaccattt ctggatccag cgaagcagtc acctcgtcaa
     1861 aaaggatgat ttctggatgt attaggaggg cacggacaat tgcaacacgt tgcttctgtc
     1921 caccagataa ttgacgggca aagctatgtt gtttatccag caaaccgaca cgctctagta
     1981 attgcaaagc ttcttccgtt acttctttct tgtcccttcc ttgagctttg atagggccta
     2041 ggatgaggtt ttgtaagaca tccagatggg gaaagagttc ataactttga aagaccatgc
     2101 caatcttttg gcgaactagg tgaaaatctt ttttattttc aacgatagac tgaccatcca
     2161 gaagaatatc tccaccttga atactttcta agccgttgag gcaacgaagg agggtacttt
     2221 tcccacaacc agatggcccc aggatgacaa caacttcccc ttttttgatt tctagagaaa
     2281 gtccttggag gatgggattg tctccgaagg atttttttag ttccttgatt tctaagatag
     2341 tttcagacat ttagtttctc caatgttttt ctaagtgagt ggatagtttg gaaatagggt
     2401 agcaaactgc gaaatataag actagaatgg ttccataaat ccaaaatgaa gcagttggga
     2461 tggtcaggcg attgctatcg atgatttgtt gtccaacttt ggtcacttcc acaaccccaa
     2521 tcaaaacaac taatgaagtg gttttaatca tccgagtgac aagattgata gcctgcggta
     2581 gcagtcttct taagacttgt gggatgatga tgtggtagta aagttgaaca ttagtcaagc
     2641 cgagtgccta tccactttca aactgatgtt tagggagaga agtgatagct ccacgtacca
     2701 agtctcccat ttcagctgtt ccccagaggg taaaaatgat aatagctgaa gtctcacctg
     2761 agatattgat attaaagttt cgagccaagc caaagtaaac gatgaagagt agcaccagct
     2821 ggggcatgat acggataaag tccagataca atcgtgttaa aaatcgtatg attctagaat
     2881 gggaggtcat gatgattccc atgactgttc cgaacatcat ggataagagg acagacagga
     2941 tggatatccc aatcgtaacg cccaacccct gtaagattct caggagatta tttccttgaa
     3001 agagtacttg gattcccgaa tcctgcatgg cgggtccttc tttctatcca gctaaagacc
     3061 agtgagatgg gtagcagcat gatcagataa gcaactacca acatagctag cgcaatgtct
     3121 gtctcatagt agagaccaat caaatccttg gcgacgtaca tgaggtcggc caaagccact
     3181 gctgagaaaa cagaggtttc cttgataagg aaaatgacat tggcactaaa ggaggggagt
     3241 gccaccgctg ttgcttgcgg aagaaccaca tagcgaaaga cctgtagagg tgtcagacca
     3301 atagcgaggc caatctcctg ctgggtttga ctgatggttt ccagcccact tcggaaagat
     3361 tctgccatat aggagcctcc taaaaagaca agcccaagcg ttgcacagac ttctgaagat
     3421 aggacaatgc cgattcgggg aagaccgaag tagagaaaga agagttgaat caaaaggggc
     3481 gtattacgtg acaattcaat gtaggctgtc gctacttgcg ccaaaacagg gattcgataa
     3541 tgtcggatga tactaacgat taaaccgagc agaaaggatc ccaaaattcc ccaaactgca
     3601 atatgcaagg tcagaaagaa tgccttttga tatagtggta gatattgttc aacaatggac
     3661 caatccaaaa atagaacctc ccatctagaa ataatacagt tattgtagca cttaaaatct
     3721 tctttggata atatctattt ttattgccgt tataaggatt tttttaccat agacataaaa
     3781 tttctgaaat ttccaaacaa aatatttgaa aagttttgaa aaaagagtta agatattttt
     3841 ataatataca aagtaaacgc ttacttatta aggaggaca
//
LOCUS       DAVKWZ010000102         3766 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000102 DAVKWZ010000000
VERSION     DAVKWZ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.102"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            150..377
                     /locus_tag="VW854_002037"
     CDS             150..377
                     /locus_tag="VW854_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263209.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            665..3562
                     /locus_tag="VW854_002038"
     CDS             665..3562
                     /locus_tag="VW854_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEV0263210.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTAE
                     LPNGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDKVVISNLRDNPEAKD
                     PAAPSPLNIDMVLVQDPTTKRIFSIYDMFPEGRAVFGMPKTPEKAYEKIGDKTYQILY
                     KQGESGHYTVRENGEVYNAQNQKTDYRVVVNPTEPGYRDKGNLYKGQELIGNIYFAHS
                     TKNPFRVANTSYLWMSYSDDDGKTWSAPRDITPGLRKDWMKFLGTGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NERAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHEGKEYIILSNAGGPKRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWDFLSKDLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
BASE COUNT         1303 a          601 c          852 g         1010 t
ORIGIN      
        1 aatgaggtat aataaattta ctaacactta taaaaagtga tagaatctat ctttatgtat
       61 atttaaagat agattgctgt aaaaatagta gtagctatgc gaaataacag atagagagaa
      121 gggattgaag cttagaaaag gggaataata tgatatttaa ggcattcaag acaaaaaagc
      181 agagaaaaag acaagttgaa ctacttttga cagttttttt tgacagtttt ctgattgatt
      241 tatttcttca cttatttggg attgtcccct ttaagctgga taagattctg attgtgagct
      301 tgattatatt tcccattatt tctacaagta tttatgctta tgaaaagcta tttgaaaaag
      361 tgttcgataa ggattgagca ggaagtatgg tataaatagc ataagctgat gtccatcatt
      421 tgcttataaa gagatatttt agtttaattg cagcggtgtc ctggtagata aactagattg
      481 gcaggagtct gattggagaa aggagagggg aaaattggca ccaatttgag atagtttgtt
      541 tagttcattt ttgtcattta aatgaactgt agtaaaagaa agttaataaa agacaaacta
      601 agtgcatttt ctggagtaaa tgtcttattt cagaaatcgg gatatagata tagagaggat
      661 cagtatgaat cggagtgttc aagaacgtaa gtgtcgttat agcattagga aactatcggt
      721 aggagcggtt tctatgattg taggagcagt ggtatttgga acgtctcctg ttttagctca
      781 agaaggggca agtgagcaac ctctggcaaa tgaaactcaa ctttcggggg agagctcaac
      841 cctaactgat acagaaaaga gccagccttc ttcagagact gaactttctg gcaataagca
      901 agaacaagaa aggaaagata agcaagaaga aaaaattcca agagattact atgcacgaga
      961 tttggaaaat gtcgaaacag tgatagaaaa agaagatgtt gaaaccaatg cttcaaatgg
     1021 tcagagagtt gatttatcaa gtgaactaga taaactaaag aaacttgaaa acgcaacagt
     1081 tcacatggag tttaagccag atgccaaggc tccagcattc tataatctct tttctgtgtc
     1141 aagtgctact aaaaaagatg agtacttcac tatggcagtt tacaataata ctgctactct
     1201 agaggggcgt ggttcggatg ggaaacagtt ttacaataat tacaacgatg cacccttaaa
     1261 agttaaacca ggtcagtgga attctgtgac tttcacagtt gaaaaaccga cagcagaatt
     1321 gccaaatggt cgagttcgcc tgtatgttaa tggtgtttta tctcgaacaa gtctgaaatc
     1381 tggcaatttc attaaagata tgccagatgt aacgcatgtg caaatcggag caaccaagcg
     1441 tgccaacaat acggtttggg ggtcaaatct acagattagg aatctcactg tgtataatcg
     1501 tgctttaaca ccagaagagg tacaaaaacg tagtcaactt tttaaacgct cagatttaga
     1561 aaaaaaacta cctgaaggag cggctttaac agagaaaacg gacatattcg aaagcgggcg
     1621 taacggtaaa ccaaataaag atggaatcaa gagttatcgt attccagcac ttctcaagac
     1681 agataaagga actttgatcg caggtgcaga tgaacgccgt ctccattcga gtgactgggg
     1741 tgatatcggt atggtcatca gacgtagtga agataatgga aagacatggg gagataaggt
     1801 ggttatctcc aatcttcgag ataatcctga agctaaagat cctgctgcgc catcgcctct
     1861 aaatattgat atggttttgg ttcaagaccc gacaacaaag agaatcttct caatttatga
     1921 tatgttccca gaaggtcgag cagtttttgg aatgccaaaa acacctgaaa aagcttatga
     1981 aaagataggg gataaaactt atcaaatctt gtataaacaa ggagagtctg gtcattatac
     2041 tgttcgtgag aatggagaag tgtataatgc acaaaatcaa aagacggatt atcgtgttgt
     2101 agtgaatcca acagaacctg gctatagaga taaaggaaat ctttacaaag gtcaggaatt
     2161 gattggaaat atctattttg cacacagtac aaaaaatcca tttagagtag ccaatacgag
     2221 ctatctatgg atgtcatata gtgacgatga tggtaaaact tggtctgcac cgagagacat
     2281 tactccaggt cttcgcaagg attggatgaa gttcctagga acaggtcctg gaacaggaat
     2341 tgtacttcgg aatgggcctc acaagggacg gattttgata ccggtttata cgactaataa
     2401 tgtatctcac ttaaatggct cgcaatcttc tcgtgtcatc tattcagatg atcatggaaa
     2461 aacttggcat gctggagaag cggtcaacga taaccgtcag gtagacggtc aaaagatcca
     2521 ctcttctacg atgaacaatg aacgtgcgca aaatacagaa tcaacggtgg tacaactaaa
     2581 caatggagat gttaaactct ttatgcgtgg tttgactgga gatcttcagg ttgctacaag
     2641 taaagacgga ggagtgactt gggagaagga tatcaaacgt tatccacagg ttaaagatgt
     2701 ctatgttcaa atgtctgcta tccatacgat gcacgaagga aaagaataca tcatcctcag
     2761 taatgcaggt ggaccgaaac gtgaaaatgg gatggtccac ttggcacgtg tcgaagaaaa
     2821 tggtgagttg acttggctca aacacaatcc aattcaaaaa ggagagtttg cctataattc
     2881 gctccaagaa ttaggaaatg gggagtatgg tatcttgtat gaacatactg aaaaaggaca
     2941 aaatgcctat accctatcat ttagaaaatt taattgggac tttttgagca aagatctgat
     3001 ttctcctacc gaagcgaaag tgaagcgaac tagagagatg ggcaaaggag agatgggcaa
     3061 aggagttatt ggcttggagt tcgactcaga agtattggtc aacaaggctc caacccttca
     3121 attggcaaat ggtaaaacag cgactttcct aacccagtat gatagcaaga ccttgttatt
     3181 tgcagtagat aaggaagata tcggacagga aattattggt atagctaaag gaagcatcga
     3241 aagtatgcat aatcttcctg taaatctagc aggtgccaga gttcctggcg gagtaaatgg
     3301 tagcaaagca gcggtgcatg aagttccaga atttacaggg ggagttaatg gtacagagcc
     3361 agctgttcat gaaatcgcag agtataaggg atctgattcg cttgtaactc ttactacaaa
     3421 agaagattat acttacaaag ctcctcttgc tcagcaggca cttcctgaaa caggaaacaa
     3481 ggagagtgac ctcctagctt cactaggact aacagctttc ttccttggtc tgtttacgct
     3541 agggaaaaag agagaacaat aagagaagaa ttctaaacat ttgattttgt aaaaatagaa
     3601 ggagatagca ggttttcaag cctgctatct ttttttgatg acattcaagc tgatacgaaa
     3661 tcataagagg tatgaaacta ctttcagggt agtctgttct ataaaatata gtagattgaa
     3721 ataagatgtg aacaactcta ccaggaaagt caaattaatt tataga
//
LOCUS       DAVKWZ010000103         3736 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000103 DAVKWZ010000000
VERSION     DAVKWZ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.103"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            71..1252
                     /locus_tag="VW854_002039"
     CDS             71..1252
                     /locus_tag="VW854_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEV0263211.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLRSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            1310..2068
                     /locus_tag="VW854_002040"
     CDS             1310..2068
                     /locus_tag="VW854_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEV0263212.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKKQQDYFFQRIIEEDISVRKLETLLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            2281..3642
                     /gene="dnaA"
                     /locus_tag="VW854_002041"
     CDS             2281..3642
                     /gene="dnaA"
                     /locus_tag="VW854_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEV0263213.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
BASE COUNT         1325 a          648 c          669 g         1094 t
ORIGIN      
        1 tttttgtgtt taagttataa ttaagctagt cagtttatac tttcagtaat tcaaacatat
       61 ggaggcaaat atgaaacatc taaaaacatt ttacaaaaaa tggtttcaat tattagtcgt
      121 tatcgtcatt agctttttta gtggagcctt gggtagtttt tcaataactc aactaactca
      181 aaaaagtagt gtaaacaact ctaacaacaa tagtactatt acacaaactg cctataagaa
      241 cgaaaattca acaacacagg ctgttaacaa agtaaaagat gctgttgttt ctgttattac
      301 ttattcggca aacagacaaa atagcgtatt tggcaatgat gatactgaca cagattctca
      361 gcgaatctct agtgaaggat ctggagttat ttataaaaag aatgataaag aagcttacat
      421 cgtcaccaac aatcacgtta taaatggcgc cagcaaagta gatattcgat tgtcagatgg
      481 gactaaagta cctggagaaa ttgtcggagc tgacactttc tctgatattg ctgtcgtcaa
      541 aatctcttca gaaaaagtga caacagtagc tgagtttggt gattctagta agttaactgt
      601 aggagaaact gctattgcca tcggtagccc gttaggttct gaatatgcaa atactgtcac
      661 tcaaggtatc gtatccagtc tcaatagaaa tgtatcctta agatcggaag atggacaagc
      721 tatttctaca aaagccatcc aaactgatac tgctattaac ccaggtaact ctggcggccc
      781 actgatcaat attcaagggc aggttatcgg aattacctca agtaaaattg ctacaaatgg
      841 aggaacatct gtagaaggtc ttggtttcgc aattcctgca aatgatgcta tcaatattat
      901 tgaacagtta gaaaaaaacg gaaaagtgac gcgtccagct ttgggaatcc agatggttaa
      961 tttatctaat gtgagtacaa gcgacatcag aagactcaat attccaagta atgttacatc
     1021 tggtgtagtt gttcgttcgg tacaaagtaa tatgcctgcc aatggtcacc ttgaaaaata
     1081 cgatgtaatt acaaaagtag atgacaaaga gattgcttca tcaacagact tacaaagtgc
     1141 tctttacaac cattctatcg gagacaccat taagataacc tactatcgta acgggaaaga
     1201 agaaactacc tctatcaaac ttaacaagag ttcaggtgat ttagaatctt gattgacatc
     1261 tatgtaaaga aagctttaca taagagaaaa gatgtgttag tgtagaatca tggaaaaatt
     1321 tgaaatgatt tctatcacag atatacaaaa aaatccctat caaccccgaa aagaatttga
     1381 tagagaaaaa ctagatgaac tagcacagtc tatcaaagaa aatggggtca ttcaaccgat
     1441 tattgttcgt caatctcctg ttattggtta tgaaatcctt gcaggagaga gacgctatcg
     1501 ggcttcactt ttagctggtc tacggtctat cccagctgtt gttaaacaga tttcagacca
     1561 agagatgatg gtccagtcca ttattgaaaa tttacagaga gaaaatttaa acccaataga
     1621 agaagcacgc gcctatgaat ctctcgtaga gaaaggattc acccatgctg aaattgcaga
     1681 taagatgggc aagtctcgtc catatatcag caactccatt cgtttacttt ccttgccaga
     1741 acagattctc tcagaagtag aaaatggcaa actatcacaa gcccatgcgc gttccctagt
     1801 tgggttaaat aagaaacaac aagactattt ctttcaacgg attatagaag aagatatttc
     1861 tgtaaggaag ttagaaactc ttctgacaga gaaaaaacaa aagaaacagc aaaaaactaa
     1921 tcatttcata caaaatgaag aaaaacagtt aagaaaacta ctcggattag atgtagaaat
     1981 taaactatct aaaaaagaca gtggaaaaat cattatttct ttttcaaatc aagaagaata
     2041 tagtagaatt atcaacagcc tgaaataagg ctgttctttt atttttttat ctcacaaggt
     2101 tatccactat gtttttcgat aaaaagctta ataaatcaat aatttcttct tttatcccca
     2161 acctgtggat aaagtttggt aacattgtgg attatttttc acagcttgtg gaaaattctt
     2221 gctatctatg gtaaaatatc tctagtatta aacttttaaa tagtaaagga ggagaaagaa
     2281 ttgaaagaaa aacaattttg gaatcgtata ttagaatttg cacaagaaag actgactcga
     2341 tccatgtatg atttctatgc tattcaagct gaactcatca aggtagagga aaatgttgcc
     2401 actatatttc tacctcgctc tgaaatggaa atggtctggg aaaaacaact aaaagatatt
     2461 attgtagtag ctggttttga aatttatgac gctgaaataa ctccccacta tattttcacc
     2521 aaacctcaag atacgactag ctcacaagtt gaagaagcta caaatttaac tctttatgac
     2581 tatagtccaa agttagtatc tattccttat tcagatacgg gattaaaaga aaagtatacc
     2641 tttgataact ttattcaagg ggatggaaat gtttgggctg tatcagccgc tttagctgtc
     2701 tctgaagatt tggctctgac ctataaccct ctttttatct atggaggacc aggccttggt
     2761 aagactcact tattaaacgc tattggaaat gaaattctaa aaaatattcc taatgcgcgt
     2821 gttaaatata tccctgccga aagctttatt aatgactttc ttgatcacct aagacttggg
     2881 gaaatggaaa agtttaaaaa gacctatcgt agtcttgatc ttttgttaat cgatgatata
     2941 cagtcactca gcggaaaaaa agtcgcaact caggaagaat ttttcaatac ctttaacgcc
     3001 cttcatgaca agcaaaaaca gattgtccta acgagtgatc gtagtccaaa acatctagaa
     3061 gggctcgagg agaggcttgt cacgcgtttt agttggggat tgacacaaac tatcacaccc
     3121 cctgactttg aaacacgtat tgccatttta caaagtaaga cggaacattt aggctacaat
     3181 ttccaaagtg atactctaga atacctagct gggcaatttg attcaaatgt tcgagatctt
     3241 gagggagcca tcaacgacat cactttaatt gccagagtaa aaaaaatcaa ggatatcact
     3301 attgatattg ctgcagaagc cattagagcc cgcaaacaag atgttagcca aatgctcgtc
     3361 atcccaattg ataaaatcca aactgaagtt ggtaactttt atggtgttag tatcaaagaa
     3421 atgaagggaa gtagacgcct tcaaaatatt gttttggccc gtcaagtagc catgtattta
     3481 tctagagaac taacagataa tagtcttcca aaaattggga aggaatttgg gggaaaagat
     3541 cataccacag tcattcatgc ccatgccaaa ataaaatctt tgattgatca agacgataat
     3601 ttacgtttag aaattgaatc aatcaaaaag aaaatcaaat aatttgtgga taacttttag
     3661 ttttttatct tttttatcca cattttttaa acaagctaaa aaacttgata tgacttgttt
     3721 aaaggctgtt ttccac
//
LOCUS       DAVKWZ010000104         3696 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000104 DAVKWZ010000000
VERSION     DAVKWZ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.104"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            <1..747
                     /locus_tag="VW854_002042"
     CDS             <1..747
                     /locus_tag="VW854_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV0263214.1"
                     /translation="HLKSQLILPIVDKIIQTHNIKRIIFLGDYVDLHGQTNNIQLYAK
                     DLTFLYDWKIEKELNSIEVINLMGNHDVYYLLGEQVPFSIQNLEVFFSVQQLLQDLNL
                     QVAYQLDDYLVSHAGFNLLFDPKEWHFKPFTEEYEEELEILANAVGYMRGGGDMAGSP
                     LWAHFRELELIPNHNYPKQIVGHTPKESIDISKNVIGIDTFSLYIDKDNKYQFIGNGD
                     LLVYVQGHLEIFSTDWATDQTLKQLPKPQI"
     gene            931..1713
                     /locus_tag="VW854_002043"
     CDS             931..1713
                     /locus_tag="VW854_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HEV0263215.1"
                     /translation="MTSYNKKIVRTSSYIEIWEYEKPIFSKGKSILDTDTKSKEKSQR
                     RTFDELTPEEQEQRLERMKKTRLEAKWNLLRLVDCNYNDNTSFLTLTTKENISDRNHF
                     KDLLKTFIKRFNYHIFNTKKSKLKYLTVLEKQKRGAWHAHILLFSVPYVPHRQLLELW
                     GHGAVRINKVDVDSKENRGRYVTKYFEKGIGQELLENFGKQAYFSSRNLKKPDEDRFY
                     TYEDFNYDSSVVLYETEYISKVYKDGQYFDNHVKYKKIKLDE"
     gene            1727..1930
                     /locus_tag="VW854_002044"
     CDS             1727..1930
                     /locus_tag="VW854_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEV0263216.1"
                     /translation="MIATVTKNDLVALGFSEGTSKHIIRQGKELLITRGFTVYQNKRI
                     GTIPATIATELLGFDVQNSTLKE"
     gene            1934..3066
                     /locus_tag="VW854_002045"
                     /pseudo
     CDS             1934..3066
                     /locus_tag="VW854_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            3107..3544
                     /locus_tag="VW854_002046"
     CDS             3107..3544
                     /locus_tag="VW854_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HEV0263217.1"
                     /translation="MDTSQVIKAHEVAKKAHFGQIDRAGIDYIKHPEIVASFVATDEE
                     KAVAYLHDVIEDTSLTLLDLKKEGFSKNIIEAVDILTKKKGQDYQSYLNLVKKNELAR
                     VVKLADLRHNSDLTRLPLITEKDLERNKKYSSAIRFLRAKKKS"
BASE COUNT         1439 a          610 c          601 g         1046 t
ORIGIN      
        1 cacttaaaat ctcaactgat tttacccatt gttgacaaaa ttatccaaac acataatatt
       61 aagagaatca tttttcttgg tgattatgtt gatttacatg gacaaactaa caatatccaa
      121 ctatatgcca aagacctcac ttttctatac gattggaaaa tagaaaaaga attaaattca
      181 atagaagtta ttaaccttat gggcaatcac gatgtttatt atttacttgg agaacaagtt
      241 cctttctcaa ttcaaaatct agaggtcttt ttctcagttc aacaattatt acaagatttg
      301 aatttacaag tagcttatca actagatgat tatttagtta gccatgctgg tttcaatttg
      361 ctatttgacc caaaagaatg gcactttaaa ccttttactg aagaatatga agaagaatta
      421 gaaatccttg ctaatgcagt aggatatatg cgtggaggag gagatatggc tggttctcct
      481 ttatgggctc attttagaga actagagcta atccctaatc acaactaccc taaacaaatt
      541 gttggacata cccctaaaga atctatcgat atttctaaaa atgttattgg tatagatact
      601 ttttcattat atattgacaa agataataaa tatcaattta ttggtaacgg agatttgctc
      661 gtttatgtcc aaggacatct tgaaatcttt tcaactgact gggctacaga ccaaacatta
      721 aaacaattac ctaaacctca aatttaacta ctacttctca tcatgtgact aagcgaaact
      781 gccgactgga agtcggcaag ctggaagcgt gtttagcaga gcgatgctta gaaacacatg
      841 atagaagtat ggaggagctt tgcttctcct agtttattta ttatatagac aatcttttgg
      901 cgaaagccaa cctaaaagga actatttttt atgacctcgt ataataaaaa aatagtccgc
      961 acttcttcct atattgaaat ttgggaatat gaaaaaccaa tattttcaaa aggaaaatcc
     1021 attttagata ctgatactaa aagcaaggaa aaatcacaac gtagaacatt tgatgaactt
     1081 acacctgaag aacaagaaca acgtctggag cgtatgaaaa aaactcgctt agaagcaaag
     1141 tggaacttac ttcgattagt tgattgtaat tataatgata atacttcatt tctaaccctg
     1201 accactaaag aaaatattag cgacagaaac cacttcaaag acttactaaa aacttttata
     1261 aaacgcttta actaccacat ctttaatacc aagaaaagta aattaaaata tttaacagta
     1321 ttagaaaagc aaaaacgtgg agcatggcat gctcatattc tgcttttcag cgttccttat
     1381 gtaccacacc gtcaactctt ggaactttgg ggacatggag cagtacggat aaataaagtt
     1441 gatgtagaca gcaaagaaaa tcgcggtcga tatgtcacaa agtattttga aaaagggatt
     1501 ggacaagaat tgttggaaaa cttcggaaag caagcctatt tttcatcacg caacttaaaa
     1561 aagcctgatg aagatagatt ttatacttat gaagatttta attatgatag ttcagtcgta
     1621 ctatatgaaa cggaatacat tagtaaagtc tataaagacg gtcagtattt cgataatcat
     1681 gtaaagtata agaaaataaa actagatgaa taaaggagta agttacatga tagcaactgt
     1741 tactaagaat gacctagttg cccttggttt ttctgagggc acatctaaac atattatccg
     1801 ccaagggaaa gagctactga taacccgagg ctttacagtc tatcagaaca agcgaattgg
     1861 tactatacct gctactattg ctaccgaatt actcggtttt gatgttcaaa acagtaccct
     1921 caaggagtaa aatatggcaa ttagaaaagg taaagacggt aattgggtag ttgatattag
     1981 caacggtttt gacccaataa cactaaagca acgtagactt gtcagaaagg gatatatgac
     2041 gaaaaaagaa gctattgaag cagaacacta cttacgaagt gtagagttaa aagaacgctt
     2101 ctatggggct aaaataacta tctccatact atatgaacta cttaaggaag aagatagcat
     2161 aaatcatcga aaagcaagct atatcaatac acaagataat aattacaaca aacacatcaa
     2221 agattatttt tctatggtcg ataatgtcgg aaactaacct atgaagacat ctaccaattt
     2281 agagaacatc ttaggcaaaa agtagctaaa aattctgata aaccgttaag cacaaatact
     2341 attaacaaaa tcatgatttt actcaaaaaa atttttgatg ttggtttgcg aaaaggctat
     2401 tactcctcca atccagcaaa attgataaaa aagttgccaa ttgaaaaaac aaaaatgcaa
     2461 ttttggacag tcaaagaatt tcagaagttt ttgacactat ttgaacccga agaatacaat
     2521 atcaaactac tctttactgt tctattcttt acaggattaa gacttgggga agctcttgcc
     2581 ctaacatggc aagacatcga cttttcaagt aatacaattc atatcacgaa atctgtctat
     2641 gttaataaag gagtaactca tatcagcaca acaaaaacta aagcaggaac aagaagaatt
     2701 gttatcaata aaaaattaag ccaagaatta caagattggg aaaagcgaca aaaacatcta
     2761 ttagagcaat ttaccaacaa tagcctaaca ttacaagtgt ttcaaaatag tcctatcaca
     2821 attacgaaaa atgctattga aaagcagtac aagaaaatca ttgaacgtga tagcacacta
     2881 aaaaagatac gcatacacga ttttaggcac tcacacgctt ccctgctgat taaccaaggg
     2941 gaagattatc tagttgtaaa agagcgacta ggacatgctt ctataacaac cacgattgat
     3001 acctactctc acttgtaccc aagtaagcaa aaggatttag ctgataaact agatgattta
     3061 ctataaagta attgagtttt ataaatggaa agtttataat taaatcatgg atacatcaca
     3121 agtaataaaa gcacatgaag tagctaaaaa agcacacttt ggacaaattg atagagcagg
     3181 aattgactac attaaacacc ctgaaatagt agctagtttt gtcgcaactg atgaagagaa
     3241 agctgtcgcc tatctacatg atgtaattga agatacttca ctaaccctac tagacctaaa
     3301 aaaggaaggt ttttcaaaaa acattataga agccgtcgat atactcacaa agaaaaaagg
     3361 acaagattat caatcctatc tcaatctagt aaagaaaaat gaactcgcta gagttgtaaa
     3421 attggcggat ttacggcata attcagattt aacaagacta ccattaatta ctgaaaaaga
     3481 cttagagaga aataagaaat acagttctgc tattagattt ttaagagcaa agaaaaaaag
     3541 ttagtaacta aactataaaa ttttataaag agaaaataaa agacaaggct tataaacttt
     3601 gtctttatta ctatactggc ggtcggtctc gaactggcac atccttgcag atactacaat
     3661 tttattaaaa taaataacta taaaaagtga aatttt
//
LOCUS       DAVKWZ010000105         3653 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000105 DAVKWZ010000000
VERSION     DAVKWZ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.105"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            550..1089
                     /locus_tag="VW854_002047"
     CDS             550..1089
                     /locus_tag="VW854_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263218.1"
                     /translation="MNSKKIVPKTKTHTFDDVIEQGYCHRLSRYVPDAVVGGSYKYDS
                     TDALPYAKKLKNTLYGQNLSVKYVASLLDMWDRACQLFHTVTGTCLADDIFTSKKIYS
                     ELYFYNTNTSDFITDEIIELVKEKHRSYSRKADEDSILSVEHEFEINPDIYYYVLGKL
                     GWKRVKHNYLVKALAGALS"
     gene            1148..1378
                     /locus_tag="VW854_002048"
     CDS             1148..1378
                     /locus_tag="VW854_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263219.1"
                     /translation="MKNLYKYLFYLFFIGFGCTLYYVLLLKNVYSPIFEAIAILMGIT
                     MIIFLMIFIDMASPAGEERKHENVESKNLGDE"
     gene            1341..2702
                     /locus_tag="VW854_002049"
     CDS             1341..2702
                     /locus_tag="VW854_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEV0263220.1"
                     /translation="MRMLNQRISVMNKYPYFIRPRNIKIVRLAIQIFTLLLLMCSWIA
                     TIPFIARLVFSMFFISLLFLVERFFRSNFMANLRDKLNIREALFYMLISLNLYDEIDN
                     EVVDTAVLYFDVEDNKVIVCVPLFGNRYLKTLKNLEEYLCPTLGLSLLSKKEEIDKTI
                     YVLGHVVQIEQYVFNSNTLKREFFEDIPNPIIKLSNTQQFSLKSNTNIGIYGRTGTGK
                     TIALQWYLFNALAKGCGTNDNTYLGIVDGKAADLFALGKLLQEDLGEQIAVGSSPQML
                     AKLSREFVEIMNERFEIIKQNSSLNADAYDLGMTPNFLFVDELASIRDSCGSSKQGGK
                     ELWNEILQNLGLIARKGRQAGCHLCLSTQDPNAENIPVELRSQISAVLYLGGPGADRL
                     KMAFSMCELENVPTVSDRKGEALFYADGLNTVEPVLTIVPFVDLKTKQEFLQVVRNIL
                     PNY"
     gene            2800..3114
                     /locus_tag="VW854_002050"
     CDS             2800..3114
                     /locus_tag="VW854_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263221.1"
                     /translation="MKLNDFLKPELLGNRFFAVKGYTEVLDRETNKPVALRLNVSIQD
                     ENSDFFMEMIQVKINTLSPTASVQEMANNKTCPVSLKDLNVGQFNGNLWFACSDVLPV
                     TK"
     gene            3148..3537
                     /locus_tag="VW854_002051"
     CDS             3148..3537
                     /locus_tag="VW854_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006268423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263222.1"
                     /translation="MQTFNYDDFKNEKGIVSFSEAEQIYSSLLNSSNQLDKEFQEEWT
                     TFVLLCVEYASARGKWLTLSREEKLANDESRTVTHNKVIYQLKILKDLASEQGNDVAW
                     FEQFNDDRKRIGDFACYVAYIYALNAR"
BASE COUNT         1244 a          549 c          663 g         1197 t
ORIGIN      
        1 tttcctatta gtgacaatgt tttataaact tgtgtcattc ttctttctac tacaataaca
       61 aaattatttg ctttatctac tgttgtcata ttgttcctcc atctaaccat tatagcatcg
      121 caaatctttt ttgtctactt ttttaaaata aacggtctat ttaaaaatat tagacaaaaa
      181 gaccgtattt ttcttgacat agtggatacg atatgctaag atatcatcaa caggtaaata
      241 attacctcaa acaccgtgta gaaaggaaaa tttgtcatga ccccagaaga acaaatggca
      301 ttagagtatt tttatgaaga gatggacgcc tattattgtg aggaagaaca aggagagatt
      361 tttgattgat acgagaatta aatcatatct atagtttctc aggataattg gaaactatat
      421 ttgcaagata ctacatttat acacaaaaca caaaaaggag aagataaatg aatattgcaa
      481 gagcctcacc cagagtagag actaattgaa acaatgatac atatattaat tttgaaagga
      541 aatattgtta tgaattcaaa aaaaatagtt ccaaaaacta aaactcatac ctttgacgat
      601 gttatcgaac aagggtattg tcataggcta tcaagatatg ttcctgatgc agttgttgga
      661 ggttcctaca aatatgattc aacagatgca ttaccatatg cgaaaaaact taaaaataca
      721 ctatatgggc aaaatttatc tgttaagtat gtagcaagtt tgttggatat gtgggataga
      781 gcatgtcagt tatttcatac ggtaacaggg acatgcttag cggatgacat ctttacctca
      841 aagaaaattt atagtgaatt gtatttttac aatactaata cttctgattt tattacagat
      901 gagattattg aacttgttaa agaaaaacat cgttcatatt ctagaaaagc tgatgaagat
      961 agcattttat cagttgagca tgagtttgag attaaccctg acatctatta ttatgttcta
     1021 ggcaaattgg gttggaaaag agtaaaacac aattacctag ttaaggctct tgcaggggca
     1081 ttatcttagt ttaatctagg ggagtattcc tcccctttta tatcttaaaa aagaaaggaa
     1141 ttgcgtcatg aagaatctct ataaatatct tttttatttg ttctttattg gatttggttg
     1201 cacattatat tatgtgcttc ttttaaaaaa tgtctattct cctatctttg aagctattgc
     1261 aatattgatg ggtattacga tgattatctt tttgatgatt tttatagata tggcttcccc
     1321 tgctggagag gagagaaaac atgagaatgt tgaatcaaag aatctcggtg atgaataagt
     1381 atccgtactt tattcgtcct cgaaatatta aaattgttag actagctatt caaattttta
     1441 cccttttact tctcatgtgt tcatggattg ctacaatccc atttattgca cgtctagtct
     1501 tttccatgtt ttttataagt cttctttttt tagtagagag gttctttcgt tctaatttta
     1561 tggcaaattt aagagataaa ctcaatatca gagaagcatt gttttatatg ctaattagtt
     1621 taaatttgta tgatgaaata gataatgaag tagtagatac agcagttcta tattttgacg
     1681 ttgaggataa taaagttatt gtctgtgtcc cactatttgg gaatcgttat ctgaaaacct
     1741 tgaaaaattt agaggaatat ctatgcccta cgttgggctt gtccttgctg tctaaaaaag
     1801 aagaaataga caaaacaata tatgtgttag gtcacgtagt tcaaattgag caatacgtct
     1861 tcaatagtaa tacattaaaa agagaatttt ttgaagatat tcctaatccg ataattaaat
     1921 tatctaacac ccagcaattc agcttgaaat caaacaccaa cataggtata tatggacgaa
     1981 ctggaacagg caaaacaata gccttacaat ggtacttatt taatgcacta gctaaaggtt
     2041 gtggcactaa tgataatact tacctaggaa tagtggacgg taaggcagcg gatttatttg
     2101 cacttggaaa acttctccaa gaagacttag gggagcaaat agcagtcggc tcttcacctc
     2161 aaatgttagc aaaattatca agagaatttg ttgagattat gaatgaacgt tttgaaatta
     2221 tcaaacagaa tagttcatta aatgctgatg cttatgattt agggatgaca ccaaattttc
     2281 tatttgtaga tgaacttgct tccatccgcg atagctgtgg ttctagcaaa caggggggta
     2341 aagaactatg gaatgaaatt ttgcaaaact taggcttgat tgcacgtaaa gggcggcagg
     2401 caggttgtca cctctgcctt agtacccaag accctaatgc tgagaatatt ccagtcgaat
     2461 tgcgctccca aatatcggca gttttatacc ttggaggccc aggggctgat agattgaaga
     2521 tggcttttag catgtgcgaa ttagaaaacg ttcctacagt ttcagataga aaaggggagg
     2581 ctctctttta tgctgatggg cttaatacag tagagccagt gctaacaatt gtgccatttg
     2641 ttgatttaaa aactaaacaa gaatttttac aggtagttag aaatatactg ccaaactact
     2701 aataacggta tttttctctg ttggaatagg aattcttttc aacagagaaa aagagccaac
     2761 cgactgcatt gcaggaggta agaaagaaag gaatttatca tgaaattaaa tgatttttta
     2821 aaacctgagc tactaggcaa tcgcttcttt gccgtgaaag ggtatacaga agtattagat
     2881 agagagacta ataagcccgt agctttacga cttaatgtgt caattcaaga tgagaattct
     2941 gatttcttta tggagatgat acaagtcaaa ataaatacat tatcgcctac tgcttctgtt
     3001 caagagatgg cgaacaacaa aacttgtcct gttagtctta aagatttaaa cgtagggcaa
     3061 ttcaatggaa atttgtggtt cgcttgttct gatgttttac ctgtaacaaa atagctaaga
     3121 ttagataata tataaaggag ttttacaatg caaactttta actacgatga ttttaagaat
     3181 gaaaagggga ttgtttcatt ttctgaagca gaacaaatct attctagtct ccttaattcc
     3241 tccaatcaac ttgataaaga atttcaagaa gaatggacaa catttgtact tctatgcgta
     3301 gagtatgcct ctgcacgtgg taaatggctc acactctcaa gagaagagaa acttgccaat
     3361 gatgaatcta gaactgttac tcacaataaa gttatttatc aactaaagat tttaaaagat
     3421 ttagcaagtg aacagggcaa tgatgttgct tggtttgaac aatttaacga tgacagaaaa
     3481 cgtattggcg actttgcttg ctatgtagct tatatctatg ccttaaatgc tcgatagaca
     3541 attagaggat attatagcta tatattactc tatgtctgat attcatggaa attattaaat
     3601 aacatcttaa gaataattaa ttctatttat ctatcaccga aacaatgtgc tat
//
LOCUS       DAVKWZ010000106         3452 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000106 DAVKWZ010000000
VERSION     DAVKWZ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.106"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            156..2789
                     /gene="polA"
                     /locus_tag="VW854_002052"
     CDS             156..2789
                     /gene="polA"
                     /locus_tag="VW854_002052"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEV0263223.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELMDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVSESLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIMVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYVQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEIKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            2874..3311
                     /locus_tag="VW854_002053"
     CDS             2874..3311
                     /locus_tag="VW854_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEV0263224.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
BASE COUNT          940 a          608 c          877 g         1027 t
ORIGIN      
        1 gtgtactatt atagattcaa tatattatag cacattttta aatctaggtt tactaaattc
       61 tctggctcta tctatttatt cggaaaaagt ttgaaaaata cttgctctag ctcttcccaa
      121 tggtattttt tgattctttc ctttataatg ggtgtatgga taagaaaaaa ttattattga
      181 ttgatgggtc ttctgtagct tttcgggcgt tttttgcgct gtatcagcag ttggaccgtt
      241 ttaagaatgc ggctggtttg cataccaatg cgatttatgg ttttcagttg atgttgagtc
      301 atttgttgga gcgggttgag ccgagtcata ttttggtggc ttttgatgcg ggaaagacga
      361 ccttccggac agagatgtat gcggactata agggtggtcg ggccaagact cctgatgagt
      421 ttcgtgagca atttcctttc attcgtgagt tgatggatca tatggggatt cgtcactatg
      481 agctggctca gtatgaggcg gatgacatca ttgggacgct ggataagcta gcagagcagg
      541 atggttttga tattaccatt gtcagtgggg acaaggattt gattcagctg acggatgagc
      601 atacggtggt tgaaatttcc aagaaaggtg tggctgagtt tgaggccttt acgccagatt
      661 acctcatgga agaaatgggc ctcacaccag ctcagtttat cgatctcaag gcgctcatgg
      721 gtgataagtc ggataatatc cctggggtga ccaaagtcgg tgaaaagacg ggtattaagc
      781 tcttgctgga gcatggttcg cttgagggga tttatgaaaa tattgatgga atgaagactt
      841 ctaagatgaa ggaaaatctc atcaatgaca aggaacaggc ctttttgtct aaaacactag
      901 cgaccattga taccaaggca ccgattgcga ttggtttaga ggacttggtc tatagtggtc
      961 cagatgttga aaatcttggg aaattctacg atgagatggg cttcaaacag ctcaagcagg
     1021 ctttaaatgt gtcgtcagct gatgtgtctg agagtttgga ttttactatt gttgaccaaa
     1081 tcagtcaaga tatgctgagt gaagagtcta tcttccactt tgagcttttt ggtgagaatt
     1141 accatacgga taatttggtt ggatttgcct ggtcttgtgg ggataagctc tatgccacag
     1201 acaagcttga actgttgcaa gacccgattt tcaaggattt cttagaaaaa acatctctga
     1261 gagtttatga ctttaagaag gttaaagttc ttttgcaacg ttttggtgtg gatttgcagg
     1321 cgcctgcttt tgacatccgt ttggctaaat acctcctttc gactgtggag gacaatgaaa
     1381 ttgcgaccat cgctagtctt tatggtcaga cttacttggt tgatgatgaa actttctacg
     1441 gtaagggtgt taaaaaggcc attcctgaac gtgagaaatt cttggaacac ttagcttgta
     1501 aacttgctgt tttggtagaa acagagccta ttttacttga aaaactcagc gaaaatgggc
     1561 aattagagct tctttatgat atggagcaac ctctggcttt tgtccttgcc aagatggaaa
     1621 ttgctgggat tatggtcaag aaagagacct tgcttgagat gcaggctgaa aatgagcttg
     1681 tcattgaaaa actgactcaa gagatttacg agctggccgg tgaggagttt aatgtcaact
     1741 cgcctaagca gttgggcgtg cttctctttg agaaattggg acttcctcta gaatacacta
     1801 agaaaaccaa gacaggttat tcgacagcag tggatgtttt ggagcgtctc gctcctattg
     1861 ctccgattgt taagaaaatc ctggattacc gtcaaattgc taagattcaa tctacttatg
     1921 taattggctt gcaggactgg attttggctg atggaaagat tcatactcgc tatgtgcagg
     1981 atttgaccca gaccgggcgt ttgtctagtg tggatccaaa cttgcaaaat attcctgccc
     2041 gattggaaca agggcgcttg attcggaagg cttttgtgcc agagtgggag gatagtgtgc
     2101 tactcagctc tgattattca cagattgaat tgcgcgtttt ggcgcatatt tctaaggatg
     2161 agcacttgat taaggccttc caagaggggg cagatatcca tacttcgaca gccatgcggg
     2221 tctttggcat tgagcgtcct gataatgtga ctgcaaatga ccgtcgcaat gccaaggcag
     2281 ttaactttgg agtggtttat gggatttcag actttggctt gtctaataat ttgggaatta
     2341 gtcgtaagga agccaaagcc tacattgata cctactttga acgttttcca ggtattaaaa
     2401 actacatgga tgaagtggtg cgggaggcgc gtgataaggg ctatgtagag accctcttta
     2461 agcgtcgccg tgagttgcca gatatcaatt cgcgcaactt caatattcgt ggttttgcgg
     2521 agcgaactgc tatcaactca cctatccagg gttcggcagc agatattctc aagattgcca
     2581 tgattcagct ggataaagcc ttggttgcag gtggttatca gactaagatg ctgttacaag
     2641 tgcacgatga aatcgtcctt gaagtgccta aatctgaatt ggtagagata aaaaaattgg
     2701 tgaaacaaac catggaagaa gccattcaac tcagtgttcc tcttatcgca gatgagaatg
     2761 aaggggcaac ctggtacgag gctaaataaa aagggggcta gtcctccttt tttgtagtag
     2821 aattctgcaa accttttcaa aatgtgctat actgatgaaa aaggaggatt tctatgagtc
     2881 aagaatttat caatccaagt gatggcgtga ttcgtcagta tctcgcaacg agtaaaaccc
     2941 ttgctgtggt gggtttgtct gaccgtgaag aaacaaccag caatcgagta acaaaggaaa
     3001 tgcaggctcg gggctataaa atcatcccag tcaatcccaa ggcggcaggt ggcgaaatct
     3061 tgggtgaaaa ggcttatgct agcctcgctg aaattccttt tcctgtagat attgtcaatg
     3121 tttatcgtcg cagtgaattt ctgcccgatg tggcgcgtga ttttctcaaa gctgatgcca
     3181 agattttttg ggcacagcta ggacttgaaa gtttagaagc gaaagagatt ttgcgtgatg
     3241 gtggatgtga tgatatcgtg atgaatcgct gtatcaagag agaacatact cgcttgattg
     3301 aggaagcata agaaaaaggt agctgatggg ctaccttttg tgttatactc aatgaaaatc
     3361 aaagagcaaa ctagccgcgg gctgctcaaa acaccgtttt gaggttgtag atagaactga
     3421 cgaagtcagc tcaaaacacc gttttgaggt tg
//
LOCUS       DAVKWZ010000107         3230 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000107 DAVKWZ010000000
VERSION     DAVKWZ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.107"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            <1..1006
                     /locus_tag="VW854_002054"
     CDS             <1..1006
                     /locus_tag="VW854_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AlwI family type II restriction endonuclease"
                     /protein_id="HEV0263225.1"
                     /translation="KHNDLIKEYSDMCKRIFSLSGVISFNQGIVSLGRPWIFPKLFSL
                     LNGNFKLSGEASYEEYENDIKSSFYQDICISDILELSYTQVLEIQNQIAEEFGIADIS
                     NIKQFVADKQEREFREFVEQEFGITKVSEVLSFISQRNDKKVQELVTDNALVPTIFEY
                     ILAIAWYYISDKKFQLRKSMQLTFSADNLPLSHAGGNKGDIEIEYSDKMLLLEATLMD
                     KSTQKRGELEPVIRHSVNLALSTNKPLQTIFVANEVDDNVVNIFRATSFIQLNGTLTK
                     GSVKGLNIFALTIPEIINILDRKINEQRIFEKLDDFSTIEPYRIENGWREEIVSEILA
                     "
     gene            1074..>3230
                     /locus_tag="VW854_002055"
     CDS             1074..>3230
                     /locus_tag="VW854_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEV0263226.1"
                     /translation="MIQRGSEFRKWDLHIHSPYTVLNNQFDKLPDGSPDVEKFIQKIK
                     DEGISAVGLTNYFRFSDDDFKLKDRLNEEGIATFLNLEVRLSNINKSDELFDYHVVFG
                     NEVQDDIVKNLLGHLKANIGDDEKSFNRLSKTEIEDKAHIPFKDLLKVLNSDRELNGR
                     FLTGFLSRGHGSATSDSDSKNKAVYEDICINSDFLIHSSNEVNITKDREFWLDKSPHI
                     RPLLQSSDSHSLEQIGSKYSWVKADLTFDGLEQIKYEPEYRISVEKEKPTLKKDELVI
                     DKILYNGQDIYLSENLNTIIGGRSTGKSTLLNSIAKKLGRELNGDSYYFENMDDFQII
                     WKDQQEDNSRQIEYIPQEYMFALARDNKKLKDLVGSIIQSKEMNSELKKYEQNCATLH
                     LEIRGLLDSYKENQKNIHELIKPEAEKQATQNRIKTYETKKKELLNASEITEQERDLF
                     ENKNKELQLLKTQKEIHESDLRYVSSILPITFEVESLPTTTTPSQDLRVKIGQITARL
                     RDSVRAQQEHEIRIIKLEKESLIKAIENKISDIGNDDIYKKCVEAMKNNSEIARLNSL
                     IKNEVDILAKIEAFEKQRDEFDKVTEEIQKEIISKYKEYSNIRTELLNNFKIEDDNGD
                     NLKISVKFSLIDLEAEFDYINARGRSKQDFIEKMIGSFEEVVDSIFDEDSLAFNGNRD
                     KFSHIEHFFTTNFYEYSFEIEYQGDKFEQMSPGKKAF"
BASE COUNT         1222 a          389 c          600 g         1019 t
ORIGIN      
        1 taagcataat gatttgatta aagaatacag tgatatgtgt aaaagaatat tttctttgtc
       61 tggagtaatc agttttaatc aaggaattgt atcgcttggt cgaccttgga tttttcctaa
      121 acttttctct ttattaaatg gtaattttaa gctttctggg gaagcaagct atgaagagta
      181 tgagaacgat ataaagagtt ctttttatca agatatctgt atatcggata ttttagaatt
      241 atcttatacg caagttttag aaatacaaaa tcagattgcc gaagaattcg gcattgctga
      301 tatttcaaat attaaacaat ttgtagcaga caaacaggaa agagagttta gggaatttgt
      361 tgaacaagaa tttggtatca ctaaagtatc tgaagttttg agttttattt ctcaaagaaa
      421 tgataagaaa gttcaagaac ttgttacgga taatgcctta gttcctacta ttttcgaata
      481 tatattggcc attgcatggt attatatatc agataagaag tttcaactta gaaaatccat
      541 gcagttgaca ttttctgctg ataacctccc actatcacat gctggaggca ataaaggaga
      601 tatcgagatt gaatatagcg ataaaatgct tttacttgaa gcgacattaa tggataaatc
      661 aactcaaaaa cgtggtgaat tagagccagt tatacgtcat tctgtaaatt tagcattatc
      721 tactaataaa ccgttacaaa caatatttgt agctaatgaa gtagatgata atgtggttaa
      781 tatttttagg gctacatcgt ttattcagct aaatggaaca ttaacgaaag gtagcgtaaa
      841 agggctaaat atctttgctt tgacaattcc agaaattata aatattttgg atagaaagat
      901 taacgagcag agaattttcg aaaaattaga tgatttttct actatcgaac cttataggat
      961 agaaaatgga tggcgtgaag agatagtatc tgaaatttta gcatagcctg taggaaatgc
     1021 gagtaattat agaggtttgt ataagacaat tgatgtagaa aggtttgtat tcaatgattc
     1081 aaagaggttc agagtttaga aaatgggatt tacatattca ttctccctat acagtgttaa
     1141 ataatcagtt tgataagctg cctgatggaa gtccagatgt agaaaaattc attcaaaaga
     1201 taaaggacga aggtatttct gctgttggat tgaccaatta tttcagattt agtgatgatg
     1261 actttaagtt aaaagatagg ttaaatgaag aaggaattgc aactttttta aatcttgagg
     1321 ttagattatc aaacattaat aaatcagatg aactttttga ctatcatgta gtatttggaa
     1381 atgaagttca agatgatata gtaaaaaatt tacttggtca tttaaaggct aatattggtg
     1441 atgatgaaaa atcatttaat cgtttaagta aaactgagat agaagataag gctcatattc
     1501 cttttaaaga tttattaaaa gtcttaaata gcgatagaga acttaatggt cgttttctaa
     1561 ctggctttct ttcgagaggt cacggtagtg caactagtga ttctgattct aaaaataagg
     1621 cagtttatga agatatttgt atcaattctg attttttgat tcattctagt aatgaagtaa
     1681 atatcacaaa agatagagaa ttttggcttg ataaatcccc gcatataaga ccactacttc
     1741 aatcctcaga ttctcactct ttggagcaaa taggaagtaa atattcttgg gtaaaagcag
     1801 atttaacttt tgatggtcta gaacaaataa agtatgaacc agaatatcgc attagtgttg
     1861 agaaagaaaa accaactctc aaaaaggatg aacttgtcat tgataagatt ctatataacg
     1921 gtcaagatat ttatctatcc gaaaatctaa atacaattat tggtggtcgc tcaactggta
     1981 aatcaactct actaaattct atagctaaaa agctgggacg agagctgaat ggtgattcat
     2041 attattttga aaatatggat gattttcaaa ttatttggaa agaccagcaa gaagataatt
     2101 ccagacagat agagtatata cctcaagagt atatgtttgc tttagccaga gacaataaga
     2161 agcttaaaga tttggtagga agtattattc aatccaaaga aatgaactct gaattaaaga
     2221 aatatgagca aaattgcgct actctacatc ttgaaattag agggttactt gatagttata
     2281 aagagaacca aaaaaacatt catgagctaa ttaagccaga agcagaaaaa caagctactc
     2341 aaaatcgaat aaaaacatac gaaactaaga aaaaagaatt attaaatgct agtgagataa
     2401 cggagcaaga aagagattta tttgaaaata aaaacaaaga attgcagtta ttgaaaacac
     2461 aaaaagagat tcatgaatct gacttgcgat atgtatcaag tattcttcca attacatttg
     2521 aggttgaaag tttgcccaca acgactactc cgtctcaaga tttaagggtt aaaattggtc
     2581 agattacagc tcggttgcgt gattccgtca gagcacaaca ggaacatgaa attaggataa
     2641 ttaagttaga aaaagaaagt ctaatcaaag ctattgaaaa taaaatttcg gacataggaa
     2701 atgatgacat atacaagaaa tgtgttgagg caatgaaaaa taattcagag atagcgaggt
     2761 taaattcttt aattaagaat gaagttgata ttttagctaa aattgaagcg tttgaaaaac
     2821 aaagggatga attcgataaa gtaacagaag aaattcaaaa agagattatt tcgaaatata
     2881 aggaatactc taatattaga actgaattat tgaataattt caagattgaa gatgataatg
     2941 gagataatct aaagatttca gtcaagttct cattaattga tttggaggct gaatttgatt
     3001 atattaatgc tcgaggtcgt tcgaaacaag attttataga aaaaatgata ggttcttttg
     3061 aagaagttgt tgattcaata tttgacgagg atagtcttgc gtttaatgga aatagggata
     3121 aattcagtca cattgagcat ttcttcacta ctaattttta cgaatatagt tttgagattg
     3181 aatatcaagg tgataaattt gaacaaatgt ctcctggtaa aaaagcgttt
//
LOCUS       DAVKWZ010000108         3059 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000108 DAVKWZ010000000
VERSION     DAVKWZ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.108"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            245..1531
                     /locus_tag="VW854_002056"
     CDS             245..1531
                     /locus_tag="VW854_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV0263227.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESPEKFEAIKQDLVDEMWKVAQRELATGFYYGIPSENEQLFGARRKIPEYKFVAEVV
                     SYDDAVQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQSGDMVRALKEGLINFYKEDGTSVTVRA"
     gene            1546..1857
                     /locus_tag="VW854_002057"
     CDS             1546..1857
                     /locus_tag="VW854_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263228.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCLFCFSRFNPGCQNHKERYF
                     K"
     gene            2117..2845
                     /locus_tag="VW854_002058"
                     /pseudo
     CDS             2117..2845
                     /locus_tag="VW854_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
BASE COUNT          947 a          533 c          665 g          914 t
ORIGIN      
        1 agtcatctaa ggactaattt atgtattttt acgagtcagt atcttgggat actggttttt
       61 acttttctag actttttgac tacttgttaa aactgggata attttcgact gtttaacagt
      121 tattatgcaa agtctaaaag attagaattg tcaaaacaat ccgtctaggc ttgattttat
      181 cctttattta ctataaaatg agaaggaaaa atgtcaaact tttatattgc aaataggaga
      241 aatcatgaca aaaacattaa aacgtcctga ggttttatca cctgcaggga ctttagagaa
      301 gctaaaggta gctgttcagt atggagcaga tgctgttttt atcggtggtc aggcctatgg
      361 tcttcgtagc cgtgcgggaa actttacttt cgaacagatg gaagaaggcg tgcagtttgc
      421 ggccaagtat ggtgccaagg tctatgtagc ggctaatatg gttatgcacg aaggaaatga
      481 agctggtgct ggtgagtggt tccgtaaact gcgtgatatc ggaattgcag cagttatcgt
      541 atctgaccca gccttgatta tgattgcagc gactgaagca ccaggccttg aaatccacct
      601 ttctacccaa gccagtgcca ctaactatga aacccttgag ttctggaaag agctaggctt
      661 gactcgtgtc gttttagcgc gtgaggtttc aatggaagaa ttagctgaga tccgcaaacg
      721 tacagatgtt gaaattgaag cctttgtcca tggagctatg tgtatttcat actctggacg
      781 ttgtactctt tcaaaccaca tgagtatgcg tgatgccaac cgtggtggat gttctcagtc
      841 atgccgttgg aaatacgacc tttacgatat gccatttggg aaagaacgta agagtttgca
      901 gggtgagatt ccagaagaat tttcaatgtc agccgttgac atgtctatga ttgaccacat
      961 tccagatatg attgaaaatg gtgtggacag tctaaaaatc gaaggacgta tgaagtctat
     1021 tcactacgta tcaacagtaa ccaactgcta caaggcggct gtggatgcct atcttgaaag
     1081 tcctgaaaag tttgaagcta tcaaacaaga cttggtggac gagatgtgga aggttgccca
     1141 acgtgaattg gcaacaggtt tctactacgg tataccatct gaaaatgagc agttgtttgg
     1201 tgctcgccgt aaaattcctg agtacaagtt tgtcgctgaa gtggtttctt atgatgatgc
     1261 ggtacaaaca gcaaccattc gtcaacgaaa cgtcattaac gaaggggacc aagttgagtt
     1321 ttatggtcca ggtttccgtc attttgaaac ctatattgaa gatttgcatg atgccaaagg
     1381 caataaaatc gaccgcgctc caaatccaat ggaactattg actattaaag tcccacaacc
     1441 cgttcaatca ggagacatgg ttcgtgcatt aaaagaagga ctcatcaatt tttataagga
     1501 agatggaacc agcgtcacag ttcgagctta agaaaggaaa aggaaatgat agaggcacag
     1561 ggtttcttag tggataagca aacaagatgc attcattacc atagcaagct ggatattatt
     1621 gctttacaat gctatgattg taaaaagtat tatgcttgtt atcggtgtca tgattcatta
     1681 gaaaatcacc cttttgagcc ttatccctta tctttgatac aggataagcc tattttatgt
     1741 ggtgtttgtc taaaactatt aacatataag caatataaag aaagcttaag ttgcctcttt
     1801 tgtttttctc gctttaatcc aggttgccaa aatcataagg aacgctattt taaatagcaa
     1861 atcatctagt tttgaagtag gagaaaactc aattccaaga gaaaatgaag taaatcttcc
     1921 cacaataaaa cgcataatat caagattgtt caatacctga tactatgcgt ttttaagatt
     1981 ttaaagactt ttttcctagc ccctttttac tttatctggt attttgacta cttgttaaaa
     2041 ctggggtaat tttcgactgt ttaacagtta ttatgcaaag tctaaaaggt tagaattgtc
     2101 aaaacaatcc gtctagagta tgcgtgatgc caaccgtggt ggatgttctc agtcatgccg
     2161 ttggaagtac gacctttacg atatgccatt tgggaaagaa cgtaagagtt tgcagggtga
     2221 gattccagaa gaattttcaa tgtcagctgt tgatatgtct atgattgacc atatctcaga
     2281 tatgattgaa aatggtgtgg acagtctaaa aatcgaagga cgtatggagt ctattcacta
     2341 tgtattaaca gtaaccaact gctacaaggc ggctgtggat gcctatcttg aaagtcctga
     2401 aaagtttgaa gctatcaaac aagacttggt ggacgagatg tggaaggttg cccaacgtga
     2461 actggctaca ggattttact atggtacacc atctgaaaat gagcagttgt ttggtgctcg
     2521 tcgtaaaatc cctgagtaca agtttgtcgc tgaagtggtt tcttatgatg atgcggcaca
     2581 aacagcaact attcgtcaac gaaacgtcat taacgaaggg gaccaagttg agttttatgg
     2641 tccaggtttc cgtcattttg aaacctatat tgaagatttg catgatgcca aaggcaataa
     2701 aatcgaccgc gctccaaatc caatggaact attgactatt aaggtgcctc aacctgttca
     2761 agctggagac atggttcgtg cattaaaaga aggactcatc aatctttata aggaagatgg
     2821 aaccagcgtc acagttcgtg cttaatgtag ttgtttagtt ttaaaaaatt atgcaaagct
     2881 ccatatacaa cacttaaacg agattaaaga atggcgaaat cccttgatgc gcaagagatt
     2941 agctgtcctt ttttattttt taagtgataa agtcggagct taggtatcaa agcctatcaa
     3001 attaaacaaa gaagtgatgt cttagatatt ttgaaaaaaa ttaataagca gaaaactct
//
LOCUS       DAVKWZ010000109         3043 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000109 DAVKWZ010000000
VERSION     DAVKWZ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.109"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(232..765)
                     /locus_tag="VW854_002059"
     CDS             complement(232..765)
                     /locus_tag="VW854_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEV0263229.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(854..1630)
                     /gene="recX"
                     /locus_tag="VW854_002060"
     CDS             complement(854..1630)
                     /gene="recX"
                     /locus_tag="VW854_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEV0263230.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQNFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINTNQLSGDKGPYVLTQKLAQKGISKSTIEENLKEFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            1668..3023
                     /gene="rlmD"
                     /locus_tag="VW854_002061"
     CDS             1668..3023
                     /gene="rlmD"
                     /locus_tag="VW854_002061"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV0263231.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
BASE COUNT          969 a          540 c          549 g          985 t
ORIGIN      
        1 attttttgta ttaaacgata taagtttgtt gtaaacttac taggaataaa gacattaaaa
       61 aataatagta tatctatttg ttttatatat tttacgaatt ctgcataaat ctctttctag
      121 taatgtgttg taactctgct ataatggatt tattcctttt tgtgtttaca caatttattt
      181 tatactacca aaaaaacaag gtcaggattt tgttcctgac ctttgacaac tttaccgatt
      241 ctttagttct acatagcgct tgtaccaaat gtttacatag gcttctgaga aaggaccacg
      301 tccattgtta atccaatcaa caagaatttt gacatgttct tttaaaatat agtccaagtc
      361 atcagaataa ttcattttgc gtttgtgacg ctcgtactct tcaacgtcca agagacgttt
      421 ttccccatct gtaaaaattt taacatccaa atcgtaatca atatacttca gtgcttcttc
      481 atccagatag taggggctag ccatattgca atagtaagaa gttccattat cacgaatcat
      541 ggcaatgata ttaaaccaat atttcttgtg aaagtaaaca atagccggtt ctcgagtgac
      601 ccaacgacga ccatcacttt cggtaacaag tgtatgatcg ttgacaccaa taatggcgtt
      661 ttctgttgtt tttagtacca tggtgtcccg ccaagttcgg tggagactcc catcatgctt
      721 ataactttga attgtaataa agtcgccttc ttttggaagc ttcataacta accaactttc
      781 tacaatttat aagtttatca tttactattg taccataaaa ttacctaaaa tctgtgaatt
      841 tcacttggaa atattaaaga tattctctaa gagcgcttgc tatatccgaa aaatcgtagc
      901 cctttcgtgc taaaacttga gttaaacgct gcttcagttc gtatccttca tactttcgag
      961 catacttagt atattgctta tcaagttcct tgaagatgag ttcttgagtc gtttcttggt
     1021 caacttggct atctaagtcg tcaaaggcaa ttttagcatc agaataagag aagcccttgt
     1081 tagtcaagtt ctggataatc ttatcttgca gggcacgagc tggaagtttt ccctcatatt
     1141 ttttcaatag tttattagct acacgttgag caacttcaga aaaatcaaat tctttcaagt
     1201 tctcttctat agtagatttt gaaatccctt tttgagctag tttctgagtc agcacataag
     1261 gacccttgtc tcctgaaagt tgattggtat tgatgatagc ataagcgtac tgaccatcat
     1321 taatccactt atcttcttta agattagcaa taacttgaga aacgatgttt ttatcaatat
     1381 cgtatttttt cagatattct ctgacttctt tttcagtgcg tgctttaaag gataagtggt
     1441 agagggccag attcttacca taagaaaatt gagcaaagtt ctgaatctct ttcaattcct
     1501 cttcgcttat caccttatct ctcgataaca taaaacgaac aattgtatct tcggtgatat
     1561 agcatttgtc gccattatca agctccatca gatagagtct ttttttcttt tcaagttttg
     1621 tgattttcat agctctatta taactcaaaa tgtgataaga taggggtatg aatctgaaag
     1681 tgaaacaaaa aataccatta aaaatcaagc gcatgggaat taacggtgag ggaatcggct
     1741 tttaccaaaa aacattagtc tttgtaccag gagctctcaa aggcgaagat atctattgtc
     1801 agattacttc tattagacgc aactttgttg aagcaaaatt actgaaggtc aacaagaagt
     1861 ctaaatttcg aattgtgcca tcttgtacta tttataatga atgcggaggc tgccaaatca
     1921 tgcacctgca ttatgataag cagctggagt tcaagacgga cttacttcat caagcgctga
     1981 aaaaatttgc tcctgcagga tatgaaaatt atgaaattcg tccaactatt ggaatgcagg
     2041 aaccaaaata ttacagagct aagttacaat ttcagactcg aaaatttaaa aatcaggtca
     2101 aggcgggctt atatgcacaa aactctcact atttagtaga gttgaaagac tgcctggtac
     2161 aagataagga aacccaagtg attgctaatc gcttagcaga attacttact tatcaccaga
     2221 ttccaatcac ggatgagaga aaagttctag gtgtccgtac tattatggtc cgacgcgcga
     2281 gaaagaccgg acaggttcag attattattg ttacaaaccg ccagcttaat ttaactcaat
     2341 tggtaaaaga gttggttaaa gatttcccag aagttgtgac agtagctgtt aatacaaata
     2401 cagctaaaac cagtgagata tatggtgaaa agacagagat tatctggggg caagagagta
     2461 ttcaagaagg tgtactcaat tatgaatttt cactatcccc tcgagctttt tatcaactaa
     2521 atcctgagca aacagaagtc ctctatagcg aagcagtaaa agcgctggat gttgataaag
     2581 aagaccattt gattgacgct tattgtggag ttggaacgat tggatttgcc tttgcaaaga
     2641 aagtaaaaac actcagaggt atggatatta ttccagaagc tattgaagat gccaagcgaa
     2701 atgctaaaag aatgggattt gacaatactc attatgaagc tggaacggca gaagagatta
     2761 ttcctcgttg gtacaaggaa ggctaccgag cagatgcttt gattgttgac ccaccacgta
     2821 caggtctgga tgataagtta ttagatacta ttcttactta tgtaccagaa aaaatggttt
     2881 atatttcttg taatgtttcg accttggctc gtgatttggt acgcttagta gaagtctatg
     2941 atcttcatta tatccagtcg gtcgatatgt tcccacatac agctcgaact gaagctgttg
     3001 taaaattaat aaaaaaagtt taaaaaaggt gttgacaaaa cat
//
LOCUS       DAVKWZ010000110         2926 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000110 DAVKWZ010000000
VERSION     DAVKWZ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.110"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(370..762)
                     /locus_tag="VW854_002062"
     CDS             complement(370..762)
                     /locus_tag="VW854_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV0263232.1"
                     /translation="MELDKFKTMMNVRKRMTYFLRFQRMAGSENQVRIDEEAWKLVLP
                     DQWHLSGEHEKAIREGLEIFAHDINSIENERARKYFIIHYCYMRKKTMSECVEMAGTS
                     STSYHRYKQISVLNFARIHQNGELEAYK"
     gene            complement(799..1056)
                     /locus_tag="VW854_002063"
     CDS             complement(799..1056)
                     /locus_tag="VW854_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263233.1"
                     /translation="MKKARLDSEILEELLEKNKVNYLDANQYAIEIGEILAKVLGAYL
                     SNINQEDVERRKQIGIESAEERKRKRDERIAKFGNPRRRLS"
     gene            complement(1065..2363)
                     /locus_tag="VW854_002064"
     CDS             complement(1065..2363)
                     /locus_tag="VW854_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000172765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263234.1"
                     /translation="MTLKGMGYLRKKLANYKMGVDTRYNQYAMQHNDIDVGITIPPQI
                     RQQYRAVLGWTAKGVDSLADRLVFREFANDEFGANEIFAQNNPDVFFDSAILSALIGS
                     CCFVYISQGDDDDAPRLQVIEASNATGVLDPITGLLIEGYAVLKRDDNGYAVLEAYFT
                     SDVTWFYPKDGKPFAIENPTGVPLLVPVIHRPDTVRPFGRSRITKAGMYYQRYAKRTL
                     MRSDVTAEFYSFPQKYVLGLSQDAEAIDTWKATVSSLLTFTKDDEGEKPNVGQFTTSS
                     MSPFTEQLRTAAAGFSGEMGLTLDDLGFVSDNPSSVEAIKASHENLRLAGRKAQRSLG
                     SGLLNVAYVATCLRDEFPYLRKQFNKTVVKWEPLFEADANMLTLIGDGVIKLNQAVPG
                     YMDAETIRDLTGIKGSDKPAPVVKEGADGGRYVSESTPEN"
     gene            complement(2561..2905)
                     /locus_tag="VW854_002065"
     CDS             complement(2561..2905)
                     /locus_tag="VW854_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001128258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263235.1"
                     /translation="MPLPTGKIDLNEYGKHLAEKSAEIYKEKTKQAVDAMERHRQECL
                     ARDAKWQAVIDRSDELLKEKKEREHQQEIERLMEEKRKEVDREVSQRLSMPNEHQKKL
                     NKAMSEMLKDLF"
BASE COUNT          795 a          734 c          491 g          906 t
ORIGIN      
        1 tcaaaataat ttgacttagg catcatttca cacctcaata tagcgcctaa acccttgata
       61 ttactgactt tttgaaaaat atcatttgat tagaaatcat taaaaattct caaaaagtcc
      121 acaaaaaaaa agccccacaa aagggcaata aatgacgagt tcagcaggca agaaactagc
      181 acggtcaaac gtgctttttt attgctgatt gctgataggt atattatacc atgaagcgcg
      241 tagtttataa gattatgacc tcacaaaagc cccccagatt cgtttctaag ggcttctagt
      301 ttatctatgg tgttttagtt gtccagtacg aaacaaagcg aaataagggg gcgaatatgc
      361 agcctcagcc tacttatatg cttctagctc tccgttctgg tggattcttg caaagtttaa
      421 gacggatatc tgtttgtatc ggtggtagct agtggagcta gtacctgcca tttctacgca
      481 ttcgctcata gttttctttc tcatatagca ataatggata atgaagtatt ttctggctct
      541 ctcgttctct atgctgttga tgtcgtgggc gaatatctcc aacccctcac ggattgcttt
      601 ttcatgctca ccgctcaaat gccactgatc aggtaagaca agtttccaag cctcttcatc
      661 tatcctaact tggttttcac tacctgccat cctctgaaat ctcagaaagt aagtcatccg
      721 ctttctgacg ttcatcatcg ttttaaactt atccagctcc atttttaacg acaacctttc
      781 tactcctgta acttatgcct agcttaatct tcttctagga tttccaaact tggcaatcct
      841 ttcatctcgt tttcttttac gttcttcagc agactctata cctatctgtt ttcgacgctc
      901 cacatcttct tgattgatat tactcaagta agctcctaag acctttgcga gtatttcacc
      961 aatttctata gcatattgat tggcgtccag ataattaact ttattctttt ctagtaactc
     1021 ttccagaatc tcactatcaa gcctagcttt tttcaaattc gtccttaatt ttctggagta
     1081 gactcggaaa catatcttcc accatctgca ccctccttca ctactggagc aggcttgtct
     1141 gaccccttaa ttccagtcaa gtcacggatg gtttcagcat ccatatagcc aggcaccgct
     1201 tgattcagtt tgataacacc atcaccaatc aaggtcagca tgttagcgtc cgcctcaaat
     1261 aaaggctccc acttcacgac cgttttattg aactgtttcc tcaaataagg aaactcatca
     1321 cgtaaacaag tagcgacata agccacattc agcagaccag aacccagaga gcgctgagcc
     1381 ttccgaccag ctaaacgcaa gttctcatga ctagccttga tggcttcaac ggatgacgga
     1441 ttgtcagaaa caaaaccaag atcatccaag gtcaatccca tttccccaga aaatccagct
     1501 gctgcagtcc gtaactgctc agtaaaagga gacatgctgg acgtggtgaa ttgtcccaca
     1561 ttcggctttt ccccctcatc atctttcgta aacgtcagca agctagatac agttgctttc
     1621 caagtatcaa tcgcctcagc atcttgactc aatcccaaca catacttctg agggaatgaa
     1681 tagaactcag cagtaacatc tgaccgcata agcgttcgtt tagcatatct ctgatagtac
     1741 atcccagcct tagtaattcg tgaccgacca aacggccgaa cagtatcagg cctatgaatg
     1801 actggcacca gcaaaggaac acccgtcgga ttttcgattg caaacggctt accatctttc
     1861 ggatagaacc aagtcacatc actagtaaaa taagcctcaa gcacggcata accattgtca
     1921 tcccttttca aaacggcata gccctctatc aacaagccag tgataggatc cagaacacca
     1981 gttgcattac tcgcctcgat aacctgcaac cgaggagcgt catcatcgtc cccttgcgag
     2041 atgtaaacaa aacaacacga cccaatcaga gccgaaagaa tcgcgctatc aaagaataca
     2101 tctggattgt tctgagcaaa gatttcattc gctccaaact catcattggc aaactcacga
     2161 aagaccaaac gatctgcaag actgtcaaca cccttagcag tccaacctaa gaccgctcga
     2221 tattgttgcc tgatttgagg tggtatcgta ataccaacat ctatgtcatt gtgttgcata
     2281 gcatactgat tgtatctagt atctacaccc attttataat tggctagctt cttcctgaga
     2341 tagcccatac ctttcaatgt cattttatac aactaccttt catttcccgc gagaaaaaat
     2401 gtacagtgat ggtgggaagt tcgggagctc cgagggggag gggggtatcc cccctccttc
     2461 tcccttgctt ctccaatctc tttcacaaaa aatatttttc cgattcactt tattaaaaaa
     2521 ctttttaatt atttttttat ggagtctttt tatgttctct ttaaaataaa tctttcaaca
     2581 tttcactcat cgctttgttc agttttttct ggtgttcatt tggcattgat aggcgctgac
     2641 taacttctct atcaacctct ttgcgcttct cttccatcaa gcgttcaatc tcttgctggt
     2701 gctctctttc cttcttttct tttaatagtt catcggaacg atctatgaca gcttgccact
     2761 tggcgtctct tgctaaacat tcttgtctat gtcgctccat cgcgtcaact gcttgtttag
     2821 ttttctcttt gtaaatctca gctgactttt ctgctaaatg cttcccgtat tcgtttaaat
     2881 caatcttacc agtaggtagg ggcattctat atacctcatc tttcta
//
LOCUS       DAVKWZ010000111         2844 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000111 DAVKWZ010000000
VERSION     DAVKWZ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.111"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            <1..257
                     /locus_tag="VW854_002066"
     CDS             <1..257
                     /locus_tag="VW854_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263236.1"
                     /translation="GFLGIIYDNEALRDLSKQYLNKNISELQDGSFEDGIQDAVASKQ
                     IVVINEFIACLNSRCVPSQYDRFFYKDRPYIFSRKSPIKG"
     gene            571..1548
                     /locus_tag="VW854_002067"
     CDS             571..1548
                     /locus_tag="VW854_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263237.1"
                     /translation="MKNKRYFFDTILIILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFYFIFVIIIGARKYYRILFHQLMGFS
                     PGIAVFYKESQTTKNLFKFYYFLYFTTLISYYFFFTFVYDKSLLLPLIPFSIIIALVQ
                     KLYRIENQQLFLLKSKVLTILESKRDCEFNLQDYHEIWKLQSKSELPCVALSYISLIK
                     PYLSESVREQIDLLEVKRFKKINHPISLYGMLDVIKLNLYLRHYNEKNKYESMLKKIL
                     EVRPDFVLIEQNIDDSLNSSQPLSLSLAISEIQLLLEVYMGIKHVSIRR"
     gene            complement(2095..2253)
                     /locus_tag="VW854_002068"
     CDS             complement(2095..2253)
                     /locus_tag="VW854_002068"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263238.1"
                     /translation="MAQIVVIASPIDIQAANPLIADSANPATLFATRTPTYLAAAPAI
                     ALVTRRKP"
     gene            2507..>2844
                     /locus_tag="VW854_002069"
     CDS             2507..>2844
                     /locus_tag="VW854_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263239.1"
                     /translation="MIRKLIIFLLMLPLFVLWLELHVLVNNLQLNLEIPFDFVVSTSL
                     TFFVLILSKIVLDILYALKDLYKKEALITIFPFIFIRRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWS"
BASE COUNT          947 a          366 c          503 g         1028 t
ORIGIN      
        1 ttggattttt gggaatcatt tacgataatg aagcattaag agatttgtca aaacaatatt
       61 taaacaaaaa tatttcggaa ttacaagatg gttctttcga ggacggtata caagatgctg
      121 ttgcctctaa acaaatagta gttataaatg aatttatagc ttgtttaaat tccagatgcg
      181 tgccatccca atatgataga tttttttata aagatagacc ttatattttt tccagaaaga
      241 gtcctattaa ggggtgaaaa tttgttacat ttggattaac aagtgtccta gggattagaa
      301 ataactctag tatagctaga tatttttgag taactagaaa tatcattagt ctacccatat
      361 gattgccaga aaagatggct gatgaatctt atttatactg ttgcagtagg cgttgtctag
      421 gataaataag ctatcttgat gggggagatt ggaataattt tagtagaaac attaaaggat
      481 tatggacaag tagcgcagga ttaggtgatg caatgtgcta agttggcttg gtgttagcag
      541 tagtattatt taacaatgga aagtagacta atgaaaaata agcgttattt ttttgacaca
      601 attttaataa ttttactgct tataagcacg atattttgtg tcagcccagt atttataaaa
      661 ttggacatct taggaactcc ctctcatgcc atcttaacct ttgtattggc gataccgtta
      721 ttctatattt tgtctcaatg cttacatact ttgctattac tagtttcctc aatcttttgt
      781 aaattaagac caatttattt ttattttata tttgtgatca ttataggagc taggaaatac
      841 tatcgaattc tctttcacca actaatggga tttagtccag gaatagcagt gttttataag
      901 gagagtcaaa caactaagaa tctgtttaaa ttttactact tcttgtactt tacaacacta
      961 atttcatact attttttctt tacttttgtg tatgacaaat cgttgctttt gcctcttatt
     1021 ccattttcta ttatcatagc attagtacaa aaactttata gaattgagaa tcaacaatta
     1081 tttctgttaa aatcaaaagt actaacaata ctggaatcta agagggattg tgagtttaat
     1141 cttcaggatt atcatgaaat ttggaaattg caatcaaaat ctgagttgcc atgcgtggct
     1201 ttatcttata tcagtttgat caagccatat ctttcagaga gtgtgcgtga acaaattgat
     1261 ttattagaag ttaagcgatt taaaaaaata aatcatccaa tatcacttta tggtatgttg
     1321 gatgttataa aattaaatct atatttacgg cattataatg aaaaaaataa atatgaatca
     1381 atgttaaaga aaattttaga ggtaagacca gactttgttt taatcgagca gaatattgat
     1441 gatagtttaa atagctcgca acccttatct ttatctttag ccatttcaga aatccaactt
     1501 ttgcttgaag tgtatatggg gatcaaacat gtttcaatta ggagataaaa ccagaataat
     1561 atttgccatt ataggggcaa gttgcatatt agtcagtatg tatcttattc cacttattaa
     1621 aactgagaat aaattttggg aaatactttt atttatatta ggtgtattgc taattggaat
     1681 tgcgttacta agtcaaaaaa aataaatttt gaaatagtat tagtgagttc gcaatcattt
     1741 attgctagtg gtttagaact gttaagtttc aaacaataat tgattttaaa ccttaggtaa
     1801 cctataaaaa caaataaggt agagaaagga tatcttatat aggtagctaa gtgataaaaa
     1861 cacagtcgtc tatcacttgt taggaaacta tcctaactat gcttaataaa aaaggagaaa
     1921 ataaatggaa ctcgtattac caaataatta tgttgctctt gagcaagaag agatgatgta
     1981 tcttgatggg ggattttcta ttctgagatg acctgttgca acagccatta atatagcttt
     2041 taatggtgtt ttaggtggag gagcaatcag tctagttaga aattatattc gtaattatgg
     2101 tttgcggcga gttacaagcg caattgctgg agcagctgca agatatgttg gggtacgagt
     2161 tgcaaataga gtggcaggat ttgcactgtc tgctattaat ggatttgcag cttggatgtc
     2221 aattggcgat gctattacaa caatctgggc caacaatgat gtaaatagga gagacccaaa
     2281 tttaaacgcc ttgtggtaac tagatatggg tattaaaatg gaaaaaattt ttgttattat
     2341 tttttttgta tgtttattta tatcatccat aactttttta gcctatgatt ttgttagcga
     2401 agaaataaaa aagttgatta tttggatgaa tgtcgttttt ttgattttaa tcatagcaat
     2461 gataatttat ccaaaatcaa gaaaatgata aggacactgt aaaattatga tacgtaaact
     2521 aatcattttt ttattaatgc tccctctttt tgtcttgtgg ctagagttgc atgtcctagt
     2581 gaataacttg caattgaatc tagaaattcc ctttgatttt gtggttagta cgagtcttac
     2641 tttttttgtt ttgatattat caaaaatagt tttagacata ctatacgcat tgaaagattt
     2701 gtacaagaaa gaagctctta ttacgatttt tccctttatt tttataagaa ggaaaaaagt
     2761 gaatgtgaga ttctcgccct atttttcatt ccatcgtaaa agtttgtcgc ctgatgattt
     2821 aagatctagg attatatgga gttt
//
LOCUS       DAVKWZ010000112         2721 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000112 DAVKWZ010000000
VERSION     DAVKWZ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.112"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(<1..292)
                     /locus_tag="VW854_002070"
     CDS             complement(<1..292)
                     /locus_tag="VW854_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263240.1"
                     /translation="MSEVDFNEAVNYEFTSDTCQLANSIYQSLFKFFDKKNFSGDLIF
                     TWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLNMNRNGCMGDF"
     gene            complement(350..1063)
                     /locus_tag="VW854_002071"
     CDS             complement(350..1063)
                     /locus_tag="VW854_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEV0263241.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFSHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYDLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            complement(1521..1901)
                     /locus_tag="VW854_002072"
                     /pseudo
     CDS             complement(1521..1901)
                     /locus_tag="VW854_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443785.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
     gene            complement(2323..>2721)
                     /locus_tag="VW854_002073"
     CDS             complement(2323..>2721)
                     /locus_tag="VW854_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEV0263242.1"
                     /translation="VQRNKNGQADTNQTEKPNEEKPQTEKPEEETPREEKPQSEKPES
                     PKPTEEPEEESPEESPEESEEPQVETEKVKEKLREAEDLLGKIQNPIIKSNAKETLTG
                     LKNNLLFGTQDNNTIMAEAEKLLALLKESK"
BASE COUNT          826 a          535 c          431 g          929 t
ORIGIN      
        1 gaaaatctcc catacagcca ttacgattca tattgaggct atattttcga ttagtaagat
       61 aattcggaaa tatatttcct attactctaa gattatctac ttgtgaatcc cttctcccaa
      121 tataatcccc ttctttgact aatgatggag atttccaagt aaaaattaaa tcgccagaga
      181 aatttttctt atcaaaaaac ttaaaaagag attgataaat actatttgct aattgacaag
      241 tgtcagatgt aaattcataa ttaacagctt cattaaaatc aacttcagac atttcaatgt
      301 actcttcaat agtaatatta gcttttttgt agtcattagc tcgtctcctt taaaatgtga
      361 tcttttcaag aatattttta tctaaacaat ccagcaagtc ttggtaagaa tagacttcgt
      421 aagtcggctg ggcttgtgtg tgattttcga ggtgatgagg attataccag atagtgtcaa
      481 tccccgcatt attgccacct tgaatgtcgg cggttagaga atctccaatc atcagcgtct
      541 tttctttact aaatccagca atttgctggc caatcttttc ataaaaaaga gcatccggct
      601 tttgagtttg caactgttct gagataaaga cttgattgaa ataaggtgct agaccagatt
      661 gagccaaacg tcctgtctga atggcagtaa tgccatttgt cgcagcatac aagtcataat
      721 cacgctcaat gaggctgtcc aagagatcat gagcgcccga tagtgtttgt ccctgctgag
      781 cgagataaaa ttggtaacgc tgggcaagaa aactaccgtc tttttcctgt ccaaaatgag
      841 aaaataaacg agaaaagcgc gtgttaacca gctcttgttt actgattttc ttcagctcca
      901 agtctttcca gagagccttg ttcataggaa cgtaataatc tttataagcc tgaatatccg
      961 caactccttc ttcttttaga agttgggtca aagccacatc ctcagcagca tcaaaatcaa
     1021 gaagagtgtg gtcgaggtcg aagagtagaa atttgtagaa caatttgagg ttttcctttc
     1081 tgaaaattca ttaagaacat tatatcataa agcacctcat acaattaact aatttaatca
     1141 cttaaaaaaa ttcgaacact ttctatacaa ctgacagctc aaatctttca gaatagaaca
     1201 atactaacta tcgagcaccc cgtattcata aatatatgtg taattctaga cctagaattc
     1261 ctataaacta aatgctttca tactcttcca agtaattgat tgccttaaat tttaattttt
     1321 gaaggtttct aaagctagaa tagccccatc acaatcagtt ttgattgatt cacaatttag
     1381 aaacactata gtttcactcc tgttaaaata aaaaggaact gcataaagca atccctttct
     1441 gattttgaaa tcatttactt aacattttat agttgagata atcaatagct tacctataaa
     1501 aagagttata gtaaaattcc ttatttattg atttcaagct ccgctaactg tgtttgaata
     1561 actgacagtt ctgcaccagc ctgaagaaga gcagctgcat tataggcacc ttctacaatt
     1621 ggaaccctgt tgatgatgat acttttatca ctgaaatcag tcaccatttt taagttcatt
     1681 ttagcagaac ctaggtcaaa aaaggcaagt aaagtatctg ctgtattttc ggaaacaacc
     1741 ctatctactt aatcaaaact cgttccaatt cctccgccct cggttcctcc tacataagta
     1801 atcggaacat ctttagctac tttactaatc agttcaacaa caccttctgc aatgtgtttg
     1861 gaatgtgaaa cgataacaag accaatacca atactttcca tcaaaccacc ccagtttcta
     1921 aaatagcagt aaagagtaat cctgatgaga atgatccagg atcaatatgt ccaagaaacc
     1981 acatgctcct aagacaagag ctaacagact ggccatcaat aatagtattg ttcttttttt
     2041 catcattact ccttaactag tgtttaactg atcaattagc cagtaaatag tttatcttta
     2101 tttacactat ctgttaagat atagtaaaat gaaataagaa caggacagtc aaatcgattt
     2161 ctaacaatgt tttagaagta gaggtatact attctagttt caatctacta tattttgcac
     2221 attttcatga aaaaaatgag aactagaact cacattctgc tctcattttt cgttttcccg
     2281 ttctcctatc ctgtttttag gagttagaaa atgctgctac ctttacttac tctcctttaa
     2341 taaagccaat agtttttcag cttctgccat aatagtattg ttgtcctggg tgccaaatag
     2401 taaattattt tttaatcctg tgagagtctc tttggcattg gacttgataa ttggattctg
     2461 gatttttcca agtaaatctt cagcctctct cagtttttct ttaacctttt cagtctcgac
     2521 ctgaggttct tctgattcct ctggtgattc ttctggtgat tcttcttctg gttcctctgt
     2581 tggttttgga gactctggtt tctcgctttg cggtttctct tctcgagggg tttcttcctc
     2641 aggtttttct gtctgaggtt tctcctcgtt tggtttttcc gtttgattgg tatcagcttg
     2701 accatttttg tttctttgaa c
//
LOCUS       DAVKWZ010000113         2718 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000113 DAVKWZ010000000
VERSION     DAVKWZ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.113"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(<1..217)
                     /locus_tag="VW854_002074"
     CDS             complement(<1..217)
                     /locus_tag="VW854_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family sugar transporter"
                     /protein_id="HEV0263243.1"
                     /translation="MIGSIAAILTTFAFLPQVFRVVKTKDTGSIALGMYVMQVIGIAL
                     WLAHGIRIGDLPLILANSVSFLLSGIIL"
     gene            complement(269..1705)
                     /locus_tag="VW854_002075"
     CDS             complement(269..1705)
                     /locus_tag="VW854_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEV0263244.1"
                     /translation="MTTFKDRFLWGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVA
                     REITSGVLEGKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSIAWTRIFPKGDELEP
                     NEEGLQFYDNLFDECLKNGIEPVITLSHFEMPYHLVTEYGGWKNRKLIDFFARFAEVV
                     FKRYKDKVKYWMTFNEINNQANYQEDFAPFTNSGIVYEEGDNREAIMYQAAHYELVAS
                     ARAVKIGHEINPDFQIGCMIAMCPIYPATCNPKDILMAMKAMQKRYYFADVHVLGKYP
                     EHIFKYWERKGISVDFTDQDKEDLLGGTVDYIGFSYYMSFAIDSHRENNPYFDYLETE
                     DLVKNNYVKASEWEWQIDPEGLRYALNWFTDHYHLPLFIVENGFGAIDQVAADGMVHD
                     DYRIEYLGAHIREMKKAVVEDGVDLMGYTPWGCIDLVSAGTGEMRKRYSFIYVDKDDN
                     GKGSYNRSPKKSFGWYKEVISSNGESVE"
     gene            complement(1895..2233)
                     /locus_tag="VW854_002076"
     CDS             complement(1895..2233)
                     /locus_tag="VW854_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEV0263245.1"
                     /translation="MTLTIHVYYKGDGDSAIKFAREMLDSGLVEEIRNQKGNLKYEYF
                     LPIEKEGTILLIDQWINQETLDKHYQSKTMQKILDLRKKYHLQMQVERYIEDDSGMPE
                     SDKSFIDTGN"
     gene            complement(2389..2597)
                     /locus_tag="VW854_002077"
                     /pseudo
     CDS             complement(2389..2597)
                     /locus_tag="VW854_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          910 a          559 c          344 g          905 t
ORIGIN      
        1 ataaaattat tcctgagagt agaaatgaaa cactatttgc taaaatcaaa ggcaagtctc
       61 ctatacgaat accatgagca agccacaatg caataccaat aacctgcata acatacatac
      121 ctagagcaat agatcctgtg tcctttgtct taactacacg aaaaacttgt ggtaaaaatg
      181 caaatgttgt taaaattgct gcaatacttc caatcatatg tcacctcaat atgctaaaca
      241 aactgagaat aatctcagtt tgtttatact attctactga ttcaccgtta gatgaaataa
      301 cttccttata ccagccaaaa gattttttcg gggaacgatt ataacttccc ttcccattgt
      361 catctttatc tacataaata aagctataac gtttccgcat ttcaccggta ccagctgaaa
      421 ccaaatcaat acatccccat ggagtataac ccattaaatc aacaccatct tcaactacag
      481 cctttttcat ttcacgaata tgggcaccta gatattcaat tctataatca tcatgtacca
      541 taccatctgc tgcaacttga tctatagctc caaaaccatt ttcaacaata aagagtggta
      601 agtgatagtg gtctgtaaac caatttaacg cataacgtaa accttctgga tcaatttgcc
      661 actcccattc agaagcctta acataattat ttttcactaa atcttctgtt tcaagataat
      721 caaaataagg attattttca cgatgagagt ctatagcaaa ggacatatag taactgaaac
      781 caatgtaatc tacagtccca ccaagtaaat cttctttatc ctggtcagta aaatcaactg
      841 aaataccttt tcgttcccaa tacttgaaaa tatgctcagg atatttacct aaaacatgca
      901 catcagcaaa ataataacgc ttctgcatag ctttcattgc cattaagata tccttaggat
      961 tgcaagtagc tggataaatt ggacacatcg caatcataca acctatttga aaatctggat
     1021 taatctcatg accaattttt acagctcgtg cagaagcaac tagttcgtaa tgtgctgctt
     1081 gatacataat tgcttctcta ttatcacctt cctcatatac aatacctgag ttagtaaatg
     1141 gtgcaaaatc ttcctgataa ttcgcttgat tattgatttc attgaaagtc atccaatatt
     1201 taaccttatc tttgtaacgt ttaaatacga cttctgcaaa acgagcaaag aaatcaatca
     1261 atttcctatt tttccaacca ccatattcgg tcactaagtg ataaggcatt tcaaaatgag
     1321 atagagtgat gacaggttca ataccattct ttaagcattc atcaaaaaga ttatcataaa
     1381 actgtaatcc ttcttcattc ggctctaact catcaccttt tggaaagata cgtgtccatg
     1441 caatagaggt acggaagcac ttgaatccca tttcagcaaa aagtgctatg tcttctttat
     1501 aacgatgata aaaatctatc gcctcatgat ttggataata tttaccctct aaaactcccg
     1561 aagtaatttc acgagctact ccatgacgac cagcagtcat aacatcagca acactaattc
     1621 ctttgccacc ttcttgccat ccgccttcaa gttgatgagc agcaacagca ccaccccata
     1681 aaaatctatc tttaaaagta gtcatctttt ttcctcctga ctttgatact cttattataa
     1741 acctcaaacc aaaaaatgaa aacgtattct ttttccttat tgttaaggaa agaagtaatt
     1801 tttaatggaa atagaacaat atcttcttgt attctcgtaa tgatatcttt acgattttca
     1861 atactttcaa actacaaaaa ctctcacaat aattctaatt ccctgtgtct ataaacgact
     1921 tatcgctttc tggcatccca gaatcatctt ctatataacg ttcaacttgc atctgcaagt
     1981 gatatttttt tcttaaatct aagattttct gcattgtctt tgattgataa tgtttatcta
     2041 aagtttcttg atttatccac tgatcaataa ggagaatagt tccctctttt tcaattggta
     2101 aaaaatattc gtatttcaag ttaccttttt gatttctaat ttcttcaaca aggccactat
     2161 caagcatttc tcttgcaaac tttattgcac tatctccatc acctttataa tatacatgaa
     2221 tagtcaatgt catcttatat cctccaaaat catccttcaa ttttaaaaaa acaagtttag
     2281 atgaggatct aaacttgttt tttatgaact aattatctaa cgttttgcca ttactttcaa
     2341 tcacttcttt ataccaataa aatgattttt tcttatagcg atttatagtc aattgaaaca
     2401 agaacaagac aaaagagcct cataaaaggt attgcaactt gtaatacctt tttgaggtgc
     2461 tttttgatat gagcccatgt tttctcaata ggattgtact caggtgagta gggaggaaga
     2521 ggttaaagtt tatacccaaa ctcttcacac aagagttcta acttacccat tctatggaat
     2581 cttgcattat ccataatata gtagattgaa actagaatag tacacctcta cttctaaaac
     2641 attgttagaa ttcgatttga ttatcctcgg tttgtcctat tcttatttca cattactata
     2701 aaaataaggt aaactttt
//
LOCUS       DAVKWZ010000114         2004 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000114 DAVKWZ010000000
VERSION     DAVKWZ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.114"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            <1..225
                     /locus_tag="VW854_002078"
     CDS             <1..225
                     /locus_tag="VW854_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEV0263246.1"
                     /translation="VTTQISIQLSQELNSLLNAIVEENNTTKTDFIRQAVIEKIEDMY
                     DIKVADEAYKKWGECGKKAISHEEMMRRYG"
     gene            218..949
                     /locus_tag="VW854_002079"
     CDS             218..949
                     /locus_tag="VW854_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV0263247.1"
                     /translation="MAKLYAISDIHGYLDEFRDALNKVNLNDKDNRLFLLGDYLDNGL
                     QSFQVISKIIELEEVYPNQIITLLGNHEEWFYDWLILDKPTASAFPETIKSFFSPEEL
                     NYIFKSNANNFETGVRNEIKNNIKFNPFINWFKKRYRDKRYYETDTQIFVHAGIDEEA
                     GMLWKELTSSKIFTNKFPITTGRFHKAIISGHIASWEVAKDRRYLGKIYYDSKSHYLI
                     DGDVTNSKTIPVLCYDTITQKYHFL"
     gene            complement(1060..1143)
                     /locus_tag="VW854_002080"
     tRNA            complement(1060..1143)
                     /locus_tag="VW854_002080"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1107..1109),aa:Leu,seq:caa)
     gene            complement(1153..1224)
                     /locus_tag="VW854_002081"
     tRNA            complement(1153..1224)
                     /locus_tag="VW854_002081"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1190..1192),aa:Gln,seq:ttg)
     gene            complement(1235..1307)
                     /locus_tag="VW854_002082"
     tRNA            complement(1235..1307)
                     /locus_tag="VW854_002082"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1272..1274),aa:His,seq:gtg)
     gene            complement(1322..1392)
                     /locus_tag="VW854_002083"
     tRNA            complement(1322..1392)
                     /locus_tag="VW854_002083"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1358..1360),aa:Trp,seq:cca)
     gene            complement(1397..1477)
                     /locus_tag="VW854_002084"
     tRNA            complement(1397..1477)
                     /locus_tag="VW854_002084"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1441..1443),aa:Tyr,seq:gta)
     gene            complement(1490..1562)
                     /locus_tag="VW854_002085"
     tRNA            complement(1490..1562)
                     /locus_tag="VW854_002085"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1527..1529),aa:Phe,seq:gaa)
     gene            complement(1566..1639)
                     /locus_tag="VW854_002086"
     tRNA            complement(1566..1639)
                     /locus_tag="VW854_002086"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1603..1605),aa:Met,seq:cat)
     gene            complement(1650..1739)
                     /locus_tag="VW854_002087"
     tRNA            complement(1650..1739)
                     /locus_tag="VW854_002087"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1701..1703),aa:Ser,seq:tga)
     gene            complement(1822..1895)
                     /locus_tag="VW854_002088"
     tRNA            complement(1822..1895)
                     /locus_tag="VW854_002088"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1859..1861),aa:Ile,seq:gat)
     gene            complement(1930..2000)
                     /locus_tag="VW854_002089"
     tRNA            complement(1930..2000)
                     /locus_tag="VW854_002089"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1966..1968),aa:Gly,seq:tcc)
BASE COUNT          662 a          414 c          390 g          538 t
ORIGIN      
        1 gttactacac aaatttctat tcaacttagt caagagttaa attctttatt aaatgctatt
       61 gttgaagaaa ataatacaac aaagactgac tttattagac aagctgttat agagaaaatt
      121 gaagatatgt atgatattaa ggtcgctgat gaagcttata aaaaatgggg tgaatgtggc
      181 aaaaaagcta tctcacatga agaaatgatg agacgctatg gctaaattat acgcaatatc
      241 tgatatacat ggatacttgg atgaattcag agacgctctc aataaagtaa atttaaacga
      301 taaagataat cgcctatttt tactaggaga ttatcttgat aatggacttc aatcttttca
      361 agtcatttcc aaaataatcg aacttgagga ggtttatcct aatcaaatta ttactcttct
      421 agggaatcat gaagaatggt tttatgattg gttgatttta gataaaccta ctgcttcagc
      481 atttccagaa acaataaaaa gcttcttctc tccagaggaa ttaaactata tttttaaatc
      541 gaatgctaac aactttgaaa caggtgttag aaatgaaatt aaaaataata tcaaattcaa
      601 cccatttata aactggttta aaaagcgata tagggataaa cgctattatg aaactgatac
      661 ccaaattttt gttcatgcag gcattgatga agaagcagga atgctttgga aagaactaac
      721 gagttcaaag atatttacaa acaaatttcc aattaccact ggtagatttc ataaagctat
      781 tatttcgggc catattgctt cttgggaagt agctaaagat agaagatacc taggtaagat
      841 ttactatgat agtaaaagtc attacttaat tgatggagat gtaacgaata gcaaaacaat
      901 acctgttctt tgctatgata caattaccca aaaatatcac tttctataac ttttagtcat
      961 gtgcttttat atgaaattag taattattag taacaattcc aagaaatttc ttatgacata
     1021 acaaaaagac aaggcttata aaccttgtct tctaaactat accggcggcc ggggtcgaac
     1081 cggcacgtcc ttgcggacac tggattttga gtccagcgcg tctgccaatt ccgccacgcc
     1141 ggcaaatagt aactggggta gctggattcg aaccaacgca tgagggagtc aaagtccctt
     1201 gccttaccgc ttggctatac cccaatataa aataggcgag tgatggggat cgaacccacg
     1261 catgccagag ccacaatctg gtgtgttaac cacttcacca cacccgccat aattctatta
     1321 acacgggcag taggaattga acccacactg aaggttttgg agaccttagt tctaccttta
     1381 aactatgccc gtaaaatgga aggggaggga ttcgaacccc cgaacccgaa ggagcggatt
     1441 tacagtccgc cgcgtttagc ctcttcgcta cccttccaaa atatataaat ggcgcgagac
     1501 ggaatcgaac cgccgacaca tggagcttca atccattgct ctaccaactg agctaccgag
     1561 ccttattgcg ggagcaggat ttgaacctac gaccttcggg ttatgagccc gacgagctac
     1621 cgagctgctc catcccgcgt taatataaaa ggaggatgtg ggattcgaac ccacgcacgc
     1681 ttttacacgc ctgacggttt tcaagaccgt tcccttcagc cggacttggg taatcctcca
     1741 aaatagttta tacgggaaca agcccgtgtc ctcttagtga acagataata ttccaatccc
     1801 ctagtaatgg actaaacact atgggcacga gtggactcga accaccgacc tcacgcttat
     1861 caggcgtgcg ctctaaccac ctgagctacg cgcccaagtc aaaaacttgg tacaaagaac
     1921 aaagttcaaa gcgggtgacg agaatcgaac tcgcgacaac agcttggaag gctgtagttt
     1981 taccactaaa ctacacccgc taaa
//
LOCUS       DAVKWZ010000115         1961 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000115 DAVKWZ010000000
VERSION     DAVKWZ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.115"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(77..196)
                     /locus_tag="VW854_002090"
     CDS             complement(77..196)
                     /locus_tag="VW854_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEV0263248.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            complement(300..449)
                     /gene="blpO"
                     /locus_tag="VW854_002091"
     CDS             complement(300..449)
                     /gene="blpO"
                     /locus_tag="VW854_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HEV0263249.1"
                     /translation="MDTKMMSQFAVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            complement(693..896)
                     /gene="blpN"
                     /locus_tag="VW854_002092"
     CDS             complement(693..896)
                     /gene="blpN"
                     /locus_tag="VW854_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="HEV0263250.1"
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGRLLGP
                     WGAAIGGIGGAVVCGYLAYTATS"
     gene            complement(912..1166)
                     /gene="blpM"
                     /locus_tag="VW854_002093"
     CDS             complement(912..1166)
                     /gene="blpM"
                     /locus_tag="VW854_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="HEV0263251.1"
                     /translation="MDTKIMEQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGL
                     GTAICAASGVGAPFMGACGYIGAKFGVDLWAGVTGATGGF"
     gene            complement(1316..1961)
                     /locus_tag="VW854_002094"
                     /pseudo
     CDS             complement(1316..1961)
                     /locus_tag="VW854_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          620 a          437 c          272 g          632 t
ORIGIN      
        1 attttgacag gcgaaacaat attaaagaaa ctcccctgta aattaagcta gcaaatacag
       61 gggagaaatt tattttttag agagtactat ccgtatcctt tttggaagat tttgaaaata
      121 tttttctaat taagtcatcc atataaggac caaatatacc aactactaaa ccaataataa
      181 aacttttaaa atccataatt accaccaaca tattgctgca taggctacac ctccaagtat
      241 agctccacct gcagcaccag ttacacctat tcctatagca aatggtccca atagaaatgt
      301 caaaccgttg ttgcacaccc atcaattgcg ccatatgcaa cccctgctgc acaactaatt
      361 tttcttcccc aatcaatatc tccaccttca acgcaagcaa gcatttcatt atccataact
      421 gcaaattgtg acatcatttt tgtatccata tagtatatca cttttcagtt acggaacaag
      481 tttaatataa aaattatcaa aaaaacatag gcaataaaga gaaaaattaa tttatcatag
      541 attagaaata atatgacaaa acaattcaat gatgttaatt caatagtctt ttgtttttta
      601 tcggagatac ttatggatag ataaataaga taggtttgaa aagcgaagag aataataaag
      661 aatatagcct tcataaaatt tagctttcat ttttatgatg tagcggtata ggctaaatat
      721 ccacaaacca ctgctcctcc aattcctcct attgcagcgc cccatggtcc tagaagtctc
      781 ccatatttca ctccacccgc tgcacaacct aaagcagcaa ctacagctgc tcctccggaa
      841 ttacctccat aaacctcact cagcattgtt tcatttatat tacaataagt attcatacaa
      901 gtctcctttt attaaaatcc acccgttgcc cctgttactc ctgcccaaag atccacacca
      961 aatttagctc ctatgtatcc acatgctccc ataaatggtg ctccaacacc actcgcagca
     1021 caaatagctg tccctagccc ccagccacca aaagcagcac caccaccttc taagacatta
     1081 gtttgccaat tattcttgcc tccttcaata ctagataaca tagttatatc catttcatga
     1141 aattgttcca taatttttgt atccatgaca aatactcttt tttattttta atttttgtct
     1201 tgttgtaact ttgacaagtt tagtatatca tcgtttttta aaatttttca tccagatctt
     1261 gaatagtcat cgaaacgtct tgaattgcaa aaattacatt agacttcctg caaaactaga
     1321 atcctagttc atgattgata ataccaacac tcaaattcat tcgtaatccg aagcgtttac
     1381 gatgatttcg ataggttgtt gcaaacattt taaacgtttt tactttggca aagatgttct
     1441 caaccttgct tctctcctta gatagcgcat ggttacaggc tttatcttca actgttagcg
     1501 gtttgagttt gctggattta cgtgaagttt gtgcttgagg atatatcttc atgagccctt
     1561 gataaccact gtcagccaag atttttccag cttgtccgat atttctgcga ctcattttga
     1621 acaacttcat atcatgacaa tagttcacag tgatatccaa agaaacaatt ctcccttgac
     1681 ttgtgacaat cgcttgagtc ttcatagcgt gaaatttctt tttaccagaa taattcgcta
     1741 attctttttt tagggcgatt gatttttacc tctgttgcat ccacaatcac cgtatcctca
     1801 gcactaagat gagtttttga aatcgtaaca ccactttgaa caagagttac ttcaacccat
     1861 tggctccgac ggattaagtt gctttcgtga ataccaagat cagccgcaat ttcttcataa
     1921 gtgcggttct cacacatatt gaagagtggc cataagaagg t
//
LOCUS       DAVKWZ010000116         1957 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000116 DAVKWZ010000000
VERSION     DAVKWZ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.116"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            <1..407
                     /locus_tag="VW854_002095"
     CDS             <1..407
                     /locus_tag="VW854_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263252.1"
                     /translation="RVLGIVNFFKYYESSKFHLFFGDAELAFGDMTKGYTHNIRSVLG
                     WDGTVEMPLLSVMIKNGYVGLIGYGVVLFKFISSVLSMEDRRVKNIGLSILIPLLLSA
                     MVENYIVNISFVFMPVCFCILCSIKNIEFKNN"
     gene            417..1385
                     /locus_tag="VW854_002096"
     CDS             417..1385
                     /locus_tag="VW854_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV0263253.1"
                     /translation="MKKVSIILPVYNVEQYIKKCLESIQQQTYPNLEVIIVNDGATDK
                     SVEYCEQICKIDSRFSVTHKENGGLSDARNVGIDKAKGDYLIFVDSDDFVSQDMVSYL
                     VSSMENNEADIAICDPAHYYSDRQNNDLNIFYPASSVKVYEKTEALCEMFYQKSFLVS
                     AWAKIYKKELFDDIRFPVGKLFEDSAVMYLLFEKCEKIVYSNAKLYAYVHRDNSITTK
                     KFSDKDLDILDISNTILDHYSGNFRVYKAAVSYKVSACFRILLNSSSEKKYNQIQKDC
                     MTYILRNWRNVLFDKNVRLKNKLALISITLFNPFVKFIYSKVNRWE"
     gene            1392..>1957
                     /locus_tag="VW854_002097"
     CDS             1392..>1957
                     /locus_tag="VW854_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 61 protein"
                     /protein_id="HEV0263254.1"
                     /translation="MNKYEERYQEDLSKNDFEKLINRRYLSDKELQVEYVKKGTVLPP
                     KVFEMKLSNKLGLQKALHGKGGVVDSKGNYVELSEQKAVGMRNRVYGSYKFNHKNLAI
                     RNEKVIYLNYFINQWGHFLLDVVGRLWYPLLKDTDTKLDYTCYAGTETKLEGNYLEFL
                     ELLGIDKSRLILINRPTQFSEIIIPESS"
BASE COUNT          697 a          244 c          357 g          659 t
ORIGIN      
        1 atcgtgtttt gggtattgtt aattttttta aatactacga atctagcaaa tttcatttat
       61 tttttggtga tgctgagtta gcctttggtg atatgacgaa aggttatact cacaatataa
      121 gaagtgtttt aggttgggat ggaactgtgg aaatgccgtt gctaagtgtt atgataaaaa
      181 atggttatgt cggcttaata gggtatggag tagtcttatt taaatttata tcctctgtat
      241 tatctatgga agatagaaga gtaaaaaata ttggcttatc cattctaata cctctacttc
      301 tatcagcaat ggttgaaaat tatatcgtta atattagctt tgtattcatg ccggtctgtt
      361 tttgtatttt atgctcaata aaaaatatag aatttaaaaa taattgaaag tgatttatga
      421 aaaaagtaag tattattcta cctgtctaca atgttgaaca gtatataaaa aaatgcttag
      481 aatcaattca acaacaaact tatccaaatt tggaagtaat cattgttaat gacggagcaa
      541 ccgataaaag cgttgaatac tgtgagcaaa tttgtaagat agattctagg ttttctgtca
      601 ctcacaaaga aaatggtgga ctttccgatg caagaaatgt cggtatagat aaagccaagg
      661 gagattacct catttttgtt gacagtgatg attttgtctc tcaagatatg gtatcttatt
      721 tagtatctag tatggagaac aatgaggcag acattgctat ttgcgatcct gctcattatt
      781 atagtgaccg tcaaaacaat gatcttaata ttttttatcc ggcatcaagc gttaaggttt
      841 acgaaaaaac ggaagctcta tgtgagatgt tttatcaaaa atcttttttg gtatctgctt
      901 gggctaagat atataaaaag gaactatttg atgatatcag attccctgtt gggaagttat
      961 ttgaagatag tgctgttatg tatctgcttt ttgaaaaatg tgaaaagata gtatattcta
     1021 atgcgaaatt atatgcctat gtgcatagag acaatagtat tacaacaaag aagttttcag
     1081 ataaagattt agatatttta gacatttcaa atactattct tgatcactat agtgggaact
     1141 ttagagttta taaagcggca gtttcctata aagtttcggc atgttttaga attttgttga
     1201 actcatcttc agaaaaaaaa tataatcaaa tacaaaaaga ttgtatgaca tatattttac
     1261 gaaattggag aaatgtgtta tttgataaaa atgtccgatt aaagaacaag cttgccttga
     1321 tttctataac tcttttcaat ccttttgtta aatttatcta tagcaaagta aacagatggg
     1381 aataatagca aatgaacaaa tatgaagaaa gatatcagga agatttatct aagaatgatt
     1441 ttgagaaact aatcaataga agatacctat cggataaaga actacaagtt gaatatgtaa
     1501 aaaaaggaac agtgttacct cccaaggtct ttgaaatgaa actatcaaat aaacttggtc
     1561 tacaaaaagc tttgcatggg aaaggtggtg ttgtagattc aaaggggaat tatgttgaac
     1621 tttctgaaca aaaagcagtt gggatgagaa atcgagtcta tggatcttac aaatttaacc
     1681 ataaaaattt ggctatcaga aatgaaaaag ttatttacct aaactatttc ataaatcagt
     1741 ggggacattt tttactagat gttgtgggga gattatggta tccattacta aaagatactg
     1801 atactaaact ggattacaca tgttatgctg gtacagaaac gaaacttgaa ggaaactatt
     1861 tagagttttt agaactcctc ggtatagata aatcacgctt aattttgata aatcgcccaa
     1921 ctcaattctc agagataatt atacctgaaa gttcaat
//
LOCUS       DAVKWZ010000117         1935 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000117 DAVKWZ010000000
VERSION     DAVKWZ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.117"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            40..1584
                     /locus_tag="VW854_002098"
     CDS             40..1584
                     /locus_tag="VW854_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0263255.1"
                     /translation="MLIYAATINALVLNELIAMNLERFLKLSIYQMIVWCGIIFLDWV
                     VKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHSKSSGTYLSWLNNDVQTLNDQAFKQ
                     LFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLMIMLLVPKIFASKMREVSLNLTSQN
                     EAFLKSSETILNGFDVLASLNLLYVLPKKIKEAGILLKMVIQRKTTVETLAGAISFFL
                     NIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTGVIFTALGELGGQLSSIIGTKPIFL
                     KLYSINPIESNKMNDIEPNEVNRDFPLYEAKNICYKYGDKEILKNLNFCFQRNEKYLI
                     LGESGSGKSTLLKLLNGFLRDYSGELRFCGDDIKKTSYLNMVSNVLYVDQKAYLFEGT
                     IRDNILLEENYTDEEILQSLEQVGLSVKDFPNNILDYYVGDDGRLLSGGQKQKITLAR
                     GLIRNKKIVLIDEGTSAIDRRTSLAIERKILDREDLTVIIVTHAPHPELKQYFTKIYQ
                     FPKDFI"
BASE COUNT          675 a          236 c          356 g          668 t
ORIGIN      
        1 tttagtttat taacgatatt tattgttgct ggtcaattat tgctaattta tgcagcaact
       61 attaatgctc tggtgttgaa tgaattaatt gcgatgaatt tagagcggtt tttgaaattg
      121 tcaatctacc aaatgattgt ctggtgtggg ataatattcc ttgactgggt agtgaaaaat
      181 tatcaggttg aagtgatcca agagtttaat ctagagattc gaaatagagt tgccacagac
      241 atctctaact ctacctatca agaatttcat agtaaatcat caggaacata tctttcgtgg
      301 ctaaataatg atgttcagac tttaaatgat caggctttta aacaactttt tttagtaata
      361 aaaggaattt ctggtactat atttgcagtt gtgactctta atcactatca ttggtcattg
      421 actgtagcca ccttgttttc attaatgatt atgctacttg taccaaaaat ctttgcatcg
      481 aaaatgcgag aagttagtct aaatttaact agccaaaatg aagctttttt aaaatctagt
      541 gagactatat tgaatggatt tgatgtgtta gcgtccttga atcttttata tgtattgcct
      601 aagaaaatta aagaagcagg aattttatta aagatggtta tacaaagaaa gacaactgta
      661 gaaacgttag caggcgctat tagcttcttt ctcaatattt tttttcagat atctctcgtt
      721 tttttaacag gctatcttgc aataaaagga atagtgaaaa ttggtactat tgaagcaata
      781 ggagcactaa caggtgttat ttttacagcg ctaggtgaat taggaggtca attatcctct
      841 attattggta cgaagcctat ttttttaaaa ttgtattcaa ttaatccaat tgagtcaaat
      901 aaaatgaatg atatcgaacc aaatgaggtg aatagagatt ttccgttata tgaagcaaaa
      961 aatatttgct ataagtatgg agataaagaa atattaaaaa acttaaattt ttgttttcaa
     1021 cgtaatgaaa agtatttaat tttaggtgaa agtggaagcg ggaaatctac attattaaaa
     1081 ttattgaatg gctttttgag agattatagt ggagaattgc gattctgcgg ggatgatata
     1141 aaaaaaacct cctatttaaa tatggtttcg aatgttctat atgtagatca aaaagcttat
     1201 ttgtttgaag gtacgattag agataatatt ttattggaag aaaattatac tgatgaagaa
     1261 atactacagt ctttagaaca agttggtttg agtgtaaaag attttcctaa taacatttta
     1321 gattattatg ttggtgatga tgggagatta ctgtcaggag ggcagaaaca aaaaattact
     1381 ttagctagag gcctaattag aaataagaaa atagtattaa ttgacgaggg aacttctgct
     1441 atcgatagga gaacttcgtt agcgattgaa cgtaagatat tagatagaga ggatttgact
     1501 gtcattattg ttacccatgc tccgcatccg gaacttaaac aatattttac taagatatat
     1561 caatttccaa aggattttat ttaagaatat aggggaggga aacatgagat tctattgtga
     1621 agcttatgag gtaattactg aagagaaaaa agtatcattt ttttatgtag atgagaaacc
     1681 cttaaggtat ctgttatttg actatatatt tctactagtt ctagctaaaa aaaaatatcc
     1741 caatcttatg aactgcaccc caaaagttag atttattctg tctaactttt ggggtgcagt
     1801 tcattttgca tagttttggc attcatccta gatatccata taaaatgtct gagattgttg
     1861 aaatgtctaa aaaaatatca aatctaaacg ataaaaaaga atttcacaaa aagaactgtg
     1921 agttgaggtt tcaag
//
LOCUS       DAVKWZ010000118         1927 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000118 DAVKWZ010000000
VERSION     DAVKWZ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.118"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            200..469
                     /gene="blpJ"
                     /locus_tag="VW854_002099"
     CDS             200..469
                     /gene="blpJ"
                     /locus_tag="VW854_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="HEV0263256.1"
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITG
                     GTGAGLATLGVAGLAGAFVGAHIGAIGGGLTCLGGMVGDKLGLSW"
     gene            538..768
                     /gene="blpU"
                     /locus_tag="VW854_002100"
     CDS             538..768
                     /gene="blpU"
                     /locus_tag="VW854_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HEV0263257.1"
                     /translation="MDTKMMSQFSVMDTEMLACVEGGGCNWGDFAKAGVGGAAARGLQ
                     LGIKTGTWQGAATGAAGGAILGGVAYAATCWW"
     gene            771..890
                     /locus_tag="VW854_002101"
     CDS             771..890
                     /locus_tag="VW854_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEV0263258.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDNLIRKIFSKFPKKNTDSTP"
     gene            1358..1729
                     /locus_tag="VW854_002102"
     CDS             1358..1729
                     /locus_tag="VW854_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263259.1"
                     /translation="MIKKNHILCEIVPGSVAFACFYLSISISSVFQFLLVVINLASIL
                     VTVYLTREMRIKEFEDDDLVSPRTNQIMFIGLTGFMSIICLYRGITAGESYQQIIAYI
                     GAILCLIIILFLIWGLKYYKK"
BASE COUNT          610 a          250 c          391 g          676 t
ORIGIN      
        1 ggagaataac gaaattaaga tattttagat actcaagatt attgaaatct tagactaaaa
       61 aataggtact gctaccttta agaagatagt agacgtatat acttttttaa gaaaatcaaa
      121 aagatactat aaaaaatcta tttgtttatt gaatttaaga ctttggtaac aaattgaaaa
      181 taaaaaggag gtattcatca tgaatacaaa aatgttgtca cagttagagg ttatggatac
      241 tgaaatgctt gcgaaagttg aaggggggta tagctccact gattgtcaaa atgcactgat
      301 tacaggagtc actacgggaa ttataactgg tggaactgga gcaggtttgg ccactttagg
      361 agtagctgga cttgctggtg catttgttgg agcacacatt ggtgcaattg ggggtggatt
      421 aacatgctta ggaggtatgg ttggtgataa attgggacta agttggtaag acttatagta
      481 aaattatgag gattctattc tgatttattt tagtttttta aaaaaggagt atttgtcatg
      541 gatacaaaaa tgatgtcaca attttctgtt atggatactg aaatgcttgc ttgcgttgaa
      601 ggtggcggat gcaattgggg agattttgcc aaagcaggtg ttggaggagc agcagcaaga
      661 ggtcttcaac taggcattaa aacaggaaca tggcaaggtg cagcaactgg tgctgcgggt
      721 ggagctatac ttggaggtgt agcctatgca gcaacatgtt ggtggtaatt atggatttta
      781 aaagttttat tattggttta gtagttggta tatttggtcc ttatatggat aatttaatta
      841 gaaaaatatt ttcaaaattt cccaaaaaga atacagatag taccccctaa aaaataaatt
      901 tctcccctgt atttgctagc ttaatttaca ggggagtttc tttaatattg tttcgcctgt
      961 caaaatgata tgtgatagca ggatgaaaag tctacaaaca agttggtatc aatacaatat
     1021 aaagaggaaa gtatgttttt catgcttgct tttttaattt ttacaattca agaggttttg
     1081 atgaccattt atgatttgag tgatccaagg tcaaaataag tgctatacta gcagtgtaaa
     1141 tgttcttgct caactaagat aaatcgcaca tttaggagga aatcttatga agaaaaaaat
     1201 actgattatt ttcgttctgt atctgatcat gtccatcttt ctttatccgc ttagggagag
     1261 tatttggtat aatctatttt ataccatagc ctatatgatt gcggttatga tctatttttc
     1321 tttaattaga aagaaagaaa agaaatgaaa gattttcttg attaaaaaga atcatattct
     1381 ttgtgagatt gttcctgggt cagttgcctt tgcttgtttc tacttatcta tttctatttc
     1441 gtcagttttc caatttcttt tagtggttat taacttagct tctattttgg tcactgttta
     1501 cctcactagg gaaatgcgga taaaagagtt tgaagacgat gatttggtta gtcctagaac
     1561 taatcaaatc atgttcatcg gcttgacagg ttttatgtct attatttgtt tgtatagagg
     1621 gatcacagca ggagaatcct accaacaaat aattgcctac attggtgcta ttctctgctt
     1681 gattattata ctttttctca tttggggctt gaagtattat aaaaagtaga gcttaataaa
     1741 gtataaaggg gtaagtgttt ttgaaatact taccctttta taatagattc cctgcgaaac
     1801 aaaatatggt atagtatatt gaaactagaa tagtacgcct ctacttctaa aacattgtta
     1861 gaaatcgatt tgactgtcct gatcgattta tcctgttctt atttcatttt actatagttc
     1921 tatgaat
//
LOCUS       DAVKWZ010000119         1873 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000119 DAVKWZ010000000
VERSION     DAVKWZ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.119"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            <1..310
                     /locus_tag="VW854_002103"
     CDS             <1..310
                     /locus_tag="VW854_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0263260.1"
                     /translation="PDCGSLMKKKEPEKFFGLIEDNLKQVHPIFQTVFKTFLKDKEKI
                     VNALQLHYSNAKLEATNNLIKLIKCNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA
                     "
     gene            complement(444..531)
                     /locus_tag="VW854_002104"
     tRNA            complement(444..531)
                     /locus_tag="VW854_002104"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(495..497),aa:Ser,seq:gct)
     gene            complement(538..611)
                     /locus_tag="VW854_002105"
     tRNA            complement(538..611)
                     /locus_tag="VW854_002105"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(575..577),aa:Ile,seq:gat)
     gene            complement(647..717)
                     /locus_tag="VW854_002106"
     tRNA            complement(647..717)
                     /locus_tag="VW854_002106"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(683..685),aa:Gly,seq:tcc)
     gene            complement(737..809)
                     /locus_tag="VW854_002107"
     tRNA            complement(737..809)
                     /locus_tag="VW854_002107"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(774..776),aa:Phe,seq:gaa)
     gene            complement(813..886)
                     /locus_tag="VW854_002108"
     tRNA            complement(813..886)
                     /locus_tag="VW854_002108"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(850..852),aa:Met,seq:cat)
     gene            complement(900..989)
                     /locus_tag="VW854_002109"
     tRNA            complement(900..989)
                     /locus_tag="VW854_002109"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(951..953),aa:Ser,seq:tga)
     gene            complement(1000..1073)
                     /locus_tag="VW854_002110"
     tRNA            complement(1000..1073)
                     /locus_tag="VW854_002110"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1037..1039),aa:Ile,seq:cat)
     gene            complement(1087..1160)
                     /locus_tag="VW854_002111"
     tRNA            complement(1087..1160)
                     /locus_tag="VW854_002111"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1124..1126),aa:Met,seq:cat)
     gene            complement(1166..1239)
                     /locus_tag="VW854_002112"
     tRNA            complement(1166..1239)
                     /locus_tag="VW854_002112"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1203..1205),aa:Pro,seq:tgg)
     gene            complement(1255..1328)
                     /locus_tag="VW854_002113"
     tRNA            complement(1255..1328)
                     /locus_tag="VW854_002113"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1292..1294),aa:Arg,seq:acg)
     gene            complement(1338..1423)
                     /locus_tag="VW854_002114"
     tRNA            complement(1338..1423)
                     /locus_tag="VW854_002114"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1387..1389),aa:Leu,seq:taa)
     gene            complement(1431..1502)
                     /locus_tag="VW854_002115"
     tRNA            complement(1431..1502)
                     /locus_tag="VW854_002115"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1468..1470),aa:Gly,seq:gcc)
     gene            complement(1525..1597)
                     /locus_tag="VW854_002116"
     tRNA            complement(1525..1597)
                     /locus_tag="VW854_002116"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1562..1564),aa:Thr,seq:tgt)
     gene            complement(1616..1697)
                     /locus_tag="VW854_002117"
     tRNA            complement(1616..1697)
                     /locus_tag="VW854_002117"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1661..1663),aa:Leu,seq:tag)
     gene            complement(1703..1775)
                     /locus_tag="VW854_002118"
     tRNA            complement(1703..1775)
                     /locus_tag="VW854_002118"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1740..1742),aa:Lys,seq:ttt)
     gene            complement(1800..1872)
                     /locus_tag="VW854_002119"
     tRNA            complement(1800..1872)
                     /locus_tag="VW854_002119"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1837..1839),aa:Asp,seq:gtc)
BASE COUNT          548 a          494 c          386 g          445 t
ORIGIN      
        1 ccctgattgt ggaagtctaa tgaagaaaaa ggaaccagag aaatttttcg gacttattga
       61 ggacaatctt aagcaggttc atcctatttt tcagactgtc tttaaaacct tcctcaaaga
      121 taaagaaaag attgtcaacg cccttcaact acactattct aacgccaaac tggaagcgac
      181 caataatctc atcaaactta tcaagtgcaa tgcctttggt tttcgaaact ttgaaaactt
      241 taaaaaacgg atttttatcg ctttgaacat caaaaaagaa aggacgaaat ttgtcctttc
      301 tcgagcttag cttttcttca acccactaca gttgacaaag agccataaaa ataacttcag
      361 attagtaatt caattaagtt ttacggatct gaagctttat tgtctctaaa aataaaaaag
      421 agagttacaa actctcatta aaacggagaa taagggattc gaacccttgc gccagttacc
      481 cgacctaacg atttagcaaa ccgtcctctt cagcctcttg agtaattctc caattaatgg
      541 gcacgagtgg actcgaacca ccgacctcac gcttatcagg cgtgcgctct aaccacctga
      601 gctacgcgcc caagttaaaa aacttggtaa tttgaacaaa gttcaaagcg ggtgacgaga
      661 atcgaactcg cgacaacagc ttggaaggct gtagttttac cactaaacta cacccgcata
      721 aatactatca ataaaatggc gcgagacgga atcgaaccgc cgacacatgg agcttcaatc
      781 cattgctcta ccaactgagc taccgagcct tattgcggga gcaggatttg aacctacgac
      841 cttcgggtta tgagcccgac gagctaccga gctgctccat cccgcgttaa taatataaaa
      901 ggaggatgtg ggattcgaac ccacgcacgc ttttacacgc ctgacggttt tcaagaccgt
      961 tcccttcagc cggacttggg taatcctcca atattcaaat ggaccttgta ggacttgaac
     1021 ctacgaccac tcggttatga gccgagagct ctaaccagct gagctaaagg tccgacaaga
     1081 tcattatagc ggcgaagggg atcgaacccc cgacctcccg ggtatgaacc ggacgctcta
     1141 gccagctgag ctacaccgcc atgaatcggg aagacaggat tcgaacctgc gacaccttgg
     1201 tcccaaacca agtactctac caagctgagc tacttcccga gttaaataga aaaatgcacc
     1261 ctagaggagt cgaacctcta accgcctgat tcgtagtcag gtactctatc cagttgagct
     1321 aagggtgctc catattatgc cgaggaccgg aatcgaaccg gtacgatcgt taccaatcgc
     1381 aggattttaa gtcctgtgcg tctgccagtt ccgccacccc ggcctctcta agcgaacgac
     1441 gggattcgaa cccgcgaccc ccaccttggc aaggtggtgt tctaccactg aactacgttc
     1501 gcactgtttt cttctatcta aaaatgccgg ctacatgact tgaacacgcg accctctgat
     1561 tacaaatcag atgctctacc aactgagcta agccggctca tttgttatat cttaatgcgg
     1621 gttaagggac ttgaaccccc acgccgttaa gcgccagatc ctaaatctgg tgcgtctgcc
     1681 aattccgcca aacccgcata tatgacccgt actgggctcg aaccagtgac ccattgatta
     1741 aaagtcaatt gctctaccaa ctgagctaac gagtctaaaa taacttgcgt taccttaaac
     1801 ggtcccgacg ggaatcgaac ccgcgatctt cgccgtgaca gggcgacgtg ataaccgcta
     1861 cactacggga cct
//
LOCUS       DAVKWZ010000120         1776 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000120 DAVKWZ010000000
VERSION     DAVKWZ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.120"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(33..269)
                     /locus_tag="VW854_002120"
     CDS             complement(33..269)
                     /locus_tag="VW854_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HEV0263261.1"
                     /translation="MLQKIYEQMANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCHRDFIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            535..1290
                     /locus_tag="VW854_002121"
                     /pseudo
     CDS             535..1290
                     /locus_tag="VW854_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1325..1534)
                     /locus_tag="VW854_002122"
                     /pseudo
     CDS             complement(1325..1534)
                     /locus_tag="VW854_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1700..1773)
                     /locus_tag="VW854_002123"
     tRNA            complement(1700..1773)
                     /locus_tag="VW854_002123"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1737..1739),aa:Asn,seq:gtt)
BASE COUNT          633 a          350 c          251 g          542 t
ORIGIN      
        1 gtaaattagt tcggaaattt actaaaatta cctcaacaag aaataaaacc ccgattcatt
       61 accaattttt caagatacaa acgataagca acacgataat ggtaaacgat aaaatcccta
      121 tgacaaccaa tgccatatct cactaaataa taaattaaaa atttaaaatg aacatgttcc
      181 cagtcaaaat tatcaccaaa tgtaggacca tactcttctt caatactatc atagaaatta
      241 gccatctgct cataaatttt ttgtaacata atcaaatact ccttttcttt tttataaact
      301 tattctaaca aaaaatttta cacattcact atcaattcct gaattgttaa aataccagcc
      361 tcaacaagat aaaaatagga aaagttgaca aaatagaaac aaatttgcta cctaaatttc
      421 aaacaattaa agttctaaaa agaccgcctt aaaacttttg ggtaaatcct attagaacta
      481 taatattttt atataggtat tatggttctg attttctagt ataaaaaaat tcaaactaga
      541 attcttctta gaaaaggccg ttttaagtgc tatcattgct caaaaatgga ggttgctgag
      601 acttctatcg tcaagaagaa tcatcaaatt ccttgtatca tcaaccaaaa gattgctcag
      661 aagctaattg aaaagacttc tatgaccgac attgatcatc agttgtctat ttcaacttca
      721 actgtcattc gcaagatcaa tgattttcac tttgagcatg atttttcgcg tcttcctgag
      781 attatgtcct aggacgttga aacagtctgg ggagtgactg tttcaatcgg gagatagaga
      841 tgagctttat tgctcaggac tttgataagc tcaatatcat aactgttctt gagagcagaa
      901 cacaagccat catccgaaat cccatgaata caaggctatc aagcgatact gaaagctcat
      961 tcaacaagat agtcagaaac tgagtgataa agaaattcta gataaacttt tgagctattc
     1021 cgaagacttg aaacaccact ataatctcta tcagctcttg cttttccacc tctttttcag
     1081 actctgttta aaaccttcct caaagataaa gagaaaatcg tcaacgccct tcaattacct
     1141 tattccaacg ccaaactgga agccaccaat aatctcatca aacttatcaa gcacaatgcc
     1201 tttggtttta ggaactttga aaacttcaaa aaagaaagga cgaaatttgt cctttctaga
     1261 tcttcgcttt cttcaaccca ctacagttga caaagaaccg aaaaatgtct cgcctctaat
     1321 atagtcaatt gaaacaagaa caagacaaaa gagcctcgta aaaggtattg caacttggta
     1381 ataccttttt gaggtgcttt ttgatatgag cccatgtttt ctcaatagga ttgtactcag
     1441 gtgagtaggg aggaagatgt aaaagtttat gcccaaactc ttcgcataaa agttctagct
     1501 tccccattct gtggaatctt gcattgtcca taatatagta gattgaaact agaatagtac
     1561 acctctactt ctaaaacatt gttagaattc gattttactg tcctaatcga tttgtcctat
     1621 tcttatttca ttttactata acacgaaata gattattatt acataacaaa aaagaggtct
     1681 aaacctctta actcaattac tccgccagta ggactcgaac ctacgacatc atgattaaca
     1741 gtcatgcgct actaccaact gagctatggc ggatta
//
LOCUS       DAVKWZ010000121         1757 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000121 DAVKWZ010000000
VERSION     DAVKWZ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.121"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(1..593)
                     /locus_tag="VW854_002124"
                     /pseudo
     CDS             complement(1..593)
                     /locus_tag="VW854_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            670..1125
                     /locus_tag="VW854_002125"
     CDS             670..1125
                     /locus_tag="VW854_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV0263262.1"
                     /translation="MQWQTQLSEKKANTDYIARYGVYAVIPNPEQKQIVLVQEPNGAW
                     FLPCGEIEAGENHQEALKRELIEELGFTAEIGTYYGQADEYFYSRHRDTYYSNPAYFY
                     EATPFKEVQKPLENFNHIAWFPIDEAIKNLKRGSHKWAIESWKKQHKID"
     gene            1290..1589
                     /locus_tag="VW854_002126"
     CDS             1290..1589
                     /locus_tag="VW854_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEV0263263.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKTSVDEANIKTLYSEKLIGISIPIK"
BASE COUNT          575 a          343 c          299 g          540 t
ORIGIN      
        1 aaccaagctt caaaaaagtc gctcgtcatc gtctcttcgt aagtcattgg agcgattaat
       61 tcaccatttg ttagacctgc aaccaaagaa atcctctgat atcttcttcc agatactttg
      121 cctcttatta actgaccttt taatgagcga ccatattctc gataaaaata agtatcgaat
      181 cctgtttcgt caatctaaac aggtgctagg tgctttaaac tattaaaatt cttaagaaat
      241 aaggctactt tttctgggtc ttgttcatag taggtgtggt tcttttttcg agtgtagccc
      301 atagctttga gcgcatagtg gatggtagtt ggatgacagc caaattcaga agctatttca
      361 gtcaaataag cgtctggatt gtcagtaaga tagtttttaa gtctatctct atcaactttt
      421 cttggttttg ttccttttac ttggtggttt agctctcctg ttttctcttt tagctttaac
      481 cagccataaa tggtattacg tgagatttgg aaaacgtgtg atgcttctgt tatactacct
      541 attcgctcac aataagagag aactttttta cgaaaatcta ttgaatatgc cataagaaga
      601 ttataccaca ttgtgtacta tttttggttc atttcactat aataataacg atactagata
      661 atgaggaaaa tgcaatggca aacccaactt tcggagaaaa aagcgaatac agactatatt
      721 gctcgctatg gcgtatatgc agttatccct aatcctgaac aaaaacaaat tgttcttgtt
      781 caagaaccaa atggtgcttg gttcctacca tgtggagaaa ttgaagcagg tgaaaatcat
      841 caggaagccc taaagcgtga gttgattgaa gagcttggtt tcacagcaga aattggtacc
      901 tattacggac aagctgacga atatttctat tctcgtcatc gtgataccta ctactccaat
      961 cctgcctact tctatgaagc aactcctttc aaagaagtac aaaagccact agaaaacttt
     1021 aatcatattg cctggttccc tattgacgag gctatcaaaa accttaaacg tggtagccat
     1081 aaatgggcca ttgaatcttg gaaaaaacag cataagattg actaatactc ttcgaaaata
     1141 aaattcaaac cacatcagcg tcgccttacc atactcaagt atagcctgcg gctagcttcc
     1201 tagtttgctc ttttattttc attgagtata aatcaagcga ctttattcaa aaagtgctat
     1261 aatagaatta gaaaaccgga ggaaatatta tgaagcttat caatacgaca aactcacact
     1321 cgcaacttgt aaaaagtcag ctagaaagta ccgatgcaac ccttgttgag gtttattctg
     1381 cagggaatac agatgttatt tttacccaag ctccgcttca ctatgaaatc ctcatctcaa
     1441 acaaacaccg tgctattcgt gagcctgaaa tcgaaaccat tcaagaattt ttcttgaaac
     1501 gtaaaattga taagacctct gttgatgaag ccaatatcaa gacactctac tcagagaaat
     1561 taattggaat ttcaatccca atcaagtaag aaaattgagt aaaaactcga tttgagagaa
     1621 agtccaataa gtctttccat aacaaaacgc atagtaacaa gagtttctac acccactatt
     1681 atgcgttttt atcattttga aatctactgc aatcaatgaa gaagcaaaga tcaaactagg
     1741 cagctagtta caagtta
//
LOCUS       DAVKWZ010000122         1745 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000122 DAVKWZ010000000
VERSION     DAVKWZ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.122"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            27..1028
                     /locus_tag="VW854_002127"
     CDS             27..1028
                     /locus_tag="VW854_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV0263264.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     misc_binding    1240..1469
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT          581 a          232 c          354 g          578 t
ORIGIN      
        1 tagaatgttt gtaatagagg actgaaatga aaaatataaa tgggaaaaag gtaactattt
       61 atgatattgc acgtttatca ggtttttctc caaaaacagt ttcacgagtt attaatggtg
      121 gagtaaatgt taaagaagaa acttatcaag cgattcaaaa agttatagag gaattgtcgt
      181 atattccaaa tgcgtatgca aaaaatttaa ctaaaaaaga agctataaat atattgattt
      241 ctgtaaaaaa aatcgactct tttcctttaa tttggtttca tacgctgtta gataaagtgt
      301 tacggacgtg taaagaattt ggtgttaatg ctatagttga gtactttgga gaggaggata
      361 caattagtaa ttcaattata tcaagcacag gtagcctagt agatggtgtt attgtttttt
      421 atgaaagtgt agatgatatc agaattcagt atttaaagaa gaaccatatg ccttttcttg
      481 ttttcggtga atctcaaacg tctggagtag tctatgtatc taataataat tttcaagcta
      541 cttatgatat gatgaaagct gtaactgaag aaaaatttaa aaatatgtgg ttgcttatgg
      601 gaggagaatc tcatgttaat aaggatcgtg aaagaggtgt gcgttctttt ctgaatgata
      661 aaaattactt tatggattta aaagtcattt atggcttatc tacaattgat tcagtttatt
      721 cttatgcaat gcagcacttg actacccaaa attatccgga cattattttt gtttcaggag
      781 atgaaaaggt tcagggcttg attcgtgcgt gctatgagaa aggaattctt attccagatg
      841 atatttctat tattggattt gataatattc ctatttctca gtattatact ccagctttgt
      901 ctacaattgc tcccaattat gttaaattgg ctaaagaaat gatagaaggt gttttagcaa
      961 ttattaaagg ggaaagtgtc acatctgttg aagtttctcc taaatttgtt aggagacaga
     1021 gtttctagta ttgtttaatt cttgaaaatc ctagtggaaa agatggagtg ttctaaaata
     1081 agttctgttt agcctccatt cagtttctat tgagttggat ccaatttcta acaagtggga
     1141 catgttcatg ttcgatctaa ggaagacatg gtcacattcg aatgatattt tcaaaaaaat
     1201 cgtaaaaaag tcttgacaaa gaaaaaacaa agtattaaaa taagttcaac aaatcagaaa
     1261 agtaactatt tttgatcttc agggagcctg tggtggttgt gaataggtgg ttggaagtag
     1321 tgaaagtggg ctgattttga aaatgaattt gaaacaatga aaattcggtg cgcacacctt
     1381 acagtgcagc ttgttgttaa acaaggcaga gatgtaaagg gatagtccct ttataattga
     1441 ggtggcaccg cgttaccaac gccctcacac ggaagtatat tctgtgtgtg ggcttttttc
     1501 tatccgtcgt ttggtttatc ttttattagg gccttaagtc gctttgatga acttgagttc
     1561 tatctacagc tccttgccta ctactaaaag caaacaaaaa ggctggacta atacgaaaaa
     1621 agctagtaat actcaatgaa aatcaaagag caaactaaga aactagccgc aggttgctca
     1681 aaacactgtt ttgaggttgt ggatagaact gacgaagtca gctcaaaata ctgttttgag
     1741 gttgt
//
LOCUS       DAVKWZ010000123         1653 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000123 DAVKWZ010000000
VERSION     DAVKWZ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.123"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(31..351)
                     /locus_tag="VW854_002128"
     CDS             complement(31..351)
                     /locus_tag="VW854_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV0263265.1"
                     /translation="MQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVRIGICRDDAKLIQKGFSLLELTEETSMLSHLKKEVEIYYQ
                     AKER"
     gene            1109..1480
                     /locus_tag="VW854_002129"
     CDS             1109..1480
                     /locus_tag="VW854_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0218 family bacteriocin-like peptide"
                     /protein_id="HEV0263266.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAIYIPRWAITGAITGAAYAAL
                     AAAGGGGLQLVLASYGLRSALVAGIVKGLGVLGIHIGNAFANTVIRSIASAGIGAGAD
                     WIFTNIIDGWDGRRDSQLRIG"
BASE COUNT          514 a          220 c          339 g          580 t
ORIGIN      
        1 gtaaagagtc gaggaaatcc ctcggctttt ttatctttcc ttcgcttggt aatagatctc
       61 tacttctttt ttgagatgag acagcataga agtttcctcg gtcagctcta ggagtgagaa
      121 ccctttttgg ataagtttcg catcatccct acaaatccca atgcggacat agcagatagc
      181 cagcctatcg tagcttttct tattcttggc gtcctccagt aaagtgtagc agatttgctt
      241 acacatgttt ttatcttgat tgtatagata gagtgtggaa aggttgagca ggattgctat
      301 tcgcaagtca tataaatgtt gatagttttt atagacttct aagcgttgca agattttctc
      361 ggattattga caaataaacg gacattctga tagacgattg ccctagcggc ttagtgtaaa
      421 ctttacaaat ttggcttgta atttttttat aaaattcact ttagttactt gacagttatt
      481 atattttcgc tcataatata gataaaagga tgggatagga gtttaaaaat ttgaaagcgc
      541 tatccataat cataacgcat aattttctcc ttttcgtgaa aaaagttaac gtaattttga
      601 aatgagctta ataagaggta ctatgtaggt agaaaataaa gataggagaa aaagtatgaa
      661 taaaaaaata ttttgtattc tagtatgtat tatatcattg attagtctag ctatagtctt
      721 tccatggggc tggtcgatat aatgtaaggg agcttgagtt gttagtggag gataaaatgt
      781 taagagtaat agtagttatt caatctttag taataatact gcttgctgtt gctttattat
      841 tatctcggaa aaactttgat tcgttaaatg ttgttcttat aatgatgatc gtatcacaat
      901 tgttgttatt agtatatttg ataaaatcag ggaatcagtg attagctgtt atttgaatta
      961 agtaaactgt taagcttcaa gcagtaactg gttttaaata tttaaaataa gataaaggaa
     1021 ggaagtctaa tttaggtaac taaacaaagc ctgcgatcac ttgttaggaa accatcctaa
     1081 ccatgcttaa taaaaaagga gaaaataaat ggaactcgta ttaccaaata attatgttgt
     1141 gattgatgaa gaagagatga tgtaccttga tgggggggct atatatatac ccaggtgggc
     1201 aattacagga gccattactg gtgcagcata tgcagcatta gcagcagcag gaggtggagg
     1261 ccttcaacta gttcttgcat cttatggatt acgctccgca ctggtagctg ggattgttaa
     1321 aggtttagga gtattaggaa ttcatattgg aaatgctttt gcaaatactg ttattagaag
     1381 tattgcatct gctggaattg gtgctggagc tgattggatt tttaccaata ttattgatgg
     1441 ctgggatggg cgacgtgata gtcaattgag aataggttaa taatcttatg ttggttgttt
     1501 taatttcagt tattgttgca ttcctgtatc ataaaattac agagtatgtc aacaacagga
     1561 aaattgatat tgttattttt ctatcattgc tgatttctca ttttatttgg aattactatt
     1621 ttttaccaat tgaaatattg atagcaatca gtg
//
LOCUS       DAVKWZ010000124         1593 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000124 DAVKWZ010000000
VERSION     DAVKWZ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.124"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            159..1505
                     /gene="gdhA"
                     /locus_tag="VW854_002130"
     CDS             159..1505
                     /gene="gdhA"
                     /locus_tag="VW854_002130"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEV0263267.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVISFRVPWVDRDGKVQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGSVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSNLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGVV"
BASE COUNT          493 a          314 c          329 g          457 t
ORIGIN      
        1 atttccaaga aaaacgttcg tttttttatc tattttcaaa aaaaaccaaa aactgaattg
       61 aaagaatttt tagaaaattt ctgttttttc tttacagtga aaaaaaattc tgctataatg
      121 tcttatgtaa taaaatatga caataaaagg agaacgatat gacatctgct aaagaatata
      181 tccaaagcgt gtttgaaact gtaaaagctc gtaacgggca cgaggctgaa ttcctccaag
      241 ctgttgaaga atttttcaac actttggaac ctgtatttga aaaacaccct gagtatatcg
      301 aagaaaatat cttggcacgt attactgagc ctgagcgcgt gatttctttc cgtgttcctt
      361 gggttgaccg tgatggaaaa gttcaagtaa accgcggcta ccgtgttcaa ttcaactcag
      421 ctgttggacc atacaaaggt ggacttcgtt tccacccaac tgtaaaccaa gggatcttga
      481 aattcctcgg atttgaacaa atctttaaaa atgtcttgac tggacttcct atcggtggag
      541 gtaaaggtgg atcagacttc gatcctaaag gtaaaacaga tgctgaagtg atgcgcttct
      601 gccaaagctt catgactgaa ttgcaaaaac acatcggacc atcacttgac gtacctgctg
      661 gtgatatcgg tgttggtgga cgtgaaattg gttaccttta cggtcaatac aaacgtctta
      721 accaatttga tgctggtgtc ttgactggta aacctcttgg atttggtggt agcttgattc
      781 gtccagaagc aactggttac ggtttggttt actatactga agaaatgctc aaagctaacg
      841 gtaacagctt tgctggtaag aaagtcgtta tttcaggttc tggtaacgtt gctcaatacg
      901 ctcttcaaaa agcaaccgaa cttggtgcaa cagttatctc tgtttctgac tcaaacggct
      961 atgttatcga tgaaaacggt atcgacttcg atcttttggt tgatgttaaa gaaaaacgtc
     1021 gcgctcgttt gactgagtat gcagctgaga aagcaactgc tacttatcat gaaggttctg
     1081 tatggactta cgctggaaac tatgacattg cgcttccatg tgctactcaa aacgaaatca
     1141 acggtgaagc agctaaacgt ttggttgctc aaggcgttat ctgtgtatct gaaggtgcca
     1201 acatgccaag caaccttgat gccatcaaag tttacaaaga aaatggtatc ttctacggac
     1261 ctgccaaagc tgccaacgct ggtggtgtag ccgtttcagc ccttgaaatg agccaaaaca
     1321 gccttcgcct ctcatggact cgtgaagaag ttgatggacg tctcaaagac atcatgacca
     1381 acatctttaa cacagctaaa acaacttcag aaacatatgg tcttgataaa gactaccttg
     1441 caggagctaa cattgctgcc tttgaaaatg tagcaaacgc tatgattgca caaggcgtgg
     1501 tataaaaaaa attacggaaa ctgactaccc agacgggtag ccagtttttt caattcctaa
     1561 aatttaagat tctaataccc tttatgcaaa aat
//
LOCUS       DAVKWZ010000125         1565 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000125 DAVKWZ010000000
VERSION     DAVKWZ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.125"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(<1..725)
                     /locus_tag="VW854_002131"
     CDS             complement(<1..725)
                     /locus_tag="VW854_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263268.1"
                     /translation="MKDDQKYLLAGLYSLLVAIFYFPLIESKGIFVSILMAVLLLYLI
                     YFIATVIHIVIIKFIRKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQ
                     EYCQGQPYGLLKKMLKRIRLSREISLLLATIIVYFFTHRILPLSVFTFIFSYILLFAQ
                     SYLGGNTAWIGNRRLIIDDEFEKILLSKSYIKEISSARYSEYLTCEYKNFTPIILLAI
                     FENLLDSYLLQNQSEVDLDIFYKV"
     gene            complement(882..1127)
                     /locus_tag="VW854_002132"
     CDS             complement(882..1127)
                     /locus_tag="VW854_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263269.1"
                     /translation="MGIYGRKTIAMKHFFAGIGEIHFLLFLLYLSMSASPIIGLVIGY
                     TKINLINSIIIALGMILALILVLSRVYRKYFYRDDDD"
BASE COUNT          592 a          278 c          192 g          503 t
ORIGIN      
        1 actttataaa aaatatctaa gtcaacctca ctctgattct gaagcaagta ggagtcaagc
       61 aaattttcaa aaatagctaa caatatgatt ggtgtaaagt ttttgtattc acatgtcaag
      121 tattctgagt atcgtgcaga tgatatttct tttatataac tttttgaaag caagattttt
      181 tcaaattcat catctattat taatcttcta tttccaatcc aagcggtatt accacccaaa
      241 taagattgtg caaatagcaa tatgtaagaa aatatgaacg taaatactga aagaggtaga
      301 attctatgcg taaaaaaata gacgataatg gtagccagga gtaaagatat ctctcgtgac
      361 aatcgaattc ttttcagcat cttttttaac agtccgtaag gttgtccttg gcaatattct
      421 tgaactaaat taatcggaat gacatctctc accatttctg gaaagtagag taagtttata
      481 ggttgaaatc tccagctccc atcataagta aatggatata gaaccaaata cttaaaagat
      541 ttttttctta taaacttaat tatcacaata tgaatgactg ttgcaatgaa gtatattaaa
      601 tataaaagta aaaccgccat caatatactt acaaaaatcc ccttggactc aatcaatgga
      661 aaataaaata ttgcaactag aagcgaataa agtcccgcta ataaatattt ctgatcatct
      721 ttcattcaaa acaccatatt atttactaag aatacatcaa tacaatagag caactctgta
      781 agtcaatatt tgataaagca aagctttaac cccttcccca gacgttagct tttcttcaac
      841 ccactacagt tgacaaggag cccaaattat tgataaaaac tttagtcatc atcgtctcta
      901 taaaaatatt ttctatacac tcttgataat accaaaatta atgccaatat cataccaaga
      961 gctataataa tagaatttat tagatttatt ttagtatatc caatcactag tccaataatt
     1021 ggactagcag acatgcttag atataataaa aataacaaaa aatgaatttc accaattcct
     1081 gcaaaaaaat gtttcatagc tatagtttta cgcccataaa tacccatcaa atttaccgtc
     1141 tacgatatct aaaccatatg caatggctcc tcctaatgag aagacttcag caacagtagc
     1201 cgcaatatta agagcattag taatagttgc catactaact ccaccaatat atcttgttat
     1261 agcacttagt tacctagata agatatcctt tagcttgctt ccaaaattta agtctacacc
     1321 tataatatga gttcatatta gtactgctaa atcctatcta tagtaatgtc aaacacctaa
     1381 atcaagtcat ctctccaatt catccaagaa atgaagaaag gcaaaaaatc tttaaaataa
     1441 gaaaagtgca tattatcagg tgttcaaaaa tcttgataat atgcgttata taatagaaaa
     1501 atttactcaa tttcattttc aaattgagtt ttttcccagc ctcattttta atttatatct
     1561 tcaat
//
LOCUS       DAVKWZ010000126         1456 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000126 DAVKWZ010000000
VERSION     DAVKWZ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.126"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            12..725
                     /locus_tag="VW854_002133"
     CDS             12..725
                     /locus_tag="VW854_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001840024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV0263270.1"
                     /translation="MHKKYSKNKLGFNNWIFSNYQITDEVKVLELGCGTGELWKSNSD
                     SIDKMKQLIVTDFSKDMVKSTKSVIGNRNNVNYEIMDIQKISFENETFDIVIANMLLH
                     HVNDIPKALSEVNRVLKTGGIFYCATFGENGVVNYLASLFKDEVNQDLENRTFTLQNG
                     KRYLSRYFNSVDTLLYDDELQVTSIDDLVKYIQSFKGISEIGSLEEEIIRKRLESEFN
                     NGMLIIPKEYGMFIARKES"
     gene            738..1076
                     /locus_tag="VW854_002134"
     CDS             738..1076
                     /locus_tag="VW854_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0263271.1"
                     /translation="MKNYGEAFRYFRKLNGYSLEYAAADSISKSQLSRFERGENEISL
                     STFFELLSNINVSIENFCNHLEHYKRSERDDFLVNLSPNFYSLNIKGLEVIKNKQQKL
                     FEKSGKKLIK"
     gene            1109..1456
                     /locus_tag="VW854_002135"
                     /pseudo
     CDS             1109..1456
                     /locus_tag="VW854_002135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01716.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator"
BASE COUNT          550 a          147 c          272 g          487 t
ORIGIN      
        1 ttcgagtaga attgcataag aagtattcaa aaaataaatt gggatttaat aattggatat
       61 tttctaacta ccaaatcact gatgaagtaa aggttctaga attgggatgt ggtacaggag
      121 aattgtggaa aagtaatagt gattctatag ataaaatgaa gcagttgatt gttacagatt
      181 tttctaaaga tatggtaaaa tcaacgaagt cagtgattgg aaatagaaac aatgtcaatt
      241 atgagataat ggatattcaa aagatttctt ttgagaatga aacttttgat attgttattg
      301 ctaatatgct tctacatcat gtgaatgata ttcctaaagc tctctctgag gtgaataggg
      361 tcttaaaaac cggaggaatt ttttactgtg ctacatttgg tgaaaatgga gttgtaaatt
      421 atttggcaag tttatttaaa gatgaggtta atcaagattt agaaaataga acgtttactt
      481 tacaaaatgg taaaaggtac ctgagtaggt attttaactc tgtcgataca ttactctatg
      541 atgatgagtt acaggtaact agtatagatg acctagttaa atacatccaa tcgtttaaag
      601 gaatttcaga aataggttca ctagaagagg aaataatacg taaaagattg gaaagtgagt
      661 ttaataatgg tatgttgatc attcctaaag aatatggtat gtttatcgct cgaaaagaaa
      721 gttaagggaa tgaaagtgtg aaaaattacg gagaagcttt tcgatatttt agaaaattaa
      781 acggatattc tttagagtat gctgcagctg attctatatc aaaatctcaa ctttctagat
      841 ttgaaagggg agaaaatgaa atttctcttt caacattttt tgaattatta tcaaatatca
      901 atgtttcgat agagaatttt tgtaatcact tagaacatta taaaagatcg gagcgtgatg
      961 attttttggt taatttatct ccaaattttt atagtttgaa tattaaagga ttggaggtaa
     1021 taaaaaataa gcaacaaaaa ctttttgaga agagtggaaa aaaactcata aaataaatac
     1081 aattatggtt cagggattat gaaatcagct agattgtaat catgtagtta gtagagatga
     1141 tctaaattta gtatatgact atttattcca gaaggaacga tgggagtcct atgagataac
     1201 gctgattggt aatttatatc atctttttga aatagattat atttacatgg tcggaaaaga
     1261 aatactagag cgtacacatt attacgaaaa aatcggcaag aatagaaatt tagttgtgtc
     1321 agcttgttta aatttttggt tctgttgcct tgaaaattca catctaatat atgcagacta
     1381 ctttgaaatg aaattacaaa aattattaaa agatgacaca aaagtttttg aaaaatctac
     1441 attcaaattt gtagaa
//
LOCUS       DAVKWZ010000127         1443 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000127 DAVKWZ010000000
VERSION     DAVKWZ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.127"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            1..632
                     /locus_tag="VW854_002136"
                     /pseudo
     CDS             1..632
                     /locus_tag="VW854_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            678..1124
                     /locus_tag="VW854_002137"
     CDS             678..1124
                     /locus_tag="VW854_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV0263272.1"
                     /translation="MNRSILDNITLKNEVTSQKIEEVCKAVQIYDEIMAMPMKFNTII
                     SEMGSNISGGQRQRIALARALINNPSIVILDEATSALDTINEERITKYIQSQGCTQII
                     IAHRLSTIKDADIIFVMKGGKIVESGNHKYLMDLGGEYYSLYTKRK"
     gene            1137..1307
                     /locus_tag="VW854_002138"
     CDS             1137..1307
                     /locus_tag="VW854_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263273.1"
                     /translation="MKKVNEYVISTAASLGVMIGIVFAIFLDFPVEYAISLGLLNGIV
                     LGSLIFYKNNKN"
BASE COUNT          543 a          158 c          270 g          472 t
ORIGIN      
        1 tttactcagt agaaacagaa gcattaaata ctatttcttc aataaaaata tcgggattag
       61 aagatgaaat atatgaaaac tggtctaagt atttaaaaaa cgtattgaca aagtataaaa
      121 aacgaagtat tgtgcatatt ttatacaatt cagctactaa tgtatttcaa ttgttcgctc
      181 ctataattat attgattttt ggacttgata atgttttaaa tggaaaaatt ttattgggtg
      241 aggttgtcgc atttcaaacc atggcatcaa ttttatttag ttcagaaatc tcaatattta
      301 atgcttatac gcagtatatt ttggcagcag gatacttaaa tagagtaaat gatatctggc
      361 ttgaaaatga agagaatgtt gaaaatggtc ttaaaaagtg ttctttagaa ggtagaatag
      421 atattaagga cctatctttc tcttattcca aagactcagc tcctgtaata gaaaatttaa
      481 atttgactat tgagccagga cagagaattg cactagtcgg tcaatcgggg tcaggaaaaa
      541 gtacgttatc aaaaatacta tcaggattat ataagattga tacaggaaaa attttatttg
      601 atggtgtaaa tattaatcaa atagataaaa aaatattaag tcaaaattta ggagtagttc
      661 cacaggattc atttttattg aaccgaagta ttcttgataa tataacttta aagaacgaag
      721 ttacttcaca aaagatagag gaagtttgta aagcagttca aatctatgat gaaatcatgg
      781 ctatgccgat gaaatttaat actatcatct cagagatggg gtcaaatatt tcaggtgggc
      841 aaaggcaacg gatagcactg gcacgtgcat taataaataa tcctagtatt gtaattttag
      901 atgaagcaac tagtgcatta gacactatta atgaggaaag aataacaaag tatatacaaa
      961 gtcagggttg tactcaaata attatagctc atagattgtc aacgattaag gatgcggata
     1021 ttatttttgt aatgaaaggt ggtaagattg ttgagtcagg aaatcataag tacttaatgg
     1081 atcttggtgg agagtactac agcttatata caaaaaggaa atgaggtgta aagaaaatga
     1141 agaaagtaaa tgaatatgta atttcaacag cagcctcact aggggtgatg attggaatag
     1201 tatttgcaat ttttttagat tttccagttg aatatgctat ttctttaggt ttgttgaatg
     1261 gaatagtatt gggttcgctg attttttaca aaaacaataa gaattaagca taattttttg
     1321 ctgtaaacta aggagtagag atggctatag ttgaaattat aaatctaaca aaaagcttta
     1381 aagatattga agttattcat aacacttcat tttatttaaa taagggaaaa gtatatggtt
     1441 ttg
//
LOCUS       DAVKWZ010000128         1387 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000128 DAVKWZ010000000
VERSION     DAVKWZ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.128"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            <1..444
                     /locus_tag="VW854_002139"
     CDS             <1..444
                     /locus_tag="VW854_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000921212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEV0263274.1"
                     /translation="VGKRDFNANMKSKLITFIQGLQNYNFVIQSCDFRKTMNQVNKGD
                     FVYTDPPYRITTAAYNENGAWTLKDDLDLFKYLDSINDKGAYFALSNVVIHNNKENKE
                     LMKWASKYNLHVLDYHYNNSNYQSKAKMSNTVEVLITNYNSKGDI"
     gene            441..>1387
                     /locus_tag="VW854_002140"
     CDS             441..>1387
                     /locus_tag="VW854_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlwI family type II restriction endonuclease"
                     /protein_id="HEV0263275.1"
                     /translation="MRLKESTSIFNMGDTSIRVKEVVPIYKQILKTLQKHNQSNQKWN
                     NESQASFYSSVLSDFAKAETEDGINLFGNLKRNEITGLDKRGRTLTNALVKIGFINYD
                     RKLSQVGLNYISETEQAFDKLEELFGLTIDNLVYFRQLLKLRIYDSNSDKYFYNFRFA
                     LAFLNRYSKVPVRDFLWIVESIKPNFSEEKIKVIIDNYQSVYDNNKTFEQYRDEEFAN
                     HILIPERVDEARKMFSIKNFSDENFKKLFPNRKNSNKSLEYKSFVLSIINFTEEKTER
                     SFEEMMSLSKQDAIKKAFGDGKAVFKYKNKDSIQDFITK"
BASE COUNT          522 a          164 c          239 g          462 t
ORIGIN      
        1 gtaggtaagc gtgattttaa cgctaatatg aaatctaaac taattacgtt cattcaaggt
       61 ttacaaaatt acaattttgt tatccaatct tgtgattttc gtaaaactat gaatcaagta
      121 aataaaggag attttgtata tacagatcct ccttatcgca ttactactgc cgcttacaat
      181 gaaaatggtg cgtggacatt aaaggatgat ttggatttgt ttaagtattt agatagcata
      241 aacgataagg gagcttattt tgctctttct aatgttgtca ttcataacaa taaagaaaat
      301 aaagaattga tgaaatgggc ttcaaagtat aatttacatg tattagatta ccattacaat
      361 aatagtaatt atcagtctaa ggcgaaaatg agtaatactg ttgaagtctt gattacaaac
      421 tataattcga aaggtgatat atgaggctaa aagagtcaac aagcattttt aatatgggtg
      481 atacgagtat tcgtgttaaa gaagttgtcc ctatctacaa acagatttta aaaactcttc
      541 aaaagcataa tcagtcaaat cagaaatgga acaatgagag tcaagcaagt ttttattcat
      601 cagttttaag cgattttgca aaagctgaaa cagaggatgg aattaacctt tttgggaatt
      661 tgaagagaaa tgaaataact ggtttagaca aaagagggag aactttaaca aatgctcttg
      721 tcaagattgg ttttattaac tatgatagaa aattatctca agttgggctg aattatattt
      781 ctgaaacaga gcaagctttt gataaattag aagaactttt tggtttaaca attgataatc
      841 ttgtttattt taggcaatta ttgaagcttc gtatatatga tagtaactct gataaatact
      901 tctataattt ccgttttgct cttgcttttt tgaacagata tagcaaagtt cctgttagag
      961 attttctatg gatagtggaa agcattaagc caaacttttc agaagaaaaa atcaaagtga
     1021 ttatcgataa ttaccaatct gtttatgata acaataaaac ttttgaacag tatagagatg
     1081 aagagttcgc taatcatata ttgattcctg aaagagtgga tgaagctcgc aaaatgttta
     1141 gtattaaaaa tttttcggat gaaaacttca aaaaactttt tccaaatcga aagaatagta
     1201 ataaaagttt agaatataaa agttttgttc tttcaattat aaactttaca gaagaaaaga
     1261 cagagcggtc atttgaagaa atgatgagtc tttctaaaca agatgctatt aagaaagctt
     1321 ttggagatgg taaggctgtt ttcaagtata aaaataaaga ttctattcaa gattttataa
     1381 ctaaaaa
//
LOCUS       DAVKWZ010000129         1195 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000129 DAVKWZ010000000
VERSION     DAVKWZ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.129"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(<1..530)
                     /locus_tag="VW854_002141"
     CDS             complement(<1..530)
                     /locus_tag="VW854_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263276.1"
                     /translation="METLQTIETKIDKLIEQNKKAIEMTEAELVKASQAISDAQAKLV
                     QAQEEINSEKYVEAKSDLWTAERTKEFHEGRLKELVTTPIISYDEYHATVKEIYRLAD
                     EQQNTFFTPAVAKLLEIVKLGDESAKEVGKVNEIFRKLEKDISKNNEDYKRDKNGGWF
                     RGPFSSLSYSPRNALHG"
     gene            complement(684..848)
                     /locus_tag="VW854_002142"
     CDS             complement(684..848)
                     /locus_tag="VW854_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HEV0263277.1"
                     /translation="MNPNKDYTPFLQDNFIIFNKNGIIEMKKIPDFGSVVFTSQDGNI
                     VQIETTIKER"
BASE COUNT          298 a          247 c          175 g          475 t
ORIGIN      
        1 ccatgtaagg cgtttcttgg agaataggat aaacttgaaa atggcccacg aaaccatcca
       61 ccgtttttgt cccttttata atcttcgtta tttttagaga tatctttttc aagttttcta
      121 aatatctcgt ttactttacc aacttctttg gcggactcat cacccagctt tacaatttcc
      181 aatagtttgg ctactgctgg agtaaagaaa gtattttgtt gttcatcagc caatctataa
      241 atctctttaa ccgttgcgtg atattcatca tatgaaatta ttggagttgt aactaattct
      301 ttcaaacgtc cctcatgaaa ttcctttgta cgttctgccg tccataggtc actttttgcc
      361 tcgacgtatt tttcagagtt tatttcttct tgagcctgta ctagcttggc ttgggcgtct
      421 gaaatagctt ggcttgcttt aaccagttcc gcctcagtca tctcaatagc tttcttattc
      481 tgttcaatca gtttgtcaat tttagtttca atagtttgta atgtttccat gttcttttat
      541 tcctcatttc ttttgattgt gcttattggt ttcaactctt tactgggtct ttattcctca
      601 ttacttcctc tttctcaaaa caaaaagaga cgcaacaaaa aagctactta gctttaatgt
      661 tacgcctcta gttgtctagt cagctatctt tctttaattg ttgtttcaat ctggacaatg
      721 ttgccatctt gactagtaaa taccacgcta ccaaagtctg gtattttctt catctctatt
      781 ataccatttt tgttaaagat aataaagtta tcctgtagaa atggagtgta atctttgttt
      841 ggattcatct gtttttcctc tcttttttgg tgccatatat ttaacatatc ttctattctg
      901 ttaaatttaa gcgacctccc ttctcgcctt gtctatcaag ggaggagggc tatttttgtg
      961 atttcctatt tacattttct cattatgtaa aatacaaata tgatttcagt cttaaaagtt
     1021 actcatttaa gttactattt ccatttcttt ttaggtctcc tcctcacaat tccaaaaagt
     1081 gtagtcattt taggatatct agtgactacg ctctccgcct tacagtcaca aggcttttcg
     1141 ccatgcgtag tcatgtagtc accttgctcc caaaaaaata aacaataaac gcctt
//
LOCUS       DAVKWZ010000130         1175 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000130 DAVKWZ010000000
VERSION     DAVKWZ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.130"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(<1..249)
                     /locus_tag="VW854_002143"
     CDS             complement(<1..249)
                     /locus_tag="VW854_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263278.1"
                     /translation="MSNVDKIRKIHIIVCWVYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLMSFPILINKFFPESF"
     gene            complement(246..599)
                     /locus_tag="VW854_002144"
     CDS             complement(246..599)
                     /locus_tag="VW854_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEV0263279.1"
                     /translation="MELVLPNNYVVIDEEEMMYFDGGAYLSKRACQGICAALAMSSGT
                     FIALAGAAVLTKKLINYIKVGGLGGWLIGAAAGVLATAAGKIAYYIGYGVLNRGCDIN
                     GNPYPWDGFISATVR"
     gene            complement(655..>1175)
                     /locus_tag="VW854_002145"
     CDS             complement(655..>1175)
                     /locus_tag="VW854_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263280.1"
                     /translation="FAIIMLTTIFFWTIMDINHLVFNKTEFLFNQNKWQKEDSFESDL
                     TKTLKDKIQKSELSYSDLMSLQLYDAMNQSTFLTDSELFEDILKKIEDSHNTLLCTGL
                     VELLLYEISISNNNNWQEKVDKIRIQLIRINQLDFFYYTSWLRQNFDFCMNREYHKMK
                     SRKLLLSNKKIV"
BASE COUNT          400 a          219 c          158 g          398 t
ORIGIN      
        1 aaaactttct gggaaaaact tattgattaa aatggggaaa ctcattaaca gaatcgaatt
       61 tatggctaga cagatagata ctattttatt tttcacagaa tatagaggta gtgatactat
      121 tgagtagata aaagctaaaa aaataagcaa aaaatactcc gtatcattta taatcgccct
      181 gaatgataaa aatatataca cccaacacac gatgatgtgt atttttcgaa tcttatctac
      241 attactcatc ttactgttgc agatatgaat ccatcccaag gataagggtt cccgttaata
      301 tcacaacctc tattaagaac accatatcca atatagtaag ctattttccc tgctgctgta
      361 gccaatacac ctgctgctgc accaataagc cagcctccaa gacctccaac cttaatatag
      421 tttattagtt ttttggttaa aactgcagct ccagctaatg ctataaaagt tcctgaactc
      481 atagctaaag ctgcgcaaat tccttgacaa gcacgcttgc ttaaataagc tcccccatca
      541 aaatacatca tttcttcttc atcaatcaca acataattat ttggtaatac aagttccatt
      601 tattttctcc tccttagttg aaattgtttc atttgtttta caaataggac gaacttaaac
      661 tatcttttta ttactcagta ataatttccg agatttcatt ttatgatact ctctattcat
      721 acagaaatca aaattttgcc ttaaccaact tgtataatag aaaaaatcta attgattgat
      781 tctgatgagc tgtattctaa ttttatcaac cttttcttgc cagttgttat tgttactaat
      841 agaaatttca tacagcagta actcgacaag acctgtacac agaagggtgt tatgagaatc
      901 ttcgattttt ttcaaaatat cctcaaaaag ttcactatcc gttaaaaagg tggattggtt
      961 catcgcatca tacagctgaa gagacattaa gtcagaataa cttagctctg atttttgtat
     1021 cttatccttt aacgttttcg tcaaatcact ttcaaacgaa tcttcttttt gccatttgtt
     1081 ttgattaaaa agaaattccg tcttattaaa aacgagatga ttgatatcca ttatagtcca
     1141 gaaaaatatt gtagtaagca tgatgattgc aaaag
//
LOCUS       DAVKWZ010000131         1118 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000131 DAVKWZ010000000
VERSION     DAVKWZ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.131"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            60..>1118
                     /gene="pcpA"
                     /locus_tag="VW854_002146"
     CDS             60..>1118
                     /gene="pcpA"
                     /locus_tag="VW854_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HEV0263281.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGNLELKELILPDNVKN"
BASE COUNT          438 a          157 c          180 g          343 t
ORIGIN      
        1 acggctagca aagttataat tttatatcta ttagaaaata aatatgtaag gaaatttaaa
       61 tgaaaaaaac tacaatatta tcattaacta cagctgcggt tattttagca gcatatgtcc
      121 ctaatgaacc aatcctagca gatactccta gttcggaagt aatcaaagag actaaagttg
      181 gaagtattat tcaacaaaat aatatcaaat ataaggttct aactgtagaa ggtaacatag
      241 gaactgttca agtgggtaat ggagttactc ctgtagagtt tgaagctggt caagatggaa
      301 aaccattcac gattcctaca aaaatcacag taggtgataa agtatttacc gttactgaag
      361 tagctagtca agcttttagt tattatccag atgaaacagg tagaattgtc tactatccta
      421 gctctattac tatcccatca agcataaaaa aaatacaaaa aaaaggcttc catggaagta
      481 aagctaaaac tattattttt gacaaaggca gtcagctgga gaaaattgaa gatagagctt
      541 ttgatttttc tgaattagaa gagattgaat tgcctgcatc tctagaatat attggaacaa
      601 gtgcattttc ttttagtcaa aaattgaaaa agctaacctt ttcctcaagt tcaaaattag
      661 aattaatatc acatgaggct tttgctaatt tatcaaattt agagaaacta acattaccaa
      721 aatcggttaa aacattagga agtaatctat ttagactcac tactagctta aaacatgttg
      781 atgttgaaga aggaaatgaa tcgtttgcct cagttgatgg tgttttgttt tcaaaagata
      841 aaacccaatt aatttattat ccaagtcaaa aaaatgacga aagttataaa acgcctaagg
      901 agacaaaaga acttgcatca tattcattta ataaaaattc ttacttgaaa aaactcgaat
      961 tgaatgaagg tttagaaaaa atcggtactt ttgcatttgc agatgcgatt aaacttgaag
     1021 aaattagctt accaaatagt ttagaaacta ttgaacgttt agccttttac ggtaatttag
     1081 aattaaaaga acttatatta ccagataatg ttaaaaat
//
LOCUS       DAVKWZ010000132         1104 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000132 DAVKWZ010000000
VERSION     DAVKWZ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.132"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            207..882
                     /locus_tag="VW854_002147"
                     /pseudo
     CDS             207..882
                     /locus_tag="VW854_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921814.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          376 a          181 c          204 g          343 t
ORIGIN      
        1 agagtatctc tttattacag aatgctataa actccaaaaa acttgatttt ggagtagtta
       61 taaaaactca ttcagataat ttattatttg attttgatac gattcctgaa aatataatag
      121 tagttgacca ttttgaactt acaatcgaaa aatggttaag taaaagttaa cagttactat
      181 agtttgcaaa ttatttacag aaagaacaag aattcctagg agattattct ggctatgtgc
      241 attgtgatat gttgcggcag taacttagga ctttagtcct ctagttctgc ctatgcgata
      301 gcagtccaag gtttaggagc aaggcgacgc taagcttggt aaactacgaa ccgcagcaag
      361 cggataaatc atccttagga gctaaaggtt tagcttattg tgatcagtta tttgccttgg
      421 aaagagactg ggaggacatt gtcagctgat gaacgactac agaaacgtca agaagagctc
      481 caacccctaa tggaagactt ctttgcttgg tgccgtcgtc agtcagtttt agcaggttca
      541 aaactaggaa gggcaattga atacagcctc aagtataaag aaacctttaa gactatttta
      601 aaagacggac atctggtcct ttccaataat ctagctgaac gcgccattaa atcattggtt
      661 atgggacgta aaaattggtt gttttctcaa agttttgaag gagctaaagc aagagctatt
      721 attatgaact tattggaaac agctaaacgt catcaactaa atagtgagaa atatctatcc
      781 tatcttctag aatgtcttcc aaacgaggaa actctcgtaa acaaagaggt tttagaggct
      841 tatttaccat ggactaaagt tgtacaagaa aagtgcaaat aagaaatctc cagattagga
      901 actatccgtg agttctctag tctggagatt tttcaataga cttcgttatt gggcggttac
      961 aatttattat atgaaaatcc catatcattc tccaattcta tattttacct ttctaaatgt
     1021 atagattaac tacctaatta tagcatataa cgcagattcc tttcaatcgt atgatttact
     1081 gcattaaatt aagtaaaaaa ataa
//
LOCUS       DAVKWZ010000133         1010 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000133 DAVKWZ010000000
VERSION     DAVKWZ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.133"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            <1..357
                     /locus_tag="VW854_002148"
     CDS             <1..357
                     /locus_tag="VW854_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263282.1"
                     /translation="RKEIFDKTNIGNNDLSIIENVLTNVEKIRELNLCHGSVGTISCL
                     DAILKDEENLLIKESIDFYFDNVVSQVIKPELSTDLNTMNTFSFMLGVSGVVYEISRK
                     QDDRLLNVLLLELKRT"
     gene            357..>1010
                     /locus_tag="VW854_002149"
     CDS             357..>1010
                     /locus_tag="VW854_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0263283.1"
                     /translation="MTGKVPNIEQMSQIECGLCCCLSILHFYKSKETLLDLRRDIEKG
                     RDGYSIGDLKQLLNKRNFDTGSYQVKDVNKISELPLPLIAFWDNQHYVVIYKVKKNKV
                     YIMDPSKGYINYEFKEFSKHFSNIVLLSFPNENYQSLKSQFPSPWIRVFSSFSKVKGR
                     LILTLLFSIISYLIILSVPVMTSKFINSALGNTFSFQTSFLILFSLLCLYLISILARS
                     "
BASE COUNT          360 a          118 c          167 g          365 t
ORIGIN      
        1 agaaaagaaa ttttcgataa aactaatatt gggaataatg atttatcaat aattgaaaat
       61 gtattaacta atgtagaaaa aataagagaa ttgaatttat gtcacggttc tgtaggtact
      121 atcagttgtc ttgatgcaat attgaaagat gaagaaaacc tattaataaa agaatctata
      181 gatttttatt ttgataatgt cgtttctcag gttatcaaac cagaattaag tacggatttg
      241 aatactatga atacattttc ttttatgctg ggagtatctg gagttgtcta tgaaatatct
      301 cgaaaacaag atgatagatt acttaatgtt ttattactgg agttaaagag gacatgatga
      361 ctggaaaagt acctaatatt gagcaaatgt ctcaaataga atgtggttta tgttgctgct
      421 tgtccatact acatttctat aaaagcaaag agacattact agatttaaga cgtgatatag
      481 aaaaagggag agatggttat agtataggtg acttgaaaca gttattgaat aaacgaaatt
      541 ttgatactgg ctcatatcaa gttaaagatg taaataaaat tagtgaactt ccgttaccat
      601 taatagcatt ctgggataat caacattatg tagttattta caaagtaaaa aagaataagg
      661 tctatataat ggatccttct aaaggatata taaattatga gtttaaggag ttttcaaaac
      721 attttagtaa tatagtactg ctatcatttc ctaacgaaaa ttatcaatct ttgaagtcgc
      781 agtttccttc tccatggata agagtattct ctagtttttc aaaggtaaaa ggtcgattga
      841 ttttgacgtt actattttca ataataagtt acttaataat actttccgta ccagtaatga
      901 cttcaaagtt tattaattct gcattaggga atacattttc ttttcaaacc tcgtttttaa
      961 ttttattttc tctactttgt ttgtatctta tttctatctt ggctaggagt
//
LOCUS       DAVKWZ010000134          974 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000134 DAVKWZ010000000
VERSION     DAVKWZ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.134"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            <1..611
                     /locus_tag="VW854_002150"
     CDS             <1..611
                     /locus_tag="VW854_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0263284.1"
                     /translation="IRPFIENFFIEGIDFNNLHKYENIDTVVVHEYLSQILLFDYDDP
                     TQAIKDRLKQYRIEYSDIVPQDMNDTELFQFIVYGYFSQAMDIFLTSYSFNISPFFSS
                     LTTYINYNVFLLNIVHNSKKEKLEHFLKMSRLTLPIWFEYDKKGVELSISEIRERAKN
                     INFSDRIREIYNVLDDFNTKEQLITEVENCVHLLINYEGRGC"
BASE COUNT          346 a          150 c          150 g          328 t
ORIGIN      
        1 atatcagacc gtttattgaa aacttcttta tagaaggcat tgattttaat aatttacaca
       61 agtacgagaa tatagatact gtggtcgtac atgaatactt atcgcagata ttactctttg
      121 attatgatga tcctacacaa gcgataaaag accgtttaaa acaatatcga attgagtact
      181 cagatatagt cccacaagat atgaatgata cagaactttt ccaatttatt gtctatggct
      241 atttcagtca agctatggat atttttctga cttcctactc tttcaatatt tccccattct
      301 tttctagctt aactacatac ataaattata atgttttctt attaaatatc gtgcacaatt
      361 cgaaaaaaga aaaactagaa catttcttaa aaatgtcaag attaacctta cccatttggt
      421 ttgaatacga taaaaaagga gttgagctaa gtatttctga aatacgtgaa agagcaaaaa
      481 atataaactt ttcagatcgt ataagagaga tttataatgt tctcgatgat ttcaacacca
      541 aagagcagtt gattactgag gttgagaatt gtgtacattt attgataaat tatgaaggta
      601 gaggttgcta gtaatgttgt ttctcaataa cataagctcc gaaaactaaa aaaatgataa
      661 ccctttgata acctctcgat ttttaaaagg gttatatatg taaagatcga ggatttatct
      721 cttaaaatta cttgatttta gtgataattt accataaata tcataatgta gaaaaaacca
      781 ggtcttaaga aagctcgtaa agcatcacaa tttagtaaac gttaattcga aagaattact
      841 atacttatac agagcacctt tcggggtgtt cttttttata ctttcttact aaattggtgc
      901 aattgacaca gttgttgcga ctttagtcgc ttacaaatgt ggctgcaacc tgacaaggtc
      961 agttgcctca aaac
//
LOCUS       DAVKWZ010000135          918 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000135 DAVKWZ010000000
VERSION     DAVKWZ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.135"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(117..833)
                     /locus_tag="VW854_002151"
     CDS             complement(117..833)
                     /locus_tag="VW854_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV0263285.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
BASE COUNT          273 a          144 c          180 g          321 t
ORIGIN      
        1 atttctataa aatattccac aaattataga attttccaaa aataggtaag cgctaccttt
       61 ttggtgtagt ataataagca tagaaaaagc ccaagcgatt agctcaggtt ttcttcttag
      121 tgatcacggt cacatgagat aaatttaatc ttgtagtaat cagatcgttt gtaagtttca
      181 ctgtattcta aaacttggcc agttgattcg agtttggtga ttttagtttg taggacagta
      241 gggaattgtt catcgactcc gaggactgaa gctgcatgtt ctggagttgg aaagactatt
      301 tcgttgattt cttcaaagtg ttcatcattc atgtgaatgt ggtagtctaa cttgaaacga
      361 ttatagatag aactatagta ttcaaggttt ggataatttg cgttgatata ttgttctggg
      421 atgtaggatg tatggtagat ataaacgaca ccgtttgatt cgcggatacg ttcaatcttg
      481 tagtagaatt gatcgccgcg tagacccaat ttttccaagt aaacaagctt gtttccgcgt
      541 tcaattgaaa gaacagttac cttatcatct ttagcattga agagttcaat atctgaaaac
      601 tctacaagct tgtgtttgcg tgcacgtgaa acgaaggttc cttttccttg ttggcggaca
      661 atatagccat ctttggcaag gtcgtttaag gcgcgaacaa ctgtgataga gctgacatcg
      721 tacattgaaa tgagttctgc ttcagtgtaa aatttatctc cactgctaaa ctgcccagag
      781 atgattttat tttttaattc gtcttttatg tattgatatt tgggaattgc catattttta
      841 cctctatttt ccttcttcca tagaaaattt taccataaaa gcgaaaaaaa tagtagtctt
      901 ttgaataaaa aattagaa
//
LOCUS       DAVKWZ010000136          877 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000136 DAVKWZ010000000
VERSION     DAVKWZ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.136"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(<1..>877)
                     /locus_tag="VW854_002152"
     CDS             complement(<1..>877)
                     /locus_tag="VW854_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4135 domain-containing protein"
                     /protein_id="HEV0263286.1"
                     /translation="EVHVVLPSSNVKKETNKFYGKIIDGFKACYTILENSKNYFIEEI
                     KLLSEKYPLYLRQVLRPTMVYVKFLEASTHPDYLTDIKKQRKLFQKLNSSLYKLDSTL
                     GKQVEIEINSLMKGDVPYFYTKFNSLSIYSMENKICNFYATSIQSKLLERFQKLGNKD
                     MLKQLHVIFLSLSTVKDTTFSDINQSPLHDFIIPVNDENSLVLKVGDYVNHQVVWNNN
                     NEFCILPSVTQIDDKKVILPVSYCLYDGGGVVLLLYYLYLKFNEEKYKIVADGLLKGF
                     ESMIEIETNRNFSAFN"
BASE COUNT          301 a          144 c          108 g          324 t
ORIGIN      
        1 attaaacgcc gaaaagtttc tatttgtttc aatttcaatc attgactcga acccctttag
       61 taaaccatcc gcaactatct tatacttttc ttcgttgaat tttaaatata gataatataa
      121 tagtaaaact actccaccac catcatacag gcaataagaa actggtaaaa taactttctt
      181 atcatcaatc tgagtaacag aaggaagtat gcaaaactca ttattattat tccaaacgac
      241 ttgatgatta acatagtcgc cgacctttaa tactagagaa ttttcgtcat taacaggaat
      301 aataaaatcg tgtaatgggc tttgatttat gtcagagaaa gtcgtatctt taactgtcga
      361 taaagataaa aaaatcacat gcaactgctt taacatatcc ttatttccca gtttttgaaa
      421 tctttcaagt agtttacttt ggatagatgt agcataaaaa ttacaaattt tattttccat
      481 agaataaata ctcagactat taaattttgt ataaaagtac ggtacatctc ctttcattaa
      541 actattaatt tcaatttcta cttgcttgcc taaagtactg tctaatttat ataacgagct
      601 attaagtttt tgaaatagct ttctctgttt tttgatatcc gtaaggtaat ctgggtgggt
      661 tgaagcttct aaaaacttaa catataccat agttggtctt agaacctgtc tcagatatag
      721 aggatatttt tctgacaata attttatttc ttcaataaaa taattcttac tattttcaag
      781 tatagtataa caagctttga atccatcaat tatcttacca taaaatttat tagtttcttt
      841 tttgacatta ctactcggaa gtactacatg tacttct
//
LOCUS       DAVKWZ010000137          843 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000137 DAVKWZ010000000
VERSION     DAVKWZ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.137"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            <1..>843
                     /gene="phtD"
                     /locus_tag="VW854_002153"
     CDS             <1..>843
                     /gene="phtD"
                     /locus_tag="VW854_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtD"
                     /protein_id="HEV0263287.1"
                     /translation="AQAYWNGKQGSRPSSSSSHNANPAQPRLSENHNLTVTPTYHQNQ
                     GENISSLLRELYAKPLSERHVESDGLIFDPAQITSRTANGVAVPHGDHYHFIPYSQLS
                     PLEEKLARIIPLRYRSNHWVPDSRPEQPSPQSTPEPSPSPQPAPNPQPAPSNPIDEKL
                     VKEAVRKVGDGYVFEENGVSRYIPAKDLSAETAAGIDSKLAKQESLSHKLGAKKTDLP
                     SSDREFYNKAYDLLARIHQDLLDNKGRQVDFEALDNLLERLKDVSSDKVKLVDDILAF
                     LAPIR"
BASE COUNT          262 a          194 c          165 g          222 t
ORIGIN      
        1 gcccaagcct attggaatgg gaagcaggga tctcgtcctt cttcaagttc tagtcataat
       61 gcaaatccag ctcaaccaag attgtcagag aaccacaatc tgactgtcac tccaacttat
      121 catcaaaatc aaggggaaaa catttcaagc cttttacgtg aattgtatgc taaaccctta
      181 tcagaacgcc atgtagaatc tgatggcctt attttcgacc cagcgcaaat cacaagtcga
      241 accgccaatg gtgttgctgt accgcacgga gaccattatc actttattcc ttattcacaa
      301 ctgtcacctt tggaagaaaa attggctcgt attattcccc ttcgttatcg ttcaaaccat
      361 tgggtaccag attcaagacc agaacaacca agtccacaat cgactccgga acctagtcca
      421 agtccgcaac ctgcaccaaa tcctcaacca gctccaagca atccaattga tgagaaattg
      481 gtcaaagaag ctgttcgaaa agtaggcgat ggttatgtct ttgaggagaa tggagtttct
      541 cgttatatcc cagccaagga tctttcagca gaaacagcag caggcattga tagcaaactg
      601 gccaagcagg aaagtttatc tcataagcta ggagctaaga aaactgacct cccatctagt
      661 gatcgagaat tttacaataa ggcttatgac ttactagcaa gaattcacca agatttactt
      721 gataataaag gtcgacaagt tgattttgag gctttggata acctgttgga acgactcaag
      781 gatgtctcaa gtgataaagt caagttagtg gatgatattc ttgccttctt agctccgatt
      841 cgt
//
LOCUS       DAVKWZ010000138          771 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000138 DAVKWZ010000000
VERSION     DAVKWZ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.138"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(<1..587)
                     /locus_tag="VW854_002154"
     CDS             complement(<1..587)
                     /locus_tag="VW854_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEV0263288.1"
                     /translation="MSNNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPYVSDSSMVSYMGKTKELKMYQE"
BASE COUNT          265 a          152 c           85 g          269 t
ORIGIN      
        1 tcctcttgat acatttttaa ttctttagtt tttcccatat aactaaccat actactatca
       61 cttacatatg ggaagtcctc ataatatatt actttataac gcataaattc aagcgccctt
      121 ccaatactat tcacaaaaac atgatcaaca tggtcaccaa gtgaaagcgg acaatatacg
      181 acacatttgt cgtctaaatg cattaacagc tcttttatga tatcattctt taatgtgtcc
      241 tcatttttta attcactata gatatgacgg tatagaaaat tgccatttct atctttccta
      301 tagagacatt catagtacga taagtgtcta aaatcacatt gtagacgttc acaagctaac
      361 ctgtcttctt tcttcctttc ttcaatcgga tatttcccaa ggttacacaa cttatgaaat
      421 tgcttagcag agggctgtag ctgttggctc aaagggtaac cagaaaatat agtaataaca
      481 agtacaattt ctccttctga agttaatttt gaaatataat caccacagga aaaaattgcg
      541 tcatctaaat gtggagataa aaagatatac ttagtattgt tactcataac cattccctct
      601 acaatttatc taaaaactca ctaagtgtct gattaaattc cacatcatca aaaaaattca
      661 ccttattctt aataatgaat atttcgttaa ataaacatat atataaatat ttcaatatcc
      721 tttcaatatc atcctctaaa ttctcctcaa tattttgtat cagcccattt a
//
LOCUS       DAVKWZ010000139          652 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000139 DAVKWZ010000000
VERSION     DAVKWZ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.139"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            <1..615
                     /locus_tag="VW854_002155"
     CDS             <1..615
                     /locus_tag="VW854_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV0263289.1"
                     /translation="YLKEGGEYQIKNLSYKFTDDECLSLKDISFKLGKIYGIIGSNGR
                     GKSTLLRCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVMQDVNHQLFTDEVFNELRL
                     GIKNFDEEKAKIILKDLGLDEFIERHPMSLSGGQKQRLAIASVMCKTSPFIFFDEPSS
                     GMDYSNMIKISELINKYKTMDKIIFIVSHDIEFLNEVADEIFEL"
BASE COUNT          256 a           74 c          122 g          200 t
ORIGIN      
        1 tatttaaaag aaggtggaga gtatcagata aaaaatctta gctacaaatt tactgatgat
       61 gagtgtttaa gcttaaaaga tatttcattc aagcttggga aaatttatgg cataatagga
      121 tccaacggac gaggaaaatc aacgctttta agatgtttaa taggtcttga gaaaaaatca
      181 aaagaagaaa tttattttaa gggagagaag ctatctaaaa aagaaagact caaaaactct
      241 tcacttgtta tgcaagatgt aaatcatcaa ttattcacag atgaggtatt caacgagctt
      301 agattaggaa taaagaattt tgatgaagaa aaggcgaaaa tcattttaaa agatttaggc
      361 ctggacgaat ttattgaaag gcatccgatg agtttatcag gagggcaaaa gcaaaggctt
      421 gcaatagcat ctgttatgtg caagacttct ccatttatct tttttgacga accttcaagt
      481 ggtatggatt attccaatat gataaaaata tctgaactga ttaataagta taaaaccatg
      541 gataaaataa tttttattgt ttcccatgat atagaatttt taaatgaagt ggcagatgaa
      601 atttttgaat tataaaatag agtctgttaa acatgtctat agaactggta aa
//
LOCUS       DAVKWZ010000140          639 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000140 DAVKWZ010000000
VERSION     DAVKWZ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.140"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            2..>639
                     /locus_tag="VW854_002156"
     CDS             2..>639
                     /locus_tag="VW854_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV0263290.1"
                     /translation="MKSCLHQSYKNIEVIIIDDNYSNVNLRNKIIHQFGYTNHRIKLI
                     LSNEDLGATNARNIGIKNSRGKYISFLDDDDEYMPDRILKLMACFKKSRMKNLALVYS
                     YGIIIYPNGTREEEKTDFVGNPLFVQMVHNIAGTSFWLCKKEVLELINGFEKIDSHQD
                     GVVLLKLLAQGYQIDIVREFLVNYYAHSKENGITGVTQKTINADEEYYNYCR"
BASE COUNT          247 a           64 c          121 g          207 t
ORIGIN      
        1 tgtgaaaagt tgtttacatc aaagttataa aaatatcgaa gttataatta ttgatgataa
       61 ttatagtaat gtaaatttaa gaaataaaat tatccatcaa tttggctata ccaatcatag
      121 aattaagtta attttaagta atgaagattt aggtgcaact aatgccagaa acataggtat
      181 aaaaaattct agaggtaagt atatatcatt tttagacgat gatgatgaat atatgccaga
      241 tcgaatttta aagttgatgg cttgttttaa aaagagtaga atgaagaatt tagctttagt
      301 ttatagttat ggaataataa tttatccaaa tggtacacga gaagaggaga agaccgattt
      361 tgttggaaat cccttgtttg ttcaaatggt tcacaatata gcaggtacgt cattttggtt
      421 gtgtaaaaaa gaggtgctag aattaattaa tggttttgag aaaatagatt cacatcagga
      481 cggtgttgtt ttattaaaac tacttgctca aggataccaa attgatatag tgcgagaatt
      541 cttggtgaat tactacgctc acagtaaaga aaacggtatc actggagtga cacaaaaaac
      601 aattaatgca gatgaagaat attataatta ctgtaggaa
//
LOCUS       DAVKWZ010000141          636 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000141 DAVKWZ010000000
VERSION     DAVKWZ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.141"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            105..584
                     /locus_tag="VW854_002157"
     CDS             105..584
                     /locus_tag="VW854_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV0263291.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
BASE COUNT          238 a           82 c          147 g          169 t
ORIGIN      
        1 gcctctcttt tttgtgcagt ttaggagcta aagggaacag aatggagaaa atggaacaaa
       61 tgtgttttct aatctgttag actgtatcta gaaaggggaa aattatgatt aaagaattgt
      121 atgaagaagt ccaagggact gtgtataagt gtagaaatga atattacctt catttatggg
      181 aattgtcgga ttgggaccaa gaaggcatgc tctgcttaca tgaattgatt agtagagaag
      241 aaggactggt agacgatatt ccacgtttaa ggaaatattt caaaaccaag tttcgaaatc
      301 gaattttaga ctatatccgt aagcaggaaa gtcagaagcg tagatacgat aaagaaccct
      361 atgaagaagt gggtgagatc agtcatcgta taagtgaggg gggtctctgg ctagatgatt
      421 attatctctt tcatgaaaca ctaagagatt atagaaacaa acaaagtaaa gagaaacaag
      481 aagaactaga acgcgtctta agcaatgaac gatttcgagg gcgtcaaaga gtattaagag
      541 acttacgcat tgtgtttaag gagtttacta tccgtaccca ctagtaagtc atgcaaaaaa
      601 aatgaaaaaa attagaaaaa gtagttgaca aagttt
//
LOCUS       DAVKWZ010000142          608 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000142 DAVKWZ010000000
VERSION     DAVKWZ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.142"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            <1..>608
                     /locus_tag="VW854_002158"
     CDS             <1..>608
                     /locus_tag="VW854_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV0263292.1"
                     /translation="ALSVCSYELGRYQAGQDKKESNRVAYIDGDQAGQKAENLTPDEV
                     SKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISEELLMKDPNYQLKDSD
                     IVNEIKGGYVIKVNGKYYVYLKDAAHADNIRTKEEIKRQKQERSHNHNSRADNAVAAA
                     RAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKSD"
BASE COUNT          196 a          110 c          141 g          161 t
ORIGIN      
        1 gccctaagtg tttgttccta tgagcttgga cgttaccaag ctggtcagga taagaaagag
       61 tctaatcgag ttgcttatat agatggtgat caggctggtc aaaaggcaga aaacttgaca
      121 ccagatgaag tcagtaagag ggaggggatc aacgccgaac aaattgttat caagattacg
      181 gatcaaggtt atgtgacctc tcatggagac cattatcatt actataatgg caaggttcct
      241 tatgatgcca tcatcagtga agagctcctc atgaaagatc cgaattatca gttgaaggat
      301 tcagacattg tcaatgaaat caagggtggt tatgtcatta aggtaaacgg taaatactat
      361 gtttacctta aggatgcagc tcatgcggat aatattcgga caaaagaaga gattaaacgt
      421 cagaagcagg aacgcagtca taatcacaac tcaagagcag ataatgctgt tgctgcagcc
      481 agagcccaag gacgttatac aacggatgat gggtatatct tcaatgcttc tgatatcatt
      541 gaagatactg gtgatgctta tatcgttcct cacggcgacc attaccatta cattcctaag
      601 agtgattt
//
LOCUS       DAVKWZ010000143          595 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000143 DAVKWZ010000000
VERSION     DAVKWZ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.143"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            complement(<1..>595)
                     /locus_tag="VW854_002159"
     CDS             complement(<1..>595)
                     /locus_tag="VW854_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4135 domain-containing protein"
                     /protein_id="HEV0263293.1"
                     /translation="FLSSGNTIIYKPRSLNIEKSFEKMMEFLRDQNAILDYKLPNIIS
                     TEKYGFCEYIDTEECMNDLDVKGFYQRIGELLGILYSLNSVDFHYENIIAKGAYPVPI
                     DLETLIHPQIVDRNNDLSAFKKASKKFESSVITTGLLPIFLKGNEVGGVSMNSEQIST
                     FKTDYIKDTNSDNIHIEREYYVISPKNNNPIINGKIVD"
BASE COUNT          198 a           99 c           72 g          226 t
ORIGIN      
        1 atctacaatc ttaccattga taataggatt gttgttttta ggagaaatga cataatactc
       61 tctctcaata tgaatattat cgctgttagt atccttgata tagtcagttt taaaggttga
      121 tatttgctca ctattcatac taacaccacc aacttcattt ccttttaaaa aaatgggtaa
      181 aagccctgta gttattactg agctttcaaa ttttttagaa gcttttttga atgctgataa
      241 atcattattt ctatccacta tctgaggatg gattaatgtt tctaaatcaa ttggaactgg
      301 ataagctcct ttcgcaataa tgttttcata atgaaaatct acagaattca aagaataaag
      361 tattcccaac aattctccta ttctttgata aaaccccttt acatccaaat cattcataca
      421 ttcttctgta tctatatatt cacaaaatcc atatttttca gttgaaataa tattaggtag
      481 tttataatct aaaatcgcat tttgatctcg taagaattcc atcatttttt caaacgattt
      541 ttcaatatta agcgagcgag gcttatatat tatagtgttt ccgcttgata aaaaa
//
LOCUS       DAVKWZ010000144          534 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000144 DAVKWZ010000000
VERSION     DAVKWZ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.144"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            <1..>534
                     /locus_tag="VW854_002160"
     CDS             <1..>534
                     /locus_tag="VW854_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV0263294.1"
                     /translation="YTKTNFINQKDKKGSIEDSVLNIVFVTTRTKYCSQILQLLLKYF
                     DKNPNKISEVYSILEANYGLVTEREYIDYTLAENTISELANLDLTKSYNQELIVTILK
                     QFLKIEIERTETHEEKFTFGRYKVPDSEKLKQYHRSILKLLANLYNIGSCETRFYIEK
                     MLYDYRRKILTYSESHRN"
BASE COUNT          210 a           61 c           89 g          174 t
ORIGIN      
        1 tatacaaaga ctaattttat taatcagaag gataaaaagg gatctattga ggattcagtt
       61 ttaaatattg tatttgtaac tacacggaca aaatattgta gtcaaattct ccaattattg
      121 ttaaagtact ttgataaaaa tcctaataaa atttctgagg tttatagtat tcttgaagca
      181 aactacgggt tggttacaga aagagaatat attgattata ccttagctga aaataccatt
      241 tctgaacttg ctaatcttga tttgacgaaa agttataacc aagaattaat cgtgacaatt
      301 ttgaagcagt ttcttaaaat tgaaatcgaa agaacagaga ctcatgaaga gaaatttact
      361 tttgggcgct ataaagtccc ggatagtgaa aaattaaaac agtatcatag aagtatacta
      421 aaacttttag cgaatttgta taatataggg agttgtgaaa ctagatttta tattgagaaa
      481 atgctatatg attacagaag aaaaattttg acctattcag aaagtcatcg taat
//
LOCUS       DAVKWZ010000145          511 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00030953
            SAMN33606939-rid17950463.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVKWZ010000145 DAVKWZ010000000
VERSION     DAVKWZ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606939
            Sequence Read Archive: SRR23726237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722114.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722114.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:51:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,161
            CDSs (total)                      :: 2,114
            Genes (coding)                    :: 1,978
            CDSs (with protein)               :: 1,978
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 64 of 136
            Pseudo Genes (internal stop)      :: 46 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606939-rid17950463.denovo.145"
                     /strain="AUSMDU00030953"
                     /serotype="23B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2019"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST439"
     gene            <1..>511
                     /locus_tag="VW854_002161"
     CDS             <1..>511
                     /locus_tag="VW854_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV0263295.1"
                     /translation="DDEIQVAKLAGKYTTEDGYIFDPRDITSDEGDAYVTPHMTHSHW
                     IKKDSLSEAERAAAQAYAKEKGLTPPSTDHQDSGNTEAKGAEAIYNRVKAAKKVPLDR
                     MPYNLQYTVEVKNGSLIIPHYDHYHNIKFEWFDEGLYEAPKGYTLEDLLATVKYYVEH
                     PNERPHSDNG"
BASE COUNT          164 a          104 c          118 g          125 t
ORIGIN      
        1 ctgatgatga gattcaagta gccaagttgg caggcaagta cacaacagaa gacggttata
       61 tctttgatcc tcgtgatata accagtgatg agggggatgc ctatgtaact ccacatatga
      121 cccatagcca ctggattaaa aaagatagtt tgtctgaagc tgagagagcg gcagcccagg
      181 cttatgctaa agagaaaggt ttgacgcctc cttcgacaga ccatcaggat tcaggaaata
      241 ctgaggcaaa aggagcagaa gctatctaca accgcgtgaa agcagctaag aaggtgccac
      301 ttgatcgtat gccttacaat cttcaatata ctgtagaagt caaaaacggt agtttaatca
      361 tacctcatta tgaccattac cataacatca aatttgagtg gtttgacgaa ggcctttatg
      421 aggcacctaa ggggtatact cttgaggatc ttttggcgac tgtcaagtac tatgtcgaac
      481 atccaaacga acgtccgcat tcagataatg g
//
